LOCUS       DAMJZE010000001        67446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJZE010000001 DAMJZE010000000
VERSION     DAMJZE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.001"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(375..1097)
                     /locus_tag="PC541_000001"
     CDS             complement(375..1097)
                     /locus_tag="PC541_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2837492.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1156..1833)
                     /locus_tag="PC541_000002"
     CDS             complement(1156..1833)
                     /locus_tag="PC541_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2837493.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1861..2676)
                     /locus_tag="PC541_000003"
     CDS             complement(1861..2676)
                     /locus_tag="PC541_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2837494.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2902..3112)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3830..5896)
                     /locus_tag="PC541_000004"
     CDS             complement(3830..5896)
                     /locus_tag="PC541_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2837495.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5973..9002)
                     /locus_tag="PC541_000005"
     CDS             complement(5973..9002)
                     /locus_tag="PC541_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2837496.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(9055..10515)
                     /locus_tag="PC541_000006"
     CDS             complement(9055..10515)
                     /locus_tag="PC541_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2837497.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10607..10981)
                     /locus_tag="PC541_000007"
     CDS             complement(10607..10981)
                     /locus_tag="PC541_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837498.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(11157..11897)
                     /locus_tag="PC541_000008"
     CDS             complement(11157..11897)
                     /locus_tag="PC541_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2837499.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11918..12706)
                     /locus_tag="PC541_000009"
     CDS             complement(11918..12706)
                     /locus_tag="PC541_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2837500.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12794..12884)
                     /locus_tag="PC541_000010"
     tRNA            complement(12794..12884)
                     /locus_tag="PC541_000010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12848..12850),aa:Ser,seq:gct)
     gene            13143..13907
                     /locus_tag="PC541_000011"
     CDS             13143..13907
                     /locus_tag="PC541_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2837501.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(14188..15303)
                     /locus_tag="PC541_000012"
     CDS             complement(14188..15303)
                     /locus_tag="PC541_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2837502.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15344..16870)
                     /gene="glpK"
                     /locus_tag="PC541_000013"
     CDS             complement(15344..16870)
                     /gene="glpK"
                     /locus_tag="PC541_000013"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2837503.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            17163..18260
                     /locus_tag="PC541_000014"
     CDS             17163..18260
                     /locus_tag="PC541_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2837504.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(18751..19263)
                     /locus_tag="PC541_000015"
     CDS             complement(18751..19263)
                     /locus_tag="PC541_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837505.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            19816..20697
                     /locus_tag="PC541_000016"
     CDS             19816..20697
                     /locus_tag="PC541_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2837506.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(21010..21789)
                     /gene="hisJ"
                     /locus_tag="PC541_000017"
     CDS             complement(21010..21789)
                     /gene="hisJ"
                     /locus_tag="PC541_000017"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2837507.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(22335..22685)
                     /locus_tag="PC541_000018"
     CDS             complement(22335..22685)
                     /locus_tag="PC541_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2837508.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(22701..23117)
                     /locus_tag="PC541_000019"
     CDS             complement(22701..23117)
                     /locus_tag="PC541_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2837509.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(23136..23327)
                     /locus_tag="PC541_000020"
     CDS             complement(23136..23327)
                     /locus_tag="PC541_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2837510.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(23478..24293)
                     /locus_tag="PC541_000021"
     CDS             complement(23478..24293)
                     /locus_tag="PC541_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2837511.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(24340..25278)
                     /locus_tag="PC541_000022"
     CDS             complement(24340..25278)
                     /locus_tag="PC541_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2837512.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(25275..26147)
                     /locus_tag="PC541_000023"
     CDS             complement(25275..26147)
                     /locus_tag="PC541_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2837513.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(26172..28100)
                     /locus_tag="PC541_000024"
     CDS             complement(26172..28100)
                     /locus_tag="PC541_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2837514.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(28212..28589)
                     /gene="gcvH"
                     /locus_tag="PC541_000025"
     CDS             complement(28212..28589)
                     /gene="gcvH"
                     /locus_tag="PC541_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2837515.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(28676..30802)
                     /gene="cdhC"
                     /locus_tag="PC541_000026"
     CDS             complement(28676..30802)
                     /gene="cdhC"
                     /locus_tag="PC541_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2837516.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(30859..31665)
                     /locus_tag="PC541_000027"
     CDS             complement(30859..31665)
                     /locus_tag="PC541_000027"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2837517.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(31715..33082)
                     /locus_tag="PC541_000028"
     CDS             complement(31715..33082)
                     /locus_tag="PC541_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2837518.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(33113..34057)
                     /locus_tag="PC541_000029"
     CDS             complement(33113..34057)
                     /locus_tag="PC541_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2837519.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(34077..34847)
                     /locus_tag="PC541_000030"
     CDS             complement(34077..34847)
                     /locus_tag="PC541_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2837520.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(34903..36288)
                     /gene="lpdA"
                     /locus_tag="PC541_000031"
     CDS             complement(34903..36288)
                     /gene="lpdA"
                     /locus_tag="PC541_000031"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2837521.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(36332..37213)
                     /locus_tag="PC541_000032"
     CDS             complement(36332..37213)
                     /locus_tag="PC541_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2837522.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(37248..37922)
                     /locus_tag="PC541_000033"
     CDS             complement(37248..37922)
                     /locus_tag="PC541_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2837523.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(37959..38831)
                     /locus_tag="PC541_000034"
     CDS             complement(37959..38831)
                     /locus_tag="PC541_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2837524.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(38923..39555)
                     /locus_tag="PC541_000035"
     CDS             complement(38923..39555)
                     /locus_tag="PC541_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2837525.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(39660..41336)
                     /locus_tag="PC541_000036"
     CDS             complement(39660..41336)
                     /locus_tag="PC541_000036"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2837526.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(41585..42364)
                     /locus_tag="PC541_000037"
     CDS             complement(41585..42364)
                     /locus_tag="PC541_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2837527.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(42674..44593)
                     /gene="cooS"
                     /locus_tag="PC541_000038"
     CDS             complement(42674..44593)
                     /gene="cooS"
                     /locus_tag="PC541_000038"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2837528.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(45200..46111)
                     /locus_tag="PC541_000039"
     CDS             complement(45200..46111)
                     /locus_tag="PC541_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2837529.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(46227..46805)
                     /locus_tag="PC541_000040"
     CDS             complement(46227..46805)
                     /locus_tag="PC541_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2837530.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            47031..47954
                     /locus_tag="PC541_000041"
     CDS             47031..47954
                     /locus_tag="PC541_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2837531.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(48126..49268)
                     /locus_tag="PC541_000042"
     CDS             complement(48126..49268)
                     /locus_tag="PC541_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2837532.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(49286..51040)
                     /locus_tag="PC541_000043"
     CDS             complement(49286..51040)
                     /locus_tag="PC541_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2837533.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            51176..51382
                     /locus_tag="PC541_000044"
     CDS             51176..51382
                     /locus_tag="PC541_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837534.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(51387..52310)
                     /locus_tag="PC541_000045"
     CDS             complement(51387..52310)
                     /locus_tag="PC541_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837535.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(52564..54264)
                     /locus_tag="PC541_000046"
     CDS             complement(52564..54264)
                     /locus_tag="PC541_000046"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2837536.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(54726..55139)
                     /locus_tag="PC541_000047"
     CDS             complement(54726..55139)
                     /locus_tag="PC541_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2837537.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(55207..57585)
                     /locus_tag="PC541_000048"
     CDS             complement(55207..57585)
                     /locus_tag="PC541_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2837538.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            57904..59355
                     /locus_tag="PC541_000049"
     CDS             57904..59355
                     /locus_tag="PC541_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2837539.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(59559..62072)
                     /locus_tag="PC541_000050"
     CDS             complement(59559..62072)
                     /locus_tag="PC541_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2837540.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(62295..63116)
                     /locus_tag="PC541_000051"
     CDS             complement(62295..63116)
                     /locus_tag="PC541_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2837541.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(63634..64110)
                     /locus_tag="PC541_000052"
     CDS             complement(63634..64110)
                     /locus_tag="PC541_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2837542.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(64144..64722)
                     /locus_tag="PC541_000053"
     CDS             complement(64144..64722)
                     /locus_tag="PC541_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2837543.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(64826..67408)
                     /locus_tag="PC541_000054"
     CDS             complement(64826..67408)
                     /locus_tag="PC541_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2837544.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
BASE COUNT        20970 a        12631 c         7418 g        26427 t
ORIGIN      
        1 ttactataaa agttaaattt ataagcttac ttgttataaa aataactttt ataatttatt
       61 tcaaaaaaaa tattgatttt tgtgtcgcat tttgttataa taaattaagg ttatccccct
      121 ggtaacccta gttagttaat atatctattc ccaataccct ttcataagga tatattttat
      181 atttattaaa aatcggatta gtaacggaaa ttaattcggt ggaaaaaaag agcttacccc
      241 gagctctttt ttttctgcaa aaaatcgtaa aaataaagtt aatattaata taactttaat
      301 tttaaaatta atattaacca tcactaaaaa aggatgtatt gaatttctaa tatcaataac
      361 atccttttta tatattatag cacttttgct aaaaatgctt ttgttctttc atgttttgga
      421 tttccgaaaa cttcttctgg agtattatct tcaagtatac ttcctccatc tataaaaata
      481 actctatctg caacttcctt agcaaatccc atctcatgag ttacaattgc cattgtcata
      541 ccttctttag caagttcttt tataacatcc aaaacttcgt ttaccatttc tgggtcaaga
      601 gcagatgttg gctcatcaaa caacatcata tctggttcca tagcaagtgc tctagctatt
      661 gctattcttt gcttttgtcc tccagataat tgtgatggat atgaatcttt tttatctaaa
      721 agaccaactc tactcaacaa aatttctgct tttttttctg cttcttcctt agtcatcttt
      781 tttaatttaa ttggtgctaa tgttatatta tctaaaatag tcttatgtgg gaataaatta
      841 aagttctgaa acaccatacc tatattttgt ctaacctcat caatattagt ttttttatcg
      901 accaaatttt ttccttcaaa tattatttct ccacctgtag gtatttctag taaattcata
      961 catctaagaa gtgtactttt accagaacca cttggaccta ctataaccat tatttcgcct
     1021 ttagcgattt ctaagtctat attttttaat acatttaaat ctccaaaaga tttacttaaa
     1081 ttttttattg taatcatatt atcactttcc tttttactat tctatgtttt ccctaaatgc
     1141 taaacatgct ctcatctagt tagacacaga tagtttcttt tctaataatc caactaatct
     1201 tgatagagta aatgttataa caaagtaaat tgctgctgct actattaatg gctctaatgc
     1261 tctaaatgct gcatttttta ctatatcagc agtacgcatt atctcaacaa ctcctattac
     1321 agatattatt gaagattctt ttacaagtgt aataaactca tttgctaatg aaggtaatat
     1381 attttttata gcttgaggta ttattatata tctcatagtt gaccaataat ttaatcctaa
     1441 tgatctactt gcttccattt gtccattatc aactgattgt atacctgatc taagtatctc
     1501 tgccacgtat gcaccagagt ttactgataa tgcaaatgca ctagctataa attcacttgg
     1561 tataccaaac aacataggga atcttattcc taattgaggt aaaccaaacc atactatata
     1621 tatctgtact aataatggtg tccctcttaa aacctcaata tagattgatg atattgctct
     1681 taataccttg ctctttgata tttttgccat acagataatt atacctatta taaatccaaa
     1741 gcataatgcc acaagagaaa ttgatactgt cacacctgtt ccttccaaaa aagaagtccc
     1801 aaatctactt aaaaaactaa aatctaaact cataatttca tctcccacat aactttctta
     1861 ctaatttgaa gcagcttcag tagatgcttc atttaaaaat tcttctatct tcttgccatc
     1921 ttgtaattct tttatctttt tgtttaataa ttctataaaa tctttgttat tgtcagattt
     1981 tttgattgct gctgccatac cttctgttac atctttccca acttcagtat ttgccatttt
     2041 tattccatca tattttttaa catttattaa agaaactgct tcattagtaa caactgcatc
     2101 tatgtttcca ttttttaatt ctgtaattaa atcaggtatt gctttcaaag attttgtagt
     2161 tgtcattttt aaagtgttaa ctacatattc ttcttggata cttcctgctt gaactccaac
     2221 tctaagtttc tttaaatcat cttcagtttt tactttgtct atatctgcat ctttaactat
     2281 aactgcattt ctacttaaat aatataattc tgaaaaatca acactctttt ctctctctgg
     2341 tgttggagac atacctgcaa gaacaatatc aacttggtct gcatttaaag cacctattaa
     2401 tccatcaaat gacatatcct taatttcaac ctttacacca agctcttttg caaattcttc
     2461 agccaacatt atatcgaatc cttttattga atcttttcca tctactacct tatgaaattc
     2521 aaatggagga aattctgcac tagtaccaac aactaatttt ccattgtctt ttatttgttc
     2581 taactttgtt ttttctcctc caccactaca tcctactagc gataatgtca atcctaatac
     2641 taatcctaaa cttaataatt ttttaaatac tttcatttta actacccctc tcttttaatt
     2701 ataatttccc tcagatttta atacttaaat gtttcgagtt acaaaaaaag tctctcagtc
     2761 tttaatgact aagagacgac tttaccgcgt taccactctt attgataaat acttatatta
     2821 ctttcaaaat ttgtgactgt tagcatttaa gctgtatgga tacttttatt tttaaatcaa
     2881 aaaagtccct tagctttaca gctaagagac gaattatatc cgcgttacca ctctttttga
     2941 taagtaagta cttatcctct caatccctta aggcgggtaa accaattatc atactaaagt
     3001 ttctgataat ctcctccaga gctctcttca catagacttc attgctaggc ttacaccaac
     3061 ccctaactcg ctgtaaatcc atatatgcta ctttctctat cattggattt gtttccttaa
     3121 attgttaata caattgtact tccttataat atttttgtca ataagttttt tattttttta
     3181 ataattttta cttgtaaatt atctaaatac tactgtttat atttattgat attagttttg
     3241 taattataaa tagtatattt catattttta gatatttctt aaataatact aaataatatc
     3301 tttcacattg ttgctttttc ttaaacaata ctaaataata tctttcacat tgttgctttt
     3361 cttaaattac actaaataat atctttcaca ctgttgcttt ttctttaata atactaaata
     3421 gtatctttca cactgttgcc ctttctttaa taatactaaa taatatcttt cacattgttg
     3481 ctttttctta aattacacta aataatatct ttcacactgt tgctttttct ttaataatac
     3541 taaatagtat ctttcacact gtttctcttt ctttaataat actaaataat atctttcaca
     3601 ttgttgcttt ttcttaaatt acactaaata atatctttca cattgttgct tttctttaat
     3661 aatactaaat aatattttcc acattgttgc tttttctaaa caactataaa taatatatta
     3721 aacccttatt tctttttcta ataagttaaa taatacattt tacacatatc tatttgtctt
     3781 gcacataata aataatacat tatatttcta tctaaattta gattctaaat taaatagtat
     3841 aaattatatt cttcaataaa caagttttca aatccaagat tcgctctcta tctttaaact
     3901 ttattgtaaa cataattcca tcttttttaa ttatttctcc ttcatgaaat tttttatggt
     3961 ataccttatc ccctacacca atactgtcaa tttctttttt cgttggagat tttatatcat
     4021 taataaataa tgatttatca actttcttac catacttatt taaagtagag cttatacata
     4081 acttttcttc agctcttgta atagcaacat acataagtct tctttcttct tctaattgtt
     4141 cttctttttt ctcttcctta caaatatcat atgatttttc atgaggtatt gttccttcat
     4201 ttacacctat aatataaaca tttctaaatt ctaatccttt tgcactatgc atagttgtaa
     4261 atatgacacc ctcagtctgc ttattcttgt tattttccgt taactcagtc tttactctat
     4321 caatatgctc taaaaactct gtaacagtat taaaattagt tgctgaactc tcaagttcat
     4381 ttaatatctc tacaagtcca ttagtcttta tctttctgtt gctacaataa tctaaaacat
     4441 atctatcata atcaagacta gttctaatat aagatattgc atttttagga tttaaagtat
     4501 ttaaataact taaatctatt tccaaatcat ttattgtttt aacttgttta ggatgaagat
     4561 tacacttatt tattaatgct gttataaagt ctttttcatc cttaaccata tttatactgt
     4621 ctttggatat atatctaaaa ggcttgttta taattctcaa ccaatcttta tttgattttg
     4681 gattaatacc tattcttaaa taggctagta tgtcttgact tgcccaatgg tcatatattg
     4741 taattactga gtcttttact acaaatggaa tcctcatatc cataaataca tctactaatg
     4801 ctcttgactg tatattagtt ctataaatta ctgcaaaatc tgaatactct acataatctt
     4861 tttgtatttc gtcaattatt tccctggcaa tatataaggc ttcttcctct gaatcatgcg
     4921 gagaaatata ggtaacactt cctccatccc cctgactaca cttaatgatt ttttcatatc
     4981 tatttttatt tttgtcgata agtctatttg cagtatgaac tatctcactt ttagacctgt
     5041 aatttatatc taatattatt tttttagtat tattaaagta ttgttcaaat tcaagcaaaa
     5101 aatctggtct agcacctcta aatccatata tactttggtc ttcatctcca actgcaaata
     5161 tattgtttaa tgggctacaa attaacttta aaacttcaaa ctgtacttta tttatatctt
     5221 gaaattcatc tatcaatata tatttataca catttctaac catttctagt gcagacttat
     5281 tgttcaaaag taaataataa gttcttataa gcatatcatc aaaatctatt ttattaactt
     5341 tagatttttg ttcttcgtat aagttgtata cttttaaaaa ctcatctttt gttaatactt
     5401 ctgaattaaa ttcattttta tccataagtt cattttttac ataggaaatt tcatttataa
     5461 cttgtcctat attctcatca tcatcagcat tctctatatt taaacttttt aggattgcct
     5521 tgattgtcat tcgctttgac ttttcatcaa atatactgtc taagttgtat ctttcaaagt
     5581 atcttagaat tttaaaaaat acagagtgaa aagtaccata ggttacctta ttaagtctta
     5641 tgtcatcact taaattcaaa gctctatttt tcatttctat tgaagatgcc ttggtaaaac
     5701 ttattgccaa gattcttgtt ggtgctatgt ttttgtttac aaccatattg gctattctat
     5761 atgtaattac tctggtctta cctgaaccag gccctgcaag tatcatgcaa ggcccatcta
     5821 tatgctcaac tgcttctaat tgattttcat ttaactttct tatatctata tttgttgtga
     5881 ttattagctc caccaccttg atttataatt atattatttt ctttttaatt ataaactaaa
     5941 agttcataaa aataaattta atttttcctc tattaaacca catcattcct attttcatga
     6001 catatcttga gttctttttt aattttgtcg cttatatctt caggtataaa tgatggttta
     6061 ctaatatagt agccttgcat ataatctact ccaagattaa ttagtgtttc catttcacct
     6121 tttgtttcta caccttctgc tatcacttta atatttcttt cgttagaata tgagataaga
     6181 ttttttaata ttttctgtct gttttcatct ttatcaatac ctcttacaat agacatatca
     6241 atctttacat agtctggcat tatatataat agtacagcat ctccgttgta acctgtccca
     6301 aagtcatcta aagcaatttc tatattccaa tggtctatat atgctttctt tttctcaata
     6361 aaatcttcat taatcttatc attctctgtt atttctagta ctactctatt taaatattca
     6421 ccatataaat cttcaatttc ctcaaaatct acttttgaca aattattatt aggaatagaa
     6481 tttaagaaca gtttgcaatc gccaaatttt tctttgtatt ttttaaatga tttaagtgct
     6541 ttaaaccatg taatacgctc aatttgatat aatttagact gtgaacgagc taatctaatg
     6601 acatctattg gcgaactaaa agtttttatt ttaggtctca taagtgcttc atatgcaaaa
     6661 actttacctt tacaatctac aataggctga aatgcatact caactaattg accatcaatt
     6721 aacttgttta attcttctct attactcaat aagaatgaat ccttattata tatattaata
     6781 tcaaattcac taacttcacc cttgattgta tttttaattt gatacatagc aaaatctgcg
     6841 tatctcatta attcatcata gttggttgag tcatcagggt accaggaaat tcctgctgat
     6901 gcccttattt taaattctgt tccatcatga agttttaata aggtattatt catctgtttc
     6961 ttaacagaat taataatatc tcttatacta tctttacttt catatccata tataaataca
     7021 taaaactcat cacctgaaag tctagatact ataccattcc atgatataaa cttcttaagt
     7081 atgtctgcag cacacctaat atattcatca ccagcatcat gtccatatgt atcattaata
     7141 tattttagat tatctaaatc ccacataata aatgctgcta tcttaagttc atctttattc
     7201 ttaaattttt cttgtatctg tgaatgaaaa gctctcctat ttaaaagatt cgttaaaata
     7261 tcaaaatctc tttcatattc tatcttcctt ttctcaataa attcttttgt tacatcaaca
     7321 acaacaccaa gaacctttag ctcatcttca aagatattca ttcttatcca cttaagtttt
     7381 ttatctttag attcaaactt atatatatat gtattttctg tttcataact ttcttgcaaa
     7441 tatttatcta gtccccttaa ttttttcagg aacttatttt tttccatata tccatattct
     7501 tcaaatttga tttcagtagt aaacacgcta aaaaattctt ttgtacaaaa aacattctct
     7561 gcgtttttgt ataactcaaa agctccaatt ggcatatcta ccatttcaat aatctgtgat
     7621 aattttgacg aagaatctgc aacatttgca ctgagcaact ctatagctga agataattca
     7681 tctatttcag atatatttat cttatcaaga tttactggtt ttgatggatt actgtttcta
     7741 actttgttgg ctaaaagaac aattggtttt gtaaataatc ttgatgcaat ataaatacct
     7801 attagaccta ttaataaaga aattgctata gatattataa ctaatttctc tacccttttt
     7861 gaaaatgaca aaagactttt ttcttcaata atacccaaaa gagcccaacg ttcattttca
     7921 aatggagtat tggagttata taaatctaag tattgtacac tagcataaat gttgccatta
     7981 ccacattcat tatctttatt tttaatctta tatatatttt tatacgattc cttctgctct
     8041 aaattagaat gtaaaccatc ccaaaatttt tcttttaatg agtatccatt tgaaattaca
     8101 ttttcaaagg ccatatcatt atttctatct acacctaaaa tataattacc ctttttattc
     8161 ccatttatct ctgagtatgg aagtaattct cttaaataat ttacacttaa atctattcca
     8221 agtactccat atacagttcc atcttcataa ataaggggaa ctgtatatgt cataagccta
     8281 tcacctgcat ttgtcaataa aaacttactt ccccagtatc ctaaattaga acttgataca
     8341 tttggattat caattgcagc tctaaatggt ttataaaaaa aatcatctcc atcttgctca
     8401 ttttcacttt taaatgaaaa cttaggttcc caataactgt ccatagatat tccaaaactt
     8461 cttgctatat ctgatgaacc tctctcaatt aacaaatcag aattatcatt tggattaaac
     8521 ttagggtcta aatcccttat atacaatcct gtcctactta tttcaccttt actatctatt
     8581 gacttatcgt taatattttt gtcacttagg attataaaag ctcctgtaac agaatttttt
     8641 ctcaaaagat atattaaatc atttgatatg ctttttataa tatctttgtt aatttcttca
     8701 ttgttgccta catcctcaat taaggcattt ttttcattta atactttttt tacagaggaa
     8761 ttaattgtag cttccgtttc acttgtattt gaccatcttt gtaacatttc attttgtata
     8821 taatttttac gattaattac tctttcattt aagatgtcaa acgaattatc atttaatttc
     8881 ttaatagttc ctccccataa aattgtggca caaaataaac ttgtttgaat tagcataaca
     8941 ataattaggg gaaatatcat ctttttcatt attgaattgt tttttcttag attgttttcc
     9001 atacccattc cacctatacc tttgtgctcg tcaacacact aactgccaat tcatctattt
     9061 ttttatagca ttttcaagct cagccttaaa actttcaaac caagttttaa aatttttatc
     9121 attattaaat tgtgctactg catccttctt agaagttcca ctttttacta attcatttat
     9181 tttatttgaa tcttctttag ctttatcaag cattgaatat tcaagtattt gtctagcttt
     9241 agttccacca tcaaatgctt tattagtata taaattacaa ctgttaatct gttcaataga
     9301 tgaggctatt gaaccttcaa ctttatcaga caaactcaaa ttatctttct tcataacttc
     9361 ttttataaca tccaaatcat tcgttttttt ctttactggt aagtatccag aagcaataga
     9421 gaattttata ttaggttctt tatctgtaaa ccattttaaa aattctactg atgcatattc
     9481 ttcttcttta gtagattttg ttacagccat tcctgcacct tgttgtacag catattttcc
     9541 ttctccttta aaacgtggtg cttgaaaaac agatgtttca atattataac tctcattatc
     9601 atttagcatt actctatctg gaaagtatgc tgctcctgaa ctagaaccta caagagctaa
     9661 tatatcacct gtttttgcat catcagaacg aaacttacca taagaagcaa aatatccatt
     9721 tatatatggt acatagaaat tatcccatag ttttttcata actttttcat caatatttag
     9781 cgtaacttta ccatctttaa cagaaaaaat ttctactcct agttgtttac ttcctataat
     9841 aatataattt gctaaagcat ctcttccaaa aaatgcttta ccatcattag gctctttcgt
     9901 taaactatct gtccattcat aatatttttt tgatgtctct actaatcctt caattgtttt
     9961 tatatcattt atatttgaat ttgtagcttt tgcaaattta tcccaatcag ttttatttaa
    10021 catcaaaatt tcagttgatt ttgcaattgg gaaaagttta aactcatcct tagaatttat
    10081 tctgccttct tctacatatt cgtctatgta ttcatttaat tcttctttac ttatgtactt
    10141 atccatatct actagtaacc ctaatttatc catttcatat gcagtatctg agtatgctgc
    10201 aaatatattt ggtacatctt cagaaccaac ttttttattt gcagaatcta aaactttctc
    10261 tgataaatca actacattac cttgactgaa tgcttcaaca ataattcctt tttcagcacc
    10321 tacagtgtca ttaaattcct taacaagctt atcaaaagcc attttctgct gtccattata
    10381 ataatgccaa atttgtattg atgtcggatt ttctgggtct aacttataat tatcttcctt
    10441 ctttaaacta catcctgtaa gtaataaaac aatcattgat gtgataatta attttgcaaa
    10501 cttacaccta ttcataaaat ctactccttc aaattataaa atttataata gcattaaaac
    10561 tgtaacttca ctatttatat taattataga acgtttattg atatatttaa acaggattta
    10621 caacatattt aaaagcaatt aagaaacata ctattacaaa tatagaaaat aaagctttta
    10681 cataattttt atttctaaat gcaccaaatg acaccaatcc ttgttgaata gctactgtaa
    10741 ttactaaaag tatcattatt atattaagtt gaaccagttt aaataagtaa agcaatgaaa
    10801 acactactag aagtacacat aatacaatac tagatttatt aataatatca tcaatcttat
    10861 ctactttctt agattgctta ttaattttat ttaatttttc tttatttaaa ttctcaactt
    10921 ttttattata cctttctctt gttttatctt tatatttagt atttttattt ttatttccca
    10981 tttattttcc atcctctata aattttttaa ttgagcttat acattattta aatattatat
    11041 atttaagata ccatatattc ctacaaaaag gtaacttact atgtatctat ttttgatttt
    11101 cgcataaaaa agccatctcc tttagaatta attctgtatt gagacagcta atttaactat
    11161 ttatcctttg aaacattttc tacattatca tatttactat caactaaaac tatatcaact
    11221 cttctatttt ttgacctacc ttcttcagat gaattttctg ctattggtct aaactcacca
    11281 tatcctacag cagataattt atctggtgct atacctgcat tgtcaataag aagttgaaca
    11341 acattggttg ctctcataac tgataaatcc caatttgact taaactctga attttttata
    11401 gacatattgt ctgtatggcc ttctactctc atgtaactat tcatttcatt taacatctta
    11461 cctatttgaa taatcttatc tctagaatta tccttcacaa tagctttccc tgtatcaaat
    11521 agtattgtat ctttaaggct tattactaat cctctatctt gaactcttaa tgatactgaa
    11581 ttagccatac cattttcgtt taaatacttc tgaatttctt caccaatttt tttaaactta
    11641 gcattttgta aagtatctaa atcattactt cctggttcaa ctactggttc tatcttgcta
    11701 ttaccacctt caataactcc agaagaacca ccaaaagcag aatttagaga ttgagataat
    11761 tgtttatatt tctccgcatc aacattactc atagaataca ttattacaaa gaaaatcata
    11821 agtaatgtta tgaggtctga ataagttaaa agccatcgct cattgttttc ttctttttca
    11881 tcttcatcat acaacataaa cattccatcc ttttatatta ttcagctcta ccaccaattt
    11941 cttcaagttc aattagttct tttgaattta aaaatatttt taatttttct gtcattaaat
    12001 tagaattatc accctcaagt atggataata ttgcttctat caccaaaagt ctttcactta
    12061 cttcttgttg atttatagcc ttaagttttg atgctattgg taaccatatt aaattggcag
    12121 aaccaacacc atacaaagta gccaagaatg caacagaaat ttgaggtcct aatgcatctg
    12181 gattacttga aagattacct aaaatatgta ctaatcccat aactgtacct attatcccca
    12241 gtgtaggtgc atatccacct gctgaatcaa aaattgctat tccttcttta tgtcgttttg
    12301 atgttatctg tatctgtgat tccaatatat ttcttatagt aactgcatcc actccatcta
    12361 ccataagttc aagccctttt ctaataaaag ggtctaattc atcattctca cttatttctt
    12421 tgtcaatact aagaagcccc tctttcctag cctttgttgc aatttctttg aaatagatta
    12481 tattttctat gttattaggc tttttatttt taaaagctaa taacattatc tttatagact
    12541 ttttaaacac agggaatgga gtcgaagctc caacagctcc tatagttcca ccaaacacaa
    12601 caagtgctga tgaaaataca aataatgctc caacatgtcc cccatcaata acaaaagcaa
    12661 ctaccaaaga tgctatcccc actatgaaaa aaattaaagt tgtcatctat tccctccttt
    12721 ctttagttat acatatctaa tcatttatga cattataaaa agactatctt taataaaaat
    12781 agcctttaat aattggcgga gaaggaggga ttcgaaccct cgcaccggtt acccagccta
    12841 atcccttagc aggggatcct cttgagccac ttgagtactt ctccatatct ctatactaat
    12901 aaattatata ataaaatcat attttagtca attgttttat aaaatttgag ttattttgaa
    12961 ttattaaaca tctattttta cttttttgct taaatgtaag ttttaaatat ggtaaaatta
    13021 tcttgcataa cacttataat attatttgat aaatatacat acattaacaa atatacctaa
    13081 actctgactt aattgtaagt tatgccaaaa ctaaaataat actagtccaa ggaggatatt
    13141 ttatgttacc acaggaaaga tatgagaaga ttttaagtat tctcaaatca gataatataa
    13201 ttaaaataaa tgacatagtt caaatgtttg atatatcaat agaaactgca cgaagagatt
    13261 tagcccatat ggagagtatc ggtcttttaa aaagagttta tggtggtgct ataccagtta
    13321 tagcaagtag cgtagaactt aactatgtta aaagaaaaaa aataaactca aacgataaaa
    13381 aacagattgc tattaaatgt agtgagttta taaatacaaa tgatactatt tttatggata
    13441 ctggaactac tgtacttgaa gtttcaaaaa atttgaaaga taaaaagaat ctaactgtaa
    13501 taaccaactc aattttagtt gcaaacgaac ttatgaatac taatataaaa gtatatgtgc
    13561 ttgggggcta tcttagagaa actgaaggtt ctttttctgg tccgcttaca ctgtcagcta
    13621 ttgaacaatt taatgttgat aaagctattt taggcgctgg aggtatcact cttgaaaatg
    13681 gaatctctga ctatcactta gaagaagcac ttgttagaaa aaaaatgatt gaaaggtcta
    13741 gaaaatctat agtagttgtc gaacctccta agtttggtat aaattcattt gcatcagtag
    13801 ctcctataga acatatagat actattatta cctctgctag tatagaccat aatataataa
    13861 tagagttttc tcaaaaagga atacctttag tcatagctga taattagctt atatgattga
    13921 tttttagttc cttgatgtaa taaattaaaa ttaaggagcc aaataattat ttatttagct
    13981 ccatttatgt ctaaactata ctttatattt taattatgta actactttca gttagcttat
    14041 catttttagt tatttgattg ccattttatt taatttataa gttttagtta ttcgattatt
    14101 ttttaattgt ctgtcttttt aatttatttt tagttattta aactcctact ttaaaataat
    14161 ttaaatttga cttaagtatt gtttattcta cactgcatct ttcttcattt taatactttg
    14221 ttcttcttcc aaggttaaat cttttgtcat aagtatagca agtgcaattg ccaatactcc
    14281 tccaaccaat tttccaatta caacaggtgt aatcatctct ggaactactc ctgctgtaaa
    14341 tcctaaatgg tcccccaaag ctgctgtagc acaaaccagc caagcagtat ttactacttt
    14401 cccctttgga ctcatatctt tcatcatttt atatactgga atactattgg ctaaagtaaa
    14461 aactaatcct gctgcactag tggcatccat acctaatttt ccacccacta ttgtaagtgg
    14521 tttattaaga actttaacta ataaatgtaa tattggaaaa gtacctaata acacgattgc
    14581 aatacttcca attacttgaa taccttctct tataggtgtc atgcctttta taagtactat
    14641 acctgttatt tcttgaaatg ctgctgctcc caaacctatc gttatcaata tagatataaa
    14701 ttttccaaaa attaatgacc catttatcat aagagtagaa ttaaatttta aacctactgc
    14761 caatattact gccaatatta tcactggtat catatttata attaccattg ataagttaaa
    14821 tcctgctatt aatcctccta taatacctcc aaagggtatt gttatcaagc ctatcagtaa
    14881 cccttttgca aagaatggtc tgtcttcata ttctataaga cctagtccta ctggaattga
    14941 aaatactaat gtacatccaa gcatggatga cacaaccaat cctgcaagca ttcctgcctc
    15001 tttattgtgt gcaagttcca tagccaatgg ataccctccc atatcattcg ctaaaatagt
    15061 tgcaaacata gatgggtcag ctccacagag attgaagatt ggtattatta taggacctag
    15121 tacatctgaa attactggag ctaaacaaac tataccaacc attcctaaag ccaatggacc
    15181 cattgaatta aacccttctt caaactgctc gccaagtcca aatttatttc caattattct
    15241 atctaacgct cctaaaagca ctcccacagc cattatgtat aatataattt tatcaaaggg
    15301 catttttaat tcctcctata catgtaaaat actctattgt aacttaatta gcacacacct
    15361 ttttggtatc gttttcccaa ttcatagctc tttttactgc ttggttccat ccaagtaata
    15421 tttcttctct tttttgagta gtcatatttg gaatatattt tttgcttatt gtaaattcac
    15481 ttctaatttc atcaatactg ttccagacac cacaagaaag acctgccata tatcctactc
    15541 caagtactgt cgtctcagga ttttctgcaa tttcaattgg tatacctaat atgtcggatt
    15601 gaaattgcat tgtaaagtta tttttagtaa ttccaccatc tactctcaac atttttattt
    15661 ttttccctga ggctaagttc atagaatcaa caacatcttt gacttgaagt gcaacagact
    15721 caagagtcgc tcttactata tgctcttttt tagtacttcc tgttatccca accattgtac
    15781 ctctagcata ttggtcccaa taaggtgctg ctatacctgc aaatgctggt acaaaatata
    15841 cacctccagt acttgatatg gaatttgcca tgtcatcagt ttctgaataa ttttttataa
    15901 ctccaatgcc atctctaagc cattgtattg cagcacctgc tatatacact ccaccatcat
    15961 aagcatatgt aactttatct cctattttcc aagcaacagt tgtcaaaata ccattacctg
    16021 ctatatttag ttcctcacct atattcatat ataggaaaca tcctgtacca taagtatttt
    16081 taaccattcc tggttcaaaa catccattgc caaataaagc tgctggttgg tctaccattg
    16141 aagcagtaat ttttatagaa accccacaaa atgcctcctt atctgtagtt ccaaaatcac
    16201 cactcgtagg cattatatct gctaaaatac ttctaggtac tcctataatg tcaagcaact
    16261 ctctatccca atccatatct cttatattaa aaagcattgt tcttgctgct gtagatacat
    16321 cagttaaaaa agattttcct cctgtcattt tccatattaa ccaactatct aatgttcctg
    16381 caagtacttc tccattttta attttttctt ttacaccttt tacattatct attacccatt
    16441 taatttttgt acctgagaaa tatgggtcta taattaaccc agtcttatct attatcttat
    16501 tttctaaacc tggtatattc tttaaatttt ccacataatc ataagttctc ctgcactgcc
    16561 acacaatagc attgtaaagt ggtttacctg tatttttatc ccataacatt actgtttcac
    16621 cttgattatc tataccaatt ccttttatat cactttcatt gattccattt tcaaaagcct
    16681 gttgtaatac attattactc acagtttttg tattattcca aatttctata gggtcatgtt
    16741 caacatatcc agattgtgga tatatttgag tatgttccat ataatcgcta aaaacaaact
    16801 ctgatttttc gtttatcaat actgctcttg ttcctgttgt tccttggtca attgctaata
    16861 catatttcac tttaaatgtc ctcctttatt ttcagaatta ataaattctt ttttattttt
    16921 tgaatatata atctattcaa gttatttata ttttatatca tcatgtggat ttatgcaata
    16981 ttcattacaa tattttttgt tatttttaat tatagtgatt gaaaaagtat ttttattgaa
    17041 atattgttgc aatatttatt attttattct ataatctagt taaaataaca cataatattt
    17101 taatatttac acataatcag tcattcaaaa atttaaattt aactttttaa ggaggcttta
    17161 caatgaaaaa aacatatcta ttcagtcctg gaccagtaat ggttacagat aaagtcagaa
    17221 attcacttct tcactatgat atatgccata gaggaaatga gtttatggat ttattcaaag
    17281 atacacaaca aaaaatcaat aaactatata atgcaacttc taattactac tcattaattg
    17341 tatctggctc tggaacatct gtaaatgaat gtgtactatc ttctatcttt gacaaagaag
    17401 atgcagttct gctagttagt aatggtgttt ttggagaaag gctagaagaa atattatcta
    17461 cttataatgt aaaaacatat aaacctaatt ttgaatgggc agaatacccg gacctagaca
    17521 ttttagaaaa atacataatt gaaaactctg atataaaagt aattgctatg gtgttccatg
    17581 aaactagttc aggaatgata aatccagtcc ctgaactagg aaaacttgct aagaagtata
    17641 acaaaatatt ttttgtagat gcaataagtg cctcagcagg tgaatatatt gatgtggaag
    17701 agtttaatat agatataata acaggtgttg gtggcaaagc tcttggagca tttccaggtt
    17761 ctgcatacct ttgtgctaaa gagagtattt tacaaaatat taaagaaaat caatgtaaga
    17821 atgtgtatct taatctttat aaacactata aagttgctaa atcatcttct caaacaccaa
    17881 atacacctaa tgttacttta atatttgccc ttaatgaagc tttaactgag tttttggaag
    17941 atgacagtaa aatagaaaga tacaaagaat gttctgctat tttaaggtat ggtatggaag
    18001 aattgggtct aacattttta cttccagata agtatatgtc taatacagta acatctgtat
    18061 ttctacctaa agaaatcaat gtaaatgagt ttatattagg gctagaaagg gaaagtggct
    18121 atgttgtcta tccaggaaaa ggcaaattcc tagatgctaa catattccaa gtagcaaata
    18181 tgggagaaat atatccagaa gattgttata aattcttaga tatcttaaaa tcaaaattgg
    18241 agataaaaaa agttatataa cagtttgttt gtaaaatcct acaatatatc agtataaatt
    18301 aaatagactg atttttttaa attttcctat aataaattat attttatatt tttgtataag
    18361 ggtgaactat aatatgtaaa gttcacccct atttcaaatc tttttccttc aatataatct
    18421 ttttctttta atttatttta tatttttgac gcaatcgttt tttattcaat cattttatat
    18481 tcttgataca atcattctta cacttaatta ttttttgttt ttgacacaat cgttttttgt
    18541 tgaatcattt tatattcttg atacaatcat tcttacactt aattattttt tgtttttgac
    18601 acaatcgttt tttattcagt catttaatat tttcaacaca atcattctta cattctatta
    18661 ttgtttgttt ttgatacaac tgtttttatt cagtcattta atattctcaa cacaattacc
    18721 ttttcattca atcaaagtaa tctttaagca ttatttttca agctcatcaa agtaattata
    18781 tacgataacc tcacctgtct ctttatctgt agtctttata atgcccttag ctccatttac
    18841 ctcttctttt gtaaattcat catctaacac aactttgaat ttatcttcct ttccataacc
    18901 ttgttgaaat tcatctctca aaattgcatt tttttctgct tttggcttca actcaaattc
    18961 aattccagat ttcatttcat caaaataatt taatactaca acctcattat tcattttatt
    19021 agttgtcttt ataatccctt tagctccatt tacttcttct tttgtaaatt catcatccaa
    19081 tgttatatta aatttgtctt cttttccata ttcctcttga aaatccctct tcatttcatt
    19141 gtatttagtt tcaaactctt tactcttgtt taattccata gctgatacat ttgtcaatga
    19201 tgctccaatt gccattactg ttaatgccat tactatacca cttttaaatt tttttacttt
    19261 catatcttac ctcctaaatt aaatgttctc aatgaaatta tataaagaaa atcctaagtt
    19321 ttacagtttc attcttgtaa ctaaaattac tattatgtaa ccaaactttt tgcttcaact
    19381 attccttaaa agttgctttt ttgaggaata gttgaagtta aataattaga ttttatctat
    19441 atttatagat ttaaaatcaa atttatccat ataaataaat acttattttt gcaaaataaa
    19501 aatttttaat tactccaatc cttcttattt acttaaaatc ctatgataag aaatttaaaa
    19561 gtctttacct tctttaacaa taatttattt acttttaaca tacaaaatat agagagtata
    19621 tcaaaactat tttactgaat attctaaaaa taagtacccc ctattagtat atatatttac
    19681 atattaaaaa aaagttttaa ataaagttat attttttaca acagttttgt aattaaaaac
    19741 atattgaagg taaatttaaa ggtactatat actataaata tgtcgacaca ataaatttaa
    19801 aaaggagaac taaatatgaa aaaaagatta ataataatga tattgtctgt tgtcttagta
    19861 ctatcaagta tacttacgat atttgcatat tcaaatatta aatataataa taacaaattg
    19921 atatattcta atatgataga taaaaaaact caaaatagtg taaaggaaat cctaaaagaa
    19981 aataaaatta atgaaaaaga catagataca tttattaaat cagtcaacaa ttataataaa
    20041 ttgcaagtta aaatacttca aaataatata aatatatcta aatctggata ttcttcaatc
    20101 aatgcaaaac aagttccata taacctagaa aaattgcaag ataattgggt gaaaaaattc
    20161 cctgattata tggatgtaaa ttgtagaata actgctttta gactttttaa agattttata
    20221 aattcaaata agaaatttac tggagactct attgatttga atgtcgactt ggacactata
    20281 atgaataata aagatgctaa atttagcacc aaagatgtag aaaaatttat caacttcttt
    20341 tctgcaatac cagctaaaga tacagatgat acaatcaaaa ttgcagaaca aataaaaaat
    20401 gaatggaaaa aaagaaaaat atcttttaaa aataataaaa atatatctat tataaatgga
    20461 tttcttcgtt atccagaaac taaaaatgta tttataggtc atacaggcat atgtattaaa
    20521 actaaaaatg gcattctatt tttagaaaaa tacggtgtta catcacctta tcaagttact
    20581 aaatttaaaa ataaagaaga tgtaaaaaat tatatgttta atagattgaa gatgtcagaa
    20641 ggtgaaattg aattacctga ccctataata atggaaaacg ataaacttat gaaataataa
    20701 ctttaaacgc aaaatagtat ttataattac taagtcaata taatagagaa ggtctataga
    20761 tttgtgtaaa tacaagctta tctacaatct tatataagaa tgtttatata aataagatag
    20821 attaattcta agtcctatta ttctattgaa ttttattatt attttattta gattctattg
    20881 ttattttact tagaacttat tattattttg tttaagttct attactattt tcaatcatat
    20941 aaatttacaa aagactgtac ataaatttat tttatacaaa cttatctaca gtcttttgtg
    21001 tgaatatatc taaataatat cgtatttatt tatttcctct gcacagtgag aatcagaacc
    21061 taaaactaat ggaatatcgt atttttttac caagtctaat aagtatccat ctatataagg
    21121 ctctttgcag tactcttttc tatttccaga aacattataa tcaagttcat aacctttttc
    21181 tttaattaac tttattaact cttcaagaat tttatttcct ttataatcgt atggaaatac
    21241 ctgattaaac tttctaacta aatttaaatg acctattctt tttggtttat gtatacctaa
    21301 atctgaattt acagctttta tcaatgtttc ataatactta ttataaacag cttcaatact
    21361 tcctaatttg tcgattaaat tttgaaactc ctctacactg taatctacac aataataatc
    21421 atcatcaatc ttaattatat gtactgataa aagagaatcc tctaattcct ttccatattt
    21481 atttaaattc tcctttatcc cttcttcgta tccttctata aaatccactt ccgacccaac
    21541 atttatcttg attttaccct catacttttc tttcaacttc ttgacatcat taaaatactt
    21601 tggtaaatct tcaatactca tacaactatc attttcagga gcagggtctc taaatccatt
    21661 tgcatacgga aaatgttctg taaatgtcat ttctgttatt ccaacatcca ttgctctttg
    21721 tacatatttt tcaaaatcat ccttacttcc atgaggacaa taaggtgaat gtatatgacc
    21781 atctttcata aataattcac tcctttttta aagaaaaaat ctaatataaa tgcataaatc
    21841 ttttaacttc aaaataatat ttttaatcat gctaattttt attatatcta atattagagt
    21901 aattttaaac tgccttttaa gtatcttatc attagtacta ttttcaactt aacattaata
    21961 ttatttttta acttaacatt aatactatct ttaaatagta atactcaata tataattact
    22021 taacataaaa taactcaaat tgcttgatga ggcttttgca gtatttaaag aatatataat
    22081 tatttaacat taaataatcc aatcttgtaa taaaatttac tcattaaagt atattacaga
    22141 aaatacctca aaataaactt tttagttcat gatatactct tttttgcatg aaattagata
    22201 tatttttatc atttcaacaa tgaaagggag gcttatatcc atctttagac aaccccttag
    22261 ataaaatttt taataattaa acatcacaaa atataaaaat cgcttatatc aaacgtataa
    22321 gcgattaatt atatttacat atgtttttta gcctctaaca acattttatt agcttcttcc
    22381 atatactcta tagccttttc aatactcttt gctgtttctt cttttccaat agctctagcc
    22441 ttttcaaccc actctctaaa tccttcttca tgagtttcat tgtgatttat ccaatgtact
    22501 aaaagtattt ttaaagtttt ttcatcctta gactctgatg attcctcatg actatgttcg
    22561 tggtcgtggt cgtgagcatg gtcatgtgga tgttcatgac atatatctcc atgagtatgt
    22621 tcgtggcagt ggtcatggtc atgatgtcca tgatgatatc ttacaatctc tttatcaaaa
    22681 atcattagtt attccccctg ttattttata ttaaactctt tttttataag gtttacagaa
    22741 tcttttacaa gtagtgctaa tggtttacct tctaatccaa ctaactccac attttctggc
    22801 ataataggta aaagtatctt aagtgcctca ctgtctgcaa tagcttcact tatattacaa
    22861 gtaacttctc ccatcataga attaggtatt attacagaaa taggtgcaac tattatattt
    22921 gcttttttag ctgatactac tatagcattt tcccctgtag ctcctttatt agcatgagct
    22981 ttcatcatag aactagttgc tatagaatta gtccctagtg cataaatttc tacatatgta
    23041 ggtaactctt ctctcaaaga agaaactatc tgggctccaa tacctccacc cattccatct
    23101 atcactgcta ttatcatatg aaattcccct attaattaat tttcttgtat tattatctta
    23161 tggtccatca atcttatttc tttaattatc ccatctatta tcttttgctc tcctaataaa
    23221 tccgttaaat aaatctttcc atcacaaggg tctatattaa ccacattttc cataaccttt
    23281 tctaactcat tattactttt acaaatgaaa gctgatgatt cacacatata aatctcctcc
    23341 taatatgatt tttattaaaa atcaaaaaaa gattccctta ggtatttcaa tacaccaaag
    23401 aaaccttcat gatttcagtt tttgtatatt attatacctt aagtatatca ctttaattta
    23461 tgtaaagtca attaaagtta acgcttttta ttatactctt cattcttgta tttttcatat
    23521 agttctttaa ctaattgctt ctcataatca gtcaaatcgc cctccacaaa ctcaacatta
    23581 aattgttcag ctaatccctt tactatgttt ttctgtagaa tatctatgtc tatttttctg
    23641 ccaatttcat tttctattcc acttgctttt ttagctgtaa atttcatagc tctttctttc
    23701 aattctggtg tcttagtttt tattaattta aacaattttt ctacatcaaa atccagtatt
    23761 attgaaccat gttgtaataa aacaccatct tttctacttt gagcacttcc aattaccttc
    23821 ttattattaa tagtaacttc atatgatgca tgtgcattaa aacatgcagc tgataaattt
    23881 tccctactta ttctctcgcc tctatttaaa ttatctgttt ctataccact tagattcaac
    23941 cctttaacta gaccttcact tatatatcta taagataggt tgatgctctt atcgattaaa
    24001 ggattatctt ctcctataat tatactatag gttagctcat tatcatgtaa tacagctctt
    24061 ccacctgtaa ttcttctagt atagtctata ttcatacatc tacacttatc taaatctatc
    24121 tcatcttcca atttctgaaa atatcctata ctcaaacaag ctggttccca tgtataaaat
    24181 ctaagagttg gcttattatg tcctttctta tatgcagtaa atatagcttc gtcaattgcc
    24241 atattcattg ctccttcata tgatttatta tgtataactc tccactgatt catgattatt
    24301 acgcctccat ttttagtata aatatatcct agaaatcctc tatagattta tttctcttat
    24361 tgctaaatct atctcacttt gatttaaagg tcttgaatag ttgtatatag tacttcctgg
    24421 tctttcatta tagtatggtc tattacaatc cttacaacct cttatttcaa aaggatagcc
    24481 tttgtttatg tcttttaaaa tatcattgtc aagctttata ctatttaagt atccattttt
    24541 aaacttaaaa tactcttttg gataaccttt atttatcata taagacatta actgtaccct
    24601 tctataactt tctatgcttg gttgatttat cttttccatt ttagttcctc taactggagt
    24661 aaatgcaaat aaactaatcg ttacatcatt ttcctttaaa taagtatata aattgtatat
    24721 atcttcatga ctttcaccca taccaactat tatatgtgta cttattttat tgggatatga
    24781 ttttgacatt tcagttataa attttaactt ttcatcatag ttatttcctt ttattttttc
    24841 atatagttct ttatttgctg catctattgc tattcctatt ttatcaactc ctgctgaaaa
    24901 aaacttcttt atctgttcat cattttctaa ttttgcagat agtgaaattg gcatgtctat
    24961 cttaccactt atatagttta taaagtctaa aacagagtta tgtgcttctt cacttgccat
    25021 tgattgaata catattctct ttatattttt tcctttataa gcttttaaag catctaatat
    25081 ttcttctttt gaaaactctg gccaaattac tcttgataat ttatcttttc tagtactact
    25141 ttctattgat tgagaacaaa aactgcattt atttatgcat ttttctccaa tcattatata
    25201 tgctgtggta ggaggtatat cgcttttttt gtttaatatc ccaagttcta ttgctgtccc
    25261 aactgatagt ctaatcataa aacgcaacac tccttacact catttattgt catattcatt
    25321 tctatagcct tattctttgc agaccttgat ggtaatacta ttctattgac tccacacatt
    25381 atagataatt ggtctagctc ttttctatac actcctcttg gtctcataca acctagatta
    25441 atttcagtat ctggtaaatt aattctagct tcacataaaa tatctgctac cccttctaac
    25501 tcaggaggtt ctacattttc atactcagtc ccttttgttg gaataaggac tataaatgta
    25561 agcttattag gaggattttt ttgtaggtat tctataattt catattcacc ctttatttgt
    25621 cctcctttta aaccaataca tatatgtggt gctacttctg tatgttcttg tattgtatta
    25681 tagacttcaa tataatcttc tttactcttt ttcatcttat aaacttctct tattgtctca
    25741 tcatcaaaca ctaaatcaaa agaaactata tctaaatact tacacaactc tacaatgcta
    25801 tctttatcca taagacctaa atgagcattc aatctatatc cttgctcttt taagttttta
    25861 atggtattaa tatgagtatt tatagggaca tctccctcat aactacaacc gccactcaat
    25921 agaaagctag ttatattcct tgattgtact ttttcctcat attcttctat tggcaccata
    25981 ttgtttaagt agtgaccgtt gcaatgggca catttcaaac tacattgatt gcttgtagta
    26041 ctaagagcta gtgtctggtt aggataagca aactctattt tatttccaaa attctttaat
    26101 ttaatatcaa atgctatttt cattttttca gatagattct tattcatagt ttattccttc
    26161 ttaagtacat tttactcttc tagtaatttc tttgctgttg ggaactgctc tatattcgta
    26221 tgtggtaaga aacatgcaga aataaattca tccatataac ttggatatac actaagttca
    26281 acataagtta tttgattacc tatttcttca agtttatttt ttgcatcttt acttataagc
    26341 gctaagtaag aaccaacaag tgaactattc cctatataag taaatttttc tctatcaata
    26401 tcaggaagta accctataat tattgagttt tctatgtcta ggttgtttcc tataccacct
    26461 gctatataaa ccctatctaa tatactaaaa tccataccta aactatctac aagagtgtat
    26521 gcgcctgaat atatagctcc tttagctctg atgaaattat ctatatcaac ttcattgact
    26581 actatatcat tttctaaatc aaattcttct ttaaatgcta atacatactc tcctatctca
    26641 tgctcattaa atctaactct cttattatct aaatccctgt atattttacc tcttctatct
    26701 attacacctt tagtaatcat ttgacagatt aaatcaatta taccagaacc acatatacca
    26761 actggtgcac attcatcaat aatctttaat gttggctcta aagtatcttt atcaataatt
    26821 actttctcta ttgctcctgc tgatgctctc attccacagc ttatccctcc accttcaaaa
    26881 gcaggtcctg ctgaacatgc acagctcatc atataatctt tatttccaaa tacaatctct
    26941 ccattagttc ctaaatctat aaataaaaca ttttcttcac ttgaccatat ccctgctgat
    27001 aatacacctg ctgttatatc tcctcctaca taacttgcaa cagatggagc taagtacaca
    27061 tatgcactgt catttacaaa aagaccaaca ttttcaccca taagttttgg agattttaaa
    27121 aatggtggta tgtaaggttc ttgtcttaag aagtctgtat acacacccaa gaataaactt
    27181 gtcatagttg tatttccggc agccactaat gtaactacat cttccttatc tattccattt
    27241 ctttcatata ttgtttttaa tagtggattt atagtttcct caactatagc tttatgaagt
    27301 gtctctaatc catttttctt agttgaatat ataattctat ttataacatc tgctccatat
    27361 tttatctgtg catttccaga agatgcttta tctactacct cttttgagta taggtctact
    27421 aaacaaacaa ctactgaagt tgtaccaata tctattgcaa ctccgtataa ggaattttct
    27481 ttatttcctt gttctatatt tatgattgta agtttctttt gtttttgaac atatgttatt
    27541 gtaaccttaa aatcactctt tctaaataca gttggcattt ttctaagtat atccaatcta
    27601 aaatctattt cattatatcc taggttattt ctaacatatc tttccaatct atctacatca
    27661 cttatattat catctaaatt tggttcttcc atttctatgt atttcttttt tatatttgtt
    27721 ttaaattgaa ggttatgttc ttcaattatt tttttagctc tttcaaaaat ttccctatcc
    27781 tcttttttat tactaccttc tatcttcata ccatgcatag aagatgatac tttagaagga
    27841 acttcaattt ctatatctga tattaccttt gtacaacaag ctagtatata accttgctcc
    27901 cactcatcat ctgttatatg tcttgttttt tctgtatcaa cttttccatt caataatttt
    27961 actttacact tcccacaaga cacgtttcca ttacaaggag catctatgaa tatatctgca
    28021 tttcttgcaa cctctagtaa tatatccccc tcattacaat aaacctcttt attgtttggt
    28081 gtaaaactta cttttatcat gttatccctc ccaatttgtt ctatatatga taaaaggaaa
    28141 gtatagctaa atttgcgtgc actcaaattt tactatactt tccccaatta taataatatc
    28201 ttaatcttta attattctaa tccagcctcg tattctttat cacttaataa cgtatcaagt
    28261 tcagccacat cagctagttt tacttttaat atccagtttt catatgggtc ttcatttata
    28321 cattctggct catcttctaa tttttcatta acttccaata cctcaccatt aacaggagat
    28381 attaaatcag aagctacttt agaagactca actactccaa aatctactcc cttagttaca
    28441 gtatcttcta cttctggtgt ttcaacaaac aatatttctc ctaattgatc ttgagcatag
    28501 tctgttatac ctatatatac aacatctcca tcaattactt ttacccactc atgatcttta
    28561 gagtatttta attctggtaa taatttcatt tcgatagacc cccaatatta tattattgat
    28621 taaattactt aaatcatatt aatattatat catattattt taaattttcc atcaattaca
    28681 ttacactttc catagctaat gctggatgac ctttttcttc taagaaagct aatactcctt
    28741 cagaatcgtc agctatagtt tcatcacaaa ccatatcaca gaagttttct attccagtca
    28801 tttctttaac tgtagcattt aatttatcag ctacataatc ttttaattct tttggcatcc
    28861 aaactattct ttctggtcca ccttctgcat aagcgaattt tttagaagat ataaaatgtc
    28921 ttccgtgtcc cataaatcct ggagtttgaa ctcctccacc tgtcatagaa gcaagttctc
    28981 cgaaagtcat accaactgga gtaacacttg caaattctct gttaactaca acaaatccat
    29041 ttgcttctgg cattatacca cagatacatt cgaagcatcc acaagaagtc attgggtctt
    29101 ctagtataga gtataatgta acagattcaa ctgcaccttg agatatttgg tttacagttt
    29161 catttactga attccataca cctgttctat catctaagca ctcgcctttt tctattggtt
    29221 gacaaggacc tgttgggtct aattctttag tagccttagc atctaaccag ctaactgctc
    29281 cacaaagacc aagtctttca ggagtaacaa cacaaacgtg agctggtgca aatgattgac
    29341 ataagttaca agaatagaaa gtatcaacac tttcatcaac taatgaagct aatctttcat
    29401 ctctagcatt gtacttagct attgcttctc cttttaattc agaaactttt tcagcatctg
    29461 taattatagt tatttcacac ttatcaacta ctgattcaaa ttcatctaac atcttagcat
    29521 ataaaacttc tcctatatgc tcaagtctaa atcctttatc aaatgcatct ttagagattc
    29581 ttacccaagc catatctctt tgtccaacgt gcattacacc ttctatatag tttaagaagt
    29641 agtggaaacg tctttcaaga actggctcga aatcttcttg catattttta ccagctattt
    29701 taactattac agcaagtggt aatcttaata caccatctgc atcaacttca tctatatttg
    29761 gccctattat ttctatctta tggtcttcaa cttctgttgc ttctttctta acaacaagct
    29821 cccaagcttc agttctgttt ccaccaaact cagcaaacat atcgctcttt ctaattctct
    29881 caccttcaaa tgctgctgca aatgaaactg ggataggtat ttcagtaact tttattttta
    29941 ttcctctagc ttctaatgaa gtagctacta tcttatcgta atctttttga gttaataggt
    30001 tcattggaac ttcaagaact gtttggtcag ttataactgg gaatcctaaa gctattgctc
    30061 cagcacctgc agatacaact aattcactta atggtccaaa tgcattaaca aatgcaggaa
    30121 ctctattagc tgtgtactct aataatccgt ttaaatcacc aggagttaat ccaccgaata
    30181 ttaatgctgc tcttactgca acagatacaa cgtgtataac agaagttaca tcatatccta
    30241 gaggtataac tcttagctca agacccattt ttactccagc ttctatagct tggtctataa
    30301 ctttaccaac taagaaagtt agtaaacctt tagattggta atcttttata acttttgctg
    30361 ctgattctgc atctggacac tcaccaagaa caactgcaac accaggtata tctcctgtaa
    30421 ctaatggtac accaagtgat ctgattatag ggtcagttat atgacctgca catggctcac
    30481 tatatggagc atccatagtt gaatatttta atgcttcaat tacttctgct gctaaagctg
    30541 tagctaaacc agcattaaaa gcatgttcta gtaaatgagt tctatttatt aaagatttta
    30601 ctacttctaa agcaccttct aagtccccta aagtgttcat cttttgtcct gttgcagcat
    30661 atatacaagg taatgaatat gctgtatcag ggaaagcaac tttatgctct tttccattct
    30721 tttctatagc ttcagctaac atagcttgag ccgcacctaa agcttgttct gaccctgtaa
    30781 agattatatt atatagattc attctatttc cccctttaat atttacacct attttttatt
    30841 aactataccc taaaaaccct agaaagcaaa tccaccttca tttaactcta agaaagagtc
    30901 tgcatataaa gatgccccgt tgattatgtc acaagatttt acagttttca ttaattctgg
    30961 gtcacatgga ttcattatag ctgaactaaa tccattagcc attgccattg ctaagaatgc
    31021 actatctaaa acaggtctaa catgcttagg acatccatta gatacgttag ataatcctcc
    31081 tgtagttaaa agtcccatct cagtcatcat ttttatagct tctaaaactt ctacttgttt
    31141 ttcttgcata ccttttataa ctaagcataa tgggtcaaat agtatatcag ttggctccat
    31201 tcctaacatt aagccttttt ctaatatttc ttggcagtaa gccatacgct catcattatc
    31261 tcttgggata ccttcttttg cacataaacc tataaccatt gcttcgtatt cagctgctat
    31321 atctattaaa tcaaatcttg agccagcatc agcagagttt attattggtt ttgcattagt
    31381 tctatcataa actttaagtc cagcttcgat agctttttta ttagctgtat ctaatgctat
    31441 aggaacattg ttgaactcag attgaagtaa tttgattccc catgtcatta tttcttcgcc
    31501 atctctttcg gcaggtccta tattaaaatc tatatagtgt gctcctgctt ctaattgctc
    31561 ttttgctctt tttaagattg gagctgggtc tctttctgct aatgcttttc ttattgaagg
    31621 tgatatacaa tgtattctct cacctataat cataaatttt tccatttata atgtccccct
    31681 ttaaatttaa tattaatagt tagttaatat tttattagtt tgtttggcat gctttttctt
    31741 ggtatccttt taagaactta ggtaattcca tagattcctc tggtcctata actacattcc
    31801 atcctggtaa gttatcttct atatcacctt taagtactgc aacttttcct ggaatgataa
    31861 ggtctttaca attagttact tcttctacct tactttcttt aatgaaagca gagattgagt
    31921 ttccaccaaa tttaccagca gcccaagatg taagaactga atatccacct gcatctggta
    31981 ttactaacca tactggaact tttgatcttt ctatatcacc agcaactatg aagtaagtta
    32041 atgcaaagtc aacagtaact aatactggag aattttcatc tggattgttg attggataaa
    32101 tctttggttc aactctcatt ggtctttgtg ggtcagtgta tatgttttgt cttagtgcaa
    32161 ataatggtaa tgccttagca taactaatat catctattac tattatagaa ccgtatttta
    32221 tagtgaatat tgatgataaa gcaacttcca tcatagggtt tgaattagat aatctatttg
    32281 caaatactat tgaaggatat ccaaatgttc tatcttgctc ttttaaagct gttcttctaa
    32341 cttgtatagc attagtgtaa gtatctttta tgttttctcc tgttacatct aatactaatt
    32401 ctttatatcc agcggcttga attaattcaa ctgtttcata taattcttct aagctaccag
    32461 cttttacacc taatggtaaa cttgctcctt taactacttc tatcatatct ttgtagttct
    32521 ctttagttgc tccatatacc attggtctag catcttttaa tacttcaaca gcttctttaa
    32581 ctacttgtgc atcatcacaa gctaaaatat aagctacttc taatccactg ccttttattt
    32641 tatctattaa ttttaaatac gcatctttat ctccacaata ctctaaaaca gccatttcaa
    32701 ctttcatttg ttcaccaatt cttacatagt caactttttt catgttagct attttagcat
    32761 ctactgcttc atcactcata caagtacaga atgaaactgc atatctattt ctacttacta
    32821 atgttttttc atgtctaaat aatactgttt ctccacctag ttcatactca gatgcaccag
    32881 caccaacttt taaagctttc attaaaggtg cagtagcctc tgataatttt gctatagcgt
    32941 catcagacat atgtgggcat ttacctactt ctacagctcc tgaagcaacc ttcatagaga
    33001 aagccataca agttgggaat ccacaatcct tacagttttt ctttggtgtt aatttaaata
    33061 tatctaaagc ttttaatgcc attatttttt ccccctaacc ttaacaattt aattaagcta
    33121 atgcgttaac caattctttt atagtttcta ttgattttgg atgacgaagt ataactgcgt
    33181 ttgaaccacc tactaaaaca cttgctgcag ttgaaacttc cattgctata gttctttctt
    33241 ctggacaacc ccaatctggc tcatcttcta ttggagctat agcttcttta acatgaccta
    33301 cttcaaaagc tactggtgtt atgataggca tttgtaatgt cttatcattt tgaccaaatg
    33361 ctgcaagtct aattctatcc atagtagatg caacgtactc atatccataa ccaactgctg
    33421 aacatccaac attcataact atattttcac ctttaacgcc tagttgagtt aacaatacgt
    33481 ttaattgttt agctaggttt atatcaacag aagattcagc tcctacttta tgtgcataag
    33541 ccataccagc tgatgctcct actcctttgt agttatcttc tactgctgac ataaataagc
    33601 agttatgtcc atccaatgtt tgagctaatt tttcaaataa tttaccatct ttttcatgat
    33661 ttcctgaacc agctacaact aaaggtaatt ttattgcttc aattactgcc ttagctacat
    33721 cagcacattc atcaactgac ttatctaatc catttgggtc agaaccatca aattttaaac
    33781 aaataaaatc tgcttgtgta ttagcttcaa catatttagc ccactctact ggacaattag
    33841 ctacatcttt gtataattcc ttatatgaat cagtccagct ttcaggataa acgtctgata
    33901 tttgtatacc tatttttgga gaattaccta cttctccatc aaaactataa aaaggtaata
    33961 cattttcccc acctaattta attgcttttt cccctattcc tacttcaact tctgatattt
    34021 ttccagaata tttttgagta gacattttaa atgccatatt tttttccccc ttaagcctat
    34081 tttaattcta atttagcaaa aatttctttc atagccactt tagattttga atcttcaggt
    34141 aaattaacta aaggaatacc tgtagaatca tattcatata tcaattcatc cataggtaca
    34201 acaccgatta gctctaggtt atgcttttca atttcttctt taacaccatc attcataacc
    34261 ccatttggaa ctttatttac aatcaaaagt attcttccaa cacttaactt tagttcctca
    34321 gctaaatctc ttattctagc aacagcttgt atgctacgtc ttgagcaatc actaaccaat
    34381 aaaagtgtat caacatgtct agtgactttt ctacttagat gttccatacc agcttcgttg
    34441 tccatgacta agtatttgta atgaccagat attttgttta cttgttctct aagtatccca
    34501 tttacaaaac agtaacatcc ttcaccttct gacctaccca ttactaataa gtcatatcct
    34561 tcgccttctt cgattataga atttaatcta aattgaagat attgtgcctt tgtcatacca
    34621 ccagggaatg cattgcctaa cttttctctt tgatttactt cttctcttat ttctcctatt
    34681 gttgcttcaa cttcgatacc taacacttca tttatgttag cattggcatc agcatctaca
    34741 actaatactg gtcccttttt gtccttaacc aaataatcaa tcaaaagccc tgtgagactt
    34801 gttttaccag ttccaccttt tcctgcaaca gctatattgt atcccatttt aagacttccc
    34861 ccttaaatct agccacagag ccttatgtac tctgtctgtc tattataatt taggtacaga
    34921 atgaacacat tctccatgta catcatgaag tgcttccatt agcccttcag ataaacttgg
    34981 atgtggatgt attgcatccc ctacttgttc tactgttaat cctagatgaa cagcaagtga
    35041 caactctgtc aataaatctg tagcatgagg tcctactact gctgcaccta ttatcttatc
    35101 agtttctttg tcagctataa cttttacaaa tccttggaaa tgtcctatag cttgtgcttt
    35161 tccaagacct ctaaaatcaa attgacctac attgtattct acaccttcag cttcaagttg
    35221 cttttcagtc ttaccaacac cagcaacttc aggttcagta taaacacatc ttggaattgc
    35281 tctataatct actaccttag tcttacctaa agcattttca actgctacga taccttcttt
    35341 agaagcaaca tgtgctagga aaggagtatc tattatatcg cctattgcgt atataccttc
    35401 tacattagtt tcaagatgtt cattaactac tacttttcct ctttccattt caattcctat
    35461 atcttctact ccagaattgt caaggttagg tcttctacca acacatacta atgcatattg
    35521 tgcttctata acctttccat tagaaagagt tgcaacagcc ttaccatcaa ctacttcaca
    35581 agtttgtact ccaatacctg taataacctt aattttatct ttcttaaatt gtctaagtag
    35641 ttgtttagct acatctttgt cttcatttaa tagtatttgg tcaaccatct caactatagt
    35701 tacttctgtt cctaaggctc taaagaactg ccctatctca catcctataa ctccaccacc
    35761 tactattaac atagactcag gtatctcttc aagtccaaga acttcatcac tagttattac
    35821 aacttttcca tcatatggga acattcttgg tacaactgga acagaaccat ttgctagtat
    35881 tatcttatct gcttttatag tttctactgt tccatcatct ttagttactt ctatagtgtt
    35941 tttatcaatt aattttccaa aaccattaac taagtttact cctctttttt caaataagaa
    36001 ctctatacca cttattaatt ggtttacaac tttatttttt ctttccatta tggcagtgaa
    36061 gtttggctta attgtaccat ctatttctat accaaaatcc tttgcttctt ttacagtatt
    36121 caatactcct gaagaagcaa gaagtgcttt agttggtata catcctgcat ttaagcaagt
    36181 tcctccaact cttctttttt caacaactgt tacatctgca ccaagcatag aagcttttat
    36241 agctgctaca tatcctcctg gtccaccacc tactactact atcttcatct ttaattcccc
    36301 caaacctaat ataatctaca atcttgcaat actataaacc tgcggcatcc agtatagcag
    36361 gtatattctc ttctgcccca attgccatta tatgaacacc atcacataaa tcttcttctt
    36421 ttaattgttt gataagttcc cctgccattt ttattccttc acttacccag ttttcttttc
    36481 cagctgctct taatctttcg atttgttcat ctggaacaaa tattcctggt acatttgcag
    36541 tcataaattt agccattcct ggtgatttta aaggtactat tcctgctaat accttgcaat
    36601 ccatatccct tgttaattct ctaaactttc tcattgtatt tatgtcataa agtgcttgtg
    36661 tttggaaaaa tttagcccct gcttttattt tctttttcat ttttaataat tgaacttcta
    36721 ttggagaata ttctggagtt actgatgctc ctagataaaa atctggagaa ccttttaact
    36781 tattaccagc catgtccgta cctgccatta aagtctcagc agtttgaagt atccccacag
    36841 aatctaaatc aaacactccc ttagcttgtg gatggtctcc tacgctagta tggtctcctg
    36901 ttagtgccaa cagattgttt atcccaaata cacctgctga caacatttct ccttgtatag
    36961 ctattctatt tctatctctc cctgttattt gaataactgg ctctaatcca gcatctttta
    37021 ataatttaca tgtagcaaga gaagttgctc tcataactgc cgattgaaag tctgttacat
    37081 ttgctgcatg aactcttcca actaacggct ttgcacattc tattaaatgt gaaaggtcag
    37141 tcccttttgg aggagccatt tcagtagtaa ctgcaaattt tccactttct aatgtttctc
    37201 ttaataagct catctctaat ccccctttta accttctaaa aaaactttta tgaatttgct
    37261 gctgcttctt tctttttctt tgcacttaaa cttcttggat tattttgctt agaatagtct
    37321 tttggtggtc ttatttctgc taagttatct aattgtccta tagcttctag tctttcatat
    37381 atctttatcc aagcacaatc attctctgag tttacttcac acttaccatt tttagctcca
    37441 ccacaagccc cattcataag acctttagca cacatagtta ctggacatat cccacctgtc
    37501 caaccaagtt cacagtctcc acaagcctta caggcttctt catattctcc aactctttgt
    37561 acttccccta taaacaaagt attgttggct ggataaactg gcttatcttt taaattctta
    37621 actattgtct gtgtaccatc tccacatgct aaagataaaa ctgcatcagc ttccttagtc
    37681 tcttctttta gagcctttaa atccttttta gactttaaaa ggttacaagc agggtcaagc
    37741 attacatatc ctaagatttt tttgccttca ccttcaagag tttccttcat ttttaaaact
    37801 tcttcttccc caccactttt acaagttgct gcacattgat tacaaccaac taaaaccagc
    37861 ttgtcaaaat cttttaagta ccctagcact tcttctaatg gtttattttc agaaattatc
    37921 atatctactt cccccctaaa ccttccacat atatatatct atttattgtt ttgtaatttg
    37981 caagccttaa ctacatgttt tgcaagtata gatgttgtaa ctgaaccaac ccccgctgga
    38041 actggagtta tcattgatac tttgtctaaa actgcatttg tatcaacatc tccacataaa
    38101 tttccttctt catcaacatt tataccaacg tctataacta ccgctccatc tttaacaaaa
    38161 ctttcatcaa ccatcttagc tctaccaata gctgctatta gcacgtctgc ctctgctgct
    38221 acacctgata gatttttagt ttttgaatga catattgtaa cagttgcgtg ttcacttaat
    38281 aaaagcattg atactggctt tccaactacc attgatcttc caagaactgt taccttactt
    38341 ccttttaaat caacattata gtgttttaat atctcaacta ctgctgtagg tgtacatggt
    38401 ggaaatccac ttttatcccc ttccattacc ttagcagagt ttattggact aaaacaatct
    38461 acgtcttttt ccggtgcaat aacatatttt ataacttctt catttaagtg ttttggaaga
    38521 ggtctaaaac ataatatacc attaacattt ttgtcatcat taactctctt taatacatca
    38581 atatattcct cttgtgttat atcctcagct aactctaaca cttctgttgt tatacctatg
    38641 ttttgacatc tctttagtgc tcctctttca taagataaat catcacttct tgccccaact
    38701 ctaactattg ttagtttagg atttatacct tctttaatta gtaaatcgac ttcttttatt
    38761 aattcttcac taatcttatc agctacaggt tttcctttta taatttgtcc tttagttgac
    38821 attccttcca tacattcaca actcccctac aagcaaactt aaattaattt attctatata
    38881 ccacatatta ataacatagt atctactttc gtcatttaaa aattattttc caagagcttt
    38941 ttcaactttg gcataaactt catcacaaat ttttacacca tcttcaacta acttatctac
    39001 ttctgttttt accttattaa catattcttt atcttgtata gaatttatat ttataactac
    39061 atttaattgt ccaccaagta tagcagctct taaacactga actcctacac caacatcact
    39121 tatagcaagt cttgaacctt tatcaactaa atcttcatgt aacttaatag cttcatagca
    39181 aactcttact atgtttaatg gtacttcaca agcaactttt aatgcctttt ccattgtttc
    39241 tgcttttatt cttttttctt cttctgtttc tttaggaagc ccgtaagctt ttgataatgg
    39301 taagaaacat tctgcatcat catctatcat ttttaataaa tctttttcta actttcctgc
    39361 cttacttaat atctctttta cactttcttc atactcagca tacttcttct tccctattgt
    39421 aaggttacat accatactac ctaaagccat acctattgca cctactagtg cagcagctcc
    39481 cccaccacct ggtactgctt ctttggaaga tagtacttct acaaaatcaa cacaagtttt
    39541 atctgctatt ttcatgataa tcctccttga aaggatgtag gaaaatatct caaattgttc
    39601 gagatatttt acgaactatt ttaagatatc tcctactctt tatttataaa ttaatttcct
    39661 tagaataatc cagctattac tccattttca tcaacatcta ttttttctgc tgctggaact
    39721 tttggtaatc ctggcatctt cattatttct ccagtaagtg caactataaa tccagctcct
    39781 gctgaaattg ttgcttgtct tacagttatt ctaaatcctg ttggtctacc taatttagtt
    39841 tggtcatcag ttaaagaata ttgagtctta gccatacata ctggaacttt agtaaatcca
    39901 agtttttcta agttagctat ttccttatca gcttcaggag tataatctac accatctgct
    39961 ccataaatct tagtagctat agcatttatc ttgtctttta tagataaatc atcttcatag
    40021 cagaatctaa agttattttc ttcttcatcc aataatctta aaacttcttt agctactgca
    40081 attccacctt ctccaccttt tgcccatact tcagaaagag caacattaac acctaattcc
    40141 ttacacttgt cttcaacaag tttaagttca gcttcagtat caagtgggaa tctgttgatt
    40201 gcaacaactg ctggtaatcc atatacttga gttatgtttt caacatgttt taataagttt
    40261 ggaagtcctt tttcaagagc ttctaggttt tcgttattta agtctgcttt agcaactcca
    40321 ccattgtatt ttaatgctct tacagtagca actattataa cagcatctgg ttttaagttt
    40381 gccattctac attttatatc taagaatttt tctgctccaa ggtcagcacc gaaaccagcc
    40441 tcagttatta cataatctgc aaagtgcata gccattcttg tagctataac agagttacat
    40501 ccatgagcta tatttgcgaa tggtccaccg tgtacaaatg atggtgttcc ttcaagagtt
    40561 tgaactaggt ttggttttaa agcatctttt aataatgcag ccatagcacc attagctttt
    40621 aattgtctag ctgttacagg ttcaccttcg tagctgtatc caactattat gtttcctaat
    40681 ctttctttta aatcagatat atcacttgcc aaacagaacg ctgccattat ttcagaagca
    40741 acagttatat cgaatccatc ttgtctaact gctccatcac cttttttacc cataccatca
    40801 acgatatttc taagttgtct atcgttcatg tctacacatc ttctccaagt tattttctta
    40861 gggtctattc taagtgcatt tccttggtga atatggttat caagcatagc agctaaaaga
    40921 ttgttagcag ctcctatagc gtgaaaatct cctgtgaagt gtaagtttat atcttccata
    40981 ggaacaactt gtgcatatcc tccaccagct gctccacctt taacaccaaa tactggtcct
    41041 agagatggtt ctcttaaagc aactaataca tttttgtcta gtttagcaaa tgcatcagca
    41101 acacctatag tagtagtagt ttttccttca cctgctggag ttgggtttat agctgtagtt
    41161 agtattaact tagctttttt accagtctct ctttttagta gattgtagtc aacttttgct
    41221 ttgtatttac cataaagttc tacatcatct tcacctagtc ctaatttttt tgcaacctct
    41281 ctaatatctt gaggcttagc ttcttgagct atttcaatgt cagatttaaa tcccataact
    41341 gaatctcctc ccacaaccta aatagattta aaatataatt tttgtaaaaa ttgtattaat
    41401 atgtaaaaat tatattttat aagataatta tacatgtaaa attacttaaa aactagctac
    41461 taagctatta aaatattcaa acttaatagc tagttattta tctaattact tacttattac
    41521 ctatctattg cctatgtcta acctttaacc aacttaaaat cccaaatcaa aatttctatc
    41581 tattttattt atccttaagt gacattaatt tatcttttat tgctttaatc tgttctttcg
    41641 ttgtttcact agaatcatat ggagattgat tagctctatc agaatcaata acatcttggt
    41701 tataataaat aaatcctaaa gattctccat cttctaagtt ttggattata aattcttcat
    41761 cttccttatt tctaatttta tttcctacta caaatacttt atttacacct atatcatgtc
    41821 caagtttctt aacttttcta taagtctgta gacttctctc tcctggttct acaactacta
    41881 taaaagcatc tacaccttgg gctgttcctc ttcctaagtg ttcaattccc gcttccatat
    41941 ccataaccac tacatcttta ttttgtagta ttaaatgtga gcagagtctc ttcaaaagaa
    42001 catgctctgg acatacacat ccagtaccac cagaatctac ggttcccaat gtaagtaatc
    42061 ttactccatt gtattcttta cagaaatttt cagggatatc gtctacttta ggattcatct
    42121 taaacatttt tccaaatgaa cctacagaag cagctgttct atctgaaact aattttttca
    42181 tttctgatat aggtactatt gattcatata cttcttttgg aaaacctagt gctaaagcta
    42241 aattagcatc agggtcggca tcaacagcaa caactctgta tccgtcttca gcaaacatcc
    42301 tagagagcat tgaagaaaaa gtcgttttac ctactccacc tttacctgtt attgctatct
    42361 tcataatccc taccaccttt tattactatt tgctttttag tagttaattt tacagttaaa
    42421 tattttttat actctatatt tttatacact aaaaataaac tacagctagt ccttacagga
    42481 accaattaaa tttaccctac tagcttttca aagcagcatg tatagtttta ttattcttta
    42541 atattgttta tattttattt attcagtata ttcattatat tttgatgttc tttactttaa
    42601 tatttgactt tattcaactt tattcaacca gaaacttctt ataaaaacgt ttattctata
    42661 acttcactat tttttaagct tcagcctttt ctgccatctt ttcttcaact aatgcttcaa
    42721 agtgaacacg ttttttctct atatcttcta ttattgtagc tgctaactct tttggatttg
    42781 tatttacagt aaatgtagct ccaactttat cttttaattc actagttaat atatccactg
    42841 ctcttgaaga accagctatt ggaggcatta ttcctaaata agtgtctata ccagaagcta
    42901 caacatagca tcctatagca actgcttttt ctgacatcca ctctggagct atacctacaa
    42961 ctggaaggtc acacatatcc atatctaagt aattagctgc tgctccaact aaatctagta
    43021 tacgacttat atcaacacaa gaacccatat gtagtactgg tgggattcct actaatttac
    43081 agacagttgc aagaccttta cctgcatatt tttcagccgc atcagattcc attaaaccaa
    43141 acttagctgc tgctgaagct ccacaacctg tagtaactac tattatgtcg tttttgatta
    43201 attctttcat tattgttacg tgagcctcat ttgaaactcc tttagcattg ttacatccaa
    43261 ctacaccagc tgctcctctt aatactccag attttaaaca gtctgttaat ggcttaactg
    43321 ttcctgctgg gtctatatga gagtttacaa ctctgtctaa ttgatttact actgcttcta
    43381 cactataccc aactgttgcg cctgatttta attcaggtat aagtactttt gatttatctc
    43441 tatttttaaa gtttaaaata gcttctttaa ctattgcttt agcatcatca agtgcttgtt
    43501 cttctctaaa ttcaatataa gttgaacctg ttatcttagc ttttggagaa gttgttacaa
    43561 atttagtatg gtaacaatct gcaactctag ctaatgcagg gaatatacat tgaacgtcaa
    43621 ctattacagc ttcaactgct ccagttatta ttgcaagttc ttgttgatgg aagtctcctg
    43681 ctatttttac accatgtctc attgtaatct cattacctgt acaacacata cctgctaagt
    43741 ttattccatc agcaccaact tctttagcta aagctactaa gtctggctct tctgatgcta
    43801 aaactatcat ttctgataat gctggctcat gtccatgtaa tattatgtta actttatttt
    43861 cttctaatac tgctaaattc gcctctgttc ttcttggcac tggagttcca aataagatat
    43921 cgcttagacg agtacctatc atagaaccac cccaaccatc agccatagaa gttctaagac
    43981 tcatgtgtat taaactatct atatcaccag tacatcctat atgagtagag tgcattattg
    44041 ttgcaatttc tctatctata gctcttggct ctatagcgta ttctttccat attttttgtc
    44101 ttggttctgg tgcatttttt ataaatctta atgtcccaaa aggttttcca aattccatta
    44161 atgcaatttc tgccacttca tgtgctacat catagatatc tctaccttct gtctctacat
    44221 cccattcttt agctaaagta attaacttag cttcgtctct tattttatag tttccatttg
    44281 gatcagctaa tgctaatgta tgtgctatat ctctagcatg gtctgaatgg gcagctgtcc
    44341 caccagcaac cattcttgca aagtttctac ctactatagt atgctcatct gctccacata
    44401 acccccttgg agtcttaggg cttattctac atggtcccat tccacatatt ctacagcaaa
    44461 caccttgaag accaaaacca cattgtgcct taaagccttc ttttctatca tacattgttt
    44521 ctacgccttc ttctctagct ttagctatca tcttctggct gtttaaatca attgttaaca
    44581 ttttttcatc catattccta tccccttttt ttgattttta aaatccccca ggtgactgac
    44641 ttcatgtcat aaaataataa aataaatgct ttctaagttg attatatact aattttcagt
    44701 gctttgcttt agatttaaat atttttttaa caaaattaga taatacataa aaattttatt
    44761 ttctacactt aatttaagtt gattttatcg cttgtttttg gctatttgtt aatatctaaa
    44821 cctacttttt tgatacttca gtttaattaa attatgtttt gattttttta aatcaaattt
    44881 gaaatttttc tagtcatttt tatattattg cagattgttt gtgtatttat tatctttcac
    44941 tactgtttgg acttattaca gatttttact ccctaaactt aattagtaaa aacttcttag
    45001 aatagcattt tttggactaa tttttttgaa tttttttatt tttcctactt taatattaat
    45061 tatagcttca aattgaacat ttgtatatat tcatttgaag ctataattat tttttatata
    45121 aaactttttt aatacagcat taatttagac attttgacta ttttttctac tttaattcat
    45181 ttatttcaag cgatttatcc tatttcatat ttttagctac aatagcccca tatgcaatag
    45241 aatataattt agattcatgt gaatcagctc ttgacactaa aactatagga gcttttgcac
    45301 ccataattat acctgctgat ttagagtctg ccatatatgt aaaggctttc cctattccat
    45361 taccaacctc tattgttgga accaatatga tatctacatc tccagatact tcacttttaa
    45421 acccttttat ctcactagct tcttttgaaa cagctaaatc aaaagccaga ggaccttcta
    45481 caattacatt ttcaccaaat tctccttctt tacaagcttt tgctaacata tctgcatcta
    45541 cagttgcttg catcttagga tttactttct ctttagctgc caaacatgca acttttacag
    45601 tttccattcc taaagctttt gcagctttta tagaattttt taatatcttt gccttttgtt
    45661 cataatcagg agctatattc atacctccat ctgtagttat aagtaattta tggtattttt
    45721 ctaactcata taccataaca tgacttaata aactatcagt tctaagacca tagtctttat
    45781 ttaacacttc ttttaatatg acagatgtat ctaataatcc tttcattaca aagtcggctt
    45841 ctttacttga tactaattta actgctattt ctgcacactc tttgatatca tccttatcca
    45901 ctattttgat accacttaaa tcaaatccta tttcttttga tatttctctt attttgcctt
    45961 catttccaac caatatagga gtaattatat ttctttctac agcatccttt attgccatta
    46021 aaacttcttt atcatgagct gctgctacag agagtacaat tttctctcct cccttaagtt
    46081 gttctaaaat atcatctaat ttttttatca aaagtaaccc ctcccttata tatatttaac
    46141 taaataatac catatttgct ataccttttc tcaatttatt ttttatgtta taaagaattt
    46201 atataactaa aagacagttt aatattttat tctgggattt catcaataac tcttaaaagc
    46261 tttgcaggaa caccacctac aatagtattt tctgcaacat tttttgttac aactgctcca
    46321 gcagcgataa ttgcattatc tccaatagta actcctggaa ctacagttac attagcacca
    46381 atccatacat tttttcctat tgtgattgga gaaggataag ttgtaccacg tttctctggt
    46441 gcaataccat ggttaagtgt agccaatacc acattatgcc caatttgcgt attatctcca
    46501 atctcaatcc ccccctggtc ttgaaaatgg caactgctat ttataaaaac attttttcct
    46561 attgtgatgt tttttccaca atctgtataa aatggtggaa acataaaaaa tgattcatct
    46621 accttttttc caattaactc agaaaataaa ctacggactt cttctggttc atgataagaa
    46681 gaatttaact tacttgtgat tttcattgct tcattactca acttaaccat aaattcatgt
    46741 atttctgatt tactagaaac aggttttctt tgattgatat gtgttaaaaa ttcattgata
    46801 ttcattttat acatcctttc aatatgtgct ttaatttatt tgtacattct aaataatatt
    46861 ttaataaaaa ttaatttaat ttatttttat taaaatatca ttgcttcttt tagtgtaata
    46921 tacaattata tctatatcaa atatctatag taaatactta actatactta aaaagcatat
    46981 aaattgatat aattatataa tagataagtt aatattgagg agggataatt atggaaataa
    47041 gagtattaag atatttttta gcaattgcaa gagaagaaag tatatctggt gcggcaacct
    47101 ttttacatat aacacaacca acactttcta gacaaattat ggaacttgaa gaagagttgg
    47161 gagttaaatt attggttcga ggaaatagaa aaatcacctt aacaaatgaa ggtaggcttt
    47221 tacataaaag agcagaagaa attatatcat tagttgataa ggttgaatta gaatttgaca
    47281 atcccaatga gattattagt ggtgatatat atataggaag tggtgaaact gaagctatgc
    47341 gacttatagc tagaactgcc catcattttc atcaaaaata ccccaatgta agatatcacc
    47401 tttttagtgg gaatgctgat gatgtgactg aaaaattaga caagggactg cttgattttg
    47461 gtattttaat tgaacctgca aatatacaga agtatgatta tgcacaaatt cctgctactg
    47521 atgtatgggg tttactaatg cgtaaagata gtgcccttgc atcacaaagc acaataaatc
    47581 caaaagattt atgcaatata cctcttatta tttcaagaca gacacttgtt gataaagcaa
    47641 tttctaaatg gcttcaagac agtaatgaaa aactaaacat tattgcaaca tacaatttag
    47701 tttataacgc ttctcttttg gtagaagaag gtctgggcta cgctctgtgt ttagatagac
    47761 tcgtcaatac tacagaaaac agtaatcttt gttttagacc tctatctcct cctttagaag
    47821 ctaaattaaa tattatatgg aaaaaacatc aaatattttc aaaggcagca gaaaaatttt
    47881 tacttcaaat acaaaaagat ttttcttcta tcatgttgga tacatctaag ttggatacac
    47941 ctaaagaaaa ataaatcata ataaaaggtg agagtgtctc aaaatgaact ttgcactgta
    48001 taaggcactc ttttttatac aaagtaaaat atatgttcat catttcagca ctaaaaaaga
    48061 ggcttatctc tattttgaga tagccccact taaccaataa aattaaatac aacttaacaa
    48121 tatccttact tatttattct aagttctctt agacaagcaa tagtaatttc atcttgtgga
    48181 tttaattcat aagctctttc tatatagtaa gttgcatcat caatatcacc attatttaaa
    48241 taagccattc ctaaattgca aagtatttct gggtcttcct ttatcttagc cgcttgttca
    48301 aaatactcta ttgctttatc taaattattc ttataagcct cacaaagccc caattccact
    48361 atagtatcca cttgattagg ctttattatc aatatcttct caagatacat cttagcctgc
    48421 tctatttcgc ccataccctt atatccaagt gctatcataa acagcaagtt ccaccaatcc
    48481 atatgttctt cttctaatgg aagtaacttc tctaatcctt ccttaaactt accttggaat
    48541 acaagtgtat atccttcttc atattgaact ttaaagtcca tcttgcccag attctcttgt
    48601 acctcagcta ctaaatcagc atctaaccct agccttaacg cttcttccca agtcagctta
    48661 gtctttaaat attgaccttg attataataa tggtagccta agtgataata acctaatgca
    48721 aaattctcat caacatcaat taccttttca agcttagcca tggcttcaag taagaatgca
    48781 ttcattgcct tatcatccat atctttttga tactgtttag ctatctcttg gcaaacaatc
    48841 gcatagttat acattgcatc taaatcatct ggatacattg ttataaatgc ttttatatat
    48901 attaaagaat ctgtatgttc accaatctca aagtattttc tcgacatata tcccatatac
    48961 gatttcaaat ccaaatcaat atcttttgat aaagcattta aaaacttctc atactctgca
    49021 ttgtttttaa acttactatc tattccaatt atatatatca ttgcatctgc tatagacatt
    49081 gaatttaact tatcttgagc agtcttttct tttatccctt ttactaatac ctcatttttt
    49141 ataggtacat ctaatccacc ttttggcact tcatagcttt ttaatttaaa ctctccacca
    49201 tgttttatag ttataaaagc taattcttct gcttttttta gcaaatattt ttctatctta
    49261 aaatccattt tatcctccta aatacttaaa tctctacttt atgagtttta gatttctcaa
    49321 atattttttt atcatcatac tcaaataaga ctacattttc tacttctctt atttctgtat
    49381 ttatatcatt ttttaaatcc acaacattaa atctaaatgg ctcaaaatga acatatttta
    49441 ttatatgttt agctggttta tcaacaaaac taggtaaata tgttgataag ttatcattat
    49501 cttgtaaaaa tacatggcat ctattcttaa aatcatccac atctagctta ttaaaaaact
    49561 gtggtatatt tgatgtttta cccagtgata tatagtcata ttttaatata tcgttgaata
    49621 catcattatc tatgttgatt ttttcattat agaaatccat caatatttta tataactgat
    49681 tctttccttg tgccaaatca aagtagccat tttcatcaaa atacttggca aaaacctcaa
    49741 agaatttaaa agggcttttt ttataaaatc tgcctataat atatctcata gataacacaa
    49801 agtttttaga attgtagtat ctctccagta tatcttctat atcctttagc ttcaaagttt
    49861 cttcatagct tatataatta ttgtataaaa actcatatgg agcataatcc ttatatttaa
    49921 aaccatgctc atctgcaact tttctcatac ctgtcccttt aatcatcttt aaaaagccta
    49981 attgcaaatg ctctatacct agattaaata catcattaaa agaattttca aagctcttat
    50041 aatcttcata aggcagtcct gcaattaagt ctaagtgctg atgtatattt ctataagatg
    50101 ctacagtttg tacaacatga gacaactttt taaaatcatc ccttctacct actgcatcaa
    50161 gtgttttttc attcgttgac tgaacaccta tttcaaattg aaaaagacct tctttacaat
    50221 cagccaaaaa ctcaagcatc ttatcatcta tcaaatacgc tgtaacttca aaatggtaag
    50281 ttgtatatcc attatcattt ttcataagga aatccatgat ttccatagcc acttttctat
    50341 ttgcattgaa tgtcctatct ataaacttta tctgtgatac tcttgcatct attaaagctt
    50401 ttaaatcact ctttatcctg tctaaactaa aataccttag acctggaata gtagatgata
    50461 aacagtattg gcagttaaaa gggcaacctc ttgatgattc ataatacact attctatttt
    50521 catattcttt tggattcaag ttttcataag gactaggaat ctcatccaaa tcttgaagta
    50581 attccatagg cttattcact attatctcat tgccttgtct atatacaagt ccatttacat
    50641 catttatttc catctttcct tgcaagtgaa gtactaagtc tctaaaaatt aattctccct
    50701 ctccataaag tatataatcc acaaaatcat actttttcat tgcctcataa ctatcataac
    50761 tcacttccgg tccgcccaaa gctattttag tattaggtct aatttttttt agattatcac
    50821 aaagtttaac tatatctccc acattccaga tatatgttga gaataatatt atatcatatt
    50881 catttttata tatatccttt aaaatatagt ccaaatcgtt atttatcgtg tattctttca
    50941 tattaacttc tatcaaatct cttacaaatt cttttagata tcttatagcc aaatttgtat
    51001 gaataaactt ggaatttaat gttgttaata aaattttcaa atatttctca cctcttttta
    51061 agattatact gtctttttgt accccttata ggttaaaata gtatatgtaa agtctaaata
    51121 atatctatat aattaattta ttaatttcaa gtaatgtgag gaaaggaagt tttttatgga
    51181 attaattgaa aaagtatcta tcaactcttt atcaaaaaga gatttactac ttattataaa
    51241 gtctcttgaa tacactaatg aaaatactca aattagtgat ttcattgacc ttaggaataa
    51301 tatagtaaag gaattatgtt ttttaactga cactaaggaa aaagattttt tagattatct
    51361 agaaaaaaat agcacactct aataacctaa acctttttaa actttaatat ataaatacct
    51421 gatttagaat ttatttttat gcttaatata ttatcattaa aggttttcga cataactgtt
    51481 atttgattaa atatagggtc tattatagta ttaccttctt caagtcttat aagacttctt
    51541 gggtctatgc ttttattata tgccttaact ccaattttat attttctcaa tttaacaaca
    51601 tattcggaca tagaaaaaat aaaatctata ttatcttcat ttaatttact atcttcaccc
    51661 aaaatggata atatagattt ttttatgcta ttaattcctt cattttctac agttctatct
    51721 tccatatata catcatatag aacatttaaa acatttaata aaatactttc ttcatcataa
    51781 tcttcagatt tcaaatcaat gtacttatca attaattgta ttgctttaat ccttgccatc
    51841 tgaaacttta caacatctga tttgtctaat tctatagaaa atcctattat cttgtcttta
    51901 atactctgta gtaattcgac atactctatt ttactattat ttataagatt gtgcataatg
    51961 gaattataca ccacagaacc taataaataa tcaaataaaa gttcttcttt tttaaaataa
    52021 ataacatact tttttattag tttaataacc tcatcttcac ttttttgaat attagtattt
    52081 gataaaatta gtggtaataa aatatttaaa aaattgatat ttcctatttt ttctccataa
    52141 aatctatagt aaataagttc atctctcagt ctattttcgt ctatatacac acatctttgc
    52201 gttttatcat ctattaagga ttttgtattt attatagtta aatctttaag agccctactt
    52261 gcactactaa aagaagatgt gtagactttt tttagtaatt cgtcattcat aaatatatcc
    52321 tttctgaaaa attgaatgta aataaattgt tattaaaaaa aaagagacag cccgtttaag
    52381 aagctgtcat atataggggg atagtagtgt acaaaaatca ttgtaatcta ctatctatat
    52441 attattatac gatactactt ttttatatgt aaagcatcag ttttcgacat ccctatattt
    52501 tacagttata caaattgcgt actaagttca attttattct ttttatttca acattttatc
    52561 atgctacatt ctttcaggag cgtgcattcc caacaatttc agagaatttt ctaaagtttg
    52621 ttttgttgct aaaactaatg caagtcttgc ttttcttatt tccaaatttt ctactaaaat
    52681 tggattgtca tgataaaatt tattaaacgc ttgagctaaa tctagcataa atctagtcac
    52741 tatatgtggc tcatttttct ttagagcaag taaaatattc ttattgaatg attctataac
    52801 tctaagaact tctgcactat cttcatcagc taatagacta taatctatat cagaagtcac
    52861 ttctacttct gcttttctta aaactgcaca gcatctagca tgtgtatact gaacatatgg
    52921 tccagtctca ccatcaaaac taagcgttct ttcccaagag aaggtatagt ctttaattct
    52981 actattagat aactcttgga atactactgc cccaacacct acttgcttag atatttcatc
    53041 cacattttta gcatttgggt ttttagctag tataatttcc ttagttttat ctatagcttg
    53101 atttaaagca tcttctaaaa atactactct tccttttcta gtcgacatag taccttcttc
    53161 aagtgcaacc ataccaaatc caacatgaat taaatctttt gcccaatcat atcccataag
    53221 ctcaattacc ttaaaccatt gttggaaatg taagttttgt tgagaaccaa ctacatatat
    53281 acacttgtca aaatcataag tttcttttct atatattgca gcagctaaat ctcttgtcat
    53341 atataacgtt gagccatcat tcttagtaat aagtgctggt ggcattttat actcttctaa
    53401 atcaacaatt cttgctcctt tagactcttt taaaagattt ttctcttcta atagttcgat
    53461 aactctgttc attttatcag aataaaaact ctctcctgct aaagaatcaa attctatatc
    53521 taaaagttta tacactctgt taaactcttt taaactctca tttctaaacc attgccataa
    53581 ttcttgtgct tcttcgtcac cattttctaa tttattaaac catgctcttg cttcatcttc
    53641 catttctggg tgttgttcag cttcatcatg gaatcttacg tataatttta gtaattctgg
    53701 aattggatta gactctacta cttctttttc tccccacttt ttaaaggcca ctattaactt
    53761 accaaactga gttccatagt cacctaaatg atttattctt atagtcttgt atccttgcga
    53821 atcatatatc ttatataaag cattacctat aactgtagtt cttatgtgcc ctatatggaa
    53881 aggttttgct atatttggag aagaatattc aactattaca gttttatctt ttccaaaatc
    53941 actatgtccg taattttctt tttcatctaa aactttttta attacagttt ctgctaattg
    54001 agatttattt acaaagaaat ttacatatcc gcctaattgt attacttttg ttatctctcc
    54061 acttggttct atactttccg ccaattcact tgctatcatg tttggagctt ttctaaacac
    54121 tttggctaat ttaaagcatg gaaaagcata gtcccccata tccttatttg gtggaacttc
    54181 tataagtgcc tctatctctt ctttcgataa atcttctatt ttttctttaa ggcaatttga
    54241 tattgccacc ttaaaatctt gcattctgtt gctcctctct acattgttcc tggtttatat
    54301 tgtgcttatc tctttatgtt aaatcaaatt tcgtgttttt tcaagtattt cattacagtg
    54361 taaaacactt tagtaattat gttttctgta tatattaaaa atactataat tgcagttttt
    54421 aatcacacta ttattgattt tttataagac ttttttctat tttattgttt cttgtactat
    54481 ccagttgact ttatatactt tttttaatct tttatctatt ttcatacgtc aatgctaatt
    54541 ttatatactt ttcttttaat cttttattaa tttttataca tcaatgttaa ctttatataa
    54601 gtttttttaa tcttttatct gttttcatac atcaatgtca gctttatata ctttttttac
    54661 ttttttatct atttttatac atcaatatta gctttatata ctttttttaa tcttttatct
    54721 attttctata aatctttttc actcttaact tttattcctt cttcttctaa tagccttgct
    54781 gttatacctt tccctggtat ctttttacct gaaaaactac catcatatat tagattacat
    54841 ccacaagagg ggctaccctc ttttagtatt gcttcgctac atacatataa atttgctatt
    54901 tttagagttt cattagcccc tcttacaaag ttttctgtta catccttatt atctttagta
    54961 attacttttc catcaactat ctcagcaggt ggtcttggtg tagatagacc acctaactgc
    55021 tctgggcata caagtgtaaa ttctttgcct tttaaatatt ccttgacttt ttcattatca
    55081 ttattatcgc cactatattt acaatttatt cctattaaac atgcagatac caatatcata
    55141 aaaatgtgct cctatacaaa ttcttaatat attctcatac aaattactaa tgtattctta
    55201 tacaaattat tttaatttaa caattgttac tcctgctcca ccttctccat attgaccacc
    55261 tctttgtgat tttacatgtc tatttcttct taaaatgtct tgtagccctg cttttaatac
    55321 tcctgtacct attccatgta ttactgttac agactctaaa cctgccacat atgcatcatc
    55381 aagatacttt tctacttcca ttatagcttc ttctaggttt aaccctctaa ggtcaacttc
    55441 attttttaca ctaccagatt ttgaccttat aatgtttctc gttgatttag ttacatttgt
    55501 tgaaacatct ttgcgtgttt tttgtaaaga cttaaaaggt agagtcatct tcattatacc
    55561 aatttgcact actgcttctt ttctcttttt atctactgaa actacactac cattttgatt
    55621 taaagttatt accttaactt cttcacctgg ctttaagtct tttatctcct tatttgatac
    55681 ctttggaact atcatacttt ttactgttgg ctgaagactt cccatagagc tggtcaattc
    55741 ttttctaagt tcttctatct tacgattctt ttcttttgaa gctctttctt gttcaagact
    55801 tctaagttct tttattataa tatctacttc ttctttagct tgtcttataa ttgaaaatgc
    55861 ttctgattta gctttttcaa tcatcttatc tctttgactg accaattttt caagcttttc
    55921 gtcatactct actttcagtt tttcaatctc ttcttttaat ctttcagctt cttctctatc
    55981 ttctactgct ttgattctat tcttttcaac attctgtaac acatcttcta aagctatgtt
    56041 ttctgtattt atatattctt tagcacgtga aatcacataa tcacttagcc caagttttct
    56101 agaaatctca aacgcatttg attttccggg aacacctata agtaacttat atgttgggct
    56161 taaagtttct atatcaaatt caactgctgc attttctaca cctggtttag taagagcata
    56221 atttttaagc tcactatagt gagtggtagc tatacattta gctcctacag aatttatatc
    56281 ttctaatact gctattgcaa gggcagctcc ttcaacaggg tctgttcccg ctcctaactc
    56341 gtcaaatata actaaagagt ctgctgttac gttttgtaat atagatacta tattcgtcat
    56401 atgagatgaa aatgttgata gactctgttc aatactctgc tcatctccta tatctgcaaa
    56461 tacattatca tatacacaca tactacttcc ataatcagca ggtatatgaa gtccactttg
    56521 tgtcataagt gcaaatagac ctacagtttt tattgtaaca gtcttaccac ctgtgtttgg
    56581 acctgttatc accaatgtat ggaagtctct tcctaaataa attgtatttg ctactacctt
    56641 ctttttatcc agcagtggat gtcttccatt ttttatgttt aaatatttat cctcatttaa
    56701 agttggttct attcctctca tctgtataga aagttttccc tttgaaaatg caaagtccaa
    56761 tcttccaagt atctcttgat tagaaattaa gtcttcacta acttctccaa ccatagcaga
    56821 aagctctgat aaaattcttt ctatttcttc ttgctcacct agttttaact gtctcaattc
    56881 attgttcatc tctactatag tcattggctc tataaaaagt gtcgcaccag aagatgactg
    56941 gtcatgcact ataccagcta cttgcgacct atattcagat tttacaggta ctacaaatct
    57001 atcacctctt agagaaatta ttgcatcttg taagtacttt tgataagtag ttgaacttat
    57061 tattgaattt aattttgacc taatggattg atttttttgg gctattcttc ttctgatatc
    57121 tctaagtgta cttgaagcat tgtcagatat ttctacttca ctaattatag cattgtatat
    57181 ctggtcttct atgtccttgt atacatatag agaatttgat agagattgta ttattggata
    57241 attaaaatct tcttcatcac tactagataa gctatttctt aatcttctag ccactcttaa
    57301 ggtatctgct atcataatca gacttcctgg gtcaagagaa gcacctatat aagctctctt
    57361 taccttatct tctatatcat gaataccttc caaccctact gaacctctct ttataataat
    57421 tgattgagct tcactagttt cttctagcat agatttcact tctacaaaat cagtatttgg
    57481 tactaaattt tctatatatt ttagacctaa agaagatgag gctttcttct ttaacaaatc
    57541 tattatttta ttgtattcta gaactcttaa cgatttctca ttcattttct atattcacca
    57601 tcctcgatat tttaactttt ttatttaatt ttaaaaatgt atgttttcaa gcatttccaa
    57661 taaaaactgc aagttttatt tctttttcat ttctaaaatt attatatttt ttctctttta
    57721 gatatatttt acacatattt tgtattatac tatgtatgta gctatctata tattatacat
    57781 aaacatgctt tatttgtaat ataatctgtt aaaaaatctt ctttaatatt tttaaaggag
    57841 tttattttag cttataaaaa aaaatagcta cttatataga taaattaagg aggaacatga
    57901 gtcatgggaa acgtattaga aggattaaaa ccagaaagtg tttttaaaaa ctttgagaaa
    57961 atatctcaaa ttcctagagg ttctggaaat gaaaaaggca taagtgattt tttacttagc
    58021 tttggtaaaa atttaggtct tgaaactatt caggatgaaa gcttaaacat aataataaga
    58081 aagcctgcta caaaaggata tgaaaattgt ccaggtgtag ttcttcaagg acatatggat
    58141 atggtttgcg aaaaagaaaa gaatgttgaa catgattttc ttaaagaccc tataaaatta
    58201 agaattgatg gtgacatgat atatgctact ggaactactc taggagctga caatggtata
    58261 gctgttgcta tgggtatggc tatattagaa gataatactt tagagcatcc tgcactagaa
    58321 gttttagtta ctgttaatga agaagatggt atgaatggtg cagatgcact agaccctagc
    58381 ttaattaaag gtcaatatat cctaaatatg gactccgagg aggaaggata cttattagtt
    58441 agctgtgctg gtggtaaaac ttgtgttgtt agtttacctg ttgaatacaa ggaagttaaa
    58501 ggtgacaaac aaggattatt agttgaggtt actggactat taggtggaca ctctggtatg
    58561 gaaattgttt tacaaagagc aaatgctaac aaagcaatag ctagagtatt aagtgtattg
    58621 aatgttgatt atgaattagc ttctgttgat ggtggtacta agcacaatgc tatacctcgt
    58681 gaagctaaat gtgtaatagc tgttaataaa gctgatgttg aatctgctaa aaaacaaata
    58741 aatgacattt taactgcatt taaacatgag tttactactt ctgaccctgg catgacttac
    58801 tcagtagctg aaacttctgt agacaaagtt cttactaaag attgtaaaga aaaagttgtt
    58861 caaatgtctt gcttaactcc tcatggagtt caatcagtaa gtcttgatat agaaggatta
    58921 gttgaaagtt ctactaactt cgctataatc gaaactaaag aatctactat tgaattttta
    58981 acttctgtta gaagttctgt tatgtctatt agagatgaaa tcgctgatag aataagactt
    59041 ttggctcaag ctttaggtgc taattacgac cttatagctc aatacccagc ttgggaattt
    59101 aaaaaaggct caaaattaga aaaaatatgc agtgaaactt atgagaaatt aactggtaaa
    59161 gttcctactg ttatggctct acatgctggt cttgagtgtg gattattact tgataaactt
    59221 cctcatgctg aagctatctc tataggacca gatatgtttg atgttcatac tccaaatgaa
    59281 catgttagta tcccttctgt agctaatgtt tgggattatg ttattgaaat attaaaatct
    59341 atgaaccaat attaaattta agttttgatt ctaagtagat tttaatttta agtaatctat
    59401 ataaaattaa ctctgtaaat taatactact gttagtataa tatttacttt caatattaac
    59461 ttatagaaat aaagcctaat gatagataat aacatgtatc attaggcttt attgtaattt
    59521 aaatattatt aagctttatt ctgttttaaa attaattctt atactatttt aaatctattt
    59581 cttccttgtc tcttagaatc ataaagagca ttatctgcta tttgatacag ttcatcatac
    59641 tcttgaccag gctttgctat aactaccccc atactaatag ataaactttc tccatttggc
    59701 aacttaatga gatgaatatg attgtataca tcattacatt tattaataag ttcttcctta
    59761 tttattatat cattgatata aacactaaat tcatctccac ccaaacgacc tactatatca
    59821 ttttttgcaa aactttcatc aagtaccttt gcaacatcaa taagtacttt atctcctaac
    59881 aagtgaccat aagtatcatt aatcgactta aaataatcaa tatctataat caataaagca
    59941 ccattttttt cagttgataa attcaacaaa ttttgtgcag ttaattttct aatatatgct
    60001 gcattataaa tattggttaa actatccctt tgagccttat ctaacaatct attcttttcc
    60061 tctttttcag aatcaatatt acatattttc ccgattacat acattggtat ttttctatca
    60121 ttataaatta tttttgaagt aactctaatc caatgaaaag tatcatcaag cataatagaa
    60181 tacatttcag ttgttccatt ttcttgtgat tttattacat caaaaaactt tttattatat
    60241 tctttcctta ttttatctct ttcattttct atattgccat tttcactttc cttatcattt
    60301 ttatccaaac cataataagt ctcaataccc ttttctggca tagaaattat caatttatct
    60361 tgcttataat catactcaaa aaagtattca cttgtcattt catacaactg agtatgtttt
    60421 ttgagttcta aagatatctt ctcgttcata cttgtacgag tatgcaaacc aaatgccata
    60481 aatccaataa tacaaattaa taaactggct acaataaaaa tagtctcaat tggattctgt
    60541 tgcataatat aaagcaacga gaagtcttgt ctatatgtag tattttggta gacaattgat
    60601 tgcatttttt ctgatgaaat acttataata gctttattaa atatattcaa taattcttgt
    60661 ttatcttttt taattatacc aaaacaaata ctctttgctt tataagtttg aggaactaga
    60721 cgcacatttt tatacttagg ttgattcata tagtactgca tagaataacc ttcgccataa
    60781 gtgtaatctg catagccttt attaacagct tctatacact cttctaaatt atcataccaa
    60841 actatattct cttcaaactg accataataa ttaatacctt tggctagagc tacacgtttt
    60901 ccatctaaac tattctcatt taatttatta ttaatcatca tagtatattg agtagaaaga
    60961 aaaggccgag acataactat attatgttca cgagagtatt cataatcata aatcattcct
    61021 gctacaatat caatttcacg ctttttagtc atgttttcta aatcttcttg agattttaca
    61081 ggcacaagtt caaattctaa gcctgtttta tcacatatat gatttaacaa atcaattgat
    61141 atacctctta attcacctgt atctttatct atattttgaa atggtggttt gtttaaaagt
    61201 actcctactt ttaatttttc tgtctcttta atgtaatcag tttcttcatc agataaaaac
    61261 atttgattgt tctctggtgt aaagtatttt tcatataaag tagttgaaaa atatgggtct
    61321 ttctgttcaa tattattaat tgctgagtta agcttttgag ttaaatttgt actaccttta
    61381 ggagtaatga aataaaatgg ttttgatgaa aattttgcta cagtacgtac accttctagt
    61441 gggttcatac taacattaag aagtacatct gctttaccac tctttagtgc ttctagttgc
    61501 tcatcatttg ttttacaata taccaattca ggtgacataa gattcatttt acaatactca
    61561 tctaactctt taagtctagt atcagaattt tctattacag caatacggat ttttttcaca
    61621 tcttgtgaac tcattatgtt atcaatatct gtattttcat ataatgcttg tagtactgta
    61681 tttgctactc cataactatt tcctgcataa ttatatattt tttgcattgg ctcattgtat
    61741 aacatacctc ccattatatc aatttcacca ttttctaaca tcttcattaa ttttgtaata
    61801 gattcattta tctctccagg aacttgaaca aattcataat tccatccagt atactgtgca
    61861 atttctagta aatattcata tgtataccca aaatagttac cttgctcatc tatttcagtt
    61921 aaaccttttt gaataggata agcaacacga atggtacgat tatcattacc tatagcgttg
    61981 ataccaaaag aaaatatgca agaagtgaat atagatgtaa ttacaataat taataaaagt
    62041 aattttttta aattcactac acaatcctcc atatagttaa gatttacagt ataaattact
    62101 gcataaactt gtagacatgt aaaaatagat tagtatataa aaggatatat aaatctactt
    62161 agaaatttaa atatccttaa tatatacaat atatttaaat tataactcat ttatatgtct
    62221 attacaacaa atttaccctg ctaaatacct actctatatc ccataatttt acaagttgta
    62281 gtgaagttta agttctatct cacctcaaat ttagcagtgt ttctatttac agttctttta
    62341 tatttcacat ctggataacc caatataaat gaagtgataa cagcttcgtt ctcgctcatt
    62401 cctaacagtt cttttatctt tggctctaca tttgatgcca taacaaaaaa tccattataa
    62461 caaattccta atccttgaga atatgccata agttccatat tagatgcagc cagtcctcca
    62521 tctacataag cacttccacc tagagacata tctcctacaa ctaccataag tagtggggca
    62581 tcataaaata aagaatcatt tccgttctct ttatatcttt tgtacatttt cttaaatgta
    62641 tttttataga tagaacgtac agggtcattg tcatcagtat ctaatgccaa attataaaga
    62701 ccttgtattg ccagttcttt cacttctttt agcttttctt ttactaatat gtatcttata
    62761 ggctgtctat taccacctgt tggtgtgtat ctaccagctt ccactatgtt ttttattttt
    62821 tcttcttcta cctctatatc tttatattgt cttatacttc ttctaaattt aataaaattc
    62881 aataactttt cgggttctaa ttcaaaactt tcacgattgt attctattac ttcattttta
    62941 tcaaatcctt gcatttctat tgcatctgtt gggcaaattg ccatacagtg accacagtta
    63001 atacagagaa tatttttaac ctttgcttta tcatccttaa cttttattgc atttacaaga
    63061 cagtctgatt cacacatccc acagcctaca catttttcta aatttatgtc tatcattcca
    63121 aattattcct ccttttaaaa aatttacttt agtataaaac aaatagttgt ctaagtcaat
    63181 ttttattttt ttaacaatat actagcctta actacacttt aatggagaaa aatatacata
    63241 aattggaact taaattagaa cttattcaaa gtaaaagata aaagaagtag atgtctaaaa
    63301 gtgaactttt tagttaatta gatattcttt attgtatgaa atcaagtgga tatttatcat
    63361 ttcaacaata aaaaaggctt acctctattt tggatatgtc cctgtcaagt agacagacta
    63421 aaaaaaggct atctttatgc actgagatag ccccttttcc caactactat tgtttatctt
    63481 aatattagtc aataaattta atttgctata acctgtaagt tacattttaa tttgatgtaa
    63541 ctttaaatta catttttaag taatatcttt aaattatatt cttaaaatat atcattaaac
    63601 tacattttaa ttaatctaaa aactatatct taattaatct aagaaactta cattataatc
    63661 ttttagtttc ccattatcat atacaatctt tgcactaaag atatttccag tcttagccca
    63721 atttaagaac agcctgtcac cttcccacat atttaaatcg aatattttat cattatcaac
    63781 ccaaattaaa tttccttctt tacaatcatt taaatctttt tcagataatt caccttcaaa
    63841 ctcatctgag aaatacaaaa acatatacca atcttcttca tctttaaatt ttggaaaagt
    63901 tatcaaccct ttatatgaca atgatttaag agtaagacca gtctcttctt ttacttctct
    63961 aattacacat tcttctggag tctctccttg ctcaaacttt cctccaactc ctacatactt
    64021 accttcatgt atatcatctt tctttttatt tctgtacaac atcaaagtct tgtcatcttt
    64081 ttctatataa caaatagttg taagtttcat gtatctcgcc ttgctttctc tttatattct
    64141 caactaatta ttttcagcac tttcaagcac atcacttata ttcttcattg cattaagaga
    64201 aagctctgca aattcatcaa atggaatccc tatatcttct atacttctca ttgcatctct
    64261 atttacattt gaagcaaatc tcgtttcttt cattctttta ataacagact tagtctttac
    64321 actggtaagt tttttatctg gataaactaa agctatagca gttataaacc cagttatagg
    64381 gtctgctgca tatattgctt tttccattag gctagttctt gtaactccca ttccatcatg
    64441 atgaccaagt attgctctat acatttcttc gtcaccgaag ttttcttttt taagcagttc
    64501 tactgttgtt gtagcatgtc cctcacatat ttctcctggc tttcttccaa ctatatctga
    64561 atccaaatca tgtaaaagac ctgccactgc ccactcttct tctttttctg gctctagtct
    64621 tttggctaat tctctcataa cagcttcaac agcataacaa tgttttctca ttctatcagt
    64681 ttctaaatac ttaaataaaa tttctaaagc ttcttctctc attttcacca atccccctgt
    64741 tttttcacat atatgatatt tttatatatt aatacacata aattcaagtt tttatatata
    64801 ttcatgtata tttttcatgt tatatctatt ctacaccttt atagtagtgt cctgcaactg
    64861 ttccaatttc atctaatttc ttatacaagt ctgtagccct ttttccaagt ttataattat
    64921 tactcaatac ctcaatgtga gcttcaataa cctttttaac catctcacta tcttcaactg
    64981 taaaatctat cctaaataca tctattccaa tttcctctaa ctcatataga ttgtccaaca
    65041 tacaagtaac cttcgaattg taaattgtac ttctacagaa tatatcttgt gttatcctat
    65101 aacttgcatc cgtaaagtct cttaaagcat aactactttc cttacattta gcaactctct
    65161 tgtccttttt acaatcccta acaactactc ccataggaca gtattctgta accatcatag
    65221 gagcatatcc atatattact gcttcaattt ctacatctgt atattttaaa gtttgtttaa
    65281 tttcatttaa gtttagttct tgtgacaaac aaactgtact tgctccctct tctttgaagt
    65341 ggtttatact ttcactattg aatgcattta agtagcaatc tatatttaag tcttttttct
    65401 taaagtattc catagagccc cagttaccaa tttgaacctt attaattcct tttatattat
    65461 ccaatatctc aaattgcttg tactctttat ttctaacaat ccttggtgct gaatagatta
    65521 attttttgtc agtgactgct gtattagagt ggttcttatt caaagtatct attttacttg
    65581 tatttgacaa agtatatatg ttatttgtat ttgaacccat tagttcttta ggaataccta
    65641 aagatgaaga agtatataaa tccatagaaa tacttgaatc tatagaaata cttgagacca
    65701 tttgtaaagc ttttgataat gtatttatat cttcataata gattaaatct acattataat
    65761 ttaatactgc ttctaattgc tccaagttct taactttaac tctaatctta ggaactgtat
    65821 caagtttttt ctgtttattt actctatttg gctggtattt taatctattc ttcttaaatt
    65881 ttctatcttt tatctttatt ctttcttcat ctaaaagctc aatacaatcc cttctcatct
    65941 gatttaacac acttataggc atacttacac cttcatccag gtcaatttct atctctttta
    66001 acttatatgg tgtatttcca agcttttgaa tttgtgtttc tattttttct ttacttaaag
    66061 ccacctttat agctttttca actattttgt ctccttctac caccgattta tttccacaaa
    66121 aatcacttat aaaaagctct ggctttttac ctagtttaat actcactttt gcacgtatat
    66181 tagtttttat gaactcttta tcttgctgaa atgtagcttg tactctatcc atcaattcgc
    66241 tgtcagaagt cttatatata agctgatttc tctttgcttc tccaacaaaa tctaattcta
    66301 tcaaatcccc tttatgtgct attgtagtta tctctccatc ctttattatt cttccaatag
    66361 tacctccacc taagttaatt ccatcacctt tcttaagcgt attttctagc attactctaa
    66421 gtcttttgtt ctttctatta taatctaaaa cttttccaat atatagccct tgattattag
    66481 gtctattaga gttcattata tccttaccaa cttcacctaa tatatatcct tttgtaaact
    66541 ttctattaaa tatggtatac aaattattta ttatttcctc agaaatactt atttttttct
    66601 tatctacaaa ttcattaatc gcttctctat atcctgaaac cacagtagct acatactctg
    66661 gctttttcat tctaccttca atttttaaag ataatacacc tgcttcaatt attttatcca
    66721 aatcttctat agtacttaaa tctctaggac tcaacaaata atctccatta ctttttacta
    66781 cttctcctgt atttatatct atcaactcat acttttgtct acatggctgt gcacacctac
    66841 ctctatttcc cgacctattg cctatcatac tactcataag gcattgaccc gagtaacata
    66901 cacataaagc tccatgtaca aaaacttcta tatctacagt tgtattttca caaatccatt
    66961 ttatctcatc aacatttaat tctcttgcta aaacaactct atcaaaccct acactctgaa
    67021 ggtattttac atcttctaag gaatgtgcta ccatttgagt gcttgcatgt agttcaaagt
    67081 caggtaatag tttttttata agtcttgcca ttcctatatc ctgtactatt aaagcatcaa
    67141 tatctatatc gtataaaaat tttacatact ctacaaaatc ttctatttca tactgtttta
    67201 tcaatgtatt tacagtaaca aagacttgca ctccccttat atgagagtat ttaacagcct
    67261 caatcagctc atttctatca aagttatttg cagatgctct agcactaaag tctttaccac
    67321 ctaaatacac agcatttgct ccgttttgaa ctgctgcttt taatgaatca aaagagccta
    67381 ctggtgcaag taattctatt tttttcatat ttttctcctc tatttatact tgttaggtta
    67441 atataa
//
LOCUS       DAMJZE010000002        61829 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJZE010000002 DAMJZE010000000
VERSION     DAMJZE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.002"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            223..339
                     /locus_tag="PC541_000055"
     CDS             223..339
                     /locus_tag="PC541_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDF2837545.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            503..1570
                     /locus_tag="PC541_000056"
     CDS             503..1570
                     /locus_tag="PC541_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2837546.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1762..2292
                     /gene="rnmV"
                     /locus_tag="PC541_000057"
     CDS             1762..2292
                     /gene="rnmV"
                     /locus_tag="PC541_000057"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2837547.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2463..3332
                     /gene="rsmA"
                     /locus_tag="PC541_000058"
     CDS             2463..3332
                     /gene="rsmA"
                     /locus_tag="PC541_000058"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2837548.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3398..4660
                     /locus_tag="PC541_000059"
     CDS             3398..4660
                     /locus_tag="PC541_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837549.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4811..5926
                     /locus_tag="PC541_000060"
     CDS             4811..5926
                     /locus_tag="PC541_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2837550.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6112..7008
                     /locus_tag="PC541_000061"
     CDS             6112..7008
                     /locus_tag="PC541_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2837551.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7078..7875
                     /locus_tag="PC541_000062"
     CDS             7078..7875
                     /locus_tag="PC541_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837552.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7930..9282)
                     /locus_tag="PC541_000063"
     CDS             complement(7930..9282)
                     /locus_tag="PC541_000063"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2837553.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9501..10379
                     /gene="purR"
                     /locus_tag="PC541_000064"
     CDS             9501..10379
                     /gene="purR"
                     /locus_tag="PC541_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2837554.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10535..10813
                     /gene="spoVG"
                     /locus_tag="PC541_000065"
     CDS             10535..10813
                     /gene="spoVG"
                     /locus_tag="PC541_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2837555.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11026..12405
                     /gene="glmU"
                     /locus_tag="PC541_000066"
     CDS             11026..12405
                     /gene="glmU"
                     /locus_tag="PC541_000066"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2837556.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12500..13450
                     /locus_tag="PC541_000067"
     CDS             12500..13450
                     /locus_tag="PC541_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2837557.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14288..14373
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14535..15056
                     /locus_tag="PC541_000068"
     CDS             14535..15056
                     /locus_tag="PC541_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2837558.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            15207..16883
                     /locus_tag="PC541_000069"
     CDS             15207..16883
                     /locus_tag="PC541_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2837559.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16895..18103
                     /locus_tag="PC541_000070"
     CDS             16895..18103
                     /locus_tag="PC541_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2837560.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18118..19830
                     /locus_tag="PC541_000071"
     CDS             18118..19830
                     /locus_tag="PC541_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2837561.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19823..20731
                     /gene="pilO"
                     /locus_tag="PC541_000072"
     CDS             19823..20731
                     /gene="pilO"
                     /locus_tag="PC541_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2837562.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20758..21324
                     /locus_tag="PC541_000073"
     CDS             20758..21324
                     /locus_tag="PC541_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2837563.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21361..21888
                     /locus_tag="PC541_000074"
     CDS             21361..21888
                     /locus_tag="PC541_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2837564.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21909..23447
                     /locus_tag="PC541_000075"
     CDS             21909..23447
                     /locus_tag="PC541_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837565.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23547..24629
                     /locus_tag="PC541_000076"
     CDS             23547..24629
                     /locus_tag="PC541_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2837566.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24610..25392
                     /locus_tag="PC541_000077"
     CDS             24610..25392
                     /locus_tag="PC541_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2837567.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25494..26144
                     /locus_tag="PC541_000078"
     CDS             25494..26144
                     /locus_tag="PC541_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2837568.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26158..26718
                     /locus_tag="PC541_000079"
     CDS             26158..26718
                     /locus_tag="PC541_000079"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2837569.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26735..30121
                     /gene="mfd"
                     /locus_tag="PC541_000080"
     CDS             26735..30121
                     /gene="mfd"
                     /locus_tag="PC541_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2837570.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30152..31147
                     /locus_tag="PC541_000081"
     CDS             30152..31147
                     /locus_tag="PC541_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2837571.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31271..31810
                     /gene="spoVT"
                     /locus_tag="PC541_000082"
     CDS             31271..31810
                     /gene="spoVT"
                     /locus_tag="PC541_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2837572.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31930..33543
                     /locus_tag="PC541_000083"
     CDS             31930..33543
                     /locus_tag="PC541_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2837573.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33650..35098
                     /gene="mazG"
                     /locus_tag="PC541_000084"
     CDS             33650..35098
                     /gene="mazG"
                     /locus_tag="PC541_000084"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2837574.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35187..35465
                     /locus_tag="PC541_000085"
     CDS             35187..35465
                     /locus_tag="PC541_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2837575.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35537..35779
                     /locus_tag="PC541_000086"
     CDS             35537..35779
                     /locus_tag="PC541_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2837576.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35841..36095
                     /locus_tag="PC541_000087"
     CDS             35841..36095
                     /locus_tag="PC541_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2837577.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36096..36629
                     /locus_tag="PC541_000088"
     CDS             36096..36629
                     /locus_tag="PC541_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837578.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36635..36919
                     /locus_tag="PC541_000089"
     CDS             36635..36919
                     /locus_tag="PC541_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2837579.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37032..37952
                     /locus_tag="PC541_000090"
     CDS             37032..37952
                     /locus_tag="PC541_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2837580.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38042..40414
                     /gene="spoIIE"
                     /locus_tag="PC541_000091"
     CDS             38042..40414
                     /gene="spoIIE"
                     /locus_tag="PC541_000091"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2837581.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40765..42657
                     /gene="pepF"
                     /locus_tag="PC541_000092"
     CDS             40765..42657
                     /gene="pepF"
                     /locus_tag="PC541_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2837582.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42771..43736
                     /gene="galU"
                     /locus_tag="PC541_000093"
     CDS             42771..43736
                     /gene="galU"
                     /locus_tag="PC541_000093"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2837583.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44236..45840
                     /gene="rho"
                     /locus_tag="PC541_000094"
     CDS             44236..45840
                     /gene="rho"
                     /locus_tag="PC541_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2837584.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            45993..46193
                     /gene="rpmE"
                     /locus_tag="PC541_000095"
     CDS             45993..46193
                     /gene="rpmE"
                     /locus_tag="PC541_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2837585.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46338..47231
                     /locus_tag="PC541_000096"
     CDS             46338..47231
                     /locus_tag="PC541_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2837586.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47249..48097
                     /gene="prmC"
                     /locus_tag="PC541_000097"
     CDS             47249..48097
                     /gene="prmC"
                     /locus_tag="PC541_000097"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2837587.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48141..49205
                     /gene="prfA"
                     /locus_tag="PC541_000098"
     CDS             48141..49205
                     /gene="prfA"
                     /locus_tag="PC541_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2837588.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49334..50014
                     /locus_tag="PC541_000099"
     CDS             49334..50014
                     /locus_tag="PC541_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2837589.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50043..51083
                     /locus_tag="PC541_000100"
     CDS             50043..51083
                     /locus_tag="PC541_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2837590.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51100..51549
                     /locus_tag="PC541_000101"
     CDS             51100..51549
                     /locus_tag="PC541_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2837591.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51566..52018
                     /gene="rpiB"
                     /locus_tag="PC541_000102"
     CDS             51566..52018
                     /gene="rpiB"
                     /locus_tag="PC541_000102"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2837592.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52054..52683
                     /gene="upp"
                     /locus_tag="PC541_000103"
     CDS             52054..52683
                     /gene="upp"
                     /locus_tag="PC541_000103"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2837593.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            52978..53793
                     /locus_tag="PC541_000104"
     CDS             52978..53793
                     /locus_tag="PC541_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2837594.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54019..54456
                     /locus_tag="PC541_000105"
     CDS             54019..54456
                     /locus_tag="PC541_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2837595.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54641..54868
                     /locus_tag="PC541_000106"
     CDS             54641..54868
                     /locus_tag="PC541_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2837596.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54873..55259
                     /locus_tag="PC541_000107"
     CDS             54873..55259
                     /locus_tag="PC541_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2837597.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55262..55966
                     /gene="atpB"
                     /locus_tag="PC541_000108"
     CDS             55262..55966
                     /gene="atpB"
                     /locus_tag="PC541_000108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2837598.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56035..56295
                     /gene="atpE"
                     /locus_tag="PC541_000109"
     CDS             56035..56295
                     /gene="atpE"
                     /locus_tag="PC541_000109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2837599.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56418..56915
                     /gene="atpF"
                     /locus_tag="PC541_000110"
     CDS             56418..56915
                     /gene="atpF"
                     /locus_tag="PC541_000110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2837600.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56912..57457
                     /locus_tag="PC541_000111"
     CDS             56912..57457
                     /locus_tag="PC541_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2837601.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57474..58976
                     /locus_tag="PC541_000112"
     CDS             57474..58976
                     /locus_tag="PC541_000112"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2837602.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59004..59882
                     /gene="atpG"
                     /locus_tag="PC541_000113"
     CDS             59004..59882
                     /gene="atpG"
                     /locus_tag="PC541_000113"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2837603.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59895..61289
                     /gene="atpD"
                     /locus_tag="PC541_000114"
     CDS             59895..61289
                     /gene="atpD"
                     /locus_tag="PC541_000114"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2837604.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61292..61552
                     /gene="atpC"
                     /locus_tag="PC541_000115"
     CDS             61292..61552
                     /gene="atpC"
                     /locus_tag="PC541_000115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2837605.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25000 a         6574 c        11480 g        18775 t
ORIGIN      
        1 taattataaa ctaaagtaaa taaaatattg agcgattatt aaaaagatat atatataatt
       61 taatttgagc agtttgttat atattataaa aaaagagaaa aataaaaggt aaattgtttc
      121 tggataactt aaaattggaa gtaagaatat ttaactagag aagaattatt taagtttagg
      181 ttagctctag gagtagctga ttttatagga acaatagatt ttatggatga tgatataaac
      241 atggtagcaa ttagaccaga agatataagg gtagaattta atagagattt caaagaaatt
      301 atagacctca aactcagacc gtatcgatat aaaaactaaa gttagatgat atagtgttgt
      361 agataggacc atcagctgtt atggcagtat tagtgctttt aacaaatata atagtaaaac
      421 ttttgtatga attgataata aaaaaattaa aaaccaagac ataaaaatgg caacaaaaag
      481 aagttgaata agggaggtaa aaatgaaatt taagaagtta gtctctttgg gaatattatc
      541 aacactttta ttgagtgtaa taactggatg tagtaatgtt tcatcaggtt caaacttaaa
      601 gtctaataat aagacaaaag gagagttaac agtttataca gcaattgagg aagatagtat
      661 agatacatac ttagctacat ttaaagagaa atatccagat attaaattga atgtacttag
      721 attatcagta ggaggtataa cagcaagatt attagcagaa aaagataatc ctaaagcaga
      781 tgtgttatgg ggtgttgcag ctacaagttt gataatttta gatgacaaag aaatgctaga
      841 gccatacgct ccgaaaggct ttgataaggt aaattctgca tttaaagatg ataagaaagt
      901 tccttcttgg gttggaatag atgcttggat gactggtata acagtaaata ctaaagaatt
      961 aaaagctaaa aatatatcag ctcctagttc ttatgaagat ttaataaagc cagaatataa
     1021 agatcttata tctatgccta gtccagcttc ttcaggaaca ggatatttaa cagtatcaga
     1081 actcatacaa attatgggtg aagaaaaagc atggcagtat atggataaat tgcataaaaa
     1141 tattggtgta tacactcagt ctggttcagc tccagctata ttagcagctt caggagaata
     1201 tcccattggt atatcttttg gaaatagagg tataaagtta aaaggtgaag gttatccagt
     1261 agaagtagta tttccaaaag aaggttctgg atgggagata gaatcaaatg cacttgtgaa
     1321 aaaagataat ataaaagaag catcaaaagt atttttagat tgggctataa gtaaagatgc
     1381 aatgaatgag tattctaaga attttgcagt tactacaatt gacacgggaa atccggtgcc
     1441 agaaggattt ccaaagaatc cattagaaca gatgatagat aatgatttaa aatcagcagc
     1501 aagaaataga gaggctatac taaataaatg gatagaaaaa tatgatggaa agacagaaaa
     1561 agagagctaa gccttgcaat aaaatttagt acaaaaagcg cctagaatat aagttttata
     1621 tctaggctct tttgatatat ttatttaaaa tgcatatttc tatataaaaa taaatagttt
     1681 agtaccttga ttaaattaaa taaaaacctt aaaataaaga gatgtaaaga aaaactaaaa
     1741 acgaataaaa ggtgaattta tatgataaaa gaaataatag tagtagaggg tagagatgac
     1801 gttacagcag ttaagagagc aatagatgca gaacttataa ctacaggtgg atttggattt
     1861 ccaaaaggtg taatggagcg tataaaagct gctaatgaaa gacgtggtgt tataatattt
     1921 acagacccag attttgcagg tgaaaaaata agaaaaaaaa tagcatcaga agttccagga
     1981 tgtaaacatg catttctacc gagagaggaa gctaaaaagg atggagatat aggtattgag
     2041 aatgctactc ctcaaagtat aatagcagct ctgaataaag ttagaactga aaatacagaa
     2101 aaaagagatg aatttaagca agttgactta attagaaatg gtcttatagg taatgaagaa
     2161 gcatcactta gaagagatgc acttggaaaa atacttggaa ttggatatgg aaatgcaaaa
     2221 caatttttaa atagattgaa taattatgga gttgaaagag aagaatttat agatgcatta
     2281 aaacaaatct aaacgtaatg tttaatgtat gaaaaatatg tatataaaat aaattgtgga
     2341 attacggtta atttaagatg aattatttat aatatacacc ttattttaaa tcaatgtaaa
     2401 tacatataaa taagtataat taataaaatt aaatatcaaa ataagttaaa aaggagtaaa
     2461 taatggatag actatcttca cataatgcaa caaaggaagt agtacaaaaa tataatttta
     2521 aattttcaaa gtctttaggt caaaatttct tgatagatag taatataatt gataaaatct
     2581 tatctggagc tagaatcaca agaggagata atatcataga agtaggacct ggtataggaa
     2641 ctcttactcg tgagatgggt aaaatagcag aaaaagtagt ggctatagaa atcgatagaa
     2701 atttaattcc tatattgaaa gataccttgt cagatttaga taatacagag gttgtaaatc
     2761 aagatatact aaaagtagac atacaggaat tagtaaaaga taaattaaat ggtgggccag
     2821 ttaagcttgt agcaaatctt ccatattata taactactcc aatagttatg aaatttttag
     2881 aggaagacat acctgtaaca gacatagtag tcatggttca aaaagaagtt gcagatagaa
     2941 tgaatgctat tccgggtact aaagattatg gggcattatc tatagctgtt caatactatt
     3001 gtgatacaga gattgtggca aaagctccaa gacatatgtt tataccacaa ccaaatgtag
     3061 attccacagt tataggtcta catgtaagag ataaaagaaa atatgatgtt cataatgaag
     3121 acatattttt taaaacagta aaagcatcct ttggacaaag aagaaaaact cttcttaatt
     3181 cgttgggagg tcttggtttt cttaataaag atgaaataag agaagtatta aaagaagcta
     3241 atatagacga aaaaagaagg ggtgaaactc ttagtataga agagttttca gttctttcaa
     3301 atataataaa tacaaaagta tcttctaaat agaaggtact ttttttaatt ttgttaacat
     3361 atattgttta gagaaactgt gttaaggggg gattgcagtg ggttctaaaa gaaagtttaa
     3421 aagagactat ataatactag aagctaaaga cataaacttt agatacaaag aacgagtact
     3481 gccaaaagct tttgcaaaag ttgaagtaaa tgatgaaaaa gcaattattg cattatatgt
     3541 agaaaatcta aaatatgtta aagatgggta taaagcagta gcaataaaag atgaccatgg
     3601 attaatagac ttaggaaata taattttaaa tgaacaagga agaggtgaat ttgttttaga
     3661 tttagaagat tatgatgatg agataaaagg tattgcatta ttgtatgaaa gaaatatacc
     3721 tttaattgga tttaaaggaa ataagcttga aaattatgag gacatattat ttcaagaggt
     3781 agaggaagaa gaatatgaag aatatgaaga gttggaagaa gaagacgaag agatagagga
     3841 gaatgaggaa agtgatagag acgaagatag tgaagaagaa tatattatag aagaatatga
     3901 tgaagatgag tatgaagact attgggaaga tgatgaagat attgaagaag gagagttaaa
     3961 agcaagaacg gaagtagaag attctgatga tgaggaaata gaagagttca ataattataa
     4021 ggaaatagaa aattataata gggttaaagt acaggaagaa tatatagaca tagaatatga
     4081 tgaagaagaa gaggaagaag aagtttttga agtaaataga gaagaagata ggtttgtttc
     4141 ctcaaaacct actaaatcaa agaaagttca aaaagatcag aataatattg aaaaaataaa
     4201 aagagctaat ggagtaagtc aaggagagag tggaaataaa caaaccagta cattacttat
     4261 gccaagacaa ataaaaaaag gtttgaaatt atttaaagaa gtaaaacctt ttgtaacaga
     4321 ttatatagat aagacaagat ggtggaaaat agagataaat cctacaacac tgtgtggata
     4381 tacaatgcct tatttaggat atttaaacgt attgaattac actatgtata gtgatgcagt
     4441 tttacattca tataaataca gacactattt atttggagtt cagtatgatg aatataataa
     4501 gagaaaacat tatatttatg ctatcccagg aaatagaaat gaacagccag acaatggaag
     4561 cacaggattt ttaagatatc aagcttgtga taatagaaca aatagtttag gatattggct
     4621 atgttttata gattctagac aaagaaatat agttaggtaa aaataataaa aatgaaaaaa
     4681 tttagactca attcttattt tactgaaaaa attaatgaaa tgtagtacaa aactttaaaa
     4741 actattgata atatttaaat attacactac aatatatctt gaaataccta ttatattatg
     4801 gaggaaaatt atgataaacg agcaaagaat tttaaatgag tttcttgaat tagtacagat
     4861 agatagttta tctttaaaag aggggaatgt tgctaaagta ttagttaaaa aattagaaga
     4921 aataggttgt tcagtagtaa tagacaatgc tggtgaaaaa gcaaatggag aaactggtaa
     4981 tataatagct acattaaaag gaaataaaga aggaaagaaa atattattta gttcacatat
     5041 ggatacagta actccaggta taggggtaaa acctatagtg gatgaagcaa atggaataat
     5101 aaaaagtgat ggtactacaa tattaggttc tgatgataaa gcaggtatag cagctatatt
     5161 agaaggctta agatatataa atgaaaataa catagaacat actgatatac aagttgtatt
     5221 ctctatatgt gaggaatgtg gattagttgg agctaaaaat ttagactatg gaaaaataga
     5281 ttctgaatat gcatttatat tagatagtgg tggttctcca ggtgaaataa tagtaaaagc
     5341 tcctgcacaa gatgtaataa atgttaagat attaggaaag actgctcatg caggtcttga
     5401 acctgaagct ggaataagtg ctataatggt agctgccaga gcaatagaaa atatgaattt
     5461 acttagaata gacgaagaaa caactgctaa cataggaatt ataaacggtg gaacagctac
     5521 aaatatagta acaggagaag ttaatatagt tgcagaagcc agaagtctaa aagaaaataa
     5581 attagatgtt caaactaaac acatggtaga gacttttgaa aaagctgcga aagattttgg
     5641 agctcaaatt gagatagatg ttaatagagc atacacacca atagatgtta gtgaggattc
     5701 tgaaataata aaacttgcaa agaaggcttt ttcaaactta ggtatagaag gtcatactga
     5761 atctacagga ggaggtagtg atacaaatat attaagtaag aatggtatcg aagctataac
     5821 tttaggaatt ggtatgaaaa atgctcatac actaagtgaa catatagcta taaaagactt
     5881 atatgattct gctaaaatgg tagtggagat aataaaggaa gcataaattt aaataatagt
     5941 gtataatata tatatcgcct agcttaagct aggtgatttt tcattttata gaagattgat
     6001 aatctctatt gaaagcaaag agtatacatt tgcatataag agagtgttta atttatgttc
     6061 aaatgtaggt tcttaataat atttagattg tttcataagg gaggaataaa tatggaaaca
     6121 aggtatgaag aagcaaataa aataacaata caatcaatac tttggaatat agtattaaca
     6181 ataattaagg ttatagctgg tgttattggt aattctagcg ctatgattgc ggatggatta
     6241 cattcagcat cagatattat aagttctata ggagtactaa taggcaatta tgtatcttct
     6301 agacctggag atagagagca taattatggt catgagaaag cagagacatt agtatcattt
     6361 gtgttatcaa tattgcttat tttcgtttct ataacaatag gtatagaggc aattaaatct
     6421 ttatttaact tagatgcatt aagtgttcca tcaatattac cattagtagt atcagttatt
     6481 tcaatcctga taaaagagta tcaatataga ataacaataa aagttgctaa aaagataaat
     6541 tcaccagccc taaaagctga tgcttggcat cacaggtcag atgctttatc ttcagtagct
     6601 gcttttatag gtataggtgg ttctatttta gggtttaaac cattagaccc aattgcgtca
     6661 gttgtagtgg ctatatttgt agctaaagta ggaattagta tacttataag ttctgttaat
     6721 gaattgatgg atgtttcagt tgatgaagaa gagataaaag aattaaaatt tattgtagca
     6781 gatacagaag gtgttaaaaa tcttggtgat ataaagacta gaaaacatgg agctatggca
     6841 tatgtagatt tgactatatg tgtggatgaa aatttgacag ttaagcaagg tcatgatatt
     6901 gctaccaagc ttgaaaaaca tataataaaa catatggaat ttgtaaaagg tataacagtt
     6961 cacgttgaac catgtactaa ttgccaagga aataagtgta ataattaatt aaaacttaaa
     7021 aaaaagtgta aaaagttata taattgtttt atgattataa actctagggg tggttaaatg
     7081 agcaagaaaa gaaaactaaa gaaaaaagct gcaatattaa taggagtagt tgttttcttt
     7141 ataatatact ttatatcctt tagaatagga ataaatatgg aaaaggataa aagtgaagtg
     7201 ccaaagaagg tagaaaaaga agaagaagat aataggtctt taaggactca aataaaagag
     7261 aagaagaagg tttatatatc agataaaaat atagaaaaca taaaaattga agaagatatg
     7321 tgggatgaaa taaagttatt tttctcagat tttaagaaag taagaaatac tacttcttct
     7381 tacgaagcag tttatgaagg ttattctgat gatggagtta aattttctac tgaccttaac
     7441 ttttttagag tatatactgt aaataaagaa gaatattata aagtaccagt tagttctaag
     7501 gatgctttta agaaactttt agatgagagt atatatactt cgtttgacct tttaagtcat
     7561 tataaagatt ggaaagaggt tactataact catggaaaag aaattaaaaa agttcataga
     7621 tggaaatttg atgatttagc acataagatg atttcaaaga gggttgtagg aaaagttcaa
     7681 cctgagaaaa gtaaggaaag aagtgactat aattttacta taaatataaa aggtgataat
     7741 tttgatgtaa aagttgatac aatgggaaaa gattatgtaa aagttacttc taagaaaaat
     7801 gtatcttact atgaggttca tacaagttta tttgattacc ttaaaaatga gatatttgaa
     7861 attggaaaga aataagactt taagaaggct atcaaattag atatttgata gcctaaatta
     7921 ttttatatat tatttttcaa ccaacaagtc tgcaacctta taaattggac cagcaccagc
     7981 agttattact aaatcattat ccttaacatt ttcacgtaag tattttacta tatcttcaaa
     8041 ttcttttata tagatggcat ctacattgtt ttgatataat ttctcaacca agtccttaga
     8101 atgtatatct cctgggtctt tttccctagc agcatatata tctgttatga taactttatc
     8161 agcagcatag aatgcctctg aaaattcacc taaaagagat tttgttctag tataagtatg
     8221 tggctggaag atacaccata aagttgattt ttttaatttt ttggcagcag ctaaagttgc
     8281 ttttaactca gttggatgat gtgcataatc atctactact aaagcattct tatagtaccc
     8341 tttagtttca aatcttcttc cgacaccttt ataaattttt atattttttc ttatagtctc
     8401 taagtcaata ccagaaacaa gagctgccat tatggcagaa gaagcattgt atatgttatg
     8461 aagaccatat acagaaagtt caaattctcc taaatctctt ccttcatatt ctattctaaa
     8521 tattccatga ccactattgt caaagtgaat atcttttatt acagcatcat tgtcagaatc
     8581 tctgccgtat tttataatag tagctttaac atcatacaat atgtcatcaa tattttcatc
     8641 atcgccattt attatgaaat agccttctgg gggtaataat tttccaaatt tgttaaaaga
     8701 agccttgatt tcatctattc cagagaaata atcaagatgg tcttcttcaa catttaaaac
     8761 tatagatatc tttgggttaa aattaaggaa actatctaca tattcacatg cttcagttat
     8821 aaagtggttg gaattaccta tcttaacatt accacctatc atacttaagt taccaccaac
     8881 tagtatagtt gggtctaagt ctgcatattc aaagattgta gataacatag atgttgtaga
     8941 agtttttcca tgtgttccag aaactgctat agaattttta tattccctca tgatttggcc
     9001 taaaaaagtt gctctgttca ttaccaattt atttttttct ttagcagcca caagttcttc
     9061 attgtctgga tgaactgctg ctgtataaac aaccatattt acatcatctg atatattatc
     9121 ttttttctga ccaatataaa tagttgcccc ttggtcccta agtttgtcta ataggtatga
     9181 ttcgtttgaa tccgaacctg aaacttgata acctttattg atacatattt cagctaatgc
     9241 actcatgctg ataccgccaa ttcctataaa gtgtatattc ataataaaac cacctttctt
     9301 tttaattaag tcctacaata tgaactattt ctgtggtata ataatataat gttcaatatt
     9361 ttataattaa tatgcatata atattatatg tattttattt ttgaatataa taaaacataa
     9421 gtaatattat accacaataa gaattgttat gcattagtta gtttaacgtt atataaaggt
     9481 acttgtagga ggaaataata atgaaattta aaagaacaga gagaattgga gcaatagtta
     9541 aaatactgtc tgataatcca aataaaatat atactttaag ttattttaca aatcaattta
     9601 atgcagcaaa atcaacaata agtgaagatt tattagttgt aaaaaatgta tttgagaagc
     9661 tacatttagg taaagttata actatttcag gagcagcagg aggagtaaaa tatataccta
     9721 aaacttctat agcagaaaat gcagagttct taatggaatt atgcgagaga atatgtgaca
     9781 agtctagaat actatctggt ggttttttat acttgattga cctaatttac gaccctacaa
     9841 tagctgctaa gataggaaag atattcgcat caaatattga gtatgtagat gcagattatg
     9901 ttgtgacaat ggaaacaaaa ggtataccta tggcacttat gactgcaaaa gctatgaatt
     9961 tacctctagt tataataaga aaagatataa aggtatcaga aggtcctaca cttagcatga
    10021 catatgtgag tggaaacagt tctaaagttg aaagtatgag tttgccaaga aaagctctta
    10081 aaccagatag taaagttata attatagatg atttcatgag gggtggaggt actataaaag
    10141 gaatggtaga ccttatgaat gaatttggtg cagaggttat aggtactgga gtattcatat
    10201 ctacaacgaa tccttcagaa aaaatggtaa aagattatat ttcattgatt caattggatg
    10261 ttaatggaga taaaatagta gtagaaccaa acttaaagac ttttaaagat gaatatagaa
    10321 atgaagattt agacgaagaa gaagattcag aatttgaatt tgagatagat gaagattaat
    10381 aaaaaaaata tttagaaaaa tatgtaaaaa taaaaagagg atatccctag ttgttcatag
    10441 aattatttaa aaaacagttt atattaattt attccagcga taataatttt aatataaatt
    10501 aatttctgtc aaaatcaaag ggggatattt tggtatgaag ataactgacg taagagtaag
    10561 aaaattaaca gaagaaggta agatgaaatg tatagtttca ataactttcg ataatttgtt
    10621 tgtagtgcat gatataaaag ttatagaagg ccataatggg ctatttatag caatgcctag
    10681 cagaaaagta ggcgaaggaa atttcagaga tatagctcat ccaataaatg cagaaatgag
    10741 acaagtttta gaagatgcag ttttacaagc gtatcatgaa gcattagttc aatgggaagt
    10801 agctgctgaa taataataaa tatataaaat ggagagagcc cgaacattta ggctcttttt
    10861 aaatgtagaa tttctattaa aatatgtact ttttagtaaa aaagtgctat attattagtg
    10921 ataatgtata tgttgtatac aaattgttag ataaatgtat tataatgtat attgattaag
    10981 agaataatac gaataaaatt aaattagatt gaatggagtg tatcaatgaa ctttaaagct
    11041 ataatccttg ctgctggcaa aggaacaaga atgaagtcta aatacccaaa agttatacat
    11101 aaagtatgtg gaaaagaaat ggttaaccat attatagatg tttcaaaaaa atcaggggta
    11161 aaagatactg tcgtgatttt aggacatgaa gctgatgtag ttaaagaaaa gcttgcagaa
    11221 gaaattataa tagccatgca aacagaacaa cttgggacag gacatgctgt aaagatggct
    11281 aaagaatata ttaatgatga agatacaata gttgtacttt gtggagatac tccacttata
    11341 aaagaagaga cactaaagag actatttgaa tatcatatag aaaataaata tcatgctaca
    11401 gttcttacaa caagagttgg aaatccaaca ggttatggta gaataataag agataagaag
    11461 ggtgaccttc ttaaaatagt ggaacaaaaa gatgcaaatt cagaagaaaa gatgataagt
    11521 gagataaact ctggaattta ttgttttaat ggaaagagtc ttagagaagc actagatttg
    11581 ctaaataata acaattcaca aggtgaatat tatttaactg atactgctaa aataatgaga
    11641 gataaaggat taaaagttgg tgcatttgca ggttcaacta ttgaagaact tatgggcgtt
    11701 aattcaagag tagaattatc aaaggcagaa gaaattatga gacgaagaat aaatgaatct
    11761 catatggtaa atggagtaac tataatagac actaactcta cctatataga atctgatgtt
    11821 atgataggca atgatactat aatatatcca ggtgttatgt tacaaggaaa aacaaggata
    11881 ggttcagatt gtataattgg aatgaattca tctataacaa attcagaaat aggtgatggt
    11941 acagaaataa aaaattcaac tataattgat agtaaggttg gagaaaattc tactgttggg
    12001 ccatatgcat acttaagacc aaagagtgac ttaggaaata atgtaaaaat aggcgatttt
    12061 gtagaagtaa aaaatgctat tatagaagat ggctcaaaag catctcacct ttcttatata
    12121 ggagatgcac atgtcggtaa aaatgtaaat attggatgtg gagtagtatt tgtaaactat
    12181 gatggtaaaa ataaatttaa atcaatagta aaagacaatg cgtttatagg ttcaaattct
    12241 aatttagttg caccagtagt tgtagaagaa aaaggatata tagcaacagg ttcaacaata
    12301 acacatgatg tcccagatgg tgctttggca atagcaagag aaagacaagt tatcaaagaa
    12361 ggatgggtag aaaaaaagaa tcaaaaagac gaccaaagta agtaattatg tagtttatta
    12421 ttttggctaa aaaatttagc tcgaaattat tataaattat attcattagt attttaaaat
    12481 tttcaggagg gttacataaa tgaatactag cggaagcgag attaaaatta ttgcaggtaa
    12541 ttcatcgaaa gagttagcac aaaaaatagc tgactatata ggtgtatcag tactagattg
    12601 tgaagtaggt acatttagtg atggtgaaat atgtgtaaac atgaatgaga cagtaagagg
    12661 ttgtgatgtg tttgtagtgc aatctactaa cagcccagta aatgataact taatggagtt
    12721 attaatctta attgatgcgt taaagagagc atcagcaggt agaataacag cagttattcc
    12781 ttactatgga tatgcaagac aagacagaaa ggctaaggca agagatccaa tcactgctaa
    12841 attagttgca aatttaataa ctgctgctgg agcagataga gtattaacaa tggatttaca
    12901 tgcagctcaa atacaaggat attttgatat accattagac cacttactag gtggaacaat
    12961 attagcaaat tattttaatg aaaagaaaat agaagattta gtagtagtat ctcctgactt
    13021 aggaagtgtt actagatcaa gaaaatttgc taatacttta aatggagaag ttccaatagc
    13081 tataatagat aaaagaagac cgaaagctaa tgtatgtgaa gttatgaact taataggtga
    13141 tgttaaaggt aaaaatgtta tactattaga cgatatgata gatacagcag gaacaatagt
    13201 taatgctgca aatgcactta aagagtttgg agcaaaagat gtttatgctt gttgtactca
    13261 tggtgtgtta tcaggtccag ctattgaaag aattgctaat tcagaaataa gtgagttaat
    13321 agttcttgat actatacaac ttcctgaaga gaaaagaata gataagataa agataaaaac
    13381 agtagctcct ttatttggag atgctataag aatgatattc tctaatgaat cagtaagtaa
    13441 attattctaa tttattgtat ttatgagagt tgtttaaaag gatataaacc ttttgagcaa
    13501 ctcttttttg attttatact tatggaatgt tatttttttc ttgttagaaa ggtatatatt
    13561 attgtaaaaa gagattatag tttaaaatta tgtatattta agtaaaataa acctaataaa
    13621 gggtatatta ttcaaaatat tatgaatata aaaaatatta caaaaaagta ttgaaaaaat
    13681 tcagattatg tagtattatg taaatataaa taaagaaaga ggtgtttcca atgggaaggt
    13741 tagaagatga acccctaata attaaatgat tcagtcacaa tctaaagtga atataaaaat
    13801 ctattgaaag tttatttata agaattgaaa atagtatcta ttctatcttt agtgtttgtg
    13861 agagggttct ggattatata agattactgt aaagagtaat tttgcacaat aagcatatct
    13921 gtaaatgaag agtttcgttt atataatgat tgtggctgaa tcataactat aatagataga
    13981 atgtttataa tagatgattt tataaaatac tatattgtaa tagcttttag ctttcaaata
    14041 aatattagca aaggatggaa atgacaatga taaaactaaa tgataattta taaatatctc
    14101 cttacgatat ataataattg tagggagatg tttctttttt tgtatagcat aatttttcta
    14161 gaaattctgt ttcaatatgt aaaaagttgg tatatataaa tagtataaac agtgttgcta
    14221 aaatatggta gatattatat aatataagtg gatatgtata tacattatga gttatataat
    14281 ttgaatttaa taaaatagaa acgttgattt atgttctgta atgtgggcac cttggagcat
    14341 attgagttag tggtgcaacc ggctatgaaa ttgtatttat ttataaatac tattattttc
    14401 atagcttttt ttatttaaaa aatggttata tgttagaaat aaatttggtt agtacgttta
    14461 aataatttta cttagatttt taaccagata tattgtaagt aagtattaat taaaaaattt
    14521 aagggggaat aaaaatgaag ttaaaaaaga ataaaaaagg tttcacttta gtggaattat
    14581 tggtagtaat tgcaattata ggtatattag cagtagtggc agttccagct ttatttagta
    14641 atataaacaa ggctaaggta gcaagtgttg agtctgatta tagttcagtt aagagtgcag
    14701 cattatctta ttattcagat actaataaga taccagttac accagatggt caaactggtt
    14761 taagtgtttt agagacttat atggagtctc tgcctgataa agctgatata ggtggaaaat
    14821 ataaattgat taaagttggt aataaattgg tattacagat aggtacaaat actgaaggag
    14881 ttacattaac agaggcacaa tcagcaaagt tattgagtga tataggtgaa aataaaatat
    14941 atacaagcgc tacaacaaat agtttgggaa atccattaac aagtaataca aaaatagata
    15001 ataacgttct atatatagta cttatagata atactgtgat ggacacaaca aaatagttaa
    15061 aacaatattt ttataaaaat attgtaaatt taatgaaaaa tataatataa aagacaggaa
    15121 gaaaatcttc ctgttttttt atactatttt ctttaatgtt caattatgaa tgatgatata
    15181 attgagatag taaaggagtg aaacctgtgg ctaaaaaagt tagaatagga gacaaactgg
    15241 tcgaaaaagg atatataaca gaagaacagc ttaaatgggc actatcagaa caaaaaaaca
    15301 gtggaaaaag actaggagaa tttctagtac aagaagggct tatagatagt aacttactaa
    15361 taagtgtatt aaaagaactc ttggatatag aaagtatatt tttagaaggg acagaaatag
    15421 acactttagc tacaaaaatg gttccagaaa atatatgcaa aagatatact gtatttccat
    15481 ttaaaattga tggaaataaa atatgtctag ccatgtcaga cccacaagat agagaagcag
    15541 ttcaagatgt aaggcgtatg tctggtaaag acgtagagat atttatatca agtacagaag
    15601 atataaacaa agcaataggt catgcttatg cacattcaga aataaataag gcaatgactg
    15661 agtacaacaa aaatagaact ggtggagtaa gagaaacagt aatacttgaa gaagatgtaa
    15721 atgctgctcc aatagtacga ttagtaaata acatactaga aaatgctgtt agaatggaag
    15781 caagtgatat acatatagaa caaagtgaaa attacatgcg tgttaggttt aggatagatg
    15841 gaatgttaag agaatatatg aggatgaact cagcaccata taaggcagta atcagccgta
    15901 tcaagataat gtcagatata aatatatctg aaaagagaat accacaggat ggtagaatct
    15961 atttaaaagt agataataaa cccatagatt ttagggtttc tacaatgcca actaacaggg
    16021 atgaaaagat agctatgaga gttcttgata agagtaattt tatggttagt aaagaagtac
    16081 taggaataga cgaacatggc tctaagatat atgatgaatt gataaacact ccttatggtc
    16141 ttatattggt tgttggccct actggaagtg gtaaaaccac tacattgtat tcaatgttaa
    16201 atcaactaaa tacagaaaat agaaatctat taacaataga agaccctata gagtatgaat
    16261 tacctggtgt aaatcaatct caaataaatg aaaaggcagg gcttactttc gccagcggac
    16321 ttagagcttt tatgcgtcaa gacccagata taataatggt tggagagata agagatactg
    16381 agacagcaga aatagcaatt agagcttctc ttacaggaca tttagtttta agtacattac
    16441 atgcaaatac agcagttgga gcaatttcac gtcttttaga tatggatgtg gaaagttttt
    16501 taataacttc atctgtttta ggtgtaatat ctcaaagact tacacgaaaa atatgtgagc
    16561 attgtaaggt ttcatatgaa gcagatattg gagaaaaaaa ggctcttggt attgatgtaa
    16621 atgaatcagt aactatttat agaggaaaag gttgtgaaag gtgtaataat actggatata
    16681 aaggacgtct aggtatattt gaaatgcttg aaattactcc agaaataaaa gagttaatag
    16741 attcatctgc aaatcaaaga gaaattttaa agatggctag aaaacaaggt atggttagct
    16801 taaaagagga tatagttaaa aaagttttaa atggaaagac aacagtagaa gaaatgatta
    16861 gaataatttt gatgactgat taaaggatga tattatgaaa agttttaaat acacagtaat
    16921 aaaagaggac ggaaaaaagc aaactgatgt gattgaagcc aacgatttta atgaagctag
    16981 aaatcttctt agaaagagaa atcttagagt tattgaaatc aaagaaagca aaaataaaaa
    17041 tataagatta tctagtagaa agagaaaaaa agacttagga gctgaccaga taagccactt
    17101 ttgtagacag tttgctatta tagtatcttc tggtataaat agtatttctg gattagaaac
    17161 attagctaga aggtcaacta acattacatt aagagaagaa attaatcgta tagtggcaga
    17221 aataaaagta ggttctacta ttgcagattc aatgctttca tcaaaatcaa agtttccaaa
    17281 gttacttggt gctatggtag ctacaggtga agctacaggt aaattagatg aagtattaaa
    17341 aagtatggct tcattttatg caagtgaaca cagggttaaa caaaagctta gaaatgctgc
    17401 aacatatcca ttaatagttt tgatttcttc atttataatg atttttatat ttacaacatt
    17461 tatggtgcct aagatgatta attcaattac aacagtagga gcaactcttc cacttataac
    17521 aagaattgtt atggggtttg gtatgtttat gaagaagttt tggataatag tacttttagt
    17581 tattatatta tttatctatc agtttaaaaa atatctaaaa acgtcagttg gaagggctca
    17641 taaagataga gttataaata agatacctgt tttaggaaaa ggaataaatt gtatggtagc
    17701 tacacgattt tcaagagcat tgtatctatt tgtatcaaca ggatacccat tagttcaagg
    17761 gcttgattat attatagata gtgtaaataa caccatggca gagaagattt tagcatcagc
    17821 taaagatgga ataacaagag gagaaggctt agcagaaaat ttagagagat atacgtattt
    17881 tgactctgtg ttggtacaga tgatagctat aggggagcag acaggggaat tagagaatat
    17941 aagtagacag atggcagaat tttatgagta tgaatccgag atttatttaa ataggatggc
    18001 ctcaatgata gagcctgttt taataattgc agttggtatt atagttgcga tactggttgt
    18061 atcaatattc atgccaatgc ttagtatata tgatgccatg taggagggaa aggacaaatg
    18121 aacaaggttt atgtatcata ttatggcgat tctatttcca tagttgaggg cagatataaa
    18181 cacgataagg ataagtttta tgtaaaaaat tttaatgtgt tttcaataga ggatattgca
    18241 caagattttt ctaaggaaaa gtatgctcta ttgagatacg cattagaaaa tgttaagtta
    18301 aaatcaagac atgtagtttt ttgtttaaat acaagagatg taattttaaa accccaaaaa
    18361 cttcctaata tggacaaaaa ggatttagac ggatttatga agctagagat ggacgaaatg
    18421 atggctttgg agtcagatga atatgtattt tcgtatgaag tatctagtga aacacctgat
    18481 aattcagatg gcgatgaatc aagtttaaat cttattattg gagctataca aaaggaagag
    18541 gtaaatgcaa ttgtagagat aatgcatgaa tttgatttag tattagatag aatagataca
    18601 ttatcaacag cttacttaag aatgcttaaa aatttagact acaatgatat aatggtaacc
    18661 aatataagtg acaatagcac aattataaat atatataaga aagatacttt atttattagt
    18721 gataatatac ctataaagat aactgctaaa gatatggaca tgcaattatt aaatgtagta
    18781 gaagaatcaa aaggtcttat gaattactat tcgtcaagaa actttggtaa ggttacggat
    18841 actatattaa tattaggaaa aagatattcc aataaattaa tttatgaaac ttttagctct
    18901 gcattttcaa gctatgtttc tcaaggcctt actgagataa tggatatatc tgatatgata
    18961 actggagata tagatgaaga tgatataaat tcaattgtag agcttttagg aagtatgata
    19021 gaacctaaag ggaaaagaga ttttgaaaat ataaactttt taccaatgga tatacttaga
    19081 aaacagataa aagctaaaca aatgaaaaac atactgaaaa ttgctccaat agttattttg
    19141 attcttgcta taccttattt atgtttaatt ttttcaaata atttagcaaa taaggaactt
    19201 gaaaatgtta atggtgaaat aagaaatatt gaatctgcat attatcagat tggaaatata
    19261 gaaaaaatga tacagagtaa aagcgaagaa ataaaggtat atgatatgtt aattgggaaa
    19321 aaggtaaagt gggaaaattt attggatgat atagataaaa atactccaag taaagttgaa
    19381 ctaacaagtt tatcaacaac ttatcaaaat cttggaacta ataataaagc tgataattca
    19441 aaaagtaata agactacaac agataaatca agtaacaatg aaacaaaatc aagtacgaaa
    19501 agtgatagta atggagataa aacagataat gatttaaatg aagataagac tacaaatgtg
    19561 tcagaagcat taactggaaa tagtgagact aatagtaata acacagataa agatgataaa
    19621 actccattgt atgataagat accaaatgtt ataaatatag aaggaaatgc taaagattcc
    19681 tcttatgtag gtcaattttt atataaatta aagggattat actatttcaa ggatgttaaa
    19741 ttacatagca taaaaagtaa tgcagaagat tctaactata catttagtat gacattatac
    19801 ctgaaagaag gtgtattaac taatgaataa agatatattt aaaaaagata taaaagatat
    19861 atttaaaatg gaagttagtc taaaagggat tcttataaag gttataattt tattagctat
    19921 tttatcatat ggttattttg gactttaccc tcaatatcag aaatatattg ctacaaaaaa
    19981 ttctgtaaaa caggctactg aaaaattaaa tatatataaa gagaaaactg cacttatgcc
    20041 aaaatatgaa aaacaacttg aagaagttga aagtgaatta aaagaaaaaa gcaaaaatct
    20101 tttatatgat atgcaagatg gtcttttttt aataggttta gataaaaaaa tgaaggctta
    20161 taatattgac ttaaccagtt atgacgttgg aaatgttaaa gaatacgaac atttttatgg
    20221 tatatcaaca acaataagtg ttagaggtga ttatagaaat gtcaaaaaat taatggctta
    20281 tttagaagaa caaaaaaatg ttactcaaat actagattat acaatgactt catatataga
    20341 accagaaaaa gaagaggaag aaacaacaaa caataaggat ttaattaaaa atgtgaacat
    20401 aaataaagat acaaaaatat actgggtgga aggcgaaaat aagtggcatt tatataagga
    20461 ttgtccattg ataaaagagg gagagattct aaaaaatgac aaacttttag ataggtctac
    20521 taattatcta tgtaatactt gtgtacttag agctgcaaca gaaaaagaag caaaaaaaga
    20581 agtcgtagct ccaaaggcag aaggtgtagt agatgctaca tttaaattta taatgtacac
    20641 taaagagaat actacaatga tacttgatac agataatcca tcttcatgga gaccaggaaa
    20701 atataatcca tttaagtcta gtgcgaaata aagctaagta agctttaaag ataggtgatg
    20761 agtttgtaca aaaataatga aaaaggtctt actttattag aagtaataat agctgttttt
    20821 atactgacaa tagttttaag tatttcttat aaagtgttca atggtataac atcagcagta
    20881 aaaaagcaac agattattac agatgctcaa gtaaatatta atctaattaa taagtatcta
    20941 aatagagatt tggaaaactg taaagaacta actaaaactg gttcaggtaa taattatgaa
    21001 tacaatatag agatgccaga taatgttgta aaatatgaag tttctataga aactaaaaaa
    21061 aatacagagg tatattctgt aacgagaatc caaaacaata caattgatac agaaaatgaa
    21121 gttagagaag agataatcta taatcaacca ctagttcaaa acaataaaga aatgaaggaa
    21181 acaccattta aaatagaaaa gcaaactggt aaatctattt atactgtaag tatatactat
    21241 aatgaatcag tgcaagaaag tcataaaaat agcaatttaa ataacaaaac ttatacattt
    21301 gatgttatgt caagaatagg ataataagaa tttattaaaa aataagtagg ttgataaaat
    21361 atgagtaaaa agaatagtga aagaggattt tccttaattg aagtgttagt agctatggct
    21421 attatgggaa ttgtactatt tgcatttttt aatattatca atactaataa caaagcaaac
    21481 actaaaaatg acacagatat aacttcctta aactatgtcc aaagtgaaat agaaaatcta
    21541 agagaaaaga taaaaagtgg agaatttgat tttgatagtt tagataaact agaagatgga
    21601 actgtcgtat atgaaaaatt aatagataaa tcaaaaaaag ttgtatatga taaagttctt
    21661 agtgaaggtg atgtgagctt atatgatact ccatatgaaa agattacaac aataaaagat
    21721 gaagatggta atttaatcga caaaggaaat attacaaata agataaagac tattgtagaa
    21781 gataaaagtg gtcagatata taaaatagct gtaactggga aaagcatgaa tgactattca
    21841 agtaaaaaag aggttaaaat tgtaactgaa atatttaaag ataaataatt ttacttgggg
    21901 gtgagatttt gaggaagtgg aataaattta aaagtgaaag aggggcagct ttagttttag
    21961 ttttggttgt agtagcattg ctttctattg ttggattgat attttcaaat cagatagcta
    22021 atagaataaa atctacaaag actactaatg aaggtataca agcaaagtat ttagcagaga
    22081 catgtgtgga aaatagtatt gataaagcat atgaaaaatt gtatgatgag cttgagaaaa
    22141 tggataatga gtttaaaagt gaaaatcaag aaaaatccat atctaggtca aaattaagag
    22201 atatatcaga tgaagatttt aataatcaag atgaggaaaa tattgaagca gaaagacttg
    22261 gatatatgaa taatataaat ttctatttaa ataaagcttc ttcagacttg gaaaaagcta
    22321 gtatggaatt gaaaaagcta tatgatttag atatgttgga ctatagagac attgaatatg
    22381 tagatgcaaa tattatttca catagagatt ctatcttaga aatttgcaag aattatactt
    22441 ctggagatat tagtaaaata aatgaatata ttctgaaaga agatatagac tcaactacat
    22501 tgattgaggc aaagttagtg aataatgata ttcttttaaa aatgttttta gaagaaaata
    22561 aaattgaaaa tgaacattta aattcagcct tttcacatac ttataaggca ttagataaca
    22621 tttctttggc gatgcaaaat atgattgagt atagacatac gtttcatata gatgagccaa
    22681 aagtagaagt atctaatggg attccagatt ctcaacaata ttatgaacta atacaaaatc
    22741 ctataataaa tagtatggag tatatctgga atagtaaatg ggatacatta gaaaaactat
    22801 tggaaatttt accaaatcaa actcaaggat ttaattcttt aagagttcat ttgagaaata
    22861 atgtaagaaa atttgagaag ttaagcgata atatatctag tggtaaaaaa aatacagcaa
    22921 aaaatttctt aaagtataaa gagttattgt atgaaatatc agaccaatgt aatcaactaa
    22981 aaagtatgtc ttatgagaaa atacctgtta aatatgataa catggctttg ataacaactt
    23041 ttgactatat acaaaatgag cttttagcag agataaaatg cagattaaaa gaattaaaac
    23101 cacaagaaat tgataaaaca gaaggtatta ctataaaaat acctttttat aaggctgact
    23161 acgacatgac aaaagaagga tggcctaaat taaaagaaaa tggttcaggg gcggagttaa
    23221 gcttaatggt aactggtgat aaagatggaa taaaagaagt agaagttaca gatggtaaaa
    23281 aaaatattat tggtttaggt gttgaagaaa attcaaattc aaagtataag gttgacgcca
    23341 ttgtaaactt taatctaaat atagatacaa atgtagttgg aaattatgat ataaaagata
    23401 aaattttgat taatcatgat ataagttcat acaaaaaagt aaactaaata taagaagcaa
    23461 cttagtataa ataaataata aaataatttt attatttact actaaaaaaa taagtaaact
    23521 aatagaagat atggagagta aatattatgt atatttatga tttattagag caaggtataa
    23581 gactaaacgc atcagatata cacataacag ttggaacaaa tcctgtagct agagtaaaag
    23641 gtggatttgt aaaactaagt gaacaaatat tgacatcaga agtaacaatg caaatggcaa
    23701 aagacatagc aggggaatct atgtttaaag taatagaaga acatggagaa gctgattttt
    23761 cagcttcttt aaaaacagga gaaagattta gggtaaatgc atataggcaa aaaggaaatt
    23821 acgcaatagc aataaggact ataacagcag aaattccaac atttgaaaaa ttaggccttc
    23881 cagagagtat aaaatctttt actgaaaagc ataaaggatt agttttagtt acaggtccaa
    23941 caggaagtgg taaaagtaca acacttgcaa gtatgataaa tataataaat gaaaaacaac
    24001 aaaaacatat aattacactt gaagacccga tagaatatgt acatcatcat aaacaaagtt
    24061 tagtaaatca aagagaagta ggaactgata cagaaagttt tcattcagcc cttagagcta
    24121 ttttgcgtca ggacccagat gtaatactaa taggagagat gagagaccct gaaactgttt
    24181 caatagcatt aacagcagca gaaacaggtc atttagtatt ttcaacacta catacagtgg
    24241 gggcagctaa gacaattgat agaatagtag acatgtttca accatcccaa cagcaacaaa
    24301 taaaaacaca gttatctaca gtttgtgaag gtgtggtatc acagcagtta cttcctacag
    24361 cagatgggaa aggcagaata gcagcaattg aattgatgtt tgctactcca gcaataaaaa
    24421 acctgataag ggaaggcaaa acatatcaaa ttccaaacat gatacaaact ggagttaaag
    24481 taggtatgaa aactatggac caagatttaa tggaacttta taaaaatgga aagattacaa
    24541 aagatatggc acttagtagg tgtactgacc aagagtttat gacaagaatg ataggtggag
    24601 taaactacaa tggatattat aatcgttaat ctatatgtat ttatggtggg tatagtgttt
    24661 ggaagtttct ttaacgtctg tatttttagg attccagaag gtatatctat ttctagacct
    24721 ccttctcatt gtatgagttg taatactaga ctaaagccaa tagacttaat accagtgtta
    24781 agctatttaa tgagtggcag aaagtgtaga tattgttatg agaaaatttc tagtagatat
    24841 gctatagtgg aattaattac aggactttta tttttaacag tatttatgtt atatggaata
    24901 tctacatcaa ctatttatta tttggttctt gtgtctttac tgattataat tacatttata
    24961 gatattgacc actttataat accagataaa atattggttt ttggagctat attttctatg
    25021 atttttaacc ttttatttaa agaaattcca gttaaggatt caattctagg tgtttttatt
    25081 tgtggaggaa gtgtctggat tatagttctt ttgattgaat ttgtaataaa aaaagagtgt
    25141 atgggtggag gagatatcaa actatttgca atgctaggtt tttacatggg tgttaaaaat
    25201 ggtcttttga cagccttact aagcgtttat gtgggagcag tctatggaat ctgtgtaata
    25261 attataagta gaataaaagg aaaggaatat aattctgtta taccatatgg accttttata
    25321 tctattggag cattaataac aatcttatgt ggaaagcaat tacttgaact ttatttttct
    25381 atggttatat agatgatagt caagttaaat aaagttttaa tttaaaaaaa tgaattaaag
    25441 tatttaataa ggacaatatt attattaacc ctattactaa agtgctacaa actatggagt
    25501 atataataat aatttcctta ttttttgcac atttgataaa taaatctcta tgtgtttttt
    25561 gtgatagaga aaagaaaaat ttatacaata cgttttttat ttacataata actgtagttt
    25621 tatctgtatt aatatatcta aaatttggga ttagtgtaga atgtgtacaa tatatggttt
    25681 taattccatt tttaataatt atatccattg tagattacta cacaatatat gtatatgata
    25741 taacaatagt tagtggtata atagttcaag gtataatttt tttggtcttt ttcaatatag
    25801 ttcaatttga ttatataaat catatatttg gactattgat aggctttatt ataccttata
    25861 ttattgctaa actaacaaaa ggattaggct atggagatgt aggtctctat agcttatgtt
    25921 gctttgcatt gggaaataat tatgggtttt atgtgatagt tttatcattt attttagcgt
    25981 ttttgttttt tattatttta tacataacta aaatatttaa agatgtagat ataagaaagc
    26041 aaattccttt tacacctttt atttccttgt caacaatttt aataatattt acagagtata
    26101 atatatttaa tttttattat aatttgttat acaaatttat ataacaggag gtaagaaatg
    26161 tacgtagtag tagggttagg taatccagga aagaagtatg agaagactag acataacgtt
    26221 ggatttgatg ttatagatat tcttgctaaa gaatacaata ttagtgttac taaaataaag
    26281 cataaagctt tgattggtga agggagagta ggtactgaaa aagtattatt agttaaacca
    26341 caaacttata tgaatttaag tggagaaaca ttaatagata tatataaata ttataaggtt
    26401 gatttaagta atatagtagt agtatatgat gatatagatt tagaagttgg aaagatacgt
    26461 ataagaaaaa aaggaagtgg aggaactcat aatggcatga aatctataac taagtgccta
    26521 ggttcaaatg actttccaag agtgagagta ggtgtatcta aaccagaagc tggtcaagac
    26581 ttagcagact ttgtactttc tagatttaga aaagaagaat ctgataacat aaatgaagca
    26641 ttagaaaaag cggcagatgc tattgactca ataataagag aaaatataga tatgtctatg
    26701 aacaaatata atggatagtg agagggagat ttaaatgaat gatgtttttt tatatccttt
    26761 gcaaaattca aaagagtata aagatataat aaactgtata aaaaatacaa aaggctcatt
    26821 attggttaat ggacttttac cagtacaaaa acctcatata tcgtattcta tatttaatga
    26881 tttatcacga caaatgattt ttataacaag tagtgattta gaagcaaaga aagtatacga
    26941 agatttaagt ttttatatgg aagataaagt tgaatattta ggttttcaag atatttattt
    27001 ttatcacttg gatgcaaagg acagaaacga agaagcgaag aaattaaaag tattgttaaa
    27061 actagcaaat aaagaaaaaa tcattttagt aacttctatt gaggcagttt taagaaaata
    27121 cataccaaaa caagttttgc tcgatagtgt atctcattat aaggtgggag atagcttaga
    27181 cttagaaaag ttaactgaga aattagtttc attgggatat gaaagagttt caaaaataga
    27241 ggggtttggt caatttagta taagaggtgg aattattgat gtattttcac ttgaatatac
    27301 aaatccaatt cgtatggaat tatttgatga tgagatagat tccatacgaa cttttgacgt
    27361 atattcacaa aaatcaattg ataaattgca acaattctct ataactccat ctagggaatt
    27421 tatttatccc gaaaaaacta cagatgcatt agttaaatta aaaaaagaaa caactaaaaa
    27481 tactgatgaa ggtacatttc aaaatataga ttatatatca agtaagactt attttgaagg
    27541 tgtagagaac tatatagatt atatttatcc agaagaaaac aaaagtatat ttacttattt
    27601 agcagatgat gcaatagtat ttattaatga tataacgaga cttaaagaaa gatgcgaaaa
    27661 ttatataaat gagtttagag aaaattataa attaaatctt gaaagaggtc tggctattaa
    27721 aaaccaaggt aaattgttat atcactatac agatttagag tacttagtaa aagacaagtc
    27781 tgtggtatta aactcgctat tgccaaagtc tataaataac ttttctataa aatctataat
    27841 aaattttgaa agtagagaag ttcctacatt taatggaaag gtagatttgc ttgtagaaga
    27901 attaaatcgg ctgaaatata atggatacaa aataatttta gcaacaaata ccttagaaag
    27961 agcaaataaa ttaggaaaag atttattgga caaaggctta gaaacgacca tttctaaaga
    28021 tagagatatt gaaattaaat cttcacaagt tataattgta cctgcacaca taaacagtgg
    28081 ttttcagtat aaatctataa agtttgttgt tataacagat aacgaaatga taggtgttta
    28141 taaaagagcg tctaagacta gcaataaaaa agttaaaaag ggaaagaaaa tagaatcctt
    28201 tttagattta agtgttggag attatgtagt ccatgaaaat agtggtgtag gtagatacac
    28261 aggaattgaa caaattacag tcaatgctat taaaaaagat tatatgaaaa tagtatatca
    28321 aggtggagat aatctatatg tgccaataga ccagatggac aaagtacaaa aatatattgg
    28381 tgcagaagta gaaaaagtta agttaaataa gcttggaaca aatgagtgga cgaaagcaaa
    28441 agcaaaagtt aaaaaagaaa ttgaagatat gacaaaagac ttgattgagc tttatgcaaa
    28501 acgtgaaaaa atacaagggt ataagttttc aaaagataca ccttggcagg cagagtttga
    28561 atcgttgttt ccatatcaag aaacagaaga ccaattaaag gctatagaag aaactaaaaa
    28621 agatatggaa tcaagtaagg taatggatag gctagtatgt ggagatgttg gttatggaaa
    28681 aacagaggta gctattagaa gtatttttaa agcttgtatg gaccaaaaac aagttgctgt
    28741 attggttcca acaacaatat tggcccaaca acattataat acattcaaag agaggtttga
    28801 aaattatcct ttgagagtag aagttttaag taggtttaaa acaccaaaac aacaaaaaca
    28861 gattattgaa gatgctaaaa aaggattagt agatatttta attggaacac atagaataat
    28921 atccaaagat ataaatttac caaacttagg tcttgtagtc attgatgaag aacaaagatt
    28981 tggagtaaaa cataaagaag ctttaaagaa aattaaatct acagtagatg tacttacact
    29041 ttcagcaact cctataccaa gaacattaca tatgtcttta agtggaataa gagacatgag
    29101 tgttatagaa gaacctccac aagaaagaca tcctgtaata acatatgtta cagagagtaa
    29161 ggaaagcgta attcaagatg agatagagag agaattaagt agaggtggac aggtattttt
    29221 tgtatacaat agagttgaac acattgaaga aatggcaagt atgattcaaa aactcgtacc
    29281 agatgctaga gtggctgttg cacatggaag aatgacttct aaatcattag aaaatataat
    29341 cctaggattt ttaaataaag attatgatgt actagtctgt actacaataa ttgaaactgg
    29401 tatggacata tcaaatgcaa atacaatgat tatatatgat gcagataaaa tgggattggc
    29461 tcaactttac caattgagag gtagggttgg aagaagttca agacaaggat atgcatatct
    29521 tttgtatgaa aaagataaaa ctttaagtga gatagcggag aagagattaa aagctatcag
    29581 agaatttaca gagtttggtt ctggttttaa aatagcaatg agagaccttg aaataagagg
    29641 agcaggaaat attttaggtt ctcaacaaca tggtcatatg gcagttatag gttatgattt
    29701 gtacgttaaa atgcttaatg atgcaattaa aaaagtcaaa ggtgagccta ttgtggagga
    29761 aattgatgtt gaaatagatt taagtgtaaa tgcatacatt ccagataatt atatcaaaga
    29821 tgagttgatt aaaattgaaa tgtataagaa aatagcttct attgaaaaca aagaagacat
    29881 gttggatata caagaagaac ttgaggatag attctctgat atacctaaac cagtccagac
    29941 acttcttaca atagcttata taaaatcgtt atgtaagata ttaaaaatag aaaagataag
    30001 acaattaaaa gatgaaatat tattagttcc aataacaaag tatagaacta aacagaaaat
    30061 aggttataat atagttacag aattagaaga attattagaa aagatgtgca aggtaaaata
    30121 acttgtaaat ttaagaaggg atgttttaaa tttgaaaaaa gtaataacat tggtaatagc
    30181 tatgattttg gtagtatctg tgactgcttg cagttctagt aaaggagaaa ctgtggcaac
    30241 agttgaaggt acaaagatat catcagatga atttaaaaaa actatagctc tttataaaga
    30301 ttctatggaa caaacttatg gaaaagatat atgggataag gaagttgaaa agggtgtaaa
    30361 atacaaggac aagtttaaag atttaattct tgaccaactt ataactacag aagtaatata
    30421 ttctcaagct aaaaaagata acttacttcc taaaaaagag gatgttgaaa aaagttttaa
    30481 agaattaaaa gacgctatgg gtaaagatga aaaatataaa gaacaactta agaaattagg
    30541 tatagatgat gagtttttaa gagaccaaca agagaaagat ttagcaatgc aaaactatca
    30601 aagtaacttt gctaaaaaga ctaaaatatc agatgaagaa atgaaaaaat actatgatac
    30661 acataaagat gaatttaaaa aagatgaggt agaagcttct catattttat tgaaaacagt
    30721 agatgataat aacaagccac tttcagataa ggaaaaagct gaagctaaga aaaaggctga
    30781 agaggctctt aaagaagtta aatcaggtga ggattttgca aaagtagcaa aaaaatattc
    30841 acaagatact tcagcaagtg atggtggtaa attaggattc ttttcaaggg gtcaaatggt
    30901 tgctgaattt gaagatgctg ctttttctat gaaaaagggt gaagtatctg atttagttga
    30961 aactcaatat ggataccaca taattaaagt gacagacaga ataaatgagc aaacatcatt
    31021 tgaagatgct aaagaaacta taaaagacca actgttgaaa aataaatatc aagaacaaat
    31081 agaaaaatta actaaagaag ctaaggtaga aaaagatgaa aaagtaataa ataaaatcac
    31141 aatctaagac ttaaaaaaag agtaatatgc ttaaaaaagc tatatctgga taagatatag
    31201 ctttttttgt atatttttct taacaatggt aaaaataaac ttaaagtaat ttatggaggg
    31261 attaaaaaat atgaaagcaa caggtatagt tagaagaata gatgatcttg gaagggtagt
    31321 tatcccaaaa gagataagaa agacattaag aattagagaa ggagaccctt tagagatatt
    31381 cacagcaaaa gatggagaag tcatacttaa aaaatactct ccaataggtg aattaaatga
    31441 attttcgcaa gaatatgctg aaacattagc tgaagtacta ggatatggag tgttgataac
    31501 agacttagat tctataatag ctgtgtctaa gcttcctaaa aaagattata aagaaaaaag
    31561 tataagtgat gagttagaag caataataag tgaaagaaca gtaaaatatt caaaagataa
    31621 aaacatttta ccattattca aggaagacca aaaagaatat actagtcaag ttatcatgcc
    31681 tataattagt gcatctggtg actgtatagg ttcaattgta gttgtttcaa aggataaaga
    31741 tacaattgca gaaagtgaag aaaaatcttt aaaaattgct gctaactttt taggaaaaca
    31801 agttcaataa ttttatcata aagacctttt acatgaaggt cttttgaagt ttttgtgtaa
    31861 aatattgaat atagcattaa aaatatggta tattaaagtt tgttatataa acagtatgga
    31921 ggttatgtta tgaacaataa caaaaataaa gactcctttc taaaaggagc ccttatactt
    31981 gggctagcag gtgtaatcgt aaaaataatg ggtggatttt ttaggattcc ccttggaaac
    32041 atgattggtt cagaaggaat gggatactat caggctgctt atcctgtgta tactctattt
    32101 ttaacattag ctactgctgg atttccaaca gctgtggcaa aattggtttc ggaaaaagtt
    32161 gcaattggaa attttaaagg tgcaaataag atatttaagg tatcacatac agttttattc
    32221 attaccggta taatatcatt ttgtatatta ttttttggag ccgactacat agtaacaaac
    32281 gtaatgaaaa atccaggtgc gttatactct atgaaagcca ttgctccagc attactattt
    32341 gttcctgcaa tgtctgcata tagaggatat tttcaaggga gacaagatat gactaagatt
    32401 gctgtgtctc aagttgcaga gcaatttttt agagttgtat taggtcttac tttggcttac
    32461 ttgcttatga agagtctagg tcagactttt ggtgcagcag gtgctatatc aggggctaca
    32521 ataggttcaa tagcatctat gctttatttg gtgtttgctt atatgctagg gagaaaagag
    32581 cgtagagcag agatagatgc aagccaaaga tttaaagatg aaagagtgtc ttatattttt
    32641 aaaaagcttt taactgttgc cattcctata acaataggag catcagtaat gccacttgta
    32701 aatatgatag ataatgttat tgtaataaga agacttatgg aagcaggctt tacatacaaa
    32761 gtggcaaatt ccatgtttgg tcaattaaca ggtatggcaa tggccacaat aaatttacca
    32821 gcagtcataa caactgcaat gagtatgagc ttagttccag caatttctaa agcatatgca
    32881 ttaggaaata agtcaaaagc tagaaaagat actaagagtg cagttaaggt aactttactt
    32941 atagtattgc cttgtgcgtt tggtatggct tctttagcaa taccaataat gggtctttta
    33001 tttccacatg aaccatcttc tgtaggaact attttgttta ctcttactcc atgtgtatta
    33061 tttttaggat taatacagac attgactggt ataattcagg gaatgggaaa accaatagta
    33121 cctgtaattg ctttatgtgt tggtatgcta tgtaaaattg taataagtta tactttaaca
    33181 ggtataccag atattaatgt actgggttca gcatttggta cagttacagc atactttgtg
    33241 gcagccatga ttaacttact atatgttaaa aaacacatga atgtaaactt ttctaaaaaa
    33301 gaatttataa taaagccatt tataacagtt atgacaatgt ttataatggt taagttgagt
    33361 tatggtgctt tagttggatt cttaggaaac agcatatcta caataattgc aatatgtgtt
    33421 ggtggaattg tatatgtagt agtaatactt ggaataggtg gaattaaaaa agaagagatt
    33481 ctaactatgc caaaaggtga taaattatat aaattgttaa aaaaagttaa acttataaga
    33541 taatatagga atatatatgc aaataaagga aatactatac aaatagtaaa tctccttatt
    33601 tgtataataa tataaaaata aagtgaaatt tgctaatagg agtgatgtta tgggtaagat
    33661 aagtattgta ggtcttggac caggagacta ttctcttata agccaaggtg tgttagaatc
    33721 tttaataaat agccctaaag ttttttttag aacagagaaa catccaataa ttgataaact
    33781 taaagaaact atacaataca cttctttaga ctacttttat gaaaaagagg aaaattttga
    33841 taatgtttat ttgaaaatat ctgaatttat catagaagaa tcagcaaagg gtgacctagt
    33901 gtatgctgtt cctggtcatc caagagttgc agagaagact gtaagtataa tagagagatt
    33961 agccaatgaa aagcacatag cagtagaaat attgccttct atgagctttg tagacgctat
    34021 gtttaactat ttggctatag acccatctga gggatttaaa ctggtagatg cttttgaaat
    34081 agaaaattca tatatagact tggatacaag tatgataata actcaggtat atgataaatt
    34141 tatagcatct aatgtaaagt taaaacttat ggaacattat gactataatc aagaagtatg
    34201 tatagtaaat ggagctggtg tcaaagattt agaaagtaaa aagtttataa agctttgtga
    34261 attagacaga aatgaaaact tttttgacta tttaactagc ttatatatac ccaaaagttc
    34321 taaaaagatg tataatacag tacatgactt agaaataata gttaatacac ttagaagtcc
    34381 atctggatgt gaatgggata aaaaacaaac acatcaatca ttaaaaaatt ctgttataga
    34441 ggaagcatat gaactttgta atgcaattga taataatgat atagatgaaa tggtagaaga
    34501 gttaggtgat gttcttttac aggttatatt ccattgtcag ataggaaatg aggagggcta
    34561 ctttgattta aaagaagtag taaatggcat atgtaaaaaa ttaattcata gacatcctca
    34621 tatatttagt ggtgcaaaat tagatatgtc taagtttgag aaaacttggg aagaattaaa
    34681 gaaagaagag aaaggcgaaa gtactataac agatggcctt agaaggatac caaaacattt
    34741 accagctctt atgaaggcta gtaaagttca acataaagca gcacttgtcg gctttgattg
    34801 ggacaatgta gaagatgtat ttaaaaaaat tgaagaagaa tatcaggaat tacttgacga
    34861 acacagggtc gggaatataa aatacataaa ggaagagcta ggcgatttat tattttcgat
    34921 agtaaatcta gctagatttt tagacataga ctcaggagaa gccttgaact gtactacaga
    34981 aaaattcata aatagatttg actttataga aaagagtgca atgaacttga ataaaaaatt
    35041 agaagacatg acacttgaag agatggatga gttttggaat caggctaaaa aacaataaaa
    35101 ttgtggaagg aaatttgtca aaaatctaga acttttatca caaagataga agtctatatt
    35161 ataaacattt ttaggaggta aatatcgtga ataaagctga attagtatca aagatggcag
    35221 aaaaaagtgg attaacaaag aaggaagcag aagctgcgtt aaacgcattt atgagttctg
    35281 ttcaagatgc actagtaaat aatgaaaaag ttcaattagt tggatttgga acatttgaga
    35341 caagagaaag agctgctaga caaggaagaa atccaagaga tccagagcaa gttatagata
    35401 taccagcttc taaagcacca gttttcaaag ctggaaaagg attaaaggat ataataaatg
    35461 gataattaga aatcttgtga aaaagtgtgg ataaatttgc cacacttttt ctatagaacc
    35521 aaggaaggtg ataattatga gattagataa atttttaaaa gtatctagga ttataaaaag
    35581 aagaactgtt gctaaagatg catgtgataa agggattgtc actataaatg gaaaagttgc
    35641 taaatcttct tcagaggtga atattggaga tacattagaa atacaatttg gagagaagaa
    35701 gatgaagttt aagataaatg aagtaaaaga gcatgtatta aaaaatgatg caaaagaaat
    35761 gtacgaaatt ttagaataaa attaatagtc ctctagtata ttaatagtag tattattgaa
    35821 atatttactg gggggatatt atggaacaga acataacttt gaaagataga tcaaagctgg
    35881 ttatttcggg agtcgagcac atatactctt ttaatgacaa aagagtagag ctcaaaactt
    35941 ctgtaggcga gatggtaatt gaaggcgaaa acctagatat gagcaagcta agtatagacg
    36001 aaaatataat tagcatagat ggaactataa attctatggt atatgcaaag cctaaaaagc
    36061 cacaagagag tttcttgaaa aaggtattta gatagatgat accttttact caagatgtaa
    36121 gcatttttta tgctactatt tatggtggaa tactcatagg ggtgttgttt gatttttata
    36181 gagggctaag agggaatttt aaattcatca attattttgc tataatattt gatgtattgt
    36241 tttggtttct agctacggtt ataatattcg taacaataaa ccttacagaa ttttttgatt
    36301 taagatatta tcattttgtg gctttgttta ttggttttat tttatactat aacaccataa
    36361 gtaaaattgt tttatcaatt atcaataaaa taattcgttt tgtgagaaat tcttttaaaa
    36421 aagttacaca ttacatagtc agttttttaa ataatttgta ttacgttata atctattcac
    36481 tacacctact atttgatatt atattttata taccaaacat atttatagca acaagaaaaa
    36541 gtataaaaag aaaatcaaat aaaaaattaa agaacaagaa aaaatcaaaa cctaagaaga
    36601 aaaaaaataa aactaaaaaa agggtgtagg aaaaatgaac ttaagaaaaa aattttcagg
    36661 acagattatt gttatcagtt tatttttagg aatatcaatt tttagtatga tgacaggatt
    36721 tgtatttgag tatacaaaag ctaaggaata taaaaaagaa atagcaagtc taaataaaca
    36781 attaaaaaag actgaaatac aaataaacag cttaaagaaa gatgaaaaat catatgaagg
    36841 agacttagag gatatagcaa gaaaaagatt gaatatggta aaaccaaatg aaacagttta
    36901 tgtagacata aatcgttgat taaaacttgc taataagttt acagatgtat tgattttaaa
    36961 atttaagatt taataaaaaa gtcattcagt taaggatggc tttttgtttt tattgaggag
    37021 ggaatttcat aatgaaagta ggagctatag atattgggac aaactcaatg agattattga
    37081 tagcagatta tagagatggg aattttataa acagaaaaaa atttgttaat acaactagaa
    37141 ttggttatgg tgtggatagt aaaggatata taaaagaaga tgctatcaaa agaaatataa
    37201 aagcattaaa agaattttct gataagtgta atgatgaaaa gtgtgaaaaa atttattgta
    37261 tggggacatc tgctcttaga gacagtaaaa atggagatta ctttgttaaa ttagctaaag
    37321 aagaaactgg aatagatgtt gatatagtta cgggaataga agaatcaaat ttgggcttta
    37381 agggtgtttt agaaggaata caaggtgatg atactattat tgttatagat attggaggag
    37441 gttcaacaga atttattgta ggtgattcag aaggaattat atttaataaa agtgagaatg
    37501 taggtgctct taggatgaca gagaggtttt tgaagcacga tattattgaa gatgaagagt
    37561 ttgaagctat gagttcattt atatatggtg agattgataa aacgttaaat catataaaag
    37621 acaaaggatt aaaaactctt ataggtatag gaggtacgat tacatcatta tcagcaatga
    37681 atcaagaatt agagatttac tctatggaaa aaatacataa tagtaaaata cacaaaaaag
    37741 aaatagaaga aattctacaa aaattaataa agatgacatt aagtgataaa aagaacttaa
    37801 aagggctaca agaaaaacgt gcggatatta taactgctgg tgtaaaaata ttgaatataa
    37861 taatggaaaa attagaaata gaagaaataa ccgtaagtga atacgataat ttggaaggct
    37921 taatatgtca aaagtcaaaa aaaatgtatt aatatttttt gatttttaaa accgattatg
    37981 acaaaaaaaa ttactcctct ttgttacaat tcttaatata aacaaaagag gaggtttttt
    38041 tatgcaaaga agtgtagcag ttgtgaagaa taagactgta aaaccaaatg tcataaaatt
    38101 aataagcgga tatattgatt ttactacggc ggcattcgct gtgatgggat ttttacttag
    38161 taggtctatt atagtagact ctgtagctcc tttaggaatt gcctttttta tatgtgcatc
    38221 aaagattgat aagtacaaga tacctgtatt catatcaaca ttattaggaa tattgttatc
    38281 atctaatgat tttggaaatg tagcaaaata tgctgtgtgc cttacaatat ttatggctat
    38341 gaataaaaaa ttaaaattat cagattcaat tgctaaggtt gcatttatag gtgcaataat
    38401 actacttcca atctccatgg gacaagcttt cttttcaaat aggtatgtat atggtcttct
    38461 tatagcagga gtagaagcaa taataatgtt tatatctata tatgtgtttt cttatgggat
    38521 aggtttaatg gccaatgtca acaataggat gtctgtacgg acagaagaag ccatatcaat
    38581 aagtttactg ctaaccttta gcataatggg aataggagat atatctctgt ttggaatatc
    38641 aataaggacc attttatcga ccgtattgat attgctgacc tcaatagtag gtggagctac
    38701 tatgggagca tctagtggtg taattattgg tgtagcatcc attttaaata atatgacatc
    38761 agctgtttat atggggatat attctttttc aggattaata agtggagcat ttaataaaat
    38821 aaataaatat ttctgtatac taggatatat tctaagttgg acaataatat acctatatac
    38881 ttctggtatt acttctaata tgatgcaatt aagagatata ctattaggtt gtttgatagt
    38941 actagtattg ccagagcgat tatttaataa gattgaaaaa ttaataaaaa gtaatgtggc
    39001 ttcaaatgaa atcgtatacg attatattat gagaagcaaa aacctaacta atagcagatt
    39061 gaacagtatt tataaaactt atgatgacct tgcagataca tttgataaga taagagaaaa
    39121 agacaaagtg ctagaccaaa gagacatagc taatgtaata gatatgattc ataatgatga
    39181 atgtaaaagt tgtagtatga gaagaatgtg ctgggagtca agatttaatc atacatacac
    39241 tatgatatat aacattcttg aaaaaattga ggagaaaggg gagttaagtc taaacgatat
    39301 acctaaaaat tttagaaagg aatgtatgaa accagagtca atagtgaaaa tatcaaatca
    39361 ctattacaaa atgtttgtat tagattatga ttggagtgtg aagttttcag aaagtagaaa
    39421 gttaatagca aatcagataa gaagtatttc aaaatccatt aaatccttat ctcaagattt
    39481 agaaggcgac ataatgctag atatagaaaa ggaaaaaaat atatatgaac agttagaaag
    39541 atatgatata acagttgata aagtaagtta cttaacaaaa agtaacagtg agtttgaaat
    39601 atcaatagaa aagaaaactt gccatgatgg atgtatgtgt gaagataaaa ttgtaaatat
    39661 aatttcagac ctagtagggg aaaatatgtc tgttagaaaa ataggatgtc actgtttagg
    39721 aggtaaatgc aaggcaacat tcgttaaatc tcaaaaatat aaggctgtta cagaagtttc
    39781 agcaatgtca agggatgggc atatcctttg tggagacaat tacacataca tggagataaa
    39841 tgatggtaaa tacatgatgg ctataagtga tggtatggga aaaggtaaaa aagcatatga
    39901 agaatcttct gccactattg atatactgga aaaaatgata gatgcaaaaa ttaaagatga
    39961 gatagtcatt gatactataa ataatatgtt gttgttaaag tcttcagaag agatgttttc
    40021 aactctggat ttgggaattt tagatttaaa acgaggttgt ttagaaacta taaagatggg
    40081 agcgtgttct acttatataa aaagagagga tggagaagtt gacttaatat cgtcatcatc
    40141 attacctgtg ggtatattat cagatgtaaa aattgataga aaaaatgtaa aagttaagga
    40201 aggagactat gttataatgg tttctgatgg aattgtagat gcaggaagaa ataacaattt
    40261 aggagacaat tggttaatat acttcttaaa gaatatagag actacaaatc caaaggaaat
    40321 ttcaaattta atattggata gagcattaga gttacaagcg ttacaaattg aagatgacat
    40381 gactgtttta gttacgaaaa tatgtacaaa ataaggttta caagattatg tgtataagat
    40441 gtggataact tgtgtataat atgttaatag acttttgaaa aaagtatagt tattaacaaa
    40501 aataacatat aaatcaacaa aatttgttga taagtatggc agaaatgtta ataaaatgta
    40561 aaaaaagaaa aaacaaaagc tattatcaaa agagattcat gttgaaataa ctaaatttct
    40621 aagtaaagaa aacctgtgaa tatgttgatt attatgtgga taactattaa ttcgatattt
    40681 atttatatag tatgaataat tgaataatat atctaaaaaa agccatccta ttattaagta
    40741 aacttattga aaggatggct ttttatgaat agaaaattgt gcaaagcaat aataccttta
    40801 tttatttcta ttttttgcat gttaataata agtataaaag ctgaccaagg atatcaaaag
    40861 aataatgata gagcaattac tgtcatgaat aataatgata ctgaagatga tgctgaaaaa
    40921 aaacaagaaa tagactatga aaaaacatgt tggaatttgc aatctttgtt taaaagtgat
    40981 gaccaatgga aaaaagaact taagagtttt gataaagata ctaaagagtt gaaaaattac
    41041 ataggtaaag taacaaaatc gccaacacat ttgtcttttg ccttaaacat taaagaaaaa
    41101 cttgatataa gactaaataa actttctgcc tatgtaaaat taaaacaaga tactaataaa
    41161 aattcgtata agtacttaga tatgaatgat agtatgagta aatcctatgg gaattatatg
    41221 gggatttgtt cagatttaga gttagaaata ctaaaattat caaacaaaga ttacaagaaa
    41281 attatttcaa ataaaaatat aaataggaaa tatggtgcat atttaagaga tataagaagg
    41341 aataaagtac attatttgga tgataaatca gaagatattc taagcaaagt atcttcaata
    41401 agtagtcttc caagccaagt atatgaatta tttagaaata tggatagaaa aactaatttg
    41461 acaccagctc aatatgcgag tgaattagag tcatcagata gagaaaatag aaaaaaagct
    41521 tatcaagatg aacttataac ttacaatgat aatataaata caattgcagg tttaattact
    41581 ggtcaagtta aaaagaatat tttttattct caggaaagag gttatgagtc ttctttagaa
    41641 atgtacctgg aatctgataa tgtagatgaa aaagtataca ataatttgat aaggactgta
    41701 aatagtaata tggacagtct acacaaatat atagatttaa gaaaaaaagt attaaaatta
    41761 gataaggttc attcatatga tatgtttgtg cctatggtta aggctgttga taataatatt
    41821 aattatgaaa aggcacaaag tatagtatat tcagcgttgt cacctttagg aaaagaatac
    41881 aatgatgtag tatataaagc tttcaatgaa aaatggatag atgtttattc acatgaaaat
    41941 aaagtaagtg gtggatactg cttatctgtt tatgaaaatc atccatatat acttttaaat
    42001 tataataatt cattaggttc agtttcgact ttatcacatg agcttggaca tgctgtatat
    42061 gaatatttaa gttctaagaa tcagaattat tttaattcaa atccgtctat atttactcac
    42121 gaagttgcat ctacaacgaa tgaagcatta ctttacgaga tgcttataaa agaagctaaa
    42181 ggcaatgatg aaaaagctta ttatataaca cagtatttag acttgataaa agatactctt
    42241 tatacacaaa ctatgtatgc tgaatttgaa aaaactatac acgagttagt agaaaaagga
    42301 aaaagtgtaa acgcattagt attaaatgat gtttggggac aacttcttaa aaaatattat
    42361 ggacaaagct atgaattaga ccaattatct aaggtcggat gggctagaat cccacatttt
    42421 tataatagtt tctatgtcta taaatatgct acaggttgta gtgcaggagt aagttttgcc
    42481 caagacatcc taaagaatgg ttcagataac tatatgagct ttcttaaaaa gggaagttca
    42541 gattatccaa taaacttgtt aaaatcaagt ggcataaatt taactaatac aaaacccata
    42601 gaaaatacaa tcaaaaaatt tgatagttta gtagcagaac ttgaaaagct attataaaca
    42661 ttattgtaat tcacatggta aatatatcga attaatgata aaatatacta aaagtttaaa
    42721 gtcctatcaa gataggactt tttatttatt aattagtggg agatgattct atgcaagtta
    42781 aagttaaaaa ggctgtaata ccagcagcag gtttaggaac tagatttttg ccagctacaa
    42841 aggcacaacc aaaagagatg ttaccaatag ttgataagcc aactctacaa tacataatag
    42901 aagaagctgt ggcctcagga atagaggaaa tactaataat aacaggtaga aataaaaaat
    42961 ctattgaaga ccactttgat aaatctgtag aattggaact tgatttagag aaaaaaggta
    43021 agaaagaatt gttggaggta gtacaaaaca tatctaacat gattaacata cattatataa
    43081 gacaaaaaga gccaaaaggg ttgggagatg caatttattg tgcgaggcat tttataggtg
    43141 atgagccgtt tgcagtaatg ttaggggatg atattgtaga taatgatgta ccttgtctaa
    43201 aacaactaac agatgcatat gaggaatata gaactacaat attgggtgtt caaaaggtaa
    43261 atcaagaaga tactaataaa tatgggataa tagaagcaaa aaatatagaa ggtagagttt
    43321 acaaggtaaa agacatggta gaaaagccag agtcaggaaa agctccttca aatattgcta
    43381 ttttaggtag atatataata actcctgaaa tctttgatat tcttaaggat ttaccaccag
    43441 gtaaaggtgg agaggtacaa cttacagatg ctttaaaaat attatctaag aaagaagcta
    43501 tgtatgcata caattttgaa ggcaagagat atgatgttgg ggacaagcta ggatttttag
    43561 aagctactgt tgactttgct ctaaagaaag aagaccttaa ggaagatttt ataaaatatt
    43621 tgaaacatgt atgtagtgaa tttgataaaa ataacaatgt gttatacaat aatacagatg
    43681 aaaaaataga tttaattgaa gtcaatgaag ctagagaact tgaaattcaa aaataattaa
    43741 tgaaaggtga tgtatggtgg caaaatcaag tcagaaaaga attaatagaa taatagctgt
    43801 tgggattgtg ggaatcctcg taataacgag tataacagct gttttgagta tgattgcaac
    43861 tatgtaataa gataaatgac agcagttgat atacataaat ataaaaacca tatataactt
    43921 gatgtagtca aaaacttaca atattttagt tatatatggt ttttagaatt tataaatgta
    43981 aaaaaaaatt tttataatag ttgcatattt taaaggaaaa tattattata agatataaat
    44041 atagtgataa atttataaaa acgtgctaag attggacttg aaatacagta atatagtagg
    44101 taataatgtt ttaaatttta tttattgacc ataatatata aataatgata ttattagact
    44161 taactgttta aataaaactt ttctttttca tgccttcaat ttccaaaata agtatgtata
    44221 taaatgaata tatagatgag gaggattttt ttgattttag atgataaagt attagaagga
    44281 ttaggtagta agaccttagt tgagctacga gagatagcta aagaattaaa aataaaatca
    44341 attactacat ataagaaaaa tgagttaatt gagattataa attccaaaag taaaaatgag
    44401 gaaaaagtaa acgaagagac taaagaaaaa aatgaagata ttaaagaaga cataaagaat
    44461 agaaaagaaa atatagagcg tgaaagtata gaaaaagaag ttgaatataa gagccctacc
    44521 aaatcataca acaagaaaga aatagatacc caaaatattt cccaaaacca agaacataga
    44581 aatcaaagaa atgactataa taagacaacg agtgtaaatg atagtaataa gtcatcaaat
    44641 atatctaata gaatggtcag aaataataat aaaaactatt atatgcctaa acaagttgat
    44701 gaatctaaga tagtagatga atttaacaca tcaaaagagg atgaagtagt aggtgtactt
    44761 gaaattctgc cagatggatt tggtttttta agaggttcaa attatttgtc tactgaagga
    44821 gatgtttacg tttcaccatc tcaaataaga agatttaata tgaaaacagg agacaagatt
    44881 aagggaataa ctagacatcc aaaaagtgga gagaaattta gagcactttt atatgttcaa
    44941 aaaataaatg atgaaaaccc tgacacagct atacaaagaa atgcgtttga gacattaaca
    45001 ccaatatttc cagaagaacg acttacactg gaaactaata gaaatgaaat agctacaaga
    45061 attatagatt taatttcacc tataggaaaa ggacaaagag gattgatagt agcacctcca
    45121 aaagctggga aaacagttct tttaaaaagc gttgcaaata gcatagctaa aaatcatcca
    45181 aatgttgaac ttatagtact ccttatagat gaaagaccag aggaagttac tgatatgaaa
    45241 gaatctattg aaggagacgt tatatattcc acttttgacc aagtatcaag tcatcatgtc
    45301 aaggttgcag agatggtgtt aaatagagct caaagattag ttgaacatgg taaagatgta
    45361 gtaattttac tagatagtat aaccagattg gcaagggcat ataaccttac aatatctcct
    45421 acaggtagaa ctttatctgg aggtattgac cctggtgcac ttcatggacc taaaaagttc
    45481 tttggtgcag caaggaatat tagacagggt ggttctttga caatattagg aacagcattg
    45541 gttgaaactg gttctaggat ggatgatgta atatttgagg aatttaaagg tacaggaaat
    45601 atggagctac atttagatag aaaattagca gaaaaaagaa tattccctgc aatagatatt
    45661 tacaagtctg gtacaagaag agatgattta ttgcttgatg atgaagaaaa gactgctctt
    45721 tggagattaa gaagagaaat gagtaataat tcagtaatgg aaattacaga taaagtcatt
    45781 gaacttataa agagaactaa ggacaataaa gagtttgtaa aatcaataaa gaatttgtaa
    45841 aatcaataat acaaatgcaa atatggatta tataaactaa tagaaaaatc acttgaatgg
    45901 cataataagt tgtgctataa taatgtagtt gataatgtaa aaagaaagtt aatataatta
    45961 atttttttaa ataaagagaa gaggtgacta taatgcaaaa ggaaatacaa ccaaaataca
    46021 atccagttga agtgcgttgt gcttgtggga acacatttgt tgctggttca actaaggacg
    46081 aaataaaagt tgaaatatgt tcagagtgcc atccattcta tacaggaaaa caaaagaaca
    46141 tagaaaaagg tggaagaatc gacaaattca agaaaagatt caaaatggac taatttttat
    46201 aaaagtggag ttggaggaag ccaactccta ttttattaca aaaaaacatt catattctac
    46261 tttaaattgt ctttttgtat tataataata aatatgtaaa attgagtatt ctaaatacaa
    46321 taaaatgagg tgagtggatg gcaaaacaag ctgtaggtgg gcaagctgtt attgaaggtg
    46381 taatgatgca gtcaaaagac aaaagagcgg tagctgttag aaaaagtgac ggagaaatag
    46441 cactaaaaga agatagaata aaaagttggg taagagataa gaatatagat aagatacctt
    46501 ttgttagagg ttcttttgtt atgatagata caatgattca aggcataaaa agtttaaact
    46561 tttcttcaga gttttttatg gaagaagctg aagaggataa atttgattta tttataaaga
    46621 aaatttttaa agataaggca aatgatataa ttataatatt ttcattggtc atagcaatgt
    46681 tactttctgc tggattattc atttttatac ctacattggt aggaggagct ttttctaaag
    46741 taatgcctaa tgattttatg cttaatttaa tagaaggaat cataaggata gctatattat
    46801 ttgcatatat agtattgatt tcacgaagta aggatataga aagagtcttt caatatcatg
    46861 gagcagaaca taaatctata tattgctatg aaaatgattt agaacttaca gtagagaatg
    46921 ctagaaaatt taaaagatta catcctagat gtggtacaaa tttcctattt atagttatgg
    46981 ctgtatcaat aatattgttt gcattttttg gttggccaaa tccaattctt agaatattta
    47041 tgagaattat atgtgtgcct atagtagcag gtttatctta tgaggtaatt agagttcttg
    47101 ggaaatatga taatggattt actaaaatta ttgcatatcc aggaatgatg ttgcaatatt
    47161 ttacaacaaa agagcctgat gatgagcaat tagaggttgc tttggaagca ttgaaagcag
    47221 tagtagatta gagaaaagga tatttgttat gacgataaaa gatataatta ttaaatattc
    47281 agataaacta aaagatataa gtgatactcc aagattagat acagagttat tgttacaaaa
    47341 aacattgggt gttgatagat tatatattca cttaaatctg aataaagagc taactgaaga
    47401 acaaaaaaca aaatttatgg gttttgcaga agaaagatta aatggaagac caatagcata
    47461 tatagttgaa aatagggagt ttatgggatt agattttttt gttaaggaag gcgtattgat
    47521 tccaagacca gatacagaaa ctttagtaga agaaataatt gaaatatgta gagaaaaaaa
    47581 agatgtaagt atattagata taggaactgg ttctggagct attacaataa gtttagcaaa
    47641 gtacattgaa aattctaaaa tcatgtcttt tgatatatca gagacagcat tagaaatagc
    47701 taaaaaaaat gctattataa atgaagttgg tgaaaaaata aagtatatta attctgattt
    47761 atttacagca ataagtgata gtaacataaa attcgatatc atagtttcaa atccaccata
    47821 tattaaaaaa caagatatag aaactctgca caaacaagtt aaagattatg aaccatataa
    47881 tgctcttgaa ggtggagaag atggattaga cttttataga agaataacag aacaaggtaa
    47941 gaaatattta aataagtgtg gaatattagc atatgaagtt ggtcataatc aagctgaaga
    48001 tgtaataaat attatgaaaa gtaatggata taaaaaaata tacacaaaga aggatattca
    48061 aggcattgat agagttgtta taggttacaa tatatgatat aatcaacaat tgatatgtta
    48121 catttaatcg aggtgaaagt atgttaaaaa aattagaggt attggaagat acatataaag
    48181 acttaagtga aaaaataggt gatccggatg taataaatga tcaaaaagtt tggcagaaat
    48241 atataaaaga acatgctgac ttggaaccta ttgttatgaa atacagagag tataaaagcg
    48301 ttttagatag tataaaagaa tcaaaagaaa ttttacaaga agagtcagat gaagaattaa
    48361 gagaattagc taaaatggag ttagctgaga tggaagaaaa agtagcacct ctggaggaag
    48421 aaataaaaat actattattg cctaaagacc ctaatgatga taagaacgtt atagttgaaa
    48481 ttagaggtgg agctggagga gatgaagcag ctctatttgc tggtgattta tttagaatgt
    48541 actcaaggta tgctgaaaga agaagatgga aaatagaatt attgagtgca agtgatactg
    48601 gagttggtgg atataaagaa gtatctttta tgataaaagg taaaggtgct tattcaagat
    48661 taaagtatga atctggagtt catagagttc aaagaatacc atcaactgaa tctggtggaa
    48721 gaatacacac atcaacttct actgttgcag tattaccaga agttgaagat gtagaggttg
    48781 aaataaatcc aaatgattta agaatagatg ttttccgttc ttcaggtaat ggaggacaaa
    48841 gtgtcaatac tactgactct gctgttaggg taacacatat acctacaggt gaagtagtat
    48901 cttgtcaaga tggaaaatca caattaaaaa ataaagagca agctttaaaa atattaaaag
    48961 ctagacttta cgataaagca ttggcagaac aacacaaaga tatagcagca gaaagaaaaa
    49021 gccaagtcgg aacaggagac aggtcagaaa gaataagaac atataatttc cctcaaggta
    49081 gaataagtga ccatagaata aatttaactt tatataagtt agatgcattc ttagatggag
    49141 atatagatga aatgatagat gctttaatta ctgttgacca aacagaaaaa atgactgcaa
    49201 tataagtaat gttttaaaga agctgtttct tcctagaaat tattttctat tttgaaacag
    49261 ctattttgca ttttaggtca tgttatcata ttctattgta tataattgtt atatacaagt
    49321 ataggagggt tatatgatac taaaagtaac aataattggg cttttggcag gtgttatagg
    49381 gacaggatta ggaggagtaa tatcagccat ttttaaaaga gaggtcgata aatatctaag
    49441 tttttttatg ggattatctg gtggaataat gttggctgta gtggtgtttg atttaatgaa
    49501 agaatccatg gacaaaatgg ggataatcaa tacagttata tttacgtttg ttggagctct
    49561 cataactatg tacataaaaa caaaattaga tgtgtctgga aatatggcat ctggatatct
    49621 tatattcata agtatattac tacataattt accagaagga ctagccattg gttcttcttt
    49681 tatgtcaact gaaagtttag gtattacact tgctatagtt ataggtctac ataatatacc
    49741 agaagggtta gctatggcat taggcttagt ttgcaataaa atgaaactaa gtaaagttat
    49801 tttgtttact gtaatagctg gattacctat gggtctagga agttttttag gtgtttattt
    49861 tggaggagta tttacttctc ttataggagt atttcttgct acagcaggag gtactatgat
    49921 gtatgttgta ttggaagaaa tttttccaca ttcaaaaagt gtgtattgta taattggttt
    49981 cttgttaggt acaatgattg taaattatat ataaaattaa aatgaaagca ggcatacaaa
    50041 agatgaaaac tattataagt aatattgata taaataatat tgattatgag gaaataaaaa
    50101 tacaagcaaa acttcttaga gaaggtaaaa cggttatttt tccaactgag acagtatatg
    50161 ggttgggtgc aaatgcatta gatgaaaatg cagtaaagaa gatttatgaa gctaaaggaa
    50221 gacctagtga taatccattg atagtccata tatatgaaaa agaagaagta tatgacttag
    50281 ccaaagatat aagtgataaa gctaaacttg ttatagaaaa gctttggcca ggacctataa
    50341 caattatatt aaataaaaaa gatataattc catacaaaac tagtggaggg cttgatacag
    50401 ttgcaataag gatgccttct aatgttattg ccagggcaat aataaaagaa gctggtatac
    50461 ctattgcagc tccttctgca aatatctcag gtcgcccatc tccaacaaaa gcaaaacatg
    50521 tatatgaaga gatgaatgga agagtggatg gaatagtttt aggaggagac agtaattttg
    50581 gattggagtc aactgtattg gatttaacag aagagactcc tatgatactg agaccaggaa
    50641 gtataactaa agaagtttta gaggaacttc ttggagaagt aaagcttgac ccatcattaa
    50701 gtaaaaaaga agataatcaa aaagctaaag ctcctggaat gaaatataaa cattattctc
    50761 caaatgcaga tgtatatata atatctggaa aaagagaaaa tgtagcaaca aagattaatg
    50821 atatgataaa atctaataga gacaaaggtc taaagacagg tgtaatgtgt attagtagaa
    50881 accgagagtt ttatgatgga gaagtaatcg atttaggaaa ctctcttgaa gaagttgcaa
    50941 gtaatctgtt tgatgctctt atagaaatgg acaaaaaggg tgttgatgtc atatattcag
    51001 aagagttccc caagactgga gttggacaag caattatgaa tagactactg aagtcagcag
    51061 gatacaaagt gataaaggct taagagggag ttgtaaaata tgaatatact tattgtgtgt
    51121 acaggtaata cttgtagaag ccctatggct gaggctatac taaggaaagc aattaaagaa
    51181 tctggaagaa gtattgaaga atattctata tcatcagcag gtatatctac agcaaatgga
    51241 atgggtgctt ctgaaaattc tatagaagta ctaaaagaaa tcggtataga cttatcaaat
    51301 catagaagta aagtaattac taaaaaatta attgatgaat cagatataat actaacaatg
    51361 actaaatctc ataaagaaat tttagtacaa gcagttccaa aatgcaaaga aaaagtgtat
    51421 acttttaaag ggtttgcaaa taaaaatgag gaagatatat ctgacccatt tggtggaaat
    51481 ttggatattt atagaagcac tatgagagaa ataatgtatt ctgtcaacga aatagtaaaa
    51541 aaaatatgaa acggaggata aacaaatgaa gataggatta ggctgtgacc atggaggata
    51601 taatttaaaa aaagaaataa taagctattt agaaagtaaa ggtatagagt gtgtagatta
    51661 tggaactaat aatgctacag attctgttga ttatcctgtt tatggagaaa tagttgcaaa
    51721 ttcagttata aataaagaag tagattatgg aatattatgt tgtggtacag ggataggaat
    51781 atctttagct gctaataagg ttcctgggat aagatgtgca gttgtatcag atgtattttc
    51841 tgcaaaaatg tcaaaagcac ataatgatgc aaatatgtta tcattaggcg aaagagtttt
    51901 aggaaaaggt ctagcacttg agatagttga agcatggatt aatacggatt ttgaaggtga
    51961 tagacatgct agaagagtaa acatgattaa atcaatagaa gaaaaacata ataaataata
    52021 aaaattacag ttttatcagg aggaattatc aaaatgagca aagtggtaga gacaaaccat
    52081 ccattaatac aacataaatt aactttaatg agagataaaa atactggttc taaagacttt
    52141 agagagcttt taactgaaat tgcaatgtta atggggtatg aaataacaaa agatatacca
    52201 ttaaaagatg tagaaataga aactcctatt caaaaaacat catctaaagt agttgcaggt
    52261 aaaaaattag caataattcc aattttaaga gcaggtcttg gaatggtaga tggattagtg
    52321 agcttaatgc cagcagcaaa agttggacat gtaggactat atagagaccc agaaacatta
    52381 aaaccagttg aatattattg taagcttcct caagacatag gagaaagaga tataatagta
    52441 gttgacccta tgcttgcaac agggggttct gctgtagcag ctatagattt acttaagtct
    52501 aaaggagcta aaagtataaa attagctaat ttagtagctg caccagaagg tatagcagaa
    52561 gtgcaaaaat atcatgatga tgttgatata tatgttgcat cagttgacga aagattaaat
    52621 gaacatggat atataattcc tggtcttggt gatgctggag atagattgtt tggaactaaa
    52681 taaaaataaa taactataaa tatttattag ggactttctt atatagtgta tataatttta
    52741 tgtaagaagg ttctttttta tatatttttt ctcaaaatag gaattattga attgataaat
    52801 ttctcgaatt gagaataaaa agtttttacc aattaaaaaa gattgcttat tttacaaggt
    52861 tttatagaat agtagtctat tttaatgtgg catgttagtt gcttacgatt ataggtatta
    52921 agtgtattag ttaaattgag aattgaatat taataatatt attgggagtg gtaatacatg
    52981 aagaattgta tagtagtaag aggggctgga gatttagcaa cagcagttat tcataaatta
    53041 cacaacagtg gttttaaagt agttgcgtta gaaatagaag agccattagc tataagaagg
    53101 caaatttctt tttgtgaggc tgtttatgaa aaaaaagtaa ctgttgaagg tgtagaatgt
    53161 atattttgca atactgaaaa agaaatagag gaagttttat ctcaacataa agtagccttg
    53221 attatagacc caaaaggaga aaaattatct aaattaaatc caatcgtttt ggttgatgga
    53281 atactcgcta agaaaaactt aggtacaact aaagatatgg caccacttac aatagcttta
    53341 ggtcctggat tttgtgcagg agaagatgta gatgttgtta tagagacaat gagaggtcat
    53401 aatctaggaa gaataataac taatggtaga gcagaaaaaa atacaggagt accaggagca
    53461 ataaaaggtt tttctaaaga gagagttatg cattcacagt atgatggcat aatggaaaat
    53521 gtatgtaaga taggtgatat agttgaaaag ggagacctac tttgctatat taataattat
    53581 gaaaaaaaac atgaagttag agcaacaata tctggagtcc ttagaggttt attaaaggat
    53641 ggagcaaagg taaacaaaaa tttaaaaata ctagacatag accctagaaa agaagaagtt
    53701 aagaatgctt ttacaatatc agataaagct agatgtatag ctggaggggt tttagaagcc
    53761 atactaaaca atgggattta tccccaaaat taatttatat tagataaagc tattaatatt
    53821 ttattaagat gatataataa atttaataga ttaaagagag catatgacgg gtatttaaaa
    53881 tttaaatggt aaaaatgctc tttttttatt ggacattcaa ttaacatgtg tatataatat
    53941 aaaaataagt atttatttag gttgaaatct aataaatata taataccagt acataagcaa
    54001 agaggagaaa agtaaattat gagaccatca tgggatgaat acttcatgga aattgcagaa
    54061 gtagtgaaaa agcgttcaac ttgtattaga agacaagtag gagctgtaat tgtaagagac
    54121 aaacaaatat taacaactgg atataatggt tctccgagaa atcttgaaca ctgtgaaaat
    54181 attggatgta agaggcagga attaaatatt ccctcaggag aaaggcatga attatgtaga
    54241 gcattacatg ctgaacaaaa tgcgataata caagctgctc ataatggtat tagtgtagat
    54301 ggggcaactt tgtatgttac tacaagacct tgtgttttgt gtgcaaaaat gtgcataaat
    54361 gcaggtatat tgaaaattgt ttatgaaggt gattatccag atgatatgtc tacagaatta
    54421 ttaaaagaag caggaataga actcataaaa ttttagtgga taaaatttat atgacaataa
    54481 atgtcaaatt atgacactga aatacaatgg tatcaaatga ttaaaagaaa gtatttgaaa
    54541 aatcatcaga ttagtagtaa gatatatttt ataattaaat aaatttatga gatagattaa
    54601 ttacatgtta tttattttat aataatgagg gatactattt atgagtaaaa gaaatacaca
    54661 tacagaaatt gcaaagttgt ttagtttaat aagtcaaata ggattgatga ttgtaatatc
    54721 aatattagga tgtactttct taggaaaatt tttagatagt aaattgaata ctactccagt
    54781 tctgacaata atatttttat tgttgggtgt tggaggtgct tttagtggtg tttataaaac
    54841 tctaatagta tatacacgaa ggaagtgata tattggatat aaaattactt aaccaggtaa
    54901 ggtcagtaac aaaaggtata gtaatatttg atgtagtttt aattgtgcta atgttgataa
    54961 catcaacatt aagtaaacca gtattattag gacttatatt tggctctata atagctatgt
    55021 taaattttag attattggcg atatcactgg aaaaatcagt gacatttcct gctgggaagg
    55081 cacaagctta tgcatcagca caatatgctg ttagattatt tataatggca gttgttctaa
    55141 ttgcatcagc tacagtacca catttaaata taatcggaac agcattggga ttattgagca
    55201 caaagtttgt catattatca aaaaacttta taaataaatt aaagaggaag gaggcataag
    55261 tatgaatcaa gcgcattatt atctcatagg aaactttaag attagtgaaa caatactagt
    55321 aagttgggga ataatggcct ttttagcgat agcatcttat tttttgacta gaaacttaaa
    55381 aaaagttcca actagtaaag ttcaaatatt cttagaattt gcagtaggtg gtcttgctaa
    55441 aatggttaca gatgttatgg gtaaaaatac agtaaaaaga atgccatata tgataccata
    55501 cataggaagc ttgtttttat ttttcgcatg ttcaaattta agcccattac ttggattaag
    55561 gtcaccaaca accgacttag acacaactgc tgcatgggca atgattacat tttttatgat
    55621 ttattttgca ggagttaaat ttaaggggtt atcatacttt aaagaattgg ctcaaccact
    55681 tgccataatg ttaccaatca atattatagg tgagttagct agaccaatat cattaagttt
    55741 ccgtccattt gggaatattt taggtggaac aataataatg ggtctgtttt atcaactttt
    55801 aggttttata tctagtttaa taccaggatt atcaatacca attggagaaa tattaatacc
    55861 agtcccatta cacttgtact ttgacttatt tgcaggattg ttacaagcat atatatttat
    55921 aatgttaaca atggtattta tatcaagtgc aggagagtta gattaagaaa tcaacttata
    55981 ttaagataaa aataaaataa tttaaaaaat taaagtttta aggaggaaat aattatggaa
    56041 acagcaatag tagcggcagc atcagcaata ggagcaggga tagcagttgc aacaggtata
    56101 ggtgcaggta taggtcaagg tatagcagca gctaaagctg cagaagcagt aggaaaccaa
    56161 ccagaagcta aaggtgatat aacatcaact ttattactag gagttgctat agcagagtct
    56221 tcagcaatat atggtttggt tatatcaata atacttctat tcgtaaatcc attctttaaa
    56281 tacttaggaa tgtagattta gtccttaaat attttgagat aggtttatag gtaaatcatc
    56341 tcaaacttat aatcatgaga tgaactttat catctcgtga ttataagtag ttatgattta
    56401 tggaaaggag gataattatg gaaaaagcac tagtaggtat aacgtgggaa tttgtgttcc
    56461 aaattgttaa tacttttata atatttttac ttctgagaaa gcttttattc aagccagtat
    56521 taaatataat tgaatcaaga gaaaatgata ttaaaagtga tttggcagaa ggcgaaaaag
    56581 caaaaaatga aggtttagct ctgaaaaaag aatatgagtc aaaaatcaat tttgccaagg
    56641 atgaaggtca agaaatcata aaacaagcaa ctataagagc agagcagaaa tcagatgata
    56701 tagtaaatac tgcaaagaaa gatgctcttg atataaaaga aaaagcaaac aaagatatag
    56761 aacaagaaag acaaaaggtt ataaatgaaa tcaaaaatga tatatctaac atagcactac
    56821 ttgcagcatc taaagtcatt gaaaaagatt tagataaatc taaacatgaa gaattgatag
    56881 aaaactttat aaaagaggta ggggaagcta aatgataaat gtaatagcaa atagatatgc
    56941 agaagcccta tttcaattag gagaagaaga aaattcaact gatgtcttat tcaaagaact
    57001 agaaaaagta gtagatatga tgactaaagt tagtaaagat ttctacaaag ttttaaaatc
    57061 accattagtt tcaaaatcag aaaagaaaaa cttggtagaa attattttta gtaaagaagt
    57121 aagttctaac ataaaaaact ttttaaaagt tttagtagat aaagatagaa tatcatattt
    57181 agaagatatc gaattagctt ataaagaatt attaaataag aaaaacaatg taattgatgg
    57241 agtagcaata agtgctattc caatgagtga aacagatatt aaagaacttg aagtcaaact
    57301 ttcaaataaa tacaacaaaa atgttactat agaaaatgtt gttgacaaga ctatactagg
    57361 gggagttcta gttagaattg gaaatgaaca aatagatggc actgtgaaaa ctcgtcttga
    57421 taagatgaaa gaaaagctat ctgaagtaat atcttaagaa tggcggtgaa cccatgaact
    57481 taaaacctga agaaataagt tctataatta aacagcaaat aaaaaattat gagaataaag
    57541 ttgagttgac agatacaggt agtgttttaa cagttgggga tggtatagcg agtgtatatg
    57601 gattagaaaa agctatgtct ggtgagttgc tagagttccc aggtgaaata tatggtatgg
    57661 cacttaactt ggaagaagaa gtagttggag ctgttatcct tggagatgac agtgaaataa
    57721 aagaaggcga tatagttaag agaactggaa gaatagttga agttccagtt ggagaagctt
    57781 taataggtag agttgtaaac tcactaggtc agccaataga tggaaaagga cctatagcgt
    57841 acacaaaaac tagaccagtt gaatctgagg cacctggtat aatagataga aggtctgttt
    57901 atgaaccatt acaaacagga atcaaatcaa tagattcaat gataccaatt ggtagaggtc
    57961 aaagagagtt aataataggt gacagacaaa ctggtaaaac atctatagtt attgacacta
    58021 tactaaatca aaaaggaaaa gatgttatct gtatatatgt agcaattgga caaaaacgtt
    58081 ctactatagc tcaattagtt agctctttag aaaaaggtgg agctttggat tatacaatag
    58141 tagtttcagc aacagcatct gaatcagcac cacttcaata tatagcacca tatgctggag
    58201 cagctatggg tgaagaattt atgtacaatg ggaaacatgt acttatagtg tatgatgatt
    58261 taagtaaaca agctgttgca tatagagaaa tgtcattgtt acttagaaga ccaccaggac
    58321 gtgaggcata tcctggagat gtattctatt tacattcaag attacttgaa agagcagcta
    58381 agttatctga tgaattgggt ggaggttcaa tgactgcctt gcctataata gaaactcaag
    58441 ctggtgacgt ttctgcatat ataccaacaa atgttatatc tataacagat ggtcaaatat
    58501 acttacaacc agagttattc tattcaggtg taagaccagc agttgaccct ggtatatcag
    58561 tatcaagggt tggtggttct gcgcaaatta aagcaatgaa aaaagttgca ggaacattaa
    58621 aacttgcata ttcacaatat agagaacttg cagcattctc acagtttggt tctgacttag
    58681 atgaagatac taaaaagaga cttgctcaag gtgaaagaat cgttgagata ttaaaacaag
    58741 gtgagcatca accaataaaa gttgaaaatc aagttatgat aatatatgct gttataaata
    58801 atcatttaga ggatattccg atagataata tagctagatt tgaatcagaa ttgtatgcat
    58861 ttgtagataa taattatccg gaaatatcaa gaaaaatatt aggtggagaa gacttcactc
    58921 atgatttaac agatgcaata aatgaattta aagaaaagtt tgttgttgaa gtataatcct
    58981 ttttaaaaaa ggaggtaaat taattggcag gagctggaat caaagcaatc aaaacacgta
    59041 tgggaactgt tgaaagtaca aagcaaataa ctaaagcaat ggaattagtt gcatcttcta
    59101 agcttagaaa agcaaaagaa aaggctgaag attcaagaga atattttaat actttataca
    59161 atacagtaaa agatattgcc caaacaacac gtggaattag aaatgaattt ttaaaagaaa
    59221 gagaagtaaa aaataaatgc tatgtagtag tagctggaga cagaggactt gcaggtggat
    59281 acaattctaa tgtattaaag gaatctgtct ctcatatgaa aggtaaaaag gaaacagtga
    59341 ttactgttgg aaagaaagca catgaatttt tttctaaaag aaactacaat atagcaaaag
    59401 atataaatag tgttgaagct tgtggatatg aagatgcgac agaaatttca caaactatta
    59461 ttgacctgta taaaaaaggt gaagtagatg aagtttatat tgcttataca aaatttaaat
    59521 ctgctttatc acaagaagtt gaaattttga aattgcttcc attagtcttt gaaaaagata
    59581 cagacgaaca aggaatggca gtaaaagaag aaaaacaaaa tagatcaaaa gtacaatata
    59641 taccatcagc agaagcagtt ttaagttata ttgtacctca gtttatatca ggtacagtat
    59701 atggtggaat tttggaatct tttgcatcag agcaaggtgc tagaagaact gcaatggaat
    59761 cagccacaga caatgcgaat gaaatgctat ctaatttaga gttaagttat aacagagcaa
    59821 gacagtcagc tgtaactcaa gaaataacag agatagttgg tggagttgaa gctctaaaat
    59881 agggaggtta ggaaatggca aatgtaggta aagtagtaca gattgtagga gctgtactag
    59941 atgttaagtt tgatagtgaa caaagcctac ctaacttatt aaatgcgtta gtaataaagt
    60001 taggagataa agaaatagtt gcagaagttg ctcaacatat aggtgatgat actgtaagat
    60061 gtatagctat gagtgcaaca gacggacttg taagaggtat ggaagttgta gatacaggag
    60121 ggccaataag tgttcctgtt ggagatgaaa ctttaggtag aatattcaat gttcttggaa
    60181 aaccagttga tgggaaacca gctccaaaat ctgcacctaa attacctata catagaccag
    60241 caccagcata tgatgaacta gaaactacag cagagatact agaaactggt attaaggtag
    60301 ttgacttact agctccatat ctaaaaggtg gaaaaatagg tctatttgga ggagcaggag
    60361 taggaaagac agttctaata caagagctta taaacaatat tgctaagcaa catggtggta
    60421 tttctgtgtt ttcaggagta ggagaaagaa caagagaagg taacgacctt tatggcgaaa
    60481 tgagtgagtc tggagttata aataaaacag ctctagtatt tggtcaaatg aatgaaccac
    60541 ctggagcgag aatgagagtt gctttaactg gacttacaat ggcagaacat tttagagatg
    60601 agcaaggaca agacgtttta cttttcgttg ataatatatt ccgtttcaca caagctggtt
    60661 cagaagtttc agcacttcta ggacgtatgc catcagctgt tggttatcag ccaacattag
    60721 ctactgaaat gggtgcactt caagagagaa taacatcaac taagaaaggt tcaataacat
    60781 ctgttcaagc agtatatgta ccagcagatg acttaactga cccagcacca gctacaacat
    60841 tctctcactt agatgcaaaa acagttttat caagacaaat atcatcactg ggtatatatc
    60901 cagcagttga ccctcttgaa tctacatcaa ggattcttga ccctagtata gtaggaaaag
    60961 aacattatga agtagctaga ggtgtacaat caatacttca aagatataaa gaacttcaag
    61021 atataattgc tatacttggt atggatgaat tatctgatga agataagtta atagttgcac
    61081 gtgctagaaa gatacaaaga ttcttatctc aatcattcac agttgctgag caatttacag
    61141 gaaatccagg tcaatatgtt cctgttaaag agactgtaag aggatttaaa gaaattcttg
    61201 aagggaaaca tgatgactta ccagagtcag cattcttatt tgtaggaaca atagaagacg
    61261 ctgttagaaa agcgaaggga agtatgtagt catggccagt gaattttcat tgaaagttgt
    61321 aactccaagt aatacttttt atgatggaga tgttgaaatg atagtagtta gaactacaaa
    61381 aggagacaaa ggggtgttga aaggtcatat accatttgtt tcaggtatac ctgtaggaac
    61441 tttaaagata aaaaaagagg gaagttttaa agaggcgaaa atagcagaag gattcattaa
    61501 tgtaactcaa gataaaactg tcatattgac agaatctgct gagtggattt aataaattaa
    61561 tttagagtat aaaaagtcat tttgaccaaa aaaacttttg ggtaaaatgg cttttttgcg
    61621 ttttggtgtt aataaaataa cgataaaatt gtaagttttt agtgataata aattcacaaa
    61681 atgtttaaat ttactgccaa attatgttgt aattaataag ataaagaaga tttagatagc
    61741 ttttatgtaa gtgaaaaagt cttcatataa gaaatacata taaaattttt ataaaattta
    61801 aaaaatttgc ttagttgtgt agtataata
//
LOCUS       DAMJZE010000003        60313 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJZE010000003 DAMJZE010000000
VERSION     DAMJZE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.003"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            20..1300
                     /locus_tag="PC541_000116"
     CDS             20..1300
                     /locus_tag="PC541_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2837606.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1563..2954)
                     /locus_tag="PC541_000117"
     CDS             complement(1563..2954)
                     /locus_tag="PC541_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2837607.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2965..4185)
                     /locus_tag="PC541_000118"
     CDS             complement(2965..4185)
                     /locus_tag="PC541_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2837608.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4199..4987)
                     /locus_tag="PC541_000119"
     CDS             complement(4199..4987)
                     /locus_tag="PC541_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2837609.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5004..6278)
                     /gene="larA"
                     /locus_tag="PC541_000120"
     CDS             complement(5004..6278)
                     /gene="larA"
                     /locus_tag="PC541_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2837610.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6396..7109)
                     /locus_tag="PC541_000121"
     CDS             complement(6396..7109)
                     /locus_tag="PC541_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2837611.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7234..8493
                     /locus_tag="PC541_000122"
     CDS             7234..8493
                     /locus_tag="PC541_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2837612.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8773..9339
                     /locus_tag="PC541_000123"
     CDS             8773..9339
                     /locus_tag="PC541_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2837613.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9540..10745)
                     /locus_tag="PC541_000124"
     CDS             complement(9540..10745)
                     /locus_tag="PC541_000124"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2837614.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10848..10954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11046..11414)
                     /locus_tag="PC541_000125"
     CDS             complement(11046..11414)
                     /locus_tag="PC541_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2837615.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11466..12743)
                     /gene="obgE"
                     /locus_tag="PC541_000126"
     CDS             complement(11466..12743)
                     /gene="obgE"
                     /locus_tag="PC541_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2837616.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12854..13144)
                     /gene="rpmA"
                     /locus_tag="PC541_000127"
     CDS             complement(12854..13144)
                     /gene="rpmA"
                     /locus_tag="PC541_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2837617.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13158..13475)
                     /locus_tag="PC541_000128"
     CDS             complement(13158..13475)
                     /locus_tag="PC541_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2837618.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13490..13801)
                     /gene="rplU"
                     /locus_tag="PC541_000129"
     CDS             complement(13490..13801)
                     /gene="rplU"
                     /locus_tag="PC541_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2837619.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13902..15323)
                     /locus_tag="PC541_000130"
     CDS             complement(13902..15323)
                     /locus_tag="PC541_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2837620.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15341..16042)
                     /locus_tag="PC541_000131"
     CDS             complement(15341..16042)
                     /locus_tag="PC541_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2837621.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16044..17888)
                     /locus_tag="PC541_000132"
     CDS             complement(16044..17888)
                     /locus_tag="PC541_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2837622.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18207..19400
                     /locus_tag="PC541_000133"
     CDS             18207..19400
                     /locus_tag="PC541_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2837623.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19612..20432)
                     /locus_tag="PC541_000134"
                     /pseudo
     CDS             complement(19612..20432)
                     /locus_tag="PC541_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(20562..21353)
                     /locus_tag="PC541_000135"
     CDS             complement(20562..21353)
                     /locus_tag="PC541_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837624.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(21357..21686)
                     /locus_tag="PC541_000136"
     CDS             complement(21357..21686)
                     /locus_tag="PC541_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2837625.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22171..22584)
                     /locus_tag="PC541_000137"
     CDS             complement(22171..22584)
                     /locus_tag="PC541_000137"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2837626.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(22625..23755)
                     /gene="rodA"
                     /locus_tag="PC541_000138"
     CDS             complement(22625..23755)
                     /gene="rodA"
                     /locus_tag="PC541_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2837627.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(23840..24124)
                     /gene="minE"
                     /locus_tag="PC541_000139"
     CDS             complement(23840..24124)
                     /gene="minE"
                     /locus_tag="PC541_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2837628.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24142..24939)
                     /gene="minD"
                     /locus_tag="PC541_000140"
     CDS             complement(24142..24939)
                     /gene="minD"
                     /locus_tag="PC541_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2837629.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(24963..25646)
                     /locus_tag="PC541_000141"
     CDS             complement(24963..25646)
                     /locus_tag="PC541_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2837630.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(25744..28722)
                     /locus_tag="PC541_000142"
     CDS             complement(25744..28722)
                     /locus_tag="PC541_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2837631.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(28734..29222)
                     /gene="mreD"
                     /locus_tag="PC541_000143"
     CDS             complement(28734..29222)
                     /gene="mreD"
                     /locus_tag="PC541_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2837632.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29237..30130)
                     /gene="mreC"
                     /locus_tag="PC541_000144"
     CDS             complement(29237..30130)
                     /gene="mreC"
                     /locus_tag="PC541_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2837633.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30140..31150)
                     /locus_tag="PC541_000145"
     CDS             complement(30140..31150)
                     /locus_tag="PC541_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2837634.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(31271..31930)
                     /gene="radC"
                     /locus_tag="PC541_000146"
     CDS             complement(31271..31930)
                     /gene="radC"
                     /locus_tag="PC541_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2837635.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(31975..32559)
                     /gene="maf"
                     /locus_tag="PC541_000147"
     CDS             complement(31975..32559)
                     /gene="maf"
                     /locus_tag="PC541_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2837636.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(32779..33750)
                     /locus_tag="PC541_000148"
     CDS             complement(32779..33750)
                     /locus_tag="PC541_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2837637.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(33766..34341)
                     /gene="rsxA"
                     /locus_tag="PC541_000149"
     CDS             complement(33766..34341)
                     /gene="rsxA"
                     /locus_tag="PC541_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2837638.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(34356..34949)
                     /locus_tag="PC541_000150"
     CDS             complement(34356..34949)
                     /locus_tag="PC541_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2837639.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(34962..35531)
                     /locus_tag="PC541_000151"
     CDS             complement(34962..35531)
                     /locus_tag="PC541_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2837640.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(35531..36508)
                     /locus_tag="PC541_000152"
     CDS             complement(35531..36508)
                     /locus_tag="PC541_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2837641.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(36531..37868)
                     /gene="rsxC"
                     /locus_tag="PC541_000153"
     CDS             complement(36531..37868)
                     /gene="rsxC"
                     /locus_tag="PC541_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2837642.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38084..39028)
                     /locus_tag="PC541_000154"
     CDS             complement(38084..39028)
                     /locus_tag="PC541_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837643.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39139..40413)
                     /locus_tag="PC541_000155"
     CDS             complement(39139..40413)
                     /locus_tag="PC541_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2837644.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(40545..40916)
                     /locus_tag="PC541_000156"
     CDS             complement(40545..40916)
                     /locus_tag="PC541_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2837645.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41144..41743)
                     /locus_tag="PC541_000157"
     CDS             complement(41144..41743)
                     /locus_tag="PC541_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837646.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(41944..42156)
                     /locus_tag="PC541_000158"
     CDS             complement(41944..42156)
                     /locus_tag="PC541_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2837647.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(42229..43944)
                     /locus_tag="PC541_000159"
     CDS             complement(42229..43944)
                     /locus_tag="PC541_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2837648.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(43944..44498)
                     /locus_tag="PC541_000160"
     CDS             complement(43944..44498)
                     /locus_tag="PC541_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2837649.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(44491..45093)
                     /locus_tag="PC541_000161"
     CDS             complement(44491..45093)
                     /locus_tag="PC541_000161"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2837650.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45102..47750)
                     /gene="polA"
                     /locus_tag="PC541_000162"
     CDS             complement(45102..47750)
                     /gene="polA"
                     /locus_tag="PC541_000162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2837651.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            47902..47989
                     /locus_tag="PC541_000163"
     tRNA            47902..47989
                     /locus_tag="PC541_000163"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47936..47938,aa:Leu,seq:caa)
     gene            complement(48176..48619)
                     /locus_tag="PC541_000164"
     CDS             complement(48176..48619)
                     /locus_tag="PC541_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837652.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(48622..48819)
                     /locus_tag="PC541_000165"
     CDS             complement(48622..48819)
                     /locus_tag="PC541_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2837653.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(49011..49877)
                     /locus_tag="PC541_000166"
     CDS             complement(49011..49877)
                     /locus_tag="PC541_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2837654.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(50103..50972)
                     /locus_tag="PC541_000167"
     CDS             complement(50103..50972)
                     /locus_tag="PC541_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2837655.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(51478..51699)
                     /locus_tag="PC541_000168"
     CDS             complement(51478..51699)
                     /locus_tag="PC541_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837656.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(52200..53180)
                     /locus_tag="PC541_000169"
     CDS             complement(52200..53180)
                     /locus_tag="PC541_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837657.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(53344..54216)
                     /locus_tag="PC541_000170"
     CDS             complement(53344..54216)
                     /locus_tag="PC541_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2837658.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(54380..54931)
                     /locus_tag="PC541_000171"
     CDS             complement(54380..54931)
                     /locus_tag="PC541_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2837659.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(54951..55853)
                     /locus_tag="PC541_000172"
     CDS             complement(54951..55853)
                     /locus_tag="PC541_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2837660.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(55868..58240)
                     /locus_tag="PC541_000173"
     CDS             complement(55868..58240)
                     /locus_tag="PC541_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2837661.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(58461..59150)
                     /locus_tag="PC541_000174"
     CDS             complement(58461..59150)
                     /locus_tag="PC541_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2837662.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(59391..>60313)
                     /locus_tag="PC541_000175"
     CDS             complement(59391..>60313)
                     /locus_tag="PC541_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2837663.1"
                     /translation="FSFFRKSIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERF
                     GSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYL
                     SIQESHMRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMIL
                     DIIINEKSEVCLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENI
                     NKYIRIKGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKY
                     NGELLFENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT        18917 a        11137 c         6448 g        23811 t
ORIGIN      
        1 aagataaaag gagtatttta tgaacatact tggattaata gttgaatata acccatttca
       61 taatggacac atatatcatt tatctaaatc tatagaaaaa acaaatgcaa cacatactgt
      121 agcaataatg agtggaaatt ttctacaaag aggcgaacca gctttatttg ataaatacac
      181 tagagcaaaa attgctgtcg aaaatggtgt agacttagtt atagaattac caactttatt
      241 tgcttgtcaa agtgctgaaa ttttttctca tggtgctata acaattttaa attcactaaa
      301 ctgcataaat tctatatgtt ttggtagtga agaaggaaat acagaaattc tttataatct
      361 agctgaaatt ttagcaaatg aaccaaatga atttaagtta cttttaaaaa aatatttaga
      421 tgatggtctg ttatttccaa cagctagagc taatgcactt tttgattata tacatcaaga
      481 caaaaaaaat aattttatac aaaattattc aaaggaacaa ttatcaaaaa cattaaattc
      541 atcaaacaac atactgggta tagaatacat aaaaaacctc atccatttaa acagtagtat
      601 aaaaccattt actatctcca gggtccaatc agaatataat tccgaagaaa ttgagagtga
      661 tatttgttct gctactgcta taagaacttc acttaaagaa aatacagacc tttttcattt
      721 aaaaaatgtt attccagaaa aaacttataa catcctaaat gaaaaaatag ataatggttt
      781 tttacctatg tttgatgact tcttctttga tattttaaaa acaattatac ttagagatat
      841 ggacacttta aaagagtatt ttgaagtaaa tgaaggcata gaaaacagaa tctataaaga
      901 tatctttaca tctgaatttc tacatgaact acaattagca attaaatcca agcgatacac
      961 tttaaccaaa atcaaacgaa ctctaaacaa tatattgtta ggtattaaaa aaagcgacgt
     1021 gcaacttata aaaaacatag atactatacc ttatataaga atacttgctt ttaatgataa
     1081 aggtagagaa attctaaaag agataaaaaa taaatctgaa ataaaaataa taaacaagtt
     1141 ttcaaatata tcattttcat tagatgatac gatttttaaa acactcataa gttatgatat
     1201 aaaagctaca aatatatata attcaatata ttataaaaac aataaagatt tagtaaaagg
     1261 ccctatggat ttttatactc atccaatata tgtaaagtag ttttttaaaa taactatatt
     1321 tataaaaatc attttattaa ctctttatta tataagatac aatagtctta ctttatttat
     1381 gtacacatgc ttttaggatg acaacatcaa cttaaatatt atgttacttt ctaaatttaa
     1441 gtaaatattg ccatcctaaa aattttacaa tttagcttca gatttaaaag taaagtttaa
     1501 aatattatat ttaaatctaa tttaatattt tttgttttat aaatattatt aaattaatat
     1561 ttctaatata ttttccctgg atttaaaata tttttagggt caaaagctaa ctttatattt
     1621 ttaattaaca tcatataagc atctgaattt gattcatgta agtactcttt tttagcatat
     1681 cctatgccat gttcaccaga aacttgacca ctaagttctc tagatttttt atacatatag
     1741 tcaaaagttt cttttaatct tattttccat gtattatcat ccataccatc ttttaaaata
     1801 tatatgtgta gatttccatc tcctgcatga ccaaaacttt ttattcttat ttttaatttg
     1861 tcttgaagct cgtgagtgta tcttataaac tctgctatct tatctcttgg aacaacgaca
     1921 tcacattcat ccatttgtgt cgtagatgcc tttatagctt ctaaaaatgc acctctggct
     1981 gaccatatag aatcgtttct ttcttgtgta tctgatataa atacatctaa tgcaccattt
     2041 tctaaacata aattagctac tttttcatat tctttttcta tatcttcagt gctatttcca
     2101 tcaaatgtaa gcaaaagata agcatctgat gtattatctg gaaacttttt acctaaaaat
     2161 tcttctgctg ccaatatcac atctctttcc ataaattcta tagccgttgg tattgatttt
     2221 gattttatta ttttaggaac agtttctatt gccattgata aatctggaaa tggtataagc
     2281 aaacttatac ttttctttgg taaaggaagc aattttaata tagctttagt aactattcct
     2341 aaagtacctt ctgagcccac taataaatcc tttatactat atccagaact atttttaacc
     2401 acctttccac ctacatttat aatttctcca tttggtagta caacttcaag tcctcttaca
     2461 taatcccttg taacgccata ttttactgct ctcataccac ctgcattagt gtttatatta
     2521 cctgcaattg tagcactttt ttcacctggg tcaggtgggt aaaataaatc tctatcttga
     2581 acatattgac ctattgtcat caataaaaca cctggttcta ctgtcaatgt taaattttca
     2641 taatcaactt caagtatttt attcatttta catagattaa tcattattcc accatttatg
     2701 gcaacagcag cacctacaag accagtacct tgacctcttg gtgtgactgg tatattattt
     2761 ttatatgcgt atttcattat tttagatact tgttcagttt caagtacatt tactaaaact
     2821 tcagggtact tctcaatccc tccaagttca tcatgtgaaa aatcttcatt tatatcactt
     2881 ccaactaata cattttcttc tccacaagta tctattaaaa aatctatatc ttttttatcg
     2941 attaatttat acatttctat ccccctattt agctgctgat gccatatatt tttgattatt
     3001 atctttgtta ttttctattt tttcaatcaa tttaggaata atatcataaa tatcacctat
     3061 taaagctaaa tgtgctacat caagtatagg cgctttatca tctttattta ttgctactat
     3121 atagtcagca ccctgcatcc ctgcaacaaa ttgaactgct ccagatacac cacaagttat
     3181 tatcaattta ggttttacag ttctaccact aagacctatt tgttttttag catctatcca
     3241 acctgcttct attacaggtc ttgttcctgc cacttgagcg tttaacttat cagctaattt
     3301 atatatcatc tccatatctt cttgtttttt tattgctcta cttgccacaa ctatcacttc
     3361 agcttcttcc agaccaactt ctttattttt ttcttttaca ctcagtacct taattttaga
     3421 ttttagttct tttttttcta ctttacataa agttatcttt cctgtagtat tctctatttt
     3481 ttctggtgct gaaaatattt tatatcttac agtggcaaat tgtggtctat tattaggtgt
     3541 gtttatatga gccattatat ttccaccaaa tgctggtctt atttggtcta aatcagtatt
     3601 tgattgaata tcaagtattg tacaatctgc tgtaagacct gttctaaatc tagcggcaag
     3661 tcttggagct aatgacctac ctaatgtagt tccaccaact aatactatcg ttggttttat
     3721 tttatctata aagttttcta ttgcttttga atatggttct atcctaaaat ctttaaattc
     3781 atcttcatca tatacaaata cttcatccac cccataagaa atcaaattac tgcacttatc
     3841 ttttatgtct tttcctacaa atacacaata cacaggttgt tttatttttg atgccatctc
     3901 acgagcttta cctataagct cgtacgttac aggatgtata tttccattat gatgttcaac
     3961 atatactgca attcccctcc attcatcttt attaatttgt actttactac tctctataaa
     4021 ttcaaatgcg ccttttggtc cacttttgac acacaacttg cacatcttac agccagcact
     4081 tatttctact atacctgact tttcttctat agccccaaaa ggacatattt ctaaaattct
     4141 ctttaagtca gttattttat cttgatttac aactatcttt gacatttatt actccccctt
     4201 acaccaactt taattcctct aatttattta caagtgcaaa acttagttca tctgcatttc
     4261 ctcttacaat atttttatct gtatttggct ttggaggaaa tattcttaca acttgagtag
     4321 gagaaccatt aagtccatag ttattttcat ctttatcttc aaagtcatct aagctatata
     4381 caggtatttc tctatcttta gtattaagtt ttaatttaaa tgaaggtagt cttggttgaa
     4441 aaatgtcttt ttctacagtt attaaacatg gatattcaat ctcacatact tgtaaatcat
     4501 ttggcatatc tatttcaaca actatacttt catctttaac ttcaattaat tttgtaacgt
     4561 tagtaacatg aggtatatct aaaaattcag ctacttcagg tcccacctgt gctgtatcac
     4621 catcagtagt ttgttttcca caaattatta ggtctacctt tcccatcttc ttaatacctt
     4681 gtgatatggt ataggaagta gccaatacat ctgctcctcc aaatctccta tcacttatta
     4741 atgctccttc atcagctccc atcataaaac tttcttcaat taccttcttt gcctgaggag
     4801 gacccatgct aagtactttt agattcgctt tcttatcttc ctttattcta agagctgttt
     4861 ctaaagcata taggtcatag ggattcattt ttgaatctac tccatctctt tttagtactc
     4921 ctgtagtttc atcaacttct actgaagtcg ttccaggaac ttgtttaata catgtcaaaa
     4981 tttccataaa gtacactcct tttttattta actataactg atactccatc aggtataact
     5041 actactttgc tctccttacc aactatacta ttcgccattt ctaaagcttc tttaaagtta
     5101 aatgcatgtt tcatatgcat tttttttatc atttcaggat tacacttatc tgttaccatt
     5161 atcacagtat atttagataa tattctcgct agtatctgaa attcccattg gtctggaatc
     5221 gtatctactc tatcgacact tctaatttta tctaaaagtt gttttggaga atctgcattt
     5281 gctacatttt cataaaaaga ttgacctcca tgaccatcat tacatgctga aatcattatt
     5341 attactccac cgtctttaca agtagcttca gctgctgtca ttcctttaac agattgataa
     5401 atattttggt ctagtggata tccaccattt gtagttacaa caatatcaga ttttatagaa
     5461 tttacttttg ataactctgt tacaaatcta catcctttag tatgtgcttt ttcgacatca
     5521 ccagcaaagg aagcaattat ttttttatct ttatctatta caacattcaa tatgaatgct
     5581 aattttgcaa ctttagctgc atataccata tcctcatgaa taggattatt attcaatttt
     5641 ccagttcttg aattatcact atctataaag tcactacaat gattgtacat tatagtcttt
     5701 gcagatgcga ttcctggaag tacacttttt ctgccacctg aaaaacctgc aaagaaatga
     5761 ggttctataa aaccttctgc tatcaaaagt tctgcctcat atgctagttt gtttatatat
     5821 aaatctccac ctgaaggcaa tatccctact ttacacatag atttatcatc agtagatata
     5881 tgcataacta tatcttcatt atctactatt tcttctccca ttttataaat tagctcttcc
     5941 cttgtacttg gcctatgaaa tccagttgcc actataatct tcacatcaat ttctggatta
     6001 acattcctaa tccttcttaa tataattggc atagttattc tacttggtac aggtcttgta
     6061 tggtcacttg taattatgac tacattcttt ttccccttaa ccagttcttc cagagaatta
     6121 gagcctatag gattattcat agattcttca actatctctt cttgagtctt atgttctata
     6181 taattatccg attttgactc caaaacaccg agaaaacgtt catcaggtat ttctaaaatc
     6241 attccttgtt ttgaatacgg tacttttaac tttaccattt ttaatctccc attataataa
     6301 actataaaat tggtatgtgg tcataccagt tttacattta tatgatagta ccattatcta
     6361 ccatttgcaa gaataattta ttttataaca aacttttaat tttcattttt tctataattt
     6421 ttacttataa aatccatatg ttcctttgct ttctcacatg caagttctac atcattttct
     6481 acaactgcat tgtatatagc ttcatgctgt ttaagcaata tattttcatt aaaaggttct
     6541 gcgattattt tttctcttgc atcttctata aatccatcaa ataattttga tgctgtcata
     6601 aaaagacttt gaattaaata atttttactt aatgttgcta gtttactgtg aaatttttgg
     6661 tctaataaca ccagtactcg attattatct tttttattag tttctaccct catttcatct
     6721 attatatgtt tcaattctat aacttcagaa gaagttattc tctttgaagc aagttttgca
     6781 atctcaattt ctaggattat tctaagctct aatatatctt caagtgtacc attattaagt
     6841 ttaaaaatca tagataaagg ttctattaaa gttttttcta tatttgtaca tataaaattt
     6901 ccttcaccct gtctgctttc tatcactccc atagtctcta ataccctaat agcttccctt
     6961 atggaagttc tactcacatt catttgttca gacagttctc tttctgatag taatttatcc
     7021 ccctttttta actctccatt tagtatttta tattgtattt gctcaattac ttgttcataa
     7081 acttttttac tagctatact attaaacatt ttttcctcca atatgataat cgttgttgta
     7141 atatatatct attatatatc ttaagccatt acattcatat atttttatta cttttaatat
     7201 aatatcttat aactcaaatt tacaggtgat attatgaaaa aaaataaaat tattgctttt
     7261 tttgctccat ctatcatagt tggagttcta gttttaggga ttatcctata cccaaatgaa
     7321 gctataaaat ctgcaagaga aggcttttct atatggtcta atgttttagt tccatcacta
     7381 ctaccattta taattggagc aaacttaatt gttgatttaa aaatagtgga tattataggg
     7441 tttatcatca atcccataac tagatttgta tttaatgttt caggaaagag cgctttagct
     7501 tttgctatat ctacagtatc tggttatcca gtaggagtaa gtttagcttc tgaatttagg
     7561 agtaatggac aaatttctaa gtatgaagca caaagacttg tgtctttttg ttctacatca
     7621 ggacctttat tcataatagg ttcggttggt acaggtatgt tccaaaattc aaacttaggt
     7681 tatttaatga ttctctgcca ttatctagga actatattgg ttggtctttt ctttagaaat
     7741 tatggtaatg aaaatcttcc aaagacaaaa ttggatttaa aaacaaatat aaaaaatgta
     7801 atcaacatta gaaattcaag tgggaatggt ttttttgttt tatttggaaa tgctgtattt
     7861 aatggagtaa atacattact tacagttggt ggatttgtaa ttgttttttc agtagtattt
     7921 aaaatactat ctctttttaa tgtaatctca ttaatagcat cagttatata tttgcctctt
     7981 tcatttttag gtgttagtaa agaactctgt caagcatttg taagtggtct atttgaaatt
     8041 actataggat gcaataaagt atctagtata acaagttctc cagaaatttt aagagcatct
     8101 ttagctagtt ttttgatagg atttagtgga ttatccatcc tagctcaatg ttgtacattt
     8161 ctatcaaaaa cggacataga tttaaaatta tacatactaa gtaaatttgc gcatggagtt
     8221 ttagctgcta tctttatatt tatactttat ccaattgcaa actctgctgt gttggtttct
     8281 agctttgctg atacatacaa cgttatatat aataatttaa tctggtttta ttatcttagc
     8341 tattatgata caattcttca aattgtagta ataatttatc taatctcagc cgtatttatt
     8401 gcaaaaaaac atatgaaaac tttatctaca aatgggaaac taatgcgata taaaaatact
     8461 tttttcacta agaaattttc acgaaaaaaa taaaactaaa aataaaacta ataaaactta
     8521 actaaatact tccatttttt tattatattt gtcataattt taataacctt tttaattagt
     8581 ttaacagcat agttatttca tcacaattta ataattttat tagaaatatt tttcaaaaaa
     8641 ttctccttaa tcacatatat cacacttgta taagtttact gggaaatttt tccattatat
     8701 aatatttcat ttatattata taataaatac cgacctaaac ttaccaaatc aaactaagag
     8761 gagtggttga aaatggcaac aagacctata gaaatagacg aatataaaaa gataatggaa
     8821 ctattacata ctggctttac ttatagtgaa aatggcgttg aaaaaagatt tagaaaaaat
     8881 cctaaagttg cattggcact aatgttagaa gctaatttag gtctaagaat atcagatatt
     8941 ttaagattaa agataggtaa ttttaaaggt aatatgttgg aaataaatga aaaaaagacg
     9001 ggcaaattac agtatagacc agttaataaa aatataattg agtccataaa tgaacatgca
     9061 agaaagtata atcttaaatc aaatgactac ttagtaaata taaagacaaa ggctattcaa
     9121 aaacagttga ggataatttg taaatactta aatctgtata atataagtac acatagcttt
     9181 agaaagctct atgctactac tcaatttgaa aagagtaaca ataatttaga gttggttaaa
     9241 gaacttttaa atcacagctc tgtagccact acacagcgat atataagggt aactcagcaa
     9301 gctattaatg aagctagtga aaatttcttt attggttaat tataacgtat acatttttct
     9361 attatcttat taattcacca gatattaaaa aagagtgtct caaaatgaac tttttagttc
     9421 atgagacact ctttttttat aaaatcaaat atatttttat tatttcaaca ctggaaaaaa
     9481 ggcttatctc tctattttga gacaccctat taaattttca taattcttgc ataaacatat
     9541 taatctatct tcaataattt tttagctgat tcactgtaat tatttctttt tacaatttcc
     9601 aaaactctat caatctcatg ttctgacttt gcacttacaa aaatcgccaa ttcttcatca
     9661 tctataatat cataactatc tacatattta tctaaacatt ctaaaattcc atctttatta
     9721 ttatacccac caaatctaac tactgcacta cactcatctt ctataagtcc acttaaatcc
     9781 aatttccctt ttgaaaaaga ctttatattt gaacttctat tatctattat atctataata
     9841 tctgcataaa cagcacttcc tgttgcacct cttccagctc ccttacctac aaaagaaagt
     9901 tcacctactg aatcacctac taaaataact acgttgaact cattatctat ctttgaaagc
     9961 atattattat tgtccactaa tgctggtcta acaaatccac ttacttctcc tgattcatac
    10021 ttacttctag caaccaattt tatcttacaa tttaattttt ttgcatattc tatatctttt
    10081 tcatctattg aagttattcc ttctaataat aaatctttcc aatatactat ttttccataa
    10141 gataaagtag acaatataga taactttcta gcactatcat atcctaaaac atctgcatct
    10201 ggattaactt ctgcaaatcc caactcttgt gcctgtttta aagcgacatc atacggtaaa
    10261 ttttcatcat acatctttga taatataaaa ttacaagtcc catttaaaat agcgtttatg
    10321 ctctttatat tgttgccttc cagtgattct atcaatggtt ttaaaactgg aataccacct
    10381 gccacagatg cttcaaactt gatgctaaca ttatttaagt tagctaattc tataaggtca
    10441 ctaccttttt cagccagtaa atctttatta gctgtcacta catgtatttt tttatttaac
    10501 gctcttgtta cataatcaaa agctggattt aatcctccaa ttacttcaac tagtatatca
    10561 atatccttgt caaatatatc ttctatatca gtagttatct tatcaaaata cttcttatac
    10621 ttgtgttttt ctttattttt aactaaaatt ccaacaactt ctatatctct ttttgaagta
    10681 ttattttcta ttaaatcaat taatccactt cctactgttc cataccctaa tattgctatc
    10741 ttcactgtaa cataccccca tcattttgat tatattaaat tatttatata tataaatata
    10801 ccaataaaaa aagcttgagt ataaactcaa gccatatgac gggtttatac tcatctttca
    10861 gcataaatgc tgcaggattt agcaccaatg tataattaaa tatactggtt gccgggcttc
    10921 acagggccag tccctctgcc actcttgata agattgctaa tttaaattta tgtttaatag
    10981 tataatacaa tttcttccaa ttgtcactat ttttttaact tatttcccta aaaggatata
    11041 aactattatc taacagctct tttagttggt ttaccctttt ttgtaacatt attattttta
    11101 gtctttgctt tcgcctgttc atgtcctgga aaaagtattt taggctcttc aatattcctc
    11161 ttgtaaatag taaatttaaa accaatagcc tgcacaaatt cagctcttaa agcttcacat
    11221 actgcattag cagtttcttt agtatcaaga cctgcatttt ctaatatagt aacttttact
    11281 aattctctag ttcttaacat accatctaat tgctctaaga aactctctgt aacaccttct
    11341 ttaccaattt gtgtagtagg ctttaaagta tttgccaaag atcttaaata cgccctttgt
    11401 tttcctttta acatattctc acctctataa ttctatactt aaattatcta taatctatat
    11461 ttaaattaat tgtaaaactc aaattctaat tcataaattt taaccacatc tccatcttct
    11521 attcccattt ctctaagcct atcaaaaaca ccttgactct ccatagcttt ttggaaatat
    11581 tgaagggatt ccatatcatc aaaatttaca gaatacatta ttcttctaag tgctttacct
    11641 gtaactacat acacaccatc ttcaatatct atacttaact cttcttcagt accaatatca
    11701 agttctggtc tatacatttc ttcttctgaa actagctcca catcttctac ttcttttagc
    11761 attttagaaa cataggctat tacttcatct atgccttgac gagttgctgc tgacatttta
    11821 aatactgtat atcctctgcc ttctaattca ctcttaaact tctcaaactt actctcatct
    11881 tctaatatat caaatttatt tgcaactaca acttgtggtc tctttgaaag cttttcattg
    11941 tacaatttaa gctcatcatt tatcttgtca aaatcttcaa tagggtctct tccctctaat
    12001 ccagatatat ctacaatatg tatcaatacc tttgttctct caacatgtct taagaactca
    12061 tgacctaatc ctatgccttc agaagcaccc tctataattc caggtatatc tgcaagtaca
    12121 aaactatctc caaacttagt ttgaacaaca cctagatttg gtgtaagtgt tgtaaaatga
    12181 tagtttgcaa ttttgggttt agctttagtt acaactgata gaaatgttga tttacctaca
    12241 tttggaaatc ctaaaagacc aacatctgct atcattttaa gttcaagagt tatccatctt
    12301 tcttctccat ctgtaccaga ttttgcaaaa gctggagctt gtcttactgc atttgcaaag
    12361 tgttggttac ctttaccacc tataccacct tttgcaacta tagctgtatc tccttctttt
    12421 tttaaatctg ctaatacaag acctgtagcc tcatctctta ttactgtacc tgctggtact
    12481 tttaatacta aattttctcc atttttacca gctctttttc ctttgcttcc gtcgccacca
    12541 ttttgagctt gatatttttt ctggtactta aagtccatta gagttctaag ccccaaatca
    12601 acttcaaata tgatgcttgc tccacgacca ccatctccac cgtctggacc accagctgga
    12661 acgtattttt ctcttctaaa tgctaccgaa ccatttccac catttccagc cttaacaaat
    12721 attcttgcct tatctataaa caaattcttc acctacttaa ttaaaaactt ttaattagca
    12781 ttaccaaaaa taccattata aattcatcag tattataaga aaaaggagtg cctcaacact
    12841 ccttttatgt atattactca gctattgcta ctggatatat actaactttt tttctcttct
    12901 tgtcttttct ttcgaattta acagtaccat caactaatgc aaataaagta tcatcactac
    12961 cttttccaac attagttcct ggatgtattt tagttcctct ttgtctaact attatacttc
    13021 cagcagttac taattgacca tcgaatctct taacacctaa tcttttagat atagaatctc
    13081 taccattttt agaagaacct accccttttt tagatgctaa taattgtaaa ttcatgttta
    13141 acatatggag tcacccccta cttttttaaa atttttatat tttttgggta ttcccgttta
    13201 atttctttaa ccgctaattg aaagtgattt agtaataatt gtgcgtcttt tacatgtgta
    13261 tctttgacta tacactctat atgaccttct tctcctacta ctttttcaaa ttcaatcttc
    13321 tgtaatttgt ctaaagaatt tataactgca atagcattag atgtaactgc agcacatact
    13381 atatcatagc ctatttcagc aaaatcagca tgtcccttta aacaaaaacc ttgcaacaag
    13441 aaatcatcat tataataata tttaactttt atcataactt aaccaataac taagcgttta
    13501 ttttttctac aactatctta gtgtatgatt gacgatgtcc ttgttttctt ctgtaatctt
    13561 tttttgcttt atacttgaaa actataactt tcttagcttt accttgttca acaactttag
    13621 cttgaacaga tgctccttct actactggag aacctaattt taattctcca tctttagagc
    13681 aagctaatac ttcgtttaaa gtaacaacat cacctgcgtt agcttctaat ttttcaacga
    13741 ataatacatc accttcagaa actttatatt gctttccacc agtctttact atagcgtaca
    13801 ttaaatacac ctcctcggcc tcaaactcgc cagtacaagg tacttcattt gaagatttaa
    13861 acctttactg tgcggcatta caattattaa ttataaaata tttacatttt attgtcaact
    13921 attttatcga aaataacatt ggttttattg tgctttaatg tactatttgc gtttaaagaa
    13981 atctttatat tatatttttc accaatttta tcaattatat ccatataata ttcttttatt
    14041 tgttcaaaaa taaaatcatt aaattctacc attatgtttt tgtaaacagt atgttcactt
    14101 attctcataa tttctttttc aatactatct agtatataat gtacagattt tattgtttgt
    14161 tcaccatcac aacatggaca agacattaaa taatacttat caatggattc tttttctcta
    14221 cgcctagcaa cttctactaa accaagtttt gtcataccta atacttcagc ttttctttta
    14281 tctttattaa attcttcttc taggatgtgt aaaatctcct tttgatgctt ctttttatgc
    14341 atatcaataa aatcgattat tattatccca gctatgtctc taattctaag ctgtcttaca
    14401 atttcttttg cagcttcaag attagtttta taaacggttt catcaagttt accagttcca
    14461 ataaacttac ccgtatttac atctataacc gtaagagctt ctgttttttc aattattaaa
    14521 taacctccac tctttaacca tactttttta tttaataatt tttctatttt agattctatt
    14581 ctgtataaat caaatacatc tctattttcc tcaagacaca gtttatcaac ataagtctta
    14641 tctatacctc ttaaaataga cttaagttca gagtacttat cataactatt ggtaattata
    14701 gattcaatat catcattcac attatctttt acatatttag ttgcaaaatc taaagactta
    14761 tataataatt tcggacctat accaagttta tattctttta aaatgttttc atactttaat
    14821 ttcaactctt ctatatcttt ctttatttca tcatgagtgc aaccttgagc ctcagtcctt
    14881 attattaatc ctagattttc cttatttact gccttagtaa ttttttttaa tctaaatcgt
    14941 tctttttcat cagtaatttt atttgatatt gtagttctat cattggaagg tatatagaca
    15001 atataccttc cagaaatact aatctcagtg tttaattttg cgcctttagt tcctatttct
    15061 tctttattta tctgaaccaa aatatcctga ccagatttta tagtacttcc cttttttaat
    15121 tgtaaataag caagtttctt aaatccaata tctacaaaac aagcttctat tcctggtata
    15181 acttttttta cgattccacg atatatattg gatacagtct tcttatttaa attgtcttca
    15241 acaaaaagtt ctgttaatct ttcatcttca agtactgctg ttttctggga ccctattagg
    15301 gactcaataa ctatcttttt cacaacttca cctctcgagc ttacatagga gtcactaatt
    15361 ccccatcttc taatacatat aaatctcttc tcaatatatc aacatctaat gggtcaatat
    15421 caagttcaaa catctctaat atctttggta taagtatatt agtatttaaa ttagtttttg
    15481 agcctgtagc tatagtagca gtaagagtaa tatgttcatc ttctaaattt aaaacatcaa
    15541 aggttcttat cataggtctt atatctactt caaccatctt acctttttta ttcttttttg
    15601 ttatgattat ttctttatta ttcataaagt caattacttt tctttttaca aactctttcg
    15661 tcataggtct tttcaaatct atattaaaca aatattctcc atagtctatc acagaagaaa
    15721 gagaaggtgt ttgtctatca atttcttttg ctttgataaa atctatccca tctggaagtt
    15781 gaattgatac tttatttaaa aattcctcta ctgataaatc atcttcttct atttcaatat
    15841 ctacatactc accttgactt tctgccccta aagccaaagc attgccataa ctcattttag
    15901 ggtgaggatt aaatccttga gaatgtgata agtttatttc agctcttcta aacgctcttt
    15961 gtaacaatct ctgaaggtca agatgtgata tatatatcat gtcgccttcc tttttaaatt
    16021 ttactcttat tatcttactc atattagcaa agcccccttc ctatatcatg tatatttatt
    16081 ccacaaccat tgcagttatg tctacaatca gaagtgattt tttctttttt agcattttcg
    16141 tcttctctaa ttaagaattt tttactaata cctacatcta tatggtccca aggaaatacc
    16201 tcttcatagt ttctatttct atgtgcataa aaatctatag aaagattatt cttttccata
    16261 gcttctttcc atatatctaa attaaaatgt tctgcccatc catcaaattt agcacctagt
    16321 ctaaatgcat catatataac ttttcctatt cttctatcgc ctctagctac tactgcttcc
    16381 atcaaactag tctttgaatc atgataattg tattttatat tgttatttct taatttattt
    16441 actaaatgtc tctgcttatt aataacttct tctgtagtat cttgaccatg ccattggaaa
    16501 ggtgtaaatg gctttggtac aaatgtagaa gtactcactg taacactaaa acttctcttt
    16561 agctttcctc catttacttt tctataaata tcaataactt tataagccaa tttagctata
    16621 ccatctaaat cttcataagt ctcagttggt aaaccaatca taaaatacaa cttaacacta
    16681 ttccacccca tctcaaaagc tctttctgtt gcattttcca aatcttcttc tgtaacacct
    16741 ttatttataa catctctaag tctttgagtt cctgcttctg gagcaaaagt aagtccagat
    16801 tttcttactt gttgtatttt atcagcaatt tccattgaaa aattatctaa tctaagggat
    16861 ggaagcgaaa tcccaatatt attagatgca tattcatcta ccaaataatc agtcaactct
    16921 gataacttac tataatcact cgtactaagt gatgataatg atatttcatc atatccagta
    16981 ttcttaacta atttatctaa tatttcttta agtcttttaa cactcttttc tcttattggc
    17041 ctgtatatca tacctgcttg acagaatcta catcctctag tacatcctct aaataattct
    17101 agtactattc tatcatgaac agtatctata taaggtacta ttagtttttc tggataatct
    17161 acagtctcaa catcttttat tatcctttta gtaggatttt tagtaatccc ttctctatta
    17221 ggagttacac tttgaactgt accatcttca ttgtacttga catcataaaa acttggtatg
    17281 tatactcctt ctatgctaga tattttatat aaaaaatctt ctctagtcgt tttatttttc
    17341 ttccattctt tatactcatt tactacttct aaattaacct cttcaccttc acctaatatt
    17401 actacatcta caaaatctgc taatggttcg gaattataag cacaaggacc accaaccatt
    17461 ataaatgggt cttccaaagt tctttcttct tttaatattg gtatattggc taaatctaat
    17521 atatttaaga tattagtgta tgaaagttca tattgaagtg taaaagtgac aaaatcaaag
    17581 tttgttatag gctctcttga ctctaatgca aataatggta tacttttttc tctcattata
    17641 ttttccatat caactgcagg agaatacact ctctcacaaa atacatcttc atccttattt
    17701 attacatcat ataatatatg actccctaag tgactcatac caacttcata taaatctgga
    17761 aagcaatgtg catatcttat caattcacta ttactagtat ctttatgaat tgaatttatt
    17821 tcatttccaa gatatctagc aggcttttct acttttttta agattttttt caagtctact
    17881 ttattcattt attaaaattc ctccaaacaa gctctttatt tttattctta tttataagta
    17941 atattataca cgataaaata ttttctatca aaaatataac taaaatattg aatttaaaat
    18001 acatattatt cacaaaatag aatagctaaa aatatttaag aatattttct attaaatttt
    18061 tattgaaata caattttttt acaaagtatg atatattata aacataaaac tattgtttta
    18121 catgacttat catgacatct tttgtttcat tcacctcaaa atggggtatg ttacttatat
    18181 aatgataaat tttaggaggg ttaaaaatga gtaaagtttt tgaagtaaaa aaagatatct
    18241 actttactgg agtagtagat gaaggtttaa aagtattcga cataataatg gaaactgagt
    18301 ttgggacaac ttacaactct tatttaatca aggacgaaaa aacggtttta tttgacactg
    18361 taaaggcaaa ttttaaagat gagtttttaa gtaatttatc agaggttact gatatagcta
    18421 aaattgacta tgttgtaatt catcacacag aaccagacca tgcaggtagt ttaaaatact
    18481 tattagatat aaatcctaat atagaagttt attgtactaa agctgctaaa ctatacttag
    18541 atggtcaaat aaatagacct tttaactgtc atgtaataaa agatggtgaa attttaaata
    18601 tcggtaaaag aaatcttaga tttataactg ctccattctt acattgggtt gatacaatgt
    18661 ttacttacat tgaggaggac aagactcttt taacttgtga tgcatttggt tgtcactttg
    18721 caagtgttga tgcagaggta gtgaatagtg aagattatct aaaatcagct aaacactact
    18781 atgattgtat agttaagcca tttgctaaac atgttttaag tgctgtagat aaggtggtag
    18841 gactaaacat agaatttgat actatattga cttctcatgg tccaatgctt actaaagacc
    18901 caatggctgc tgttaaaaga tatgttgagt ggtctacaga agctgttaat acaactaatc
    18961 aaaatcaagt ttctatattc tacttatcag cttatagtaa tactttagag atggctaaaa
    19021 agataaaaga aggacttgat aaagaaggtg caaaggctga attatatgat ttagaagata
    19081 tgacattaac agaaatgcac gatacattag tagtgtcaaa agtaatatta ctaggctctc
    19141 caactataaa caaaactatg gttaaaccta tgtgggattt attctctgtt atagacccaa
    19201 tggctaacca aggcaagata gcaggagtat ttggttcatt tggttggagt ggtgaaggta
    19261 taactatggc tgagacttta cttaaatcta tgtcattcaa aatgcctgta gaatctctta
    19321 agaaaaaatt cttccctagt gaagagacat taaaagagtg tatggcattt ggtgcagaat
    19381 ttgcaaaatt agttaaatag ccattaatca aacataatat tatttaaaat aaatgatttt
    19441 atatttaaaa ataaattaaa atggagttgt ctcaaaatta atttttctaa tctaatttta
    19501 agttcaactc cttttttatt ttccaaaatg actaatataa taataaatgt accctttcgc
    19561 acagggtgca ttatttatag tctgttctat ttgtatacta gtatacttgt attaattcac
    19621 gtatctagta tatgctacta gccctattcc agaattagat gttgtttcac caatttctac
    19681 tttaataatt atattatttt tttacacttt tttgataatt ctgctgcaga aaggttctta
    19741 ccagtaaaat ctaaagaaaa tgccttatca tactcaaaag gataactaac aggtttgctc
    19801 tctggtgcta atgcaaccat accatatggt gtattcataa accctattac agtaactgta
    19861 tgctttgatg ggtctaaatt tgaatttttg cttgtaaact taattgtatt tattgatgag
    19921 ttgattaaac tacacggaac tccctcatac atttcaattt ttggacgtag attatttcca
    19981 taaacttcat caagtattgc cccttgttga tctgctatca tagtgtgatt cccaggtcca
    20041 actggcgcag aaggcttagc tggcgtaggt ttcttaacag ttggcgttgg attctgtgtc
    20101 tttactactt cgccaacagc tggatatgct ttcttgtcaa atggtaatgg gtgagtaaga
    20161 ctcttgtata tgatgtctgc tcctgctgat ttgttggcat caatatctac atgatacaaa
    20221 cgtccatcct caaaacgaat aagattccac tgatggtcat ttgttgtact atgtacataa
    20281 taacattcaa ttcccatagc atcacataaa gctttcaagc tcctacacat tccagaacaa
    20341 gcaccttttt tagtaaccaa tgtataagca gtattggctc cctctgctgc gttccagtta
    20401 acatatgttg tgttgttttt aatgtgatta tgtatagata caatccacta tggagaaata
    20461 taaaagaaaa gaatataaca aacaataaaa aataagatga ttcaaatttt attttgaaac
    20521 atcttattta ctctacaaat taaaattatt tggtgaatta tttaattatt attttttatt
    20581 aaagaaattt taaatcttaa tataaaatac gctattatac ataataatcc aaatacgaat
    20641 gctactggaa ttaacatctc cgattcaaaa taaaagaaat attctaagaa aatatctgga
    20701 tttactatat tcccattctt gtctaaaaaa tcttttccat tatatcctaa atgctgtata
    20761 aaattactta tgttattata atctgcttcc ctctcatata ccatattagc attcaatcca
    20821 gaagaactta aattagtgtt tccatctttc atatctatta catatagtct aagagctttg
    20881 actttactat acttatcttt atcaagtatt ttttcaagtt ctaacttata ttcaccaatc
    20941 tttgtcatat catgtctttc tgccaattgc tttatttctc catcaagttc ttttcctaaa
    21001 ttatacatat tatgttgtaa actattattg taataccaca taaatccaga aattaaaaat
    21061 gcaattatac ttatgtaacc taatactttt ttaaacccca ttacaattga tttatgccta
    21121 tccaatgata aagatagttc ttttattata ttacataatt catactgcat ttcatcacca
    21181 tcatcaaatc tttcaattgc ttttttacat gcttcttctt catctaatcc ttctaattta
    21241 aattcatttg cagattctat aagatggcat ctcatttcct gttttaattc tatagtactt
    21301 ttattgtcac attttttata tagtttatct aaatattcat ctattagttt cataaattaa
    21361 tccacctctc ccaaaaattg attcataacc tttttaataa atctccattc ttgtttcttt
    21421 atttctaaaa aatctttacc ttcattagtt aaattataat attttcttct tccacctgaa
    21481 ctttgttcat tgctataata agatttaatt aaatttttag attccattct tttaagtgca
    21541 agatacattg ttccttcttt taattcaaac tcacttcttt ctcttacttg ctttgcaatt
    21601 tcatatccat aacaatcttt cttttctaat atagaaacaa ttaatatatc gatataacct
    21661 tttaacactt ctttatttaa ctgcattatg taccaccgcc ttttcaccta ctactctata
    21721 atgtatagta cttatattta ctataatatt acatagtact ctataataca aggtttatat
    21781 tctacaatag acttaaaaat aataaatagc tgtatattaa tatttaatct ataaatacta
    21841 atatacagct atttatcaaa ttttaaaaat aaatttttgt tttattaatg ttgtcataac
    21901 aacatttact tggttgaaat agtattacct ataagtttaa atatatataa taatagactt
    21961 gattctattt catattatta aaatagagta atagtttaaa ttctactact ctatctataa
    22021 tctatttcct aattatttaa tctcaaattt agctcttcat catttaaatc tttcataaga
    22081 tttctacagg tactagattt attccaacag aaaaactagg gcattaaaca cttatatatc
    22141 tgtttcttta gactgtaata ttaatatttt ttaaatacgt tgacttttgc tttttctaac
    22201 ttctctccat gatagttctc ctctatcaag agcttttata aatatttctg ctgaagctag
    22261 atttgtagct attggaacat gataaacatc acaaagtctt attaacgctt gtatatctgt
    22321 ttcatgagcc tgagatgtca atgggtctct taaaaaaata actaaatcta tttcttgtgt
    22381 tactattaaa gaacctattt gctggtctcc acctaaagga ccagatgcta atctatttat
    22441 atttaattca gtttcatcca ttattctttt tcctgtagtt ccagttgcat atagtccata
    22501 cttttttaaa attgattcat aacctataca aaatccgacc atagtgtttt tcatttggtc
    22561 atgtgctact aatgctatat tcatatatct aatctcctca atttatgttt agatgtattt
    22621 aaatttagaa atttattttc tttcttctca tacacacatt tagtactaat cctaagtttg
    22681 ctaaagatgt caataacgaa cttcctccat aacttacaaa aggtaatgtt actccagtta
    22741 ctggtattaa cgcaaccgtc atacctatat tttggattat ctggtaacca aacattccca
    22801 taacaccaac cactatgagg gttccataaa aatcttttgc atctcttgca attgctaaaa
    22861 gccttaatag gaatatcata aataagatta tcaacactgc cattcctatt acaccaagtt
    22921 cttcacctac aacagcaaat ataaaatcac tatcttgtac aggtaaaaaa tcttcttgat
    22981 tttgacttcc attgtaaagt cctttaccag ttacgcctcc agaaccaatt gcaatcagag
    23041 attgcataac ttgatagttt ccttttagag ttacatcttc tggatttaga aaagcttcta
    23101 ttcttacctt ttgatggtct gccatcatta aatacatcag tggcattgac actaatagta
    23161 tgattattcc tctttttatc aactttgaac taagacctgc tgtgaacagc attgcaaata
    23221 tcatacaaca aaatacaatt cctgttccca agtctggttg tgcaatcaat aatccgataa
    23281 atggaagaga ataaactaca acaggcataa cttcctttaa cgtatttaat ttatcctttt
    23341 tggattcaag tatctttgca taacttagta caaatgttaa tttgactatt tcagatgtct
    23401 gcaagtcaag tggtcctaag ttaatccacg acctagctcc accttttact gtcccaattc
    23461 caggtaataa aactatggct aacaaaataa gacttattat ataaagcgct ttataatacc
    23521 tgcctagaag gttatagtca aaaaataata tgactataat cattcctata ccaagtacaa
    23581 aagctaatcc ttgtttaatc aattgattat atgaacctgt tgaattagca tgtgttgcac
    23641 tactaagtat aacaagccca aaaataaaga tagctaatac tgtaactata agtttccagt
    23701 ctaactgttt tatcaactta attgtcgact tataattaat attgattttt ttcaaagaat
    23761 caccgtcttt ctatctttaa acatataatt aaataaaagg ggcatatacc aataaggata
    23821 tccccctttt aagcactaat tacatacatc tatcttttat attctttagt ggtatattag
    23881 caactaatgc agatacaggt ttgtcatcat gctcacctct gcatttagac atttttattt
    23941 caagaccatc atcttctatt tctgcataat tagctattac tttcaaaatg tctgttttta
    24001 tcatctctaa aagttgtggt gaacaatcta ctctatcatg aactaaaact agctttaacc
    24061 tctctttagc aacagattta ctagttttag cctcgttaga aaaaactcta aataaatcta
    24121 acacagatgt atcctccttt gctacttagc catgctaaac atttttttaa gtctaccaaa
    24181 gaatccatct tcaacttcaa gatcaagtag aggaacttct tcatttagta tcctttttgc
    24241 gatatttttg tatgcttgac cagcaagtga cttagaatca agtatagctg gctctccttt
    24301 atttgttgat ataattatgc tttcatcatc aggaacaaga cctaacaaat ctattgctaa
    24361 tatttctatt atatcttgtt tatccatcat gtctccacgc ttaaccatat catttctaat
    24421 tctatttata actaatctta tctcttttat ttcatttgct tctaatagac ctattattct
    24481 atcagcatct cttactgctg atatttctgg attagtaact acaatagctc tatctgcccc
    24541 agctactgca tttttaaagc cttgctcaat acctgctgga caatctatta ttatatactc
    24601 gaaagattct tttagtttct cacacaggtc aatcatctgc tcaactgaga cagcattttt
    24661 atctctagtt tgtgctgctg gtaataaata tagattatca aatcttttgt cttttattag
    24721 agcttgctta agcctacaag ttccttcaac tacatctact atgtcataaa ctattcgatt
    24781 ttcaagaccc attactacat ctaagtttct aagtccaata tctgcatcca caactactgt
    24841 ttttttattt tctaggctta gcgcagttcc taagtttgct gcggtagtag tttttccaac
    24901 cccaccttta ccagatgtta taactataac ttcgctcata atactacccc ctatttttct
    24961 ttctatttgt ctaactttga caaatagctt tcgattacaa ttcttccatt acttacaaag
    25021 gctatttctg ggctaatttc tgattcactt tcataatttt cttcatcatc tggagcttct
    25081 gcaatattat ctgctatttg taatatttta ggattaagat ttccagcaac aacatatgca
    25141 aattcattgc cttctgcacc tgcatgtaca aaaccttcta ttttacccat tacaaaaaca
    25201 ttacctcctg ctacaacctt agcaccagca tttatatctc ccataacaac tgtatttgat
    25261 tttgaaagga cttttgaacc aggtttcata tcattcaata taactacatc accttcaaat
    25321 tcaatatttt caccagatct tagggtattg acatattttg ttggaaaatt actattttct
    25381 tcaatttttt gatcttcaac aaattccaca tcaaatctag aagtaattcc ttctttaagt
    25441 tctagaatct ccatgtcagt taaacaatca ctattaattt tactaatttt agccccatta
    25501 aaaaagccaa catatgcttc caatttgttt attatttttt cctgtatttc ctcaaatgga
    25561 gcttctcttt ttatatttac tattatccct cttttattac ccttgaactc tactagttct
    25621 tgactacaaa tttctcttaa agacataaca acctccagag tgatatatca tttatattct
    25681 tcaaatacgc tttaaaccct tttatttttt ctatataaag ctaaagttta tacatagatt
    25741 gagctatcta ttaatttgtg actcgaaatt tatattttcg tcagaagaat tgttaacatc
    25801 attcgtcttg ttgtcatttt tactattagt tggctctaat cccatataag ttccaataac
    25861 ttctctagtt ggtaaaagtg agaatacact tgaatcacct tgaggaatta cacatacaac
    25921 agctatttct gggtcatctg ctggagcaaa agcaacagtc cacgtaaaag aaccataatc
    25981 ttgtttaaat ctatctattt ggtcatctgt tatttttgga tttaattctt ttatagcttt
    26041 tcttagatat gaagaattca ccttatcagt gtcctctaat ctaactttta ctccatcttc
    26101 taattcttcc tctaatttct ttctttcttc gtctttcaaa tctttatttt ctaatttctt
    26161 ttttatttcc ttttccttag ctaatgaaag ctctttttct ttttctgctt tcatcttatc
    26221 agctagttta atcgcatcat ttaggttgac attataagat gacatatgac tttttaaata
    26281 ttcatactcg ttatctgtag gtattttccc acttttctca gctgtaccag tttttgctgc
    26341 tacatctatt ggaaaatcag caaaagcact cttcgctgta ccttgtcttg caaccaattt
    26401 catacctttt gttaattctt ttaagttatc tggattttta aaaggtattt tttcgacttt
    26461 cttctggtca ttaattttaa ctgatgaata atcacttgaa acagctctat caactacaga
    26521 taattctact aaattacctc catttgctat agcagctaca tatcttgata tttgagctgg
    26581 tgtataagca ttttcacctt gacctatagc taagttaaat gtatctgcag tactccattt
    26641 tgcaaagtta aaataactaa atactgctaa atctgctact tcttcaattc tatcttcttt
    26701 aacatccaat ttttttagcc tattcattgt ttcaactcta ccaggagttt tcttttctgc
    26761 tgcccagctt acaatctcat tgattctttt ctcatattct ttaggatttt tttctctagt
    26821 tatatcaaca aaatcatttg ccatttctct ttctaaattt gactttagaa gagcttgtgt
    26881 tgatttcaat ttatcttctg tgctaggaac tttacctgct ctttcttcta cttcatcttg
    26941 aagccctgta ttttggtcaa gcccaaataa ttttgcatac tcaagtattt tatttgcatt
    27001 catatctata ccaatgcttt ttccatctgg ccatgtttta cctgtaccaa ttgttgccat
    27061 atatatatta caagactctt gtattgcttt atataagttt gtcataccat ggtttcctct
    27121 accatggttc catacatagt caccaaaaga ttttttacct aacattataa ccccagggtc
    27181 atttatagta aagtttggat ccagaccatt ttctaacgct gccattgaag ttaccatctt
    27241 aaatgaagaa cctggctgaa attccccttg tgtaaccaag tttaaaagtg gacttcctgc
    27301 tagtaagtca tttggatttt ttggttgaag tgctttataa tcctctgtag aaataccttt
    27361 tgcaaattta tttgggtcat aattaggata acttgatgaa gctaatactt ctccattttt
    27421 aacatcaata gctataagag ctgctgactg tgcttttcct gcatatgcac taattggttt
    27481 gtccccaaat ttacttttaa aagtacctcc tttagaagca acttcgatta tctgttttaa
    27541 tgcattgtct gaaacttctt gtaaatcttt atctagtgta agatacacag tatctcctga
    27601 tttaggtttt ttagattcaa tttctttact gattctaccc aatgcatcaa ctttaaccat
    27661 tttataacca tcagttcctc ttaatctact ttcattactc ttttctactc ctgcaagacc
    27721 taccatatct ccagtttcat aacctttttg aaggtatgat tctatttgag tggatggcat
    27781 ttttcctaca tatcctaaca tatgagaagc taatgaccca ttaggataat atcttactgg
    27841 ttctactgct attgaaacac caacaaggtc catagcactt tcttcaattt gagcaatcgt
    27901 agtttctcct acatccttag caatagttac tggattatat tgagaatatc cttttgattt
    27961 tatcaaatcc cttacgacca ttatttttct tgcatcttca tcactcaaac ttttatcaat
    28021 ttctaatgca tcactatttc ttactttttc aaacgcttct ttggcactca gtttagtttc
    28081 ttttatctta taactagcca accaatctct cttatctctt tctgctgtga acatccattt
    28141 actaactaaa ataggtggat aatatccatt ttcatttagt tttgcctgta atcttgtcgc
    28201 atccaatctt ttatcttctt gagataatat tccagctgat ataagtttgt ccaccaagta
    28261 ataaaaactt tccttagcat tataatcatt tggaatacca ttttctgatt tatattccct
    28321 aatatctctg tcatatgtat aatagtactt tccattttct acatatatag gaaactcatc
    28381 tacatattct tcaccatttc tctccagcaa atttataagt tttaatgata tttcatttgc
    28441 tcttgaatgc ttatttgcat ctttcttatt aatatcgtta cctgaaactt gaacagtaaa
    28501 taagttttta tttcctgcca acagtcttcc atatctatct tttatttctc ctcttggagc
    28561 ttctattgcc aactttttat atgttttatt ctcagctaac tcattgtaat attcatattt
    28621 aaatgtagtc atatagatta tttttactaa aatagctaaa aatatcacgc ccatgactat
    28681 cttaattatt tttagtctat caacttgttt attttcttcc atgaaatcac caactaatct
    28741 tctttaagtt ttaaaactgc tcttttagat aagcgatata ttactaaacc aattaaacta
    28801 ttcactattg gtgctactag tagacctttt acaaaaagtg ttgatatgtt gtagctttga
    28861 taaattatac tagatgtacc tatattgaca attgaatcaa ttaaactgcc tataaaaatt
    28921 aaaacgaaag taataatata actaccttta tatagctgat ctctcatgta tccaattgca
    28981 tatgcaataa taaacaaaat taatgcattt acaccaaaaa ttccaccaac ggttatgtct
    29041 ttaataagcc ctattattgc tgctataatc ccaccttcta attcatctaa ataaagagag
    29101 attatggtta catatataag aactaaatta aagcttatgc caaaaatatc tatataattt
    29161 gttatactat tttctatagt cataagcaca atccccaaca gacaaagtaa aacttttttc
    29221 attgttttta tcccctttat cctatatttc ttggttctat gactacaacg tcatttatat
    29281 tcttaaaatc aacataaggt tttacaacta catattttaa tgacttatct ttatcatcaa
    29341 ccactttttc aacttcgcca attggtatac catctgggaa gaatccaaga cctgatgttg
    29401 ttattatatc tccttgaatt acattatatg atgagtcaaa catatatcct tgcaaatatc
    29461 ctttactatt ataactatct ttattatcta aagtagtatt ttgagtaatt gtacctttag
    29521 cattggcatc ctttgctagt ttaaagctta cagatgcctt agaatccaat agtgaaattg
    29581 ccttactata attgttggaa acttcataaa ctatgccaac taatccacta ccattcataa
    29641 ctatactttc tttttttaca cctgaatttt ttcctgcacc tatgacaaaa ctctcatacc
    29701 agtttccatc atttttacca acaacacttg ctgaaatact tgtcgcctta tactcttctt
    29761 gtacaaagtt cagtgtcttt tttaactctt ctagcgattc tgttttatct agtttggcat
    29821 ttaaagctat catatttttt ttaagttttt cattttcttc ttctaatttt tttacctttt
    29881 tagaattatc tttaaaattt gctacatctt taaaaccatt tttgataaaa gaaaatccat
    29941 catttattcc cttacctaca cttgtaatag catctgaagc tactcctaaa tttactggac
    30001 taccactaga aaatttacca attgatatac ccacaattcc aattaaagtg atagcaacta
    30061 cgcccgttgc tatcacttta acattaatcc tttttttctc atttttgttc ttgtcaaatc
    30121 tcaaggccat caccaacttc taattcttag tattcatcat taatactttt tcaaagattt
    30181 cttggtcttc aacagattta cctgttccaa gtgcaacaca atcaagaggt gtttctgcta
    30241 tttgtacaga cataccagtt tcctgtgtaa taagcttatc aagacctcta agcaaagcgc
    30301 ctcctcctgt tagcattatt ccgttttcca ttatatctga ggctaattct ggtggagttc
    30361 tttctagtgt agattttatt gcatctacta ttgaacttac aggttctttt aatgcttctc
    30421 taacttctgt agagcatatt tctattgttt taggtaatcc tgagattaaa tctctacctc
    30481 ttatttgcat atttatttct tcatcatctt taaatgtaga acctatgtta atttttacat
    30541 tttcagcagt tctttcacct atcataagat tatattcttt ttttacataa gccactatag
    30601 actcgtcaaa ttcgtctcca cctattctta tagatttagc agtaacgatt cctcctaaag
    30661 atattacagc tatttctgaa gtacctccac caatatcaac taccatatta ccttctggtt
    30721 cttcaacttt taatccagcc cctatagccg ctgccattgg ctcttctata aggaatgcat
    30781 ctttagctcc tgcttgtctt gcagcctctt ctatagctct cttttctact tctgtaactc
    30841 caaaaggaac acatatagct attcttggac ttacaacacc ttttttatca gccgcttttt
    30901 gtataaaata gctaatcatt gattgagtta tgtcaaaatc agctattact ccatctttca
    30961 taggtcttat agctactata tttcctggtg ttctacctat catttttttg gcttcttcac
    31021 ctactgctaa tacctcttta ctatcatctc ttattgcgac aactgatggt tctctaacaa
    31081 caataccttg tccttttatg taaactaatg tatttgcagt ccctaaatct attcccatat
    31141 cctttgtcat cttattaaaa ctaaaaaaac cttttttttc tttctttttt tccttagcca
    31201 tgattttact ccttcctact attaagaatc atgtttaaaa cattcttcta ttattttatc
    31261 acatataaag tcaaattatc atattttctt taaagctaaa gtataatcca tctccaataa
    31321 ttatatggtc tattatttct attccaatta tttctccaca tttaataagt cttgatgtga
    31381 tatttttatc ttctcttgat ggttctacac ttcctgaagg gtgattatgc atcactatta
    31441 tcttacttgc acttcttctt atagcttctt taaaaacctc tcttggatgg actaatgacg
    31501 agcttaaagt ccctatagat acatctacat cagatattat ctcatttttt gtgtttaaaa
    31561 gaaccgcttt aaaaacctct ttttttaaat accttaggct atccatataa tacctctgta
    31621 tatcccaagg attccttatt ttatacttta taggtttaaa actagctact ctagagccta
    31681 gttctaaagc tgctttaatt tgggttgatt ttgataaccc aattccatct atattacaca
    31741 gctcctctat tgtcatatct tctaaatgtc taataccctg aatatctcta tttattatat
    31801 agttggctaa ttcaatagca tttttattct tgttgccagt tcttaaaagt atagccaaca
    31861 actcagcatt tgataagctt tttacgcctt tggcaagcat cttttctctg ggtctttcct
    31921 ctggtgccat ttcttttacc ttaatggttg aattaaatga ctttttcaga taaatcacac
    31981 tccataaaaa agatttatac taaaatattt ttttaatagg tcgcctaacc ttgaaattgg
    32041 aagaccaact atattaaaat aatctcctct aatttcttca actaacaaag ctccataacc
    32101 ttgtatacca taggctcctg ctttgtcaaa tgactcatta gttttcagat aatcctcaat
    32161 atcctgctct gataacgttt taaatttcac attgctgatt acatagtcaa ttaccttttt
    32221 atcttcgcac aagtttatta agctaatacc tgttatgact tgatgctctc ttccagatag
    32281 tctttttaac atatctcttg cacaagactc atcttttggt tttcctaaaa tagcattgtc
    32341 taaaacaaca actgtatcag ctccaataac aacatcttca ttatgttcag atgctacaga
    32401 catacttttt tcaaaagcta atctcataac taaatgtttt ggtgtttcac cttccaatat
    32461 aatctcatca atttcatttt taattatatc aaatcttaca ttcgtattct ctagaatttc
    32521 tttccttctt ggagaggctg atgccaagat tatattcatt tattcacctc atttgtacgt
    32581 aatttgtacc tatttagtct atcactatat tgtaaaaaat tcaacagaaa tgtcgaacct
    32641 tttttgttac actattgtta tatttattac cattagaata aatatacata aaatagaggt
    32701 atggaaatta ttccatacct ctgttttatg tactcattag agatatggaa attattccat
    32761 atccttattt tgtccatatt acaaaatttt aatagctttc tttggacatt tttccataca
    32821 taaatggcat cctacacatt tatctgcatc taccttatgt ttttctttta attctccctc
    32881 tattgcatca aacttacatt gttttttgca cactgtacat ccaacacaaa gttcttggtc
    32941 aatagtaact ttctttttct tagttatgtc acctgatatt acttttgtag ggcattttgc
    33001 tacacattgc atacaaccta cacacttatt agggtctatc ttagctattt tgtcttcaaa
    33061 tattattgca tcaaatttac aagcttttac acacattcca cagccaatac atccagcagt
    33121 acatttttct ttaacagctt taccaaactc tttgctatta cactctacaa ctacttcttg
    33181 actttctggt tttgtgatta ttataccctt aggacaaact tctttacatt ttccacaatt
    33241 tacacacttt tcttcatcta ttactgctat tccatcaact atagaaatag catcaaattt
    33301 acaagcatct ttacatgttc caagacctaa acatccaaat ttacacatct tagcacctga
    33361 gtttaaaaca tttgctgctc tacaatctga tattccttca tactcatatt tatcttttgc
    33421 acttgaacaa gttcctttac aaataacttt tgctacttgt ttctcacctg cttcagcact
    33481 tattcccatt atttctccaa cttttgcacc aacatctgct ccacctacag ggcaaccatt
    33541 aactggtgca ttgccttcaa ctatcgcatt tgcaagacct ccacaacctg gaaatccaca
    33601 acctccacag ttggctcctg gaagtactcc tagtatagct tccactctct cgtctacttc
    33661 tacagcaaac tttttagatg caaaatctag tactatcccg aagattaagc ccataattcc
    33721 taaaactaat acagctgtaa gtatcaccat tttcacccct cttttttata gctttataag
    33781 tcctgtaaat cctaagaaag ctattgacat taaacctgct gatataagcg ttatagggaa
    33841 acctttaaat gcttctggta tatcagctaa ttctagtctt tctcttatac ctgcgaatat
    33901 tactattgct aaagtaaatc cagctccggc tccaaatccg tttattattg tttctactaa
    33961 attataactt ttttgaacat taactagagc tataccaaga actgcacagt ttgtagttat
    34021 aagaggtaaa taaactccta atgcttgata taaagatgga ctcatctttt gaattaccat
    34081 ttcaacgaac tgaactatag aagctataac caatataaaa gctattgttt gtaagaatcc
    34141 agttccagtt tttactaata aaatctgtat aaaataagtt ataattgaag ctagagttaa
    34201 aacgaacgta actgcaactc ccatccctac tgctgtatca accttcttag aaactcccaa
    34261 gaaaggacat atacccaaga attgagatgt tataacgtta ttaacaagaa cgatacttaa
    34321 aaataaaagt attaaattca tttattacac ctccactatg cttttttact tcttaattta
    34381 ttaaatcctg caaataagaa tcctaatgtt aagaatgcac ctggtggtaa tataaatatt
    34441 aatactggtt ggaaagatgc tccaaataat gtcattccaa ataaactacc atttccaagt
    34501 aattctctta ctgcacctat tattgtaagt gcaacagtaa atccaagacc ttgacctaca
    34561 ccatcaacta ttgaaggcat agctcctgtt ttgaaagcaa aactttctgc acgagctaat
    34621 attaaacagt taacaactat taatggtata tatataccaa gagccttatc aagagcagga
    34681 acatatgctt ttaaaagcat acctactatt gtaacaaatg tagctataac tactataaat
    34741 ataggtattc ttatcttatc tggtgtaatt tttcttatta aagatataac taaatttgca
    34801 catattaaaa ctgctgctgt tgaaagaccc ataccaattc catttattgc tgaagttgta
    34861 acggctagtg ttggacacat acctataact tgaacaaatg ttgggttctc atctattagc
    34921 ccatttttaa atactttagc taaattcata attaaccctc cttatttatt aagagcacta
    34981 ttgaaaacat ctatagcagc atttactcct gtagtaactg ctgtagatgt aatagttgca
    35041 cctgatattg ctaatatttg attatctgct gttgtacttc cttttgctac ttcaagaggc
    35101 tcagcagttt ttcctttgta ttgaccctta aatgcatcat ccttagcttt tgctccaaga
    35161 cctgctgttt ctgacatatt accaatgtca acacctgtaa cttgaccttc agatgatata
    35221 cctaccataa cttcaatttc tccaccatat ccacttggtt tagcttttaa agtatatcca
    35281 accacttcag aaccatctaa accttcatac acttcagcta taagaccatc tccaaggcct
    35341 ttgtaagtat cttccttcat ttctttaaat tcttttgctt ctggaaggac tgcttttcta
    35401 agttcattat ttgcttgtat atttctttgt tctattactg gagctgtaac ctgattagta
    35461 gctccaagag ctaatgcagc tattgcactt atagcaagta aagtaccacc taatctcacc
    35521 atactgttca ttatttagcc acctccccaa atactctatt tctaacaaac ttatctataa
    35581 gtggagcaag acagttcact aatagtattg agtaagatac accttctgga tatccaccat
    35641 acattcttat aacagttgtt ataagaccag ctaatacagc gtatattatt tgaccttttt
    35701 tagtcatagg cgaagttgta taatccgtaa gcatgaagaa tccacctaac ataagtcctc
    35761 cagcaaacag ttgatatatt gcgaaagtta tattaaatcc acctaaaagg aatgttaata
    35821 taaatacagt tgctatgtaa aatactggta taacataatt tattatgcct ttatacatta
    35881 ggtatactcc acctaataat aataatattg ctgatgtctc acctatacat ccaccaatat
    35941 tccctatcat catgtcagca agactaattc cattgctaga aaggtctgcc agtcctgctg
    36001 ctccattttt caaaaagctt aatggagttg ctgtagtaac tgcatctaaa tttttaccta
    36061 tccagttaac acctggttga gtccaagcag tcattgctac tgggaatgat gctaataaaa
    36121 atgctcttgc agcaagtgct ggattgacaa agttacatcc aattccacca aataccattt
    36181 taactactaa tattgcaaat attcctccta ctagacacat ccaccaagga agtgatgctg
    36241 gtacgttaaa tgctaataat aatcctgtta caacagctga aaaatctcct atagtacttt
    36301 tttgcttcgt aaatttttga tataaaaatt cagtacctac tgtaccaagt atacaaaaaa
    36361 acatagcact caaagcatta agtctaaaga agtatactcc tgctactgct gctggaagga
    36421 gtgctataat aacttgtttc attatatatg aagtatcttc attacttctc acatgaggag
    36481 aagatgatac tatcaactta ttttccatcg tttttccccc ttaactttct ttatttctga
    36541 gcagcttgct ttcttctctt tgctcctatt tctctctttg ctgatcttat agattgaagt
    36601 aatggtcttc ttgctggaca aataaatgag caagacccac attctataca atccattgct
    36661 ctatgatatt cagctgtgtc atagtcatct tttaaagaat atgcacttat gaaaagtggt
    36721 tgtaagaatg atggacatac atctgtacat cttccacatc ttaaacagtt ttgaatatct
    36781 ggagtacgtg attcttcctc atccaaacat aaaattcctg aagaaccttt gttagtcgct
    36841 atttcagtag tatattgagc tattcccatc ataggtccac ccattataac cttaccaatc
    36901 ttcttatctt ctttaaatcc accacattgt tctattattt ctgaaactaa tgttccaact
    36961 tttgttatta ggttctttgg ctctttgatg caactacctg ttattgtagt gattctttca
    37021 accaaaggca tacctgtttt aatagactta gctatctgtg ctgctgtggc aacgttgtca
    37081 acaactgcac ctgctgctat tggtaatcca ccagatggaa cttctttgcc tgtacacgca
    37141 taaataagtt gtttctctgc tccttgtgga tatttcactt gaaggccaac aacttcgatt
    37201 tcgctatagt ctttagctac attttgaatt gcttctatag catctggttt attcgtttca
    37261 attcctataa atcctttttt gacatctaat accttcatta atgctcttaa accaaacaca
    37321 acatcttctg gattttcaac cattaatctg tggtctgcag ttaagtatgg ttcacattct
    37381 gcaccattta atataactac ttctgccttg ctatctggtg gaggagatac ttttacatgg
    37441 ttagggaatg tagctccacc catacctact attcctgcct cttttatgat acctactata
    37501 tcttctttgc tcaaatcctc taactgacct tttggttgaa cactctcatg taattcttcc
    37561 ttaaagtcat tttcgataac tacacattga gctgaacctc ctggtacttc atgttgttca
    37621 atagcaataa ctttacctga tactgaagaa tgaacgttac atgaaacaaa accttgttgt
    37681 tcacctattt tttgcccaaa ctttacttca tcaccaactt caacaatagg tttagctggt
    37741 gcccctatat gttgctgaag aggaatgtaa actatctttg gagcttgagc tttttcaagt
    37801 gcctttgtgt tagagtactc ttttctatat ggaggatgta tccctccctt aaaagttaag
    37861 agtttcattg ttttaccccc ttatctataa aaaaacattt tttctgcctt ctataagtat
    37921 actatatata ttaaaaaatc ttaacaattg ttaaaaaaat atccgtattt ttataattta
    37981 ttttataaat caacacctct atattaagtc aaattgagga aaaggattgc ttaattaaac
    38041 aatccttttc atatagcttt ctaaagtcca aattttctgt attttatttt atagaattta
    38101 taaacataga gtactcctgc atggatgata aaagcagact tttacttaca ctatattttt
    38161 cttcatttgc tgcttttgaa gctgtatcaa ttttttcatt cacattcttt acatactcta
    38221 ttaaattatc tttaaataaa cgcatttcag aataatcaat cttttcagct tcttttgata
    38281 tattttgtga tattgccttt ctatttgtca aaatagtatt atatttttcc atatctacat
    38341 tattttcagc attcgaccaa aaacttgatt cttctttaaa gtttgttatt aatttattta
    38401 attctttaga tatactctct atataagcgt agttacttgt atactctaaa gacaacatag
    38461 gtgcatctaa gtgtgataaa aatgcatctg gaaacacctt tcttacactg cttatctgct
    38521 ttctaacatc attttcatca aaagaggaat aggtctgtat tttttttact ccatccttct
    38581 ccataactga aaaaggtatg tcattattaa ctaatgatgt ctgtattttt ttcaaatcat
    38641 cattattatc tattgcagca acttgaatag tatacatatc ccagccttct atagtagcta
    38701 atttgacttc ttctggctct ttagtgttat ttaccgcttt ttctggatta gtttcttgtt
    38761 tatcatctga attggtttgt gatttagatt ttttcccttt ttttattgaa tttaattcat
    38821 cagatatatc tttataagtt atatctgatg aattatttag aaatggtatt ttagccgata
    38881 catactccaa tatcttgcta tcttttattt ttgtataaga ataatagcca attagacaca
    38941 agcaagcaat cgttataaca tatttacgta aatcaatctt atgtctatta ttattaaatc
    39001 ctctgtatat cgtttttctt ctattcatta gtttgtccct cccaaaatta tattattaat
    39061 aaagtatatt tttaagagtc acaatttatg aatataaata attatttctt ctaatttttt
    39121 aataagaaat tgtacttact aaacaaatct tcttgcacca agtaatactt tagaccaata
    39181 tgaactagtt aaattatcca ctttaacctt attaggatta gtctgagtac cactacaatg
    39241 gataaattta ctatttccta cgtatatacc tacatgatta gttgttccat caccatcagt
    39301 gtcaaaataa actaaatctc ctggtgcata atttcccata gaaattgcac ttccaaaatt
    39361 agcctgttct ctagaaactc taggaataga aacgcctaca gaatgtttaa aaacatactg
    39421 tgtaaatcca gaacagtcaa atttatcagg tcctgaagct ccccattgat atggtattcc
    39481 aataagagta tatgcaaaat ctacgattgt tgaacctttt gctgtagatt tatctgaatt
    39541 tccagtaaat gatggctttg agttactatt agattgagaa ttttggttag atgatgaatt
    39601 tggtttttct tttgtatcct cattagtctc agaaatataa ctggcactca cccatccaat
    39661 tgttccactc gataacttta ccttatacca accattactt ttagctttta attcaacaac
    39721 atctccacca tttaattttc ctattataga atagcttgtc ccaggaccac ttcttacatt
    39781 taaactcaca gcagatgtta ccttaccatt cttgccttca attgattctt cgctagatgg
    39841 ttttttatca gaattattag tactatttga attactagaa ttgttcgttc caacatcttc
    39901 tgatgtttta gaaatatatt ggctactagc ccatccagta actccatttg atagttttat
    39961 tttataccat ccattgcttt gttctagtaa ctttactaca tctccattat ttgctttacc
    40021 aactaatgaa tagttagtac ctgcaccact tcttacattc agtctagaac tcacatttac
    40081 tttaccattt ccactaatag ttgtcccact tgaagttgga ttattttgac ttgatgtacc
    40141 ttcagacgaa ccacttgttg atatgtattt tgcacttccc catccaacaa cagaactcga
    40201 tacccttact ttataccatc cattagactt ttctaaaatc tctacagttt ttcccttgta
    40261 taattttgct accttatcgc aatcagaact tggtccactt cttatattca aggcacttgc
    40321 tgttactgtt cccttttcaa gagcacttgc atttatggag ctaacagata ctgctactgc
    40381 cccaattcct aaagctgcta tagctttttt cactactatc actcccccca acaaattttt
    40441 atagtcataa attcataaca ttattatcca tttttagtcc ctacaattta atgataactt
    40501 attttcttat taattgtaaa gaaattataa acatatcctt aaaattagtg atttttagga
    40561 agtatttcaa tccaataatt atctgggtcc gctataaaat aaattcccat actttcattt
    40621 tcaaaacata tgcaatctaa atctttatga agtttttttg cctcatcaaa ctcctcaact
    40681 tctaaagcca gatgaaattc attgtctcct aaattatatg gtctatccca atctctaagc
    40741 caagttaatt ctaatgtatg actagtaatg ccatctccta aataaactaa tataaaactt
    40801 ccatcttctg cttccttgcg ctttacctct ttaagcccta aagctttttt ataaaaatct
    40861 aaactttttt ctaaattagt aacattgaaa ttgttatgac aaaaattaaa cttcattttg
    40921 aaatcactct ccaattttta ttttttttat attatatcat tattataaat ttatttaaat
    40981 catattaagt taaaaatttg aatgatgatg accaattacc aaatcatggt aaattattta
    41041 aaagaatatg tcaaaaaaag aataagctct ttcaaatgca taaaaaattt atacccaagt
    41101 tatacttaat catgcaaatt ggcagaactt attctttaaa actctaactt ttaagttttt
    41161 ctttaataat ataatttatt actttttttc taatatcttc agcattataa tcatcttcta
    41221 cttctatatc caaaagtttt gctattgacc tcatatcttc tctagtcttt gacctatagc
    41281 ttgatattaa ttcatctttt attttctctg gttctaaaag atattcttca tctcctcttt
    41341 tgaaaacaac gtatttttga gcatatgaac ttctattgta gtatatatat cttgatattt
    41401 ttcttaaaat ttgattatat gaaagtcgct tgttaatatc aagttttaag tactctgaaa
    41461 aagcaaatag ctcttttttt gatttaaata atgcttcttt ctttatagtt cttgatattg
    41521 aagttacact ttcttgatat gcacactttt ttaacatttt taaatatact ctattaagac
    41581 caatactttg attgaatttt tttatattat tattaataat cttatccaag ttaccttcaa
    41641 agtatttttt tgctcctttt tgtgatacat aactctcaag ttctttgtgg gccatatcaa
    41701 gtatcatttt ttttaaatcc tctttatatc ccctagattc catgatactc cctcccttag
    41761 ctatttatag tatattcaaa aagctcaaga aatgtgagtc attacaaaat ttttttactt
    41821 tttatgtata tattaattta aaaggggaga taatagtaat gtaaaatcaa acatactcaa
    41881 tctcccccat ttttagacct ctattactcc cccaatagag gtcttttttt agtttatcaa
    41941 tttctattca attcccttta gttcaaatcc taattctgct atcacttctt ttaatttttc
    42001 aatagataca tcatctttca tatctacaga tgcacactta ttttctaaat ctacatctaa
    42061 aacttcaata ccatctatat cttctgttaa tgcagtttta agatggttta cacaatgacc
    42121 acaactcata ccttctacta atagtttctt cttcatttaa ttacctcctt ataccctata
    42181 agggtattct atcatatagt ttatactttt taaatagaaa ttaatcattt aatttgattc
    42241 attcgatttt atatctaaat tagatattcc ctcatataca tcaaaatcat ttggagtaat
    42301 tttacctttt aatctactat tacttacaat atagttgttt ctcacagcta aacttataaa
    42361 tgttgcatta tcttttatat atgtctgtac atctttgtat gctttttttg tttcaatttc
    42421 agacgtagaa cttgtaagtg cagacatata tccatttaac ttatcatcag tatatccact
    42481 actcgctata atactacttg catctggtat aattgatagt ttccatccaa ctaatgctaa
    42541 atcataattt ttagaattaa gagcattttc catatcctta tctgagagct gatttactgt
    42601 actatttatt cctatagctt ttaagttttc atttattatg tttgcagttt ttattctctc
    42661 actattatct ctgtttacta ttatctttaa gttcatttca gaaagacttt tctttacttc
    42721 ctctttttct ctattttttt tctcttcagc ttcttttttg attctatctt cttctgcttt
    42781 tttaactttt gcttcttctt ctggcgtaag cttctttgag ttctttttag ctgatttttt
    42841 ctgatttttt actttattgt cattttgatt tactgagttt acatttgcat attctacctt
    42901 agcaagacac tctatcgcct tatctttatt aaactctaaa tccttcattt cactattata
    42961 gtattttgat tttgaattaa gtggaaaatt aactggagta gcatcatcca tataaccttc
    43021 actaataatt ctatctttat ctatagagtg tgcaattgct tttctaaaat taacatcttt
    43081 gaaaaaagga ttattataat taaataatac acactcataa tctcttcctt gatattttgt
    43141 tatattaaac tctttttctt gaaacttact taaatcattt aatgtaacat tggttatatc
    43201 actatcaaga gccataacca tagatgtcct tgcatcttca ttaggcacca tacctacttt
    43261 tatgttcttc atggtttttg gaacttcttc atagtaatct ttgtttacag ataaaaccat
    43321 tccttctctt tctgtatatg attctatttt atatttccca tttcctatca tattattatt
    43381 cttgtcattt acatcttttg aatctaaatg attttgagat acaattggaa aaattaaact
    43441 atcaattgaa aaagcatatc tagctttaaa ttttattgta aaatctttat ctgatttaat
    43501 attcacagaa gctatatttt tagtaaattc attatatgga ctatctgcac ttttttgaat
    43561 taaaccaata gtatagttta catcatttga agtaacattt tttccatcat gccactttgc
    43621 atcttttagc tttatatcta tactcattcc atcctgagct atattatact ccttaactaa
    43681 ttgaggaatt acattgtagt tctcatctat agtaaataaa ctatcataaa ctaaattcat
    43741 tatataacca acagacttat cagtattagt tataggattt atagtttttg gagttaccat
    43801 tgttaagttt atatacttag catctataga actttgactt tgtaaacttg aatcactact
    43861 actttgtttt gttttagtat tactacaacc agctaccata agagttatag ctaaaacgac
    43921 tgttaagact tttattctct tcattagttt tttcctttct tgccatatat cttattataa
    43981 tgttcgtaat tattttttac tttttctaca tattttgaag tttgcttaaa aggtatatca
    44041 tgtagatttt cgccatcttt actatattca ttattttcta accatttttt cacattacct
    44101 gaaccaccat tatatgcagc tatcactaaa tctaatttac caaattcttt gtataactta
    44161 cttaaatacc aacagccaag tcttatatta gtctctgggt cgaaaatatc cacatttttt
    44221 aaatttagtt cctctgctcc ccaatctcta gttatgtcta atatttgcat taatcctttt
    44281 gcttcctttt ttgaaacagc agaactatta aacttacttt cggctttaat aacactgtaa
    44341 actatatttt catctaaatt aaattctttc gaatactttt ctacatactc tgaatatttt
    44401 ttaggatata aaaatttatg tattactttg ctttccatta atagtgcccc aaataagatt
    44461 ataaaaatag atagaatcaa tactttctta ctattcacgc caattctcct tcatgtactc
    44521 aataaattta tgtgctttac tttctaattc agttattgtt cccgaattat ctattatata
    44581 atcaccatat ttggattttt cttcctgact catctgtgaa tttatcctac taagtgcttc
    44641 ctgctcactg caattatctc tcttttgtat tctactaatt tgtacttctt gtttacaagt
    44701 aactactaat agcttgtcaa ccatatctaa aaaaccactt tctaccaata ttgccgcatc
    44761 taacactact atattttcgc ctttttttct caatttttct atttcactta ttattttttc
    44821 tcttattcgt ggatgagtca agttatttaa ttccttaagt ttttcttcat cactaaaaac
    44881 tattttccca agagcttttc tatttagagt tccatcatca ttttttatac tttgcccaaa
    44941 atggacaaat actttttcca ataacaactt atcctcaaat atcttcctgg atataatatc
    45001 agcatccact ataggaatgt taagatttct aaatatattt gataggctgc tttttccaca
    45061 gcctattcct cctgtaagtc ctaatatcaa cataaaatca cctactttgt ctcataccaa
    45121 gaactaccta catttaaatc tacatctaag tgaacatcca tatttacagc attttccatt
    45181 tcttctttta ctattttctt aactatatct atttcatcat caattgcttc aactatcaac
    45241 tcgtcgtgaa cttgtagtat aagttttgat ttcaaattgt tctcttccaa ttttttatga
    45301 acatttatca tagcaatttt tattatatct gctgcacttc cttgtattgg tgcattcatt
    45361 gcaaatctct ttcctctatt tctttccata aaattgcttg actttatttc tggtatatat
    45421 cttcttctgt ttaaaattgt tacagcatat ccatcctcac tagccttttc tactgttgtg
    45481 tccataaact cttttatttt atggtattta gcaaaataac tttcgatata ttctttcgcc
    45541 tttggaacag gtatatttaa atcatcagct agaccaaagt cacttttacc atatataata
    45601 ccaaagttta cagctttagc tgcacctctc aattcactag taacatcttc cataggaaca
    45661 ttaaaaactt gtgaagctgt tttggtatga atatcttcat gatgtttaaa tgcttctatc
    45721 atattttcat cctgactcat atgtgcaaga actcttagct ctacttgtga gtaatcggca
    45781 tcaaccaact tacagttatc atctgcaata aacacttttc ttatttttct acccatttcc
    45841 atttttatag gtatattttg aagatttggt tctgtagatg aaattcttcc tgtagtcgtt
    45901 atagtttgat taaatgatga atgtattcta ttactttctg gatttattat atttaaaagt
    45961 ccttcaacgt aagtagaatt taactttact atttgtctat actcagttat cttatctatt
    46021 attggatgct tatctcttaa tttttctaaa acatctgcat tagttgaata tccagtttta
    46081 gtttttttaa ttattggaag ctctaatttt tcaaataata taacacccaa ttgttttggt
    46141 gaatttatat tgaatggctc tcctgataat tcataaattt ccttttctaa tgtagctatt
    46201 atttctttaa attcaacact caattcatcc aatatacttt tgtctacttt tataccttca
    46261 tactccatat gacctaagac ttctactaaa ggcatctcta catgataaaa aagaccatcc
    46321 atactcatat ccattagact tctttccatc ataggaactg tttctttaac tgtgcatatt
    46381 atttgaccca tatactctgc taaatcctca aattctaaat cctcatagtt tttagattta
    46441 acaccctttc ctaataattc ttcttttgat ttaacctttt ttgataaata cttcatagct
    46501 attgcactac aatcatatga ggtagatgaa gttgaatcta ttaagtactc agctattgtt
    46561 atatcaaaat acatattttc taagtttata ttatatggtt ttaaagatat ataatcatct
    46621 tttaaattat atccatactt tttaatttca ttatttgaaa atatactatc taactttgac
    46681 acttcatttg aatctacata atatattttt tctccatcta cacttataaa aatatatatt
    46741 atattttttt caagtatatt accttgtcct ctaacagttt ttaaaataac ttgtcctttt
    46801 ttgtttactt catctataaa tttttctata tcttttaatt ttaatatttc tattttttct
    46861 tctacgtgtt ctgtgtcatc tgaatcgacc aaatcaacta atcttgataa gagacttgta
    46921 aatccaaagt atctaaattt atctaaaagt tcatctttat cataatcgcc aaaaatcata
    46981 ctatctaaat ccacttctat tggcacatct cttataattg ttgcaagtct tttactttga
    47041 atagctattt ctttgttttc ctctattttc ttttttacac tacctttaag cttatctgta
    47101 ttctctatta gattctctat actagaaaac tcttttataa gctttatacc tgttttttca
    47161 cctattccag gtactccagg tatattatca gacttgtctc ccataagacc ctttaaatcg
    47221 ataaattgtg ttggagtcat ctcgtatttt tcaattacag aattaaagtt gtattcttca
    47281 acttctccaa ctcctttttt tgttataaga gtagttgtat tgtctgaagc aagttgaata
    47341 gcatctttgt ctccagttac tatgtatacc ttatatccat tttcttctgc ttttttagaa
    47401 acagtaccta taatatcatc tgcctcatag ccatctattt ccaatctatt tattctaaat
    47461 ttatctaaaa ggtcttttaa tggctgtaat tgttctgcca gttcatctgg cattcccttt
    47521 cttccagcct tatactcttt atattctaaa tgtctaaatg taggtgcttt tctgtcaaat
    47581 gctacactta tatgtgttgg tttatatata tctatcattt taaatagcat tgttgtaaat
    47641 ccatatattg cattggtctt tagcccttct ttattattca tttctggaag agcataaaaa
    47701 gctctattta ttattgaatt tccatctatt ataattaatt ttttttccaa attatatcac
    47761 tcctaaaaat tatctatttt tgtaacatat atattatata ccaatatact gttatttaat
    47821 attcccataa taataatagt atattaaaat taaatatgtt gcaaacaata tatttttttg
    47881 ctataataat atacatgaca tgcccgagtg gtggaattgg tagacgcagt ggactcaaaa
    47941 tccaccggac taatactccg tgccggttcg agtccggcct cgggcaccat taaaaactcc
    48001 taaagctatt tattcaatag attaggagtt tttttatttt tgatttaaat tttaagttat
    48061 tttttgaaat tactgtgtct ttttaaaatt tatataatta tataaattta tcttctagca
    48121 ttaatttaaa aactaattta tttttatata attttctatt ttatatcata acttattaca
    48181 actttttatc taatttattg tatacaacaa cactacaaat aaattgaaat aaaataagca
    48241 atccaagaac aactaaaacc cacttgctta ttaccattac agaaaataca ctcaatatag
    48301 aaacaataaa tataagaatt acaagaacat aggacatgat ttgattagtg atacgtccag
    48361 ctttatcacg taacataatt cgtctttcat cattcatctc tatcctttga ttttttatct
    48421 tttcttcata atctactgct ctttcaggtt tactccaata catatattga agaatcatac
    48481 atacacctgg acctaaagca gctccagata atccccaaat aaaagcctca aatgaaaact
    48541 cagtaaatgt agccaataaa acaagtaaaa ttccacaaat aaaatatata attccaacat
    48601 ataacatatt ctttttcatt tttactcctc ctcataaata aatatttctt caatactcat
    48661 atcaaagtat ctagctatct taaaggctaa gatgatagaa ggattatacc taccattttc
    48721 taatgaacct atagtttgtc ttgatacttg caaaatagaa gcaagttctt cttgttttat
    48781 gcctctttct tttcttattt cttctaatct atttttcaca tctatccccc tttaatatct
    48841 caactacgga aagtctactt tccataatta tacaactata ttccatttat gtaaagctta
    48901 ctttccatat ttacaaaaaa ataacagact attttcagcc tgtaatttta gaatttcatg
    48961 ttgtttatta attttaatgt tatttattaa ttccatatta tttattaatt ttaacttggt
    49021 ttctgaattg gaatccaaac ttctgtagta taatcaggag cattgatatc gccattggga
    49081 taaagttcaa tatctggtaa attagcatac acatacccag aacttggtaa ccactcagta
    49141 ataattcttt tttgcaattc ctgtattgcc agagcatttg gcattggaga aacacattca
    49201 aatactgccc atgtgcctga tggtattaag tattcatgag tatcttctaa tacaggctta
    49261 tctgttggag cagcaatata ataatctaaa cccttactat ttgtacacac actaactcca
    49321 agcaatccac aaggttcatt atttattaac tctgaaagtt tatcaattgt tccatttttt
    49381 atgcatttag cccacaactt aggaactttc tcaaagcact cttctacaat catgttcata
    49441 ctttctttta taccaacaat acgaaaccct ttcttttcta caatacgata ttgcatttct
    49501 atatttcctt ttacataaat gcttaatgtc atcttaggat atgcttttaa aacaacaccc
    49561 tttgctctag ctgcagatgg agaaatgtta tgtatatttt gaaaagctct attaaaagat
    49621 gttggtgaat cgtaaccata cttctcagat agttctaaaa ttttgatgtt actgttctga
    49681 agttcaaatg cagccttagt catacgtctt cgtctaatat attcagataa tggtacttct
    49741 gcaatatatg aaaacattct ttgaaaatga aaggttgaac aacatgcaat ttttgctgct
    49801 tctgcataat ccactccact ttcgagattt tcttcaatat aagtaatagc ttgatttaat
    49861 ttatttatcc attccatttg atattacctc caagcaaatt atacagttta ataaaataat
    49921 atacctctct attcatgcac aaaattgtaa gcatttaaat ttataagcat ttttcaatca
    49981 atacaataaa tataaaaatt gtattataca ataaaaaagt tcaataacat tatattataa
    50041 aatgttattg aactttttat aaaaattaat ttaagtgtag ttaaattaag tattacacta
    50101 tcttatatat tacaatatcc tactatctta tagtcttctg gcactttata atctttgtct
    50161 ggagtaccta aattccattt taaatcaaac atagttgcac ttattattgt tgcaaaataa
    50221 agcataacta taccaatgtc tattttttct tcatataaat tagtatgacc atcttttcta
    50281 acagctaaaa ctacagtgcc tccatcaact ataaatctcc atggttgtct atttaatgta
    50341 gatggagcta atcttgcata gtgaaatgca tcaagaagtg ctctttcttc taagatttct
    50401 atacttgcaa catttcccca ttcgttcata taaacaacat cttctacacc tagtctagta
    50461 gaccccttat ctgttacaac ttgcatatca gattttgaat aattttgccc agtttttgta
    50521 gcatttaata ctttagcatt acttacattt tctccatatc caatagctat aattcctgtt
    50581 acctctttat ctgaattaat tccaagtttt tcttttacct tttttccatc ttcaaaagta
    50641 acccaacaag aaccaattcc aagttctgtt actttaaaga ttatactttc tcctatgtac
    50701 cctgaatttt caatgtaatt atcttttatg tcagataata ttataatata acttggagaa
    50761 tctatcatat ttcctttgta ccctgctact ttatctaaat tattaaaaac ttcacttcta
    50821 ttcataattc ttacatcaac atctatttct ggaactagtt tcttacaaga ttctgagtat
    50881 ttcttaattt cactaagata ttcttcttta atatctgtgt tcttatattc tctaacagac
    50941 tttctattta atatgattgt tttataatcc ataattttat cctccccaaa ctagtattgt
    51001 cttaattata tacccattaa tatattattt tactcattta tttaataaat aaagattaca
    51061 attttgtaat tattatttat taataatatt ccacttttac attaataatg accagttgtg
    51121 caaaatccat tgccaaccac caaataaaaa catattataa aggtaatata aaataacaaa
    51181 caaatcttaa caaaaaatta aacttttttt acacgaaaga aggcttcccg ttgccttctt
    51241 tatttttata aaaaaataag ctgtttctaa aatgatttca catttttgaa acagccaagt
    51301 tattctataa tgacaactat aatttgagga tgtgagggag agttgtcatt ataaatttag
    51361 ggtaattata ttatgtttta agctagttct ttctatattt atataataca ttttatatat
    51421 gaattttata tgagattggt ataaatgata tatgaaatta catatgttta tattatatta
    51481 aattttatta ttacattttc cacaacaata ctcatatgtc tccacattat ttggaatatt
    51541 tacaaataca aattctttac agttctcaca atactttaat atagatatag ttccacaagt
    51601 gtaacattca aatattacac aatcctcatt ttcctctact atttcaaact ttttactatg
    51661 ttcaccacac tctgggcact tgtgattatt tacattcata aatactcctc cttattagta
    51721 tagttacatg tcaaaaactt aattaatgta ttctatatta cttattatat attctataat
    51781 aaatcatatt ctaatataat aaaacaaatt aaataaaaaa aaaccataaa ttttaaattt
    51841 atggattaat tcttgtaatg ataactctat ttgaggatgt gagggagggt tatcattaca
    51901 atagggtatt atattatggt ttttaagcta gtctttctac acctcatatg atacatatgc
    51961 tatatgaatt ttatatgatt tttacataaa tattttatga aatttaaatt ttttagtcat
    52021 tttgaaattt gtaaaaagtt ctctaaaaat ccacagcatc ataaacagcc aaaatttaag
    52081 tagaacatca taaaaaaatt tgtccaataa ttttaacaag taatctcacc tcattataat
    52141 tattgacaca aacttaaata aacaatctat aaatcttcta taaatcttaa actttccccc
    52201 tattgataca caatttctag tttgtcaatt ttattttgca tttctaagaa aatttcttca
    52261 acagaattgt taaatatatt gcctaaatag attcttttat ctttttgaaa tgaataagat
    52321 tttacactac catcagaaga aatatataaa tgactccact ctgctccata ttttgaaaac
    52381 ctctcctttg tatctttaaa cctcatatct tctattttta ttgctttttc tctgtctcct
    52441 cttactacag tgtgacccct ttttattaaa ctggttattt ttatatcttc ttttaattta
    52501 atacgtgtat catcattttt tgaatatact ttccatatca ctttatgacg ataattatct
    52561 aattccttta ttaagcttct atcaatagta aaactatctt tagttaccac aattaaatca
    52621 aaatggtcta acatatattg aaatatttct ctcatacttt tattttttaa aatatcctca
    52681 ccaataacct ctataaatct acagcctttc tcatacatgc agttcaataa acttgtgatt
    52741 tcagaaaaat aatcatttgt attattatct ccatctattt ctatagtaaa gtacaaagga
    52801 gtaatgtatg atttatttcc tgttatctca aatacacttg atagtgtctg tatatcatct
    52861 tcaaacgtta agtatacatt ctcataaaac ataaaaaatt ccataacttt ttctttcata
    52921 ttatggatat ttctatatct attggataat atatttatta tatcgtcagt agtatttttc
    52981 ccatcacatt cagccagtat aacaatttgc ttccaactta agctatccac atttccgata
    53041 ctttttttat atccatcttc ttcatcataa ataggaccaa ttataatgtt ttcttttggc
    53101 cacagatgaa ccctagttcc atcttttaat ttcggatatt tattttttac agactcataa
    53161 aatctacttg tatcacccat gattctttac taccccccat agtttaaata ttctatgcta
    53221 aaaatagcac tttattttta ttaattcaac aattattcaa aattggtcat tatatatgtt
    53281 taaatggatt taacatcaac cttatttata tgttattgat gtttatatat gttatataaa
    53341 atcttatttc tcatacttca atgtaatctt aactatctct ggtttacaat taagcctaat
    53401 taaaactttt gagcctccta agcctttatt taaaatcata gtgctattat ctttttcata
    53461 ttttccccag tcatacttag gaaagaactc tctatttgga gataatacac ctccaacaaa
    53521 aggtattctt attatacctc catgaacatg tcctgctaaa accaaatctg ctccccattt
    53581 tgcatatttt tcaaagaaaa atggtgtatg tgctaaaaga atgttatatt cattttcatt
    53641 aactttacct agattgtctt ctacaaaatt gtcacttagt tctaattttt tgctattagc
    53701 aaataaatat ggatagcatt ccaatggtat aattaaacca tataaattaa gatggctatt
    53761 acctctatat attcttacag attcattttc catatttatt attttctttg aatatagttc
    53821 cttaaaataa tccttatata gatgtttata cttttttatt aaagattttt gttcatgatt
    53881 tccaattata tgatatactt catatttttc aaccaaatta tctattaaat ctaatgcaac
    53941 ttgaaaattt ttattttctc catctactaa atctcctgtt ataaatataa catctgggtt
    54001 taatgattca attttatcta tcaatacctt attattttta tcaaacacct tattatgtaa
    54061 atcactcact tgaactattt taaagttatc aaattccttt ggaatttttt tgttgaaaat
    54121 agtatattct gtcgtttcta tatgctgagt ttcatactta tacagaatat acaataatat
    54181 taatactaat actgtcattg aaacctttat aatcatattc tcttcctctt tattctattt
    54241 tgttaatatt attataatgc ttttatgttt tttttaaaac aataacatca tcacatacat
    54301 ataaatcttt tcgacattat aaaaataagt tatccttttc taaaattttc atttatttta
    54361 gaaaaggata actataaaat tatctataaa cctctatatt gtgaaatata gtttttcctt
    54421 tatttaaatt tttatatata tgctctaaag ttccatcaaa cctaatagaa tgtccagact
    54481 ctaaaatata agttttccca tccacattaa ggtcaagttt accttctatt acgagtacat
    54541 actcttctaa aacatgactg tgtgtagatg aaacatgagt acagttttcc tcaagctcaa
    54601 ttataaaaag ttcaaacctt ctttgagggc taaaagggta tattggatat aacttcatgt
    54661 tccctttttg ctcaacaata ggctctactt cttctttctt tataacctca taggtatttg
    54721 tattttcatt taatagtgta gtaaaattta ttttaaatcc agatgaaatt ttccataaag
    54781 tagatactgt cggacttgat tcacctctct caatctgtcc aagcattgct ttactgacac
    54841 ctgtcatctt tgaagctttg tctaaactta tgcccatttc ttgtctcaat aaccttaaat
    54901 tttctcccac attaggaaat ttattatcca tgttaaaatc ctcctgatag ctatttgtat
    54961 tctatgattt ttactcctgt aagttctact atctctttta attttaacat atgattgtta
    55021 caaatttcat caaattcata tatataatct tcaagcccta atgccttata tttatcgatt
    55081 ccaaatttat gatatggaag taactccatc ttgcctcctg gaacattttg tttgacaaat
    55141 aatgcagtat ttcttatatt ttcttcacta tcatttactc cataaataat tggcactctt
    55201 ataaccatag atttattcaa ctttgaaagc ctacatatat tatctaatat aattttatta
    55261 cttactcctg tatattcttt atgaatatta tcatccatag attttatatc tacaaatatg
    55321 tggtctattt tctcaaatac atcatttacc ttattccaat caaaatatcc acaagtttca
    55381 attgcaatat ttatgccttt atcataaaaa atatcaacta attctcttaa aaaatcaatc
    55441 tgaagtgtag gttcacctcc agaaaaagtt atccctccat tagattcaaa gaaaaatata
    55501 aaatcttttt caacttcctc tactatttct tcaacactca tttcttcggt cattatacaa
    55561 atggcatctt taagacatac cttgatacac tctccacata aatcacattt attatttatt
    55621 tgcgctctat caaatagtga acttatattt tgaggacaaa catctataca tctattacag
    55681 gatacacatt tatctttcat aacacctaat tttactatgt tactccagga atctggatta
    55741 gaacaccact tacattttaa tttacatcct tctaaaaata tagtagtcct tattccatct
    55801 ccatcattta ctgaaaaatg ttgtactttt acaactctac cttttttact cataatctac
    55861 ctcataatta aaaattgcta tgtgaatttc tagctattat agcatcttgc atttctcttg
    55921 ataaattaac aaattgagta ctatatccag caactctcac taataaatct ttatattctt
    55981 ctggattttc ttgagctttt aatagagtat catttgatat cgtattaaat tgtacatgga
    56041 atcctcctaa agcaaaatat gatttaatca tagatgttag atttctaagt cctctttcag
    56101 tttccaccaa atcttgattc agtcttaaat ttagaagtgt tccaccagta tgaacatcat
    56161 gatttatttt tgatgcagat ttcattgctg ctagtggact tgttgtatca gtaccaacaa
    56221 atggagaaac tccctctgtc aaaggttttg tcgcttttct tccacaagga gttgcgccta
    56281 cgatttttcc agttggtaca tagtttgata tacccataaa tgcactatta aatttagagc
    56341 caaaaatatc cttgtatttt ctagtttcag tataataaaa atcagaaact tcaattgcta
    56401 atgaatctac ataatcatta tcatttccat acttaggaac atccaacgca agttttctta
    56461 attcttcata accttcccaa tcattattta gtgcttttgt taattcttct aacgtagtca
    56521 ccttatcctc aaatactaat ttttttatag ctgccaatga atttgaaact acacctaacc
    56581 ctataccagt taatacagga cctatattat attttgctcc tcctttactt aaatcaactc
    56641 ccttatccaa acatccatct acacaagtag ataaaaatgg tcttggtacc atttctttat
    56701 gaatttgttg agcaacagta gttcctataa cagaactttt tacaagatat gctaattgct
    56761 ttaaaaactc atccttaagt tcttcaaaag aagtaaattc tgtgatattt tttgtatcta
    56821 accccatttt ttctccagtt aatctactct taccttcatt catagcatat tctaaagctg
    56881 ctgcaaaatt gatatttacg gctgaagtcc actgtcctgt ttttctaaaa tgaggaacta
    56941 cacaaccaca gttactccaa tctctagcat cttctgcatc atatccatct tgtaataaca
    57001 tttgtgcccc tgcactatcg ctgtgtatag cagggaaacc agttccttgt tttaccaatt
    57061 tagcaactgc cattacgaaa ttgtctggtg ctccttgact cactctaaca cttaatcctg
    57121 gttggtgagt ttttacactt tctgtagcct ttaaacacat atatgagata tcattcgttc
    57181 catcagtacc atttctcttt ttaccaccaa cagtaagatt ttggaattga ttatatcctg
    57241 caaagtaatt tgctgtattt gctgatatag tccataccca ttctgataac ttaatccaaa
    57301 gtgcttctat caattcttga gcttctgatt cagttatttt accttctcta gcatcatttt
    57361 cataatatgg atacatatat tgatcaaatc tacctaaatt taatgccaat ggattttctg
    57421 atagaatacc acctaattga acaaaccata caaattgtat tgcttcataa aaattagttg
    57481 gagggtttgc tggtacattc atacatatct ctgaaatttt tataagttct aactttctct
    57541 tttcatctgt ttcttgctta ctcatctctt ttgcaagatt tgaatatctt tgtgccaatg
    57601 tcataatacc ctgtgcaact atagaaacag atttgtaaaa atctatcttt tcaatatttt
    57661 ctgaaactga tatatctaat tctttaattt ttttatctgc atcttcctta atccctttat
    57721 atccttttac aaacaatata tcttgataat ctggtgttac ttcaccaact gcctgtctcc
    57781 acttagaatc attatctatt attcctgtat ctactaatat cttagctgta tcttctggga
    57841 ttcttgctaa gaaaacttct tccaaagatt ttcccttcca atatggaaat atttcttttc
    57901 ttataaattc tatttgttct tttgatatta catatgggtc ttgagttctt ttatcaaaag
    57961 tatccatttc tttgtctacc cattgccaag aaaactctgg tgtcaatatc cctgttcttc
    58021 taaattttcc tgctgtacct actattaatt cattttcaaa gatttttata ggaagttctt
    58081 tacatgtctc caaaaaagct ttagctcttc ttatacagat agcttctccc tcagtttttt
    58141 tatgtgattc tgtaaaaatt ctagcccttt cacaacatat cataggttta gaattaatat
    58201 attcttgtct aaatgcttct attctattag tttgactcat ttaaaatccc cctttggata
    58261 atatatacta catttttgta tgttataaca tactttaatt atattatact gtcctttagc
    58321 ataaaatgga agcaaaataa cttatttttt ttcatctacc ttgaacacct atatattaat
    58381 actttttttg ttaaatttat aaaatctaaa aaaattttct atgatatatt ttctatattg
    58441 ttatagttat gtctaatata ctaatgattt gaaatatcaa atttgtatac tagacttagt
    58501 tttaaaacct tagttgattg tttaactttg ttaacttcaa catgtatatc cttcatataa
    58561 ggaagctcta caacacctac tcccttatta aagtcttctt caaccataga aaaaatttct
    58621 ttctcagtta agttcgtatc agtcaatgat ttaaataaat tgtatataaa tttaacatat
    58681 ttatcactct cagataactt atcatcttct ggatactctt tagcacagag aatatctatt
    58741 gttacttttc cttctttatc gtcttcagac atagaataga ataaacttcg aatatttttt
    58801 tcaggtggtt tttcagcacc atttatagat actacaaagc taccatctga ttttgagtta
    58861 gtaatactat aaccactgtc tattatagct ttcgaaaatc tttgagataa atcttttttt
    58921 acttcttctc tacctttcgc atcattaatc ttttgtgagt aattattaaa tagctcaata
    58981 gattcatctt ttttatcttc aacttgttct ttcaatgaat ctttactaat ttgttctgag
    59041 ctacttaagt tcttatcaga tacaggagac atacacccta ccatcaatgc taagctaaga
    59101 tttaaagcca caattattgc cactctattt atattccttt ttactttcat acacaatcct
    59161 cccatcacac taaccgctct aaagtattat tcattgcact agaatatatt tatattttct
    59221 ttatattaaa gatttattat tcaatacata tcaccttcct atccaattct tataacttac
    59281 tattagtaaa attataagct ccttaataca gaaataagga tgttatcttt tgttattaaa
    59341 agataacatc cttatttttt tgtatcaata catatgtccc aactggtaac ttaatcgccc
    59401 caactgtcag tatcttggtc caatggtata tatatattta gtataaattt atcgatacta
    59461 ttttcaaata ataattctcc attatatttt tgtaaagaag atttaatact ctgaatacct
    59521 attccatgta cgaacttatc cattttgttt gttaacaata tatttttatt aaaagtaagc
    59581 tgatttagtt tactattttc acatttaagt acaaaaaatg accttgttat agttccttta
    59641 attctaatat atttattaat attttcatcc actattttat cacatgcttc tattgcatta
    59701 tccaaaatat tagaaaatat gcttgacaca tctataggtt ttacaaaatt tacttttgaa
    59761 aaatttatag agcaagtaaa tttaattcct ttttttaaac atacttcact cttttcgttg
    59821 attataatgt ctaatatcat atttcctgta ttatagatat atttaaatgt ttttatttca
    59881 tcttttaaat tgttaacata ttcattaatt tctttgctat tgtttctaag gttgtctata
    59941 caataaatgt gattatttat atcgtgatat agtttcttaa ctcgcatatg agattcttga
    60001 atacttaaat aatgcgcata ttgcatatct aacttatttt ttattaattc agcttgagat
    60061 tttaatctat tctcttttat tatttcttta aacaatagta taagaaatat actactagct
    60121 cctaccaatt ttggattaac aaaattatca aaatagtatc cttctgaatg taatgaccca
    60181 aaccgttcta atttttttaa tacataattt acaagcatat tcattgtatt aactaataag
    60241 gcaaataata aatatacata atttctctta tcttggctaa ttaattttat agactttcta
    60301 aaaaagctaa ata
//
LOCUS       DAMJZE010000004        56452 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJZE010000004 DAMJZE010000000
VERSION     DAMJZE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.004"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(96..1697)
                     /locus_tag="PC541_000176"
     CDS             complement(96..1697)
                     /locus_tag="PC541_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2837664.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1808..2485)
                     /locus_tag="PC541_000177"
     CDS             complement(1808..2485)
                     /locus_tag="PC541_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837665.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2498..3193)
                     /locus_tag="PC541_000178"
     CDS             complement(2498..3193)
                     /locus_tag="PC541_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2837666.1"
                     /translation="MDYLKLANHPLLWIVSTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3245..4444)
                     /locus_tag="PC541_000179"
     CDS             complement(3245..4444)
                     /locus_tag="PC541_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2837667.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4705..6750)
                     /locus_tag="PC541_000180"
     CDS             complement(4705..6750)
                     /locus_tag="PC541_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2837668.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7161..7970)
                     /locus_tag="PC541_000181"
     CDS             complement(7161..7970)
                     /locus_tag="PC541_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2837669.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     QKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8054..8587)
                     /locus_tag="PC541_000182"
     CDS             complement(8054..8587)
                     /locus_tag="PC541_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2837670.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8737..10086)
                     /locus_tag="PC541_000183"
     CDS             complement(8737..10086)
                     /locus_tag="PC541_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2837671.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10098..11480)
                     /locus_tag="PC541_000184"
     CDS             complement(10098..11480)
                     /locus_tag="PC541_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2837672.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11724..12938
                     /locus_tag="PC541_000185"
     CDS             11724..12938
                     /locus_tag="PC541_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2837673.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13115..15028
                     /locus_tag="PC541_000186"
     CDS             13115..15028
                     /locus_tag="PC541_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2837674.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15299..16273
                     /locus_tag="PC541_000187"
     CDS             15299..16273
                     /locus_tag="PC541_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2837675.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16294..16569
                     /locus_tag="PC541_000188"
     CDS             16294..16569
                     /locus_tag="PC541_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2837676.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16582..17745
                     /locus_tag="PC541_000189"
     CDS             16582..17745
                     /locus_tag="PC541_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2837677.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18082..20361
                     /locus_tag="PC541_000190"
     CDS             18082..20361
                     /locus_tag="PC541_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2837678.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20456..21796)
                     /locus_tag="PC541_000191"
     CDS             complement(20456..21796)
                     /locus_tag="PC541_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2837679.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21808..22590)
                     /locus_tag="PC541_000192"
     CDS             complement(21808..22590)
                     /locus_tag="PC541_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2837680.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22605..23627)
                     /locus_tag="PC541_000193"
     CDS             complement(22605..23627)
                     /locus_tag="PC541_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2837681.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23633..24646)
                     /locus_tag="PC541_000194"
     CDS             complement(23633..24646)
                     /locus_tag="PC541_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2837682.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24774..25712)
                     /locus_tag="PC541_000195"
     CDS             complement(24774..25712)
                     /locus_tag="PC541_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2837683.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            25982..26446
                     /locus_tag="PC541_000196"
     CDS             25982..26446
                     /locus_tag="PC541_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837684.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26558..26875)
                     /locus_tag="PC541_000197"
     CDS             complement(26558..26875)
                     /locus_tag="PC541_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2837685.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26888..27619)
                     /gene="chbG"
                     /locus_tag="PC541_000198"
     CDS             complement(26888..27619)
                     /gene="chbG"
                     /locus_tag="PC541_000198"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2837686.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27645..28955)
                     /locus_tag="PC541_000199"
     CDS             complement(27645..28955)
                     /locus_tag="PC541_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2837687.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29052..30365)
                     /locus_tag="PC541_000200"
     CDS             complement(29052..30365)
                     /locus_tag="PC541_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2837688.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30387..30698)
                     /locus_tag="PC541_000201"
     CDS             complement(30387..30698)
                     /locus_tag="PC541_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2837689.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30898..31602)
                     /locus_tag="PC541_000202"
     CDS             complement(30898..31602)
                     /locus_tag="PC541_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2837690.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31693..33054)
                     /locus_tag="PC541_000203"
     CDS             complement(31693..33054)
                     /locus_tag="PC541_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2837691.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33553..34707
                     /locus_tag="PC541_000204"
     CDS             33553..34707
                     /locus_tag="PC541_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2837692.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(34789..36507)
                     /locus_tag="PC541_000205"
     CDS             complement(34789..36507)
                     /locus_tag="PC541_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2837693.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(36512..37501)
                     /locus_tag="PC541_000206"
     CDS             complement(36512..37501)
                     /locus_tag="PC541_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2837694.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(37597..38646)
                     /locus_tag="PC541_000207"
     CDS             complement(37597..38646)
                     /locus_tag="PC541_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2837695.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(38923..39591)
                     /locus_tag="PC541_000208"
     CDS             complement(38923..39591)
                     /locus_tag="PC541_000208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2837696.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(39597..40970)
                     /locus_tag="PC541_000209"
     CDS             complement(39597..40970)
                     /locus_tag="PC541_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2837697.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(40967..42745)
                     /locus_tag="PC541_000210"
     CDS             complement(40967..42745)
                     /locus_tag="PC541_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2837698.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(42862..43185)
                     /locus_tag="PC541_000211"
     CDS             complement(42862..43185)
                     /locus_tag="PC541_000211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2837699.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(43172..44149)
                     /locus_tag="PC541_000212"
     CDS             complement(43172..44149)
                     /locus_tag="PC541_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2837700.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(44164..44727)
                     /locus_tag="PC541_000213"
     CDS             complement(44164..44727)
                     /locus_tag="PC541_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2837701.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(44779..45270)
                     /locus_tag="PC541_000214"
     CDS             complement(44779..45270)
                     /locus_tag="PC541_000214"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2837702.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(45273..47198)
                     /locus_tag="PC541_000215"
     CDS             complement(45273..47198)
                     /locus_tag="PC541_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2837703.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(47200..47511)
                     /locus_tag="PC541_000216"
     CDS             complement(47200..47511)
                     /locus_tag="PC541_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837704.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(48141..48416)
                     /locus_tag="PC541_000217"
     CDS             complement(48141..48416)
                     /locus_tag="PC541_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2837705.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(48531..49940)
                     /locus_tag="PC541_000218"
     CDS             complement(48531..49940)
                     /locus_tag="PC541_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2837706.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(50311..50973)
                     /locus_tag="PC541_000219"
     CDS             complement(50311..50973)
                     /locus_tag="PC541_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2837707.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(51227..53425)
                     /locus_tag="PC541_000220"
     CDS             complement(51227..53425)
                     /locus_tag="PC541_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2837708.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(53701..56355)
                     /gene="adhE"
                     /locus_tag="PC541_000221"
                     /gene_synonym="adhC"
     CDS             complement(53701..56355)
                     /gene="adhE"
                     /locus_tag="PC541_000221"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2837709.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        18739 a         9880 c         6788 g        21045 t
ORIGIN      
        1 aaacgaagca tgatttttat ttaaataaat ctcaaacttc gtctttactt acttagatat
       61 ttatttactt tctaaatcct attattttta aaaccttatc tttttaatac ttcaccacat
      121 ttattctcag taatctctcc attttctagc gcaatttttc catttacaat tacatattca
      181 acaccattgg ctagtaattg tggctttgaa aaatcaatat cttctgacct aggataacta
      241 tagttatcca tgtcaataac tgctatatca gctatcaacc cttctttaag aatacctcta
      301 ttttttattt taaattgcct agctggtaat gaagtaactc ttcttacaat ctcttccaat
      361 ctaacaccag actttctgta catattgaag aaaattggaa aagctcctct tctttgtagt
      421 tcaaaccatg acatatcccc acccacacta aacaaacaat ctgaaccaac cataacaaat
      481 ggattatcaa ttattttatc atcatgtagt ttatctggga aatcagctct atacattcct
      541 cctaaacaaa agataagttc ttcgtctcca tctaaaagta aatcgagtaa taacttgtcc
      601 tcatctattc ctttttgtgt agctatttca cgtattgatt tcttttccat attaatatct
      661 ttagtgttta gaagtattat actcccccta tctccaagta taaatgcttc ttttaatata
      721 agtgttcttc cctcttcatt tctaagagca tccattaccc catctatacc taattcaaat
      781 agactagatg atattgccat aataaattga attactcttt ttttcttcat acaatgccca
      841 gaacttcttg gtactgtgtc aaccattatt tcaacaccat tatacctagc ctttaatatc
      901 tcatcaaatg cttctatttc aactggactt aagtgagata cttgaaccct agcaccactt
      961 ttctgcccaa cctctatggc ttcttttact gcattatatc ttcctatgta atctctaata
     1021 tgagatgtat atataccatc atgctcttta atctgccttg caatatcaac aagttcatca
     1081 atatcagcat acttacttgg tatatatgcc aatccagtag atattccaaa agcaccactt
     1141 ttcattccat catttattat atctgtaatt tcattttttt ccttttctgt aggaggtcta
     1201 tccttagaac ctcccataac actccatctt atagtcccat gccccaacaa gaatcccata
     1261 ttgacagaaa taccactttt agatataagg tcacaatatt ctgtaaaatt attccaacat
     1321 ttatttttat ctataagata ctttttttct tcttcaagta atagaccatt tttatacata
     1381 tattcatata cattttcaga agaatatggt gttatagaat gtccacaatt accatttata
     1441 gttgttgtaa ctccttgttt tagaaacttt tcaaatttac catctagtat tgctactatt
     1501 tcttcatgaa catgagggtc aataaatcca ggtgttataa tttttccata acaatcaatc
     1561 tctttatttg tattgctatt tatcttagaa tctatttttg ctattaagtt gtctttaatt
     1621 gctatatctc cataaaaacc aggattacct gtaccatcta ttataaatcc attttttaag
     1681 attaaatcat atatcaaaat attccctcct taaaaaccaa tttttaaata atcttaatgt
     1741 aatttttata tttgaaaatc aaattattaa atttcatatt tgaaaattca ctattatttc
     1801 attgtaatta gtcactaagc ttatctttat acatagctct tgcaattaat atagtaccaa
     1861 atacacatat aagaactact agagactgtg gtattatacc ttgctttgat aatataatag
     1921 tcaatacaga tattacaatt gctactagac ctaatttttt atcttgtaaa aatacttgtc
     1981 caaaaactga cccaaataga gctggtaaaa ttaagtttaa tttttctact acttcagctg
     2041 gaattttaga tagtacgctt cctcctaaaa ttacccctat agttagtata cttatattca
     2101 ctaaaataga tacagctatt cctattgtag atattatcga gccttcttct gtacctgatt
     2161 caacttctgc tgccttttga gctgctattg aacatggtag cctcatatta gatatattac
     2221 cagaaagaaa actcatataa gttcctggga tacctaagat tggtgaataa gaaactggtt
     2281 ctattatgta atttggagca ctcatactga caattgccat tgtacttgct aataatgctg
     2341 atattggagg cataatacca aatccaaagc tcaaaattat tccaggaact aatgctgcta
     2401 taatacccaa agatagtgtt agtcttccat accttatcat aaaaggtaaa tattcttcat
     2461 tgtatataga ttttacttta ctcataaaac tcccccctta tattactgaa gctattatca
     2521 ttccgctaaa cattgatatt gtaagccccc actctcttaa ccattttata tttttcttct
     2581 tttcaagcat acacaatatt accattatag ccattccact tatacatgca attgttccat
     2641 tatcaaaacg caacacccta tcagcattta gatatgcaaa tgaacccaac attgctccta
     2701 gagatattat tggtataaat gatttcttgc cactagatat aacattggtt atcttatcta
     2761 gtttatgtcc aaatagtaat gtgaatatta tccatcctaa agaacctaat atcatagtcc
     2821 aaactgcatt tgtaaatgca agttttgtca tttcaggaga acctaaagtt atacctaatg
     2881 catctgttcc aaatccggct gacattgatt caaaaattac agaacctata aatgataatc
     2941 tcatccatgc tataggccca cccatagtta ctataagcga taccattcca gcaaatatag
     3001 tcattgaagg cccaatagct gaaatagcac tactcttaac agctgacttt atttgtttat
     3061 ctgttattcc cattgtttta ccaactttgc aagattttat agcaaatata agggattgta
     3121 ccactactat tgatactgct actgtactca ctatccacaa aagtggatga ttagctaatt
     3181 ttaaataatc catttataaa acctccctat aaatcctcaa attttattaa tatttctaat
     3241 atatctattt ccaaacctgt tttagtactc taagtatatt tccacctagt accttctcta
     3301 tttcctcatc actatatcca tgctttacta aatatctaac aatgtttttt gaagtttctg
     3361 ttgggttctc aagacctttt acatattcta ctttgggatg ttcttttgtt cctgttattt
     3421 tttttatatc aaatttagca gctagtactg agtgtaattt tacatggtcg ccatatactg
     3481 tatctggtcc aaaagctaca tggtctattc ctactaaatt tttaatatac tcaaaatgtt
     3541 ccataaatga ctctattgaa tgttctttat tcttctctgt tattgtagta tgaggtgctg
     3601 cttctacacc tataacccct ccttttttag cacatgctat taatacttca tctggagtta
     3661 gtctctttgt gttccataaa cttcttgccc caacatggct taatattatt gggtccttgc
     3721 tatattctat tacatcaagt gtagtttgaa ctccaacgtg agagcaatct attgccattc
     3781 caattttatt cattctttca acagcttttt ttccgaaatt tgtaagacct ccatctttct
     3841 cttcttttag tcctgaacct aatgcattgg attcgctgta tgttataccc attaacctta
     3901 taccaaatcc atacagtata tctattctat caagttcatt ttctattggt gctgcacctt
     3961 ctaatgttgg tatcaatgct actttacctt cacttttagc tctgtatata tcttctactt
     4021 tttcacactt tataacaaaa tcttgatgag ctaaatcaca tagtctcatg cctaaatcta
     4081 ttaatatatc atcccatttc caccctgaat ttgatgttat cgtacataca ccatccatta
     4141 aattatcaaa tatagcatca taatatcctc ttgataatgc ttcataagca catctttgtt
     4201 taccttccct attatattca aatatatctc tttctaggtg ttctggaaat aatactggat
     4261 gttcatgtaa tgatataaat attttttcat ccactatact tttaacctta gcttcttgaa
     4321 tatcatctaa ttcaataagg tattctttaa ctcttttatc tcctttacac atctcaatag
     4381 atgaaatatc ttttcctttt tctaaataac tataggcttc atatcctttg taagctgact
     4441 tcataacaac atcctccaag ttctacaatt ttttatattt ttcctatgta gcaattgatg
     4501 tgccaaaata taaattttaa ttgtataata aatcttgcaa aaaataaaaa cagtagtaag
     4561 gttgatattt actactgttt tattaaaaat aatcttctta gattatttat tcaatatttt
     4621 tgaattatct taatcttaaa gataatttta tttatttcta tccattatag ggattttttt
     4681 agggtgtttt gcctttttag tcccctatat cactccctta atttttacat atgatataaa
     4741 gttctttcct tcatcagtta taaaactacc ttgttttttt actccagata aaagtaaacc
     4801 atggctcttt aacttatcta ttctttttct aatttggtct acacttaaaa ctatatcttt
     4861 ttcttttaaa atctcttgca gtttatttct tcctaataat atatttttat tattccaagt
     4921 ttctatactg gtcaatatac atatactttc ctcaaagaaa ttagattgtt tgaaatctaa
     4981 gataattgaa tcgaaatttt cattaactaa agtattgttt ttttcaaatc taaattgttc
     5041 tggcaaatgc tcgtaatcta ctctatcttc ctcaactata gtatcaatat aataaattaa
     5101 atttttaagc tctcttacat ttcctggcca ttcatacaat gttaaagctt gcattgattc
     5161 ctctgtaaaa catttgttgc tatttatttc atctaaaaaa tacttactta taagaggtat
     5221 atcttcctta cgttctctaa gtctaggtat ctctatatgt aatacattaa tcctataata
     5281 caaatcttct ctaaaagaac cttctttaat ttttttcttt aaatctttat ttgttgcagc
     5341 tataattcta acatctattg gtataatttt acttccgccc attctcataa cttctttttc
     5401 ctgtaatact cttagcaatc tctgctgtac atctaaagat atatcaccta tctcatctaa
     5461 aaaaattgtt cctgtatgtg ctctttcaaa tatacccatt tttccacctt ttattgctcc
     5521 tgtaaaactt ccttcatcat agccaaaaag ttcactttca ataagtgtat ctgacaaaga
     5581 agatagatta actgcaacaa atggcttgtt ttttcttaga gattcattat gtattgcctg
     5641 ggcaaatatt tcttttcctg ttccattttc tccaagtatc aacacagaaa agtctgtagt
     5701 tgctattttt cttgcaatat taattttttc ttttattatt ttactctcac ctacaacact
     5761 ctcaaatgta tactttgata caaaaccttt tgactgaagt ttattttgaa tcttgtcctc
     5821 aagcacttga attgcactta tatttttaat gcttataata ctctttattc gttcattatt
     5881 ttcataaaca tttactttat taattatcag ttttttgtta tttaaattta ctacttcatc
     5941 attaacttca tcttcttgaa aaaatatttt tttgacaacc ttatcactaa ataagtccat
     6001 aaagttatta gatataattt cattttggtc tattccaact agattagaaa atactttgtt
     6061 gcaatatata aattttccta atttatcaac agaagcaata ccttcatcta taatctccaa
     6121 aaaactcttc atggttttat tcatagttgt ataataccta tatccactta ctgtatcttc
     6181 attgtatttt tccttaataa tatgtagttt atctatagat atatttagtt ttgtaaagat
     6241 ttctataact gtatttatat caattacctt atcacctatg tcaattattt gctttatgtt
     6301 ttgtggtatg ctgtttctgc ttcccgtaat aattccaatc tcacatttag acttatcaca
     6361 acctggataa tatggtatta gattaatatg aattaatcct agttttctaa ttatcttggc
     6421 agtttcatct gctgactctt tataagcatc tataaccata acatcagaat cattttctat
     6481 acttattatc tgatttatat tttcaatatt tatagtacga tgtactatta caataggtat
     6541 atttttatca atattattat gtatatattc ctttatatca ttgtctgaac atactacaag
     6601 ctcatagctt gacagttcaa tatttatcct atttacatat accaagttat ctatattaca
     6661 aaattctcca aatatatttt ccaattgttt tgatatatct atatttattt tgtcatcagt
     6721 tgatacaaat aaaatattat gttttttcat agtcccacct cctgtttaac gagtatagaa
     6781 agtaataatt tggtctattg tagcactttt taaaaaagtt ttaaatctta aaaatcaaat
     6841 tttgtaccat ttactattat tttccataat aaaataatgt aagctatctt ttttcacata
     6901 taatttaatt tctaaaacgg ttagtttgac attattggaa tataaataaa aatttaatat
     6961 aaattcgtta atatattaat acatttttaa gaatgacaag gaataaatta taacaaattt
     7021 taggaatata taataatagg gtctcagaac ttagttacaa gttctaaaac cctatttgtt
     7081 tagtgtaaaa aacatcataa aatttagctt aaatcttttc acataatatt actctttttc
     7141 taagtgataa taaaatatat ctattcctct aaaaactgta aaaattcctg attaaatagt
     7201 ttaagctcat catagaatat agcatgacca ctatactcaa aagtatataa aattgaatct
     7261 tctatttttt cattcataaa aactgcaaat tcatacggac atatttcatc taatttccca
     7321 tgaaatatac cagtaggcac tcttacacat tttaaatcat taaataattc ttcatctctt
     7381 aaagatacag cagttccaat agtgccaata cctgatgcac taaaaccaat atcattaaac
     7441 catcttctaa agctttctgg tggatttgat gcaaatactt tttcgccaaa atcggttacc
     7501 atttctggtc tatcagtaca tgcttgtgct atcaatttat ttacatcttc acgtgtcata
     7561 ccatatggaa attctggagg tctttgtaca aatgatggtg cagcagctgc agctaatacc
     7621 aattttgaca ctccatatcc attaaataaa ctcatatacc taagtacgat tgctcctccc
     7681 attgaaaaac caacaagggt aaagtctttt aatccaatag catgtaccac cttataaata
     7741 tcgtctgcta attggctata agtatatcct cctgatgttg catcagattt accaaaacct
     7801 cttaaatcaa ttgaaactgt tctatatccg agtttttgca atatatttgt ttgatattca
     7861 aatatcttat gtcctaaggg ccaaccatgt ataaaaagta cagttttttt agcactcgga
     7921 ttcaaatcat atactgctat gtttatatca ttcacatata cataatacat atcaacagca
     7981 actcccttca agaaaatgta tgatggttat tttatatata tgaataaaat gatgaataac
     8041 taaattttca aatttattta caatacttct ctattgcctt ttcaaataaa cttacatgaa
     8101 gtttttcatc taggattatt ctatttaata tagcttttat ataagggtct tcaataagac
     8161 ttatatcttt ataataatta ttaatagcca catgctccaa atgcaaatct agttttaatc
     8221 tgttacaaat agaatttcca taacatacaa aaccaccatt ccaatatgca ccacaagaac
     8281 ttgtagaacc tctatataca ggatttccac ccaataatct tatagtcttt gctaatatct
     8341 ccatatgtaa catttctgtt atagatatat ttatccacat ttcacttaac tctctatgcg
     8401 tttcatctaa atcaaaatta tggtatagat attgatttac actcgtaaat tcacttgata
     8461 ctccagcata gtcatccatc aataattctg cataatactt atttggacct aaaactttta
     8521 tctctggata tggctcatca cttgaatatc catttcgctt gtgcatgtca taatgtgcac
     8581 cactcataat atctcctcct ttaaatttaa tgtatgatat taattgtatg catcacaact
     8641 ttttcatata acttagataa taaaatctac ttaaataata aaaagccata ttaattaaaa
     8701 ttaacatgac cttttatcca caaaattact ttttaattat tcatcttttc cagctactga
     8761 tagctcttta ataacaactg aaggtgaacc tatactactc attggaaatt ctaaatcttc
     8821 tcctatagct tcaatatctt tcaacaggtc aaaatagttt cctgctactg taatttgctc
     8881 cactggaaat actttttttc cttctttgat ataaaagccc ttggcagcta aagaaaagtc
     8941 tcctgttact gaatttgcac ctgaatgaag tcctgcaaag ctagtaacca tcaatccttc
     9001 atctatatcc tttataattt catctaaaga tttatctcct ttctctatat aaaaattagt
     9061 tggatatatg cttataggtg atgaatatga agattttatt ccatttcctg tagtctttac
     9121 acctgcctta ttagccgttt ttaagttatg aagtaaagtt ataagctttc catttaaaac
     9181 aacttctttt ttaaatgtag ctactccttc atcatcaaaa ggagtagatg ccataccatt
     9241 ttctaaaagt gggtcatcaa ctattgtaac tataggagat gctatcatgt ctccttctct
     9301 atttttcaat aaagataatc ctttttgagc tgaatctgca ttaaatatcc cagaaaatgt
     9361 gctaagcaaa gataccatag cttcattaag caatatagtt ttatatttac ctgatgtaat
     9421 actcttacca cctactttag ataaagcatt atcaacacca agttttgcta tctcacaagg
     9481 ttttatttct tcaatagaat ttgctatatt atatcctatt ccatcatatt tttgtccatt
     9541 gtcttctatg ataggaacta caaaccctat aagcatatta gttttattgc ttaaattaag
     9601 acctttagta ttagatatac cattacttga tacagaataa gatatagtac atttagataa
     9661 atttaccacc ttatctgaat aagctttagt ctctctttcc atttctaaag ctaaatcaat
     9721 caacttatct gcctctaaat tttctagctc tttagaataa gtctttacat cattatagtg
     9781 cttatctcct tcatatataa attgtacatc ttcattttca atagtgttta ctccatcttt
     9841 tacatttttt atcaacatat caacagcttt atcatctaaa atttctgtat aagaataacc
     9901 tatctttcca tttaccttac ctctaaaaga taaaccaaaa gatttatcaa gattgtattt
     9961 ctcaacttct ccttcatata tatttatact taaattttct cctgtataat aatatatttc
    10021 acactcttca aaaccttcac ttagagcctt tttaaataat atatctttaa aatttttaag
    10081 ttccacaatt atccctccta tcttccacct acagtaattt cttttaccct tatcataggt
    10141 tggcctacat tagttggtat acttcctgat gatgagccac acatcccctg agcttgtttt
    10201 acgttattcc caaccatatc tatattcata agtatatcgc taccttttcc aattaaacta
    10261 gcacctctaa ctggctcttg tatttctcca ttttttatta agtaaccttc tgaaactgca
    10321 aaattaaact ctcctgttac aggatttaca gaaccaccac ccattttttt agcataaagt
    10381 ccatctggta ttgactttat aatatcttct ggtctatctt ctccagcagc aatataagtg
    10441 ttagtcatcc ttgaagttgg tgcaaatttg tagttttgtc tacgagaact tcctgtagga
    10501 ttcattccca ttcgtctacc attaagttta tctatcatat aagacttcaa aattccactt
    10561 tcaatcagta tattcttttg agttggattt ccctcatcat ctatattcat agaaccccag
    10621 tagtttggta ttgtaccatc atctatagct gtaatctttg tggaagcaat ctgttgacct
    10681 aatttatctg caaatacaga gttccccttt gcaactgatg tagcttccaa agaatgtcca
    10741 caagcttcat gaaatattac tccaccaaat ccattatcta ttgctaccat catattacct
    10801 gcaggacaat ttttagcatg caacatagta tgcgctactc tagctgattc ttttgcataa
    10861 tattctgggt ctatttcttc aaatatttca aaccctttac aacctccagg accttcaaat
    10921 ccagtttgat tttcattacc cttagatgca attgaactta tccctagtct agttcttatt
    10981 cttctatcct caacatatat accttcagtg tttgcaatta atattttttg gtctttatcc
    11041 aaataactga cacttacttg agatatatca ttactaaatt cttttgcact cttatatgca
    11101 accttcataa cacttacttt atctttattt cctattgaat ttggataaat ttttatatta
    11161 tgtatgttat ttattttaat actgtcattt aatattacac tttttccatc ttccaattta
    11221 cctaaagcaa cagcagcttt gtatgctaca tccaacagac tatttaatga attgtcactt
    11281 gtataagcat atatactttt caatccttta aatattctta ttcctatacc ataatttctt
    11341 ccacctattg cattttctac tttattatct attaaactaa tagaattatt tatagtgtct
    11401 tcttcaaaaa tctcagcaaa atctcctcca gtaattagac atttttctaa taccttagaa
    11461 gctatatttt ttgaaagcat aaatttacct cctactttaa atttttaatt aacttttatt
    11521 caatcttttt acttgttaac aaacattata tactatatta cttaaaataa ggtaattatg
    11581 aatatattat acatatgttt tatactaaaa tgttatcaca ttttatctat acatacccat
    11641 ataactagat aattaaaatg tataatatta tttgctatag aaaataatac actcatacat
    11701 aataaatttt aggagtgaag tatatgaatc aagaatatgt aaaaggaaat cctattttta
    11761 ctaatattaa taaagttcca agacaatttc cataccttac tgatgatatt gatactgatg
    11821 ttataattgt tggtggtggc gtaactggat gtatttgtgc ttactatcta gctaaaaaca
    11881 atataaagtc tgtcattctt gaaaaaggaa ggattgcaca tggaagcact agcgtgacaa
    11941 cttctctatt acagtatgaa ttagatgaca atttgataga tttaacagag gttatgactc
    12001 taaaagatgc tctaaaagca tacaaccttt gtacttcagc acttgaagaa ttagatacat
    12061 ttatagagct atatggaaat aagtgtgatt atgccaaaag agatactcta ctttatactg
    12121 ccaacaaact tgaagttaag gctatcaaag aagaatataa cttaagaaaa gaaaatggat
    12181 ttgatgttga atatattgat gaatccacaa acccttttag ctttgattta aaatcaggat
    12241 tgatagcaaa gaatggagga cgagaattag acccatataa atatagtcac catttgattg
    12301 atgtaagttt aaaaaatggt cttcaagtat atgaaaatac agaagtaaaa aaagtagatt
    12361 tatcaaatga taaggttact gctgaagtat cgtatggata caaagttcat ggtaaaaaat
    12421 taattgtagc tacagggtac aatacaagtt tatttacaaa aagaaatttt gcaactaagt
    12481 caaatacttt taatatagca acgaaaccac ttaaaaacat agcttcttgg aaaaacaaca
    12541 ttttaattag agataactgt gacccatata attatctaag aactactaaa gacaatcgat
    12601 taattatagg tggtgaagat gtaagttttg atgatataga aaatgaaact ttagccaatg
    12661 aaaagtatga tattttagaa caaagattga aaagtatgtt taaggatata aaagacattg
    12721 aagttgaata taaatattgt ggttgttttg cttcaacttt agataattta ggttttattg
    12781 gtccagataa taaaaataac aatctatggt attgcttagg ttatggagca aatggtatac
    12841 tctttgcaat attaggaggt ataatgttaa gtgaattata tcttggcaaa caaaataaaa
    12901 atatgaaatt atttaaagta gatagatttg ataaatagat attatatatt aaaagactgt
    12961 aaaaagatga aactttataa ttctaaaagt taactttgat acagtctttt ataatgttcc
    13021 ttttaaaaat actttattta taattaaata taaagtaaaa tgttaaaata taactaataa
    13081 aatttagtca taagataata tttaagttta tttgatgaaa tatggaggaa ttattatgaa
    13141 ggaaatactc gttattgctc ccactaaagg aacctatgaa aaatcaattc acatagttaa
    13201 aaaaaacaaa tatacaaata ttgatgttgt atttggcaat ctaaaagaag gtatacctct
    13261 tgctgaaaaa agtattaatc atggtactag aattatcata agccgaggtg gaacttacaa
    13321 tatgttaaaa gctacttaca atatacctat agttgaaatt aaagtagatg cttatgatat
    13381 tataaaaagc tacaaggagg taaaaaattc caacgaacca tttggaataa ttggatttaa
    13441 taatgtcatc tacggtttcg atattataga agaaatattg aataagaaaa taactatgat
    13501 tgaaattgaa aaagaagaag aaatttatga tgctatagaa aagtacagaa agaaaggtat
    13561 aaacacatat ataggagata ccactgttgc acatattgtt aagagactta attgtaaggg
    13621 aatattaatc gaatctagag aagaaaacat acttagagcc attcaacaag cagaacaaat
    13681 actcgaagca accaaggatg aacaaaaaag aagattacag atagaagcta tgacagattt
    13741 tgtacatgat ggaataataa cagttgataa aaattttaaa ataacattat ttaataagag
    13801 tgctgaaaaa atatttggta taaaaaaaga aaatgcactt tatcataatg ttgtagatgt
    13861 tatcccaaac acagttcttc cacaagtagt tgagactatg gaacctcaaa ttggtgaaat
    13921 acaaaatata gggagtacaa aaattattac aaatagagtt ccaataattg ttgatggtca
    13981 gattcaaggg gctgtttcaa catttcaaga tagcaaggag ataagttctt ttgaacatac
    14041 aataagaagg agcttattaa gcaaaggtct atctgctaaa tacacttttg atgatataat
    14101 tcatgctagt gaaaagacaa aagattgtat agaacttgca aaaaaatatg cttgttatga
    14161 tactcctatg attattactg gagaatctgg agttggtaaa gaactatttt gtcaaagtat
    14221 acataactat agtaagcgta aaaatgcacc ttttgttgct gtaaattgtg ctgccatacc
    14281 tccatctcta atagagagtg agttttttgg ttatgaggaa ggttcattta caggtgctag
    14341 aaaaaaaggt aaagcaggtg tattcgaact agcacatgaa ggcacaatct tcctagatga
    14401 aataagtgaa atccctcttg aacttcaagg tcgattattg agggtattgc aggaaaaaca
    14461 agttatgaga attggtggtg ataaagttat tcctatagat gtaaaaatta tctgtgcttc
    14521 aaataaagac ttaaaaaata tgatgcgcaa tggtttattt agacaagacc tttattttag
    14581 aataaatata ctaacattat atcttccttc attaaaagaa agaaaagaag atattataaa
    14641 attatcagaa ttttttatac ataaatattc aagcaaatac aataaaacgc ctttaattat
    14701 cactccaaat attaaaaagg cacttcttga acgagaatat gaaggtaata taagggaatt
    14761 ggaaggtatg atggaaaggg ctgttattgt ggaatcattt gactcgattt tattagaaaa
    14821 gcttagcaat gaatatacaa ataatatagt tgataaagat aatagtgatt ttgaatgcag
    14881 taatttagat ttaaaaactt tagaaaaaaa atatatctat aaaatttata acgaaaataa
    14941 tagaaatact acaaaaacct gtgaaattct aaagataact cgctccactc tatggcgtaa
    15001 gttaaaagaa atagaagatg atgtttaaaa ataatacata tctatatata tcaaaacgtt
    15061 ttatgtttaa ttttaataca taagacgttt atttgttttc aataaaaaat acaattatat
    15121 gctaaaaaga atctttgtaa gtgaaattct cgtcaatcat acttttaatt ataagtaatc
    15181 tacagcattt caatcgatta ttaaaaataa tcatgataac taaagataca aactctatat
    15241 attggcatag taattgcttt tataatacct atataaaaat gaaagggagt gatactagat
    15301 gcacatctta aaatcagtga aaaagatacc aggaggactt atggtctgtc ccttgttatt
    15361 gggagcacta tttaatacag tatgtccaca agctcttgaa attggaggat tcacaacatc
    15421 cttatttaaa actggtgcaa tgagtatact tgctctattt tgtctatgca atggagctca
    15481 aatcaatatc agacaagctg gtaaaccatt gactaaaggt atagtactta cagcaacaaa
    15541 atttataatt ggtgcagtat taggtattat tgttagtaaa tttattggac ctaaaggtat
    15601 aattggtatt acacctcttg ccatagttgc taccatgact aattcaaatg gaggtctatt
    15661 tgctgctctt gcaggtgaat atggagactc cacagatgtt ggagcaatat ctatcttatc
    15721 tctaaatgat ggtccttttt ttacaatgct tgcttttgga gtaactggac ttgcaaatgt
    15781 acctatagta gcactatttg cagcacttat accaatacta cttggattta tacttggtaa
    15841 ccttgatgaa gatatacgag aatttcttgc acctggtaca acattattaa ttcctttctt
    15901 cgcttttcct ttaggagcag cattaaactt taatcaaatt ataactgctg gactacctgg
    15961 agttgtactt ggtttaggag ttactataat aacaggtatg ggtgggtact ttgtaatgaa
    16021 acttatacgt gcagaacatc cagcagtagg tgcagctaca ggtacaacag ccggaaatgc
    16081 agctggtact cctgaagctt tagcagctat agaccctact ctagctacaa tagcagctgt
    16141 ttcaacagta caagttgctg ctgctattat tgttacagca atatgttgtc caatgttagt
    16201 ttcctatcta gataaaagag aaaaaaagaa aaaatctatt tttaaaacaa ataaaaatat
    16261 tgaagtaaat taatacttaa ggaggtaaaa ttaatgaatg caattattca tgcaaatgaa
    16321 aaagataatc tagttacatg tgtcagacct ttaattaaag gtgaaaaagt ttcaattgat
    16381 ggaaagtctt acatagtaaa tgataatata cctgtctttc ataagatggc tacagaagat
    16441 gtaaaaaaag gtgatgttgt atacaaatac ggtgaaatta taggaattgc aacagttgac
    16501 ataaaaactg gtgactatgt tcatgtacat aacattgaaa gtacacgtgg tcgtggagat
    16561 aaaaaatagg aggaagttaa aatgaaatta atgggttatt taagagaaaa tggtcagttt
    16621 ggtattcgta atcatgtttt aattattcca acatctgtat gttcaagtga gacagctaca
    16681 agaatagcat cacttgtgcc tggagcaata gcaatccctc atcaacatgg atgttgtcaa
    16741 attggttctg atatagagct tacagcaaag acattaatag gatttggaaa aaatcctaat
    16801 gttgccgcag ttttagtagt tggtcttgga tgtgatggta ttcaagcaaa agaattagca
    16861 tctgaaatag ctacaacagg aaaaaaggta aattacgttg ttatacaaga atgtggtgga
    16921 acattaaaaa cagtttctaa gggagctgaa atagtaagta aaatggctcg tgaagtatca
    16981 aaagaagtaa gagttgaatt tggtatgagt gaaattacat tagcacttga atgtggtggt
    17041 tctgacccaa caagtggaat agcttcaaac ccttcaatag gcgttgcatc aaaccttctt
    17101 gtagatgaag gtggaagtag catactaagt gaaactacag aagtaattgg tgcagaacat
    17161 ttgttggcta cccgttttga agatgaagaa atgaaagata agtttcttaa atttgtaagt
    17221 gatgtagaaa aaagagcaat tgctatggga gaagatttac gtagtggtca acctactcca
    17281 ggcaataaag ctggtggact ttcaacaatt gaagaaaagt cacttgggtg tatgtataag
    17341 gcaggcaata agccattcaa aggtgctctt gaatatgctg atattgtacc tcctgataaa
    17401 aaaggattat attttatgga tactccaggt caagatatag attctatcac tggtatggta
    17461 gcaggtggtg cacaaattgt aatcttttca actggtagag gtactccaac aggaagccct
    17521 atttcaccag ttattaaaat cactgggaat agtgatacat acaacaaaat gccagacaat
    17581 atcgatataa atgcaggtag aattataaca gatggtgcta aaatagcaga tataggtcaa
    17641 gaaatattta atgaaataat agaagtctgt aatggtaaac ataccaaagc tgaaagtctt
    17701 ggtcatagag aatttggcat ttacagaatt tcatctacat tttagattgt aacaaaatat
    17761 cttttataaa tataatactt atgtagatac ataagctaaa agttctttct aatactataa
    17821 aaattttcta tactgaaaag gaactgtatc aaaattattt tgaaaacagt tcctttttta
    17881 acctctttta ctcaaaatat tataaaataa gatttaaaca ataaacgtat cttgattaat
    17941 ctctaagaag acataaataa agctaaatgt aaagtgggat aaataaaatt taatatcttt
    18001 gtatgaaaag atatttggta taatatgtct gagagaattc ttatttgtaa acaaataaag
    18061 gatggaggat gaaacaaaag aatgaacttt gaaaacacaa aaaataaaaa aataatagct
    18121 actttactat tgacactcct tttaatcgtg tcattaacat gtgtcacttt tattatgaat
    18181 ctaaataaag taacacataa tgaaacagaa cgatatcttg tagaagtttc acaaagtatt
    18241 tccactacaa ttaatgtgaa atgtaaaagt atatttagga cactatattc tatgggagat
    18301 acttattttg agattaatga tagtgaaaaa gaagtcaata gatacttatc aaaaaaatta
    18361 aaattatttg aatttgattg gttaggtatt atagattcta atggaaattt tgtaagtgcc
    18421 cttaaagata caggtaatgt taaaggacaa gacttttata gaaaagccct tcaaggcaaa
    18481 gaaacaatta ctcctgttaa atcacaatct ggtacatatg gaattttata ttctgtacca
    18541 ctatacaatg gtgataaaat tgttggagct gttgctgctt ggaatactat tgatactatg
    18601 agaaatttat taagtgtaga aagctttcat ggtgaaggat tttcccaaat tatcgaccaa
    18661 gatggaaatt ttattgtaag ttcagcaaac aaaaatgctc caaaaaatgt ggtaaatttt
    18721 tttgacatga ttaaacacca aggaaaacag gttgataaaa ataaacttga agaaatgaaa
    18781 ataaacttaa aaaagaacaa aactggttct atgtattata cattaaatga tggtatttct
    18841 aaggcaatgc attatcttcc attacaagaa gaaaacttat acctcctttc aattgttcca
    18901 acaaaaacag caagtgcaca gtttgattta cttctaaaaa aagcaatctt catcaatatt
    18961 ttaattgtta tactattttt attgcttatt atattgatta tgtttataaa tcaaaagaac
    19021 agggcacaac ttataaaaat cgcctatgta gactctgtaa ctaaaggtta cagtaaagca
    19081 cgttttgata ttgaagctaa aaaaattata cagtcatctt catcagaaag ctattcattg
    19141 gtatctataa atattcagaa attcaaactt attaatgatt cttttggtag tgaagcagga
    19201 gataaaacat taaagtacat acatgatgtc attagtaatt atctagaacc aggagaattg
    19261 ctttgtcgag tttctaatga ccagtttaat gtacttatta aaactattcc tcaaaaagaa
    19321 attcttaagt acatagaaaa tatcaccaat gatattaata gtttcaataa aaatgaagaa
    19381 tgtaaatatt ttatagctct atctataggt gtatataaaa ttgatgaccc aaatctacct
    19441 ttagtttcaa ttcaagaccg tgcaaatgtt gcacgaaaaa ataataaagg aaacattgga
    19501 aatcatccac tttatacatg tgttttttat agtgatttag agcgtttaaa aatgattaaa
    19561 gaaaaagaaa tggaaaatag aatggaggaa gcattaaata ataacgaatt tatagtatat
    19621 cttcagccta aaataagcct caaagataat actattgtag gtgcagaagc attagtaaga
    19681 tggcaagacc caaataaagg tcttatacca ccagacgagt ttattccttt ctttgaaaaa
    19741 aatggtttta ttacaaaact cgaccactat gtttttgaaa aagtatgcag tatgataaga
    19801 aaatggatag ataatggaga aactccaata cctgtttcag ttaatttttc acgtgctaat
    19861 ttaaataata tagatttttt agaaaaatac agaatgcttc gtaataaata caacattcct
    19921 gctaagctct tagaaattga attaactgaa acacttgtat ttgaaaatct ccaaaaatta
    19981 attaatataa tagaccaaat ccatgaagaa ggttttcaat gttcactaga tgactttgga
    20041 agtggatact cttcactcaa tatgttaaaa gaaataaaag ttgacacatt aaaacttgac
    20101 cgtgcttttt ttagctcacc taatgcagat aatattgctg aaaatcatgt aatagaatct
    20161 atagttaatt tggcaaaaaa attaaatatg aatagtatat cagaaggtgt tgaaacaatc
    20221 ttgcaaatgg attatcttaa aaaaattaat tgtgatatga tacaaggata tgttttttca
    20281 aaacctctac ctcaagaaat atttgagaaa atgacttttg gaaaaaaact agaagaaacc
    20341 agtatggata gttccgttta aataaaataa aaaacttgtc tctataaaat aattttaatc
    20401 attttataaa gacaagtctt tttattttcc aatttaattt tattttctaa atttcttaca
    20461 ctcggcttgt tttaaattcc ctttttaaga ataataatac aattatcata gcaataaaat
    20521 ctgcaactgg ctgtgccaac caaacaccat ctaaattaaa ttgaattgga agtacaatga
    20581 ttaatggtat aaataaaata aattgtctta aaagagttaa aaacattgaa tgtttaactt
    20641 tacttataca ctgaaaatac acaggtccta caatagaaat ccccattata ggcaatgtaa
    20701 ttagatttag gcttatacct cttactgcta tatccatcaa atctgaatct ttattaaata
    20761 tacctacaaa tacttctggg aaaacttgta ctactaaaaa tccaaatgac aatatgacaa
    20821 ctgacactat gatagataat attaaagttt tctttgcacg ctcatattgt tttgcaccat
    20881 aattgtatga tattatagtt tgcatacctt gattcaatcc aaacacaggc atcataaaca
    20941 tcaataaaat agatgtaagt gctgtcatag ctccaatagc taaatctcca ccataaacct
    21001 ttagagaatt atttgtaaac atgtgagtta tacttatagc tacttccata gcaaaaggtg
    21061 tcattcctat tgcaaatacc ttctttacta tatctttatt caattttaag ttacattttt
    21121 ttagttttaa attggacttc cctcttataa aataatgtat tacccaaaag aatactacta
    21181 tttgacagag tactgttgct atagctgctc cttttatacc taaattaaat acaaatataa
    21241 atatagggtc caatatcagg tttaatacac atcctacaat cattgtcctt gctgctaatt
    21301 ttggattacc ttctgcccta attgcattat taagagaaaa tgcaactaaa ttaaatatac
    21361 ttccaaccaa tatgacactc atatagtctt ttgcataaaa tattgaatct ttacttgcac
    21421 ccaaaataaa aagtatatct tctaagaaaa ctaatcctaa tatagttata attaaagaga
    21481 taattataga tagtgtcaaa gcattaccta atatcttctc tgcttcttct ctatttcttt
    21541 cccctaactt tatagacaat cttgtgctag cacctactga taccaacatt ccaaaagcta
    21601 ttattagagt aaatactggc atagttacac caagacctgc aatagctata gagccaatac
    21661 cttccataga accaataaaa gctctatcta ctacattata aagtgaagtt accatcattg
    21721 ctattattgc aggtatcgaa tacctaaata ataaaatcca tattttttca tgtcttaatg
    21781 cttcttgatt ctccatgttg tcaccattta caccatgtca taagttatac aaattggttt
    21841 atttgttctt gggtctgttg ctatttctgc atcaatatta aataattctt ttagattatc
    21901 cttcgtcata acatcatatg catcaccttc acaaactatt ttacctttct taatacctat
    21961 catgtggtca gcaaatcttg ctgcattatt aagttcatgt attaccatga caatagttct
    22021 tccttgtttt ttatttagtt catctaataa ttttagtatc tccaattgat gtgccaaatc
    22081 taaatatgtt gtaggctcat ccagtaataa tatatcagtt tcttgtgcca aagccatagc
    22141 tatccatgcc ctttgtcttt gtcctcctga taaatcttcc atatttctat ccttaaattc
    22201 tgcaattcca gtaacttcta aactccaatt tacaatctct ctgtcttctt tactagaatt
    22261 accaaatcca cttttatgag gaaatcttcc ataagaaatt aattctgaaa cggtaagacc
    22321 actaggagct tgagggcttt gtggaagtac agccatatct tttgctattt ctttagactt
    22381 ctttttgtag atgtcctctc catttatata tattttccca ctattagggc taataattct
    22441 acctatagtt ttaagtattg tcgattttcc acaaccatta gcccctatga tggtagttat
    22501 ttttcctttt ggtatattta aatttaaatt ttttataata tctgtgtttc catatgatac
    22561 atttagattc ttagtattta tacagttcat aacaacaccc tttcctaatc atttaccaac
    22621 attaaataaa taaagtacgg cactcctatt atagaaacta ctatacctac tggtatttct
    22681 ataggtgcta gaagatttct tgatatagta tctgctatta aaagtaataa tgttcctatc
    22741 aatgctgata ctggtattaa tcttttatgc tttggtccta ttatttttct acctatatgt
    22801 ggagctatta aacctaaaaa tgaaatacta cctgctactg ctgtcgatac accagataaa
    22861 attactgcat atattataag tcttcttctt tgtttttcta catctacacc aagacttata
    22921 gatacttcat cacccaaatt taaaacatct atatatttag atttataaat agttataccc
    22981 ataaaaatca ctataaatgg aagcacagct attacatatt tccaactggt tccccaaatg
    23041 cttcctgatg tccatgccat aaccctatta aattcctgtg ttgtaaattt caattggaat
    23101 attgtcaaaa ttgaagtaaa agctatgttt acacctatac caataagtag taatcttgat
    23161 gaatttatac cacctttcca tgcaagagaa tatataagaa atgctccaaa taaagctcct
    23221 ataagtgcaa caataggcat tgtaaataca gtcaagttac tcataccttc atatacatta
    23281 ccattcataa ggtatatata aactactaca aacaatgcag cacctgaatt tattcccaaa
    23341 attccagaat cagctaaatc attctttgta actccttgaa gtattacacc tgctgttgat
    23401 aatgcagaac caaccaaaag tgctattact attcttggta atcttaattt aaatattgca
    23461 atttcatgat tacgctgacc ctgccctaaa aaagtcttta taacatccat tggttctata
    23521 gaaaatgagc ccatatttaa actcaataaa aaagtgataa aaataagcac cattaaaatt
    23581 gatataacaa gtaatgtttt agaatttatt tttataatat ttttcatagt acctatttct
    23641 ccccctttct aacaaggtat ataaatattg gcactcctat aagtgctgtt atagaaccta
    23701 ctggtgtctc atatggagga tttatcatcc tacttaatat atctgtatat acaagcaata
    23761 cagctcctaa tactaaagag ctcggtatta tatacttata atctgaacct actattcctt
    23821 tagctatttg aggaactata agacctataa aaataatatt accagacact gacacagatg
    23881 ctccagtcat taatattaca agtactatac atataaatct aacaaagtta gttttttgtc
    23941 ctaatcctat tgccacttct tctccaagac ttaatatagt tattcttgga gccataataa
    24001 tagctaaaat aaatccaata ccaccagcta caaatagcaa ttttatacct tcccacttca
    24061 ctcctacaag ccctcctgat atccagaaac ttaattgttg agataggtta aaatacatag
    24121 atattgctga tgccaaagaa attagcaatg taccaagagc gactcctgca agagctaatt
    24181 ttacattatt aactcttcct cttgatttag aacttataaa atatataaat aatccactta
    24241 tacttgctcc aataaatgca aacatcatta cactaaaact tccatgtata agttgaggaa
    24301 gttttttttg aagtaccagt gatatcgaaa ttgcgaatgt agctccttgt gtaattccta
    24361 taacagatgg ttcagctata ggatttctcg ttattccttg cattattgcc ccagaaactg
    24421 ctaaaaatcc tccaacaagt gcagctgcta ttgcccttgg aagtctaaca tcctttacta
    24481 ttctcatatt tattccatct tccattttta tcaaactatc tataacagtt gatagattca
    24541 tatcttttgc cccaatagtt atggataata caattccaaa tacaagtaca actatactta
    24601 ctaatatcaa caaataagtt atattttttt tactcagctt agtcaattat cctacctcct
    24661 tttccctttt aatcgtcagt ataatatcat ctattaagta ttttaatttt aagatttatt
    24721 aaaaataggt gtgaaaaatg aatatcatta tctcacacct actaagttaa gaattacttt
    24781 gttaattcat ttttaactga ttctaataat aactcttttc caattggatt gtatgattga
    24841 ctgaaaaatg gagcagcatc aagtattgtt acattaccat cttttactgc tcttatttga
    24901 gcccagatag cactattttc taaatctttt ttatctgctt ctgtagcaat tacaactata
    24961 tggtctgcat ctatatctgt taaaccttcc atacttacca ttggtagtgt tattccatcc
    25021 tgttttggca tattcttagg tttagctaat ttcatatcat cattgattag agtacctatt
    25081 ccaccatcac taaataccat aaattgacct gaacttgcca acactggtaa ataagttttt
    25141 tctccattct tttctataac ttccttacct aactttgtag ctttttcatc atacttctgt
    25201 agccaacttt tagcttcttc ttctttgtca aataactttg aaacatcagt taacttgctt
    25261 ctccagtcat ttgcataatc tttcatcata actacaggag ctatttcttt taattggtca
    25321 tatatttttt cttgtctttg actcattatt attaaatcag gatttacttc taatatagct
    25381 tccatatcca tagtatccat cattgagtgc ccaactatct ttacaccttt aagctcttct
    25441 cttatgtaac ttggtaattt atcagtttca tatgagtcta catttgctgt tgcaacaggc
    25501 ttataacctg ctaataatag ttcttcacta cttcctgata tatcaactat ttttttagga
    25561 tttgatggta tcttaacctc tcctttaaca gattgtacaa ctcttgtctc tgatttatct
    25621 ttatcctctg tattcttatc tgaacatgct gtaacaagta ctaacgtagt tataatagat
    25681 ataaatattg caagtgattt tatttttttc atgattactt ctcctacttg tttattatac
    25741 ttacatgata ttgattatca taagcacata tttattatag catttttcta tttttagtca
    25801 atattatttt gtaaaaggat aaaaattcct aattaatctt aattatatat agcttagata
    25861 aggttttctt atattaaaca gtattttttt aaagaattgg acacaaatac aatgactttt
    25921 cagttaaaca atgataaaat gcaatttata gataatgaat ttagtaagga ggattcaaaa
    25981 catggtacat ttagtatatt gtgataatgc aggaaaaaaa ggagaaaagg tcttagataa
    26041 aatcttggct ggaacaaaaa caatggttat tagaggtgct gctggtagaa aaataccaca
    26101 tagtagagta tttgaagatg agttacttta ttttatggaa aaaggaactg caaaaatatc
    26161 tgcaaaagcc actgttaaaa aggtagaaaa ctatgttaaa ttaacagaag atgaaatttc
    26221 taaaatttta aatgaaaatc aaccaaaatt aaatttatct gaaaagcaaa aagttcgttg
    26281 gcataaaaaa tgtttatgct tagttgaatt tgaaaatgtt gaagaaataa cacctcttga
    26341 ttttgaccat caaggaaata tggatgattg gcttattgtt gaaaaaattg aagatgtagt
    26401 tgttggtact agtatacctt acaattatga gaagtctaaa ttttaaagta tcaataacat
    26461 aatactatat tgctaaactc tacttaaata aaaaaagagt gcatttaata attataaaaa
    26521 ttatttttat acactctttt ttaataataa ttttaaatta cttactttct aatctttcat
    26581 atacatctat aaattcttct gctaattgtt gaaaattcat agctgtcatt aaatggtctt
    26641 gtgcatgaat aagtataaca cttatttcag ttttattacc attagcttca ttttgtatca
    26701 attctgtttg gaatttatga gctttatgaa ttgattcttt cgacttttca atacattctc
    26761 tagctttatc tatattcccg ctctttgctt cttttattgc ttcaaatgct agccctttcg
    26821 catcacctgc aaatccaatt attccaaaag atatctctat catcttctca tccatttatt
    26881 cgacctccta aatatctcta tagcttgata tgactaatcc attcttttct aaaaattctt
    26941 tgactttttt agatgtaagt atcttgtgct ctttcgttct atcaatagca taagatgtag
    27001 aatttaaaat ataatcatct aaaaaagctg ggtgagacat aatatccact acatcatact
    27061 tcattatttc gtctatattt tttataaaat attcttcact tacattttcc ttataaaaac
    27121 tatctattaa aggaactact ttagaatact ctaaattata ttcaaatcca cctcttattg
    27181 gaagattata cttggttaca atcttctcta ttactggctt taaacttaat aaagtatgga
    27241 tatgatggtg actatctaaa tgagatatat ctattccaag cccttttacc ctatctattt
    27301 gagcacaaag ttctctataa atctcatcac aatccatttt ttcaataatt tcgtctgtaa
    27361 tcctcctaat aaaaaaaccg tctttatcaa caatagtttt taagttagat aataatggtc
    27421 taccacaact taaagtcata tgaactccac aatttaaagt tttattttct ttaagtaagc
    27481 ctactccatg ttctacccca ggcatatttg ccatcataga agtagaccta actataccat
    27541 tattatgagc tgaaattatt ccatagttca cagcttcaca atatccaaaa tcatctgcat
    27601 ttattataat tttagtcata ccataaccta ctttctatag acaactatct attaaatcta
    27661 ggaagataat ctttatgagc ttccaacatt tcgtctaata tagtttttgc taagtcatca
    27721 gatggaatca atgggtttat acaaagtgcc aaaagagcag attcataatc gccatataca
    27781 gctgctttag ctgccaatac ttcaaatgat ttgatttgac ttactaatcc atgcacagaa
    27841 tctggtaaat atcctatact tattggtttt ggaccatttt ttgttattac actacttact
    27901 tctaccgctt gttcatcttt aaaatctctt atagaaccct tatttaatgt attaactact
    27961 tggatatctt ttttgtcgtt gtatatagaa cttattaaat tacaagcagc atcactatag
    28021 taagcccctc ctcttttttc aagttgaggt ggttttacat ctaatttttc atccttatat
    28081 aattcaaata attgttcttc caattcttta actacttgac ctctagcttt tccctcttta
    28141 aactctctca actcatcctc aagcatttct ttagttttat agtagtatct atgatatgga
    28201 caaggtattg cacccaaaga ttttacaaat tcagcattaa aatcaattgc ctttatattt
    28261 tgcatactta tatctgcttt gacaaattta tcaattgctt cttttgtaac atcttctcca
    28321 tctaaagata catttagacc ataaaccata tgattaagac ctgcaaaatc catacttatt
    28381 ctgtcacttt ctacattaaa tagtttggca acatcatttt ttaaatgaat tggcacgttg
    28441 catagaccta tatatcttct aaaattagta tatttgaata cagcctctgt tattattcct
    28501 gttggatttg taaaattcac catccatgca tttggacata attcttccac atcttttatt
    28561 atatcaaata taactggtat tgtgcgaagg gctttaaata aacctccagc accattagtt
    28621 tcctgcccca taactccatg agataaagga atagtttcat cttttattct cgcatctaaa
    28681 agacctactc taagttgtgt tgtcacaaag tctgcatctc ttagagcttc tcttctatct
    28741 aaagtaagat ttattttcat atctactcca gcttttttta ccatcctctt agctagattt
    28801 ccaactatct ctaatttttc ttttccagct tctatatcta caagccataa ttcctttaca
    28861 ggtaactctt catatctatt tataaaaccc tctacaagct caggagtata acttgaacct
    28921 cctccaattg taactatttt tagtcccttt ttcatctcat tataccatcc ttacctataa
    28981 tttaatatac tctacaaaac cccatacatt tatttaaaac tctatacatt tatttaccga
    29041 aataaaacta cctagttgtt aattttcaat ttattagctt ccattttttc agacgctatg
    29101 acaaatggaa tatatattaa tattgataat attaaattaa atagtgctaa agctccacca
    29161 gtccagtcac ctgtagccat tgctccacct aatataggag gtgttaccca aggtatttta
    29221 ggtaaaatta ctggtgctac taatccaaaa tctattgcta agtatgatac agcactacat
    29281 attactggtg caagtacaaa tggaatcata aatataggat ttaaaactat tggaagtcca
    29341 aatatcaatg gttctcctat gttaaatatt gcaggtggac cacctaatac tatcatctct
    29401 ttacgtcttc ttccagctat taataaagct actattaatc ccaataccat tccagaacca
    29461 cctaaatata caaatgcatc aaagaatgca cctgcaagta catggtatcc ttccgttgct
    29521 cctgcttgag ccaaggcagc attttctcca cctaatttca taagtatagt ttctgtaaaa
    29581 ggaagtgcta tatttgcacc atgaagtcca acagtccaaa gtatatgaat tataaatgca
    29641 ataagcattg tagaacctag actatcagct acatttgata gtggtgctcc taaataagta
    29701 tttaaaatat ctgttaagaa acttccatta gaaagcattt ttataaatat tcctattaca
    29761 gtaaatatca ttatagttaa cattcctggt attaactttg caaatgatct tgaaactgct
    29821 ggtggaactc catctggcat ttttatagtt aatttcttta ccttagataa tcttacaaat
    29881 atctcagttg ataataatcc tattaatata gaagagaata aagctgctgt acctaagtag
    29941 gtttgttgta tcattcccca tccttcaact actgtagttc ctccctcagg aactatttta
    30001 ccaatttgtg gagccattat aaaaaagata gataaagcta taagcccaga ttgtaaccca
    30061 tcactctcat aagattttgc tagaaaataa gcaacaccta ttactaagaa tactgccatt
    30121 gtacctattg ctccccacca aaggtcgcct ccaaatgtgg tccagttttc tccaaatata
    30181 cttgccatca aatttttgta tgcttccaat ggtaagttat ttatcaatgt tcccaatgca
    30241 cctaccatag ttataggtat cattactata aatgcatctc ttactgctac taagtgcctt
    30301 tgagctccta ttttagcagc tacaggcact atatatttat ccataaatga catgaatttt
    30361 tccattttac atcctccagt acaactttat tttgaactat ttaactctac agctctatct
    30421 aacacagctt ttccattaca tcttccataa tccatcatat ttataacttc tactggaata
    30481 ttatatggtt ttgccatttc agttgctttt cctaatacat atctaacttg aggccctaaa
    30541 agtaagacat cacaattttc tatttcattt tttaagttca cttctgatat agcccatatt
    30601 tttgcttcta taccgttttc ctttgctgca tcctccattt ttgtaactaa taaactagtt
    30661 gacattcctg ctgaacaagc taacattatt tttatcattt tttcccctcc aaattagatt
    30721 cattaaatat tttttaagtc ataagttttt taaactatac atttaaaata tgtttttcta
    30781 ttaatttttt cttaatgtat atatcttatt tttaaatgta tatacattta attcttgata
    30841 ttattatata ccaaatgtat atacatttca agaaaacttt ttcattttat ttaaatttta
    30901 ttcgacaaca gcattcaaag ttaatgtata tccatagtga tatgtgtgtg agtactcaaa
    30961 aatctcacta ttttgtaact tacaaatttg gcttatatat aaaagtggct ctggttcttc
    31021 taattttaga ttatccatac actcttcatt cgatattaca gcttgtactt ctctctctga
    31081 catggctatc ttgtatccac acacttcttc aacaaaatta taaagagaat tttcacaatg
    31141 agtcttattt aaatttggaa ataacttaac tggcatataa gtaatcatat atgaaactct
    31201 tacatcatta gctaatctta ctctttttat ttcccaaaca ttttcttcta aacttatatt
    31261 caacttactt gataaatttt catctgcctg aactatttca agagatacca atatattaga
    31321 tactttatag cctttacttt ccatctcttt agtaaatcca cttacagacg atatattatc
    31381 atatatatta tttgcaataa ctatactacc tacacctctt tgtctagtta tatagccttc
    31441 tttaactaga ttattaagtg cagaccttac agtcattcta gttacattaa attcttcact
    31501 cagctgtttt tcagaaggga ttaaatcccc ttctttcaaa ctgtctgaat ttattaactc
    31561 tctaacatga ttttctataa ctttataaat tggctctttc atttaattca ctccttatta
    31621 aatcttttat ataataagat aattttatat acaaacaaag aaaatgtcta tacatttaag
    31681 ataatttaaa ttttattcag aaatcttgta caactttccg agttttaata aagatataaa
    31741 tagacttatc attaatatac cttttaatat acttgataag gttatactcc accatacacc
    31801 atttagaccc aacatttctg gtctagatat aaagtaagcc aatggtactc ttgcaccagt
    31861 cagtatagta attataatag aaggtatata ggttctccct aatcctttga aagctcctgt
    31921 tgttattatt tctaaacaca taaaaaattg agaatagcct aatattttaa gataaactgc
    31981 tcctttttct attgcctcac tttcatctat aaaaatagaa aatatactct ctcctgcaaa
    32041 cactaatact aaagtagtta ttataccaag tacagaagat acagttatag ctattttgca
    32101 acctttattt attctagaat acttcttggc accataattt tgtccaacaa aactgcctaa
    32161 tgcaactgat agcccttcag ccgtcatcca ggaaattgct tctatttgtg agcctacttt
    32221 ttgtactgca acagccacag gcccccatga tgctactatt actcctaata gcattgagaa
    32281 tactgtaaac ataccacttt gtattgctat aggtaatccc aatttataca gaactttata
    32341 atatttagtc tctatttctt taaatagtct taatttaaaa tattcttctt tacttttaac
    32401 tattatatat ataaaacatg tagtaactac tatctgagca aatacagttg caatagcagc
    32461 tcctgcaaca ccaagttttg gagcaaatcc ccatccaaaa attaatatag ggtcaagtat
    32521 aatattagtt attaatccta ctgtattaat acgaaaagga gttttactat ttccaagacc
    32581 attgaaaatt gcagtaaata ctggattaat aaaataaaat accataccta gagctactat
    32641 tatcaaatac tgtctagaca tggttataac ttctaagtct cctaatctaa aaaaacctat
    32701 caattgttta ttaagtacta ttaaagaaat tgtatatatt attgctagta taatatttat
    32761 ttctattgct gacttcatat atgatttagt agtgcttata ttatttgcac caatactttg
    32821 tgctactttt atctctccac ctacttttga aatcataata aatgccattg caagccatgg
    32881 aaaaaaacca gctgttccaa ctgcagctac tgctttactt ccggctttac caacccatat
    32941 catatctatc atgttatacc ccatctgtat aaatgaagtc cccattattg gaagtgatag
    33001 cttaagtaat ttttctgtaa ttcttccttc tgttaaatta agtctgtctt tcattttcca
    33061 cctcttttat attaaattta tttgcttata tagctagatt aatcatttta tttgattgag
    33121 ttcactatcc ttcaatttat attatcatat ctcaggattg catctcaatc atacttatgt
    33181 ttaagataca gtattttacc tatctatgat aaaattaata cttatgaata taaatctagc
    33241 catttcatca atacatattc taacctttaa cttaacttca attaagtata catatcgtaa
    33301 ccaattagaa tattttatac ggtatcttcc attaataaac aaccaatatc aatgatttat
    33361 atataatttt atttatacat aaaatagtat actataataa taaaaaaact atatttgaat
    33421 gctttaagca tgtttactaa ttgtaatttc cacacaaatt tgataatata tgaatatatt
    33481 atggtgaaat ttatagatat atattaatta tggggagatt gtgggaggtt cttttcacaa
    33541 gacatttaag ctatgaaaat aaataaaaaa ttcaatagag ttgatatgca ggtttcaata
    33601 cttactgcaa ttattgtaat aatatcttcc tcatgtgtat atttttttaa ttatacacta
    33661 acttataaga gcatgatata ctctttaagt gagaggtcaa aacatatcta tgaatatgta
    33721 gaaagaaata ttgataaaaa tacctttact gaaattaatt ctaaagaaga ccaatcaaaa
    33781 aagtcttata aaagttctaa aatcctttta gaatctgtaa aaaatactac tggagtcatg
    33841 tatctatata ctgctaaaaa aacaaaagat ggatcactag tatatgttgt tgatggttta
    33901 aattcatcta ggagtgattt tagaaatgct ggtgatttga tagaaaaaga gatttgggga
    33961 gaccttaaca aagcattaga taatcatata ttgttaccta aaaagattaa atcaacatct
    34021 tggggataca tatttatatc gtattttcct attcacaatg atgttggtaa ggttgttgga
    34081 gtaataggaa ttgaatttga agcaaaacat caatacaata catttaaatt tataagtata
    34141 gttacaccaa taatagcatt attaacttgt ctaatatcag cattgattgc agtgattcta
    34201 tttaaaagaa tatctaatcc tacatatact gattttgcta atacagatta tttaacaggc
    34261 ctaaaaaata gaaatgcttt tgaaatagat atgaataact taaacaattc aaatctaaag
    34321 aatctaatat ctatcatatc aatagattta gacggtttaa aaaagattaa tgataaattt
    34381 ggccatcaga caggagactt atatattaag caagcaagta aaattataca atatgtaata
    34441 aataaaaaat atatagttta tcgtattgga ggtgatgaat acataattat tctaaaaaat
    34501 ttatctcaca aagaaataaa taatattata aataatatta atttaaaaat agttgaagaa
    34561 aactcaacta atgaattaaa attatcaatg tctattggat atgctatttt tgatgaaaaa
    34621 gtagatgttt ctttatttga tacatatcgt cgtgcagatt ctcaaatgta tgataacaaa
    34681 aggaaaatta ataaacaata tcaataataa atttaaaaag tctattcttt aaataataaa
    34741 tcacaagaat agactttttt aaatttgtgt ttttataaga taaattattt aattaaagtt
    34801 tgttcttttt tctaaacgat tctttataaa ttcatatatt actttcacta atatatttgt
    34861 gactaatatt aacacagcta gtgatgctgc ctgtgcagta ctccccttat catctagttg
    34921 tacaattgat attgcagcta cttttgaact tgcagtatat ataaaaacta atgctgatat
    34981 tgtcaccatg gagtttagga aaaagtacat aaagttctct aaaactgctg gcaaacaaag
    35041 tggtatagtt acgttcttaa gaattgatgc gctgtttatt cccattgatt tagctaccat
    35101 atccaattct ttatctactt tcttaatagc agtagtagct gttataaatg ctactgaaaa
    35161 gaaatgaact atattacata taaccattat aagtaatgtt ccatataatc cattaaacaa
    35221 attcttaatg tatattgtat ccataaaatt aaactctaat ttattaaaaa ataatacata
    35281 agctatacct aaaactaatc ctggcaatgc cataggcaat gtacttaaaa aatacccaaa
    35341 tgattttaaa tatggagctt tttctgtttt ttcattcaaa tatgcaaata agaaaacaaa
    35401 gctcgtccct agaatagctg tcaatagtga catttttatt gaatttataa atggtttaat
    35461 cccatcaata ctgtttttca tagaaaaatg ttccagtgtc agtcttaaat catatggcca
    35521 aactgatatt aatgaagata agattacagt taacattaca gctattatac caaatgcaat
    35581 aattcctaca acccctccta aaatactatc tcttaactta ttagttttaa ccttatatgg
    35641 aattgacttt gaagttatat atgaattttg atttttttca atttttttag ttactataaa
    35701 cgatattatt gcaggtatta aaagtaatat tgatacagtt gctcccattg gcatattctg
    35761 ttgccctata atttgcttat atacatctat tgataataca ttaaactctc ctcctatcac
    35821 ctttggagct ccaaaatctg taaaacataa tgtaaacgat acgaatgcag aacttattaa
    35881 agagtatttt atacttggta ttgttatgct tataaattct cttatcttgc ttatccccat
    35941 agtattacta gcttcatata gtctataatc ggttgaactt agagatatat aaagtatttg
    36001 aaatgattga ggaaatacat atataatttg tgccaatatt attcccactg ctccatatat
    36061 tggaacatgc actccaagta aattagttac caaaccttga tttccaaata aatatactag
    36121 acctattgca tgcatcattg ttggagcaaa cataggaaac atagctaatg atttaaaaaa
    36181 acctttaaat ttaatctttg ttctagttaa tgcatatgca tatactaatg caagtgttag
    36241 tgatattaca gttgatataa ctgatacaaa tactgtattt tttagagaat ttaatagaga
    36301 ttcagaagcc aagtattctt gaaagttttg taatcctaca aagactccat ctttatctaa
    36361 aaaagcctgt acaaatagat atattatagg tgctaataaa aatattacta aagacataac
    36421 taatactgta agcatacatt tttttatatc taaattcatt tttgatttaa gacttatact
    36481 tttagaactt atagcattat tattcataca cttatgctcc tttaactttt ggatacttta
    36541 aatagtattt ttcgtctata cttattttta caaggtcacc ttctactaaa tttaaattaa
    36601 cccactcttt tatagaagca tcacttatta tgaaattttc atttaattta tcttttattt
    36661 caactctcaa taaattccct ctaaactcta tatttgatat tattccttgg tagttttctt
    36721 ttgtcgattt ctctatttga acatattctg gtcttacagt taatatagaa tctccattat
    36781 caaaacagtt tgtatctcct ataaattgag ctgtaaatag gttttgagga ttttggtata
    36841 tttcttctgg agtccctatt tgcattatat ctcctccatt tagaatagct attttatctg
    36901 ccatagttat agcttcttct tggtcatgtg ttaccattat tgtcgttata ttaagttctt
    36961 tttgtaatct tttaatgtcc attctaagct tgtgtctaac ctttgcatct aatgcggaca
    37021 ttggttcatc taaaagtaaa aactttggtt caagagctaa tgctcttgct attgcaatcc
    37081 tctgttgttg cccacctgat aatgctttag gatatttatg agcttcatta gtcaatccaa
    37141 ctgtttcaag tacttcctta actttattat ctatttttcc ttttgaaact tttctctctt
    37201 ttagtggaaa agctatattg ttataagcag tcatattcgg aaataatgca tatgattgaa
    37261 atactatacc aaaacctctc tttgatggct ctaaatttgt aatatcttta tcctggagaa
    37321 ttatagttcc gctatttaca tcttcaagac ctgctattat tctaagtaat gtagttttac
    37381 cacatccact aggtccgagt aaacatagaa attctccttc ttcaatatcc aaactaatat
    37441 tatttaaaac ccttttttgg tcataacttt taaacacatt atttattttt aagtaactca
    37501 tatcttatct ctccttatat agttattttt ttattttaaa ttttatatcc tactttttct
    37561 tctaaaattc tttcatctaa attattaatt taaactttac ttactttcag attttgaacc
    37621 gtattttgat tcccaagttt taagtactgt acttctgttt ttggcagcag agtttaaatc
    37681 aacttctttt ataagctgtt caaatggttg tttagaataa ccttttggaa ctggattatc
    37741 tgtctttatg gaagtaactg ctacttcatg tgcatatcta tccatagctg aatcacttat
    37801 tgcccaatct aaaaatagct ttgcttcttt ttttatatta tcttttttaa ctaaggcatt
    37861 tgcttctaag tcccagcctg aaccttcctt tggaaataca acctctatag gttctccact
    37921 agataattgt tgtgtagctc tatatccaag tgtaatacct attggatact ctccagagcc
    37981 agctaacttc gcaggttttg aacccgagtg tgtatatgtg gctatatttt tatgtaattt
    38041 atccatgtaa ttgtaagcat ctttttcacc catcatttga ataagccctg atactgtaag
    38101 ataccccgtt ccagatgatg ctggatttgg cattgttata agtcctttat attctggttt
    38161 tattaaatct tcataacttt ttggaatatc tatacctagt ttcttaagtt ccttataatt
    38221 aactactatt gctgtctctg ggacactcat tcctacccaa gatacatcat cttcagtatc
    38281 tttaaatttt ttatcaactt tatcataatt tttaggctta tatgcttcaa gtgcaccatc
    38341 tttttttagc tctatcatgt tcattgctga taatccccat ataatatctg cttgtggatt
    38401 atctttttct gctaatagtt ttgaagctat tacaccatga gaatccataa ctatctctac
    38461 atctatattg ggatactctt ctctaaaacc ttccatataa taagctatct gctcttcctc
    38521 tagtgctgca tatattgtaa gtttattatt tccttttcct tgagtagttt gtggtgttgt
    38581 tgatatgcaa cttgtcataa atgtaatcac tacaatgcac agacaagtaa atataaattc
    38641 tttcatatct tccccctttg tgttttttcc tttttttatt tgttttttat tgtttttgct
    38701 tatgtttata taatacacct aatttgttaa tataaagtta agtgtaaatt aacagttcgt
    38761 taatttttag aaatttttgt taaatatatt atttttagtt aaatacagtc tgtttttaaa
    38821 caatatatta atttttatag tatctacatt ttaatttaaa cataaaaaat aagtctaacc
    38881 tctataaagt agaatgttag acttattttt ataagtattt aattatacaa ccaaatcatt
    38941 ttttttcttt tcttcaagtg cttttttaag caccatatcc ttaaccttca taagtctagt
    39001 agtacttgct ctctcatttt cttcaagctt catagctata tacttaatag tctcaatgta
    39061 atttggtatc attacatttt caagagcatt aacacgtcta cgtgtctttt ctatctcttg
    39121 tgcaagaagc tgtgcacttt tttcactttc tgcaagtctt aaaagtggtt gcatagcatc
    39181 tgagaaagct tccatagcag aatctaattc acctgatgta aaagcaagac cataaggata
    39241 aatatcactt tgattgtttt cagttttaaa gtcaaacaca ggaacatcaa cactcattat
    39301 atttctcgta gaaacatcta ctgaaactga ttgctttggc atcataagtg ctgaacccag
    39361 atactcctga ctcataactg ctcttgcgat tataaagttt ttataagcag tatcaagagc
    39421 attttctgct tcttcacgca atcgcttatt ttctcttaca atctcaagaa attgtttcat
    39481 aagttcatca agtttatctt taagaagctt atgacctctt gtagcagttt taaggagttt
    39541 cttaagtcta gtcatttcca tacgtgttgg atttatattt aatctagcca cagatatcac
    39601 tccttaggca tatatttttc aaaatattca tcacggatac gtttaagttc actttttggt
    39661 aacatactaa gaagcttcca tccgatttct aatgtctgct caatagttct atcagttcta
    39721 aatccttgtg aaacatattc tttctcaaat tcatctgcaa acttagcata cattaaatca
    39781 atctcagaaa gtgcagattc accaagtata gccataagtt cttttgcatc cttacctctt
    39841 gaataagctg caaaaagctg gttcattgta tctgcatggt cttcacgagt cttaccttta
    39901 ccaatacctt tatcttttag acgagaaagt gatggtaaaa catctatagg aggctgtata
    39961 tcttttttat aaagttcacg gctaagtata atttgccctt ctgtaatgta tcctgtaagg
    40021 tcaggaattg gatgtgtctt atcatcctca ggcattgtaa gtattggaat catagttata
    40081 gacccttcat gccctttcat ttttcctgct ctttcataca tagtagcaag gtcagtataa
    40141 agatatcctg gatatccacg acgtcctgga acttcttttt tagctgctga aacttcacgc
    40201 aacgcctctg catagtttgt aatatccgtt attataacaa gtacatgcat atcttgctcg
    40261 aaagcaaggt attctgctgc tgtaagtgcc attctaggtg tagatacacg ctctacagct
    40321 gggtcatttg caaggtttac aaacactaca gaacggtcga ttgcaccagt tgctttaaaa
    40381 tcgcttataa aaaattcagc atcttcaaaa gttataccaa ctgcagcaaa tacaacggcg
    40441 aacttagaat cagttccacg tacttttgcc tgtcttgcaa tctgaactgc aagattggca
    40501 tgtggcaaac cagaccctga gaatatagga agtttttgcc ctctaaccaa tgtattaagt
    40561 ccatctatag cagagactcc agtttgtata aactcggccg gataatcacg tgctgctgga
    40621 tttattggag ctccatttat atcaagtctt ttatctggta taatttctgg acctccatca
    40681 ataggtctac ccattccact aaaaacacga cctaatatat ctggtgaaac tccaaagtca
    40741 agactctttc ctaaaaaacg aactttactt tcagcaaggt ttatacctgt tgagctttcg
    40801 aaaagctgaa caagtgcagt atctccattt acctctagaa ctttgcaacg gcgaatttct
    40861 ccatttgaaa gttcaatttc tccaagttca tcaaatttta caccttctac gccgtttatt
    40921 aacataagag gaccggctac ctcttgtatt gacttatatt cctttatcat agttcttcag
    40981 cccccctctt tttaataagg tctgcaagct cgttatctat ttcatcttca attgaatcgt
    41041 aagctgcatc tatatcgcct tcttctacat acttagcacg accaatcttt tcaagaactg
    41101 aaagcttgat aatatcgttt atagtaacat tttgcttgat agcatcttgt gctgacttat
    41161 agaattttaa tataagtccc atcattctat attgtttatg aagtgatgta taagtatcaa
    41221 cttcatggaa agagttttga tgaaggaagt cttctcttat agaacgagta acctctagta
    41281 tcaatctatc tccatctgaa agtgaatcaa ccccaaccaa ttgaactatt tcttgaagtt
    41341 cagattcaac ttgtaaaagt ttcatagctt gagctctttg agctgagaaa ttatcattta
    41401 catttttatc tgcccataca tcaacttttt cctgatagag tgaataacta gtcaaccagt
    41461 taattgctgg gaaatgacgt ttatatgcta aagatgcatc aagaccccag aatactttta
    41521 ctatacgaag agttgcttgg ctgacaggtt ctgatgtatc accaccagga ggagaaactg
    41581 caccaattgc tgtaagtgtt ccttctcttt catccatacc tagacaattt acctttcctg
    41641 ctctctcata gaattgagca agacgtgaac ctaagtatgc aggataacct tcttcaccag
    41701 gcatctcctc taaacgacca ctcatctctc taagagcctc agcccatctt gatgtagagt
    41761 ctgccataag tgcaacacta tatcccatat ctctaaaata ttcagctatt gtaataccag
    41821 tgtatataga agcttcacgt gcagcaaccg gcatatctga tgtatttgct ataagcacag
    41881 ttctttgcat aagtgactcg cctgttcttg ggtcttttaa ttcaggaaat tcaagaagaa
    41941 catctgtcat ttcatttcca cgctcgccac agcctatata tacaactata tctgcatctg
    42001 cccatttagc aagctgatgc tgtgtaacag tttttccact tccaaaaggt cccggtatag
    42061 ctgctacccc accttttgtg attgggaaaa atgtatctat aactctttga cctgtaacaa
    42121 gaagtgaatc tggtgttttc ttttctctat atggtctttc ttttctaact ggccattttt
    42181 gcatcatagt tacaggtata tcattacctt tttcatctgt aataacacaa actgtatctt
    42241 caacagtaaa ttcaccagaa tatatttctt ttatagtacc cttaactcca taaggaacca
    42301 taattttaca ttctactact gctgtttctt gaacgccacc aattatatct cctgctacaa
    42361 ccttatcacc aacagatttt ttaggtttaa attcccactt agttgttcta tcaagtgaag
    42421 atacttcaac acctttagtt atattagtac ctgaaatatc atacattttt tcaagtggac
    42481 gttgaatacc atcataaata gttgaaataa gacctggtcc aagttcaaca ctcataggca
    42541 ttccaagaga aactacttct tcccctggtc ctagaccgga agtttcctca taaacctgta
    42601 tagaagcttt atctccatgc atttctataa tctcgcctat aagatgttta tctgaaactc
    42661 ttacgacgtc aaacatattc gcatctctca tgccctccgc tacaacaagc ggtcctgaca
    42721 cctttacaat tgtgcctgtt ttcatattct ctcacctgca atcatatact ttatcaaaat
    42781 ttaaatattc cctcctatat tttattagca catatattta gcaccaataa aatttgagat
    42841 tgtcttttat tcaactttca tctattcttt aaataatata tctgcaccta tagcacgttt
    42901 actagattct cttacttcat tcatacctaa tcccatactt cctccaatac ctggaataac
    42961 tataatggca ggaagttggt aatctttgta ttttgctatt gattcttttg caagcaatga
    43021 tgcctgttct gtaatataaa ttatcgcata ttcatcttca accaacttat gtatactttt
    43081 tttaatttcg tcactatcat aggctgggaa gatatctatt ccaagagcta aaaatcccat
    43141 gatgctatct ttatctccaa ctactcctac cttatacata agcatccctt agcctttctt
    43201 ttatagtatc tgcgtctata ttattaagct tactagtcaa aataattcta attgttttta
    43261 tctctgattc tttagcatat aaatatgcaa ttagtggttc taatgtaaga ggcttaaatt
    43321 ttgctcctct aacatattcc ataagatagt catcacaaag tttttcaaag acagtaaaac
    43381 cactatccat gccattttta cagacatcag agtaagaagt agatttaaaa catgatgctg
    43441 gagtatctga ggaaaaagct tctaagaatt tttccttatc aagtgagcca cctgaaacaa
    43501 acacattcat aaacatatca aaatctttct tcatattttt gactcttata aaactcttta
    43561 gatttgttaa atcacaaact ttcataacat aatctattac aaatttgttt tggctttctt
    43621 tagcagtttc tttcatactg gtaaaggttg ctttatctag aacaatatca accatctggc
    43681 cattttgtgt cttattataa acatcaaatg cctctaatat agctgatgac attatcttag
    43741 gtaaatcact aaaactacga tttgctaaag attctctaag cttaataaga ggaattgtac
    43801 ctccatcaat taaatatttc tcaccatcta caccagaaat ttcttcttta atcagcactt
    43861 ttaaattgtg atagtcattt ttataaagaa atacatcaac aaatttttct tcaggagcta
    43921 gacttttaag aatttcatat gtctttgata attcttgagt caaaatattc tcaaaattat
    43981 gtatattatt atttgatgta tcagcataac ctgcctcagc tataattctt aaagagtctt
    44041 cagggctttt ggcttctgcc atttgaataa acatttgtct gttaagaagc tgtttttcaa
    44101 gcactcttat tcgtgcaacg gcatagggat aagttttttc ttctgccatt ttttaacccc
    44161 tccttaatta aacaaaattt ctgctgcaat ttgttcaata tattcatgct ctactgcaat
    44221 tatggcttca aatgaataat tgtactcaat gtcacctttt ttaactatga aacctccagt
    44281 aatatctcta gtttcatcag acactttaat gccttttttt gatagcacat cttttaatgt
    44341 ttttttatct tttttagata atactatctc tgaatcatct gtacaatttg atttttcaat
    44401 catgtttagt ataatctctt catattcatt atctttaaga tttaaaagtt tgtttttagc
    44461 ttctaaaatt acctcttcta aaatctcttg tttttttgat aatatttgct ttttagcctc
    44521 catataagct cctgatattt ccttagaagc cgctttttct gcttctgcct ctgcaagctt
    44581 tgtataggaa gccatttcct tttcagcttt ttcattagct gaatttactt ttaaatctgc
    44641 ttcactttta gctttatcca atatttcttg tgcttcttgt ttggcatcag ctataattct
    44701 gtcaatcatt ttttgttcat tacccatagg agtgcttcct ccttttcatt ttttttactt
    44761 atgaattata atttataatt aaccaattgc aattccattg taagctaaga aagaaacaag
    44821 tagtcctaaa agagcatatg tttcaactat tgctgcaaat gttatagctt taccaagttc
    44881 ttcaggtctt tttgcaacta ttccaactcc tgctgctgct gctttacctt gtgctatacc
    44941 tgatactaaa ccaactaaac caattggaag tcctgccata agaagttgta aaccttgagc
    45001 tgatgttggt attgcatctc caccaataat tcctgctttt gataaaataa ggaaagctat
    45061 gataaatcca tacagacctt gtgtaccagg aagaagttgt aaaactagaa gttgaccgaa
    45121 tttactaggg tcttctgtta caacacctgc tgcggcttga cctgctattg aagtaccttt
    45181 agcagaaccc atacctgcaa ataatgctgc tatagcagct cctgatattg ccaaaaattg
    45241 accatttcct aacgcgttta aaaattccat atttacatct cctttttatt aatgcttgta
    45301 tattttgttt tatacttaaa cggaacaaaa ggaactcctc cgccttcata aaacttattg
    45361 aaaaattcta catactgtaa cctacttgta tgaacatatg cacctagtgc atttataaga
    45421 aggtttattg tgtgtcctac aacaccaatt acaactgcaa gtatcggacc agctatagct
    45481 cctaaaaggt tgataacttg tgctataact cctgttgtta aacaaagcgc cataattctt
    45541 gtataagata atatatcagc aaaataactt gttataccat agagacttga aaatccttta
    45601 aaaagcttaa caggaatacc tttaaagctt cttccttgag taagtacaag acctatagca
    45661 cctataattg ctaaatattt tcctacttct gaaaatacac ctatatcccc tgcaacaaaa
    45721 ggtattataa gtagacatac acctgttata cacaaatacc aaaaacctat gtcaaagatt
    45781 gcatctatta cctttccact tcttattaat gtatatgctt ttattcccaa acctacatat
    45841 atatggataa taccgaacag taacgacatt cccataagtg tcataacatc ctctaaagga
    45901 ttaattaatg cttttatagg aattaaatcc cccaaaatac ccccgaatat aagaccccat
    45961 acagtagttg aaactccaca gataccaata agtttaatta aatttccttc acctttttgt
    46021 atctttaatt tatatacaac aaatccacag actactgcaa ttattaaccc atatgcggcg
    46081 tcacttagca tcattccaaa gaatacaaca aagaagaaag ttaaaattgt atttgggtca
    46141 atatcttttg tacttggata gctgtacatg ttagttacac tttcaaaagg tgttactaat
    46201 ttattgtttt caagcagaat tgggaagcct tcttctttac ttccttcttc agtctgaaca
    46261 caacaattaa acttttctgt aatttcttta attaattcat ctgccctttt gcttggtaac
    46321 caaccattaa gacagaatgt tgtttttgtt ttgacaagtc tctctataat ctttttttgg
    46381 tctctttcta tagtgtaaaa atcatatagg ttttcaagca tgtgtatact atctgcatgt
    46441 cttttaattt cctctgtttt gtccttagaa acgttttcct gtctaattat catttcattg
    46501 tatttcttga tagcttgtaa cggtgtacct tcttcatttg gtaatgaaac cactgaaaaa
    46561 ttaaattctt ttaaaacttc taaaacatca tcaaaagttt cttttaaagt aattaaataa
    46621 acatagttca ttaacttgtc agaattgaca atcccaataa cactttctgg tatttcatct
    46681 tctaatttta ctcttaaagc atcaatgttt gcgcttatgg gcacagttcc aagtatagtt
    46741 tttgtatact tagtttccat attttcaaga ggaatatcaa agttcaccca tggttctaac
    46801 atacttttat tgttgttaag taagtttatg ttagacctta ttgtacctat ctccttgtgc
    46861 aaattgttta actccagagc ttcagcataa acttgccctt ctttttcact atcaattttg
    46921 ttaaactctc tttttggtgc aagtagggat tttttttgtt tcactagtga agaaacaata
    46981 ttaattgctt ggtttacatg agacattttc tgctctagca ttataacttc agattcttca
    47041 ctatctttta caagataatt ttttaattcc tcctcctcag taagaaaggc actatcatca
    47101 atctgaacaa acccacggtt cataagtagc tttaaaatct ggcttttttg agcttctaag
    47161 ccaacaatag atattttgtt catattaact attgccatat tatctcacta cctttccaat
    47221 aaccgcttca atagccttac tttttctttc ttcagcagtt ttttcaattt tcagacattc
    47281 acttttagca ttagctaaga ttttatcaac ctctgctcta ctgtcggtga ctgccttctc
    47341 aataagagtt tcttccataa gctttgcttg tttttttaca tcagcaaata tgctttcaca
    47401 tttgtttaca gcatcagctc tgatagactc tgcatcagta acagctttta ctttgatact
    47461 gtctgctgtc tgctctgcct caacaatgct ttttattatt tcaaaagcca tccaaatcac
    47521 ctctcttacc tttttactat gtatataacc aattagattt ttttattcta attagattac
    47581 tacctcattg ttaaagtata aacagcattt ttatataata attaatattt ttcaacgttt
    47641 atttcttaat aaaacatatt ttagaatagt acttgcctag atttacatcc ttttttgact
    47701 tacttttaga ctatgacatg agtatggttt ttaaatacca ttattttcaa taattataat
    47761 ttaaagcgat atagtttgtc aatagcgtta ttttgtttta aaattaacgt tatgtcgaca
    47821 ttttaaattt ttattttatt ttttttataa ttttaattta atttattaca tataaaagtt
    47881 ttttatattc tttttcaacc tctaaccatt gaagtttatt gttttttttg aatttttaga
    47941 aatatctcgc actattttat ctaactttta taaatcatca agctatatcg atttttgtca
    48001 ccttattttt aatctaattt ttttatatct agttagaaat aaatcaatat ttttaaagat
    48061 tgataaaact tacacctagt tctaatagtt ttattttaag gcaatgtatt aatcttaagt
    48121 agataatatc attagttact ctatatattc gacaacatta taaaaaaatc ctcgtaccaa
    48181 tcactccaat cttcaatagg atatgctgct cctccattta cacttttcag tagattggat
    48241 gtatattctt ctcccataga aaaaagttcc cctactgtca tatcataacc tctagatgta
    48301 acaaccttac aaaatgaatc cataacatca cgttgttctc ttgtttttat aagttctttt
    48361 ctaaattcat catcattctt agcttcttct aaaattttag ttaatttttc attcattatg
    48421 tatttctcct tttaagtagt ctttctgtaa acttcaataa gctttatata aaaaggaagc
    48481 catcctaaat aaactaagat gacttcgctt aactgttagc aacacattta ctagtataaa
    48541 ataattggtg tacctggttc tatattttca tatattttct ttgccttaga aaaaggtgta
    48601 tttacacatc catgtgaacc tgaagactta tatatacttc caccaaaaga atttctccaa
    48661 gtagcatcat gaatgcctat gttcccatta aacggaagcc aataacttac aggtgaactg
    48721 taaccttgac cagttaatga aacatttcta gccttataat taagaggata tatacctgat
    48781 ggtgtagcaa atcctctagc taaatcacct gttacaacgt ctgttgatac aagcatttct
    48841 ccatttttaa agaaccacat atgttggcca gaaagactaa tctctacata tgtatttcca
    48901 atatcatctt tattacgaga tacagctttt tgtatgtaaa taggttctct tgtaacattt
    48961 ttaccagctt ttacaacatc aactaatgct tcagcttctt tctttttatc tatcaaccaa
    49021 ccataaattc cacctgaaat tgtaatatct ttacctgtgg cagttttaaa tggtcttgag
    49081 tcaccaaaag tactgtattt tttacttaaa cctcttgtat attctaccat agcatcatta
    49141 tcaaaagtta tgtcataatt cttttctgag tctactttta accatttatt aatttcagaa
    49201 ccatctaaag tttccttacg gtcactaaaa tcatatgtaa cgactacacc aacatactta
    49261 tctaatgtct taatagcttg tttcaacttt tctgaatcct ttgtgttgtt tggtgcttca
    49321 taaactttac tttcatccag agaaatagaa gtattcccag aattaattga gttttgtact
    49381 aacttataaa aatcatcttt tttcactttg tttccaagta cctctggtac tataacataa
    49441 gcgtttttag tgctatcata cttaggataa gcactcttag gttctacaac atttttacta
    49501 tcaaaacaag ataaagcatt aaaccttttt tctagtaaat ctttattata tgaaagagtt
    49561 atcatattat tatactcttt agacttaaat attccaccaa tccatccaat agcgctctga
    49621 ctatctttta tcttttgaac tttatcatct ggtgtaaatt ttaaatcaat atcttttgct
    49681 tttatttgtt ctttcttatt gtttctttcc tctaagttta aagtatagga attagtacta
    49741 gatgttaaca aatcatttac ttgctcaact gtttttcctg aagcctttac gccattaata
    49801 gtcgttccaa agtaaaaatg atttctaaaa aagattgcca ttcctatata agctacaaac
    49861 aaaacaccta ctactatagc tatcttaaat tttagatttt tttgtccact ttcttgtttt
    49921 acctcttttc catcaatcat aattaaatcc ttccttacat tgtaattacc tatgtacctt
    49981 attggttttt aaacaaaacc atgtatgcct tattttatat acccaaatta ctgtatcatt
    50041 atttatattt ttaaatatat agttatttct ataaaaccat tttatctttt agataaaatg
    50101 attattataa atcccccctt tcaaaatccc ttaaacacaa tctctgctat attaatatat
    50161 taccatactt ttaataatcg tgggactatc tatctatttt tgtaacacta aactactatt
    50221 ttaaaaaaga aactaatttt gtgttagttt ctttatctta acttattttt atattgaatt
    50281 ttagcttaac ataacgttgt tatatagatt ctatttaatt attttagttt ctatatcttt
    50341 tccatctgca tattctttaa gtaattcctt agcatatcca aaatattctt caatatcaga
    50401 ttcttgacta aaagaaaata tacaaattaa tatttcattt gcatcttctg ttatcattgc
    50461 tctttcagaa tcactagttg gactcccaaa tttacccact gaatcagata atataggaag
    50521 attttctata tttattaatt ctttcccaat acccttatac tgttctcctt catctcctac
    50581 agtaaaacat actggtgaat gtaatttgct tacattatat gtacctactg gatataatga
    50641 ttttattgat attaaattgt ttatttctac tatattattt attgtataaa gacccttccc
    50701 ttgaataatt cttctaacca atgcttctga agatgttcta tatctacttg gattttttcc
    50761 aagctcttta taagcatttc ttgcatcttt aattttatct agtttcgtaa tgtcttcaac
    50821 ttttagactt tgatttatag tatcacaaaa ctcatttatt tcattaatta atttttctga
    50881 gcttttattt actgctactt ttgcttgaat gcaacccagc ttagcattag gccatctttc
    50941 cttaagtttg tcatcaatac ttatatctat catacttaaa caactcctat acactaaatt
    51001 tttattaaag tttttatcat ctatacatta tagtactatt cttttgttaa taacatgttt
    51061 attagcaaaa taaatttatt tttaatataa tttttataaa atacaattat tttttgcttt
    51121 atattagaca atacattgat aaaataaaaa aacactaatt atttagcaat gatttattaa
    51181 aatcaagcat tcataatagt gctttatata aaaatcaaat tttgttttaa agtaaatcta
    51241 aaaaattctt agctggcatt ggtcgtgcaa aataaaatcc ttgaactaaa tcacatccaa
    51301 attgtttcat taactcagct tgttctttac tctcaacacc ttctataatt acaactaaat
    51361 ctagtgattt tgctatattg cgacttctct catcatgttc aatgccacca agaaaagact
    51421 tatctagctt caatatatca attggcatat ctcttaaaca actaatagaa gaatatcctg
    51481 taccaaagtc atccatagaa atagaaaatc catacgtcct caactcattt aaaacattct
    51541 ttaaaatatt taactcatta atagccacag tttctgttat ctctatttca atgaaatctg
    51601 aacttaaatt atacattttt attgttttat tgataaattt tacaatatct ggttgataca
    51661 actcgaccct agataaattt actgaaactg gaactatctt tttaccttgc tttttccatt
    51721 catataaatc attgcaaacc ctttcaaata caaatctccc aatttttaaa atcaacttac
    51781 ttttctctgc tattggaata aatactgcgg gacttataaa accatgttct ggatgattcc
    51841 atcttattag tgcttcactc cctatcattt ctccagttac aagtgaaaac ttaggttgat
    51901 aatacacttc aaattgattt tttaccaatg ctatttttat atcatctaaa atcattgatt
    51961 cctcactaag cttattccta acatcttcat tataaatagc ataatttata tttttacctt
    52021 tagcaacaga cctagccata tttgctttat ttacagcctt ttgaatatct acttcacctt
    52081 cttcaacaaa ataaataccc acacacagag aaatttcaaa gactatactc aaattacttt
    52141 caatattact tcgtatatta tcaagttttc ttataatttt atttcttcca tttttataat
    52201 cacagaaaat tgcaaaataa tcacttgaaa gtcgtgcata tacctcacct tctgttaatt
    52261 cttcttttaa agcttttgct attgtaatta ataaattgtc accaaattca tatccaaaag
    52321 tatcattaat catcttaaag ttattaatat caaaatatac tataatataa tttaaagatg
    52381 gtttatctaa aagatactta ctacattctc tataaaattt attttgattt cctatatatg
    52441 taactttatc ttcataagcc aaacttaata tttctttttc attagatttt tttatataaa
    52501 ttatataaaa aataattgca ataaatatta atacaataga taacactgca tatacagctc
    52561 tgttgattat ttgttttgaa ttactaaata caacagtttc tggaattgaa aaaattaaat
    52621 tccagtcttt tatataatta ttttcactat tcccattatc catagtggca tatcctaaat
    52681 atcttctttc ttccaacata tttaatacag ttgcacctgt tttattttct tgcaattctt
    52741 tttttaatct atttacttct tgaatatcat tatcctgttt taacaattta taaatatttt
    52801 ttgataggct atccccttgt ggatgaagga taattgtacc attatttttt acaacataag
    52861 atatactatt attttcataa aaagatatat taataagttt catcagagta ttgatattgt
    52921 aagtacaata taaaactcca actactttat cattatttaa taatggtact gaaatagcaa
    52981 tcttctttat agacttattt acttcatcaa catatggact tgaaacatac ttcttacctt
    53041 taattgagtt tttgaaaaat tctctatctt tcactgaaaa cttatcacct ttggtatttt
    53101 cagcattacc atttatatca actattgcca tagtgtaaaa tttttgattt tccatttcat
    53161 ctaaaagatt tttcattttt tcctcattag gtatatctgg atttgaagca taatagttag
    53221 aaaaaatatt taaaacatct aaatcttttt caatttgtct ttgtgctagt tgagagcctt
    53281 gtactgcaac ttcttttata tgtatttttg cctctgacat aagaagatgt tttgtatctt
    53341 ctatatagat ataccccatt gtcaaaaata atgatgttaa aaaaagaatt gtaattaatg
    53401 taaaactttt atttctccct gtcattcgac tcctccccta attagtcaat acttcatata
    53461 gataatatat cctttttata ccaattcatc atttatattg tatataacta ttcttaaatg
    53521 ttacacataa aatcaacata ataaccataa aaacattctt ttattattat aataacacta
    53581 tttaattgaa ttttaaatta aagaataaaa actatccatt ttctcaaaca aaaaaactct
    53641 tatacaataa aactttgttt tatgtataag agtttttatt ttatctattt tatagcaaac
    53701 ttatcttcca taatatgaat ttaaatacat atcttttatt tcattcataa gtggatatct
    53761 tggatttgct cctgtacatt ggtcatcaaa tgcttgaatt accatttcat ctattgaatc
    53821 cataaactta gattcttcaa cgccaaactc ttttatagat ttaggaatac caactttaag
    53881 ttttaaatct tcaatagcta ccaataaact ttggaatttc tcttcatcag aatttccttt
    53941 taatcctaaa aatgaagcta tttcagcata tctctttaat gcttctggat atttatattg
    54001 agagaatgct cccattttct ttggtgcatc tgaagaatta aatttcataa cttctgttat
    54061 caaaagagca tttgcaacac catgtggtac atggtggaaa gcacctaatt tatgagccat
    54121 agagtgacaa acacccaaaa atgcatttgc aaaagccata cctgccattg tagaagcatt
    54181 tgccattttt tctctagctt ctgggtcttt tgcaccatta tcatatgcac gtggaagata
    54241 ttcaaatata ctcttaatag cttgtaaagc taatccattt gtataatcgg ttgcaagcat
    54301 agatacatat gcttctaatg catgagttaa agcatctata ccagaagcag atgttaagct
    54361 ttttggcata ttcatcatca tatcagaatc aactatagcc atatttggca tcagttcata
    54421 atctgctaat ggatatttta ctcctgtatc ttggtcagtt ataacagcaa atggtgttac
    54481 ttctgaacca gtacctgctg atgttggtat agctacaaaa ttagcttttt cacccatttt
    54541 agggaatgta tatacccttt ttcttatatc cataaatctc atagctaggt cttggaaatc
    54601 tacatctgga tgctcataca atgtccacat tatctttcct gcatccattg cacttcctcc
    54661 acctatagat attattacat ctggattaaa ttctctcata gctttagcgc ctatcttagc
    54721 acattccaaa gttgggtctg gttctacttc aaagaatgta gtgtacttaa tattcatttc
    54781 atctaataaa tcagtaactg ctttagtata tccattattg taaaggaatg tatctgttac
    54841 aataaaagct ctcttcttat tcattacatc tttaagttct tttaatgcta ctcctaaaca
    54901 accttttttg aaataaactt tttctggtgc tctaaaccaa agcatatttt ctctcctctc
    54961 agctactgtt ttaatattaa gtaaatgctt aactcctaca ttttcagata ctgaatttcc
    55021 accccaagac ccacatccta aagtaagaga aggagctagt ttaaaattat ataagtcccc
    55081 tataccacct tgagaagaag gagtattaat taaaattcta caagttttca tggctgaagt
    55141 aaacttatca agtttttctc tttgatttac atcatcaata tataatgaag atgtatgccc
    55201 aaatccacca tcttctacta atttttctgc ttttctaact gcatcatcaa aatcacttgc
    55261 cttgtacatt gcaagtactg gtgaaagttt ctcatgagca aaagcttctt ctatttcaac
    55321 tgattctact tcacctatta aaaccttagt atctactggg acttcaaagc ctgcaagttt
    55381 tgctatagta caagctgttt gcccaactat tttagaattt aatcctccat ttactaaaat
    55441 tatatttctt attttttctg tttcatcttt atctaataaa taagcaccac gctctttaaa
    55501 ttcttttttg acttcattgt atatattttc taatactata accgattgtt cagaagcaca
    55561 tatcatacca ttgtcaaaag tcttagatac aagaattgag tttacagcag tttttatatc
    55621 tgcactatca tcaattattg ctggagtatt acctgcacca actccaatag ctggttttcc
    55681 agatgaatat gctgatttaa ccattccagg acctcctgta gcaagggtca aatccacttc
    55741 tttcataaca gttgtagtta attctaaaga tggctcatct atccatccta ttataccttc
    55801 aggagctcca gctaaaacag cagcctcaag aacaactttt gcagcctcta tagtagaatt
    55861 ttttgctctt ggatgtggag agaaaattat tccatttcta gtttttaaag ctaaaagtgc
    55921 tttaaagata gcagtagaag ttggattagt tgtaggtaca actgctgcaa taacacctat
    55981 tggttcagca attttagtca taccaaatgc ttcgtctttc tctataacac cacaagtttt
    56041 agtttcttta tatgcattat atatatactc tgaggcataa tgattcttta taaccttatc
    56101 ttcagcaatt cccataccag tttctttttg tgccattata gctagaggaa ctctttgctt
    56161 attagctgcc aaagatgctg ctaaaaaaat tttatctacc tgctcttgac tatatgtagc
    56221 aaatattttt tgagcttctc tgatttttgc tagtttttta actaagcttt caacgttatt
    56281 tacagtatct aaaccaactg acataacttc attttctttt ttattttcta ttattacttg
    56341 ttctttatga gccattttaa caacctcctg agatagtttt ttatttatta tgttaattta
    56401 ttaacattat agcatttact tttcctattt tcaagttatt tttttaacat tt
//
LOCUS       DAMJZE010000005        54928 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJZE010000005 DAMJZE010000000
VERSION     DAMJZE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.005"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            4..984
                     /locus_tag="PC541_000222"
     CDS             4..984
                     /locus_tag="PC541_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2837710.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1083..2045)
                     /locus_tag="PC541_000223"
     CDS             complement(1083..2045)
                     /locus_tag="PC541_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2837711.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2361..3311)
                     /gene="hpdA"
                     /locus_tag="PC541_000224"
     CDS             complement(2361..3311)
                     /gene="hpdA"
                     /locus_tag="PC541_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2837712.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3304..3561)
                     /gene="hpdC"
                     /locus_tag="PC541_000225"
     CDS             complement(3304..3561)
                     /gene="hpdC"
                     /locus_tag="PC541_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2837713.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3565..6273)
                     /gene="hpdB"
                     /locus_tag="PC541_000226"
     CDS             complement(3565..6273)
                     /gene="hpdB"
                     /locus_tag="PC541_000226"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2837714.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6570..7586)
                     /gene="tsaD"
                     /locus_tag="PC541_000227"
     CDS             complement(6570..7586)
                     /gene="tsaD"
                     /locus_tag="PC541_000227"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2837715.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7592..8068)
                     /gene="rimI"
                     /locus_tag="PC541_000228"
     CDS             complement(7592..8068)
                     /gene="rimI"
                     /locus_tag="PC541_000228"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2837716.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8058..8774)
                     /gene="tsaB"
                     /locus_tag="PC541_000229"
     CDS             complement(8058..8774)
                     /gene="tsaB"
                     /locus_tag="PC541_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2837717.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8774..9226)
                     /gene="tsaE"
                     /locus_tag="PC541_000230"
     CDS             complement(8774..9226)
                     /gene="tsaE"
                     /locus_tag="PC541_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2837718.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9365..10405)
                     /locus_tag="PC541_000231"
     CDS             complement(9365..10405)
                     /locus_tag="PC541_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2837719.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(10734..11339)
                     /locus_tag="PC541_000232"
     CDS             complement(10734..11339)
                     /locus_tag="PC541_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2837720.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(11464..11581)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11641..12807)
                     /locus_tag="PC541_000233"
     CDS             complement(11641..12807)
                     /locus_tag="PC541_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2837721.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(12955..15096)
                     /locus_tag="PC541_000234"
     CDS             complement(12955..15096)
                     /locus_tag="PC541_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2837722.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(15240..16347)
                     /gene="prfB"
                     /locus_tag="PC541_000235"
     CDS             complement(join(15240..16271,16273..16347))
                     /gene="prfB"
                     /locus_tag="PC541_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2837723.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(16362..19037)
                     /gene="secA"
                     /locus_tag="PC541_000236"
     CDS             complement(16362..19037)
                     /gene="secA"
                     /locus_tag="PC541_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2837724.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(19169..19720)
                     /gene="raiA"
                     /locus_tag="PC541_000237"
     CDS             complement(19169..19720)
                     /gene="raiA"
                     /locus_tag="PC541_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2837725.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(20273..20947)
                     /locus_tag="PC541_000238"
     CDS             complement(20273..20947)
                     /locus_tag="PC541_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2837726.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(20950..22002)
                     /locus_tag="PC541_000239"
     CDS             complement(20950..22002)
                     /locus_tag="PC541_000239"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2837727.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(22051..23010)
                     /locus_tag="PC541_000240"
     CDS             complement(22051..23010)
                     /locus_tag="PC541_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2837728.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(23103..23624)
                     /locus_tag="PC541_000241"
     CDS             complement(23103..23624)
                     /locus_tag="PC541_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2837729.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(23635..24969)
                     /gene="celB"
                     /locus_tag="PC541_000242"
     CDS             complement(23635..24969)
                     /gene="celB"
                     /locus_tag="PC541_000242"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2837730.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(25008..25334)
                     /locus_tag="PC541_000243"
     CDS             complement(25008..25334)
                     /locus_tag="PC541_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2837731.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(25358..25666)
                     /locus_tag="PC541_000244"
     CDS             complement(25358..25666)
                     /locus_tag="PC541_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2837732.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(25807..27720)
                     /locus_tag="PC541_000245"
     CDS             complement(25807..27720)
                     /locus_tag="PC541_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2837733.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(28052..28834)
                     /locus_tag="PC541_000246"
     CDS             complement(28052..28834)
                     /locus_tag="PC541_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2837734.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(28837..31056)
                     /locus_tag="PC541_000247"
     CDS             complement(28837..31056)
                     /locus_tag="PC541_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2837735.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(31320..32417)
                     /locus_tag="PC541_000248"
     CDS             complement(31320..32417)
                     /locus_tag="PC541_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837736.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(32732..33925)
                     /locus_tag="PC541_000249"
     CDS             complement(32732..33925)
                     /locus_tag="PC541_000249"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2837737.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(34062..34165)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            34354..34863
                     /gene="yyaC"
                     /locus_tag="PC541_000250"
     CDS             34354..34863
                     /gene="yyaC"
                     /locus_tag="PC541_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2837738.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(34974..35402)
                     /gene="fabZ"
                     /locus_tag="PC541_000251"
     CDS             complement(34974..35402)
                     /gene="fabZ"
                     /locus_tag="PC541_000251"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2837739.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(35418..36437)
                     /locus_tag="PC541_000252"
     CDS             complement(35418..36437)
                     /locus_tag="PC541_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2837740.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(36634..36894)
                     /gene="spoIIID"
                     /locus_tag="PC541_000253"
     CDS             complement(36634..36894)
                     /gene="spoIIID"
                     /locus_tag="PC541_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2837741.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(36992..37660)
                     /locus_tag="PC541_000254"
     CDS             complement(36992..37660)
                     /locus_tag="PC541_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2837742.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(37726..38790)
                     /gene="spoIID"
                     /locus_tag="PC541_000255"
     CDS             complement(37726..38790)
                     /gene="spoIID"
                     /locus_tag="PC541_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2837743.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(38877..40130)
                     /gene="murA"
                     /locus_tag="PC541_000256"
     CDS             complement(38877..40130)
                     /gene="murA"
                     /locus_tag="PC541_000256"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2837744.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(40151..40888)
                     /locus_tag="PC541_000257"
     CDS             complement(40151..40888)
                     /locus_tag="PC541_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2837745.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(41344..42639)
                     /locus_tag="PC541_000258"
     CDS             complement(41344..42639)
                     /locus_tag="PC541_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2837746.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(43090..44922)
                     /gene="glmS"
                     /locus_tag="PC541_000259"
     CDS             complement(43090..44922)
                     /gene="glmS"
                     /locus_tag="PC541_000259"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2837747.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(45189..46535)
                     /locus_tag="PC541_000260"
     CDS             complement(45189..46535)
                     /locus_tag="PC541_000260"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2837748.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(46705..47262)
                     /locus_tag="PC541_000261"
     CDS             complement(46705..47262)
                     /locus_tag="PC541_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2837749.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(47263..48015)
                     /locus_tag="PC541_000262"
     CDS             complement(47263..48015)
                     /locus_tag="PC541_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2837750.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(48015..49094)
                     /locus_tag="PC541_000263"
     CDS             complement(48015..49094)
                     /locus_tag="PC541_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2837751.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(49123..49338)
                     /locus_tag="PC541_000264"
     CDS             complement(49123..49338)
                     /locus_tag="PC541_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2837752.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(49381..50058)
                     /locus_tag="PC541_000265"
     CDS             complement(49381..50058)
                     /locus_tag="PC541_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2837753.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(50048..51127)
                     /gene="buk"
                     /locus_tag="PC541_000266"
     CDS             complement(50048..51127)
                     /gene="buk"
                     /locus_tag="PC541_000266"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2837754.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(51149..52051)
                     /gene="ptb"
                     /locus_tag="PC541_000267"
     CDS             complement(51149..52051)
                     /gene="ptb"
                     /locus_tag="PC541_000267"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2837755.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(52355..53536)
                     /locus_tag="PC541_000268"
     CDS             complement(52355..53536)
                     /locus_tag="PC541_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837756.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(53533..54372)
                     /locus_tag="PC541_000269"
     CDS             complement(53533..54372)
                     /locus_tag="PC541_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2837757.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        16601 a        10364 c         5813 g        22150 t
ORIGIN      
        1 ttaatgaact taaaattaat atggataaat attctaaacc cagtatttat taaaaatgaa
       61 ataaaaagaa aaaaaataca aattattatc tttttcttta taaactttgg tttaacttat
      121 tcattaggtt tactgttata ttataataaa tttattgatc cagaaaactt tgcatcattc
      181 atgatgatat tacctctatc ttcagttgcc attgcaaagt tttatactga aggattaaat
      241 aatgatatgt atgaatttta ttcactaatc atattatttt ttcttattta ttcattatta
      301 attgttctta agttattaaa tctaataaat aataatgaat ttataattat aaacctaata
      361 ttaactttta taagtagctt atcaattata atatattcat caaataaaaa aaatttatat
      421 cctgtaaaaa atattaaact aggttcttta ttaattattt actttatgat ttctgtaatt
      481 gttccaacat catttatact aatactacaa ggggctcaac ctaattatag tggtatcata
      541 aaatttatat ttactccaat aacatcatta actactattt atattttttt tagtgaagaa
      601 tatgcctgga gaggtttttt acagaatatt ttttttgata aatttggtaa aaagttagga
      661 gttataatat taggtatgtg ttggagcttg tggcacttac cactaatatt tacattatat
      721 acacctgaag cgccaatatt aggaataata ttacgttcaa tacatatagt tggaatttca
      781 atatttttag gatatcttta tataaaaact aagaatatct ggttttgtgc tataattcat
      841 gttttaaata atagtgcttt tcttatcaca aattctaaag aatcaactat cacttaccat
      901 gatatactaa tagtatctat ggttgtttta attttttatg ttccatttct atttacaaaa
      961 gaatataaaa ataacttgga gtaaatagaa tatccaagtt atttttatat ttttgcaaat
     1021 ttccaacaat cctacagaat actttaagct tagaagatat tattaaaatt tattataatc
     1081 aactaattct tgatattggt cacatttact ttatcactaa caaaatcata cttagaatct
     1141 ctatagtaat accatacaaa tataaagcta agtagattaa caaaagtagt caaaattcca
     1201 ccttcaatac caaacattcc tccatttaat agattaccat gagtgatttg tgtagttata
     1261 atccctggaa cttgaagtcc acttacaggc ataccatata taatcccctg aaatacattc
     1321 catgcaaaat gataacctat acacatccat atattcgacg attttataaa catatatgaa
     1381 aaaagaatcc caataattat aatattaatt acagacaaaa aagtaacatc aggattccat
     1441 atatgaatca acccaaaaat aacagatgga acaatcatta taaaataaat atttttacat
     1501 cttcgtaaaa ctgccataaa aaaaccacga tttaaaattt cttctgcaaa agcaactaaa
     1561 ataaagacca gtatccaaga aactgttaaa ggtgaaattt ttggtaccca tgaagtaaca
     1621 tgaccaccac caattgttat tgttataata aagattactg tacaattaaa tattcctaat
     1681 aacattccta catatccttc ttttttctta attctataag aatgtaatcc catttcacta
     1741 ataggatttt tcataataaa tctccataca attataggaa taataatcat aagtgaatct
     1801 gttagaattt gaaatacaat ctgtaaccca acatcattta accaattagc atattctgaa
     1861 aaatagtttg tttttaaatt aatgtcacct gtagcaacca ggatttttct tattatttcc
     1921 acaagtatca tgcttaaata atactgaagt atgtaaaagg tagccataac agctataata
     1981 atccaccctg aacgtacttt tttgtcttta ttcttaataa tcaagttgct ccatgatttg
     2041 ttcatataga tgtcttcctc ctaactatta tttttagttg tttctaaaat atattctatt
     2101 aaattttttc atagtagtat tttaccacaa aacttctgct tcaatgaagt aagcgtaata
     2161 tattctctaa ctgtaattta aactataaaa aatatataaa gtatatttaa agcaatattt
     2221 atggacataa agcaatataa acaatatata aaacatataa aactttactt aattatttct
     2281 acataaaaac cacataaaaa aggatgtaac tctagctttt agtgtaacat ccttttttta
     2341 attaaaactt gtatttaatt ttagaaagct gtctcatgac caacataaca agctatacca
     2401 ttatccaaaa atatatcttg taattcttca agatgacctt catcaacatc acccttatca
     2461 gaatactcat attccttgcc taattgaatc cattttgatt ctcctaatct gtgaaatggt
     2521 aatagattaa tttctaccaa attatttttg tgcataaaag atataatatc acttatattt
     2581 tcaacactat cattaaaccc agagataact ggtactctaa gtactaatct tccattccaa
     2641 tcagaatttg caaggtttga tatattttct aatattaaat cattatacac acctgtttgt
     2701 tctttgtgtt tttctctatc catatgcttt atatcaataa atgcaaaatc aatatcgtta
     2761 aatattttat taaaaacttc atttgataca catgcactcg tttcaatggc agtgtgtata
     2821 tttacctcat gacactttaa taatacttca tgtaaaaatt catgctgaag tagtggctca
     2881 cctccactaa atgttactcc tccattactt ctccaattgt tagaatcacg tttaattacc
     2941 tgcacaagtt catctacagt gtaaggtttt gcacataatt taaacgcatt ataataacat
     3001 gagttgacgc attcaaaact ttcacagtcc ttacagatgt tccaatctat aactggctta
     3061 ttatcaagat taaagctcaa tgcaccattt ttacacttac catgacatac agtacatcca
     3121 ttttcatatt gacaagataa ttcactaaac atcatatgtg gtcttacagt ccaactttct
     3181 ggatttgcac accatttaca actcagtgga catccattta aaaatacagt tgttctacaa
     3241 cctggaccat catgaactga aaagctttgt acatcaaaaa tcatgccttc taattgcttt
     3301 tgactactca ttaaacacat gtccttcaca agtaattgca ttcaatgttg aatataccca
     3361 gtcttctttc tctaggccaa cacatttgcc tataccttca tcattagctt caacaaaatt
     3421 tttacaattt ttacattttg gcattacttt tatctctgga catgctgcat catctaaatt
     3481 aatcatttgt tttgataatc tacaaattcc tttggttgca tctactgcac aaaaattcat
     3541 acaatcacta tgctttctca tattttacac cccttcatac tctgttctag caattacttc
     3601 atcttgtatt ggtttaccta attcacacca atattgagta aacccagcaa cacgtaccat
     3661 taattgacga taattgtcag gatttttttg tgcatctttt aatgtttctg aatctacaac
     3721 attatattga atatggaacc caccttttct taaataactt cttgttaaat ctaataattt
     3781 tttagttcct tgttgtcctt ttatagtagt tggatgaagt tttaaattca tctgagaatt
     3841 ttgaactact gcttggtccc aaatagttgc agattcaaat aatgcatata ctccattttt
     3901 atctgttcct gcatatgcag agactgaacc atctgaatat gttgttccac ttagtctacc
     3961 atctggagta gccaaggtag cagctccctg tggcccatgt gtagaaacag atatttggca
     4021 agggtacatc ttcttagcat ataaagattg tgcttcttca catacttttg ataaccatac
     4081 ctcatagttt ttaagaatat tatctgcata caaatcatta tttccatatt taggtgcatc
     4141 aagacaggcc ttataaatag catcatattt accagtctta tctactttta cttggtctgc
     4201 catagaataa tttcctactt ctaatgcatc tttaaatcca aaattattta atatggcatc
     4261 cttcatttcc tctattgtaa atgctttgtc atcatataca agtttcttaa gtgctgcaaa
     4321 tgaattgacc atagtaacag taccacaagt ttctacgttt aatgttgcat tataacgata
     4381 tcccatattt cctatatgtt gacctttgtc taaacagtct ggctttagta gtgaatttat
     4441 tatagaagta tcaaattttc tccatatatc taactcaatg ttatttgctc tttctaaaac
     4501 atttattgct tgtttataat aatctttaaa tacttctatg acttcttcat aagtatctaa
     4561 tggcttatta tgaggctcaa atacttgtat attcatacga tagtctttac cattcataag
     4621 aacaagttct aaaatttttg gattagctat aaagtgaact ccagagccag cagattgacc
     4681 tgctccacct gcaatagagt atgtttttcc gtttaaagtt aactgtttcc aacaccctgg
     4741 agacgtttcc aaacatccac ctaatgcaaa tgctcttgct tcttcaacag tcataccttc
     4801 atcagcaaat tgtttcatca taaatgttgt tccatttgag ttgtttaccc atgctggata
     4861 accagaacca agctttgtac attcagcagc tttcattaag aaatcttctg gaagcttttc
     4921 atcatatagc atagttaaac taggttgagg agttctacat ctcatacttg cttctaataa
     4981 taactcttct agttcattag ctcctgtaga accatcttct tttaatccac ctatagataa
     5041 agtattaaat gtattgcctg atagtactcc tccatttacg cccgctgaag caaaacagtc
     5101 aatacaagtg atttttattc tataaagctc taataattcg ataacctctt cttttgtaat
     5161 tagtcctttt tctatatctt gttcatacca aggatataaa atttgaccaa aacgtcctat
     5221 agacatacca gatatagcat cctcatttac tgatgctata tgaattgtat aagtcaactg
     5281 aagagcttcc ctaaatgtac gaggttgttt atgtgcaata tgttctaata tctccactaa
     5341 atctgagtat tcttttttag cttctaaatc tgtctctata ctttctaaat attttgcatg
     5401 ttttgcataa tttaaaatcc aagtttgtaa tccttcaata acttgaataa cagcctcata
     5461 aaaatataga cggtccatac caataagccc atctccagat gcatttcctg caactgcctt
     5521 tttacattct tttgccattt ctataattcc atctaatcca taatttaaag gataaaaata
     5581 atttataact tctctacctt gaggaagtgt ataccctgaa tcaaacatgc atataagtgt
     5641 actcattagg ttttctttta aatcataatc tggcatcatt ttttcatagt gaaaacctaa
     5701 atcttcaaca gactttccaa cccattcctt tgccattttt acaagtacag gtacttcttc
     5761 ttttctcatt ccgaatttac cagctattga aacgacattt ccaaaacttt cagtaacatt
     5821 tcctccacca gacccaaatt ttgtaagttc atcagctgtg ttactggcta atttctttgc
     5881 ctcttctctc atttgttctc cttgagcaac aaagaagctt tcacttaccc atggcattgg
     5941 aaaagaacct ctataatggc gtgtcttttg cattactatt ttctcaccag gtattatatt
     6001 tggtgtcata tgtgaaaaag cagctttaag agctttagct cttctaacta ctggtatatc
     6061 accatctgat tttctatatt ctctattata ccaatatgga aattccatat cagcagtaga
     6121 taatgtatta taatatacat ctaataagtc ttctgctctt ttacttgctt ttttttcatt
     6181 aaattcactt aaattttctt tattttgaaa atttgattta atattttttg cttccaaaat
     6241 acttcttatt ttgtcttctt tactttgact catttcttcc cctccttaat ctttctataa
     6301 ttagtataaa actttttaag aatgaaaaaa aagggtatat tacccacttt ttaaaataat
     6361 ctttctatag aaaaaaataa acttgaagca aatctaagcc aaagacaatc tgtatatcct
     6421 aaaatttgtt gcaattcatt ggaatataaa gattataaat agttttactt caagttttaa
     6481 tatatactat atttattttt attatattta atttcacttc tacttacttt tttgaaacaa
     6541 tacttttata tttacaaatt catctaactc tactgattta tctttaaatt tggaactgca
     6601 tttaaactca tatcatgcgt tttcccattt ataaaattat aatatcctgc acaccctatc
     6661 atagctgcat tatctgtgca taaaattaga ggaggatact taacagtaat accattgtct
     6721 ttagcaagct ctgttatttt agctctaagc cccgaattag aagcaacccc accagaaaga
     6781 gttatgatat tgtatccttt gtcttttact gcttttagtg ctttagtaga taaaacctct
     6841 actacagcct cttggaaaga tgctgctaca tcttcaacta ctatttcttc atttttcatt
     6901 ctttttccat ttagataatt taatacagaa gattttaatc cactaaaact aaaatcatag
     6961 ctatcttctt ctaaataggc tcttggaaat tctatagcat gtttatttcc attcttggcc
     7021 agattatcaa taataggacc tcctggatag cctaaattca tagctcttga aatcttatca
     7081 aatgcttctc cagatgcatc atctcttgtt tttcctaaaa tttcatactt gccataatct
     7141 tttacttcaa ccaagtgagt atgacctcca gatactatta atgttataaa aggaggtttt
     7201 aaatccttat gctctatata gtttgcactt agatgccctt ctatatgatt tactcctaca
     7261 agtggaatat tcaaagtata tgcaagtgct ttagcataag atagaccaac caataaagca
     7321 cctactagcc caggtccata tgtaactgcg atatggtcta tatcattaaa acctatattt
     7381 gctttgtcta aagcttcttg aacaactata tcaatatttt ccacatgttt tcttgaggcc
     7441 acttctggaa ctacaccacc aaatttttta tgtgtctcga tttgagtaga gattatgttt
     7501 gaaagtactt ctctaccatt ttttaaaact gatgcagctg tttcgtcaca actactctct
     7561 atagctaatg ttattatatc actcataaac tctacacctc ttttaattgg ttccacatta
     7621 taattgcatc ttctttatta tcactgtagt attcttttct tattccaccc attttaaatc
     7681 catattttct atataaattt tgtgctattt tatttgatgc tctaacttct aatgtcatag
     7741 atgcaatatt attatcttta cataaatcaa ctatgccttt tatcaacttg tcccctattt
     7801 ttttccctct atagtcacta tgaacagcca cattatttat atgcccttca tcaagtataa
     7861 gccaaattcc cacatatccg acaatttttc catttaactt agctactata taccttgcta
     7921 cttcattaga caactctttt ctaaaagact cttttgacca ataatcttca aaacaatttt
     7981 tttctacttc aaaaacttca tctatatctt ctgtagtcat ttcttctatt tttatatcat
     8041 ctactattag ttttgtatta tcttccatta tttagcctct tcattttttc atcatattgt
     8101 acttcagctt gagattttct gatatacata ggatttatcg tataacaagt atgtacatct
     8161 atatttttat tatatttatt taatgctaaa gagcataagc ttcctgcgtt agatacatta
     8221 ttagctggtg aaggtatttt tatatttttt atattctcta acttgtcttt atatttgtat
     8281 acagcttctc caactattat aaattcttca ttagttgata caatttcatc tactaaagca
     8341 tcaaactcta caacatcaac accatctaat tctatcatct ctccattttc aaacttgtat
     8401 ttagctgaat aaacttgatt tttctgtgca tctaatatac aacatatctt tctatcacag
     8461 aaatttatgt tatttgctag agattccaaa gaatttactg ctattattgg tatattattt
     8521 acatgggcca tagcttttac tgtagccata cctattctaa gtcctgtaaa cgaacctgga
     8581 cctatacata cagctaataa atccatatct tttatactta aatcactcat actcaacata
     8641 ttttctatca ttggcataag cttttgtgaa tgagtttttt tgttgtttac tgtaaactca
     8701 catattaagt tatcatcttc aacaactgct acacttgctg ccatagaaga cgtatccata
     8761 cctagtattt tcattattta atcaactcct caactatctc tttataaaac tctccttttg
     8821 gagttaatat catttctctt ccttcatctt tatatctcaa ttctatattc aaatattctt
     8881 ttggaagtat atcttctatt agattagccc attctattat acagattcca tttgaattta
     8941 catattcatc ataaccaata tcatacattt catcactact acctattcta taaacatcaa
     9001 aatgatacaa aggtattttt ccttcatatt catttattat agtaaaagta ggacttgtta
     9061 tataatcttc tatccctaat gaatctgcca aactttgtgt cattgtagtt ttaccagctc
     9121 ctaaatctcc tactaaacag ataacactgc cttcttttaa aagtttacct aatttatatc
     9181 ctatttctct agttttattt tcattttcta aatatatttt tgccatcaat tcatctccat
     9241 tttgatataa tcgtcatgtg aatttatatc actgcttaat taattatagt ataagcctag
     9301 attattataa agtacttgtc tttatctttt tttatctttc actattgatg gctgtatcta
     9361 caccttatat acaatcatca atagcaattt ttacatactc ttttgcttta tggttttgct
     9421 tcaatgcttc tttacatatt ctctcagatt gctcttttac atacttaaga gacttatagt
     9481 tctcttttac tgcttcaata cacatttttt ctgtttgatt ttttatatac ataagcattt
     9541 ttccattttg tcttacaatc tctatacata tatcttctgt atacttatct atgtaactta
     9601 gtacatcttt aatattatat tttttattta aatatcttat agcatttata catatttctt
     9661 ctgtcttatt gttgacaaat tgtatacttc ttccatcttg tttgacagct tctatacata
     9721 ttttatcagt ttgtttattt acatattgta atgccatacc attttgtctt acggattcta
     9781 tacacatttt ttccgtttga ttttttacat attttaatgc tttataattt tgcttaatgg
     9841 cctctataca taattcttca ctctgttctc taacgtatgc aagacacctg ccatcttttt
     9901 taattgcttc taaacatatc cttcttgttt gagtctctaa agcctttagt atacttacaa
     9961 catctatatt ggaagaatga cttattgctt ttataataag cccttctgtt ttatttcgta
    10021 catacttaag tgcattacaa tcttttttta tagcttcaag acacaagtat tctgtttgtt
    10081 cttttacata ttcaagtgct ttataattat actttattgc ttctaaacat aattcttcgg
    10141 tttgattttt tacatactct aatgccttgt aattctgcct tattgctaaa atacacatac
    10201 catcagtttg attttctata ttttttaatt cacgataatc gtattttacc ctttccatat
    10261 tttcaatata ttcattagtg atatacttta aagaatctcc taattttgta tcataaatac
    10321 caatatgaat atctttccaa ttactattaa aataataccc tttatcatct aaaaataatt
    10381 tttttatttt tttagataaa tgcatgaata tacaccccta tcattattaa attagttatt
    10441 attaaaagtt tgattattaa tctttatagt tttttaataa taactttatt ttttgtttat
    10501 tataaattcg ctctgtatat tgtagatacc tttttcatat tttaatagtt aatcgtttta
    10561 attcttcctt tattttaaaa tttaaatttg tctaaatatt tcttatttta tatacatttt
    10621 ttgataaatt aataaagcca taatatattt aatatatcaa tcacaatata ggactgaagt
    10681 tataaataaa ttatggctat tcaatacatt taattttata ttttcttgtc taattatttc
    10741 tttattatat ttcccatttt tttataaagt ggcaatgtta ctaaagatat tataacagcc
    10801 ttcagcacat taaatggagc tatcatccaa attacaaaag tgaataaatc atgaacttga
    10861 ggatttatag ctgaacctaa acctattata gcatctagtc ccataagttg accataaagt
    10921 ggcaacatta caaaataatt tactaatgaa ccaattatag tcatagatat tgttcctaat
    10981 acaaaaccaa tcagtacacc ttttaaatct ccacttctct tatatgaata acaaagtatc
    11041 attacataag aaccaccaat taatatattt gcaaattctc ctattcctcc agttgttgtt
    11101 gctgttattg cttgtaacac attttttact acaactattc caaatccaac taatggtcct
    11161 agagacattc ctccgaatat tgctggaata tctgataagt caatttttaa gaaatctgga
    11221 aatatacctg gtattggtgc ttgaataaac attaataaat aagatattgc agataaaatt
    11281 gacattacta ctaaagttct tgttgaaatc atattgcctc tttttgctgt gttttgcata
    11341 attattttcc ccctaatcaa tatgtttata ttttttagga aaaacaaaaa actcgaagac
    11401 cctatcttcg agtttttatg gatatagttt aaacaaacta aaataaactt atttatcttc
    11461 cataaatatc ttctctcatc cagactttac tgtcggcttt ggaatctcac caaatcaacc
    11521 acctaagtag ttcgcgggct ataccgccgg tagggaattt caccctgccc cgaagatctt
    11581 ctttgatttt ttaattttcc ttacactctt atattagtta attttttaat aattgtcaag
    11641 ttattttttc tcatacacta ctttgccatt tattatagtg taaagtacat tactttgaat
    11701 ctctagtgga gaattatccc aaattactag gtctgcatct ttaccaactt ctatagagcc
    11761 tactctatct tctattccta aagccttagc tggatttatc gtaattgatt ctattgcttt
    11821 ttcttctttc ataccatgtt ttactgcaat acctgcacat attggaagat attgaactgg
    11881 tattactgga tggtctgtca ttatacatac atcaagacct gcatttgaaa gtattcctgc
    11941 tgtattaaat gtcaaatttc taagctcaaa tttagacctt tctgacaaag atggccctac
    12001 tattacagga aacccttctt ccactaattc atctacaata agatgacctt ctgtacaatg
    12061 gtctaatgta agctttaaat taaattcttt tgcaattctt attgctgtaa acatatcatc
    12121 tgctctatga gcatgtgcct taaaaggaat ttctcttctt aaaactggta ttaaactttc
    12181 catttttata tcatattcag gtttttcaca gtcttcatca tcatgactct catacatatc
    12241 aatatcctct aaatattctt ctgctttttt taggttttct ctaagtaaag cagctatagc
    12301 cattctagtt tgaggagatt tatcatcctg accataacag ctctttggat tttctccaaa
    12361 agcaatttta gatgcaactg gattttttat taccatttta tcaattcttt ttccacatgt
    12421 ctttatagct atacattgac cacccataac atttgcactt ccaggggttg tacaaacaga
    12481 agttattcca ccttcaaaag cttctttaaa agttctatcc attggattaa ttccatctat
    12541 aggatttaat tgtggtgtta ttgggtcagt ctcttcattt ccatctgctc cttcaaaccc
    12601 tataccatct tcccataaac ctaagtgagt atgagcatct ataaatccag gaaacacaag
    12661 tttattacta gcatctatta cctctacatc taaaggagct atcaaatctt ctccaatttc
    12721 tattattttt ccttcatcta taagtatatc tccatgaatt atcccatttg ttatggtatt
    12781 tattttacca ttttttataa aaatcattca ttttcctcct gattttcaag actatcgttc
    12841 ttattttaat atattttctt actatatact tttacaaatt tttaacattt atacactcga
    12901 gtaatcttat ttacaaatat agaatctaag tactataacc atacataaac ttatctattt
    12961 tttcattgat aaggcaactc tttttttgtt taagtcaatt ccaatgactt ttacatctac
    13021 tatatcacct actgtaacaa tactcatagg gtctttcaca aaactattac tcatttctga
    13081 tttatgaact aatccgtcat tttttattcc aatatctaca aatgcaccaa agtctacaac
    13141 atttcttatt gtacctttta aagtcatacc ctcttgtata tcttcaattt ttaagacatc
    13201 agttctaagt ataggtttta taccttcttc tcttgggtct cttcctggtt ttttgatttc
    13261 agctatgata tcttttaacg taacttgtcc tacttccagt ttttcactta attccctcaa
    13321 gccaatttct tttatttttt catctatttc acctatattc ttattcttta catcatctaa
    13381 agaatatcca attatctcaa tcattttttt acatatatca taactttcag gatgcacacc
    13441 tgtattatct agtggatttt taccctctaa gattctcata aatccagcac attgagtaaa
    13501 tgcttgaggt cctagtcttt ttactttttt caattgtgct cttgacgtaa aatctccatt
    13561 ttcttctcta tatgctatta tattttttgc tatagcttta cttattcctg caacatgttc
    13621 tagtagggag tatgatgcag tatttaaatc tactccaaca ctatttacag aatcttcaac
    13681 aactccatct agtacttctt ctaatctctt tttgtttaaa tcatgctgat attgaccaac
    13741 accaatactt ttagggtcta tttttacaag ctctgctaat gggtcttgta atcttctagc
    13801 tatagatata gctcctctta ttgatacatt tatatctgga tgttcctcat ttgcaagttc
    13861 agatgctgaa taaacagatg ctcctgcttc actaactatc acatactgca cttcgctatc
    13921 tatttctttt atcatttctg atacaaaagt ttctgattct ctagatgcag taccatttcc
    13981 aattgaaatt atatctatat cgtatttttc aatcaatcct tttaaaactt tctttgctcc
    14041 ctctacatca ttttgagggt ctgttggata tactgttgta taatctagta gtttaccatt
    14101 tttatctact acagctattt tacatccagt tctaaatgca gggtcaaatc ccataactac
    14161 tttgtcttta actggtggtt gaagcaataa acttttaaca ttctttccaa atacacttat
    14221 agctctttct tgagctattt ctgttaaatg atttcttatt tctctttcta ttgatgggaa
    14281 aattaatctt ttataagaat cttctattga acttacgatt tcttctttat ttttgaaatt
    14341 tttatcattt acatattcgt ttattatgta atttaaaact ttatcattat ttatttccaa
    14401 cttaaccttt aggaagcttt ctttttctcc tctgtttatt gccaaaactc tatgtggagc
    14461 catacttttt actgcttcgc tataatcata atacatatca taaaccgatt tctcatcagt
    14521 agcacttttg ctaactatca taccttctct taaagctaat tctcttatat actttctaat
    14581 cttagcatca tctgagacta gttctgcaat tatatctcta gcacctttta gagcatcttc
    14641 aatagaaaga acttctttct cttcatcaag ataattcttt gcctctattt ctatattatc
    14701 taaattattc tctagtattg acaatgctaa attttctaat cctttttctt ttgctattgt
    14761 tgctctagtt cttttctttt gtttatatgg agcatatata tcttctactt cttgtaatgt
    14821 ttttgctttt tctatatttt gtgttatttc atctgttaat ttcccttgtt catctattag
    14881 tctaactaca tcatcttttc tactttgaag atttctcaaa tacattaatt tttcataaaa
    14941 ttctcttagt gtaacatcac tcatttcgcc agtcatttct tttctatatc tagctataaa
    15001 tggtattgta tttccttcat caataagttt taatgtatta tttatttgtt catctctaag
    15061 attaaactct ttttttaaga tttgatttat atccaattta ttcactccta atgttagttt
    15121 ttcataagta taacaaaaca gaacgaaggc tgagggttat cgtcctcagc ctaagtgctg
    15181 taagtgcata ttaagcacta accttcttat acatatatct taattacagt gcatatttat
    15241 taaactattt tattcatttt taagtattca ttaataaaca agtctatatt tccattcatt
    15301 acactatcta catttccaaa ctcagcattt gttctatggt ctttaaccaa tttatatggt
    15361 tgaaaaacat atgacctaat ttgactaccc caagttattt gtgagtattt gccttgtata
    15421 tcttctattt tttccttttg ctctaactct tttaattcta tcaatttcgc cattaaaagt
    15481 ctcatagctc tgtccttatt tagatgctga gacctttcat tttggcactg aactactaca
    15541 ccagtaggga tatgtgttat tctaactgct gaatcagtcg tatttacatg ttgccctcca
    15601 gctccagaag ctctatatgt gtctatcttt aaatctgatg gattaatttc tacttctata
    15661 ttttcatcta actctggtgt tacatctata gatgcaaatg aagtatgtct ttttcctgat
    15721 gggtcaaatg gagatattct cactaatcta tgaactcctt tttcactctt taaataccca
    15781 taagcatttg ttccttctac aagaattgta gctgtcttta tccctgcttc agggtctgat
    15841 attatatcta aaagcttaac tttataaccc ttactttctg accatcttat atacattctc
    15901 aaaagcattt gagcccaatc ttgagcatct aagccaccag taccagcatt tatagataat
    15961 atagcattat ttttatcata ctctccattt agaagagtct ttattttcat ttcagataat
    16021 ttttcttcca ttgattctat ggacttttct atatctcttt ctacagaatc atcatcttct
    16081 tctaaaccta tttcaattaa tacttctata tcttctaata agctttttaa gctttcatat
    16141 tcttctaatg tctcttttaa agatttattt tcttgaagta ctctttgagc gacttcatta
    16201 tcattccaaa aatcttgttg attcatcttt tcttcattaa tttgtatatt tttcatcaat
    16261 gaagctatgt caaagagaaa ccctcaattc ttctaaattt gaagccatct gctccatact
    16321 atgtctatac tcttgtaact ttaacattta atcctccgag attatcttcc acaacagttt
    16381 ttatattttt taccacttcc acaagggcat aagtcatttc ttccaacctt ttcatctttt
    16441 ttaacagttt tacttgttgg aagtgttcca tctgatggag aagatagatt ttcaacatct
    16501 acaactgctt ttctttccac tggagtttct attgttatat taaataaata tcttacagta
    16561 tcttctttga tatgtttatt catttcatca aacatatcaa aaccttccat tttataagca
    16621 ataactgggt cttgctgacc tacagcacga agaccaatac cttggcgtaa ttggtccata
    16681 gcatctatat ggtctatcca atggttatca acagcttgaa gtaagattac tctctcaact
    16741 tctctcattc tctcatagcc aacttgttct tcttttgaag tatatatctt catagctatt
    16801 tcataaactt tttctgttat ttcaactgga ttaagttttt ctatttctgg tatttctatg
    16861 ctaccttttg gcaagaacag attatacata tgttctttga atccttcttc atcaaatcct
    16921 ttatcttgtg tataaagagt aactgcttct tctattatag aatgagtcat tgattgtatt
    16981 tgttcttgta aatcttcacc ttctaataca cgttttctct ctgcatatat tatttctctt
    17041 tgtttattca taacatcatc atattgaagt acatgctttc ttattccaaa gtttttacct
    17101 tcgactttct tttgtgctcc ttcaatagac ttagataata ttctatgttc tatcggcatg
    17161 tcttcttcaa gacctatttt atctacaata ccagatattc tatcactacc aaacaatctc
    17221 ataaggtcat catcaagacc tatatagaat cttgaactcc ctgggtcacc ttgacgtcca
    17281 gcacgacctc tcaactggtt atctattctt cgagattcgt gtctttctgt acctattata
    17341 gcaagtccac ctgcttctac aacttgtttt tgttcttctt gtgtttgttg cttaaacttt
    17401 tcaaatagtt tttcatactc ttctctagct tcaaatagaa tttcattacc ttttacaggt
    17461 ataccttcta ttggagtatc tactctattt actatttctt ctttaaatcc atttcttctc
    17521 atttctctct tagttaaaaa gtctggattt ccacctagaa ctatatctgt accacgacca
    17581 gccatattag tagctattgt aacagctcca agtctaccag cttgtgctat tatttctgcc
    17641 tctttatcat gatgcttagc atttaatacc tcgtgcttta tgcccttttt ctttagtatt
    17701 tgtgataata attctgattt ttctattgat actgtaccta caagtattgg ttgattgact
    17761 ttgtgccttt caattatttc ttgtgctaca gcattaaatt tacctatttc acttttgtat
    17821 acacagtctg gtaagtcttc ccttatcata agcttattag taggtacttg gaaaacatcc
    17881 attttgtaga ttgctttaaa ctcttcttct tctgtttttg ctgtaccagt catcccagaa
    17941 agttttttgt acattctaaa gtaattttgg aatgttacag tagctagtgt tttagattct
    18001 ctttgtattt taagaccttc tttagcctct attgcttgat gtaaaccttc tgaatatctt
    18061 ctaccaaaca taagtctacc tgtgaactca tctactatta ctatttctcc atctttagca
    18121 acataatcaa catctttctt cattattaca tgtgctctta aagcttgatt tatatgatgg
    18181 tataattctg tatgagatat atctgttata ttatcaacat taaaatacac ttctgctttt
    18241 tgtatacccg atgctgttag agatacagcc ttatcttttt cttctaattc ataatcatct
    18301 ggttttaagg ttaaaacaaa tgtgtttgca tctgaatata agtgagttga tttgtctcca
    18361 ggtccagata tgataagtgg tgttctagcc tcatctatta gtatagagtc aacctcatcc
    18421 actatagcat aatttaatcc tctttgaact ctttgctctt tgtgtattac catattgtcc
    18481 tttagatagt caaatccata ttcattattt gtaccataag ttatatcaca atcatattgt
    18541 tcttttctaa cttttggatt ttgaccgtgt ataataacgc ctacactcat tcctaaaaac
    18601 tcatagattt ttgccatttg gtctctatca cgttttgcaa ggtaatcatt tactgttaca
    18661 acatgtacac cttttccagt aagcgcattc aaataaacag gagcagttgc aaccaaagtt
    18721 ttaccttcac ctgttttcat ttctgctatt cttccttgat gtagtactat acctccaatc
    18781 atttgaactc tgtagtgtct aagtcctaac actctcttag aaacttctct aactactgca
    18841 aaagcttctg gaagtatatc atctatactt tcaccatttg ctaatctttc tttaaatata
    18901 tttgtcatat ttttaagttc cgagtctgcc atagattcaa atttaggttc taatgaatct
    18961 attatatcaa cagttttatt gaactttttt aattcttttt tatctgccat gttgaataaa
    19021 ttatccataa agctcatatt ttacatctct cctcgttctt tattgtacta ttattcattt
    19081 taccataaaa actatacttt taatattaat ttaaaaagct atcgataatt tatttatcga
    19141 tagctaatat gattaaatct aaattgcttt attcatgttc tattatacca tatccaccat
    19201 tattacgctt ataaactact gctatttcat ctgaatctat gttcttgaac atataaaaat
    19261 catgtcctat taattccatc tgtaatacag cttcttcttc actcattggc ttaacactaa
    19321 atttcttacg tctttgaatt actaattctt gattttcatc cccataattc tcttgttcat
    19381 cagctagatt tatctctgca tcttggaatc ttatactctt gttgtcttta tgtttatctt
    19441 gtaatctctt tttatatttt tttaattgtt tacttaattt atcatataca acatctatag
    19501 ctgtgtataa attttcctgc atatcttctg ctctaattat aggaccattt acagtagcta
    19561 ttgttacttc tattttttgt cttgcttttc tcgcacttac tgtaactttt acatcagtgt
    19621 ctggatgaat gtaattatct aacttactta gtttagtttc gattttattt tttattgcat
    19681 cagttaattc gatttgtttt ccagatataa ttatgttcat aaatacatct cccttcaatt
    19741 atgccagtat aagaaaattt atactttaat aatatattct acacgaactc tcataatcct
    19801 ctttttatta tttactttta tgattttttc tattcaattg atagaattat ttatcatttt
    19861 tagttacatt gatacttatg tggattaata ttattacaaa cacctgtgga taatgtggat
    19921 aaaactgtta ataactcttg tttttgttta tttaaagcta tttttactcc tatttctatc
    19981 cacaaaaatc tccattaatt gtttattaat atgtgaattc atgtcataag atggtaattt
    20041 taggaaatta tgtgtattat atgccaatat acctttataa ttttttaata aatataaacc
    20101 ctctaaaacc tccataatct atcttttctt tcattattta tatttaaaac taattaaaag
    20161 ttataaagca tactcaacaa taatttcata tttaatacct attaacattc atatacccaa
    20221 attataatta acttactctc tttatttaaa ctaagtattt tatattcata tactatcttg
    20281 aaattatatt tacgagttgt ttaagctttt gtctatctat atcttcaaaa ggactgttat
    20341 ttattctaat cgctgtacca aaatgaaaat tacttgcctt agttaattcc tttatctttt
    20401 ctatattatt aaaattaaga cctccaccta aaagaatttt tatgtgatta gaactaagca
    20461 tcatattctt tatcatttgt atgttatcaa ctatacttcc tttccctcct gatgttaata
    20521 tattcgtaat tttatcataa tccttcaaaa ttttaacact atctattgta tttgattcat
    20581 ctattgctct atgaaatgta acatcaagat tatcacaaca tttcaataaa acatttagat
    20641 ttttctcatc tatactattg tttttatcca atacaccaaa tacaactcca ttagcaccaa
    20701 tctctttaac cacacttata tctttttgca ttatacttat atcttcttta ctgtatacaa
    20761 aacttttcgc atgatgtctt atcattacgt tcacaggtat ctttacactg ttaacaacac
    20821 tttctataag accaaaactt ggtgtcaatc caccttcagt taaagcacta actaactcta
    20881 ttctatctgc accacaatct tctataattt tagcgtcttc tactgacatt cctattattt
    20941 ctaacataac tactcctttg ctaattcata tattgcattt gcaaatatct ttgcatttaa
    21001 tattatatct tctatatcca tgtattcatc aggattatga gctattcctt tttgtcctgg
    21061 aaatgaaggt ccaaacggaa ctatatttgg cattactttt gcatacgtac ctccattagt
    21121 tgttacggga gttccatcta atgatgtaac cctttcataa gctagctgaa gctttttaac
    21181 aagattactc tctttatcaa aatatacagg attaaaatta gaaattacct ctatatcgat
    21241 actatttgga aaactagctt ttatttcatc caatacatct ttcaatttat aactaaaatc
    21301 gtattttacc tttatatcta atatagtatc actattttct atattcagct ctttatatgt
    21361 aacaagcaca ttttcagaca tattttctat aaaacctagg tatacatctt cttcataatt
    21421 gattttactt tttattctaa tctttgccat gcctttttca ccatatacaa caggatattt
    21481 acaatctggt gtaaatccca taataggtgc tttttctttt tctaaataat atggtatatc
    21541 ttcaaatcca ctttcttcat ttgtaccaaa tattattcta actctcttat ctaattttaa
    21601 gtttaattct ttaatagcat ataatccata taaggcagac attataggtc ctttattgtc
    21661 tagtactcct cgtccataaa ttcttccatt agtttcttct cccttataag gctgatgctt
    21721 ccaaccatct ccttcatgta caacatctac atgtccaatt acacaaacat aatcatcact
    21781 atctccatgc tctgcataac caatataatt atccaagttt ctaaccttaa atcccaattt
    21841 ttctgatatt tctaatgctt tattaagtgc ctcccctact ttttcgccaa aagggaagcc
    21901 attttctgac tcccctttga cactaggtat ttttacaata tctataatat cacttaataa
    21961 atcttctttt aactcatcta ttttattact tatgatattc ataaaaatct ccttattata
    22021 aaactctatt ttaattatgt aaccactact ttactcactt acttctattg gtgcttttat
    22081 tctagcctca tatgcttcca tccactcatt tgatataacc ttatgtatag tctttccatc
    22141 tactctattt gaaatatata cagtaggaga ttcttcttta gttactactg aaaaagtaaa
    22201 atcagctata tttgtagcta ctccccattc acctttatta ttcatggcaa ctaatgataa
    22261 atcaccagct tttcctcttc ttttgataag ctgtttatca agttcattta ctgcaatatc
    22321 acatgcttct tgtggatgct tcccttcttt cataagtcta actatctcat aagatataca
    22381 tcccttcatt aaatcttctc ccaaaccagt agctgtagct cctcctatac tactatcaac
    22441 atagaatcct gaaccagata gtgcagaatc tcctattcta cctcttttct tcataaacaa
    22501 tccacttgtt gatgtagctg cacacatttt tccagttttg tctaaagata tcataccaac
    22561 agtatcatga ccagcatatg ggcttaatcc tttgtctaaa gtctcttttt ttctcttttt
    22621 ataatgtaac ttcgctctat cagtcaacat attttttctt tcaaatccat ttttatgagc
    22681 ataatcttca gcacctattc caactaaaaa attatttacc ttctcataac ttaacttcct
    22741 agctatgctg acaggattag aataatctct tataccagca actgcgccaa tatctaatgt
    22801 gttaccatcc ataaatgcag cgtcaagttc tacctcacaa ttttcatttg gaagtccacc
    22861 atatccaacc gatttataga atggatagtc ttcaactctt cttacagcaa gctctacagc
    22921 atcacctgct tccatgcctt tttctaaatg ttctgatgcc tcagttattc cttctagggc
    22981 cattctccat gtcgctatca ttccccacat gttaaaattc ccctccttaa attatataga
    23041 ttttactaat gtatgcttat tataaatagt ataatattta aacttgctaa taattaatac
    23101 tcttattgat aacaaaactc ttctttcatc aaagttatat cttctttatt tgctattgct
    23161 tttgccaatt tacatatgtt atttaatgaa tcattaagac caattccacc tttttttgga
    23221 gtcttaacta tacatatgtc atttttatat tcgtttatca catctatatt ttcttctgac
    23281 atagctgcaa aagctggtat atctgtttta tcatttatat ttttagacag tattgctgac
    23341 atcttagaaa agttttcgta attaaagctt ggtccacaga ttactacatc agggtttaat
    23401 ttttttacca tagctatcat cttactcgtt acttcttctt gattatttac aaaaaattca
    23461 tccccacaaa ataaggtagc tattacctta gattcattta aaaattgctc catcattaca
    23521 cctggtccta atgggcttga ttttccagca ggaggtatat tacttttctc ttttcctcca
    23581 gctccagctt gtatttggtc caatatcata actattttct tcataatctt cctcctataa
    23641 tactaaatca tcaaaagata tatcatcatc ttcctcttca acagtttcat tttcttctct
    23701 caagtattgc ttatctagca tttttataaa tggtatatac ataaatattc ctagtataag
    23761 aagtactaat tgtaagactg caccttgcca tcctgttgtt aaaaaaccag agaatataat
    23821 tggagttgtc cacggtaact gaactccatt tgtaagaggc actaatcctg ctgacataca
    23881 gaagtaagct attattatat ttattgttgg aactattgca aatggtatca atagtattgg
    23941 gttaagcatt attggtagac caaatatcaa tggctcattt ataccaaata ttcctggtaa
    24001 aattgatagt tttcccattg actttatacg tttactttta catattaata acatagctat
    24061 gactaatgat agtgtactac ctgctccacc aaatgtcgca aacatatctt ggaattgacc
    24121 tgttattatg tttggtattt ttgaaccagc ttgaaaagca gctaagtttt cagctgatag
    24181 aatttttaat attggattat acacagcccc aacaacacta cttccattta ttccaaagaa
    24241 ccagaataaa tgtaagaata tataagcgat tgccatagca cctaaactat ttcctaattt
    24301 taataatggc atttgtaaaa acttaaatat aaattcatgt atacttccat atggtgtagc
    24361 ttcaaaagct atcttaacta aaaagaaaac taccataact attgcacttg gtattaatgc
    24421 tgcaaatgat tttgatacag ttggaggaac tccatctggc attttcaatg tccatccttt
    24481 ttttacaact gcagcaaata attttacaga ggttattgct gttatcatac ctacaaacat
    24541 tcccttagaa cccatccaac ctagtggtat acctgttacc atgtaaactg attctgttcc
    24601 ttctggtgta tatggtatta caaatggtgt tattataaaa aaggcaacta ttgcaactgc
    24661 tgctgatgct attgggtcta catctatttg ctttgcataa gaatatccaa tccctaaaac
    24721 tgcaagtaga gtcattactt caaatgttgc tacagttggt tgttgcaatt ttacagccca
    24781 atctggacca aaaatttgac ctacaaattc attccatcct tttataggaa aatttgctac
    24841 taataaaaat aatgaaccta ttattaataa tggtgatgat actaaaaaac catctctaat
    24901 tgcaattaat aatttatttt tacctatttt ttcagccaat ggcataagta ttctttccaa
    24961 tctattcatc tatccaccct catttcctat tattttttac tataattcta cgcttgttta
    25021 gattttatta gctttatagc aagttttaat actttttccc cattcatagt tccataatca
    25081 acttcattga ttatatctat aagttttcct tgagcttcaa actttggttt taactccttt
    25141 agcatgtatt taacttgtgg tcctaataat atacaatctg gatttttttc ttcaactatt
    25201 ttgtcaattt ctgctatcgg aaatgcctct acttctattg gtaaattgtg ttcatctgct
    25261 accttctgca ttttatctgc aagtaaactt gttgacatac caaagctaca aaataaatat
    25321 acttttcttt tcatttttta aatccccctt aattttttta ttttaatttt tgaatttctt
    25381 ttcttaacat aatcatttct tttataaggt ttctctcact catacaagtc attaaatggt
    25441 cttgagagtg aatcaataaa atagatatat ctgatttatc tcccccagct tccttttgta
    25501 taagttcagt ttgtacttta tgtgctttta aaatttcttc atttgcattt tcaattaatt
    25561 catctgcttt tttaaaatcg ttttcctttg catagtcaag tgcttcataa agcatagatt
    25621 ttgcatttcc agcatgaatt ataatcttta aaactatttc ttccattttt tcctcctaag
    25681 ttgcttctct acttttagat tatttatacc ttacaaacat ataatatctt ataaaaacaa
    25741 aaaaacatgc acaacttttt ccatcacatg gaaaattttg tgcttatcca tagttcacta
    25801 tgaattttat ataagaatat cttttacctt ttcaatgtca catttactta gaatatctag
    25861 catattttca tcctctgtta aattagatat ttctttaaat aattctctaa atacttttct
    25921 atcatcatct tttttatacg ctaacaaaag tataatcttt atatcctttc catttctaat
    25981 ttttattggt gtttttaacc ttgcaaaaca taaaacagat tgttttatat atttcatgtc
    26041 tccatgaggg aataatattc cttttccaac attggttgga tatgactttt ctcttgttat
    26101 aatagattgt aaatactctt tttctgcata atgtttttca attaataaat tagttaaata
    26161 tgttatagct tcataaatat catcatgttc taactctctt attaaataat cagacatcat
    26221 taatctgttc ataagtttat atgaagtgtc tttttctttt attttgtttg tgaactcttt
    26281 aagtttattc tcatcctcat cagtaaataa tggtgttgtt ataaaaattg gtttttggat
    26341 attttcatac tcttgaaatg caattataaa atctatagtc ttattaaagt tatctctttt
    26401 taaatcttcc tctcttaatg tttcaataat ttttatgtta tcaaatctct tttctatctt
    26461 tacttttaat agcatactta ctccaaaact aaatggacat acaatacttg cctttttatt
    26521 tgattccctt ttgctgtatt ttctttctag agaggtttga aagtgaagtg taagataacc
    26581 aatttcatct tccgttattt gttttccttt taattcaatt tctattgaac ttgatattac
    26641 tgtaaataaa aaacaaaatt tagtttttat gttatctaaa agtggatttt ctaagtaaac
    26701 attgcttttc atttgatgca ttgtaacatt caaatgacat attaattgtt cataaagtaa
    26761 tatatcatta ttaaaattta ttcctgattc ttctgatact aaatttataa ttttttttgt
    26821 ataacttgct aattcttcat ttagatataa agatttctta atattattac tatttatttg
    26881 tttgttcata cccaatatta aagattctat aaacaatttt tcttcataat ttaatactat
    26941 ggataaatta gatttcaatt catcttctat atccaatata agcattttta gcttaagctc
    27001 tttcttagta tttgatactt ttaaagaatt tcccagcctt attcgtatta tatttataag
    27061 caaaaatagc atcaattgct tgaatgatat atctgtaagt cttatatcca tatcctcttc
    27121 tatagtaagc attatgtttt ttacaattat tatatctact atatggaagt actctatttc
    27181 atctatgttt aatctgtatt tatctatata tttaaataag aatgatacta acattattct
    27241 tatgtctttc tcatcccctt caatatatat accactgcct tttttagtcg agatatttaa
    27301 attataatta tttaatgcta ctgataattt acttatttct tctctgacaa tatttttatt
    27361 tatgtacaac ttattagata aatcatttaa agtaactttt ttattattac aaagtagtaa
    27421 atttagtatc tcaacttgct tatgaagctc actatttact cttttaactt cacctcgcaa
    27481 cttctgattt accagtattt tgtctgttcc atctccacta aatttaatcc caatattagg
    27541 ttttcttttg atataagcat tacaaaaagt agataaccat tcatcaatat atttgcaatc
    27601 atttcttatt gtcctttctg aacactcaaa atcattggca atcttattaa catttataaa
    27661 ctcttctttt aatagcaatg ttgttaataa attttcttgt cttttattca aacatatcac
    27721 ctctttatga ctcaacctaa atttattata tataaaaaag agctatacat aaaggtatgc
    27781 tagctctctt ttaatatgat atttagctga cctggttttt gaccccacac tcgcctactg
    27841 gagggttcgc ttgggttcgc cgcactacta ctctctctcg attttattat cggtaggtct
    27901 tacttctaag ccgcaccatg atcattaacc caatttttag tgttaactta cgtggatccc
    27961 tttgcctgcg actggcttgg actttcaacc acagacctaa atatcactta ttcattttaa
    28021 tattttaaac taaaattgtc aatatataag attattccat aatataatta tcatttgctt
    28081 ttgtaagtag agtcaataca aaaaccttat tagcacctga taactttaaa agtttagaaa
    28141 tttcgtttgt tgttagacca gtagtaaata tatcatctat taaaagtata tttttattat
    28201 ttattaattt aacattttct ttaacaacaa atacattttt taattcttct tttcgttctt
    28261 tcttattcaa tttatatagc attcttgtat atttttttct acttatgcaa tctaataatg
    28321 gaatatttac tattttactt aaattaaatg ctattttttg tgcctgatta aaacctcttt
    28381 tatttagcct ttttttatga agtggaacaa ataatatata atcaaatttt atgttttcta
    28441 aatataattt ttctttcatt atttgagcta tatacttagc cataaaggtt ttttggttat
    28501 acttcagccc taacaccatc ttttttgtca catcattgta ttcaatacaa gatattgatt
    28561 tatcaaaata aaagtcttta ttaaaacaat aactacactc ttcaataaat tctttttcaa
    28621 ttgaatgacg tattattggt tttccacact tcatgcaacc atcttgtata aaattcattt
    28681 ctttaaaaca acttttacat attgaataag tatttgtttt ctttatggat ttatcacaaa
    28741 ttatgcaact tatattctca ggatatataa aatccaaaca tttattaatc atatttttaa
    28801 aattcttaat aaaattatca ttgcatattt tcatatttat tctatcaaca acccttcttg
    28861 tttaaattta tttagcttat atcctaaatt tgaatacctt tgattaactc tattattttt
    28921 tatcatatat tcaagatatt tcacatctcc aactaataca actaattttt ttgctcttgt
    28981 tactgctgta tataataaat tacggctaag taacatagga ggagcccacg ctattggcaa
    29041 tactacaact ggaaattcac ttccctggct tttatgtatt gttgtacaaa aactatgatc
    29101 aatttcatct aattcatcat ataggtatga tacaatttta gtctggtcaa ataatatata
    29161 aatagttttc ttatctttat ctatgtgata aacatatcct atatctccat tatagatacc
    29221 ttctccactt tctttttggt cttctgtttc ccattttttt gtataattat tttttacttg
    29281 cattacctta tcgccaactc taaataatct tttagaaaaa ttttcttcaa ctttaaattt
    29341 ttcttttttg ttaagatact tctgtaactc tatatttaga ttagtaactc ccaattcacc
    29401 ttttctcatt gaagataaaa cttgtatatc ttttaattta tcaactttat aaaatttagg
    29461 taatctttca ttaaccaatc ctattatttc atttaaaatt tcttcattag ttgattttct
    29521 tataaaaaag aaatctttac cttttgtatt tagataaagt ggttctccat tgtttatctt
    29581 atgggcattt acaattatca tactttcttg cgcttgtcta aatatttcat ttaatttaac
    29641 tacatttatg atattagaat taatcatatc cttaagtaca tttccagcac ctacagaagg
    29701 taattggtca ctatctccaa ctaaaattac tcttgttcct aactttatag ctctaagtaa
    29761 attgtacatt aagataatgt ctaccattga tacctcatca acaattatta catctgaatt
    29821 tattgggtct tcttcatcct taaaaaaagt taaatcatca tctgttgaaa atcccatttc
    29881 tagcaaccta tgtatagttt ttgcttcttt attagaagtt tcactcattc tctttgctgc
    29941 tcttcccgtt ggagctgcca gagtcacact tttaccatta ttttcaaata tatctatgat
    30001 agcattaatg gtagttgttt taccagttcc tggtccacca gttataatta atactccact
    30061 attaacagat tctttgactg ctaaaatttg tttttctgct aacttaattt tcttatcttc
    30121 ttctaaaaca ttaatctcgt tatctatatc tatttttaag tcttcaaatt catactgtga
    30181 taattttact atttgactac atactccatt ttcagctaaa tagtatggca taaggtatat
    30241 aagattttca ttgtttactt tctctatatg tattttttga ttataaacta gcatcattac
    30301 acactctttt attatttctc catttagctc caataatttt tccgaatctt gtattagaac
    30361 atgctctggt aaatatgtat gcccattact taaagattta tttaaagtgt ataagattcc
    30421 ctgacaaatt ctatctttag agtttttatc tataccaatt ttacttgcta tactatcagc
    30481 tactttaaat ccaattcctc ttatatcttc agcaagttgg tatgggtttt tatttataac
    30541 ttctattgca ctgcttttat attttttata tatttttagg cagtaatttg gtgttattcc
    30601 aaaaggagat agctgtatta ttatatttct caattctctg tcttcttcat aactttttac
    30661 tatttgtttt acttttttac tccctatacc ttctacttct tgtagttttt ctggtgaatt
    30721 ttgtatcacc tctaacgtat ctacaccaaa cttatcaatt attctttttg ccattttttc
    30781 accaatacca tgtatcattc cagaagataa gtaaacataa attccttcta aggatgatgg
    30841 tgttactggc ataaaactat tcacttcaaa ttgagtccca taaatcttat gatttaccca
    30901 ctttccttca acttctatgc tttctccaat tgctaaagta ggcatacatc ctacaaccac
    30961 aatttcatca ttttcattta ccaggtgagc tattgtataa ccattatctt cgtttttaaa
    31021 gactatttca cttatcattc cttctaattt ttccattttt atctcctaaa aatactaata
    31081 atcgctccat ttaaatgtta gtataatttt atatattaaa actatgataa tcgtagaaaa
    31141 gttttatatt tttatactta acgtaatttc caactattat acattttatc agaaacaaaa
    31201 aaaatctgca tccataaaat atggtaccat gaaatacagg taccatattt aaatagctat
    31261 tttattttaa ttactctata tttagagtat aaattaaatt tcataagtat attacagtac
    31321 taacttattt cagatttatt ctccttgtac ccctcagttt tagtaattct tctataaagc
    31381 atacatagta aaattaaaat tcccatgtaa cctgtatatg gatatagtgt acctactaac
    31441 ttatcaaatg gcagtaaacc tcctatacaa gctaatatac ttaccactat tgttataatt
    31501 ttaaacttag ggtggtcatc ttctacaaat cggtttgtta ctgaccataa caatggtaca
    31561 gctgtagtgt atattcctag taaaagtact actgagaata aaatacctat tattggagat
    31621 atcttatcag ctaagtaaag tgctggtatc tcttttgtgt atatatttcc tatgtctgaa
    31681 agtaatgcca aattcatcat tatagctgct gccataagtg ctactccacc tactatacca
    31741 ccccatacag catctttctt atttactgca gaagctccca ttcctgtcaa gaatgttatt
    31801 actattatta cattgtaaca agtataaagt actcccgaga aatatccatt tgatgtagca
    31861 gttttaatat ttaaactatc aaccatattt ccagcatttg ataaagcatc aatattgcta
    31921 aataaactaa ttgctcctac taaaagtgta aatattatta ttataggtcc tatattacct
    31981 agtattttag aaagccttgt caaacctaat gaaacagtta ttagggaaac gattgccata
    32041 ccaatcctac ctacatatgg atttaatcca tagtactctg ataatgtagc tcctgctcct
    32101 gcaatcatta ttactaatgt tccaaataaa aacaatggac caaaccattc aaaaaatgtt
    32161 ccaagatatt ttccacaata tacttgataa atttttattg gctccttaag tttcaattct
    32221 cttcccttat ctataacttc tgctccaaac catgaaaata gtaccataga gattattcca
    32281 cctataatac ccgcatatcc ataaaacgta aaaaattgca gtatttcttg accagttgca
    32341 aatccagaac ctattacagt agcaacataa gctccagcaa aacttataac cgttttaata
    32401 tttactttgc ctgacataca ctactccccc caattaaaat tatttttaaa gtgtaagttt
    32461 attaagatta tattagactc attattgtga tatttcaata tatttttaat tatttaacat
    32521 tagttaacac attcttaacc atcctttaac atatatccca taatatgtat aaatttagta
    32581 gagtattata taaaatgtat tgaccagatt aaatactgtt tattcattat atgatgataa
    32641 attaacattt attaaaaata tttaactcat aataaaaaag ctgttagaaa gtaatcgtaa
    32701 aattacattc taacagcttt ttagttaaaa attattcacc taaagcatct tttcttaact
    32761 gctcaacctt gtcagttctt tcccaaggta aatctatatc agttctacca aagtgaccat
    32821 aagcagcaac ctttttataa agaggctttc ttaaatctaa gtctcttatt atcgctcccg
    32881 gtcttaaatc aaagtgttta tttactaatt caacaagttt ttcttctgaa accttgcctg
    32941 tcccaaaagt atctataaat atagataatg gtctagctat acctatagca tatgcaagtt
    33001 ctatctcaca tttgtctgca agaccagccg caacaatatt ttttgcaaca tatctagctg
    33061 cgtaagcagc agatctatca acttttgtag ggtcttttcc tgagaaagct cctccaccat
    33121 gtctagaata tccaccataa gtatctatta ttatttttct tcctgtaaga cctgtatctc
    33181 cttgtggtcc tcctataacg aatcttcctg ttggatttat ataaatttta gtctcttcat
    33241 caagtaattc tgctggtata acttccttaa taacatgatt aattaaatct tctcttattt
    33301 tatcatttga tactgtttca caatgttgag ctgatataag aactgtatgt actcttacag
    33361 ctttacttcc ttcatattca acagtaactt gagtttttcc atctggtctt aaataatcaa
    33421 ctaaacctgt ttttctaact tctgtaagtc ttcttgataa tttatgtgcc aaagatatcg
    33481 gaagtggcat taattctgga gtctcattac atgcaaatcc aaacattata ccttggtctc
    33541 cagctccaac tttttctatt tcttcttcta tctcttctcc agtcttactt tctagaccct
    33601 catcaactcc catagctata tctccagatt gttcatcaat agaagttata actgcgcaag
    33661 tatcacaatc aaatccatat tttgctcttg tatatccaat ttctcttact gtttctctta
    33721 ctaatttagg aatgtctaca taagcagatg tacttatttc tcctgctact aaaactaaac
    33781 cagtagtaac tgtagtttca caagctactc tagattgtgg gtctttttct aataatgcat
    33841 ctaatattga atctgatatt tggtcacata tcttatcagg atgtccttct gtaactgatt
    33901 ctgacgtaaa taaatgtctt gccattttta ttccccctta tttattctta atttttatta
    33961 taggttttac cttgttttta attttagaaa tttactcaaa ataaaaaaac ctctccttta
    34021 taagaagagg ttaataatcg taattacata ctaattaact gacctcatct ttcaagactt
    34081 ttgtcttgta ggaattagca ccttttccca tttgggtggt tgctgggctt cacagggcct
    34141 atccctcagc ctctcttgat aaggtaatca agatttaagt tttgttttac tttactagct
    34201 tactattttt ttaaaatttt gtcaatattt ttagtcatat tttttctatt cctttttttg
    34261 taagacaaat aaaattttct atctatctga aatttaaagt taggtctttt taatgctttt
    34321 tattcatata tttttttgaa gggggtgttc ctattgtatt tagctaaagt aaactacagc
    34381 gatgatgatg ttatacagat gctaagttca gttctaaaaa ctataattaa tgaaaatacg
    34441 attgttgttt gcataggaac agacagggct ataggtgata ctttaggccc tttagttgga
    34501 acaatactta agaacagtaa ctttaaatac cccgtttacg gtacactaga taaccctatt
    34561 cacgccttaa atatatatga gtctttagat actataaaaa atacacatat acaaggtaac
    34621 ttcttggcta tagatgcttg cttggggtcg cagagtaaca taggtaatat acagattaga
    34681 gaaggaccta tcctgcccgg aaaaggtgtt ggtaaaaaac tacctcaaat aggaaattat
    34741 tctatagtag gaattgtaga taaaattgat gaaaataata aactttcatt taataatata
    34801 cgccttagct ttatattaga tttagctgaa acaatagcct tggctttatt agtatccacc
    34861 taatctaaat ataaactaaa tgataaaatt ctttatataa aaaggtagat attttagagt
    34921 atctgattaa atactccaaa ttatctacct ttttactttt atatttcaag tttttatttt
    34981 tctactaaag caaacattaa ttcaccttta cagacaagtt cttcacctac atatgcctta
    35041 gcatctgcct tagctatatt ttttctaaga gttgtcattt caacttccat agttaatgta
    35101 tctcctggtc ttacttctct cttgattcta actttatcta tacctgcaaa tacacctagt
    35161 tttcctttgt tttcctctac agacatcatt gccactccac atacttgtgc tagagcttct
    35221 actattagta ctcctggcat caaaggatat tctggaaagt gaccttggaa aaatggttca
    35281 ttaactgtta tattctttat accaacagct cttttcccaa cttctagctc tgtaatcttg
    35341 tctactaata aaaatggata tctatgtggt attaattttt ttatttcatc tatatttaac
    35401 attctaaact ctcctaatta ttttcttttg aaagacccac cagtttcaag taagtctatt
    35461 gaacctaaag ctattcctgt accttttgca acacaagata atggttcatc tgctattgta
    35521 acagttattc ctgtcctctg ttctattatt ttatctaatc catataataa tgaacctcct
    35581 ccagtcatta ttataccgac atctcctata tctgctgcta actcaggagg cgttttttcc
    35641 aaaacagcat gagtagcaac tactatttgc tcaacacatt ctctaagtgc ttccaacatt
    35701 tctgttgaat ttatagttat agaactagga agtcctgtaa ttaaatttct tcctgttatc
    35761 ttcatatatt tttcttcttc tctcttaaat gcagaaccta tttcaaactt gattttctct
    35821 gctgttcttt ctcctataag aagattgtgt tgttttttca tatatttaac tatagcatca
    35881 tcaaatctat ctccaccaac ctttatagat tcacttacaa cagctcctcc tagagatatt
    35941 acagctatat ctacagtacc tccaccaata tctataatca tattaccatt aggctgtgat
    36001 atgtctacac cagctcctat ggcagctgct attggttctt ctatgatata tacttctcta
    36061 gctccagctt gtctcgtagc atcttctact gctctttttt ccacttctgt gacacctgtt
    36121 ggtacgcata ccataattct tggcataaaa aatcttccaa atcccttttt atcaactatc
    36181 ttatctataa aagattttaa catcttctca gttacatcat aatctgaaat tacaccatct
    36241 cttaaaggtc ttatagcgac tatattacct ggtgttctcc ctatcatctt tttagcttct
    36301 tctcccacag ctaaaacaga atctgttctt ttgtctatcg ccacaacaga tggttcttct
    36361 aaaacgattc ccttaccact aacataaaca agcacatttg cagtacctaa atctatgcct
    36421 atatcagctc cagccatttt ttcaactcct ttatttataa tattagatta tattataagc
    36481 ctattttaat actcctataa taaagcatat acaaaaatct cgctttaatc aagagaaaat
    36541 aaatcttttg agagtattat tattttgcta tttataatta cttaatatat ttctttttat
    36601 aatttaattc aataaaaaaa gtaggttttt aacctacatt tttttatgtt ccttctcata
    36661 ttttaatttc gttgctaatc cccctctaat atgtctctct gatttatttt tgtccagcac
    36721 atttttaact tctttggcaa gggatggatt tatttccttc aatctttctg taacatcctt
    36781 atgaattgta cttttactta ctccaaatgt cttggcggtt tgtcttactg tagtgttttt
    36841 ttcaagtata tattttgcta ccactatggc cctttcctct atatgagatc tcaaagatta
    36901 cccccctcac ataatagctt taatgatata tatgtaaaaa caccaaaaaa tataacaagc
    36961 ttaaccaaat atgattaaac ttgttatact attacttaat tagactcatt gggtctatag
    37021 atttttctcc actatacgcc tctaaatgca catgtatacc ttcttcactt tctatttgag
    37081 aagtactacc tacagtacct agagattgtc cttctgttac cttctgttct ttcttaacac
    37141 taacattttt atctagattt gaatatacta ctacaatgtt attgtcactt tttattttga
    37201 ctgattgtcc atattcttca tcatcaaata catctactac tattccgttt aatagagatt
    37261 ttacttcttt gcctttagta caactaatat ctacaccttt atgagtttcc caaacatcaa
    37321 gtgttttaga ataactaggc tctttttctg aatatcctct tattacttta tctccaatgt
    37381 aatttaattt tgttgtactc gatttatttt cctcttttga cttagctttt tctaagtttt
    37441 gttttgaatc cgttgcagta gggatagcat ctttcttgtc tttttcaatt aaatgtattt
    37501 cctcttcact atctttatta gcattttcca taattccttt attagaagcc aatttatcta
    37561 cattattatt tgtaaaccaa actccaccta ctgctaataa acaaacacat acaaacaaag
    37621 ataaataaaa accatctttt tctaacagct ttttcttcat tttgcacctc caaatatatt
    37681 tctacatttg gattattgac gtgtttttgg cattttatac aattattagt atatatcttt
    37741 tatttttgta tctgtgtagt aatgtgataa tatatcataa tatttataac cctcttctgc
    37801 cattccttct gctccccact ggctcatacc aacaccatga ccatatcctt ttaccacgaa
    37861 atctatgtat ccttctccaa attttatgtc aaagtttgca gagttcaact taaaaacagt
    37921 tctaatgtct tttccagtca attctttatt tcctaatttt attttttcta cagttccggc
    37981 atcacttctt ttagttattg aaacttgttt acttaaatta ttcacatcta tcacaatagt
    38041 gctatatgcc cttctaagac ttttaacaaa atctgtgtta cttattttaa gtgttgacgc
    38101 aaatttaggg gaatacttat cataaggact ttccacagac tttaaataag gatattttgc
    38161 agaaaatact tcttcactat tttctgtttt tcctgaagat gtagaaaaat aaagaggaag
    38221 tatcgcctta tcattataag ttattatttg acctttagtt cctctaactg cttcttggat
    38281 tttggaatat ttattcttaa tccattcttc accatttaat tttttaagag tatcataact
    38341 cttatattct tgacaatgtt tataatcagt acatactact gcatttttat gtttactaga
    38401 tttaccatgt tcctgtttat atactacata tgttctagct gctacagatt gagcttttaa
    38461 agcttctata tcaaactctg aagacatctc accagctagt actccacata aatagttctc
    38521 catgtccatc ttttcggttt tccctgttat atgattatat acattgataa ttggtgcttt
    38581 tttatttaca gtttcatagt tcactgtatt tttttcttct ttattacctt tgtcttcaat
    38641 atcacttttt ttaatagttt tgtttataga tactgggctc tctggttttt cagttaattc
    38701 tacattttta taagaaacta aagttataag tgatggaact aacacagagc aagtcacaaa
    38761 tcctaacaaa acaaccaatg ggttcttcat atactctcct ccttagcttt taaaaccttg
    38821 tatttaagta tatgaacaac cctattaatt tataactttc ttctataaac caattattaa
    38881 tcttctacta tttcgatttg accacctagt gctgttatct tcttatctat atcaacataa
    38941 cctctttgga tatggtatat ttctcctatt tgagtttctc cttctgctat aagaccgcat
    39001 aaaataaggg ctgcccctgc tcttaaatca gtagcgttta ctgctgcacc gtgtagctca
    39061 tctacaccat taacaaccgc acttcttcca tctattttta tattagcacc cattctatta
    39121 aattcagcaa catgcataaa tctgttttca aaaacagttt ctataactac accagttcca
    39181 ttggctacag taagcatagc cataaattgt gcttgaacat ctgtaggaaa tcctggatgt
    39241 ggtaaagttt ttatatctat tggttttaat acttttggac ctacaactct tacagaattg
    39301 tccatttctg ttatttcaca accagcttct cttaatttag ctacaactgg ttttaaatgc
    39361 tccattaaaa cattttcaac agttatatca ccttttgtca tagctgctgc aaccatataa
    39421 gttgctgctt ctattctgtc tggaattaca ttatgttctg ctccttttaa ctctttaaca
    39481 ccttttattt ttatagtatt agtacctgca cctttaacat ctgctcccat ttcgttcaag
    39541 aagtttgcta aatctactat ttctggctct tctgctgcat tctctattat tgtagtccct
    39601 tctgctaaag aagctgccat cattatgttt tcagttgctc caacagatgg gaaatctaaa
    39661 tataatttat ttcctactaa cttttctgtt gctgcttcaa caaaaccatg gtccatttct
    39721 atctttgcac ctaaagcttt aaatcctttt aaatgtaaat ctataggtct tgttcctata
    39781 gcacatcctc ctggcataga aattttcgta ctattaaatc tagcaagcaa tggtcccata
    39841 accaaaaaag aagctctcat ctttcttaca agctcatatg gagcttcaca agtttttata
    39901 ttgcttgcat caacagtaag tgtattttct ttatattcaa cttctgctcc tacatgtctt
    39961 aataaatctg atataacatg aacatctctt aaatttggta ctccatttaa tgttgatttt
    40021 ccgtttgcta ataaagtcgc tgctattatt ggtaaaactg cattttttgc accatctatt
    40081 ttaacactac ctttaagtgg attactcttt tttactatta tcttagccat attttttgat
    40141 ctcctcataa ttaataatct aattttataa ttggtgtggc aatatatatg agtgtttttc
    40201 cgtcatcctc attatattta attcctatat ttaaattaat tttttcccct aaatattcta
    40261 attcataatc atttaatact ttactatatg ctgttataga taaaaagttt acgtctttaa
    40321 cttcatctat ttgcttagca ctcatattat ataatatttt ttgtaaaata tcatcatatt
    40381 tgttgttttg taaccttttt gtgtattctc caacaataca agcagacaca tctacttgag
    40441 atgaatattt atttaacaca ttttctacgt tactataaat aacctcttta tgtttatata
    40501 ctttgttatc caatatgtca actactatgt aactttcttt gttattttta tttattcctt
    40561 ttatagaaat ttctttatcg ctacctttta tagtagcaca taattgtatt ttatcgccag
    40621 accctttttg attccaattt attccttttt tatccaactt tagattgtta attatttcta
    40681 taaaaatatt atgaaattct ttatttgata aattatgatt tataacagaa ttaattttaa
    40741 cattataaaa tttaaaattt gcttctgata tttcaagagc tttacttaat ctattgtatc
    40801 tagtatttat acttgtatct gcataagata ttgtcattcc tataatcatt aatataaaaa
    40861 agcttatagc taattttact tttttcatag taaaaaatcc cccttaaata tgtttttctg
    40921 tataatagaa ttattaacaa atttaagaga gatatacttt ctcaatatgt atttttatac
    40981 atttttgtat ttttcatcta ataatctctt tcaaactctt caatctcaat tttctctatc
    41041 cttctcctgg acttgccaat ttcttatatg tatcaaatac atcttgcatt aattcttata
    41101 tattctcttc tgtaatataa attacttagt ctatacttaa atctataatc tataatctat
    41161 aatctataat ctataatcta ttcatatttt accaacaaaa taaacagata atactttttt
    41221 atcaatttcc atagctccaa atgaacatac ctaagtacac ttcccaatac acaatttaca
    41281 cactcttcct aataaatcaa aaacaccttt gttttaaaca actctattgc actttcaaaa
    41341 actttatttg tgttctccca cttcaattaa atttgaatta tcaagactcc agcccatcca
    41401 tccatcacta taaagggagg tattttccac tcctatgaca tttgcatatg ttaatacttc
    41461 agcagcacgc catccacttc cacacataaa tataagctgt ttattcacat ctatattttc
    41521 cttgtcccac atttctaaaa tctcatattt atttctcatt gtattatcaa tattacgata
    41581 ttcttctagt gaaacactat cactacctga atgtccatat aaagctcctg gtatacgccc
    41641 ttttttatca taatatgtat atccagatac ttttcctata tgttcatccc aagtacggtt
    41701 atctactagt ataaacttat ttttttcttt tagtttttgt cttaattcag atgtagtaac
    41761 aattaattgt gagttaactg gtatatctgc tccaaaatta gtacatgaat gttttggatt
    41821 actaataaat tctaattcat atcctgctga taaccatgag tttgttccac cattcaaaac
    41881 tcttacatct tttactccta tatatctcaa tataacagct agtctataag atgccatagg
    41941 tgtactactt gaaactatta cagtatcatc ctttgttaat ccatagtcta aagcaaactt
    42001 agttaagtta tcatcattat caagcatcca tgttggagga ggttctataa tatcagtatt
    42061 tacatgaaca cttgtaggta tatgcccttt tgtatacgat tcttctctcc aactcgcttc
    42121 aatcattttt atattctttg aatcttcaaa tgtctcagga atttttccat ctaatatatc
    42181 ttttattata aaagcaggaa ctatcatttg ataattttta tatctttcca ttggaagatt
    42241 ttcgtcgtct gcccattgtt taatatcata tttatataga tatttatatc cttttttaga
    42301 taaataatct gctacaacca aagcatcttt tccatttgca tcatacaata ctatattttt
    42361 atctaaatca attcttttag ttttcaatgc ttgttctaac acttcatctt ttctatctga
    42421 gtatacactt aaccaattag cagaaaaatc tactgctcct tttatatgtc cacctctttt
    42481 tactccatct aacttccagc cattataagc atcatttaag cgagtatcaa ctataaccca
    42541 agcgttatct tgcacttttt cttgtaaaac ttcagtagat attgccttaa gattaacaaa
    42601 ataatcttct ttcttttctt ttttaaaatc atttgtcata atttatactc ccaatttaaa
    42661 aaaattttta tttgaaatta ttttactaaa ataaagtaat tttcttatac tctattagct
    42721 tcattatatt cttttataaa tagttcctta tattcttatt ttaaactact cactttatct
    42781 tatcacaagg ttactaatac cttgtatttt aatttttctc ataagctatt atttttttat
    42841 ctgatattga ctatatgttt attaagtact cactatattt tatatgaact atatttatcg
    42901 ttccctatac ctggtatggt ctatatattt atcattttac agatgattta atatcaatct
    42961 ttttataaaa tttattatag caaaaacaaa acaaatttaa tatttatcta aaaaactcat
    43021 cctataaaat acaaataaca ggatgagttc ttttcaataa atattatcta tttaattttt
    43081 aatctgtttt tattcaactg ttactgattt agctagattt cttggcttat ctacatcgca
    43141 tcctcttagt acagagacat gataagcaag taattgcatt ggtacaactg ctaatattgg
    43201 tgataaaata tcatctgaat ttggtatata gattatttta tcagcagctt tctcaacatc
    43261 cttattatgg ctttgagcta ttgctactac ataagctcct cttgctctta cttcttccat
    43321 attagaaacc attttttcaa ataacttttc ttgagtagct atggcaatta caggagttcc
    43381 tttttctatt aaagctattg ttccatgctt tagctctcct gctgcaaaag cttctgcatg
    43441 tatatatgaa atctctttta atttcaatga accttccatt gctaagctat agtctatacc
    43501 tcttcctaaa tagaatgcat gttcagaact aacaactgtt ttagcaactt cttttatata
    43561 ttcttcattg tctagtattt cttgtatttt tgatggtatt tctttcatct tagaaatcat
    43621 actatcataa aattctctag ttattgttcc ttttttgata gcaaagtcta aagctatcat
    43681 gtataaagaa gttatttgag tagtatatgc cttagttgat gcaactgcta cttcaggtcc
    43741 tgcccaagta tagaatacat catctgattc tcttgctata gatgaaccta caacattagt
    43801 tattgaaagt attcttgctc ctttagcctt tgaatctctt aatactgcta gagtatctgc
    43861 tgtctcacca gattgactaa ctaatattac taatgaattt tcatctacaa atgggtcgct
    43921 atatctaaat tctgatgcta tatcagttat tactggtata tttacaaatt tttctatggc
    43981 atacttacca agtaatccag cattatatgc tgttccacaa gccactatat aaactttatt
    44041 tattttttct aagtcttctt tagaaatatt tatactgtct agaattatat ttccattatc
    44101 atcaagtctt ctttctaaag tttctcttac tcctgttggt tgctcatgaa tttcttttga
    44161 cataaagtag tcatatccac ctttagacgc agcttctaca tcccaagtta catgaaatac
    44221 ttctttttca acaagatttc tattagagtc ataaactgtt acattttcgt cttttagatg
    44281 aactacttct ccattttcta agaaataaac atctctagta tattttaata atgcaggtat
    44341 atctgatgct ataaagtttt caccttcacc aacaccaact acaagaggac tatcttttct
    44401 tactgcaact aattcatttc catgttcttt acatataaca cctaatgcat aagctcctct
    44461 taattttgat atagttttgt atacagcatc taataaatta ccctcatagt atttatctac
    44521 taaatgtgct attacctctg tatctgtttg agattcaaac ttaacacctt cagaaattaa
    44581 ttcttctttt atttccatat agttttctat tatcccatta tgaacaactg ctattgtttt
    44641 ggcttgatta aaatgaggat gtgagtttac atcagatggt tctccatgag ttgcccatct
    44701 cgtatgccct atacctaaat ttccatcaat aggattcttt tctaagtctt ctgcaagtac
    44761 agaaagtcta cctttaaact ttctaatatt aagttctttt tcattagaac tattaactgc
    44821 tactcctgct gagtcataac ctctgtactc tagcttagaa agcccttcta ctataacttc
    44881 tgctgccttt ctacttccta aatatccaac tattccacac attatttgtg tcctcctaaa
    44941 aatattattt atatatttta agagttaggt catataagtt tactttggct tccctttgtc
    45001 cataggattg ctcctattat ttaaagaagt ctttcgatgg taaacacacc gcagagcatc
    45061 cgccgacaat tcgattaact ctgacctcgt caacttagtt cctatattac tcaataatta
    45121 tgtctataac taagttctgg cgcttagaaa ggtgtcctca gtatttatat ctgagaatac
    45181 cctttaaatt acgataattt ttcttgtata agattagcta agtttgttgc taattctttt
    45241 atttgacctt cctctttacc ctctaacata actctaacta atggctcagt tcctgatggt
    45301 cttatcaaaa ctctaccatt tccgtctaaa atagattcta ttctttctat ctcagttttt
    45361 atttctggat attccatata tttgttctta ttttcatttt ttattgttgc atttactaat
    45421 acttgtgggt attggctcat tattgatgca atttctgata aaggtttctt ttcttgcaag
    45481 attatatttg caagtattag agaactaagc actccatctc cagtagtgtt gtaatctaaa
    45541 aatatcatat gtccagattg ctctccacca agattatatc catttttaac catatcttct
    45601 aaaacatatc tgtctccaac tgctgtagta gataaattta ttccattttc cttagcagct
    45661 attgtcaacc ctatattact cataactgta actaccaaag tatcttgtgc caatttatta
    45721 tttttcttta aatttaaggc actcaatatc attatatggt ctccatcaac aatttgaccc
    45781 ttttcattta ctgcaattaa tctatcagca tctccatcat atgcaagacc taagtcagca
    45841 ttatgttcca aaacagccct ttgaagttgt tctggatgtg tagagccaca tttatagttt
    45901 atattatttc catctggtga actatttatt gaaattacac ttgctcctag ttcatcaaac
    45961 actattggtg ccactttata tgacgcacca ttagcacagt ccaaaactac ttttaatccc
    46021 ttaaagtcag tgcttattat tgattttagg tagtctacat agtctctttg tgcacaatgt
    46081 tcatgtaatt ttcttcctac attttcacca attggaagac aatcaatttt gtctatatcg
    46141 tctatatatt cttctatttt caattcaatt tcatcatcta atttatatcc attcttatta
    46201 aagaatttga ttccattata ttcaacagga ttgtgagatg cagatataac cacaccacaa
    46261 tcagcaccat attttctagt taaatatgcc actgcaggtg ttggtattat tccaacagta
    46321 ataacatcac atccaacaga cataagtcct gctattaaag atgcttccaa catatcacct
    46381 gatattctag tatccttacc tactactact tttactctgt ggtctccttg agctaagaca
    46441 aatccaccag ctctaccaag tttatatgct aaatcacatg ttagttctgt atttgctact
    46501 cctctaactc catctgttcc aaaatacttt ctcactaaag attctccttt cataattgaa
    46561 aagcttgtaa tttgtattat atttttatag ttcataaaat atcattatag gcttaatttc
    46621 agtcataaaa atattttaat acttatcagc aaaaaagaca atagaaataa ttctattgtc
    46681 ttttttttga catctttttc tttattagtt agttgcttta gaacatgtag catccatacc
    46741 tttttgtaaa gcttctctgt ttaatggaac gaatttttct tttctaggtc cgaaaacttt
    46801 tttgaatgct tctagtacag attctatatc tacagtatca gatactttta agtaagcgcc
    46861 taacattatc atgttagcaa ctttatcatt tcctaattca gctgctaatt catttgctgg
    46921 tatataataa acatctacat ctgttctatc aactttttct tttattaaag aactatttac
    46981 tattattttt ccaccaggaa taacatcatc tttgaatttt tcaaatgctg gtatatttaa
    47041 taatatagct gcagttgcat catctgttat aagtggagaa ccaactggct cattagatac
    47101 agttactgca cagtttgctg tacctccacg catttctggt ccataagatg gtaaccaaga
    47161 aacttctttt ccttctagca ttcctgcata agtaagtaat tgtcccatag acataacacc
    47221 ttggcctccg aagcctgcac atattactct agctgtagac atttacttca cctcctcaac
    47281 ttcaacattt ttgaaatttc ctaatggata gtatggtatc atgttgtctc ttaaccattg
    47341 taaagcatcg tttggagcta gtccccagtt tgtaggacaa gtagataata cttctactat
    47401 tccaaatcct aatcctgctt gttgaacttg gaatgctttc tttatagctt ttttagcttg
    47461 tctaacatga gctggagtat caactgctac tctttctaca aatacagctc ctgttaatgt
    47521 agctaacatt tcacttactc ttattggata accttgagtt tcagcatttc ttcctgattg
    47581 agcagtagta gctctttgtc caactaatgt agttggagcc atttgtccac cagtcatacc
    47641 ataagttgta ttgtttacaa atatagtagt tattttttct cctctagcag ctgcatgaac
    47701 tatttcagca gtaccaattg aagctaagtc tccatcacct tgataagtga atactgtatt
    47761 ttcaggatga actcttttta ttcctgtagc agcagctggc gctctaccat gagcagcttc
    47821 ttgagtgtca cagttaaaat atttatatcc taaaactgaa catccaactg gaacaacacc
    47881 tacagcatca cctaatactc ctagttcttc taatacttca ccaactaatc tatggattat
    47941 accatgtgta catcctggac agtagtgagt ttgagtatct tgtaatcctt cagttttctt
    48001 aaatacaaca gccattatct agcacctcct gcaaccaatt ctccggcaat aattttttta
    48061 gctttttcaa ctatttctgc tggtgttgga gtcattcctc ctggtcttcc atggaagtaa
    48121 actggtaatt taccttctac tgccatttta acatcttcca ccatttgtcc catactcatt
    48181 tcaacagtca atatgtttct agcattagga atttggttga atgcttcgaa tgggaatggc
    48241 cataatactt taggtcttat aagacctgct tttattcctt cttctcttaa tatatcaatt
    48301 gcgcttttta ctactcttga agtagttcca taagctgcaa atacaaattc agcatcttct
    48361 gttttgtaca tttcgtattg tacttcattc ttttccattg catcaaattt ttcttgtaaa
    48421 tgccaacaat ggtcttctag tacttctggc tctaagtata gagagtttat tacattaggt
    48481 tttctttttc ctttagttcc aacagttgcc caatctttag gaggcaattc tcttttcttc
    48541 tcaggagctc taaattcaac tggctccatc atttgaccta tcataccatc tgcaactacc
    48601 ataactggtg atctgtaata atctgcaacg tcaaaagctt ccattatcat atcaacagct
    48661 tcttgaactg tagctggtgc gaatactgga gttctgtagt ctccatttcc tccacctctt
    48721 gtagacataa agtagtcagc ttgtgaaggt tgtatacttc caagtcctgg accacctctc
    48781 attacattaa gtacaacaca aggaacttct gctcctactg catatgttat accttcttgc
    48841 tttaaagcaa ctcctggtga agatgaagaa gtcataactc ttgctcctgc acctgctcca
    48901 ccataaacca tgtttatcgc agaaacttct gattcagctt gaacgaacgc tccacctatt
    48961 ttaggtaact ctcttgataa atattctggt aattcacttt gtggagttat tgggtaaccg
    49021 aagaaatatt tgcaaccagc ttctatagct gcttttccaa atgcttcgtt acctttcata
    49081 agtatcttag ccattaaaat tcccccttaa atattaatta aattaatctc tttctactgt
    49141 tataactaca tctggacaca tagttgcaca acttgcacat cctatgcatt tttccatttc
    49201 aaaaacagtt gctgggttat accctttttt gtttattaca tttgagtcta attgtatgat
    49261 ttttactgga catgcttcaa cacacaatcc acaaccttta caacgttctt gattaaaaga
    49321 tacttttcct ttagccattt acctaagcct ccttaaactt tctatatatt tgagctcaaa
    49381 ctacatccag tcttctctca tatataattt tataggaaaa atttctcctt ctaaatcttt
    49441 tggtaactgt tcaacaagat tttctaaaca tgcaacatat cttatcggta tattacattt
    49501 ttcagaaact tcttttgcaa ctttctgacc tcttaaaaca tcatcaatag tagtaaacct
    49561 aataagatga gtattattaa ttagacctgt tacctttaat ttagaagatt tttctatttc
    49621 ttttatatat tggataactt cttcagaagt ttgagttttc tccctatttg catttattac
    49681 aaaaagcata tcataatcgc cttcttttaa caaatgacta aatcttccta taaccctagc
    49741 acctacatta tctccaccta aatctataac agaattgtat gaatggtcta ccataggaga
    49801 cattacctct gctgatactg ctggtaaatc taatgttgga gcattaattg agctatctat
    49861 tggttgtata cctaaggatt tcattaactc tttcttttct ctagttctaa agtatacatt
    49921 taccacatct aaatcggcta tagcaacttt gccagaaacc atatctctta acttaacaac
    49981 atagttcatc gcaaattcac ttttaccact tccatagtga ccagttatga ttcttattct
    50041 tttatcatta gttatcataa acttgagcct cttcttcacc agttaaaact ctaagtccac
    50101 cttgagctaa tgcaatcatt tcatcttcac ctggataaac ttttacatct gctataaatt
    50161 taactctatc tgcaatcatt tctgtaaaca tttttgaata tgcgattcca ccagttaata
    50221 atattgcttt tacatctccc ttaagaactg cagcactagc tcctatttct ttagagattt
    50281 gatatgccat agcttcatat gctaatttag ctttttcatc accagcttca attctttctt
    50341 caacttctct agcatcatta gtgtttaagt atgcaactaa tccgccatta ccttttattt
    50401 tctttttaat ttcatcttga gtatattttc cactaaagca catttttact aatgcaccta
    50461 ctggtagtcc accacttctt tctggagaga aaggtccttc tccatctaat gcgtttgcaa
    50521 catctactat tttaccattt ttatgagctc caacagaaac tcctccaccc atatgtgcaa
    50581 ctataagatt tatatcttca tatttcttgt ttatttctct agcatatctt cttgctattg
    50641 ccttttgatt taaagcatgt actacacttg ctctacttat ttcaggcata ccagaaattc
    50701 tagcaacatc ttctaattca tctacaacaa cagggtctac tatgtatgaa ggaacattta
    50761 cttcttcacc tatttgtttt gctattattc cacctaggtt tgaagcgtgt tctcctaaaa
    50821 ctcccacttt taaatcttca atcatagcag cacttactga ataagtacca ccttttatag
    50881 gtttaagaag tcctcctcta cctactacag catctaattc agatgttttt actccacctt
    50941 cttttagagc ttcttctatt acttgtttac gaaattcaaa ttggtcagac accttctcat
    51001 attttcctat ttcttctgaa gaatgtctta aagttttttc aaatactaaa tcctcattat
    51061 caaatacagc tattttagtt gatgtcgaac caggatttat tgttaagatt ttaaatattt
    51121 tgctcatttg cctatttccc ccttaaaatt atgcctttgc tgccattaaa acacctaaag
    51181 ctattgagtt tagtttagtt tcttcactgt ctgctctaga agttaatatt attggtgctt
    51241 tagccccaac tataactcct gcattttttg attttgagaa gaataccaaa gctttatata
    51301 atatgttacc accttcaata tctggggcta ataatatatc agctcttcct gctacaggat
    51361 gatttatacc tttatgttta gctgcttcta aagatactgc attatcaatt gcaaaaggtc
    51421 caccaaccat acaacctttg atttctcctc tttcatacat ttcttctagt tctttagctt
    51481 caactgtatc tttcattttt ggatttactt tttcttttgc gcatattgca gcaacttttg
    51541 gttcacttat atctaatgaa tgtgctactg tgcaagcatt ttctatgatt tgctttttag
    51601 tatttgtatc aggagctaag ttcatagctg cgtcagttac gaaaaataat ctatcatatc
    51661 cctctacatc aaatactgct acgtgactta atacatttcc agttctaaga cctacttctt
    51721 tatttaaaac tgcttttagt attattgatg tgtctactaa gcctttcatt accatgtcgg
    51781 cttttccttg tgaaactaat tcaacagatt ttagagatgc ttctgctaaa tcttttatat
    51841 ctatcagttc ataattttcg atatccatgt ctatgctttt tgcaatttct ttagtctttt
    51901 ctatatctcc tactaaaatg gcatttgcta ttttttcttt tctagccatt tcaactgcca
    51961 ttaaaacttc tttatcttgg caacatgcta ctgatatagt tttaggtcct ctttcttttg
    52021 caaacttaat tacttcttca aaacttctca tgcatttcca cctcatcttg aattttttga
    52081 aatagaactt tttatatctc aattaaatta taactcgttc agcaacgttt tcctagagat
    52141 aattgaaata tttaattcaa aatatttatt taaatctatt tatcagtctt ttttcatata
    52201 tctagcgctt tttagattta acaaatatct cattgtcctt attttataat ataactaaaa
    52261 taaacaagat gcaacaaaaa cataaaatta acaaacttaa ttttataggt ttttttgtcg
    52321 catcttgtaa caactaaata ttcgaaatat ttgttcattc cacttcaaca atattaggtt
    52381 ctatatttat ttttttaaat tgatatgttg tttcatattt tattttatat ttactatctt
    52441 cctcaggacc ttgtgataaa tcaatatata atgaaatatc ttttttattt aacttactca
    52501 aatcctccat tgactctaca acaactttta tatctacatc agaaattaca tttggattat
    52561 ccatatttgt tgggatattt cttaattcaa tatctcccgc attatataaa aattctgcac
    52621 tcaattgttt tgtatcattt aatttaattg ttatagagct atcctcaaga gttataccct
    52681 ctggaatttc taagctaact tctttttctt caccattagc caaatcagct aagtcaatag
    52741 gttttgtctt aatataactt attttgtcta tatcttcttt ctttcctttt attgtaactt
    52801 gttccttact taatttatag tttttcagta catcattatc tttattatca tttagactta
    52861 ctcttattgg gacaattttt tccttcaata gttctatctt tgcagtcaca aaggatgaac
    52921 ttaatgttac tccttcaacc ttagttccat cactatttaa tggtattaat tgcaattttt
    52981 tagaaaaatc atctgtctca ttaccaacat ctagattagc tcttacactt ttgacttctt
    53041 taactagagt tcttggacct gatacatcga caaattcctt actaagttca attttattta
    53101 tatttctttt tgaatttcca cttacttcta cctttatatc tcttttttct tttatgtttt
    53161 tttcaagatt tactactaaa acatcatgct taaattctag agtaacacct tgaggaatat
    53221 ttgcttttaa atatacttcc ttctgtcctt gtattgggtc ttcaatggtt ccatatacat
    53281 gtatatcttc ttttttaaca ctttgtacct ttgataaact accttttata tatacatctg
    53341 ctgttaattt ttcgtctgga tatataacaa gatttttatc ctttaaatca ctcatattac
    53401 ttatagatac aggtatatct tcaaaccctt tagtttcttc agggtcaact acagccataa
    53461 catacatcca taatgcaatt gcacttaaaa atgatattat ttttattttg gtattatttt
    53521 ttagtctatt tatcatttaa atataccacc tttgaaaaaa cttcttgttt ctgtatttgt
    53581 ttttaaatta ctacgtaata tattcatcat tctttctcta gaaatatctc tatataactt
    53641 acctgcttta gcaattgata cgccacctgt ttcctctgat acaatcaaag ttatacagtc
    53701 agacacctca cttaccccta ttcctgctct atgtctagta cccaaatctt tacttaaatc
    53761 cttactttct gtcaatggca aaaaacatgc agctgctttt accttagaat ctctaattac
    53821 aacagctcca tcgtgtaatg gtgtatttgg tataaatata tttattaata gttgcctaga
    53881 aatttcagca tcaataatag ttcctgtatt tattatatca cttattctag tttctctctc
    53941 cattatgata agagctccta ttttttgtct agaaagtgaa tataaagctt ctacaatttc
    54001 ttctatattt ctattgagag tttcttcaga agtatttaca ttctttccaa ataaattaaa
    54061 ctttgctctt cctatatgtt caagtcctgc tctaaattct ggttgaaaaa ttataagagc
    54121 agctataact ccataatcta aagctttaag agatatccaa taaacggtat gcaatttaaa
    54181 ggtgttacta agttgagttg ctaataaaag aaatattata cctttaaaca cttgttctgc
    54241 tcttgtatct tttataaaca taaatatttt gtaaaaaata tacgctacta tagatatgtc
    54301 aattaaatca tatatactca ttcttaatat tatattaaaa aatccattta aaaaagaatt
    54361 tatatctaac acagttttat cctcctgttt gataacttct taattattta cactaagtat
    54421 tatactatat aaatctaact ctatacaaac tatttaactt ttatgtaaat aattctagtt
    54481 ttatatttgg agattgttaa ataatctcta aacacattaa aaatatcaac ttaataaata
    54541 catatttctt attaaagtta gtttttactc aaataactca aaccatcaag gcgcgaatac
    54601 aaaatagtac tcgcgcctta attcagtttt atgttctaaa atatcttaat atatctttgt
    54661 ttaatatata ttaattatta taaattctat aatccactta ttaactataa acaaatacaa
    54721 aatctaaata ttacttatca tcaatattat tccattagat tcatttttat attaaagtaa
    54781 taacttattt catatctaga ttagataaat ttattttaat atcttactat aaactatcat
    54841 taaaacttat tatgtataac tagatataat ctcctacagt gaaaataata cacaatacct
    54901 aaagttatat atccatttca cagtataa
//
LOCUS       DAMJZE010000006        54814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJZE010000006 DAMJZE010000000
VERSION     DAMJZE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.006"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            103..945
                     /locus_tag="PC541_000270"
     CDS             103..945
                     /locus_tag="PC541_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2837758.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            960..1424
                     /locus_tag="PC541_000271"
     CDS             960..1424
                     /locus_tag="PC541_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837759.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1411..2583
                     /locus_tag="PC541_000272"
     CDS             1411..2583
                     /locus_tag="PC541_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2837760.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2627..3562
                     /locus_tag="PC541_000273"
     CDS             2627..3562
                     /locus_tag="PC541_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2837761.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3836..6529)
                     /locus_tag="PC541_000274"
     CDS             complement(3836..6529)
                     /locus_tag="PC541_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2837762.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTKVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            6743..7441
                     /gene="yunB"
                     /locus_tag="PC541_000275"
     CDS             6743..7441
                     /gene="yunB"
                     /locus_tag="PC541_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2837763.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7653..8660)
                     /locus_tag="PC541_000276"
     CDS             complement(7653..8660)
                     /locus_tag="PC541_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2837764.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            8888..9772
                     /locus_tag="PC541_000277"
     CDS             8888..9772
                     /locus_tag="PC541_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2837765.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            9974..11524
                     /locus_tag="PC541_000278"
     CDS             9974..11524
                     /locus_tag="PC541_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2837766.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11553..12200
                     /locus_tag="PC541_000279"
     CDS             11553..12200
                     /locus_tag="PC541_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2837767.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12342..12602)
                     /locus_tag="PC541_000280"
     CDS             complement(12342..12602)
                     /locus_tag="PC541_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2837768.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12819..14102
                     /gene="hflX"
                     /locus_tag="PC541_000281"
     CDS             12819..14102
                     /gene="hflX"
                     /locus_tag="PC541_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2837769.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            14110..14787
                     /locus_tag="PC541_000282"
     CDS             14110..14787
                     /locus_tag="PC541_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2837770.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            14873..15316
                     /locus_tag="PC541_000283"
     CDS             14873..15316
                     /locus_tag="PC541_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2837771.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15621..16265
                     /locus_tag="PC541_000284"
     CDS             15621..16265
                     /locus_tag="PC541_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2837772.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16351..16728)
                     /locus_tag="PC541_000285"
     CDS             complement(16351..16728)
                     /locus_tag="PC541_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2837773.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16728..17114)
                     /locus_tag="PC541_000286"
     CDS             complement(16728..17114)
                     /locus_tag="PC541_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2837774.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17293..18048
                     /gene="nadE"
                     /locus_tag="PC541_000287"
     CDS             17293..18048
                     /gene="nadE"
                     /locus_tag="PC541_000287"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2837775.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18131..18850
                     /locus_tag="PC541_000288"
     CDS             18131..18850
                     /locus_tag="PC541_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2837776.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(19017..20021)
                     /locus_tag="PC541_000289"
     CDS             complement(19017..20021)
                     /locus_tag="PC541_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2837777.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            20287..21183
                     /locus_tag="PC541_000290"
     CDS             20287..21183
                     /locus_tag="PC541_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2837778.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            21304..22566
                     /locus_tag="PC541_000291"
     CDS             21304..22566
                     /locus_tag="PC541_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837779.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            22642..23835
                     /locus_tag="PC541_000292"
     CDS             22642..23835
                     /locus_tag="PC541_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2837780.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            24464..25258
                     /locus_tag="PC541_000293"
     CDS             24464..25258
                     /locus_tag="PC541_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2837781.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            25353..26693
                     /locus_tag="PC541_000294"
     CDS             25353..26693
                     /locus_tag="PC541_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2837782.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            26830..28062
                     /locus_tag="PC541_000295"
     CDS             26830..28062
                     /locus_tag="PC541_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2837783.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            28102..29244
                     /locus_tag="PC541_000296"
     CDS             28102..29244
                     /locus_tag="PC541_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2837784.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            29255..30049
                     /locus_tag="PC541_000297"
     CDS             29255..30049
                     /locus_tag="PC541_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2837785.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            30060..31082
                     /locus_tag="PC541_000298"
     CDS             30060..31082
                     /locus_tag="PC541_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2837786.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            31181..32557
                     /locus_tag="PC541_000299"
     CDS             31181..32557
                     /locus_tag="PC541_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2837787.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(32889..33233)
                     /locus_tag="PC541_000300"
     CDS             complement(32889..33233)
                     /locus_tag="PC541_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837788.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            33721..34731
                     /locus_tag="PC541_000301"
     CDS             33721..34731
                     /locus_tag="PC541_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2837789.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            34867..35355
                     /locus_tag="PC541_000302"
     CDS             34867..35355
                     /locus_tag="PC541_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2837790.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            35531..37459
                     /locus_tag="PC541_000303"
     CDS             35531..37459
                     /locus_tag="PC541_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2837791.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            37636..38046
                     /locus_tag="PC541_000304"
     CDS             37636..38046
                     /locus_tag="PC541_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2837792.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            38068..38370
                     /locus_tag="PC541_000305"
     CDS             38068..38370
                     /locus_tag="PC541_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2837793.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(38587..39279)
                     /locus_tag="PC541_000306"
     CDS             complement(38587..39279)
                     /locus_tag="PC541_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2837794.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(39305..40492)
                     /locus_tag="PC541_000307"
     CDS             complement(39305..40492)
                     /locus_tag="PC541_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2837795.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            40828..42705
                     /locus_tag="PC541_000308"
     CDS             40828..42705
                     /locus_tag="PC541_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2837796.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            43062..44468
                     /locus_tag="PC541_000309"
     CDS             43062..44468
                     /locus_tag="PC541_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2837797.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            44496..45344
                     /locus_tag="PC541_000310"
     CDS             44496..45344
                     /locus_tag="PC541_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2837798.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            46005..46697
                     /locus_tag="PC541_000311"
     CDS             46005..46697
                     /locus_tag="PC541_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2837799.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            46694..47629
                     /locus_tag="PC541_000312"
     CDS             46694..47629
                     /locus_tag="PC541_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2837800.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            47777..48700
                     /locus_tag="PC541_000313"
     CDS             47777..48700
                     /locus_tag="PC541_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2837801.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            48684..49454
                     /locus_tag="PC541_000314"
     CDS             48684..49454
                     /locus_tag="PC541_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2837802.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            49464..50246
                     /locus_tag="PC541_000315"
     CDS             49464..50246
                     /locus_tag="PC541_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2837803.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            50623..51159
                     /locus_tag="PC541_000316"
     CDS             50623..51159
                     /locus_tag="PC541_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2837804.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            51191..51580
                     /locus_tag="PC541_000317"
     CDS             51191..51580
                     /locus_tag="PC541_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2837805.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            51617..52003
                     /locus_tag="PC541_000318"
     CDS             51617..52003
                     /locus_tag="PC541_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2837806.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            52130..53572
                     /locus_tag="PC541_000319"
     CDS             52130..53572
                     /locus_tag="PC541_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2837807.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            53869..54684
                     /locus_tag="PC541_000320"
     CDS             53869..54684
                     /locus_tag="PC541_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2837808.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        20291 a         6588 c        10053 g        17882 t
ORIGIN      
        1 atgaataaat atatattata cattatcaat gataggttat ataataaaat cactaacaat
       61 tatattataa gaaaattata aaaattacaa ggaggtatag aaatgaaaga aaaagcaggg
      121 attagaaatg ccataatagc agcctttata atcacaatat tggcagaatt aattggacct
      181 atttcattta aagttatggg aattaatgta actttactta gtatactatg ggctatattc
      241 ataggaatgg ctgtttctcc tcatttatta gggagagtta taccagcctt aaaaaagttt
      301 ataggggata atgaaataaa tgtatctcca tatttattat ctttgacact atatccatta
      361 gggataatgt ttggaattaa tgcagggcct aaagttggta tattacttca agcaggacca
      421 gcacttttat tacaagaatt tggtcatatg ggtacaatgt tgatagcatt acctgttgga
      481 gtagcattag gtcttggaag aagtgcatta ggtggtactt tttcattatg tcgtgacaca
      541 gcactaggaa ttattggaga taagtatgga ttaaactcac cagaaggtat aggaacacta
      601 ggaacttaca taagtggtag tatatttgga acattattat tttcattttt agcaccagtg
      661 ggtgtacttt taggatttca tccatatgct ttagcaatgg catcaggaat gggtagtggt
      721 tcaatgatgg gagctgcaac agcatcatta atgaatacag ttccacaaat gtcagagcag
      781 attttagcat attcagctac tagtggattg attacagctg ttactggaat atatatagaa
      841 ttattcttat ctttaccagt agccaactac tattatagca aagtagctcc agttattgaa
      901 agatttagag gaagaaaaac aagaagaaca agtaataaat tgtaaaggag gatatatata
      961 tggaaaaaac ttttaagact attatggagc agataaaaat agtattgttt atatatatta
     1021 ttatttgctt tggacaaatg atagcactta agactcaggc tcaagatttg ttgattggaa
     1081 gtgtatctgg atttttgttg gtgataattg ctattataat aaagaatttt gttaaaaaac
     1141 ctaatttacc aggttttgca tgggctacat tagttgcatt tgttttatcg cttccaatat
     1201 ctccagtggg ggatatcata gcaacaaatg tagggaaaat caattttatg tctacagtaa
     1261 caccattatt agcttttgca ggaatctctg taggtaatca attagatata ttaaagactt
     1321 tgagttggaa gttagtagtt atatcattta tagttatgac atctacttac tttggttctg
     1381 cttttatatc tcaaacagtt ttaagattaa atggaatgct ttaaggaggt agtaaatatg
     1441 aatgaattaa aagcaaaaat taaagaggaa ataaagttat tatctgaaga gaaaaagaca
     1501 tcatttgaaa aagtttctga ctatttattt tcaaatccag aattagcatt tgaggagtat
     1561 aagtcacaaa aagcattatg tgatttgtta gaagaaaatg aatttaatgt aactaaaggt
     1621 gtaggaggat tagaaacatc ttttgaagct gtgtattcaa atggaactaa tgggaaaaca
     1681 gttgcttttt tagctgaata tgatgcactt ccaggaatgg gacatgcatg tgggcacaat
     1741 atcataggga caagttctgt tggtgcaggt atcatattaa aagaaatcat gaaaaaacat
     1801 aatatagaag ggactgtaaa ggtttttgga acacctgcag aagaaagagt aggtggaaaa
     1861 ataactatga ttaaagaagg tgtatttaat aatgtagatg ctgcattaat tttacatcca
     1921 agtgatgctt caatgcctga tgatatatca tttgctcaag ttaatttaaa atttgatttt
     1981 actggtaagg catcacatgc tgctgctttt ccttgggaag ggaaaagtgc cttaagtgga
     2041 gtaatagcac tatttaacag tgtaaatagc atgagattac atcttaaaga ttatgcaaga
     2101 gtacatggaa ttataactga tggaggtagc atacataata taattcctga aaaatctaca
     2161 gctatattta atgtaagagc cttaagtata gaatatttaa atgagatttg tgaaatgctt
     2221 aaaaattgtg ctaaaggagc tgcaatttct actggaacta atgtagaaat agttcaatta
     2281 gatgaaatat acaaagagat taagaatgat tctgaattag tgaatatagt tagacaaaat
     2341 tttgaagttt tgggtgaaga ctatgttgaa agagatttaa gtcaaggtat aggttcaact
     2401 gatacaggaa atttaactca tgagatacct gcaatacaag catatataaa attaaaagaa
     2461 aatactgcaa ctcatacaga tgaatttgct gttgcagctg gtggtgaaga aggcagagta
     2521 gcacttataa aagcaattaa ggtgttagca atgtgtggag tagatatttt gtatagtaaa
     2581 taatatataa ttttaaaata tattaagaaa ggaaatcgga ttagacatga tattaaaaca
     2641 agtaatagag gtttatgaca ttttagataa agcaaatgct aatggggaag aagtaaaaac
     2701 ttacttacaa ggttatggtg aagtagatgt aactgtaaaa gaattgtctt caagtaaagg
     2761 ttcaacagat ttagtaaagc ttacaattcc tggaaaaaat ggaaaattaa aaggtggaga
     2821 tgctcctact cttggtattt taggtagact aggagggatt ggagcaagac cagaagttat
     2881 aggttttgtc tcagatggtg atggagcatt ggtagcaatt gcagtggctg ctaaactttt
     2941 ggatatgcaa cgaaaagggg atatattaga tggtgatgtt gtaatttcaa ctcatatttg
     3001 cccagatgct ccaactaaag aacactatcc tactcctttt atggattctc caattgatat
     3061 gatgactatg aatgaaaatg aagttgatag tagttgtgat gcaatacttt ctatagatac
     3121 tactaaagga aatagagtaa tcaatactag aggatttgca atatcgccaa ctgttaaaga
     3181 aggctacata ttaaaaacta gtgataacct tttagatatt atgcagactg taacagggaa
     3241 atctccattt gtattccctt tgtctattca agatataaca ccatatggaa ataatctgta
     3301 tcacttaaat agtattttac aaccagcagt agctacagat gcaccaatag taggagtagc
     3361 aattacaaca gagatgcctg ttgctggatg tgctacaggt gcaactaatt ttagtgattt
     3421 agattcagct gggagatttg caattgaagt tgccaagcta tttggtagaa ataagtgtga
     3481 tttctatgat aaagaagagt gggaaatgct tattaagaga tatggcaaat taaatagatt
     3541 tcaaacattt ggtatacagt aattagaaaa atattaagtt tatataattt gtgatataac
     3601 tatttaagaa tatataaaca attaggaata aaagtaagaa taaagcactt agttaaaaac
     3661 tttaaaaata aaatatttat ttgtttatat aaaaaggagg attctggtta ttgggattct
     3721 cttttttatg aatacaaata atatattgta attataaaaa aaggagcgtt tcaaagtgat
     3781 ttttttaatc accttgagac aactccttat atttttattt aaattctatt ttttattaag
     3841 ttgctggagt tccaccacca tcaggaggag tagtagagcc accaccatta tttccaccac
     3901 catcaggagg agtagtagag ccaccaccat tgtttccgcc attatcagga ggagtagtag
     3961 agccaccacc attatttccg ccattatcag gaggagtagt agagccacca ccattgtttc
     4021 cgccattatc aggaggtgta gaaccgccac cattatcacc tgtgttatta ttgttattat
     4081 tattttcttc tggttggtta ttgtctggtt tttttgtatc ttcagatgat ggttgagaac
     4141 ttactttagt aggagcagta ccttcaataa atgctgcttt agagccagaa gattgctttc
     4201 ctccatcctc taaattaatt tttgtaaaat atacattttt aggaacttca aattcagtaa
     4261 ccgttaaatt agcatgtatt ttttccataa ttttttccca tagttttgca gtactagtac
     4321 ttccatgtgg tacttctcta cgttttatag tgttaccaga atcatctttt ctaccagcat
     4381 catctccaat ataagttgca ccaacataat aaggagtgta accgacaaac caagcatcat
     4441 atgcactatt tgtagtacct gttttacctg ctacaggcat tccctttgga agtgtagcaa
     4501 caccaccagt accttcagta attacgcttt ctaacatatc tgtaacaaca taagctactt
     4561 cagggtctac aactttatgt tcttcaggag catttttaac taataattga ccatcaaagg
     4621 tagttatagt agtaaaaata gaaggttcta cataaatacc gtgatttgcc aatgtaccat
     4681 aagcagcagc catttgaagt ggagtaatac cattcgccat accacctagt gtcaatgatg
     4741 ggaattttct atcacttgct tcagatgaac ctgagtgatt attctttaaa gttgttatac
     4801 caaagttctt taagtaatcc atcattatat ctacacattc atcataagag tctccaagca
     4861 tttcagcagt tttgacagct atcgtgtttg aagatttagc aagtgcttta cgtaaactca
     4921 tagaaccatg accagcagta gtagttcttg gattccattt attattttct tcaaacttat
     4981 atcctcctcg agaatcactt agagcagttg cttgtgttat ttttagagta tcaatagctg
     5041 gcgtatatat tgctaaaggt tttatagaag aacctggctg atgagggtta gtagcacgat
     5101 ttaaagtttt tctaccactt atatttcttc ctccagctag tgctcttatt tcaccatttt
     5161 tataatctaa gattaccatt gcagcttgcg gttggctaac tccatcagga ccgaccatat
     5221 gtcctgggaa attgctattt ctatcaaatt cttcttctaa agcatcttgc attttagggt
     5281 ctatagttgt gtttacaatt agaccaccat tgtagaataa gttttgagct tcatcatttg
     5341 tatgtccttg ttccattaaa gcatttataa cttcactttc gataaggtct tctacagatg
     5401 aagaaacttt atctgatgat tgaggcaatt ttatttctat tttagctttg attgcttcat
     5461 catattcact ttgttttata tatttcaatt ctaacatttt ctttaaaaca atttcttgtc
     5521 tttttttagc ttcaggatta cttacagcct ttctaacaac taatgtacct gcttttaatt
     5581 gtctataagt atcattagat ataagctccc aagattttat tttttcaatc atatcaaagt
     5641 ccactttagt agggtcatct aaatcatcat cagttgtgtt aacaaaaaat aataatttat
     5701 tctctaaatc ctccttagtt tcatttccct ctaacttaga agttttatag gctgaaaatc
     5761 ttgatggatt tttagttgaa ccagcaagaa gtgctgactc tgctagagta aggtcagctg
     5821 cagacttatc aaaataacca cgtgcaccag cctcagcacc tgctagacct ctaccaacga
     5881 agaagttatt taaataagtt tctagtattt tatctttact tactgtttta ctcatctcat
     5941 gtgcatagta aatatccttt attttacgag gtatagtttg ctgttcagtt gttaacagca
     6001 ttttggatac ctgcatagga atagtactac caccttgctt agtacctgta agggtcttta
     6061 atactgacct aaataaacct ttaatatcga cacccttatg ctcataaaaa cgctcatctt
     6121 ctattgcaat tacagcatgt tgaagataag gggaaatctt atctaatgga acgggtgtct
     6181 ttttatttac acttggagct gtagataatg tttttccatt agcgtattta atagtagttg
     6241 tttggttcgt ctgtttatct aaaacagctt ctgtaactgg agttacatct cttagtgacg
     6301 caaatactag accagcagta cctgctgtac caacaactag taaaactaaa aatactatcc
     6361 caaatactct tagtttttta aatttatcgc tttttttaga ttttttcttg ttttttgttg
     6421 aacgagtctt gttagctgaa gttctattaa ttggtttatt tgtattacca gatgagctaa
     6481 ctttttttct cctgatttta tttccatttt tatcattatc gttattcata agattttaac
     6541 ctccttactt aatgcaatat tataacacaa ataggagtag ttttgtaatt aatagaaaaa
     6601 atgaatttgc tataatattc tgtacattgt atatgtataa acgtaatata ttaaaaagtc
     6661 tatattttta ttttatttat ttttgacaaa tattataaaa attatgccaa agctacatat
     6721 aaataaatag gaggggataa ttttgaatag aatagtagat agaaaaacaa aaaatgaatt
     6781 tagaaaaatc atagctattt ttttagtaat tcttttcttg tctgtattta taggcagttt
     6841 tatatatata gataaaactt taagaccgac aataacagtt ttagcagaga ctaaagctct
     6901 tgaactagca aataggtcga taaataaagc tgttgctgag atggtagaag gaaaaataaa
     6961 ttatgaagat ttaatggata tacaacttga taataatggt aaaataacta tgatacaagc
     7021 aaacactata atgatgaatg aaattgcatc tgcaatagct ttagaaatac aagatgaatt
     7081 aaaaaaggat aagacagcaa gttcttacat acctatagga acagcattag gaagtcctat
     7141 attagctaaa tacggtccca aacttgaagt atctattgaa cctataggaa cagtatcagt
     7201 aaactttaaa acggaatttg agtcttcagg tataaatcaa actagacata gaatatattt
     7261 agaagctcaa acacaagtta aagtagtaat accacttata acttcaacaa aacaaattaa
     7321 agctcaaata cctatttgtg aaactataat agtaggtgat gttccagaaa gttatgtaaa
     7381 tattccagaa aaaaatctag gaaatgtttt acctaacact ggaaagaata ccaacaagta
     7441 atgtaaagtt gtatattttt aaataaattc aaaataatag tttataaata aaatttttgg
     7501 aaataatatt aatataattt taataaaaat ttatatttta tactatgtaa aatatctaat
     7561 atatttggtc tgtaggttaa ataaaagtac ctttagacca aatatattat taataactta
     7621 ataacatctt aaaacttatt tatttatatt atttacaaca tccatttaat aaatacacca
     7681 taacctacag ctgggtcgtt ttcaattgca tgtgacactg ctccgattat tttattatct
     7741 tgaactattg gagtaccact cataccttgt acaataccac cagtttgagc taaaagcttt
     7801 ttatctgtaa ttttaatttt aaaactttta ctttctggat gtttttggtt ttctatatct
     7861 ataatttcaa tattatattt tttacactta ttaaagttat cttgtaaaat tatttgagct
     7921 tttcctaatt taacttcatc taaagatgct actttatatt tttttaactt ggaaatatca
     7981 aagttattaa tttgacctct tattccaaaa tcagtatttt ctgtaaagct accaataata
     8041 ttttctcgtt ttgcatttat acatccaaca ctacctttat aagatttttc tatattaagg
     8101 tgcttagttg tagaaattga acctgtttta ataggtattt ttcttgcatt accaacactt
     8161 attggatgac ctacagctcc aaactctgat gtatctggat tgatatacgt taaagtagca
     8221 aatccagaaa gtaaattgtt aaaactaatt ttttctaata tgtgtttggt agttttcatt
     8281 gttataagtt gtccacctct atttaataaa atagatactt gtttttcttc tggtaaagca
     8341 tttaaggtat tggcaaagtc actatatcca tcaacttcag cattatttac ctttactata
     8401 gcatcaccca atctaaatgg agatgcttct ccaggatttc taactatgat attttttaat
     8461 tctgcgtcaa tttgaagtac gtttccaatt ggtattaaat attcaggttc atttttttgt
     8521 aaattttgtg cataaattaa attatttgag aaaaaatata ataaaaataa aaatgttaaa
     8581 acaattgtaa attttgactt agctaagttg gtttttttac ttaaagtatt atttttaaaa
     8641 tgaaaatttt taagttgcat aaaacacctc tttcttatta aattatttta ataatagaat
     8701 tgaagttttc ttgctgcttc tatattatgt tgtgcaagaa tcatgttttg atgcttggaa
     8761 aactagctca ataacacctt agttttcata aaaataaata aaataaatag tgtatatgat
     8821 aaaataaata catattcata taaataaaaa taaacaataa ctgtctagca taagaaagga
     8881 gcaaaaaatg aaaaaatata gattgttagt tacttgtata attgccatgg gaatcataat
     8941 tataggaaca tcaaatccag ttagcaattt ttacaagact acaaatttga ttagttcagg
     9001 aaatatcaca aatgaaaaag cagataaaga tgaaaattca aacaaaaata ataaagataa
     9061 aaatgaagac aaatctaatt ctaaaagtaa taaagataag tcatcagagg acaaatcgtc
     9121 agataataaa aatgaaattc aaaaaaataa aaaactttta atttgtatag acccagggca
     9181 tcaaggaaaa ggagatagca atttagaacc agtagctcca ggttcttcct ctaaaaaagc
     9241 gagagtatct tcaggaacag agggtattgc tacaaaaaag ccagaatatg ttttaaactt
     9301 agaagcatct cttgttttaa agagtatatt agaatctaaa ggttataatg taataatgac
     9361 tagagagaca catgatgtaa atataagtaa ttcagaaaga gcaatacttg ctaatgacaa
     9421 aaaagcagat atggttgtaa gaatacatgc agatagttta aataattctt caaaaactgg
     9481 agcatctata ttgattcctg aaaaagatgg aaaatataca gctcctatat atgaagaaag
     9541 taataaatgt gctgaattta tcaaacagaa tatggaacaa tcaggtatac aaatcaatgg
     9601 aatcgttcaa agaggtgatt tgactggatt caactggtca aaagtaccag ctgtattagt
     9661 agaaatggga tttatgagta actataatga agaccaaatg atgtcaaatc cagactacca
     9721 aagaaaaatg atgcagtgta tagctgatgg attggatgcg tattttaaat aagatatgaa
     9781 tagagaaaaa gttaggttgg tattatttgc ctaattttta tctctatttt ttattgtaca
     9841 taacacaaag aagaagttta ttttaggtta aattgtcgta aatataacta aaaatattgg
     9901 gaatacttga tttaagttta aaaagaatat attatagtat agaatgtgta aaattttttt
     9961 gagaggtgtt gaaatgctag tagtagaaaa tgtgagtcat ggatttggtg cgagaacaat
    10021 attagaaaat gtatccttta gacttagaaa aggtgaacat attgcactag ttggtgctaa
    10081 tggagaagga aaatcgagtt ttttaaatat aataacaaag aagcttatgc cagatgctgg
    10141 aaatataaaa tggtcttcaa gagctacagt aggttactta gaccaacata cggtattaag
    10201 caagggaaaa actataagag aagtgcttag agaggctttt aagcatatgt ttgacttaga
    10261 gcaagaaatg attgctatgt atgacaaaat gggagaagct agtgatgatg aaatgagtaa
    10321 gcttcttgaa gaaacagcag agatacaaac tatacttgaa aatagtggtt tttatatgat
    10381 agatgcaaag atacaagaag ttgcaaatgg tcttggtctt ggagaaattg gtcttgataa
    10441 agatgtaact gacttaagtg gagggcaaag aacaaaagta cttcttacta aacttctact
    10501 tgaaaatcca actattctta tattggatga gcctacaaac tatttagacg aagagcatat
    10561 aacatggctt actaagtacc ttcaagagta tgagaatagt tttgtcttag tttctcatga
    10621 tattgaattt ataaataata catgtaatgt aatatatcat atggaaaatg gagagttaaa
    10681 tagatataaa ggaaattatg atgaatttgt aagacttaat gatataaaga aacgtcaaga
    10741 agaacaagct tatgataaac aagttgaaga aagaaaaaga ctagaggatt ttgtagctag
    10801 aaataaagct agagtagcaa ctagaggaat ggctaacagt aggcaaaagc aacttgataa
    10861 gatggaaata ttagaaagac caaaagaaaa aataaaacct acttttgctt ttaaagatgc
    10921 tagagcagct agtaaaataa tttttgaaac agaaaattta gttttagggt atgatgaagc
    10981 attgacaaaa ccacttaatt tccatttaga aagaggaaaa aagattgcct taaaaggaat
    11041 gaatggtatt ggaaaatcta ccctattaaa aactttactt ggaataataa aaccatttga
    11101 aggaaatgtt aaattaggtg actatttgga agttggatat tttgaacaag agagttcaag
    11161 agaaaatagt aatacaccta tggatgaagt ttggagtgaa tttccagggc ttacaaactt
    11221 tgaagttaga caggctcttg caaaatgtgg acttacaaat gaacatataa cgagtcaaat
    11281 gagagtatta agtggtggag aagctgctaa ggttagactt tgcaaagtaa tgttaaaaaa
    11341 tataaatttc ttagtattag atgagcctac aaatcattta gatgttgagg caaaagatga
    11401 attgaagaag gctatcaaag agtttaaggg aacagttctt ttagtatgtc atgagccaga
    11461 attttattca gaaattgttg atgatgtatg gaatatagaa gattttacaa ctaaaatcgt
    11521 gtagtgattt aacttattgc tggggggatt tcatgaaaaa gtttctaaat gtactttttt
    11581 caatttgtat tagtattgtt ataatagtag gtgttattaa ttttactgtt ggatttaaac
    11641 aactttatta ttttgacatt gattacttaa atatatcaga gttatctggt ttatctaaag
    11701 atgatataaa attgaactac gattatctaa ttgattataa cttaaataag aatgtgtctg
    11761 agttcaaatt accaacatta aaatcttctc cacaagggaa gatacatttt gaagaagtca
    11821 ggaatatatt tcaaaatata aataaattag ctaaattatt attggtagtt tctttagtag
    11881 gaattatatt gagtgtaaaa aataaaaata taaaaatctt aaaaactaca tcaataacac
    11941 ttataattat gccattatta ttaacagttc caattttatt aaattttgaa aagtcattta
    12001 ttatttttca taaattatta tttagaaatg attattggat atttaatccc gatttagacc
    12061 cagttataaa tatattgcca gaggaatttt tttttcactc tggaatgatg attttaatat
    12121 taattttgtt agtaagtata gtactgtttg taatgtataa attatgtaaa tctttatcaa
    12181 taaaaatatt tcaatgttaa atagagggat atttttattt ccaaaataag ataataaaaa
    12241 aggattagat taaaagttat acaataaaaa tagctgtgca ataatattta gactatgaaa
    12301 gtagttgaaa aatatttatg cacagctatt tataatagaa tttacttttt actagtgatt
    12361 ttaactaaat cttgaaattt actcataatg taattgtaat tatctttttt atgaggtaat
    12421 atacaactac tgcaatcttt gataccttta tctgtatacc taaagtttcc accacagttt
    12481 tctcctaaag catacaaagg acaatagcaa aatagacagt taaactcttc tggtttatta
    12541 gttttatgac aagggaaaaa ttcgcaatct ttgtgattaa aaaacttata attttcgccc
    12601 atattttcct cctaattaaa taaaatttaa attttttata ctatataaga ttttggtgat
    12661 aatataatta tactaaataa ttttatgaat agtcgataat atagtattat taatcatagt
    12721 attaatggta taattagtca acagaaaaat aatgtaaaaa taatgtaagt gtattttgta
    12781 attagaatat gttttataac aaaaaatgag gtgattaagt gaaagaaatt caagaaaaag
    12841 cacttcttgt aggtctgaat ttgactacaa tagtgaaaaa aaatgatgat atagacacta
    12901 atgaatctat ggaagaatta aaagaactaa caaaagctgc tggggcagaa gttgtaggta
    12961 gcttaattca aaataaacac tctgtagatg cagcttatta tataggaaaa ggtaaagtag
    13021 aagaaataag agcctatagt gactctttag atgctacttt agtaattttt aatgatgaat
    13081 tatctggtgc tcaaatcaga aatatagaga atgttgttgg aagaaaagta atagatagaa
    13141 ctactttaat tttagatata tttgctcaaa gggcacttag caaagaaggt aaattacaag
    13201 ttgaattagc acaattaaaa tatagattgc cacgacttta tggaatgggt ggtgagatga
    13261 gtagaacagg tgcagggatt ggtactagag gtcctggaga acaaaaacta gaaatagata
    13321 agagacatat acttaataaa gcagcagata taagacgtga acttaaagaa gtaaaaaaga
    13381 atagagagac acagagagtt aagcgtttaa agtcaaatat accgattgta gcacttgttg
    13441 ggtatacaaa tgcaggaaaa tctaccctgt tgaacgaatt aataaagaca cacaaggatt
    13501 atgaacaaga aaaagaagtt tttgtaaaag atatgctttt tgcaacttta gatgtaactt
    13561 tgagaaaggc tcttttgccg aataaaaaag aatttttagt agtagatact gttggatttg
    13621 taagtaaact tccccatgat ttagtagaag cgtttaaggc aacattggaa gaagtacagt
    13681 atgcaaattt aatcttacat gtaattgatg caacaaatac tagttatgaa ttgcaaaaaa
    13741 gtacgactga aggtgtatta aaagaacttg gtgtaaatga taaaaaacat atacttgtat
    13801 ataataaagt ggacaagcta gaattggata tataccctaa aagtcaagaa gatattgtgt
    13861 atatttctgc aaaacaaggc ataaatatgg ataagttatt aaatatgata gaaattgctt
    13921 taatggaaaa tacttattca gtaagtttga tgttacctta tgagagaggt gatatattta
    13981 gtagaataaa agataaatat aatgtggaga attttgagta tggggaaagt ggtataacgc
    14041 tagatgcaga tttagatgaa gaagatttta atatctatag agagtacata ctagaaaaat
    14101 aggttgaata tggatataaa ctgggaaaat atagataatc ttgaagatta ttttataacc
    14161 tatcttcttt ataaagaatc taaaacagta tctcaaatta gtaaaattag aaatatttca
    14221 agtactgaag caagtgagca acttatacaa gcaaagttaa aaataaagga aatgcaaaaa
    14281 gatgattttg aggcatctaa ggatattttg gataaatttc tagaactaga taaaattaag
    14341 agacttgatt ttatggatag cttagatgac gaaaaaatgg tatactttaa gagaaaggtt
    14401 ttcaaaagaa tccttgtaga aaaaaatgct gaagatttaa ttgttttaat ttgggcgaca
    14461 ggtgagctaa aagatgatag gtttttgaaa ttgcttcatc agctcacaaa ccacagacac
    14521 tcagatatta gaagaataac ttattcagca atcagaaaga ttgaatcacc aagtagtaga
    14581 gaagtactgc aaaaaggtct ctacgacaaa aatgcacaga ctaggcagta ctgtgcaaaa
    14641 gctctttcaa aattgggaga tgaaaacagt ttaaagattt tacagcagtt gaaagacaag
    14701 aataagaatt ttgaaaaaga atatgtgctt agagcttatg acgaagccat taagtcattg
    14761 gaaaatgcaa aaaatttgaa aaagtaattt tttattaaag caatttgttt acttaatgat
    14821 gtatttctaa agtttaacaa ggtaataaat aaattaatgg gaggggtttc aaatgatagt
    14881 aagacgttgt gcaggtggag tagtgtttta tgctaataaa gtacttattg taaaaaatga
    14941 taggggtgaa tggacacttc caaaaggaaa gatactagga gggggacttc catatgagag
    15001 tgctgttcaa agagttaaag tagaaactgg catagatgct aaaatgatag atgtggctgg
    15061 cgatactatg tatgaatttt tctcaagaag tagacaacaa gaagtttgta atgcaataat
    15121 gtggtatgtt atggaagctt gtaacactga ttgtgtactt gctcctgaat ttcaagaagg
    15181 tggattttat aaagtaaagg atgctttaga aatgttatca catcataaag agcaagcttt
    15241 agtagaagta tcttataaaa agtttaagga attaaagaaa ttagtttcag agggcgatga
    15301 tgttccagca aattaattgt gaatttagag gattctgagg aagggatagc cccttccttt
    15361 tttaattgaa gcagttttaa gcgtaaatta tatagtctct ttaaactatg atttatcaat
    15421 ttagagattt agtagtttat atttaaaatc agtaagatat atttaaaata aaaattaaaa
    15481 agtatacaat tttattatat aattgtctta tgatgttgat taaagaagta ttaaaagtcc
    15541 atttattttt aaatagaatg attttataaa tatatatttg atgtgtatat tagaaaatga
    15601 ttaaaatagg aggaaataca atgagcaatt acaggacatt acatgaattt ggaacggatg
    15661 aaataacgat tgaaaagtct gtttttattg gatatgctaa gcctataaag agtgaagaag
    15721 aagcattaga gtttataaat gaaataaaga aaaagcataa agatgcaact cataatgtgt
    15781 gggcttatac tgtaggtaaa aatatgaata tacaaagata tagtgatgat ggagaaccac
    15841 aaggaacagc tgggatacct acattagagg ttataaaaaa agaagatttg agagatgtag
    15901 cggttgttgt tactaggtat tttggaggaa caaaattagg tgcaggtggt ctagttagag
    15961 cttatacaaa aggtgctaaa ctgggacttg aagcaggtaa gataatttat aaggtaatgt
    16021 atcaagaagt taaggttaag atagattata ctcaacttgg aaaagtgcaa aatgagttaa
    16081 tgaatttggg gtattttata aaagatactg tatatgagga taatgttgaa atagtagtat
    16141 attcaagatt agaagatgta gagaaattat cagaaaaaat gattgatata acaagtggaa
    16201 caggaaaaat agttttagga gaggaatttt atttgtctga acaaaatgga gaaattttaa
    16261 tataatctaa aaggttatta gaatagttaa tacctcaaat agccaattag tatttttaag
    16321 tgaaaagcca ttaaattgat ttaatctaaa tcaatttaat ggctttttat cggattttgc
    16381 acagaatcct ttttttactt caaataaacc gactaaaagt aaaaatatcc caaatatttt
    16441 ttttaatata tctgaagaca gataattagc taatagtgaa ccaaatacag ctcctaaaac
    16501 cccaaacaca gctataggta taaccaattt aaaaattaca tttttatttt ttatatgtat
    16561 agcaagtgct ataagtgtca tagggataga tgaaagtaaa tttacgcttt gtgctatttt
    16621 agtgtctatg ccagcaagta aaacgagtgc aggtataagg attgttcctc cacccattcc
    16681 cattcctcca ataattcctg caaaaaatcc aattagagca aataacatta aaacaccatc
    16741 cttaatgctg caattatcat aattattcca aaagaaattc taagaaactt tccagtaacc
    16801 ttatttagta atttagctcc tataatacca ccaagaatac ttccaactgc aactttaatt
    16861 gttaagttaa catcataagt acctttagat acatataaaa cagaacttgc tgtcgttaag
    16921 aatattatta tagaaagagc agttgcatga gatttatgtt cttctacctt aacgatgtca
    16981 tttaatattg gaactaaaag agtacctcct ccagaaccaa aaactccatt tataaatcct
    17041 gtaaaaactc caattatcgt attttttaaa ttaaaatcaa agtatttttt ctttaaatca
    17101 tcagtttttt tcataaaaat caccttctga aatagtttta ccaaaatagg caaattatat
    17161 ttatataaaa taagattaaa tataatcttt catgtttgtt taattttgca taaaaaaaaa
    17221 catattaaaa caacatatta atatatggta taatatatta aaacaaagta gcaaatttga
    17281 gaggagataa caatgtcaaa tataaaaata caaatagaca aaactgtaga atggcttata
    17341 aataaggtta atgaggcaaa tgcaaaagga ttaattgttg gtgtatctgg aggaattgat
    17401 tcggcagtag ttgctaattt aattaaaaag gcatttccag aaaactctat gggagttata
    17461 atgagcataa aaagtaatcc tcaagataga gaagatgcac ttaaggttat agagggatgt
    17521 gatatagaat accttgattt agaccttata gaaccacaaa gtgctatatt agacatggta
    17581 gtaggaaatt taaaagataa acatttatat agagaagaat atttaaagat gacagatgca
    17641 aacttaagag caagagtaag aatgagtaca atttatacaa ttgctaacaa tctaggatat
    17701 ctggttgttg gtacagacaa tgctgcagaa atacacacag gatattttac taaatttggt
    17761 gatggtggag ttgatatttt acctattgca aatttaacta aaggagaggt gtatgaatgg
    17821 gcaaaagagc ttggtgttca tgaagattta ataaacaaag ctccttcagc aggtctttgg
    17881 gaaggtcaaa cagatgaaga tgaaatggga actacttaca atatgataga tgctgtatta
    17941 gaaggtagat tggatgaagt tcctaaaaga gaccaagaga taattgaaag acttcataga
    18001 ttaagtgaac acaagagaaa aacgcctgca caacctccta agttctaatt tactatttat
    18061 aagatttgtg ataaaattta caagtgatga aaattagaat aattaattgt acgtattaag
    18121 gagtggaaat atgggacgta taggtaatat aattaataga aaaggtaaac aagatgctca
    18181 aagagcaaag atatttacta aacatgctag agcaatagca gttgcagcta aggaaggtgg
    18241 agcagaccca gagtacaatg cagcgttaaa aacagctata gaaaaagcta aggcagataa
    18301 tatgccaaat gataatatag atagagctat cgctaaaggt gctggagcag gtgctggaga
    18361 agactatgaa actatagttt atgaaggata tggacctggt ggagttgctg ttatagtaga
    18421 aactttaact gacaacaaaa acagaactgc tggtaacgta agatactatt ttgacaaaaa
    18481 cggaggaaac ttaggaacta gtggatgtgt gtctttcatg tttgataaaa aaggacaaat
    18541 attagttggt ttaggagatg gagtttcaga agaagaattg atggatgttg cattagaagc
    18601 aggagctgaa gatttcataa cagaagaaga tggttatgaa ataataacta caccagaaga
    18661 tttttctagt gttcgtgatg agttaaaagc taaaggatat gagtttatat ctgctgatgt
    18721 gaagatgata cctcaaacta caactgtatt gactgaagaa tctcatttaa aaatgatgaa
    18781 taaattagtt gatatgcttg aagaagatga tgatgttcaa gatatatatc ataactggga
    18841 agtagaataa atagtataag catattgaaa tttataagta ttgaatcaat tttaacatgt
    18901 gataaatttt atagaataag aaacactaaa atagacatac gcacctctaa cctatatttg
    18961 cataataata tgtaagcata ggtaaggggt gtttttatat ttataggtta agaaatttaa
    19021 agtaaatttt gagctggact cgattgtcta taaataattg atgggtatat aacttgttgc
    19081 tgaataccat ttatacgccc agttactgta tcaataatca tcctaagact acgttctgct
    19141 gtctcttcca tttttaaatc tataactgtc aaaggaggaa tacttcttgc cattagtcgt
    19201 gaaggtccat tacttgctgc aaatatttga atatcctctc caattacaat tcccgattca
    19261 tgaaatgcat tcatcatacc aagagctacc acatcatgat tacaaaaaat tacttttttt
    19321 agtgaatttt ttgcaagcat tttttgagca atatcatatc cactatctat tttattagaa
    19381 caataatata tagaatctga tagatgtatt ccatgttctt tacatgtttt taaaaaagat
    19441 ttacccctta aagtcataca tgtaaaggaa gttgggttga atacaacagc aatatcattt
    19501 ttaccatttt gaatagcatg taaagctgct atacgaccag tttcctcatt atcaatagat
    19561 acacttgaaa aaccaggtat ttcacgattt acaacaacta caggaatttt aggtttattt
    19621 tttactagat attctatatc atcagcattg atacctgtta aaacaattcc atcataataa
    19681 ttatttgcaa aaaagtattc atcatcacat agatgaccaa ctttgtaagg tcgtatgatg
    19741 atatttatgt ttaaatgttc taaagaaatt atgtggttta ttgcttgaag tatattggtt
    19801 gatgccaact caaatccctc atcctgccaa taaaaagcta ttttaatatt attatgttta
    19861 ataggagtag agcgtttata tccaagctta tcagctgaat ttaatataag ctcttgtgta
    19921 tgtagcggaa tcctgcgttc atttccttta ccacttaaga cgtacgagac agttgctgaa
    19981 gaaagaccac agtcttttgc aatatctttg attgttgcca tgataaaatc ctccttaaat
    20041 agttgcttaa atttgcttat accaataatc ttaacatata ttcaactaaa gaacaatatt
    20101 ctacaaggtg catattttac aggattagaa taaataaatt aaaaaaaact tcttacttat
    20161 gttgacataa tttagtttat aggattataa tatgcttaaa taagcttatg attaacttga
    20221 attaagcaaa gttaagcagg aacatgcaat gctagcttgc aatgtgactg caaaggggga
    20281 aaaggtatga gtaaggtaaa tatatttgaa gttggtccta gagatggatt tcaaaacctg
    20341 aaagagtttt tagaaactga taaaaagctt aagattattg atggtcttat tgcttctggg
    20401 attaaacaac ttgaaattag ttcatttgta agcccaaaag caatacctca gatgaaagat
    20461 gcaaaagaag tagcaactta ttgtgtagaa aaatatcctg atgtgaaact ttatgcatta
    20521 gttccaaatt tatatggtgc taaagttgca tgggagtgtg gtataagaga tatatcctat
    20581 gttatatctt tgagtgaaac tcataataaa aaaaatataa atcgtaccca tgaagagtct
    20641 attgatgagc ttaaaaaaat aatgaatact tatccagaca tgaatatatg tattggaatg
    20701 gctactgtat ttggatgtcc ttttgaaggt ttaccaaaaa ttgaagatgc cataagattt
    20761 gctaaacgcc tttgggaaaa cggtatcagg gagataaatc tagcagatac aatagggatt
    20821 gctgacccta agcaagtaag agaaactatt agtgcattag ttgaagcttt accagaatgc
    20881 agatggcaag tacatattca tgatactaga aatatgggaa tggttaatac tcttgtggct
    20941 attgaatctg gaattactac tgttcagtct acacttggag gattaggagg atgcccattt
    21001 gctcctggag catctggcaa tactgcaact gaagatttag tatatatgct tgaacgtatg
    21061 gggtatgata caggagttga ttttgttaaa cttcttgaat tatcaaaata tcagaagagt
    21121 tttgtaaatg gagtatatag tggtcatcat gtaaatataa agtctgatag ttgtaattgt
    21181 tagttctatt gattaatcga ttaaaaaaat atgctgattg gtaaaataga tttattgata
    21241 aaaaatataa attataaaat acattatcta ataaaacaaa taaaaaaatt gagggggatt
    21301 agtatggata ttatagcttt acttttattt ttaggagtta ttattattgc atttgtacgt
    21361 aagaataatg caggtatttt ggctctagct attggtgtca tagctgttag aatttttgga
    21421 atggatgata aagaacttta tggagctata agcaactcaa tgtttactac acttgttggt
    21481 ataacactac tctttgcaat agtgaactca acaggagcat taaagcttgt tgcagaaaaa
    21541 attgtagctg cttctggtaa acgtgtttgg cttataccta tagcagtttt tatagcagga
    21601 tttgttgttg ctggtgttgg acctggggca attccagcac ttgcaattat cccagcaatg
    21661 gctgttcctg tagccataac agttggttat aatcctgtaa tgcttgcact tataggtgaa
    21721 tgtggattaa tggcaggtcg tatgactcct attactccag aaggtcaaat tataacaacc
    21781 gttgcagaag gggttggaat tgaaaatgtt atgcctacta tcttagcctg tcaaactctt
    21841 tctacaattg tattttcaat agtattgttt ataattttca aaggatataa acttaaaaaa
    21901 cctataaatg ttatagatag taagcaaata gaaaaattca atataaagca gataatttct
    21961 ctaataagca tagtagttat gattgctttt atcataattt ttgatatgaa tgttgggctt
    22021 gcagcttttg cagtttcagc tgtacttatt tttttcaata ttgctgatga aggagaatgt
    22081 atcaaatcaa ttccatggag cacgatagta atggttcttg gagtaggtgc aatgatgaca
    22141 atcgtagatg cagcaggagg tattgacttg atgagtaatg cattatcatc actgatgaat
    22201 agtaaaacag ctactccaat tatgagtatt tcagcaggac ttatgtctat ggttagttct
    22261 gctttagcag tagtttatcc aactatgatg cctatgtgtg ttgacatagc taaggcagtt
    22321 ggaggagtag acccactagc acttattgct gcagttggtg ttggaggttc tcttgctggt
    22381 gtaagcccac tttctactgg tggggcattg attttagcag ctatgggttc aagtaagaaa
    22441 gactttaata aggaagaaca aaataaggtt tttgtacagc ttttcattat gagtgcagtt
    22501 ggcttattgg ttattgcagt agtatcaata ttatcattta atgcaataac acatttaatg
    22561 aactaattag aataactgaa aataaatcat taaatttaac ttttaatatt aatcaatata
    22621 atataatttg gaggggaagt tatgaaaaaa ggagcattaa gcaatataaa ggtacttgac
    22681 cttacaagag ttcttgcagg tccatattgt acaatgatgt tggcggattt tggagctgat
    22741 gttataaaaa tagagatgcc aagaaaaggt gatgatacaa gaggttttgg accttttgta
    22801 aatggagaga gtttatacta tgcaaatata aatagaaata aaaaaggtat cactctaaac
    22861 cttaaatcag aagaaggaaa aaatatattt ttagaaatgg taaaacatgc tgatgttgta
    22921 gtagaaaatt acagaccagg tgtaatggaa aagttaggtc tgggttatga tgagttggct
    22981 aaagttaaca atcaaataat ttatggagct gtatcaggat ttggatgtta tggaccatat
    23041 tcagaacgtc caggttatga tattattgca caggcaatgg gtggtcttat gagtataaca
    23101 gggcaagaag gaggagaacc aacgagagtt ggtaatgcta tgggtgatgt actaggtgga
    23161 atgaatttaa ctattggtat actcacagca ctcaacgcta gaaaaataat aggaaaagga
    23221 caaagagtag atgtttccct cgttgattct gttgtatcgt ctctcgaaac tggtatacag
    23281 agatactttg attcaggaca acctgaactt atgggtaata gatatgcttc agcctatcct
    23341 tatgactctt ttaaggcaag tgatggaagt tttgtaatag gctgtggtaa tgacaaactt
    23401 tttgaaaagt tatgtaaaaa agttcttttt agagaagacc ttttaataga tgagagattt
    23461 aaaacaaatc ttaatcgttg tgaacattac aaggctctta aaccagaaat agaaaaatgg
    23521 tctgttaagc atacaataaa agaagcagtt gacttaatat cagaggctgg agttcctgct
    23581 gctcctatct ataatcttga ttgtgttaca acagatgaac atatagcaaa agcaagagaa
    23641 atgattgtag atattagtca tcctattata ggaaatatga aagtcaatgg aaatccagtt
    23701 aagttgatga caacaaaagc taaaattgat atgccagcac catctctagg ggaaagtaac
    23761 gaggagattt ataatggttg gcttggtttt gatagtgata aaataaaaga tttaaaagaa
    23821 aatggagtta tttgattaaa aaatagctaa acacacttgt gactttagtc atgggaggtt
    23881 cagttagaat aatatcaaaa aatattaaat caatactgta aataaaatat agaattgtca
    23941 gaagcattta agatgggaaa agctacttag acattgagtc tttgtggctt ttcctatttt
    24001 cattaagaat tttatattat cagaatagat atgtactaaa ctaaagtatt aacaatttaa
    24061 attaatagaa aataaactac ctaattatgt tgaataaaga ctactttagt gaaaataaac
    24121 ttatagagat gctatttaaa ttattttaat ggaaatatca aaaatgagac tgcattctca
    24181 aaaatgagac tgcgtttttt aagtattttc tcgaaaatga gacaaaaata ttgttttatg
    24241 ggttttattg aagaaaaatt cttctgtgaa ttacaattta atcaatttta aagttgaaaa
    24301 ttattaaaaa tttagtgaaa tttatgtata tttcgtttat tttattaaaa ataacaattt
    24361 tttactaaaa ataaagaact tcaatggtga ttggcatagt acttgctatt ttatattaac
    24421 aagaaatgta gaaaaaataa caatgtataa tggggggaat atgatggaat ctgtaatgga
    24481 aaatttgaac aatcttaagg tggaggttaa ggacaaagtc tgtgttatta ctataaatag
    24541 acctaaagct ttaaatgctc ttaatagtga cactcttagg gaactatcac aagttattga
    24601 tgtagtatca gaaaatgagg caatattagg agttattatt actggagaag gtaaagtttt
    24661 tgtagccggt gctgatatac gccagatgca aaactataaa agtgaggaag gcagaaaata
    24721 tgcaggttat gctcagggaa tttttgataa aattgaagct cttgagaaaa cagtaatagc
    24781 agcagttaat ggatatgcat taggtggtgg atgtgaactt gctatgagtt gtgatattag
    24841 aattgcatca gaaaaggcta tatttggaca acctgaagtt aatttaggag ttattccatg
    24901 ttttggtgga actcaaagat tatcaagact tgttggaact ggaatagcta aagagttaat
    24961 ttttacgggg agacaagtaa atgcagaaga agctaaatct attggactta ttaataaagt
    25021 ggttccaagt gatttacttt tagaagagtc tatgaagatg atgaaccaaa tagttgaaaa
    25081 agcgccaata gcaataagat atgcaaaagt tgttattaac aaagggatag atatggattt
    25141 aaagaatgct cttgaactag agaaagatgt tgcaggttta acttttgcta caagagataa
    25201 gcaagaagga atgaatgcat ttatagaaaa gagaaagcca gtttttgaaa ataaatagct
    25261 attataacta aaattggaac aatgtagaat atattcaaaa tctaaatttc gtgaaaaaat
    25321 acatatagtt taattttaaa aggagttaat ttatgaatac gggagtaatt ataagtttag
    25381 tagggattat catagctcta tctcttctag ttgtaatggt tatgaaggga ataaatattt
    25441 ttattatagc aattgtatgt tcatcattag ttgctataac aggaagccta aatgtgtatg
    25501 atgcacttaa agtggattat atgacagggt ttgttggatt tcttcaggca aatttcttta
    25561 ttttcctaac agggacatta atgggaaaaa taatggagat tactggtgga gcaaaatcta
    25621 ttgctaagat gattgtaaga tggattggga aggataaagc actactttct attccaatag
    25681 cttgtggaat attagcctat ggtggagtaa gtgttttcgt tgtaagtttt gcagtgtttc
    25741 cgatagcttt agaaattttt aaagaagctg atttgccacg aagatttata ccagcagctt
    25801 tgacttttgg atgctcaact tttgctatgg ttgcaccagg ggcaccacaa attcagaata
    25861 ttgttccagc atcaacatta gggacagata ttatggctgg agcagtaaat ggttttatat
    25921 catgtgcagt aatgtttata gttggaagta caattttgta caaaatggtt tcaaaagaaa
    25981 aagccaatgg tggtcatttt atagcacatg aatcagatat atttgatgaa gcagaagcaa
    26041 ctaattctaa gagagaaaat ggtcccaatg gtgtaattgc cttaattcca ttgatagtgt
    26101 cgattttaat aattaatgta aaagttaatg gaaaagcgat agtaccaatt gaggtgggag
    26161 tatttattgg agctttatta gtatatttat tgttaaataa atatcaagat aatactaaaa
    26221 tagttggaca tgttggtgat gcatgtaaaa ctacagtagt agcaatatgt aatacttgtg
    26281 ctgtagttgc atttggttca gtagtaaaat cagcagttgg ttttgacttc atagttaatg
    26341 ctatgactaa tattcctggt cctcctattg caggggctgc agttggaact acagttattg
    26401 caggtatttg tgggtcagct tcaggtggac taggaattgc agctccactt cttggaccag
    26461 tatttttagc caagggtgtt tccgctgcag cattaactag gactatggca atttcatcag
    26521 cagcattaga ttcacttcca cacaatggat atattgttac tgtaacaaat ggtctttgta
    26581 aagaaacaca taaggatgcc tatatgccaa tattctgggt aactgtttta accccattta
    26641 ttggaaccat agtagcagtc ctactgttta caatgttccc gggattgcca taggagaaaa
    26701 gataaaaggt ataaattata ttttgatatt ataaaatcaa ttttcaaaat atatacaata
    26761 aatttcatgt tttaatactg taaatatgta tttaaaataa caaattgacc atataaggag
    26821 gaattgaaag tgttaaataa accattagag ggagttaagg ttattgacct aacgtatttt
    26881 gtagcaggtc cagggacttc taaaatatta gctgactggg gtgctgacgt tattaaggta
    26941 gagccaagct ttggagaccc aggaagaaag acaggggcaa caatgacaat gccaatagat
    27001 gattataata atccttttta cagcacatat aattcaaata aaagaggttt gtctataaat
    27061 cttaagagtg aaactggaat tgaaattatg gataagcttt taagcgaagc aaacgtattt
    27121 gtttcaagtt atagaacagg agcattaaaa agactaggtc ttgattatga atcattaagt
    27181 aaaaagcatc cacaccttat ctggggtcag ataaatggtt ttggcgattt tggacctgct
    27241 aaggataatg caggatttga tacagttgcc ttttgggcaa gaagtggggc tatgcttgat
    27301 atagcagaaa aagatacaag tccaataaat ccacctatag cctttggaga tgcaacaact
    27361 tcttgtagtt tatctggagg aatatgtgca gctttgtacc agcaagctaa gactggaaaa
    27421 ggacaaaaag taatggcatc tttatttggg caagcaatat ggaatgcatc atctttatta
    27481 gcatctacac agtttagtga tgagtatcca aaaactagaa aaaatgctat ttcaccagta
    27541 ataaattctt atcgttgtaa ggatggaaag tggatattct taagtatttt agagcatgaa
    27601 agatattttg aggcactttg tagattattt ggaagagaag acttaataaa agaagagaaa
    27661 tttgcatctt cagtagaaag taagaaaaat gctacagaac ttattaatat tttagatggt
    27721 gaatttgcaa agtttactca aaatgagatg gttgaaaaac ttactgctgc tgatatagct
    27781 catgaaaaaa ttcaacatgt taatgatgta gtgactgatg aacaggctat tgctaataac
    27841 tatatatatg aacatacaag taaagatgga aataagacaa tgcttgcatc tactccagtt
    27901 aaatttggaa atattgaagt taacatgact tgtgatgctc cacttatagg agaacattca
    27961 gatgagatat tgagagaact tggatacagt gattctgata tagagactct aattgaatct
    28021 ggaattgtaa caatgaataa agaagcatgt gaaatatgtt aataaacatg tgaagtatgt
    28081 taataaatag gagggaattt tatggatttt agattaacag aagcacaatt aatgcttcaa
    28141 agagtagcaa aagaatttgc agagaatgaa atagcaccaa tagctgctga aacagataaa
    28201 acaggtattt ttccaaggga gctttttagc aaaatggcta aaataggatt taatggaatt
    28261 ggaacacctg tagagtatag tggttctggt ggggcagata tagaaaaggt aattgttgtt
    28321 actgagattg ccaaaaaatg tgcagcatct gcagcaatat tatcaataca cacaatatat
    28381 gctcaagcta ttttgaaatt tggtactgag gaacaaaaga aaaaatacct tccaatgatg
    28441 gctgaaggtg ggtgtgtagg tgcttttgcc ttaaccgaac ctaatgctgg ttcagatgct
    28501 gcgagagctg caacaactgc aattattgat gaagaaactg atgaatatgt tttaaatgga
    28561 actaagtgtt ttatttctgg tggaggtcaa gcagaatcat taataatatt tgcattgaca
    28621 gacccatcaa agggaataaa gggaatgtct gctatcattg tcgacaaagg tactccaggt
    28681 ttttcaatag gaaaaattga agaaaaaatg ggtattcatg gttcagaaac tgcagagtta
    28741 atatttgata actgtagagt tccaaagtct aacttattag gaaaagaagg taaaggattt
    28801 aatatagcta tgacttgttt agatggagca agaattggag ttggagctca agctgtagga
    28861 attgctgaag gggctttaga agaaagtatc aaatattcta aagaaagagt ccagtttggc
    28921 aagccaattt cagctttgca aggtattcaa tggtatatag cagatatggc gacaatggtg
    28981 gaatcagcaa aattattagt atattatgct gcagatttga aagctagagg agaaaaacac
    29041 actaaagaag ctgctatggc aaaatacaat gcgtcacgta ctgcaagaga ggttacaaat
    29101 ttagcccttc aaatccatgg tggatatgga tatatgaagg attatccatt agagagaatg
    29161 tacagagatg ccaaaataac agaaatttac gaaggtactt cagaaataca caaagttgtt
    29221 atttcaagag cagtactagg ataggaggaa aagaatgaat atagttgttt gcttaaagca
    29281 agttccagat actaatgaag ttaaaataaa taaagagaca ggtactctaa taagagatgg
    29341 tgtaccaagt ataatcaatc cagatgatag aaatgcttta gaagaagctc taaaaatgaa
    29401 agatgaacta ggagcagtga ttaaggttat aagcatggga cctcctcaag ctaaaagtgc
    29461 tttaaaagaa gctttggcaa tgggagctga tgaagcttat ttaatctcag atagagcttt
    29521 tggtggttca gatacttggg ctacatctac aattattgct gctgctatag agaaggttgg
    29581 aaagtatgat gtaatattct gcggaagaca agctatagat ggagatacag ctcaggttgg
    29641 accagaggtt gcagaatttt tagggatacc acaagttact tatgcgaaag aagtaaaggt
    29701 tcaagatgac aaattactag taactcgtta tacagagaca ggggattatt taatagaagc
    29761 aaagatgcca gttttattaa ctgctataaa agagttaaac aatccaagat atccaagtgt
    29821 caagggaatc ttagaagctt ataataatgg agatgcaaag attactgtat taacacttgc
    29881 agatttagat gttgatacaa ctcaaattgg attaaagggt tcacctacaa acgtttataa
    29941 gtcttttgta ccagttaaag acaagcataa tgaaataata gaaggtataa ataaaaaaga
    30001 aaaagctgag aaactaatag aaatactatt cgatttgaaa ctagtatagg agtgttaaga
    30061 tgatgagagc aaaagttaat caaggtataa atttaaatga ttataatggt gtttgggtaa
    30121 taggagagca aagggaaggt aaaattaatc ctgttacgat agaacttatt ggtgaaggaa
    30181 gaaagcttgc tgaccaatta ggaaaagagc ttgcagttgt gatagctgga tatgaagtag
    30241 agaaagaagt gaaggaatta cttcactata gtgttgataa agtatattac atcaatgacc
    30301 ctcttcttaa agatttcact actgatggat atgcaatttc aattgcaaat ttaatcgaaa
    30361 gaaaaaagcc agaggttgta ttggttggtg ctacttctat aggaagagat atagcgccta
    30421 gaattgcagg taaggttgga acaggtctaa ctgctgactg tacaaaactt gaaatagatt
    30481 caacagataa taagttatta caaacaagac cagcatttgg tggaaattta atggccacta
    30541 ttgtttgtcc aaaaaataga ccacagatgt caacagttcg tccaggagtt atggcaaaag
    30601 ctgtaagaaa cgaatcagag actggaattt tagaagttgt tactccagaa cttactgaaa
    30661 aaatgattag aactagatta gtagagatac ttccacaaga aaagaaatct gtaaatctaa
    30721 ctgatgctag aataatagta tcagggggaa gaggattaaa aagagcagaa ggatttgagc
    30781 ttataaaaga attggctgat aaattggggg cagagattgg tgcttcaaga gcagcagtag
    30841 attcagggtg gatagaacat tctcatcaag tgggacaaac aggaactact gttagaccag
    30901 aactatacat tgcatgtgga atatctggag caatacaaca tttagcaggt atgagtgatt
    30961 ctaaatatat agttgctata aataaagatg caaaagcccc tatttttagt atatgtgact
    31021 atggaatagt aggagattta tatgaaatta tacctgaaat gatagaatca ttaaataggt
    31081 aacttttaga aaacattgaa tatgtagaat gataagtctt ataatattgt attgtaagac
    31141 ttattatttt tgctgaaggt tatagtgggg agatgaaaca atgaaagagc agtggtataa
    31201 ggatatattt gcaagagtat tatctatgac tgatgatggt tttattgttg taaatacaag
    31261 tggagtaata atagatatta atgataaata ttgtgacttt ttaggaaaag aaagaaaaga
    31321 tataatagga caaaatatac aaagtattat accaaataca aaaatgctag atgttatgaa
    31381 aaataagtat tgtgaagaag gagctataca tcattattca ggtggaaata caaaagaaaa
    31441 aagtgtaata gtaagtaggt cttatgtgga aaatgacaat ggagaagtag ttgcaggagt
    31501 agctcaagtt aaatttagat tgcaatcatt tgatgtagct aaaaagttaa tgtcagaata
    31561 tatggagctc caatattata aagagcagtt taaggataac tgtggttttg acaagctcat
    31621 aggtgaaaat agagacttca tagaacttaa gaaaacaggt gtgaaagctt ctaaaacaaa
    31681 ctttccagta cttttaacgg gtgaaacagg cacaggtaaa gaagtttttg ctagagcaat
    31741 tcacaataat agcagtaggt cagataaacc tatggttagt ataaattgcg ctgcaatacc
    31801 agaagaatta ttagagtcag agctatttgg ctatgacgaa ggagctttta caggagcaaa
    31861 aaagggtggt aaaaaaggta aatttttagt tgcgaataat ggtacaatct ttttagacga
    31921 gataggtgac atgcctttga ccatgcaagc aaaactttta agagttcttc aggaaagtga
    31981 gatagaacca gtgggaggtc ttaaaactat aaagatagat gtaagagtaa tctctgccac
    32041 aagaaagaat ttaagtaaaa tggttgaaga gggattattt agagaagacc tatactatag
    32101 attaaatgta ataaacattc atatgatgga attgaaggac aggcaagatg atattttatt
    32161 gctagcaaat tatatattaa acaaattaaa tgttgaatac aaggaattga aggttttaag
    32221 tgataaggtt aaaaattgtt ttattaacta tacttggccg ggaaatataa gagaactaca
    32281 aaatgtaata aaaagtgcct atgctgtaag tgatgatatg gtcattatga tgtgtgacct
    32341 tccatctaag atggataata tcagcagagt agctcaatgt aatgtggata gcaattgttc
    32401 aattcatgaa atggttgaga attatgaaaa gagcttaatt attgatgtgt taaggaagta
    32461 taattggaaa tgttctaagg cagcagaggt tatgggaata cacaagagtc ttttatataa
    32521 gaaaataaaa aagtatgaaa ttgaattaaa taattaagat aacttgaatt aaggcaatgc
    32581 taaaaaagcg cccttattca aagcgaactg tataaactgt tttatgcagt taaaacgtgt
    32641 aaaagtagat gtgaagtact aatgtcaatc attagtactt ttttattata tttataatta
    32701 ctatctatat ttcagtatct tttgatatct aagattatta tgtttaattt agtatataac
    32761 aaaaccctct tagttatgga cagtttttta tattttaact gtctactaag aggggatagt
    32821 ttagtttaaa tttagagtaa attttatcta gcatttataa aaaatatttt gaatagcttt
    32881 tttgttattt agtcataatt ttctacttct ttatcaagtt ctttgccaca agaatcacac
    32941 ttatatatag cattgtatcc gtaatcatat tcatcaacta ttttcttttg tctaaataca
    33001 ccaccacact cacataggaa accaattttt ataagtgtaa cgataaattg tgattttata
    33061 aattttatat cattgcattt atcgttagtg taaaatgtat aaatataaga gttatcgcac
    33121 tcatctctta attcaaatct ttcaatatat gtgttatttt ttaattcctc aaattcttct
    33181 ttagttaaat atgatttttg ttgcaattca tgtaaacgct taatttggtc cattatttat
    33241 atcctcccaa taattttagt attttaagct tagataagga atctaagttt tattaaattt
    33301 atcccaagga attatttaaa tcatatttag ttttattaat tacaacatat ataaattaca
    33361 cgaaaataaa actaattgta aaaataatat cctgtgaaaa cctaaactac taacccctta
    33421 ctaacccgaa aactaaaaaa agaaaatata tccaaatata agtgatatat aaataaagat
    33481 gtttcatata aatttaactt taaatattct gacaataata tatataaata tattatagtt
    33541 aaaatatacc acatattttg ggtattgtat actggaatta gagtaattaa tttatttttt
    33601 aacatacaaa tgattttatg aaaaaaaaca agactcaaaa atttttatat tctttgatag
    33661 aataaaattg agagtaaatt tattgaattt tataaaattt ataaggggtg agtctaagat
    33721 atgcatgaag ataaaaatga tatatttgaa tcaatagata ctgaggattt tgataataaa
    33781 acagaagaag aatgtttaga gctagtaaga aaaaatggat tgaatttaat atacataaaa
    33841 aatcaaacag aagagatatg tctagaggct gttagacaaa attgtaatgc aataaaatgt
    33901 gttcagaatc agacagaaaa aatatgtata gaagcagtta aacaagattg gagaatgtta
    33961 gaatttgtgg aagagccaac agaagaaatt tgcatgttgg ctattaacca agatggaaca
    34021 gcattaaaat atatagaaaa tcagacagag gagttatgct taagagccgt tgaaaaaaat
    34081 ggtgcagctt tggagtatgt aaaagaacaa acagaagaaa tatgtataga agcagttaga
    34141 aacagcgaat ttggattggc aagagtgaaa aatcaaacag aaaaaatatg tatggaagca
    34201 gttaagcatt gtagctataa tctaaaatgg gtagaaaatc aaacagaaga aatatgtatg
    34261 gaagcagtga gacaaaatgg gcttgatttg aaatttgtaa aaaatcaaac agaaacaatc
    34321 tgtttaagag cagtaagaca aaatggaatg gcattagaat ttgtaaagga acagactgtt
    34381 ggtatatgct taaaggcagt aagacaatat ggaatggcat taaaatttgt aaaggaacaa
    34441 acagaagaaa tttgtacaga agctataaaa caagataaaa gagcacttag ttttgtaaaa
    34501 ggagataagg aaaaatataa ggctctatat gataataatg aaccatttgc aaagagatat
    34561 gttagaaatg taatagaaaa agaaaacaga gcattgatta aaaaaggaga agaaaatgca
    34621 aaaatcaaaa ttgcaggaaa actttatgat agaggcatgt cttttgaaga tatatcagat
    34681 atagttgaga ttgaaataag caagttaaaa gtgagtttag gtgtttttta aagtaataaa
    34741 tgtaggtgtt ttttatggga aatagttaat aaaaaaataa tcgactagct ctttgtagga
    34801 cagcaaaatt agtgatataa tttgatgtat aataaattat ttttattaga taagggggaa
    34861 ttaaagttga agattacagt attataccaa acaagaagtg gaaacacaga aagggtcgct
    34921 aagctgatag aagagggcgc taaaaaggtg gatggagtag atgtaaagct tatgagactt
    34981 gataatattg atttagatta tttaaatgaa agtaaagcag taatatttgg aacacctact
    35041 tatttagcaa atacaacttg ggaagtaaaa aaatggtttg atgaagattc aaaaaaagtt
    35101 aatttagctg gaaaattagg agctgttttt gctacatgtg attatatatg tggaggtcca
    35161 gatgttgcga tacttactat agtaggacat ttgatggtta aagggatgct tgtgtattct
    35221 ggaggtggtt ctttaggaaa gccatttata catttaggac atgtacattc aattgaaggt
    35281 ccagagttac aagatgagaa ggctataata tttggtgaaa gaattgctac taaggcaaaa
    35341 gaactatttg cttaatttct atatgcatag taatgattag agtaaattta gggttttaat
    35401 ggagatttga agtaaaagtt ataaaataaa aaattttcca taaaattact caagatgaat
    35461 tataatataa ttataaataa attatattat taaaatattt aaaatcaaac gtcaatagcg
    35521 agggaagttt atggtatggc atatagaata tgagattttt tctgcaatta tagttatttt
    35581 tttaatggta tactttttta gaagtaaatt tatacctact ttacaaaaca aaatatattg
    35641 tgcactttta atattctcat ttttatttat aatttcaaat attttaggtt cattttgtct
    35701 aaataatata gacaaaattc caatttttat tactttttta ttgaatcaga tatacttatt
    35761 gttattgcca ataccagcag cacttgtatc attttatgtt atggctatta tttatcaaga
    35821 tataaggtat atgaaaaaaa gattattatt tttatctata ccacttataa tttgtataat
    35881 cttatctatt acaaatcctt ttacgcatat tctatttgga ttgtcaaaag aaacaggtta
    35941 tatacgaggt tgtggatatg ttgcaacttt tgcatctttt ttcttttatg caatatattc
    36001 agcattttta tcttttaaat atagaaaagc aatgcatgaa tcaaaaatat gggctattag
    36061 aggttttctt atagtttcaa ttgtggcaat tatcttacag gcaatttttt tacagtatct
    36121 tcttacagga actgcatgtg catgtagttt acttttgaca tatctttcta tgcagaatcg
    36181 aggtcttatt attgatgacc ttactggtgt actcaataga caatctttta ttcaagagct
    36241 agatttgaat atcaatactg aacaatcagg atttattatt acaattgcgc ttgatgattt
    36301 taagtttatg aatgaaactt ttggaacaaa aaatggagat attgcactaa aagaagttgg
    36361 aaaatatttg attcagatac atgatgtaaa ccatgtatat agaatagggg gagatatatt
    36421 ttctattgtt gtcaatagtc aattaggtat agaaccagat gatattatca gaaagataga
    36481 agaaagattt aaaaagcctt ggctagtaga agagataagt tttaatttag caactagtat
    36541 tgctgttgtt tattacccag aaaatgcaga aacgacagaa gatgtaattg ttgctataga
    36601 tttctctata catgaagcaa aacgctctga gtcaaggcga gttgtttatg cagatgcgtc
    36661 tataagtgaa aagataaaga agaaacatgt tattaaaaat tgtttaaaag aagcattaaa
    36721 aaatgatggg tttgaagtgt actaccaacc tatattttct aatagcaggg gcaggtttac
    36781 ttcagcagag gcattactta ggctagaaca caaagagtta ggttttatac ctcctgatga
    36841 atttattcca attgcagaac aaacaggatt gattaactca attggattag tagtctttga
    36901 gaaggtatgt agatttatag ctagtgatga gtttgaagat ttaaaattgg ataatatagc
    36961 tgtgaatctt tctgtagtac aatgtatgca aaaaaactta gctgatgatt tactttatct
    37021 tatgaataaa tataatgtat caccaactaa atttaaactt gaaattactg agactgtggc
    37081 tatgggctct tttaatataa ttaaagaaac aatggaaaga ttaattgatt taggtgttaa
    37141 gtttgcactt gatgattttg gaattgctta ttctggagtt actaatatgc tttctttacc
    37201 attttcagta attaagttag ataagagttt gatttggtct atgaatgaag atagtaggca
    37261 taaacttaca gttgagacga ttatagcact tataaataaa ttgaatatga aggctgtagc
    37321 agaaggtgtt gaaaccatta agtgtgctga agatttaata gctatggagt gtgaatattt
    37381 gcaagggtat tatttttcta aaccagttcc agaagatgtt tttaaaaatt tgttgaaaga
    37441 aaatactttt acaagttaac accctttatt tttttaatta caactttgtt cattgacact
    37501 gccagcgata tattataaaa ttaacacaat aataaatatt aaatttatta tacatattat
    37561 taatattaat taatggagta aagatttatt atatatttta tttaattatc ctaaagtgag
    37621 aaaggaagat gtgacatgaa taaaaagaaa atattagtgc caattgatgg gacagaaaga
    37681 agtatgcatt ctttggaatt tataaaagga atatttaaaa aagatgaagt tgaagtagaa
    37741 ataatgaatg taaaagaact agtatttatt gatggcatat ccctggctga agagataaaa
    37801 aattctgaaa atttaggaag aagaatcttg gataaagcag cagagataat gggagagtat
    37861 gatgttaaag tacattttac ttttggatat ccaggagatg agataattag aaaagcaaaa
    37921 gaagataatg tagattttat agttatgaca aaatctacta aaaaaggtct tacaagaatg
    37981 ataggttcag taacagcaag tgtagttaaa caagcaaaat gtatagtgat gattgtaccg
    38041 gagtagaatt agaaaggtat ttgaaatatg aataaaaaga tagtagcagt ttgtgaaagt
    38101 gaaagaagtt taaattctct taagacagct gcaaatgatt taggatttga tttagtttat
    38161 gaaattcaag aaaatgataa tataaaaaat gaactttcta tttatgatat agaagatgca
    38221 tccatagtgt tatttgttac aagtgattct atagaaagca tagagaagat tgatagattt
    38281 atagatagag aattttatga agttgatcca aagtatataa tagaggatgc aaaatctata
    38341 gtaagtgaaa ttttaataga cttaaattaa tcgtgggtgt cgcactaaga atagagcgac
    38401 gcctttttat atattttaca caagaaataa agaggttgta tcaaaatata gataagagtc
    38461 tttttcattg ttgaaatgat gaaaatatat ttgatttcat acaaaaaaga gtacctcata
    38521 actaaaaagt tcactttgag acactccatt tttatatgtt agttgcattt aaggtaaaat
    38581 agaagtttaa tcatgttgaa ataactgaat tacatcttct aaatgattgt acattgatgt
    38641 ataacctaag taagagtctt tactatcaaa tgcaatcatt atcttgttat ggtggctaat
    38701 catatccttg agagaatttc tatatttaaa accaaagtat tggttagaag aaactgtttt
    38761 aggtattaac tcataatatt ttataaaaat tggattatga gaagctaaaa ttatctcttt
    38821 gtgaaattgc aagtcagcat cataacattc ttctatcaaa ccatgagaaa ccttatccaa
    38881 ttcctctatt gactttaggt aagtatattt tattttttga agttctttag atgtagcatt
    38941 taaaattgct agtttagtag cttcgctttc gataagttgt cttgctctta aagattctac
    39001 ataaaattct tgtatgttat gatttttata gatttcacca tcatcaaata ggtcaacttt
    39061 tttaaatcct tcaactatag gacttgaata aggcacagct ctaagtattc cttccttaca
    39121 gagtatattt atggcatttc ttacaggtat tctacttatg ttaaataggt cagcaatctt
    39181 tctctcactt ggtaatttac tattaatttt aaaagttcca tttagaatgt ttaatttaat
    39241 ataatcaata attttttcag tggcatttaa gcttttcata aaaactccta aacatataat
    39301 ttttttatga ttgtattata tcatagttaa gagttaaatc tactttttta taatatgtat
    39361 aagacaggat agaggccaaa atagctataa tagccaagat aaagtatcca tattgtattg
    39421 aagtatattc cgatatttta ccaactatca ttggaaatac agacattcct atagcataaa
    39481 ttgcttgaaa aaaacccatt gcagtagatt tctgatgttt tggtacattt ttcatagact
    39541 cgctagtaag gtaagaaagg agtatacctg tagatatacc tggtagtact tgtagtacaa
    39601 atattatatt aatatttttt attgttggaa ctaaaaagca ataggctgaa actaaaataa
    39661 aaactaaagg aatccaaaat tttggtccat acttagtaca aaatttagta gaagcaaagc
    39721 cagaagaagc aacagcacac accatgtaaa atatagaaga tagacctatg agggtagaac
    39781 tagaacctaa gtttttaaga atttgagtag taaatgacat tgttgtagac atttgaatac
    39841 cctgttgaat taatgccata aaggaaaaaa atacaagtct tttatccttg cagatagata
    39901 aatatttttt aattgatatt ttttcaggta tttttttaga attatgattt tttatattta
    39961 aggctagaat cgttccaata actgaagaca atacactcat gatacataag gctgtcattc
    40021 ctaatttttc ataaaataaa gtactcataa caaatccaat caacattcct agattgttta
    40081 ccaatattat cgtacttgta gctttttgtt gatgttctgt agaaaaaaag ttggtatagt
    40141 gaaccatgta agaaatccaa gttgatgatg atattccaga taaaatgtta gctataagaa
    40201 aaccattgcc tccaggagaa aagactctta taagagatgc taaaccagca aagaaaattc
    40261 cagacagtat aaatatttta tgtttattta tggaatcagc aaatactcca acaggaagcc
    40321 ttaataaaca ttgtgatatt ccatatgcac ctattattat accaatcata tttgtagata
    40381 cattacatgc acttaaatat ggattttgga aaggtatata tacatattgt gaaaaccaga
    40441 aaaatgtaac aatagaaagt aaaagtaatt tttgattaga tatttttttc atattatcct
    40501 ccatgtggta tttttataat gcaaataata taccatttag attttttaat caagtacttt
    40561 ctctattaaa aaaaatttaa atatataatt attatttaca aaaaaattat actttatagt
    40621 tgaattaaga aaacgcttat gatactataa agttaacaaa tttgaatata taacagtaga
    40681 cgtgttactg gtaatgcagg caagatcgaa tcaattttta tagattatct atagagtact
    40741 atgggtataa tctatttatt tggttcgatt tttttgttta attggccata aaacaaaaag
    40801 cctatagttg aaaatggagg attttttatg gattataaaa atatagctca agaaattcta
    40861 ctaaatgtag gaggaaaaga gaatgtaaat gaagtgactc attgtatgac aagacttaga
    40921 tttaaagtca aaagcgcttc taaagtgaat aaggataaat tatctaaaac tgaaggagta
    40981 ataactgttg ttgagagtat gggtcaaata caagttgtaa ttggaaataa agtaaaaaaa
    41041 gtatatgatg aggtcataaa aattgtacct caatcaaata acataggtaa aaagaatgaa
    41101 agtcaagaaa agcaaggtat aattaattct atattatcag cagtagctgg tatatttaca
    41161 cctacaatac ctgctatagc aggggttggt atgattaaag gtattttatc agttcttgct
    41221 atgtattata tgaataaaaa tggagtagat ataaaagaaa cgcaaagcta tattatctta
    41281 aatgctatgg ctgattctat attctatttt atgcctatta tactaggata tactgcggct
    41341 aaagtattta atgcaaataa aataatatct atggtattag gagctacact ttgttatcca
    41401 acttttactg ctttgatggc aggtgaagaa tctgttagat ttttaggatt ggcagtaaca
    41461 aaagctacgt atacatcatc tgttattcca ataattattg ctatatgggc tctttcatac
    41521 gttgaaaaag tattagaaaa gtatatacct gaggtaatta aaataataat ggttccaact
    41581 ttatcattgg tagtaatgtt acctgcaaca ttatttttat ttggaccaat cggaatttat
    41641 ataggaaatg tcataaactt ttcttataag tatatttatg aattaagccc agcattgtgt
    41701 ggagcatttg taggtgggtt atggtgtgtg ttagttatat ttggtgctca tagagcatta
    41761 ttaccaatag gtataagtga tgttgctcaa acaggaagac aaaatttatt ggcgtttgca
    41821 ggagctgcaa acttttcaca agcaggggct gctctaggag tattctttaa aactaaaaat
    41881 caaggactaa aaacaatttc tatgtctgca actataacag ctttatttgg tataactgaa
    41941 cctgcaatat acggtgccaa tcttagatta aaaaaaccta tggtatgtgc cgtaatatgt
    42001 ggtgcatttg gaggtgcgat aatgggaatg ggtggagctt atggtaatgc atttgcaaac
    42061 caaggtgttt taactatacc tgtatatgct gaagcaggtg cattaggttt cttaagttat
    42121 ctaggtggtt gtgctatagc attttttggt tcagctattt caacttatct agttggattt
    42181 gaagatttgg aagagtcttc taataaggaa aattcatcta taaaagtaga aacgaaggat
    42241 ggtgttattg atataacttc acctgtagag ggcgaatgta tagaattgtc agaagtaaag
    42301 gatgatgtat ttgcttcaaa agctatggga gaaggtatag ctgtgttacc tacaaaagga
    42361 gtaataacag cccctactga ttgtgaagta gcttctttat ttccaacatt acatgcaata
    42421 ggattaaaat tagataatgg agcagaaatg ttaatacatg ttggaatcaa tacagtagag
    42481 ttaaatggta aatactttac aaaacatgtt aatcaaggag atttagtgaa aaaaggtgat
    42541 aaacttatat cgtttgatat agataaaata aaaaaggctg gttatgatgt aactacgcca
    42601 gttatagtaa acaatacatt tgattttggg caagtagttt cttgtaaatc tagttatgtt
    42661 tcaaccaatg ataatataat ttcattagta ttagctggta attaagttaa tttgactaga
    42721 gataagtaaa ttgatttaaa aggaaataag taaatcaaca attaaataca tctaaaagta
    42781 agtttattgc aatgaattgt taaagaaaat aattaatatg gagtaaatct attgcaatga
    42841 agtaacaaag aaaataatta atatagagta agtttattgt aatgaattgt taaagaaaag
    42901 agttaatata gagtaagttt attgtaatga attgttaaag aaaataatta atatagggta
    42961 aatctatctc aatgaaataa caaagaaaag agttaatata gagtgaactt attgcaatga
    43021 atcaacaaag aaaataatca atataaaaag gagaaatatt tatgaataca ggatttccaa
    43081 aagacttttt atggggagca tcatcttctg catttcaagt ggaaggagct tgggataaag
    43141 acaataaagg aaaaaccgta gccgactata attcttttaa aaagtctcat ttacaggcag
    43201 atactaaagt cgcatcagac ttttaccata attatgaaga agatattgaa ttaatgaaag
    43261 aattagggat gaaaacatat cgtttttcaa tttcatgggc aagaataata ccagatggtg
    43321 aaggagagat aaaccagaaa ggtttagaat tttataataa gataatagac aaactgatag
    43381 agtgtgatat tgagccattc gtaactttat accattttga ccttccattt aaattagtag
    43441 agaaatataa tggttgggaa agtagagaaa ctgtctatgc ttttgaaaga tttgctaaaa
    43501 tatgctttaa gcattttgga gatagggtaa agtattggca acctcataat gaacagaatt
    43561 taattgttag agtggaggag agaattaata tatatgatga aacagattca tggaagatag
    43621 ataaaatgcg tgctcagatg gattataatc tttgcttagc acatgcatta gcagtcaatg
    43681 catgtcatga gatgataaaa gaaagtaaaa ttggagcagc agtatcatca tctgtaacat
    43741 atcctcttac ttctaagcca gaagatgtat atgcagctag aatgaatgat aattttaaag
    43801 tgtactatat gcttgatatg catcattatg gagaatatcc agggtattat atgaagtatc
    43861 ttgaaaaaag aaatatagtt cctcacatgg aagatgggga taaagagata cttaaaaaag
    43921 ctaaaatgga ttttatagct gtaaattatt ataggacaaa ttgtgcagaa gctttacctg
    43981 aagattcaca acatccattt ggattaagag aaggaactgt tgattttagt atgtatggac
    44041 tatttaaaat gtcaatgaat cctaacttag aggcttctga gtatggagca gcaatagacc
    44101 catcaggtct tagagttgcc ttaaatgaat attggcaaag atatcactta cctgttataa
    44161 taacagaaaa tggtctagga gctaaagata tattagaaga tggaaaaatt catgatgact
    44221 atcgtataga ctatttaaga agtcatataa atgcatgtaa actagctatt gaagatggtg
    44281 tagagatgat tggatattgt ccatggtcat ttacagacct tttaagttct tctcaaggtt
    44341 ttaataagcg ttatggactt gtttatataa atagaactga ccatgaagtt ttagatttaa
    44401 aaagaatcaa gaaagatagt ttttactggt ataaagaagt tatagagaac aatggaatag
    44461 taaaataaac ataaatatat attgagggtt ttagtatgta cattacaaag gtattaaata
    44521 attcgttgct acttgcaaag gatgataatg atgaagaaat aatattgatg gggaaaggca
    44581 ttggccataa ttataaggcc gggtctgagt taaataaaaa agatatagaa aagatttttg
    44641 ttcttcatga tgaaaatata aaaaagagta ttatacaatt ggcaacagaa atagatgcag
    44701 aatattttgg tatagctcaa atgataattg catatggaat agaaaaatat aacttaaaac
    44761 ttatgaacca tatatatttg gccttaactg accacattgc ctttgcagtg agacgtttta
    44821 aagaaggtat aatgattgaa aatcactatt tgttcgaaat caaggaattt aatccaaaag
    44881 agtatgatat aggaaaatat gctattactg tttttaagga ggttcttggt ttagacctac
    44941 cagaagaaga gattggtaat atagcaggcc attttataaa tgcacaacaa gataatcctt
    45001 atagtgatag aaataagaga agtgctaaaa ttgtaaatgc tattttgcaa atagttcatt
    45061 atcacttttc tattgtatat aataaagagt cattttatta taggagattt gtaatgcatc
    45121 taaaggcctt ttcacaaaga tttctggcta atgaaccatc aagagaaaaa atagatttta
    45181 tatatgagca agttcaaaaa aattgtaagg aagagtatga atgtgtgaaa aaggtaggag
    45241 cttatatgta taaggaatac tccagagaat taccaagaca agaggagtta tatttgatga
    45301 ttcatattca taagatttta ggagaattat gcgaaaatag ttagaatatc ttaatatgct
    45361 agtaatatta taaaaaatta aaaattaact gcactttaaa tatgaataat tgcattactt
    45421 aagggggttg tcttaaaatt gattttttta attaaatttg aagctcaact ccttttttaa
    45481 acctttatat cattacaact ttaatgttta tacgactgta ctttagatgt atatctactt
    45541 tttacatttc tcaacatgca aacctaccat tttttgcagt atgttaattg aaataaacca
    45601 ttgagttaac cctaaattta ttctggactt ataggaatag ttccaaagat actttaaaat
    45661 attttctgga ataattgctt atttcacata tgttttattg tgatgttttg atttaataaa
    45721 ttcttcaact ctgtatatag taaatgtatt atcatgtttg ttttattcaa gcacacttat
    45781 aatacgcttg attttttcac aatgatgaaa tatactaatg ggtgttatag gtataagaat
    45841 cgcattacac ttgaagaagt aagtgataaa tgaggtataa atcctcatat gataaactac
    45901 tatttcattg aggaacatat tctaagtgat gtcaagatac tacaatatga ctaaattcct
    45961 agtgataaaa aagtctacga atgagtgtag aaaggggaac taagatgaat aaagttttaa
    46021 ttatagatga tgataaagat ctttgtattt tgttaaaaaa gagtatttca attgaacaga
    46081 ttgaagcaaa ctattgccat tctgggagag atggactggt tgaactttca aaacaagatt
    46141 atcagttagt agttctagat gtgatgatgc caggtatgga tggttttgaa actatggaac
    46201 aaatacgaaa atatagcagt attcccatct taatgctgac atcaaaaagc gataattaca
    46261 gcaaagtgca tggattgcga gctggtgcag atgactatct aaccaaacca tttgaaatta
    46321 cagaatttac tgctcgtgta ctgtctttaa tccgacgtta cacccgtttt aatccaaaaa
    46381 atgaggagcc acagcaactt tctttcaaag gtttgttgat tgaccttgac cagcatacag
    46441 ttactaccaa tggcgaacct atgttgcttc cacccaagga atttggtgta cttctttact
    46501 gtgcaaggca tcagggtaag attttgacaa aacagcagat ttatgaagcc gtgtggggtg
    46561 agccatatgt atatgatgac agcaatatca tggctattat cagtcgccta agaaaaaaaa
    46621 ttgaactaga cccatcatct cccatctatt tgcaaacaat aaaaggaatt gggtatcaat
    46681 tcaacacgga ggtatgaaaa tggatgaaca aacgataatt cttttcttac tcttgctgtc
    46741 gattggtcta ctttttcttt ccatatttgc tttttccaag cttgggcata tttacaagcg
    46801 cctaaaagat atagaggaga tactggctga tgtacaaatt ggagacgaaa atcgtaaaat
    46861 actaattaag ccatgtgatg ttatggctcc attggtttat cagcttaatg agattgtcta
    46921 cgattatgaa aataagttac tttctctgaa aaagtcggat aaagctagca aacaacttat
    46981 gacaagttta tctcatgatg ttcgtacacc attaacaact ctgattggtt atctggatgc
    47041 tgtacacagt ggtatagtta tcgaacaaga gcgggaggaa taccttgaaa tcgctaggcg
    47101 tagagcctac gacctcaagg attacattga tgttcttttt gactggtttc ggttgaactc
    47161 tgatgaattt acgctgtcaa tagaatctgt ggaaattgca gagttgtcac gcaatatttt
    47221 gaaagactgg attcctatat tccatgaaaa gaaactgaat tttgaaattg atattccaga
    47281 aaatcgacta atggtaaacc tcgacccaga tggatattcc cgtgtggtca ataatctggt
    47341 tcaaaatgtt cttgctcaca gtaaggctcg gcaaatcaag attaccatga gtgaggattc
    47401 cagaatggta ttactccgtg ttgaggatga tggtgttggg attgccagag aaaatttacc
    47461 acatattttt gagcgactgt ataagtgtga taaaggacgt tcagaaaaag gaagtggtct
    47521 tggtctatct attgtcagtc aaatggtgga gcgaatgggt ggtcaggttt ccgtagaaag
    47581 tgagattgga aagtataccg tatttacagt gagcttacca ttaatataaa attgtttttc
    47641 ccccatgtga catgtataca catggggatt ttttgtaagt tataaagttg caaggttaat
    47701 acaaggttgt tgcaaggtta atgtaagatt gaggtggtat gataacttta ataaacaaag
    47761 gaggctttta ttatttatga ataatttaat tattgaaaca aaaaatctca ctaagcaata
    47821 tggtgctcaa aagagcgttt ctaatttgaa tattcacgtt cagaaaggtc gcatttacgg
    47881 attgctagga agaaacggtg cgggtaagac gacaactatg aaaatgttat tgggtctaac
    47941 aaaaccaact actggagaaa ttaaaatatt tggtcaggac attcacaata acgagaagaa
    48001 gcttcttcct cgaattggat gtttgattga atcaccaggg ttttatccta atctgacagg
    48061 aacagaaaat ctcaaaatct ttgcaaggct tagaggaata ccaaaaaaaa atgcagtaga
    48121 atcagcactg gatgtagtcg gtttgccaaa caatgacaaa aagttatttt ctcaatactc
    48181 tcttggtatg aaacagaggt tggcgattgc tcttgccatt atgcatgacc cacaactgtt
    48241 gattttggac gagcctatca atggacttga tcctattggt attgcagaag taagaacgtt
    48301 tattcgtgat ctctgcacag aacgaggtaa aactattttg atttccagtc acattctttc
    48361 tgaaattgcc ttgttggcag atgacattgg cattattgac catggtattt tgttggagga
    48421 agaaagtctt gcccaattag aagaaaaaaa cagtaaatac atacattttg ctgtatctga
    48481 tgcagcacag gcagcaaggc ttctggaaac ccaacttaac ctgaaaaatt tcaaagtagc
    48541 agataattac aatattatgc tatatgatac agatgtttct gttgcagaaa taaatcgtgc
    48601 tttcattcta aacaacattg aggtatcaga atctcattta tgcgaagata ctcttgaaga
    48661 ttacttcaag aaagtaacag ggggtgaagg aattgcttga tataatttca tgtgaatttt
    48721 caaaactcaa gcggttaaaa tttatactga tttctatttt aggtgcttgc ttatttccaa
    48781 ttcccgcaac aattttgatt gcaaaggaca acttgccctt tgagcaattg ttcaaattag
    48841 ttgtcaattt tggatatttt ttgctattac caattgtatt aagtattgtt gctagccaac
    48901 tattttttat agaaagagat aatgatgtat taaaaaatct tgcaacagtg ccagtgcaaa
    48961 aaggaaaact ggctctggca aaattggctg tgttgctgtt tatatcattg ttctactctg
    49021 ttgcagggtt aggagctact atcattggag gtttcattgt tggaatagta gagggggttg
    49081 ctgtaaaatt aggaatgagc attgcttttg gaattatgtt ttttgttact gtacttcctg
    49141 ttgtggtact cattgtatat ttcaatcgca gctatatttt ttctattata ctttcttttg
    49201 tatatgctat tttcaatttt agtatatcct taaatattat caattttgaa ccaaataacc
    49261 caatattaag tgtattacct gctcctgtta taatgcgttg gtggatggca ttttggggag
    49321 atcccactgg cgaatacacg gcacttagac agccatattt gctctcaact cctgcttgtg
    49381 taggtatttt atgtttgata accattatag cagtcctgct gatttgtatg atatacaaaa
    49441 agcaggagaa ttaggaggta tctatgcttg atataattac tacagaattt caaaaaataa
    49501 aaagatataa tattctatgg attggtgttg ttgctgttct gttttcagca ctattggcag
    49561 tttttcaggt tgtaagttca cacggcagtg accctctaac gtatgagagc tttgcaaacg
    49621 gagttatctg gaataatttc agtcttagtt ttccatttgg gattacactt attggaggat
    49681 atcttattaa ccgtgaatat acagaccaaa ccttaaaaaa catcctaact gttcctattt
    49741 cattacgcaa attgctagtg ggaaaattaa ttgcggttgg aggaattact gtattgttaa
    49801 gtcttttcag cttcttatgc acatttattt tggggttgtt gttttgcaat atcaatatat
    49861 ctgttacact cgttgtaaaa tccttagttc agattttgtc tgttaatctg tattgttatg
    49921 ttgctgtttt gccgattatt gcttattttg gtcgtaaaca aaatgcattt ctgacgggtg
    49981 tagggattgc atttgtatac ggtttttgtg gtgtgtttgt tgctggaaga aatctgacag
    50041 acttttatcc aatcacagca ggtcttggtc tagttggata caacaatggt gctggtactg
    50101 tttatcagcc acttatcggt gctatgactt tgattgtaat tctaatcttg acaactattc
    50161 tacttgtatt tacacctaat tatgataaag ttatgaccat atctaagaaa aaagggataa
    50221 agcataaaaa gtattctcat aaataacata aatattgttt ctattattta agttgctaga
    50281 cagtgaaaag aaaaaatctg tcgtacagtg tctatatgct catgtattca agaagctaca
    50341 gttttgattt ttcataactg tagcttcttt ttgtatttat taaatcacga ctatcataat
    50401 acttgcaata ggtatagcga caagagagag tactgtcata tgtgttttat atattacatt
    50461 aagtatattc taaaatatat gttttaaata atatatttga aaataggaat atttaaataa
    50521 aaaaaataat attatatata gttgacaaaa attggcaaat gagataatat ataaatatag
    50581 aaatagtaat cattactatt attaaaaagg gaggaattaa ttatgaactt aaaaggaact
    50641 aaaacagaaa agaatttaat ggcaggattt gcaggagagt cagaagcaag aaataaatat
    50701 acatactacg cttcaaaagc aagaaaagaa ggttataatc aaatagctgc tatatttgaa
    50761 gaaactgcta acaatgaaaa ggaacatgct aaattatggt ttaaactttt acatgatggt
    50821 atgccttcaa cagaggaaaa tttaaaggat gcagcagcag gtgaaaacta tgaatggact
    50881 gatatgtatg caaaatttgc taaagaagct agagaagaag gatttgacaa gatagcttat
    50941 ttatttgaag ctgttggaaa aatagagaaa gagcatgaag aaagatactt aaagctttta
    51001 gaaaacttaa atgaaggaaa aatatttaaa agagatgaag aagttgtttg gcaatgtcaa
    51061 aactgtggtc atgtgtatgt aggtactgaa gcaccagaaa aatgcccagt atgtgaccat
    51121 ccaaaagcgt atttcaatat aaaagctgaa aattattaaa aattatcatt attaaaaagg
    51181 aatgttaact atgaaatttt ctaaacaacg agaactgatt ttaaatgaaa tattaaataa
    51241 tccggttcat cctactgcgg attacctata tgaaaactta aaaaaagata atccaaattt
    51301 aagcttagga actgtgtata gaaatttagc tcagctaacg gaacatggct ttataagaaa
    51361 ggttagtatt ccagggtatc cagatagatt tgatggtcga atagataatc actatcatat
    51421 catatgtgaa gtatgtggag aggtatacga tttagaatcc gaagttctta ataatttaca
    51481 agaattaata tctgaggaga cagacataaa aataacatct tataacataa gttttaaagg
    51541 gatttgtaat aattgtaaaa ggtgtagcca agtaggttag ttttcaaatt ctaaaaataa
    51601 ttaatgaggt gagtttatgt gtagtgaaca aaaatttttt atatgtgaga cttgtggaaa
    51661 ccttgtagga atgatacaaa gtggtggtgt acctattttc tgttgtggaa aacctatgaa
    51721 ggaacttgtt cctaatacaa cagatgcagc agttgaaaaa catgtacctg taattgaagt
    51781 ggatggaaat aatgttactg ttaaagtaag tagcacaact catccaatga ctaaagaaca
    51841 tcatatagca tgggtgtatc ttatgactga acaaggtggt caacgcaaat gtctagcagt
    51901 agatggagaa cctgtagtta aatttgcttt gaatgatgat gataaagtga tttctgccta
    51961 tgcttactgt aatttacatg gattatggaa ggctgaactg taatatttaa ataaattgag
    52021 tcaaaaagga ggctataaat gtattttcat acaaatagcc tcctataaat ttatataaat
    52081 tcaagtttta attttgttta atgtcaatat aatataagga gaaaataata tgtcaattta
    52141 taaatgtagt gtttgtggat atatttatga tgaatctaag aatgataaaa catgggatga
    52201 attaagtgaa gattgggaat gccctgtatg tacaaaaggt cgtagttatt ttggaaagat
    52261 aagtactgtt tactatgaag aagatgaaaa gatagcagaa gacatagttg aagatgaatc
    52321 taaattaaat actgaaaaag agggagattt aaattactta agtacatatt taagaagaga
    52381 tgatgaagtt gagaaacata tggatataat tcacgaaatg gctgttacag gaaaatctat
    52441 aatagaacct atgagaacta aactaccagt aatttcttgg gatgatattt taattatggg
    52501 tgctcaatta aatccattac ctttaaatga gcatgatgaa gtaaatacaa caacaataat
    52561 aggaaaaaaa gcaaagaaac ctatgattat agaaaatcca gtttatattt cccacatgtc
    52621 atttggagct ttgtctaaag aactaaaaat agctttagca aagggtgcag cccaaaataa
    52681 gacagctatg tgtagtggtg aaggaggtat attaccagaa gagaaggaag cttcatacaa
    52741 gtatattttt gagtatgtac caaataaata tagtgtaact gaagaaaatc ttaaaaattc
    52801 tgatgctata gagataaaaa ttggtcaagg aacaaaacct ggtatgggag gtcatcttcc
    52861 tggcgaaaaa gtaactgaag aaatagcaaa agtaagaaat aagcctgtag gtcaagatgt
    52921 aataagtcca tcttgttttg aggaaataca atccaaagaa gatttaaaga aacttgtaga
    52981 tgaacttaga gaagtttcag aagggcgtcc aataggtgtt aaaatttcag caggtcatat
    53041 tgaaaaagat atggaattta tagcttatgc taaacctgat tttgtaacta ttgatggtcg
    53101 tggtggagct actggagcaa gtccaaagtt gcttaaagat gcaacttcaa ttccaacaat
    53161 atttgctctt tatcgtgcta gaaaatacat agatacacat ggattagata tagatttggt
    53221 tataacaggt gggcttcgta tatctactga ttttgctaag gcaattgcta tgggtgctga
    53281 tgcagttgct attgcaagtt ctgctcttat ggcagcggcg tgtcaacaat atcgtatatg
    53341 tggttctggt aagtgtcctg taggagtagc tactcaggat gaggaattga gaaaaagatt
    53401 acatatagaa aacagtgcta atagagtggc aaacttttta aatgtaagtt tagaagaact
    53461 taagactttt gctagaattt ctggtcataa agatatacat gatttaagtg ttgatgattt
    53521 atatactgtt aactctgaaa tatcaaatta tacaaacata cagcatgtat agattgaagt
    53581 ttgaaactat tagattttag atagctataa aagtaaatga taaatatatt ttataaaaga
    53641 gagtgtctta aaatgaactt tttagttcat aagtcactct tttttttatg aagccaaata
    53701 tatttatcat tttagcaatg aaaaagaatc ttatctcttt attttgagat agactccttc
    53761 tatttatgaa aattaaagat attaaattgt aaaatattct gtgatgtcgt actattaaac
    53821 taaagattct gtactttaaa gtaaataaaa atttaaggag tgattattat ggaaagattg
    53881 aagatacaag ttttagttga caataatact tatattgata gatactttgt tggagaacca
    53941 gcagtaagct attatattga gattgatgga aataggattt tgtttgactc agggtattca
    54001 gatgtattca tatcaaacgc tgaaaaatta aatatagatt taggaaattt aactcatgtt
    54061 gttttttctc atgggcataa tgaccataca agaggtatcc agtttctaga aaataggtat
    54121 gatttatcta cagttgaact tatttcacat cctaactgtt ttatacctag aaaacatgga
    54181 gagaaaagca taggagctcc attttctgct gaggaaatta aaaatatttt tatgtataat
    54241 cctaaggata agccatttaa tctaagtaag aattgtgtgt ttttaggtga aataccttct
    54301 ataaatgatt ttgaaaaaag agctaaaatt ggtaaatgta aaataggaga tttgtgggaa
    54361 gatgattatg ttttagatga ttctgctata gtttgtaaaa ctgataaagg tatttttatt
    54421 gttacaggat gttctcatag tggaatttgt aatatagtag agtatgctaa aaaggtatgt
    54481 ggagatgata gagttattgg tatactaggt ggttttcatc tttttgaatt gaataatagg
    54541 ttagatagta caatacaata tttggataaa gaagaaatga gaatgttgta cccttgtcac
    54601 tgtgtttctt ttaaggtaaa agcgaagatg aatgaaatat tgaatataaa tgaagttgga
    54661 gtagggctga ccataagtat atagtatttg aaagtatata agatttaagt tgaaggttaa
    54721 aaagatattt tctatagaaa atatcttttt tgttacgttt attttaagag aaatcaatat
    54781 acttttatat gaaatattgt aaaaaaattt attt
//
LOCUS       DAMJZE010000007        51878 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJZE010000007 DAMJZE010000000
VERSION     DAMJZE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.007"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(64..2127)
                     /locus_tag="PC541_000321"
     CDS             complement(64..2127)
                     /locus_tag="PC541_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2837809.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2508..5843)
                     /locus_tag="PC541_000322"
     CDS             complement(2508..5843)
                     /locus_tag="PC541_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2837810.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5893..6321)
                     /locus_tag="PC541_000323"
     CDS             complement(5893..6321)
                     /locus_tag="PC541_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837811.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6311..6691)
                     /locus_tag="PC541_000324"
     CDS             complement(6311..6691)
                     /locus_tag="PC541_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2837812.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6724..7896)
                     /locus_tag="PC541_000325"
     CDS             complement(6724..7896)
                     /locus_tag="PC541_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2837813.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(7900..9363)
                     /locus_tag="PC541_000326"
     CDS             complement(7900..9363)
                     /locus_tag="PC541_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2837814.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(9873..10742)
                     /gene="gatY"
                     /locus_tag="PC541_000327"
     CDS             complement(9873..10742)
                     /gene="gatY"
                     /locus_tag="PC541_000327"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2837815.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(10762..12678)
                     /locus_tag="PC541_000328"
     CDS             complement(10762..12678)
                     /locus_tag="PC541_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2837816.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(12681..13601)
                     /locus_tag="PC541_000329"
     CDS             complement(12681..13601)
                     /locus_tag="PC541_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2837817.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13703..14455)
                     /locus_tag="PC541_000330"
     CDS             complement(13703..14455)
                     /locus_tag="PC541_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2837818.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14847..16292)
                     /locus_tag="PC541_000331"
     CDS             complement(14847..16292)
                     /locus_tag="PC541_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2837819.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(16314..16667)
                     /locus_tag="PC541_000332"
     CDS             complement(16314..16667)
                     /locus_tag="PC541_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2837820.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16696..17943)
                     /locus_tag="PC541_000333"
     CDS             complement(16696..17943)
                     /locus_tag="PC541_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2837821.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(17951..18268)
                     /locus_tag="PC541_000334"
     CDS             complement(17951..18268)
                     /locus_tag="PC541_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2837822.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            18510..20423
                     /locus_tag="PC541_000335"
     CDS             18510..20423
                     /locus_tag="PC541_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2837823.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20546..21700)
                     /locus_tag="PC541_000336"
     CDS             complement(20546..21700)
                     /locus_tag="PC541_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2837824.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(21979..24594)
                     /gene="mngB"
                     /locus_tag="PC541_000337"
     CDS             complement(21979..24594)
                     /gene="mngB"
                     /locus_tag="PC541_000337"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2837825.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24738..26663)
                     /gene="mngA"
                     /locus_tag="PC541_000338"
     CDS             complement(24738..26663)
                     /gene="mngA"
                     /locus_tag="PC541_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2837826.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26793..27518)
                     /locus_tag="PC541_000339"
     CDS             complement(26793..27518)
                     /locus_tag="PC541_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2837827.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27781..28560)
                     /locus_tag="PC541_000340"
     CDS             complement(27781..28560)
                     /locus_tag="PC541_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2837828.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28553..29980)
                     /locus_tag="PC541_000341"
     CDS             complement(28553..29980)
                     /locus_tag="PC541_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2837829.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            30371..31093
                     /locus_tag="PC541_000342"
     CDS             30371..31093
                     /locus_tag="PC541_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2837830.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            31290..32957
                     /locus_tag="PC541_000343"
     CDS             31290..32957
                     /locus_tag="PC541_000343"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2837831.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(33053..34372)
                     /locus_tag="PC541_000344"
     CDS             complement(33053..34372)
                     /locus_tag="PC541_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2837832.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34413..35159)
                     /locus_tag="PC541_000345"
     CDS             complement(34413..35159)
                     /locus_tag="PC541_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2837833.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35403..37412)
                     /locus_tag="PC541_000346"
     CDS             complement(35403..37412)
                     /locus_tag="PC541_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2837834.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37850..39283)
                     /locus_tag="PC541_000347"
     CDS             complement(37850..39283)
                     /locus_tag="PC541_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2837835.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39370..41286)
                     /locus_tag="PC541_000348"
     CDS             complement(39370..41286)
                     /locus_tag="PC541_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2837836.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(41360..42220)
                     /locus_tag="PC541_000349"
     CDS             complement(41360..42220)
                     /locus_tag="PC541_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2837837.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42571..43758)
                     /locus_tag="PC541_000350"
     CDS             complement(42571..43758)
                     /locus_tag="PC541_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2837838.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43772..45163)
                     /locus_tag="PC541_000351"
     CDS             complement(43772..45163)
                     /locus_tag="PC541_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2837839.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(45758..47458)
                     /locus_tag="PC541_000352"
     CDS             complement(45758..47458)
                     /locus_tag="PC541_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2837840.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(47430..48833)
                     /locus_tag="PC541_000353"
     CDS             complement(47430..48833)
                     /locus_tag="PC541_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2837841.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(49097..49588)
                     /locus_tag="PC541_000354"
     CDS             complement(49097..49588)
                     /locus_tag="PC541_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2837842.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49614..50426)
                     /locus_tag="PC541_000355"
     CDS             complement(49614..50426)
                     /locus_tag="PC541_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2837843.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50439..51398)
                     /locus_tag="PC541_000356"
                     /pseudo
     CDS             complement(50439..51398)
                     /locus_tag="PC541_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(51516..>51878)
                     /locus_tag="PC541_000357"
     CDS             complement(51516..>51878)
                     /locus_tag="PC541_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2837844.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRSISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT        16579 a         9531 c         5485 g        20283 t
ORIGIN      
        1 agaggtgcta ttttattttt aatataatta atattctatt acgcaaaaaa tgcatatatt
       61 tcattatttt atggaaaata ttacttcatt taactctata tcaaactcat taagtaaaaa
      121 tctaattata tttaatttag cctctgtatt taaatttgtt tctaaatata tattttctga
      181 tattttaata ccttttctta atttatatgc atctctagac ttaatattat ctataaaatc
      241 tcttttataa gttttgtcta caatactgtt aaaccttaca gaatccaaat catataaagt
      301 aagagcaact tgttcataaa attctgtcca atttttagcc ttatatgact ctcctaatat
      361 actaaatgat actatacttt catttgtaaa attaacatca tcatcctcta acgtaaatat
      421 attttcactg ttacttggag gttcatatgt aacacttgga tatttccaaa tttctacagc
      481 tctatctagt aatatcttag ttctattttt tatttcttct tcattccatt tatctataga
      541 tgaaatatat ttatttagat aaagcctact atctttaaat ccatcttcca tttctttctt
      601 ttcttcaaat gttttattac ttagttttga attatatcct gtcaaagtta aatttccaat
      661 tgtatgtaaa tatttatcat gtatctcctt atagttatct cctagtgatt ccttccattt
      721 atttgttaat gtctgtggca ttatatgctc tatatttagc atattattat tgattaaatt
      781 ttctaaatca acaatttctt tattactata attctctaac ctctcaagta tatataactt
      841 attctttgac ttagtattat atatatcttt agtttcaaaa gcatttgaaa attcactgtc
      901 agtaggaaat ctttgagatg atttcttatt tataaaaata tatcttaata tattagaata
      961 gttttcttta taatcactaa attttttaat ttcccttcct aagttcataa atactttatt
     1021 cagtgcattc ggaggtacat cacatactat tcttcttaat ataaaacatt ctactaaatt
     1081 aagggattct acaagtactt ttttatttat tataccttca ttataatcat caaataattc
     1141 taatataaac ggatatgata caaatatttc aagtttgttt atccttttca aaactttgtt
     1201 tacatcatta tcatcagtac atgaataaga tatctctcta taatagttag aaaatttaag
     1261 taaatctttt aataaaattt ctatatctat accattatct gatacatacc ttttaaaact
     1321 aaaatatact ttatttttat tcggtatact tctctccttt atagtcaaat agtctcttat
     1381 aaaactactt acgttgctac ttgtattttt ttcaatttta taccaataat tattataaaa
     1441 ttcctcttgt aacttagagc ttctacccat taacacaaag tttcttactc tatcagcttc
     1501 acttaaatct aagccagttg aatttagact ttcaaagatt aattgagggt catcttcacc
     1561 attttttaac tctatttcta ctatagttaa tttctgtata gcatcaaaca aatcatctat
     1621 tgatatatta tcttctttta ttctattata aaaatataaa taatttgaaa ctatattaga
     1681 atttctatct gtattcattt cttcttcaaa tagttcatag tacgcttttc tatcttctgc
     1741 tattggtttt aactttattt ttccatcttt atctcttgag tgcttgttta taagataaca
     1801 ctccttaatc tgttcttctg ttgtaataat tccttttgat tccccaaact cttttattat
     1861 ttgacacatg gcaatcaata atattgacac agtagtcact ctttgttgtc catctattat
     1921 taaatattct tttcccatac tatatataga tacaactgac cctaagaaat gagttctaaa
     1981 tccagaatct attatatcct ctaaatcatc aaacaatctt ttacattgtt ctttcttcca
     2041 gttataattt ctttgatata caggtactac aaaacttttg tttgacccct ctaaataact
     2101 aagaaattca atttctcctg ctttcatatt aactccccct ataagttaaa taaattccat
     2161 tcataactcc atgtatctat atattatatc aaaattttga ctcataaatg tatatgaaag
     2221 ccttattata taccttttta tacctgtttt tattttatta aagtaaatac atgattattc
     2281 tataaaatat acaaatactt aattttaaaa attattattt aatcttcttc atgagtaatt
     2341 catagttatt tatcaattaa aaagagatat tgcataatta ttcaatacct ctctttgtat
     2401 atttacatta tccatatcta gttatctatt tcaacccaat attagactac ctactaataa
     2461 atttattctc tacatcatta acaataattt tgcactaaat aacatcatta gcctatctct
     2521 gatgcatttt ctttaatttt atttatagtt ttaatcaatt tatttctctc ttcaatatga
     2581 ttttcaaata aatcaattat acttccaaca gttctaaatg ggtcttccat caacactttg
     2641 ttatcctcta taaatccatt cttgactaca taatcgacta taagtttaac aaaatgtatt
     2701 tgcttagtat taaagctctt attatttaaa aactctgaaa aagcttcatt tgcagcttct
     2761 ctatccaatc ccactagtct tctcactaat tgacttactg gagtatctcc aaactccttc
     2821 ttataatcat cacaattacc taactgtttt aagagtattt cctccaacgt ttcaacatct
     2881 tgctttgtta tctttttatt atttcttaat ttaaatattg ctaattcatc tttatggcta
     2941 tttaaataat actctacctt ctttttataa ttcttaagat tgtttgcatt atatacagaa
     3001 tcatttctat cttctgctac aatcatatct tcaaaacttg tataatatat cttttgacta
     3061 gacttttcaa gatacttaat aagctctctt aatgatatac gtacttcttc tacctcaaat
     3121 atatctgaat cttcccaaaa attctctgtc attgcttttt caatagtata tttttgttgc
     3181 tgaatctgtg gtatagtccc aagttttgaa agtttttctg ctgtagccat tacatgcctt
     3241 atacctttac ttgcattatt attttgtaag tatgcaagtt gtatattata cataactaca
     3301 tcaaatcttt tagccaattc atcatcacta tatgaagtta tgagtggtga tatatattct
     3361 cttatctcat tttgtgctac tgctccaata ttgctccaaa cattttcatt tttatactta
     3421 tgtacataac ttaaattcat cctaactata taactatcct catttaaagc ttttacagaa
     3481 gttataagtt cttctaacaa ttcttttcta agttctgcat actcaccttc tatatattta
     3541 atatcttgaa gttctttaac catacttacc tttatattaa ataatttttc agtaagtgtt
     3601 tctgctttat ttccctcaaa tcctttagga ttaaatttaa aaaattcaaa attattacaa
     3661 aaatcaaata ttaagaatct ctccttatcc atatcaacac ctaataaatc tggacataat
     3721 ctagttcctc tacctatcat ttgccaaaac ttagtcttac ttctcacttt cttgaaaaaa
     3781 acaagattta gtatttcagg tatgtcaatt cctgtatcaa gcatgtctac tgaaactgct
     3841 atttgaggta atttattctt atcagaaaaa tcatctatta tagaatctac ataatttata
     3901 ctatagtcaa ctgcttttac aaagttccca ccatactttg gatacagctt attaaatctt
     3961 tcaactattg ccctagcatg ctttgtattc tttgcaaaaa ttatagtctt accaatcttt
     4021 tcttctcctt ctattttcaa cccctctgtc attagcttat ttaaaactaa atctattgtg
     4081 tcagaattaa atagccactc atttactgca ttattcccta tttcgtctcc aatactctca
     4141 tcatcattaa atgtattttc atattcttcc ttctcttcat cagaaagctc atcatactta
     4201 attccatctt ccataatctt agtcttaaac tcaatactag tataatctac taaatatcca
     4261 tcctcgactg ccttattata ctcataagca tatgtaggta ctccattttc catgtcaaaa
     4321 actgaataag tatttttatc tatctcatcc tttggtgtag cagtaagacc cattaagtaa
     4381 gaatcaaaat aatcaaagat tgacttatac tttttataaa tacttctatg cgattcatct
     4441 attataatta aatcaaaatg accaggtgta aacaatctct ttccatcctt agacttcgta
     4501 tcatcaattg cattcatcat tgttggatat gtagaaaata tcatcctagc atcttctggg
     4561 ctgtctttgc tatccaataa attacataac gccaagttag gtaatagctt agtaaaactt
     4621 tttttagctt gctttaccaa tgcttttcta tctgctagaa ataatatatt tttaatccaa
     4681 ttatgtcttg aaagtacatc aactatagat atagcaattc ttgtttttcc tgtaccagta
     4741 gcacatacca aaagagtttt gcgttgctta ttttcaaggg cctcacatac agcccgaata
     4801 gcttcttttt gataatatct attggatatt tcatcattta ttgttacaga acttaaagac
     4861 ttcttactct ttcttctatc catcataagt tgtaattctg attttttata aaatccataa
     4921 acttttctat ctgaatagtc atcccaaata tacatatcaa aaccattagt caaaaatatt
     4981 actggtcttt gactatatct cttttccaaa caatctgcat ataatttagc ttgttgttga
     5041 ccaatttttg ggtctttgct cgttcttttc gcctcaacta ctgctaatgg cttaccattt
     5101 tcaccataaa gaacataatc tacatatcct acaccacttt cattaggcat accttgtact
     5161 tctatttcct cccctatatc ttttccaaat tcccaacctg caagctttaa ttctatattt
     5221 atatatcttt ttcttgtctc aaattcgctt acttcgtcta tcttgaaatc atagtttaca
     5281 gtattaactt ttctcttttc agttagtgac tttcttaatt cctcattttc ttttattact
     5341 tcttcaagct ttttatcttt tgaacttaat ctctcgtaaa gatttttaag ttcgtctggt
     5401 ctcgtccttt tctcttctcc atgaagtaat aaatcttcat taaagtcttc tgctgtatat
     5461 tcatctgaat aacaatagtc tatccaacta acaaattgat gcaaattgtt aagtgaaagt
     5521 attgcttctc ctctctctat gttcgcacta gtatgtacag ataaattacc taacttaact
     5581 atatatttta ataaaggtaa catatcatag tctataagtt ctataaaact attattatga
     5641 atcaacgtac tgagattatc ttgataaggc aatactaaat cactatcatt agcatatagc
     5701 cattttactg caagctctaa agctcttctt gttatagttg cacaagtaga tggactaact
     5761 agtatacttt tttctgcttc taagcaagca gtattaaaac tattgaaatt tttcttcttt
     5821 tgtaaaaaat caaaattacc caaggctttt atcccccttt tgtcccttag ttaaaataaa
     5881 tctctctaaa aactatttct tacagctgtt tcttatacta acaaatactt cttcattttc
     5941 tgtaaacaat tcgtacatat cttcaaactg tggtatatca tatccatgag aatcatcatt
     6001 tctcaatttt ctaagtgttt ctataactat agaagaattt ttaggtaata aattgtgata
     6061 ctcacaagct aaaaggtagt cataaaagtt tttagatttt atgtttaaat caattgattt
     6121 ataatacaac attgcttttg ttaattctag aaatgatacc atatagcttt taaagccttc
     6181 ttttatgtat ctagcccata actcaggcat ttcttcctta tcatataaga ttttgaagtt
     6241 ttgcaggtca tacaagcaag aaccaaaatc cctatttact tcttctattg agttccaaga
     6301 taatttgttt ctaattacca tacatgtccc tccaatctaa gatatctatt acatcactgt
     6361 tttcaaaata gaatttattt atataatcct taaaatcttt agattcataa ataacttcac
     6421 cttctaaaat agctttttta agtgctattg tataattatc tgtaacaatt tttaggtcta
     6481 catcaatgtc taatatattt cttagagctt ctgtcttagt tgcaagttct gtaattggta
     6541 cttttttgat gggaatccaa gctatgtcta tatcactttc tgatgtatgt ttatctgttc
     6601 cataactacc aaatattatg attgctagag tattttcttt tggaaataca tttattattg
     6661 agtttttaat ctcatttggt atatttctca tttaatcacc tcttgtttat taggtaatat
     6721 tgtttagtta aataattctc ctttgaaagc tttttgcatt aatgaattaa agttatcttc
     6781 taactctttt aagttggttt ctatttttaa ttttaattta tccacttgct taacaaactc
     6841 agaaaactga ttttgaaatt caattggagg aaccattatt aaatattcac ctaacttact
     6901 aactgataac tcaatttgat tcgttgaacc attaacacat tttctatata ttgtgtcttg
     6961 tatatttgta aattcaaata acgctgcaaa ataatctgaa tttaagttat ttgaaatact
     7021 tcttaaaact gtaacatgac tatctgctat atattcagaa ttatcaattt tgttaaattt
     7081 tacacatctt cctaatgtcc cagttccagt agaatttatt aatatatcat tttgtttcaa
     7141 aaatatatct tctatcttct cttttaagtt ttcatcataa tatttacaat tttctacctt
     7201 caaatttctc cagtatatac atttttgatt tataactttt atatttgatt gctctacata
     7261 tttaggagat acacctcttt ttaaatatat agaaatctct ttaatcttct tcatatcaaa
     7321 tttttttgta ttattaattg gctccccaaa catctcaata aatcttgatt taactaattc
     7381 atccaatgct tctatttgct cttttctttt atttattaac tcttgagctt tatttaaaac
     7441 ttctaccatt ttattttgta tacaaattgt gaaatatggt atttttatat catttaactt
     7501 gcttttattt aaagttgcac ccataactgc tctatcagta ttattaaaat ttaaagttct
     7561 caaagcataa tataaatatt cagatgttat aagttcatta ttttttattt gaaagttagc
     7621 aattgcttca tttgtaaaca tgtcttcttt taatatggca actctaccta tagacaactt
     7681 aaaactcatt acaacggtat ttttaggtac taatttcata ttagcttttt caacagctaa
     7741 atctgttatc tcttcttttg attttgatat atatttttct ttcaaatcag aaattgataa
     7801 ccatctatta ccacttcccc aataatcaga agtatttcta gatggtgttt tgccaatatt
     7861 tatatagcac aattcattta attttatata ctccataccc tatacctcta acattctttc
     7921 taattcttct aatccaacag ctatttcctt ctctaactcc ctaactctac ctataatcac
     7981 actaggagca tcataaacta cttcctcata cactacctct ttatacttat taatagataa
     8041 atcataccca ttctctctta tctcttccac atcaacaaaa aagctttgtt ctgttctttt
     8101 tctatctact tccccatcaa gattatcaaa tctcttaata atatcctcta tatcactatc
     8161 ctttatctca tttctcttat catccaaaga ataaccatca gctttcatat catagaacca
     8221 taccttatca gttccacctg tccctgtttt agtaaatatc attatagctg tagatactcc
     8281 tgcataaggc ttaaacactc cacttggcat tgatattata gcttccaatt tatgattatc
     8341 aactatttct tttcttatcg atttatgtcc acctgttgaa ccaaacaata ctccatctgg
     8401 cactatacat gcgcatcttc caccaatttt taaaactctt aaaaaaagtg ccaagaataa
     8461 taactctgtt ttctttgtct tagtcacttt taataaatct gctgacactg cttcataatc
     8521 taaacttccc ttaaaaggag gatttgctaa caccaatgta aacttatctt tatctgtatt
     8581 aacctccgat aaagaatcct tatactctat attaggattg tcaacgccat gcaacatcat
     8641 attcatagca cctattctaa gcatagttct atccatatca aatccataga acataccatt
     8701 attaaaatgc tccttcagcc cttgcactaa gaaaagactt gaatgattat cccttaagta
     8761 ttgttgtgat gatactaaaa aacctgctga acctgctgcc gggtctacta taatatcttc
     8821 tggtgttggt ttaataagtc ttactatcat atctattatg tgtcttggag tcctaaattg
     8881 accattagta cctgctgtag ccactttgga taataaatac tcatacaaat ctccctttgt
     8941 atctctatcc ttacttaatt ctaaattact aataccatca actattttag ataacataag
     9001 aggcgtagga attttaaata ttgcatctcc catgtacttt gcataagctg agtctttatt
     9061 tccatgtaat ttttttatga aaggaaacac ttcattttgt actatttcat acattttttc
     9121 tgaatttcct tcattagaaa acttactcca tcttaaatgt tgatgttcat catcaaatat
     9181 cctttcaaaa gaaactccca aaagagttgc ttctgcttca ttttttgttt ctacttcatc
     9241 caatccttta ataaataaaa gatatgtaaa ttgttctata acttctaatg gattagttaa
     9301 tccaccagtc caaaatgtct cccaaatctt gtctactttg ctccttaatt ctcctgtaat
     9361 catatatgac tttccctcca gtgttcataa aataatttaa atatatattt tagtcgtacg
     9421 ccttaataat tagtgtctta tattaaattc aaaattttac tacacagtta ttaaattatt
     9481 tttcctattt acatgtttta gttaaaaaac taatgcaata ctcttatttt atgattatta
     9541 tacatcccat acaaaattat tttatcatat atccagcata gaagtcatct acctggataa
     9601 ctcttaccat attataactt tttaatatta atttaaactt agaaagaaat tttttattta
     9661 attatatcat cttccttatt tcaaatttct caattacaat ctcaatcaaa aaataaaata
     9721 agatgctgtc tcaaaataga tataatcttc ttttttattt tcggaatgat gaaaatatat
     9781 ttaatttcat acaaaaaaga gtgcctcctg aactaaaaag ttcattttga gacacccctt
     9841 aatttaatct tacttattct ctccctttat tattaacact tcactatctc tattgctttt
     9901 cctacacatc cacaaacttc catcctactc ttaacaaaat ctttaactga ctctaaacct
     9961 tttttaccat atttcttagg gtcaattacc tctgggtcag aattaagcac atccttaacc
    10021 ccattgctat aagcaatcct taattcagtt gcataattaa ccttgcaaat ccctcttttt
    10081 atagattcct taataacttc atctggtatt ccagacccac catgtaatac taatggaact
    10141 gatacaacat ctctaatttc agataatcta tctaaatcaa gctttggctc tcccttatat
    10201 aatccatgtg ccgtacctat tgctactgcc aaagatgata cccctgttct ctctacaaac
    10261 tcttttgctt ctaatgggtc tgtatatgct cccccatcac caccatctaa atcgtcttcc
    10321 ttacctccaa ctttacctaa ttcagcttca acaggtatat ctgaaggcat acatgcatct
    10381 acaacagatt tagatattga aatattatct tcgtaacttt catgagaacc atcaatcata
    10441 atagaagtat atccaactct aagtgcctgc atagctagtc caaagcttga accatggtct
    10501 aagtgcattg ctactggaac acttgccttc tctgatgcta cctttacatt cgctaaataa
    10561 taatctagcc ctgcatactt tattgttgaa ggtgttgttt gcaatattac tggtgattta
    10621 agttcttcag cagcctctat tactgccata accatctcca tattttcaac attaaatgca
    10681 cctactgcat aatgtccttc ttgtgcatct aataatattt gtttagttgt cactaatgcc
    10741 ataatttctc ctcctatatg tttatactga tttgatttct tcgttattta attttgctac
    10801 tatttctcta cttgttttgc tatttaataa ctcatttaaa aattcttcat tcattaattt
    10861 tgaagatagt tgagataatg tttttaagtg taaattatca ccattatctg tagctgctat
    10921 cataaatata atatgtgcta agcttccatc ttctgattca aagtcaaacc cttcttttga
    10981 tacacctact gctacacgtg gtacctttac tgcatctgtt tttgcatgtg gtatcgctat
    11041 ccccatacca aaacctgtag atgatatttc ttctctttta aggatttctt ctttaaactt
    11101 ctttttatct aaaagaacac tagatttatc aagtattcca atcatttcat caattacatc
    11161 tgttttattt tttcccttaa catctaaaat tactgtttct tcgctatata aagagatatt
    11221 attagatacg ttttccgatt ctgcactttc atttttatgt tcatcttttg ttacatttgt
    11281 attttctata gtatttttct catttactac tttaggtgta accatcatat aaagtacagc
    11341 acctactaat gaacctacaa atatacaccc tacccataaa agtggcttat tgactaatcc
    11401 tagaaccaaa aaccctccat gtggagctgg tacttctatt ttcattatat aagttaaaat
    11461 tgatgatatt gatgatgcaa tcataagtac tggcaacact tttaatggat tttttgctgc
    11521 aaaaggtatt gccccctctg ttatatgagt actacctagt atataattga ttaatcctgc
    11581 tgctctatct tcttctgtaa atctattttt aaataaagta gatgctattg ctattaccaa
    11641 tggtggtgtt atacaagctg cagatacacc tgccataaat aaataatttc cctgtcctag
    11701 aagcatagtt ccagttacat atgctgcttt atttactgga cctcccatat caaatgctga
    11761 catacatcct attactattc ccaaaattat agggcttgaa ttttgtaacc ccgatagaaa
    11821 gttcatcata gcattgttaa gtgctgaaca tggcccacca agtaacaaca ttgctacacc
    11881 cacaagacct acgctgacaa taggaattat aaaaatcgat ttaagacctt ctaattgtcg
    11941 tggtaatttt cttaacaaat gtacaaatag tttaactaag taacctgcta aaaatcctga
    12001 tatgattcca cctaaaaacc ccgaacctgt atcaaatgat atcattcctc caacaagtcc
    12061 tgctaacata ccagctcttc ccgatataga ttgagctata aatgctgcca atacaggaac
    12121 catcaagttc atagaatatc caccaacaga ttttaatgta gctgctattt gattatattg
    12181 ttctgaattt gggtcaaatg aataaatccc ccataaaaat gatatagcag taagtactcc
    12241 acctgctact actaaaggta acatgtgaga tacaccattc attaagtctt tatacaactt
    12301 ttgaccaaac gataaatttc cttcttctgc aattttactg cttgataagt ttccttttct
    12361 aatagctact tttccatcaa tacatctttg tataagttca ttagctttat gtataccttc
    12421 tttgacaggt acttctatta ctggctttcc attgaatctg tccatatcaa catttttatc
    12481 acaagcaact attattgcat ctgccatttc gatatctttt gctgttaact tattttctac
    12541 acctatagct ccattcgttt caacttttat gtcacaccct aattccctgg ctgaattttt
    12601 taatgcttct tctgccataa aagtatgagc tattccagtt ggacaacctg ttacccctag
    12661 aatcattttc tttcccattt ttataccacc tttctgactc ttatattttt tatataatct
    12721 tccacattct ccaaattacc tataccatta ctttcagcca cacttgctcc tgttgctgct
    12781 gaccttttta atgcttcttc aatatgttct ggattttcca accatatact taaaaaagca
    12841 gctaatgcag aatcaccagc acatgcagag ctgactactg taactggttg agcactagca
    12901 taataaattg attttccatt ataaaaataa gaaccttttt ctcctaaagt tagcaatata
    12961 ttttgagcac cttttaaatg tagtaattta agtacatcct taatatcttc ttcatctctt
    13021 acaataattc caaatatatc tttgatttct tcatcatttg gttttatcaa atatggatta
    13081 tactgtagta actcctttaa ttttggtgaa cttatatcga gtattgtctt tatactttta
    13141 tttttacaaa ttttaaatat atcctcatag taattatcat caataccagg aggtagactt
    13201 ccacttattg ataagtaact tatatctgct aatgactgta tcttatctag catatctagt
    13261 ttttgttttt catttacata tgacccagag tttacaaatt taaattcatc acttccatca
    13321 tttaaaaata tatttatacg agttgtatct tctacccaag taggcaatac gtcaaatcct
    13381 ctcttttcgc tttcttcaac tatatatttt cctgaaaatc ctccaaaaaa tcctaatatc
    13441 ttagattcta taccataatg acctagtaca aaagttacat ttaatccctt tccattagga
    13501 gaatatacag catttgatgt acgatttact aattttctct ttatcccatt agtagatata
    13561 ttcatatcta ttgcaggatt tgtagttaat gtatatatca ttgtcttccc ccttgcttgt
    13621 gctgtgtatg ttttcattat aataaaaaag tctatgaaaa tcttttcaca aatagaaaat
    13681 cgattttcat agacttttca atttaatttt cagaattatt atatctgacc atataatcta
    13741 taattatcct agatataata tacaagggca atctggaaga tacttcatat tcagaaattg
    13801 atatatgttc atgtttgtat cctacaatat ataaatccga atactttata agttttgcac
    13861 tttcactaca acagcaagtt ataaccttag ctccaactaa attggcattc tgtgctgatt
    13921 ctacaagctc tatggtattt ccattcaatg aaaatataat tataagttcg tcttttttta
    13981 ttgatttaga ttttattttc attatgttgg ggtcatttat aaaaacaaca ttaaaatcaa
    14041 gtaattgcaa tttaagacta aactcttttg ctacatattc agtaagccct cttgctaaaa
    14101 tatatatctt actagattta tttatagttt ttattatatt gattagtgta gttaaagata
    14161 tttgttctat aactctctgt gcttctataa aggatttatt tattatatcc cccatatttt
    14221 gtatatcatc attttcatct tttactgtta atctatacct aagttcattg aatccattta
    14281 aaccacattt tctaattgct cttgaaacgg ttgctggaga tgaatatgtt tcatgagcaa
    14341 tctctactat agataattct ggtaatattg attcattttc atttataaat ttaattattt
    14401 caagttccgt cttcgttaga ttttcagaat tacttttgtt tagctcaatt atcatatgtt
    14461 atctcccctt atataagttt tatttgtcta aatttatcga gtaatataat atattacctc
    14521 ttaactactt atttttatat acaattaaga tttagtcaat gtctatatat gtaaatttat
    14581 aatataataa ttttttgctt aaagtttatt atgttatctt atagcatttt aaaataaaaa
    14641 agaattgtac ctctaccaaa atttggatga agtacaatag atattcctca tactgatatg
    14701 tttatgatta aaagtataaa tataaatttc gctatgaatc atataattaa taaatcattt
    14761 tatttacaat tataaccccc cttataaaac tatattaata atatatacag tatttataag
    14821 gggggttgtt tatatttaaa aactatttac ttttctctta attcttctcc attagttctt
    14881 attacatttt gataccaata aaaactatct tttttataac gttctttact tccatttcca
    14941 ttgttatcta tatccacata tataaatcca tatctttttt ccatttcttg agatgaacaa
    15001 cttactatat ctattggcga ccacatacaa taagctaata tttcggttcc atcagctata
    15061 gcttcaccta tttgttctat atgttttcct aaataatcta ttctgtaact atcatgaact
    15121 tttccatctt caaatttatc atgcattcca aatccatttt cagctatcat tattggcttt
    15181 tggtatctat cataatattg agataaagta ttgtataatc cttttgggtc aattgcccac
    15241 ccccaaggat ttgcttttaa atatggattc tttatattgt cagttggatt catggtgttt
    15301 ttacttgctg atactgttgt agaataatag tatgataaag ctagaaaatc catagtattt
    15361 tcttttaaga ttttatcatc accttcctcc ataactatat ttatgttatt ttctttgaaa
    15421 aatctcttaa tatagactgg atactctccc ctaaaactta catcagtgaa aaaatattgt
    15481 agcctatttc tttttaaagc aaatactaca ttttcagggt cacaatcttc tggataagat
    15541 gtacaatctg ccatcatcat acctatcatc atatctgaat ttatttcttt tccaagcttc
    15601 ttagtcaaag ctgtagctat gaattgatga tgaatagctt gatatctagt ctcttctaca
    15661 ttatcaactt ggtctacaca aaatgcaact gaattaaatg attcataatg tattaaattt
    15721 atttgattta taactatcca atatttaact ttatttttat atctatttag tattaattct
    15781 gcatatctaa caaagaaatc taccactttt ctattagacc atcctttata ttttaatact
    15841 aaattcaaag gagtctcata gtgaagtagt gttacaatag gttccatacc attatctatt
    15901 acttcattta taagattatc ataaaacttt aatccctctt cattaggctc taaatcatct
    15961 ccatttggaa atattctagc ccaatttatt gaaaatctaa aacactttaa ccctagctct
    16021 ttcaaaagct ttaaatcttc tttgtatgta tgataaaagt ctattccact acgcttagga
    16081 taatatccat tattatcttt catagcaaac tttattcttt ctatagtcat atcagactca
    16141 taaggtttag atatatctct atctttatca tacatatgta catcagctaa acaccaacct
    16201 ttgccacctt cattccatgc accttctaat tgattagctg ctacagctcc tccccataaa
    16261 aaacctttag gaaacttacc caatttaatc actccttatt ttatttattt aaatcattta
    16321 ctattattta ccttatattc tatagacttt actattccta ctaaagacat agttatattt
    16381 aactcgctga atatggtcat aagagtatct tgagcatgtg caaataacag cgaatgtgct
    16441 cttttctctc caccagcttc cccttgtata atcttggttt gagctctatg tgcttctgtc
    16501 ataaaccctt ttgctttagc tagttttaaa tcagcatttc tataatcaaa cttttccaaa
    16561 tcattaagag catgtttaat ttctgttcta gcctcacctg cacgaagtat tatttgcatt
    16621 gctacttcta ctaattcttc atctattata tgttcttcaa ttgtcatatt tataatttcc
    16681 tttcaatttc taaatttaaa cctcttgact ctctaatttt atctcttctt tctcataagc
    16741 tttaaaaaat ggataccata tcataaatag caatactata tttattgcta aaacaataag
    16801 acctctaaca tcttgattta ccataaaagt agatatacca aaaggaacat accataaatt
    16861 aaacacctta cttggaattg taacaaatcc catactcatt gataaataag tagttattga
    16921 tattactact gttcctatcc acataggtaa cattaaaaat gggttccatg ccactggagc
    16981 tccaaatatt actggttcat ttatattcat tatagaaggg acaatacatg ctcttcctat
    17041 tgcttttatt cttccaactc tggataaaca catcattata actaatggta atgtagcacc
    17101 caaccctcct aaagttatcc agcctgtttg aataacttcg taaacatata tattagttgc
    17161 tactcctcct gatgctactg ctgctgcatt agctgcaatt gcaccaagtt gaattgaata
    17221 agttatagga gatagtaccc acccacttat tcctaaagaa tatataaata ctactaagaa
    17281 gtttaagagt acaaatccag gtaatgtctg tgctatatct gatactggtt taaatactct
    17341 aactattatt tcaaacatgt caaagttgag tccataaatt aatccccagc ctacaaacat
    17401 tatcactgtt ataggtaaca tattatcaaa ccacttgctt acaaaatccg gaagtgctga
    17461 atcttctccg aaaaatgaaa aagaaccaaa taaagacatt attaatccag agaataatcc
    17521 tgcaactagc gatacaagca ttccttctcc tccaaacctg ttgaagttaa atatagctcc
    17581 ttcattcgta atctctggac gaatcatcat taacattaat ccagcacctg caagaccagc
    17641 tactacactc atactttcaa atttcttatt cttcattgtt tccataggca ataaaaatgc
    17701 tataaataat cctaaaagtc caaatgtata ttgatttatt ggacttaaat caggtaaaaa
    17761 gcttatgtaa ttcttcaaga cagaaactat agttactaat gagcctacaa ataccattgg
    17821 caaaactttc attatagttg attgaactga ttgtacccat atattcttag tcatattatt
    17881 tacaacagga gtaaattttt cacttaaaaa atctataaat ttattcataa acactctccc
    17941 cataaatatt ttatttgttt gatattagtt ctagtgcttc ttccaaagcc tttgcaccat
    18001 ctagtcttcc atatgcagtt ttagatatac atgctactgg aacgttatat tcattagcct
    18061 tttgttgaat ttcttccttc atataatcta aatgtggtcc tagtaataaa caatctattt
    18121 ctgataaata cttatctatc tctgactcac ttctcgcttt tatatctgct tctatacctt
    18181 ttttctttgc actctttctc atactagttg ccataaaacc tgtacttgct cctgaaccac
    18241 acgccaaaag cactcttata tttttcataa taatacctcc aatattttta tccattaatt
    18301 tatttcttaa tgaacttttt attttattat gtttatattt tacttcccaa ataagttcat
    18361 ttcacataaa tatatttcct aataatagga aatcatttac tcttaattag acctattaat
    18421 atagtataat gtatcttata atacgtacat tcaattatat aattatcata tcatacagat
    18481 tttattttaa aactatgcga gggaatctaa tgaaaacatc taaaaaagag ctatttttaa
    18541 atttcataaa aacgcaacat gattggatag attcatccac attagctaat taccttaatg
    18601 tatcaacacg ttcgattaga aaatatgtaa acgagattaa ttctaatggt gcatttatat
    18661 tatcttctaa aaagggctat aaagtaaatc tcaatactaa ttgtcaaact aagattgata
    18721 gttcagagaa tatatcacct gataacagat taaatttaat tttaaaagaa cttatagtta
    18781 atagtaatgg aattaatata tttgatttaa gtgaagaatt atttgttagc ccagctacta
    18841 ttgaagggga tatagtcaaa gctaataaat ttataggaag ttataattta aagataaagc
    18901 aatcgaaatt tttattgaaa ctagttggaa atgagtctgc aaagcgcaag ttaatgagct
    18961 ctataatatt taaagaaacg ggaagtgatt ttttaagctt atttgatgta caaaaaatat
    19021 atcaagagta taatttgact aaactaaaag aaaatataat atatatatta aaaaaataca
    19081 atttatttat aaatgagtat gctataaaca acatacttct tcatttaatg ataactatag
    19141 acagaataaa aaagaataat tacatagact ctgttgaagt agtaaactac atagacaata
    19201 acgtagatat aaatattgca aaagacatat caaatttttt agaatctgaa tacaatataa
    19261 ctttaactag tgcggaatta tactatttag tatttcagtt aacaaacaaa actacagtct
    19321 taaactacaa tcaaatggat actaaatcat tgtcaaatta tatagatgaa cactttgtta
    19381 aattaactaa aaaaataatt aaaaatgtat atgacttata tcttatagat ttatctgatg
    19441 aggaatttgt tgtaaaattt actcttcatg taaaaaattt aatcagtaga gccaaaaata
    19501 atcaagtttt aagaaatcaa atacctcaaa agttaaaaga ttcctaccct ttgatatatg
    19561 atatatctgt ttatatttgc aatcagattc aaactttaga aaatgtagat atagacgaag
    19621 atgaaatatc ttatatatct cttcatgtag gttcattctt tgatagacaa aaattattag
    19681 aagataaagt tctatgtgca ttaattactc caaattacta tgatttacaa ttcaaaatag
    19741 taagagacct tgagaagaga tttaatgaat ccatagaaat tattcagatt ttttcagata
    19801 ctcataatct tgattttgac aataaggtag acatggttat tactacttta ccaattaacg
    19861 atagatgccc tatacctttt gtttatgtaa atccttacct aaatagaaag gattatgata
    19921 atattcaaag taaatttatt cagattaaag atagaaaaaa tatacttact gtacaaaatc
    19981 atttagaaat gtactttagt gaatctttgt ttatgaaaaa tatatattta gatagtgcta
    20041 aagattatat aagatttatg ggaaatattc tgtacgaaaa taaatatgtc aaacccaatt
    20101 atatagatga tgttcttata agagaaaaaa tgtcttcaac tgcatttaat aacaatgtag
    20161 caattcctca tagcatgaaa atggatgctt taaaaaccgg agtatgttta attgttaacg
    20221 ataaaccagt taaatggggt gaggaaaaag ttcaaattat agctatgata cctataaatg
    20281 aaaaagaaaa agaaaaattc aattacatat ttgaaagctt tattgaaata ttatctgaat
    20341 ggaataatgt taaagaactt actaaggcag ataactatag tagttttatg aatagaatag
    20401 cttatcttat tcaaaatatc taaatccatt aatttatttc caatatatgt ttcttaagtt
    20461 attaacataa aatagataag ctgtcacact aaaaagtcta aattaatatg tgacagcttt
    20521 ttacctaata taccttgttt taatactaca tattttttga ctgttgtata ctagttataa
    20581 gattgaatat ttgtgttgtt gtagctgtca tattctcact agcagttgct ttatccattg
    20641 cttcttctat tgtcatagct ctatttacta tagaaaaata agcatctata ccctcttcat
    20701 tacatttaac tgcatcttct gttgtggctc cagccaaacc tattactttt accccatatt
    20761 tttttgccag ttttgcaact cctattggag ccttgcccat agcagtttga ttgtccaatc
    20821 taccttcacc agttatgaca aaatctgcat cttttaactc ttcctctaat ttaatttctt
    20881 ctaatattat ttttattcca gattctaatt ttgaatctaa aaatgctaaa aaagcaaaac
    20941 ctaatcctcc tgctgctcct gctccttcta cattagctac atctttactt aagtaatttt
    21001 taactacttt ggaaaaattt ctaagcccat tatctaactt ctcaattatt tctgatgttg
    21061 cacctttttg agacccgtat atgtatgcag ctccattttt cccaaacagc ggattattta
    21121 catcacaggc tattttaaac ttacattcat ctaattcctt taatctattt tctattttta
    21181 tatgtcttat ttcatttaaa acttttccac ctaatcctac taacttgtta tttttatcat
    21241 aaaactcaaa ccctaaggct tgaagcatac ctatcccagc atcattagta gcacttccac
    21301 ctatcccaac tataaaattt ctatagcctt tatttattgc ttcctttatt atttccccaa
    21361 caccataagt tgtagtgtat aaaggatttc taagttcagt cggaaccaat gttaatcctg
    21421 atgcttgagc tatttctatt attgctgtac ttgtattttt taataatcca tatgttgcat
    21481 ccactttttt agctattggt ccagtaactt ctatagtctc ttttttacca ttcatccctt
    21541 gaactaaagc atctactgtc ccttcaccac catcagcaag tggcaatatc tttacttgcg
    21601 catcttcttt aaccttcaat atcccctttt ttattgcatt tcctgcctct attgaagata
    21661 agcttccttt caaagaatct atagaaatta atattttcat acttatctac tcctttttat
    21721 tactccttat tttaaacata taattttata agcttaattt atcttttatt ttatactcta
    21781 catctctctt tgtatacaac ttataccatt aatattcttt tatcttataa aaagggagtg
    21841 tctcaaaata aatttcttag ttcatgagac actcttttgt ataaaatcaa atatatgttt
    21901 attatttcaa caatgataag gaggattatc tctattttaa gataattcct ttttagcact
    21961 atctaatcat ttttagcact atttaaccag tatagtttta acttggttca ttaagcactc
    22021 tacatttaca gagttattat ctattgatat agattctaat tctttttcat ttaggttaat
    22081 ccatttagct gttttaatat ttttgttaaa tactatgtta gctgattttg tttcttcttt
    22141 agaattataa aatctaacta ctaatgcctc ttctttttca gcctttttta caacacttaa
    22201 cacaaggtcc ttattagttt ccttaagtaa actatatgaa taatctactt ctatttctga
    22261 ttgattcaat ttcatagcat tatatggcat tttattataa gtttgtattt ttgttaaata
    22321 ctctttagcc atatttgcaa catttgcttc tagtgtagat ttttcatgag taactattgc
    22381 taagtctatt tttatatctc ctatcatttg agaatctgga gtaggcaact ttattcctga
    22441 tggacgacct gggcgtcttg ccatttcttc tttacctagg aatcctacac ttctaaataa
    22501 tgttatagct attgtgtcaa atttttcccc tactatttca aactctcttg tactgtttgt
    22561 aagaacagct actccatgct ctttgtcaga caatccaacg aatgttagca ttggatatat
    22621 agaatctggt ctttcactcc agtcttcctg ttgccaaact tccatagcat catcatacac
    22681 acttctcttt atatgtccaa actgattatc agacacagaa aactttgaag ctatatttgt
    22741 tggtatataa gttctaaccc tacaatctct tgcttggtta tttatgttga atactacatc
    22801 tattatagct ttatcatttg gaacatttat aactatatca aagtcaataa atgaatctgc
    22861 tctattattt tttctagctt gtatatcttt tggtactttt aatctataat ttatctttat
    22921 ctcattatta aatctatttt tctttatagt ggaatttgct actatattat cgttaaatat
    22981 taatttctca tttggaagtg gtgaaaaatc atactcatct ccttcatctc ctccattttc
    23041 catcaataat acattttcaa aagttttatt taatttttta tcaaatatct ttaatgtccc
    23101 gttttcatta acttgaatag tataaaagtc attatctacc atgtcacata cttcataagc
    23161 tttttctaac atatgttcaa cttcttttat catatacgcc ttatacccca ttgcaggtat
    23221 tttatcttta aagtttattg tatataccat aaatgaatca tagtttccat aatgaacaat
    23281 ttgacggtct attaatcctg catctattac ttctttatgt accacttcaa aatccaactt
    23341 attattatct ttatcaaata tttcaaatgc tttcatttta gttataactt gggcgctcac
    23401 tatttcattc ctatcgtaag gcattaagtt aaatgctatt aacttatcta aagccatttc
    23461 acaactcatt gcatcaacta ttcttctttt gtaaaatgtt attaattggt ctactttttc
    23521 ctcagctaag aaaaatctat tcattatttc ctggtgaact ttatctgaac aacaacatcc
    23581 aatgctgtca tgagcatgat ttttcattat ttctttccaa attaactcta ttaatccatg
    23641 atgatattca aatcctagag aatatgcaat agaagctagt ggctccaata tgttagttat
    23701 ctttgattct attctagcat ttgctgattt tatatccatt cttgttgaga atatacttct
    23761 atgaaccctc atatatttcc catctaaaaa ttcccctttt attgtatcta tatcctcatt
    23821 tttttctagc tctttaaata tgttttcata tctacttaag aaaaattttc tgtctggata
    23881 acattctttt aatttatata taacttcaaa tatattcttt tgaattggca tttggtcatg
    23941 tccatttgga agtattatat ggtcagtagt tgcaccctta tcaagaaccg gtaaatattt
    24001 atccattctt gttcttagct catcttcatt cattggtaga tattttccta ttgcataacc
    24061 taatggaagt atttgaacca aaacagaaga cccatcatca ctcttccaat taaactctgt
    24121 tttattagtt ccttttcttt cagaacatcc tctccaaaac attgaatatt ttatatcaaa
    24181 cccatttagt atttgtggca tttgagcact ttgaccaaat gaatctggta aatatcctat
    24241 tttcatatat tctccaaatt catcacaatc ttttattcca tatagtaaat ttctaagtat
    24301 ggattctcca ccaactacca tttcatcagt ttgagtatac caaggaccta ttattagcct
    24361 tccttcttga actaattttt ttattctctc cttacattca ggcttaactg ctaaataatc
    24421 ttctaatata gcagtttgac catccatcac ataatatgga tagttatgat tattttctaa
    24481 catatccatt atttcttcca tattatttac aagtaatatt ctagattcct cagttgagaa
    24541 gtaccattct ctatcccaat gcatatgagg aactatgtgt acattttttt tcattgctta
    24601 tcctccaaaa cttatctgat aatattttct gtctaattaa aaatccaata aaaatactac
    24661 tgagtttaca aaatttttta atatttattt tatcactata attaaacctt tgatttaaat
    24721 atctaataac gtatctttta atgtatcttt ctttgatttt tttgtttcct agttattatt
    24781 aataatattg ctgatattat agctccaagt actgccgcaa agaaccatat tgatgctata
    24841 ataaatcctg acatacttcc ctctaaaagc gccaatgaga atatccccgc tccaggcact
    24901 tgaagaccta tattgaagaa tgctactata gacccagtta tggctgaacc agctaccaat
    24961 gatggtataa cacggattgg gtcctctatc ataaatggta ttgcaccttc agttattcca
    25021 gccaatccta atatccaagt ttgctttcct atttcctttt ctgattctga aaataaatct
    25081 ctgcctaaaa cagtagctat tgtaactcca aaagctgaaa ccatcttaac tgatgcaaat
    25141 attgcataag gtataaaatt tccacttgct attgctccta ttgcaaatgt ataggctgct
    25201 ttatttattg gtccacctaa gtcaaatgaa accatagctc ctattattgc acctagtagt
    25261 attgcattta ctccagtcat cccatttaac caatttataa gaccattgtt aagtgctgct
    25321 acaggctttc ctactacaaa taacatcaat aaacctacta ccaaagaacc aactactgga
    25381 tatacccaaa aacttacgaa tccagctaat gtgcctttgg ctggtatatt cttctttaaa
    25441 aattttacta tataaccagc aagtaaacct cctaacatac ctccaagaaa tccactacct
    25501 attaaatcag ctgctagacc tgctgcaaat cctggtccaa gacctggttt ttcagctagt
    25561 gagtaagaca tatatgctga taatactgga accatcaatg tacctaacat acctccagaa
    25621 agctttctta attgccataa ccaagaacct tcttgattgt atagttcttg tagaccaaat
    25681 gcttgagatg ctaatactgc aaatgcaagt atcatacctc ctgccacaat aagtggtatt
    25741 atatgagata ctcctgtaag tattgctgat tttacatctt ttttccatga tgatttttca
    25801 caatatgata tatcagatga tttttctata taatcacctt taccttgttt ttgtgctact
    25861 tctaaagctt cgtatattat ttcttttgct ctttttagtg gagctgctac tgacgttttt
    25921 atgtttggca aatgttcaaa tctttctgta tttttaggtg ctacatctgt tgcatatatt
    25981 attgcatctg ccctttttat tatatctggc gtatgagggt ccaccatacc attagctcct
    26041 tgcttttcta catacacatc cacacctatt tctttaccag ctttaattaa tgcttctgct
    26101 gacatatatg tatgtgctat accggcaggg catgctgtta ttgctaatat aatttttttt
    26161 gattcatttt gaatcctaat ctcttctttt atttgttctt tcttttcttt tatatctaag
    26221 ttattaaata actctttctt agtttttgaa tttaacaaca tattcatata atcttcattt
    26281 gacagtctag taactaactc agaaagtatt tgtatgtgtg tatttccacc ttcactttct
    26341 ggtactgcta ttaaaaatat tagctctact ttattatttg ggtctacgct ttcccaattt
    26401 tctattggtg tctctaaggt catagctgca aatgcagctt ctttaactga cttagatttt
    26461 ccatgaggaa ttgcaagccc tctttcaaaa cctgttggag ataaagtttc tctgtacatt
    26521 acagcttcta aatattcctc ataagatgtt aacttgcctt catcatatag ctttcttgct
    26581 aagaacttta ttgcgtcttc ctttgttttt attttttgtt ttaaaaaaac aaggttttca
    26641 tttgtgattt tcttaaattc caaaattacg ccctccttaa atcatttaat gctatgaata
    26701 tagcataaaa taaattatga aaatgttttt ttgaaattat acacacaatt tcttcttaaa
    26761 aaataaagat ttgtgtattt atacacaaat ctctatctta accaatattg ctctattatt
    26821 gctctcataa tcatcataat tggaacctta tctgatatat tgtacccatt tacttgttta
    26881 attgttgaat agcaatataa acttatatct gcaatttgtt gcaattgatt ttcacatata
    26941 tgtgttaaag ctactacctt acaaccctta ttaactgctg tctttgctgc ttctataact
    27001 tctttcgttt ctccacttaa actaagtgca aaaaacatat cttgttcacc taattttcct
    27061 atatcatgaa gtacttcatg tctttgacta tagtaagtta tattatcatg cccacctcta
    27121 atttctttta ctaatatctc acataaagga atgttttgac ctattccata aactataata
    27181 ctcctagatt tttgaattat atctgctact ttatttattt tttcataatc tattagctct
    27241 aatgtctttt ttatatctga ttcaaaacca ttttttggct ctttatcctc attttttaac
    27301 tcttctttta aattattttt tagttgagag aaaccatcat actccatttt tttacatagc
    27361 ctaactatgg tatttgggac agtaaaaaat ttatctgcta aagattgtat tgataatttt
    27421 actatttctt ctttgttttt tctcatatat tcaattatca tgtcttctgt atctgtaaat
    27481 ttatactcat atttttttgc tctttcataa aattccatag tttctcctcc atctatttta
    27541 ttattcataa gtaaaattca aaaatattag ctaaaatttt cgttaatata attataattt
    27601 aatattttta atttattaat gcatactact ctttttatta tacataaaaa atgtgtactt
    27661 cctatattga tagagatgat tcaagttttt attttgatac atctctatca atattttttt
    27721 agaaaataca catttttttc aaatacttat ttttaattaa aactttaggt aatctattct
    27781 ttatatataa ttattagact tgaaaatatc tttaaaatca accaattcaa tattatttct
    27841 ttttaaccac attttaactt ccttatcaca tagcattgca tgttcatata ctctctcttg
    27901 aataagagaa gatgatttta taagactata atctataaat cctggatgag taaccatcaa
    27961 tgttatatct ttatctaaat ttatatattt ttcttcaaat aatttaacaa aagatttatt
    28021 taacttgtaa aaatcattct gaggatattt acttggcatg ctaatacatg aatccaaata
    28081 atagggacaa cttggtatac cgtattcttt tgcagcatca cacattgctg atattgatac
    28141 atcttcttct ataccatgta tatcaatata ctcaggcaat ttactcatat acaacttgta
    28201 agtttcaatt tgagcacaaa cttcttcata tacttcttca taattaaaaa cctctttacc
    28261 tgtcagtagt tgcgttcttc ttattttgct agaaacaaat tttccacctt catctaacaa
    28321 attctttatc ttaatatgtt ctttgcaaca aggttctcct attactaaat taacatgtaa
    28381 accaaaacat acatcatcat tttttaccat ttctatagct tgtcttgaaa aaggcatatt
    28441 caccatgatt ccagcacttg taacaatacc gttcttataa gaagatatta tccctagtgt
    28501 aacaccttct gaatatccta tatcatctgc cctaactact aatttcttca ttttatgcat
    28561 ctgcctgctt tctcaactca tcaagttgca tttccatctc atcctttact ttaacaacat
    28621 ccgttcctat gatgatttga actgatgtat tactcaattt aattacccct ttggctcctg
    28681 tttttttaat attatcatca ttcaaaatag tggcatcctt cacttctaaa cgtaatctcg
    28741 tcatacaatt attaagagtc actatatttt cataaccacc acaattttta attatctgtt
    28801 tagccatata tgaaaaatta ccatgagaaa gttttaaatt cttttctttt tcactaactt
    28861 tatcacctat ctctatctca acttcacgtc ctggtgtttg cacattatct tttattatta
    28921 tatatttaaa tgttacataa tataatacaa agaatacaac tcctacaggt ataactaacc
    28981 acgctttatc tccataacta aaacttaaga catagtcaat tatacttgct ccattaaatg
    29041 aacccaagcg agcacccaat aaatatagta caactccacc tagaccagaa taaaaagaat
    29101 gaattatata taactttgga gatgcaaata aaaacgaaaa ttctattggt tcagttactg
    29161 ttgccataaa agaagtcaat gctccagcgg tcattgcacc ttttacttct tgcttctttt
    29221 tatcttgtgc tgttttatat atagcataag aaatagcagg tattgaaaac atcataatta
    29281 tgtaaaatcc tgtccagaag aatcctgcat ttgggtctcc tgccataaat ctaggaattt
    29341 ctcctcttat aacttctcct gttgatgttg tatattcacc catttcaaat aatatgtaag
    29401 catttaaaac ttgatgtaac ccagttggta ttagtgcacg gttacagaat aaaaacaaga
    29461 atattcctat tgcaccagct cctcccaacc aaaaaccaaa ttgattcatt gcagcttgtg
    29521 gatatatcca taataaacta aatatatatg ataaaattaa agcaaccact ggtgatagtg
    29581 taattacaca tttttctcct gcaaagaagc ttaaaaatgc tggaacttta actttatatg
    29641 ttttattgta agtccatgct atacaagctc caacaattat cccaccaaat acacctgcat
    29701 ttaatgtatc attcaataac tctaaatttg acttaaatat catatatgca actacggctg
    29761 caaccattac acttgtcttg tctttagact ttgagtaggc actagctgct cctaataaaa
    29821 ataagtagtc cacatttgaa aatactatat tagatatatt agatatagct ggcacattga
    29881 gcatatttgg accaaataat aatcctaata tacctgctgc tggcattaca gctattggca
    29941 ccagcatagt ctttcctaaa agtgacagtt gttgcatcat tttgtttaag aatctcttca
    30001 tatccaatcc cctttctatt tcggtttaat tttttgtgaa aacgatttat tcacatttta
    30061 tattttaaat attatatttg tttaaacaaa ttgtcaatat tttttattca ttttattttt
    30121 atcatttagt ttaacatata aatcctttta agtgtattgc tatatctagt ctattttttt
    30181 aattcatttt ttttatttta aaaatatgtt taaccatgta tttgaaacaa cccacaaaat
    30241 aattctattt tgtccttttt atggttgata tgattctatt ttcactatac atgtctaaac
    30301 acatttgata taatttgttc atatcagttc aataatatta ctaaagacct ttaaggaggg
    30361 ataggggtat atggctaaaa ataaattttc agagatatat gaagtattga aagaggaaat
    30421 acttgatggt aaatatacaa gtaatatgat gctgcctact gaacttcaac ttattgaaag
    30481 gttttcatgt agcagaaaca ctgtacgtag agcaatttct caattaaaca cagaaggata
    30541 tgttcaaagt ataaaaggca agggtgttgt agtgttagaa aatagttgtt ctaatgattt
    30601 ttttctaaat atgcacaatt tcaaaggtgt agaatcgatt gtagaagata aaaaggttaa
    30661 tactgctact agtgttctcc acttcagcaa aatcttaatt gataataagt tatctaaaaa
    30721 gacaggtttt aaagttggta gtgaagtata ttaccttcac agactaagat atattgataa
    30781 catacctaaa attctcgaca ttaactattt tctatgtagt atagttaaag accttgatgt
    30841 ttctatagca caaggctcta tttataaata tatagaagag tgtctaggta caaaaattgt
    30901 ttcatcaaga aagatattta aaattgaaaa agctacagaa cttgagttaa aaacgctacc
    30961 tctaaatgat tataattgtg ttggtgttat taaaaatagt gtatatactg atgatggaaa
    31021 gctttttgaa tatactgaat caaagcatac acctgaaaca tttgtattca tggatgttac
    31081 tcaaagatat taaaaaaatt atgtttaatt aaacataatt tttttacgtt tagacacttt
    31141 atcccatact aacctcacta ttctatatac aattatatgt ttatacaaat aattatttat
    31201 tgtatatttt agctttaata ttagtttaac gcttgacaat ttgtttaaac atattataat
    31261 taacttataa attacaggag gtatcaagta tgaaaaattg gtggaaaaaa gcaactgtct
    31321 atcaaatata tccaaaaagt ttcaaagata gcaacaatga tggtataggt gatataaatg
    31381 gtattataga aaaattagat tatctgtatt ctttaggtgt tgatttatta tggttgactc
    31441 ctatgtacgt ttctcctcaa agagataatg gatatgatat agaagattat tataatatag
    31501 acccaaaata tgggacaatg agtgactttg aaaaactctt aaaagaagct cataaaagag
    31561 acataaaaat aatgatggat atggtcctaa accatacatc tactgaacat aaatggttta
    31621 aagaatctaa aaaaagcaaa gacaatccat atcgtgatta ttatttctgg aaagatgcta
    31681 aacctgatgg ttctgttcct aataactgga tatccagatt tagtggaact gcttggaaat
    31741 atgatgaaac cactaaccaa tattatttac atttatttga agaaactcaa gctgatttaa
    31801 attgggaaaa tgaaaaggtt cgtgaagaat gttacaaaat actagaattt tgggctgata
    31861 aaggtattga tggttttcgt ttagatgtag tcaacctact ctccaaaaca cctggattac
    31921 ctgatgaccc tataacagga ccaaaaggag atggaagaac tcactacgct gatggtcctc
    31981 gtatacacga atatttacat aatatgaatc aaaaagtttt caaacctaaa aatatagtta
    32041 ctgtaggtga aatgtcatct actacacctg aagagtgtat caactatact agagaaaatc
    32101 gagaagaact atctatggtt tttagcttcc atcatatgaa aacagattat aaaggaggcg
    32161 caaaatggac taatgaaatg ttctctctgg ataaattaaa aaaagcacaa tctgactttc
    32221 aatataaaat gtatgaagga aaaggatgga atgctctatt ttactctaat cacgaccaac
    32281 ctagagcttt atctagattt ggagatgata gattttttcg tcaagaaagt gctaagatgt
    32341 tagccataac attatttggt ctacagggta ctacatacat atatcaaggt gaagaaatag
    32401 gtatgacaaa tgcctacttt actaaaatag atgaatatga tgatgctgaa agtaagaatg
    32461 catactatca tatgattaaa tctggtattg atgaaaagga ggcattgctt atacttcaac
    32521 aaaaatccag agataatgcc cgtactccta tgcaatggga taatactatt tataaggggt
    32581 tttctaatca taaaccgtgg ttaaaagtta ataatgacaa tattagtgta gaaaatgagc
    32641 ttaatgattc tcgttcagta ttttatactt atcaaagatt aatcaaatat cgtaaacaat
    32701 atgatatttt tacagaaggt acttatagat tgttagatga aaatcataaa aatctatttg
    32761 tttatgaaag agagtataaa aaccaaaatc ttcttgttat aagtaatttt actcatgata
    32821 atgtagtata taaattacct gaaacttatt tgaataaatt aaactacaca atacttgaaa
    32881 ccaattacac tagagatata attgaaaata aaatggaaat aaaaccttac gaaagtataa
    32941 tgatatatta taaataaagt tatttatgta aaaatcggta taacaccctg gtgttatacc
    33001 aattttacta aattcaaagt aatatttata gaatatctat agattacaat aattatttga
    33061 cttctacatc attatatttc tttgaatata ctttagtcat atagaaatat ataggaacta
    33121 caattatcat cccacctaat ccccaaacta tcttcatagg ttcatcaata cctgctttta
    33181 tcagtaataa tatacttata ataactgcaa atattggtat aattgctcct ccaggtattc
    33241 taaaatttac atctttacca gcatacttct ttctcaatat tagtacagct aaacatgtag
    33301 gaatatattg aataaatcta gatacaacac ttatttgaga taatgttgtg aatgaacccg
    33361 accatgctat tagtaaagtt cctatgacag atactataat tgctatataa ggagctccat
    33421 ttttattagt ttttcttata gacttaggca ttaatccttg ttcaaccaaa gcagcagcag
    33481 accttggtgt aaatatggat gatgcaatat ttatacctcc aacagatact atagttccta
    33541 ttgtaattat atacccacct ataggaccta acatcttaat ggcagcatct gcaacaggag
    33601 tagctgaccc tgctaagcca ttacccaaca cccctatact tattcccagt ataagcatat
    33661 atattactga tactacaaac attaccatta ccaatgcttt aggtacattt ttcttagggt
    33721 tctccatatc ttttgctgca acagctaaag attcaaatcc tgtaaaagca taaaataaag
    33781 ttatagatac tgctacaaaa gctgatgacg cactttgacc tttttgtatt attacaaatg
    33841 gggtaaaatt agaacccttt ataaagaata atcctacagc tataaataaa actattggta
    33901 ataattttcc tatagtcatg atattattta agtgcttaga ttgtttcatc cccataaaat
    33961 ttataatccc aagtcctaca actaatatac caattattat atttttagta agtggttctg
    34021 ctgcttgtgg ccatacagat gatagtgtta cagcaaagaa atttgccata gtagcccaag
    34081 caatcatact aactatccac ttcataaatc caacttcaaa tccaacaaaa tctccaaatg
    34141 cctcttttgc ataaagatag ggccctccat cagtatcaaa ccaactacct gcttcagcaa
    34201 aacataaagc aatagaaaca gctaaaagca tacaaaaaac taatactaat atacttgctg
    34261 gacctatatc tgtataagca tcaccaggta acccgaatat acctgaccct attattgcat
    34321 tgattcctaa tagtacaata ctccaaaaac ctaacttaat gcctttttcc attgttattc
    34381 cccccaaagc caacttaata tatttgtatt ttttatttag atgcttcttt aactattgcc
    34441 ataaatattt ttttcatatt atcataatcc ctagtcatca tttctggatg ccattgtatc
    34501 cctattacaa actcacttcc ttccttttct atagcttcaa ttaaaccgtc tttagatgta
    34561 gctgaaacaa tatatcccgg tgctactttg ttgacaacta agtgatgaaa actattaact
    34621 aaaacttctt tttctcctaa tatttcatat aattttgtcc cctcctttat ctgtactgta
    34681 tgtgtaggta cattagatag atgttgctga ttgtgtttaa tataagcacc ttcaattaaa
    34741 gataaatctt gatacaaact acctccttca gttacattta taatttgttc tcctctacat
    34801 atacctaaaa ctggtttttt catatctaaa gcatgtttta ataatttcat atcaaaacta
    34861 tctctctttg gtgatattgc ccccaactta ttatgaggtt cttctttcca tatgagtgga
    34921 tttacatctt gtcccccaga tagtataagc gcatctatat tagatacctg ctcctttata
    34981 atctcatcat catatacaat cggtacaata tatggtatag ctttacacat aactactgat
    35041 tgtatataat cattatttac ataagccctt tcatagccag gaaacattcc tccttcatca
    35101 attagtaaat tacctgatat tcctattatt ggatatttct ttttagccaa tttcttcacc
    35161 ccttaactta tttgtgaaca ataaaaaatg ctacttctct taaagagcaa gtagcatacc
    35221 aagattttat ttatttgaac attataattt attttagaaa tacttttttt gaatacgagt
    35281 aatataaata actttattta cttatatttt attttaatcc tgtttagttt gactttttta
    35341 aattgtttta agtacaacta ataggtaaaa taggcattta gattttcctt tttatcaacc
    35401 tattaaatac tgtttaatat attttttccc cttctattta attcacttcc ctttctacca
    35461 gattcacata ttatcaaatc cagctctttt aacatattaa gtattcccct tacctcacct
    35521 tctgttatat tgtagcctaa ttcatttatt gaattaacaa taccatttct acctatattt
    35581 ttattaagat tattggcatt ttttaaacac tccattacta actttacttt ctctatagat
    35641 gttctatcct ttaatatact tatttcttta ctaaaatctt cagttatatt taataagtta
    35701 tgaggtaaat catttattaa aataacatcc tcagaactca taatgttaat atacatagca
    35761 gtatttttta attctcttat attacctggc cattcatatg cattaataat gtcttgaaca
    35821 tcttgactaa ttgctctctt ccttttcata aaatgcttta acataacttc tatatcttct
    35881 tttcgctctc ttaaaggtgg tatatttatg ggcaatacat ttatcctata gtataaatct
    35941 gctctaaatc tatctccttt tatcatttct aataaatttt tatttgtagc cgcaattact
    36001 ctaacatcta tatctattac attttctcct ccaaccctca ttacttgatt ctcttgtaat
    36061 actctaagta atttgctttg taagtatata ggcatgtctc ctatttcatc caaaaatata
    36121 gttccattgt tagcctgttc aaataatcct tttttacctc cttttaaagc tcctgtaaaa
    36181 gcaccttctt catatccaaa aagttcactt tcaagtaaat tttctggcat agctgcacag
    36241 tttatcgcta taaatggctg actatgtctt ggtgatgcat tatgaataga ctgagccatg
    36301 agttcttttc ccgttccact ttctcccgtt ataagaattg tataatcaga ttcagctatt
    36361 ttcataccta atttcttagt tctttccatc ttcgttgaaa ctgtttttat atcatcaaaa
    36421 gtatatctag caacttgtcc cttttctcta agcttctttg ataggttttg ctccaatttt
    36481 cttatgtaag ttatttcttg aatacaaaaa taatatccag taacccttcc aaaactctct
    36541 atattatact tatttacgtt taagtactta ttattaaatt ttataacttc atcatgtgcc
    36601 ttactcaaac taaaaatctt tttaatattc tcattctcta attcatctat atttttccct
    36661 ataatattac tttgaatatc tagtatatcc cctaaactat tattgcaaat aattatgttt
    36721 cccttatctg ataccattgc catacctaag tttgataaat ttattataga atttaattca
    36781 tctgctttta tagtaagttc tttatattta gtatttattc ctgaatataa actaattatt
    36841 tcatctgtat acttaattag cctttcagta atttctttat catccagttt gagttttgct
    36901 attatttgaa taaacgttga actatcaata tacctatggc ctaaatcaat aacttcttct
    36961 atatattttg gaactaaact tgcctctcct ggcgttatag ctatcttaat atttctatat
    37021 tcttcattgg gattgtatgg tataaaattc agatgattta ttcctatatt gtataaatta
    37081 gatattgttt ccaatacagt atgcttatta tcgtttacta ctaatacatc tattccttct
    37141 ggcaatgaaa ataacttata tatatccttt tgctttatgc tcctatttat agttattatc
    37201 tttgagacat cttctacata tttttttatt tttaatactc tatcatctat cattaccaat
    37261 attatgtcat cttttattaa ttccttctct gttattttat ttaaataata attttttatc
    37321 attactttat ctttaaatat catttctaga tttttattta agaaacattc taatttagac
    37381 aacccatcag ttactactgc tattgttttc atttgattac tccttttaaa atatactcta
    37441 ctcttttata atatacatct gattttcact ttaacagcac aaaaaattgt acatttagta
    37501 tactaacata ttcctttatt agttttatgt ccctaaatat catagttgca atatctatca
    37561 tatttcttaa acttctataa agcaaaaatt gtgccaaatt aataattttc ttaattttat
    37621 tctaaagttc ctcaatagta tttacagact tagcacttat agcttataag tctttatatt
    37681 attaatctat ttttataggt taattgtaca aatcatatat cacttttacc cattaaatgc
    37741 attataaaaa agatggaata acgattcgtc actccacctt ttttgtaata aaaaattgta
    37801 tatataaaca ctatttacta ctcttctaag tacaaacatt atctacatac tataaccttt
    37861 caccattaga ttctattact tgtttatacc agtcaaaact ctttttctta atcctagaca
    37921 agtctccact tccatcatca tacttatcca cgtatataaa cccatatctc ttggccatct
    37981 caccagtaga tgcacttact aaatctatac atccccacgg agtatatcca aataaatcaa
    38041 caccatcttc tacagcttcc ttcatttgct ctatatgtgc ttttaaatac tctatacgat
    38101 agtcatcatt tatcactcca ttagattctt ttttatcata agctcctaaa ccattttcaa
    38161 ctaccatcat aggtattcta tatctatcat aaatttcatt tagtgcatat ctaagccctt
    38221 cagggtctat ttgccatccc cattcactag acttaagata tggatttttc actccaccta
    38281 atatgttgcc acttgtatta tcttctttag aatcactact tacacatgta gacatataat
    38341 aactaaatgt ataaaaatct acacaacctt cttttaaaat ttctaaatct cctttttcca
    38401 tttttatttc aatattattt tctccaaaat atctattcat ataagttgga tattcgccac
    38461 gcacttgtac atctccacag aaataattca ttactctact atctctttga gttttaactg
    38521 catcatctgg attacaagta taaggataca ttgttgcaaa tatttgcata catccaactt
    38581 gataatttga atctatttca tgagctaact taacagcctt agcacttgct acaaattgat
    38641 gatgtaaccc ttgaaaacga agttgaggta tatctacttg gtttataaaa tctgtagtcc
    38701 cttcatttaa tattccttgt gataagtagc ctcccatagg cattgtagct gaatttattt
    38761 cattaaatgt cagccaatat tttacttttc ctttatatct tttaaatatt gtttcacagt
    38821 aattcatata caagtctatt acatctcttg aaacccatcc attgtatttc tctgttattg
    38881 caaatggtat ttcatagtga gatattgtta ttaatggttc tatattatat tttaaacatt
    38941 catctatcac attttcataa aatttaagtc ctgcttcatt tggttctttc tccattccag
    39001 ttggaaatat tctagtccat gctattgaaa atctaaatac cttaaatccc atttctgcaa
    39061 ataaagctat atcttctttg tatctgtgat aaaaatctat tgcttcatga cttggataaa
    39121 atgttccttt ttctataact ctcgttattc ttttacttac tttttgagaa cctccagtac
    39181 acatatctga acaagaagga cctttcccat ctaaattcca tgctccttca cattgatttg
    39241 ctgctgttgc tccaccccat aaaaaatctt ttctaagtgc cataattcta cctccaataa
    39301 tttatttata gtaactttat tttaaaataa aaatacatat atataattct taattaatag
    39361 aactctcttt tacgctaaaa cagttatcaa ttcctgacta ttatttacat gcttttgtgt
    39421 attttctaaa atatccacat aatcagaata attagtaaca actactggtg tttctaagca
    39481 atatccttct tttgaaatac tttctatgtc aaaagttatt aatatctgac cttttttaat
    39541 cttatcccct tgattaacaa aagatttgaa atatttacct tccaactgaa tagtatttaa
    39601 ccctatatgt atcaatagtt cacaaccatt atcagatata attcctattg catgttttgt
    39661 tggaaataaa gtcattacag tcccatcaaa tggtgccact acttttcctt ctttaggtgt
    39721 tattgctaca ccttttccca atatacctgt tgaaaacgct gcatcttgta tctgctccaa
    39781 agcctttact tcacctttta ttgggctata tactacttct cttttgtttt tttctataac
    39841 gccttgccca ttatcatctt tagaaatttc tactatagca tcatctttcc aaaaaaataa
    39901 tgttaaaaca aatcctacta caatagctac tattattgct attatagttg cataaacacc
    39961 tttcatatct ccagttgttg gatttatata attaggtata ccaaatattc caagtccacc
    40021 cattacataa ctttttacat tcattatccc cattacagct cctgccacac caccacctac
    40081 acagctatat atgaatggtg tttttcttgg taaagttatt ccatatattg caggctctgt
    40141 tacaccacat atccctgaaa taactgcagg tatacacagc tcttttatct tcttatcttt
    40201 taatttaaag tacattgctg ctactactgc tgtttgtgca aaagatggta caaatgctcc
    40261 cactaatatt gtgtcaaacc ccatagtcat aaggtttgac atagctattg gtactaatcc
    40321 ccaatgtaga ccaaatatta caagcacttg ccacaataaa ccagttaatg caccacataa
    40381 aattggtgaa aagttaaata aagctaaaaa tccatttcca actatattag aagcataggt
    40441 agctataggc cctataacca taaatcccaa tggtaaagaa actaataaaa ctgccattgg
    40501 tacaaagaaa ttctttacta catctggtat tattttagca aacactttct ccaacttagc
    40561 tgctaataat actattaaaa ttactggtac tactgttgaa gtgtagttca ttgctattac
    40621 tggtaatcct aagaaagtta tatatactgg tgattcaaat attgttccag taaatagatt
    40681 gtataaaggt tgacctgtag atgctagagt atctaattga atacctgaat agcataaaac
    40741 tgcacctata agcattccaa taaatggttt taatttgaat ttcttagcag ctgtatatcc
    40801 aagaattatt ggcatatact ggaacattgc atctccaatt gcatttaaca taatatatgc
    40861 accatcagtt tctccataca aacccaatgc tgtaaatact gcattaaatc cttttatcat
    40921 accagcagca cacagaactc ctaatattgg ttgaaaaact cctgatatta tgtctataaa
    40981 cttattaagt aatccttttg gcgcatctga gtctgaatta tctattgtat ctcctcctaa
    41041 tccagccaaa tcgcacacaa cttcataaac atctggaaca tgattaccaa taactacttg
    41101 gtattgacct gcacttttca ttacagtaac tataccatcc atttttttta gcacatcatc
    41161 atttgctatt gactcatctt ttaatttaaa acgtagcctc gttatacagt gagataaact
    41221 gctaatatta tcctttcctc ctacattcgc aatgatttct tttgatagtt gttcatactt
    41281 tctcattttt tctcctccat tttctttttg aaatatgaag gtttggccaa cttaatgtca
    41341 ccacccaata tatataaacc taattattag gatttatatc tgacttaata cttttactta
    41401 ccactctttc aacgtgaata gttaagtata atttttcctc atctgttaaa tcgtagttgt
    41461 attttttatg tataaattca tccaactttt ctatgaattt ataagcattt ttatatttaa
    41521 cttttattac atttaataaa tcatcttcat tattttcatg taaattatta ctaacaattc
    41581 gttctgcaaa atactttaag tgagtaataa atctatgata ataaactgaa tcttcatcaa
    41641 atactattct gtaatggtat ttaaccatat ttgaaatttc ttgtattatt tgtgtaatct
    41701 catatatagt ttttatactt tgatccattt gtgcattaac tatatgcagt gctataaatc
    41761 cagcttcatc ttctgatagt ctaatttcaa attttttttc tatataatcc aatgcctcta
    41821 accctatttt aaattcactc ttataaaatc tttttatatc ccaaagtaat acatttttta
    41881 cacttactcc ttcacgagct ctttctattg cagtatacat atggtctgtt aaagaaatat
    41941 aaatactatc atttaatctt ttccctagtt tttctttagc atataaaatt atttcatctg
    42001 ataattccat atatcttata ggaatatctg ctattagttc tttaaattta tcagatatat
    42061 ttgggtctga aagtttgaat atcttatcaa ttttatcctt tgatatatgc tctcctgtac
    42121 gttttttata agctaaacct ctccccataa ctactatttc cttatgattt tcatcagttg
    42181 taataattac attattattg agtatttttt caataatcat tgtgatgccc cctttattag
    42241 cattaagtta aaataaatct tttttaatta tttagaatat ttaaaatgta atttattaca
    42301 taaaaaaaaa cctatttatt acacattaaa tagactccca tgagtcctga gtaataaata
    42361 ggtttagctt gtatttaaac aatcactatc cgtttgtatt ttattttaat tattatttat
    42421 tatatgttag caaatttatt ttttagtgtc aactttttct caatatttta attttaaata
    42481 aattatataa attgaatctt caaagtacta tttttatcaa taaacaaaat aaaaaaggta
    42541 ttactttctt taagtaatac ccatttattt ttaatttaag tggtttttct taatctcatc
    42601 aagtagtcca tcttctaaat aaatttctaa tgcacatcct accattgcct ttactgcttt
    42661 gtgaagttta tcataagctt cctcagaatt tgcataattt agaaattctt tttcatgaac
    42721 atataccatt ggacttatat ctaaggctat ttctgtatac atagtagggc aaacttgact
    42781 tacattacct atatctgtag aacctactgg accatcttta ccttctaata tatctgttat
    42841 acctgcctca atcaaattat tctttgttat cttttgaagt actttgttat ttattatgtt
    42901 gtcaaaagaa ttttcaaagt atctataaga aagcttagct cctgtcatta actctgcacc
    42961 ttttgcacag tttataactt ttttagttac ttcatctaaa taacttcttt cagcagctct
    43021 tacgaaaaac ttacaagctg ctttttctgg aactatattt ggagcctctc ctccatttga
    43081 aactatacca tgtattctaa catctgattt tacatgttgt cttagtgcat ttatcccagc
    43141 aaaagtaagt tgtactgcat ctagtgcatt gactcccata tgagggtgac tagctgcatg
    43201 tgctgatacc ccttcaaaac taaactctaa tgaatctatc gccaaagcct tacagtttat
    43261 attattaaaa gcttctatat gcatttgata acttacatca acatcatcaa aagctcctgc
    43321 cttaactaaa tcacattttc ctcctgttgt ttcttcagct ggtgttccta ttagtacaat
    43381 ttttccttta aaattttctt ttaacttact taatactata caagctccta gtgtagatgc
    43441 tgcaatccag ttatgtccac aagcatgagc tggttcttta ttttctccat atccaggaag
    43501 tgcatcatac tcagctaaaa atgctatggt aggtccatca ttatctccta attcagctct
    43561 aaaagcagtc tccatattac aatatggata aactgtatca aatccatatt ccttcatttt
    43621 ttctactaag tattttgatg atatgtattc ttcttcccct aattcaggat tgttaaatat
    43681 aaaattacaa acttcttcaa tttcttttcc aagttcattc tctatctcat aagctctctt
    43741 ctttatactc tctatcataa tttacctcct actagaatcc tagatataat cctattgtca
    43801 aaaatgccat tacaagaatt gttaatacta ccattaaagg aattacgaat ctaacccact
    43861 ttgaccaagg aatattggct actgctagtg ctcccattaa aacacctgat gtaggtgtta
    43921 tcaagttagt tacacctata ccaaattgat aagctgtaac tatcatttgt cttgatacat
    43981 ctactaagtc gccaagtggt gcaagtacag gtattgtaag tgatgcaagc cctgatgctg
    44041 atggtattaa aaatgctatt aatccttcta ttaagaaagt taaatttata aatatagttt
    44101 ttggtaatcc atttaataaa tttgctgcag aatttaaaat tgtatctatt ataaatccat
    44161 tttctgctat tattacgatt ccacgtgcaa atgctataca caatcctgct gtaattaaat
    44221 ctcctgctcc acttataaat gaattaacta tctcatcttg tttaagccct gatgctattc
    44281 ctgatattat acctatagct gtaaatatca ttgcaatttc agttatgtac cattctagtc
    44341 ttaaaactcc atatatcata atcgccatac ctattgtgaa aatggctatt accatggctt
    44401 ctctttttgt aaattccttt atatttgtat cactattaac cattaattct tgatttagtg
    44461 atatatcatg tactaaagat tttttagggt cttttttaac tttattggca tacatcataa
    44521 caaatcctat agatatagcc atatatatta taaacataac aactctaaac tctattccag
    44581 aacctggtac tatttgtgat aatgcttgtg ctattcctac agagaaagga ttcgtagtag
    44641 ctgctccaaa tccagctgtt gctcctataa atactgtggc cactgctgtc aatgaatcat
    44701 atcccatact ggtcataagt ggaataaata tcatataaaa aggtagtgtc tcttcactca
    44761 ttccaaacgt cgtcccacct agtccaaata gaatcattga aatcggtatt attaaaaatt
    44821 gaaacttctt cattttattt acagtatgag ctataacagc ttctatagct cctgttttat
    44881 taactatccc aaaagctcct ccaactatta aaacaaatcc aactacttca gctgcatcta
    44941 taaacccgtt tataggtgct gtaaatacat cagtaattcc ttgtggattt gatggtgctt
    45001 tttcataagt tccagctaca acaactgacc ttccgtctat atctactctt tcaaaatttc
    45061 cacttggcac tatccaagta agaattgcca ttaacactat tagcaaaaat attattgtaa
    45121 gtgtctgtgg catagtaaac tttttctttt ttaaactgct catttttaat ctccccactt
    45181 tttaaattaa atattttatt attgcatccc tttatcctat tttgtataga tgtaaaaaag
    45241 ctcaacatgg atacatgttg agctaaatgt gctaaataca acatacctgc atcttccctt
    45301 tatcttgttt aagatagctc tccatcaaaa ttcgggataa ttttgatgac agtactgtac
    45361 ctattaaata cagtcccagc actagcttcg gacactagca cttcggcact tactcctttt
    45421 atattgcctc attggtttcc aacctcttca atattaatct tagtaaatgc aacctctacc
    45481 tcaatctgtt tgttagatga ggtaattctt attaattttt attaattcaa agtaattgta
    45541 gcataatatg tcgagattgt aaatattttc agtataataa ttcttacttt atgatttcat
    45601 tccatttttc acaaataatt ttttctccat ataatttaaa tactgtagta tattttaaat
    45661 acacacaaac tttccacaaa ttttaaatta ttataatacc tttataataa aaaataaaag
    45721 ctatgtaaac ttatcaattt acacagcttt atatacatta atctactcca agtacagctt
    45781 taagctttga tgctattgta ataactcgtg gaccaaatat aacttgaact gagtttttct
    45841 ttttaacaac tccacttgca cccaattcat ttacccagaa gccatcttca tagaatccat
    45901 caattgaatt tagctctacc ctaagtctag ttgcacagtt tgtaacattt tttatattat
    45961 tacttcctcc taaaccttct attatagaat gtacaagctt tgtatcttta tcatctactt
    46021 cctcttctac tgcattgttg tatgctgttt taccagcatc tacagattgt ctttctctat
    46081 attctttctt actataaagc ttaacttctt ctgaacttct tcctggtgtt tttatatcaa
    46141 acttcttgat tgcccatgta aatattatat aagtaactat aaaaaatatt ggtatcaaaa
    46201 ataataatgg taacgcatcc actttttgtg gttgtaataa attaggaaac atatttctaa
    46261 atgattctcc cattatagaa acattaaaca tttctgtaaa tacatacgtc aatcctgcca
    46321 gtggtacata tgctacaaaa tacaataatg gtgcaacaaa taggaatgta tactctattg
    46381 gttccgttac ccctaccaac atggctgtta ctattgctgg tatcaatata cttattgctt
    46441 ttttcttatt agcattatca gccaacttat acatagctaa agctgctgct ggtaatattg
    46501 caaaatgtat taataaacga cctgttgtga agttacgagt tatataagat agtgtatctg
    46561 ggtctcccat ttgagctaat cttatattat taacaccctc gtaaattgct ccacctactt
    46621 ccattgttcc acctactctt gtatattcta tagggaatgc aattaagtga tgtataccaa
    46681 atggtaataa tgctttatcc attgctccaa aagtaaatgt tcctaataat ccactagaac
    46741 ctatccaatt accaagttta gataatgcca accctactgg tggccatata aagtacattg
    46801 ctgtacccaa tactattgat gctataaaca tcataatcat tgaaaatctt ggacctgaga
    46861 aaaaagataa tgcactagga agttctatgt caacaaattt gttatgtact gcactagcta
    46921 caaatccagc tattatagct ccaaatatac tacaatcata tgaaaagatt cctaagaaag
    46981 aaccatataa agaacttcga ctatatgctt ctgcctctcc taatccagat gccataaacg
    47041 cttctgctgt aactgtatct gaatttatac catttgctgc taacaatgta cttataactg
    47101 tgtgcattcc tataaataaa acaattcctc ctaatgcacc ccatcctttt tcctttttag
    47161 ataatccaaa ggctatacct gtaacaaaga atattggtaa gtatctcatt accataaatc
    47221 ctaaatcgtt tattacttta aagaatacat ttataaatga cccttctttt attagtccgc
    47281 taagccctaa tgcagaaacg ttttccatat ttgcaaaagc tgaccctaat cctataaata
    47341 ctcctgctac aacaagtaat ataacaggta tcatcattgc tccagaaagt acttgtaatt
    47401 tatccttcat agtattattt cctccatttt tattgaaatg ccaggctata taatgcatat
    47461 atatatactt ctattgcttt gtataatgta tctatactta tattttcatt tgcctgatgt
    47521 gaatttggat tatccccctc tattgtgggt ccaaatgcaa ctccattttt taacactctt
    47581 gcataagatg cagctccttt tgtaaaaagt tcggcttctt ttccaaaacc ttgtttatat
    47641 gcatttgata atttatttat taattcacta tctggtttta catacagtaa ttttaaatcc
    47701 ttatatatat caactattaa attttctttt tttccaattt cattaattct attagttata
    47761 aactcccaac ttataccctt aggatatcta aagtctattt tcatattcaa ttcatttttc
    47821 tctaagaaga tactcattat acatattgta gaaactccca tatctacatc ttctttatac
    47881 atccctaatt tttttccatg attatcatct gtaaagtatg aatttaaaat atcccttatt
    47941 ataacgcctt tatcatttag ttttttaatt ttctctaaat cttttgctaa actaaatgca
    48001 cagttatatg acctgtgtgg atttctcgat aaagcccttt ctcctgtata tcttactaat
    48061 actttacctt catctaattc ttctatctct gtataataag ctaatgattc tactaaatct
    48121 ttcttatcat tagttttaaa aacagcctca attttgtcac atacaaatcc atcttctttg
    48181 tttgacttta tatcaactaa attgtgattt ctaaatttat ctttatctat tttctttctt
    48241 agattgaagt ataaaatacc tttttctcca tttactattg ggaagtttcc atctggagaa
    48301 aatgcaaatt ttggctgttc attgacacta aagtaatgct ccatacattc ccaagtcgtc
    48361 tcttcagctc cacccactat caatctaatc tttctcttcg gcttttcatt aagttctctt
    48421 aagaaaagta atgcatataa aatccctatc aaaggtcctt tgttgtcatt aactcctcta
    48481 ccataaagat agttatcttt ttgacatact ttaaaaggtt tactcttcca aagttcttta
    48541 ttatatattg gcaccacatc tatatgatta agcaccccta ctacttcttc tccttctcca
    48601 tattctatat gaattgcata tccatcaaag tcttttacaa caaaatcttt gtcttttgct
    48661 atttgaataa gcttatcgaa ggcatttctt atttctatcc caaatggttg atttatatct
    48721 gttgtacttt catctctaat tgaaggtatt tcaactaaac ttctaatatc atttatgata
    48781 ttttcttgat tggattttaa ataatcaata attagttctt tgtttactat catcttaatt
    48841 atttatcacc cctttatatt aattttacaa agcatttcag ttcatatcta cattataaat
    48901 tttgaatatt attttttcaa tatttcaaat acttctctaa gttaccataa tgatacggta
    48961 aacaatcaac agattatgac aaaatcatac tatacctatt actaatattt taacttatct
    49021 attacgtctt ttttaatgta agtttattcc aaaaatacat aaaaaaagat gtagccttag
    49081 ctacatcttc ttatatctat tcaacttcca ttattatgct attaatatct acatttccag
    49141 gttctatcct atcttttact gttctcttaa cagaatcagc atcatccatt ataactacag
    49201 gagttattat atttaaatca tttgacgtta taaaatctaa atctacttcc attattaaat
    49261 ctttagcttt taccttatct cctgccttta ccttaacatc aaacggcttt ccttctagtg
    49321 acattgtctc taaacctaca tgtattaata tatttgtatt atcattactc ttaatcatta
    49381 ttgcatgctt tgtatcaaat acattgacta tttcaccatc aacaggagaa tataacttac
    49441 cttcacttgg tattattgct actccatctc ccatcatctt ttgggcaaaa actgcatctg
    49501 gaacatcttc taagttcact aaatttccct taaaagggct atatattttc tcaatcttat
    49561 tttctttttt cttaaatata ttaaacataa cacactctcc acattataaa attttatgat
    49621 aatatttttc ttaaagcttt tgctacacca tcatttacat tagtatcagt tacaaaatct
    49681 gccatagatt ttacttcatc tgtagcattt cccatagcaa taccaagacc tgcaaattta
    49741 atcatggata tatcattttc attatcacct atacaaataa cttctttgct atctatacct
    49801 aatatatcac aaaatttttt aactgcaata ccttttgaag tacctttact cataacttca
    49861 aagtttatct ttccagaact tactacttct aaatcttctt gttttttaaa ttcttccttt
    49921 gcttctctca aagaatctac gttttctgag aaaagtatac attttaaaat ttcattatca
    49981 gctttttcta ataaagtttc acaacttgca ttttctatta tttcaattct gtcttcttct
    50041 ttatttttac tatttattaa tctatatgga tgttcttcac ctatttcatt gttggatata
    50101 actattttac agcctttaaa atgtttgttt aaattatact tttcacctat tgtatagatt
    50161 ttctttagag cttctttgct caaaaccttc ttatactcat atcccagtaa tttaaaatat
    50221 gtcccattag ttgatataat atatatatca tcccctaaaa cacttgtaaa atatttcata
    50281 gcattataag gtcttcccgt aactaaggct accttgatac ccttttccat ggcttctttt
    50341 agtgctttaa tattttcttc tgatacttca aatcctttac ccatcaaagt accatccata
    50401 tcagtacaaa ttaatttata tcgcatttgt atcacatcct agtaagattt ttctaatatt
    50461 tcaacaaggt cttctttctt tccaaaaggt aaaaatccta aaactggaca gcttgttatt
    50521 tgttctaatt gttcctttgt tacattaaac tcagacattt ttgtcttaag ccccactctc
    50581 tttaaaaaac tttctatttc atttcctaaa gttttagctg cttcatcatc ttccactttt
    50641 tctaattctt ggtttagtat tctcgctact ttggcaaatt tctctatatt atccttatag
    50701 aacttttctg caaattttgg gaacactata gccaaagcct caccatgagg aatatgtgta
    50761 actcctccta ttatttcact tagtggatgt ggtgctgctg ctccagcatt tgccagtgaa
    50821 cttcctccta atgtatctgc tactgctaac tttgttctgt attctatatt atttttatct
    50881 tccattactt taggtagata gttaatgact aattccatag atttttcaga cattatctct
    50941 gtaaaatcat tagcatttgg atttatataa ctttcaaatg catgagtaaa agcatcaaat
    51001 gctgttgatg cagtaattct ctctggcaga gttaacataa gctctgggtc aactatacat
    51061 tcttttgaga aacaattttg atgaaatatt gtatttttct cgcttcctct cgatattact
    51121 gctgcttgtg taacttggct tcctgttcca gaagtagttg atacagctat taatggtaat
    51181 tctttttcac ttaccttatc gtatatttta aatggatttg tatatgtact aaacatataa
    51241 tcccaatcta ttttttctaa accaaaagtc aatgctaata cttttgcagt atctatactt
    51301 gaacctcctc caacagctaa aacaacgtct acagattctt ttttagctaa ttcaaaccct
    51361 tcgtttatta attctacagt tgggtttggc tctactctct gaacctccca tgactaaagt
    51421 cacagggttc ctgcttcaca gaattttgca tactaaaata tgtcttacta attctccaca
    51481 ggccatcccc gtagttccta cggttcttac ataagttatt tattttgaat tgttattagt
    51541 cttaatcctt cttttaatat atttatactt gcatttatat ctctatcgtg agtttcatta
    51601 caactaggac aaatccattc tcttatattt aagtccttca cttcctcgtt tttataccca
    51661 catttattgc atatttgtga acttgcaaag aatttaccta cctttacaat ttgtctacca
    51721 tgccaattag ccttgtattc taattgacga ataaattctg accatgatac atctgaaatt
    51781 gagcgagata gtttacgatt tttaatcata ttctttactt gtaaatcttc tatgcaaata
    51841 atatcattct cttttattaa tttagtagat aatttttg
//
LOCUS       DAMJZE010000008        50101 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJZE010000008 DAMJZE010000000
VERSION     DAMJZE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.008"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(87..545)
                     /locus_tag="PC541_000358"
     CDS             complement(87..545)
                     /locus_tag="PC541_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837845.1"
                     /translation="MKKIIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSIVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGEINVYKAGKIVSSQLVG
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(580..882)
                     /locus_tag="PC541_000359"
     CDS             complement(580..882)
                     /locus_tag="PC541_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837846.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(1270..1422)
                     /locus_tag="PC541_000360"
     CDS             complement(1270..1422)
                     /locus_tag="PC541_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837847.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(1604..2761)
                     /locus_tag="PC541_000361"
     CDS             complement(1604..2761)
                     /locus_tag="PC541_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDF2837848.1"
                     /translation="MNQVKDQKDNILMKSNKFIMARYDLQTIENRLFVRLLYEFQKNK
                     NLEITITIEEVRELMKNPNYTTPQAISKIFTKLRTGTIYIKDDDSWGECSFIYHWIYN
                     EKDKTYTVKTSAELLDLIENYIQVGYTPINLQIFFSLRNSYAQRFYDLLRLWTRTKNK
                     ITYTLDVLKELLLLEDKYSKYNDLKRRVIVPAIQELNSTEYFDIDFKEIKKSRKVVAI
                     EFSVKDLDKRKYFDKNVVETVIYEEQEESKETETTIEFYIPDETAFTKGTIRLFKIDF
                     KEFDFKKDIYIQAFDKSLAIALERDNADIINTKNYDFFKSTLSNKISEVIIQEKDDLK
                     FKEELDKYWDDEDVKKEEREIDYSKDPVKLETVRRNLFDQGLVNEEGYYYK"
     gene            complement(3644..4027)
                     /locus_tag="PC541_000362"
     CDS             complement(3644..4027)
                     /locus_tag="PC541_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2837849.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHRNSFESFISAL
                     HDDEEISANKLKSLEKFIEDWEEDK"
     gene            4613..4768
                     /locus_tag="PC541_000363"
     CDS             4613..4768
                     /locus_tag="PC541_000363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837850.1"
                     /translation="MGELKLESKMNCTKNKKKVAISIHQMKKISKKDYVFLTDCIETF
                     YMKIKEV"
     gene            complement(4794..5162)
                     /locus_tag="PC541_000364"
     CDS             complement(4794..5162)
                     /locus_tag="PC541_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2837851.1"
                     /translation="MAEVSKKLKAKEIGKRLNELRTLERLSQRAFGERIFLSQDQISL
                     LEKGKRVLTERSINDICREFEVNEEWLRSGKGEIYKDCLADLEIDDDVKEITNKLYEL
                     EQEDRDAILKMIELLQKKNK"
     gene            5334..5495
                     /locus_tag="PC541_000365"
     CDS             5334..5495
                     /locus_tag="PC541_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837852.1"
                     /translation="MICSNKEKVETALMIDYLKKNEPNEWRTIETIISSTYALSNFKK
                     ENKDIMNQK"
     gene            5527..5742
                     /locus_tag="PC541_000366"
     CDS             5527..5742
                     /locus_tag="PC541_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837853.1"
                     /translation="MKISDFNKNQIGTAVQLQDIRFGTKVDCIITKVEKNEILVMYYE
                     KETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            5771..6502
                     /locus_tag="PC541_000367"
     CDS             5771..6502
                     /locus_tag="PC541_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2837854.1"
                     /translation="MDLLKDARKKEWFWLENDLVDREDLGIYEKMIYIVLARYSDNES
                     CCFPSYKTIALKCGCSKRQAKSVVKILENKGLIKKENRIKSNSNEKESNIYFVLTAKL
                     GGEYDAQQVVNMMHNPSAPHAQQVVHEVHSKKTYNKKTYIKSNTTPQNEPKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYMHDKIVSLENYIVNGKGSRYKSHYRALLTWG
                     NADTSKGILQPVTKAKNPLSGFKEL"
     gene            6531..6716
                     /locus_tag="PC541_000368"
     CDS             6531..6716
                     /locus_tag="PC541_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837855.1"
                     /translation="MNEKEEAVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFEIKSDI"
     gene            6742..6960
                     /locus_tag="PC541_000369"
     CDS             6742..6960
                     /locus_tag="PC541_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837856.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(6979..7191)
                     /locus_tag="PC541_000370"
     CDS             complement(6979..7191)
                     /locus_tag="PC541_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837857.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            7354..7614
                     /locus_tag="PC541_000371"
     CDS             7354..7614
                     /locus_tag="PC541_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837858.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(7607..7795)
                     /locus_tag="PC541_000372"
     CDS             complement(7607..7795)
                     /locus_tag="PC541_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837859.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            7933..8250
                     /locus_tag="PC541_000373"
     CDS             7933..8250
                     /locus_tag="PC541_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837860.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            8272..9603
                     /locus_tag="PC541_000374"
     CDS             8272..9603
                     /locus_tag="PC541_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2837861.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            9978..10364
                     /locus_tag="PC541_000375"
     CDS             9978..10364
                     /locus_tag="PC541_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837862.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            10417..11121
                     /locus_tag="PC541_000376"
     CDS             10417..11121
                     /locus_tag="PC541_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837863.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            11178..11360
                     /locus_tag="PC541_000377"
     CDS             11178..11360
                     /locus_tag="PC541_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837864.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            11357..11734
                     /gene="ssb"
                     /locus_tag="PC541_000378"
     CDS             11357..11734
                     /gene="ssb"
                     /locus_tag="PC541_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2837865.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            11715..12299
                     /locus_tag="PC541_000379"
     CDS             11715..12299
                     /locus_tag="PC541_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2837866.1"
                     /translation="MKIFHFKLDGDYVRSLEERNKLVEENINFVWFIIRRYFKVFISK
                     YPYLQDDTFSEGCIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRAD
                     IDSFEKCIYKNTHGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREK
                     GYTQKEIGKILRTNQMTICRKLGKLKSEINILGK"
     gene            12329..12487
                     /locus_tag="PC541_000380"
     CDS             12329..12487
                     /locus_tag="PC541_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837867.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            12490..12816
                     /locus_tag="PC541_000381"
     CDS             12490..12816
                     /locus_tag="PC541_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837868.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            12816..13352
                     /locus_tag="PC541_000382"
     CDS             12816..13352
                     /locus_tag="PC541_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDF2837869.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            13364..14170
                     /locus_tag="PC541_000383"
     CDS             13364..14170
                     /locus_tag="PC541_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837870.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            14195..14455
                     /locus_tag="PC541_000384"
     CDS             14195..14455
                     /locus_tag="PC541_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837871.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            14479..14964
                     /locus_tag="PC541_000385"
     CDS             14479..14964
                     /locus_tag="PC541_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837872.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            14984..15241
                     /locus_tag="PC541_000386"
     CDS             14984..15241
                     /locus_tag="PC541_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837873.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            15350..15793
                     /locus_tag="PC541_000387"
     CDS             15350..15793
                     /locus_tag="PC541_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDF2837874.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            15936..16481
                     /locus_tag="PC541_000388"
     CDS             15936..16481
                     /locus_tag="PC541_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837875.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            16485..17129
                     /locus_tag="PC541_000389"
     CDS             16485..17129
                     /locus_tag="PC541_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2837876.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            17344..17802
                     /locus_tag="PC541_000390"
     CDS             17344..17802
                     /locus_tag="PC541_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2837877.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            18357..20096
                     /locus_tag="PC541_000391"
     CDS             18357..20096
                     /locus_tag="PC541_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837878.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            20097..20300
                     /locus_tag="PC541_000392"
     CDS             20097..20300
                     /locus_tag="PC541_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837879.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            20313..21590
                     /locus_tag="PC541_000393"
     CDS             20313..21590
                     /locus_tag="PC541_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2837880.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            21568..22329
                     /locus_tag="PC541_000394"
     CDS             21568..22329
                     /locus_tag="PC541_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2837881.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            22388..22714
                     /locus_tag="PC541_000395"
     CDS             22388..22714
                     /locus_tag="PC541_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837882.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            22740..23342
                     /locus_tag="PC541_000396"
     CDS             22740..23342
                     /locus_tag="PC541_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDF2837883.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            23371..24294
                     /locus_tag="PC541_000397"
     CDS             23371..24294
                     /locus_tag="PC541_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837884.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            24308..24580
                     /locus_tag="PC541_000398"
     CDS             24308..24580
                     /locus_tag="PC541_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837885.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            24564..24944
                     /locus_tag="PC541_000399"
     CDS             24564..24944
                     /locus_tag="PC541_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDF2837886.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     TALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            24956..25303
                     /locus_tag="PC541_000400"
     CDS             24956..25303
                     /locus_tag="PC541_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837887.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            25303..25659
                     /locus_tag="PC541_000401"
     CDS             25303..25659
                     /locus_tag="PC541_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2837888.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            25668..26117
                     /locus_tag="PC541_000402"
     CDS             25668..26117
                     /locus_tag="PC541_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837889.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            26118..27173
                     /locus_tag="PC541_000403"
     CDS             26118..27173
                     /locus_tag="PC541_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2837890.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            27189..27629
                     /locus_tag="PC541_000404"
     CDS             27189..27629
                     /locus_tag="PC541_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2837891.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            27644..28060
                     /locus_tag="PC541_000405"
     CDS             27644..28060
                     /locus_tag="PC541_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2837892.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            28087..28236
                     /locus_tag="PC541_000406"
     CDS             28087..28236
                     /locus_tag="PC541_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837893.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            28240..31629
                     /locus_tag="PC541_000407"
     CDS             28240..31629
                     /locus_tag="PC541_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2837894.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            31676..32152
                     /locus_tag="PC541_000408"
     CDS             31676..32152
                     /locus_tag="PC541_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2837895.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            32210..32845
                     /locus_tag="PC541_000409"
     CDS             32210..32845
                     /locus_tag="PC541_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2837896.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            33086..34603
                     /locus_tag="PC541_000410"
     CDS             33086..34603
                     /locus_tag="PC541_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2837897.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            34617..34949
                     /locus_tag="PC541_000411"
     CDS             34617..34949
                     /locus_tag="PC541_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2837898.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            34942..35376
                     /locus_tag="PC541_000412"
     CDS             34942..35376
                     /locus_tag="PC541_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2837899.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            35369..36421
                     /locus_tag="PC541_000413"
     CDS             35369..36421
                     /locus_tag="PC541_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2837900.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            36423..37031
                     /locus_tag="PC541_000414"
     CDS             36423..37031
                     /locus_tag="PC541_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2837901.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            37048..37830
                     /locus_tag="PC541_000415"
     CDS             37048..37830
                     /locus_tag="PC541_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2837902.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            38426..38914
                     /locus_tag="PC541_000416"
                     /pseudo
     CDS             38426..38914
                     /locus_tag="PC541_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39316..39663
                     /locus_tag="PC541_000417"
     CDS             39316..39663
                     /locus_tag="PC541_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837903.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            39675..39845
                     /locus_tag="PC541_000418"
     CDS             39675..39845
                     /locus_tag="PC541_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837904.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            39875..40156
                     /locus_tag="PC541_000419"
                     /pseudo
     CDS             39875..40156
                     /locus_tag="PC541_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40464..40931
                     /locus_tag="PC541_000420"
     CDS             40464..40931
                     /locus_tag="PC541_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2837905.1"
                     /translation="MSKSYFNTKLGGSMNVTIVFLATNIFIKLVILAIAFDTLLGCLR
                     AIKTHQFNSSFGINGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLG
                     ISEFFSILFILYESVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            40935..41765
                     /locus_tag="PC541_000421"
     CDS             40935..41765
                     /locus_tag="PC541_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2837906.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            41837..42943
                     /locus_tag="PC541_000422"
     CDS             41837..42943
                     /locus_tag="PC541_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2837907.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            43081..43416
                     /locus_tag="PC541_000423"
                     /pseudo
     CDS             43081..43416
                     /locus_tag="PC541_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(43427..43942)
                     /locus_tag="PC541_000424"
     CDS             complement(43427..43942)
                     /locus_tag="PC541_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837908.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(44025..44930)
                     /locus_tag="PC541_000425"
     CDS             complement(44025..44930)
                     /locus_tag="PC541_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDF2837909.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(44950..45153)
                     /locus_tag="PC541_000426"
     CDS             complement(44950..45153)
                     /locus_tag="PC541_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837910.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(45277..49419)
                     /locus_tag="PC541_000427"
     CDS             complement(45277..49419)
                     /locus_tag="PC541_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2837911.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT        19622 a         6064 c         8333 g        16082 t
ORIGIN      
        1 atataattaa aaacacactt tttataattt tgcataaaaa aagagtgttt catatatttc
       61 taaacactct tttctacttt ttataattat ctaattgtaa aagaagcttc agctggacta
      121 gagaataata taatacctcc tgtgcacaca ccttttcctt taaatttagc aatatatgaa
      181 ccctttttta gccctttgaa acttactaca ccagttttag tagttccagc taatctaaag
      241 cctacaagtt gagatgatac aattttgcca gccttatata catttatttc tccttcaaat
      301 cctaaaatat ttccaagcgt agatgttact ctccaagata actgccctcc actcattgct
      361 gttaaattag cagtaccacc tggaacgaca atagatttag ttgaaggcga atctgagttt
      421 ggttgtccaa ttaattcaac tgtttgtgag tttccagtac cttcataatc atttccatca
      481 gcaaaagctg taaatggaat cattactgcg caaagcgtta gtaataaagc aattattttt
      541 ttcatttgtt ttccccctat ttttttatat tattctcaat tacagtttcc tttctttacg
      601 tgccttaata atcatattgt aattatgctc agcagtttca aatgctccta ccaaaggagt
      661 tataattaca ctttctatta agcacaattc atcatagttt aaactagctt ctccagcata
      721 gaaattattt aacttatcta aatctattct agttaaaacg gaaactgatt ccattgagaa
      781 tttgtaaaca tcaataagtt tatataatcc acctatatag ttttttgcta cttcatggct
      841 cttaaaaaat tcatcttttc ctgtatgtag gtttacttcc ataattccct ccattgttct
      901 tatattttta accttcaaat aatatttact tcttcttcaa aacagaatat tctatcatgg
      961 tagaatattc tgtttttttc attctatcac gatagaataa tattttcaat aattttttct
     1021 attctatttt atttaaatag aatagaaaaa aagaacactt aatatttttc aagctaatat
     1081 attaagtgtt ctttttatat ttttacaaga ttatataaaa ttattcttta atcaattaaa
     1141 gtgtttttct atttcttaat aataagaaat gatataaaat tgtatcattt taaagatttt
     1201 gtgatataat aaaagcaaga agaactacaa tctatttagg cgtagagtgg agttcataat
     1261 aaacatagtt tattttttga atttaaatat aaatttaaat tcaacctgta agtcactctt
     1321 tgcacgagag tggctttttg cttttttgaa tagtttactg attaagtaaa ctaccacact
     1381 agctgttaaa ctagctaaaa cattaaacaa aaagttatcc atgtaactca cctcccctcg
     1441 aatcgttggg aggataatct tttgtacatg aactccactc tatagattgt atattacgct
     1501 cttcttgcta aatttaatta taccatattt tgtaatatca gttaagagtt tgcactacaa
     1561 taatccttca ttactgctta tcttctttaa ttttctccaa ttcttattta tagtaatatc
     1621 cttcttcatt tactagcccc tgatcaaata aatttctcct aacagtttca agtttgacag
     1681 gatctttgct ataatcaatt tctctttctt cttttttaac atcttcatca tcccaatatt
     1741 tatctaattc ttccttaaac tttaaatcat ctttttcttg aattataact tcacttattt
     1801 tatttgaaag tgttgatttg aaaaaatcat aattctttgt atttataatg tcagcattat
     1861 ctctttcaag tgctatagct aaagatttat caaaagcctg tatataaata tcttttttaa
     1921 aatcaaattc tttaaaatca atcttaaaaa gtcttattgt tcctttagta aatgcagttt
     1981 catctggtat ataaaattct attgtagttt cagtttcttt actttcttct tgttcttcat
     2041 aaattacagt ttccacaaca tttttatcaa aatattttct tttatctaaa tctttaacag
     2101 aaaactcaat agctactact tttctgcttt tcttaatttc cttgaaatct atatcaaaat
     2161 attctgtaga attaagttcc tgtatagcgg gaacaataac acgtcttttt aaatcattat
     2221 atttagaata cttatcttct aataataata attcttttaa aacatccaaa gtatatgtaa
     2281 tcttattttt tgtccttgtc cataacctta ataaatcata aaatctttgc gcatatgaat
     2341 ttcgtaaact aaaaaatatt tgtaaattta ttggagtata tccaacttga atataatttt
     2401 ctattaagtc aagtaactca gcagaagttt taacagtata tgttttatct ttttcattat
     2461 aaatccagtg atatataaaa ctacattctc cccaactatc atcatcttta atatatattg
     2521 ttcctgttct taacttagta aatatcttgc ttattgcttg cggggttgta taattgggat
     2581 ttttcataag ttctctaact tcttcaatag ttattgttat ctctaaattt ttatttttct
     2641 gaaactcata taacaacctt acaaaaagtc tattctctat tgtttgcaaa tcataccttg
     2701 ccataataaa cttattagat ttcatcaaga tattgtcttt ctggtctttt acttgattca
     2761 ttatttttct cctaaatagt atttacaaac taatcaaaag tttagtttat ttattaaata
     2821 atataatatc aaaaccactt gcaaacgtca attttatttt ttgtcgcatt taattatcaa
     2881 agtgtcgtac tttaattatc aaagtgtcgc atttaattat caaagtgtcg tactttaatt
     2941 atcaaagtgt cgcatttaat tatcaaagtg tcgcatttaa ttatcaaagt gtcgcattta
     3001 aaaccttaca taatagtatt ttcaatgtat tcactatcgc taaatactat taaatactat
     3061 taaagactat ttattaaata ctattaaaga ctagtaaaga tacactaatc tattcttatc
     3121 aaagtgtcgc atttaaaaaa taaaagttca tataaataaa tttaaaaata ctgaatcatt
     3181 ttaattgttc ggaatatata cgaattattt gatacttgca tattaaaata ggttttaaaa
     3241 aagatataag tcatctgaaa cgattttaag atgatttctt aataatttat acagaaataa
     3301 atactaaact ccttaaaatg atgacctttc gtctctgcaa cttagaaata tcaatcatta
     3361 cagaaaccaa ttttaaacct gcaattaatg gttttataaa aaacatagag ttgagtttca
     3421 aaaataaaaa aggtttatcg aatttgtaat tttgatgatc ggaaaaaaga aattgataaa
     3481 aataaagttg attaagtata gttttaaggc atttaaaggc ttgtctgaca cgttttaatt
     3541 gcaaacttga tattatttac ctaaaaagat acaaatttgc ttaaaaatgg aaatagaagg
     3601 tcaaatataa aaatagagaa ccaaataaaa ttatattggc tctctattta tcttcttccc
     3661 aatcctcaat aaacttttct aaacttttta atttatttgc agatatttct tcatcatcat
     3721 gtaatgcact tataaaactt tcaaaggaat ttctgtgaaa gaaactaaag aaacttttag
     3781 tttcaacttt taaatattct tctttttcta ttaatgcttt ataatatgta aatctatctt
     3841 tcttaacaac ttctaaaaat cctctaccaa ctaaccttct taaaaaagtt aacatagttg
     3901 attttttcca ttcatatttt tcacctaatg tttttaaaat ttcagtagat gcaacttctt
     3961 catcttcact atcccaaatg tacatcatta ccaaaagttc tgattctgga atctttttta
     4021 tcttcataaa gcacaccacc ttttcaaaag tataactaaa atactataac aatagagtta
     4081 actatacaat actaaatgtc taaaaatgtt attccaattt ttaccaatta atttttcttt
     4141 aaaaaaaatt taagcaggag taaaaaaaat aaaaaattat caaaatttat caatataaaa
     4201 agaaatataa gtataaattt ttctctttgt atattgattg tatacaattc tatttttgta
     4261 aaattaaatt ttacaaaata atcaaaaatg gtaaattttg acatttgcct aatgtcaaat
     4321 taggtgttac aattgtaaca taaagaagat atatatcgaa ataatacata taaaatacat
     4381 ataaaactac gtacgtaaaa tatttttaaa aataatcaaa aatcttaatt ttcaatattg
     4441 aatacaatta aattttcaaa ctcgtctagg gcatgacata caaaagcaaa aaacattaat
     4501 ttagtatctg ataaaaattt aaaaattata aaagggggaa gtctgttatt tttcagaagc
     4561 aatgtaactg taaaaatata aatcagaata ttttgaaaat atgggggatt ctatgggaga
     4621 attaaaactt gaatcaaaaa tgaattgcac taaaaataag aaaaaagttg ctatatctat
     4681 acatcaaatg aaaaaaattt ctaaaaaaga ttatgttttt ttaacagatt gcatagaaac
     4741 attttatatg aaaataaaag aggtttgata aattttcaaa cctcttttta ttgctatttg
     4801 tttttctttt gcaataattc tatcattttc aagatagcat ctctatcttc ttgttctaat
     4861 tcataaagtt tgttagttat ttcttttaca tcatcatcaa tttctaagtc agctaaacaa
     4921 tctttataaa tttcaccttt tccacttctt agccattcct catttacttc aaattctctg
     4981 catatgtcgt taatacttct ttctgtcaaa actctttttc ctttttccaa taaagaaatt
     5041 tgatcttgtg ataagaaaat tctttcccca aacgctctct gcgaaagtct ttcaagagtt
     5101 cttaactcat ttaatctttt tccaatttct ttagctttta atttttttga aacttctgcc
     5161 atttttacaa tcctcctctg tcattactat actataaaaa cacgactttg tcattaaaaa
     5221 aataattata acgacaaaat catgataaaa ataaaaaaga tattgatttt taacgacaaa
     5281 gtagttatac ttaaaataag ttgaacgaca aagtaagtgt aggagggtga aaaatgattt
     5341 gttcaaacaa agaaaaagta gaaacagctt tgatgataga ttatttaaaa aaaaacgaac
     5401 caaatgaatg gagaactata gaaaccataa tctccagcac atacgcatta agtaatttca
     5461 aaaaagaaaa taaagacatt atgaatcaga aataacaaca gtaaatactt aaagggagtg
     5521 gtaagcttga aaatttcaga ttttaataaa aatcaaatag gaacagcagt tcaattacaa
     5581 gatataaggt ttggtacaaa agttgattgc atcattacga aagtagaaaa gaatgaaata
     5641 ctagtcatgt attatgaaaa agagacagag gaaatagcat ataaaacttt gactaaagaa
     5701 gatctgatac ttgatgatta taaacttaag ttattatctt aaaaaataat ttcggagggg
     5761 aggtgatgag atggatctct tgaaggatgc aagaaaaaaa gagtggtttt ggctagagaa
     5821 tgatctagta gatagagaag atttaggaat ctatgaaaaa atgatttata tagttcttgc
     5881 aagatattct gataatgaaa gttgttgctt tccaagctat aaaactatcg ctttgaaatg
     5941 cggatgtagt aaaaggcaag ccaaaagcgt agttaaaatt ttagaaaata aagggttaat
     6001 taaaaaagaa aatagaataa aaagtaactc aaatgaaaaa gaaagcaaca tatattttgt
     6061 tttaacagct aaattaggtg gtgaatatga tgcacaacag gtagtgaata tgatgcacaa
     6121 ccctagtgca cctcatgcac aacaggtagt gcatgaggtg cacagtaaaa agacttataa
     6181 taaaaagact tatataaaaa gtaataccac tccacaaaat gaacctaaaa ctgattattt
     6241 agatctatca ttcttggact tagatataga aaaagtaaaa ctaactaaag atgaatatga
     6301 caaacttata agcaagtttg ggaagaaata catgcatgat aaaattgtta gtttagaaaa
     6361 ctatatcgta aatggtaaag ggagtagata taagagtcat tacagagctt tgttgacttg
     6421 ggggaatgct gatactagca aaggtatatt gcaaccagtt acaaaggcta agaatccact
     6481 cagtggcttt aaagaacttt aaaaactaaa tagtatagga ggtagatagg atgaatgaaa
     6541 aagaagaggc tgtttatttg aatagttcta atgtgaagtt attcttaggt gttagggata
     6601 ttaaggaatt tgaggaattg atagaaaatg tcaatgagcg aatttgtcaa ctccaagaat
     6661 cgattaataa acttgcccaa tttaatattg agttcgaaat taagtcagat atttagttaa
     6721 gtataaatta ggaggttgcc tatgaaagat gaaaagttag aacttactga aaaagattta
     6781 cactgtattg caagacattt acaaaatgaa gttatggaga tggtgtttag agggaatata
     6841 gaagccccta catcatgcga agtttgcgat tacttgcaag aatgcaagga ctatttcacc
     6901 catatagaca cttttataaa attaagtgaa atgacaggtg tagatatttt tactaaataa
     6961 atttagtaaa ttaaaatttt agtattgtaa ttgacgtgct tttttaaaaa gaggacagtt
     7021 gctattagca gtcattattt gaattttgca aatgtaattt ttattacatt cgcattctaa
     7081 aagttttgat ccagtgaatc caggaaaagt tttatattca ccagaaaatt caactttatc
     7141 gtcaataaga gagcattttt ctcttaaggt tttaattttg cacatattca tcaccaccct
     7201 ataattaatt tgaacatact gtcaatatgc tccgattaat tataacatgc aaattatcat
     7261 gatgtagaat taaagcatag gtcaattaaa tttagttata aaatcaatga aacagataaa
     7321 taattaaatg ttaaataaat tagggggttg ttcatgaaag aggaaaagtt agaacttact
     7381 gaaaaagaat tgcattgtat tgcaagacat ttgcaaaatg aagttatgga gatggtgttt
     7441 agagggaata tagaagcccc cacatcatgt gaagtttgcg attacctgca agaatgtgag
     7501 ggagatttta cctgtatgta tcattctttt actaaattaa gcaaaataac aggtgtaaaa
     7561 gtgactgcat ttaagtattg ttttccagat gaaaatccca aagctactat ctaagaagtt
     7621 tttttagaat aggacattct tcattagaag aagagtcgca aatatgactt ccatcacatc
     7681 tagttgacat taacttagat ccaaaagtac ctggaggagc tgaatattcg caagtaagag
     7741 aaatggtttc gtttattaca ggacattctt tgcttataac tcgatattgg ctcatactct
     7801 caccaccttt ctaattaatt taaacatatt ggaagtatgt ttaaattaat tatagcatgt
     7861 aaattatcat ggtgtagaat agtttcaaat caaaagaagc aactaaatag ttaaagacag
     7921 gaggtatgct aagtggaaag aaaaaaacat gaatttacag atcaagatat acaaagactt
     7981 attgaaggat taagaactga tccaaaaata agagaaatga tatttaaaag taaaggttgt
     8041 gaaactagaa caagtacaag aagtaaatgc gaacaatttg ttgaagaaaa acttgcttat
     8101 agaagttatg gatttgaaac tccaaaatac tatgcaaaac ttattgccga cataattgat
     8161 attttagttt acaaaaatgt tccaagagat cgtatttgtg gggtattgta tgacgtcgaa
     8221 agggtgattc ctttagttgt gacattttaa taataagctg ggggtgatta aatgaattac
     8281 tctttagaag cagagcaaaa cattctagga agttttctta taaatgactc tataggttac
     8341 aaaataaggg aattaaaaga aaatgatttc tactttgagt cacataaaat tattttatct
     8401 tgcatgaaga aaataattga ctcaaataaa ccactagatc ttttgctttt aaaaaatgaa
     8461 ttagaaaaaa tagatagatt agtagatatt ggaggcgtta gttacattac cagtttaaca
     8521 actatagtta taactacttc aaacatagat cactatataa aaatcataaa agagaagtta
     8581 cttaaaagac aaatttcaga gttagcaaat gaattaatta ataattcaaa ttcagataca
     8641 agtatagatg aattgattat agatataaac gatttaaaag cacttgtgac gacgagcagt
     8701 agtgtaaatg ataattacat tgacgcatca aaaataaaac gtgaaaaagg cgtacacaag
     8761 tccatagaca cgggttttaa taaactaaac aatatgctag atggatttag atatggaact
     8821 cttacaattt taactggtaa acctgcatct ggtaaatcaa ctatagttaa ccagtttata
     8881 gcacaagcaa ttacgaatgg agagaaagca tttttgtatt ctggagaatt accctctttc
     8941 atgtctatgg attggtttag aaaaactgta gctaatgatt atcacataaa agaatataaa
     9001 agtgtctatg gaggaacata cacagatatt ccagattacg ccgttgagct tatatcagac
     9061 tggatagaag ataaattttt tctctatgat gaagatgcaa taagtgatga agtaaatcta
     9121 ctaaatacca tagagcattt atatttaaaa aaaggggtta ggttctttgt tcttgacaat
     9181 ctaatgacaa taaaaactgg caataaggca gataaatatg agagacaaga gcagatagta
     9241 agcaacttaa aaaatctcgc taaaaaatat aacttagtaa tagttttagt tgcacatcct
     9301 cgtaaaaata tgggtgacat gaagccaact atgtatgatg tgtctggagc aagtgagatt
     9361 gttaattatg cagattatat tttatcaact tacagagttg ttgatgaaga agaagagaca
     9421 gacgacactt atttattaat tttaaaaaat agaataacag gaaaacagaa tataagtttt
     9481 aaaatgaatt tttcagaaag aagaaaaaga ctttacacga ctagtgagga gttaaacaga
     9541 gattacaaat atgatgtaaa taaacaatat gttcaagtag aaattccaga agatgtattt
     9601 taaaaaaagt ataaaaaata ggaaatttta ttaagtaatg attttataag aaaaaccaat
     9661 attgaataat ttaaaaattc aatattgaaa aaaatcattt ttcaatattg aaaaacggat
     9721 tttgaaaaag tattgaattt caatactaaa aaaagtttga aaatatagca tttacaaggt
     9781 ttatataaat taatttcctg acaatgtaca cacaggttta gatatgctag caaaaaaact
     9841 taaaagtagt gtaaggataa aaaggctata taaatttata aaaagattta aaagtgttat
     9901 taaattagag ctaaagttta ttaattaatt tcctcgtaga aaaatattat tttaaataaa
     9961 taaaggagtg attttacatg atagcattag aaaaatttgc aggtggagtt ctaaaagaaa
    10021 agtttaatac agagttacaa aaagttttag ataacattgc agatccaaat acagatttta
    10081 aaaaaacaag aaaaattagt ttagagatag tgtttaaagc aaatgaagat agagatcttg
    10141 ctgaagtaga tataaaatcc aaagcaacta tagtagaagc aaaggctaca acaactaaag
    10201 ttctgatagg aaaagattta gagacaggaa gagttgaagc gtctgaattt aaaaatcaag
    10261 tagcagggca gttggtcatg gatattccag atagtgaaga aactaatgaa acagaagaaa
    10321 atagtgctgg tgtaattgat ttcagaaatg caagttcaaa gtagtttaag aaagtaagta
    10381 aaatataaat ttaaataaaa aaggagaata aaaaaaatga taagaaatgc aatggaatat
    10441 ttagttaagt taggaaaaag agaaacagta tgtgtagatg ggtttaagta cacaacagat
    10501 gcattagaaa taataaaaga accaaaagct aatgaattag aaataactac attgagtggt
    10561 ttagtagatt atataaaaag tggcatagat cataaggcag atgagttgtt attaatacaa
    10621 gttctaactc caagaaaagt gcttttaaaa tctcctttga gaaaaaatag agatagagag
    10681 acatatattg aatgtgttgc actactacca gagaaaagat ttgacagacc tttagactta
    10741 gatagtttta acataatgct acaatcagca tttataaaga ataatgatag agatacactt
    10801 ttaaaaattg caggaaatat ccaagaatct acagtaaaaa atgtgggaga tgatggagtt
    10861 tctcagtctg taacaataaa aacaggcgta gctagtgtat cagaagcaat agtcccaaac
    10921 agagtaaagt taattcctta tcgcactttc caagagatag agcaaccaga aagtgagttt
    10981 attttcagaa tgagttctgg accagcagca ggattatatg aagcagatgg tggagcatgg
    11041 aaaaatcaag caatgttaaa cattaaagcg tacttgcaag aagaattaaa agattttaaa
    11101 aatgtaaata taattgctta attgaaaatc taagagggct tacaataagt cctcttagta
    11161 aaaaaggagg ggattagatg aaaatgattt ctcatatgaa cattttagaa agagcagaat
    11221 ttacagataa aatagctaat gcaatcatgt ctgcaaataa agaaattgaa agaggctgtg
    11281 cctttaatga agcagttgag attgtaaaac aaatggaata tagagaaaat aaatacaatg
    11341 aaggagatat tgcaatatga ataatgtagt tttagttgga agattagcaa gagatccaga
    11401 attaagatat ataccagagt atggtactcc agttgctact tttgcattgg cagttgatag
    11461 gggttataca aaaaaggatg gaactagaga agtcgatttt ataccaattg aagttatggg
    11521 aggatccgca gaattttgtg ctaattatct cgcaaaagga agaatggttt caattcaagg
    11581 ccaaataagg atagaaaaat atgaaaaaga tggagagaaa aaaactttca caaaagtaag
    11641 aacaaaaatt gttaatgcgc ttgatcgcaa gccaaaggat gaagaaaaag aaattagttt
    11701 tcaagtttta gatgatgaag atattccatt ttaaattgga tggtgattat gtgagaagtt
    11761 tagaggagag aaataaatta gttgaagaaa atataaattt tgtatggttt attataagaa
    11821 gatatttcaa agtatttatt tcaaaatatc catatcttca agatgataca ttttcagaag
    11881 gatgtattgg attatgtaaa gcagcagatt attttgatag taaaaaaggt attgaattta
    11941 ccacttttgc agctgtgtgt ataaaaggtc aaatatttaa atttataagt aggtatgtta
    12001 aaaaacatta cagagcagat atagatagtt ttgaaaaatg catctataaa aatactcatg
    12061 gtgaagaaat aagaatagaa gatagattgg ctggtaatga aaatgtagat atagaaaatg
    12121 ttcatgttat tagatcttgt ataaaaagaa gtaaaataga agatatacag aaaataacat
    12181 acttaaggga aaaaggatac actcaaaaag aaataggcaa aatacttagg actaaccaaa
    12241 tgacaatatg caggaagctt ggaaaattaa aatctgaaat taatatacta ggaaaataat
    12301 ataataattt aagttcattg gaggcaaaat gaagactaaa ataattggtg gtagggaaaa
    12361 tcagtgccca ctttgtaatg gtagtgtatt taagattgaa acatcaattg gaatagtatg
    12421 gcaatgtaag gattgtggat gtatgtatca agatatgaat tgtaaagaaa acaaaaggag
    12481 gcattaagta tggcttgtgt tataaagtgt gatttttgtg gtgaaacaat agcagaaatt
    12541 ctaaacataa aggtattcag aattgatgat gaaggtaata taatgagtat cggaaaagat
    12601 gtatgtgaca aatgttatga cagattattt agaagtaact tgaataagaa gatgactaat
    12661 tatgaaaaga taaaaaatat gagcaaaata gaaatggctg aatttcttgc aaatggcgga
    12721 agtggatgta ctagttgtgc ttatgatttt caagaatgct taggcggatg tttagaaggt
    12781 agaaaaaaat ggcttgaaag tgaggcgatg aaataatgaa aaagacgtta ataatcgtag
    12841 gagtagttat ttcaatatta attatgtttg cttgcttgtt cttagtaact aaaaataaag
    12901 caattgattt agaagagcag gttcaatcag caactgcatc tgtagaggtg caagaaaaaa
    12961 ggagaattga tttaattaag aacatagtag actgtgtaaa agactatgct aaatatgaaa
    13021 atagaacatt aaaagaagta actgacatga gagtaagtgc aaatgatgaa tctatcaagg
    13081 aagcaaaatt aaatatacaa gcagtagcag agaagtaccc agatttaaaa gctaatgaaa
    13141 attataagca actaatgagt gagttgagta taacagaaaa tatgattgca caacatagag
    13201 aaaattataa tatgcaagtt cgcaattaca ataaatatat aagaaaattt ccagcaaatt
    13261 taattctaaa tagtatggga tatgaaaaaa tagaatctaa atatactgaa tataaagcaa
    13321 aagaggatgc acctcaaaat ttatttgatt aagaggtgca catatgaaat ttaaaacttt
    13381 tgaaataact aagagagaat ttttagtgag cataataatt gcttgcatga tgattgtgct
    13441 tggatttttc attgatgata aagttcaatc aaaattttta gaagataatg aaagatatta
    13501 caaagcaact aaaataaata atgacaaaga tctctttgat tatgctatgg aaacaagtgc
    13561 gggagattct ttgataagtg gaagatttaa aagtgtagat tctgtatcta tagaagaatt
    13621 aaaaggggac tattggttta tagaaagaac ggaagaaaaa tacactatgc atactagagt
    13681 ggttaccact aaagacagta aaggccattc acataaatat actgaaacat attggacttg
    13741 ggattaccat gattcaatag agaaaaaatg taataaattt aatttcatgg gtaaagagtt
    13801 tgatgcgaaa gtaattacta atttatctaa agagagattg gcattaaaga aagatatatt
    13861 taaaaataat aatcttaaaa taaaaggtaa ttatgcttac attaataata agagaaggta
    13921 ctattatgaa gttataccga aagaatttaa tggtactatg tatgctaatt taaaaaataa
    13981 tactatagcg aatccagctg ataaaaacat aaaagttttt aagggtgagt ctatacagaa
    14041 tgttttagaa aataataatt ttaatacagg gttttttaaa gtagtgtttt ggatcatctg
    14101 gatattaata actacaatca ttatatatgt attttattat ttaaaaaatg attggcttga
    14161 agataaataa agatgtaaga ggagaagatc aggtatgaaa tgtaaggtgt accatgctat
    14221 taaaaatata aaagatgcaa atgagttaat tgatagattt gaaacggaaa atatattctt
    14281 tgaattaaat gacatagata atgaaaatgt cttaaatgga gaaatgaatt taactgatat
    14341 agaagatatt gcatcattgc aatttatatt agatggagat ttatttatat ttagtagttg
    14401 tgatgaaatg atatttatga ataaatacac tcaaaaagag ttgggacttc aataaatgaa
    14461 tgaggggaga tactaaagat ggatttaaag ttaattagca gtatacctaa ttggaaggta
    14521 gaagatcagt tggcaaaaat tgatgaagag gtaactgagt ttaaaaatgc tatagataca
    14581 ggaataacta aagatataat tgcagaagga ttagatgttt gtcaaactat tttaacaatg
    14641 tttcaagttt tagaaataga aaactatatt actgaaggag tagagacaca taataagaaa
    14701 ctaaatggaa gaggatggga tctcaaagaa ataggagacg atatacttaa tttaattaaa
    14761 aattcaatga ttaatgaatt gattaatatg aacaagaaat taaaatgcct tgatgaaaaa
    14821 gaatttaatg caaaaataca atttttagat gtagataaac tatggaattt gtgcatgcct
    14881 ttagttaact taagtactat aaaaggagtt aatgaaagca ttgatgaaat gaaaatggga
    14941 gagtttctaa aaaatattaa ttaaagtaag gagataatat aatatgttga tagaagtaac
    15001 tgaaaataat agagaattaa aagtaggaga tattgttgaa attaatggtg gtaataagtt
    15061 atatatgatt ctttctttaa aggaaacaat aggtgggtat gttattataa atatgcagaa
    15121 tggatatggt tcatttggtg cttataaaag tttaagtcaa ctagaattag acttgaaggt
    15181 aagaggttat aaattatata gttctgatga ttacaagttg caattagtgc ctagagaata
    15241 agatatattt caaaaaagaa aaaaaggagc attacttcac gctcctgctt gtcaaaattc
    15301 taaaaccgtc atcacaacat cattataaca taattaggag tgtgaggtaa tgcaatctag
    15361 taaaaaagac aatttgttta gtagtgcaga aggtaagtta tatgattata aaaaaataaa
    15421 agcggaaata gaaaaaatag acattgatat gcagatgata aaaaacgact acagaggatg
    15481 tagtggagtt gaaattaaag aaaagacagg caagacatat gatattaaaa gtgtaattga
    15541 aatagagact gaagaaaaag aaaagaaaat tgccttaaaa gaaaaagaga agagacataa
    15601 agagttgata atagaaaaaa tagataatgc aatgaaaata ttaagtgaag aagaaaaaaa
    15661 gatagtacag tataaatact tttcaagtag caggacatct tgggaatatg ttggcagaat
    15721 gattggtttt tcagcgagta agtgtaagca aatgaggttt gatattatag ataagataaa
    15781 aggattgcta taaaaatgtc cgatttgtga cgattttaaa ccgatttatg tctgttttgt
    15841 gtctaaaaaa tgtccggttt gtgaccaatt tatgtctgtt ttgtgtcttt taatcatgat
    15901 aagattgtat tgtgagaaaa atatatttaa taaaaatggc ttggaattta cgttctaagt
    15961 cttttttatt gtctagagag gtgttgaaga ttaaacgtga tataacagaa gaaatttcaa
    16021 gtgctgcata tgtaccagac aatctaaagt attacgacaa tgtaatgaaa gaaactcctg
    16081 gtatgtatgg aagagaatgt tctttagatt ttataaagaa aaagcaagaa aaactcttga
    16141 aactaaaaaa gaaggttaag aaaaattatg attctaatat taataagata gactcatatc
    16201 taaaaatttt agagaaaagt ataattgatg agtctgatca cgttgagttg gttgtattta
    16261 aattatattt gcaattagaa aatgttgtaa aagtaactga aactgtaaac gacttaggat
    16321 ttagaataaa gactaacact tatgcaagag aaagaaaata tagtacaaat gatataacct
    16381 ctataatcac agatcctttt actgatgtag ttgaggactt aaaaatatta gttcaagaga
    16441 ggcaaagaaa aaattattat ggaaataagg agtgttttta atgaatggaa gcacatagtg
    16501 gacaaaaggt taatcccata aaagatatag aggatgtatt tagactttta aattttctag
    16561 aagaatggaa tgaaagaaac tatttattag ccctctttgg aatgtgtact ggacttaggg
    16621 taggggatat attagcactt aaagttgcag atgtaacaga cttaaaactc gataaaaaag
    16681 gaaataaaat aagagtatct aaggattgga ttagagttat cgaagaaaaa agagactata
    16741 atagagaggt ttttttatct gatgtaataa aaagtgctat agaaaattat actcaagata
    16801 aaccaggaga agaattttta tttaaaagtg gcaagaggaa gaaatttaat agacctatcc
    16861 aaacaagaca agcaggaaga ataataaaag gagcagcaaa aaaggtagga ataaaagaaa
    16921 atgttgcaac acactcttta agaaaaacat ttgcaaggca tatttatgat gaagaagaga
    16981 ataagactta tgcattagaa cttataagga agattctagg acataagaca atagaaatga
    17041 ctagaagata tataggaata gataaggatg aagaagtaaa agcaataact aaatttacta
    17101 ataagttaaa gaaaagaaaa agaaattaaa atatcttgta ttttatctaa aatttaatat
    17161 gttacgtttt tcaacaacag gacattatta gaatgcaaag agccttaaca atagagttta
    17221 caaggaatag aatagattta ttaaaatgtc tgaaaatata tattaaaggt catttattca
    17281 agggatttat agggatgtag atatttaaat gtttatttag aagctgagta aagaggtgat
    17341 taattggatg tgaatgaagt tgaaataatc gatagtagct atgaaaaagg taaagagttg
    17401 acagaagatc agtgcaatat ggtaactcta ttgattacag gtgctacagt aacagaaact
    17461 gccagaatta ttggagtaaa tagaaaaaca atctataatt ggatgaataa agagcatgtt
    17521 aggaaagaaa tggacagacg gaaacaggag ctaacaaacc aaggaaattt aatgatatta
    17581 aaggatttag atagttatat caacaatatc aaggaattag ctagtgacaa gagcgataag
    17641 agggttatgt tggcagccaa tcagtatctt ataaatcgta tatatggaac tcctacaagt
    17701 acagttatac aagcagagga taatagtgtt ggcatgggta tggatgcaac agagatagaa
    17761 gcagctatag ctaagataag aataagaaca agcaagaagt aattagatat gaaataaatc
    17821 ctgcaagaat atgttttaat tatgtttgaa atcaattaaa gcaaaacata tattatatat
    17881 atatttactt taataattaa atcatatatt gtatttaact ttagttatag ctaatttaat
    17941 aaaatttagt gagacttaat aattaaagtt tcatacagtg aagtgatgtg gattattata
    18001 ggatatgtga acctattgga aatgctaggt tctttttaac tatgccgaaa atacatattt
    18061 aaggaatagt tgaaagataa ttaataattc taatcaaaaa aatatttctt gcaagaaaat
    18121 aattcaaaat actttaatat atttattttt tttattaata gggggtgtcc ttctattttg
    18181 aaaaaattat tttttatgga aaatgagttc tagaaatttt ctagcaaaat ttaaaaacca
    18241 gagatgtatt tcaaagaggg tgattttatt agagaaaaat ttacattgtt tattttatta
    18301 attgcattac tcactgagcc taaaaaaata gaagaagtaa gaggaagtaa atacgagtgg
    18361 ataatctaga aagaaatata caattaatat ttagttatct agcaaagact tttataagaa
    18421 atggagcatc tataaaagat gctgaaaaag aagcaaatga gattataaaa gccaattcaa
    18481 acaatctttg gggagttaat ggtctcgctt atcaacttgg aaaaataaac ctagaatttt
    18541 tctgtatgta ttttatgcaa gacacatatt taccaaaaga agataatgct gctgctccta
    18601 tagctaaagt tcatcatgag ctatgggaag atatacaaga atctataatt ggagatggtt
    18661 cacagcaatt aggaagaatt tatccaagag ggacaggtaa aagtgcattt ggagatttag
    18721 cgacaactgt atggtcgcat tgctataaac ataagacata tactttgatt tgctctgaca
    18781 taggatctac agcagagaaa tttgtaaaag atataaaaaa tgcattactt gaaaatgagt
    18841 atattaaaaa agcatttggt gtccttttaa atgataatga tagaaaatac atttgtaata
    18901 gtactcaact agagctaaca aataaaactt ttattgaagc tatatcatct tcatcaccca
    18961 tgagaggaag aaaatacaat aataaccgtc cagatcttat catacttgat gattatcagt
    19021 cagaagaaaa tgttagaaca gaagatgcta gagagaaaaa atttaaaaga ttttctgatg
    19081 atgtaaaata tgctgctcaa aaaccagtca taagaaatgg taaaactata aaaagaggaa
    19141 caacttttat agcattagga accttacaac acaaagaatg tttttatagt agactaaaaa
    19201 atttaccaac ttggaagttt aaatgcgaaa aaggagtttt agttgataat gtagatgaat
    19261 tatttaattc tggtttatgg cttaagttca aagagatctt atttgatttt aaaaatacaa
    19321 atcatcttga agatgctaaa gaattttact gggaaaatga aaaagaaatg aaattcccta
    19381 tattatggcc aagtttttgg gattgtttag atatggctct aagttattat gaaaatccaa
    19441 catcttttaa gcaggaagtg caaaatgatg tcaattctat aggagaaaaa tggtttaaaa
    19501 ctgttagaac agaaactaga gaagaaattg aatcgcatac ttttaaaaag acaatgctgt
    19561 tatgtgatcc agcatctgcg ggtgggtcga aacatgacta tagcgctttt cttgttggaa
    19621 gtgaatcaga aaatggttta ctatatggaa gattggcaga actagctaaa ataaatgcta
    19681 gaactgattt tgataaatat atagaccata tgatttattt attaaaagtg tattcagata
    19741 taacacatat ttatatagaa aaaaatacat ttaatggcgc agatgccaac caattagagt
    19801 ttaaaataaa aaatgataat gttcttagtt atagagatat agaaatcatt aatgagcaac
    19861 aaaagaaaaa taaagatgat aaaatctcta ctttaatacc tgttttaagc aaaggccaaa
    19921 tgatttttgc agaagaagat aaggaattta tacagcaaat acttgatttt acaggacaaa
    19981 aatactctct acatgatgat gctccggata tatcatcgga atttattaat cgaattaaca
    20041 acataaaagt ttctgagaca gtaaatatct ttgatagaaa attattaggt ttttagttgg
    20101 gaggtaattt attggctaat agatcaaagt ttgaagaagt tcaaaaaact ttaactaaaa
    20161 aaggaaagaa gttcaatgtt ggaataggaa aagatgaaaa aggatatttt gcatatactc
    20221 atagagcaag gagtaaaagt tatatttcaa agcaagatat tccaataaaa gttttaaaat
    20281 ttatagagtc tacaggatag gatgtgttgt taatggaatt agatttagac ttaataaaag
    20341 acatatatga cgattggaac tctaataaat ccgaatatga tactatgtat aagtattata
    20401 aaggtgaaac ggatgctatt agcaactata aaatggttac caagagatct aataataaga
    20461 taaacactaa tttcttaaaa aagtttatta atgaagaagt cgcttattct cttgcaaata
    20521 agattaccta tacaagtaga tctggtgatg agaagattat aaatgattta gaatactata
    20581 cttgccattg gagtaaaaaa catgactcag atttattgag gtatgcacta ttatttgggc
    20641 tttgctatga actttactat gtaaaagatg atgaaataca agtaaagata atcaagccaa
    20701 cagatggctg tcattatgaa aatgaaaatg gagaaatagt atgctttatt agagagttta
    20761 ccaaaggttt cgaagatgac acgtatgtag atgtatatga caaagaatat atatatcatt
    20821 ttgattcaaa tttcaaagaa gttgaaagtc ctacagtaaa caatatattt gatggaaatg
    20881 ttccaatatc aatttgcaaa agaagtgaag aactaggaaa aaatacaata tttaatgata
    20941 taaaagggtt acaagatgct tatgaaacta atttaagtga tataagtaat gaaattagtg
    21001 attttagaaa tgcttattta accttttctg gatgtaatat taaagaagat gatttgccaa
    21061 ggatgaaaga acttggaata cttcaagtta atggagatgg aaaaatagag tggcttatta
    21121 aagatatgaa tgatacattt gttcaaaata cactatcttc aataaaagaa aatatgtatg
    21181 aaattacatc tcatataaat cacaatgaaa aaatgcaaag taatacatca agtctagcag
    21241 ttatagcaag attaatcagt acagaatgga tttgtagcca aaataatgat agtattgcag
    21301 atactttatt taataggtac aaattattgt gcatttggct taataaaaaa tatggttttg
    21361 actatgatta taaagatatt aaggctaaat ttacccctaa gataccacgt gatgatttag
    21421 ttgtagcaaa tatattaagc caacttggag ataaattatc tactgaaact ggcttatctc
    21481 aactaagttt cattgataat cctcatgctg aaatagaaaa agctaaaaaa gaacaggaaa
    21541 tagtttcaga gggagagatt ttattagatg aatcaaaaga ttataactaa attgactgaa
    21601 gatatatata aatctgctga aaataggaca aaaccatttt ataagtatca aagagaaaat
    21661 agggataaac ttttattaga aatagcaaag atattgctaa cacacgatat tattaatgaa
    21721 tacttaaata ttaataataa agataagaaa aatctaaggt caaaacttaa taaaattata
    21781 aatgagtatt ctaatagtta tgatgaagaa gtcaaggata tagaaactat tttatctgat
    21841 tcatctaaga ataaatattc aaatttgata attttattag gagtaatact gaagataaaa
    21901 gatgttagta atataaacaa taaagttata aataagatag tgaataataa ggttgatgga
    21961 aaacattgga gcgatagact ctggaagaat aaaaaaggta tagaagagtc cttaaaaaaa
    22021 gaaataaaga gttttctaaa tggaaaaact tctgtaaata agatagaaaa agcaataaga
    22081 gataaatatt caacaggtgc aggacaaacc agcagactaa tagaaaatga agttgccaga
    22141 tgtcaaagtg aagtaaatga gtattttttc aaggttcaag ggattataaa agtaatgtat
    22201 tgtgcagatt tagattctcg cacatgtagt gattgttcta tgcataatgg agtgatattt
    22261 tatgtcaatg aagataggcc ctctttgcca agacactgtc actgtagatg tcaatatata
    22321 cctgtttaac atgtattaat taatttaata ggtgttttta ttatgtctaa aaaatatgag
    22381 gtgaaatatg aaagttgaaa tattaggaac agaatatcta gtgatagaga aacttgaaag
    22441 cgaagatgtg ctattaaagg aaagagctgg atattgtgat cactctgtta aggaaattgt
    22501 tatagagaaa ataaattctg aagaaggttc attgaaagat ttaagtgtat ataaaaatga
    22561 agttgctagg catgaaataa ttcatgcatt tcttagtgaa agtggattga agagttgtag
    22621 ttcttgggct acaaacgaag aaatgattga cttctttgca attcaatttc ctaaaatagt
    22681 aaaggttatg gataaaatag aatgtttgaa ttaaattttc aaaaaggaga actttataca
    22741 tgttaaaaaa agaagtgttg gaattactaa aagaaataga agatgatgca gaaatagatt
    22801 cattgctgca atcaacagat ttattcaaat ctgcatctga taaaaagctt acaatagaag
    22861 agtttagaga attaattaat aatgatgcaa actttaaagc tattatagat aatgaaaaaa
    22921 ataaatatca cagcgaagca cttgaaaact ttaagaaaaa agatatgcag acacttataa
    22981 gtgctgaagt tctaaaaaga actggagcaa atgaaacaga agagcaaaaa gcaataaggg
    23041 aattaagaga aagcctagac aaactaaaaa aagaaaagca gtacgcagaa aaagtttcta
    23101 aatataaaga tattttagtt gaaaagaaaa tacctacaaa cttaattgaa tatctattat
    23161 ctgatgatga tgataaaaca aatgcaaata tagagatatt tgaaaattca atgaagcaat
    23221 atgtgcagtc aaaggtagat gaaagaatta aagatggttc atacgttcca cctggcaaag
    23281 atagtgcagg ttcgctatca gaaattagaa aacaaataaa gcaagggcta aatagtcttt
    23341 agtataaaaa aagaaaggat gattttataa atggctaatg tattacaata tgtaacactt
    23401 tttcaacaag aactagatat gcaggtaaca gttggctcaa ctactggatg gatggagaaa
    23461 aatgctggtc aagttatata taatggagga agagaggtaa aagtaccaaa agtaacaatg
    23521 gatggcttag gtgattatga cagatctaaa ggatatacgc aaggcgcagt tacacttgag
    23581 tacgaaacaa tgaaaatgga gatggataga ggtaggactt ttatgttaga ttcaatggat
    23641 gtaaatgaaa gtaacttcgt tataaatgca tcttctgtaa tgggattatt tcaaaagcaa
    23701 catgttatac cagaaataga tgcatataga tactcaaaga tattttctga aacagctaaa
    23761 gtagatgagc gtgtaaaata ttcatacgaa gctacagtaa ctacaatatt aagtgaattg
    23821 aaaaatcaac ttgcatcaat acaagattta tctggagata tacctcttat tattagtatg
    23881 tctagcataa ttgcaagcat attagaacaa tcatctcaac ttgatgatat tgaatttggc
    23941 gatggagata taaaaagaat tgtaaagtct atagacaatg tacctataat aaaagttcca
    24001 tcaaaaagaa tgaaaacaga atacgtattt agagatggta aaacatctgg acaaacagag
    24061 ggtgggttta ctgaaggagc aaaggcttta gatataaact ggttaataac tccagcatct
    24121 gctccaattg cagtatctaa gactgataag ccaagaatat ttactccaga tcaaaaccaa
    24181 acagcagatg cttatcaaat agactataga aaatatcacg atttgtggat attggatgaa
    24241 tatttaaaac tttgtagagc atctgtgaga caagctaaag ctgcacaaga ataaggagtt
    24301 gttttaaatg tttgcattga taaaaaataa cataattaaa gttgttgaag atgaaaattc
    24361 gagagataaa ttgatttcaa aaggatttaa aatatattca gaagaagtga ctaaagagaa
    24421 tattgagaaa aaagaagaaa aagttaaatc aacctctaat gatttattta atctatctaa
    24481 accagagctt atggaaattg ctaaaggcaa gaatataaag ggatattcaa ctaaaactaa
    24541 agaggaatta attcaactac tagatgagta caatgcttga taatgttaaa ttagttttag
    24601 gaatagaaaa tgatagatat gacaaattaa taatccttta tataaataaa ataagtgaga
    24661 tggttttaga atattgttct aggcaggaac taagtacagc actagaaggg tttgtagaag
    24721 aaaaagtaat atcaatatta tcttcaagat ttgaagaact taagaaaaat agtggacttg
    24781 ataatctaga aaaaagctta aaagctataa ctagaggtga cactaagata gaatataatg
    24841 ctttaacatt acaaactgct gaaaatagca tcagttcatc atctttttta actgatactg
    24901 ataaaaaatt tctaagccca ttttgtattg ttagtatgta ctaaggggtg attaaatgac
    24961 tgaagcagat atattagaaa tgacatacct tgataaaatg actataatta gaaaattaaa
    25021 aggctggaat gaagaaacat gttctaatga ttttagtaat gttgttatag ctgaaaatat
    25081 cccatgtgca atttctagaa aagaagaagc cattgtaaat ggtgatattg gatctatagt
    25141 tgtaaataga aagttatttt gtagaccaga agtagatata aaaataggtg atactataaa
    25201 tacaacctat aatcatggag aagaagctgt atttaaggtt tctaaggcaa atatatatcc
    25261 aagccatatg gaaataccta ttacagaatt ggagaggata taatgggaat agataattta
    25321 ggagtctttg aagcattagc cttagattta ggtctaaatg catcaaactt taatagaaaa
    25381 atcaagaagt ttacaaaaga aactgggcaa gaactagcag aaaatataag agatgaaact
    25441 cctgtaaaaa ctgaaaagtt aaaaaaggga tgggaagaaa agcaaccaga atatgatact
    25501 tatattgtta aaaatgaagt tgattatgct actttagtaa ataatggcca ttcaactaaa
    25561 aatggtggca ttgtccctgg ttcttttatg gttgaaaagg gaattgaaag aacaaaagaa
    25621 aatatgacaa gtgcaaaagc aaatttattt gattggtagg tgattagttg cttacaaaca
    25681 gagacattat aatagcagtt aataaaagaa tcgaaaatga gtttagatat atagatattt
    25741 ttattaatga ggataatata caaggctttg aggaggcttg tttttttgtg caacttttac
    25801 ctgtttctaa taatattgca aataaagttt tagatactaa aaagatattc atagatattg
    25861 aatattacca aaaggctaga aaaaataaat taaatctata cgacatacaa agtagattag
    25921 aaaaaatatt taacagaaac attagagtta aagatagaag tttaatcatt gagaatatat
    25981 ctccatcaat cgcaaaagat cctgtaggat ataaactaac ttttctaatg actgtatctt
    26041 attttgaaga agtctatttt gaaaatgaaa catttgaaat tatggaaagt gtagatttag
    26101 agataggaag tgattaaatg ggtttaccag aaattaatat ttcttttatc caagcaggaa
    26161 aaacttttgt taaacgctct ggtggtattg tagcgcttat tttaaaagat acaaaaaata
    26221 tagggttagt tgagataaaa gaaatagagg atattccaga aaattattct gctgctaatt
    26281 tagattatat aaaaatggct atgaagggaa atgtatatcc tccaaataaa gtattagttt
    26341 atacattaga tacagatgaa tcaatagatg atgcactaga ttttcttgaa acttgtgagt
    26401 ttaattatct ttgtatgcca gatgagacag aacaagattt gcctaaaatc aaaacgtgga
    26461 ttaagaaaat gagagaagat aataaaataa aggttaaagc tattactgca agtaattcag
    26521 cggattatga aggtataata aactttacag caactgatat tgaagttgaa ggtaaaaaat
    26581 acacttcaaa tgaatttctg ccaagaatag caggttttat tgcaggaact ccatcaactc
    26641 aatctgtaac atatgcagaa ataccagaag ttactaatat acctaagcta acaagagctg
    26701 aagcaaatac aaggataaat aatggagagc taattttaat aaaagaaagt ggagctatag
    26761 taatcgctag aggagttaca tcctttacaa caattactga ttcaaaaggt gatatattca
    26821 aaaagattaa gttggttgat acactagatc aaattcataa cgatattaaa aaaataattg
    26881 taaaaaatta tataggaaaa acccaaaaca cttatgataa taagtgttta cttattgttg
    26941 caatacaatt atatcttcaa gaattagaaa aagatggttt aattgatgaa ggttcaactg
    27001 tagaaattaa tttagatgct caaagagcat ggctaaaaaa acaaaatata gatatatcta
    27061 atatggaaga acaacagata aaagaataca atactgacac tcttgtattt ttaaaagcaa
    27121 gaattaaatt aatagatgca atggaagatg tatatttaga catctctatg tagtttggag
    27181 gttaaaattt gaaagctaaa atagatgcaa aaagaataat atctggtact tttggagagt
    27241 gttggctaga taataagcaa cttacatcaa caaaagggat ggaggccaag gttgatatag
    27301 ataaagaaga aataaaatta tgtggtcaaa taatgaccac atataaaatg attggtgcaa
    27361 ctggaaaagg atctataact ctttataaga taagtacctt atttggcgag tatgtcgaaa
    27421 aaatgttaaa tgaaggtaaa gaatttaaag taactataat aagtaaattg caagacccag
    27481 attcatatgg ttctgagaga attgcacttt atggatgtat gtttgacgac ttaactttgg
    27541 ctgattggga agctgcgaaa gttggagaaa tagaagttcc attcacattt gaaggctttg
    27601 aatacttaga taaaataaaa gaaaaatagg aggattttat tatatgaaca cagtagaaaa
    27661 attattaaat atggatgcag gtaaattaaa aatgccatct agcgcatttg aattatattg
    27721 taaaaaacta gatgatactt tagaaataga gtgcaaggct attgatccag aacgttttga
    27781 cgaaataaga atgaactcta tggacgttaa tagtggagat gtagaaaaca ttaacatata
    27841 tgaacttaaa gtaaatacaa tattagaatc atgttctata tttagcaata tggaattagt
    27901 taaaaaattt agtgcaatta cacctaaaga attaattaaa aagctattac ttagtggtga
    27961 aatagataaa ttatacgagg aggttaataa agtaaatgga atagatgcag agggagataa
    28021 aaaaagacaa aaagaaagag ctaaagatat aaaaaactaa ttaaaacgga tggagaagtt
    28081 aacttgatgt acttattatt taagtataaa ggtataatgc catccgtttc ttatcatata
    28141 ctaagaggcg aaaaagaaat tgttagtgtt tttatggatc aagaattaga tgaaagaaaa
    28201 gaagaaataa aatctggtgt tgggaggttg ttttagtata tgagttctaa tacaaaggtc
    28261 ctacaagcaa taataaaaat gagagatgaa gcaagttcaa cattaaaaag agtagcagca
    28321 aatactagag ctctacatga ttcatcttcc caagcatcaa gagcattaaa taatgttcaa
    28381 agtaatttaa aaagtgttgg aactagtgct ttaaaagcag gaggtcttat cactgctgga
    28441 gttggtggtg catttgcagc tgcaactaaa gttggaacag aatttgaaag cgctatgtcg
    28501 caagttaagg ctacatttgg aaattcctta aaaccaggag attttgaagc tctgtctaag
    28561 gctgctagag atgctggaaa agctacaact aaaactgctg ctgattctgc taatgcactt
    28621 cagtatatgg ggcttgctgg atgggatgtt caaaagtcaa tgcatgcctt aatgccagtg
    28681 cttaatttat cagaagcagc ctctatggat cttggtctag catcagattt agtaactgat
    28741 agcatgagtt ccatgtgctt agaagttgat aaggtcagta aaagtggaaa agtactagct
    28801 ggtgcaggtt taagtgagta tttagataaa gttgcaaatg catctactaa atcaaataca
    28861 agtatacaac agttgatgga agcgtttaat gtcgcaggtg gtacatttag caatttaaaa
    28921 gttccacttt cagaagcaac tgctatcatg ggaatacttg caaatagagg agttaagggt
    28981 acagatgcag ggcatgcttt aaactctatc atggtaaatc ttacaacagg tgcaggtcaa
    29041 gcaggtaagg caatgaaaaa gttaggtgta agtgcatttg ataatcaagg taaatttaaa
    29101 ggtatgtcaa atgtgcttaa agaggttgca aataaaacta aaggtatgac tgatgaacag
    29161 aaaaattatt acttatctgc tattggtgga aagactcaat taaaaacgtt acaaaagtta
    29221 cttgatggtg tgggaaatga atatgatagc ttaaagaaaa aaatagataa ttcaaaaggt
    29281 gctttaaaaa caatgtctga tgtaatgaag gataatacta agggtggact tgcaacctta
    29341 aaatcagccc ttgaagaaac tggaattact atttactatc aactaaagcc agcaatagca
    29401 tctgtagttg ataatttaac taaaatgact gtttggttta ataatctaag tgaaccaata
    29461 aaaagagtca taactaatgt aatgctttca gttggagcat ttggagtact tttattaact
    29521 ataggggctg tgtctttaat tgtttcaaaa gctataggta tatttggctt ttttgttcca
    29581 ataatattaa gagcaaaagt tgctttcaga gcttttgtaa ttggtatgaa accattaagt
    29641 gcagtatttt tagctgtaat gggtcctgtg ggatggacta taacagctgt aattgcatta
    29701 tcagtagcat ttaccattgc ttatgctaaa tctgaagtat ttagaaataa ggttaattca
    29761 ttagtaaaat catttacaga atttgcaaag cctatagtaa atttttgtat gcctgcactt
    29821 aaacagctag gagagaaatt taaagagttg ttatcagcat ttggtccact atttgcatct
    29881 attttaaacc taggaagtgt aataatgaag gtactaggac ctgttttaat cggcgtaata
    29941 ggttttcata tagcaaaatt agttttgatt ttatctactc tggcaactgc ttttaaagct
    30001 gtagttgata ctataactaa tgtattaaat ggtctagcat ctattttaaa tggagtattt
    30061 gatttaatta taggtattgt cacaggcgat agtaaaaaga tgatggatgg agttaaacaa
    30121 atatttgatg gtggaactaa aattataaaa tcaatttgga aaggtttcat agattttgta
    30181 actgctccta tacaagctat agctgatata gctgacagct tatttaaaga taaagctaaa
    30241 tggcttacag ataagtggag agatttaaga acattcttag aaaatccaat tgaagcaact
    30301 gccaaaatat ctactaaaac aaatgtaaat atcccaggaa gtaaagcagc agatgcagct
    30361 ggtaaatcta caccaaatac aggtggatta aaacaaacag tagcaaatct aaaaggttca
    30421 tttagtgatt ttaaaacttt tattaagcct atgacagact ctataagtca aggagttgaa
    30481 tctggaaaaa ctaaatttac agaactgcaa aataaaatat taaatgatgt taaacctgca
    30541 ctaaaggtcc tagaggattc tggagtaaat tttaaggata agtttaagga gtcaataact
    30601 tcaataaaag ataaatttaa tgaattaaag aacatattag ctggaaatat taaaacaaat
    30661 atagataatt tagtaaaagc atttgaacct ttaaaacctc atttagataa tttgaaagat
    30721 tgctttgata aggttaaaaa agcgattact gattttttta ctccagccaa acaggttgga
    30781 gatacagtaa acaaagtaag ccaaccaatg gatagtgtta aaaagtctac tgatggaatt
    30841 aaactttctt tccaaagttt aactcaagca cttgctcctg ttaaagcttg gtttcaagga
    30901 tttatgcctt tcctatcaca aataggacaa caacttttac catctatagg aattgcaata
    30961 gctggtgttg ttggcggaat aattttagca tttacatggg ttttaaatac attgaccgca
    31021 ataataacaa cagtatctgg aatcataaat ggaattatgt cttttatatc tggaattatt
    31081 aatgttatta tggggcttat aacaggaaat gctgatcaaa ctatggatgg acttaaacaa
    31141 atatttgata gtggagtagg tgtaataaaa tcaatttgga atggccttgt tgatctagtt
    31201 agtggcgtac tagaacctgt aatagatctt gctgattctc ttttcaaaga taaagttaaa
    31261 ggggtaaaag aagcttggaa taaacttaaa gatttcttta aacatccaat taagggaaca
    31321 atagaaatat ttaaaaaagg tcctagttta caaattggaa aaggtgcaga tgctgctggc
    31381 aaaagtccaa gaaaagcatt tggtataaat agagttccta gaaatgatta tttaataaga
    31441 gcacacgaag gcgagaaact tttaaccaaa caagaagcta atcaatacaa aaggaatagt
    31501 tcaaatggaa gtttatttct tgataaattg gctgatacaa taattgtaag agaagaggct
    31561 gacatagata aaattgtaag aaaaataaac aaaaaaatat ctcttgcaaa aattggaggt
    31621 gttttgtaaa ttatcgtgct ataattgtag tatataattt acagggggtt atgttatgaa
    31681 taaaaaaata ctagtattaa caatatcaat tatactatct ttaagtttag ttggatgcag
    31741 cacaaataat tcaaaaaata gagatagtaa atctaagcaa aacacagagg aagttaaaaa
    31801 tcataaaata ggcgaagttg ttgatttaga tggagcaaaa gtatctgtta aaaatattag
    31861 gaaattagaa acaaatagta atgattatgc tgttgaaatt gaaataacta atacaggtga
    31921 taatgattta caaagtgatt atctagaaaa ctatatatta aaatcaagtg acaatactac
    31981 tggagaagca ataaataacc aagaattaaa tggagagtta atgtttgata caatacgccc
    32041 aggagatact ttgaaaggtg aaatagcatt cgaacttaca aaagatgcaa caccaaaaac
    32101 acttgaaatt gcttatgaaa atgtaggcaa atatacagta tttgatttat agtaattatc
    32161 ttaaaagacg cttatttact taagtgtctt ttattttagg aggagtttaa tggaaatatg
    32221 gttaagacaa gcaaataata catttaggtt tcctgttatt ccaccctctt ttgagataaa
    32281 tggaagtgcg acaataaata catctaatat tttaagtgtt ggagatatcg cagtatttgg
    32341 aggattaggg cttaaaacta tagaactatc ctcttttttc cctaatcaag aatatagttt
    32401 ctgtaattat aatggatttc caaaaccata tgattgtgta aatttaatag aaagttggat
    32461 gaaagaagga tatatattaa gatttatcat tacggaaact aatataaact ttgaatgtat
    32521 aattgcagat tttaattata gagaacaaga ttattcaaga gatatttatt ttaccttaag
    32581 tctaaaagaa tacagaagga tacaaatatc caaagtaaac attaataatg atgaaaaatt
    32641 atcttctgaa aagaatgttc ctctaacaaa aggatttgat actaagcaaa aaacgcataa
    32701 ggttgttgaa ggagatactc tttttaagat agctaaaaaa tattatagca atggagattt
    32761 atgggagaag atttataaag caaatgaaga tgagattaaa gatccatctg taataaaaaa
    32821 tggttgggta ttaataattc cttaaaaatg gtggtgattt ataattaata aaatcaaaat
    32881 acaagttcat ataaaaaacg gaaatatata taatgtaact gatttagtgg ataaatgcac
    32941 ttggtctggt gattatagat caccatcaag aactttggaa ttttctataa ttcaatctgc
    33001 aagtgatatt aattttaggc agatagatat tccagtagca agtacagttt gtttctatgt
    33061 agatgataaa gaacttttta gaggaatgat aatagataga tctaaagact ctagcaataa
    33121 taatatagat tttacagcta aagatatggg ttttttactt ttacaaagcg aagtgtcata
    33181 caattttaaa gataaattag tcgaagatat tgcaaaacag gtttttgtag ataataaatt
    33241 cccactaggt aatttaccta aaactaacgt taaatataca aaaatgttta ttggtgtaac
    33301 tggctacgat actataatga gcgtttatac agaagcaagc aagacaacta aaaaaaagta
    33361 tatgatagag tctaatcttg ataaatttaa tgttattgag aagggtgtag ttactttaaa
    33421 tataacattt gaagaaggct ttaatcttat caatacaaat ttttctgaaa gcattgagaa
    33481 tgtaaaaaat aaagtactag ttgttgatca gtatggtaat aaaattagtg agaaaataaa
    33541 tgactctata tttaaagacg ttggggtaat tatgcaaaag gtaatacaac aacaagaaaa
    33601 cagtactata gacatagatg gagagtttaa aggaatcgaa aaaacttgct ctttaaaagg
    33661 atatggtgat ataacttgtg taactggtag aggtgtaaaa gtaaaagact catatacaaa
    33721 attaataggt cttttttata tagatacaga taagcataca tggcaaaatg gagattatca
    33781 aattgaacta gagcttaatt ttgagaatat aatggatgaa aaatctgctg gccaagatga
    33841 gcaaaaagaa gatggttcag agtatactgg aggaacagaa taccctgctg aatttacggc
    33901 ttattgtcct agaaaagaag agggtggaaa tacagattgt agaggtaaaa aactagatcc
    33961 ttctaaaaag acatgtgccg ctcctatggt tggagcatat gaaaaatctt actatacaaa
    34021 agatttctta aacaaacatc ctcttatgag atatggagat gaaatacaat tagttacagg
    34081 tgtttcaagc cgagatggaa cgtataaagt aaatgataat gggcctgcaa taattataga
    34141 aaaggatgga acatatcata tagatatttt atttggaaat gttgaagaag ctaataactt
    34201 tggaagaaga aaaggaaaga ttataattgg tggttattct ggaaacatga cagaaaaagc
    34261 aaaaacagta atttcagaag caagaaaaca tcttggaaag ccatacaaat ggggtgggaa
    34321 tggaccaagt agctttgatt gttctggact aatggtatat tgctttaaga aggttaatgt
    34381 taatttacca agaacctcaa gtcagcaatc taaggtaggt aagaaagtag agaaaaataa
    34441 tttacaggca ggagatttgg tatttttcca taatccagtc agccatgttg gactatatgt
    34501 aggtaatgga gaatttttac atgcgccaca aacaggagat gtagttaaaa taagtaaatt
    34561 aagtaataga aaagatttta atacagctag aagagttttg taaaagaggt gatatcgtgg
    34621 cagatccaat caatgaattt ataggaataa ttaggcaaga aggtaaattt cataatgaac
    34681 cttctttttt tattggaaaa attaaaagta aattaccaga tttaaaaata gagataaata
    34741 acatcatatt agaaaaagaa gatattttga tagatagttg gatgcttgat agacagatag
    34801 agttatttga tacagaaaca agtcaagaac ataaacatga aataaaaaat ccctttatag
    34861 atacttttga atctggaaac acagtaataa tgtttaaaat aggtgaaaaa tttgctgttg
    34921 taagtaaatt ggtgagtcta gatgaataat actatattcc ctttcatggg tgttccagaa
    34981 gattatattt ctccagacaa tgaagaatta cctattttta aagaatttgc ttgggatttt
    35041 aataaagatg aaaagataat tgaaaatgga gactttaaaa tagttgaaag aaatgaagca
    35101 attaaagtat ggatttacaa aacaataaaa acagataagt atgttcattt aatatatgat
    35161 tggagttatg gaacagatat aaaaaatcta atagggcaaa agtatactaa aggtcttact
    35221 gaaagtgagg caaaaaggta tatacaagaa gctctattaa ctaatccata tatattagaa
    35281 gtcaatataa caaatgcaga atttgaagat gataatttat ctataagttt aaatgtaaaa
    35341 acaatttatg gagaggagga gattactttt gtataatggc caatcattca gtaccttaaa
    35401 taatagaact ttaaataaca taaatctccc tctttataaa ggccaaggat cttctcttta
    35461 taatatagtt tctccaataa attcagaact tgcacaatta tatatagaac tttcttatat
    35521 acataagaga gtttttattc aagataattt tgacgatttt cttgatagaa gagtcaatga
    35581 atttggcgta tatagaaaat taggtacaga ggcaataggt gaggtaacat ttgagggcaa
    35641 aattggaact caaataccaa atggaacaat aatatctcat agtgatttac tatttgtaat
    35701 tattaaagat attacaatag atgaaaatag taaattaaat gtaagcccta tacaggcttt
    35761 agagattggc attaagtata atttatcagc taacacagaa tttaaactta tagaagaaat
    35821 aactggagta actaaaattt ataatgaact tgacctaaag ggtggaacag aaatagaaac
    35881 tgatgaagaa ttaaaagaaa ggttctataa aatacaaaaa aatcaagcga caagtggaaa
    35941 taaggcgcat taccaatctt gggctttaga ggttgaaggt gtttataatg caaaagtaat
    36001 tcctcgttgg gatggtccag gtacaattaa aattctaatt tatggtcaaa acaatcaatc
    36061 agttgacaat gaagtattgc aaagatgtac tgaacatata gaagaagaaa aacctattgg
    36121 acctactgtt acaattgtta caccaagtac ttttgatatt aatattagtg caactttaac
    36181 acttgaaaat ggatatgata ttgaatctat aaaagttgtg tttttagata ttataaattc
    36241 ttatttgata gaaaattcaa gagagattgt ttatataaaa gtaatgagtt tgttagcaag
    36301 catagaaggt attcatgata taaaaaatct ccttgtaaat ggtgatgtta aaaacataat
    36361 agttgatgaa gaaaaagttc ctgctgtttc aagtgctata tttgatatcg aggtgagtta
    36421 aattgaagtt aattgattac ctgccaagtt ttgcaaacaa tgaaattgac atacaaatcc
    36481 aagaagcact tgaaaatgaa cttttaacgc taattgatga aaaagatgat ttattagaac
    36541 agttttttat agatacagca acttggggac ttgatgattg ggagaacctt ttaagcataa
    36601 aagtaaatta taagttagac ttcgatacta gaagaagcaa tataaaagca aagatgagag
    36661 gtaagggtac aacaacaata gaggttataa aagctatctc ggaagcttac acaaagacca
    36721 atgttgatgt agaagtattt agtaatctat ttagttttac acttagtttt ataacaaata
    36781 attgtagtta taacactatt ttagaattag ataagaaaat agaagaaata aaacctgcac
    36841 accttgaaca caaattcgag aggatattat ttaataaaaa cgagctttat acaggtgcag
    36901 caattagtac aggagaaaca gttacaatat atccttatgt acctagaaat ttagaaagtt
    36961 ttggagaaat agctatttgt agtggaaatg atagagcatt agaaaaagta acattgtatc
    37021 ctaaaaaata gaaatgaggt gataaaattg acagaacaac aatattttac tctagtaact
    37081 gacattggta aggcagcaat agcaaatgca agtattacag gtgaaaaagt agattttgca
    37141 aagataaaag ttggagatgg aggagggagt tcttatactc caaatgagag tcagacagca
    37201 ctaaaaaatg tggtttggga aagtacactt gaacatgcac aaggagataa agataatcct
    37261 aactgggtag taatacaaaa attcatacct ggtgatgttg gaggatttga aataagagaa
    37321 gttggtctat ttgattctaa agaccaatta ttagcgattt ctagttaccc aacaacatat
    37381 aaacctactg cggattcagg gactgtaaaa gaactattaa taaaagtaat attagttgta
    37441 tctaatgtag ctaatattaa tttgaaagta gacccaaccg taattttagc aactttaaaa
    37501 gatatacaag aattagacac taaaatagat acaactaaaa cagaattaac aagcaacata
    37561 gaaactgcta aaacagagtt gaacactaaa ataggagata caacacaact tactacaaca
    37621 gataaaacaa atatcgttag tgcattaaat gaggtaaaaa ctagtgtaga tagtatagaa
    37681 acaacagcag ataaaacaag tataaaagat acagataatt tatttgagag cgacaacgta
    37741 gagggagcat taaaagaagt gatgcaagaa gtaaaaggta atagaagcag tattatatca
    37801 acaataaata gtaatttaat accaatgtag aaagtgaggt gataaagtga agatatggaa
    37861 atacaataga actgtgaaga aaagaagagg tgtctataca gaattagata tacttgcaca
    37921 aaggtatgaa tttgaagcag atgggaaact ttatacagct tattttcatt tatatactaa
    37981 taagaatata gatatattca gagataaaga tttatataaa gaatcttata tgatgagcta
    38041 tagagttgga aatgtaacaa ctgatattga tgatgtagta cagcaaacaa ctacagtaag
    38101 atttatacct gaccctgtta ttgatattgt gattgataac attagcagag gatgtgaaat
    38161 aaactacaga attatagata atgaaccaag tgtaaaattc attgttacag aaaagttaaa
    38221 tggcacaata atagaaacaa agacaaattc tagtgataat aattataaac ttaaattaac
    38281 tgatgaacaa atagtaccat tagcattcaa ttcccaaaat tcaatagtaa tagaaataag
    38341 tacagaagat ggtggattag taacaagtaa aacagtaaca tttacaagaa caaataacaa
    38401 gccaacaatt ttagttaatt catatagttc taattctgca aaatttacag catctgattt
    38461 agataataat ttaagtaaaa ttgagtggta tttagatgat gtattaaaag aaacaataac
    38521 aacagattta acagcagaaa aaaccatcaa ctatgaattt acagacaatg caatacacac
    38581 attaaaaata gttgctacag atgcagaaaa tgcaacagtt gagaaggttt taagtataag
    38641 taaagagata atgccacttc aatctgatgc tagtttaagt gatatatcaa ctaagctgat
    38701 agagattgga gaagggttta gaaatggtaa aacaagtatt ataaacactt tagcacttaa
    38761 aaatatagaa gcaagtttaa ataatacact tgtggagtta tcagagaaaa taaaaacaag
    38821 ttttgatagt tcagacgcta gtgtgcagga tttgcaaaat agaataacag aattgaataa
    38881 tcagcttagt cgaagaaaaa aatgggctac aggtagatac acatttacta gagaagaaga
    38941 tactatcaat ttcagaatgg ttgatggaga tagtctttcc gcagtaaaaa aactaattat
    39001 acccattaat ttagattttg ttccaagtgt tattataata gacaaaatgt ttttaggtag
    39061 ttacgtacct gcacaagatg tgcaatttaa ttgtgtttca aacttagccg attttacact
    39121 tggacgtcgt ttctataata aacaatggat ttgcacttct tctataaaac tagattcagt
    39181 tacacaaaat agttttactc tttctttaca aaaagttgtt ccatttgata aagttagtag
    39241 tacgattttt tctattatta atactggttt tgcttggaga gcatatgaat aaaaatataa
    39301 attagaggtg attaaatgaa tagaaataat agaataattt acgaccaaac aggcaagata
    39361 tggcttcaaa ctggtgaagc aataggagat atacaagagt tgtcagaaat aactgaatta
    39421 aatttcatag atgttgaatt tggtagtata gactatagta aacaatatat agagtctata
    39481 aatccagtta caaaagaatt aaatataaaa aatatagaag ttgttttgac tgatgaacaa
    39541 aagagattag aagtattaga aaaagaacta aatatgttaa aagaagaaaa taaaaataga
    39601 gattctgaga tagtaaatac agcatttgaa gtaggaaata tcaaattaaa caataattta
    39661 taggaggaaa ttatatgtat aacttattaa agttaatgat agaacaaaaa aactatagca
    39721 ctaaggagga tttacaacat aagatggatg tattctatgc agtaaatagg attacagaag
    39781 aacaatattt agagttaaca ggtttattaa ataaagaaga aacaccagta gaaccaacaa
    39841 tataggttct tttttattga aagaaggtga ctaaatgact tttaaagaat tagttagtaa
    39901 agttagaaat cttgtattag aagcaaagaa tgtaactata gaagatacag agaataaatt
    39961 cactagtgaa aatgtcgagg gagcattgaa agagtgtata gatagagcag atttggcttt
    40021 tcaaagtgcc gatagtggaa aagagttaat tgcaactgct atcggctctc ctgctacatc
    40081 cgaacaaaca tttcaagagt atgctaatta tattgcagga tttaaaaata ctataagtca
    40141 gttaaattct aaatataaat atactagtgg aacttttacc tattatactg gtgcatatcc
    40201 taatgggcta aatataggtt ttagaccaga tattatttcc tatactgcca ttaatgctgt
    40261 agaggaaaaa tattattgtg tatacacacc tttattctat cttttttata attatacaac
    40321 tggagaaaaa tatcttgatg ttaatcataa tcgttatgga ataactttta ctgacaatgg
    40381 atggatacct agaaatccag ttaacaatgg tgtgtatagt tatatagcag ctaagtgccg
    40441 ttagttgtca aagacttaga taaatttcta agtcttattt taatacaaaa ttaggaggaa
    40501 gtatgaacgt aacaatagtt tttttagcaa caaatatatt tataaaatta gtaatattag
    40561 caatagcatt tgatacattg ctaggttgtc taagagcaat aaaaacacat cagtttaata
    40621 gctcttttgg aataaatgga ggaataagaa aagttgcaat gatagcatgt atattttttc
    40681 tagcagtagt tgacatactt acaaagttta actttttatt tatgttgcca caagattggg
    40741 ttgatttctt acgactaaat catctaggaa tatctgaatt tttctctatt ttattcattc
    40801 tatatgaaag tgtaagtata ttaaaaaata tgtatttatg tggattacca gttcctaaac
    40861 gattaaaaga gaaaataggt agtttattag atactatgac agatgaatta aatgttaaag
    40921 gaggaaaata aataatgaaa atatgtatta cagtaggaca cagtatttta aaaagtggag
    40981 catgtacttc tgctgatgga gtagttaacg agtatcaata caataaatct cttgcaccag
    41041 tattagcaga tatatttaga aaagaagggc ataaggtaga tgtaataata tgcccagaaa
    41101 agcagtttaa aactaagagt gaggaaaagt cttataaaat acctagagtt aatgctggag
    41161 gatatgactt actcatagaa ctacatttaa atgcaagtga taggcaagga aaaggctcag
    41221 aggttctata ttacagtaat aaaggtctag agtatgcaac tagaatatgt aagaaactag
    41281 gtacagtatt taaaaataga ggagctaaat tagataaaag tttatatatt ttaaacagtt
    41341 ctaaacccac tgcaatacta attgaaagtt tcttttgtga taacaaggag gattatgaga
    41401 aggctaagaa acttggatat gaaggtatgg ctaagttaat tgtagaaggt gtattaaata
    41461 agagtattga aagtaatgag gtgaagcaaa tgtataaaca cacaatagtg tacagtggtg
    41521 atgatagagt atcagcagat attttaggat tatattataa gagagcaaaa gaaagttatc
    41581 tagttataga tataaaagac tataaaccac atagaacaca aaatctatat gtaattggtg
    41641 gagtaacttg taataaaatg aaagaaatga gtaagactac aggagaaaaa tttactcaac
    41701 tatatggtaa tgatgtatgg tcaacaatgg ataaagttat agaatttgta aaagaaaaat
    41761 tataaattaa aagacaacct ttaaaaatta attgttaata aaactaataa aattttcaac
    41821 tcagaaggag cattatatgt taacattttt agatttgttt gctggcattg gaggttttag
    41881 gctagcaatg gaaagagcag gacacaaatg cgtaggccat tgcgaatacg ataaatttgc
    41941 aaacttaagt tacaaagcaa tgcataaccc aaaggaggat gaatggtttg aaaaagacat
    42001 tagagaaatt agagcagaaa atattcccaa agcagatgtg tggtgttttg gattcccatg
    42061 tcaagacatt agcattgcag ggaacaaact tggattcaaa ggaaatcgtt caagtttatt
    42121 tttctcagtt acaaaactta ttagagaact taaagaagaa gatagaccca agtacttact
    42181 tattgagaac gttaaaaatc tacttagtgt taatagagga tttgacttcg ccaaacttct
    42241 cattgagttg gatgaaatcg gctacaatgc agaatggcaa aatctcaatt ctaaaaactt
    42301 cagagttccc caaaacagag acagaatata cattgttgga catcttagaa gaagaagtac
    42361 aagagaagta tttcctatca caggaacgag tagaaaaact actcttgagc aattaaataa
    42421 tcctaatcac agttcaaaca ggatttataa ttcaaatgga ttatcaaagt gtttattaag
    42481 taaaggtggg aacaatacag gtctttatag agttggcaat atcaatccta gtggaaatgg
    42541 tgcaggcgga caagtgtata gtagcgaagg cttttctccc acacttacaa ctggaaaagg
    42601 tgaaagccca aagatattaa ttaaagagaa tactaaaaaa ggatacatca aagcatctat
    42661 aggagatggg ataagactag atcatataga aggtaaaact ggccgtggaa gagttcagca
    42721 caaaaaaagt tgtactttga ctactgcaag taatgctggc gtaattggta aaaaatttag
    42781 agtaaggagg ttgactccaa aagaatgttt taggttacaa ggatttccag acgattatta
    42841 cgatagagcg gcaagtgtct gttcagaaac tcaactatac aagcaagcag gcaatagtgt
    42901 tactgtagat gttgttgaag aaatagcaaa gaaattaaaa tagaatatta atacaatgca
    42961 aaatgtggta ggtttttcct tccgcatttt gcattgcatt agcttttttg gaataactta
    43021 cctgtactat cttaaataaa aaaatgctac tatattataa acatttacaa ggagtgcata
    43081 atgttaatac aaagcttaaa acgtggcgaa ttaattgtaa tgaaaatctt ttagaaaaga
    43141 aattcaagaa tatcaaagaa agaaattatt cgcatttgca atgaaaaaca caattggcgg
    43201 aaatctgcga caaaaactct acttaaaaga cttgttaaaa aaagagttct acttaagaga
    43261 attaaaattt tccacatata ttacatacct atagttacag aaaaagaata tttcaaatat
    43321 aaaataaatg atttagagcc agattattta gatgaatatt ttatacgatt aatgtccact
    43381 gtacataaat ttaaaaatct agatgaagaa gattaaatat ctcccttcat ctactcttca
    43441 catattttaa tagactttca agcaataatt gacttaacaa atcatgctta tcataatgtt
    43501 tattctcatc tgcaaattta ttaaacattt cccacactgt attatttatt ctaactgtag
    43561 ttctttttat agattgatct ggaagatcta tagatatatc attttcttta atgacacata
    43621 tatgataact atcatcttta gttttaaacc attccagcac attctttata ttatcaattt
    43681 cactagataa atatttaata tcgtcatcaa tcttactgct aatgctatct atatgatatt
    43741 tatcatcata acttttatta tcttctacaa acttgtttcc agttctttta tatcctttct
    43801 tattaagcca cttccttata gtactttcat ttgcccctaa ttcatctcca acttttttaa
    43861 gactttcaaa ttcacttaat ttattattaa tacaatcaac aacttcatta atattttttt
    43921 ctttcaaatc actccaagtc atataaatcc tcctcaaaaa aataatagat tacaaatatc
    43981 atatatttgt aatctattat acatactata aactaaatcc ttttttattt catcattttt
    44041 ttgtatcctt ttgcatttgc aaaaacatgt ttatctacat cactcataac tattatgcca
    44101 ggtatattct ttttaaatgc ttcacctaac aatttagcgc ctccacccat aacaacaacc
    44161 gcagctgtac taactggata atttaatttc aattcattgt atatttcttt aaatagagaa
    44221 atagatgcat ctatattttt ctttatatct tgtttttcac catttaggta aaatccttta
    44281 tttatgtagt cttgtatttt ttctcttttt atctcaactt tacctaaaaa tctttcgtcc
    44341 atctcaagag atttctttat tgcatcatat atatttattg ttcccacatt tatagaatat
    44401 ggatcagtcg ctttattccc ttgtacaagc gcaatatctg ttgtctttcc accaatatca
    44461 actaaaacaa catctcttcc tgcaatttgg tcaactatgt cttttgcata atataagtat
    44521 cctgtaacac cttctggaaa tgtttgaata gatcttattg ttatatctct tttagtagtc
    44581 tcatttcctc ttgtaaactc aacatgatat tcttttcctt gtaatttttc aataagattt
    44641 tttcttaatc cggcaaattg tttcactggc agacctagcc ctagatcaac aatttcatca
    44701 tctgtatttc tacatatagc ccctaatagt agtggtaaga agttttcttt ctcatattta
    44761 actatattta tttcctcgtc accttcacct aggtaatatg ttttattgcc tattttaatg
    44821 ctatctgaat ctgaaccaaa gttttttacc tctgtaactt tactttcaaa aatatcatct
    44881 gtacttgttt taactgcata atttccaata tctataccta atttactcat ttatgttatt
    44941 tcccccttat tattctaaac ttccaaaatc aaaattgtta gctggatctt ctatttcttt
    45001 tttctgttca actacagcat tatccttttt ttcttgcatt ttttcccaaa caagctcttt
    45061 taagtaagct gtagctgata atttctcatc taaaaacttt tcaattgctt tttctttatc
    45121 attgttttta aatgttagaa ctatcctact cattcaacca cttccttttt tataaaatat
    45181 tataaaattt attaatattt ataaaatatt ttactcatat gtgaatttat tttttatttc
    45241 agtttaattt aaggtataca aaattatttt ataaaatcaa caccaaaagt catttttaca
    45301 acaattacag caacaatctt tacaaccaca atcattgcaa caacaattac tacagttgtt
    45361 acaacagcaa tcattacaac aacattgttt tatttctact tgcactcttg atatattatt
    45421 gttaaggttt gaatctggtg ttgttgaagt aatttctgca atatttgtta atctatcaag
    45481 acatgttgat tttataattc ctcttaatat aatcactctt aaagttccag ctggtagtgt
    45541 acctatatta agacttccag tccatggttg gaaacttaca cctctatcaa gtgaaaatac
    45601 tgctttctta agcttatctg aaacattgtc tattaaagta acattttgag cattttctgg
    45661 tcctgcattt gatactacta ttacaaaatc tatttgctct cctacacatg cttctgtttt
    45721 atttgctaat tttattacag aaatatctgc tgatgaactt atatctattt ctgatgttga
    45781 agtattatta tctggatttg ggtcaggtgt agttgaactt actgttgctg tatttataat
    45841 tgtacctatt gctgtttgac ttactattcc ccttattata atatttctta tctctccagc
    45901 atctaaagtg cctatactaa gacttccatt ccatggttga aaagtcactc cattatctat
    45961 tgaatattct ggacttaaaa tagaagctgg tatattatct gttaaagtta cgttctgagc
    46021 agtagacggt cctgcatttc tcacctcaat tgtatagtct agtacatctc ctggtacaac
    46081 aacagtctga ttgcttcgtt ttactacaga aatatctgct gatgaactta cctctgcttc
    46141 tgatgttgaa atattgttat ttagatttgg gtcaggtgta gttgaactta ctgttgctgt
    46201 atttataatt gtgcctattg ctgtttgact tactgttccc cttattatga tatttcttat
    46261 ctctccagca tctaaagtac ctatattaag gcttccattc catggttgga aagtcactcc
    46321 attatctatt gagtactctg gatttaaaat agaagctggt atattatctg ttaaagttac
    46381 gttctctgca aaagatggtc ctgcatttgc aatttcaatt ccataaataa atcttccccc
    46441 tggtatagca actggttcgt tgccttttat cacagaaata tctgctaatg gacttatatc
    46501 tgtttcatct gttgaagtat tattatctgg atttgggtca ggtgtagttg aacttattgt
    46561 tgctgtattt ataattgtac ctactgctgt ctggctcact gttcctctta ttataatact
    46621 tcttatttct ccagcatcca aagtgcctat actaagattt ccattccatg gttggaaagt
    46681 tactcctcca tccaatgaat actctggatt taaaataaca tctggtatat catctgtcaa
    46741 aacgatactt tgtgcagcag atggtcctaa atttgtaact gttatagtat aactgaaagt
    46801 ttctcctgga actgctgtat ccatattgct agtttttatt acttctatat ctgctgatgg
    46861 aactatagga gttgtcactg ttgatgaatc cgtaactggt tctggtacat ctgcacttac
    46921 ttctgctgta ttagtaatat ctcctgtagc agttgaactt actaatccct ttatgataat
    46981 tcttattaat tgccctggtg caactgtacc tatattaaga cttccagtcc atggttggaa
    47041 agttgctcct ccatccaatg aatattctgg atttaaaata acatctggta tatcatctgt
    47101 taaaactaca ttcgtagcat tagatggtcc ttcattaaaa atttcaattg tatatacaaa
    47161 ttcttctcct ggaacagcta catcctcgtc agctactttt ataatagaaa tacctgcttg
    47221 tgggttaact ggtgtaataa tggtagatat attgttgctt aaatctgggt caggtgtagt
    47281 tgaagaaact gttgctgtat ttactatata ttctgaagaa tttaaactaa ctgttccttg
    47341 aattaaaact gttaatgaaa ttccagagtt taaagttcct atattaagac ttccattcca
    47401 tggttgaaaa cttgctcctc catccaatga atattctgga tttataatag aagctggaat
    47461 atcatcattt aatattacat tttcagcatt tgctggtcct aagttttcta cttgaatagt
    47521 atatctcaat aaagtacctg gaacagctga attaggttct gctgttttca ttactaaaat
    47581 atctgctaat aaattcactt ctgtctctat tgtagatgta ttattattaa ggtttgggtc
    47641 tgctgtagtt gaagatacta cagctgtatt tgttataatt cctactgttg atggatttac
    47701 aataccccta attataataa ctcttgtctc tcctacttct aatgtcccta tatttaaact
    47761 tccagtccat ggttgaaaag ttactcctcc atctaatgaa tactctggat ttacaataga
    47821 agctggtaca tcatctgtta aaaccacatt ttcagatggg aatggtccat tatttactac
    47881 ttttatagtg tatattaatt cctctcctgg attcactata gtaggttcag ataattttgt
    47941 aacttcaata tctgctgaga ttacttcaac aggaactgga ttagaatcta ctgaaaaatc
    48001 atttggtata ccgccttcta caggtgtata ggagtaatct atagtagcta tattattagt
    48061 aggatttggg ttaggaatac tttcaaccac aacatcaaaa gttacttcaa cccctgttcc
    48121 tcctccaata tctggaagtg ggaaacctgt attggggtct actcctggta cagatactcc
    48181 atcaaccgtt acagttccag gtataaactc taaaccattt ggaattaaat ccctaaaaaa
    48241 tacattagtt aaagggctat cacaagtatt atctaatgta actgtatatg tgattatatc
    48301 acctacatta gcagtttctg tgttacttgt ttttattgga gtaaaagttg gaacagctat
    48361 ttctcttaaa gaaacatcat ctatagcata gtcatttcca atggcgcttt ctccttcact
    48421 aaaaaattct actgtaagtt gattattatt ttgagaatta atcacagtac ctacttcttt
    48481 ccattctggt gcgtttgtat taactggaat ttgagctcct aatgttgctt gatatagtat
    48541 attattattt tggtcaaata ttcttacacc tagctgtggt ggtggatatc ctgttacctt
    48601 aaaaaggttt aaaatccaag tgctaaataa ataattagtg ttaggtgtaa cttgtactgt
    48661 tgatttaaaa aatacagctc caggattaaa accatttacc accatcattc ttcctgtttc
    48721 atttcctgta gtatggtcag caatacgcca ccaagctcct ataacattac tcattgcatt
    48781 tgtcataata ttttgtactg tatattctcc atctaaaggt gtaaacttag ttgggtctgg
    48841 tacaacatac gtaaaatctg gcgtcacatt tgggtaaggt tctatttcag gtcctgtatt
    48901 acttggtgta cctggtggaa aaaaaccgaa tgttccgtta tctgcatctg taataaggtt
    48961 tatattagat acagatacgc aagaatccaa tatattctct ataggtgtat aaatcacagg
    49021 cccatttaat atattttgat ttgttatatc tgaacctgct actgttatta atagtgtgtt
    49081 aggtgttgta ccatctaagt ttgttgaacc aataggagga cttgtcacag aactaatagg
    49141 tggcatttct gcaattggaa ctggacctac tatcgctgtt gcaaaatttc ctggtgttgg
    49201 tactcctcct gtagttccaa aagcttctac aattctatag ttaccatttg gaacattagt
    49261 aaaaacataa tttcctgatg catctgtgag aaccacaagt ctaactccag ttgctatatc
    49321 ttgtaataca actggtatat tggctattcc tgagtctcca gaatcaatgg tagcacttct
    49381 atttctatca aaaactattt gtcctgatac tgttgccata ttatcactcc tttgtgatta
    49441 caaaatttgt gttcataata tattattctt taaaactctt ttatgtgaca ttttaagcct
    49501 ataagtgcgc tttttttcta taacatctaa tatgtatttt tttttatttg caatatcaat
    49561 ttttttatat tttattgtat atgttaaaaa atatcttatg tcataagtta aaaactatca
    49621 aataatagca aatggcataa aatgtattat tttatgccat ttttcaataa tcaagtattt
    49681 tcaatgtatt tataataaca aaattacaat ttgtcagtga aatgtcagtg atttttttgt
    49741 tattttttac tctttttaaa ttttgaagat taagagtatg cttttatatt ataaatcatc
    49801 aaacaaaata tcgtaaaatt taaacttaat tatttaagat atttagagat ataattttct
    49861 gtattttatt tttaatctaa ttgagtttag agggtctatt tttctcaaaa ataacaagct
    49921 tttatattgc tttttaaatg ggtttaattt ttttcggtga tagtttgtac ctttctaatc
    49981 aaatcaagct taaaatctac ttaaatgctt aatccggtac tttaatctaa aatcaataat
    50041 tttagttgac tttattttta ttatgtaaac taaaattttt ttctgaattt tatatataat
    50101 t
//
LOCUS       DAMJZE010000009        49118 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJZE010000009 DAMJZE010000000
VERSION     DAMJZE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.009"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            72..407
                     /locus_tag="PC541_000428"
     CDS             72..407
                     /locus_tag="PC541_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2837912.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            544..717
                     /locus_tag="PC541_000429"
                     /pseudo
     CDS             544..717
                     /locus_tag="PC541_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein CcsB"
     gene            992..4036
                     /locus_tag="PC541_000430"
     CDS             992..4036
                     /locus_tag="PC541_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2837913.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKQRVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDDNG
                     NYSVAGATYGVFADKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGRTATLKVSDTPKVTDTLIKLFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTYGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSKSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     FSNPNEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKKVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIDDEGQTITFKEKPEEPEKPKTPQTPEKPS
                     RPSDSPKTGDSTNVMAFIVMLLVSAGGLAGTYLYKRRKMKKS"
     gene            4275..4601
                     /locus_tag="PC541_000431"
     CDS             4275..4601
                     /locus_tag="PC541_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2837914.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVELVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVQEK"
     gene            4617..5000
                     /locus_tag="PC541_000432"
     CDS             4617..5000
                     /locus_tag="PC541_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2837915.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHTPQGQPKK"
     gene            5102..5539
                     /locus_tag="PC541_000433"
     CDS             5102..5539
                     /locus_tag="PC541_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837916.1"
                     /translation="MCMECYVDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPK
                     RGLQRWNFPFKSLEIAKLYLRTADYSTKKSCGIYELKSENGRLSYKIFADSEDLLLYL
                     KKNKGKTCKDMKPVFMIEEYREYANTQIRKLTSDEIQKYMSER"
     gene            5632..7026
                     /locus_tag="PC541_000434"
     CDS             5632..7026
                     /locus_tag="PC541_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2837917.1"
                     /translation="MRMILNKGHRIRASNKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDNFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYMEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNSDLADLGTVMPNVY
                     HIKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVNLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            7209..8405
                     /locus_tag="PC541_000435"
     CDS             7209..8405
                     /locus_tag="PC541_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2837918.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLNTLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGRYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            8418..8552
                     /locus_tag="PC541_000436"
     CDS             8418..8552
                     /locus_tag="PC541_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2837919.1"
                     /translation="MWILLKDFLLVSMGMGIGVVLMCILNVGKEADYEMKQLEESEDN
                     "
     gene            8553..8774
                     /locus_tag="PC541_000437"
     CDS             8553..8774
                     /locus_tag="PC541_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837920.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            8809..9450
                     /locus_tag="PC541_000438"
     CDS             8809..9450
                     /locus_tag="PC541_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2837921.1"
                     /translation="MWIYKILIYGGVILKKAMIYVHGKGGSYLETEQFKNICLDFDVF
                     GVDYNDYFPWIVKNKIMEVYDKISEKYDYIYVLANSIGAYFTMHTLQNFKIEKALFIS
                     PILDMEQLIVDMMSWANVSEKELSERKEIPTDFGETLSWEYLCFVRANPINWKTPTEI
                     LYAEHDNLTSRQTVDKFIDRHCANLTVMNDGEHWFHTDEQILFRDNWLRKVIQ"
     gene            9651..10277
                     /locus_tag="PC541_000439"
     CDS             9651..10277
                     /locus_tag="PC541_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2837922.1"
                     /translation="MKYIMLHGLGQSSESWSDTVKVFNDDFEVLCPNLSDWLSGKTPC
                     YDTLYRTLETYCEQFEEPLNICGLSLGGILAMQYAIEHPEKMNSLVLIGTQYEMPKYL
                     LKMQNLIFRIMPCSAFEKMGFQKTDFINLCKSMTELNFKHELKKISCSVLVVCGDKDK
                     INKKASLELQQQIENAEIAIIENAGHEVNTDNPTLLGQKLNVFFKQYV"
     gene            10366..10662
                     /locus_tag="PC541_000440"
     CDS             10366..10662
                     /locus_tag="PC541_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2837923.1"
                     /translation="MSDIFKDMQANVGCEYISDLPSYKRKVWQEMKRLNPANYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLTCR"
     gene            10581..11084
                     /locus_tag="PC541_000441"
     CDS             10581..11084
                     /locus_tag="PC541_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2837924.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGIGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPNCKSMIDVAKQKLMNDPTFKHLSEDCQAYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            11102..11605
                     /locus_tag="PC541_000442"
     CDS             11102..11605
                     /locus_tag="PC541_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2837925.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIGEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNSMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            11724..12122
                     /locus_tag="PC541_000443"
     CDS             11724..12122
                     /locus_tag="PC541_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2837926.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            12100..14550
                     /locus_tag="PC541_000444"
     CDS             12100..14550
                     /locus_tag="PC541_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2837927.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGNLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDLGEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            14550..16736
                     /locus_tag="PC541_000445"
     CDS             14550..16736
                     /locus_tag="PC541_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2837928.1"
                     /translation="MKERIKGAFTKKKIFHFLKMSLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLFMRHRARCMERRLAGAVTAGGVAGAVAGRTISNRKPTRNTATKDSRGNTTSSMGQR
                     AGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTVYAVYSAKEKAKSSVSDFKRGMVQE
                     QQSRQTGRLEKQEQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     KPSAEKMQEVKRPATATTSKASEPVKTNVIKERPLFSGASDKKATQSAQPVHRQNVEK
                     VVSQETRQNYTKDRRTKVQQTQTVQKNQQTTEKTPNLVMKKGQKKK"
     gene            16733..17740
                     /locus_tag="PC541_000446"
     CDS             16733..17740
                     /locus_tag="PC541_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2837929.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHAFNLA
                     ENFAREKSGGKKVIYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVSQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGILVSPTQMYHAGNPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            17756..18667
                     /locus_tag="PC541_000447"
     CDS             17756..18667
                     /locus_tag="PC541_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2837930.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKAVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIESRTQA
                     INGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWSIEQSGTDTFSATYEVDQQIKEGEK
                     TSNVKATYTVKVHIDADGDMVIVQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGDNVKVKVAVKF
                     LDNQAKATQVSQYELVLQKDSNWKIVG"
     gene            18812..19033
                     /locus_tag="PC541_000448"
     CDS             18812..19033
                     /locus_tag="PC541_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2837931.1"
                     /translation="MKKGQHIFGTVKVGERGQIVIPKEAREIFDINAGDTLLVLGDEE
                     QGIAIVKADVMKELAVKILKGLGNLCLDN"
     gene            19062..19679
                     /locus_tag="PC541_000449"
     CDS             19062..19679
                     /locus_tag="PC541_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDF2837932.1"
                     /translation="MKNSISLFLPLALLLDLTIPFFLAPFYKGYSHSMQVMSVLGNNK
                     SPLHFIYNLWLVLCGIILLMSGFKIYEIVSQKSKNIAVILFIVILIYAIGGCILSGIF
                     SVGETKSLNTIPEKIHGYGAVIGFMCLTFAPLLISLYWFRIKEIPLALFSMICFLLAF
                     IFFTIFVMADKPNYKDTIIAFEGLWQRLSLLFMYLPLGCLILLEK"
     gene            19696..19932
                     /locus_tag="PC541_000450"
     CDS             19696..19932
                     /locus_tag="PC541_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837933.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPAPEEYIYIITKKTVKGLKV"
     gene            19929..20901
                     /locus_tag="PC541_000451"
                     /pseudo
     CDS             19929..20901
                     /locus_tag="PC541_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
     gene            20914..21528
                     /locus_tag="PC541_000452"
     CDS             20914..21528
                     /locus_tag="PC541_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2837934.1"
                     /translation="MKKVYVYLLDDMAECEIGYILQAFSMEKLLKNGTKEFEVKTVGF
                     NKEPVLTLGGLTIVPDYSISEVDFNASTALLLPGSSTWGSKENQEILEKAQECLNKNI
                     LVGAICGATLALADYGILDKFKHTSNSLEYLNFFSRNYAGQSLYVCSNSVLDRNLVTA
                     NSAGSLEWTKNILNYLNVYSDNKITAFYNYYSTGNAKYYDELLK"
     gene            21556..21729
                     /locus_tag="PC541_000453"
     CDS             21556..21729
                     /locus_tag="PC541_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2837935.1"
                     /translation="MGIMPICNNKTRTKIRVDTELISFPLFCPKCKHETLINIKHGNV
                     TTIKEPDAKTQSR"
     gene            complement(21961..22212)
                     /locus_tag="PC541_000454"
     CDS             complement(21961..22212)
                     /locus_tag="PC541_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2837936.1"
                     /translation="MKLFIGDIIQKLRKIKGLTQEQVANALGITTSSVSKWETNNTYP
                     DISLLSPLARLLGTAVDNVFNFKQKLSEEEIDTLLNRSK"
     gene            22477..23181
                     /locus_tag="PC541_000455"
     CDS             22477..23181
                     /locus_tag="PC541_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2837937.1"
                     /translation="MMKAILKTNNLCKDFKKQKAVKNVSITVRENSIYGLLGPNGAGK
                     STTLKMITGMLRPTSGKVLFNGHEWNRKDLEQIGALIETPPLYENLSAVENLEVRAKL
                     LNIPKTRIDKVLKMVDLQNTGRKKAGQFSMGMKQRLGIAIALLNSPKLLILDEPTNGL
                     DPIGIQELRSLIRSFPSKGITVILSSHILSEVQLIADDIGIISNGILGYEGQMNKDEN
                     LENLFVEVVRKSQEER"
     gene            23183..23926
                     /locus_tag="PC541_000456"
     CDS             23183..23926
                     /locus_tag="PC541_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2837938.1"
                     /translation="MKNYISSEYLKHKNTFAKKLVWLAPILTLCLNILAPMWYQQNSY
                     NWWYVFLYPGCLTLLSILIIQRDNGKLKFRAILSLPVDLKKVWYAKIITCIIYIVLAN
                     TILMLCNVAGGFIIQHIFDIPMTISPMQALFGTLCIILASIWNIPLCLWFTKKVGVFA
                     TLILNVGMSFMLGTLGANTSFWFICPYSWVTRLMVPTLGILPNGESVTGSALSTPLPL
                     VIMTICLSLLLLYTISKSTAKSFIKQEVL"
     gene            23926..24669
                     /locus_tag="PC541_000457"
     CDS             23926..24669
                     /locus_tag="PC541_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2837939.1"
                     /translation="MFSIIRCFQAEWLKSRHTPLLLVHVLFPLIGAGVFAGYFHISGW
                     SEMTNVTTFLEVLAIIFPFVIGIVVGMIVELENGAGHFQLILGTIRSRIAVYIGKLVY
                     LIVLATFATILCIFLFAVLYPVMPFSFYIKPLIMLILSVIPLYLISIPVGFSLGKSVS
                     MGIGIVGSLLSALLVTGLGDFIWKFLPWGWGVRFMDYCVLRYVNEGQFHSNLPEFQLG
                     FILMLVFTVVLFIISLVWFQRWEGVKENE"
     gene            24662..25471
                     /locus_tag="PC541_000458"
     CDS             24662..25471
                     /locus_tag="PC541_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HDF2837940.1"
                     /translation="MSKKMMILLFAVIICLSLAGCSLQDKIGDYSREQCYLNKENVTQ
                     FSYKGNDYTILADTVSNSGLGEWIGYIRQLAAVDESGKILLQENLKTATFQTLADLAD
                     LVDKAPNDAYIIPFLNVYAAPNADDYLIVDINGGYHKAVIDENIKGTDTVFDFKDIEQ
                     SMSGKFEINPQNATQLLCDGTVYQVTSDTVSNNELGSYIGILAENVIFNAETKIPLSK
                     EELRKIDWYGENAGQHREQWIYKDIYEIHGTEKTEAVAVQINDRYYIAKRQ"
     gene            25534..26193
                     /locus_tag="PC541_000459"
     CDS             25534..26193
                     /locus_tag="PC541_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2837941.1"
                     /translation="MATILMIDDERPILELVKNGLQKDGHLVTVYTSAAQVPLDKLNR
                     YDLIILDIMMPDIDGFSYCDKIRSLVDCPILFLTAKTMENDITFGLGLGADDYLTKPF
                     RITELRARVNAHLRREHRERHIALTFDRIKIDLSAKELRVDNIPVPLTKSEYLICEYL
                     ASNKGQVFSKEQIYEATFSLYGESDSSTISTHIKNIRSKFEKTGVQPIVTVWGIGYRW
                     E"
     gene            26184..27548
                     /locus_tag="PC541_000460"
     CDS             26184..27548
                     /locus_tag="PC541_000460"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012722.1,HMM:NF014567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2837942.1"
                     /translation="MGIKMKTSLTRTFYKFLVCLLGGLCLSVVVPFACMMFASSIGLV
                     TLADNNERQTKALVPILTATPNVSDIHFPTGTKFLRLTKNYGYIDTNMSEEEKEKALM
                     FAKTGFMDTSGKTQFIFITRDDEYIVLQYVIGSQYLNAQFNQYLPSPEIMMISLITIS
                     SLGVCVYLTVHFAKELRKELTPILEATKEIENNNLDFTIGHSKILEFENVIISFDSMR
                     NSLKGSLEKQWRSEKAQREQIASLAHDLKTPLTVMQGNIDLLDETELDEEQKLYLSYA
                     MSSSEQMKQYIKILIDISKASAGYQLQMEDVNFPEFWKHVLSQTEIICKDKGIVIQQT
                     QHNFPQNITGDRMLLERALMNLVSNSLEYSPENSTLYIDVDSKDNYLNICITDCGCGF
                     SQEALDHAKERFYMADQSRSSKLHYGMGLYIVDIIIKQHKGKLLLDNSTETKGAKVTI
                     KLPL"
     gene            27910..28329
                     /locus_tag="PC541_000461"
     CDS             27910..28329
                     /locus_tag="PC541_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2837943.1"
                     /translation="MKLSDFQKTIQCQFECKLKKVTKGIVRNYQKELKRRKNKEIPFC
                     ELPELVIEKLSVLDDYDSDYTLFNVCGNDISILDDELAEALKKLSDRNRENLLMYYFL
                     EMSDTEIAKLQNISRSGVFQNRYNSLELMKKILKGEK"
     gene            28333..28446
                     /locus_tag="PC541_000462"
     CDS             28333..28446
                     /locus_tag="PC541_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2837944.1"
                     /translation="MKHIFKKPSFYLISSATDGDEKAIEKILAIYDPYISK"
     gene            complement(28491..29396)
                     /locus_tag="PC541_000463"
     CDS             complement(28491..29396)
                     /locus_tag="PC541_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2837945.1"
                     /translation="MFIAIKTDDGRIKGKISFYCKVLHVSRQAFNKFLKTKDIPWKYQ
                     ALADAMLDICSEDECNDTYGRIRMYQALQLKQPEGVHIPGERTVYRVMAEIGLNHKPK
                     RKPNGITKADREASKSDDLIKRDFAAEKPLEKCMTDMTEIKASDGKLYVSAIFDCYDL
                     AVLGLAMDTNMRATLCEQTLENACKAYPMLRGAILHSDRGTQYTSELYRKAINRYGIV
                     QSMNSAGGRCHDNARCESMWARFKEELLYGRYDPTSMTVEQLKTLIWRYFISYWNNRR
                     ICSANGGLPPMVKRQQYYASLQEAA"
     gene            complement(29396..29737)
                     /locus_tag="PC541_000464"
     CDS             complement(29396..29737)
                     /locus_tag="PC541_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2837946.1"
                     /translation="MAQNQKSYDNEFKAQAVKLAQEIGGHKAAQELGIPKGTMYTWIK
                     AFKEGRLSANEAVHTPKNALSLNDELIELRKRVKEQDKEIRRLKEENEFLEEASAFFA
                     ASRRKSAKNRD"
     gene            29823..29963
                     /locus_tag="PC541_000465"
                     /pseudo
     CDS             29823..29963
                     /locus_tag="PC541_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            30339..30542
                     /locus_tag="PC541_000466"
     CDS             30339..30542
                     /locus_tag="PC541_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2837947.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            30621..31811
                     /locus_tag="PC541_000467"
     CDS             30621..31811
                     /locus_tag="PC541_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2837948.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYEKKNSQRKNIKRA
                     TEKGRQYLMNALKNDLLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEKHLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEIGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            31992..33107
                     /locus_tag="PC541_000468"
                     /pseudo
     CDS             31992..33107
                     /locus_tag="PC541_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
     gene            33228..34616
                     /locus_tag="PC541_000469"
     CDS             33228..34616
                     /locus_tag="PC541_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2837949.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            34987..35700
                     /locus_tag="PC541_000470"
     CDS             34987..35700
                     /locus_tag="PC541_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2837950.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKSKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            35818..36687
                     /locus_tag="PC541_000471"
     CDS             35818..36687
                     /locus_tag="PC541_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2837951.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(36842..37987)
                     /locus_tag="PC541_000472"
     CDS             complement(36842..37987)
                     /locus_tag="PC541_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2837952.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(38320..39096)
                     /locus_tag="PC541_000473"
     CDS             complement(38320..39096)
                     /locus_tag="PC541_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2837953.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            39678..40652
                     /locus_tag="PC541_000474"
     CDS             39678..40652
                     /locus_tag="PC541_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2837954.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            40750..41055
                     /locus_tag="PC541_000475"
     CDS             40750..41055
                     /locus_tag="PC541_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2837955.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            41211..42371
                     /locus_tag="PC541_000476"
     CDS             41211..42371
                     /locus_tag="PC541_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2837956.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            42421..43323
                     /locus_tag="PC541_000477"
     CDS             42421..43323
                     /locus_tag="PC541_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2837957.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            43403..44296
                     /locus_tag="PC541_000478"
     CDS             43403..44296
                     /locus_tag="PC541_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2837958.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      44349..44530
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            44758..45888
                     /locus_tag="PC541_000479"
     CDS             44758..45888
                     /locus_tag="PC541_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2837959.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            45960..46985
                     /locus_tag="PC541_000480"
     CDS             45960..46985
                     /locus_tag="PC541_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2837960.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            46988..48184
                     /locus_tag="PC541_000481"
     CDS             46988..48184
                     /locus_tag="PC541_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2837961.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(48312..49037)
                     /locus_tag="PC541_000482"
     CDS             complement(48312..49037)
                     /locus_tag="PC541_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2837962.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT        17127 a         7461 c         9797 g        14733 t
ORIGIN      
        1 ttataatatt aataaatata ttgctataaa agtttgttta ataaattaaa taaagaaaag
       61 aggagaataa aatgtacaac atagatgata tgatatataa ttgtccagta gaagctttat
      121 caaatatttt aggtaaaaaa tgggtggcta taattatatg gaaaatacaa gaagataaaa
      181 aacgatttgg agagctacaa agagctatac cagactgtag taagaaaatg ttagttcaac
      241 aactagattt tttaattgaa caggggatta tagtcaacca aaaaaagttg gttaataatg
      301 ttgtagagtc aacatatttt ttaagtgaat caggattacg attgcttcct gtcatggaaa
      361 aaatgattat gtggagcaat aaaaatttaa attgtgataa atactaaaat ttactctgta
      421 agtaatgaaa atttaattaa gctatgagta gaaaaatgta ttaaaattac ataatttttt
      481 agttagattt taatttagaa atgggattgt aatattggaa ataaaataag gaggaatttt
      541 aatttgcaaa atgtaaattt atttttagta tttatagaag gtatagtatc attcttttca
      601 ccatgtatac tgccaatttt gcctatttat ttgagtatat tgtcaaatag tagtgtagaa
      661 aatttaaaag aaggaaagac gagttttata ggaagttctt tatttaaaaa cacaataaaa
      721 atctgattat ccgctaaaac acagtatcta tcagcatttt caagggaaca aatacatcaa
      781 tttactactt atttactacc attgaatgag ccttgatacg ccagttttcc tagatatgca
      841 aagatatggt acagcacatc agtattgaaa caagaaaaaa attgaatatt ttatagacta
      901 tggaacgcct aaaatgacgt tccttttcta ttttcagccg ttcttattgg acggctattt
      961 tattacccaa aatgaaagga gcattagatt tatgaaaaag atgttaaaac gattgtgtac
     1021 gggcttctta gctcttgcaa ctgtcgttac tgctttaccg actacacccg ttcatgcaga
     1081 aagtaagcaa tactggacgg agtcaaaaca gcgtgtcggt atcgttgaaa aagtaatgaa
     1141 tgacggttcg attggttcta cctttaatga gggacatttg accgttgagg gcgaggacgc
     1201 ttactgtatc gacattaaca cagactttaa gaatggctac aagaccagag ctgacgcaag
     1261 cacccgtatg agtgccgacc agataagcga cgttgcctta tctatcgaat atgtaaaaca
     1321 gtacacagac agccacagcg gaataagcaa aaatcacgct taccttttaa gacagctggt
     1381 tgtctggcaa agattgagcg tacatcttgg ctggcaatgt gataatgtgc gagcttctta
     1441 tgatgaaatt ccaaaggcaa cgcaggacga agttttctct ggtgcaaaag cctttgtcaa
     1501 agagaataaa ggacgctatg aatgtggcgg ttatatctac tctggcgagg ggcaggaatt
     1561 aggacaattc tgggcgaagc tgaatgttgg aaatgcaaaa ctgcaaaaga cttccagtaa
     1621 taccagcatt acagacgata acgggaatta ctctgttgct ggtgcaacat acggtgtctt
     1681 tgctgataag gactgcacga aacagcttgc cacccttacg actgatgaaa acggaaatac
     1741 agatgttgta gaggtaaaag caggcacagt ttatatcaag gaattatccg caccagcagg
     1801 atataaagta gataaaactg tatattcctt aaaggttgaa gctggaagga cagcgacctt
     1861 gaaagtatca gatacaccga aagtaacaga cactttgatt aagcttttca agattgatat
     1921 ggaaacacag aaagacaatc cgcagggaaa cgcttctcta gcaggtgcag aatttacatg
     1981 gaagtattat gctggcttct ataacaaaga caatctccct gccgaagcta ctcgtacatg
     2041 ggttacaaag acaatcgccg aaacagacag cgacggtaca acccactaca tcacaaaatt
     2101 agcggacgct tacaaggtat ctggcgacag cttctatatg caggacggca aagcggttct
     2161 tcctttagga acactatctg ttgaggaaac aaaagctcca aacggctact tgctggacgg
     2221 tgcatatatg caggctggcg ataagtctga acagataaaa ggcttatacc ttacacaaat
     2281 tacagaggac ggcgaccttg ccgtacttac tggaagtaac cagttttccg tatcagacaa
     2341 ggttatccgt ggcggtgtca aaattcagaa acgagattta gaaacgggcg atacaaagcc
     2401 acaaggaagt gctactttga aagatactgc ctttgacatc atttccttaa atgataatgc
     2461 ggtattggtt gagggcaagc tatacaagaa aaatgaagtg gtaaaaacaa tccatacaga
     2521 cattgagggt gtcgcttcta cttccgctga ccttttacct tatggaaaat tccgtattgt
     2581 tgagagtgaa gctcccgacg gttacttaac ttacggtgca aaaccgattg attttacaat
     2641 cacagaaaat ggaaaaatcg tggacttaac cgacaaagcc cattctatct ataatcagat
     2701 taagcgtgga gatattgagg gcgtaaaaat cggtgcaggc acacacaagc gtcttgctga
     2761 tgttccgttt cggatcacaa gcaagacgac gggcgaaaat catgtagtgg taactgatga
     2821 taacgggcaa ttctccactt ctgctgactg ggcttctcat aagcacaata ccaacgcagg
     2881 aaagaccagc gaggacggtg tgtggtttgg aacttctgaa ccagacgaca gcaagggtgc
     2941 gttaccttat gatacttaca tcattgaaga attacgctct aaaagcaaca agggctttga
     3001 gcttatccca ccttttgaaa tcgtggtatc aagaaataac cttgtgattg atttaggaac
     3061 gctgactgat gaatacgaaa aagaaatctc tatccatacc acagcgacca gcaaagacgg
     3121 cgaaaagact atccttgcag gaaaagaggt tacaatcgtt gacacggtca aattagacgg
     3181 acttacaaaa ggcacaaagt atcagctcaa agggtggcag atgttaaagg aagaaaatgc
     3241 cgagcttatc attgacggga aacgtgtgga aaacgattat acctttgtcg ctgatgatga
     3301 agaaatgaaa gtggaaattt cctatacatt caatgcgtct gctttaggtg gcaaaaatct
     3361 tgttaccttt gaagaattgt atgatttcag caatccaaac gaacccgtaa aagttgcgga
     3421 acataaagac attgaggacg acgggcagac ggtacttatc acagagcgta tcatcaaaat
     3481 tcatacaact gctaccgata aggacggcaa caaagagctt gaagcaggaa aaaaggtaac
     3541 aatcattgat accgtaacct tagagggctt agaagtcggt acacagtaca aacttgtggg
     3601 ctggcagatg ttgaaagaag aaaatgcaga gcttcttatc aatggaaagc gtgtggaaag
     3661 tgattatacg tttactgctg acagcgaaac tatgaaagtg gaagttgcct ttacctttga
     3721 cgctacttct cttgacggca aacagcttgt aacttttgag gaattgtacg atttgagcaa
     3781 tccagacgaa ccgaagaaag ttaccgagca taaggacatt gacgacgagg gacagacgat
     3841 tacctttaag gaaaagccag aagaaccaga gaaacccaaa acaccgcaga caccagaaaa
     3901 gcctagcaga ccgagcgaca gtcccaaaac gggcgacagt acaaatgtaa tggcttttat
     3961 cgtgatgttg cttgtgtctg ctggtgggct ggctggaaca tatctttaca aacgccgtaa
     4021 aatgaagaaa tcataaggcg gtaacggtat gatgaaagaa aaatcacgtt ctccgccgaa
     4081 gctgtcaaac ggcagatttc cgcttattct ggcttgtctg ccaacaacct gcaagaacac
     4141 ggatagtcaa gggtgcgtaa gcattgattt taccctttac tatctggggg attgctggta
     4201 cttcccaaac cgccagaata agaaatcaca atcaaaaaac ttatcaaaaa tagaaagaaa
     4261 cgaggtaaaa caatatggaa atgaaatatg tcgtgccaga tatggctcag tcttttggaa
     4321 cacttgaatt tgcaggcgaa agcgagccag tctttgaaag agataaaaat aaccgcaggg
     4381 tcttagccag acgaagctat aacctttatt ctgacgtaca gaaaggcgaa aatgttgtgg
     4441 tagaaattcc cgtgcaggct ggcgaaaagc atttcaagta tgaacagaaa gtagaacttg
     4501 tcaatccgaa gttatacggc agagggtacg caatcgggga tgtgggacat accgattatg
     4561 tattagtagc tgatgatatt gtagcagttc aagaaaagta aaggagtgaa aaaattatga
     4621 gattatcaaa cgggtttgtt attgacaaag agaaaacatt tggagaatta aaatttacag
     4681 cggtgcgtga tgtgttctta cagaatgagg acgggacacc gagtacccag cttaaaaaac
     4741 gtatctatga tttaaaatgt agtctgcatg gtggaattat ccccgtgtct gtaccgccag
     4801 aagtaccgtt aagggaattt ccatacaatg cagttgtgga gcttgtcaat cccgtagccg
     4861 atacggtatc aagaaagacc tatacgggtg cagatgtgga ttggtatgta aaagcagagg
     4921 acattgtatt gaagaataaa ggcaatcaga acgcaggcaa tccacagaac catacaccgc
     4981 agggacaacc gaaaaaataa acttggtggg tagattttta ttgtagcaga tatatggaac
     5041 tgttataata tgggtatgaa gaaagtaaca catttgaagt tgcaaaagga gtgaaattga
     5101 aatgtgtatg gaatgttatg ttgacgaaaa tagaataaca ccactcttaa atccattgga
     5161 ttgtttagaa aatcatacac aatacatttg tgggacttgc gggaggtgca tttgcattga
     5221 acatgacccc aaacgtggat tacaacggtg gaatttccct tttaaatcgt tagaaattgc
     5281 aaaattatat ttgcgtactg ctgattatag taccaaaaag tcatgtggta tttatgaact
     5341 gaaaagtgaa aatggcagac tttcatacaa aatctttgcg gacagtgagg acttgctgtt
     5401 atatctaaaa aagaacaaag gaaaaacttg caaagatatg aaacctgttt ttatgattga
     5461 agaatacaga gaatatgcaa atacgcagat aagaaaattg acctctgatg aaatacagaa
     5521 atatatgtca gagcgataac ttccagtttc acaatttcat atctatacat acatagcagt
     5581 tagtcttagg attgactgct tttttcgtac ccagaaagga gtgattgatt tatgagaatg
     5641 attttaaaca aaggacaccg tatcagagcc agcaacaaga accttgtcta ccacttttcc
     5701 atagggacgc ttctgtttgt atttgtggcg gttcttctgc tactgaatat caaacagctc
     5761 atgcgtaccg attgggagca tttcagcttg ttagagaacg gcttgacgct ctccccttac
     5821 aacttcataa ccatactgat agcgactggt gtttgtgcat tggtcgcttt tctgtattac
     5881 cgcttctgtt atgacaattt caagaaactc ctgcaccgac aaaagctggc acgaatgata
     5941 ctggaaaata agtggtatga agccgatact gtacaagaca gcggtttttt tactgacctg
     6001 caaagcagat caagggaaaa aatcgtctgg tttccaaaga tttattatca aatggaaaag
     6061 ggactgcttc atatccgctg tgaaatcaca ttaggaaaat atcaagacca gcttttacgg
     6121 ttagaggata aattggaaag tggcttatat tgcgagctga ctgacaaaac cttacatgac
     6181 ggctatatgg aatataccct gctttatgat atgatagcga accgtattac cattgatgaa
     6241 gtacgggcag aaaacggctg tcttagactg atgaaaaatc ttgtctggga atatgacgca
     6301 ctccctcacg ctctgattgc tggtgggact ggtggcggta aaacatattt tctactgacg
     6361 ctcattgaag ctttactgca taccaacgct gtcctttata tcttagaccc aaagaacagc
     6421 gaccttgcag acttgggaac agtcatgccg aacgtctacc atatcaaaga agaaatgata
     6481 gactgtgtca atgcttttta tgagggcatg gtacagcgaa gcgagcaaat gaaacgacac
     6541 ccgaactata agacgggcga aaactatgct tatttgggac ttccaccctg ctttcttatc
     6601 tttgatgaat atgtggcatt ttttgaaatg ctggggacaa aagaaagcgt aaacctactt
     6661 agccagttaa agaaaatcgt tatgttggga cgacaagcag gttatttcct tatcgttgcc
     6721 tgccagcgtc cagacgcaaa gtatttctcg gacggtatca gagataactt caatttccgt
     6781 gtgggattgg ggcgtatcag cgaattaggt tacggtatgc tttttggttc agatgtgaaa
     6841 aagcagtttt tccagaaacg tatcaagggg cgtggctatt gtgatgtggg aacaagcgtg
     6901 ataagcgaat tttacacgcc tttagtcccg aaaggacatg attttttgca gactatcggc
     6961 tctcttgcac aagcaaggca ggacgggacg gcgacgtgcg aagcgaaagg cgacggcacg
     7021 gactagccgt tgctggtgtg gcgaagccac gccagcaggt aaaacccctc ggtatctaac
     7081 agaggggtac gtttgcaaat agacaataga cgaaaaacat aggaaacata aggcttctgg
     7141 catggttttc tagcaaaatt cagttgtgaa gtcgcctaaa aaaacttcac actttacaga
     7201 ttgggggtat ggttctgaat gaagaacaat ggataaaaga attacgggag aaacggattg
     7261 cttatggtat ctcacaaggc aggctggcgg tggcttctgg tatcacaagg gaatatctca
     7321 acaagataga aagcggaaaa atgaagccgt caaaggaact tcttaatact ttgcataagg
     7381 aactggcaag gtttaatcca gaagcaccgc ttaccatgct gtttgattat gtaaaaattc
     7441 gttttcccac gctggatata cagcacatca tcaaagatat attaaaactg aatatcaatt
     7501 atatgctcca tgaagattac gggcgttaca gctatacgga gcattattct ttaggggata
     7561 tctttatcta tacgtcggct gacgaagaaa aaggtgtcct tttagagtta aaggggcgtg
     7621 gttgccgaca gtttgaaagt tacctgctgg cacagcaaag aagctggtat gacttcctca
     7681 tggacgcact catagacggt ggcgtgatga agcgtatcga cctagctatc aacgaccata
     7741 cgggcatttt ggatattcca gagcttgcgg aaaaatgcag gaaacgggaa tatatcggaa
     7801 agtccagaag ctataagttt taccagtcgg gcgagcttat caagcacaga gaggacgaca
     7861 gagaatatat gggacgtacc ctttatcttg gttctctgaa atcagatgtg tatttctgta
     7921 tttatgaaaa ggactatgag cagtacgtca agttagggac acctctggaa gaagccgaca
     7981 ttatcaatcg ttttgagata cgacttagaa atgaacgtgc ctattatgca gtacgagatt
     8041 tgctgaccta ttatgacgca gaacagaccg ccttttctat catcaaccag tatgttcggt
     8101 ttgttgatga agaaccagac aagcgaaaaa atgactggaa actcaatgac cgctgggctt
     8161 ggtttatcgg cgataacaga cagagcttga agctaacgac aaagccagag ccttacacct
     8221 tagaccgtac attgcggtgg gtacaacggc aggtagcacc gaccttgaaa atgctgaaaa
     8281 agattgataa gggaaacggt acagactaca tggaaacaat cgaacagcag gcaaagctca
     8341 cagaaaaaca tgaaatgata atcaaacagc agacgacccc tgcaaaagat ttagtagaaa
     8401 gttaggagtg atgaaaaatg tggatattat taaaagactt cctgctggta tctatgggaa
     8461 tgggtatcgg tgtggtcttg atgtgtattt tgaatgtcgg caaagaagct gactatgaaa
     8521 tgaaacaatt agaagaaagc gaggacaatt aaatgaattt cggacaaaat ttgtatcaat
     8581 ggtttttatc aaacgcacag agcttagtgc ttatggcgat tgtggttatc ggtatctact
     8641 tagggttcaa gcgtgagttt tccaaactta tcggtttctt ggtagttgcc ttgattgctg
     8701 tcggcttggt attcaatgct ggcggtgtga aagatgtact gttagagctg ttcaataaga
     8761 ttatcggtgc ataaaatttt attgtggtgc tgatatgaga atggtataat gtggatatat
     8821 aaaattctga tttatggggg cgttattttg aagaaagcaa tgatatatgt tcatggtaaa
     8881 ggtggaagtt atcttgaaac tgaacagttc aaaaatattt gtttagattt tgatgttttt
     8941 ggagtagatt ataatgacta tttcccgtgg attgtaaaaa ataaaattat ggaagtctac
     9001 gataaaatat ctgaaaaata tgactacata tatgtacttg ctaatagtat cggagcttat
     9061 tttacaatgc acacattaca gaatttcaag attgaaaaag cattattcat atcgcctata
     9121 cttgatatgg aacaacttat agtagatatg atgagctggg cgaatgtgtc tgaaaaggaa
     9181 ctttctgaaa gaaaagaaat accaactgat tttggagaaa cattatcgtg ggaatatcta
     9241 tgttttgttc gggcaaaccc gataaattgg aaaacgccta ctgaaatttt gtatgctgaa
     9301 catgataatc ttacatcacg tcaaacggta gataaattta ttgatagaca ttgtgcaaat
     9361 cttaccgtta tgaatgacgg agaacattgg tttcatactg acgaacagat tttatttaga
     9421 gataattggc taagaaaagt tatacaatag aatttagatt ttatatccac acacaaagca
     9481 gttagtttta ggattgactg cttttttctt atcaaaattt cagattggag tgatgaaatg
     9541 gtgtctgtta gagctgttca ataagattat cggtgcatag aattttattg tggtgctgat
     9601 atgagaatgt tataatgtgg atatgaaaca cagtatctag gagtgatgaa atgaaatata
     9661 taatgcttca tggtttggga caatcttctg aaagttggag tgataccgta aaagtattca
     9721 atgatgattt tgaagtttta tgccctaatt tatcagattg gctttctggg aaaacaccat
     9781 gttatgatac attgtatcgg acattggaaa catactgtga acagtttgaa gaaccgctaa
     9841 atatttgtgg actttcttta ggtggtattc ttgctatgca gtatgcgata gaacacccag
     9901 aaaaaatgaa ctcattagtg ttaataggca cacaatatga aatgccaaag tatctgttaa
     9961 aaatgcaaaa tctgatattc cgtattatgc cttgttcagc atttgaaaaa atgggctttc
    10021 agaaaacaga ttttatcaat ctatgcaaat ctatgacaga acttaatttt aaacatgaac
    10081 tgaaaaaaat atcatgttcg gtacttgttg tctgtggcga taaggataag ataaataaaa
    10141 aagcttcttt agaattgcaa caacagatag aaaacgcaga aatagctatt attgaaaatg
    10201 caggacatga agtaaataca gataacccaa cactactagg gcaaaagtta aatgtatttt
    10261 tcaaacaata tgtctgataa acaatttcat atccatacac acaaagcagt tagtcttagg
    10321 attgactgct tttttcttac caaaatttca gattggagtg atgaaatgag cgatattttt
    10381 aaggatatgc aggcaaacgt cggctgtgaa tacatttccg acctgccctc ttacaagcgt
    10441 aaggtatggc aggaaatgaa acgactgaac cctgccaatt atgaagaaag acagttagaa
    10501 gatttttcaa agtatgtgtt tggtatgtcg taccagacct taaaagatgt gatgaaacaa
    10561 cagaaaggac gtgaggaaca atgcaggaaa caagggtgct ggtggaaacg agaggaacaa
    10621 ctggcgaaga aacaatatca tactggtttg acctgccgat agatgttgcc gaatttgaag
    10681 aaaagttagg tatcggtgca gaaagtggag attaccgtat tatcgaaaag gtgttgcctt
    10741 atgctgatga agtccacgaa catacaagcg tgtaccagct caacgaatta gattttatgt
    10801 accgccagct ttcaagtgat atgcaggaag aatatgtatc actacttacg gtgtttgaaa
    10861 atttagaagc actttatatt tgcaggaatg tgattacggt ttatcccaac tgcaaaagca
    10921 tgatagatgt tgcaaagcaa aagctgatga acgacccgac gttcaaacat ttatccgagg
    10981 actgtcaagc ctactatttt gactttgaag cctatgcttc tcacttgcag gaacacggga
    11041 aatttttagt aacagaacac ggtatctttg aactgccaga gtaggaaatg aggtggtgct
    11101 tatgattgat gatatggcgg tttacattgc taatcttggc aaatacaatg agggctattt
    11161 agtcggtgct tggttcacat ttcctattgg cgaggaagat gtaaaagaaa aaatcggctt
    11221 gaatgaacag tatgaggaat acgctatcca tgataccgac aacttcccta ttgcgattgg
    11281 cgagtatgtt tccattgaag aactgaactc tatgtatgaa atgatagagg aacttcccga
    11341 ctatatcgta gaatgtctgg acgaatttat cagccactac gggacgctgg aagaagtcgt
    11401 ggaacacaag gacgatattt attattatcc cgactgtgaa accatgacag acgttgccta
    11461 ttactacata gacgaattgc aggcacttgg ggatattcca cccagcttac agaattacat
    11521 tgactatgaa gcctacgggc gagatttgga tatgggcggt tgcttcattg aaacaagccg
    11581 aggtatgtgc gagataccat attaacgctg gaagatcagc aacaagcatt gttgctactg
    11641 acagcgtatt tctcttttaa gggacagtct gaaacatggc tgtccttttc tatttagaaa
    11701 cttacgaaag gagctgaaag aacttgaaaa agattaaaag ctacacgggt atctggaacg
    11761 tggaaaaagt cttatatgca atcaatgatt ttaacctgcc ttttcccgtt acttttacac
    11821 agattacatg gtttgtgatt acggaattta tcatcattct gtttggggat attcccccac
    11881 tttccatgat tgagggagca tttctcaaat acttcggtat tcccgttgct ctcacttggt
    11941 ttatgtcgca gaaaaccttt gacggaaaga agccgtacag ctttttgaaa tcacagataa
    12001 cctatgccct gcgaccaaaa atcacttatg caggaaaagc cgtaaaactg cataagcaga
    12061 tactcaatga aacaatcacg gcagtaagga gtgtgaacta tgttcccgat aaaatatatt
    12121 gacaataacc ttgtctggaa caaggacaat gaggtgttcg cctactatga gctgataccg
    12181 tacaactatt ctttcttatc cgcagagcag aaatttatcg tgcatgacag ctttcgacag
    12241 ctcattgcac agtcccgtga gggtaaaatt catgcgttgc agattgccac agaaagctca
    12301 atacgaagta tgcaggagca gtcaaagaaa ctggtaacgg gaaacttaaa ggaagttgcc
    12361 taccagaaga tagacgaaca gaccgaagcg ttagtatcta tgattgggga taatcaagtg
    12421 gactaccgct tttttcttgg ctttaagctc atggttacgg aagaacagct caatctgaag
    12481 aacatcaaaa aatcggcgtg gctgacgttc acggaatttc tccatgaagt gaaccacacg
    12541 ctgatgaatg attttgtttc catgccgaat gatgaaatca accgttacat gaaaatggaa
    12601 aagttactgg aaaataaaat ctcccgtcgc tttaaggtgc gtcgcttgga aatcaatgac
    12661 tttggctatc tcatggaaca tctttacggc agggacggta tcgcttatga agattatgag
    12721 taccagctac caaagaagaa attgcagaaa gaaacgctga taaaatacta cgaccttatc
    12781 cgtccgacaa ggtgtgtgat tgaggaaagc cagcggtatt taagattgga acatgaggac
    12841 aaggaaagtt atgtgtccta ttttaccgtc aatgcgattg taggcgagct tgattttccg
    12901 tcaagtgaaa tcttctattt ccagcaacag caattcacat tccccgttga tacttctatg
    12961 aatgtagaaa tcgtggaaaa tcggaaagca ttaacgacag tccgcaacaa gaaaaaggaa
    13021 ttgaaagatt tggataatca cgcctatcaa gctggaagtg aaaccagctc aaatgtggtg
    13081 gacgctttag acagcgtgga cgagctggaa acggacttag accagagcaa agaaagtatg
    13141 tataagttaa gctacgtcat acgggtgtct gcacccgatc ttgacgagct gaaacgccgt
    13201 tgtgatgaag tcaaggactt ttacgacgac ctcaatgtaa agctggtgcg tcctgctggg
    13261 gatatgctgg ggcttcattc tgaattttta ccagccagca agcgatatat caatgattat
    13321 gtgcagtatg taaaatcaga ttttctagca gggcttggtt ttggtgcgac acagcagtta
    13381 ggagaaacta cgggtatcta tatgggctat tctgttgata caggaagaaa tgtgtacctg
    13441 caaccgtccc ttgccagtca aggtgtaaaa ggtacagtta ccaacgcttt agcgtctgct
    13501 tttgtcggtt cgcttggcgg tgggaaatcg ttctgtaaca atcttctggt atattacgcg
    13561 gtgctgtttg gcggtcaagc acttctgtta gaccctaaaa gtgagcgtgg caactggaaa
    13621 gaaacacttc cagaaatcgc ccatgaaatc aatatcgtaa acctaacaag cgataaaggc
    13681 aatgcaggac ttcttgaccc gtttgtgatt atgaagaatg taaaagacgc tgaaagtctg
    13741 gcaatcgaca tcttaacatt ccttacgggt atttcctcta gggacggcga aaaattcccc
    13801 gtacttcgta aggcggtacg ctccgttacc cagagcgaca gtcggggctt gctccatgtg
    13861 atagacgagc tacgccgtga agatacaccc atatcaagaa atatcgcaga ccatatcgac
    13921 agcttcacgg actacgactt tgcacacctg ctgttttcag acggtacggt agaaaatgca
    13981 atcagtctgg ataaccaact caatatcatt caagtagcgg acttagtatt gccagataag
    14041 gacacgacct ttgaggaata cacgaccatt gaattattgt cggtgtctat gctgattgtg
    14101 attagtacct ttgcccttga ttttatccat tcggacagaa gcatttttaa gattgtcgat
    14161 ttggacgaag cgtgggcgtt cttaaacgtg gcacaaggcg aaactttatc aaataagctg
    14221 gttcgtgctg gacgtgctat gcaggcaggc gtttattttg ttacacaatc ttctggggac
    14281 gtgtcaaaag aaagtctgaa aaacaatatc ggcttgaagt ttgcctttcg tagtaccgac
    14341 ttgggcgaga taaagcagac cttagaattt ttcggtatcg acaaggacga cgagaacaac
    14401 cagaaacggc ttcgtgattt ggagaacgga caatgtttat tgcaggactt atacgggcgt
    14461 gtcggtgtgg tgcagataca cccagtcttt gaagaactgc tacacgcctt tgataccaga
    14521 ccgcccgtgc agagaaatga ggtggagtga tgaaagaaag gataaaaggt gcgttcacaa
    14581 aaaagaagat tttccacttt ctcaaaatgt ctctgtttgt cgtggcactc tcccttatcc
    14641 tgctttcact tctggggacg gtggctcatg cgacggggct tgtggacgat accataaacg
    14701 cagaaaatct atactcaaaa tatccccttt ccaactacca gcttgatttt tatgtggata
    14761 atagctggtc gtggctaccg tggaactggc tggacggtat tggaaaatcg gtgcagtacg
    14821 ggctttactg cattaccaat tttgtctgga cgataagcct ttatttaagc aatgcaacgg
    14881 gctatgtggt acaggaagcc tataagctgg acttcatcaa tgatatggca gacagtatcg
    14941 gcaaaagcat acagaccctt gcaggcgtaa cgcagaacgg cttttcttct acgggctttt
    15001 atgtcggttt cctgcttctc attatcttag tggtgggaat gtatgttgct tatacgggac
    15061 tgataaaaag agaaaccagc aaggcacttc acgctgttat caactttgtg gtggtgttca
    15121 ttctgtccgc ttcttttatt gcctacgctc ccgattacat caagaagata aatgaatttt
    15181 catcagacat cagtaccgct tcacttgatt tgggaacaaa aatcatgctc cccaactctg
    15241 acagtgaggg taaagacagc gtggacttga tacgggacag cttattttct attcaagtag
    15301 aacagccgtg gctactctta caattcggga acagcaacgc agaagaaatc ggtacagacc
    15361 gtgtggaagc tctggtatcg gcaagtccag aggacgagga cgggaaaacc agagaggaag
    15421 tcgtgaaaac agagattgag gacaacgaca acaacaatct gacaataccg caggtagtga
    15481 accgtttggg tatggtgttt ttcctcttgt tcttcaactt aggtatcacg atatttgtat
    15541 tcttgcttac gggtatgatg ttgttcagcc aaatactttt tattatcttt gcaatgtttt
    15601 tgcctatcag ctttctactt tccatgatac cgagctatga aagcatggca aagcaggcaa
    15661 tcgtgagggt atttaatacc ataatgacac gggcaggaat aacgctcatt gtaacggtgg
    15721 catttagtat ttccagtatg ttttacaaca tttccacaga ctacccattc tttatggtgg
    15781 cgttcttgca gatagtatgt ttcgctggta tctacatgaa gctgggcgat ttaatgagta
    15841 tgttctcttt gaacgctaac gacagtcaaa gcatgggacg caggatattc cgcagaccgt
    15901 atctgtttat gcgacatagg gctaggtgta tggaaagacg gcttgctggt gcggttactg
    15961 ctggcggtgt tgctggtgcg gtggcaggta gaacgatttc aaacagaaaa ccgacaagga
    16021 acacagctac aaaagacagt cggggcaata ccacttccag catgggacag cgtgcaggtt
    16081 caaaggtggg ggctgtctta gatacgaaaa ataaagtgaa agacaaggca aatgctgtca
    16141 aagagaatat caaggatatg ccgacacaga ccgtttatgc ggtgtattcc gcaaaggaaa
    16201 aggcaaagtc cagcgtgtcc gacttcaagc gtggcatggt gcaggaacag cagtccagac
    16261 agacgggacg cttggaaaag caggaacagc atagacaaaa tatcgctgac aagcgtatgg
    16321 agcttcaaaa ggcacaagaa gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa
    16381 cgggagctac ccgtccccat gagcgaccag ccacagcttc aaagccgagt gcggaaaaaa
    16441 tgcaggaagt caaacgtcct gccacagcga ccacttcaaa agcaagtgag ccagtcaaga
    16501 caaatgttat caaagagcgt ccgttatttt ctggtgcttc tgataagaaa gcgacccagt
    16561 cggcacagcc agtacatagg cagaatgtag aaaaagtggt atcgcaggaa acacgccaga
    16621 attacacaaa agaccgcagg acaaaggttc agcagacgca aaccgtccaa aagaaccagc
    16681 agacaacaga gaaaactcct aaccttgtga tgaagaaagg acagaagaaa aaatgaaact
    16741 gaaacatatc gctatcattg gcagtctgtt tcctatcctc ttttctctgg tgcttttctt
    16801 tggtgtgttg attagtgcgg acagcgacga cgagaacagc aatttttctt ctggcattac
    16861 gggtatgaac ttatccgcag aagtcttgaa acatcagcct atggtagaaa aatacgccag
    16921 agaaaacggt atttccgagt atgtcaatgt gctgttggct atcattcaag tagaaagtgg
    16981 cggtacggca gaagatgtta tgcagagttc ggaaagtctt ggtttaccgc ccaactcttt
    17041 agatacggaa agttcaatca agcaggggtg taaatatttt gcgtccctgc tttcttcttg
    17101 caaaaatcaa ggtatcgacg atttgaatgt agcgatacag tcttataact atggcggtgg
    17161 ttatgtggga tatgtggcag gaaaaggaaa gaaacacgct tttaatcttg cggaaaattt
    17221 tgcccgtgag aaatcgggtg gaaagaaagt aatctacacc aacccgatag ctgttgcgaa
    17281 gaacgggggc tggcggtatg gctatggaaa tatgttctat gttgaattag tcaatcagta
    17341 tttaactgtt tcgcaggtat cgggagagct ggcacaaaag gtaatgaatg aagcgttgaa
    17401 atatcaaggc tggaaatatg tgtatggcgg tagcaatccc aacacttctt ttgactgttc
    17461 gggactgacg caatggtgtt atggaaaagc tggtatctcc ttaccgagaa cggcacaagc
    17521 acagtatgac gctacccaac atcttccact ttcgcaggca aaggctgggg acttggtgtt
    17581 ttttcattct acctataatg ctggaagtta tgtaacacac gttggtattc tcgtttcgcc
    17641 gacacagatg taccatgcag gcaacccgat aggatatgca gacctaagta gtagttactg
    17701 gcaacagcac ttaatcggtg caggacgagt aaaacaatag aaaggacttg aaaatatgtt
    17761 taagaagaat aagaaacaga cagaaactat caaagaacca aaagaaaaaa aggtgcgtac
    17821 tgttaaggta ggcacacata agaaagccgt gattgcgttg tgggtggtac ttattgcaag
    17881 cgtgagcttt ggggtgtata agaattttac tgctatcgac cagcacacga cccacgaaaa
    17941 agaaatcatt gaacttcgct tgcaggacac caacgggata gaaaatttcg tgaagaattt
    18001 tgcgaagtcc tattacacat ggaataacag taaagaagcg attgaatcaa ggacgcaggc
    18061 aatcaacggt tatctgacaa aggaattgca ggacttgaat gttgatacca ttagaacgga
    18121 tataccgacc agctccacag ttacagatgt gattgtatgg agtatcgaac aatcgggaac
    18181 ggacactttt tctgctacct acgaagtaga tcagcagata aaagagggag aaaagacaag
    18241 caatgtgaaa gcaacctata ccgtaaaagt ccatatagac gctgacgggg atatggtaat
    18301 cgttcagaac cctacccttg caccagcaat cgaaaaatca gactatgagc ctaagacacc
    18361 agaagcagac gcaagtgtag acgctgatac tgtaaacgac gctaccgcat ttctggaaac
    18421 attctttaag ctgtacccaa cagccacaga aaaagaactt gcctattatg tatctggtaa
    18481 tgtacttgaa cctatcggca gggactatct gtattctgaa ttggtaaacc ctatctttac
    18541 aaaggacggg gacaatgtga aagtcaaggt agcggtaaaa ttccttgata atcaggcaaa
    18601 agcaacacaa gtatctcagt atgagcttgt gttacaaaag gatagtaact ggaagattgt
    18661 aggataaaga aatatggcgt gcatttctat aagagatttg cacgctatcc atgttgtaag
    18721 gaattgacaa ttcttatttt ttgcattata ctattcatgt aaatatgaaa agtatgattt
    18781 acaaataaat atgataataa ggagataaat gatgaaaaaa ggacaacata tttttggaac
    18841 ggtaaaagtt ggagaacggg gacaaatagt tatcccaaaa gaagcccgtg aaatttttga
    18901 tattaacgct ggcgatacac ttttagttct tggcgacgaa gaacaaggaa tagcgattgt
    18961 caaggcagat gtaatgaaag aactggcagt aaaaatttta aaagggcttg gtaatttgtg
    19021 tcttgacaac tgaaccacta tattgagtgg tgggtttaat gatgaaaaat agcatttctc
    19081 tattcttgcc attagcctta ttgctggatt taactatccc ttttttcctc gctccgtttt
    19141 ataaaggtta tagtcattct atgcaggtaa tgagcgtgct tggaaataac aaatctccac
    19201 tacattttat atacaactta tggctagttt tatgcggtat cattctttta atgagtggtt
    19261 ttaagattta tgaaattgtt tcgcagaaat caaaaaacat cgctgttatt ttgtttatag
    19321 tcatattgat atatgcgata ggtggctgta ttctctcagg gattttttcg gtaggagaaa
    19381 caaaatctct caatactatt cctgaaaaaa tacatggtta cggggcggtt attggattta
    19441 tgtgtttgac ttttgctccg cttttgatta gcttatattg gtttcgaatt aaggaaatac
    19501 cacttgcact attttcaatg atttgttttc ttctagcatt tatctttttt acgatttttg
    19561 tgatggcaga taaaccaaac tacaaagata caatcatagc ttttgagggc ttatggcaac
    19621 gattatcact actgtttatg tatttacctt tgggttgttt aatattattg gaaaaataaa
    19681 ttaggagtga actctatgat aggactaaaa aaacgagata taaaaaaagc cttgaaagct
    19741 ggtgcaaaag ctggcggtgt atcattggct gatgttcggg ctgatattga agcgacgatt
    19801 gatgaagcta tgaatagtac agacccagag gtgcaagcaa attttaaaaa gtattttggg
    19861 aataaacgcc ctgcaccaga agaatatatt tatataatta caaagaaaac agttaaaggg
    19921 ctgaaagtat gaaaatagaa cgtctgatag gaataatctt ttatttaata aatagagagt
    19981 gtgtaacaac aaatgaatta gcacaaaagt ttgaagtatc tcgcagaacg attttacgag
    20041 atattgatac actaactctt gctggtatac cgatttattc tgagttaggt gtaaatggtg
    20101 gctatataat aaataaagat tttaaggttg atgaaaaaat tattgacagc aacaataaag
    20161 aatatatttt gcttgcttta aatagtctaa gaactgttta cggaaataaa aagatcattg
    20221 aaacttacga aaaaatgaaa catctttata acgatgtcaa tattaatgca cttccagatg
    20281 ttgatttctc tgttgtaact gaaagtccac aaattataag gtgcgtagac ttaatcaata
    20341 aagcaacaaa agaacatcga actataaaat ttatatatac taattcagca ggacatacaa
    20401 aaaaagtttt tctaaaccca ttacatacat attataggtg gtactcctgg tatgtttttg
    20461 gatatgacac ctctaaaaat gattatcgca tgttcaaagt ggtaagaatg aaaaatattt
    20521 ctttaacaaa agaacttttt caaaccaaag ataatatagc tgacctttta tctgattttg
    20581 aaaaaagacg caaccagaca aatattacgg ttactctatc ttataaacaa gaaatagaca
    20641 ccttagttaa tgaatacttt aaaggaaaaa ttgttgaaac tgtcaatgac tgttttataa
    20701 gagaaattga gattaaagaa aatgatttta tgacattctc aatcctttta ggactttgcg
    20761 ataaagtaaa aatattatcc cctcaaaaat acaaagaaaa ggtattaact catatcagaa
    20821 aaataaagaa aaatttcact taatagtgac atacatttgt cactattcct gccatataat
    20881 tttcttatat cgaaaatatg aggagatgtg aaaatgaaaa aagtatatgt atatttatta
    20941 gacgacatgg cagaatgcga aattggttat attttacaag cattcagtat ggaaaaatta
    21001 ttaaaaaatg gaactaaaga atttgaagta aagacagttg gttttaataa agaacccgtc
    21061 ttaactttag gaggattaac aattgttcca gattatagta tatcagaagt tgacttcaat
    21121 gctagtacag ctctgctttt accgggttct tccacttggg gaagtaaaga aaatcaagaa
    21181 atattggaaa aagcacaaga atgtttaaac aaaaatattt tagtaggtgc catttgtggg
    21241 gcaacactcg cacttgctga ttatggtata ttagataaat ttaaacacac aagtaattct
    21301 ctagaatatc taaatttctt ttcaagaaac tatgcaggac agagcttgta cgtttgttct
    21361 aattccgttt tagacagaaa tttagtaaca gcaaattcag caggttcatt agaatggacg
    21421 aaaaatattc taaattacct taatgtgtat tctgacaata aaattactgc tttttacaat
    21481 tattactcga caggaaacgc aaaatactat gatgaacttt taaaataaag gtggtgtttc
    21541 tgtaatgaaa caatcatggg tattatgccc atctgcaata acaagacacg aacaaaaata
    21601 cgggtagata cggaactgat aagtttccct ttgttctgcc caaagtgcaa gcatgaaact
    21661 ttaatcaata taaagcatgg aaatgtaacg actattaaag agccagacgc taagacgcag
    21721 agccgatagt ttataggtta ttgtaactta tagattgttg gctcttattt gttacctgcg
    21781 gagcggatca gcaaataagt tttggaatat aatggaaaaa catttatata aaagaagaaa
    21841 catgaaaata ttagtaagtg cctgcctttt acaatataaa tatactaaaa aaatcgtaaa
    21901 aagatcctca ataattcata tatttttact aatgaaagat caatatgaca taccaaagcg
    21961 ttatttgctt ctattcaaaa gagtgtcaat ttcttcttct gataattttt gcttgaaatt
    22021 aaacacatta tctacagctg ttcctaaaag tcttgccaaa gggcttagta atgaaatatc
    22081 aggatatgta ttgtttgttt cccattttga caccgaagaa gtagttattc caagagcatt
    22141 tgcaacttgt tcttgtgtta gaccttttat ttttcttagt ttctgaataa tatcaccgat
    22201 aaataatttc attttgtgac atctccttta cttaatgaaa atattatcag ataaaaactt
    22261 tttttaacaa tgcaggtatc gcagtatgaa cttgtgttac ataaggatag taactggaag
    22321 attgtaggat aaatcaaaaa atgccgtagc tttattttgc atttattgca agttacacgc
    22381 tacggctaga ttaaaatata atattttgaa atcttcataa aatctttaat tttgcttttt
    22441 atactttaac taaaaaataa ggaaagggta tgaacaatta tgaaagcaat tttaaaaaca
    22501 aataacctat gtaaagattt caaaaagcaa aaggcagtga aaaatgtatc tataactgtc
    22561 cgtgaaaatt caatatatgg gctactcggt ccaaatggtg ctggaaaatc taccactttg
    22621 aaaatgatta cgggtatgct ccgtcctact agcggtaaag ttctttttaa tggacatgaa
    22681 tggaacagaa aagatttaga acagatagga gctttgattg aaactcctcc actttatgaa
    22741 aatctttccg cagtagaaaa tttagaagtc cgtgcaaaat tactaaatat tcctaaaacc
    22801 cgaatagata aagttttaaa aatggtagat ttgcaaaata caggaaggaa aaaagctgga
    22861 caattttcaa tgggaatgaa acaacgtttg ggaatagcta tcgctttgtt gaatagtccc
    22921 aaattgttga ttttagatga gccgacaaat ggattagacc ctatcggcat acaagaatta
    22981 cgttctctta ttcgttcttt tccctctaaa gggattactg ttattctatc cagtcatatt
    23041 ctatcagagg ttcagcttat cgctgatgat attggtatca tttcaaatgg tattttagga
    23101 tatgaaggac agatgaataa agatgaaaat ttagaaaatc tgtttgtaga agttgtaagg
    23161 aaatcacagg aggaacgata aaatgaaaaa ttatatatct tctgaatatt taaaacataa
    23221 aaatacattc gcaaaaaaat tagtatggtt agctcctata ctaactttat gtctgaatat
    23281 acttgctcct atgtggtatc aacaaaattc gtataactgg tggtatgtat tcttatatcc
    23341 tggttgttta acgctattat ctattttgat aattcaaaga gataacggca aattaaaatt
    23401 tagagcaatt ttgtcattgc ctgttgactt aaaaaaagta tggtatgcaa aaataatcac
    23461 ttgtataata tacattgtac ttgcaaatac aattttgatg ttatgcaatg ttgcaggtgg
    23521 ttttatcatt caacacattt ttgatatacc tatgacaatt agcccaatgc aggcattgtt
    23581 cggaacactt tgcattattc tggcaagtat atggaatatt ccattatgct tgtggtttac
    23641 aaaaaaagtg ggtgtatttg ccactctcat attaaatgtc ggaatgagct ttatgttggg
    23701 aacattaggg gcaaatacaa gtttttggtt catatgtcct tatagctggg ttactcgcct
    23761 tatggttccc acattaggaa tattaccgaa tggagaatca gtaacaggaa gtgctttatc
    23821 aactccttta ccgttggtta ttatgacaat ctgcttgtca ttgcttttac tatatacaat
    23881 ttcaaagtca acggcaaaat cttttataaa gcaggaggtt ctttaatgtt tagtattatc
    23941 agatgttttc aagcagaatg gcttaaaagc agacatacac cattactttt agtccatgtg
    24001 ctttttccgt taataggagc tggtgttttt gcaggatatt ttcatatatc cggttggagt
    24061 gagatgacaa atgtaacgac ctttttagag gtgttagcaa ttatttttcc atttgttatt
    24121 ggtatcgttg taggtatgat agtcgaacta gaaaatggag caggtcattt tcagttaatc
    24181 ttaggtacga tccgttcacg tatagcggta tatatcggaa aattagtata tcttatcgtt
    24241 ttagcaacct ttgcaacaat actttgtatt ttcttgtttg ccgtactcta tccagttatg
    24301 ccattttcgt tttatataaa acctcttatt atgttgatat tgtctgtaat accactttat
    24361 cttatttcca tacctgttgg tttcagttta ggcaaaagtg tatctatggg cataggtatt
    24421 gtaggaagtt tattatctgc attgctagtt acaggacttg gagattttat ctggaaattc
    24481 ctgccgtggg gctggggtgt acgatttatg gattattgtg ttttaagata tgttaatgag
    24541 gggcagtttc attctaactt acccgaattt caactaggtt ttatactcat gctggttttt
    24601 acagtagtgc tgtttatcat aagcttagtg tggtttcaga gatgggaagg agtaaaagaa
    24661 aatgagtaaa aaaatgatga tattgctgtt tgctgttatt atatgtttat ctcttgcagg
    24721 ctgttcttta caggacaaaa tcggggacta ttccagagag caatgctatc tgaataagga
    24781 aaatgtgacg caattttcct ataagggaaa tgactacacg attttagcgg atactgtctc
    24841 taatagtgga ctcggggaat ggattgggta tatccgacaa cttgcggctg tagacgaatc
    24901 aggaaaaata ttacttcaag aaaatctcaa aactgctacc tttcagacgt tggcagattt
    24961 agcagattta gtggataaag cccctaatga cgcctatatt attccattct taaatgtgta
    25021 tgcagctcct aacgctgacg actatctgat tgtagatata aatggagggt atcacaaagc
    25081 tgttatagat gaaaatatca aaggcacaga tacagttttt gactttaagg atatagagca
    25141 atctatgagt ggaaagtttg aaatcaatcc tcaaaatgca acgcagcttc tttgtgatgg
    25201 gacggtttat caagtaactt ctgatactgt atcaaataat gaattgggaa gttacattgg
    25261 tattcttgcg gagaatgtca tatttaatgc agaaacaaaa attcctttgt caaaggagga
    25321 actaagaaaa attgactggt atggggaaaa cgccggacaa catcgggaac aatggattta
    25381 caaagatatt tatgaaattc atggtacaga aaagaccgaa gccgttgcag tacagataaa
    25441 tgaccgctac tatattgcaa agcgtcaatg accgattttt gtttttctgc ctttatggat
    25501 tatgatatac taaaaaagct aggaggcgat actatggcaa caatccttat gattgatgat
    25561 gaacgaccta ttttagaact tgtgaaaaat ggactgcaaa aggacggaca tttagttaca
    25621 gtctatacgt ccgccgcaca ggttcctctt gataaactga acagatatga cttgattata
    25681 ctggacatta tgatgccgga tatagacggg ttttcttatt gtgataaaat ccgttcatta
    25741 gtggactgtc ctatcctctt tctgactgct aagacaatgg aaaatgatat tacattcggt
    25801 cttggattag gagcagatga ctatttgaca aaaccattcc gtattacgga attacgggca
    25861 agagtaaatg cccacttacg ccgcgaacac cgagaacgac atatagctct tacatttgac
    25921 cggataaaaa ttgatttatc tgcaaaagaa ttacgggtag ataatatacc agtgccttta
    25981 acgaaaagtg aatatctgat ttgtgagtat cttgctagta acaaaggaca ggttttttca
    26041 aaggaacaaa tatacgaagc tacattttcc ctttatggag aaagcgatag ttctacaatc
    26101 tcgacacata taaaaaatat aaggtcaaag tttgagaaaa cgggtgttca gccaatcgta
    26161 acagtttggg ggataggtta tagatgggaa taaaaatgaa aacatcactt acaagaactt
    26221 tttataaatt tcttgtttgt ctacttggcg gactatgctt gtccgttgtc gtcccttttg
    26281 cctgtatgat gtttgcttcc agtatagggc ttgtaacact tgcagataat aacgaacggc
    26341 aaacgaaagc tttagtaccg atactaactg caacaccaaa tgtatcagat atacattttc
    26401 ctactggaac taaatttctt cgattaacta aaaactatgg atacatagat acgaatatga
    26461 gtgaagaaga aaaagaaaag gctttaatgt ttgctaaaac ggggttcatg gatacaagtg
    26521 gtaaaacaca gtttattttc attactcgag atgatgaata tattgtttta cagtatgtta
    26581 taggttctca atatttaaat gcacaattta atcagtattt accgtcccca gaaattatga
    26641 tgatttcttt aatcactata agcagcttag gggtatgtgt ttatctgact gttcattttg
    26701 ctaaagagct gagaaaagaa ttgacaccta ttttagaagc taccaaagaa attgagaata
    26761 acaacttaga ttttactatc ggacattcta aaatattgga atttgaaaac gtgattatat
    26821 cctttgatag tatgcgaaat agtttaaaag ggtcattaga aaaacagtgg cgaagtgaaa
    26881 aagcacaaag agaacagatt gcatctttag ctcatgattt gaaaactcca ttaactgtta
    26941 tgcaggggaa cattgaccta ttagatgaaa cagaacttga cgaagaacaa aagctatatt
    27001 tatcgtatgc aatgagtagt tctgagcaga tgaaacagta tataaaaatt ttgattgata
    27061 tatctaaagc atctgctggc tatcaattac aaatggaaga tgtaaatttt ccagagtttt
    27121 ggaaacatgt cttatctcaa actgaaatca tctgcaaaga taaaggtatt gttattcagc
    27181 agacacagca taatttccca cagaatataa ctggggatag aatgttactg gaacgagcat
    27241 tgatgaattt ggtttcaaat tctttagagt actcgcctga aaactctaca ttatatatag
    27301 atgtagatag caaggacaat tacttgaata tctgtattac agactgtggc tgtggctttt
    27361 cgcaagaggc attggatcac gcaaaagaac gtttctatat ggcagaccaa agtaggagct
    27421 ctaaactgca ttatggaatg ggtttatata ttgttgatat cattataaaa cagcataaag
    27481 gaaaacttct gctagataat tccacagaaa ccaaaggtgc caaagttact ataaagttac
    27541 cattataaga gaacggctta tttgtcgttc tttttttcat atctttaaga aatcttcaaa
    27601 attaggttgt attctttgtt tgaaggtaat ggttatatgc tattatacgg gggattgtgt
    27661 atgaagtgtt ttaagcaatt taccaaaaac tcaagaacaa cttaacagca atccatgcgc
    27721 taatacaact gaatatttta atatcttagc tcgtacagac tatatgtttt gtgcgggctt
    27781 tttttgtacc cgaaaaaatt ttctaacttt tttcggcttc agcttagcaa atcacacctt
    27841 gctgtacaga tatggtgcgg aaagagggat aaacactttt caagtcatag ctggaaaggg
    27901 ggtgagatta tgaagctgtc tgatttccag aaaacaatac aatgtcaatt tgaatgtaag
    27961 ttaaagaaag ttacaaaagg cattgtaaga aattatcaga aagaattaaa acggagaaaa
    28021 aataaggaaa ttcctttttg tgaacttcca gaactagtga ttgaaaaatt atccgtttta
    28081 gacgattacg acagcgacta tacattattc aatgtatgtg gcaacgatat tagtattctt
    28141 gatgatgaat tggcagaagc cttgaaaaag ctgtctgacc gtaaccgtga gaacttactc
    28201 atgtactact tcttagaaat gagcgatacc gagattgcta aattgcagaa tatttccaga
    28261 agtggagtat ttcaaaatcg gtataactca ttggaactta tgaaaaaaat actaaaagga
    28321 gaaaagtaag aaatgaagca catttttaag aaaccgtcat tttatctgat ttcgtcagct
    28381 acggacggag acgaaaaagc gattgaaaag atactggcaa tttatgaccc gtacatatca
    28441 aaatgatatt gtcaatattg gtttacactt tttctcaaga atgttcgacc ttatgctgcc
    28501 tcctgtagcg aagcatagta ttgttgtcgc ttaaccattg gaggtagtcc accattagcg
    28561 gagcagatcc tccgattatt ccagtagctg atgaagtatc tccagatgag cgtctttaac
    28621 tgctctacgg tcattgaggt gggatcgtaa cgaccataaa gtaattcctc tttgaagcgt
    28681 gcccacatgc tttcgcatct ggcattatca tggcatctgc caccggcact gttcatgctt
    28741 tgaacaatgc catatctgtt gatagcttta cgatatagct cactggtata ctgggttccc
    28801 ctgtcactgt gaagaatagc tccacggagc atcgggtaag ctttgcaagc attctctaag
    28861 gtttgttcgc atagggtagc tctcatattt gtatccatag caagtcccaa tactgctaaa
    28921 tcatagcagt caaagatagc tgaaacatag agcttcccgt cagaagcctt tatttcagtc
    28981 atatcggtca tgcacttttc aagaggcttt tcagcagcga agtcacgctt gattaaatcg
    29041 tcggatttac tggcttctct atcagctttg gtaataccat tcggcttacg ttttggttta
    29101 tggttaagac caatttctgc catgacacga taaacagttc tctcaccggg aatatgtact
    29161 ccctcaggct gtttgagctg taaggcctga tacatacgga ttcttccata agtgtcattg
    29221 cattcatctt cgctgcaaat atcaagcatt gcatctgcca gtgcctgata tttccaaggg
    29281 atatcttttg tttttaggaa tttgttgaat gcctgccttg aaacatgaag cactttgcaa
    29341 taaaaagaaa ttttaccctt aatccggccg tcatccgttt tgattgcaat aaacattaat
    29401 ctctgttttt tgctgacttc cgacggctgg ctgcgaaaaa agcacttgct tcctcaagga
    29461 attcgttttc ttcttttaag cgacgaattt ccttatcctg ctcttttaca cgctttctga
    29521 gttcaataag ctcatcgtta agggataaag cattcttggg cgtatgaact gcttcatttg
    29581 cactgagacg cccctctttg aaggctttta tccaagtgta catagtaccc tttggaatcc
    29641 ctaattcctg agcagcttta tgaccgccga tttcctgggc aagctttacc gcctgtgctt
    29701 taaattcatt gtcataagat ttttgattct gtgccatagg tttttctcct gttctcgtat
    29761 tgattatgat tatatatcaa aatccttgag aatagggtgt caacttttat gatacaacac
    29821 caaaatgttg tttgcgttca ctctatgatg aatacggtaa tgtttatatt gccgtggata
    29881 tggaattaaa aggacgtatc agagaagccc ttatcaaaat gattttggac tttgatatac
    29941 ctttagaaac cgaggaagaa taacaagcag taaccgcaga gcatgatttg ttcaatcccc
    30001 tctttcctgc tctgctccct gcttttacat gaaagcaaca tgctttcgcc acagctcttt
    30061 gacaactgaa taaagcagac agatacgttt gtgagatagc gagccacggg gactgtacgc
    30121 cacgaccttt tcaaaagaaa gcgagcgacc cacgcagtga tccgagagcg actgttggaa
    30181 aagtcgtttt gccacgacct atcctcacag aataatgata cgtccgcatg gtgcggttct
    30241 gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg tctgcctgct
    30301 tgtaaaaaat gacacacagt aaagggggtg catagattat gaacaatact gatgtgccta
    30361 tctgggaaaa atatacgctg acgattgaag aagcgtctaa atacttccgc attggcgaaa
    30421 agaaattgcg taaattagcc gaagaaaata ttgacgctgg ctgggttatc gtgaacggta
    30481 atcgtattca gattaagcga aaacaatttg aaaaaatcat agatacattg gacgaaatct
    30541 aatgtcattg agccgtagat atggtataat aaggtatagc gtatcacggc tcattccatg
    30601 acggaaagga gctttacact atgtctaatg taaaacgaaa agacagtaaa aatcgcaatt
    30661 tgcgtaatgg agagagccag cgaaaagacg gaagatacgt ttataaatat accgatatat
    30721 acggaaagcc acagtttatc tattcttgga aacttgtacc gacagacaag acacctgctg
    30781 gaaagcgtga tgatatatcg ttgagggaaa aagaagcaca gataaaaaaa gaccttaacg
    30841 acggtatcga cacagtcggc ggtaaaatga cagtctgcca gctctacgag aaaaagaaca
    30901 gtcaaagaaa gaacatcaag agggctactg aaaaaggacg acagtatctt atgaacgctc
    30961 taaaaaatga cctgttgggt atgagagcga ttgataccgt caaacagtca gacgctaaag
    31021 aatgggctat cagaatgagt gaaaaaggat atgcctataa aacgattgat aactataagc
    31081 gttccttgaa agcgtcattt tatatggcga tacaagacga ctgtatcaga aagaacccgt
    31141 ttgaatttaa gctaagtgat gttctggaag atgatacgga gcagaaagtt atccttacac
    31201 cagagcagga aaaacacctg cttgccttta tggaaacgga caagatttac agtaagtatt
    31261 atgatgaggt tgtgcttctg ctggaaacgg gacttcgtat ttctgaattt tgcggattga
    31321 cgacgcatat tgatatgcag aacagaatac tcaatataga ccaccagtta ttgaaagaca
    31381 gcgaaatcgg ctactatatt gaaacgccaa agaccaaaaa cggaaaacgg gaacttccat
    31441 tgacagaacg ggcttatcaa gcaatccaaa gaatactaaa gaacagagga aaggcacaac
    31501 cgctgattgt aggtggttac agcaatttcc tattcttaaa ccgtgagggc ttgcctaaag
    31561 ttgcaggaaa ctatgagggc atggtgcgag gactaattaa gaagtataac aagtatcaca
    31621 cggataagtt accgaacatc acaccacatt cattcagaca tacttattgt acgaatatgg
    31681 caaatagagg aatgaaccca aacaccctgc aatatctcat gggacacgct aacataacta
    31741 tgacactggg ttattacgca cacggtacat ttcaatctgc aaaagcagaa ctggaaagac
    31801 tggcttgtta atatcggagc ttatatttac tactcattta ctacttttgg ttgcattttc
    31861 atgctggtaa atgccagctt atgcaaggta tcttccgaaa agaaaatacc gataaagccc
    31921 taaaatacgg gatatatcgg catttaccaa cttatgaaaa gataatcaga aatctagtaa
    31981 gtttttataa taaaaatact atattttttg ctcttggaat ctcaacaaca ttctttatat
    32041 tgggttcatc agtaaaagta ttgagtatgt tttttaatga aaataaagat ttaataatgt
    32101 ttattggtgg gattataatt attatcatgg gattatttta catgggaata attaaatctt
    32161 caatattgaa tagagaaaaa agatttaatg ttaagtttaa agaaatgaaa gcaataacag
    32221 cttttatatt aggttttaca tttagttttg gatggacacc ttgtattggt ccaatattgg
    32281 catctgtatt agtcatggtt tcaagttcaa gtaatcatct tagtgcgaat cttttaatag
    32341 cagtatatac tattggtttt attttgccat tcataattac agctatgttt tacagtaaac
    32401 tatttaaaac tatagataag ataaaatcta acatggaaat tattaaaaaa ataggtggga
    32461 ttatacttat agtatcagga atattaatga tggtaaatgg atttggaagt ataagcaaac
    32521 actttaatac gtcacaaaat agtaaaattg aaagcaagca agaagaaaat aagcgagaaa
    32581 attctactga taaagaagaa aattctgatg gtaatgatag tcaaaaagac tctaataatg
    32641 acaataatga taaagggtct aatgatgaag atagaataaa gtctattgat tttactttaa
    32701 cagaccagta tggtaaaaca cataaattaa gtgattatga aggcaaagtt gtattcttaa
    32761 atttttgggc tacatggtgt cctccatgta aagaagaaat gccttatata gaacaactgt
    32821 ataaagacta taataaaaat aatgatgatg tagtaatttt aggagtagct tcacctaatt
    32881 taggtagaga aggttcaagg gaacatgtag ttaacttctt aaaagaccaa ggttatacat
    32941 tccctgttgt acttgatgaa gatggagcat tagcatatca atatggaatt aatgcttttc
    33001 caactacatt tataattgac aaggaaggat atgtaactca gtatattcca ggagctatgg
    33061 ataaggcaac tatggcatca tttattgaaa atcagagaaa taaataaaag tatttaagta
    33121 taagtttata aatcaattta aggttacaaa aaagtgccta attgtaccac ctaaataatc
    33181 ttgttatagt aaaagtataa acaatacaag aaagggttga attttatatg aaaaaaacat
    33241 ttgatgctat aataatagga tttggaaaag gtggaaaaac attagcaggg gatttggcaa
    33301 acaggggatt aaaagtagct ttaatagaaa agtcaaacaa gatgtatggt ggaacatgtg
    33361 taaatgtggc atgtatacca acaaaaagct tagaaaattc agcaaacagc gtaaaaacaa
    33421 agaatataaa ttcttgggat gaagtacaag ctgaatatga gaaagctata gataaaaaag
    33481 aaactttaat tacaaaactc agagaagcta actataataa attaaattca aatgagaatg
    33541 taactatatt cacaggaatg ggaactttca ttgatgaaaa aacagttcaa gtcaaaactg
    33601 aaaatgaaat ttatgagtta gtagcagata atatatttat taatactggt tcaagaccat
    33661 ttatacctaa tattaaggga attgaaaata agaatatagt atatgatagt gagtctctta
    33721 tgaatctaag aactttacct aaaaaaatga caattatagg agctggattt ataggtttag
    33781 aatttgctgg tatttatagt tcatttggag cagaagttac aatattaaac tctaataatg
    33841 gcattttacc aaatgaagat gttgaggatt cagaagaaat aataaaatta cttgcaaaga
    33901 gaaatgtaaa aattgttaat aatgcaaata taaaagagat taaagaagtt tctgaattag
    33961 caatagtaga atatgaagta gatggtaaat ctaaagaatt aacaagtaat atgatacttg
    34021 tagctacagg aagaaaagca aatactgagg agctagggct agaaaatgca ggtatagaat
    34081 taaatgaaag aggatttatt aaagtctcag aaactcttaa gactaataaa gagcatatat
    34141 gggctatagg agatataaat ggaggtccac aatttactta tatctcattg gatgattatc
    34201 gcatagttat taatcagttg tttggagata agactagaac aactaatgat agaaagaata
    34261 tcccaaattc tatatttata agtccagcat tttcaagagt tggtcttaat gttaaacaag
    34321 ctaaagaaaa gggatatgaa gtattagtgg caaagatgcc agtagaagca attccaagag
    34381 ctaaacagat tggcaaggca gatgggttta taaaaatagt gatagataaa aaatccaata
    34441 agatacttgg agctagtatg atatgtgaaa attcaagtga gattatccat cttattcaat
    34501 tagctgtaga tttagaagta gagtatacat atttaagaga ccgtgtttat gcacatccaa
    34561 ctatgacaga agctcttaat gatattctat ctcctaatat gataaaagaa gtataacata
    34621 agtttaatat ataaacctaa aattatcatt ataagtagat tttagaaaga ggctatctca
    34681 aaaaatagag ataagtctct tttttactgt ttaaatgata aaaatatatt tgattttata
    34741 aaagaagatt atcttatgaa ctaaaaagtt tattttaaaa cactacctat ttatgttata
    34801 aacttataaa tataatatag aatctattat tctgtattat taagtggggt taagcgttat
    34861 aattaattat aaatattgaa taatctaaat ggataagata atggtattaa aaattatctt
    34921 aaaaggtgaa ataatctgta tttaatgtag tacagttata tctttaaagc aaggtggtga
    34981 aatgatataa ataagatatc taaaagaggg tttgatgata gtgataaaag atggttttca
    35041 agtaaagaat taacaagact tataaagtct aaagaagaaa tagaatggtt attaaatagg
    35101 gattacaaaa tagacccagt cgttactttt gttggtgata gatatcagtt ttctataaga
    35161 caaagagatg cattaaaaag ggcagtctgt actgatgaaa aaaatattat aagacaatca
    35221 aaaaggcttt ctctagataa aatcaaagaa gataccgtaa atatagatgg ctttaatttg
    35281 ataatatcca tagaggtagc attatctggt ggtactctag taataggaag tgatggcaat
    35341 ataagagatt tagcaggtct taggggaacc tataagatta tagataaaac agaagaagct
    35401 ttgaatctga taggtaaatt ttttaataaa tacaatgcac agaatcttaa attttattta
    35461 gatgctccag tttctaattc aggaaattta aagtatagga tattagagca tgccaaaact
    35521 tggggaattg aaactgaagt agaattagta aaaaatgcag atgttgtatt ggaaaaatta
    35581 gatagagtag ttagtagtga tgcagtcata gttgacaagt gtataagtta ttttaatgtg
    35641 gctagaggta taattgaaga gtatattaaa gaatgtaata tcataaattt aaataaatag
    35701 ataaatatag gtgagaatat ataagtatat gtagcaatat tcttagtaat acaagtaaag
    35761 tttgaaaata ggagaacgaa tacatatcga atttcataaa atgttgtaaa aagacatatg
    35821 agaggatgga tatgtgtgaa attaactaaa ctaaatatta aaaaatatag agcacctata
    35881 tataaatatg ctttagcaaa tgtaaatttg aggtctgcta aatctactaa ttcaagtatc
    35941 ataacagtaa tacctcaggg agccaaaatg gaagtattgg atgaagaaga tgactggatt
    36001 aaggtgatgt acaattctca agaaggatat gtgtataagg atttggtatc agtaagcgaa
    36061 tatgcttgga gtaacttaaa cttaagagaa gataaatcta caacttccaa tataattaca
    36121 gtaattcctg aaaaatctag ggttgaagtc cttcaagtag atggagattg gagtaaggtt
    36181 gtatatgatg ataaaatagg atatgttttt aattattttt tatcaattga tgggaataaa
    36241 ccaaatgaat tagattataa gtatttttat actgacatga ttaaatttgt taacgaaaat
    36301 aacattaaaa gtactagcga ttatctaatt gtaacagacc ttagaaataa atatacttat
    36361 atttttaaga aagataatgg aggatgggga caactttaca agtggcaatg tactataggc
    36421 aagcctgaga ctccaactat aacaggtata ttttatataa gtggaagaaa accttctttt
    36481 ggaacggatg agtattcagt taagtatgct actagaatta aaggtggata ttactatcat
    36541 tctgtactat atgattcaac aggaagttat ataatagatg gcagacttgg tgaagctctt
    36601 agtcatggat gtataagatt aagtacggaa aatgcaaagt ggatatatga taatatacca
    36661 gatacaacaa cagttataat tcattaagaa aaattttgag ttttttattg ggataataat
    36721 tcttaaatta atagaattat atattaactt ctcataaaga gtactattta aaaagtgtaa
    36781 taagagagtg ctattaacaa aacaaagata ttttgaggca ctctcttata aatatacata
    36841 attaagatat tttcataaac aattctataa tcttatcttt atcaaagaaa acaggattag
    36901 caccagcaca agcatctttc atagagtttt ctgcaagcaa ttcaaaatta ggattatcaa
    36961 cacctacatc ttttaaagat tttgggattc caacctcttc agaaagagct tttatcttat
    37021 ctattacaaa gtcaacacac tctttatctg atttattatt tacatccatt ccaataactt
    37081 cagctattgg tcggaatttg gcaggggctg atttagcatt ttcctcctca actattggaa
    37141 gtagcatggc attacatact ccatgaggta aatcatataa acctcctaat tgatgagcca
    37201 tagcatgtac atttccaaga cctgcattac taaaggcaat tccatttaaa aaacaagcat
    37261 aacacatttg ttctctagct tctatgtcat taccattttt aacagctctt ggaaggtatt
    37321 taaatatctg ctttatagca tataaggcag ttgaatctgt aacatcataa gcaccattag
    37381 ctactacagc ttctatagca tgagttagtg catccatacc agttgctgct gttaaattag
    37441 atggtttgct aatcattaat tctgggtcat ttactgtcat tgtggctagt gaattggtat
    37501 caaccattat catctttaca tgtctgtctt catcagttat aacatagttt attgtaactt
    37561 cagcagatgt accagcagta gtagttatag ctatgatagg aagacatttt ttagaagttt
    37621 tgttgatacc ttcatagtct ctaatatctc ctccattagt ggctaatata gagatagctt
    37681 taccacagtc ttgtggagaa ccacctccta tagttataac gaagtcgcaa ttttctgatt
    37741 ttaatatttt aagtccttta ttcacattag ttacagttgg attttgttta acttcatcat
    37801 aaacaacaaa gtctacatct atctgagata aaatatctgt tacttttttt acagtaccat
    37861 ttgaaactag aaatttatca ctaacaataa atgctttttt agttcctaat gatttaattg
    37921 aatctttagc ttcattaaga catcctttcc caagtaaatt tattggtggt acataataaa
    37981 tgctcataac taaaatctcc ttttattttc attgattttt atgtaaaaat atatatctaa
    38041 caattttcac taacttactc ataatatatc gtatcttaga ttgagatagg attgttggtt
    38101 cttatataag aacttaattc gtaattacga ttatgattag aatttaacat agtattttta
    38161 tcatgtcaat aaaaatataa aaaatattat aaaaaaaaca aagttactgt agttaaatca
    38221 gcaactttgt ttttatagta taaatcaaat aaatacatta gtttaaaaca aaatttttaa
    38281 ttattatgtt ttgatatctt ccatatacag aatattaatc taatttatta caatgtcttc
    38341 attacgttct tccatcattg attgaatatt tttctgagca tttaagagta atttttttat
    38401 cagttctatt ttttcgtcat tagctctaaa taaaggcagt gttacactaa cagcagctct
    38461 tatacatcca ttttttctta ttggtacggc aagacatttt aggtgttcca ttgattcctc
    38521 acactctgaa gctatttgtg ttctttgaac ttcaagaagc tggtcgtata aaacatcaaa
    38581 atctgtaatt gatttttctg taaaaccttt tagaccatct ggatataagc ttattagttt
    38641 ttccttgtca aaatcacata aaagagcttt tccaagacct gtacaattag caggtatttt
    38701 ttttccaaca taagaaataa gtctaattgg ttctggagaa tctacttttc caatataaag
    38761 aacttcatta ttgtcaagta taccaagctg acaagtttca gaacaggaat taactatatt
    38821 agtcatttcg tcaattatat agttaaaaat agttcttttt tcaaaaaatg aagaccctaa
    38881 tgtgtaagaa cgtatcccta tagaatattt ggatgtgttt ttatcaatag taatgtattt
    38941 tctagcatta agtgtatgaa ctataggaaa gatgctactt tttggagcat tgataacatc
    39001 tgctatttct gtaagagtat atccattttc ggattctgct aatgcttcta aaatatctaa
    39061 tatcctagct gttgggcgat gattatctga agtcataaat ttccccctct ttttattaat
    39121 ttaaagtatt agaatatgtt gattttagca cactatacga tattttacaa tgtagaaaat
    39181 aaatatgtaa atttatatat aaataagctc ttgatttaga tacagataga tattttttta
    39241 gactgtacat taaggaataa ctaatttcca aaatgtacct aaagaaagta agtgatattt
    39301 agttatataa tttaagtaaa tatatgcaat agttattttt atatgtaaat atcaaaatat
    39361 tatgaactta tattttatct attttttaca ctgtataaaa actttatagt tttgaatact
    39421 atataatttt aaatacgctg tatttttgca tataaggaag tcatttgata atatacaagg
    39481 tattatcaga aagtttgaaa aaattttgac aaaaatttat tttttttatt gactcataaa
    39541 taggaaaatg ctatcattaa ttcaaagatt cgtaattaaa aacttagttc ttaaatacga
    39601 accgaaacgg ccgttagaat taaagctaaa aacttaataa gagtaattaa atatcatgtt
    39661 ttaggaggat taactttatg ataatggatt ttattaaaaa aattcctgca ggtatgatga
    39721 tagtgccaat gtttatagca gctttcatta ataccttttg cccagatatt gtacaaatag
    39781 gttcgtttac gacagcagta ttttcaagtg ttgggtctgc aactataatt ggggttcaac
    39841 tggtatgctt gggaactcag cttcaattta gagaaatgcc aaaagtttta aagagaggtg
    39901 gtatacttct tggttctaaa tttgctatag gtgctttgat aggtatactt ataggaaagc
    39961 tatttggaat ggatggtgtc cttggtctta caacattggc tgttataagt gcagttacaa
    40021 atagtaatgg tagtatatat ctttcattga tgacttcata tggagatgaa acagattgtg
    40081 caactatgag tttacttact ttaaatgatg gaccattttt tacattaata gctcttggga
    40141 cttcaggtct tgcaaatgta ccattgttgt cattattggc tgcaatagtg ccaatacttg
    40201 ttggtatgat aataggaaat tttgacaaaa aaatgaaaga gtttttagag ccagggtgta
    40261 atttactcgt accttttgta gggtttgcac ttggtagtag cataaatctt gcaagtatac
    40321 taaaaggagg tttatcagga gtattacttg gattaatatc tgtatttgta ggaggtatat
    40381 ttatagtatt ctgtgataaa tttataggaa aacgtccagg atatgcagga tgggctgtag
    40441 ctacaactgc tggaaatgca attgctgtac ctgctgctgt tgcattagtt gacccatcat
    40501 ggcaaccata tgttgcaaca gctacaacac aagtggcagc cgcagtagtt gtcactgcaa
    40561 tattagtacc tcttataact agttggtggg ctaagaagta tggatgtcct aagttcccaa
    40621 aacaaaatga ggatggtaca ataacagctt aatttatatt acagtgagtt gttggtaaaa
    40681 taataaattc tttaattaga ttttaggata ttaataaaat tatattattt ataaaggaga
    40741 gcaagaataa tgataaatgc agtgataatt gatgaaaaag ataatgttgc agtagctatt
    40801 gaagcaatag ctaaaggtag tgaaattagt tttaaattaa aagacaaaac agtaaaaacg
    40861 attacagcac tagatgatat aactatttat cataaattgg ctataaaaga tatggcccaa
    40921 ggtgataaag ttactaaata tggtgaacat ataggagaag caaatcagga gataaaaagt
    40981 ggtcaacatg tacatattca taatgttaga agtgttagag aggatttgga taaacaagtt
    41041 atgactaata tataaaataa tatttataga gttataaaag ttgtattcat tgattttagt
    41101 gtcaaatagt ttttaaatag gtttttagct gtttgattag tgagtgtaat aaaaattata
    41161 atatataaaa attatagaaa gtatatagac tattttagga ggaaatcagt atgaaatttt
    41221 atggatataa aagaccagat ggaagagttg gtataagaaa ccacatatta atattgccta
    41281 cgagtgtgtg tgcatcagat acaactagaa taatagcatc acaagttcaa ggggcagtta
    41341 cctttaataa tcagaatgga tgttcacaag tacattcaga ccaacaatta actatggatg
    41401 ttatggctgg ttatgcagca aatccaaatg tatatggtgt aatagttgta tctttgggat
    41461 gcgaaaattg ccaaaatgat ttggtagttg atgcaataaa agagagaaca aataagccaa
    41521 taaaaactct tgtaatacaa gaagaacatg gaactttaaa aactatagaa aaagctgttc
    41581 gttatgctag agaaatggca caagaagctt ctttgcttag aaaagaagaa tttccaatat
    41641 ctgaacttat actaggaact gaatgtggtg ggtctgaccc aacaagtgga ttagcagcaa
    41701 atccattaat aggtgaatta agtgataaac ttgtcgattt aggagcaaca tcaattttaa
    41761 gtgaaactac tgaatttata ggtgcagagc atatactagc taggagagca gtaaatgaag
    41821 aagtaaaaga aaaaatatta catatagtac atagatatga aaattcatta aaattggttg
    41881 gagaagaagt tagagaaggt aatccatctc ctggaaatat agctggtggt ttaacaagtt
    41941 tagaagaaaa atcacttggt tgtatacata aaggtggtca tagacaaata agtgaagtat
    42001 atgattatgc caaacaaatt gataagaaag gtcttgttat aatggatacc cctggaaatg
    42061 atgcttcatc tgttgctggt atggtagcag gaggagcaca aatagtggta tttagtacag
    42121 gaagaggaac accatcagga aatccaatag caccagtaat aaaaataact ggaaataaaa
    42181 taacttttgc aaatatggaa gataatctgg attttgatgc tagtccagtt atatatggtc
    42241 cacaaacaat ggaagaatta acagatgatt tgttaaatat ggttgtagat gtagcaaatg
    42301 gaaaacaatc aaaagcagag tcccttggat atactgaaat ggcaatagct agagtatgca
    42361 actatgtata atctttaagt aagaaaataa atacatagaa ttttaataaa gggtgagatt
    42421 atgaagtttg taagttttac tgaaggagaa aatgaatgta aaagaactgg agttttttct
    42481 aaagatgaat tatttattat agatgttaac tctttaaatc tcagtagaaa ttttaaggat
    42541 ttaaatgaat taattcaaaa tgtatctagt gaagatatta ttaagttgaa aactattata
    42601 aatgaaagta cattctataa ttatgatgtt tttaaaagag agagatgtac tgtacatgtt
    42661 cctatagaaa aaccaataca tgatatctta tgtgtgggag tgaactataa agaccatctt
    42721 gaagaaactc aaactcactt tgatgatagt tttgaagaac cacaaaaaac ggtttatttt
    42781 acaaaacgtg tgacaaaggc aataggtcca gaagatgaga tagatggtca ttttgagata
    42841 aataatcaac tagactatga agttgaactt ggagtagtaa taggtaaaac aggtgttaat
    42901 attagaaaag aagaggtaga agattatatc tttggttaca ctgttataaa tgatatttct
    42961 gctagaaagt tgcaaaaaga acatgttcaa tggtttagag gaaaaggatt agatacattt
    43021 acctctttag gtccatgtat tttacataaa agtgcagttc cttttcccat aaaacttaaa
    43081 gtatgtagta gggtaaatgg tgaagaaaga caaagttcta atacagggtt attcatatca
    43141 gacgtttcag acatagtttc agaactttca aaaggtatga ctttagaggc tggagacatt
    43201 atagctactg gaacaccttc tggagtaggg atgggatttt cacctcctcg atatatgagt
    43261 agtggagatg tagttgaatg tgagattgag agtattggaa ttttaaaaaa ttacattaag
    43321 taactgtttt aatataatta atattaaagc taaatctgat gtattaatga acatattaaa
    43381 ataaatttta ggagtttaaa ttatgaaagc agaatttttg acaccagttg ttacaatatt
    43441 tgatgaagaa ggaaatttag ataaagaagg taataaaaaa gtgtatgacc atcttataga
    43501 tggaggtgta gatggactag tcataatggg aagtactgga gagttcttta atatgtctat
    43561 ggatatgcaa aaagaactta tagatttagt aactagtcat gtaaataaga gagtaaaagt
    43621 gtttataggt acaagtcgta tgtgtataaa tgaatctata gaactttcaa actatgcaca
    43681 taatagtgga gcagatggtg ttatgataat aagcccttat tatttctctc ttgatgatga
    43741 tagtattgag ttattttata gtgaaatagc taagaataca gaggctgaaa tatatttgta
    43801 caattatcca gatagaactg gttatgattt aagtccagaa cttacattaa gacttgcaag
    43861 aaaacataaa aacatagttg gatataagga tacagtaact ttgatgggac ataccagaga
    43921 tttaataaat actatgaaaa tagagttccc agaatttaag gtttatagtg gttatgatga
    43981 aaactttata cataatctaa tgtctggggg agcaggttgt ataggggggt tatcaaattt
    44041 aattccagaa gtttgctcca aatgggctga agcttacaat aatagagata tggagaaggt
    44101 tatagaaatt caaaaatata ttaataaagc aatggatttt tacttaataa ccaatccatt
    44161 tataccagca atgaaaaaag ctatgaatat cagagggtta caaataagtg attatatgac
    44221 tgctccattt gtaaaaccaa atgaaaaaca agtatgtgaa attaaaaaat tgatgaatga
    44281 attaaatata tgttaaatca accttgcaaa gaggtcaaca ataaattaca attatcttaa
    44341 taaaaattaa attaaaataa tactagggta ctatattagt ttaataggga aagtgatgaa
    44401 aattcactac agcccccgct actgtatacg gatacgaaat caaaatccac tgaaatttat
    44461 aaaaattttg ggaagggtga tggaggaaaa gccgtgagtc aggagacctg cccagtatta
    44521 tataagttca atttagggtg gagattgtaa tttataataa aatgaagtat caattcgttt
    44581 ttgtaaagtt gatatatgaa cttctcctta ttaaattagg agaagttttt tattttatta
    44641 aaaggttata cttattaaag atgactttag gataacttta ataagtatac tttaaaaagc
    44701 atatattatt aaagtttttt agccactcat aaaaaataag atattggagt gaaagagatg
    44761 aaatttaaat caaatgtttt aaagcagttt tttttaattg ctatggtaat ttttatggta
    44821 ggaggtctta tagggtgtag taaaagtgac aaagagaata aacaagctag tactggtaag
    44881 aaattagaat taaaatatgc tagtggattt agtgttgaag atttagaaga tggaattaaa
    44941 aaagtaactg atggagataa tcgagaactg attttaatcc caaagaagat taaaattcct
    45001 gaaaaatata aaaatgctaa tatagttaga acgcctgtgg acaatgtatt aatagcatct
    45061 acaacacagg cttgttctct aagaacaata gatgaactag atagtataaa agccgtaaca
    45121 acacaagagc agtactggac tattaaagaa attaaagatt taatgaaaga tggaaaaata
    45181 cattacgttg gttcaaattt agcaccagat tatgaaaaaa tagtagactt aaatcctgac
    45241 ttaactatag tgagtagtgg tttatcagag gctgatacaa aatttattga caaattaaaa
    45301 gaattagggc ttaattatgt tgtagacaat gagtataaag agcaaaatcc atttggaaga
    45361 atggagtgga caagtttaat agctgcattt tatgataaag aagatatggc tactagtgaa
    45421 ctaaataaaa ctgtacaaaa ggtagaagaa gtaagtaaaa aaataaaaaa caaagctaaa
    45481 cctaaagttg tatgggcatc tgtatatgaa ggaagtgctt atatagctcc taaagattca
    45541 tatgtggata atatgataaa aatggctggt ggaataaatg catgtgcttc aatagattca
    45601 aatgaagggc gagtaagtat tgaacaattg catgaagttg caaaagatgc agatatattt
    45661 gtatactcat caagttcaga ttatgcacca aatttagatt ctataaaatc tagtgcacct
    45721 gtattggctg atttggatgt tgtaaaaaac gggaacctat gggtgtttgc tccagattat
    45781 tatcaatcag tagataagac agacgaatta atagtagatt tagttgagat gttccaccca
    45841 ggtactacag gtgaaaaaat caaacattat ataaattatg ataaatagta cagagaggac
    45901 acaaaaagat aggttttata gattttagtt aatatagctt tgctaaaagg agtatacaaa
    45961 tggattttat caataaaatg agaataagga atacgacaat atttatagta ttaattataa
    46021 gtatgatact tactttttta gtgtgtgtag catttggctc tgtaaaaatt gaatttaaag
    46081 aagtaataga tgttgtatta agaggtatgg ataatacaga acattataaa ataatataca
    46141 atataagaat ccctagagta cttgcaacca tggtaggtgg agcatgccta gcagtatcag
    46201 gtattttatt acagatattt tttaaaaacc caatagttga accgtatata ttaggagtat
    46261 cttcaggttc tactttatca gtagctctca tgattttggg aggatacaca ttgggatttg
    46321 agagtgtaaa ttcaatgagt atatttgtag cagcttttat aggagcttta attgtaatgt
    46381 ctatagtcat tttatttgca aataaggtaa agaatataac aactctttta ataataggaa
    46441 ttatgaccgg atatgtatgt agtggtataa caaatgtgtt acaatcattt gcaaatagtg
    46501 ataaattaaa agaatttact atgtggtcta tgggaagttt ttctggattt acatgggaaa
    46561 aggtagaaat acttataata gttggtatat taggtctttt gatgactgta ttcataataa
    46621 aacctttaaa tgctttttta ctaggagaag agtatgcaaa aagtattggc ataaatataa
    46681 gattttttag agttttgata gtatttatat ctagtatatt agcagcaata gtatcagcat
    46741 ttgcaggacc tatagggttt attggacttg cagtgccaca aatttcaaaa atgatattta
    46801 aaacatcaga taatagagtt ttgataccag tagtaatatt tttaggcagt atagttacag
    46861 ctctttgtga tttaatatca agagtaattt tatcaccagt agaattacca ataagtacaa
    46921 taacatcttt attaggagct ccaattgtag taatgttatt ggtgaaaagg aaggataggg
    46981 cttagacatg ttgaaaacta ataatttaag tgtaggatac aataacaaag ttgttataag
    47041 taatataaat gtagaagtaa aaaatggaga aatactatgt cttttgggaa gtaatggagc
    47101 tggaaaaact accctattaa ggtccttatc taaacttata tcaccaataa aaggtgaaat
    47161 atatttgaat ggtgtaaaca taaattgtat atcaagaaaa gcattatcaa aaaaaatggc
    47221 tttagtactt acaaatagat tattaggtga tttaatgact gtacaagata tagtcaatat
    47281 aggaaggtat ccatatacag gattttttgg gagtctatct aagaaagatt taataatggt
    47341 agatgaagca ttggagtctg tagatgcctt acatttaaag aaaagatatt ttgatgaact
    47401 aagtgatgga gaaaaacaaa aggtgttggt tgcaagggct cttgttcaag aaccagaaat
    47461 aattatatta gatgagccta caactcactt ggatataaaa catagattgg aactcataaa
    47521 catactgaaa aaattaagta aagaaaagtc aatatcagta atactttcat tgcatgaaat
    47581 agacatagca ttaaaaagtt gtgataaagt tgctcttata aaaaataata aagtcatagc
    47641 ttatggccag cctgaagatg tagtagatga ggatattata aattcgcttt atgaattgga
    47701 tgataaaaac tttaatagtc ttttaggttc agtagaaatt agtaataaat ctaaaaatga
    47761 agtttttata attggtggtg ggggtaaagc aacaccaatt tatagagctt ttactaaaaa
    47821 aggtataggt ctgtattctg gaataataca tgaaaatgat attgattatg aaattggtag
    47881 aactatgggg ataaagatgt ttacagaaaa tccatttgag cctataagtg atgagagttt
    47941 cgatttggct attagaaatt taaatgattc caagattata atagatacag gatttagtgt
    48001 aggagagaca aataagagga atatagatat tataaaagaa gctttaaaat tggataaaaa
    48061 agtttattct tttagaaata gagatgagag taagaagtat tatgacagtt tggatagtaa
    48121 gatagaacat atagataaag tttctcaaat aatcaatagt gcagatataa ataatttatt
    48181 ataatttatg aaataaaatg tacttatgtg ttatagaaaa aagagtatag attataattt
    48241 ttttgaaatt acaatctata ctcttttgtt attttgttta tgtatttata tatttataaa
    48301 actttagcat tttatagata accaatagct tcctctctag ttaaaaaaaa gtgaatccct
    48361 gtagtcgatt ctttccatct attttcatta aaatcttcag ctacaatcgt ttctcctact
    48421 ctatatataa aattttcatc agcatatgca cgagcacttt taaaagattc tttaccatct
    48481 acactagtaa ttgacagtac ttttgcctta tcacatctac aagcatttga tgtagcagac
    48541 actctttttg cgtcgctggg tattaataat tgtaccattc taaaattaaa acatttttta
    48601 tagcctataa atgctcctgt ttcaggacaa tacaatttaa aaaaacgagt actttcatct
    48661 gatattactc catttaaagt agcatattct aaattggaat tttctaaatt agctcctcta
    48721 atatctgagt tagataagtc acaaaatcta aaacttgcac cacttaagtc tgcatttttt
    48781 aatatagaat tttttaatga acaatccgaa agataagaat tattaaaaat agagttttct
    48841 aaatctgctt tttctaaatt taaattgata aggtcagacc acgaaaagtc aatattactt
    48901 aagcagtatc ctcttaagtc catattttct aatcttaatt cttttaaatc tgccttttct
    48961 ccagcattat tgtttttaag ccataaataa tgtttctcaa gtgttttttc taattcttgt
    49021 ttagaaatag ttttcattaa aatcaccttc ataaatttat ttttatatat gtagtagcaa
    49081 aatagtcata tatttaatta tataacaata aataaaaa
//
LOCUS       DAMJZE010000010        48782 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJZE010000010 DAMJZE010000000
VERSION     DAMJZE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.010"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            8..1204
                     /locus_tag="PC541_000483"
     CDS             8..1204
                     /locus_tag="PC541_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2837963.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1297..1821
                     /locus_tag="PC541_000484"
     CDS             1297..1821
                     /locus_tag="PC541_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2837964.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1846..2022
                     /gene="rpmF"
                     /locus_tag="PC541_000485"
     CDS             1846..2022
                     /gene="rpmF"
                     /locus_tag="PC541_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2837965.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2229..2786
                     /gene="fapR"
                     /locus_tag="PC541_000486"
     CDS             2229..2786
                     /gene="fapR"
                     /locus_tag="PC541_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2837966.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2797..3819
                     /gene="plsX"
                     /locus_tag="PC541_000487"
     CDS             2797..3819
                     /gene="plsX"
                     /locus_tag="PC541_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2837967.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3845..4831
                     /locus_tag="PC541_000488"
     CDS             3845..4831
                     /locus_tag="PC541_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2837968.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4940..5869
                     /gene="fabK"
                     /locus_tag="PC541_000489"
     CDS             4940..5869
                     /gene="fabK"
                     /locus_tag="PC541_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2837969.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5885..6835
                     /gene="fabD"
                     /locus_tag="PC541_000490"
     CDS             5885..6835
                     /gene="fabD"
                     /locus_tag="PC541_000490"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2837970.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6835..7584
                     /gene="fabG"
                     /locus_tag="PC541_000491"
     CDS             6835..7584
                     /gene="fabG"
                     /locus_tag="PC541_000491"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2837971.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7638..7862
                     /gene="acpP"
                     /locus_tag="PC541_000492"
     CDS             7638..7862
                     /gene="acpP"
                     /locus_tag="PC541_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2837972.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7891..9129
                     /gene="fabF"
                     /locus_tag="PC541_000493"
     CDS             7891..9129
                     /gene="fabF"
                     /locus_tag="PC541_000493"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2837973.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9355..11073
                     /locus_tag="PC541_000494"
     CDS             9355..11073
                     /locus_tag="PC541_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2837974.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11104..13104
                     /locus_tag="PC541_000495"
     CDS             11104..13104
                     /locus_tag="PC541_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2837975.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13455..13700
                     /locus_tag="PC541_000496"
     CDS             13455..13700
                     /locus_tag="PC541_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2837976.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13735..14460
                     /locus_tag="PC541_000497"
     CDS             13735..14460
                     /locus_tag="PC541_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2837977.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14482..15795
                     /locus_tag="PC541_000498"
     CDS             14482..15795
                     /locus_tag="PC541_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2837978.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16001..16540
                     /locus_tag="PC541_000499"
     CDS             16001..16540
                     /locus_tag="PC541_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2837979.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16629..18614
                     /locus_tag="PC541_000500"
     CDS             16629..18614
                     /locus_tag="PC541_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2837980.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18838..19806
                     /gene="spoIIIAA"
                     /locus_tag="PC541_000501"
     CDS             18838..19806
                     /gene="spoIIIAA"
                     /locus_tag="PC541_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2837981.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19796..20317
                     /locus_tag="PC541_000502"
     CDS             19796..20317
                     /locus_tag="PC541_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2837982.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20334..20531
                     /gene="spoIIIAC"
                     /locus_tag="PC541_000503"
     CDS             20334..20531
                     /gene="spoIIIAC"
                     /locus_tag="PC541_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2837983.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20547..20933
                     /gene="spoIIIAD"
                     /locus_tag="PC541_000504"
     CDS             20547..20933
                     /gene="spoIIIAD"
                     /locus_tag="PC541_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2837984.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21018..22223
                     /gene="spoIIIAE"
                     /locus_tag="PC541_000505"
     CDS             21018..22223
                     /gene="spoIIIAE"
                     /locus_tag="PC541_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2837985.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22283..22900
                     /locus_tag="PC541_000506"
     CDS             22283..22900
                     /locus_tag="PC541_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2837986.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22912..23466
                     /locus_tag="PC541_000507"
     CDS             22912..23466
                     /locus_tag="PC541_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2837987.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23493..24182
                     /locus_tag="PC541_000508"
     CDS             23493..24182
                     /locus_tag="PC541_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2837988.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24292..24654
                     /locus_tag="PC541_000509"
     CDS             24292..24654
                     /locus_tag="PC541_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2837989.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24739..25245
                     /gene="nusB"
                     /locus_tag="PC541_000510"
     CDS             24739..25245
                     /gene="nusB"
                     /locus_tag="PC541_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2837990.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25229..26299
                     /locus_tag="PC541_000511"
     CDS             25229..26299
                     /locus_tag="PC541_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2837991.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26296..27501
                     /locus_tag="PC541_000512"
     CDS             26296..27501
                     /locus_tag="PC541_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2837992.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27503..27718
                     /gene="xseB"
                     /locus_tag="PC541_000513"
     CDS             27503..27718
                     /gene="xseB"
                     /locus_tag="PC541_000513"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2837993.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27721..28608
                     /locus_tag="PC541_000514"
     CDS             27721..28608
                     /locus_tag="PC541_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2837994.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28638..29075
                     /locus_tag="PC541_000515"
     CDS             28638..29075
                     /locus_tag="PC541_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2837995.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29109..30974
                     /locus_tag="PC541_000516"
     CDS             29109..30974
                     /locus_tag="PC541_000516"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2837996.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31107..31919
                     /locus_tag="PC541_000517"
     CDS             31107..31919
                     /locus_tag="PC541_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2837997.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31939..33618
                     /gene="recN"
                     /locus_tag="PC541_000518"
     CDS             31939..33618
                     /gene="recN"
                     /locus_tag="PC541_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2837998.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33832..34698
                     /locus_tag="PC541_000519"
     CDS             33832..34698
                     /locus_tag="PC541_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2837999.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34791..35282)
                     /locus_tag="PC541_000520"
     CDS             complement(34791..35282)
                     /locus_tag="PC541_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2838000.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35343..35783)
                     /locus_tag="PC541_000521"
     CDS             complement(35343..35783)
                     /locus_tag="PC541_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2838001.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35944..37002
                     /locus_tag="PC541_000522"
     CDS             35944..37002
                     /locus_tag="PC541_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2838002.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37144..37968
                     /gene="spo0A"
                     /locus_tag="PC541_000523"
     CDS             37144..37968
                     /gene="spo0A"
                     /locus_tag="PC541_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2838003.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38381..39502
                     /locus_tag="PC541_000524"
     CDS             38381..39502
                     /locus_tag="PC541_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838004.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39520..40395
                     /locus_tag="PC541_000525"
     CDS             39520..40395
                     /locus_tag="PC541_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2838005.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40419..41111
                     /locus_tag="PC541_000526"
     CDS             40419..41111
                     /locus_tag="PC541_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2838006.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41119..41943
                     /locus_tag="PC541_000527"
     CDS             41119..41943
                     /locus_tag="PC541_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2838007.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41948..43015
                     /locus_tag="PC541_000528"
     CDS             41948..43015
                     /locus_tag="PC541_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2838008.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43017..43553
                     /locus_tag="PC541_000529"
     CDS             43017..43553
                     /locus_tag="PC541_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2838009.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43621..44226
                     /locus_tag="PC541_000530"
     CDS             43621..44226
                     /locus_tag="PC541_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2838010.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44238..45152
                     /locus_tag="PC541_000531"
     CDS             44238..45152
                     /locus_tag="PC541_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2838011.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45194..46366
                     /locus_tag="PC541_000532"
     CDS             45194..46366
                     /locus_tag="PC541_000532"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2838012.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46413..47225
                     /locus_tag="PC541_000533"
     CDS             46413..47225
                     /locus_tag="PC541_000533"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2838013.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47429..48754
                     /locus_tag="PC541_000534"
     CDS             47429..48754
                     /locus_tag="PC541_000534"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2838014.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        19722 a         4913 c         9179 g        14968 t
ORIGIN      
        1 aataataatg aaaatattag tattaaactg tgggagttct tcattaaaat atcaattaat
       61 agatatgaat aatgaagaag ttttatgtat aggattggtt gaaagaatag gaatagaagg
      121 ttctatatta aaacatgaaa aagcaggtag agatgataaa tacgttgttg aacaaccaat
      181 gaaggaccac aaagatgcaa tagcattagt tttagaagct gttgctcatc cagaatttgg
      241 tgcagttaaa gaaatgaaag aaatagatgc agtaggacat agggttgtac atgctggaga
      301 aaaatttgca acttcagtag taataactcc agaagtagaa gaagctttaa aagaatgtat
      361 agatttagct ccacttcata atccagcaaa cataatggga atagatgctt gtaaagctat
      421 acttccagat gtaccaatgg taggtgtatt tgatactgct ttccatcaaa caatgcctaa
      481 atcttcatac ttatatggtt tacctcatga gctatatact aaatatgggg taagaagata
      541 tggattccac ggaacttctc ataattatgt atctcaaaga gcagcagaga tattaggaaa
      601 agatataaaa gatttaaaaa tagtaacttg tcacttagga aatggtgctt ctatagctgc
      661 tgttgatggt ggaaaatgtg tagatacatc tatgggattc actccattag aaggattaat
      721 aatgggaact agatgtggag atatagaccc agctatatta ccattcttaa tgagaaaaga
      781 aggtttagat gctgatggat tggataaatt aatgaataaa gaatctggag tatatggaat
      841 gactggaatt tctagtgact tcagagatat agaagatgca gctaaaaatg gagacgaaag
      901 agctcaagct acattagaag cgtatgttaa aaaagtacaa aaatatatag gcgcttatgc
      961 tgctgaaatg aatggattgg atgttgtagt atttactgca ggcgttggtg aaaatggaaa
     1021 ggctataaga gcagatattg cttctaacat ggagttttta ggaatgaaat tagataaaga
     1081 agctaatgat gttagaggaa aagaaacagt aatatctact gctgattcta aagtaaaaat
     1141 gcttttaata ccaactaatg aagaattaat gatagctaga gatacattaa gattggtaaa
     1201 ataattaaat taaacctatg cctatcaagt aaaaatactt gacaaacaaa tgctagattt
     1261 gtataataaa tttggtgata tgttgaggtg aacttaatga aagttagcat agaaaaaata
     1321 aatagaaaag aaactgacaa aatagacttg aatttttgtg aaaaaattga tactattagt
     1381 tattgtgatg agatttacaa attagtatca cctgtaaatt taaaagggaa agtgtcaaag
     1441 actaataaag gtttgtatct agatatagat gtcaatttta caattgttga caattgttca
     1501 agatgtctta aagaagtaga aataccacta gagtattcta tacaaggttt tttagtaaaa
     1561 gaagaagatt atgatgaaga tgagtttgag gaatttgatc cttttatatt cgatggtgaa
     1621 gaaatcgacc ttattgatat aatagaacag acattagatt ttaatgtacc tcataaagtt
     1681 ctttgtagtg aaaactgtaa aggtctttgt caagtatgcg gggcaaactt aaatgaagaa
     1741 gagtgttctt gcagtgaaat tacaaacgat gaggaataca tagatcctcg ttttgctaaa
     1801 ttaaaagatt tatttaatta aattttgact aaggaggtgg cgtaaatggc agtaccaaag
     1861 cgtaaaacgt ctaaatcaaa cacaaaaatg agaagagcag ctaactcaaa gatggaagca
     1921 acaggatttg taagttgccc acaatgtcat gagccaaaat taccacatag agtgtgtcca
     1981 gactgtggat attataaagg taaagaagtt gtgtctaagt aatagcttct aaaaagcatg
     2041 tagagtatac tctacatgtt ttttttgtac atataaaaaa ataagataat ttactagaga
     2101 aaataagtgg attttatgca tattaaaaaa tagaaaaata ataatataag tattgaaaat
     2161 ttttgtgtgt tactatatac tactattagt agctggtact aattacagct ataaaatttg
     2221 gagggaacat gaaaaagaaa agtaaagccc aaagacaaaa agagcttata gatatgctaa
     2281 agacagatcc tttttatact gatgaagaat tatcaagtct gtttgatgta agtatacaga
     2341 ccataagatt agatagaatg agtctaaaca taccagagct aagagaaaga gtcaaatcaa
     2401 tagcagaaac tcagagttca aaagtaaaga ctttaggagt aaaggaaatt acaggagaaa
     2461 tcattgattt atctgtaggg cgattaggta tatctatgtt agaagttaca caggacatga
     2521 tttactctaa gactaatact ttaaaagata cgtatatttt ttctctagca gattcattgg
     2581 caatggctat aatagatgca cctaaggtta ttatgagagt tgcaaatgta aaaagtttta
     2641 agttgataga acaacaagat aggcttatag caaaagcaga agtgtataga aatatagata
     2701 aaaagcatta tgtaaaagtt gttatcaaca ataaagccca agagcaaata tttagaggaa
     2761 agtttatctt tgaagaatta gattaggagg gaaattatga aaatagtaat tgatggaatg
     2821 ggtggagata atgcacctaa atctaatgta gaaggcgcag taaatgctat aaaagaatat
     2881 caggttgatt taataatcac aggagataaa gatttacttg aaaaggaatt ttcaaattat
     2941 gaatttgata gaaataaatt agagatagtt catactactg agattataga aaacgaagat
     3001 aaaccagtaa aggctataag aagtaaaaaa gattcatcta tggtagtagc attaaatctt
     3061 gtaaaagaag gaaaggcaga tgctataata tcagctggaa atacaggagc cttacttgct
     3121 ggtggattat ttgttgttgg tagaataaaa ggaatcgata gaccttgctt atgttcagca
     3181 ataccaaatg taaagcgagg aatgacactt atagcagact gtggcgcaaa tgcagattgt
     3241 aaacctaaga atctagttga atttgcagct atgagtaata tttattcaag aaaagtttta
     3301 ggtcttgaaa atcctaaggt agcattagca aatgttggac tagaagaagg caaaggcaat
     3361 gatttagtca aaaggtcata tgaagaaatt aaaaaattag atttaaattt tatagggaat
     3421 gtagaagcta gagaagttat aaatgcatac acagatataa tcatatgtga tggatttaca
     3481 ggtaatatat tacttaaatc tgctgaaggt gtagcacttt cagttatgag tcttataaag
     3541 gaaactttta tggccagtac aaaaagtaaa ataggagcat tacttataaa ggatgattta
     3601 agaaaattaa aaagttttat agattattct gagtatgggg gagctccact tttaggtcta
     3661 aatggaggag ttataaaagc tcatggtagt tctgatgcta aagctataaa aaatgctata
     3721 aatcaaggaa taaaattttc aaagggaaaa gtcgttgaag atattaacca atttataagc
     3781 aaatacaatg aagaaaataa aaataacgaa gatgaataaa ctcgttttgg aggtttaata
     3841 tataatgaat acaaaggctg gaatattagg agtaggaagt tatctacctg agcaatcata
     3901 tgataatttt cacttcgaaa aaataatgga tacatcagat gagtggataa gtactagaac
     3961 tggtattaag gaaagaagat ttgccaaaga gtctgaagca acttctgact tagcatctaa
     4021 agctgcctta aaagctatag aatgtgcaaa gttaaacgta gaggatatag agctgattat
     4081 attagctaca attactcctg atatgtcttt gccttcaact gcatgtatag tacaagatgc
     4141 aataggagca gtaaatgcta ctgcatttga tatatcagca gcttgttctg gatttgtata
     4201 tggagtaact attgcaaagc aatttgtgga aacaggttgc tataaaaatg tgcttgtaat
     4261 aggagcagaa acttgttcta agtttttaaa ttatgatgat agaacaacag cagtattgtt
     4321 tggagatgga gctggagcag ctgttattgg ccctgtaaat gagggtggaa tactgtctac
     4381 acatatgggc tctgatggta aaggaaaaga ttgtttaaaa gtacctgctg gaggttcaag
     4441 acttaaggca agtaaagaga ctgtagaagc taatctacat acaatagaaa tggcaggtag
     4501 tgatgtgttt aaatttgcag ttagaaagat ggcagaaact tctttaagag ctcttgaaaa
     4561 agcaaatcta aatacaactg atatagacta cttagtgcca catcaagcta atataagaat
     4621 catacaagct tcttctaaga gattagagct tgatatgaaa aaagtttatg taaatataga
     4681 caaatatgga aatatgtctg ctgcatctat accagtagca ttagatgaag cttatagaga
     4741 aggtaaaatt aaaaagggtg ataatgtagt tttagttggc tttggtggag gtctaacttg
     4801 gggggcttca gttgtcaagt ggactttata gtataaaata tctaaaatga tttaattaac
     4861 aaaattaaac ttatacacaa aataaagttg attaataata aaaagtttac ttttatgatt
     4921 tcaagtgaag gagaattgta tgaataaaat ttgcaaaata ttaaatataa aatacccagt
     4981 tatccaaggg ggaatggcat gggtagctac tgcatcatta gcaagtgctg tatctaatgc
     5041 aggaggactt ggcataatag cagcaggaaa tgcaccaaaa gaagctataa agaaagaaat
     5101 tgttgagtgt aaaaaattaa cagataagcc ttttggagta aatgtaatgc ttatgtcgcc
     5161 atttgttgat gatataattg atttgattat agaagaaaaa gttcaagtta ttactactgg
     5221 tgctggaaat cctgcaaagt atatggatag attaaaggaa gctggaacaa aggttattcc
     5281 tgtagtacct acaatagctt tggcacaaag aatggaaaag ctaggagcta cagcagtaat
     5341 agcagaaggt actgaaggtg gaggacatat aggagaactt actactatgg tcttagttcc
     5401 acaagttgct gatgctgtaa acatacctgt aatagctgct ggaggaattg tagatggtag
     5461 aggaattgca gcatcatttg cattaggtgc cagtgcagtt caagtaggaa ctagatttat
     5521 ttgcagtgaa gagtgttctg tccattcaaa ctataaaaac ttagtactaa aagcaaaaga
     5581 tagagatgca attgtaacag gaagaagtac tggtcatcca gtaagaacat taaaaaataa
     5641 actatcaaaa gaatttttaa agatggaaca aaatggagct actcctgaag aactggataa
     5701 aaaaggtaca ggagctttaa gatttgcaac agtagatgga gacatagaaa aaggttcatt
     5761 tatggcaggt caaagtgctg ctatggtaaa agaaataaca ccttgtaagg aaattataga
     5821 ggctatggta aatcaagcaa gagagattat gccagcaata gaactgtaaa tcaggaggag
     5881 caagatggga aaagttgcac tagtattccc tggtcaagga gctcaatatg taggtatggc
     5941 caaggattta tatgaaaata atgctgttgc taaaagtgta atagatgagg ctagtgatgc
     6001 cttaaatatg gatttaaaaa accttatgtt taatggaaat gaagaagaac tttctaaaac
     6061 tgaaaacact caacctgcta tggttacaca tagtgtagca gtattaaaag cggtacaagc
     6121 tcaaatagat ttaaaatata atgcatgctt aggattatcc ttaggagagt atagtgcgtt
     6181 ggttgcagca gatgcaattg actttaaaga tgctgtatgt ttagttaaaa aaagaggtaa
     6241 gtttatgcag gaaacagttc cagcaggtgt tggagctatg gcagcagttt taggtttaga
     6301 tagaaatgta cttgaaaatg tggttaaaga tatacaagga ggtattgtag aagtagcaaa
     6361 ttacaattct cctggacaaa tagttatatc tggtgaaaat gaaaagatag aagaagcaat
     6421 ggtaaaatgc aaagaagcag gagcaaaaag ggcagttaaa ttaaatgtaa gtggaccttt
     6481 tcactcatca atgttaaaag aagctggtgt aaaattggcc agtgaattag aaaaagttaa
     6541 cataactaaa cctaaagttg atgtggtggc aaatgtaaat gcagattact ataaaaacga
     6601 aggtaaagac ctgcttataa aacaagtaag ttcatctgtt ctttgggaag attctataga
     6661 aagactttta aatgatggat atgatacttt tatagaaatg ggacctggaa aaactttaaa
     6721 agcatttatt aagaaaatag cttccaataa aaaagcaaat gtcaatattt ataatataga
     6781 tggtatagat tctcttaatg aatttgtaca aaattataga aatggagaga tataatgata
     6841 aatcttacag gacaagtagc agttgttact ggtggttcta ggggtatagg aaaagaaata
     6901 gcaaaaaaac tagcatcttt tggagctgat gtagtaatca attatacttc taaagaagat
     6961 gaagcactaa aaactaaaaa tgaaatagaa agtatggggg taaagtgtac ctctataaaa
     7021 tgtgatgtgt ctaaatttga tgaagtgaat caaatgatag attctgttgt aagcgaattt
     7081 ggaaaaattg atatattggt taataatgca ggcataacta aagatggtct gcttatgaga
     7141 atgaaagaag aagattttga tagagttata gatataaact taaaaggtgt ctttaattgt
     7201 acaaaagcag ttactaaacc tatgatgaaa aagaagtatg gaagaataat aaatatgact
     7261 tcagtagttg gaattatggg taatgcaggg caaactaatt attgtgcatc aaaagcaggt
     7321 gtaattggat ttacaaaagc ttctgcaaga gagttagcat caagaaacat aaatataaat
     7381 gcagtagcac ctggatttat agaaacagat atgactaaag tactaagtga tgatgtaaaa
     7441 gaatcaacac tagcaaacat accaaagaaa tcttatggta aaccagaaga tgtagccaat
     7501 gccgtagcat ttttagttag tgacatgtca agttatataa caggacaagt aataaatgta
     7561 gatggtggaa tggtaatgca ataataattt aaaaagtaga gaaatctatt aaaataaaaa
     7621 attttaggag gtacacaatg tttaataaag ttgtagaaat aataatagag caattaggag
     7681 tagaagataa agaaataact atggaaacat cattaatgaa ggatttagaa gcagattcat
     7741 tagatgcagt agaaataata atggcacttg aagatgaatt tggtatagaa ataccagata
     7801 cagaagcaga aaacttcaaa tctataggag atatagttaa gtatatagaa gctaataaat
     7861 agtcgagctt taaaaaagtg gaggtatccg atgaataaaa gagtagtaat aacaggactt
     7921 ggatgtgtta cgccgttggg aacaggaaaa gaagagttct ggagtaatat aaaatctgga
     7981 gtatctggta tagataaaat aactaatttt gatgctagca catatcaaac tcagatagca
     8041 ggagaagtta agaattttca tccagaagaa tatataagta aaaaggaatt gaagagatta
     8101 gataaatttg ctcaatttgc aatagtatct gctaaattag cagttgaaga tgcaaatctt
     8161 gatttagata aagttgatag agaacgtttt ggtgttataa taggttctgg aataggtgga
     8221 gttgaagcta tagaaactca acataaaatt cttttggaaa aaggaaataa aagagtaagt
     8281 tcactttttg tacctatgat gataggaaat atggcagctg gtcaagtttc aatattttta
     8341 ggagcaaaag gacctaacac taatgtatgt acagcttgtg catcaggaac tcattcaata
     8401 ggtgatgctt ttaaggtaat tcaaagaggt gatgcagacc ttatggtagc agggggttct
     8461 gaagctgctg taacaggtct tgcttttgct ggattctgta acatgaaggc tatgtcaact
     8521 agaaatgatg accctaaaac tgcatcaaga ccatttgata aagacagaga tggttttgta
     8581 atgggagaag gagcaggtat tgttatctta gaagacttag aacatgcttt agcaagaggt
     8641 gctaaaatat atgcagaagt agttggatat ggtttaactg ctgatgcata ccatatgaca
     8701 actccagctg aaaatggaga gggagcagct aggtctatga atatggcttt gaaagatgga
     8761 aatgttccat tagaagaagt agattatata aatgctcatg gaacttcaac ttattacaat
     8821 gatttatatg aaactatggc tataaaaact gtatttggag aaaaggcata tgacttatgt
     8881 gtgtcatcaa caaaatctat gactggtcac ttattaggag catcaggtgc tatagaggct
     8941 gttgtttgtg ctatgagtat agaagatagt tttgtgcctc caactataaa catacaagaa
     9001 gttggtgaag atttggattt agattatgtt ccaaatcaag gtaaagaaaa aaatattaga
     9061 tatgcactat caaattcttt aggatttggt gggcataatg ctacaatagt actaaaaaaa
     9121 tatgtataaa taaatattaa atataaaact aaaaaacaag cacttgtttt aactgtataa
     9181 atttatatta gcttaaattt aaagtctcct aaattatttt aggagatttt tttattaaga
     9241 tttaagtcta ggatatgttg taaatcattt tgatatattg tattattaat tattaagagg
     9301 ttatttatat atgcaagttt tcattctaca atatacaaaa ggaggactaa aattttgaat
     9361 tttagactac caaagaaaag gttacccaaa aagataattt ttatatgtat aatacttatt
     9421 agtattattt tgtttataaa gtatgaccaa aatggattga aagaaagaga aattataaat
     9481 aagggaaaat atatgtctga aagccttgtg gcagataagg agtatgatga agcaaaaaaa
     9541 gtggttaaga cttcttttga atctattcca gaaaaaaaat acaataaagt atataatgaa
     9601 gtttgggata tgttaaagac tatttcatat gttccagatg gaacgaagat agtcattgat
     9661 tctttagaaa agatgagaga atcagatgtg aaattatctg atgaatgtag atttaatata
     9721 gaaaaaagat tagcatcagt gtatataatg gataataatt attctaaggc agtagattta
     9781 actgtaaagt ctataaagtt ggctgagaaa ttggggaata attatgaaaa agcaagacta
     9841 gatgttgatt tggcgagcat atttttaaag ataggtggct atgaaacagg agagaaatta
     9901 ataaaggatt cttttaaaat agatattgat gaaggtgaaa aaaatggaat ggttagatta
     9961 tatgctatta taaatctagc agaaatatat gtagaagagg gcgaatatga taaagctatt
    10021 gaaataagta gcaggataaa agattataaa aagtttgtaa atacggataa ttacaatgat
    10081 tttgatataa tggcctctat aatgcagtca aatgcgtatg tgggcaaaaa taatatacaa
    10141 aaggcaaaga cttttttaga aaaagctgat tatcttataa aatcagatag aacagtttat
    10201 atattagata aagatatgta ctattatatg gcaatgggaa acttagaata taaaagcgaa
    10261 aattataatt ctgcaataga taattataaa aaaagtttag agatatctac taaaagaaag
    10321 ttaacccagg agaaaataaa aaatttaaat aagatattaa atatatatga aaaacaagga
    10381 aatatagaat ctttaaatga gtatcaaaag atgttaataa atgaatataa agagaacaaa
    10441 agtataagag attcagagac tagtttctat ataattgata aagtatcaaa cgaaagtaag
    10501 ctatttgaaa aaactaaaaa agaaataaaa tattataaaa tattatttac agtaatttta
    10561 attgctatat tagggtctac ttttttatac aaccgattga agtattttaa agcacaaaat
    10621 ttacatgatg gtcttacaaa tgtttataat agaaaaagtt ttgatattat gtatgaaaag
    10681 tacagagaga aagatgataa ttttgcactt gtgatgatag atattgataa tttcaagctc
    10741 ataaatgata attatggaca taaatttggt gacactgtta taaagggaat aacaaatacc
    10801 atttgtgtta tgttagaaca agatgataaa gtctttaggt acggaggaga agaattttct
    10861 gtattaataa gaaataaatc tggtgatgaa gtagcagata taattgacag tataagggtt
    10921 gcaattgcaa ataaaaagtg gaatgatgat gtgactgtga ctataagtgc aggtgtagct
    10981 catgtttcag atagcaaaga tgtactagaa gaagctgata aaaatttata taaagcaaaa
    11041 caacttggta gaaataaagt agtatataag taattatata ctatataaat aagaggtata
    11101 aatatgaaac aatcaatagc tatagtaact gataaaataa ctaaactatc atctttctta
    11161 gaagaaaata tacgtttggt tttaggtaat tatattgata taaaccacta ttatctggaa
    11221 gttttaaaag agaatgataa aatacaaggt gattttgtac tagtaatgaa tgatgataga
    11281 ttaaacagta tgagaaagca tttagtacaa gatagtaaga ttatagtgat aaggagaaca
    11341 cttaaagaaa atgaaatata tggattattt tcaataccaa atggaactga agtattggtt
    11401 gtaaatgata caaaagaaac taccttagaa acgataagct tattttacaa aataggagta
    11461 aaaaatttaa agtttactcc atatattgaa ggaaaaattt ataaaaatat agacatagct
    11521 ataacacctg gagtaaaggg aaaagtacca agttatataa ataaagtaat cgaccttgga
    11581 aatagatata ttgatatatc tacctttata gaaataataa atggtcttag aatagatgtt
    11641 aaagaaatac gtaaaaattt aatggcatat tctgagagtt taataagttt agataatgga
    11701 gttaaagata attataggca actgttttta aaaatagaag aacaagatgt tatattgaat
    11761 ctatcaaaag atggaataat atttacttca gcagatggta taataaacac atttaataaa
    11821 gaagctttaa aaattttagg tataaatgaa aatccacaag gcaaaaacat agaagatatc
    11881 atttctgaag atttaaatat tttacttgaa gacaaagaaa taattgatga agtggtattt
    11941 acaaataaaa aatatataaa tgtaaataaa aaaagtattt tttctatggg gaataaagct
    12001 gggatatatt ataacttaca agaaataacg tatataaaaa aattagaaca aaatcttact
    12061 aataaattaa gagaacaagg tcagatagca agatatacat ttaaagatat aaaaactaaa
    12121 aataaaaata tggtaaagtg tgtagaattg gctaaaaaaa tatctaaatc agacctttct
    12181 gtattaataa taggagaaag tggtactggt aaagaattat tatcacaatc aattcataat
    12241 gcttccaata gaagtaatca accgtttata gcagttaatt gtgcagcagt tccagatagt
    12301 ctattagaaa gtcaactgtt tggatatgaa aaaggctctt ttacaggagc tttaagagaa
    12361 ggaaaaaagg gtctatttga acttgctaat aatggaacta tatttctgga tgaaattgga
    12421 gatatgcctc cattgctaca gacaaagtta ctaagggtat tacaagaaaa gcaggttatg
    12481 cctataggtt cccataatgt tataaacata gatgttaggg tcatagcagc aacaaacaaa
    12541 aacttactaa agatgattag agaaggaaaa tttagagagg acctgtatta tagactaaat
    12601 gtattgccaa tagatgtacc atcattgagg aatagaaaag aagatataat aacacttatg
    12661 aatttctttt tgaaaaacaa tataagactt tcgccagaag ttataactat tttagaaaaa
    12721 tataattggc caggaaatat aagagagtta caaaatgttg cttcttatgt aagtctaatg
    12781 tgtgatagta tggtttttag agatgactta ccaccatata taagggtaga atgtgctaac
    12841 aattatgaag atggatttgg ttatacaaga aaagaatttg aggaaatact gaggattcta
    12901 tatgaaagaa gagagtcatc ctctggaatt ggaagagggt ttataatcga aaagcttata
    12961 caggaaggta ttgagatttc tgaaagcaaa gttagaaagg tgctatctta tttaagtgaa
    13021 tgtggataca tatcatctaa atctggtaga ggaggaagtc aaataacctc agaggggata
    13081 aaactttata ataatataaa ttaagctaag tatttgatat tgaaatcaca tattttgaat
    13141 ttagatattt aaataataat aaacttttat atttatagag attaatttat attatgtcag
    13201 aaagaatatt gtaatttata attaaatatt aataggtttt taaaatagga ttagataggt
    13261 aataggttaa gttagaataa cccattacct atttttattt gtaagaaaag tgtacataga
    13321 attagaagta aaaaatacaa ctaataaaac tattgaaaat actagataaa taataaataa
    13381 aaataattat agattttggc atacatattg ctaattatag agataagacg acaaaataat
    13441 tgggaggttt aatgatgaaa tacaaaacaa cagggacttg tgctacagaa attgaatttg
    13501 aagtaaaaga aaataaagtt acaaatgtaa actttatagg tggatgtgat ggaaatttaa
    13561 aagggctaaa agtattagtt gaaggcatga atatagaaga tgtaatacaa aagttaaaag
    13621 gaatagagtg taaaacaaag ccaacatctt gcccagacca actgtctcta gcattggaaa
    13681 attatatgaa tataaagtaa tacataaaac aatcgattag ttaggagttt aattatgaaa
    13741 ccaataatag ggattttagg aaatttaata ataatggaaa atggtatgtt tccaggtctt
    13801 gaaaggtcat atgtaaataa tgattatata aatgcagtgc taaaaggtgg cggtagtcca
    13861 gtaataattc ctgtaaatac agataaagaa gttataaaaa aacagattga aatggttgat
    13921 ggagtcttaa tatctggggg atgggatatc aatcctcaac tgtatggaga agaaacaaga
    13981 gaagaaacga catttatata tccagaagtg gatgaatttg atttaattgc aataagtata
    14041 gcattagaac ttaaaaagcc tatattaggt gtatgtaggg gactacaaat tttaaatgta
    14101 agtttaggag gaactttata tcaagataat aatcttatag aaggaagtta tataaaacat
    14161 acacaatctt ctaaaagaca tgtagctact cataaagttg atgttaaaga aggaagtatt
    14221 ttagagggaa ttttaggaaa acaattactt acaaatagtt atcatcatca aagtgtcaat
    14281 cagcttggaa aaggattaaa agccatagca tactcaaaag atggtataat agaagctatt
    14341 gagaaagagg atgaaaattt tgtagtagga gtacagtggc atccagaaat gatggtagat
    14401 tactgtgata aaatggaaaa actatttaaa tattttataa atatttgttc gaatttataa
    14461 tataccttgg ggggattgaa catgaacgaa aagaacaaaa tgggattaat aagtattata
    14521 cttttaggta ttaatgcagt agtaggagct ggagtttttc tattaccagg agatgcaatg
    14581 aaatcatttg gtgtagcaag tatatttgta tatatatttg atatgttgtt agttttatct
    14641 atggcattct gttttgctga ggttgcaggt aaattcaata aaaatggtgc tgcctatgta
    14701 tatacaaaag aagcttttgg agatttttgt ggttttgaag taggtcttat gaaatgggta
    14761 ataggatgta tttcttgggg agctttaata gtaggttttc ctacatcatt atcagcagta
    14821 tgggctccag caggtgaacc acatatacaa aaaattataa ttgtagctat gatagttggg
    14881 cttacaatta taaacttatt aggtgtatct ctatcaaaaa ttgtacaaaa tgttataact
    14941 gtaggtaaac taattccatt aatactattt ataggaatag gtatattttt tataaaagga
    15001 gttaatttta cttcttcaac aatggtacct ccaggagctg gagcaacaga gtttggagca
    15061 gcagcacttt taatgtttta ttcatttaca ggatttgaat ctatagcagt tgcagcagaa
    15121 gatatggaaa atccacaaaa aaatatacca atagcaatta taagtgtaat agtaattgca
    15181 tcaataatat atatattaaa tcaagttgta tgtgttggta tactaggaga ttcgttatct
    15241 agtacatcta caccagtggc agatgctgca agaatatgct ttggaaatat gggagctgga
    15301 ttggttacct ttggaacatt ggtttctgtt ggtggaattt gtatgtgtgg tgcttttgta
    15361 aatccaagaa gttgtgtagc tttagctgat gataaaatgt taccgagaat atttgcaaga
    15421 aaagataaaa aaggtacacc atatgtagcg ataatagcta ctatgttaat cactatacca
    15481 atagctctat ctggaagttt tgctgaatta gcagcaataa gtgcagttgc tagattcata
    15541 caatacatac caacatcttt atccgtatta gtgtttagaa agaaaagacc agaattagta
    15601 ggtactttta aaactccttt tggagcagtg attcctctaa ttgctgtatg tgttggaaca
    15661 tggttgttat tccaagcatc tatgcatcaa ttaattatgg gattaggagc tttggcaatt
    15721 ggagtaccat tatactttat aatgaagtct tataatagaa aaatctatgg tgaaaattta
    15781 aagaaaatag tttaattatg ttataacaat ttattgagtt aaaattagac attggcagaa
    15841 aattaaatac aaataagtat ctacaaggct atattaaaat attattaatt ttttatttta
    15901 atatagcctt atattgttat caataaatga aaaatattgt aacaacagaa gaacaatagt
    15961 attatgttta tagacaaaaa acttattagg aggtaaattt atgccaacaa taacattaaa
    16021 aaatggtgta gacgtactta ttagagaagg tgtaagagaa gatgctcaaa gcattataga
    16081 tttttataat gaagtgggtg gagaaactca ttttctgtca tttggaaagg atgaatataa
    16141 aatatctttg gaggaacaag aaaatgctat agaatcagca aaagcatctg ataattctgt
    16201 taaactgatt gcatttatag atggtgaaat agttgggatt gcaacgattg attctaatca
    16261 aaaagcaaaa ggaaagcatg ttggagtatt aggaattgtt gtaaaagaaa aatattgggg
    16321 aattggattg ggtaaaaggc ttatgttaga ccttatagaa tggtgtaaat caaatggaat
    16381 aaccaagaag ataactcttg tcactaatga agaaaattat aatgctatag gattatacaa
    16441 aaaagttgga tttgaagtag aaagcatact aaagaaagaa tgttattata atggtgttta
    16501 tactgattta ataggaatgt cacttttatt gggaatataa ttagtgtaca ttttatctcg
    16561 aaaaaagtat attaagtatg gtataattaa aaaagaatat aacttaatat aattttggag
    16621 gacgaattat gaaaaatctg tgtaaaatat cagatgatta tttaaacagc aagcttaagt
    16681 atttaaagtt attatcaaaa caatatccaa gtatatcaaa agcaagtaca gaaataataa
    16741 atttagaggc tatattaaat cttccaaagg gaacagagca ttttataaca gatgtacatg
    16801 gtgaatatga accatttgta catgtattaa aaaatggttc tggtgtaata aagagaaaaa
    16861 tagaagaatt gttttcaaat acaataagag acagcgaaaa aaaaatgtta gcaactcttg
    16921 tatattatcc tgaacaaaag ttagacctaa taattaagca agaggaaaat atagatgatt
    16981 tttatagaat aaatatttat agacttatag aactttgtaa atatgcttct agcaaatata
    17041 ctaggtctaa agttagaaaa ctgttaccag aaaattttaa atacataata gaagaacttt
    17101 tgcatgagca tgttaaaagt gaacataaag aagagtatta taaaagtata gttgaaacaa
    17161 tagttgacat aggaatagct aaggagttta taattgctat ttctacagtt atacaaaaat
    17221 tggttgtaga tagacttcat gtaataggag atatttatga tagaggtcca agaccagata
    17281 taattgtcga taagcttatt gaacatcatt gtgtagatat tcaatgggga aatcatgata
    17341 tattgtggat gggtgcagcc tcaggtgaaa aaacttgtat agcaaatgca ctgaggatat
    17401 cagctagata tgcgaattta gacattgtag aagacatata tggaataaat ctattgcctt
    17461 tagccacttt tgctatagaa atgtataaag atgacccttg taaagaattt attccaaagg
    17521 ttaatgacca aagtgtaaca acaacagaaa agtctttaat ggcaaaaatg cacaaagcaa
    17581 taagtataat tcaatttaag cttgaaggtg aggttatcag acgaagacct gaatttgaaa
    17641 tggaacatag actcttgctt aatatgataa actatgatga aggtacgatt aatttaaaag
    17701 gtaaaacata taaattaaaa gatacttatt tacctacaat agacaaaaaa gacccatata
    17761 aattaactat ggaagaaaga aatgttattg ataaattagt atcttcattt agaggtagtg
    17821 aaaaactaca aaaacatgtt tcatttttgt tttctaaagg aagtatatat ttaaaggcta
    17881 actcaaattt actgattcat ggatgtgtac cgttaaatga agatggaagc tttatgtcta
    17941 tgaatataat gggaaaagag tataaaggta aggctcttat ggataagatg gagtctctag
    18001 caagagaagg atttttcttt aaagataagg cagaagaaaa actgtatggc atggatatta
    18061 tgtggtattt atggactggg aaatgttcat cattatttgg taaagatgat atgactacat
    18121 ttgaaagata ctttatagct gaaaaagaga ctcataaaga aaataagaac ccatatttta
    18181 aacttagaga aaatgagatg gcttgtaaaa gattatttga agaatttgat ttagaacttg
    18241 atgaatctca tataataaat ggtcatgttc ctgtggagag taaaaatgga gagagcccaa
    18301 taaaagctaa tggtaaaatt ctcgttatag atggtggttt ttctagggct taccaaaaaa
    18361 ctactggtat agctggatac actttgatat acaattctag aactttgcaa ttagtatcgc
    18421 atgaaccatt taattctgct gaagaagcca ttgcaaatga aagtgatatc ttatcgacaa
    18481 ctgttgtagt agagcataag gcaaagagaa aaatggtgag agatactgat gaaggaataa
    18541 agatacaaga ggaaatagaa gatttaaaac tacttttaat ggcttataag aaagggctaa
    18601 taaaagaaat gtagatgtta tagaggcact tcatgattga agtgtctttt tgttaatact
    18661 tattaaatat attataaaga ttaataatag tagtttatat tatacttact aagtgaatcc
    18721 aagataaatg ggttcacttt ttttatttat aaacattatt aaaaaaatat agatgttttg
    18781 gttatattcc ttgtccttta taaatagaat tatataaaca aaggaggagg acaagagatg
    18841 aataaacttt ctgatgaaat aataaactct ctttctacaa ctataagaga aaaggtagaa
    18901 aaagtctcaa acaataatct gaacatagaa gaaattaggt tacgctcaca aaaaccattg
    18961 atactaaatg caaactctaa agattatttt tacaatcaaa aaactatgac acttgattta
    19021 aatcaacaaa actcatatgt agtaacaaga gaagatgtag agcaaacttt tcaaataata
    19081 tgtaaatatt caatacattc atttatggat gacataaaaa aaggattcat aactttgaga
    19141 ggaggtcata gagtagggtt agttggaaaa gcaatagtag aagatggaca agtcaaaaat
    19201 ataaagcata tatcatcttt aaatataagg gtatcaaggg aaattattgg atgctcggat
    19261 aagattttaa gtcatatcat aaagggaaaa aatcagatta ataatacttt gataatatca
    19321 ccacctcaat gtggaaaaac aactttaata agagatatag taagaaattt aagtaatggt
    19381 aatgaagatt atggatttaa aggtttaaaa gtggctttag tagatgagcg taatgagata
    19441 gcaggagctt atctaggtgt tccacaaatg gatgtgggaa taaggactga tataatagaa
    19501 acttgcccaa aagacttggg tattacaatg cttttaaggt ctatgtcacc aaatgttatt
    19561 gtgacagatg aaattggaag tgaaaaagaa ataaaggcat tatacacagc attaaatgga
    19621 ggtataggat taataactac tgtgcatgga gattcaatag aggatataca aaatagaaaa
    19681 gaattaaata gacttctgga taaagagtta tttaaaaagg ttataattct ttctgcaaaa
    19741 agaggagcag gaacaattga aaaaatttat gatttagaag aaaagagatg gtattttgca
    19801 aattaaaata attattatag ctttcttgat aggaagtagt tatttgatag gtgaacaaat
    19861 atacaaaact tatactcgta gacataaaca attaaacgat ttaataagag ttttagaaat
    19921 tttgaggatg gatttatctt ttggacttta tacattagaa gaaatcttta ataggatagg
    19981 tggaaataaa gagttttgtt tttggaaatt tttctatcaa atatcagaag gacttcacaa
    20041 tgaacaaagt aaaactttgg aaataataat atcagaaaat attgatgtct taagtaaaga
    20101 aacttatcta ggcaataaag aaattgagga actgaaaaat ctaatactta ctttagggaa
    20161 aagcgatata gagtcacaac aaagaatgat agatttatct atagaaaatt tgaaaaaaca
    20221 aacctatgag acaaaggaag atataaacaa aaaaggcgta ttgtataaaa aattagtgac
    20281 attcataggt ataggtatat gtattatatt gatttaatat ggggaggttt attatggaaa
    20341 tatcattgat attaaaggtt gcaggtgtcg gtattctaat atcagtattg aatatgatat
    20401 tggaaaaaac agatagaaaa gattgggcag gtcttactac attagcaggt gtaataatcg
    20461 tattgggtat ggtaataaca gaaataagtg acttgtttaa tactgtaaga acaatgtttc
    20521 aactttacta aagaggaggg agtaatttgg aaataatgca attaatagga attgcaatca
    20581 tttcaactac actctgttta gtaattaaaa aagatagacc agagatagca aatttcatag
    20641 ctataataac tggagtaata atactattgt cagttatgtt taagctaaat tttatagtag
    20701 atagtataca agatttggct aacaaggcta acatcccaac tatgtatata tcattaataa
    20761 taaaattaat tgggatagca tacttaatgg aatttgcgat acaactttgt aaagattgtg
    20821 gggaaggaaa cattgcttca aaactagaat ttggaggtaa aataatagtt atgtcaatgt
    20881 ctttccctat acttttgtcc attgtagaaa tggtggtgaa tataattcca tagatgtgta
    20941 tcttggaatg aatataagta tattagagag ctttcgttgt attgaggttt atagagtgaa
    21001 gagaggcatg gttgcttatg aaaaaaagat ttttaccaat gattataggt tttttattta
    21061 ctttcttttt tgtaaatatc tttgctatta tgatatttgc taatgaagta ccaagtagtg
    21121 aagataaaga atatggtgaa acaaaaaata gcatagataa gtatatagat ggtcaattag
    21181 acaagctaga tataaatgag attcaagact atataaataa agaaatagtt attaatgatg
    21241 ttaatttaaa atcatttgta aaagatttaa ttagtggaga aaaaaatatc ttagatttat
    21301 tcaataaaga tggattgaag atattgatgt ttgatgaatt taaagcaagt ttaaaggtag
    21361 tagcagtaat tttggtactg gctctgttat catctatttt aaagagtctg gaaaactctt
    21421 tttcatcagg tgcagtaagt caaatagcaa catatataat atttatcaca atggtatcac
    21481 ttacacttat tggttttaaa gatgtattac agatttgcta tgatgcaata gaccatactg
    21541 taggtcttat gcaagttata atgccaatat taataacatt tttactttta attggattcc
    21601 caataacgtc gactactttg aatcctatct tcataggagg agtaacattt ataaatgtat
    21661 tttttaaaaa ttttctattt gtatctatta ctgttgcatt tggaatttta ataataaaca
    21721 atttatcaaa aaatataagg ctaaagagat ttttctcctt tgtaaaacaa ataaattatg
    21781 tttctattgg agctatgttt actgtttact taggtcttgt atccatacaa ggattgtatg
    21841 taaccagttt tgataaattc agtgtaaaga ctgctaaatt tgctataggt aattttattc
    21901 ctgttgtagg tggatttgta tctgactctg tggatatttt attatcttca tctcagctta
    21961 taaaaaatat atttggagga ataggactta tcttacttgt tggaatttgc ttacttccag
    22021 taataaaaat tttatcagta atagtagtgt acaaattagc agcaataatt gtagagcctg
    22081 ttggagagga tggaatatct aattttttaa atgaagtggc aaatttaatg ataataatgc
    22141 tagcatcagt aattgctata acagtaatgt tctttgtaac tgtagctatc ttgacctcga
    22201 taagtgtcgt aagtcaaggg taatttatat atcagtgaaa aataattaca tttaatctaa
    22261 tgtttttaga gaggaggatg ctatgttaga gggcataaag gcatggatag ttagtgtttt
    22321 gataggagca tttatagtaa atatagtaga tatgatttta ccaagttcta agataaaacc
    22381 ttatgtcaac ctagttttaa actttatgtt tgtatttatt gtaataactc cagtagtagg
    22441 atttttttca aaagatatga gtttagaaga tagaatatta aaatctatgg gaaactacaa
    22501 caagcagtat gtagatagta caaatgcttt ggcaaaagag actggaaata atagtctatc
    22561 caagggatat gaggatgggt taaaagaagt acttaaatta aaacttgatg aatatggtta
    22621 tgatttagaa gatatagagc ttaatggggc aaatataaat aatataaaaa taaaagaaaa
    22681 gaataatagt gcaaaaagta ataacagttc caatgaagaa aataaaaata atagtactaa
    22741 aagagaggaa gaaaacataa actctaatga caaagaaaat tctaaacaag tcttcaaaaa
    22801 aggtacagag tatgggttga acctaaatga agaaaaatta aaaaacgact taataaaggt
    22861 gctggatgtt tctatagaag atatacaaat tgataaatag gagttgggag aatgttgaaa
    22921 aacttaaatg acaaagataa gagaaaggta tattcactaa taactatagc aggaatatgt
    22981 gtggtttcat tagtattatt atcttgtttt cctagtagca aaagcgataa acaagttgga
    23041 aaaacagaga ctaataaaaa tgctgaaaag caagtcacta aagaacaaga aaaagatgat
    23101 ttagagtcaa aattaacagc aatactatct aaaattgatg gtgcaggaga cgttgatgta
    23161 atggttacat ttgaatcaag tgaagaaata caacctgctt ttaattcaaa taatacaaca
    23221 gaaacaacag aggaaaaaga tgcacaaggt ggagaaagaa cagttacgac ttctagtgaa
    23281 aataaaacaa tgataacttc aaattcaagt gaccctgtag ttataaaaac tacagagcca
    23341 aagataaagg gagtgatagt ggtagcaagt ggagcaagtg acccaagtgt taaagagaca
    23401 ctttatagtg cagtacaaac atcattacaa gtagctggtc atcaagtaga aatatattct
    23461 aaataaatac atataaccgg ggggataaat gaatgaagtt taattataag ggaagaggat
    23521 ttgtaataat aactttaact gcaatgttag tggtagtagg aacagtgaat tatcaactaa
    23581 gtaagaaatc tttattagaa acatcaaaag agtttaaggc atatgaagaa gcacagttgc
    23641 aaaaaaatac tgatgagagt gatagtagta agacagagga ttctagtaat gtagataaac
    23701 aagatggtaa ggaaagtgct gatattgata tagttgatag caaagctagt aaagtaaaag
    23761 aaaaggcaac tgagacaagt aaggaaataa aagctcaatt atcatcagag aaaaatatga
    23821 aaaaggcatc ttatatttta gatatgaaaa tgaatagaga aaaacaaaga aatgagttag
    23881 ttcaagattt aaatgaaatg ataaataatc catctacaac agaagaatct agaaaagaag
    23941 cttctaatat gaaattaaat atagttaaaa ttcaagaaaa agaacttcaa atagaaaacc
    24001 ttttgagtac aaaagggtat gaagaagcgt tagtttatat aagcgataac aaagttaatg
    24061 tagttgtaaa tgaagctaaa ttagaaaaga aagatgcagc aaaaatattt gatttagttg
    24121 cagaacaagc caatgtgaag tatgaaaata ttaaacttac aaataataat agtaataagt
    24181 aaagtataaa cctcagaatt aactgaggtt attttcttgc aaagtagact tgtatttggt
    24241 attattatac ttataaaaat agttccttaa aatcaggagg taagttttaa tatggaagat
    24301 aataaatttg gacaagtaaa aatatctaat gatgtaatag ctacaatagc tggtttagca
    24361 gcattagaag ttgaaggcat agaaactaca gcaacattaa cagataagtt gttaaaaaat
    24421 aatggagtaa aaatacaaat cgaagaagag gatgttaatt tagatgtaat ggttacgata
    24481 aagtatggaa tgtcaatacc agatacagct tttaaagttc aagaaaatgt aaaaaatact
    24541 gttgagacaa tgacaggact gaaggtttct caagtaaata tacacattca aggaatcagc
    24601 tttaagaagg ataaagttga taaagaagaa gcaaaagcaa ctaagaaaaa ttaatattag
    24661 ccctctgaaa agtcagaggg ttttttgctg gtaattgatt ttatatggat aattaaactt
    24721 aaaggagaat tttgattgat gaaaaaagat agggcacaaa agagtacaac aagagaatat
    24781 ataatgaaac ttatatacca aataaatatt aataaagaag attttgaaac tttagaagat
    24841 aaagtagata attttttaaa agataattca gaacatatta taaatagata taaagaactt
    24901 gcacttcaat actcaaaaaa tacaaattta aagttagaag atactgaaat tgaagatgtt
    24961 atagacaaaa aatatataaa tacagtatgc aaagcattga aagaaaatca tgacaaaata
    25021 gatgagttaa taaataagca tgctaaaaac tggacagtag atagaatgcc aaaggtagat
    25081 gtttctatac ttagattgtc tgtgtgtgaa atactttatt tagacactcc taataaagta
    25141 tcaataaatg aagcagtaga acttgcaaaa atatattgtg atgataagtc tcctaaattt
    25201 ataaatggta tattgggaag tgttgttgat gaaattggga aataatataa taattggaat
    25261 tgatactagc tgctatacta cttctatagc agctatctct ttagataaga aagttatatt
    25321 caatgaaaaa ataatgttag aagtaaggga taattcaaaa ggtttaaggc aaagtgaagc
    25381 tgtatttcaa catatcaata atttagggat tcttagtgat aggataaaat cttttaaaga
    25441 taagtttaat gtagaaggag tttgctcatc aaaaaaaccg agacctgttg aaaactcata
    25501 tatgcctgta ttcaatgtag gtcataattt tggaaaatta ttgtctagta tatatggttg
    25561 taggttttat gagactactc atcaagagaa tcatattgaa gcaagtttat taaatagtaa
    25621 attaaaaaat aataataaat ttatttctgt tcatatgtct ggtggtacta cagagatact
    25681 tttaacaagc aaacaagata gtcatcacaa tgtatgtgat actaatttag gtaaaattgc
    25741 caaaataagt attaaaaaag atgataaaag taagttatac aataattttg gatataatat
    25801 agatataatt ggaggaagta aagatattag ctttggtcaa cttatagata gagtaggaat
    25861 taagcttgga tataaatttc cttcaggaaa gtatttggat gaaaatgctt taaattgtaa
    25921 tcttaaaata gaaagtggtc tgaaaacttc tgtaagagat gggtatatga atttatcagg
    25981 attagaaaat caggtaaata agattataaa tgataatgga gataatacta atcaaaaaga
    26041 atatatatct aaattggtat tagattcagt agttagaaat atgtttaaat ctttagtgta
    26101 tttatgtgaa acttataatg taaatgaagt agtatttgct ggtggagttt ctgcaagtaa
    26161 gtatattctt agagaattaa gcatgaagtt aagaaaaaag catatagaag cttattttac
    26221 agaaccacaa tattcaactg ataatgctgt cgggtgtgct ataataggat tgaataactt
    26281 tttaggagaa agagtatgaa actaagagct ttagatataa gtgaagcaaa ctcatatatt
    26341 aaaagaattt taataaacga cccaatactt tctaatctta aagttaaagg agaaatttct
    26401 aattttaagg ttcatagtag tgggaatgta tatttatcac ttaaagatga aacatcaaag
    26461 ttaaactgtg taatttttaa aagtaatttt aatcgcaatt taaaattaga taatggtgta
    26521 aaaattatag ctaatggata tatatcagtc tatgaaagag atggtgcgta tcaattatac
    26581 attaatgaaa ttgaaataga gggaatagga aatttacaca tagagtttaa tagattaaag
    26641 gaaaaactta ataaagaagg attatttgac cctaaataca agatacctat acctaaaatg
    26701 ccaaactcaa ttggcgttat aacatctcca acaggtgctg ttataagaga tataataaat
    26761 gtaataaaaa gaagatatcc gaaagttaat ataaagcttt atccagtaac ggttcaagga
    26821 gataaatcag ctgaagaaat ctgtgaagca attagatttt tcaatcatat gaagaatgtt
    26881 gatacactta tagttggtag aggtgggggt tcgatagaag aactttggtc ttttaatgat
    26941 gaaatggtag caagagaggt ttttaattct caaattccga taatatcagc agttgggcat
    27001 gaaacggatt ttactatttg tgactttgtg tctgatatga gagccccaac gccatcagca
    27061 gcagctgaga ttgctactcc atcattggat gatataaact ataaacttgg aaacataaaa
    27121 agtaggatga gtaagtctct tacaaatcaa atagagctag accaatatag gttagaaaca
    27181 gtatttaata agataaataa ctatttagat tcgtacacaa taaaagataa agttatacaa
    27241 ttagacaaaa tatatgataa aataattttt ggaatagaaa ataatttaaa attagaagat
    27301 gaaaaattag taaaaattgg agcattactc cataatttaa gtccattagc tactatggat
    27361 agaggttata gtataactca aaaaaatgga aaagtaataa atagtataaa aggattaaaa
    27421 ataaaggata gtatagatat agtattaaaa gatggaaatt tagaatgtat gatagataaa
    27481 attgaaaata aagagggata gtatgaattt aacctacgaa gaagcttata aaagattaga
    27541 aagtatttta agtgaattag aatctaaaaa tgctagcttg gatgaatctt taagcctata
    27601 tgaagaaggt ataagccttt ataaacattg taataagctt ttagatgatg ctaaattgaa
    27661 aataagtaaa tttaatcaat tgggaataga agaagacttt aaaatagagg aggaataaaa
    27721 ttggaattta aacaatgtct aaaagaaaaa gcaagttttg tagaaaaagt acttaaagaa
    27781 tatatgccta aagaagaagg gtatcaaaaa actgttattg aagctatgaa ctatagctta
    27841 agtgctggag gtaaaagatt aagaccaata cttacattgg aggcttgtaa aatagttgga
    27901 ggtaatgaag atgaagctat tccttttgct attgcaattg aaatgataca tacatattca
    27961 ctaatacatg atgatttgcc agctcttgac aatgatgatt taagaagggg aagaccaaca
    28021 aatcataaag tatatggaga agctatgggg attttagcag gagatgcttt gttaaactat
    28081 gcttttgaag taatgcttgc aggctcaatc aataaagaaa atccagaaaa gtatctaaaa
    28141 gctataaatg aaattgccaa aggtgctggt atatatggaa tgataggtgg tcaagtagta
    28201 gatgtagaaa gcgaaaataa acaaattgga aaagaaaaat tagattatat acatatgaat
    28261 aaaactgctg caatgatggt aggctgtatg agagcaggag ctactatagg tggagcaaat
    28321 tctgaacaaa tggaagaaat aactaaatat gcaaaaaata tcggattatc attccagata
    28381 gtagatgata tactagacat agtaggagat gaagctaagt taggtaaaaa ggttggaagt
    28441 gatatagaaa atcataagtc aacataccca tctcttttag gtttagataa atctaaagaa
    28501 attgcacata atttgattga tgaagcaaag aaaagtatag aaaaattatc tgatgatgta
    28561 gattttttaa agggattggc tgaatatata atagatagag aatactaaaa tatacagcta
    28621 aaatcgagga gggaaaaatg gatttttttt cggagatttt taacaatggg gctttgggaa
    28681 taagtctgat tgcttgtttc ttagcacaat ttataaaaat atttactggt aaggaaaaaa
    28741 gaattgagtt atcaagaata cttatttcag gaggtatgcc tagttcccat agttcttttg
    28801 ttacaagttt agctacagtg gtaggtattg aaaaagggtt taattctact gattttgcta
    28861 taattaccgt gcttgcctta attataatgt atgatgcagc aggtgttaga cgggctgttg
    28921 gtaaacaagc tactatatta aatcaaatgg tggcagatat acagcatgga aagcatatag
    28981 agcagaagaa attaaaagaa ttaataggtc atacaccact agaagtttgg tttggagctt
    29041 tacttggaat agtaacagct ttaatattga tgtaaattga aaatttacag aataaaggtg
    29101 aggataatat gtataaatat ttagataaag taaattctcc aaaggatata aaaaatatga
    29161 gtatagaaga aatggattta cttgcaaagg atataagaaa gttcttagta aaatctgttt
    29221 caaagacagg aggacactta gcttctaatc taggagtggt tgagttaaca ttagcacttc
    29281 ataaagtatt tgatagccct aaagataaga ttgtatggga tgtagggcat caatcttatg
    29341 ttcataaaat agttactgga agaaaagatt gttttgtatc tttgagacag tttaatggac
    29401 taagtggttt tccaaaggag aatgaaagtc ctcatgatat ttttgataca ggacatagca
    29461 gtacatctat ttctatagct acaggtattg cctgtgctag ggatataaaa aaagaaaact
    29521 atagtgttat atctgtgata ggagatggtt ctataacagg aggtatggca cttgaagctt
    29581 taaatcaatt aggatatata gataccaata tgatagtaat ccttaatgat aatgagatgt
    29641 ccatagataa aaatgttgga ggaatgtcta aatatctgtc aagtattata agaaactcaa
    29701 cagttgagaa aatgacagat gaagttgata aaattttaaa tgttacacaa acaggtgaaa
    29761 ttttatctaa gactgcacat agatttaaag ataaactaat gtatagcttt tctcctcagg
    29821 actgttcatt ttttgattca ttaggtataa gatattatgg acctatagat ggccataata
    29881 ctaaagaact aatagatata ttgagaaaag ctaaacacaa aaaagggcct gttcttttac
    29941 atgtaattac gaaaaaagga aaagggtaca gatttgcaga agaacaacct gataaatatc
    30001 atggagtatc aaagtttgat ataaagacag gtgttacatc tgcaaaagtt aagtctatgt
    30061 caattagtgt tggagaaaaa cttgttgata tggctaatag taatgaaaat atagtagcaa
    30121 taacagctgc aatgccatct ggtacagggt taaacctgtt tgaaagtgca tatccaaaaa
    30181 gatattatga tgttggtata gctgaacaac atgcaacagg atttgcagca ggtcttgcaa
    30241 aaaatggtat gaaaccttac tttgctgtgt actcatcatt tttgcaaagg gcatatgacc
    30301 aagttattca tgatgtatgc ataacaaaaa agccagttac ttttcttata gatagagctg
    30361 gattagttgg aaatgatgga gaaactcatc atggtatgtt tgatttaagt tacttaaatt
    30421 ctattccaaa tattgtagta atggctccaa aagacacaag agagatggag cttatgatgg
    30481 atttgtcatt aaaattagat tgtccactag caattagata cccaagagga agtagttatt
    30541 acctagataa gggagagtat ggagagattg tactgggtaa atatgaggta ttagatgacg
    30601 gtcaagatac agtcatactt tgtattggaa gtatggtaaa acatgcttta gaggctaaag
    30661 aaatattatc aagagaagga ataaatccga caatagttaa tgctagattc ttaaagccaa
    30721 ttgatgaagg tatgttaaaa gcattattaa aaaatcataa aaatgtagtt actatagagg
    30781 ataatattgt aactggagga tttggaagta gaataaataa gtttattata gataatgaat
    30841 ataacgtaaa tatacttaat atagcaatac cagaagaatt tgtaaaacat ggcaatatag
    30901 atgaattata tgattttgta ggattatcgc caaaaagtat tgctgataaa ataaggaaat
    30961 tagttattga gtaaaaatct aatgtagata ttgtaaatta aaatttatat aaaatataga
    31021 atttatcaat tagaataaat atcaagtgtg ttacttcaag ctactttagt tatcattaaa
    31081 gtagctttta acagaaaggt aattgtatga aaaaaagaat agatttatta ttagtagaac
    31141 aagggtactt tgaaagtaga gaaagagcca aaaaagcaat aatggctgga ttggtttttg
    31201 ttgataatca aaggtgtgat aaggctggta cagaagtaaa agaagattgt agtatagaag
    31261 taaaaggaaa tccaatacca tatgtcagta gaggtggttt aaagcttgaa aaagcaatga
    31321 aaaactttga tttgactatt gatggaaagg tatgtatgga tataggggca tccacaggtg
    31381 gttttacaga ttgtatgctc aaaaatggag caataaaagt attttctata gatgttggtt
    31441 atggtcaact tgcatggaaa ttgagacaag atgatagggt tgtgtgtatg gaaagaacca
    31501 atataagaaa tgtgacaata gaagatacaa agcagtttgc tgattttgcg tcaattgatg
    31561 tgtcttttat atcgttaaag ttagtacttc caaaggcaaa ggagttagta cgtcatgatg
    31621 gggaagttgt cgctttaatt aaacctcaat ttgaagcagg aagagaaaaa gtaggtaaaa
    31681 aaggagtagt tagagaaaaa tctacacata tagaagttat aaaaatgatt tctgatttct
    31741 cagtcgaaaa tggatttgag attttagggt tagatttttc tcctattaaa ggtcctgaag
    31801 gtaatataga atatctaatt catttgagaa atggaaatga aggatatgaa tttgatggtg
    31861 agacatacaa taacaaaata gtagaagttg ttgaagcttc tcataattta gacaaatagg
    31921 ttaaaagggg gcttagattt gatcctcgaa ttatatatga aaaattgtgc cttagttgaa
    31981 gaactaaggt tgaatattga taaaaactta aatatactta caggtgaaac tggttcagga
    32041 aaatctataa taatagatgc acttggactt tgtttaggtg ataaatatga taggtctttt
    32101 ttaagaaaag gaacagataa gggacttgtt gaggctgtat ttttttcaga caatagatat
    32161 cttaaaaaaa tattagaaga aaatgatata agtatggaag atgataattt attggtaata
    32221 actaggttga tatactcaga tggtaagagt actgctagag taaatggaag gactgttaaa
    32281 gtgtctcttc ttaaagaaat tgcatctaca cttatagata tacatggtca acatcaaaat
    32341 caagctttgt ttaataaaga tactcattta aaatttctag acctttttgg agaaaatgaa
    32401 ttggaagagt ttaaaattgc ttataaaaat gtgtaccata agtatagtga agtaaaaaaa
    32461 gctttaaatt gcttgacaga aaataaagat gagatgcaaa ttcaacgaga aatagattta
    32521 ttaagatttc aaataaatga aattgaagct gcaaatctaa ataagaatga atatgaagat
    32581 ttattaaaac aaagagaagt ttatagaaat agtgaaaaga tatataataa tctaaattcg
    32641 agttatagca agttacataa tggtgagtat aatgttattg acctaatagg tcttgcatct
    32701 aaggaactta atgacatatc taaatatgac agtgttttaa gtgaatatag tgatactgta
    32761 gaaagaatca tgtatgagtt acaagacata tctggtgaga taagaaatta caaagataat
    32821 atagactttg aaccttatga gctagagcag attgagctta gaatcgacga gattaataac
    32881 ttaagaagaa aatatggaga cagtatagat gatatatttg agtattatgg aaaaattaaa
    32941 gatagattag atgaaatatt aaacagagat gaaagagttg aacagttaag aagcaaactg
    33001 atgaatatag aagaagattt aaaaataaaa gccagtaaat tgactaaagc aagaatagaa
    33061 gtagcaacta ctttagaaaa aatacttctt gatgaattaa aaagcttaaa tatgaaaaat
    33121 gtaatgttca aggttaattt tgaaaaaggt ccatttactt tagatggagt agatgatatt
    33181 gaatttatga tatcttttaa cttaggagaa gatattaagc ctatatataa agttgcatca
    33241 ggtggtgaga tgtccagatt tatgttagcg tttaaaacca tattggctga tatagatgat
    33301 attgatacat tagtgtttga cgagatagat actggaataa gtgggatagc agcacagata
    33361 gttggtgaga aattgagtga tatagcaaag aaaaaacaga taatatgtat aactcattta
    33421 cctcaaattg ctgcaaatgc agacactcat tattgtatag agaaagatac ttcaaataat
    33481 aggacgttta ccaatgttag taagttaaat gaatctcaac gaaaaaatga aattgccaga
    33541 cttatagcag gtaacaatat aacagaaaaa actattgaac atgcaagtga aattattgag
    33601 ttggctaaaa aatgttagtg aaaaacaagc attttaatag gtgtaaagaa tttttataga
    33661 aatgctttac atctattttt tatgatgaaa ttattttaaa atatctagtg cctatattga
    33721 agaaaagtag atttaaaaat agaagaaaat ttgtataaat ttgtaacttt gaaatttcta
    33781 aatgcttaag tataatatta tttattaagg tagtagtgag ggggacataa aatgaataaa
    33841 aaagatacta tgcacttaat ttttacaatt gtaattttta ttttattatg gtatattgtg
    33901 gtagcagtaa gtgagattaa taaaaatatt gaaagttgta gtaataaatt agattatttg
    33961 tcacaagaga ttatgagtac taaatcggat attagtaata ctataaatga agaaatgtca
    34021 aaaagttata taacaaagag tattgactta aaagtaaaaa aaatcgagaa aaaagagtgt
    34081 gttattgatg tagatgttca attaagtaaa cttggaaaaa atggaaaagt attttttatg
    34141 tataaagaag atagtgataa atggaatgaa actgagatga caaaacatgg agaattatca
    34201 tatgcatgtg aaattaagat aaatacagga agtgaatatg actataaagt agttacaagt
    34261 ggagctatat ctgaaagttc tgatgtgcaa aaattgacta aatctgatta tatgcctgaa
    34321 caacccatat ttaattctgg gataagagat aatagagaat actttattga aatagtagaa
    34381 aatagtaatc ttttcaatca tgagagtgaa aagtcaaaaa ataaagttta tgataatatt
    34441 agattagaaa aagtagatat tattgtaaat gaaggaaaaa aagacactat atacaaagcc
    34501 aaattagctg aaggcttaga tgatggcaaa actaataatt caaatagaaa tcaagtgaat
    34561 tatgaagtaa gttttcctaa aagggatatt gatgacataa tttatattaa agctaaactt
    34621 acatacaata atggagtaga atatacaaag gatataactg aagatataac tatgggatta
    34681 caagatttag aaaaataaat acatatttaa ataaatattc aaatagattt gttcaataaa
    34741 atagagtata aataaaactg tagtagtttt acttatactc tattaaaaaa ttataatttt
    34801 aattccatat atctagcccc ttcaattgga ttataagtat aaggttctat atcataaaaa
    34861 ccaaactttt catataatcc ttgtgcactt ttcatgcttg gaagagtatc taatctcatg
    34921 tgtgtatatc caaatttttt agcttcttca atgatttctt ccaatagtat ttttcctatt
    34981 tttaatcctc taaattgatt tcttacatat aatcttttta attcacatat tttaccttca
    35041 agttttttta aagcaacaca acctgccaaa ttttcatcaa caaatgccaa tatcaatgag
    35101 cctgaaggtt ttttatattt tcctggaagt gtttttaatt cattattaaa gtcttgaaaa
    35161 cataagtcta tatttagaga atttgaatat tcagtaaata gaagttttac gttttcaagg
    35221 ttttctaaat ctttttcttc aacaaattgt aaagttatat tttgagccat tttatccgcc
    35281 attatataaa catgtttata taatgacttc acctccttga tatatgtata tttttcttaa
    35341 tatcaattag attttaatac cattactttt tcaccaccat agtcaagctc tccattaaac
    35401 tcaaatccaa aatctttata aagcttaata gccattttat tgtcttcgaa tatactaaga
    35461 tatagttcat cacagttata ttcattttta agatgattta caagaaaatc taatgatgct
    35521 tttccatatc ctttaccttg atattttgaa tcaatcataa atctatcaag ccaaactcta
    35581 tcatcttcta aacgaccata catagcaaat cctattaaag aattttcatc atatattcca
    35641 acaggtaccc aagctgttat aaatttagat tcagcaatag aaagtgcatt gctttctata
    35701 taatctaact gacttttatc tacagacaaa ctaactaaat catgccaatt atttatgtct
    35761 gcaattctta catttaattt caagatatta ccgcccttca aactttttgt tacaatctaa
    35821 tactatcata ttatgtatta aaaatcactt attaagattt taaataaact ttttaaatga
    35881 ataaattctc aggaaaaagg aattataaat acataataat acagcatagg agtagatgga
    35941 tatatgaaca aaataaataa aaatttcaaa aatatttgga taatattagg agctgtatcg
    36001 atttttacca tggttataat ttttaataaa aattgtaatg ttaataaaga gactcaaata
    36061 gaagctttta caattaagaa tcatgataaa aaatttgtat atcctatggg aaatataatt
    36121 ggagtaaaag caaatacaga tggtgtattg gtcttaggat atgaggaaga ggatgtagac
    36181 tatattggag gcatacaaat cggtgataat atagtaaaaa tagataataa aaggataaaa
    36241 aatagtcaag atgtatcaga gattttgaat aagattaaaa aaagtaaagt tgaagtgact
    36301 tttgagagaa aaaataagta taaaacagaa gtaatagaga ctaaaaaaga aaatggcaag
    36361 tataaacttg gattatgggt aagagataaa atatctggaa ttggaactat gactttttac
    36421 gaccctagta tggaaaagtt caaagcaatc ggtcatgcca taaaagattc ggatactaat
    36481 gaactattaa agataaagca aggatatata tataagccag aacaattaaa aatagtaaaa
    36541 gctagtaatg aaaaagtagg taaaataaaa ggagatttta atgacagcaa tttaatgggt
    36601 aatttctcaa ataattcaga gttaggtata agtggtaata ttacagaaaa ccataataaa
    36661 gagtttaatg tagcaaataa agaaaagcaa ttaatagaag ttggaagacc gcaagatgta
    36721 aaaataggag atgcagtaat tctttttgag gataaaaaca aaaatataac aagctatgat
    36781 ataaaaatag aaagtatagt atatgataaa ggaaattata gagatatggt aataaaagta
    36841 gtagatgaca agttattgga atacacagga ggtatcgtac aggggatgag tggagctcca
    36901 ataatacaaa ataataaaat tattggtgca ataactcatg tttttagaga taatccgaaa
    36961 aaaggatatg gtatttttat agatgaaatg ataaaattgt aggtgaggca ttaaaaaatt
    37021 ttattatttt atcaattatc taggaggaat ataattttgg agtgtcgaat atgctttaga
    37081 gtagataatt aggaagcaat tgtgtaaaaa gtttagtttt ctgtaataag aagatgtttt
    37141 ttaatggggg gatttttagt ggaaaaaatc aaaatagttt tagcagatga caataaggat
    37201 ttttgtcagg tattaaaaga gtatttgtct aatgaagatg atatcgatat attaggcata
    37261 gctaaggatg gaattgaagc attagactta gtaaaaaaga cacaaccgga cttattaata
    37321 ctagatgtaa taatgccaca tctagatgga ttaggtgtaa ttgaaaaatt gaatactatg
    37381 gatataccta aaatgccaaa aataatagta ctatcagcag taggtcaaga taagataact
    37441 caaagcgcaa taaatctagg agcagattac tacatagtaa agccatttga ttttgttgtg
    37501 tttataaaca gaattagaga gctagtttct aatagagtta cacaagttga gccaaaacct
    37561 agaccagttc aagagactca aatgactaga agtgattttg ttaagaatgt aggaaatata
    37621 gagacagaaa tcacaaatat aatacatgaa ataggagtac cagctcatat aaaaggatat
    37681 ttatatttaa gagaagcaat aaaaatggtt attgataatg ttgagctttt aggtgcagta
    37741 acaaaagagt tatatccaag tatagctaaa aaattcaata caacaccaag tagagttgaa
    37801 agagcaataa gacatgcaat agaggttgca tggagtagag gaaaagttga cacaataaat
    37861 caattatttg gatatacggt acacaatact aaaggaaaac caactaattc agaatttata
    37921 gcaatgattg ctgataaatt aagattagaa catagtatgg ttaaataaac aagacataaa
    37981 aagtaaggct ttttaattaa ggcattggct ataaatgcgt attacaagca gcgaaacggt
    38041 ataaccacta gggttatacc gtttcgctat tttaataaat ataaaaattt tctttattat
    38101 ttgcttacta tatcaatata ataattttat tatactatgg atatagtatg tgtctttaca
    38161 agttgtaaac tgacagtggt ttatttttta atataaatat tgactttgat gcaggtaaac
    38221 tttgtatttt taagcgtatt atggaatatg ttaaataaaa aaatgatgaa atatagtatt
    38281 gtaaatgcca aagatgcaaa acaaacttaa aacatttatt ttattgttaa gtaatgctat
    38341 aatataatgt gattttaata atgatagtgg aggtttaaat atgagagtcg aggcccctat
    38401 aaaagtagat cgaaaaacca aaaaacttgc taaaagagtt gaaagtgggg aaatagcagt
    38461 tataaatcat atagacatag atgaagttgc tgcaaactct ttagtagaag ctaaaataaa
    38521 acttgtcata aatgcggctc cttctataag tggtaggtat cccaataaag gtccagggat
    38581 attaactgaa aataatatac taataattga taatgttgga gaagagttat ttgaagaatt
    38641 aaaagaagga gaaactatag aagttgtaga tggaaagata tatagaaaag gaaaattttt
    38701 aggtgcagga gaagttttag acaagtatga ggtttcacac caaataaagg ctgcctatga
    38761 gaatttagct gtagaattgg atagatttat agataatact atagattatg ctaaaaaaga
    38821 aaaaggattt atacttggag aagtagaaat tccaaaagtg aaaactaatt atgctaataa
    38881 acatgtattg atagttgtaa gaggtcagga ctacaaagaa gatttaagta ctatgctttc
    38941 ttatatagaa gaggtaaagc cagtattggt tggtgttgat ggaggagcag acgctttaat
    39001 tgagtttgga tatactccag atgtgattgt aggtgatatg gatagtgtaa gtgatgaagc
    39061 tttaaaaaag gctagtgaaa tagtagtaca tgcttataca gatggaagag caccaggtct
    39121 taagagggtt gaggaattag gcttagatgc agttgtattt cctgcgccag gaactagtga
    39181 agatatagct atgcttatag cctatgagta taaggctgag ttaatagtag cagttggtac
    39241 acattcaaat atgattgatt ttcttgaaaa aggaagaaaa ggtatggcaa gtactttttt
    39301 agttagacta aaaataggtt caaagcttat agatgctaaa ggcgttaatt tgttatacag
    39361 aagtaaatta aaaataaagt acatatgggc attaatagca acagcattat ttcctgtgtt
    39421 ggtagttgca tcattgtcac caggagttca acagttcata caactaatgc aattaaagtt
    39481 tagagtatta ttacagatgt aaattttagg aggataaata tgcatattaa tatgaaatat
    39541 tatatagtca ctataggggc tatatttata gctttgggta taggcatgtt agtaggattt
    39601 aatttaaata ataatcaaca attaagtgaa caacaagcaa atataataaa tgatttggat
    39661 gataagttta atatcttaaa agaaaaaaat gataaacttg acagtgactt agcaagtgta
    39721 aatagagatt atgaagaggc agttaatttt ataaataaaa atgtagataa gattttagct
    39781 ggttcgctga atggtaagag tataggaata atatctgcta atgaaaatga cgattataca
    39841 aaaaatatag aagacataat aaataaatct aatggaagca ttgcatttaa tataatatta
    39901 aaagaaaaca taacaaatcc agaaaagtta aaggaaatgt caactaaact aggtacagat
    39961 gtaaaaaatg caaatgatgc agttaattat ataattgaca ctcttaaaaa ggaagatgca
    40021 agtgacattt taacatatct tcaagaacta gatgtaataa agtttaattt tattggagat
    40081 acatatctaa aatatgattc cgttgtaata gctggtggaa atgatgcaaa agacagtaca
    40141 aaacagtttg aaaaaataga aaaatttgta gtttcaaaac ttaaatcaga gaataaatat
    40201 ttagtagaag ttcaaaatac aggagttaaa acttcttatg tagaactata ttctaaaaac
    40261 aaagttgcaa ctatagacaa tattgatgaa ggtataggaa gtatatctct agcgattctt
    40321 ctacaacaag gtaatatagt tggtaatttt ggtagattgg atactgcaac ttcattgttg
    40381 ccatctatta aatagaaata attttaagga gagaaaaaat ggattcttat ataagtataa
    40441 taataccagc ttataatgaa gaaagtagaa taaaatatac attagaaagc atattggaca
    40501 ttaagcaaat aaatgaaata atagttgtgg atgatggttc aattgacaat actgctaagg
    40561 ttgtatctga gctagaaaat gaaaagatta aattttttaa gttagataaa aatagaggta
    40621 aaggatatgc tttaaattat ggtatcaagt tagcaatgaa aaatgcagat attataggtt
    40681 ttctggatgg agatttagga agttctgcta aagaagttga aaaattaata gtacctatat
    40741 taaatggtga ggcggatgtt acaatagcca agtttcctcc tgcaaagaga aaaggaggtt
    40801 tgggatttgt aaaaggttta gctaaaaatt ctgtgtttga gatgactggt gttgagttag
    40861 atgcaacttt atcaggtcaa agaatattta aaagagaagt tttagaaaag tttgatgaaa
    40921 taccttttgg atatggagta gaagttggaa tgaccattga tatattaaag tatggattta
    40981 aaattaaaga agtattagta aatatgactc ataatgaaac aggaagaaat ttaaaaggtt
    41041 ttattcatag agggaaacag tattatcaca taaaaaaggt tttaggacaa aaaaagaaag
    41101 aatggaggta atatcttagt gaaagattat atactttata ccattttatt attaactgga
    41161 ttatttggaa cctatgctgt aattccacta tttaaaaatt tattgataaa tggaaatgta
    41221 cttagaccaa actataaaaa agatatgata ccagtaagta tgggaattgt atttttgcct
    41281 atgattgtaa tcaatggaat aatacttgct ttctttacta cagaatttaa gaatttagcg
    41341 tatatattta tgtttatgtt tggtatgata tccatgtttt ttgcagggat actagatgat
    41401 gtcattggaa atagagatgt aagtggttta aaggggcatt ttaaaagttt atttaaagga
    41461 agtcttacaa caggaggttt taaagcttta tttggtggat ttgtagggct tatagtttct
    41521 gttgctatat caaaagacat aattgatata atagtaaata cactaataat agctctatca
    41581 actaatttaa tgaatctttt ggatttaaga ccaggaaggg caataaaagc atatttattc
    41641 ataatggtaa ttgtattttt aacattgaca ggatttgttc aagttcttcc attacttata
    41701 gtaccaaatg tacttgcata ctttaattat gatttaaaag caagggctat gatgggagat
    41761 actggttcaa acgtccttgg aatatctatt ggaatgctca tttcttttgg gtatccattt
    41821 aacattagat taggatggtt aatatttgta gttattatgc atatattcac tgagaaatac
    41881 tcattaacta agataataga aaaaaataaa ctattaaatt ttatagataa attgggaagg
    41941 tagtattatg ataagcaaaa gagtggggat tgttgaatca atagtaagtc aaactgaaac
    42001 tctagatgat ataagagtaa atataaatgg agaaatacaa aaggcatata attatccaaa
    42061 aatatctgga actataaata taggtgacga agtggtttta aatacaacag cagtagaact
    42121 gagcttaggt acaggtggat accattttgt aataactaat ttaaataata tagaatctac
    42181 tttaactgaa ggtggacata taatgaaact tagatatacg cctcttcaaa taaaagtgga
    42241 ttctgttgag gaacaagaaa gtatttatca tgataaattt gcgaatttta aaggattaga
    42301 aggtctttct gttgtagtag ggactcttca tagtatgtta actccatttg cagcttcatt
    42361 taaaagaaat aatccaaata aaaaattagt gtatataatg actgatggag catctcttcc
    42421 aatttattta agtaaaaatg tggatacttt aaaagaaaaa aaattaatag atagtacaat
    42481 aactatagga aatgcgtttg gtggagatta tgaatgtata aatatatata ctgcattaat
    42541 tacagcaaaa gaaatactaa aagcggatgc agtatttgtc agtatgggac ctggaattgc
    42601 tgggacagga acaaaatatg gatttacagg gattgaacaa ggctctattc ttgatgctgt
    42661 aaaaaaactt gaaggaagag caattgcaat tccaagaatt agttttgctg acaaacgaga
    42721 aagacatcaa ggtatttcac atcatagtat gactgtattt aaagaaattg taaatgtaaa
    42781 tgtagacata ccaataacta tatatgatga tgaaaaattg aactttataa aagagcaaat
    42841 caatttaaat ggtttagaca aaaagcataa tataatttat atagataata ataaaacgaa
    42901 agaggattta gactatttta atctaaaagt aaagagtatg ggaagaaatt atgaacaaga
    42961 tgaggcattt ttcaaagctg caagcacagc agcatattac ttaatggagg tttaagatgg
    43021 tattagaaga aaaaactatt agtagtgata gggtttatac aggtaaagta attactttaa
    43081 aggttgatac tgttgaaatt ccagggcaag gatatcaaaa aagggagtta gtagaagttg
    43141 gtggagcagt aggaatagtt gcaatcacag atgataataa agttgtgtta gttaagcaat
    43201 ttagaaaacc tatagaaaag ccaatctttg aaattccagc tggtaaacta gaaaaaaatg
    43261 aaagtccaaa agaatgtgct gaaagagaat taaaagaaga aactgggtat agtgcaaaaa
    43321 atattaagct aatacataaa tttttcactt cagcaggttt ttctaatgag ataatgtttg
    43381 tatatttagc aacaggtctt actccaggag agaataattt agatgcagat gagtttttag
    43441 atgtttatga aattgagctt gaagaagcat ataacatggt attaaaaaat gacgttgaag
    43501 atgcaaaaac atctattgga ttattattag taaaagatat gtttaaaaat taatatttat
    43561 aaaatgaata accctttgtc ctaaagcata ttattttata aaggacaagg gggaattaaa
    43621 ttgagaagaa catatagaaa aaatgatttt agagaattaa atagacaaat aattattata
    43681 ggattgttgt ttatgatgtc tattgttgtt gggtcttaca taaataaaat attgccaggt
    43741 tctagtaaca atatattaaa caatataaat cctgctgtag agtattataa tctaaatata
    43801 agcataaaag atacagtgat acaaaattta aaatcagatg caatctttat gggctcaata
    43861 gctctattaa gtctatttgt agtaacaatt ccagcagtgt tggtagcttt tgtattaaaa
    43921 gggatgtcaa ttggttatac aataaatagt tgtatattgg cattaaaatt taaaagtata
    43981 aaaatgattt taataatatt atttaaaaat ttgattatta ttccatgtgc aatcatatta
    44041 gcattaatta gtttaagtta ttttaaagaa atggtttatg aatttaaaaa gaaaagtaga
    44101 aaaaatatgc aatttttaat taagaggtat atactaaata ttataattat aatagcacta
    44161 tcattggggt tgcaattaat attaaatact gcaagtattg gtattattaa gttcctagct
    44221 aaataaagga gtttataatg gatattatag agggatatat agattacata aaagataaaa
    44281 aaaaattatc agagaataca gtggcatcat actttatgga tataaaaaaa tatatggaat
    44341 atataaatca aaaaagcatt aaactagtag atatagtaga aaatgatatt ataagttact
    44401 taataaagtt ggaaaaagat aatgtatcaa tagctacaat agcgaggatg atatcctcta
    44461 taaaatcttt tcatgattat ttatttttaa atcatatatg tacaaataat cctgcaaagg
    44521 atataaaaaa acctaaaata aaaaaagaaa atataaatat acttacagaa gaagaaattg
    44581 aaagactatt aaattttcca aagttaacaa cacctaagtt gattagggac aaggctattt
    44641 ttgaagtatt atatggtact ggtataaaag tatctgaatt agtagagatg aatatagagg
    44701 atattgacct agatattgat tatatatatt gtaattcatg ttgtaaaaat caaagagtga
    44761 taccactttg tgatattaca aaactttact tagaaaagta tctaaaagaa gcaagaccta
    44821 aaatggctgc agaaggtgaa aaatcattat tcgtaagttc attaggtcaa aggtttacac
    44881 gacaaggtct ttggaaagtc ataaaaaaat attctaatct agccaatata gataaaaata
    44941 taaatcctac aatgcttaga cattcatttg ctattcattt gttaaatgaa ggtgctaaca
    45001 ttgctgttgt aagtaaaatt ttaggaaatg ttaatttatc tagtttgcaa gtatatttaa
    45061 atcatataga taaaaacgtg agaagagaaa taaaggagaa acatcctaga aatgatgttg
    45121 atgtagaatt acaaaaagca gaagctaaat agactatata gcaaatttta taatttagaa
    45181 ggagcatatt tatatgagca gagtaatttg gattgtaatt gatagtgttg ggataggagc
    45241 attacctgat gctgaaaaat ttggtgatag taaggatgta agtacccttg gaaatatatt
    45301 taaggaatat ccagatattc agattcctaa tatgagaaat ttaggtatag gaaacataga
    45361 aggtatagat ttttttgaaa gtataaaaga accaataggt tgttttggaa agtgtaagga
    45421 aatgtctcag ggaaaagata ctactacagg tcattgggaa atgactggta taatagttga
    45481 caaaccattt aagacatttg aacatggatt ctctaaggag ataatagaag agtttgagaa
    45541 aaaaactgga agaaaagtag taggaaataa accagcttca ggaactgtaa taatagatga
    45601 gtatggagag catcaaataa aaactggaga tgtaatagtg tatacatcag ctgatagtgt
    45661 atttcagatt gctgctaatg aagaagtaat tcccctagaa gaactctata atatgtgcaa
    45721 aatcgctagg gaaataatga tgggggataa tgcagtagct agagtaattg ctagaccatt
    45781 cataggtaag aaaaaaggtg agtttgtgag gacttcaaat agaagagatt attcactaga
    45841 cccatttgaa ccaacagttc ttgataatat aaaagaatca ggacttgatg ttttagcagt
    45901 tggaaaaata gaagatattt ttaatggaaa aggaattaca gatgctatcc acactaaaag
    45961 caatatggat ggagtggatg aaactttaaa ttatatgaaa caagataata aaggtctaat
    46021 atattcaaat ctcgttgatt ttgattctaa atatggtcat aggagagacc cagaaggata
    46081 taagaaagct cttgaagagt ttgatagtag acttcctgaa ataatggcta atatgagaga
    46141 agatgatatt ttaataatca atgcagacca tggaaatgac ccaacatata aaggtacaga
    46201 ccatacaaga gaatatatac ctgttatgat ttatggcaat aagattaaaa aaggttttaa
    46261 tttaggagta aaagatactt ttgctgatat tggagcaact gttgcagata tactaaatgt
    46321 aaagttgcct aaacatgggt caagctttaa agaagattta ttttaaacta taaaatgata
    46381 aaaacttagc ttaataggat ggagagattg gaatgtttga aaagatagca caaagtagta
    46441 aatttataaa ttctaaaagt aatataaaac ctaagattgg tttgatatta ggttctggac
    46501 taggtgattt agcaaatgat atagaagacc ctgtcacaat taaatatagt gaaattccaa
    46561 acttccctgt atcaacagta gcaggacatg ctggacaact tgtaattgga aaacttgaag
    46621 gaaaagaagt tattgctatg caaggtagat ttcattacta tgaaggatat tctcagaaag
    46681 aagctacttt tccagtaagg gttatgaaag aattaggagt cgagattctt atagtaacta
    46741 acgctgctgg aggcgtaaat aaagaattta aagcaggaga tttgatgctt ataagagacc
    46801 atataaactt tagtggaagt aatccattag taggaaaaaa tgatgacaga tttggtgcta
    46861 gatttccaga tatgtcagat gcatattctt ctaaatatgt agatgtagtt aaaaactgcg
    46921 ctaaagaatg caatatagat gtcaaagaag gcgtttatat gttttttagt ggtccaaact
    46981 atgaaacacc agcagaagtg agaatggcac aaattttggg agctgatgct gtaggtatgt
    47041 ctactgttcc agaagtcata gtagcttctc actctaatat aggagttatt ggtatatctt
    47101 gtataacaaa tatggctgct ggcatcttaa atcaaccact aagtcatgag gaagtaatag
    47161 aaactactca aaaagttaaa tcagaattta tgaatttagt taaaacaacg gttaaaaatt
    47221 tataagttaa agcttattat agattttttg aggaagttta taataaaata gttaatgttt
    47281 aatttgaaaa gaattaataa aaagctgtct tgatatagaa agttatttct aataaaggac
    47341 agttttttta taatactaca acatttgaaa gtgttttaaa gtttaatatt tatttaaatt
    47401 attataggtt agaatggaga tttcaattat gagaatgtat gatattataa aaaaaaagag
    47461 agataaccta gagcttagta aaaaagagat aaactacttt atagaaaact attcaaaggg
    47521 agatatacct gactatcaag cttctgcact attaatgtcg atttatttaa ataagatgaa
    47581 taaacaggaa actgtacatc ttacagaagc aatgatgaac tctggagaca tgataaacct
    47641 atctgagata gatggaataa aggtagataa gcatagtact ggtggtgttg gagataagac
    47701 taccatagct cttatacctt tggttgcttc atgtggagca ccagttgcaa agatgtcagg
    47761 tagaggtctt ggtcatactg gtggtactat tgataaattg gagtctatac caggtttttc
    47821 gacagaaatg gagataagta aatttattga ttctgtaaat aagtctaaaa tagcagtttg
    47881 tggtcaaagt gcaaaggtag caattgcaga taagaaaata tatgctctta gagatgttac
    47941 agcaactgtt gataatatat cattgatatc atcaagtgta atgtctaaaa agttagcttc
    48001 aggagctgat gcaattgtgc tagatgtaaa aactggtgat ggagcattta tgaagacatt
    48061 agaagagtct tttgagttag ctaaatctat ggttgatata ggtactggta tgaatagaga
    48121 tacaataggg attgtaacag atatggatga acctttaggt tttgcagtgg gaaactcttt
    48181 agaagttata gaggcaattg agacattaaa aggaaatgga cctaaagacc ttgttgagct
    48241 atgcgaagta ttaggagcat atatgttggt tttatcaaag gtagcacatg attttgaaga
    48301 tggtgttgaa aaaataagag aatcaataaa atctggtact gctatacaaa aactcaaaga
    48361 gtttatagag aatcaaggtg gtaataaaga tgtagtagat gattattctt tattcaaaaa
    48421 atcaaagtat aaatatccag ttaaatcaac taaatcaggt tatataagca aaattaaagc
    48481 tgaagatgtt ggtgtatcgg ctatgattct tggagcaggt agagaaacta aagatgatat
    48541 tttagattta tcagcaggga taattttaga aaagaaagta ggagattatg taaatgaagg
    48601 tgaagtttta gcttacatgt attataatga tgaacaaaag ctttcattgg caaagggaag
    48661 atttattgat tcctactcta ttgtagattc aaaaatagaa aaaaataaat taatatatgg
    48721 tattgtaact aaagaagaga ttaaaaagtt ttagaaaatt ttagaaaagt gattataaaa
    48781 gg
//
LOCUS       DAMJZE010000011        48480 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJZE010000011 DAMJZE010000000
VERSION     DAMJZE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.011"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            156..1469
                     /locus_tag="PC541_000535"
     CDS             156..1469
                     /locus_tag="PC541_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2838015.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            1641..2816
                     /locus_tag="PC541_000536"
     CDS             1641..2816
                     /locus_tag="PC541_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2838016.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2919..3938
                     /gene="hrcA"
                     /locus_tag="PC541_000537"
     CDS             2919..3938
                     /gene="hrcA"
                     /locus_tag="PC541_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2838017.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3968..4588
                     /gene="grpE"
                     /locus_tag="PC541_000538"
     CDS             3968..4588
                     /gene="grpE"
                     /locus_tag="PC541_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2838018.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4680..6527
                     /gene="dnaK"
                     /locus_tag="PC541_000539"
     CDS             4680..6527
                     /gene="dnaK"
                     /locus_tag="PC541_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2838019.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6738..7892
                     /gene="dnaJ"
                     /locus_tag="PC541_000540"
     CDS             6738..7892
                     /gene="dnaJ"
                     /locus_tag="PC541_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2838020.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8858..9793
                     /locus_tag="PC541_000541"
     CDS             8858..9793
                     /locus_tag="PC541_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2838021.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9829..11202
                     /locus_tag="PC541_000542"
     CDS             9829..11202
                     /locus_tag="PC541_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2838022.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11390..12157)
                     /locus_tag="PC541_000543"
     CDS             complement(11390..12157)
                     /locus_tag="PC541_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2838023.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDFI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12187..13296)
                     /gene="ugpC"
                     /locus_tag="PC541_000544"
     CDS             complement(12187..13296)
                     /gene="ugpC"
                     /locus_tag="PC541_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2838024.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            13554..14291
                     /gene="cas6"
                     /locus_tag="PC541_000545"
     CDS             13554..14291
                     /gene="cas6"
                     /locus_tag="PC541_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2838025.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            14296..15771
                     /locus_tag="PC541_000546"
     CDS             14296..15771
                     /locus_tag="PC541_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838026.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            15791..16804
                     /locus_tag="PC541_000547"
     CDS             15791..16804
                     /locus_tag="PC541_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HDF2838027.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            16820..17617
                     /gene="cas5"
                     /locus_tag="PC541_000548"
     CDS             16820..17617
                     /gene="cas5"
                     /locus_tag="PC541_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2838028.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            17644..20052
                     /locus_tag="PC541_000549"
     CDS             17644..20052
                     /locus_tag="PC541_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2838029.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            20493..21440
                     /gene="prmA"
                     /locus_tag="PC541_000550"
     CDS             20493..21440
                     /gene="prmA"
                     /locus_tag="PC541_000550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2838030.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            21459..22217
                     /locus_tag="PC541_000551"
     CDS             21459..22217
                     /locus_tag="PC541_000551"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2838031.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            22219..23517
                     /gene="mtaB"
                     /locus_tag="PC541_000552"
     CDS             22219..23517
                     /gene="mtaB"
                     /locus_tag="PC541_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2838032.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            23560..23910
                     /locus_tag="PC541_000553"
     CDS             23560..23910
                     /locus_tag="PC541_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2838033.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            24073..24252
                     /locus_tag="PC541_000554"
     CDS             24073..24252
                     /locus_tag="PC541_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2838034.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            24282..24725
                     /locus_tag="PC541_000555"
     CDS             24282..24725
                     /locus_tag="PC541_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2838035.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            24836..25333
                     /locus_tag="PC541_000556"
     CDS             24836..25333
                     /locus_tag="PC541_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2838036.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            25526..26089
                     /gene="raiA"
                     /locus_tag="PC541_000557"
     CDS             25526..26089
                     /gene="raiA"
                     /locus_tag="PC541_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2838037.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            26302..26538
                     /locus_tag="PC541_000558"
     CDS             26302..26538
                     /locus_tag="PC541_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2838038.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            26551..27894
                     /gene="yqfD"
                     /locus_tag="PC541_000559"
     CDS             26551..27894
                     /gene="yqfD"
                     /locus_tag="PC541_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2838039.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            27833..28948
                     /locus_tag="PC541_000560"
     CDS             27833..28948
                     /locus_tag="PC541_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2838040.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            29005..29466
                     /gene="ybeY"
                     /locus_tag="PC541_000561"
     CDS             29005..29466
                     /gene="ybeY"
                     /locus_tag="PC541_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2838041.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            29472..30191
                     /locus_tag="PC541_000562"
     CDS             29472..30191
                     /locus_tag="PC541_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2838042.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            30310..30711
                     /locus_tag="PC541_000563"
     CDS             30310..30711
                     /locus_tag="PC541_000563"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2838043.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            30775..31668
                     /gene="era"
                     /locus_tag="PC541_000564"
     CDS             30775..31668
                     /gene="era"
                     /locus_tag="PC541_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2838044.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            31680..33059
                     /gene="mgtE"
                     /locus_tag="PC541_000565"
     CDS             31680..33059
                     /gene="mgtE"
                     /locus_tag="PC541_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2838045.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            33132..33287
                     /locus_tag="PC541_000566"
     CDS             33132..33287
                     /locus_tag="PC541_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2838046.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            33253..34026
                     /gene="recO"
                     /locus_tag="PC541_000567"
     CDS             33253..34026
                     /gene="recO"
                     /locus_tag="PC541_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2838047.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            34019..34618
                     /locus_tag="PC541_000568"
     CDS             34019..34618
                     /locus_tag="PC541_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2838048.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            34778..35656
                     /gene="glyQ"
                     /locus_tag="PC541_000569"
     CDS             34778..35656
                     /gene="glyQ"
                     /locus_tag="PC541_000569"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2838049.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            35656..37722
                     /locus_tag="PC541_000570"
     CDS             35656..37722
                     /locus_tag="PC541_000570"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2838050.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(37888..39435)
                     /locus_tag="PC541_000571"
     CDS             complement(37888..39435)
                     /locus_tag="PC541_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2838051.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            39715..40482
                     /locus_tag="PC541_000572"
     CDS             39715..40482
                     /locus_tag="PC541_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2838052.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            40765..40962
                     /locus_tag="PC541_000573"
     CDS             40765..40962
                     /locus_tag="PC541_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2838053.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            40991..42067
                     /gene="vorB"
                     /locus_tag="PC541_000574"
     CDS             40991..42067
                     /gene="vorB"
                     /locus_tag="PC541_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2838054.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            42070..42789
                     /locus_tag="PC541_000575"
     CDS             42070..42789
                     /locus_tag="PC541_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2838055.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            42802..43341
                     /locus_tag="PC541_000576"
     CDS             42802..43341
                     /locus_tag="PC541_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2838056.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            43498..44556
                     /gene="buk"
                     /locus_tag="PC541_000577"
     CDS             43498..44556
                     /gene="buk"
                     /locus_tag="PC541_000577"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2838057.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            44574..45467
                     /locus_tag="PC541_000578"
     CDS             44574..45467
                     /locus_tag="PC541_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2838058.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            45497..46705
                     /locus_tag="PC541_000579"
     CDS             45497..46705
                     /locus_tag="PC541_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2838059.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            46729..47988
                     /locus_tag="PC541_000580"
     CDS             46729..47988
                     /locus_tag="PC541_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2838060.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        19315 a         5073 c         8816 g        15276 t
ORIGIN      
        1 agaatatttt agaagttacg attgtatgat ttttaaatgc ataggcattt actctaggac
       61 tatagactaa tataaaaaaa aactttggga atactaaaac cataaattta taaaattaat
      121 attttatata ctgtaaaaca gtgaggaggg acagtatgca tactcaacta caaaagacta
      181 ttggtctttc agcagctctt tctactgttg taggtatggt tataggttcg ggagtttttt
      241 ttaagccaca agcaatatac actacaacta atggagctcc aggtctaggt attatagcgt
      301 ggcttttagg tggttttata acaataactg ctgggcttac agctacagaa atttcagctg
      361 ctataccaaa aactggtggt atgatgattt atatcgaaga aatatatgga gaaaagttag
      421 gttttttaac tggatggatg caaactgtat tatttttccc aggtacatca gcggcattag
      481 gagtaatatt tgctcaacaa gcatctgaac ttttaggaat gagcccaaat aatatggcaa
      541 atgtacttcc tatagcaatt ggagttatac tatttttagc gttattaaat ataataggtt
      601 catccttagg aggaaaagtt cagacagtag ccactattgg aaagatgata cctttaattt
      661 taataatagt ctttggattt attaaaggtc aaagtagtga agtattaaat ccatttgtag
      721 gagatggtgt aaatgcatca aatgcacttg gacaagtatt aatagctact ttgtttgcat
      781 atgatggttg gataaatgtt ggagctatat caggagaaat gaagtctcca gagaaagatt
      841 taccaagagc aatagttggt ggtttatcat tagttatggc agtttatatt ataataaata
      901 tagcttatct ttgggtagta ccagcaagtg aattagcaac agttacttcc ccagctacat
      961 tagtagcaac aagattgttt ggaaatatag gtggaaaagt aataactgtg ggtatattaa
     1021 tatcagtatt tgggacattg aatggatatt tattaacagg ttcaaggata ccttatacat
     1081 tagctgaaat gggaacttta ccagcatcta aaactctttt aaaagtaaat tctggaggtt
     1141 ctcctgtaaa ttcaatacta cttataactg tgttagcctg tgtatatgca ttatctggtc
     1201 aatttaatct tttgacagac ttaacaatat tttcaatatg ggtattttat gtattgactt
     1261 ttataggtgt tatgaggctt agaagacaaa agccagattt acacagacca tataaagtcc
     1321 ctttatatcc aataatacca attatagcta tattaggtgg actgtttgta ataataaatc
     1381 aaatattgac ttcaacagtc atatctttag gcggaatatt tataacacta ttaggtttac
     1441 cagtttatta ttatatgaaa aaaagataac tacttaattt aagttagata aatttgggta
     1501 aaggtgaaaa ttaactttta tccaaatttt ttatactgtt atacaatata agtattatat
     1561 attgcttgat gtgctatttt gtttgattta taatttagta attgatgttt taagataatt
     1621 taagaaatga ggagtgaaag atgctaggac tttatgtaca tattccattt tgtgtgaaaa
     1681 aatgtaagta ttgtgatttt aattcttata aaatggacat agattcaaaa aaaagatata
     1741 tagaagactt aaaaatagaa atggaattat atagtaataa attatataaa gataataaat
     1801 ataaaaataa agaatgctgt agtctaaata aaaatgacaa aataacgagt atatttgtag
     1861 ggggaggtac acctagtata ctaacctctg atgaaataag agaagtattt ataagtataa
     1921 aagaaatgtt tgatatagat gagaatgcag aaataactat agagtgtaat cctgggacat
     1981 taacattaga aaaattaaaa actatgaaag agattggcat aaatagactt agtatagggc
     2041 tacaagctat tcaagaaaag catttgaact ttataggaag aatacatact tatgaagaat
     2101 ttgagaaaaa ctataaagat gcattaagtg taggttttaa aaatattaat atagatttaa
     2161 tgtacagtct tccaaatcaa actctatgtg attggaagga aactcttgaa aaagttgtac
     2221 acttaaatcc aacacatata tcagcttatt cccttatact agaagaagga actgaactat
     2281 acaatatgta tgaaagtaat aaatttgaac ttatagatga aaatgtagat atagaaatgt
     2341 atgagtatac aataaactat ttaaagtcaa aaggatataa tcaatatgaa atatcaaact
     2401 actcaaaaga aggctataat tgtgagcata atattttata ttgggaatgt gaacattata
     2461 tagggattgg agcaggagct tctggctata ttaatgagaa cagatacaat aatgtagaat
     2521 ctttagaaga ttatcattta agtttggtaa aaagagaaaa accaatacaa gaaaatgaaa
     2581 ttctttctga gaaagatatg atagaagaaa agatatttat ggggcttaga atgaataaag
     2641 gcataaaatt tgaagatttt aaaaaaaaat ttggaataga ttttagagag aagtataata
     2701 agcaaataga aatgctatta gctagaaagc ttataaatca gagctttgaa ggtatacaat
     2761 taactcaaaa aggaagagaa atatctaata gtgtatttat agaatttatg gaataatttt
     2821 taaggtgttg acaaaataaa tttataatgg tatataatat atataatctt tttagcactc
     2881 gaacttagtg agtgctaaca gctaggaggt ataataatat ggaattaaat gaaagaaaat
     2941 taaatatcct caaagctata gttaaagatt atatagaaac agctgaagct ataggttcta
     3001 gaacaatatc taaaagacat gatttaggtg ttagtgctgc taccataaga aatgaaatgg
     3061 ctgaccttga agaattaggg tatttaattc agcctcatac ttctgcgggt agagttccat
     3121 ctgaaaaagg ctataagtta tatgtaaact ctcttatgag taaaagcgaa ttggatgata
     3181 atgataagat tcttattgaa caatgtatga atcataatat aaatcatata aaagaattga
     3241 ttcatgaaac ttctaagcta ttatcgcaac taacaaacta tacaactgta gcagttacta
     3301 agagtttaat aaatcaaagt gttataaagc acatacagtt ggttgctatg aatgacaata
     3361 atattttact tatagttgta acagataaag gggacttaaa aaaagctaac cttacaacta
     3421 atgtatattt agatcaatct aagttaaatt tgatttctga taatcttaca aggaagctct
     3481 taggtaaaag tataacagat ttggatgata atctaattgc ttttattaaa tacgaaataa
     3541 gtgagtattc tgggcttata gatgagcttt taaatgcttt gaactcaaat atgaaagaag
     3601 aagatttttc tctatcatta aatggagcta ctaacatatt tagttatcca gaatttaatg
     3661 atgtattaaa agcaaagtca tttttaaata tgttggaaaa aaaggaaaca atagctgaca
     3721 taattaaatc aaagggaata caaaaagaca atctaaatat aataattggt agtgataatg
     3781 attgtgaact ggcacaggat tgtagtatag ttacagcaac ttataatgta gatagagact
     3841 tggttgggcg aataagcttt ataggcccta ccagaatgga ttatgctaga atttactcta
     3901 taataaatta tatgagttta ttaattaata gaaagtaaag aaattttaaa ttgcgaggtg
     3961 taaagtcttg gataaaaaaa atgagcaaca ggaagtaaga gaagaaaatg atacttctat
     4021 taaccaagaa tcggaaactc aagtagagtt agaagaggaa gttgttaatg aagaatgtga
     4081 aacatcaagt gaaaaaactg atgaaaaaga agttgatgat gaaaatgtaa cagatataaa
     4141 ttctaagtta gcagaaaaaa aattgcagga tgagttggat gaattaaatg ataaatatca
     4201 aagacttcaa gccgaatatg caaactacag aagaagaact caacaagaga aagagacaat
     4261 aggcgtattt gcaaatgaaa aaataataac tgaattgata ccagttatag atagtatgga
     4321 aagagcctta gatgcatgtg aagataagga agatactatg tataagggta taagccttgt
     4381 acataaacaa ttaatagaca ctttagtaaa atttggagta gaagaaatag aagcagaatc
     4441 taaggaattt gaccctaatc tacatctagc agttatgcaa gaaagtgtag atggagttga
     4501 agcaaatcaa atagtaatgg tacttcaaaa aggatataag ctaggaacta aagttgtaag
     4561 accttcaatg gttaaagttt cttgctaagt ataaattaaa ggttaaatta tttaacctaa
     4621 tataaaatat taaaataagt aaaacttaat tatataatta ctcgaggagg attttagtca
     4681 tgggaaaaat aataggaata gatttaggaa caacaaattc atgtgttgca gtgttagaag
     4741 gtggagaagc acaaataata gcaaatagtg aaggtatgag aactactcca tcagtagtag
     4801 catttacaaa agatggtgaa agaatagttg gagaacctgc aaaaagacaa gcagttacaa
     4861 atgcagataa aacaataact tctataaaaa ctcatatggg aactgattat aaagtaaata
     4921 tagatggaaa atcatatact ccacaagaaa tatcagcaat aatattacaa aaattaaaat
     4981 cagatgcaga aagttactta ggacaaacag taacagaagc agttataaca gtaccagctt
     5041 actttacaga tgctcaaaga caagcaacta aagatgctgg tagaatagca gggcttgatg
     5101 ttaagagaat aataaatgag ccaacagcag cagctcttgc ttatggtatg gataaattag
     5161 accaagaaaa gaaaatatta gtatttgact taggtggagg aacttttgac gtatctatac
     5221 ttgaaatagg agatggaact tttgaagttt tagctacagc tggtaacaat agattaggtg
     5281 gagatgactt tgaccaaata gtaatagatt atttagcaga ggaatttaaa aaagctgaag
     5341 gtgtagattt aagaaatgat aaaatggctc ttcaaagatt aaaagaagca gcagaaaagg
     5401 ctaagaaaga attatcatca acaatgagtt caaacataaa cttacctttc ataactgcta
     5461 cagcagaagg accaaaacac ttaaatatag atttatcaag agctaaattt gaagaattaa
     5521 ctagaggttt agttgaaaaa actatggaac caactaagac agctttacaa gatgcaggac
     5581 tttctacagg tgatatagat gatgtattat tagtaggtgg ttctacaaga ataccagcag
     5641 tacaagaagc tgttaagaag tttataggaa aagaacctca taaaggtata aacccagatg
     5701 agtgtgttgc agcaggtgct tctatacaag ctggagtttt agcaggggat gttaaagact
     5761 tattattact agatgttact ccattatctc ttggtataga aactatgggt aatgtaatga
     5821 ctaagataat agaaagaaat actacaatac caactaagaa gtctcaaata ttctcaactg
     5881 ctgctgataa ccaaacagct gttgatatac atgttcttca aggtgaaaga agtatggctt
     5941 atgacaatac aactttagga agattccaat taacagatat accaccagca caaagaggaa
     6001 tacctcaaat agaagttact tttgatatag atgcaaatgg tatagttaat gtttcagcta
     6061 aagatttagg tacaggaaaa gagcaaaaaa taactataac atcaaacact aatttatcag
     6121 aagcagaaat agagcaaaag ataaaagaag cagaaatgaa tgcagaagct gacaaacaaa
     6181 agaaagaaaa aatagaagca ttcaatcaag ctgaatctac tatatatcaa actgaaaaaa
     6241 ctttaaatga acttggagat aaaataagtt caggtgaaaa agaggatata gaaaaagcta
     6301 tagcagactt aaaagctgta aaagataatc aagatgctac agcagaagaa cttaagaaag
     6361 ctacagatga agtaatgact aagttccaaa aagtatctca agaaatgtac caaaaagcag
     6421 ctcaagaaca acaagcggca caaggtgcag aacaagcaca agacaatgga cctaaagatg
     6481 acaatgtagt agatgctgac tttaaagaag tagatgaaga taaataagct atataatatg
     6541 taacactaaa tgataattgg aacatatatg atttgtgaaa atcatataga tgttaaagat
     6601 atatggacaa tatacatcat aaagaatata atatatcata gattatataa cttaaagtag
     6661 aatataagaa ttaatgatag cttgtagttt attattctac aagctatcgt tttgtaaaat
     6721 gaaggtggtg tcaatttttg agtacaaaaa gggattatta tgaagtctta ggtatcagta
     6781 aaggtgcaga agctcaagag ataaaaaaag cttatagaaa attagcaatg aagtaccatc
     6841 cagatagaaa tcctggagat aaagaggcgg aagaaaaatt taaagagata aatgaagctt
     6901 atgaagttct atctgatgat acaaagagaa aaacttatga ccagtttgga catgatgggc
     6961 taaatggtca aggcggtttt ggtggtcaag gaggatttgg cggtcaaggc tttggtggtt
     7021 ttgaagatat gttcggagat atatttggtg acatgtttgg aggaagcttc ggaggtggaa
     7081 gagcaagaag aagagggcct caacgtggtg cagatataag acagagtgtt actatatctt
     7141 ttgaagaagc tgcatttggt aaaaagatgt ctataaaagt aaatagaagt gaagaatgtg
     7201 aagaatgtaa tggaactgga gcaaaaccag gaacatcaaa gaaaacttgt tcaacttgta
     7261 atggaactgg tcaagttaga actgttcaaa gaactccatt tggtaatata gcaagttcaa
     7321 gaccatgtag tgcttgtaat ggaactggag aagtgattga atctccatgt agtaaatgtc
     7381 atggaactgg aaatacaaga aaagttaaga ctatagaagt agatatacct gctggtatag
     7441 atgatggtca aatgataaaa ctttctggtc aaggtgaagt aggagaaaaa ggtgctccaa
     7501 gaggagactt atatattgta gttaatgtta agtcacatcc actatttact agagatggaa
     7561 atgatattta ttttgaaatg ccaattacat ttgttcaagc aactttaggt gatgagatag
     7621 aagtacctac tttggatgga aaagttaaat atagtgtgcc agaggggaca caaactggaa
     7681 ctgtatttag acttaaagag aaaggaatcc ctagaattag aggtaattca agaggagacc
     7741 aatatgttaa ggttgtagtt gaaattccta aaaaattaaa cgataagcaa aaagaactat
     7801 tgagagaatt tgcgaaggag tgtgggtcga acgtccacga aaagaagaaa accttcggac
     7861 aaaagataga agatatgttt aagaaaaagt agttaaagta gccaatatat aatcatattt
     7921 ttaaaatctt agttatttat aagattgtca aaggagatat tatgattttt atattggcta
     7981 tttttgtata aaaaataaat gtgcataaaa taaaactgaa tataaataat tttctgaaaa
     8041 atacttgtcc taaaatatat aatatgctat aatataatca aataaaagaa aggggtgatt
     8101 ccagtgggaa cggatgtggc actgccaaat gatgaagaaa actttattgt aatatacaaa
     8161 ataaaattag atacttttag tgctagatta agtactggaa aaactcatga gctgggataa
     8221 ttattgtaat tacctagact caaactttta gtagtaaaat agtaatatta tatagacaag
     8281 gtatctaatt tttaaatctt ttgtatgaat taaaattttt ataaagggta tatctgatta
     8341 gaatgtaatt agggaaacct agttgtgaat tacttttagt atatacatgt gtaaagaaat
     8401 atgtatatgg tattggttat tgtgctagat gtgtatgtaa aggaatatgt gtctagttgg
     8461 gtatatgtaa agaaatatac ttataatcta tgaaaaattt ttagtataaa ttgctaaaac
     8521 attgagattg ttttttatag aaagttagtt tataccaact aaaaatgtgc ttaagatttg
     8581 tttgatgttt aattataaat aaggttgtag ataaaaaaat aagtaaagac aaaggtttaa
     8641 aatacttgct ttaaatatag tttgttttga acacttgcca aattaaaata attaataaaa
     8701 tagacatctt ctcatagtaa aatatactgt gagaggatgt ttttgctttg ttcataaact
     8761 tttatgaaaa atttgcatag tgtcacattt taatatacta tatatataat aaaatataga
     8821 taaaattaaa tgattcaaaa gagaaaggtg aaaagagatg tattatatag gtgtagacat
     8881 aggaggaaca ggtatacaag caggagtagt tgataactat gggaaaataa tttttaggtc
     8941 agagtgtaaa acagtaattg aaaaaggctt tgaaggaata cttaatgaca taaaaataat
     9001 gatttataag ttgttggaag ataataaact tactatgagt gacataaaat ctattggatt
     9061 tggtgttcca ggttttataa ataaagaagg attagtaact tgtgtaaatt taaaatggaa
     9121 taaaaaagct tttaataaag agttaaaaag aagatttcca gatgttgaaa ttcatggaga
     9181 aaatgatgca acagtagcag cacttggaga agcaaagttt ggaagtatga aaggtgctaa
     9241 tgttggagta ttatatactt taggaactgg agtaggtgga ggaatagtaa ttaatcaaaa
     9301 agtcttttct ggagcacatg gattgggttc agaaataggt catcaaatta taggagaaaa
     9361 ctattttaat tgtaattgtg gaaataatgg ttgtgtagaa actttttgtt cagcaactgc
     9421 aattataaag tattctcaaa aactgataga agaaggagaa aagagtagga ttttagattt
     9481 ggcagaggga aatttagaaa atgtaaatgc aaagatggtg tttgatgcat atagagaaaa
     9541 tgatttagtg gcaattaaag taataaatag atttaaagag tatttagcaa aaacttttgc
     9601 aaacacaata aattctttag acccagaaat tatatctata ggaggaggca tttctaaatc
     9661 tagcgatata atacttgatg gaatagaaga tttggtaaga aaatttgtat tatacaaaac
     9721 agaagatata gctacaatta catgtgcaac tttaggttca gacgcaggaa ttataggagc
     9781 agctttttta taatgtaaat gaactgctta ttggaggcgt gatgatatat gggtaaaaca
     9841 aatgctagaa gaacgctttt tttgatagca ataggttcag gaactatgct gaatcctctt
     9901 aattcttcta tgatttcact tgctttacat agtatacaaa atgattttca tattagtttt
     9961 acaactgtat cttggattgt ttcagcattt tacctagcta gtgctattgc acaaccagtt
    10021 tcaggtaaga taggagattt aataggtaga aaggtcttat ttttgagtgg tttgatgctt
    10081 gtaattttat cagcatccat agtaccactt gttcaatcat tctttatatt gatttttata
    10141 agagtaattc aagcaattgg aacaagtacc ctatatccat ctggagttgc tcttatacaa
    10201 aataatataa aagaaagaca atcttctgca ttagcagttc taaccatttt tgcttcaact
    10261 acagcaggat taggtccgac acttggagga ttattgttgg acttaggagg atggcatgct
    10321 atatttcttg taaatatacc tgttgttttg gtttctatat gcttagggta ttttttattc
    10381 ccaaaagaag taaaagaaaa gaaatctata aaagaaactt taaaaaatgt tttgtcaaga
    10441 ttagatatta ttggcatact tctattttca attgcaatga tttttatatt actatttttg
    10501 ctatctatga aggagtcttt taacttggaa caacttgtat ttggtataat actgatgtgt
    10561 ctttttattt ggcatgaatt gagaacaaaa ttgccattta tagatattaa gctttttgta
    10621 agtaatccta aattgtcaaa agtttacttg caatttatta tattaaattt ctttaattat
    10681 attttgtttt ttggtcttcc tagttatttt caagatgcct tacattatag tgcaaagtcg
    10741 actggattat ttatgctatt tatgtctggt ataggtatat ttatatctcc attaactgga
    10801 aaatggatag ataaatctgg aactagattt cctcttgtta catcatcaat ctttatgttt
    10861 attagtgcag tacttctttc agtagttttt atccatatac ctgtaatagg aaagggtatt
    10921 atattatcat tagcaggtat tagttatgga gttggtaatg tagccctgca atcctctatg
    10981 tttgaagaaa gtcctcgtga cagtataggg acagcttctg gtctgtttca aactagtcgt
    11041 tatttaggtt ctattttagc aactgtttta cttggaatgg tatttgaagt aaatataaca
    11101 tcagaacaat ttcagatatt aggttatgtt atggtggtct taggtacaat aagtttttta
    11161 ttaaatttta tattaaagaa agaattaaga aaagtagaat agtggattta tggatttaat
    11221 aagtctaata actgataagt ttaaaaatat tgtttgtaaa acataactgg tttgatattt
    11281 attaattcta agttttgaag catagagtac tcgataagta tacaatattt tgaattaaaa
    11341 ttaagcagaa atttgaacta gtctttcaaa tctctgctta atttagtagt taaatttttt
    11401 tatagagaaa ataagatttg acacttagat aaaaaatcat tgcattttca aaattcttta
    11461 aatcaaaccc aagtatctca ttaatctttt ccaatctata tactagcgtg tttctatgta
    11521 aaaacatact agtagatgtc ttagaaatat ttaaatcatt atttataaat tttattcctg
    11581 tatatagaag ttcttcgtca atagatttaa tcttattaaa atctattaat ttattgagta
    11641 tattatcctc attaattaaa tctaaagttt tatatctaat aaagtcaaac aaatttaaaa
    11701 acttattttc agcgttgtct tttataatat gaggtagtaa atcagaacat aactgtaatc
    11761 taaaatcaaa atcagcttta ctatctaatt caccaagata aaaaatataa gtactttgat
    11821 aagtttcagt ttcaattata ttttgtgtag ttacattaat atctaagtta tatttagaat
    11881 ttactataaa aaaaccattg tgttgtatta tatttatatc ttcaaataaa ttataaaaaa
    11941 tattttttag atattcaaag tctatatgag attggtaaat taaataaaag ttgcctttta
    12001 aattatcgag attattttta tctagaaaat caataacata aatagcatta ttgaaagtaa
    12061 ctaaatggct actagcatct aacttttggt tttcatatgg atagatattt atttttttat
    12121 taagctgtct ttctaaaaaa tcttttaact cttccataaa gtctccttaa aaataagatt
    12181 atattcttat cttatagcta aagtagtttc tttgtcaaat atgtgtactc tactattgtc
    12241 aactgaaagt gttaagtcat caccaacatt aattctgttt ttactgtcaa atctaattgt
    12301 tatcgtattt tcgcctagtt ttaagtgagc ataagtttct gctcccataa gttcttttat
    12361 ttcaattctt gatttaaaac aagtatctgg attagattta ataaaagtat cttctacatg
    12421 aatatcttct ggtctgatac ctataacaac ttcattatta atataattgt cttttattaa
    12481 atcatagtgt ttttctttat tcaatttaag cttaatatgt tcattttgtg cataaatttc
    12541 atcattttct tctattaata ttacatcaat aaaattcatc tgtggtgctc caataaatcc
    12601 tgcaacaaat atattagctg gataatcgta tacatcttgt ggagttgcaa tctgctgaac
    12661 tactccatcc ttcattacta cgattctatc agccatagtc atagcttcaa cttggtcgtg
    12721 tgttacatag ataaatgtag ttccaagctg ttggtgtagc ttagttatct ctgttctcat
    12781 agcagttcta agttttgcat ctaagttgga tagaggttca tccattaaga ataccttagg
    12841 atttctaact attgcacgtc ctaaggcaac cctttgtctt tgtcctccag ataatgtttt
    12901 aggtttttta tttaaaagag gtagtaagtc taatatttta gcagcttctt taactttttt
    12961 gtctatttca tctttaggca aatttctaag tttaagtgca aatgccatgt tttcgtaaac
    13021 agataagtgg ggatataaag catagttttg aaaaaccata gctatatctc tatcttttgg
    13081 ttctatatta ttgacaagtt tatctccaat gtatagttct ccttcagaga tttcttcaag
    13141 accagcaatc attctaagtg tagtcgattt accacaacca gatggtccta ctaaaacgat
    13201 aaattcttta tctttaatgt ctatatttat attttttact gcgttaaatc catttgagta
    13261 aagttttgat atatttttta aaattacttc tgacataata tcctccttga tatttaaatt
    13321 ttaattatat agtatcaaat ttttttatta tattaaatgt gtaaaatgac taaatgtatt
    13381 tgtttaatat gtatatttag tttttagcaa agaataaaac taacagtaga atatatttaa
    13441 aaaattaaaa aacaaaatta aatttattat caaaaaatca ttttatgata tgttttattt
    13501 ttatattaca aatagagaat aatgaattat actagaaagt aggaggtgaa aatatgagca
    13561 gaatttcaat ttttatggaa tcatgtaatg gcaaatttcc aaatgaaaat tcaatgttgt
    13621 cagtttcctt tataaaaaat attttaaatg cagaaaacaa agattttgct aaaaacatat
    13681 ttaactatgg ggaagaaaga aaaaacaata agcaaataaa ggatataaat acagccattt
    13741 acataccaaa tcttataaga agcaaaaatg agttacttgt tgatggagat ataatattca
    13801 atataagttt ttataattat tctatgttca gtaaattata caatggaatt ttaaaagtaa
    13861 aggagctaga atataaaggt tatagattta aaataaaaaa tataaaaata cataaagaac
    13921 acgagataaa agaaaatggt gttatattta aaactatgtc tcctataata gtaaagaata
    13981 aagaaggtaa atatttagat gtagaagatt caaattatat agaatgttta aactatatag
    14041 ctaatctaac tctggagagt ataagaggta gtgggcttag aaagccattg gaatttatac
    14101 ctattaactt caaaaaaaga gtgttaaaag aaaagattcg tggatttaaa gtgagagaat
    14161 attactatat aaatgcttat gcaggaacat tttttttaaa aggagatatg gaagatttaa
    14221 atgcactgta taaaatggga attggatata gaaggacaga aaatgcagga atggttgata
    14281 tattaaagta gcgaggtgag agaaataaaa gctaaatttt atagatcagg tgatattgta
    14341 agagatattg gcattgttta cttttatgaa ttactaatgg atttaaaaaa cgaactagaa
    14401 aaaaatgatt ataagctaaa atttacttgt gagttaaata gaaattattt atctttagaa
    14461 tctgcagaat atatagaccc taagtatata tctgattata tattagaaaa tcgacttttt
    14521 aaggtattta aaaatgggaa aaaggaaacc ttagaaaaaa tatttagtaa tatagataat
    14581 ttaacgtatg taaattttat tgaagaatta gataaatctt ctgcaactgc taaacaaaag
    14641 gaagatatta aaaaagattt taaaaacagg aggtttccat atgttagaaa tagtggtaag
    14701 tatggattaa acacgagttt agaaaatatg gaaacaaatg taaaaagaat agtagatact
    14761 gtaattaaat caaatataga gatagatgaa atagataaac ttgatttgac acaatatgaa
    14821 gaaaaagata gtgtatgtaa tgtttgcaat ataaataaaa caacaaaact agatattgat
    14881 ttaagagaac gaaaggactc taagtataac tttttattta gaggtgcaga aaaatcaggc
    14941 tttaaaagaa gtggacaagt tgaaagtaat atttgttttg agtgtgaatt tttcaactta
    15001 atgtgtcttt tatatataaa tttaaaaaga ccaatggttt ttgcatatac gaatgattta
    15061 agagaattag catttttaaa tcataagata atgcttaaaa gaaaaatgta ttcagataaa
    15121 tcattttata aaaaactttt gcatgaaaaa ataagttcaa ttagactata tagctttgat
    15181 attgatacga ataaaggtat aattctaaag tttgattcta ttatagagta taaagaatta
    15241 ttaaaaaaaa ttgaacttat agatattata gataattata atttttcaag agagccaggt
    15301 aatactagaa atttgggtaa aagaatgatt gataatggga atttatcaaa tttaaaagaa
    15361 ttattacttg ataatataag tgttttaaag caatcaactg gaacttctcg agaaatggat
    15421 tttgtatcta gctcatataa tatcggttta tatataaaat tgtgttggat tgtagatgga
    15481 ggggaggagt ataaaatgaa aaactatatg gaaagtaatt taatatattc aagagtaggt
    15541 aaagacttat ttaataaaat gacagatgaa tccagaagaa aaaatttttc tatgaaagtg
    15601 attcaactac ttaagtctaa tgatagaact cagctatttc aaactataat gcatgtgtta
    15661 gtttctaatg gaatcttaat aggagaaggg tttgtagaag gtctaatgca gagttctgaa
    15721 ttagaattaa atacgaatgt aggtctattt attcaagaaa tcatgaaata aaaaaggggg
    15781 aaaatttaaa atgaatgaaa aaagagtaaa aggagtaaca ttaactatac tgacagaaag
    15841 cccagttcca ctttcatatg accaagggta tggaaattat acaccaataa aaaaagaaca
    15901 atatagagaa aaaatacatg caaaaactag tatagccaca ataacatatg atttaagaag
    15961 aatgctccac caagaatatg gttggaattt aagtaatatt gtttttggaa aaaaaggaaa
    16021 tatatatcca tctattaaga aaaatgtagg atgtgtagat gaaaatggat tagagactga
    16081 tgtatttggt tatttaatac cacttaaaga taaaggttct ataagtaaag cttctccact
    16141 tagaataata ccttttaggt cattaaatcc atataaaggt tcaacacagc taataacaaa
    16201 tagaggcttt ttaagtagtg aatttggtag aaaatattat gatgagaagg aagaaaatga
    16261 agtacctaga gatgaaaact ttccaacatc acaagcatta gcaatagaag agactttatc
    16321 agattactac gtttatacaa taacattgga gcttgataga ataggagtag tagaagtaga
    16381 agatggaaag ttacttttac cagaagaaag aaaatttatg agtaaggagt taagagaaaa
    16441 agcagtaaaa gatatattag atgcaattac tgtgtttaca agaaacataa aacattcatc
    16501 agtactatta aaaccacttg cagtaatagg tggagctttt gataaagttg taccattttt
    16561 ttgggatgat gtagattata atgctgatag tggtgagata aatttagagg gagtcataga
    16621 aactatagaa agttatagcc ttaaagaaag taacacaata cttgctatca atgatagatt
    16681 aaaaatttct aataaaaagg aattaaataa gtttaattta agtaaatacc ctgtaaaaga
    16741 gattaaaaat ctagcagata gattggaaat tggagaagat aatatgtggt atttaaaaga
    16801 gtaggaggtt ttttgaatta tgaaagtatt acaatgtaaa cttaagcaac caactgctca
    16861 ctatagagac ccaaaagtat ttcaaaatga atatataagt actttaaatt taccatctaa
    16921 gaccacaata atgggaatga taacgtattt atgtgataga agattaaact ctgatataga
    16981 tataggaata attggaacac atcatcatag agaactagaa ttttcaagag gagaaaacat
    17041 tgatttttgg aatgaatata taaaaatggg aaaaggtaaa gataaagaaa agtttttgct
    17101 taaaggaaac tactatgatt actataaaga gcataaagct caaaatagca tattaaacta
    17161 tgaagttctt aaagaagtag aactgactat atttatttct tgcaaggatg atgaagaact
    17221 tgaatttatt agaaaaaagc ttgaaagtcc atgtaaatat gcaaatttag gaagaaaaga
    17281 agattttgta ataccaagtg aaaaagggtg ttttgtgaaa gaagttaagt taaaagaggt
    17341 tatacctatg aatactcgtg atgctataaa agaaaatata aaattaaaga atacatatgt
    17401 aagagttgac ttaagagata aagagaatgt cgagactata ataaatcaag gtgtattgat
    17461 tgcattgcct cataaatata aagatttaga agcaaataga gatgatagag tttatgagtt
    17521 ttgccattat atatatgttg ataatgatgg tatatatcct aaaaatatta aggtaaatgt
    17581 atgtattgat acaaaagaag tttttacatg gctttaaatt attttgaaag ataggtgttt
    17641 atcatgaaaa atatcatgca aaaagaaata ttagctaagt catacaattt aagaactaat
    17701 caacaagaac aaacattagt tgaacatatt aaggatttat ttgaagtatt agaatctata
    17761 ttagaattaa atctatattc agataaagat gtagagatac taaaaatatg ttgtgctttg
    17821 cacgatttag gaaaaattaa ttcaattatg caacaaaaaa tggagattaa taataaaata
    17881 tcgtatagtt gctcagaaga agagaggaaa aagttagagt ctgacaaaaa atctttaaaa
    17941 aagatagcaa gacataatat attttctgga gcatttttaa aagatatatt agaaaaaatg
    18001 aacttgtcag aagaagataa gttatatata tataaaagta taatgttaca tcatggaaac
    18061 tatgaagatt atatgaggtt aagtattagt aaagttcaag aagaaattta tgagtatatt
    18121 gaaaaaggca ttttagaaaa agaagagttt aatctgaaag atatagaaag ttatataaat
    18181 agcattttaa atgtagactt taaatttggt gaagatgtat tggattatga ttacatagat
    18241 aaattaagtg aaagtatgtt tatagacagt gattataaca atggtcaaat agatgattca
    18301 gttttaaatt atagaaagtt taagtatatt ttatataaag ggatgctgaa cttaatagac
    18361 catagtgcat caagtagaca gaaaggtata aaattttata acgattttac tgatgaagaa
    18421 attgataata tgattttaaa tgagatatat aagagtcagg gtaatttgga aaaaaataat
    18481 atagagttca atactataca aaaaaggttg agagaacttt ctggaagaaa tgttctgact
    18541 gttgcattta caggttcagg aaaaacagct gcagattata gaaagacttt taaaagaaaa
    18601 ttctttttag tgccaaataa aatatcagca gaaagttttt atagaaaaaa tatttttcaa
    18661 aataaaaatg atttagatac aagaagtagt aatgattaca ttggattact tcatggagat
    18721 ataaacttat attcagagaa tgaagataat ggagaacatg attttgtatt gacattaagg
    18781 gatatagatt tgagtataaa cttttgcaaa ccttgtgtaa ttgcaacagt tgatcagtta
    18841 ttacttagta tgtttaaatt tccgggatat gaaaaaatat tcgcagccgt aaaaaatgca
    18901 tctataactg ttgatgaagt acatttattg gaaccaaaga tgtttttgat tttgatatat
    18961 tttatgcagt ttgcatgcaa gtacttagat gttgattttc acttaatgac agctacactt
    19021 ccaaaatcat ataaagagca aatgataaac aagggaatta tttttcaaga agaaagtaat
    19081 gaaaatgtta ctgataaagg agaaatagtt tttattgaga gcaacaagga agaagagatt
    19141 tgtgaaggta aagatgtaaa ggtttctttt ataaaggaaa aagaaatcaa gtcaatagtt
    19201 gaagatgctt tagaaaataa acaaaaaatt cttataataa agaatactat tgattcagtc
    19261 aatagaacat atgagttttt aaaagaaaat ttatctgata agtatggtgg tgtagatata
    19321 gatgttttac atagtagatt taagttcaaa gataaaaaag agaaatatag taaaatatta
    19381 aatggagagg gtgatatttg gatatcaact caatcagtag aaatatcctt ggatttggat
    19441 ttcaatataa taataagtga tttagcaact atggatagct taatacaaag aatgggtaga
    19501 tgcaatagaa ataataagta tgaatatgga aacttttata ttctacctag tgaagataaa
    19561 atttatgaca acaaacttaa gagcacaact aaaaacatct taaaggacat attaaaaact
    19621 aactctattt ttacaatggg aattagaaaa actattttgg atgattacta tgataatagt
    19681 gtagtcaaaa aatattttga agagaatttt ataagttgtg ataaagaaat taagaatatt
    19741 tatggtgtaa ataaagaaat ttttgatgga ttggatttaa tttttaattt tgaaccttac
    19801 aaaaatattg ttgatagtaa gaaggaagca gctaaaattt ttagagatgt tgatgttagt
    19861 tacaaaatta ttctggaaga agatttttat aaggaagata gagagttaca acaagattcg
    19921 attcaagtat cagggtttat atttaataga ttatattatt atgggttaat aagcaaagta
    19981 gaaggataca tggtactaaa atctagtagt aagtttgagt ataatagtac tgtaggctta
    20041 attttgaagt gagcatatgt ttaaatataa tataccttta gacattgaaa atacaaaaag
    20101 tactactgta agctcactgt aaaaactatt ttttaaaagg aaaaataagt tgtgagatag
    20161 agtgtttaca agtgtatata gataataaat ttgaggttta agattaacta agtgggtttc
    20221 aggtacatcg cgaaaataat tataagttgg agattttgta tgtttaagat taactaagtg
    20281 gatttcaaat attgatatag tagatataat agaagtttta ttattaggtt taaaattaac
    20341 taatcggatt tttaaatatt ctgactatta tataaggata ttattagatt gaatttaaaa
    20401 ccaataatat aaaacaacaa aatattatat ttatgatata ataaaagtta tggaaaaaca
    20461 ggaatattac tatagagaag agaggtaaac ttatgaataa ttggatagaa gtaactataa
    20521 aaacaacaac agaagcagtt gaacctataa cgaatatact atatgaacaa ggagctggag
    20581 gagctgtaat agaagaccca aaagactttt tattccaaaa gaaaaatgaa ctagactggg
    20641 attatgttga agaagaagta tttaaaaaga atgaagaaga tgatgttctt ataaaaactt
    20701 atgtttctga agaaaaaaat gtgatggaat ttgtcgaaat aataaagcaa aaagttttag
    20761 gattaaaaga ttttggtata gatataggtg aaggaagtgt atcattggac caagtaaatg
    20821 aagctgattg ggcaaatgca tggaaagcat actacaaacc aacaaaagta ggacaaaggg
    20881 tagtagtaaa gcctacatgg gaagattatg ctatgcaaga gggggattta atcatagagc
    20941 ttgaccctgg aatggcattt ggtacaggta cacatgagac tactagtatg tgcattagag
    21001 aattagaaaa gtatgtgaat aaagattcta aagtatttga tattggatgt ggtagtggaa
    21061 tccttgcaat agcagcagct aaattaggag caaaagaagt tgtagctgta gatttagatg
    21121 aagtggctgt aaaagttgca aaagaaaatg ttcttgaaaa taaagttgaa aaaagtgtat
    21181 ctgtaatgca tggtaattta actgatgtaa taaaagataa agcggatgta atagttgcaa
    21241 atataatagc tgatataata aagatacttg caaaagatgt tcaaaacttt atgaaagaag
    21301 atgctatatt catatcttca ggtataattt tggataaagt agaagaagtt aaagaaagct
    21361 taattgaaaa tggatttgaa atagttgaag ttcaaaaatt aggtgaatgg tcagcaatag
    21421 tttctaagct taaaaaataa gggatatggt gacgtataat ggatagattt tttgttgaaa
    21481 aaaataatat aaatctacaa gataaaactt gcactataga aggtgaagat gtaaaacata
    21541 tttcaaaagt tttaagatgc aaattaggtg agaaacttga aatatgtgac aagaataata
    21601 atgagtatat ttgtgagata atgaatatag ataagagtat agttaattta gaaattttag
    21661 aaaaagttga tataaataga gaatcagaac ttaaagtaag gctataccag ggacttccaa
    21721 aagctccaaa aatggaaata atacttcaaa aattaactga agtgggtgta gaagaaataa
    21781 tattagttca aactaagaga agtgttgtta aagttgatga caaaaaagaa gataaaaaat
    21841 ttgaacgttg ggaaagaata atttatgaag cagcaaagca aagcaaaaga ggtaagatac
    21901 caaaattaag aggtgtttta agttttaaag aagctttaga agatatgaaa aaaaatagtg
    21961 taaatatttg cccttatgaa aatgaaagaa cagtttcaat aaaacatgca ctaaaaaaat
    22021 gtgattctaa tatagatagt gttggaattt ttataggtcc agaaggtgga ttttctgaag
    22081 aagaaataga acaaatacaa aaaaataatt gtaatgttgt atcattgggg cctagaatat
    22141 taagaactga gacagcttct gttgtagcat caacaatagc tttgtatgaa ctaagtgatt
    22201 taggaggaga aaaataaatt gaaaaaagta gctttttata cacttgggtg taaagttaat
    22261 caatatgaaa cagaagctat gctagaacta tttgagaaag atggttatga acaagtcaat
    22321 agtgaagagt atgctgatgt ttatgttata aatacatgta cagttactca tatgagtgat
    22381 agaaaatctc gtcaatatat aagacgagtt aaaaagaaga atccagatgc aataatagcc
    22441 gttgtaggat gttattctca ggtatctccg gaagaaattt tggatataga agaagtaaat
    22501 ttagtcatgg gtacaaatga tagaagaaag attgttgaag aaattaaaaa gataaattca
    22561 agtaagaaag ttagtacagt tgatgacata atgaaagtta aagcttttga agaaatagaa
    22621 atcagccaaa ctaatggaaa gactagagca tttatgaaaa tacaagatgg atgtgacaga
    22681 ttttgtactt attgtatcat tccttatgcc cgaggacgcg ttagaagtag agatattgat
    22741 agtatagtcg atgaagttaa aaaattggct aataatggct ataaagaggt tgtattaaca
    22801 ggaatacatg ttgcctctta tggaaaagat ttaaaggata gggatattaa acttcttgat
    22861 gtaataaaac aaataaacca gatagaaaaa atagaaagaa ttaggcttag ttctgttgaa
    22921 ccaattttat ttacagatga atttgtaaat gaagttttaa agatggataa agtttgtcct
    22981 cattaccatt tatcacttca aagtggatgt gatgaaactc taaaaagaat gaatagacga
    23041 tacacaactt tagagtacaa aactattgta gatagattga gaagtaaaat gccagatgtg
    23101 gcaataacta cagatgtaat agtaggtttt cctggagaga caaatgaaga gtttaaaaaa
    23161 acgtacgagt ttttaaaaga aatagagctt tcacaaatgc atatttttaa atattcacca
    23221 agaaaaggaa ctccagctgc aactatggaa aatcaagttg accctcaaat gaaacacttt
    23281 agaagtgaac aacttttaaa tttaagtaaa gtaaatttta ataaatttgc aactaaattt
    23341 atcggccgtg agttggatgt tttatttgaa caaaatatag aaggcaataa atatgaggga
    23401 ttaacttcaa attatataag agttgttgtt gaaagtgaca aaaatataca aggtcagata
    23461 ttaaaagtga aaataaatga tgtaaaagat gaatatgtag aaggaatttt gctataacat
    23521 gtagaaatta taatatttat aagataggag gtggaaagaa tggattgtat tttttgtaaa
    23581 atagcgaacg gagaaatacc ttcaactaaa gtttatgaag atgatagagt cttagctttt
    23641 aatgacttga acccagttgc tccatatcat atattagttg tacctaaaaa acattatgat
    23701 agtttaattg atattcctga taaagagatg gacattgtgt cacatattca tgttgttatt
    23761 aataaaatag ctaaagagaa aggttttgac caaactggat tcagagtcat aaataattgt
    23821 ggaagtgatg gagggcaaga agttaaacat ttgcactatc atattttagc tggtaaaaag
    23881 cttcctaatt acgaagcggg acaaaactaa actaaattat tgaaaaattg agttccttct
    23941 tgataaaaat acttgataaa agtaaaaatt tagtttataa taaacaaagt gttaggttgt
    24001 aatgcatatt atgcaaaaag aacatagtta cagtcccagc ataattgcgc tattggaggg
    24061 agggaaaaag aaatgtcaga agtaagagtt agagagaatg aaacattaga cagcgcttta
    24121 agaagattca agcgtcaatg tgcaatgtct ggcatcatgt cagaagtgag aaaaagagag
    24181 cactatgata agccaagcgt taagcgtaag aaaaaagctg aagcagcaag aagaaaaaac
    24241 gctaagaaat agtagtaaat aagaataaag aggtgaaggg gatgtccctt aagcaaaagt
    24301 tacaagagga tttgaaatct tcgatgaaaa acaaagatac agtgagaaaa tctgtagtaa
    24361 ctttaataag agcttctata aagcagtatg aggttgataa tagggtcgag cttgatgaag
    24421 atggaattat cgatgtaata gcaaaacaat taaagcaacg tagagatgca ttagttgaat
    24481 ttgaaaaagc tggaagagaa gacttaataa aagaaacaga aggtgaaatt gaagttttaa
    24541 aagagtacct acctcaacaa ttaagcgaag aagagttaga agaaattgta aaatctacta
    24601 tatccgaagt aggggcaact tctatgaagg atatgggaaa aataatgtca gttatccaac
    24661 caaaagttaa aggaagagct gacggaaaac ttataaataa gttggtaaaa caaaacttac
    24721 aatagattga tatagcctag agagaaatct ctaggctttt tatagttttt gggaataacg
    24781 agtcatattt gagcatatct ataatcaaag aaaattttga aagaagggga aaaatttgat
    24841 tatatcagta aaagagatta ggaatttttt gataaacata tttataccac taataatagg
    24901 ttacttaagt agcatcttaa caatgattat cagtggaaca gatatttcaa cctattattt
    24961 acaactcaca aagccaagct ttgcaccacc agcttttatc tttcctatag tttggactat
    25021 attgtacata ttgatgggta tatcttcata cctaatatta agaaagggtt acaacttacc
    25081 taaagtaaga gatgcaattt tttattatgg attacaactt gtattaaact ttatttggag
    25141 tatactattt tttggatttg gacttagatt tacagctttg atagatataa taataatgat
    25201 tttggtttct ataataatga tttcgaagtt ttctaaaata gataagagat ctggatacat
    25261 aaatttgatt tatttaatgt ggcttgttta tgcaggattt ttgaactatt ttatatggtt
    25321 tattaataaa tagaatgtaa agattttgta aattaccctg tacttttaat aatatcataa
    25381 caaacgaaaa ataaatagac tattaaaaat aatcattttc atatacaata atattaattt
    25441 aaatacaata aatttataaa aattgtattt aaagattaac atattaaagt attagaattg
    25501 gaaaggcttg aagggagagg tttttatgca aataatagta tctgggagac aaatgaaatt
    25561 aactgacgga ataaaaggat acgtagatgg aaaattaagt aggttagaaa aataccttga
    25621 tcctgaatca gaagtgaaag taacagtaag tgcgaaaaag gatagacaaa aagtggaggt
    25681 tacaataatt cctattaacg gtcaaataat aagagcagaa gatgtagaag atgacttata
    25741 tgcagcaatt gatattgttt gtgacaaatt aagtagacaa gttgtcaaat ataaaactaa
    25801 agttaaagac aaagttcaaa ataataagag cataagattt gaaaatcttg actttataga
    25861 taatagcagt gaatttgatg attatgatta tgatgatgaa gaagatgaaa atatagtaat
    25921 agaaagaaga aagaaattta atgtaaaacc tatgagttct gaggaggcga tacttcaaat
    25981 ggaactagta ggacacaatt tctatatgtt tagaaaccaa gataattttg aaataaacat
    26041 agtgtataag cgtaaagctg gtggatatgg tttaatagaa caagattaat aaaaattgta
    26101 tacgtttaga ttttatataa gaaaaaataa aaaggtaatc atgttgattt tttatcaata
    26161 tgattacctt tttcttgttt aaataatata ttagatatta ggtatttatt atataaaaat
    26221 tttaaaacta atctgttaca acatcaataa agtgcataaa tacctatttc aagcataaaa
    26281 ttaattaagg gggtaatgct tatgttagaa atatccactg atttacaagt aaatcaacca
    26341 gttattactg ttacaagtaa tacgtttatt agtatagaaa attatctatc aatattagaa
    26401 tatgaagttg atttaataag aataaagact aaagttaaaa caattaaaat atctggggat
    26461 aaactttcac ttaagtatat tactgatagt gagataggta taaaagggat tatctacaat
    26521 gttgagtatg ttgattagga ggagatacac ttgataagtt ttataagggg atactatgtt
    26581 atagttgtgg aaggagtagg actcgaacaa tttttaaatc atcttataag aaatgggata
    26641 agtgtttaca atgtaactag aataaaaaat actaaaatgg agtttcatat agatagacaa
    26701 gatataaaag actttaagaa tgtttataga ggaagtaagt ttgatataaa ggtaaagcaa
    26761 aaaactgggg tcccttttat tatcaaaaga gtttataagc acaaaggtat gtggatttgt
    26821 gctcttgttt ctttattttt attgatgtct acatcacagt ttgtgacaga tgtatatata
    26881 caatctcctg aaggtataaa aaaagaagct cttagaaatg agctttataa ggttggagta
    26941 aggccaggag tctataagaa gagtattgat agaaaagaag ttagagacca tatgatgtca
    27001 aaatttaatg atgtggcata tttatctatc aatgtaaaag gaacaaatat atttgttact
    27061 gttacaaaaa aggcagaatc cctaaaatct acagaccaat caaattattg taatgtaatt
    27121 gctttaaaaa atggtataat agaaaaggta atccctagaa gtggtaaatc agttattaaa
    27181 tctggagaca tagtccaaaa gggagatgtc ttattaaatg gagctaatac caagtcaata
    27241 ccagaagtat gggcaagtac tttttatgag tccacaaaaa aggcaagtta tgtagatact
    27301 gtaaataaaa aaactggaga aaagaaaaat atatatacac taagttttta tgataaagag
    27361 tttacaatga gaaaaaatat aaaatataaa gattatgttg ttgaaaataa agaaaaaaaa
    27421 ctatcaatag gaaactatac ttttccaata aagataaaaa cgagtacttt ttatgaaaca
    27481 aaaaaggttg aagtaaaaag gaataaagaa gagttaaaaa aagaattatc tgaaaaagct
    27541 ttaaaagagt tagaatacat aatcccagct tcggctagaa taatagatgt aaaacataac
    27601 tttaaggtta ataaaaatat gttagaatat ttaataacag ttcaaactag tgaaaatatt
    27661 gctaaaattt atcctcttag taagtcagaa gcagaaaggt ttataaagga agaaagtaaa
    27721 ccagatgaag gtgaagagga agttccttcc aatccagaaa aaagaccatt aaatgatata
    27781 agaaatgagt ttgatgagga taataaggat aataaagacc aaaacaattc tgatgaaaat
    27841 aatagtaatc aaaatagtaa taatagtcaa aataataata gcaataacaa ctaataaaat
    27901 taaacttaaa atcttaggag gactaattca attgataata caaaagaaat ttacaatagc
    27961 agatggtaac tttgaaagag agttatttgg caactttgat gaaaatgtaa aattaataga
    28021 gaagacttta aatatagatg taatacttag agaaggcaat ataattttaa ttggagaaga
    28081 aaaaaatgta gattctgcat taaaacttat gaatgaatta caccaaactg tatcaaatgg
    28141 taagcattta gataagcaga gtatttctta ttcattatct cttttgcttg aaggaagtga
    28201 acaaaaaata aaagaacttg aggggacaat cgtcataact caaaggggaa aagctgttca
    28261 acctaaaact ttaggtcaaa aagaatatat aaaacttata gaaaataatg atataacttt
    28321 tggtgttgga cctgctggaa caggaaagac ttatcttgcg gttgctatgg cagtaaaagc
    28381 ttttaaaaga gatgaagtta gtagaattat acttacaaga ccagctgtgg aagcaggaga
    28441 gagtctaggt tttttaccag gagatttaaa ggacaaagtt gacccatatt taagaccact
    28501 atatgatgcc ttatttgaaa tgttgggtgc tgataaattt aataagtatt tagaaagagg
    28561 tactattgag gttgcaccac ttgcatttat gagaggtaga acacttgata attcatttat
    28621 aatattggat gaagctcaaa atactacttc tgaacaaatg aaaatgtttt taactagact
    28681 tggatttggc tctaaagctg tagttacagg agatgtaact caaacagatt taccacaaaa
    28741 taagaagagt ggtctaatac aagctacaga gattcttaaa ggtgttccag gtattgggtc
    28801 tataatgcta acagatagag atgttgtaag acatgagttg gtacagagaa taataagagc
    28861 ttatgagaag cacgacaaaa gagaagaatt taaaaaagaa gaacgtaaaa aagcgaaaat
    28921 gcaggaaaag actttcaaaa gaaagtaaaa gtgtagtaat atagatatat agcattatta
    28981 atgaataaaa agtaggtgta gtgaatggat ttgatactag atgatagaca agataaatta
    29041 gaagtaagtg aggagttaat tgaaaagata aaggatataa tcatagaatg tttagattat
    29101 gaaggatatg atgataatta tgaagtgagt ctttcttttg tagataataa agagattcat
    29161 gaattaaata gagaatatag aggagttgat agagtgactg atgttttatc atttcctctt
    29221 ttaagtgatg attttgaaga tgttgagtta gaagaagaat ccctaggtga catagtagta
    29281 tctcttgaaa gagccttaga acagagtata gagtataacc atagttttga aagagaagta
    29341 tgttttttaa tatgtcatag tatgtttcat cttttaggtt atgaccacga tacagatgaa
    29401 aatacaaaag aaatgagaga aaaagaagaa cacatattaa ataagttaaa cataacaagg
    29461 gagtagtttt tatgaaacca gaaaaaactc gtcaaggtat aattaaagca tttaatgcag
    29521 ctatagaagg gatattgtat acatttaaat ttgaaagaaa tatgaaaata cattatttag
    29581 gttcagttgc tgttcttata ataagtttgt tttttaactt ttcaaaatta gaaatgataa
    29641 tgttacttat gtccatatgt ttagttgtag ttgctgaaat gtttaataca gctatagaaa
    29701 aagctgttga tttagtaact gatgaatatc atgttcttgc taagatagca aaagatgttg
    29761 cagctggagg cgttctagtg gcagcactaa attcagtagt agttggatat attttgtttt
    29821 atgataaatt gacagatata tctggaatat taatttataa aataagagaa tcagaattac
    29881 atataacctt gatatgtata ttattggttc ttatagctgt tgtagttgta aaagcattga
    29941 cttctacagg tactcctcta aaaggaggaa tgccaagtgg tcatgctgcc ttagcatttg
    30001 ctatagcaac tgcaattaca cttatgactg aaagagttgt tgcctctact cttgcatata
    30061 taatggctgt attagttgcc caaagtagaa ttgaaggaaa aatacataca ttttgggaga
    30121 ctattgctgg agctttgtta ggtgtgttga tagctatatt agtatttcag cttggaatgt
    30181 tctataatta aaataaatgt acataaaata tttaaaataa aacaaatctg tgctaattaa
    30241 atttttatga tataatgtag agtattaaat aaaaataact tttaattatt gtatacagga
    30301 ggagttgttg tggataacat agaattgtta aggttggcag aagatgccag acaacattct
    30361 tatgctcctt actcaggttt tagagtaggt gcagctcttt taactaagag tggtaaagtt
    30421 tatactggat gtaatataga atgtgcatca ttaggtggaa caaattgtgc agaaagaaca
    30481 gctattttta aagcaatatc agagggtgat aaggatatct ataaaatagc tatagcaagt
    30541 gataactcag aaaataatga gcaaacatat ccatgtggaa tatgcaggca ggtaattata
    30601 gagtttggtt ctgatataaa aataataacg ggatatacta aaggagaggt gtttgagcat
    30661 agtataaagg atttgttgcc aaactatttc tctggaaatg atttaaaata aatttaaaag
    30721 tataaataaa tttaagtaat taaaacaaga agataaaaag ttaggagaca gttaatgttc
    30781 aaatcaggtt ttgtcagtat agtgggtaga ccaaatgtag gaaaatctac tttaatgaat
    30841 aatgtagttg gagaaaaaat agctataatg agtgataagc ctcaaactac aagaaataca
    30901 atacaagcag tatatacaga tgaagaaatg cagatagtat tcttagatac accaggtatt
    30961 cacaaaccaa aaaataaatt aggtgaattt atggtaaagg cagcaacaga agcttttaaa
    31021 aatgtagatt taatattatt tgtagttgat gattctaaaa aaataggtcc tggagataga
    31081 aagataatag aggatttaag aagtgttaag acaccaataa ttttagttgt aaacaaaata
    31141 gaccaactag accaaaaaga tgagctattt gatataatca aaatgtatga tagagaaggt
    31201 atttttaaag aaatagtacc aatatcagca ttaaaaggaa agaatacgga taccttaata
    31261 aaagttatac aaaattattt agaagaaggg ccaaagtatt ttccagatta tatgattaca
    31321 gaccagccag aaagagtatt aatagcagaa cttataagag aaaaagttct tcactatctt
    31381 aatgatgaaa tacctcatgg tgtagctgtt gaaattgaaa aaatgaaagc gagaaatgat
    31441 aaggaaatag tagatgtatc tgctgtaata tactgtgaaa gagattcaca taagggaata
    31501 ataataggaa aaaatggtag gaaactaaaa ggaataggta agtcagcaag acaagatata
    31561 gaattgcttt taggttcaca aataaatctt caattatggg ttaaagtcaa ggaaaattgg
    31621 agaaatttac aaaattatat aaataatttt ggatataatg ataaataaag gtgggttata
    31681 tggataggaa actggatgct tcccaagatt tattatatga agtaaaatca ttaattgata
    31741 ataataaagt attggagtta agagaattaa tagaggaata tcatataatt gatatattcg
    31801 atataatgga aaatttagag gaagacatga aaattcaatt atttgaagta cttcctttag
    31861 atatggcatc ttctatatta gaagaaggta gtgtagagtt ttttattagt attttatcta
    31921 aactggatgt agaacactcg aaaaatatac ttgaacttat gtctcttgga gatatggcag
    31981 acaagttgag tgaacttgaa gaagaagaaa gagaacacat aataaatctt ttgaatcaag
    32041 agaatgccga ttatgtaaaa gagttactat tctatgatga agattctgct ggtggaacaa
    32101 tgaccactgg atatatttct ataaataaag atatgacagc tcttgaggct atagaccata
    32161 tgagagaaga agctgaagaa gctgaaacta tttattatat atatgtggtt gatgatgaag
    32221 aaaaactagt tggagtattg tcattaagag aattaataat agctagagac gcaaatatag
    32281 tagaagattt gatgagtgaa aatataatct ctgtttatgt agatgaagat agagaagaag
    32341 cagtaagatt agtatctaaa tacaatttaa tagcaattcc agtagtagac agacaagaaa
    32401 aattaaaagg aataataaca gtagatgata taattgatgt aatggaagaa gaagcaactg
    32461 aagatatgta taaatttgca ggaagttcag aacatgaaag agaagttgcg gaaaaagaaa
    32521 accctacatt gagagagcaa ataatatcag cacttagagg acgattacct tggttaatta
    32581 ttaccttagt tggagggttg ttggcttcgt taatactttc taacttagac tatataatga
    32641 atcctgtata tgctccatta gtatttttta tacctgtagt aattggtatg ggtggaaata
    32701 ttggaactca atcatcatct gttacagtaa taacattgtc taataaggat ttaaatttta
    32761 gcaatgtggt tagagaaggt attgtaggta ttattacagg tcttttgtgt agtataataa
    32821 caggtattgt aatttacttt gttatgagaa agttagacat tgtattaatt gtttcaatat
    32881 cattatttat aaatatggtt ttaggtgcaa caataggtgc gtttatgcct gttttattaa
    32941 aaaaaatgga tgcagaccca tctacagttt catcaccaat tatatctaca gctcttgata
    33001 taactggtat agcagtttac tttataataa caacggcatt attgtcaaaa attgtttaat
    33061 aactttatgt atgatttatc ctcttcttac taaaaatata agtataaatg taagaagggg
    33121 gttttttaaa aatgaacaat atcagttggg aagtttttaa aaaaacaggt agtatagatg
    33181 catatttata tttctgtgat tgcaaaaaca tacatgaaga aattgaagaa gtaagggaga
    33241 aaaaagaaga cgatgataat cctgaacacc caggggatag tacttaaagc tataaggtat
    33301 aaggaaagtg atataatact tacactattt acaagaaagc ttggaaaagt atctgcaata
    33361 gcaaaaggag ctaaaaaaaa taagagctct ttgctttcat catcacagtt attttcttat
    33421 agcaatttta ccttaaaaaa acaaggtaat atgtacaagg ttactcaaag tgaaataata
    33481 aaaagttttt ataatatatc ctatgatata gaggcatttt cttatgctac atatattaca
    33541 aaattagttg aaaattctat acttgaaaat cagactaata ataggctatt tatattactt
    33601 gcgcagactc tgtatttgta tacacaggat aatacagaca ataggtttat aacagcagca
    33661 ttcgagttaa aatttttaga ctatatagga tttaaaccta tagtaaataa atgcattaat
    33721 tgtgaatgta aaattttaca aaattctgtg tttaatatct acgaaggtgg tatactatgt
    33781 agtaagtgta gtaaactttt tgataataat ataaaattag acttaacaac tataagtctt
    33841 atggaatatg tattaagaaa tgatatttta acatgtagta aagctaaagt atcaaaatat
    33901 ataacacatg aacttgagaa tattctagac aagtatttga aagtttatgt tgataatatt
    33961 aattttaaat cattacacgt attgcaaagt gtaaaaaata ataagggagt ggataatgat
    34021 gagtagtaat ataactattg aaatggttga ttcagtcttt gaaagagttc caggagcaac
    34081 ttatgcagaa gcaaaagaag cattagtaat gtgcaatgga gatgtaattg aggcagtaat
    34141 atatttagaa aataaaaaaa atacttgtaa ttgtaaagca aaatctgcta aagaaactat
    34201 ggaagaagta tttggcaaag atggagaaaa tataaagttt caattaaaag agcttcttag
    34261 aaaaagtagt gtagtgcgaa taatagttga aaaagatgga aaaactatga tgaacattcc
    34321 tttaacagtt ggagttgttg gattagcttt tgtaccatta gctactttag ttggattatc
    34381 agctgctgtt ataagtaaat atcgtataaa agttcaaaat gaagaagatg gagaaattgt
    34441 agatttaggt gaattgaatg aagaaaaact tcacgttttg aagaatatga taactaatgc
    34501 agcaaaagat gtgaaggatg tagtagttga caaaaaagaa gatgacaaag atgtaactgc
    34561 tgaccttatg aaagaagaat ctgaaaagaa taaagaagac gaagacgaaa ataaatagat
    34621 ataaagtgac ttttaaaaaa tgaagagaag ataattataa gaattatcac taacattgac
    34681 aaattttata gaatttgata ttctaaaagt tgtagaagaa ctattaaaat agcctttcgt
    34741 gtagacgaga ggctttattg ttataggagg gatttttatg aattttcaag agatgatact
    34801 ggcgttgcaa aaatactggt caaagcaagg ttgtatcatg atgcagcctt atgatattga
    34861 aaaaggtgca ggaactatga atcctaatac ttttttaaga tcattaggac ctgagccttg
    34921 gcaagtatgc tatgtagagc cttcaagaag accagcagat ggaagatatg gtgaaaaccc
    34981 aaacagacta tatcaacatc atcaattcca agttatacta aaaccttctc cagataatat
    35041 acaagaatta tatttggaaa gtttaaaaga gattggaatt gacccaagtg agcatgacat
    35101 aagatttgtt gaagataact gggaagctgc tacagttggt gcttggggac ttggatggga
    35161 agtttggcta gatggtatgg aaataactca atttacgtat tttcaacaag taggtaatat
    35221 tgaatgtgag cttgagacag gagaaattac atatggatta gaaagattag ctatgtacat
    35281 tcaagaagtt gatagtgttt atgatttaaa atggaatgat aaaataactt atggtgaagt
    35341 atttaataaa gctgagtatg aaaactcaat gtacgcattt gaattatgtg atgcagatat
    35401 gttgtttaat ctgtttgata tatatgaaaa agaagcatta agacttatgg aaaatggcct
    35461 tgtaatacct tcatatgact atgtgttaaa atgctctcat gcatttaata cattggatgc
    35521 aagaggtgca ataggagtta gtcaaagagc ttcatttata ggtagagtta gaaatatggc
    35581 aaagactgtt gcagaaactt ttgtaaaaca aagagaagaa atgggattcc ctcttttaaa
    35641 ggacggtgac aaataatgaa aaattatctt ttatttgaaa tcggtgttga agaaatgcca
    35701 tcaagatttg taggcagtac actagaacaa ttaaaaaaca atcttagtaa attatttgat
    35761 gaaaatagaa tagcttttga taaaataaat gcgtatggga caccaagaag acttacttta
    35821 gtagtcgaag gattgagtga tagacaaaat gatttagaag aggaaataaa gggtcctgct
    35881 aagaaaattg ctgttgatgc agaaaataat ttaacaaaac cagcattagg atttataaaa
    35941 agcaaaggat taagcgaatc tgacatatat tttaaacaag ttggaaaaga agaatatata
    36001 tttgcgacta taaagcaaga tggaaaagaa acttcagaag tactaaaaga catattacca
    36061 gagactataa aatctgtagt attcccaaaa gctatgcgtt ggggaggaaa aaatcttaaa
    36121 tttgcaagac ctataagatg gatagttgca ttgttaaatg ataaagtatt agagtttgat
    36181 ttagaaggaa taatatcttc taatgtaaca aaaggtcata gatttttagg tgaaagtaca
    36241 atagaagttg attctataga agattatttt gaaaaattag aaaaaaatta tatagtttta
    36301 gaccaaaaca agagaaaaga aatgataaga aaacaatgta tagaagtggc aaattcttta
    36361 ggtggagaag ttgaatttga tgaagattta cttgaagagg taacttactt agttgaatat
    36421 ccaactgctt tttatggaga atttgatgtg gattatataa aactaccaaa agaagttgtt
    36481 ataactccta tgaaacaaca tcaaagatat tttccagtac ttaaagaagg aaaactgtta
    36541 cctaacttta tagctgttag aaatggtgat tcttacagaa tagacaatgt taaagctgga
    36601 aatgaaaaag ttttagaagc aagacttgca gatgctttat ttttctatag agaagatact
    36661 aagagaaact tagaaagcta catagaaaaa ttaaaaactg ttgtattcca agtaaaacta
    36721 ggaactattt acgacaaaac tttaagactt gaaaaattaa gtgctgatat acttgatagt
    36781 ttaggtttag tagatgaaaa ggcagatact ataagggctg ctaaattatc taaggcagat
    36841 ttagttactg gaatggtagg agaatttgat gaattacaag gttttatggg aaaagaatac
    36901 gcaaaagttg gaggagaaaa tgatgttgta agtgaagcta tttttgaaca ttatcttcca
    36961 agatttgcag gagatatact tcctaagact aatgcaggag ttgccttatc aatagctgat
    37021 aagttggatt caatagcagg tttctttgct ataggaatac aaccaacagg ttctcaagac
    37081 ccatatgctt taagaagaca agctttaggt atacttagta tacttatgga cagaaaagtt
    37141 gctgtggata taaaagtact tattgaacat gctttagaca attattctga attagatttt
    37201 aataaagaag aagttgttga gcaaataatg aatttcttca atgagagagt aaaaaatcta
    37261 tttaaagatt taggaataag atatgatgtt gttgatgctg ttttaagttc tgatataaat
    37321 gatgtttctg atatgcatat gagagcttta gagcttaata attggctaga aaaagatgaa
    37381 ttagtggaga tgcttactgc atttaataga gtttctactc tagctcaaaa agcagaaagt
    37441 gataaaattg atgaatcttt attaaaagaa gatgctgaaa ttaaacttta caatgaattt
    37501 aaacaagtaa aggtaaaagt taatgaatta ttaaaggata agaaatatgg tattgcttta
    37561 gatgaatttg catctttaag accagtagtt gataatttat ttgatacagt tatggttatg
    37621 gataatgatg aagctattaa gaacaataga cttgctttac ttaaacaagt atatgatact
    37681 atgctaggaa tttgtgattt atctaaaatt gtttataaat aaaatttaaa ctatttataa
    37741 gaaataaggc tgtctcaaaa tagagatagt cttatttttc attgttgaga tgatgaaaat
    37801 atatttgatt tcatagaaaa agagtgtctc ataaactaaa aagttcattt tgagacaccc
    37861 atgctttttg taagtaaata taataaatta tattttatac ttatcagtta ttttgtttaa
    37921 aatattatca tttgaattta cccattcata aaaattgcct gatgatgcgt ccgatatttt
    37981 ataaagcatt tcaggaatat cactagcttt aaaatctcca agtgattttc ccaatctagt
    38041 cttacctatt tcaaaactgc ctccaacaaa taattcaaat gcatctgtag atataccatc
    38101 aacattcttc tttttaccac ataaacctat gctaccaact tgatgaacac cacatgaatt
    38161 taaacatcct gatatatata acttaggtgc tttatttaaa atttctttat tattttgtaa
    38221 tctaaaataa tctatgattt catgtaacat tttctgacta ttttgtatgc ccatttgaca
    38281 aattggaaca cctatgcaag atatactttg ttctaattga gaattacaac taatagtttt
    38341 acttatttct aatattcttt ttgcttcatt tccatttaaa tttaaaatat ataatccctc
    38401 tgtcattcct agtctaatca taggattttc aatattatct aattctttaa gtaaagtact
    38461 taaatccttt gttaacaaaa tacctccaac tggatgtata tatacagtgt ataaaccatc
    38521 ctgtttttga ggtattagtc tagaatcctg tatatctatt ttaaccccag gtttagagta
    38581 atctatttct tcaatattga gttcaagact accattttct ttttctaatt tatagtattc
    38641 tttaaatttt tctaagaata atttttcacc aagtgattcg accatatatc taaccctagc
    38701 tctgttttta ttttcataat ttccataatc cataaagaat ttaatcatac cttcaacaca
    38761 atataaggca tctttcggtt ttataagttc atcaagttct aaagcaactt tgggttgttt
    38821 acctagacct ccacctaaga aaactctgaa atatggttca tcatttttca aagtagcaac
    38881 aaatcctaag tcttgtatac tacaatgagc agtatctgtg taacaattag aataagaaac
    38941 ttttaatttt ctaggtaaat ggtatgtagt agcatttttg ataaaatatt tatcagtagc
    39001 aactgcatat ggagtaacat caaagacttc actagggtca actccagata atggagataa
    39061 tccgacattt ctaggataat taccaccacc acctctagtg aatatatcat tttttattcc
    39121 ttcttccatg atatcaacaa ttgaatttat agataaatcg tgtaattgta tagcttgtct
    39181 agttgttaag tgtatcttat ctaagttgtg tttaatagct atttcataga ttttattaag
    39241 ttgatactga gataaaactc cagcagatac tctaagtctt atcataaaag attttttgtc
    39301 tctttgagca taaacaccat atccaccaga aaatccctta tattgcattt tatttatttc
    39361 tccatttaaa aattttgaag aatattcttt aaaattaggt agttcattta acaatatctt
    39421 tttaaaatta tccattattt cacctcattg ttagtcttgt ataaaaatat atctattgta
    39481 taatagatat atatattatt atctagtcag gataaaaata taacagaata attctattaa
    39541 aagtaagaat aataaggaca tagtgagtat tttgttttta ttttacaaat taagtataat
    39601 atttttgttt tataaaatat aattaagata aatagataat tttgtaattt taatatataa
    39661 tatttagtag agtgtgggag tagcacttaa tatttgaagg aggagttttt gtatatggga
    39721 gaaataataa atgcattaga tagagcccta gacataattt tgctgttata tcatgaaaag
    39781 agagaaatgg gaataactga aataagcaag gctatgggag tatataaaag tactgtacat
    39841 agaactttgg ttactcttga aaacaaaggt tttgttatac aaaatgcaga gaatggtaag
    39901 tattggctag gaataaattt atatgcaata ggtatggttg tgggagaaaa aatgtcattg
    39961 actgaaatag taaaaccata tacgaaaaaa ttaaatcaag aatttaacga agtagttaat
    40021 gtatctatat tagaagaaag agctcaagat tctccaagaa gtatcataat acataaagag
    40081 tatggttcaa atcaattact ttcagtaaat ccatccgttg gttcaagtag tgaatgttat
    40141 tgttcagcag ttggtaaatg tcttatggca tttaatgatt ctatagattt tgaaaaatat
    40201 agaaaaacac caatacacaa gtatacagaa catacaattg ataattggga tgatatgatg
    40261 ctttttttag caaaaataaa agaacaagga tatgctatag atgatgaaga actagaacat
    40321 ggactaactt gtattggagc acctatctta gataaaaata ataaagctat tgcagccata
    40381 agtttgtcag gtccaactat aagaatgaga gaaggggact ttgagtataa gattaaaaga
    40441 gttatagaaa ctgcaaaaag tatatctgaa ctttttagat gatttttaat ctcgataata
    40501 aaaataggct atatttaaat tgttatttgt aaatatcagt ttaaatgtag cctatttttc
    40561 tttaatctaa aattaaatat tcttaaaatt taaaatgtta ttgaaatata taagcgaatg
    40621 tgttatcttt taattaagca ataaaaatag aacaaggttc tatactatag aacaaaattg
    40681 atgcatatat agtatttata caaaatataa atattatata tttaaaatta agtaaaaata
    40741 ttacataaag gggagatttg agaaatggaa aaagtcaagt tgagtgttga aagatgtaag
    40801 ggatgctatc tttgtattga agcttgtaaa aaagatgcaa taagtctttc tgactacaca
    40861 aataaaaaag gctttgtaac tattaaagtc gatgaagaaa aatgtattca atgtggcgct
    40921 tgttatacaa tgtgccctga tttagtattt gagatattat aaattatttt tattagaggg
    40981 gagaaaaggt atgcaaagtt tattaattaa aggaaatgaa gctttagcag aaggtgctat
    41041 acgtggtggc tgcagatttt atgcaggtta tccaataacg ccacaatcag aaatattaga
    41101 aatgatggca tggagacaaa aagaagttgg aggagtattt atccaaggtg aatcagaaat
    41161 agccagtgta aatatggtta tgggagcaag tgcacttgga gcaagagcta tgacttcttc
    41221 atctggtcca ggattttcat taaaacaaga aggtattgca tattgggtat cagctggttt
    41281 gccagcagtt gtagcatgtg tgcaaagata tggtataggt gatggattta taggagctgg
    41341 tcaagataat tattggcaag cagtaaaagg cggtggaaat ggagattatc atttaatcgt
    41401 gttagcacct aatagtgttc aagaaaatat ggatatggca tatgaatctt ttgaattggc
    41461 tgaaaaatat atgcatccag tacttatact ttctgatgct acaataggtc aaatggtaga
    41521 accttgtata gcaccaccaa tgaaagaata cgatatggat aaagctccat gggctgcaaa
    41581 aggaacacct gtaggagaaa aaaataaagt aataactgat ataacatatt ttgaagacca
    41641 agatacatgg caaaaagggt atatagaaaa aatgagaaaa gttcaagaag atgaacaaag
    41701 atgggaagag atagaaatag aagatgctga agtggtattt gtggcatacg gtatttcatc
    41761 aagaatagca gaaggtgcag ttagagatgc tagagcaaaa ggaatgaaat taggtctaat
    41821 aagaccaaaa acattgtggc cttacccaag aaaagcattt aagaaattaa gtgattcatt
    41881 gaagggactt gtaacagttg aaatgaacat gatggcacag atgaaggaag atgtaattat
    41941 agcatgtgat aatagatttc cagtatattc agtagcaaca gctcaatata tggcagaagt
    42001 aaatcaatta atagattttg ctcaacagat tatagatgga aaagctaagc aagaggaggt
    42061 attttaatta tggctaaaaa agcaccagca ataatgatgt gtgaaaatat gttctgtgga
    42121 ggctgtggac actctatagt cgatagaatt attggagaag tattaacaga aatggatata
    42181 tgtcaagata caattatatg ttcagatata ggttgtaatc atatgtttca atttagtatg
    42241 aatgtagatg ttgtcgttcc acctcatggt aaaatgggag cagcaatggc agctatgaaa
    42301 agagttagac cagataaata tatatttaca atagctggag atggtggagc ttatgcaata
    42361 gggcttggag aaactatgtc agctgctact agaaatgata atgtaatatt tcttgttatg
    42421 aacaataata tgtttgcaat gacaggaggg caaatggctc caactactat agaaggtcaa
    42481 tatacagctt ctactccagc gggtagaaat tacgcagatc atggttcaac atttgatgtt
    42541 gtaaaagtta tgggaaattt agatatagca tatctagcaa gaggaacaat aactaaccct
    42601 aaagaaataa atacaacaaa aagatacatc aaaaaagctc tagaaaaaca aaaagaaaag
    42661 aaaggttttt gcttaataga agtgcttgta ccatgtccaa caaactgggg attaaagcca
    42721 gtagatgcta tgaagagaat tgaaaatgaa cttgttccaa agtatccact tggagaattt
    42781 attgattagg gaggaaataa tatgttgaat gaaattatat gtgctggttt tggaggacag
    42841 ggagttctta cagcaggaaa aattataatg tatgttgcat ataaggcaga aaagaaaatt
    42901 acatggttcc catcttatgg aaatgagatg aggggaggaa gtgcaaattg caatgttgtt
    42961 gtaagtgatg aaaaaattgc aaatccttgt gtaaaaaatc cagatatact attgggaatg
    43021 aacagcgtat cagtagataa gtatgaaagt ttaatgaaaa aaaatggtta tatgtttgtc
    43081 aatgaatcac tagtaagtaa ggatagaaag tacagagaag acattaatgt tataaaagta
    43141 cctgttactg aaatagcaca aaatttaggc aatgaaaaag ctgctaacat ttgcatgtta
    43201 ggtgctatgg taaagagttt ggatttattt acaaaagaag aatttgaaag tggtatgtgt
    43261 gaatactttg aaaatcaagg caaaggtaaa ttcaattcta aaaatgtaga agcatttgaa
    43321 gctggatacg actacgaatg aatattttaa atatttaaaa taattattga aatacttatt
    43381 tttatatgtt agtatttaat taataaaata tagaacgtcg ttctggacaa tgggacgaag
    43441 tctttaggtc ggaatacaag aacggcgttc tgtataatag aactaggggg tgttaaattg
    43501 aaagttttag taataaatcc tggtggtaca tccacaaaaa tttctgtttt ccaagatgaa
    43561 aatgaaattt tgaaaaaaaa tataacccat acaagagaag atttaaaaaa tttttctaaa
    43621 gtatttgatg aatatgatta tagaaaacaa ctgatagtag atattttaag tagtgagaat
    43681 catagtataa attcttttga cgcagtagtt ggtcgtggtg gacttatgaa ggctataaaa
    43741 ggtggtacct atactgtgag tgaagaaatg attgaggata tgagaaatga aataaatggg
    43801 gaacatgcat caaatttagg tgcattatta gctaaaacta tagctgatga aataggagta
    43861 caatcatttg tagtagaccc agtttctgta gatgaatttg atgatgtatc gaggattaca
    43921 ggaatatcag acatagaaaa agcaagttgg ttacatgcat taaaccataa agctgtttca
    43981 agaaaaattg cacaaaaact agggggaaaa tacgaggatt ataactttat tgtagctcat
    44041 ttaggttcag gaatatcgat tgtagcacat aaacaaggca aaatgattga tggaagtggt
    44101 ggtaggtctg atggtccatt ttctccagaa aggagtggtg gattacttgc atatcctctt
    44161 atagaattgt gttactccgg aaaatatact catgatgaaa tggtcgcaaa agtaagttcg
    44221 attgggggaa tgtacgatta tttaggaaca aaggacatga tagaaattga aaacagaatc
    44281 aaggagggtg atgaaaaggc aaaacttata atggatgcat ttatttatca agtagcaaaa
    44341 gaaatttcta tgtatggagc ttcactttca gggaatgtag atagagttat cttgacagga
    44401 gggatatctc actcagaaat ggtagtcaaa ggagtagcag aaaaagtttc atatctagct
    44461 ccaatagaga tagtaccagg agagatggaa atggaggcac ttgctctagg ggcacttaga
    44521 gtacttaatg gagaagaaat agcaaaaact tattaatatt taggagaaag agtatgttaa
    44581 aagattttaa taatttatta gaagaggcta aaaagcttga acctgttatt gtaagtgttg
    44641 ctgttgcaga agacaaagaa gtattaaagg ctgtgaaatc agcaacagat ttaaacatta
    44701 taaagccaat tcttgtagga gacaaggata aaatagaaag tatatcaaga gaaattggat
    44761 taaaaggtca tgaaataata ggttgtcaaa atcccgatga atcagttaaa aaagctgtag
    44821 agatagttaa aaataaggaa gctaaagttc ttatgaaggg attagtaaat acaagtgtct
    44881 atatgagagc tatattaaat aaagaaacgg gtcttagaac aggaagatta ttaagtttgc
    44941 ttgcagtata tgaattacct caatatcata aacttcttta ttgtactgat agtggaataa
    45001 atgtatcgcc aaacttaaat caaaaaaaag atataatgac aaatgcactt cttactatga
    45061 aaagtatagg catggaaaat cctaaaactg cgatacttac tgcaaatgaa atggtagacc
    45121 ctaagataca atctacagtg gatgccaacc tacttgtaga gatggaaaaa tcgggagaaa
    45181 taccaaagtg tatagtggaa ggtccaatag catttgatgt agcatttgat gcacatgcag
    45241 cagaacataa aggtattaaa agtaatattg ctggtgatgt tgatttatta gtatttccaa
    45301 atatagaagc agggaatata ttaggaaagt catggcttag atttaataaa gctaagtggg
    45361 caggtatagt tttaggagca acaaatccga taattttagg ttcacgttct gacacagctg
    45421 aaataaaaat aaattcaata gcattagcgt gcctagcatc acaataagaa attattatta
    45481 aggaggaaaa agtattatga aatatgcaaa aagaatggat atggtaaaag cttcagctat
    45541 aagagattct caaaagaaaa tagctcagaa ggtagcaagt ggtggaactg ttatatcttt
    45601 tgctgcaggg cttcctgacc caaatttatt tccaatagaa gagataagtg aaataactca
    45661 aaaagtactt aatgaaaggg gaaaatttgc cctagcatat ggacctacta aaggtgaaga
    45721 tgagttgtta gatctattag taaaaagaat gaaagaagaa gaaaatattg atacaaaagc
    45781 agaaaatatt ataataacta ctgggtcaca acaagggata gcattaagtg ctatgatttt
    45841 attagaaaag ggagatgtag tagttacaga aaatccaagt tacttaggtg caataaatgc
    45901 atttagacct tatgaatgtg attttttagg agtagataca gatgaagaag gaatggtaac
    45961 agatgattta gacaaattat tatctgaaaa ttctaacata aaggtgatat atgtaatacc
    46021 aacatttcaa aaccctacag gaaaaacatg gtctttacaa agaagaaaag attttatgga
    46081 agtcgtgaat aaatatgatg tcatagtaat agaggataat ccttatggag aaataagatt
    46141 tacaccagaa cctctaccta cacttaaatc tcttgatact aaagataaag tattgtacct
    46201 tgggtcattt tcaaaggtat tatgtccagg atttagagtt gcttggatgt gtggtaatgt
    46261 agacatcata gataaggcag aacttttaaa acaaggtata gatttacagt caaatcaatt
    46321 ttcgcaagta caagtaatag aatttttaaa gaaatacaac ttaaatgaac atatagaaaa
    46381 aataagagca gagtataaga aaagatgtct gctaatgtta gatgctatga agaaacattt
    46441 cccagaggaa gctaaatata cagaaccaga tggaggaatg tttatatggg ttgaacttcc
    46501 tgaaagtata aatgtagatg atttattaga taaagccatt gatgctggag ttgcttatgt
    46561 ttctggagag tccttctttg caaataatgg tccaaaaaat actatgagac ttaactatac
    46621 aacaatgtca gaagaacaaa tcgttaaagg tgttgagata cttgcagaag ttataaaaaa
    46681 agaactcaat tatgcttgtg aataaatata aaaagtgagg gtttaaatat gggagaaaaa
    46741 gaaaacggta taaagtttgg aaagttaatg tttcaacttg ttattatagc aatatcttgg
    46801 gaattagttt atattatacc atttatccaa tatacattat atgatccaat cctaaaagca
    46861 ttagaatgta caaacactca actaggtttt ctacttacaa tttatgggtt aggtaatata
    46921 tttggagctc ctataggagg ctggttagca gatagatttg attatagaaa gataatttta
    46981 ggttctgtat ttcttaatgg agtagtatca tttttatttg catttaatat gaactatcca
    47041 tttgcagttg ctacttgggt aggttgtgca atcacatcat tagttatgaa ctatccttct
    47101 atggtaaaaa tacttagagt tataggaaaa gataatcaag gtaaggttta tggatttaat
    47161 gaagctatgg ttggagtttc tggagtagta atgggagcaa tattcttata tatttataca
    47221 tgctttgcaa ctcctacatt gggtatgaga tgggtaatga tatctttagg aatattatca
    47281 atagcaatgt gtccagtatt atggtttgta attaaagatg tagatattaa agaagaaaga
    47341 gaagaagcag gtgaaaaaat gtctgctggt gattttataa cagtactaaa atcaccaaat
    47401 acttggctag taggaataag tatattctgt gtatattcat tcacagttac aatgtcttat
    47461 tttacaccat atataacatc tgtacttgga ggttctgtag cattatctgg tgcattagca
    47521 ataataagac agcatggtct aaaattattt ggagcaccat ttggaggata ttgtgcagac
    47581 aaattaaaat ctcctacaaa agtactttta ccaatatatg tatttggtat agcagtaata
    47641 gttttattcc tagtattacc tgcatcaact ccaatgacta tattcatagc tttaacattt
    47701 gtagtaggta tattaggtta catgggaaaa ggtatatatt atgcagtaca agatgaagta
    47761 aaagttccag ttaaatattc agcaactact ataggtatag ctgcagcttt aggattttca
    47821 ccagatgtat tccaatttgc attaatagga cactggatag atacttatgg caataaaggt
    47881 tacacatata catttatatt ccaaatagca attctagtta ttggaatact atcttgttta
    47941 tatatattaa aggttaaaaa gagaagagca agtaaattag aagcttaaat attcaatttg
    48001 gtaattaagt atatataaat acaatactta ttttgatgaa tgtgattgta taaataacat
    48061 aaaaaatatc aatagattaa gttttataat aatgtttaga gaataaagta taaaaatcaa
    48121 aatgttatca agttttctag gttttaacat aaagttaccc ctgaaggtgt aaaattgagt
    48181 ggcaattatt atctattttc ttacctacat aaacctattt aatttaaatt ctaagagatt
    48241 ttcaaaatag atggtagttg tactcaattt taaacctcat aataaatacg aaaatttaaa
    48301 atctaactag atactattta attatatatt aataaatata tattgtacta tttatataat
    48361 aataatttca ggcttttcat tatatgtatt ccaatttata ttgtttgatt attattcaaa
    48421 cacacgtcgt taaaaatttt ataaatttat gtttagatat gattaatact cttgatttta
//
LOCUS       DAMJZE010000012        46054 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJZE010000012 DAMJZE010000000
VERSION     DAMJZE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.012"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            138..521
                     /locus_tag="PC541_000581"
     CDS             138..521
                     /locus_tag="PC541_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2838061.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            523..873
                     /locus_tag="PC541_000582"
     CDS             523..873
                     /locus_tag="PC541_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838062.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(974..1432)
                     /locus_tag="PC541_000583"
     CDS             complement(974..1432)
                     /locus_tag="PC541_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838063.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1477..2265)
                     /locus_tag="PC541_000584"
     CDS             complement(1477..2265)
                     /locus_tag="PC541_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2838064.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2404..2841)
                     /locus_tag="PC541_000585"
     CDS             complement(2404..2841)
                     /locus_tag="PC541_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2838065.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3663..4172
                     /locus_tag="PC541_000586"
     CDS             3663..4172
                     /locus_tag="PC541_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838066.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4578..5114
                     /locus_tag="PC541_000587"
     CDS             4578..5114
                     /locus_tag="PC541_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2838067.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(5541..6341)
                     /locus_tag="PC541_000588"
     CDS             complement(5541..6341)
                     /locus_tag="PC541_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838068.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6536..8134)
                     /locus_tag="PC541_000589"
     CDS             complement(6536..8134)
                     /locus_tag="PC541_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2838069.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8462..8638
                     /locus_tag="PC541_000590"
     CDS             8462..8638
                     /locus_tag="PC541_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838070.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            8812..9942
                     /locus_tag="PC541_000591"
     CDS             8812..9942
                     /locus_tag="PC541_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2838071.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            10183..10533
                     /locus_tag="PC541_000592"
     CDS             10183..10533
                     /locus_tag="PC541_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2838072.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10541..10990
                     /locus_tag="PC541_000593"
     CDS             10541..10990
                     /locus_tag="PC541_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2838073.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11092..11667
                     /locus_tag="PC541_000594"
     CDS             11092..11667
                     /locus_tag="PC541_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2838074.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            11660..13069
                     /locus_tag="PC541_000595"
     CDS             11660..13069
                     /locus_tag="PC541_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2838075.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            13253..14686
                     /gene="eutA"
                     /locus_tag="PC541_000596"
     CDS             13253..14686
                     /gene="eutA"
                     /locus_tag="PC541_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2838076.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            14704..16068
                     /locus_tag="PC541_000597"
     CDS             14704..16068
                     /locus_tag="PC541_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2838077.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16081..16962
                     /gene="eutC"
                     /locus_tag="PC541_000598"
     CDS             16081..16962
                     /gene="eutC"
                     /locus_tag="PC541_000598"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2838078.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            16982..17635
                     /gene="eutL"
                     /locus_tag="PC541_000599"
     CDS             16982..17635
                     /gene="eutL"
                     /locus_tag="PC541_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2838079.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            17646..18347
                     /locus_tag="PC541_000600"
     CDS             17646..18347
                     /locus_tag="PC541_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2838080.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            18350..19819
                     /locus_tag="PC541_000601"
     CDS             18350..19819
                     /locus_tag="PC541_000601"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2838081.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            19915..20202
                     /gene="eutM"
                     /locus_tag="PC541_000602"
     CDS             19915..20202
                     /gene="eutM"
                     /locus_tag="PC541_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2838082.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20329..21090
                     /locus_tag="PC541_000603"
     CDS             20329..21090
                     /locus_tag="PC541_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2838083.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21103..21732
                     /locus_tag="PC541_000604"
     CDS             21103..21732
                     /locus_tag="PC541_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2838084.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            21774..22499
                     /locus_tag="PC541_000605"
     CDS             21774..22499
                     /locus_tag="PC541_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2838085.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            22512..22784
                     /locus_tag="PC541_000606"
     CDS             22512..22784
                     /locus_tag="PC541_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2838086.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            22777..23328
                     /locus_tag="PC541_000607"
     CDS             22777..23328
                     /locus_tag="PC541_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2838087.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23340..24431
                     /gene="eutH"
                     /locus_tag="PC541_000608"
     CDS             23340..24431
                     /gene="eutH"
                     /locus_tag="PC541_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2838088.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            24433..24906
                     /locus_tag="PC541_000609"
     CDS             24433..24906
                     /locus_tag="PC541_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2838089.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25026..25127)
                     /locus_tag="PC541_000610"
                     /pseudo
     CDS             complement(25026..25127)
                     /locus_tag="PC541_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            25422..25943
                     /locus_tag="PC541_000611"
     CDS             25422..25943
                     /locus_tag="PC541_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2838090.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            26322..27497
                     /locus_tag="PC541_000612"
     CDS             26322..27497
                     /locus_tag="PC541_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2838091.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            27532..29070
                     /locus_tag="PC541_000613"
     CDS             27532..29070
                     /locus_tag="PC541_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2838092.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            29102..30304
                     /locus_tag="PC541_000614"
     CDS             29102..30304
                     /locus_tag="PC541_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2838093.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(30471..31070)
                     /locus_tag="PC541_000615"
     CDS             complement(30471..31070)
                     /locus_tag="PC541_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2838094.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            31240..32598
                     /locus_tag="PC541_000616"
     CDS             31240..32598
                     /locus_tag="PC541_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838095.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            32591..33223
                     /locus_tag="PC541_000617"
     CDS             32591..33223
                     /locus_tag="PC541_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2838096.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            33552..34709
                     /locus_tag="PC541_000618"
     CDS             33552..34709
                     /locus_tag="PC541_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2838097.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(34856..35491)
                     /gene="lexA"
                     /locus_tag="PC541_000619"
     CDS             complement(34856..35491)
                     /gene="lexA"
                     /locus_tag="PC541_000619"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2838098.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(35595..36722)
                     /locus_tag="PC541_000620"
     CDS             complement(35595..36722)
                     /locus_tag="PC541_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2838099.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(37033..37878)
                     /locus_tag="PC541_000621"
     CDS             complement(37033..37878)
                     /locus_tag="PC541_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838100.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(37908..38279)
                     /locus_tag="PC541_000622"
     CDS             complement(37908..38279)
                     /locus_tag="PC541_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2838101.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(38432..38692)
                     /locus_tag="PC541_000623"
     CDS             complement(38432..38692)
                     /locus_tag="PC541_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2838102.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(38881..39825)
                     /locus_tag="PC541_000624"
     CDS             complement(38881..39825)
                     /locus_tag="PC541_000624"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2838103.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(39846..40700)
                     /locus_tag="PC541_000625"
     CDS             complement(39846..40700)
                     /locus_tag="PC541_000625"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2838104.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(40715..42076)
                     /gene="accC"
                     /locus_tag="PC541_000626"
     CDS             complement(40715..42076)
                     /gene="accC"
                     /locus_tag="PC541_000626"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2838105.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(42092..42544)
                     /gene="accB"
                     /locus_tag="PC541_000627"
     CDS             complement(42092..42544)
                     /gene="accB"
                     /locus_tag="PC541_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2838106.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(42814..43260)
                     /locus_tag="PC541_000628"
     CDS             complement(42814..43260)
                     /locus_tag="PC541_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838107.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            43535..43813
                     /locus_tag="PC541_000629"
     CDS             43535..43813
                     /locus_tag="PC541_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838108.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(43942..44688)
                     /locus_tag="PC541_000630"
     CDS             complement(43942..44688)
                     /locus_tag="PC541_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838109.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGKAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(44817..45461)
                     /gene="trmB"
                     /locus_tag="PC541_000631"
     CDS             complement(44817..45461)
                     /gene="trmB"
                     /locus_tag="PC541_000631"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2838110.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(45599..45919)
                     /locus_tag="PC541_000632"
     CDS             complement(45599..45919)
                     /locus_tag="PC541_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2838111.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        17382 a         6134 c         7046 g        15492 t
ORIGIN      
        1 aaaatttaaa ttgtaatgaa taggaagtaa ttaaaaagaa tttttattga tttttaaaaa
       61 tttagttaga aactatatca ttatggtaaa aaatgtatta taatgtttag aaatattata
      121 ataaggagcg attaaacatg aatctatcga agaaaataga agataaaaga aaagctaaac
      181 agagagctga aaaagttaag aaagctaaaa ttgctacagc tggtgtggta ttaggagctg
      241 ttactggtgc agtgagtgga gtacttttgg ctcctaaatc tggaaaagaa acaagagagg
      301 atataaaaga tgcttcacaa caaattgctg aaaagataaa tatgaaaact gtagatgtaa
      361 aaggcaaagt atctgagaag ctagaggata aaaaaggaaa ctttatagaa tcaaagaaaa
      421 aaataaaaaa atatatagat aacaaaaaga gtgttgaaaa ttctagtgag gaagataaaa
      481 taatagatat agttgaacca gttagtgtta ctgaggaata atatggacaa tacaataacg
      541 attaacataa gtataatata tgtattatta gctatacttg cttgtattgc tttagtttat
      601 ttaattattg tacttgttaa actacataag attttaaata atgttggaac tatgttaaat
      661 gaaaatactc aaaatataaa tacaacttta aataatcttc ctaatgttgt agaaactttt
      721 ggagaagtag gagaaaatat gaaagatgta acagaagtag ttactgaagt tacagcagat
      781 tttcttgtta ctaaagacag tgtcaaatca aatatggatg ttatagttga tatattgact
      841 ataattaaaa acatttttct agataaaaaa taataagttt aattaaaata agcctataag
      901 atataattta aacgacctta aaatagcatt tataaggtcg ttttttatta gcttgatttg
      961 acactatgtg tttttaataa ttggtaatat tcctctttat catttgaaca tatatatcta
     1021 tgcttgttgt ttcaattaca tccataagaa catctttaca ttcaagctca aaatcttcca
     1081 ttccttctaa atattcatca tcttcatcaa aaaagatata agaattatat agccattctt
     1141 caaatttttc ttcaagtata tcccaatacg aactccaaaa taatccacaa cctgatatat
     1201 aagatgctct actttgattt atataaacct cctctagaag cttttgtaat ctactgctat
     1261 aaagaaaatc tgctcttcca ctgcttgata ttaattcatt taaatcttct attgacatta
     1321 attcaacttt accactttta tcataaggag tataattttc atcaacccat ttatgtggtt
     1381 tgttttctct gcatactgtt gctatcaact ctacaacttt tgttcttatc atttttttat
     1441 cctaacttat ttatttttta taaaataatc atgattttat aatttatcta tttcttgtaa
     1501 tgctaattta acagtttcat atctatcagc accatttatt actacatcac cttttacaag
     1561 tttattagct tctccaccta caacataaac ctttttacct aaaccttgct tataactaga
     1621 cgcttcaatt attaaagaat ctttcaattg ccaatgtaaa atctgagcag ccactttatc
     1681 agcatcacct gtataaacga ttacattttc atacacttta ttttcccctc cctcttgaga
     1741 atttgatata gatttgttta gtataccttc tgctatcaat ttagcaactt tatctttatt
     1801 attaacataa taatctgtat ctgctttact atctacaaaa catacttcta ttaatattgc
     1861 tggtgcaata gtatgattaa gccaataaag acctctaaca tcagactttg cgcccctatc
     1921 tttgaatact gtagctaacc tagtattcac tctttctgca taggttttgc cattattagt
     1981 cttatatatt gtttctgtcc ctactggagt tgaagttgtg gcatttgaat taaaatgaat
     2041 ctgaacagcc aaatcagttt cctgtttatt tgctatagca cattggtctg ctaaatgatt
     2101 acttgattca tcaactcttc ctgtataaac agtagctcca cctattttaa gccatttaac
     2161 aattaagtca ttcagtattc ttgtttcttt gctttcgttt atatacccaa cagctcctgt
     2221 accttttcca attaaagtgt gccctggtat tattactact ttcaaactaa catctccttt
     2281 acaattaaat ttaacttcta tttaatatat gcataaccta gaattttgtt tctttttata
     2341 tcttatactt ttattataac ccattttgta aaaatataga atattaagta aataatgtta
     2401 aaattataat tttgtatata agacattact tgattgcaat aattttatag catattcatc
     2461 tattctatat ccagttctat aatatatatg tttaattcca gcttgaatta tagccttagt
     2521 acaatttaag caaggaaagt gagttacata aatagagcaa tttttaactg aaataccttc
     2581 ttttgcacaa taataaaggg cattttgttc agcgtgaatg gttctaatac aatgtccatc
     2641 tctcatttca tgaccaactt cactacaatg cttatctcca gaaatactac cattatatcc
     2701 agtagacact attctgtttt ctgaattgac aattatagct cctacataag ctctatcaca
     2761 agttcctctt tcagctactg tttcgcacag tctcataaaa tattcttgcc atgagcatct
     2821 attttcaagt tttttattca aattatatac ctccaaaact tatatctaat atataagatt
     2881 tataccttaa aaataatatt aaaaacaaat ttatacgata aataaaattt attaatactg
     2941 tttttagtct aaattatttc attttaaatt aaagttacaa ggaatctaaa attatatata
     3001 tctttcttcg taatattaat gttacaatat aaaaaactgt ctcaataaaa tagagacagt
     3061 tttacagtca aagtttataa tacaagttca tatttttctt taaattcttt tacagcttcg
     3121 ggccatgcct tttgttctgt aatacctttt tctttagcta ttttaattgc aatctctctt
     3181 atcattgcag cgccttctaa tattcccatt tttaatctcc cttcaaatat ctttttgtat
     3241 agaatataaa tagtgctaag atttattaca tgctgcatat acaatatcta tacaacacat
     3301 aacttgtaaa ttccttttat tttcatacgt ctttaaccga catataatta tattcaataa
     3361 gttttaaaat tatgaactca attataatta tttttatttt tttagaatct aaacactaaa
     3421 aattgtaggt aaatttgtct tatgccttta taaatatttc tacagtttta attactggac
     3481 atatctattt ttattataaa aagcgattaa aaatcgagaa tatccgcata aaaactgtat
     3541 ataagcaaaa cggttaaagg aaaaagaaga aatttacttg aatgttaaaa tgcgttgctt
     3601 gaagcaatgg gcagttttgc ctataatatc ggtaacaact gaaagaaagg tggttagccc
     3661 taatgttaag cgaaaacatt aaaacaatca ggaagtcaaa aggactttca caacaagaac
     3721 ttgctataaa actgaatgta gtgcgacaaa caatatctaa atgggaacaa ggactgtcag
     3781 ttcccgattc tgatatgttg atttccatat cagaggtgct tgaaacgccc gtaagtactt
     3841 tacttggaga aactattgtt gagtcggaag ttgataactt aaaagtgatt tctgaaaaat
     3901 tagagattat aaacttgcaa cttgcacaaa gaaaaaacat gagaagaaaa atgtttcatt
     3961 ggttatttat tttattatgt gcagtcatat taatagtttt tgtggtctta gggggactaa
     4021 atagtcctta cttggattgg aattataccg accctgaaac cgctgttttt ggagtagctt
     4081 ttcatgcttt tgagtggcta tttgtcagat tagtaccgat tgtccttata ggagcaatta
     4141 ttggaatctt tttgacacgt aagaaaatat gaagtaactc tgctttttat aatacagcaa
     4201 gcgttcctta aaagcgtcat tctacatggc aatcgaggac gactatgtaa ggaagaacct
     4261 ttttgatttc caactaagcg aagttattga ggacgacagt aagaccaaaa ccgcattaag
     4321 taaagtacag gaagcaagac tgctggactt atactctcat gcgtcagatg caggaaatgc
     4381 gaggtctggt agcatgattt acaacgattt gtacaacgac tgctagtaga aataatatgg
     4441 agataagtat tatctacaaa gtcagaaaag aaatgacttg ataatttact catgttataa
     4501 ttattgatgt gaaattaata aatcgaaatt ccttttttgg atttaataat actgagtaag
     4561 gaaaaagtga attacttttg caaggaggtg ttaatatcag gcaagtaaag tcttttgttt
     4621 ttccagttat atccttaata ttcttagacc aaattagcaa agttcttata ggattattct
     4681 taatggactt tgaaattgat ataattggga aatttttaag attcaatcct gttcaaaata
     4741 caaatctctc ttatggggga aactttattg gtattctatc taatttatgg gtattggtat
     4801 tgtttaacat tttagttata ttagttatta tatctggata tgctttttat aaatcaaaaa
     4861 atgaacaaac aagctattca gtaaaagtaa ttatgtcttg tggacttgct ggtacaatat
     4921 gtagcttgat agataaatta ttttggggag gaagtttaga ttttttgcag ataccaagct
     4981 tttttatttt cgacttaaaa gactgctacc ttactgttgc agaaataata tttgttgtca
     5041 taggaatttt gcataataga gaaatatcaa tgaaagaata catatatttt tgctatcgtc
     5101 agtttaaaaa ataaatttag aagataaatt taagtttcaa ggtaaactta tttaatcaca
     5161 gcaaatagtt ggcttactta agcttttggg ctaaactgtg agcactaatt aagtctctga
     5221 tatctaacaa acggagcaca aaaataaaaa ttaaatataa agcacagtta attttgatta
     5281 aaaagttgac tgtcttttct tttgtagaaa tttaagcata ggtaataaag aaatgactgc
     5341 atttaagcat tatgtatata attggaggca ataaaaagcc agcaaagccc ataaaataga
     5401 gctgtgctag caacatgatg aattataaga agataatcaa aaacttacgc tttattttaa
     5461 tttaaaaaaa tgattactgt aaagaaatca ttttttatta taatcactga tacatcaaat
     5521 attctaaata aaatttttat ttatctggaa ttgtatattt tttctataat attaactaca
     5581 gcgtacattg cccaagcaca gatagccaat acaaatactc ccatcattac taaatctaat
     5641 ttaaatactt gagaaccata tactatcaag tagcctattc catatcttga aactaaaaat
     5701 tctccaacta ttacacctac ccatgccata ccaatattaa ttttagttaa atttattaaa
     5761 tttcctgtgt ttgcaggtaa tatgagttta aataatattt gagatttagt agctccaaaa
     5821 ctttttaaca tcttaatttt ttcttcatct atattcataa aataattata agctgaaagt
     5881 atagtaacaa caacagaaat cgttacagct gttacgacga ttccttctat tccagcacct
     5941 acccaaacta taattatagg agctaaagca gtcttaggaa gagcatttaa aactaccaaa
     6001 aatgggtcta atatttttga taatttttct gaccaccaaa gagcaattgc taccaaaata
     6061 cctaatacag tacctataac aagaccaaga acagtttcat atacagatat tccaatatgt
     6121 ttaaataatt gaccactaaa tgcatattga ataaataaat tgtaaatatc acttggtttg
     6181 ctaaatagaa atgtatttat aacatttaag tcagctaaaa gttcccagat tacaataaaa
     6241 ccaattaaaa ttaatagctg gtagaataat acatttcttt tttctctttt tacaccttta
     6301 acatagttta tatatccttc agatctttta ttaagcctca tgtgtatcta gctccttcca
     6361 taatatgtcg aaatactttt gaaaatcagg aactttacgt gttttgagag gcgtttttgt
     6421 tccatccatg ttaagttcaa tatcgtaaat attttttatg gtagctggtc ttttagatag
     6481 tattgcaacc ttatctgata tgcttattgc ttctgctata gctaaaaaag tgcaactaat
     6541 tgcttaaaga aacagaatta tcaacttcag cacccatata aacaatatta agatttccta
     6601 aactgctatc ccaaacgata ctattaataa tagtagataa taacaatctc ttatcatcta
     6661 aactggcatt atcaacttca tcattaaatc tcttcaaatt actaacaata atatcaatat
     6721 taatactgtc actactaata ttgatgttac tagattccaa agtattaatc ttatccttta
     6781 aaatactaat ctcattattt aggctctcaa tctgggaaac tatataatca gaagcagtac
     6841 taccagtatt ttttgcaagc tctataacta aatttttaat aagtctttcc ttatctaaaa
     6901 tttgattttt aagtaaatta gtctcagatt taaaaatatt aaaaacatta tcagtcttaa
     6961 tgttattact attgaatgca cttataatag actcttcttt acaattctta attttatcta
     7021 taactaaact ttcaactaat ggaccattaa gatttttaca gttgcaacta tcagacccaa
     7081 gtgttttttt agtaccacaa atataataat actttaaatc cttgtcagtt tttttgcctt
     7141 tataggtaat tctcatatta gaaccacatt taccacattt tataagacca gataaaagag
     7201 cagtttttcc agttgatatc cttggagttt tatcacgatt tttatctaat tgattttgta
     7261 cttcaagcca tatttcaggt tctatgactc catggtgttt tgcaattgct gctattgagt
     7321 tagatgtatt tttagcatag gtaaggattc cacattttct atctggagta cctacaacat
     7381 ccataccact atttttaagg taatctaaaa tttcatcatt agcttttaca tatgctggat
     7441 ttctaagtat tctagataaa gaactagggt caaatttgcc accacgagga ccccttattc
     7501 cattattata gacatattta tataacttag ataaggacct aagttctaaa tatttattgt
     7561 atattagctt tacaatctcc atttgttcat catttacttg aagcttatac atacttcttt
     7621 cttttagatt ttcatctaag aatgtgattt tttcactgtt gaatccatga ggtgtattgc
     7681 ctccaagcca tctaccacta cgggctaatt catacatatt gtcttttata cgttctgcta
     7741 ttgtttctct ttctaattga gcaaatacac ttgatataaa catcatagct cttcccattg
     7801 gagttgaagt atcaaattgt tctttgatag atataaatga tatgcttagt tcatttaatt
     7861 tttctattaa agtagagaag tcagatacat ttctcgatat cctatctaat ctataacaaa
     7921 ctaggtaact aaatttctta tctttggcat cctgcatcat tttcttaaat tgtggtctat
     7981 ctatattgct accactaaat ccctcatctt catatatcaa aaaatctttt acaccaatag
     8041 actttgcata atcaatacat aattgtattt gattttctat agaatcacct tttccagtaa
     8101 atcttgattt tcttgaataa attgcagctt tcaaaattaa aaccccctat aagttaggat
     8161 atatatttga aactatttat ctacaacctt ttaagtaaac ttattagaat gaatttatta
     8221 attaggtaaa tttatataat tctacttttt ataatatatg actatttatt ttatttcttt
     8281 actattcaag ttctaaatca ataataaatt ttgatattta attgtatcag gacaaatctc
     8341 taatttcata gattattaat ataaaatcat aggttagtgt gaaaaagttg aaatttaaat
     8401 attttaaaag ataatttttt tgaactaata ggtataacaa aaatttatag ggggattgca
     8461 tatggaaatc aatgttatag aaattttccc taaagataaa gctaaactta ataaaataga
     8521 aatggataaa gctagttggt ttgtaaatat aataagtaaa aaatatccta aagaagcttt
     8581 aaatgaagca tttagtactt tagaaaaaga attaaatata agtaaagcta atacataagc
     8641 tttgattatt gattttatat actattggtt ttatacgcta tgacttgaca ttatattaat
     8701 caaatagtat atttttaaat atagcacaat gaagtgttaa aagagcaatg gagctccata
     8761 gataaagtct atggagcttt tttgcgtatt taacttaagg aggataggac tttgaataac
     8821 tttgagatta aaactagaat aaaatttgga gaaggctcac taaaagcttt gcaagaatta
     8881 aaaaacaaaa atgtactaat aataacagac ccttttatgg tgaaatcaaa aacaatagat
     8941 aaaataacat ctaatttaat aaactcaaca tatcaagttt ttgaagaaat agtaccagac
     9001 ccaccaatag aagtagtagt agcaggaata gaagcattta aatctataaa tccagacgta
     9061 ataatagcat taggtggtgg ttctgcaata gatgcagcaa aatctataat ggacttttct
     9121 aaaaaaatat taaaaattaa cgaagtagag tttatagcaa taccaacaac aagtggaaca
     9181 ggttcagaag taacatcatt tgctgtaata acagatagtc aaaaaaaatg taagtatcca
     9241 ttagtgagtg atgatttact tccagatatg gcaatactag acccagaact tgtaaaaact
     9301 gtaccaaatt ttataacagc agatacaggg atggatgtat tgacacatgc tatagaagct
     9361 tacgtatcta caaattctaa tgacttttca gatgcactag ctgaaaaagc tataaagtta
     9421 gtatttaaat acttaataaa agcatataaa gatggaaatg acatagaagc tagagaaaaa
     9481 atgcacaatg catcttgttt agcaggtatg gcatttaacc aagcatcact tggcataaat
     9541 cattctattg cacatgtatt aggtggaaaa ttccatatac cacatggaag aacaaactct
     9601 atattacttc ctcatgtaat agaatataat tcatcaatag taggatatac taacacaact
     9661 tactctgcta cagctaaaaa atatgcacaa atagcagaat tagtaggact tgggagtgct
     9721 aatataagaa tatctgttag aaatttagta aatgaaataa agaaactaca aaaagaaatg
     9781 aatatgccaa ctaaattgac acaatgcaaa ttaagtttag aagatataac taatgaagaa
     9841 agagaaatag caaaacttgc tataaaagat acttgtacat taactaatcc taaggtacca
     9901 actgaagatg atataataaa catattaaag aacattaaat aactacattt tattacatgt
     9961 aaatataata ttatacaaat agggaaaatg tttttaaaaa tataaattat atattgacaa
    10021 atataaaaca taaaactata atgaaagtat cacaaaggcg tgatacttta gtagaaagca
    10081 atggagcttc ttaggaatat taatattatt cttatggagc tccttttatt ttaatcaaat
    10141 agctttttaa taaactatat tcaaagaaag cagggaataa atttgactga agaatctaag
    10201 caaagagtta ttcaagaata tgttcctgga aaacaggtta cattagcaca tatcatagct
    10261 aaccctaatg aagatatata taagaagtta ggattagtac ttgataaaaa agatgcaata
    10321 ggaatactta caataacacc aagtgaagcg tcaataatag cagcagatgt tgctacaaag
    10381 gcttcaaatg tatctttagg ttttatagat agatttagtg gttcagtagt aatatcaggt
    10441 gatgtaagtt ctgtagaatc agcgttaaat gatgttttag aagtcttagg aaatatgtta
    10501 aacttctcat ctacaaaaat cacaaggaca taataataca atgaagaata taatatttat
    10561 gggaaagaca ggtagtggta aaactacctt atgtcaaaaa ctagaccagt tagaattaaa
    10621 gtataaaaaa actcaatcag tggaacttta taataatgca atagacactc ctggtgagta
    10681 tatggaaaat agaaacctat ataatgcctt aataacaaca tcagcagatg ctaaaattat
    10741 agcaatagtg tatgatgcca cacaagaaga aaattatata gcaccaggtt ttgccagtat
    10801 gttttgcaaa gacgtaatag gaattataac taaaataaat aaagctactg aaaacgaagt
    10861 caatatagct tatgaaagac tatcactagc aggagcaagt caaatcttta aaatagacac
    10921 agttgataat attggacttg aaaaattatt taaatatcta gaaaaatata aaaatataga
    10981 tgaagtttaa ggggaaatat ttttttattc aaaaatatta ttaaagtatg ataaaataaa
    11041 aaaatgtata aactatttta agaagattta atctttaagc gaggtaggaa catggggaaa
    11101 aaaatactat tggttgatga tgaaccactg ttaaggatgg atacaaaaga tatattagaa
    11161 agtcatggct atgaaataat aggcgaagca agtgatggat ttgaagctgt tgaaatgtgt
    11221 aaaaaacaca aacctgattt agttataatg gatatagaga tgccaatact tgatggtata
    11281 aaggcaggca aaataatatg taaagaaaat atagctggtg gagtactttt attaacttca
    11341 tttgaagata aagaatacgt agaaaaggct aaaagtatag gtgcttatgg atatttagta
    11401 aaaacagcaa gtgaaaaatc acttatccca accatagaaa tgtgtttaaa taaggtaaat
    11461 gaatttgaaa aaatgaaaaa agatttagaa aaagtaaatt ctaaattgaa tgaaagaaaa
    11521 ttagtagaaa gagcaaaagg tatacttata agagagttta agatttctga ggatgaagca
    11581 tacactagaa taagaaaatt aagtatggat aaaagaacta caatgatgga aattgccaaa
    11641 atgattataa taggatacga tgactaatac gataaaaaaa atatcaactg cacaaacata
    11701 cttaaataat aacgaaattg aaaaaattat agaagtatca aaatccttag atttaatggc
    11761 ttctttttat gaagctgatg tttttataga tgtactaagt aaatttgatg atgaagcaat
    11821 tgttgtggca catggtagac ctaaagaaaa gtcaatgtac tgtgaaaatg ttataggtaa
    11881 aaaggcttta attgaaaatg aaccaggagt tataaagtgc ttaaaaacag gagaaagagt
    11941 aagagatatt aaagctttaa cacaggaata taaactggtc aaacaaaata ttcagccaat
    12001 aacactagat gacaaagttt tagcagtact aatagttgaa aaagatataa gtagtgaagt
    12061 taagaatgag tttaatgcta gtgaagacga agaaaataaa aatttacatc agtttataaa
    12121 acttattgga aataatgact ttttagttga taatttaaat tcagccatat taatatttga
    12181 taaaaatgga aaattaaaaa ttaaaaatcg tattgcaact gagatataca aaaatcttgg
    12241 gtttggatat gatatacaag atatgcatta caatgattta gatatagaaa ccaacacctt
    12301 tgaaaatata ttaatggatg aaaattatga tgagacaaat gaagtttcaa taggtaatat
    12361 gttttttaaa ataaagacca tatacatcaa agatgatgaa tttaaggttt gcaaaatagt
    12421 acaagatata agtgatttaa agagaaaaga agaagaatta gttttgaaaa cagtggctat
    12481 aagagaagct catcatagag taaaaaataa tcttcataca gtaatatcta taataaaaaa
    12541 acaaagtaga ttatctcaaa atgatgaagt aaagcagtgt ttagataaca taataaatag
    12601 ggtatttgca atactttcga ctcattattt actatctaaa gaagttgata ataatatatc
    12661 tatacgagaa ggtatcaatt tattagtgtc aaatattcaa ggtggctatt tatttagtaa
    12721 ggatatagat atacgtataa ctggtgaaga ttttaaaatt agtggagaaa aagcaacagc
    12781 aatacttttg gttataaatg aaatactaca aaattgttat gaccatgcat tttcggatag
    12841 agaacatggt actatacaaa tattaataaa taaagataac gactatagaa atattgctat
    12901 tatagatgat ggaattggat ttgatagtaa taatataaat aaaaatagct tagggacata
    12961 tataatagat agttatataa cacagatgtt aaaaggaaat atagagagaa aaagtagtga
    13021 gaatggtaca gaagtattaa ttactatacc tacacaaatt aatatttaaa taataactca
    13081 tctacaaagt agtagtttta attgagcaaa gaggcttaag gttattaata gattaagtta
    13141 attgaatgta tttataaaaa ttataagtat atattcaatt taacaggtat ttattataac
    13201 tttaagcctc tttgcttgtt taagacatac ttaaatttta ggaggtgaga aaatgagaga
    13261 agagttatta agtgttggta ttgacattgg aacaagtact actcagttaa ttttttctaa
    13321 attaatagta gaaaatactg aatcaaattt ttcggtacct agaataagta tagtagacaa
    13381 agaaattatt tataaaagca aagtttattt cacccctctt atttcaaata atgaaataaa
    13441 tggtcaacaa ataaaagata tagttgaaaa tgaatataaa aaagcaaata tatctaaaga
    13501 agatatacat acaggagcag taataataac tggtgaaaca gctagaaaag aaaatgcaaa
    13561 tgatgtactt catactttga gtggatttgc tggggacttc gttgttgcaa cagcaggacc
    13621 tgatttagaa agtataatat caggaaaagg agctggagct catatttatt caaaagaaca
    13681 ttcaactaca attgtaaata tagacatagg tgggggaact agtaacatag cctcatttaa
    13741 aaggggagaa gtaaccgata ctgggtgttt ggatataggt ggaaggcttg taaagataga
    13801 taaaataagt aaaaaaataa cttatatatc acctaaaata gataaaatca taaaagataa
    13861 taatttaaat cttcaagtag gcacaatggc tactgtagaa aatacaaagc caatagtgga
    13921 tataatggtt gacttattaa tggaaagcat aggattaaag aaacagacac ctttgtttga
    13981 ttttataata accaataaat caataagtat agatgaaaat attaaaaatg tttcattctc
    14041 tggtggagta gctgattata tttactatga tggagaaata aaagattatt ttgaatatgg
    14101 tgacttggga attttacttg ggcaagcaat aaaaaattgt gaatacctta aaaaagtaaa
    14161 tgtagttaaa tctatggaaa caataagagc cacagtggtt ggagcaggtt ctcatactac
    14221 tgaagtaagt ggaagtacaa taacttacac tagcgaaaag tttccactta agaacttgcc
    14281 agttttaaaa ttatcaattg atgaagaaac aggcacttca aaacaaatca gtgaagctat
    14341 aaaaaataaa cttaattggt ttaaactaga aaatgattta cagaaagttg ctatagccat
    14401 gataggaaag aaaaatccaa cctttttaga tatacaagag tatgctaaag ggtttgtaga
    14461 tggaatgcaa caaatcatag aaaatgattt accttttata gtaattgttg aaaatgatat
    14521 ggcaaaagtg ttaggtcaag caatatatgc attattaaat tatgaaaaag aaattgtttg
    14581 tatagacagt gtcaaggttg aaaatggaga ctatatcgac attggaaaac ctatagttga
    14641 aggtaaagtt ttacctgtgg taataaaaac attagttttt aattaataga aggaggtaag
    14701 taaatgattt taaatacaaa attatttgga cgtacttatc agtttaaagg cttatatgat
    14761 gtaatggcta aagctaatga agaaaaatca ggagataaat tagcaggtct tgcagcagaa
    14821 tcagctgaag aaagaatagc agcaaaagtt gttttatcac acataaaatt ggaggatata
    14881 agaaataatc ctgcagtggc atatgaagaa gatgaagtaa caagaataat acaagatgct
    14941 gtaaatgaaa aagtttatga agaaataaaa aattggacag tctctgaact tagagaatgg
    15001 ctattggatt caaatactac aagtcttgat ataagaagaa tatcaagagg attaacttct
    15061 gaaatgatag cagcagttgc aaaattaatg tcaaatatgg acttagttta tgcagctaaa
    15121 aagataaagg ttactgctca ctgtaataca actataggag aacaaggaac attatcagca
    15181 agacttcaac ctaatcatcc aacagacgac ccagatggaa tacttgcatc acttcttgaa
    15241 ggtttaactt ttggtatagg agatgcagta cttggattaa atccagttga tgactctgtt
    15301 gagagtgtta ctaaagtatt aaaaagattt gaagaagtaa aacaaaaata tcaaatacca
    15361 actcaaacat gtgtattggc acacgttact acacaaatgg aagcaataag aaatggtgct
    15421 cctgcagatt taatattcca atctatagca ggttctgaaa aaggaaatga agcatttgga
    15481 tttaatggcg acactataga agaagctaga cagttagcct taaaacacgg tacagctaca
    15541 ggacctaacg ttatgtattt tgaaactgga caaggttcag aattatcttc agaagcacat
    15601 catggaatag accaagttac tatggaagct agatgctatg gatttgctaa aaagtatgaa
    15661 ccattcttag taaatacagt tgttgggttt ataggacctg agtacttata tgatgctaga
    15721 gaagttgtaa gagcaggact tgaagaccac tttatgggta aactacatgg cttaccaatg
    15781 ggtgttgatg tttgttacac taaccatatg aaggctgacc aaaatgatat ggaaaactta
    15841 gccatactat taacaacagc aggatgtacg tattttatgg gaatacctca tggagatgat
    15901 gttatgctta actatcaaac aacaggatac catgaaacag catcacttag agaaatgttt
    15961 ggactaacag caataaaaga atttgatgct tggatggaaa agatgggatt ctctaaagat
    16021 ggtaagttaa cagagttggc gggagacgca tctgtattat taggatagga ggtatttaat
    16081 atgaatgaga aggatttaaa agcattggta gaacaattag ttggacaaat ggttggagaa
    16141 ttagatacta atgttgtgtc tgagactgtt aaaaaggcaa ctgaagttgt agtagacaac
    16201 aatgcttgta tagatgatat aacagaagtt gatataagaa agcaattact agtaaaaaat
    16261 cctaaggatg cagaagctta tttagatatg aaagcaaaaa cacctgcaag attaggtata
    16321 ggaagagctg gtactagata taaaacagaa acagttttaa gatttagagc agaccatgct
    16381 gctgcacaag atgctgtatt ctcttatgta gatgaagaat ttataaaaga aaataatatg
    16441 tttgcagtgg aaactttatg taaagataaa gatgaatact taacaagacc agatttggga
    16501 agaaaatttt ctccagaaac tataaataat ataaaatcta aatttggtac aaatcaaaag
    16561 gttttaatat tagttggaga tgggcttagc tcagctgcaa tagaggcaaa tttaaaagac
    16621 tgtgtgccag ctataaaaca ggggctaaaa atgtatggta tagattcaag tgaaatttta
    16681 tttgttaaac attgtagagt tggtgctatg gaccacttag gtgaagaatt aggttgtgaa
    16741 gttatatgta tgttggttgg agaaagacca ggattagtta ctgctgaatc aatgtcagca
    16801 tatatagctt ataagccata tataggaatg gcagaagcaa aaagaacagt tatatcaaat
    16861 attcacaaag gtggaacaac tgcagttgaa gcaggtgctc atatagctga acttataaaa
    16921 actatgttag ataaaaaagc ttcaggaata gaccttaaat aaagtcgagg gagtgtagaa
    16981 tatgaaaaat gatgtaattc gtcctaatat actaggaatt aaaataattt caaatataag
    17041 tccagaaatg gcacaaaaac tagaattaaa atcacatcac aaaagcttag gtttaataac
    17101 tgcagactgt gatgatgtta cgtatacagc tcttgacgaa gcaactaaag cttcagaagt
    17161 tgatgttgta tatgcaaaat caatgtatgc aggtgctgca aatgcttcaa caaaacttgc
    17221 tggtgaagta ttaggaataa tagcaggtcc aagcccagca gaagtaagaa gtggtctaaa
    17281 tgcagtagtt gattttctag agtatggagc aacttttata agtgcaaatg atgatgattc
    17341 aatagcatac tatgctcact gcgtatcaag aacaggtaca tacctttcag aggtagcagg
    17401 aataagagaa ggtgaagcat tagcttattt agtagcacca ccacttgaag caatgtatgc
    17461 actagatgca gcaatgaaag ctgcagatgt taagatgtgt gaattatttg cacctccaac
    17521 tgaaacaaac tttggtggag cattacttac aggttctcaa tcggcttgta aagcagcatg
    17581 tgatgcattt gcagaagcag taaaatcagt tgcagacaat ccaacaggat tttaaggata
    17641 gttagatgga tgctataggc ttaatagaag taataggata tgttgctgca atagaagctt
    17701 ctgatgcttg tttaaaatct gcaaatgtta atattgtaag agttgataag gttggagcag
    17761 gtattgttac tttaacaata tgtggagatg tcggtgctgt aaaagctgct ttagaagcag
    17821 gtgaaatagc agcttccaga gttggtacat taagaacatc acatattata ccgagaatcc
    17881 ataatgaagt aacaaatgct ttattcaaaa ctaaagaacc taaagtctgt gaagtttcag
    17941 aagataaaga aaaaaacttt gagattacat tggaaatagc tgataatgta tcgaaaaatt
    18001 tatcagaaaa agaaaatgta attttagatg tcaatgaaaa cagtggagat actgaaaatt
    18061 tatatagtga tatagataaa acaaacaaaa aaattgaaac agttgatgat agtttaacag
    18121 aagctaatga ggaaaaaata gaaaacacta atgataaaga agtattggaa caagttaaaa
    18181 tagaaaattc cgattttgat agagatgacc tatcaaagaa cagtgtaaaa aaacttaaag
    18241 ctatggctaa gaaattagat tcttcaataa cttataaaga cttaaatgtt cttaaaaaag
    18301 aagaattaat aaacttagtt aaaaagctta gtagaaggga tagatagtta tgacactttt
    18361 agataaagat ttagtatcta tacaagaagt aagaactctt gtaagaagat ctaaagtggc
    18421 tcaagagaaa ctttctaaaa tgagtcaaga gcaaatagat aaaatagtaa aagctgttgt
    18481 agatgcagca tttaataatg ctactaaatt agctaaaatg gcaaatgaag aaactggatt
    18541 tggaaaatgg gaagataaag taattaaaaa tacttttgca tctatgggag tttatgaaac
    18601 tttaaagaac acaaaaacta ttggaataat tggtgaagat aaagaagcca atacgataga
    18661 tatagctaca ccagttggag ttgttgcagg tcttatacct tcaacaaacc caacatcaac
    18721 agtaatatac aaaactataa tatctctaaa agcaggaaat actatagttt tctctccaca
    18781 tccaaatgca aaaaactgca tacttgaaac agttaatata ataagtgatg ctgctataaa
    18841 ggctggagct ccagaggatt caatattatg tataactaat ccaagtatag ttggaaccaa
    18901 tgaattgatg aaacataaag atacaaactt aatactagca actggaggag aagcaatggt
    18961 tagagctgct tactcttcag gaacaccagc tattggagtt ggaccaggaa atggaccagc
    19021 atttatagat aaatcagcaa atgttaaatt agcaatcaaa agaataatag attctaaaac
    19081 ttttgataat ggtgttatat gtgcatcaga acaatctata gtaatagaaa aatctatgga
    19141 agaaactgtt ttaaaagagc ttaaaaatca aggtgcatat gttttaaatg aggaggaaag
    19201 tgaaagatta gcaaaattca tattaagagc taatggtact atgaatccac aaatagttgg
    19261 taaaagtgtt gaggtaatag caaatttagc agacttaaca aatgtaccta aaaatacaaa
    19321 agttctagtt gcaagagaaa ataaagttgg tcacaatgta gcctattcaa gagaaaaact
    19381 tacaccaata ctagcgttct atgttgaaga aaatgtagaa gctattatga acagatgcag
    19441 agaaatattg ttaaatgaag gtgcaggtca tacattctca atgcatgtaa atgatgaaga
    19501 attggtaaaa agatttgccc ttaatatgcc agtatcaaga atacttgtga atactccttc
    19561 agcacttgga ggaataggtg catcaacaaa ccttgtacca gcactaacac ttggttgtgg
    19621 tgcaataggt ggaagttcaa cttcaaacaa tataggacca cttgatttaa taaatgttaa
    19681 aagagtagct tatggaatca aagaaataga agaacttgga agagataaat ctcaagttga
    19741 aaaatcaatg tttgactcaa tggataaaga agaattaata aatcttcttg ttcaaaaact
    19801 agtcaaagaa ctagcataac taaaataaac aatgaaactt atggctatcg ctataaattt
    19861 ctaaaaatat aaactataaa aaactataaa attaaatttt tggaggataa gattatggca
    19921 agtgcaaacg cattaggaat gatagaaact aaaggattag taggagcaat agaagcagca
    19981 gatgcaatgg ttaaagctgc aaatgtacaa ttagtaggaa aagagcaagt aggtggagga
    20041 ttagttactg taatggtaag aggagatgtt ggagctgtta aagctgctac agatgcagga
    20101 gctgccgctg ccgagagagt tggagaacta atatcagttc atgttatacc aagaccacac
    20161 tttgaagtag atgctatatt accaaaggta tcagctgagt aatataagaa taattataaa
    20221 atttaaattt tgattgaaaa agaggctatc tcaaaaatga gtcttactaa taaactcaat
    20281 gtattttgag gtagtctttt ttatacatct aataggagat gtgataaaat gggtgtttta
    20341 acagaaaacg aagtaaaaag aaaaattgga aaaaacaaaa ataatataac tgaattttat
    20401 atagaaaaag gtcaaataat aactccttct gcaaaatctt atttagctga gagaagtatt
    20461 gagcttaaat atgttgataa actttcagat gtaaacaatg aacctaaaga agttgtaaaa
    20521 gaagtagtta aagagaaaaa atatagatat actactgtat ttggcgctaa attggatgaa
    20581 aaaccagaac atatgactca attaaatgga aatcttttag tttttaaaga ccataaaaga
    20641 ataatattga gaggaaaaat agattctctt gaatcagaaa tactaaaggt acagatactt
    20701 tgtcagaaac tagatatgcc aaagcttgtt aaggaattgc aagaagtatt agactttgta
    20761 agaaatataa tgagatgtga agttttagat gaaaagctag gggactttaa acttttagga
    20821 ttaactcctc aagaacttag agaacaatct cactatccta aaaaatactt tggtatagga
    20881 catgagttcc caagttatga aatgggagaa gtagtagtag ctataaatag tataagaagt
    20941 aatacacgag aggttgaact tgcagcctat gaagcattta agggtgagta tggaaatgtt
    21001 cacagagaag atttaataaa ggcacttaat agattatcgt cagtattttg gataatgata
    21061 tataaggtta gaactaataa gtataagtag ttggaggaca taatggagaa tctattaaat
    21121 gaaatagtgg aagaagtaat aactagagtt aaaaaagaag catttataga agttgaagca
    21181 tcaggaagac acgttcactt aagtagagaa gatgtcgata aattatttgg agaaggatat
    21241 actcttacaa aactaaaaga cctatctcaa ccaggtcaat atgcatgtaa ggaaagagtt
    21301 acaataactg gtcctaaagg aagtataaaa aatgtagttg tacttggacc atgtagaaat
    21361 gaaactcagg tagaaatatc tttaacagat ggttcaactc ttggactaaa agctcctata
    21421 aaacaaagtg gagacttaga aggtacttta agtattaaaa tatcaacaca atatggtgaa
    21481 gttgaacttg ataaggggtt aatggttgct aaaagacata tacacatgac tcctaaagat
    21541 gctgaaaaat tcaatgtatg tgataaagag atagttgaag ctaaggtgat gggtcaaaga
    21601 cctttaacat ttgatgatgt agtaataaga gtgagtgaca gttttaaaac ttatatgcat
    21661 atagactatg atgaagccaa tgcatgtggt tattcaaaag gaactgttgc taaaataata
    21721 aagaaagcgt aaatttaacc tactatataa attacctcta gggaggattt aatatgaatt
    21781 atgataacct tgtagattta ataacggaag aaatctataa aaaaataaat agtaatgaaa
    21841 taaaaatatc taataaacct aaagctgtga ttgtatttga acaagataac aataaattta
    21901 acttattaaa agatgaattt gaaatagtag aatttgataa aagtataagg gaatgtgaaa
    21961 tagtgatagt ttcaagactt tgtatgaggg ggctatcgaa tatagcactt ggaaattcaa
    22021 caagtgatga agaaagattt atactaaaaa tgattatgaa gggcaaagaa gtatatgtcc
    22081 tagatgaagg aattgaatac aaaaaatata aggatactgc accaaaagca ttatataaga
    22141 aatttatgtc atttgaagat gaaatatgta aatttggtgt agaaattata aaagacttaa
    22201 atgttataac taaaaataag ttaaatataa agaaagaact agaaagtact agaggttcta
    22261 aaaaagaaga tacaaaagtt ttagataaag atgttttaaa tttaaccagt gtagaaagtg
    22321 taaaaataga taatgaattt agtttagatt taaggaataa aaaacttata tcggaagctg
    22381 acttaagaaa accaactata aatggagtta aaaacatatt agttaataaa aaaagtataa
    22441 taacaccatt agctgtagat tttatgagaa tacaccatct caaactaaag aaagtgtaag
    22501 gaggtaattt tatggatata ggaaaagttg taggtaatgt ttgggctaca agaaaagatg
    22561 aaaaattatg tggccaaaaa cttttagttg taaaaatttt aaaaactaaa gacacataca
    22621 aggaaggctt catggttgca gccgataatg caggagcagg aaatggagac ttagttctta
    22681 tatccaaagg ttcctcagct agagagtcga ttgaaaaatc tcatgttccg gtagatgcaa
    22741 ctatagttgg agtagtcgat tcgtttgagg tgtttgatga ataaaagtat aggagctata
    22801 gagtttaaaa gcattgctaa aggtatagaa gtatccaatg aaatgataaa gaaatcttca
    22861 gttgatgtat tgtacttaaa aagtatttgc ccaggtaaat ttctaataat agtaggtgga
    22921 gaaacttctt atataaatga atgtgtagat tatggaataa aacttggaga aggatatatt
    22981 gtagataact tcgtaataaa tgcaatttca caagaaatat tagatggatt taaaaataag
    23041 tatcaaaagc tagatagtat agtctctata ggcgttgttg aaagtagtaa agtatgcgct
    23101 ggaataaaaa tgcttgataa gactttaaaa tctggcgact tagtattagt aaaacttcaa
    23161 ttatcttttg ctataggtgg caaattagta tatatagtag caggagattt aagtagttta
    23221 gaatatgcat taaaagaaag tgaaaatgta gtaagagaaa aagaagtaat atataagact
    23281 gtaataccat cagtagacag tcaaataata aaaagtctaa taaaataatg gggggaatta
    23341 tgagtataaa cgaaattata atctatgtaa tggtagtctt catggtactt ggagctatag
    23401 ataaatgtat aggtaacaaa tttggtcttg gagaacaatt tgaagaaggt ataatggcaa
    23461 tggggtcact tgcagttgca atggttggtg ttatatgttt agcaccagtt cttgctgatg
    23521 tattaagacc aattgtagta ccagtattta atatacttgg agctgaccca gctatgtttg
    23581 ctggaagttt attagctaat gatatgggtg gtgcaccact tgcacttgaa cttgcgaaag
    23641 accctaatgc tgggttattt ggtggattaa tagtaggggc tatgatggga cctacaatag
    23701 tatttataat accagttgcg cttggaataa ttgaaaaaga agaccaaaaa ttcttagcaa
    23761 caggaatact tgcaggtata ataacaatac ctattggagc attcgttggt ggattagttg
    23821 caggattcga ggtaatgatg gttcttagaa acttagtgcc aataataata tttgcagtag
    23881 ttatagcact tggattatta aaattcgaaa atgctatgat aaaaggattc acatactttg
    23941 gaaaaggtgt agtaatagtt ataactttag gacttgctgc tgccatagtt gaagctttaa
    24001 caggaatagt tgtaatacct ggaatgaaac caatcagtga aggtatagaa atagttggag
    24061 atatagcaat agttttagca ggagcattcc cattagtgtt tgttataaca aaggtattta
    24121 ataaaccatt aatggcttta ggaaaagttc ttggaatgaa tgatgtatca gcagcaggcc
    24181 ttgttgcaag tttagctaac tgtataccaa tgtttggtat gatgaaagat atggacaata
    24241 gaggaaaaat aataaatgta gcattctctg tatcagcagc attcgtattt ggagaccact
    24301 taggatttac ggcaggattt aattctaata tgataacacc aatgatagtt gctaaattag
    24361 ttggtggtat aacagcagta ttgttagcta tggttatcgc aaataagaca ctaaagaagg
    24421 agaatgtata agatggatat atcaaatata gataaaaatt taattgaaac attagtaaga
    24481 caaataatag aagaaaaaat aagtggaact aaagacacag tagattttgt tagaaataaa
    24541 gacattagtg gaataacttc tataaagtta ccaactgtaa aagttagtga atctgataga
    24601 ttagatacag ggaatccaag tgatgtagtt tacacaaaag atttatttac attagaggaa
    24661 agcccaagat taggttgtgg tatgatggaa atgaaagaaa caacttttga ttggactcta
    24721 aactatgatg aaatagatta tgtaatagat ggaactttag atataataat agatggaaga
    24781 aaggtttctg cttcttcagg tgaattaata tttataccaa aaggaagtaa gatacaattt
    24841 tctgtacctg attatgctag atttatatat gtaacatatc cggcagattg ggcatctcaa
    24901 aattaataaa aatcaagtta agttaataat aatcaaataa ataatttaaa atacaaactc
    24961 ttaaataagc acacatattt aaaaaagcta tgaattaaaa ttcatagctt tttttataaa
    25021 gaatgttata ttttagacgt attttttcta tatttactgg gagacatatt atatttttta
    25081 ttaaataatc taataaaatg ctctacattt ggataaccaa tactatttta ctgataatct
    25141 tttccttatc actaatctta cattttaaaa tattattttt actcgtaata ttatcataag
    25201 aatttgtttc ttatttaaaa ctaattagaa ttataaagtt tattgccatg ttattaataa
    25261 aataaatcca gttaaatcga gtgctagtta aagtaataag aaacttttac aatggtttcc
    25321 tttatcgatt gaaataaaag ttaatatggt ataatatcta aagagagaat cttatcaatt
    25381 agaatatgca tagaaacaag tggcttaaaa ggagacgtta tatggaagag atagtaaaga
    25441 aggaacaagc aagtatgaat gatatttttt tgtatcttaa agctaaatca aactcattat
    25501 ataaatttgt aatattatat aatgagttta tcaataaacc taaagattat ggaactgggg
    25561 aacttataaa catggttgaa gtacatatgc taacagcgat tgaagaaaat ccaggaataa
    25621 gtccaactca gttagctaaa atatggaaca gaactaaagg tgcaatatct caaactatat
    25681 ctaaattgga gaaaaagggt tatataacta aagaaaagaa agaaggaaat gcaaaaacta
    25741 tattactata tccaaccaca tcaggagtag agttaagtaa ggcacataaa ctatatgaca
    25801 ccatagatgt tacaagaaca tttgaatact taatcaagga gtgtacatta gaggagatag
    25861 atagttttta taaagtaatt gatttgttta ttaatttatt agaagaatct ccgcaggaaa
    25921 ataatgattc tgaaaaagtg tagctgagat tgaagttgaa taaagagaaa tagatattat
    25981 agaagtagta cttaagtaca cattatataa aaatataata ggtataggat taaaaagttt
    26041 agtggtttaa agtttaacca ctaaactttt ttaatgtcta taatctacta ttatttaatt
    26101 tactaaaaat aaaaaaactc attatattaa gataaaaata gaaaaataac tagaaaaata
    26161 ctataatata tcagcttaat acagaaaaaa gttaaaaaat ttgagaaaaa taaaaatgaa
    26221 aacgttttat attgacatag agaaaaagta gtgataccat ttaaatatag tttagtagct
    26281 aaactatatt taaattataa taactatttt agggggatgt tatgaattta aaacaattaa
    26341 gtgaaaaata cgaagaatat gtaattcaaa tgagaagaga atttcataag tatccagaat
    26401 tatcttttaa agaaataaga acaacccaaa gaataaggga agaattacaa aaaatagggg
    26461 tcccttttgt atctattgaa ccaaatatcg taatagctac tatacaaggc aaaaatgaag
    26521 gtaagacaat tgcaattaga ggagatatag atgcgcttcc aatgcaagaa gattcagatg
    26581 ttgaatacaa atcagaatat gatggaataa tgcattcttg tggacatgat gctcatggag
    26641 ctatgttact aggacttgca catatgctaa atgatataag aaaagattta aaaggtagag
    26701 tattattagt ttttcaagca gctgaagaag ttggtggtgg acacaaggag ataatacagt
    26761 actttaagga aaatggagga ccagatagat taatagctac tcatgtttgg tccgatattg
    26821 aagctggtaa aatttctgtt gaaccaggac ctcgtatggc tggtgggtct ggttggagaa
    26881 tagacataac aggtagagga ggtcatggtt ctagaccaga tcaaagcata gatcctataa
    26941 agccagcttg tgatatagtt ttaaaagtat cttctatacc agttaatcat ataagtgtac
    27001 tagaacaatg tgttgtacat gcatgtgcaa tacatggagg aactacaata ggaaatgtta
    27061 ttccaaacaa tgcagaggta ataggtggat atagatattt tactgaagaa tcaggcaaaa
    27121 aagtgtttga cattataaag caaatgagta atggtgttgg acaagtttat ggagtggatg
    27181 tgaaggttac tcatactgga ggcataggac ctgttatcaa tgatgaagaa gcagttaaga
    27241 tggctaaaga aatagtttct aatatagatg gattggagtt agattcttat gagccaatat
    27301 gtgcatcaga ttgttatggt gaaatattaa aagagtattc tggcttttat tgttatctag
    27361 gggtaggtaa caaagaaaaa ggaataatat atgcacaaca ccatcctaag tacaatatag
    27421 atgaagatac attaaagtta ggttgcgaat ttatggctaa atatgcagtt gaatttttaa
    27481 ataaaaataa taactaaaat aaatcgaatt ttataaatgg gggggatgaa aatgcaaggt
    27541 gcattaaagg aaaataagaa aaaaagtggt ggaggatttt taggatggat tgaaaaagtt
    27601 gggaataaaa tgccacatcc tctagcatta tttacatata ttacattgat agtagtagca
    27661 ttatctgcaa tagcaagctt tttaggtttt tcagcaacaa gtcctgctac aggaaagtta
    27721 ataaaagtta tgaacttaat aagtgcaaaa ggtttagtac tatttatgca agagtttgtt
    27781 aagaacttcc aaaacttccc tgtgcttgga gtagttttag ttatggcagt tgccacagga
    27841 gtttgtgaaa agacaggatt tttttcaact gccataaaaa tgagtttatc aaaagttaag
    27901 gggaatgcgg ttgtatttat aatagctttt ataggagtat ttgctaatca agctggtgat
    27961 gctgcttttg tattagtacc aactcttgca ggagcaatat tttatggatt aaatagaaat
    28021 ccattagctg gtatattctg tggatttgca tcagttggtg gtggatttgc aacagctgta
    28081 attcctgggg gatgggacgt tacgctaacg ccaataactg tatcagctgc tcaaactatt
    28141 caacctgcat ttgatatgac attattagct agttattatg cactatttgt atctgcgttt
    28201 atagtagcta cagtatcaac aatagtaacg gttaaattta tagaacctaa attaggtaag
    28261 tatactggta aaccagaagg tatagatgat aatcaaggtt ttgaggtcac aaaagaacaa
    28321 gcaaaagcag caaagttagc aggttttaca gttttagctt atgtagcgtt attaattgct
    28381 ttgtgtatac cagctaatag ttttttaaga agtccagaag gttctttaat atttggagca
    28441 ccacttatgt cttgcttaca gttttttata gtaatactat tcttcttgcc agggattgtg
    28501 tatggaatga gggttggtaa aatcaagact gtaaaagact taaatgatat attatgtcaa
    28561 tcaatggcag caatggcacc gtttatcgta cttgcaatag ttataggtca attcttaacg
    28621 ctattctcag tgtctaattt agcacaggta ctagctataa aaggtggagc attactcaac
    28681 tcattgccac taccaccaca agctataata ctgctattct tagtactagt agcgtttata
    28741 aatatatttg ttataagcgg aagtacaaaa tggatgatat ttggaccagt atttgttcca
    28801 atgttaatgc aattaaacat acatccagca tttactcagt ttgtatatag attaggagat
    28861 tctgtaacca atcatttatc accattaaat gcattcttta taatactttt agcaacagtc
    28921 caaaaatacg ataaaaatgc aggtatggga actatattct cagctatgat accttacact
    28981 ataggttact ttgtagtact tgcagtttta gtagtagttt ggatgacaat aggtatacca
    29041 gtaggaatag gtggacaagt ttggttataa aacatattta ataattttgg gaggaaagaa
    29101 aatggaaaca actgaaataa gatacaattt tgatgaagta atagatagaa gtggaacaaa
    29161 ctgtcttaaa tatgatctta agagtcaatt tatgccagaa tctccagaag atgcactacc
    29221 actatgggta gcagatatgg attttgcatg tgcccctgag ataattaatg caatgcatga
    29281 aagaatagac agaaaaatat ttggatatag ctctcaacaa actgatgatt attatgaagc
    29341 tgtatgtgga tggtttaaga gaagatttga ttggaaaata gaaaaagaga atatattctt
    29401 tagcccaggg gttgtgcctg cgttaggatt tttcacacaa atattaacac aaaagggtga
    29461 tggtatcata atagaaaagc ctgtatacta tccttttgaa gataaaataa ctaataatga
    29521 aagaaaagta gttaatagtc ctttaaagtt tgaagatggt cattattcta tggattatga
    29581 tggatttgaa gaattagcta aagatgaaaa taataaggtg tttatattat gtagtcctca
    29641 taatccagtt ggtagagtat ggaaaaaaga agagcttaga aaaatagtag acatatgtaa
    29701 gaaatatgat ttgtggataa tatctgatga aatacattgt gatattattc gtaaaggtga
    29761 aaagcatata ccattagaag tagcatgtcc tgattataaa gatagaataa taacttgtac
    29821 agctccaagt aagagtttta acttagctgg tatgcaatta tcaaatatag taatcaataa
    29881 taaggaatta caaaaaaaat ggatgcttga aacagattct aaattagcta tattcctacc
    29941 taatccattc gcaatagttg ctactaaagt tgcttataat gattgtgaag attggatgaa
    30001 tcaagtaaat gagtatatag atggtaatat taaatatgta gaagattttg ttaatgagta
    30061 tatgcctaaa gtaaagataa taaaatctca aggaacttac ttagtttggt tagactttag
    30121 agggtatgga ttagatgcaa aacagcttga atatgtaatg cttaacaagg cgaaggtatt
    30181 gctagatgaa ggatacatat tcggagaaga aggagcagga tttgaaagaa taaatgttgc
    30241 ttcacctaga acaattatag aagagtgtat gaatagaata aaatgtgcat ttgaaggatt
    30301 ataatagtaa tatctaaata atatatttgt gtattttaaa aaaagactat gctaaaaaaa
    30361 tatttcaaag catagtcttt taaagtttta tgatgagttt aaaaaatttc aaaacgttac
    30421 tattcactta tatgtttata ttagaaataa ttccacaata tactcatttt ctaaagttgc
    30481 acttttatac accttgcttc accaatatca tgtgtaacca aaatagtaga cttattctca
    30541 ttcttaataa tactataaac atcatcacaa actaataatc tagtctgata atcaagagct
    30601 gaaaaaggtt catctaaaag tagaatatct ggatttacag aaagtgttct aataagcgca
    30661 actctttgtc tcatacctcc agataattct tttggataca tacttctaaa atcccataat
    30721 ccataagtct ttaaaaggct ttcaactcta tctaaagatt ttttatcttt tttgccttga
    30781 atttccaatc caatagttat attatccatt atatttctcc attcaagcaa attatctttt
    30841 tgaaacatat atccaatatt tccagtaatc tttatatcac cactagtcgg ttttaaaaga
    30901 ttagtaagaa tatttaatat tgtagattta ccacttccag aaggaccaag caaagttaat
    30961 atttctcctt ctttcaagtt aaaattgaca tcctttaata cttcaagctc gccttcctta
    31021 gtgtagaagt tcatatttat atttttaact tctactatgt tgtcattcat tatgccaccc
    31081 cctataactt ttagctcgta tatattatgt tattaacaat atgacctatt tgtaccattt
    31141 ttcataaata taatataaat attttgttta tttacgtatt ttcttagtaa cttgtacgta
    31201 taaattatag ataaataaat tataaggtag gggataaatt tgagtaacat aataacgaat
    31261 ttaatagagt caatatttac taatccaata actatattgt ttttacttgc aataactgtg
    31321 tattctgttt ttaaaataat agaaaataac agagtatatt catttgtaag ttcaaaatta
    31381 gacgaaatca atagagacta taagacaagt gagttttata aaaaggtaag ggatgactac
    31441 tatacatata gtaaagaaaa tccttatgca gacgtaaata taacttcatt tatagaggaa
    31501 gttgtatctg atttaaagca taataatttg ccattgttag aaaaaattag aagtataaaa
    31561 aattcatctt caataagtat attacttggg gttttaggta cgtttgttgg tctttctaca
    31621 atgcttttat gtgttgatac taaagacatt ataaattcac ttccttcaac tattagtagt
    31681 atgcaaactg catttacaac tagtatattt ggagttgtat tttctctaat tataggttac
    31741 tttactaaga taaaagattg tgaacatgtt ttgatacaaa taatgttaaa atctgaaaat
    31801 ttactaacat ctgaaataac tcattttaag tcagaaagaa tggatttaaa agtagaagag
    31861 gtaaaaaata caataaaaca aataagtaag tctatagaag ctattgaaaa atttgataaa
    31921 atatctaaag atttaaatga ctttaatgat gaatttatca gtggtataga agctttaaaa
    31981 agccttttag aaggttctca aagttctatt aagacatttg accagagcgt aagaaaatta
    32041 gataaacagt ttaatatttt aaatataaaa tttgtaaagc tttttgataa atatgataat
    32101 caagataata taaataaaga gattttattt gatataaaag aatcttcaaa aaatatatac
    32161 aatgccactg aaagccaatt caaaattaga gattatataa aaaatataaa tgcaggattt
    32221 gctctatatg aaagaagtgc tcaagattta ttaactaaat taatgaccca tgaaaataaa
    32281 atatctcaaa atcaaaaaat ccttttagat gaaaagttta ccttagatga ctcaataaaa
    32341 aatctctcaa gtattattga aaacttttct aatgacctac aagttaaact ggatatgatg
    32401 tttgaaaatt ctttagatat tcaagataag ttagatgtta tgtttaataa ctcttttatg
    32461 aatgatgaag ttcctcttga tagtgaagaa ttatttaagg atgatgttaa taatgtattt
    32521 aatcctttta gtgaagaaat ttatgagata gaggataaag aaataaaggt cataggtgag
    32581 gatgagttaa atgaataata aatatagaag aactgttaac agagaatttg aaaagagtag
    32641 cttttggcca acatttacag atttattatc tactgtattg atggttgtga ttcttatctt
    32701 atttagctca gaaagtattt caggttctgt tgaacaagat ttagctaaga atgtcaatgc
    32761 ttctgttgaa gaaactttta aaaaaagtgg aatacctgtg aaggtagata aatctaatgg
    32821 gcaagtaact tttggtgaaa aaaccatgtt tgatgtagat agtgatgttt taaaaccaga
    32881 ggctaaagaa atgctaaaaa tgtttgttcc aaagtacata gagactattt ataaagatta
    32941 tggtgactat atatccaaaa tagttataga aggtcataca gatgatgtgg gaagttatat
    33001 atacaactta gatttatcgc aaagaagagc atatagcgtt gctaaattta tagtgggaga
    33061 tgaaataggt gactataaat acaaagataa agtgactaag catattattg caataggaag
    33121 gtcaaaagct gaactcatca aaaatggtga caatagcgtg aatagagatg catctagaag
    33181 agtagaacta aaatacgaaa taaatattaa tcaaaataaa taattaaatt tttataaaaa
    33241 aaaagaaggc agttggtgtg agctttaagc cttctttaag gaaaaagttt atttattttt
    33301 gttgatactt aacatattat agtttttaga agcagtttgc aatatattaa acgtaaatgg
    33361 tcgtttaaaa tgcataactg gatactactg atgtaaaata tttcatttta tataatataa
    33421 aaattataca gtgtcaattt tctatattat tgtactataa tctattattt aaaatattat
    33481 gtaaaaaaat atcataaaaa cgcatatcat gttataatta ctagagtatt atgaatatgg
    33541 aggcgtagtt tgtgatagaa ataagtataa aaaataaaaa aaattctaaa attaaaactt
    33601 tgatactttt attgatagta tctatatcga tttatttaac tggttgtact acatcagata
    33661 atggaaataa tcaatcaaat aataatgatt ctttgtctag tagtatatca agtgataaga
    33721 atgtaaaaat tcattttatt gacactggaa atagtgatgc aatattgatt caagatggta
    33781 aaacttttac attgatagat gggggagata atgatgatga gggattaatg gtagattacc
    33841 taaacaatca aggtgtaaaa gatattaaat atcttatagc tacccattct catgcagacc
    33901 atttaggtgg acttgattct gttgtcaaaa actttaatat agaaaatgtt tttgtatcga
    33961 atggttcagc agaaacaaag agttataggg actttataaa tgctttagca aataagggtt
    34021 tatccccaag tgtaccgctt gaaaataata agttctattt agaagattct tattttgaag
    34081 ttctaaatac aaatggggga gatactacta atgaacaatc tctagtcctt gtatatacta
    34141 atgggaatga taaagtttta tttacaggtg atgctgaaga aggtaccgaa aaagaaatac
    34201 tacctaagct tgagaaagta gaccttttaa aagtagctca tcatggttct cgtagtagtt
    34261 catctcaaga gtttttagat aaagtaaatc ctgaatatgc ggttctttta gtaggcaagg
    34321 gtaattcata tggtcatcca catcaggaga caatgaataa attaaaaaaa atgggagtaa
    34381 aagtgcatag aagtgatgag tgttcagata taatatttga atcaacaggt gatggagtat
    34441 ttacaagttg taaagatgga agttataata aaggcgttag agaagatgga aactctggaa
    34501 aatcaaacag taatacaagt aaaaatgatt cttttaaaaa taaacaaaat acatccaatt
    34561 cagaagaaat agtgtatttt actccaaaag gtaagagcta tcactctaca aaaaattgta
    34621 gtgggctttc aagaagtaaa aaaatattat caggcacaat tgcagaaagt aagaaaaatg
    34681 acccatgtga tatatgttat ggaaaataac aaaaaaacaa ccaatttatc tataaagctt
    34741 ctgaacttta aatcatttaa aaaataaaat aaatgcataa aaaaataagc tataatcaat
    34801 agataacaca ttttatctat acgaatagct tattttttta agtatttttt aatagctatt
    34861 taataactct aaatacacca gttacaatac ctacaatttc aacttctgag tcattgagca
    34921 taattggttc cataaaatca ttttctggct gaagtcttat taaatttcct tctttaaaaa
    34981 acctcttaac cgtagcagat tcaccattta taagtgcaac tactatctga gaatttaagg
    35041 ctgtattttt tttgtctact acaacgtaat cttcatctaa aattcctgca ttaatcatac
    35101 tttcgccttt tactcttaat acaaaattgt tactgccctt tactaaactg gcagggaatg
    35161 gaatgtattc ctctatgttt tgttgagcta aaatgggttc tccagctgtt atttgaccta
    35221 ctaaaggaag ctctattatt tcttgattag ctccagaaac atcatttact ttactacgtt
    35281 ctaaaacttc aatagctctt ggtttagtag ggtcctttct tatgtatccc agtttttcaa
    35341 gtttatttag atgagagtgc acagttgaag tagatcttaa ccctacagca gtacatattt
    35401 ctcttacagc aggtggataa ccttttaata tgatttgaga ctttataaat tccaatatca
    35461 agacttgctt ttcagttaga tctaaataca taacatcact catttccaaa catattttgt
    35521 ttttatttcc atacacttta tatttttaat tataacataa tccatttata tctgcaaaca
    35581 tttgttcctg atttttaatc gtcctcaatc cattcaaaat tcttgactat atcacgtttt
    35641 acatttttct tatgagctgc ataaagctga gtagttgtga cattatcatg gtcaagtaag
    35701 ttttgaacat catatataga aaatcctgtt tggattagag aagttgcagc cgttgctctt
    35761 aatttatggg gactatatcc attgtctcta gtagtatcaa gtggaataga tgtatatttt
    35821 tttaccagtg ttcttatagc ctttgctgta attctcttat tttgaagaga caaaaataaa
    35881 gcatcttttt cttcatcatt taatagttca tctcttgttc tttcgttttg caaataatct
    35941 tttattacat gttcacaagt atgatttata ggcattaaaa cttctttccc acgttttcta
    36001 tatattttaa attcacctct tgaaaaatta aaagaggaga tattgagttc tctaagctca
    36061 tttaatctaa gcccgtatgt aacaaataat gctagtatag ctttgtcacg taatttagtt
    36121 tttctccaat ataccttttc tttctctgta agcccttcac ctgtttctac tgattcaagc
    36181 atcttagcaa cttcatctat ctcaaggcgc ttaatagcat ctggctgagg tttaggaagt
    36241 tttattggat tgaatccatc agttatatta ttatctatct gagagtttct gtataagaat
    36301 ttaaatagag tagagatgga tgattttttt cttgctaaag ctctattgtt attttcaaaa
    36361 atcaatgtgt ttttttctgt atttttgtaa tatctactgc aataatcacc taagaataaa
    36421 tttatatctc ttgattttat tgtattaaaa tcatcttctg taatgtcttt tacacaatct
    36481 gcatttgtta attctttggt ttcaatcaaa tatgaacaaa aaaaattaat atcttctaaa
    36541 taagcaagtc gtgtagaaac tgcaacagaa ccttttaaat atataaaata atccttcata
    36601 aatacaggaa gtttattttc caaaagtaga catttttcta tgagttcatt ttctttgata
    36661 acaatattat caggtttatc tgatttatta tgtactttta taattttctt atcattattc
    36721 aaaattaaac ctcctaaaac taaaatataa atcttttata ttattttttg atatttatac
    36781 taagtgagta taaatattta atttaatcat tttttacatt tttaagagat tagcagtgct
    36841 ttagatgaaa ttatcactaa ctattcccta aatccacatt taaagactag ttgaatttaa
    36901 gtactctttt ttatttctca atatatttat ataaatatat tgagaaattt tttatgctct
    36961 actagaatct tttttatata atatttttat atgtttctaa cgaaaccaaa tattagtaaa
    37021 atatgtattt atttaactaa agtttttaaa tgggctgact aagcttaaaa tataggatat
    37081 tatgagagtt actattgaaa aaataaaccc tattgccata aagaaactta taaatatgtt
    37141 atacttaact attaaactgg atatattcaa gttatcaaat ccaaataagt atgtaattga
    37201 acttattaca aaaataacta taactataaa gctcaggcca tttaagctat tttttaaatc
    37261 agcactacta agtgacatat gtgtagatat agaatacatt aaaaacataa atataaaata
    37321 cttcaatgaa tatacactag gtgttaacaa cattattctt ataaaattgg aaatatcacc
    37381 taaaataatg tgtataaaac catttatatt taaattatta atcatgctta catataactt
    37441 taaatctata ttttgagtca ctttcatata actatctggc agtaatatct taaaaattaa
    37501 aataatcatt attgttccac ctataatagg tgcaattcct ataaagaaat ttcctatact
    37561 ttgataaaga ttatcttttt tatagctatg acttacatat cctagtatac catcattttt
    37621 actttctatt ggtctgaaaa acttaactga atttatttta tgcatgaaaa ttaaacacat
    37681 tatcaaatga ctcaattcat gaacaaatgt tcctatagct ccagttacaa taattccagt
    37741 ttttccaaaa gatgaaaata taaatttatt atttagattt tctgtaatgc taaaaatcaa
    37801 tccaaataca ataaatattc ctatgaaaca aaaaatattt attacactta tttttaaaat
    37861 ttcgattata taaatcataa gttaaatcct catgtaaaag tatttaacta ctctacttca
    37921 tatccattac agcttaaaat tttacaagca ttttctatat ccttttcttt tactaagata
    37981 tagtctgtat cataagtaga aattgcaaat atgcttattt tgttgtctgc taacaaacta
    38041 cttaacttag ataaaattcc tacaagtgaa aagtctaaag gacctagtat cttaagtatt
    38101 ctccactcct tttctgactt aacatcagaa ggaatattat tatttaaaca tacaacactt
    38161 aattcatcat cagttctagt tatagagtaa aacttctctg tatctatcca tgttggtatt
    38221 ttactgtcat tatttaatct acatactgca tactcttcta ccaataattt taatttcata
    38281 atgtatattt cctccaatct tatttgttta cttaagatta tacattaaaa gttacataaa
    38341 ataaaacata taaagtaaaa aaggttcact atagtgtcct atttctatag ccaaccttta
    38401 aaacttaatt aaattctaat aaagagatat attatctagg ttcaattata agtttaatgg
    38461 ctgttttctt atcaccttcg atttctatat cagtaaatgc gggaatgcaa actaaatcca
    38521 ttccactagg tgcaacaaaa cctcttgcta ttgcaattga ttttatcgct tgatttagag
    38581 ctccagctcc tatagcttgt atttctgcaa cgcctcgttc tcttagtact ccagccaagg
    38641 ctccagctac agaattagga ttagattttg atgaaacttt tagtacctcc atttcaacat
    38701 ctccttgtta atttgttaaa gtatccatta cctaaagtac taaatcataa ttgccattta
    38761 ctaattacaa tcatcattta aagtatactt ttataaaaat atacttctat aatcgtttta
    38821 agaaaacctt tatttttttt aaccatttta ttatgaaaaa actacaaatt tctttatatt
    38881 ctataagcat tttccaatat ttctaaattt tttatatctt tgagctataa tttcctcttt
    38941 atccatatct ttgtaatgat ttaatctttc taaaatgtaa gcttttattt cttttgaaac
    39001 agcttctaca tctttttgtg ctcctccaag aggttctttt ataatcttat ctattatttt
    39061 taaatcatat aaatcattac ttgtaagctt cataacatca caagcttctt tagctttaga
    39121 tgaatcttta aatagaatac tagaaagccc ttctggagaa atcacagaat aaactgaatg
    39181 ttctaacata catacatcat ttcctactcc gatacctaaa gctcctccac taccaccttc
    39241 accaattaca aaagtgatta ctggaacaga aagtttactc atttcaagta agtttcttgc
    39301 aatagcttct ccttgtcctc tttcttctgc ttctagacca caaaaagctc ctgatgtatc
    39361 aataaacgtt attataggtc tattaaattt ttcagcttgt ttcataagtc ttaatgcttt
    39421 tctgtatcct tctggatgag gcattccaaa atttcttttt atattttctt ttgtatctct
    39481 tcctttttgg tgtccaacta tagtaacatt aaactttcct atctgaccaa ttcctccaac
    39541 catagaaggg tcatctttaa aaagcctatc tccatgaagt tctaaaaaat tagaacaaat
    39601 atgttctatg tattccaatg tcgtaggtct ctttatatct ctacttaatg ttaccttttc
    39661 atatgctgat agatgtgaaa aagcatcttc cttaagcttt tccaatttct cattaagtga
    39721 atttatttta tcacttaaat ctatattatt tgccttagaa atagttatta attgtttaat
    39781 atcattttct aagactttta ttttttcact attattttca atcaaaacag ttcctccttt
    39841 atgccctatt tcttgtgcat agcaagaatt tgatataata cttccttcat ttttcttcta
    39901 tctactatca tgtcaataaa acctttttca agtaaaaact cagaagtttg aaatccttca
    39961 ggaagttttt gatttattgt ttgctctatt actcttggac ctgcaaaacc tatcaacgta
    40021 tttggctcag aaattataat atcaccaagc atagcaaaac tagcagttac tcctccagta
    40081 gtagggtcag ttagaactga tatatatggc aatgattttt cttcaagctt agaaagtgct
    40141 tgagaagtct ttgccatctg cataagagat accatacctt cttgcattct agctcctcct
    40201 gaagcagaac aaataattat aggtaaatta ttttcagcag catactctat tgaataagtt
    40261 attttttccc caacaataga acccatactt cccatcataa aatcaggatt cattacacaa
    40321 ataactgttt taattccatt tattcttcca taacctgtga caacagcttc tgactctttt
    40381 gttttttcct tataagagtc tactttttct ttgtatttag gaaaatttag tggattttga
    40441 aattctacat cagaattaaa ctccataaaa gttcctttat caatcaataa atttattctt
    40501 tttcttgctc ttaagccata atgtcttgaa cattttggac atacaaatga attttcttct
    40561 atgtcctttc taaatacatg actatcacaa cctttgcaat aggtccaaaa tttatcatct
    40621 acactattct ttttaaagtt gtcatctaaa gaaatcgtaa catacttaga ttcttttgat
    40681 gataaaaatt tttttatcat aatctattta ctccttataa tgtagcaaat tctttcttca
    40741 taaagacatc ttctataaat ttggtatgat gagtatcttc tctaaactcc tcacttgtta
    40801 caagtgcctt ttgaaattct acatttgtat taataccttc tacaataatt tcgtctagag
    40861 ctctatatat tctacttata cattcttctc tagtttcagc ccaacatatt aacttaccta
    40921 tcatagaatc atataaaggt ggtatagtat atcctgcata tacaaaagta tcaaatctaa
    40981 caccaaaacc tccaggtaaa tttaatgatt ctatcttacc tggtgatgga gcaaaattct
    41041 ttgatgaatc ttctgcattt attctacatt caatagcatg acctctaaaa gtaatatctt
    41101 cttgtttaaa aggaatttta tccccattag ctatagatat ttgaagtttt ataaggtcaa
    41161 gatttgttac catctctgta actggatgtt caacttgtat tcttgtattc atctctataa
    41221 aataaaagtt tttatctttg tcaactataa actcaatagt acctgcaccg atataaccta
    41281 ctccttttac tatatctact gcaattttat gaagtttttg tctttcttca tctgaaaggt
    41341 atggacttaa agattcttct attacttttt gatttcgcct ttgcatagaa caatctctat
    41401 cacctaaatg tattgcattt gaatgtttat ctccaaatac ttgtacttct atatgtctag
    41461 gattttctat aaacttttcc atatataaat cactattaga aaatgctgct agagcttctg
    41521 aacttgccat atcaaaattt gaaaaaagtt cttcttcttt tctaacgatt ctcatacctt
    41581 ttccaccgcc tccacttgag gcttttatca taacaggata tccaatttct cttgcaactt
    41641 ctaaagcttc ttccttagtt ttcaaaacag attctgaacc aggaacaaca gggatattca
    41701 attccatcat agtttctctt gctctagatt tatctcccat caaaatcatt tgttctcttg
    41761 ttggacctat aaaaataata ttattatctt cacattgctt tgcaaattca ggattctctg
    41821 ataaaaatcc aaagcctgga tgaatagcat cacactttaa tttaagagca agatagatta
    41881 tattttctat attattataa ctcttactta tattatttgg tcctatacaa atagattcat
    41941 cagctatata tctatgaaaa caatctttat ctatttcaga atagatagca acagttttta
    42001 tacctagttc tttacatgtt ctgatgattc taactgctat atcgcctcta tttgcaacta
    42061 gtattttttt tatcaactta aatacctcct actacaatgg ttttatctta aataaaggtt
    42121 gattatattc tactagttct tcattactaa ctaatacttc aattatttca cctttaattt
    42181 cacttgttat ctcattcata agcttcattg cttctaatat acataaagtg tcgccttctt
    42241 caactacatc gccaacttta acaaagctat ctgcatctgg acttggagaa tcatagaatg
    42301 ttcccatcaa aggagctact actactgata gattctcatt agatgtgaca ggtgtatcgt
    42361 ttacaactgg cttttctaaa gaaagatcca ctactgattc ttgttgtaca cttaaaacag
    42421 gtgaacttgt agattgagct tttttagata cttctaatct taaatcgcta ctttcaagtt
    42481 ttacatattc taagttagtt gagtctatgg cctttaataa ttccttaatt tcattgatgt
    42541 tcattttgtt acccccttgt attattacct ggtgctaata ttatatcaca aaaaacaaat
    42601 ttataaaaga aaaagtgctg actagagaat caacacatca atatatcttt taattttaaa
    42661 ttaacaacca gtaacttata tttgttttaa ttttccatta ttaatataaa ttataagtat
    42721 aaaatgaata aaattcaata ttttttgcaa aatacaagag aatacaaatt aatacctcgg
    42781 gaatttttaa gacaaatacc aatattatgc aatttagact tcgctttctt tcaaattagt
    42841 atcattttct ttatttttac ataggatgta ctttaattta gctatcaaaa cattgctata
    42901 taggttaaaa gttattagaa ttgcaggaat tataaagtaa actctagcac ctataaaacc
    42961 aaaaactact gtaataagta ttaatctttt atttaaagta aaaaaaggta tattaaaaat
    43021 acttttgtcg gatgtttctg gagtataaga atatataact ccaaaaatca aagtaagcat
    43081 acaaagtgga agtagtgtat aacttgaaat tattcccaaa aatcctatac tttcaaaata
    43141 tttgctagca ctatttgtat aaacaaacat gtatccattt aaatatgcgc atatacatga
    43201 tataatgcca aaagttcccc ctaaggattg agtgtaccaa ttaaaaaatt tagattccat
    43261 atacctcacc tcctaaaact gtatatataa tgatatgcat ttaaatagta aaatatatct
    43321 ttatatgttt ctgaataaaa ttttgttaat ttcattaatt tatgtttaag aaatttgaag
    43381 tatagtgtag tatatattaa tgtagatgaa gttttatagt acttatttca tagtgcttaa
    43441 tcaagttatc ttattatgga ttcaaaagtt gtttgtaggg ttttatattt tgttgagctg
    43501 aaaaatttat tataaatgaa gggatgtttt cgctttggta gatacattag aatttggtct
    43561 taaaatttta ttttttatat tatcaataat ttggatgggt aaaataatga ttttaagaac
    43621 tgataagcaa atagtaatca atcctttgct tattggtata tctgcaatcc ttgtgatgtt
    43681 gcatacctca cagagtaaca ttgaattttt tggattagat gtccaatata taagaatagt
    43741 tttgtatata atatattcac tgataatttt aataggaata tgggctacta acagaagaaa
    43801 tggaattttt tagataatct atttctataa aatttaaagg ttgctagtaa caaatcacta
    43861 atattagcgt tctgttacta acaaccttta ttttgattat acacttaaat tgaaaataga
    43921 atgattgaat aagttgacag attattttac attgacagta attagaactt cttgagatgt
    43981 atttaaatca tttttgtcgc ttagtataac ttttaccact gctttacctg ttttattacc
    44041 tttaattatc atatcttcac atgaaagtac actcttatca cttacagata caataaaatt
    44101 taaatctttt ggaaggttat cagtaacttc ccagtattta taataactat caattttctt
    44161 cgtaattatt tttctcaaat caatactttc accttttgct atatcaacat tatgatattt
    44221 agagtccagt ttatactgat tatcttttgt atcaataaaa tttaaatcgc aatttacatc
    44281 cacaccatta tgagaaagag aatttactac atcgtcatca atcaaataat tattggcaag
    44341 atttattgaa tccatatctt ttagacttaa agcttcctta ggaacctctt ttatattatt
    44401 attccaaaaa ataaaattgt ctacattttc aagatatgat atacctttta aatttgaaat
    44461 actctcacca gttgcataaa attctccatc taaagccttt atattgctta tagtcatctc
    44521 atctggattt atatatcttt ttacaactgc tcttaaattt gcatcaggaa aaatatcttt
    44581 aagttgattt tcgctcaaaa ccttatcatt tgaatttatt ggacttaaag ctgctgcaga
    44641 aataataaat ggtattaaat tagctaaatt catagtaccc tcccacataa attttttatt
    44701 ataataattt aatttctatg agagagttct gtttccttgt tatatatacc tactttcgta
    44761 cactttatta atataaatta aaataaattt ctactattaa acttataaac atattactag
    44821 ttacgttttt tagcttcaca tctaaatatt cgcatacctt gtgacataaa tttgtcttca
    44881 tactctgttg taacattatt ttcatactca ctatttccta aatcaagaga aatattcttt
    44941 aatagccaat tatttgcagc aatttcattt aaactaaact cgaataattt ttcattatct
    45001 gttttaaagt gaatttcgcc atcatcttcc aaaaccctat tgtacatttc taaaaatcca
    45061 gaatgagtga gtcttctttt actccatcta tttttaggcc atgggtcaca gaaatttata
    45121 taaattctcg ataattcttt agcttcaaag taatccaaaa tattgcttac atctccccat
    45181 aaaaatagta tattattcaa attagaagca tctgcttttt caacagcttt taaaagaact
    45241 tcttccttaa gttccatggc aatataattt atgtcagggt tctgcttagc taaagttgtt
    45301 ataaacttac cttttccagt cccaaattca acatgtattg gattatcatt tctaaacttt
    45361 aaattccact tgccttttaa tttgtctata tcgtctctta aaacatattt tgtataactt
    45421 aaaagctttt catctgcacc tttttttcgt cttcttctca caataattct ccttacatat
    45481 aaaaaattag taaaatacat ttttattata cattatctca ccaaaaaata aaaagaactc
    45541 tcttaaaata gaaatttatt tctataaaga gagttctaaa cttactaaaa acttaatctt
    45601 agtggcaccc tgaacaacca gagcatcctc ctccaccttg atctttcata ttttcaggaa
    45661 taactctaaa tccaaatcct gtatcttcta ttattatatc tccatactga ctatattcat
    45721 cagagctcat tacaaattgt aattctccaa cttcataagt taagtcatct tcttttttct
    45781 catctaaagc taatccaaaa gaaggacctc cacaaccaac acctgcaaag tatactctta
    45841 tattattagg tttatcttga ttgtctttta agatactttt taaagtatca atagctgttt
    45901 ctggtaaagt aattttcatc aaatcattcc ctttcaaata ttttattaaa acaatatcta
    45961 ttatataccc atacaaggta cataatcaat tagtttttgc atattttact aaaacttcta
    46021 cctaatatta cctaaggctt taaaatattt tata
//
LOCUS       DAMJZE010000013        45437 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJZE010000013 DAMJZE010000000
VERSION     DAMJZE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.013"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            57..896
                     /locus_tag="PC541_000633"
     CDS             57..896
                     /locus_tag="PC541_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2838112.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
     gene            1011..1250
                     /locus_tag="PC541_000634"
     CDS             1011..1250
                     /locus_tag="PC541_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838113.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1421..1597)
                     /locus_tag="PC541_000635"
     CDS             complement(1421..1597)
                     /locus_tag="PC541_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838114.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1651..1747)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2102..2776)
                     /locus_tag="PC541_000636"
     CDS             complement(2102..2776)
                     /locus_tag="PC541_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2838115.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(2996..3550)
                     /locus_tag="PC541_000637"
     CDS             complement(2996..3550)
                     /locus_tag="PC541_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2838116.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            complement(3628..3993)
                     /locus_tag="PC541_000638"
     CDS             complement(3628..3993)
                     /locus_tag="PC541_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838117.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4458..5243
                     /locus_tag="PC541_000639"
     CDS             4458..5243
                     /locus_tag="PC541_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2838118.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5340..6164)
                     /gene="pdaA"
                     /locus_tag="PC541_000640"
     CDS             complement(5340..6164)
                     /gene="pdaA"
                     /locus_tag="PC541_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2838119.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6385..9504
                     /locus_tag="PC541_000641"
     CDS             6385..9504
                     /locus_tag="PC541_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2838120.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9522..10121
                     /locus_tag="PC541_000642"
     CDS             9522..10121
                     /locus_tag="PC541_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2838121.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10348..12477)
                     /locus_tag="PC541_000643"
     CDS             complement(10348..12477)
                     /locus_tag="PC541_000643"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2838122.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12743..12991
                     /locus_tag="PC541_000644"
     CDS             12743..12991
                     /locus_tag="PC541_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838123.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13157..14548
                     /locus_tag="PC541_000645"
     CDS             13157..14548
                     /locus_tag="PC541_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2838124.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14663..16133
                     /locus_tag="PC541_000646"
                     /pseudo
     CDS             14663..16133
                     /locus_tag="PC541_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
     gene            16198..16791
                     /locus_tag="PC541_000647"
     CDS             16198..16791
                     /locus_tag="PC541_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2838125.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            16973..18319
                     /locus_tag="PC541_000648"
     CDS             16973..18319
                     /locus_tag="PC541_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2838126.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18501..18872
                     /locus_tag="PC541_000649"
     CDS             18501..18872
                     /locus_tag="PC541_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2838127.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18865..19554
                     /locus_tag="PC541_000650"
     CDS             18865..19554
                     /locus_tag="PC541_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2838128.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20074..20940
                     /locus_tag="PC541_000651"
     CDS             20074..20940
                     /locus_tag="PC541_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2838129.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21182..21991
                     /locus_tag="PC541_000652"
     CDS             21182..21991
                     /locus_tag="PC541_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838130.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22135..23199
                     /locus_tag="PC541_000653"
     CDS             22135..23199
                     /locus_tag="PC541_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2838131.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23227..23946
                     /locus_tag="PC541_000654"
     CDS             23227..23946
                     /locus_tag="PC541_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2838132.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23970..24554
                     /locus_tag="PC541_000655"
     CDS             23970..24554
                     /locus_tag="PC541_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2838133.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24556..25905
                     /gene="pabB"
                     /locus_tag="PC541_000656"
     CDS             24556..25905
                     /gene="pabB"
                     /locus_tag="PC541_000656"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2838134.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25895..26638
                     /locus_tag="PC541_000657"
     CDS             25895..26638
                     /locus_tag="PC541_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2838135.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26672..27373
                     /locus_tag="PC541_000658"
     CDS             26672..27373
                     /locus_tag="PC541_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838136.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27399..27971
                     /gene="folE"
                     /locus_tag="PC541_000659"
     CDS             27399..27971
                     /gene="folE"
                     /locus_tag="PC541_000659"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2838137.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28035..28826
                     /gene="folP"
                     /locus_tag="PC541_000660"
     CDS             28035..28826
                     /gene="folP"
                     /locus_tag="PC541_000660"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2838138.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28837..29202
                     /gene="folB"
                     /locus_tag="PC541_000661"
     CDS             28837..29202
                     /gene="folB"
                     /locus_tag="PC541_000661"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2838139.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29195..29701
                     /gene="folK"
                     /locus_tag="PC541_000662"
     CDS             29195..29701
                     /gene="folK"
                     /locus_tag="PC541_000662"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2838140.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29682..30467
                     /gene="aroF"
                     /locus_tag="PC541_000663"
     CDS             29682..30467
                     /gene="aroF"
                     /locus_tag="PC541_000663"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2838141.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30722..32512
                     /locus_tag="PC541_000664"
     CDS             30722..32512
                     /locus_tag="PC541_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2838142.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32530..33696
                     /gene="rpoD"
                     /locus_tag="PC541_000665"
     CDS             32530..33696
                     /gene="rpoD"
                     /locus_tag="PC541_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2838143.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33861..34550
                     /locus_tag="PC541_000666"
     CDS             33861..34550
                     /locus_tag="PC541_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2838144.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34564..35661
                     /locus_tag="PC541_000667"
     CDS             34564..35661
                     /locus_tag="PC541_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2838145.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35724..36296
                     /locus_tag="PC541_000668"
     CDS             35724..36296
                     /locus_tag="PC541_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2838146.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36356..36823)
                     /locus_tag="PC541_000669"
     CDS             complement(36356..36823)
                     /locus_tag="PC541_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2838147.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37209..37685)
                     /locus_tag="PC541_000670"
     CDS             complement(37209..37685)
                     /locus_tag="PC541_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2838148.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37805..38896)
                     /locus_tag="PC541_000671"
     CDS             complement(37805..38896)
                     /locus_tag="PC541_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2838149.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38889..39278)
                     /locus_tag="PC541_000672"
     CDS             complement(38889..39278)
                     /locus_tag="PC541_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2838150.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39470..39916
                     /locus_tag="PC541_000673"
     CDS             39470..39916
                     /locus_tag="PC541_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838151.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40180..40905
                     /locus_tag="PC541_000674"
     CDS             40180..40905
                     /locus_tag="PC541_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838152.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40892..42136
                     /locus_tag="PC541_000675"
     CDS             40892..42136
                     /locus_tag="PC541_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838153.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            42216..42893
                     /locus_tag="PC541_000676"
     CDS             42216..42893
                     /locus_tag="PC541_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838154.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42894..>45437
                     /locus_tag="PC541_000677"
     CDS             42894..>45437
                     /locus_tag="PC541_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838155.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNI"
BASE COUNT        18447 a         4635 c         7644 g        14711 t
ORIGIN      
        1 gagtaataat gttgatttaa aataaaaata agatttcgga ggagtttatt atataaatga
       61 aagattacta taaaatagga gagatatcta aaatatatgg aataggtaga gattcattaa
      121 tgtattatga agaaataggc attctaagac cagttagaga tataaatgga tatagaatgt
      181 ataatatatc agatatatgg aaattaaatt taataaaaga atttagaagc ttgaactttc
      241 caatgaagaa aataaaagaa tacctagatg acagaagtat agagtctacg aaaaatattt
      301 taaatgaaga actcgattta attgataaaa aaatagctga atttataagc cataaggaaa
      361 atattataaa aagattgagt tcaattgaaa gtgtaattca aaatacaaaa atagatgaga
      421 tagaggttgt atatatagaa aaaagaaaag ctttagaatt aaatgctgat ataaagaggg
      481 atgaagactt tgatttttta attcaaaaac ttcagaaaga atatgaagat agatttaata
      541 tacttggaaa caacaatatt ggttctgcct tttctactga agcaattaat aaaggcatat
      601 ttaatgagtt taaatctgta ttttgttttc tggaaagtaa tgagaaagta tataatatag
      661 tttttgatga aggatattat gtaactttaa attatactgg gagtaattct aataataaga
      721 tgtatatgga gaaggtgttt aaatttatag aagaaaataa ttataagata attgctgacc
      781 ctatagaaat ctataagata gatatacatg agactggaat tgtagaggag tttgtaacag
      841 aaattcaagt tcctataact aaatatctat atttcaatgg attagatggt aaatagaatg
      901 aaaaaatgtt gatttggcac cgatttgcca ctgtgttata aaatcggtag caaattatat
      961 tttaaattat aagatagcat gaaaaaataa ttgtttaggg ggttataaat gtgaaatgtt
     1021 ctaattgtgg aagtgtaaat attgaaaagg gaattttaac aactggtggc atatatgctg
     1081 aaactattgg attaaaatat agaggagatg gaaaattaga taaagttata aataaaactt
     1141 ttccagtaca atctactata tattcagatt tatgtttaga ttgtggcgaa atagttagaa
     1201 cctatataaa ggataatact gataggaatt ggtgtaaaga taagaaatag aataattttt
     1261 gaagttgaat tttattaaag atacaagtga aaaagtataa atgggaagaa attgttatat
     1321 ggaagaaaac tttgcatata tggaagatta gttttttaat ataataacat ttttaaatgt
     1381 agataaaact aaaaagggtt ctctagaaaa caatttctat ttaattatac ttacttacca
     1441 ataccatcac ttgatgtaaa caagattcta ctacattttc tccgtactct agtataaaat
     1501 catctagctg tgtatctata aaatcacaat aatcatataa actactaata taagcagtta
     1561 acatgtgtgt acatttatcc atttcatctc taaacatata tattactatc cccctaaaaa
     1621 actttcataa aacaaaaaga acaatatctt tttggcaact ggctgacata actcatagtt
     1681 tattttataa gcgttagtcc ctatagcttt gcgtcactaa atttctctag ttttgccgat
     1741 ttagttttat tctacaacta aaataataca ctaggataag ttattgttca acatacttgt
     1801 cagaaaattg aaatatttaa caatatctta acaatatgaa atctattttt cactcaagaa
     1861 acaaactata tgtatagaat gtttaataaa aattatattt agttttgtat tgaagttttt
     1921 gtattaaaaa gtttagttca caagggttac ttggctagat aatagataaa caaaaatatc
     1981 ttgaaaaaat atatatgaat ggcaaaaaac aagggatgaa ttttatatag actcatccct
     2041 taattattta tatattgacc ttgattaaat atattattat ttttaagctt caatttttat
     2101 attatatttc taaagtttct ataagaggat tatcgctaaa taattttaat aaatttgttt
     2161 ttttatatcc tttaggtaac ttagtatata taactacttc taaccaatcc ccatcaaggc
     2221 gtttaatatt catgtttata atttcaatat tagatttttt aaatttacct tcaatatatt
     2281 ctatggaatc agtgctgtta gaaattttaa gagaaacttg ctctgctgta gggagtttca
     2341 tccatttcct atctctatga agtgttatct gtacaataac aataatgata cttgaaacaa
     2401 taccaactaa gtataatcca gagccaatgg caagacctgt accggctgtt gcccatatac
     2461 cagcagctgt agtaagacca cttactgatt gatttctaat aaatattaat ccagcaccta
     2521 aaaaacctat accactgaca acttgtgctg caacgcgggc tgggtctaga ccaatacctg
     2581 gagtacccac tatatcatta aatccatact tggaaacaat gattataagt gctgaagcta
     2641 atgccactat aaaatgagtg cgtattccag cttcttttag tctattcttt ctctcatatc
     2701 caattaaagc cccacagaaa ccagcagtaa tgattcttgc tatatattca aattgaatca
     2761 atatatgatg gttcatttat ctagcacccc cttctatata atattatata tactataata
     2821 ctacattttt tatgtgaata tagtaaagtt taaaatttat ataatagtta tatatgaata
     2881 taaaatgtat aaacgtatat tatgtatata aaaatgatag aaagcatcta attttaaatg
     2941 attaaagagc aagcttaaat aaatcaaact tgctctttta acatagtgta tgtttttatt
     3001 tgtaatagtt agataaaaat ttatctgtgt tttttacatc tgcaaaactg ttctgtagtg
     3061 atgccatgaa tacttgatgt acagtatttg cattaatttc tatattattc caagataaat
     3121 tcttcgttgt acatgcatcg cttattagag ttatgtcgta tcgaagtttc tttgctgttc
     3181 ttacacttgt atcaatacac atatggctca tcattccaca gattactaga ttagtaatat
     3241 tttttttctc aaggatactt tgcaaatctg tttcaaagaa actatctggt gtgtgtttaa
     3301 ttataatttc ttcattatct agtggaaaaa caattttatt tatttctgaa ccatgagtat
     3361 taggaagaaa aaaagaagct gttttatctg ttgatatatg ctgtatatga ataactggta
     3421 aattgttttt tctaaaaaac agtagtattt ttttagcatt ttcagcagtt tcttctgatt
     3481 gaaataattc acattttcca tttttaaaat aatcattttg aatatcaatc aatattaaag
     3541 ctatagacat gattatacct cctatttatt tttattataa tactattgta aaaaatgtaa
     3601 attacttact ttttggtatg tataggatta cttattgtag caaattcctt tattatctag
     3661 aatttcttgt tgaccatgtt caagcatata ttcgattcct atttcttgca ttatttccac
     3721 tgcttttaac atttttttac cacgattagt aagaaaatat tcaacatgta atggatatcc
     3781 agcagaactt attttatcaa taatcccaaa atcacgtaac tcttttaatt gttgaatcaa
     3841 cattttttga tttattccat aaatattatg ttgcaattca gaaaatgttt tattagattg
     3901 tcttaattca aatataagga tagttttcca ttttcctttg attatgtcat gtacaatttc
     3961 aagaggacat gtatattcag ctcttatttt catagcatat cacccccagt aaaatgataa
     4021 taaattatat cacttgtctt gtgatatgac aaatatatat gaactgtata taaattttat
     4081 gttttattaa gttctaaatt tcttctattg tagttcatat ttagtacata aataattagt
     4141 aagatataag taaataaata tataacatga ctatagaaat ttaactttaa aataacttat
     4201 gataatttag aggaggaata tatatgaata tatcaagaaa agctatgaaa ataatagaat
     4261 tagcacaaaa aattgcaaat aagagaggta tttcagtaga agaagcttgg agtgaagcag
     4321 taacagaata taaaaataaa tacgaacata ttgcatagat atttaaaaat ttagaatatt
     4381 tggtagaata taatgatttt aaatgatact ataaattata aggtatatca ttattaacat
     4441 agaaggagga tatctgcttg attgtaaaaa tagataatac tttagaaaaa gaattttggc
     4501 aatatgtatc acatgaagaa agtttaaatt tattcataat tggatacgtt gagaattatg
     4561 gatttagctc tcaatatcaa gatatatggt ctcaggtaga aggtggaaat ataacctcta
     4621 taatattaaa aaataagtca acacttataa tttatagttt taaaaataat tttaatatag
     4681 gagaaatgaa gaatcatata aaagatttag atgtagaaag cataagtgga aaaaaatgtg
     4741 taatagatag actaataagt aaatacaaag atttttatga aaaactagat aataaatttt
     4801 gtgtattaaa agaaataaaa gaaatagatt tttccaacat gaaggagtat aaaatagaaa
     4861 atgctcagga aaaagatatt gatgaaatag gaaaactttt aaataggtca gactataaag
     4921 taagtaaaaa ttacatagaa gaaagaaaag ttcatttaaa agagggaaat gtaagagcat
     4981 attttattag aaatgatgat actatgatta gcactgtgtc aactggtatg gaaactagtt
     5041 ttttggcaat ggtggtctct gtaagtacag ataaaagata taggggaaaa ggacttgcaa
     5101 gttatatggt ttacaatttg agtaaagaat tgttgttaga aggaaaagtt ccatgtcttt
     5161 tttataataa tgatgtagca ggtaaaatat atcataatat aggatacaaa gagataaatg
     5221 aatggacaat tctttttaaa tagctataaa aaataaaaaa ctctatcgag aattaaagta
     5281 tttataaata actatatttt tactataatt catgatagag tttttttaaa tttatgatac
     5341 tattttttat tatttaaata atcaagtgtt ttaagtttat aaccttcttt ttcccaatgt
     5401 gtaagaacct catctaatat ctcagtattt gttgatgaat ttggatgcag taaaactatt
     5461 gcaccaggat gagttcttga ataaattttg tttttggcaa attcatgagt aggttgtttc
     5521 ttttcatacc aatctacata agcaaaactc caaaatattg atttatatcc taaatcttgt
     5581 gtatatttta aagattgttc gctaaattta cccattggag gtctaaaata cttaggcatt
     5641 tcttttccag taacatcttt gtatgctttt tctacacttg taatttcttc tttgaatttt
     5701 tcaaaatctg taatacctgc cattgatgga tggctttttg aatgattaca aactaaatgt
     5761 ccttcttttt ccatacgctt tattaactct ggattgcttt ttatataact ttcaactacg
     5821 aaaaattgag ctttagcatt gtgtttttta agcgtatcta ataattttgg agtgtttcca
     5881 ctttcatatc cagcatcaaa tgatagatat ataacttttt cattgggatt tcctacataa
     5941 taagctccgt attttttaaa aaaatcagct tcttttatag gtgatggctg tttaccatct
     6001 tccctagggt taaaatacca atcatattca tgtgtgtcga gtgttggact agaaatttga
     6061 gtttttgttt tatctaatga tttaaaattt atactagcaa ttccaaaaag tgttagagca
     6121 aatgctccta tcataatgta tttttttagg ctatcttttt tcacaatttc acctcttatt
     6181 taaaatttaa agtatatttc ttataatata gtttgaatat ttatagttaa aaatattcac
     6241 atatacaaat aataaatgaa agaagtttac tgtaagatat aaagttgata ttaattatct
     6301 taaataaagt gtatataaat gataaaatat aaagagaaat gattgtaaat aagttaggct
     6361 ggtttaactt gaagggatat aaaaatggat aatataataa gtgtattgaa tgatgttgtt
     6421 tttttagata tagaagttag tggattagat tgtttaaatt cagaaatttt agaagttgga
     6481 gctgtcaagg ttaaagactg gaaaatatat acctatgaat cattaataaa aaacaaattt
     6541 gaggtaccta tagaagtatt ctcagtatgt aaaaatttag ataaaaatga tttagaaata
     6601 gctaataaaa tagaactggt ggaagataga cttgtaaatt ttgtagaaga tagtttcatt
     6661 atatgtcatg atttaagtct gaaaaaaaag ttttttgagt atcatatgcc aaagttaaaa
     6721 aataaattta ttgatttaat tgaattagca gttattttag aaccatatca taaagattat
     6781 agcttagaat acttaaaaaa tactttaaca aattgtaatt ctaaagtgga aaatagagct
     6841 ttatcagatg ctattgatat aataaatatt gtaaattgcc ttttagtaaa atttaataac
     6901 tatgaaaaaa ctactttgga gccattgagt tttaaaataa attcatatct taaaaaattt
     6961 aatcttccaa catgggaatg gagcaaattt ttagaagagg caaattatga tttatcaaat
     7021 aatataaata taaaaaaaga atataatata tttgactcaa aagaagaaaa gaagaaggaa
     7081 agagaaacct taaaaatttt aaatgaagaa gaaaaaaatt atgaagaatt attaaaatac
     7141 aaaactattt gggagaataa agaaggattt acatatgaat atagacctgg tcaatatgag
     7201 cttactaaaa caataagaga gttgtttaga aatagcgaag atgaagaaaa aattgcatgt
     7261 atagaggcac caactggtat aggtaaaagt gtaggatatt tattgcctgc aatattagag
     7321 gctagaatta ataaaaaaag attattaatt tctactgaca caaaggaatt gcagatacaa
     7381 ttaataaata aagatattcc aaatgtgtta gattctttgg gtctaaatgg aaaagtaagt
     7441 tacggatata taaagggaaa aaataactat atttgtatag atagactgga agcatatata
     7501 gatgattatg agtctcaaaa ccctactaaa ggagaaattt tatctttaat atttttgaag
     7561 agattagttg aaggtggcaa gtatggagat atagaagaaa taaattattt agtgtttgac
     7621 aacttcaagg aaataagtac tcacttaaga aatgtaagtt gtgacccaaa tatgtgcaga
     7681 cctaaaaaat gcaaaaaaga ttgtttatat aaaaacagaa tagaagaatt aaaagaagaa
     7741 catataacag tagtaaatca ttcactatta gctaaatggc cgtataagga tgaaaaacct
     7801 ttagaaaata taatagtaga tgaagcacat aatttgactg aaaaaggata tgatttcttt
     7861 tcaagtatta taaattcaaa gtctttaagg tacttattac aagaaattta tccttacgag
     7921 tttattcaaa atagtagctt tatatataaa aaatatagta gaaatatgag aaaaataaag
     7981 gcttttgata aattttataa tgtattaaaa attggaagag aagataaaca aaaaattgct
     8041 agaagtataa atttcattat tgaagagata gattcaatat taaagtttgg taattgtaat
     8101 gaatataata atgtaagtaa ttataattta agatgggaac tgaatttgca aatagatgaa
     8161 attgtgggaa aactaaaaaa agatggtata gatactgaaa taagttatag agcctatagt
     8221 gaaaagataa agttgagttg tgagaaaata ataaaaaatt tagtttcaat aataataata
     8281 atttatagga atattgatga tgatagtata gataaagaag cagatatata taaatttgga
     8341 aaggcaaaga caagagattt agaggatata aagattatat ttgaaatctt tttagagtat
     8401 gatgaaaagg atgattatgc aaggatagtt gaaattgata agaattataa tgactttgaa
     8461 tttcgagtag taccattaaa aatagcagat ttatttgaag aaaatatttt atctcaatta
     8521 gaaaaaggaa tatttttatc agcaactttg agtttatctg aaagtatgtc ttattttaaa
     8581 aatacattag gaatagacag agttaagaat gtggaaaaga ttatagaacc aatttttgat
     8641 tataaaaata gagtctctgt agttggattt tctgatattt gtgagtacag aaattctgaa
     8701 tttccaaatg agatgagtaa aattatatct aatatatcaa agattacaga aggtcataca
     8761 ttagctcttt ttaatagtaa agatagacaa gaaaaaactt atgaaatatt gaaaaaatac
     8821 ttacatagtt ttaacttaga aatttatgct gataaaaagg gcattagaca ccttaacgat
     8881 ttaaatagaa aatgtgtagt acttggctca aaaggatgtt ttgaaggcgt agacattcca
     8941 ggggatggtt tggtttgtgt aactttagac aagcttccga atctaaatcc taaagaccct
     9001 ttgtatttta ctataatgaa gaagtatggt atagactact atactataaa ttatcctcaa
     9061 atgaccataa aggtaaagca agctatgggg agaattttaa gaagcaaata tgactatgga
     9121 tgttttgtga tttttgatgt aggaactaat ataagtgtgt taaagagatt agagaaagat
     9181 ttacacgatt gtaaaatttc aaaggttaat agcaatgagt tttatactta tataaggcga
     9241 catttaaata aatctagaag tttaatacta aaatctgtta tatttgatac tataaaagcg
     9301 ttgaatgttg atgctaaaat ggataacaat gatgttgata aggatattat aaaaaaagat
     9361 ataaatgaaa atataagaca aagagctgta aaaggagaag tgtaccatat tgatattata
     9421 aaaaaagata tgaaagtaaa atattttgat agaaactatc ttataaattt agatatattt
     9481 atgagagaag aagataaaaa ttaagagtgg gagtgatata tttggcaaaa caaaagtttt
     9541 atgctgtgaa aaaaggtaaa aatataggtg tatataatac atgggatgag tgcaaaaaac
     9601 aagtaaatgg attctcagga gctgaatata agagtttttc tacttttcaa gaagctaaag
     9661 aatatattga tgggtcagaa aaattgagtt ttcaagaaga taaggagttt atagaagcat
     9721 atgtagatgg aagttatgaa cattcagtaa aaatgtatgg ttcgggtgtt gtgattttaa
     9781 aaaataatga agttataaaa acttatagtg aaaaaggaaa agagaaaact ttagtaagta
     9841 tgagaaatgt agctggagag atagaggctt ctaaaattgc aatgcagtac tgtattgaca
     9901 ataatgttca aaacttgatt ttatactttg actatgaagg gattgaaaag tggtgtactg
     9961 gagtttggaa aacaaataaa gaaggaacta tagcatataa aaatttctat gacagtatta
    10021 agaataagct taatgtaaaa tttacaaagg ttaaagctca ttctggaaat aagtataatg
    10081 aggaagctga taaattagct aaaaaagcta ttggtgtata ataaaaaaat atttatacat
    10141 aattacatgg tataagagta tatctaaatt cctactttat aattaaacaa cttgtaatta
    10201 aataaatttt gattaaaaaa gcagattgta ttatgaaaaa ataagtatct aatttataaa
    10261 caattaataa tagtattaat tagatacaac ttatttaata taataaaatc tgctttttat
    10321 ataactataa aattttacta cattctatta gaattgccca taaatacctt caagttcttc
    10381 ttttaatact tcagggtcat ttataccaat agacatggtt ctagccagta tatttgcttg
    10441 gtcataagtt gatgtccatg agaatacacc acctagacaa tttctaagaa catattgagt
    10501 ttttaagaat attgataaag catcatcata tgacattgca aaatctccat ccttaactaa
    10561 atacggaact tgagcagtat tatcaaatct atattcatat ccatttttat ttatataatc
    10621 ccttctaagc tcatcataag ttcttgttat agtagcacct aatcttccat aaaatggtat
    10681 accaagaagg attttttcag aaggcattcc agcattctca agatttctta ccattgcatc
    10741 aacactatat cctggcaaag ataagtctga atcaaaaaga tttgcttgat gttttctacc
    10801 atttgggcct gtttcacctg ctgtaaaatc ataactcata agattaaaat aatctattat
    10861 aggagctatt ttatctattt cagcacttga atttatatat cctctatctc ctgttccagc
    10921 tacactaagc catttatcat ctcctataac atctcttatg gcagttagta agagtgtaaa
    10981 gttttctcta tcttgaggtc ttgatgttat tccagatgca ctacttccag gatattccca
    11041 gtctatatct attccatcta aagcatattc atttatcatc tgattgacct gtcttgcaaa
    11101 attatatcta gatgtaggtg ttaatgctgc atcagagaat ccttcagctc cccaacctcc
    11161 aatagctaca attactttta agctaggttt ttcaccttta agagcaagta attgatttaa
    11221 aaatctagga taaggaataa acaaatttcc agactggtct atctcagcaa aagcaaatat
    11281 aactgcatct aatagttgag catctacatt agttggattt ccaagaacat attctgctac
    11341 aattggattt atatctggac agtttgcatt ttcaactatt ggattgtatg ggtctgttat
    11401 ttgtggtcta actaattttt tagaatcttt taaagttttc atagctattt ttttagtatt
    11461 tttttcgcta gtataatcgt agtttttata attatgttta tagaaatctt cataactact
    11521 atcttctttg ttatagctag aatttatttt gtcacaagat tcatctttat catagttaaa
    11581 attcatttca tcacaagact tatctttaaa cttatcttgt ttggagttgt gaactttgtt
    11641 agtattttta gtatgttttt tctcatgatt gcaatcatct ttaatatcag tcttgtgttc
    11701 ttgtttacag tcaagagctt tgttcaggta gtcatgatgt tcatgttcac agttacattc
    11761 attttcaatg tttttagtat gttctttttt ccagctacaa gtattatcaa ttttcttgct
    11821 cacttcttca tcattataat tatctggtac aaaacataag taccagtcca tacatgaata
    11881 tttcttatta caactgttaa ctctattttt taattcctta tagtttttag gataaggtaa
    11941 aatcactggc attccaggaa accagtttgc aggagtaact atattatctc tatcactagt
    12001 ctgaagtgca tctactattc taagtatttc tggaatattt cttcctgtag ttagtggata
    12061 ataaagaatc gttcttagaa tttgattatt atctataata aatacagagc gaacagttga
    12121 tgtatcactc attggttttg atatcatgcc gtataacttg gcaattctca tatctctatc
    12181 ttctataata ggaaatggaa tttctacacc tgtaagtaaa gaaatattat acatccaagc
    12241 taaatgtgaa ctgttactat caacacttag accaattagt tctgtatttc tttttttaaa
    12301 ttcgtcataa tatttagcaa aacataaaaa ttctgtagta caaactggtg taaaatcacc
    12361 aggatgtgaa aataaaacaa tccaattacc cttatagtca gagagtctaa taggaccatt
    12421 tgttgtattg gctttaaaat caggagcctt tgaccctaaa cttggcaaat ttggcatgta
    12481 aatcacccct caataaatta tcatttattt tattctatta attaatacat attagtgtta
    12541 ctattttatt aaaagttaag taatccttat gtcaaaaaaa gatatttatt aaaaagattt
    12601 aatattttcc taatataaat gacaatagta attgctattt ttttactaat ttggtatagt
    12661 aatggataag gggtagtgtc aattttagtg attatacgag gtgagtggat tgaaaaaaat
    12721 tctaagaatt ttagttatct ccatgatgat atgttttgca atgacttttg cattagtgtt
    12781 aataaattca gttatgggag tacatatagg attggattat tactttttga tgggaagtgt
    12841 gcttacagtt tgtttaacaa ttggaattgt tttatgcaat gctagaaaaa tatttgatag
    12901 caaagctata cacaagacaa gaacgaaaac tgtcagtaat aataaaaaac aaaagagtca
    12961 ttctagggat ataaaaagaa agatatcata atttatgtaa aggaaatatt tatgtataaa
    13021 ataatgattg ataggtaaaa taaaaacacg atttaacttg tgtttttatt ttttttcaag
    13081 aattatgata taataaccaa taagaaattt agtttaccat aaaatataaa gattattaaa
    13141 tggagaggga actataatga attttagaga tttatttgag aaggctgtag attttattga
    13201 tgaaaaaagg aaatctatag ttgcgatttc attatcaaca ttaggtgtaa ttgctttgat
    13261 aatagtattt ttcttaagta gtaacgagtt tagtgtagga aatgaagcta atgagttgtt
    13321 aaagattata gagaaaagac agtattctat agctgttgat tactacacaa gtatagaaaa
    13381 aaagttttct gattcaaaaa tggaaaggtt taataagtct gtttctaaaa aaataaataa
    13441 attgctatta aatagtggtg ataaatattt agatggagac ataagtcaag aaagttttat
    13501 tggattaata aataccgtga aagaattaaa caaaataagt atagatgtgg atgacttatt
    13561 agctcaagct gatagagtta gagaaatgta taaagaagaa aatactactt atgatattgc
    13621 cattaactac ataagtactg tttcaatatt aaatggtatg ggtagtaacc tagatgtata
    13681 caaacagaat atagaaacaa tcaaagagtc tagagatgtt tatgactcag cagtaaaaga
    13741 ccaaaaagca tataagtatt atgaagctat tgaaaagtat aataaagtat taaaagaaga
    13801 tgaaaagtat tacagtatgg ctcaaaatgg gaaagaacaa tgtattgaag aaatgtatga
    13861 ttactatata agtagagctg aagaggcaaa tacttctgga gattatgaaa gagcattaca
    13921 atatattgag tatttaaaag acgattattc agatgatgaa aaggtacaga gtttagaaaa
    13981 aaaatataaa aagaatttat cattgtatac tttaacatct gatgacatta taaatgtaat
    14041 agctaaaaaa agtggcaaag acaaggcaaa tctatctata aattcacttc cacaaatgat
    14101 aaaaaataat aaatactatt atgctgaagt atatgagtat gataaattaa taaatgaagt
    14161 tttggtaagt gcaaaaacta aagatttata ttcatataaa gatggtaaaa aagattacaa
    14221 agtagattat ggtgatggat actttagaat attagaagat ggaagttatc aatttggtat
    14281 tacaaaagat aaagctaagt ttgtgttaac taatactctt gatgaaaaag aaaataagta
    14341 taaaaagatt gagatattag atatagagaa agcagataga tatgtaaaaa gtaaaaagag
    14401 tttagaagaa ttgtttggaa aatataaaaa tatttattat tatgctgtag taaataaagg
    14461 cttatttaga ggcaaagaag tttatgctat taatatttac aatgaaaaaa tatatgccat
    14521 atcagaaaat ggactaaatg aatattaatt aataatatta aattaatata taattaaaaa
    14581 gtttttgggg ttttaaggcg cattatgtag taatataata atagggatat ataaataatt
    14641 ttattgtaag gagaagtatg ggatgagtaa tgtgaacaaa aagctagtta tagctattgg
    14701 agctgtagca ttagtagtaa taatttttat aggcataatg tgcatgcaat ttaaagggga
    14761 gaaaatagca aaaaacacat atgttaatgg agtaaatatt ggaaaattaa ctaaatcaca
    14821 agcaaaacag gaattagcta aaaaatacaa attagagaat gttgatttta attacaatga
    14881 taaaagctgg aaagtaaaga gcaaagattt aaatttatct actgacctgg ataaaacagt
    14941 agaaaacgca tataatttaa atagaaagag tgctttcttt ggcaatctat caaaaactat
    15001 ttcagcaaat tttggcaaaa aaagcaactt agtagttgcg ataaattatg ataaaaataa
    15061 attaaaagca gaaatggaaa agatagctaa agaaatagat gtagatgtaa aagatgctac
    15121 acttgatata agtggtgaaa aagtcaaggt tataccagat tctgatggtc ttaaaatgga
    15181 tatttctaag agtatggaga attttgataa tcaaactaaa aaaggtaact ataaaaatga
    15241 gttagtagta aaagctactc cagcaaaagt aaaaaaagag caacttgcta atatagatac
    15301 aaatttaggt acgtattcaa caacatttaa aacttctcaa ataaatagaa gtataaacat
    15361 aaaattagca acagataata ataagtaatg tattacttat gccaggagaa actttctcat
    15421 ttaataaaca tacagggaaa agaagtaagg aaaatggata taaaagtgca cctgtaataa
    15481 tggaaggtga gatggaagaa gattatggtg gaggagtttg tcaagtttca tctacgttat
    15541 acaattctgt gctatacgca ggtttagaaa ttgttaatgt aaaaaatcat actataccat
    15601 caagttatgt tccaaaagga agagatgcaa ctgttgcaga tagtggaata gatttcttat
    15661 ttaaaaataa tctaaagcat ccagtttaca taaaaaatta cgtttcagga aatcaaattg
    15721 tttgcaatat atatggaagt gcagaagata aacaaaatat aacaatatca acaaaattag
    15781 atggcgtttc acaaactaca atgaaaagag ttaatgaccc tacaatgcct aaaggtaaag
    15841 aaaaagtaga caaaagtgga agaaatgcat attctgtatc aacatataga acatttaatg
    15901 atgcaaatgg taaaaaaatt aaaactgaaa aaatagctaa ttcatattat cctaagaagg
    15961 aaggtattat attagttggg actatggaac caaaaccaga ggaaaaacca aatacagacg
    16021 aaaataagaa taatcaaaat acaaacaatc aaaacccaaa taatcaacaa aaaccagaaa
    16081 caccaccaac tgataataag cctaatgaga cacaaccaca accgcaagct taaagttctc
    16141 aagaatagga tttatgtaga gtaataaaaa atataaatct aaataggagg gattactttg
    16201 aatttaaata atataacgaa ttacgttcaa agtatttgcg agaatatttc taatgtcttg
    16261 gatgtagatg ttactttagt tacaaaagat ttaactagga tagcaggtac aggaatattc
    16321 agaggtaaaa taggagagac aatatcagag aagtcagtgt actctcttgt tttaaaagat
    16381 gggaaatcct atgttataaa taaagaaata gaagagaact gtaataaatg tgcaaataga
    16441 gaaaattgta aagagttagc tgatatatgt actcccgtta aaatgggaga gcatatactt
    16501 ggaatcttgg gtatagctgc gtttaatgaa gaacaaaaag aaaagatact ttcaaaagat
    16561 aaggagttaa ctgcttttgt aaggagtatg tctgatttaa tatcttttaa actagatgaa
    16621 ctttataaac aagaagttaa gactatagat gaccttgaga gggaagctat agaaaatgcc
    16681 ataaagaaat atggaagtaa tacagaaggt atgaaaaaag tagctgaggc attaaatata
    16741 ggtatagcta cactctatag aaaagtaaaa aaattcaaca ttaaaagtta atagtattga
    16801 aaaagaggta acctttttat gaaaagaaga ggattacctt ttttattatg agaatggtgg
    16861 aaaaatatgt atagttttga cttgacatat taaatatgtt atcttaaata tatattttag
    16921 agtggaggtg agtattattt tagttaaaga tataaaacaa gacatagaaa gcatgcctag
    16981 aaaaatttta gatatatctt tgatagaaga taataaaagt gatttgactt tttttaatat
    17041 agaagaagaa gacggtgatg aaacattaaa agcattaaaa cagagttatg tgactacaaa
    17101 ttataaagaa gaaactctat taaacttgca aggagcttta aattcaaagt cagatacaac
    17161 gtctaaggag ttaaaattag aatcatataa aagatataac aatgcattgg atttagcata
    17221 taaaaattat ataactagtg ccattaatat ggttaataaa gctctggaga taaatcctaa
    17281 agacattgat atactaaatc ttagaggtct tttaaaatta ttaaaatgtg attttgctaa
    17341 atcatttgag agtttttata ctgctttatg ttatgaaaat aatcatatat ctagaaaata
    17401 tgtaaatcta atttcatctg aagattttaa aactttttta ggaagataca atcattctat
    17461 aagatttata aatgaagaat taaattatga gtcaatacat attttagaga acataattga
    17521 agaagaacct gaactaatag agccatatgt aattttatct ttgttatatg ataaactagg
    17581 taatgtaaaa aagagagaag gatatttaga caaacttaaa gagctagata aagataatcc
    17641 tgtatttgaa aaaaatgaag aagaaaaaga agatacaagt aaaaaggaag aaataaaaaa
    17701 agtaaaaaga aagaaaagta ttttgccata tattatagtt ggctgtgttt taataggtat
    17761 ggggatatat ttgattcaaa gcaaaaagag aatagaaaac ttgaataacc aaataagtag
    17821 taaagaggaa aagttaagtg aaactgataa aaagttagga gaaacgagta aaaaattaga
    17881 aaaaacgaat aaggaattaa atgaggttaa aaataaagaa ccagaaagag agataactgt
    17941 agctgatgag gaagatttat attatcaagc tctaaattta aagaaaaaca aagaatatga
    18001 aaaagccata gataatttta aaagtgtagt ttcttctggt aaaactaaaa aatatatttc
    18061 tgagtcaata tatcaattag caataacaaa taaattatta ggaaataagg atgaagctat
    18121 aaaatattac aaaaaatata taaatactta tactaaaaat gaccagtatt atgatgattc
    18181 atattacgag ttgggtatgc tttattatga taatggcgat ttaaaaaatg cacaacagac
    18241 tttttatagt ttgagaagtg aagttccaga cagtatgtat aacaattcaa aaataaaaga
    18301 aattttaagt gaaaaatagg tttatatagt taagtttttt gaaacaacca agtatgtatt
    18361 ttattaaatt aaaataaaaa taaatttaaa aagagattgt gtcaaattat aactttctat
    18421 tttaaagaaa gatatttgct tgttgataaa actacaaata tatgatttga agcaatctct
    18481 ttaatttttg gaggtggaaa atgaaagttt tcaatcaatt ggtaataatt ttatctatct
    18541 gggtaatagg agagtatata agttccttta tacaaggaat tatacttatt cctgggagta
    18601 tagttggtat gctattatta tttctactac tacaatttaa agttttagac ttgagtagta
    18661 tagaaaatgt aagtggattt ttcctcgata atatggctat attttttatt ccagcaggtg
    18721 tatcacttat aaagtcatta gacctaataa gggaaaatgt attagtgctt ttattagtca
    18781 tatgtttaag tacactaata gtaatgtata ctactggaat tattgtggag aaaatgataa
    18841 aaaagaagga agaaggacaa aaaaatgtat aatgtattac agacaccagt atttggtata
    18901 atagtaacaa tagttttttt caatttagga agatacattc aacgaaaaac aagtaatcct
    18961 atatgtaacc ctttattaat tgcaatcgta ggaataatat tgtttttaag catatctaaa
    19021 atcccttatg agaattacaa gataggagct gatagtctaa atttcttttt aggtcctgtc
    19081 acagtagtac ttgcagttcc actttataaa caatttgaat tatttaaaaa gcatatgttt
    19141 gagattattg taggaatagg ttgtggtata gtgatttctt ttgtatctgt attgataatt
    19201 ggcaaaatag ctaattcaga tgtaagtata ataaattcac taattccaaa atctataaca
    19261 acaccaatgg gaatatcttt agcaaattct ttaaatggtg tagaatcaat aacagttgta
    19321 gccataattt ttacaggtat ttttggtggt atggttgctt ctacagtatt caaactgggg
    19381 aatataaatc atccagttgc taaaggaatt gctcttggaa catctgcaca tgctttagga
    19441 actacaaagg cttttgaact tggagaaata gaaggagcta tgagtggtgt ttctattgga
    19501 gtctctggta caattacagt aatactaatt ccaattataa tgaattttat ataaaaaata
    19561 aaattaagac gagtaacttg aatacttaaa acaaaataaa ataaaaaaaa tttaaaataa
    19621 aaactgaacg aaaaaaatgc aaataatgaa ataaaaaaat catgtactga aattataaaa
    19681 agaaaaattt catttaggaa ttaattataa agagacaatt actaaacttg aatttgctaa
    19741 aataaaataa attactgcga aaatagatat ttagaaaaaa atactataaa tttactatat
    19801 tgcaagttaa ataataagca gtatcatatt atgctagaaa aagtttcatt taaggcataa
    19861 atgagatgat ttgaacgttt ttaaagatta gaatataaaa gattgcacta gacatatatg
    19921 aaaaagataa tataatagat gatgtaatag atgacagttc aagaaattat aaaaaaaatt
    19981 tatttgaaat attatctatt acatcaggaa taatatatgt aagagtaagc ttttaaaatt
    20041 ctaaagattt agaaagattg aagggagaaa gaaatgagtg aagttaagaa taccctacaa
    20101 aaacatccag ttttaagata ttcaacaatg ctatttggga tgttgatgac atctatagga
    20161 ataaatgggt tcttaagacc agcacatcta cttagtggag gtgctacagg tatagcaaca
    20221 tctataaact atttgacaaa tataaatgta ggtttattaa catttttaat caatattcca
    20281 atatttatct tagggttcat ataccttgaa aaagagtttt gtatttcaag tttggtaaat
    20341 atgattgtat tttcattatt attaggagca actcaggaaa taagtaatat tatacctata
    20401 catgatatat tgcttcaaag tgtatatggt ggtatattat ctggtttagg agtcggagtt
    20461 gtatttagaa ctaggtcttc tcaaggtggt acagatataa tagctgcaat actaaaaatt
    20521 aagaagaata tagaaatgaa agacactgct ttagcaataa atggcttgat agtacttaca
    20581 ggaagttttt tatttgggtt ggatttagca ttatacactc ttataggtct atttctaaat
    20641 gcgtattcaa tgagttttat taaagatgca atgaattacc aaaaatctgt tatggtaatg
    20701 tcaaatgaag tcgatttaat agcagaagat ataatgaaga gtttagttag aggtgtaact
    20761 tttttagatg cagaaggtgc atacactcat caaaaaaaga agattatata tacaatagtt
    20821 tcatcaaatg agataccaaa gataaaagat atagctttga agtatgataa aaaggccttt
    20881 atatctgtaa atgatgttac agaagtaaaa ggaagagggt ttaaagcaaa agatttatag
    20941 aaaattttac aagctgggag tttataatgt aaatttgtat attatatctc tcagcttatt
    21001 taaatttagt atacgaaaat aaaggtttta ttaatgattt gtgtatcact ttaaaagata
    21061 tcattttaaa attaaaattg aaaaaattac ttaatatgta gaaaacatga ccactattag
    21121 gatataataa atttaataga tttagtaaaa tatctaggaa taacgttgga ggaatggcat
    21181 tatgaattta tctaaagaat atgttaattt gtatgataat tatttgaaat atataaatga
    21241 agtaaacaaa gagattagaa gtataaagaa catgaagaaa cgtgttttaa aaaggggcga
    21301 ttttgctcca gaaaatgaag tattagaatt ggaaggaaaa acaatacgcg atatagatga
    21361 tgtcgataca aaaaaaccaa aaaataaaat ctataaattt tcaaatgggg atgtatatgt
    21421 agggaaattc ttagagggaa agatggaggg gaaaggctca tatacttttt ttgtagatga
    21481 aggaacagtt atggagtaca taggagaatt taaagaagat aaaaaaaatg gaaaaggaaa
    21541 ctttatattt tcaaatggaa atgaatatat aggttatttt gaggaagata tgatgagcgg
    21601 tattggaaat atgttatata gtagtaaaga tgaatatatt ggaacttgga aaaacggaaa
    21661 aaaagatgga aaaggtattt ataagtggag tgatggatgt atatatgcag gtgaatttaa
    21721 aggtggaaag atggaagggt acggtatttg ctataatagt aaaggggaag tactttatga
    21781 aggagaatgg aagaataatt tgattcatgg taagggaact tatatatggg aaaaaggtaa
    21841 aaagtatata ggtgaattta tgcatggtaa aaaacatggg caaggaactt tttatctaaa
    21901 taatgaattg gtttatgaag ggacttggaa atttgataag ccatctatat ttgatagaac
    21961 attagatgaa atattttcct tgagactgta gctttatcta attagtagat aaagcttttt
    22021 tctaatactt actgtaaata ggtatgaatg atattgatta gtatacacta tcaatattaa
    22081 cttaataaat gcaatattaa aaaattttga ttgattttaa gaaggagaga tatattgaag
    22141 agattaattg ctttagtgtt agtagtactt attgttttat tttatagtcc tgaattatta
    22201 aaagaaggat atgatttaaa agaaaagttt tttcaaagtg aaatatggaa tgaattttta
    22261 gacaaaataa atataaatga taattctaag aaagataaaa aagaaaaaag tcaaaattta
    22321 caatcaaata atactgaagg ggagaactta ggagagtcag atataaagaa ttttgaaaaa
    22381 ataagcttag gagataattt aagctatgtt ttatcaagta tcggcaaacc tggtagaatt
    22441 gatattagtg aatatggttt tgattggtat gtatataatc aatatggaaa agaatttgca
    22501 atggttggac ttgaaaatga tgaagtagta gcactttata gtaattctat taattcatgt
    22561 gaaaatcaag atataaaact aaatcaagat agacaaacag tgagaactaa gattacacca
    22621 ttaaaatata agagaaaagg aaataccaga tatataataa actcagaaaa tcaatatgat
    22681 ataataagta aggaaggtaa gtatattaca attttctacg atattcatga agaaaataga
    22741 gtttgttcat atttaataat tgataaaagc actgaagatg agtttaaaaa tttatatcca
    22801 gatgatagcg aagaacttaa aaaatgtttt gaacttgaag ttatagacct tgtaaatagt
    22861 gttagaaatc aaagagggtt aaactcattg agatacagtg aacaggcaac tttgagttca
    22921 agaaaacata gtgaagatat gagagataat aatttttttg accatgtaaa taagaagaat
    22981 gaaactccat ttgacagaat gaaaagagaa ggtatagtat atacttcagc tggagaaaac
    23041 atagcagcag gacagattaa tgcaatatat gcgcatgaag cttggatgaa ctctgaagga
    23101 catagaaaaa atattttggg gaactataat aatattggag ttggagtaat atttggagga
    23161 agctataaaa catattacac tcaaaatttt tataaataac taaaatattt gaaaggaagt
    23221 taaaaaatga catcatggaa aaaaaagaca gtatataagt gcttaatagc agtagtcttg
    23281 ttttgtggaa tagtgttaat atctaatttt agtaaagttt ctgctttgat gatggatact
    23341 aatggaaatg ttttgattaa acatggttca agggaaaaaa agctaatagc aattaccttt
    23401 gatgatggtc ctcatcctaa agaaactagt caagttttag atgtattaaa gaaatacaat
    23461 gtaaaagcta ctttttttat agctggaaaa catgcaaaat ggtataaaga accattagta
    23521 agagcaagta aagaaggtca cgagatagga aatcatactt tcaatcatcc tgatataagt
    23581 aatttaagtt ctagtcagat agaagaggag attgttaagt gtgaagacat tttgaaagaa
    23641 gtaactggaa aaaaaccgac attatttaga ccaccatttg gaagttatag agaaaaagaa
    23701 ttaatagaaa ttgcaaaaaa gcatgactat aaagttgttt tatggacagg tgtagatgtg
    23761 aaagattgga aaaatccagg agctaatagc attgcagata aaataattaa taaagtacaa
    23821 aatggagata taatactttt gcatgattat gcaactaatg atacagttga agctttagat
    23881 atgtttattc caaaaatgat tgaaaaaggt tttaaatttg taacagtttc tgagttaata
    23941 aaataatttt taggggggag atttacagta tgattcttat gatagacaat tatgattctt
    24001 ttgtatacaa cctagtccaa tatatagaag aattagggga aacagttgta gtaaaaagaa
    24061 ataatgaaat caaaataagt gacattgaag aactaaatcc agaggtaatt gtgttatctc
    24121 caggaccgtg ttctccaaaa gaagctggta tttgcataga tattgttgag cattttaagg
    24181 ggaaaaaacc tatactagga atttgtttag gtcaccaaac tattggacat gtttttggag
    24241 gagatattat aaaagcacaa cagcctgtgc atggaaaagt atatagtatc aaccatacta
    24301 ataaaggagt ttttagggga cttaaaaatc ctttaaatgt cactaggtat cattcactaa
    24361 tagttgactc caatacagtt ccaaaagaat tagaaattac agcaattact gataaagggg
    24421 aaataatggg gattaggcat aagaaatatt taatagaagg agtacagttt catccagaag
    24481 ctattttgtc agagtatggt catgaaatgc ttaaaaattt tataacagaa gctagagaaa
    24541 gggtacatat gtaatatgat aagagagata aatactaaat taaattcttt tgagatattt
    24601 actattttta gaaatgaaca tgacagtttt atattagaca gtgctatgga caaagaaaaa
    24661 ttgggaagat attcttttat aagtagccaa ccatttaagg tattaaagta taaagatact
    24721 gatgaaaatc ctttagaagt tttaaaagaa gaactacata agtatagggt tgtaaatgat
    24781 actaatcttc catttgtagg aggtgctgtg ggatatttat cttatgattt aggtaattac
    24841 atagaaaatt tacctagaac tgctgtagat gatattgaga tgcctgatat gtattttgga
    24901 ttttataatc atgtaatagt tatagaccat cttgtgcaaa aaacttatat agctactcca
    24961 aacatagata tcgaattaga agaaaagata atagatgata tagaacaaag aatattaaaa
    25021 gaagagaaaa aaggtataga tagcatatgt tatgaagaaa aagaagtaac acctataaga
    25081 cttaaatcta attttacaaa ggaagaattt aaaaatgcag ttcaaagtgt tagagagtac
    25141 ataagacaag gagatatata tcaagctaat ttaacacaaa ggttcagtgg agaaacagaa
    25201 cttacaagct ttgaattgta tagggactta agaagattta gcccagcacc atttggggca
    25261 ttcttaaact ttgaagatgc acatatatta tcaaattctc cagaaagatt tataagatgt
    25321 gttaataaga gaatagaaac tagaccaata aaaggtactc gtccaagagg gaaagataaa
    25381 gaagaagatt taagacttca acaagaactt agaaatagtg aaaaagatag agctgaacta
    25441 cttatgatag tagatttaga aagaaatgat attgggagaa tatctaaaac tggaagtgtt
    25501 aaagttccag agctatttgt aattgaacca tatgcaaatg taaatcactt agtttccaca
    25561 gttgtaggtg agttaaaaga tgataaagat gctactgatg taataaaagc aacttttcca
    25621 ggggggtcta taacaggagc acctaagatt agagcaatgg aaattataga cgaattagag
    25681 ccaacgcaaa gaaatgtata tactggttca ataggttata taggttttaa tggtgatatg
    25741 gattttaata tagcaattag aacaataatc aaaaatgaca aaaaagtcta ttttcaagtt
    25801 ggaggaggaa tgacttggga ttctgaccca gatgaagaat atcaagaaac acttgataaa
    25861 gctaaatcca taatgaaggc tttgagggga tattatgaag aataatgtag gttttgatag
    25921 tagtttaagt aaatttggaa ttggtttgtt tgaaactatt agagttaaaa atggaatagc
    25981 agtagattta gatatccata tagaaagaat gctaagttca ataaattgtt tggatttaga
    26041 tataaaatat aaaaaagatt tcttgataaa tgaaattgtt acatatataa aaaaagaaaa
    26101 tgtcattaat aaagcactta gaattactgt ttttgatgaa ggttataata tatctattag
    26161 ggatattcca tataatcaag agacgtatga gaaggggttt aaattagcta tatctccaat
    26221 agttaggggg aatagtttaa ttcatagaca taagactact aattattttg aaaacatata
    26281 taccaaaaat atcgcaaata agactggtta taatgatggt atatttttaa attcagaagg
    26341 tgtaatatta gaatgttcta tgagcaatat tttctttgta aaaggagaaa gagtgtacac
    26401 tcctagtgat agattaccga tattaaatgg aataataaaa aaaaggatta tagagatttg
    26461 tgacgaatta catatagagt tgatagaaaa tgaaattaat atatcagaaa ttagtagttt
    26521 tgattttgtt tttgtcacaa atagtcttat gggcgctata aaggttacag aaattgacaa
    26581 aataaaattt aataaagaaa atgttgtttt tgataaaata gtgaagttat tagaataaat
    26641 taaaatatta acaatttgtt ggagacaaaa gatgataggt tgtatttatg aagtatacaa
    26701 tgaagagata actaaaataa tagaaaacca aaacactttg gctgtatatt tagtgggttc
    26761 tagtaaagac gtagatttta ctctttatga tgtagagata aacgatattg atgtgttcgt
    26821 ttttgtaaaa gaaggggaaa aacaagagag aatactgttt aaaaaaaagg gaatagaatt
    26881 tgatgtaaat tacttttcaa aagatggtgt aaaaaaatta ttatcaaata gggaatattt
    26941 ctttgtgaaa gaaatgaaag atgctaaagt actgtttgat agggagaata tatcacatat
    27001 tataaaagat ataagtagaa atatttactt agaaggaccc agtaaaatgt cattagagga
    27061 aaagtctttt ataaagcaag atattggagc aaaaatatcg aatttaaaaa ataaagaaaa
    27121 atttgatgtt tttgaatatc attttttgac gaatttatat ttaaaagata taataattgg
    27181 gtactttaat ataaatgata aatgggtacc aaaagacaaa aaattgctaa aagttttaaa
    27241 aaaagaaaat aatgagcttt ttgaactagt gtcaaaagta agtgaaaatt atgactatca
    27301 aagactttta gatgtttata attatatttt taaagaaatt gagacaaaag aggttataaa
    27361 actaatcttc tagtcacaaa tcaatggggg gagtttaaat gaataaagtt gataaagaaa
    27421 agatacaaca tgcagtaaga gaaatactag aggcaattgg ggaagaccca gatagagaag
    27481 gtctaatcga aactccaaat agagttgcta ggatgtatga agagatattc tctggtctta
    27541 gtgaagaacc aagagaccat ttaaaagttc tttttgcaga tgaaaaacat gaagagttag
    27601 tattggtaaa agatattcca ttttattcat gttgtgagca tcatctagtt cctttctttg
    27661 gaaaagctca tattgcttat ttaccaaaag gaggaagact tacagggctt tctaaacttg
    27721 ctagagtaat agatacattg gcaaaaagac cacaattaca agaaagaatc actaaaaatg
    27781 ctgcagatat aataatggaa gaattacaac cttatggtgt cttagtagta gtagaagctg
    27841 aacatatgtg catgacaatg agaggcgtaa aaaaaccagg ttcaaaaact gttacatcag
    27901 cagtgagagg aatatttgaa aaggacattg cgtctagagc cgaggctatg agtttgatta
    27961 ctatgaagta gcaatttatt atagatataa tcatttattg agataaaatt taatttaagg
    28021 gagtgtcatt aattgtgttt gattatggta aaagaactta tataatggga atattaaatg
    28081 taactccaga tagtttttct gatggaggag attttaataa cttggacata gcaatacaac
    28141 atgcaaaaga tatggttgac caaggtgcag atataataga cctaggagga gagtctacac
    28201 gcccaggtca tagctatgta gactcagacg aagagctaag aagagtaatc cctgtaataa
    28261 aaaaacttaa acaggaattg gatataccaa tatcaataga cacttataag gcagatgttg
    28321 cagaagaagc attaaaatta ggtgtcacta tggtaaatga tgtttggggt cttagaaaag
    28381 ataaaaatat ggcttctgta ataggaaaat atgatgcaga agtgtgtata atgcataatc
    28441 aagatgggac aaactatgac aaagatataa tggagtcaat aaaagacttt tttaaggtaa
    28501 gtatagaaat ggcaatgagt tgtggtgtaa aaaaagaaaa aatagtactt gacccaggtg
    28561 taggatttgg caaggatttt gaacaaaata ttgaagtatt gagaagatta aatgaattaa
    28621 aagatttagg gtatcctatt ttgcttggga catccagaaa atcagtaata ggaaaagtac
    28681 ttccagttga gcctaaaaaa agattagaag gtacaatagc tactactgtt cttggtataa
    28741 gagatggcgt agatatagtt agagtacatg atgtttatga aaatttaatg gcagctagaa
    28801 tgactgatgc aatatataga aagtaggtat aatataatgg ataaaatatt acttagtaat
    28861 ctaggattct atggatatca tggtgttctt aaagaagaaa attttctagg tcagaaattc
    28921 tttgtcgaca tggaacttta tattgattca agagaagcag gtcttagtga cgatataaat
    28981 aaatctgtaa gctatgccga ggtttacaat gttgttaaag atattactga aaataaacaa
    29041 tttaatcttt tggaggcttt agcggaaaat attgctgaag aagtcttgaa taaatttata
    29101 cttataaatg gagttatggt cagagttaga aagcctgagg ctcctgtaaa tggtatttat
    29161 gactattttg gtgttgagat aaggagaaca agagatgaat aaggcttatt tgggaatagg
    29221 tactaacatg ggtgatagat ttgataattt atcaagagca tgtgaacttt tgaaaaatag
    29281 tgattctata tataaagtaa aagaatcaag cttatacgaa acaaaaccat ggggatatac
    29341 agaacaagca gattttctaa atatgtgtgt agaaatagaa acagagtttg aaccttatga
    29401 gttgttagaa tattgtcaag aaatagagag agaactacac agagagagaa tagttcattg
    29461 gggaccaaga actatagatg tagacgtact attttttaat gatgtagttt ccactgatga
    29521 aagactaaca atacctcatc caaggattca agatagagct ttcgttttga taccacttat
    29581 ggatttaaat gaagaactta taataaatga aaaaacaata aaagaacatt taaacctcct
    29641 atctgctgaa gaaagagaag aagtaaagga gcttgtaggt tatgaaagaa aacctattta
    29701 ataataattc aaagatagag ataaatacaa ataacaaaaa gataactgta aaaaaagata
    29761 aacttataat agctggacca tgcgcaatag agagttatga gcaacttttg gagactgcta
    29821 aatttgtaaa aagtcaagga gcaaacattt taagaggtgg agcatacaag ccaagaacat
    29881 cgccaaattc atttcaagga ttaaaaaaag aaggtcttga aatattaaaa gcagttaaag
    29941 acgaagttgg tatggctgtt attacagaac ttatggatgt aagagatatg gacgaactgt
    30001 atagtataag tgatataatt caaattgggt caagaaatat gcaaaacttt actcttttaa
    30061 gtgaagtggg aaaacaaaat aaacctgtta tgttaaaaag aggaatagca tccacaatta
    30121 ctgaatggat aggtgcttct gaatatatag caatagaagg aaatagtaat attataatgt
    30181 gtgagagagg tataagaacg tataatgatt atactagaaa tacattagat ttagctgcgg
    30241 tacccattat tcaaaaggaa acaggtcttc ctgttgtggt agacccaagc catgccactg
    30301 gagttagata tttggtaaaa cctatgtcac tggcatcttt tgcatgtgga gcagatggaa
    30361 taatggtaga agtacatcct gacccagaaa atgctttgtc agatggtgct caatctcttt
    30421 gctttaatga atttgaagat ttaatgaaat caattaataa ttattaaaag aggcaagagc
    30481 ctctttttta ttcgtaatcg ttgctaaggt aaatttataa gttataattt gatagttaga
    30541 ttatttaata taaataaaga tttaatattg atagaattaa tataaaaaat aaaaattttt
    30601 tgattaataa gaaggatatc aataaaaaaa gtcgaattgt gtatattgag tgattgacaa
    30661 atttagaata tagtgtagaa tagaaatgtt aacacaaaaa attacaccag aaggtgataa
    30721 tatgaatgat ataaaagaca ttattgaaga aataaaagct agatgcgata tagcaagtat
    30781 aatatctgaa tatatgaata taaaacaatc tggagctaat tacaaaggat tatgtccatt
    30841 tcatggcgaa aagacacctt ctttttatat aaatacatca aaacagattt ataagtgttt
    30901 tggttgtggt gaaggtggag acgtaataaa ttttgtcatg aaaatggaaa atttggattt
    30961 tatggatgct gtaaaaattt tagcaaataa atgcggtata gaaattaaca ctaacatgaa
    31021 tgaagaaact aggattaaaa tagaaaaatc taagaaattt caagatattc atacggaagc
    31081 agctagattt tatctttcaa acttattggg aagcaaaaat cttgggtatg aatatttaag
    31141 aataagagga ctagatgata aaattattaa gaaatttggt ttaggatttt ctttggattc
    31201 atggaattca cttatgaata ctctaatcag taaaggatat aaaaaacaag acttattaga
    31261 gtgcggtcta attgcaaaaa atagagatgg aactaattgc tatgataaat ttagaaatag
    31321 agttatgttt cctatattcg attatagagg aaatataata ggatttgggg gaagagtatt
    31381 agacgattct ttgccaaaat acttaaattc tccagacaca ttaattttta ataaaaaaca
    31441 gaacttatac ggattaaatt ttgctagaaa aaatttagaa agtaaaacta tagtcttagt
    31501 tgaaggatat atggatttaa tttcacttta tcagtatggt ataaaaaatg tagtagcaac
    31561 ccttggaaca gccttgactg aacaacaagg cattttaata aagagatatg cggacactgc
    31621 tattatatct tatgattctg atgaagcagg aataaaagcg actttaagag ctatagatat
    31681 acttactaaa ttaggtatca atgtaaaagt gttagactta aaagatgcta aagacccaga
    31741 tgaatttgta agaaaatacg gacttagtga ttataaaaaa gctatggatg tttctactca
    31801 ctacataaaa tataaaatag atcatcttaa aaaagaattt aacattcaaa aagatgaaga
    31861 acgagtgaag ttcgcaaaag aagcttctaa aattataaaa caattgacaa gtcctgttga
    31921 aattgatttt tatacaaagt atttaagcaa tcaaatagat attaatgtcg agtctataaa
    31981 gagagaagtt tatggtaaaa attacaataa gccatataat aacaaaaatc aaaagaaaat
    32041 agaagaaaaa gtaattgaaa aagtcgaagt cagacaagac ggaaaacaat tagttgaaga
    32101 aactctaata aaaataatgt tagaagacaa aaaaattaga gaaatagcgt tacttaaagt
    32161 agaagaaagt gattttttat tgaaagaaag taaagaaatt ttaaattaca tgattaaaaa
    32221 tcaagaattg gacaaaataa ctattgacaa attgaaaagt ttaaacatat ccgaagaata
    32281 cttaaaagaa ttaaattcaa tttctttgaa tagtataaac ttagagaaca caaaagaaat
    32341 agaaggaata ataacaaata tcagaaaaaa ctcattagaa gagcaaatta atagcttgtt
    32401 gagagaacaa caagaattag aaaataataa tgatatgaaa gaggtagatg gtagagttat
    32461 ggaaattgct ttaaaaatag ttgagataaa taaaatttta aagagcttgt agagaaagga
    32521 ggtttgagtg tggaaaataa atcgaataaa aaagagttaa aaaaggttac tgctaagaca
    32581 ttaatagaaa aaggaaaaaa gcaaggttct ctgacacttg cagagataat ggaagctttt
    32641 tcggagactg aacttgataa ggatcaagta gaaaatcttt atgagacttt aggaaatttg
    32701 ggaatagaaa taacagaaac aaaaaactat aaagctgata tagatttttc ggttgctgat
    32761 gacgatttaa gtataggcca cttagatgaa gatgcagagg caatttcaca tgatgactct
    32821 tctgcaatag aaatagaaac tgtggattta tctttaccaa aagggataag tatagatgac
    32881 cctgtaagaa tgtacttaaa agaaatagga aaaattcctc tacttaaacc acatgaagaa
    32941 gtagaatttg ctagaaggat gcacgaaggt gatgagatag caaaacaaag gttagttgaa
    33001 gcaaacttaa gactagttgt aagtatagca aaaagatatg taggaagagg tatgcttttc
    33061 ttggatttaa tacaagaggg aaatttaggt cttataaaag cagtcgaaaa atttgactat
    33121 acaaaaggat ataagtttag tacttatgca acatggtgga taagacaagc tataacacgt
    33181 gctattgcag accaagctag aactataaga ataccagttc atatggtaga gactataaat
    33241 aagctaataa gagtatcaag acaactactt caagaacttg gaagagatcc aaaaccagaa
    33301 gaaattgcaa aagaaatgga aatgacagaa gataaagtaa gagaaataat gaaaatagct
    33361 caagaccctg tgtctttaga aacaccaata ggagaagaag aagatagcca tttaggtgac
    33421 tttattccag atgatgatgc tccagcccca gcagaggcag cagcatattc tttattaaaa
    33481 gaacaaatag aagatgtact tggttcatta aatgatagag aacaaaaagt attaaagctt
    33541 agatttggtc ttgaggatgg tagagccaga actcttgagg aagttggaaa agagtttgat
    33601 gtaactagag agagaataag acagatagaa gcaaaagcac taagaaaact aagacatcca
    33661 agtagatcaa aaaaacttag agattattta gactaaaaag atacatcatt gatgtttttg
    33721 tgataaatgc catgagataa gtgatatctc atggctattt tgtagtaaag atttaatttt
    33781 atttttgtag ttatttcagt ctaatatcta taacttggat taaatcctat aaataaaggg
    33841 aattgaaagg agaattatat ttgaaattaa cagatagatt attgaaaata gcatcattag
    33901 tgtcagatgg taaaaaaata gctgatatag gaacggacca cggatacata ccagtatatt
    33961 tgttaaaaga gggaaaagta ccatttgcag tgctagcgga tgttaataaa ggacctcttg
    34021 acaatgcaca taaagaagtc atacagaaca atcttttaga taaagttgat ttacgattgg
    34081 gttctggtat agagatactt gaaatagggg aagtagaaga agtgataatt gctggtatgg
    34141 gtggaatctt gataagtgag cttctcgaag ctaaaaaaga agtagcacat aatgtagaaa
    34201 aattaatatt acagccaatg caagcacaag aagaacttag atattacctt ttaaacaacg
    34261 gatatgaaat tttagaagaa gttttagtga gagaagattt tagaatatat gagataattg
    34321 ttgccaaata tacaggtaaa aatactataa tagaagatga gatatattat gaggttggaa
    34381 taaagctttt agaaaacaaa gattctcttt ttaatgattt tatagaaaaa aagataaaga
    34441 catatagttc tatagttaat aaattagaag ggaaaaatgg ggaagctata gataagaaga
    34501 gaaaggaaag cgaagttgca ataaaaaaac ttgaaaattt aataaaataa ataggaggta
    34561 tatatgctat taaagagtct aactagaaaa attgaaaaaa agtatcctct aaacttagca
    34621 gaggattggg ataatgtagg tcttatagtt ggagattttg atatggatgt aaaaaaggta
    34681 ttggtatctt tagaagctaa tgaagatgtt attgatgaag ctatatccaa aaatatagat
    34741 ttaattgtga cacatcatcc ttttatattt gggaaaatta ataaaattaa tagtggagat
    34801 ttaaaaggca gacttatcca aaaacttatt aaaaatgata tatcacttta ttctatgcat
    34861 acgaattttg atatagcatt tgatggtcta aatgactatt ttatggaaat tatggaattt
    34921 ggtaattcaa aggttttaga tataactaaa agcgaaaatt tgtataaatt agcagtatac
    34981 gttccacata actattcaga tgaacttaga aaggttttaa gtaattcagg agcaggtcat
    35041 atagggaatt atagtgattg tacgttttca acagaaggtg aaggacaatt taagccatta
    35101 gaaggctcta atccgtttct tggaagtgta aatgatatag aaactgtaaa tgaagttaaa
    35161 attgaaactg tagtaccaca gaagctttta ggtggggtaa taagttctat gttagatgca
    35221 catccttatg aagaagttgc atatgatttg tacaagttag aaaataaagg tgagatattt
    35281 ggactaggta gaataagtaa acttgataaa agtatgacat tagaaagtct ttcaaggaaa
    35341 ataaaagaga aattaaatat gaaacacata agagtagttg gaaaccttag tacagacata
    35401 acgaaagtag ccgttgtaac aggagctggt tcagaatttg taaaaaaagc taagagacaa
    35461 ggtgcagaag tactaattac gggtgatgta aagtatcatg aggcacaaga tgcacttgat
    35521 attggaatgt gtatagtgga ttgtggtcat tttgatacag aagatatatt caaaaatgtt
    35581 atgaagagat ttttagatga atttagtgaa attgaagtaa taaagagtaa tgtatatttg
    35641 aatccattta gtactattta gcagtgtttg ataataaaaa tactatataa aaaataaatt
    35701 tgagtttaat tagggggata atgatgaaag tagcagtaat ttttcatagt gtttgtggga
    35761 gtacatattt attagctaga gaatataaag aagcgcttga agaaatgaat atcgaggtag
    35821 atatatttag agtaagtgac gaagttgcaa aaacacttcc tcaatattat ttgataaatt
    35881 ccaaagaata caaagacgaa tttgaaagta taaatgtaat taaatctgga aaagaaatat
    35941 tagattatga tgctatattt atgggttcac caacatatta tggaaatgta tctggaccaa
    36001 tgaagatgtt tatggatagt ttttctgata tttgggtagg tgctcctttg agtggtaaaa
    36061 tatttggttg ttttgctaca gcaggttcac aacatggagg aggagaatta gctttgcaag
    36121 ctatgaatat atttgcccaa catatgggaa tgactctttt gtcagttcca tgttcagtaa
    36181 gaggaggtta tccagcgtat ggaatacttc atatagcagg agataattct gatataaggc
    36241 caaatgatga tgccaagata ggtataagag attacctaaa aagacttaac atataagaat
    36301 atcatataaa aatacctaaa agcgactgta tatactttta ggtattttta cttaattata
    36361 aacgtgaagc tttaccagaa atatggtcaa tttctatttt aacaacagtt gttaagttca
    36421 tatccttctc tatgtacttc atgccttctt caaagaatcc tttagaatat ttttttatta
    36481 tttctacaag agcatttttc ttttcttcat tttctacagt gcaagctttt ccaaaaacta
    36541 tagcactaga ataagtagta ctaaatttat ctggaataac actttcatta gcaactacta
    36601 aaaaagaaac tttgttgttt ttcgatatat tatctaactt atgcccattt cttgcacaat
    36661 gaaagtaaat agaatcatta aagtatacat aatttacagc aactccataa ggataaccat
    36721 tttcagatat agtggaaaaa gtaccaaatt caccattttt taatacttca acagtatctt
    36781 cttttgtcat ttctcttttt ttcaatctca tttctttaaa cataatatca tcctttctaa
    36841 ttttgatgct taaattaaat tttagaacat ttataaagat tatgctatgt tttttacaaa
    36901 ttttaagaaa aaaatatagt ttaagataaa aggtagtgaa agagaagcta tttggaggtt
    36961 tatttatgat attattgtta gagtagtaca aataatacta ataaatttaa tagctggttt
    37021 cttgttattt aaggtagtaa gcatgtctat aagaagggga agttatcgag ggtagaagtt
    37081 gttgtttata acgatattat aaagaaaata ttattatata aaataataaa tagtaaagag
    37141 gtatcaagtt gaatataata cttaatacct cttcaaaatt aagttataaa agtgttgtgg
    37201 gaattgtcct atacatggta aggagataat aaagaatgta tctttaaagt ctcaactaaa
    37261 cagcaaaatt tatccaacaa acatacaata aatgcttctt tgtatctagg tggcttaaca
    37321 gtaagtggcc acatgtgttt taaaattatg tccgtttctt tttcatttat ttgaaaaaat
    37381 aaagttgcat tttttaatgc ttctctaggg tgagtaaatc catgtaatcc ttttctatca
    37441 cctttgtaat gccaatcata aagaaaaaag tcgtgtagta aagctcctct aataacactt
    37501 tgaatatcaa cacttaagtg caatttttta cataatagat aactataata tgctactgac
    37561 aaactatgtt caagacatga aacattgcca tgatgaggaa atgttctcat aagttgaact
    37621 ttttcagaag aaagtatctc agaagcacat tcattaaaat atttgatttc agcttttgac
    37681 aacattatga aaatctccat tcttagtaat atataaatac tatacttcaa cgataatatg
    37741 aaatatttct tatcctacaa aatataatac ccaataatac aagtaagata ccttttatgg
    37801 tattttaaag taagagagat tttattaggc taaacaatga aaaaattaaa agtaaagcta
    37861 tagcaatata tagtattttt ccaagaggat tgccaaaatt tatagtccat ccaataccaa
    37921 accttttatt aaccattaat gatgggtcat ttggattatt atacatgatg cctgctatcc
    37981 aatacttctc atcttcttct ggagaataag atgagttgaa ttttaaattt ggggaattaa
    38041 tatatgttgc tattaaataa attataacta aaagcgttgt aatattcata gcagttaata
    38101 gttttgtttt tattgataaa agagtagtaa aaaactgtgt tgtcattata atcatcaata
    38161 ttaaaaacaa ataaccaatt ttatttaaat atttaatgtt ttcaactcta ctttcttcaa
    38221 ttctatttgt atctatctta atccttgact ttatggatcc aatggaagtt atatataata
    38281 aaaccattaa acaaaagtca gtaccgatta atttaaatac attaagaaat gatttatcta
    38341 taaaaacatc aggagaccca tttatgttcc aatgagtggc aattgagtct ggcaattgat
    38401 tataattaaa tgctataaaa atactcattc ccaaagtaaa tagtatcggt attaaataga
    38461 ttattttaaa tttttttatt attttatttt tcttatttat aaaatccata tctactatta
    38521 attttgaatc tatatctata tgacctaatt tagcataaat ttctgattta agcttcttag
    38581 cttttttatg tgtatgaata tatagtatac tttcatataa taaaaaacct ataataccaa
    38641 taaaatagga aagttccaat ttattaaatg taaagaccag aataaaaact aatataaagt
    38701 ctataataaa acctaaagat aatagttttt taaattcttt atctaacttt ataaagtaat
    38761 tataattttt atgctcttgg tctatagaaa ctccatagat gtgtttttta cctacaaaat
    38821 agggtataaa ataaaatgta gcatacatca aaattagaac tggaaatatt gtaaatagac
    38881 attcaatctt actcataata tttcacctca tacttgtcaa ataaacttga acttagtgac
    38941 aaaaattctt ctttactaat acctgataaa taagattttg ccaccaataa ttccaagtca
    39001 agtttatttt tttgacgagt tgtattatct attttcattg gtagttgagc tacaatagca
    39061 ccttttcttc tatcaatatt taagaatccc tcttctttta attcattgta ggatttgttc
    39121 actgtatgta aatttacacc aatattttca gccatacttc ttacagatgg aagtgattca
    39181 tttattttca attctcctga agctatagcc ttagttattt cttctttaat ttgtacataa
    39241 atagatttat cactattaaa atctaaattt aaaaccataa aaaacctcca ttatgcttta
    39301 taaatgaaaa cgattgttat atacatagta taacataaaa aataataaca aacacaaaat
    39361 ataagtttat attttgtaaa aattgtaatt taaaaaataa cataattttt attaaaataa
    39421 tacagatact atttatagat agaaataaaa attagaaagg agaaaaaata tgttaacata
    39481 tttaatgaaa agacaaatgc ataaaaaaga tgatggaatg agtaaaccaa tgatgattac
    39541 aggagctgta attacaggtg taggagcata tgctgtatat aaaatggtaa aaagccataa
    39601 atctaattct caaatggtca atacaaatta ttatggaaat agcgattatg atgattataa
    39661 atatgacgaa tttgattatg actgtgattg tagagacaca aaacaagatt atgaatatga
    39721 agaattaaaa aaaaaagtaa aagaatttaa ttcaagaaga ataaattgtg aaaattgtcc
    39781 acatgtttct caagaagaaa tgatgcaata tgtaaatagt gttaaagatg taaaggaaga
    39841 gcttgattta catgaagatg ctaaaaaaga taatatttac aaagaagatg aatacaaaaa
    39901 atatgaagaa gaataagtaa tattttgtat aactgaacaa atagtaatgt caaattattt
    39961 aatatctagt tttaatatgt aagttgtaac ttaacttgaa agaatcagta tcattcttta
    40021 gatagttaag taacaactta cattttattt ttataaattt taatagttct ttgtgactat
    40081 tttgtaattt tagaaatatt ttagtatcca gggtaattgt gtataataaa acttgtatag
    40141 atagatagta tgaaattaag cagatagttt aaaattaaaa tgaaaagaaa ggaaatagtt
    40201 atgttaaagt tgttgatagt tgaagatgat agtactatat cttttggaat aaagtatgct
    40261 ttagaacaag aaggatttag tatagatata tcaaaggatt tatctagtgg aaaagaaatg
    40321 atttcttcta atgaatatag catgatactg ttggatgtta cattgccaga tggaactggt
    40381 tatgagcttt gtcaatatat aagaggattc tctcaggttc ctataatatt cttgactgct
    40441 tgtgatgaag aagtaaatat tgtaatgggt ctcgatatag gtggagatga ttatataaca
    40501 aaacctttta gaattagaga attaatatcc agaataaaag ctgttttaag aagaaaaggt
    40561 aatacttcag aagaaaataa gaaaatactt aaatttggag atttaagtat atatacatta
    40621 gaagctagag tatataaaaa tgataaagaa atatttctga catcagttga atataaactt
    40681 cttttaattc ttatacagaa taaaaatact gttttaagta gaactcaaat attggaaaaa
    40741 ctttgggatg taacttatga ttttgtaaac gacaatactc taactgttta tataaaaagg
    40801 cttagagaaa aaataggtga tgatttatgt gaaccaatct acatagaaac tgtaaggggg
    40861 attggttata aatggatagg aagtgaaaat agtgtttcta tataatccag aagtgaagaa
    40921 gtttttaagt aagtatgtta ctttgatgtt tattgttatc ataatatcta ttggatttag
    40981 cataatcaac gtgtccttaa ctaaagagat ggttgtaaga aataatcagg ctataatagg
    41041 aactttatca agcaagtatc caaacttaga aagtcagata gtagacataa taactcaagg
    41101 gaagtctatg gagaacatag attatggtca gaaaatatta attaagtata attatgataa
    41161 atctattagg ataaactctg agcctattat ttctaaatta gttttagaga ctatcaaaat
    41221 aaatataata ttagtatgta taatatttat tttgatattt gtgttgattg ttagatattt
    41281 tacgtcaatt tacaatgatt tatcagatat gactaagtat gtttactata gttcagaggg
    41341 taagccattt gatatgaaaa acaagaatca agaagggcag ataggtcttc taaaaactga
    41401 actcttaaaa atgacgacaa ttcttaatga aaaggttgaa cttttaaaaa gagaaaagat
    41461 atttttaaac aatacaattt ccgatatatc acatcaatta aaaactccta tgacttcttt
    41521 aataatgttg aatgatttat tatataatga tgtaccatac gaagtaaaaa tcgatttttt
    41581 aaataagata aaaaatcaat taaatagaat ggattggttg attaaaagca tgcttaaatt
    41641 atcaaaggta gaagcaaaag tgataaattt taaaaaagat aaggtaaaat ttagtgaact
    41701 catacataga gcaatgctat ctatgaaaat acctatggag attaaaaatc aaaaactgac
    41761 tatagaggga aatgataatg tatcttatat tggtgatatt gattggtccg tggaagcatt
    41821 agtgaatata atcaaaaatt gtgtagaaca tactcctgaa tttggaaata taattataac
    41881 ctataaagaa aatccattat tttctgaact aataataaaa gatgagggtg aaggtataga
    41941 taaaaaagat ataccacatg tatttaaacg attctataga ggaagaagta gttcaaaaga
    42001 agatagtgta ggaataggtc ttgcaatgtc aaaatctata atagaaagcc aaaatggcga
    42061 tatatatgta aacagcgaaa aaggtaaggg tacagaattt catataatat ttcataaaat
    42121 gtatgatagt gactaatctg taatatttaa ttcactttat agtaatagcc aaaacctaat
    42181 atatgaagta tagaatataa atattggagg tagttatgga aattttaaga gtagaaaatc
    42241 taactaaaag ttatggtaaa aatgaaacaa aagttgatgc tataaaaaat gtaagtttat
    42301 cagttgaaaa aggaacgttt atagcaataa caggtccaag tggaagtggt aaaagtacct
    42361 tattacattt attaggagga gttgacagac caactagtgg taaggtttat ataaatgatg
    42421 ttgatatata taatttaaaa actaaggatt tggcgatatt tagaagaaga aatataggtc
    42481 ttatatatca attttacaac ttaattccag tcttaaatgt gaaggaaaac atattgcttc
    42541 cagcagagct tgacaataga gatattgata gagaatattt tgatgattta atgaaaacat
    42601 taggtctaat tgatagagaa aagcaccttc caaaccaagt atcaggaggt caacaacaga
    42661 gaacctctat aggacgtgca ttaattaata gaccttctat aatattagca gatgagccaa
    42721 caggtaattt agatagtaaa aattcaaagg aaatattaga attgttaaaa ttgtcagtaa
    42781 aaaaatacaa ccagacacta ataatgataa ctcatgacaa aaatatggca cttcaagctg
    42841 atagaattat aagcatagaa gatggaatca taaaaagtga tgaggtgatg tagatgaatt
    42901 tatatacatc actaactatt agatacttaa aacaaaataa aagaagaacc attgtaacca
    42961 taatcggtat aatactttca acagctttaa tttgtggtat aggaaatata tttgaaagtt
    43021 ttatggatta tcaaatgaga gaaactataa agaatgatgg aagtttccat gttacatttt
    43081 atgatgtaaa tagaaaaaat gtggaatatg taacgaaatc agctgaaata gaaaaacatg
    43141 catttaccaa acaacttgga tattctaaat tagaaaatag tgaaaatgct atattaagta
    43201 taaagcaata tgataaaaat gcaataaatg gatataaagt ttctataaaa gaaggtagat
    43261 ttccagctaa ggaaggagaa attgttttaa gtgaaagtat tattaatctt atagatgata
    43321 agttaaaaat aggagataag ataactttaa aagtagggga tatgtttgac tcaagcaaga
    43381 aaaagattga tagtatgaca tttcatgaag gtgattatat agcaaatgaa aaagatagaa
    43441 cttttaaagt tgttggtata attgaaaaac ctgggtttga acgttataat ggaattgcaa
    43501 cagctatagc ctactttaat cctatgaata attacaatga tacaattaat gtatcagtag
    43561 cagtaaaaaa tcctaaagat gtgtataaga taagtaataa aataagtaaa aatatatatt
    43621 caaataaaga taaagaagca gtttctgata tgataaaata caatgaacat ttattgaggt
    43681 tacaaggtgc tagtaagtat tcaaatataa atagctctat aaagtctata attatagcag
    43741 taactatttt agtaatcgta tgtacaattg ctacagtgta caattccttt agtatttcta
    43801 taaatgaacg aaaaaaacag tttgggatat taaattctat aggtgctaca tctagtcaaa
    43861 ttaaaagatt agtttttata gaaggtatta taattagttt aattggtata ccaataggac
    43921 taatatctgg aacagtagct atagacttat tgtttaaaat tataaataag tattttactg
    43981 aatcggttgt tactaagatg agtttgcaga tagtgtataa tccaataata attattgtta
    44041 gtataataat agttttgctt acaatcttta tatcaattct actacctgca atatcggctt
    44101 ctaatatttc tccccttaat gtgattaaaa atactggaga gtataaagtg ggcaaaatca
    44161 aaagctcaag acttattaaa atgattttta aaactgaagg agtgcttgca tataaaaata
    44221 ttaggagaaa tagaaaaaaa tttataataa ctcttttttc tttaatggta agtataataa
    44281 tatttatatc ttttagtggc tttacattac ttttactaag aggtgaagaa attagaaatt
    44341 ctcaaagaaa atatgatttg tatttaactg caaagaacac agctaggtct gtagaggata
    44401 ctataagtga actagaagat attgatggga taaaaaactt tgaattggca acaggacaat
    44461 atgtgtctat aagagtaagt gaaaataaaa taaataaatc caaggaagat ttaatcagga
    44521 aatattatca aaaatataag attggagata gttatgaata tgatttcagt aataatgaat
    44581 taatttttcc aggagatttt gcagtaaaaa acaaaataaa taacttagta cagggctctt
    44641 ttaataaaga gagagcaatt gaagaaaatg gtgttatatt agttagaaaa agtgcaattg
    44701 aagaaaatgg gaagaaaggt gtagtagaac ttaccaacta taaagtagga gatacagtaa
    44761 attgtgaata tcttgatgaa aatgccttaa gtaaaaaagt aaaggtcaag attcttgcta
    44821 taactgacga agaacgtcta ggtcttggtt atcaaaatat ggggttacaa tttataactt
    44881 atgatgaagt tgctaaaaat ttaaatttaa aattaagtaa aagccttata tttattgatt
    44941 caggaggaag cattaagact aagaaggaaa tagaagcttt ggcaaataaa aataatttta
    45001 acttttatga tgaaagtgca agcaatgaaa gtgaaaaaca agatttaaag gttattaaaa
    45061 tatttgtata tggttttata gtagtgatat ctttagtgag tgttacaaat attctaaata
    45121 cagtaagtac aagtataaat ctaagaaaaa gagaacttgc agtaatacaa tctatagggg
    45181 ttactcctaa aggatttagg aaaatgatat atttagaaag ttttatatat ggtatactgt
    45241 cattgttgtt tggaatacca attagtattg gtataattct aattatgaat aaacttattt
    45301 caggtgtaat tgaattttct ccgattatac cgtggacagc catagttatc tgtatagtaa
    45361 gtgtttttat aataactttt atagcagggt atataccaat gagtaaatta aataaagaaa
    45421 atataataga taatata
//
LOCUS       DAMJZE010000014        44055 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJZE010000014 DAMJZE010000000
VERSION     DAMJZE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.014"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(121..894)
                     /locus_tag="PC541_000678"
     CDS             complement(121..894)
                     /locus_tag="PC541_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2838156.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC541_000679"
     CDS             complement(894..1280)
                     /locus_tag="PC541_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2838157.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC541_000680"
     CDS             complement(1305..1622)
                     /locus_tag="PC541_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2838158.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC541_000681"
     CDS             complement(1634..1894)
                     /locus_tag="PC541_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2838159.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC541_000682"
     CDS             complement(1894..2190)
                     /locus_tag="PC541_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2838160.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC541_000683"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC541_000683"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2838161.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC541_000684"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC541_000684"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2838162.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC541_000685"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC541_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2838163.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC541_000686"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC541_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2838164.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="PC541_000687"
     CDS             complement(8083..9189)
                     /locus_tag="PC541_000687"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2838165.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC541_000688"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC541_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2838166.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="PC541_000689"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="PC541_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2838167.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="PC541_000690"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="PC541_000690"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2838168.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="PC541_000691"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="PC541_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2838169.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="PC541_000692"
     CDS             12283..12990
                     /locus_tag="PC541_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2838170.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="PC541_000693"
     CDS             12959..13588
                     /locus_tag="PC541_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2838171.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="PC541_000694"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="PC541_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2838172.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="PC541_000695"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="PC541_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2838173.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="PC541_000696"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="PC541_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2838174.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="PC541_000697"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="PC541_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2838175.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="PC541_000698"
     CDS             18864..19637
                     /locus_tag="PC541_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2838176.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="PC541_000699"
     CDS             19639..20502
                     /locus_tag="PC541_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2838177.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="PC541_000700"
     CDS             20535..21677
                     /locus_tag="PC541_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2838178.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21811..22386)
                     /locus_tag="PC541_000701"
     CDS             complement(21811..22386)
                     /locus_tag="PC541_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2838179.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22646..23938
                     /locus_tag="PC541_000702"
     CDS             22646..23938
                     /locus_tag="PC541_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2838180.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24043..24645
                     /gene="yedF"
                     /locus_tag="PC541_000703"
     CDS             24043..24645
                     /gene="yedF"
                     /locus_tag="PC541_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2838181.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24718..24981
                     /locus_tag="PC541_000704"
     CDS             24718..24981
                     /locus_tag="PC541_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2838182.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24996..25181
                     /locus_tag="PC541_000705"
     CDS             24996..25181
                     /locus_tag="PC541_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2838183.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25284..26480
                     /locus_tag="PC541_000706"
     CDS             25284..26480
                     /locus_tag="PC541_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2838184.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26694..26972
                     /locus_tag="PC541_000707"
     CDS             26694..26972
                     /locus_tag="PC541_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2838185.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27009..27443
                     /gene="ssb"
                     /locus_tag="PC541_000708"
     CDS             27009..27443
                     /gene="ssb"
                     /locus_tag="PC541_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2838186.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27462..27689
                     /locus_tag="PC541_000709"
     CDS             27462..27689
                     /locus_tag="PC541_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2838187.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27832..28152
                     /locus_tag="PC541_000710"
     CDS             27832..28152
                     /locus_tag="PC541_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2838188.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28160..29116
                     /locus_tag="PC541_000711"
     CDS             28160..29116
                     /locus_tag="PC541_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2838189.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29127..31121
                     /locus_tag="PC541_000712"
     CDS             29127..31121
                     /locus_tag="PC541_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2838190.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31122..31571
                     /locus_tag="PC541_000713"
     CDS             31122..31571
                     /locus_tag="PC541_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2838191.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31590..32918
                     /gene="dnaB"
                     /locus_tag="PC541_000714"
     CDS             31590..32918
                     /gene="dnaB"
                     /locus_tag="PC541_000714"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2838192.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33123..33431
                     /locus_tag="PC541_000715"
     CDS             33123..33431
                     /locus_tag="PC541_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838193.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33513..34802
                     /locus_tag="PC541_000716"
     CDS             33513..34802
                     /locus_tag="PC541_000716"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2838194.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35128..35203
                     /locus_tag="PC541_000717"
     tRNA            35128..35203
                     /locus_tag="PC541_000717"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35161..35163,aa:Lys,seq:ttt)
     gene            35210..35284
                     /locus_tag="PC541_000718"
     tRNA            35210..35284
                     /locus_tag="PC541_000718"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35243..35245,aa:Glu,seq:ttc)
     gene            35289..35364
                     /locus_tag="PC541_000719"
     tRNA            35289..35364
                     /locus_tag="PC541_000719"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35322..35324,aa:Val,seq:tac)
     gene            35398..35472
                     /locus_tag="PC541_000720"
     tRNA            35398..35472
                     /locus_tag="PC541_000720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35430..35432,aa:Thr,seq:tgt)
     gene            complement(35855..36847)
                     /locus_tag="PC541_000721"
     CDS             complement(35855..36847)
                     /locus_tag="PC541_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2838195.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36856..37914)
                     /locus_tag="PC541_000722"
     CDS             complement(36856..37914)
                     /locus_tag="PC541_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2838196.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38039..39502
                     /locus_tag="PC541_000723"
     CDS             38039..39502
                     /locus_tag="PC541_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2838197.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39604..40401
                     /locus_tag="PC541_000724"
     CDS             39604..40401
                     /locus_tag="PC541_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2838198.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40414..42516
                     /locus_tag="PC541_000725"
     CDS             40414..42516
                     /locus_tag="PC541_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2838199.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42584..43063
                     /gene="rlmH"
                     /locus_tag="PC541_000726"
     CDS             42584..43063
                     /gene="rlmH"
                     /locus_tag="PC541_000726"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2838200.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43212..43523
                     /locus_tag="PC541_000727"
     CDS             43212..43523
                     /locus_tag="PC541_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838201.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43571..43888
                     /locus_tag="PC541_000728"
     CDS             43571..43888
                     /locus_tag="PC541_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2838202.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT        16621 a         5540 c         6997 g        14897 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctagtaagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcat taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctatta ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggctg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaagatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttta
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaagg ggatatttca
    21721 aaataaaaat ttgaatcatc tccttttttg tacaaaaaag aaaaggggtt cctaagaacc
    21781 tctaatctat ttgataatag tttcaagaaa ttactttaag taatctcctt cttcaagaac
    21841 tatatgacca ctttcgtaat tagaaccatc aacagtaatt tttatttgtt caacattcat
    21901 attttttatg taagtacgag caactgaatc aagcattaaa gtttctgcat cactacctaa
    21961 gtttgacttt gtaaagtcag agctaacatc taatatacca gtttttacac catcaatgtc
    22021 ttttgtagta aatgaattta attttgcaga ttctggaaca actttaagtt tttgaagttc
    22081 ttcaaataaa gtgttctcat caattttatt taaatcaact ttattttcaa ttaaatctgt
    22141 cttatctaca tcaaaagagt aaatagtagc tgattgagtt ttagtttctt cttttggagt
    22201 aggtttagct ttgttattag gaacctcttt ttcagtattg ttttcttctt ttggttgctc
    22261 aacatttgta ttgctcttag tttctttttt agatgaatca cttccatttt gagcattagt
    22321 acaaccaact gcgaatatag atatagataa tatacttaac actaacatta ttttcttatt
    22381 tttcataaaa atttctcctc tcttaaaaac tattcttata tataattata gtcgttatga
    22441 gcaaatatat gaatacaaaa tagtaacaaa tttaaaaaat gatttattta aattaataaa
    22501 actcaaaata aactaaattt tagtcatttt tatgggaaat catttaatgt acagtgaaaa
    22561 tattgtaaaa taaaagtagt gtttttaaaa taaatataag ttataattat attataaaaa
    22621 aatgtcttca aaaggtggga atagtatgga aattttgagc ttaggagaaa agattaaaaa
    22681 attaagaaaa gaaaaaaaca tgacattaaa agaattagct ggagatagga taacagcagc
    22741 acaaataagc catatagaga gggataaatc tcatactagt tatgaactgt tggaatatct
    22801 aggcgataag ctagatgtaa gtgtagaata cttattagag accaaggaaa tgcaatcaaa
    22861 aaagataact gataatttaa tactacagag cgaaatttat ataaaagaaa atgaattaaa
    22921 caaggctgag gaccaaataa aagaagtttt agatatatgt aagagatacg atttaatgga
    22981 gaactatggc agatgtaatt ttttaatggc taatattaat ttgaaaaaat ctaattataa
    23041 tgatgctata gaaaactttg aaaaggcatt gtacttcttt ataaaaaata gtgatagtga
    23101 aaatatactt aagtgttatt taaacatagg gaaaatatat atgcaggaag acttttataa
    23161 aggtgctata agccacttta attttgcaga ggaaattttg tcagagaata aatttgagga
    23221 tgttaacata cacaaggaac tatatagtaa gatggcatat tgttacataa aactagataa
    23281 tccaaatatg tcattgaagt acatagaaaa gataaatgaa atagataata gaaatgatag
    23341 aaaagaagat gtcgatattt tggtgttaaa agcaaataat atgcttgaga ttggaaaata
    23401 tgaagaatca agagattact ttaaaaaagc actaaaaata cttgagagtg aggataataa
    23461 aactggatta gcaaatgtgt atttaacaat ttgtgatgtt tacagaaaaa taggtgaaac
    23521 agatagagta cttgaatatt cacaaaaggt atatgacata aagaaaaatg atgaagatga
    23581 atacatgatg tcaggattat ttaagataat agaagcatat atagataaag aagattatga
    23641 tttagctaga aaatattgta agatagcctt agcttcatca ataaaaaata aaaataaatt
    23701 caatgaatat aaagcattga gattctatgc taatatgtat aaaaatcaga atgaagtaac
    23761 tcttgcaatt gaatatctga ttaaatgcat aaaaatagta tcagatttag gcaacaatag
    23821 aatattggcc aatttatata tagacttagg tcaattatat tcaaatatat caaaagaaaa
    23881 agagttagaa tattatcaaa aaggtgtatt tatgtacaaa aatttagaaa taatgtaggg
    23941 taaataaacc tataagggta tagaaacaat ctataacaag caagataata tctatgataa
    24001 aatataaatt gtatgaatat ttttaaggag atgaatcaga caatgagtat aaaaatagat
    24061 gcgagagggc ttgcttgtcc taaaccagtt ataaatacaa aaagagaatt ggataaccta
    24121 gaagaaggtg ttgtagttac aatagtcgat aatgaaacag ctaaggagaa tatattaaaa
    24181 cttgctaaat ctttaagttg tgaagcaaat attgttgacg aaaaagagaa ttttatatct
    24241 atagaaatta gaaaaggtaa aaatatacta gataaacaaa atgttgtaga taataaagaa
    24301 agtgaattag acaatacatg tatatttata tcttcagata agatgggact tggaaatgac
    24361 gaattaggaa aagtgttaat aaaaggattt atatatacat taacagaatc taaaccttat
    24421 cctaagtatg tacttttagt taatggagga gtaaaattaa gtgctgaaaa tgaagaaacc
    24481 atagaaaatc taaaaatact tgagaatatg ggtgtagaag ttttatcttg tggtacttgt
    24541 ttagattatt ataatttaaa agacaaatta caagtaggta cagttacaaa tatgtatact
    24601 atagttgaga cattaaagaa tgcatcaaat accatttcta tataatgtat gtttgtttaa
    24661 taatataaaa ttaataataa agattaactc aaatagatag tcagaaagga ttaagtaatg
    24721 aatcaaatgt atatagtgtc atttaattcg acacatcatg cgataagatc agaaaagtta
    24781 tttggtgaaa atagtctaaa agtaatggct cttccaacac caagagaaat aacagcaagt
    24841 tgtggaatat caattaaatt ttcttttgaa gatatggaaa agataaaaac tattttagtt
    24901 gaaaataatg tagatataaa aggaatttat tgtataagta aattagaaaa tggctcaaaa
    24961 gaagtagaga agttagatta ggaggctaat tatttatgcc aatggattta aaaataggtg
    25021 atattgttga attaaaaaag caacatgcat gtgggtgtaa agagtttgaa atcataagaa
    25081 cgggaatgga tataaagata aaatgtacta aatgttcaag gctaataatg cttgatagag
    25141 agaccttaga aaagagagta aaaaagctaa taaaaaagtg ataaaaattt aacacttaaa
    25201 aaatgtcaaa taccgtatat taatatccaa aattttggta taataagaat tgaagaaaac
    25261 attttcatgg gggtgcttta attatggcag ttaaatatgc aaaaagaatg caaggattac
    25321 aaggatcaga aatacgtgag cttttaaaac ttactcaaca accacaaata atatcttttg
    25381 ctggtggaat gccggctcca gagttatttc cagttgagga aatgaaaaaa gtatcagttg
    25441 cagtgcttga agaaaatgga agatcagcta tgcaatatac tacaacagaa ggatatgaac
    25501 cattaagaga aaaaatagca gctagaatga atgataaaaa caaaactaat gtaaacaaag
    25561 atgatatatt agttacaagt ggttctcaac aaggtctaga ttttgcagga aaagtattta
    25621 ttgatgaggg tgatgtaatt ttatgtgaaa gtccatctta cataggagct ataaacgcat
    25681 ttaaatctta tcaacctaaa ttcatagatg ttccaacaga ttctgatgga atgataatgg
    25741 aagaattaga aaagatacta gaaacaactg atagaataaa aatgatttat gtaatacctg
    25801 acttccaaaa tccaacagga agaacttggc cacttgaaag acgtaaaaaa ttcatggaaa
    25861 tagtaaataa atttgaaata ccagtaatag aagataatcc atatggagat ttaagatttg
    25921 aaggagagac tttaccatct ttaaaatcaa tggatacaaa aggattagta atattcttag
    25981 gaactttctc taaaatattc tgcccaggat acagattagg atggacttgt gcttctccag
    26041 aaattttatc taagtttaac tttgcaaaac aaggtgcaga cttacaagca tcaactatat
    26101 ctcaaatgga agtaagcaaa tttatggata tgtatgacct agatgcacat gttgacaaaa
    26161 taaaagcagt ttatgttaaa cgtagagatg taatgcttaa gactatggaa gaagagttcc
    26221 cagaaggatt agtatttact catccagaag gtggattatt tacatgggta gagcttccaa
    26281 gtaatttaaa tgctaaagaa ttaatgccaa aatgtttaga taagaatgtt gcttatgttg
    26341 ctggaggagg attcttccct aatggtggta gagaaaatac tttcagactt aactattcaa
    26401 atatgccaga agaaaaaata atagaaggta taaaaaatat agcagctgta ttaaaagaag
    26461 ctatgggtgt agaagcttaa ttaaaaataa taacttatat ataatacaag ggatggttgt
    26521 tgccatccct tgtttgtttg aaaagtatat aattcacctt atactaaact ttgtccaaaa
    26581 tttaaattga cagaaataag aataaatgat aaaatgtgta ggtatgagat tttagtctca
    26641 tttctctgct ctaagagtgt agagccgtta agtccaaagg gaggtgaatt atagtgagaa
    26701 attatgaatt agtttatgtg gtaaagccaa actctgatga agaagtaaga gaggctatac
    26761 taaacaaagt taaggaagtt gttgcaactg acggagaaat agttaaagtt gatacttggg
    26821 gaactaaaaa attagcttat ccaatagcta agtttacaga aggtttctat gtattagtta
    26881 acttcaaatc agcagtagac gttcctaaag agatagacag aaacttaaag ataaatgaaa
    26941 acgtaataag acacatgata gttgttgctt aataactggt tttattataa aaaataggtg
    27001 gtgtttttat gaatcaagtt gtattagtgg gaaggttgac tagagaccct gaattaagat
    27061 acatcccagg tacaggaaca ccagtggcat cctttacaat agccattgat agagactatg
    27121 taaaaaaaga tggatctaaa gaaactgatt ttatacctgt tgaagtaatg ggtaaatctg
    27181 ctgaattttg tgctaactac ataactaaag gtagattagt agcacttcaa ggatctataa
    27241 gagtagacaa ttatcagaca caatcaggtg aaaagagaac ttttacaaaa gtaagtacta
    27301 gatcagtaca agctttggat agtaagaata aatcggaaaa ttcatataga gagagtgctc
    27361 cagcttttga gccaagcttt gaacctcaag gtttagaccc acaaggtttc caagctatag
    27421 atgatgacga tataccattt taaaaaggag ggaaaaaagt catgatgaat aaaaaaagac
    27481 gtaagaaaaa aagagtttgt caattttgtg ctgacaaaaa cgctaaaata gattacaaaa
    27541 gtactcaaag attacaaaag tatataactg aaagaggaaa gatattacca agaagaatat
    27601 ctggaacttg tgctaagcat caaagagaat taacagttgc tataaaaaga gcgagaaata
    27661 tagcactttt accatataca ttagactaaa aaaatgagcc ttgtgctcat tttttattat
    27721 atgtatttat atatctaaat gaggcgagct ataaagctcg tttttttgtt tatatactta
    27781 aatatattat tatttatgat ataatattat atattaagga gggttgtaaa cgtgaaaata
    27841 gataaaggtt ctgagataac gagaaacata aagataatag aatggatgaa aacagaaata
    27901 ttaatgagtg taagtgattt gtttaattta ttgtttaaag gggtaaaacc attagatgaa
    27961 gcaattcaag atactttagc aaatataata atgataacat atctcttagc aaagagatta
    28021 ggaattagtt ttagagatgt ggattataaa gtcaaggaaa aaataaaaat tggtatagat
    28081 gaggaccata gcgttgaaag atggtatgga gatttatcta atttaaaaaa acatatggat
    28141 aatagggagt gatattaaat tgaataataa aattagacta tctaaggcaa tgtctataat
    28201 agttttagct ataataatcg ctttagtaat agcctatatt ccaatgctag gaatatttag
    28261 tatgttagca gctgttccat atgtaattat aggtgctatt actgatagaa agtattctct
    28321 tatatctatt ttggttacat tttgtgtact gattctattt gcagacattt cttatgcatt
    28381 aaatatatgt ataatgtatt ctttaccagg tatagtaata ggaaagatgc taaagagaag
    28441 ctttgaacaa gaggacagta ataaattcga accgatatat ggtggaacca taatatttgt
    28501 actttcaatg cttgtatatt tttttgtatt aaagacattt ttaaatgtga atctattaga
    28561 tgaagtttct aagatgatat cagaaattgt aaatattcaa aaaaatagtt tttctgcaac
    28621 tgaacttaaa gtgtttgata aaatgaagcc agatgagatt gtaagttatt ttacaaatat
    28681 gctaccaatg atgttatttt tacaagggtt gttatcagcg tttgtcacat attatttgag
    28741 tgtatttttt ataaaaagga ttacaaagat gaatataaga tttcctaagt ttgctgattt
    28801 ttatttaccg ggaaatgcta tactcacaac tttcttactt tatttattag tcttatttat
    28861 tcaaattatt ggttcaaaat tatatacaga attaataatg actaatttac agctagtttt
    28921 taatttaatg tttgtaattc aaggtatagc agtttgcatt tatttcttaa agaaatggat
    28981 aagacaggga ccaaataaaa ttatgttttt ttcagggata attttatgtt tatttggatt
    29041 tatgggaata tcttttgttg gtatggtaga tagtataatt gactttagaa aggtaagaag
    29101 ctataaatcc acttaggagg atagaaatga gtaataaaca aacctttaaa ttaaatatgc
    29161 cagaaataaa tttatatata attgttattg gtatttctag tataattttg ctttattaca
    29221 acttgtatgt agggtgttta tttttctgta tttttgtata tatggttttt cacaattgga
    29281 gaactactaa tattagacgt catgaatgga cagagtatat tcaaaattta tctttagata
    29341 tagatgaaac aactaaaaaa gccataataa atcttccaat accattatgt attttagaat
    29401 ttgatggaaa tatatcctgg tataatggaa agttttatga tatgattggt caaaaagatt
    29461 tacttgataa aaatatagag gatatagtta aaaatcttaa tttaagaaaa gtattaaatg
    29521 aaaataaaga gatgtacaca gaaataaact ataaagaaaa agaatataca ataatttata
    29581 atgttataaa aaatgatcaa gaaaaaaatc ctaaatattt gatgatatta tactggatag
    29641 acaagactga atatttaaaa gtaaaacaaa attatgatga tgaaaaaaat gcaatgatgc
    29701 taatacaagt agatggatat gatgaagtat taaaaagtgc agcagaagat aagagagctt
    29761 taattaatgt tgaagtagag aaaatacttt ctgctttaga attaaattca aatggtgcat
    29821 tgagaagaac ttcaaaagac aaattcttct tggtaatgca taaaaaagag ttaaaaaaat
    29881 tggaagctga gaagttttct attttagata caataagaca tattgattat ggaaataatc
    29941 ttcctgttac tataagtata ggaattggta tagatggaga tacattaaat gagaacctaa
    30001 aacttgctac gggtgctctc gatttagcac ttggtagagg tggtgaccag gctgttgtaa
    30061 aaacaaaaga taaatttgtg ttttatggag gaaaatctaa agctgtagaa aagaaaacca
    30121 aagtaaaatc cagattaata ggtcatgctt taagagaggt tatacaacaa agtgaccaag
    30181 tttatataat gggacataaa tatcctgata tggatgctat gggagcagca gttggtgttt
    30241 atgatatatg taaatcctgt aacaaaactg caaacatagt tttacaatct gtaaatgaat
    30301 ctatagaaat atttataaat aaaataaatg aaaataatta ctataaaaag ttgtttattg
    30361 gaaaagagga agctattgac aactgcacta aaaatacatt agttgtagta gttgatactc
    30421 atagaccaaa ttatactgaa tgtgaagaac tgttaaagct atcagagaag gtagtagtta
    30481 tagaccatca tagaagaggt gtagagttta taaatgatgc agtgctttta ttccatgaga
    30541 tatatgtatc ctcaacgtgt gaaatggtta cagagttagt tcagtatatg gatgaagatg
    30601 taacaataaa taaacttaca gctgaagggc ttttagcagg tataagtcta gatactaaaa
    30661 actttgcatt caaaacaggt gttagaactt ttgaagctgc ttcttacctt agaaaagttg
    30721 gagcagatac tatagaggta aagaaattct tcaattctga tgtaaaagat ttcataataa
    30781 aagctgaaat aattcaaagt actaaaataa ttaataatag aatatgttta gcatattcaa
    30841 gtactgagat agatagtata aatgttataa tagctcaaac tgctgatgag ttattaaata
    30901 tcaaagaagt agaagcttcc tttgtattag gtgaaaaaga tgatactata tttataagtg
    30961 cgaggtcttt aggacaaata aatgtacatg tacttatgga aaaattagga ggcgggggac
    31021 acatagatat agctggagca cagttaaaaa atgtttctct aaaagaagct tataagatgg
    31081 taaataaaat aatagaggag tatttggagg aggaagaata gatgaaagtt atattattaa
    31141 aagacgtaaa aggaactggt aaaaagggag aaatgaaaga agtaagtgat ggatatgcaa
    31201 gaaatttctt atttcctaaa aagatggctg tgcaagcaga tagtgtagca ataaaagaat
    31261 taaatgaaaa gaataaatct aaagaaataa aagcacaaaa agagtatgaa gaagcagttt
    31321 tattaggtaa gcaaatggaa gaaattaata tagaaatata ttcaaaagca ggagaaggtg
    31381 gaagattatt tggttcaata acttctaaag aaattgctga gcaattaaag aaacaaaaag
    31441 acatagatgt agataaaaga aaaatattat tagatgaacc aataagatct ttaggttcaa
    31501 ctttcgtaga aataaaaata catcaaaaag taactactaa aataagagtg gatgtaaaag
    31561 aaaaacagta ataactagag gtgattaaga tggaagatat gacgagaatt cctcctcata
    31621 gtgtagaatc agaacaatca atattgggtt ctatacttct agataaagat gctataataa
    31681 cggttacaga aactataaag cctgatgatt tctataaaga ggctcacaaa ataatatatg
    31741 aatgtatgat aactttaagt aataaaggtg agcctataga cttgataaca ttaacagaag
    31801 aactaagaaa acaagggcat cttaatgata ttggaggaat cagttatatt acaagtcttt
    31861 ctactatagt accgacgact tcaaatgtaa aatactatgc tgatatagta aaagaaaagt
    31921 ctgtacttag aaagcttata aaagcttcta atgaaataat aaatctagga tatagtggag
    31981 caactaagat tgaagatgtt ttagaacaag ctgaaaagag tatatttgat atatcacagg
    32041 agaaaactag tgatgatttt aaatctataa acttagtttt gatggatgcc tatgatatga
    32101 tagaaaaatt atacactaac aagagtgatg tcactggtat aactactggt tttaaagatt
    32161 taaacaaaaa aataaatggt cttcaaagga ctgatttaat attaattgca gctagaccag
    32221 caatgggtaa aactgccttt tctttaaact tggttcaaaa tgcagcctta aaaggtgatg
    32281 cctctgtagc tgtatttagt ttagagatgt ctaaggaaca attagtacaa cgtatgttat
    32341 cttcacaatc tagtgtagaa ttaaaaaaaa taaagactgg aacacttaat gataatgatt
    32401 ggcctagaat tatagatgct atggcagtat tatcagatgc caaaatacac atagatgata
    32461 ctcctggtat aaaaatatca gaactaaggt caaagtgtag aaagttaaaa atagaaaaag
    32521 gactagattt agtgcttatt gattatcttc aacttatgga aggtgaaggt aataatgaaa
    32581 gcagacaaca agagatatct aagatatcaa ggtcactaaa aatattagca aaagaattaa
    32641 actgtcctgt agtagcacta tctcagttat cacgtgcacc agaacaaaga gcagaccata
    32701 gacctatgtt atctgattta agagagtctg gagcaataga acaggatgca gatatagtta
    32761 tgttcttata cagggacgaa tattatcatg ctgattctga gagtaaaaat ataggagaag
    32821 taataatagc aaaaaataga cacggtgaaa ctggttctgt ggaacttgtt tggcttggtg
    32881 aagttcaaag atttggggat aagttgagag acctataatc taacgaatat ccacagaacg
    32941 tcctttcttt ctacaggaaa aatgtggata catgtcacta aatatgtcca aacattgaaa
    33001 tttagctaaa aaactatcca cactatccac agatggcctg ttgataagtg tgaataagta
    33061 gataaagcaa gcttttggct tgctttatct gtgtataaat attaattatt aggaggaata
    33121 atatggaaaa aataagctta aaatatatat atcccaatat aattaaagta cttgatgaaa
    33181 taaacttatt tagagtaatt gacaataatt taagagaatc tattgttgtt tatgctaata
    33241 atgtggataa tcagtatcat ataaacatga ctaatacaaa ctttggaaat ataattaaca
    33301 tatgtaaatt agaaaaactt ttagatgtgg ataaatttat ggaaaaagtt ataaaatatg
    33361 aaaaagaaat aatagagaaa gaagagtttt caaaaataga agaatatatg ttaaatattg
    33421 gagaatacta aaaaagttga aaaaaatacg aatgtttgat agaataagta agtaaagtgt
    33481 tcgagagtat ataaaatata gaggtgaatt acatgaaaac agttgcaatt gttggttctc
    33541 aatggggaga tgaaggtaaa ggtaaagtta tagattatct tgctactcaa gcagatgtag
    33601 tagtaagagg gcaaggtgga aataatgcag gacatacatt agttgtagaa ggtaaaaaat
    33661 acgcattaca tctaattcca tctggagttt taaatccaaa tacagtgaat ataatcggaa
    33721 atggaatagt gtttgaccct aaaggatttt tagaagaatt ggaaatgttt aaaacggata
    33781 atataagtac agaaaatata aaaataagtg atagagcaca tgtcatattc ccatatcata
    33841 aagaactaga tgcattgtca gaagaagcca gaggcgattt aaagataggt acaacaaaaa
    33901 aaggtattgg accttgttat atggataaaa cagagagatc tggtattaga atatgtgact
    33961 taatggataa agataaattt gctataaaat taaaagctca aatagatgct aaaaatgaaa
    34021 tagttaaaaa tatatatgga aaagaagaat tatttgattt tgaaactata tataacgaat
    34081 atctaggata tgcagagcaa ataagaaaat atgtggcaga tacgtctgtt attgtatatg
    34141 atgcagtaag agcaggtaaa aaagtattgt ttgaaggagc acaagggact ttattagatt
    34201 tagatttagg aacttatcca tttgttacat cttctcatcc aacatctggt ggatttgcaa
    34261 ttggtgcagg tatagggcca aatatgataa aagacgttgt tggtatagta aaagcatata
    34321 ctacaagagt tggagaagga ccgtttgtta cagagcaaat aaatgaaact ggagataaga
    34381 taagagaaca aggacatgaa tttggggtaa ctactggtag accaagaaga tgtgggtggt
    34441 ttgatgcagt tatagttaag tatgcagcta gagttaatgg attgactagt atttcattta
    34501 tgttacttga tgtattaact ggatttgata agataaaagt ttgtacgtct tataaaatgg
    34561 gcgataagat aataacggat ttcccagctt cattagatga tttagcaaaa tgtgaacctg
    34621 tatatgaaga attagatgga tggaatgaag atataactca aatagataac tttgatgacc
    34681 ttccagaaaa tgcaaagaaa tatgtagcaa aaatagaaga actagttgga gtaagtgtag
    34741 atatggtttc tgttggtcca aatagagctc aaactataat aagaagaaat atatttgctt
    34801 aatattttat caattaaata atttaaaata gttgaagaag agtatctcaa gataatgttt
    34861 atagttatta gagatgctct tttatatttg acttacattg attttttatt aagttaaagc
    34921 tacttataca tattctaact gaaattaaag ttgaatttat gtagatacat ataggttttt
    34981 taaaaagaat tttgttgaaa ataaatggct aaagatgatt ttaattatat tctaaaaaaa
    35041 gtaaaaaaaa ctattgacta aaaattatat agacgctata atataacttg tgagtgaaag
    35101 aaacgacgaa aaaagtcgaa aaaatgtggt ctattagctc agtcggtaga gcacctgact
    35161 tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca ccaaaatatg gctcgttggt
    35221 caagaggtta agacaccgcc ctttcacggc ggtaacaggg gttcaattcc cctacgagtc
    35281 accagtatgg gactatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag
    35341 gttcgagccc tgttagtccc accaaatttt gaattagaga taaatactag attatatgct
    35401 ggtgtggctc aacggtagag cagctgactt gtaatcagca ggttgtaggt tcgattccta
    35461 tcaccagctc cacaaagtac ataaattcaa attaatataa ttttctatgt tacaggatac
    35521 ttttaagtat ctttttttaa tttcattttt attgtattaa aaatgaaatt aaacatacaa
    35581 caaaattttg aattacttgc aatgtgatag ttttaaatat aaaatttaga atcattctag
    35641 cataatatta tgtaatatag caaatttaat ttatagtatc aataaatata gactactaag
    35701 aatgtaaatg atttgttaga ttaaggtaca ttagatatgt aaatagaaaa ataaaaagta
    35761 tgttttaatt tatgtgatat aagttttaaa cagagaagca agtttgctct tacctctcta
    35821 tttaaaaata aaaaaatgtt ttttaagggc tattttattt aaatctttcc catctcaaat
    35881 catctccagt aaattctaaa atcctaaact tatctaatat tctagagaga gttctttgag
    35941 tgtaagtatt tcctatttga gatggtgtta agttggttga aataatgatt tttttaccag
    36001 caacaagtct tgtatttaca atattgaaga tttctccact agtaaaagaa ttattaagtt
    36061 cagttccaag gtcatctatt attaataaat cacaatcaaa caagttttta taattatctt
    36121 cacttatttg attatttgtg tctcttctaa atttataatc ttcaagaata tctaaaattc
    36181 taaaagaagt ttgatagatt acaacattgc ctttatctaa caattccttt gcaatacaat
    36241 tgcacatata ggtctttcct agtccagtag aaccataaaa cagtagattt tcactattat
    36301 ccttcttaaa atcatgaaca aaattttcgc agatacttaa attatttagc atattttctc
    36361 taggagatat ttcaccatca gagcctttct taggagaaaa tatattcaag ttaaaattcg
    36421 aaaaattttc ctgacttaaa atcctatcta aattagacat tttataagct tcatttacaa
    36481 tttcttgttt gaggcaagaa catttttctc cattaggtaa aaatccttta tctttacaag
    36541 aaatacattg aaactttatt tccaagtaat caagaggtat gttactttca gctaaaagtc
    36601 tttctttctt tacttttaaa gattctatat tctctttagt cttctttaca atttcatctt
    36661 tagacttagg atttaaaagt actatcttag ctaaactgag acctatttta gaaatttcat
    36721 catctataga ttttatctca gggattctat tatatacctc attttttcta tgttctaata
    36781 agagttcatt atcatctctt ctcttagcat atttagcaag tatttttctt attttatcct
    36841 cattcattta atcacctact taaacttttc tttttgactt tttttaataa tctcatcgag
    36901 ctcatcagag gtatactgag tgaatgtttc gttgaaatta tgaaacttag tttttttgta
    36961 agtattgttg ttttgagtag tattaatctg cttcttatta atattctcct taactatacg
    37021 ttcttcctct ttttgcttta aatcatttaa attttttata tttttttcat tccaattttt
    37081 aattatacca tttatataag atatacttgg gtttgaaata ttcttagact tagaacatgc
    37141 atttattatc aaatctatat ccatattaaa tttatcaaac caggtgtcca taagttcttt
    37201 ttctgatttt gatggttgtc ttgaaaatcc caattcattg aaaatcgttt tatataaaat
    37261 atatcgttca cttcttacca aaaagctttc ttcaacatct tttggcgtat ataaatttga
    37321 atcataccaa tttctaataa tgccttcaat gtatttaact ggctttgatg taccagtttt
    37381 atctttgacg tattcatatg cgcaaagtat catatcgggg ctcatattgt atttattcat
    37441 tatatccata atactcatct tttcacttgg gtcgagatat cgcccaacta ttttatttat
    37501 agaattaaac atttttctaa tacttggatt ttcagaagta gatacaactt tatctgtatt
    37561 agattttata gatgaattat tgcttaatat attctcaata tagaaatttt ttaaatctaa
    37621 aaattctatt gaataattaa aattatcata ttcttcattg tcatgtattt ttataatctt
    37681 cttttcttcc cagaatttcc aagcagataa cacatcagac aatggtatat ttaggttttt
    37741 ggctattgaa ttattgtcga acttaggatt agaagtaata tcacatgctt gtctatatcc
    37801 taaaagataa acttttacgt ataacccgtc cgccattggc ataaatatat ctataaagat
    37861 attgggaata gtgatttcac ctaaatctat ttcattgctt tctttaaaaa acattttatc
    37921 acctcagatt aaattatatc attttttata ttatatttgt ctgtaaacaa ataaaaggtc
    37981 acaagattag catgtaaata ttttctaaaa gtattcattt tgaaaaggag gagatatttt
    38041 ggttataaaa ttcaaccaaa aagtaagaag aacattatca gtagcgattc tatttatggt
    38101 attaataata ggttgcttta tagaaaagga tgacagttta gaaacataca aagaaaaaac
    38161 taattcaaaa ataaatcagt ataatatgga tgttatattt gatgatgaga ctaagaggtt
    38221 aatgtgcaat cagaatgttg actacataaa taatacaaaa tcaaacatag ataaaatata
    38281 tttccatata tatccgaatg ctttttccaa gaaagatttt gctccttttg aaaaaagtga
    38341 aatgacaagg gcttatccta atggatttaa cgaaggatat atagatataa agaatatatt
    38401 aaataataat agtaaaatgg agtataaaat taaaggagac aaaaatgaca tattagaaat
    38461 agaattaggg aaagagttaa aaccaaatga aagaatgtca ttagatataa aatataacgt
    38521 aaaaatacca aattcaattg gaagatttgg atatggagaa aatactataa atgttactaa
    38581 ttggtttcct atagcttgtg taaatgatga aaggggatgg aatttaaaga gttatgaaac
    38641 tattggcgac ccattttata gtgagactag cgattttcat gtgaaactat tgattccaaa
    38701 taaatataaa attgctcata caggaaaact tattgatgat aaacatgata ataagaaaat
    38761 gctatatgaa atagaagctg aaaacgttag agattttgca tttatactaa gtagtaaatt
    38821 tgatgtaaac aaagtagatt caaagggagt agctatcaat acatataatt taaataaaaa
    38881 gctttctaaa aaagcaactg aagtggcaaa agattcaata aatatattta gtgaattatt
    38941 tggaaagtat ccttacaaag aattttctgt tgtagcaagt gactttttta taggggggat
    39001 ggaataccct atgcttgtta tgatagacca aagtttatat aatgaaaaaa atgaattttt
    39061 actagaatat gttattgccc atgagacagc acatcaatgg tggtattctg ctgttggaaa
    39121 tgatgaaata agtgaaccct ggttagatga agcacttact gaatattcta caatagttta
    39181 ttttgaacaa aagtatggta aagatatggc aaacaagctt ataaaaacta tggagataca
    39241 gacaaaaagt tatttgagtg aaaatatatt taagccagca aatgagtata aaaattctac
    39301 tgaatacagc ttgaatgttt atacaaaagg cgcaatagca tttaatgaaa taagaaaaga
    39361 agttggagat aaagtattct ttgagacttt aaatgagtac tataataaat ataagcataa
    39421 aaatgctaat ggaagagctt ttgtggaact atggaatagt aaaggtgtag atataaataa
    39481 gattataagt gaatgtaagt aaatagtgaa tgtaagtaaa ttttgtgcag agtattaaat
    39541 tttatttaat actctgtttt tttggtataa tataatttat aaggcagaaa aagaggtgaa
    39601 ggaatgctaa agtattgttc agtagggagt ggtagtagtg gtaattgcca ctatataggg
    39661 tataaaaata ctaatatact agtagatgca ggattaagtg gcaagagaat aactacaggt
    39721 cttaaagaca ttggtgtaga tgcagataaa ttaaaaggaa tatttataac acatgagcat
    39781 gttgaccata taaaaggagc aggtatatta tcaagaaagt ttgatatacc agtatttgct
    39841 aatataaaaa catggtgttc tatgaaggac aaattaggtg acatcaaaga taaaaatatg
    39901 aaagtatttg aaaatgataa aacctactca ttaggtgata tcatagtaag acctttttca
    39961 atagaacatg atgcgtctga cccagttgga tataattttt atacagagag tgatgaaaaa
    40021 atgtctatag ctactgatat aggatgtata actcaaaata taaaaaaaca tctatataaa
    40081 tctaaattag tagtattgga atcaaattat gaccctaata tgcttatgat gggttcatat
    40141 acatatgctt taaagaaaag agtaatgtca agtactggtc atttatccaa tgaagatgca
    40201 gctaatttct gcgttgaact tattaatgaa ggtacagaat ctatattatt agcacattta
    40261 agcaaagaaa ataattttcc tgagttagcg tatgagactt caaaaggagt attagcatca
    40321 aatgatatag tagttggaca agatgtaaaa ctagatgtac tatcacgaaa tgatgtttct
    40381 aatgtttatg agatgaagta gaggtgaata actttgctaa agagaaattt tgaaaatgtt
    40441 gaggataata aaattttata ctctataaag cagggtatga ttttagcgat acctgctatt
    40501 atgactggtt caactgctct tgtaattttg aatcttccaa taaaggctta tcaagaatat
    40561 ttatcaggct tgtttaatgg agaggttaca aatattctta actttataaa tgattctact
    40621 ttaggaatta tttcattaat aatattatta acaataagct atagttatgg aaaaatatat
    40681 gggagcaagt atacagtact tgttcctata gttgcaatgt gctctttttt agtattttca
    40741 catggtaatg agcttaattc ttatatagaa atatttaaaa caaagtggtt atttacatct
    40801 ataattgttt ctatgacatc ttctgtattg tttgtaaaac ttacagaatc ttttgttaca
    40861 agtgtaaagt ttcatacaga aggggctgat gcagacttta atatggtagt ttcggctata
    40921 gttcctttca taatagtagt atttttgttt tctattttta gagtggtaat gataagtcta
    40981 attggcagtt caaactttca agatatattt tataatttat tttcaaatgt ttttaataaa
    41041 atgggaacaa accttatgag tgctctgttg tttatttttt taatgcactt tatgtggttt
    41101 tttgggattc atggtagtaa tgtattagat accgtcgcaa aaaatttatt tgaaaatagt
    41161 atggcaatta acataaattt agttaataat aatcaattac caacagaaat ttttactaag
    41221 accttttttg atacaatggt tttattggga ggatgtggct ctttactatg tttagtaatt
    41281 gcaatttttc ttagtgaaaa acgtataaat gtaagaaagt tagctaaaat tgcttcaata
    41341 ccagctcttt ttaatatcaa tgagatgcta gttttcggga taccaattgt ttttaactat
    41401 attatgtttg taccttttgt aattactcct ataatcttaa ccgttacaag ttatgtggct
    41461 atgtctacag gtattgtacc atgtacagtt tcagctgtag aatggacctc acctatattt
    41521 ttaagtggat atatgtctac aggttcaata agaggtagtt tgttgcaagt atttaattta
    41581 tgtgttggtg ttatgattta tattccgttt ataaaaatgt cgcaaaatag atatacatat
    41641 atgtttaaaa ataatataga aaatttaaca aacttattaa aaaggtatga attaacaggt
    41701 gaacagccta atcttctaaa ccataatggt aaaataggtt caatttcaaa aatgttggca
    41761 tcagacttga agtttgctat gaaaagagga gaaatagagc ttttttatca accacaagtc
    41821 aattataatg gaaatgttgt gggagcagag ggattactta gatggaaaca ttcaatagga
    41881 gggttcattt atccaccact agttataatt ttggctaaag aagaagggtt cttagatgag
    41941 cttggagagt atattattaa taaagcatgt attgatttaa agaagagtga aaagctattg
    42001 agaaatcctg ttaagttctc tgtaaatata tctccagacc aacttgatga ccctaagtta
    42061 ccagagcaaa taaaaaatat cattagtagt aatgatataa atcctaatat gttaggtata
    42121 gaaataacag agcaaattgc attatcaggt tcacaaataa ttattgaacg tatcaatgcg
    42181 atacataact tgggaataaa gttaattatg gatgattttg ggatggggca tagctctctt
    42241 atatatcttc aaaataataa ctttgatata gttaaattgg atggttcatt agttaaagag
    42301 atacttacaa ataaaagaag tagtgaaatt ataatgacta ttgtaaatct ttcaaaaaat
    42361 ttagattttg atatagttgt tgagtatgtt gaaaatatag accaaaggga taagttatat
    42421 ggtttaggat gtgaaatata tcaaggatat tattatagca aagcaatacc ttttgaagag
    42481 tttgtagaat atgcaaatca agaaagagag atataatttt ataagagctt gtaaattcta
    42541 gtaatgcata gatttgtaat aaaattaagt ttaggagaaa tatatgaata taagtatagt
    42601 agtagtaggt aaaattaaag aaaaatattt aaaattaggt attgatgagt ttaagaaaag
    42661 gctttctaag tattgtaagt tagaaattat agagttggat gatgaaaaag ctccagagaa
    42721 tttgagtgtt aaggaaatgg aaattataaa agacaaagaa ggaaagaaaa ttttagggaa
    42781 gataaagcat aatagttatg ttatagcctt ggctattgat ggtaagggtt tatcctctga
    42841 agagcttgct aagactatga gtgatttagc agttagaggt aatagtagtc tctgttttat
    42901 aataggaggt tctttagggc tttctgatga ggttttagga agggctgatt ataggttatc
    42961 cttttctaag atgacttttc ctcatcagat gatgagattg atattgttag aacagattta
    43021 tagggcttat aggataaata atggagaacc gtatcataaa taaatgatac ggtttttgtt
    43081 aatttgacac ttaataatat taaaataaca aaaaagtatt gaaagtatat tgcatttaag
    43141 gtagaattaa ataaaatatt ataacattat aatgtttata tggttgttaa taaaaatttg
    43201 gaggaaaata tatgataaaa ataatgttgg cctgttcagc aggaatgtct acaagtttat
    43261 tagttactaa aatggaaagt gcagctaaag aaaatggtat agagtcgcaa atttgggcaa
    43321 taccagaatc aactatacaa aatgaaatag aaaaatgtga tgtattatta ctaggaccac
    43381 aagttagata tgtattacct aaggctcaag aaattgctaa gccatataat ataccagtag
    43441 aagttataaa tatgatgcac tatggaacag ttaatggaga agctgtttta aatagagcga
    43501 tagaacttaa caacactaag taatataaat tataatgtat ttttaatata taaattggga
    43561 gaatcaaagg atggatgaaa agactataga aatatctttt aatataatag gttatgcagg
    43621 tgaaggtaaa ggattagctt ttgaagcaat aaaagaggct aagaatggaa atatagaaaa
    43681 agcaaaagaa ttattaaaag agtctaggga agttataaat aaagcacata gatatcaaac
    43741 agaattaata caaaacgaag ctagtggaaa caggactgaa ataagtgtca tacttataca
    43801 tgcacaggac cacttaatga atggcatgaa ttttcaacag ttagctgagg aaatcgttga
    43861 tttacatttg aaattacaag ataaataaaa attaaagata ataaattaag agagttctta
    43921 aaagatacat tatgtgtagc acactaattg ttagattatt gattcaatat ttagtgagta
    43981 atacattagg attattttga ggactctttt tatatttagt ttaaagttaa taaatattta
    44041 ttattaataa tataa
//
LOCUS       DAMJZE010000015        43943 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJZE010000015 DAMJZE010000000
VERSION     DAMJZE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.015"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            143..493
                     /locus_tag="PC541_000729"
     CDS             143..493
                     /locus_tag="PC541_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2838203.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            512..2155
                     /locus_tag="PC541_000730"
     CDS             512..2155
                     /locus_tag="PC541_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2838204.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2236..4293
                     /gene="recG"
                     /locus_tag="PC541_000731"
     CDS             2236..4293
                     /gene="recG"
                     /locus_tag="PC541_000731"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2838205.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4352..4906
                     /gene="rsmD"
                     /locus_tag="PC541_000732"
     CDS             4352..4906
                     /gene="rsmD"
                     /locus_tag="PC541_000732"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2838206.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4908..5405
                     /gene="coaD"
                     /locus_tag="PC541_000733"
     CDS             4908..5405
                     /gene="coaD"
                     /locus_tag="PC541_000733"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2838207.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5409..5933
                     /locus_tag="PC541_000734"
     CDS             5409..5933
                     /locus_tag="PC541_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838208.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6277..8196
                     /locus_tag="PC541_000735"
     CDS             6277..8196
                     /locus_tag="PC541_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2838209.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8209..8496
                     /locus_tag="PC541_000736"
     CDS             8209..8496
                     /locus_tag="PC541_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838210.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8629..9888
                     /locus_tag="PC541_000737"
     CDS             8629..9888
                     /locus_tag="PC541_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2838211.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9891..10631
                     /locus_tag="PC541_000738"
     CDS             9891..10631
                     /locus_tag="PC541_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2838212.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10954..12264
                     /locus_tag="PC541_000739"
     CDS             10954..12264
                     /locus_tag="PC541_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2838213.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12362..13279
                     /locus_tag="PC541_000740"
     CDS             12362..13279
                     /locus_tag="PC541_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2838214.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13295..14104
                     /locus_tag="PC541_000741"
     CDS             13295..14104
                     /locus_tag="PC541_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2838215.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14162..15910
                     /locus_tag="PC541_000742"
     CDS             14162..15910
                     /locus_tag="PC541_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2838216.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16172..17194
                     /locus_tag="PC541_000743"
     CDS             16172..17194
                     /locus_tag="PC541_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2838217.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEVSRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17199..19505
                     /locus_tag="PC541_000744"
     CDS             17199..19505
                     /locus_tag="PC541_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2838218.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19477..20592
                     /locus_tag="PC541_000745"
     CDS             19477..20592
                     /locus_tag="PC541_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838219.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20610..22028
                     /locus_tag="PC541_000746"
     CDS             20610..22028
                     /locus_tag="PC541_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2838220.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22043..23251
                     /locus_tag="PC541_000747"
     CDS             22043..23251
                     /locus_tag="PC541_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838221.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23325..24713)
                     /locus_tag="PC541_000748"
     CDS             complement(23325..24713)
                     /locus_tag="PC541_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2838222.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25054..26388
                     /locus_tag="PC541_000749"
     CDS             25054..26388
                     /locus_tag="PC541_000749"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2838223.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26530..27447)
                     /locus_tag="PC541_000750"
     CDS             complement(26530..27447)
                     /locus_tag="PC541_000750"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2838224.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27655..28539
                     /locus_tag="PC541_000751"
     CDS             27655..28539
                     /locus_tag="PC541_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2838225.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28752..30593
                     /locus_tag="PC541_000752"
     CDS             28752..30593
                     /locus_tag="PC541_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF2838226.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30898..31605
                     /locus_tag="PC541_000753"
     CDS             30898..31605
                     /locus_tag="PC541_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838227.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31599..32909
                     /locus_tag="PC541_000754"
     CDS             31599..32909
                     /locus_tag="PC541_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838228.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33026..33709
                     /locus_tag="PC541_000755"
     CDS             33026..33709
                     /locus_tag="PC541_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838229.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33702..36089
                     /locus_tag="PC541_000756"
     CDS             33702..36089
                     /locus_tag="PC541_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2838230.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36369..37511
                     /locus_tag="PC541_000757"
     CDS             36369..37511
                     /locus_tag="PC541_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2838231.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37530..38054
                     /locus_tag="PC541_000758"
     CDS             37530..38054
                     /locus_tag="PC541_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2838232.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38154..38972
                     /locus_tag="PC541_000759"
     CDS             38154..38972
                     /locus_tag="PC541_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2838233.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39050..39361
                     /locus_tag="PC541_000760"
     CDS             39050..39361
                     /locus_tag="PC541_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2838234.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39538..40221)
                     /locus_tag="PC541_000761"
     CDS             complement(39538..40221)
                     /locus_tag="PC541_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838235.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40314..40955)
                     /locus_tag="PC541_000762"
     CDS             complement(40314..40955)
                     /locus_tag="PC541_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2838236.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41129..43132)
                     /locus_tag="PC541_000763"
     CDS             complement(41129..43132)
                     /locus_tag="PC541_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2838237.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43285..43923)
                     /gene="recX"
                     /locus_tag="PC541_000764"
     CDS             complement(43285..43923)
                     /gene="recX"
                     /locus_tag="PC541_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2838238.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        17099 a         4807 c         7551 g        14486 t
ORIGIN      
        1 tttatccaaa aacttttcta ggtttaaatt ataccatatt tatagtatga tatgattata
       61 attttatata ataaataagt aaaaatagta aaaaataaat taagaaatgt ttttaataaa
      121 aataagctta agggggtcaa ccatgagtgc aaaggtaacg aatcaatatg gaaatataga
      181 aatagataat caagtaatag ctcaagtcgt atatagagct gcaatggaaa gttatggctt
      241 agtaggttta gcatataagt ctaaaggaat agtagagcta ttaaaaggcg agaacgcaac
      301 taaaggtgta agagttgagg aagtagaaga agatgccata gcaatcgaac tttatgttat
      361 aatacaatat ggtacaaata tatctactgt tgccaataat ataatagata gagtaaaata
      421 tgtagttgaa aaaatgactg gcgtaagagt tactagaatt gatattaatg tacaaggaat
      481 aagagttaag taatttcata ggaggaaaca aatgattcaa tatatagatg gtaaaaggtt
      541 aagggagatg tttatttcag gtgcaaacaa tctccaaaac aataaagagt tagttgacaa
      601 gttaaatgta ttccctgtac cagatggaga tacaggaact aatatgtcat taacaatatc
      661 ttatgcctta aaagaattag caaaagttga aaatgataat atatcagata tcggaaaagc
      721 attatctaaa ggttctttaa tgggagctag aggaaactca ggagttatac tttcacaaat
      781 aatcagaggt atagctaaat ctatagaagg aaaaagtaaa ttatcaacag aagatttagc
      841 aaaagctttt aaaaatggtt cagacacagc atataaagct gtaataaaac caattgaagg
      901 aactatactt actgtagtta gagaaagtgg agagtttgca ataaaaactg cgaaaaaaga
      961 gaaagatgta gtaaagtttt taagtatgct tgtgaaggaa tcaaattctt cattagaaag
     1021 aactccagac cttctaaaga atctaaaaga agctggtgtg gttgactctg gtggaaaggg
     1081 tcttgtactt atatatgaag gaatgttagc ttctataaaa ggcgataaca tagaaataaa
     1141 aaatgttgac ctagatacta atatttcaac aagtatggat tttgcaaaga gtactactag
     1201 cacagatgat ataaaatatt gctattgtac agaatttatt ttagaaagtt ctaaggtaga
     1261 agatactaaa ataagagaca taatgatggc ttatggtgat agcctagcag tagttggaga
     1321 tgatggagtc ataaaggtac atgtacacac taatgaccca ggaaatgtac ttcaagaagc
     1381 tctaaagtac ggtcaattat taactataaa aatagaaaat atgaagttac aacatgaaaa
     1441 tacattactg gatgtagaag aaaagaaaga aaatgatagt gaacctttag aagaagaaaa
     1501 agagtttggg tttatagcta cttctatggg tgaaggtttg gcaaatatat ttaaagattt
     1561 cggagttgac catataatag aaggtggtca aactatgaat ccaagtactg aagattttat
     1621 gaatgctata aagcatataa atgctaaaaa tatatttatt ttcccaaata acagcaatat
     1681 aataatggct gctaatcagg caaaagaatt gagtgataaa aatataatag ttatacctac
     1741 aaaaaatacg cctcaaggat ttgctgcatt agtaactttt aatggagaat taagcgaaga
     1801 tgaaaataaa gaagctatga tgaatgcttt aaactcagtt aaatcaggtc aagtaacttt
     1861 tgctgttagg gatacagtga tgaatgaaat tgatgttaaa gaaggtaata taattggaat
     1921 tgcagaagga aatttattat cagcaggaga ttatgtggat gaagtaacat caaaccttat
     1981 tgaaaagttg gttgatgaag atactgctat aataacatta ttctttggtg aagatgttac
     2041 agaatctcaa gcaaatgaac tacgtacttc tttagaagaa aagtttgaag atgtagatgt
     2101 agagttatat tatggaggac aacctcttta ttattactta atttcagttg aataatgatt
     2161 ataagaacct gtatgaccaa gtagctttta taaaattggt tgtacaggat ttttgtgtag
     2221 taggtgatat atttgttgga tttatataaa gatgttcaat atgttaaggg aataggacct
     2281 aaaaaagcag ataaattaaa taaactgggt atatttacac taaaagacct attgtattat
     2341 tttccaagac aatttgaaga tagaaataat ctaaagaaaa tagcacaatt agaagatggt
     2401 gagaaagtta cgataaaagc agtaatatca agtataaata catttagccc aaaagaaggt
     2461 atgactttaa caaagattga tgtaaaagat gagacaggct cggctaaatt agtgtttttt
     2521 aataagagtt atataaaaaa tacttttcgt ccaggagatt caatattagt ttttgggaag
     2581 gttaagaaaa aatttaataa cttagaactt acatcatgtg aattagaata tcttactaat
     2641 tcacctaaga atacatgtag atttatgcct gtgtaccagc ttacatacgg tgttacaaat
     2701 aaagaaatta tgagtataat tagaacagta cttgaagata aagaattaat tatacaagaa
     2761 tatatgccac aaagaattat agaaaaatat aggctatgta gtatagattt tgcagttaga
     2821 aacatacatt caccaagtag taaggaatca ttaaaaatag ctttatatag aattgtattt
     2881 gaagaattac ttatactaca actagggttg tttgtattta aaagtggcag aaataaagaa
     2941 gatggaataa agtttgaaac aagtaaagac ttaaaaaaaa taataagtgc tttaccattt
     3001 aaactgacta aagctcaaaa cagggcatta gatgaaatta tacaagatat gaatttagag
     3061 aaaataatga atagattggt acaaggtgat gtagggagtg gtaaaacagt agtagctctc
     3121 ttagcacttg caaattgtgt tttaaatggt tatcagggcg ctttgatggc acctacagag
     3181 atattagcag ggcaacatta tatttcactt actgagtctc taaaagattt tggaataaat
     3241 gtaggtctat taataggtag tcttacaaaa aaacaaaagg atacagtatt ggaacaaata
     3301 aagaataatg agattgatat attaataggt acacatgcac taattgaaga taaggttgaa
     3361 tttaataata ttggtttagt tataacagat gaacaacata gatttggtgt tatgcaaagg
     3421 agcaaacttt cattgaaagg agcaaatcct gatatattag taatgacagc aacaccaata
     3481 ccaagaactt tagcacttat actctatgga gatttagata tttctattat agatgaactt
     3541 cctccaggaa gacagccaat agaaacaatt gctattgaaa aaagtaagcg agatagagca
     3601 tataataatc ttgtaagaag agaggttgaa tcaggaagac aagtgtacat tgtatgtccc
     3661 ttggtagaag aaagtgaagc catagaagcc aaatcagcag ttgaacttgt tgaggagtta
     3721 agagcggaat attttcacga cttaaggctt gggttgcttc atggtaaaat gaagtcaagt
     3781 gaaaaagatg aagtaatgag actttttaaa gataaagaaa tagatatttt agtttcaaca
     3841 acagtaatag aagttggtgt caatgtgcca aatgcaacat taatgataat agaaaatgca
     3901 gaaagatttg gacttgcaca attacatcag cttagagggc gagttggaag aggtagtcat
     3961 aaatcgtatt gtgtactaat ttatgattct aaaacagatg tatgtagaca aagaatggct
     4021 ataatggaag agaccaatga tggttttaag atttcagaga aggatttaga aataagagga
     4081 cctggggaat tttttgggac aaggcagcat ggtcttccag aacttaaggt agcaaactta
     4141 tttaagcata taaaaatact gaagttagca cagcaagaag ctcgatatat attgggtgaa
     4201 gataataatc tacaattaaa agaaaacatg gcactcaaaa aagaaattat agataaattt
     4261 aaagatacat tgaaagaaat ttcattaaat taattttaaa ttgtgttaaa atattactaa
     4321 tgtaataatg tgctgaaggg agattgtcat attgagagta atttcaggaa aagcgagagg
     4381 attaaaacta aatactccaa aaaatgaaga tgtaaggcca actacagata gagtaaaaga
     4441 gtctctattt aatatgataa attcatatat aatggaaagt gaagttctgg atttatttgc
     4501 tgggactggt tctttaggaa tagagtgctt atctaggggt gccaagtcat gtacttttgt
     4561 agatattagt aaggaaagca tagatattgt taagtccaat ataaaaaagg caagagttga
     4621 aagtgaaagt attattttaa accttgattt taagactgca atagataaac ttaaattaca
     4681 aaatagtaaa tttgacataa tttttatgga ccctccgtat tataaaaata tgtttataga
     4741 agctattgaa aaaattgata attctaatct attgaacgaa gatggaataa tagttgtaga
     4801 acatgatacg aatgatttat ttccagataa gatatgtaaa ttggagaaaa ctagagataa
     4861 gaaatatgga aatactacat taacttttta taagatggag gcataatatg gaaaataaac
     4921 ctagaaaagc tatatttgca ggaagttttg acccaattac taatggacat ttagatatta
     4981 tttgcagggc ttcgaagtta tttgacgaat tacaaatagg agtattaaat aatcctaata
     5041 aaaagggtct atttagcttt gatgaaagag taaaacttat agaaaaaagt acaagccatt
     5101 taaataatat aaaagttgtg acttttgatg gtttattaat aaattactgt caggaaaatg
     5161 ggattggagc attagttaga ggtgtgagaa gtggtgctga tgttgattat gaacttcaga
     5221 tggcacatat gaatagagag cttaatccag atatagagac tataatttta ccaagttgta
     5281 caaaatattc atttataagt tcatctttaa taaaagaggt tttgctcttt gatgctgata
     5341 taaaaaattt ggttccaaag attgttttgg aagaattaaa gaaaaaaact agtgggggta
     5401 attgatatat gaagatagat ttagaaatga ttgaactatt tgagcagtta gaggagatat
     5461 ttagaagtgc ttctacaatc cctttctcac ataaatgcac agtggataaa gatgaggttt
     5521 tagcaataat taatgatata agaactttaa taccagaaga agtaacacag gcagtatgga
     5581 ttaataaaga aagaaataaa ataataagtc aagctaagca agatgctaca aatatagttg
     5641 aacaagcgaa aaaagaagct gagagaatac aacaagaata tcaagataat atagaggaat
     5701 taaagaaaaa ttctgaagat gtagtaaagg catatgtaga atccagtgaa cctgttgttc
     5761 aagcagacca aagagcaaaa gatatagttg atagagctga gagaatagcc aatgagataa
     5821 gaattgggtc aattgaatat gcagaggatg tgctttctag tgttgagtat aatttaagag
     5881 aaatattaga agaagtaaaa agagataaag ctgaacttag acctaaaaaa taaattttaa
     5941 ataaataaaa atggagctat cacacaaaaa attccaattt atgtgtgata gctctctttt
     6001 aatatagcaa ttttttgtta tataattgtg ctttttagat tacttatatc ttattttgat
     6061 attataatgt agtttaatct tttatttgtt gaaatcaact tatttatttg tataaataat
     6121 ataggttatt ttcaaatttt tttaataatt ttaccactgt cttcttttag attagagtta
     6181 gactgtgcaa aattataaaa taatactaaa tcatgtgcaa ttatttggta tggcactaca
     6241 tgtagaaaat atagataata aaattaagga ggtagtatga aggaaagggc taaaaaaata
     6301 ttagatttta ttattaaaaa taacaatgta acatcacaag aactacaaga aaagtttaat
     6361 gtttcaaaga gaactattta ttacgatatt ctagctataa atgaacaact aggaaaaagt
     6421 ggtaatataa aaaatgtaaa acacaaattt atatttgaag gtaatttatg tgatgcaagg
     6481 aaaataattt caactgaaga agataaattt ttagatagtg attacagaaa aacttttata
     6541 ttaaacaaga tattacttgg cgaaaaaata tcaatagaaa aactaacaaa tgaaatgtta
     6601 ctttcaaaaa acacagtagt acaaacaata actgatgtaa aaaaatacct tcaaacaatg
     6661 gggttaagat tagagtataa gggaaagtac aaaattatag gtgatgaata cgttattaga
     6721 gagctgtttc tgataattgt tcaagaaaat atattggaaa ttaattctat aagtgaggaa
     6781 gtaagtagtt tcgatacaaa aggacatata aaacttactg attattcttt attaaatcta
     6841 actaagtttg tggaatttct aaataaaagg ataagagatg ggaaaacatt atactcatat
     6901 aaatatctaa atgaagctaa aaaaattagt tactttagta attgtaaaga acttttatgt
     6961 gaagaagcta atgaaaatga acaagcctat atatgcactt acataagttc acttcccagc
     7021 ttaaatagtg aagttaagga agatgttgtt gaagaatatg tagataaact tatagataag
     7081 tttgaagtaa atacagcaat aaaattagaa agtaagcatg agtttaagaa aaatatttta
     7141 aggcacctcc attcttctta caacagaata cgatttaagt ttcctattag aaatccaatg
     7201 ttagatgaga ctaagtataa acatgagtca ctttataaaa ttataaaatc aattatagaa
     7261 aatgaagaag agtttcctgt atttgagggg ataagagaag aagaaatagg atttatagca
     7321 gcgtattttg gaggatattt aagagggtct agggataatg gtctaagaag aaataaagtc
     7381 ttactagttt gtcctaatgg tcttatggta tcaaagagtt tggaaataca actatataaa
     7441 tacataccaa ccatagaaat agtaggtata gtatccatta agcaactaaa agaagtaaat
     7501 gtatactacg attacataat tacaactata gatatacaaa atgtaaacaa cgtaattgtt
     7561 gtaaatcctc ttttaacatc atcagatgtt caattactta tgaacaaact aataagtgta
     7621 aaagaaaacg agaagtattt caatcttgaa ttaataatac aagctataag aaaaaatgga
     7681 gttatcaata atgaagaagc actaaaagct gatttattaa acataataca caagatagat
     7741 gaaggagaga tgtatcaacc aatgttaaag gaattaatta atgcagaaag ggtcaatata
     7801 ataaaaagtg ttagagattg gaaagaagca ataaaaatag cctcaaagcc attgttagaa
     7861 gataactcta tagaagagtt atatatagaa aatatgataa agtcagttga gaaatatgga
     7921 ccatatattg ttttagctga tagatttgct ctaccacatg caagttctaa agaaggagta
     7981 aacaagcttg ctatgtcttt actaatagta gaagatgaag ttgatttatt aggaaaacca
     8041 gtaaatatat ttatggtatt agctgctgta gataacacta ctcacattag agcattagca
     8101 tcactatcag agatgatgta cgaggaagaa aatgtaaaac taataattaa tggtgataaa
     8161 agttcaatac tagaacttat aaataagcaa aattaggagg aatgaaaaat gaagatatta
     8221 gcagtatgtg gttttggtgt aggttcatca atggtattaa aaatgacaat acaaaaggct
     8281 ctaaagcagt taggtataga atgtgaagta gaaaatacag atataaattc agctagaggt
     8341 gctgattgtg actgtatatt cacaagtcaa gaattgttaa atgaattaaa aggaacttgt
     8401 aatgtagatg tatatccagt taaaaagtac atggatttag cagaagtcac agaagttgtt
     8461 caaacttttt taagtgataa aaatgataag aaatagtata agttttaaaa agtaaattta
     8521 agttaatcag aataaaataa aaacttacta aattcaaaaa tagtaagaaa gagaggaggg
     8581 atttagtggt agaaaactta aacagttgac ctatcactaa aaaatataat gcttgatttt
     8641 atttcaaata atattttacg taatccatct atgttaattg gattaatagc agcatttggt
     8701 cttgtactac agaagaagaa agcatctgat gttataaagg ggtcactact tgcagctttc
     8761 ggaattataa ttatggagac tggaacaggt atgttagtta gttcaatagc accaattaat
     8821 ggagctttcc aaagtataag tggtggtgca gtaacaaaag gtttaagtga tgttactttt
     8881 actgcaagtt atggtggaat tataggttta gcaatgtttg cgggtttagc tattcaccta
     8941 atgatagcaa gatttactcc tattaagaca atctttttaa caggtcacat gctttattgg
     9001 ttcccattca tatttgtagc ttcaggagtt gaagctggat taaatggtgg agtattaata
     9061 gcttttgcag cagtattatc agcactctat tggtcaataa tgccttggtt attaagaaaa
     9121 tatgtgtttg atgttatagg agatgattct tttacattag gtcatccatc tggttgttta
     9181 gcacttatat caggatttgt tgctaaatta gtaggaaata aagaaaaatc tacagaagat
     9241 ataaacatac ctcaaagttt atcatttttt agagaaatat ctataagtgg tggaatggca
     9301 gtgtttttaa tgtttatagt tgttgggata gtaataccag gtgcgtttaa agatagtaat
     9361 gagtttattg gaacgtatgc aataaagcaa ggtctttctt ttggagcagg tcttataata
     9421 atgcttcatg gggtaagaat gctaataaat caaataatgc cagcatttca aggtatatct
     9481 gaaaaagttg tgccaaatgc actacctgct tttgactgtc caatattatt taattataaa
     9541 ccaaatgctg tattaatggg attcttagtt gctatgctaa catctacagc tatgatttta
     9601 atatgtaact actttaatgt atttaatttg ctacttatac cacttgttat aacttcattc
     9661 tttgagtgtg gagcagcagc agtaatagga gaatcacaag gtggtatacg tggagctata
     9721 ataggtacag ctatagctgc aatatttatg gtagttttag taggaatatc tgctgtaatt
     9781 tactcaggaa ctattcaaaa ttggatacta atatttggag gtaatgacct ttcattattt
     9841 ggtataatca gtaggtttat aggttcacta tttggaggaa tattatagat atgaaattta
     9901 aatacataga tgagataaga agattaattg atataataga aaacgaagaa aaagtcaata
     9961 tggaaaaagc agtacaaatc tttgttgaag caatagaaag taaaaatgct atattttcat
    10021 ttggagcttc acatgcagga atattaacag aagaattatt ctatagagca ggtggctgtg
    10081 ttcttataaa tccagtattt gcaaaatctc taatgttaga tacagcgcct ataactcaca
    10141 caagtaagat ggaaagatta gaaggatatg gtacagctct agctgataaa actccaatta
    10201 aaaaaggaga tgtagttata gttcattcag tatctggtag aaatccagta agtatagagt
    10261 ttgctatgaa agctaaggaa aataaggcaa tagtaatatg tataactaat ttaagttact
    10321 caaaaagtgt atcttcaaga cattcttcag gaaaaaaact ttatgaagta tgcgatttag
    10381 tgatagataa tcatggtcaa aagggagatg cctgtgtaga gatagatgga ttaaaccaaa
    10441 aagttagtcc aacttctact gtaatagcaa ctactgtgat gaactctatt atagctcaag
    10501 ctactcagga attagttaat aaagggctaa agaatcctcc gattttctat agtgcaaata
    10561 ttgatggtgg agatgagtta aataagaaga tttttgatga atataaaagt gtaattcatt
    10621 atgaatatta aggttaaaaa aatatgttaa tgaactttga agtttatcaa caaagaatat
    10681 taataatata taataaatgc tatcatttaa aagctgtagg taaaaatcta cagcttttta
    10741 ttattgtaga ttaaaaagat gcgtaattaa attttaaatt ggagaaaata aattggaagg
    10801 tatcaattat aaatattaga aacgttgtca tagaaaaaaa ctatattaaa aaaaagtaca
    10861 tagaaaaaac tgattagaca ataaatacat gctttgataa tattttaaat ggagtgaaaa
    10921 tgtgtagaat tataatgaaa aaagaaaata aaaatgaaaa aagaaaataa aatggaggga
    10981 ataaaagtgg gtatgttaaa aaaattatgt tcactagcta tggtagcaat tatgactaca
    11041 acacttataa cagggtgttc aagtggaaat ggaaaagatt caaaaaagga tggcgaaaaa
    11101 gaaaaactag ttctttggat gccaccagta gaaaataata tgaaggaagt ttgggaccct
    11161 attttagata aatttgagga aaaaaataac tgtgaagtag atttacagat aattccttgg
    11221 gataattatt ctgaaaaatt tgcaacagct ataagtgctg gcgaaggtcc agatgtagga
    11281 tatatgtatg cagaaatgta tccacagttt atagaaagtg gagcagtaga agatttgact
    11341 aactatgcaa ctaaagagga taaagagcaa tctatttata taaaaacatc tgaaatgatg
    11401 ggtggaatgt atggtatgcc attccaagca gcaaatccag gggtattata ctataacaag
    11461 gatattttag agaaactagg agaaaaacct cctaaaacat gggaagattt taaaagaatt
    11521 tgtaaaaaag caacgcaaga tactgatggt gatggaaaga tagaccaatg gggattagct
    11581 cagggatggg gagctaaaac ttttggtaac ttaaactgga actggtatcc ttatttatgg
    11641 caagctggtg gagatatatt taatgatgat ttaaaatcgg ttaaatttaa tgataagagt
    11701 ggtttagaag cagctaagtt cttaaaagaa ttacaagcat atgtgcctga agattcttta
    11761 tctaaagata gtaatgaaat gatagaaagt gtatttggac cagggaaagc agcatttaca
    11821 ataatgttat cttctgctgc aacaagtgta tttgatgaat cattcccgga tttaaattgg
    11881 ggatttgtaa ctggactaga agataagaaa gcagcaactt ttggagcagt agaccattta
    11941 tctttaatct catctgcgaa aaataaagaa cttgcataca agttaataca acatatgtta
    12001 agtgttgaat caatgacaga atttcataaa gcaataccaa gagcaccaat aacaaaaggt
    12061 gaaccatatc aaggtgatga aagattcaaa gaaatggtgg aaaatgacaa aaatgtatat
    12121 agaccattag tagtaggacc tcatggagtt gaaatatatg aatatttatg gaaagaactt
    12181 caaactatga tttcaggaga taaaactcca aaacaagcgt tagatgatgc tgcaaaatat
    12241 tcaaatgatt tattagcaca ataaaagttt aaagctgtaa tatgttgtaa agtactttac
    12301 agattaaaat tagcattgta tttataaata caatgctaat atttatattg gagaggataa
    12361 aatggaaaac ttagttagag agtttgaaga aaattcaaaa agaaaaaaaa caaaattaaa
    12421 tattaaacca tatatgtata tattgccatt gggaataatt ttagtggcgt tttatgtgat
    12481 accaataata atgtcaatat actttagttt tacaaagtat aatattatta gtccagctac
    12541 ttttataggt ttagaaaatt ataaaaaatt atttacagat gaaacattaa aagtatctat
    12601 tataaatact attaaattta ctgttgttgt agtaccttgc caaactatat tatcattgat
    12661 tttggctgtt tggattacag gaaaaggaaa tagtaaaata gcttcatttg ctaaaggtgc
    12721 tatatttata ccagtattat catctatggt tttgataggt atggtatgga gggctttatt
    12781 gaatggagaa ggttctatta tatatcaggt attaggtgcc tttggaatag aatcatctaa
    12841 acttttagga gatagtaaga cagcattacc tacacttatg tttatatcta tgtggaaaaa
    12901 tatagggtat ttcatggtaa tttatatttc agctataatg aatttaccaa aacactgcta
    12961 tgaagtagcg aaggttgatg gggctacaaa acttcaagaa tttataaaaa taactgtacc
    13021 tttattgaaa ccaactacaa taatggttgt atttttaggg tcgatatggt cattacaagt
    13081 atttgactta atttatactg tgacaggtgg aggtccaggt atatctacta tgagtatagt
    13141 tatgcatgca tttaacttaa actttaaaaa ctttaattct ggatatgcaa tgactgtagc
    13201 aaacgtatta tttttgttaa tagcagtagt ttcgatttta caaaataagc ttgtaaaacg
    13261 agacaattca gatttttaga aaggtaggta taatatgaaa acagcaagaa gattattaag
    13321 tgatggttta cttttattta tagcgtgtgc aagtttggta ccttttatat acatgttgat
    13381 aatatcattg aagataacat ataactcata tagtttggat atatcttttt caacagtaac
    13441 ccttcaaaac tatatagata tttttactaa aaaaggattt gcacaatatt tttttaatac
    13501 agcaatagta tctttttcag gtgtattatt aaatttagta tttagtacat tagcaggata
    13561 ttcttttgcg aagatggatt ttaaaggtag tgataagtta tttttattta tgataatgac
    13621 tttaattata ccttcccaag ttacaatgat accactatat ataataatga aacacttagg
    13681 atggataaat agttatcttg cacttattat gccaatacca actgcatttg gcgtatttat
    13741 aatgagacaa gctattttag gagtaccaaa agagttatta gaatcagcaa agatagatgg
    13801 ttgttcggat tttagaattt taatacaaat agtgttgcct ttaattaaac cagcattaat
    13861 tacactagca atatttactt ttatgggagc atggaacgag tttatgtggc ctcttatagc
    13921 tacaactaaa gatgcaatga gaacattaac agttggatta tctactctta agacattcca
    13981 gataactaat tatggacaga tgatggcagg ggctacaata acatttttac ctccatttat
    14041 tttctatttg attttacaga gtaaatttgt tgaaggtgtg tctttgtctg gtataaaagg
    14101 ttaaatagtc taatttaata atatataaag aaacttatat aaatatggag gaatttaaac
    14161 tatggaaatt tcatatgtac tgaaagattg cacattcaaa cacaataaat actcagttgt
    14221 agaagctgaa acttggaaag ataaggattt ggatattgta gtagctaaag gtgaaaaatt
    14281 tgcatttcaa attatgttaa aagcaacaga agaatttaat tgtacaattg ataaaagtag
    14341 tgctatatca tggaaaggcc tgggaaatag agtaaggtta gcattaaata tgcctaatag
    14401 tttagaaaat aattttagta taaatatatt aggatatgtt caagatgata caaaggcatt
    14461 tgtgaatgat gctattttaa gggataaaga tgtgttagta gagcaatatt tgcctcaaac
    14521 attttggatt gaaggtcaag taccagaaga ttttgaagaa aataaccttg atataggtat
    14581 tgatatattt aaaagctttg gatatgaaga tgaagaaaaa gtttgtacaa ttgatgttcc
    14641 tgtaaaagta agaaatgttg tgctaaagcc tttagatgaa agcaagttct ttttagattt
    14701 atggcaacat ccaagctgtt tagctagaat gtataaagtt gagttatggt ctgatataca
    14761 ttttgaaata gtcgataact atttaaaaga attagcatct ttaggagaaa aagttgcaac
    14821 agttatagtt tcagattatc catgggcagg gcaaagttgt tataaagttt ataaaaatcc
    14881 atcaaactta tatgaatata acatggtaag tgtatcaaaa ggattggatg gaaagattaa
    14941 atgtaacttt gagagtatgg ataggtatat aggcatagct gataagtata aaatggctaa
    15001 ggagatagat ttatttggtc tattaggtaa ttggtgtgct ggtgagtttg gaaatcctgt
    15061 agaaggttat aaggacccta taagagtaag atattttgat gaaaaggata aagtatttaa
    15121 atttataaac aatacaaatg atttaaaaga atatatagga ttagtattaa atcatttaat
    15181 agagtgtgga ttatgggaca gagtaaggat tatagctgat gaaccaaata atccagaagt
    15241 agtcaaagag tgtatagaat ttataaattc tactgtaggt actcatcaag ttaaatataa
    15301 atcagcaact cacgaccaaa actttttaga cagagctaaa gatgaaattg atgatatgtc
    15361 aataaattta aaacttacta tacaaaatta taaagatata gaaagtctta agaaaaaaat
    15421 caatgacaag ggtgggatat tgacttggtt tgtatgctgt tttccagaaa aaccaaatag
    15481 ttttttaagt tctccttttg tagaaaatag aataatagga tggtatactt attactttgg
    15541 tcttgatgga tttttaagat gggactataa tttatggaca gaagaccctt ggaaagattc
    15601 aagttacaaa ttccctattt ggaaagctgg agatatgttc tttgtttatc ctggaaaaga
    15661 tttaaagcca gttagaagtg tcagaatgga gaatttaaga tttggaatac aggattttga
    15721 gttatttaca atgttagaaa aagaaaaggt aagagagtat atagaagttg agcttatgca
    15781 agaattatta aataaaaaag aaaatgctga aattaaaggt tttggtgaca tagaactagg
    15841 atattcactt gataattgga gatatgataa agttaaaaaa tatgtattag agttgttaga
    15901 cagagtataa aaaatataag agtgtctcaa aatgaacttt ttagttcatg aggcactctt
    15961 tttgtatgaa atcaatatag tttcattgtt tcaacaatga aaaataggat tatttctatt
    16021 ctaagacagc cctttagttt tttagttgta ttcaatgttt taaaactaac tataaacgct
    16081 ataaagttta taaatgacaa taattatata taacatgatg tactttaatt tgctataata
    16141 gggattgaat aatattatat ggaagataac attgtttggg gggattaata taaaaagaat
    16201 aggtattatt ataatgtttt ttttaagtat atcttttatt ggattttatc ttgtaataag
    16261 agataacagt gatacagtta agatgcaacg agggataaat ataggtaatg cactagaatc
    16321 acctaaggat tttccgtggg atgtaaaaat gtcaaataag ttttttgatg atataaaaga
    16381 tgcaggtttt gacacagtac gtataccagt aaggttttct gattatacgt cagattctga
    16441 taattttaaa attgatgaag aattttttaa aaagattgat aaatatgtag attatgcatt
    16501 ggataaagac ttaatagttg tgttggattt acaccatttt gaagaaatta tgaaagaacc
    16561 aagagttcat aaagaaaagt ttttaaaaat atggcaacag atagctaata ggtatcaaaa
    16621 atatgataaa aaattggtat ttgaattatt aaatgaacct aaagaaaatt tatcttcaca
    16681 attactaaac gaatacattg aagaagcaat aaagataatt agaaaaacta accccaaaag
    16741 aactattata gtaggaccat ataatttcta tcaaattgat tatttgaatg aattaaatat
    16801 tcctaaagat tctaatatag tagtttcttt tcactattac gagccaaatg attttgcatt
    16861 tcaaggaaat atctatcata agggatttga gcacttaagc aatattactt gggaaggaac
    16921 aaatgaacaa atggattatt taaaaaaaag gtttgatact gtggaaaatt gggcaaataa
    16981 aaataaggta aaaatcttct tgggtgaatt tggaataaca aaagaggcac cagaagtttc
    17041 aagaagagca tggataaaag ctgtaagaga agaagctgaa aaacgaaact tttcatgggc
    17101 ttattgggag cttgcatctg gatttggtat atacaatcaa attgaaggga cttgggatag
    17161 agatatatta agtgcattaa tagaaaagag gtgattttat gaagaaaaat tcaaaaaaga
    17221 tacttataac tttaactatt attactaatg tcatatatat attgtggaga attttttaca
    17281 cagtacctaa agaagaaggg aagtttgcct taatatgtgc cataatactt ctttttgtag
    17341 agataatggg tatgatggaa atgtttgtgc attattatgg tatgtcaaat attgagtatc
    17401 ctgaaaaacc aatcataagt gaagaacttt atccacatgt agatgtattt attgcaacat
    17461 ataatgaatc tgttgatttg gttagaaaaa ctgtaaatgg atgtattcat atgcagtatc
    17521 cagataagaa aaaggtacat atatacatat gtgatgatgg aaatcgtgaa gaaatgcgta
    17581 ttctagcaga aaaaatgggt gtaaattaca taactagaac tgaaagagaa ggagcaaaag
    17641 ctggaaattt aaataatgca atgcaacata caacttctcc tttgattgct acatttgatg
    17701 cagatatgat accaatgcat gactttttaa ttgctacagt accatacttt ttaaaaaatg
    17761 agcaagctaa aaaagatgga gaaaaagagg agtatgaaaa ggttggtttt gtgcaaactc
    17821 cacagagttt ttataatcca gatttgttcc aatttaatct gcactctgaa ggaaggattc
    17881 caaatgagca agattatttt tacagagata ttcaattggc tagaaatagg acaaattctg
    17941 taatttatgg tggtagtaat acagttattt caagggaagc acttgaagaa gttgatggat
    18001 tttatactta ttctattaca gaagattttg caacaggtat cttaattcaa agtaaggggt
    18061 atagatgtta tgctattcca gaagtacatg catcaggttt atctcctaca gatttaaaaa
    18121 gtcttattaa gcaacgtgaa agatgggcac gtggatgtat acaaactggt agacgtctaa
    18181 atattttgtt taggagaggt ttaggttttt ggcagaaaat aagttatatt tcttcaatta
    18241 cttattggta tgcaagtata aaacgctttg tatatataat ggcacctatt ttattttctg
    18301 tgtttaatgt gattgtagta aaatgtacgt tacttcaagt attggtattt tggttgccaa
    18361 tgtatatttt aagtagttta tctcttaaaa tattttctca aaatataaga aatactagat
    18421 ggaccaatat atatgaaact ataatgtttc agtctttaat gccagcagta atacttgaaa
    18481 cgtttgcaat atctaaaaat aaattttcag taacaaataa gagtaaatta gaagaaaata
    18541 gaatgtataa gtttttacag ggaatacctt attttattta catggtctta tctatcatag
    18601 gaattttaaa aatgtttgta gcaatattta aaatgagttc aatgacatat tcagttgtat
    18661 tattttggtt gattggaaac ctttttaacc ttgttatggc tacattattt atatcaggga
    18721 gacagcagtt aagaaagtct gagcgttata ttgctgaaat tgattttaag ttaaagcaaa
    18781 attcttatgt tctttcttca aaaacaattg atatatcaga aaatgggttt gcatttttgc
    18841 tagaaaatcc agaatatatt tcaccagaag aagagtttga ggtagagttt agagaaaaat
    18901 ctggaaatga aatgtatata gccaatatga aagcaaagat tgttaatgtt gtggaagtaa
    18961 attcaaagtg gaaatatgca gcttatatta cacatattga agatagtgaa atagataatt
    19021 ggatgtgtat tgttcacgat agaataccta ctttaccaat gacaatatct aatcaactag
    19081 gtttctttga tgatttgcaa attaatgtga aaaaacgtat tgaaaaaact agaacattat
    19141 cacgaagaag tccaagaatc aatatgaatt ttcagatgga tataaaaaat acaggtaaat
    19201 taagaatagt gaattttaat tatcaatatg tattattaaa ttttgagaat aaaagtattt
    19261 atcctaaaga aatagcattg gaaattaatg aaaatattgt gttagaatgt gatttgtgtg
    19321 agggtaaaat tgatgaaaga ggtatcttat ataaagtaaa taacattgac agcataatgc
    19381 aaaatttctt tttaagagat gaaatgatgg attggatatt acaaaataag acaattcttg
    19441 tttcaaagcc tagtgaaaag aaggaaaaaa gtatagatga gtttgaaccc atggaatata
    19501 tttaggagag atgaaatgaa gactttaaaa gtgttttttt gttctacaaa atatcttatt
    19561 ttggtgttta ttttttttat aaatactact tttataagtt cagcaacaag tgataaaatt
    19621 ttagatttgt cctttaaaaa gatagagact gacttaaatt ctaagattac atatgaagat
    19681 acaggtgtaa aaatagaaac ggatagttct aaaagcgata aggaacgtta tttatatatt
    19741 taccaaaata taaaagaaaa ttggagcatg tataataatt tttatattga aattcaaaat
    19801 aaaaataaaa gttctcagag aataaattta agcatacaaa gtaaaaatat gcttgaattt
    19861 agattaaaag aaggtagtga agtattttta gaaggtaaaa atataatata ttcagataaa
    19921 ataaaagaag gatgcataga agtaccaggt gaatttgaag gcaaaattta tgtgaacttt
    19981 aatagcctta taaatgagga aagtaatgtt gtgctggact caaatatgct ttcaaacatt
    20041 gttagttggg gaattacgtt tattccatct gatgaagaac ataatattgt tattataaaa
    20101 aagatatctt tattgtctga agagaaatta agatttttaa ataatattaa aataattggc
    20161 gatgaagaag tacaaatacc agtattgggt caaagtataa gtcagtatga agtattaggt
    20221 cttaaaagtg attctaaaat taagtactct cttatgggaa aacaagataa tgtaagcata
    20281 tctcaaaaag gtaaattaac attaagtaat aaagccaaac ctggacaaat tattttacaa
    20341 gttaatgttg atgataagtt taaaattgga aagaaaataa cattaactga gtcttggagt
    20401 gttaataaaa aagataagga tggtgttcca tatacattag tatatcctga gcaatcacca
    20461 acagtacaag atatgaaaaa aataaacttt atgaataaca tcataacgtt tgttcgaatt
    20521 ttatttgtat ctctagtaat aatatgcttt ggaatatatt tatattggaa aaaatgttca
    20581 aaaactaagt aaagagagga ggttagattg tgttattttc aagtttaatt tttttatttt
    20641 attttttacc tataactttg gttttatatt atgtatttag gtttaaccga acgatacaga
    20701 acatgatatt gcttgctgtt agtttatttt tttatgcttg gggagagcca aaatttgttg
    20761 ttatcatgat agtttccata ataatgaact atatatttgg tttactggta gatagatata
    20821 gggaaagtaa aataaaagta aaagtttttt tagttttgat gtgtgcatac aatataggtg
    20881 ttttatttat atttaaatat ctagcttttg cgctaagaaa tattagtgaa cttattagta
    20941 ctgaattgac tatacctagt attgttttac ccataggaat ttcatttttt acatttcagg
    21001 gaatgtctta tgttatagat gtatatcgta gacatggaga agtccaaaag aacccttttt
    21061 atgtgggatt gtatatagca tttttcccac aattaatagc aggtcctata gttagatatg
    21121 agagtgtggc agaacaaatt ctcaatagaa aagaaacttg gaataaattt tctattggga
    21181 cttgtaggtt tataacaggt ctaggtaaaa aagtattaat ttcaaataac atggctatag
    21241 tagcagacta tatttacact atgaatagtc aaggagaaat agctgcatca cttgcatggc
    21301 ttggttcaat agcctatact ctgcaaatat tctttgattt ttctgcttat tcagatatgg
    21361 ctataggctt aggtcttatg tttggtttta aatttgaaga gaactttaat tatccatata
    21421 tatcaaaatc aattagtgag ttttggagaa gatggcacat ttctttggga atgtggttta
    21481 agagctatat atactttccg cttggaggtt ctagagtagc aaataaagat attatgatta
    21541 gaaatatgtt tatagtgtgg ttatttactg gaatatggca tggagcagaa tggacatttg
    21601 taatttgggg gattttaaac tttgttttcc taattataga gcgatttata atgtttgaaa
    21661 agattgaaaa tcacaatttt ataaaacata tttatgcttt attagtagtt aattttgggt
    21721 gggtattatt taggtcacct aatttaaaag aagcatataa ttattttaaa gctatgtttg
    21781 gaggcaatgg agctatatgg agtgattata catatatgtt tttaaaagaa tactgggttt
    21841 tctttatatt tgcatttatt tttagtgtac ctattgcaaa gaaaatcaat aagtttgttg
    21901 ttgaacaagc taaatatagt atgatattta acgtatttta tccaatctca gctattttat
    21961 tgttttttat ttctgtgaca tatttagtaa ctggaagcta taatccattt atttatttta
    22021 atttttagtt gggaggaaaa gtatgaatag attttttaaa gtaagattaa taactgcaat
    22081 tatgttctta attattttat tcgctttctc tatctttaat gtttgttatt ctggaaaaga
    22141 tacgatacag gaaataaaaa attcaatagc tgaaaaaagt agtttaaaag atacaatatc
    22201 atctattgat aatacagtga atgacaaagt aatatacaaa aatacgttca ttgaaactta
    22261 tggctattta caaaaattaa tgggtaaaaa tgagttttca aaatttagtg ttgtaaaaga
    22321 tacttctgga aagatgcatt atacatattt tgctaaagct ccaaatccag ttggtgtgat
    22381 agcagatagg gtaagaaaat tttctgatga aatggaaaag caaaacacta aattggttta
    22441 cctaatgact ccagataagt atataaaagg agtgacaaaa tttccaaaag gaatacctta
    22501 ttcttatagt aatgagacag cagataattt tttaaatcaa ctaaaaaagt ataatgtgga
    22561 ctatattgat tttagagaaa atattttaaa gagtggtatt ccaaaagatg atttattttt
    22621 taaaacagac catcactgga aagtagaaac ttctttttgg gcatttggag aattagttga
    22681 gcaacttaat aaaaaataca atatgaattt agatgaaaat cattattata gagataagga
    22741 caattataat tcaattgtat atcctaagtc atttcttggt tctatgggaa gaaagactgg
    22801 tatcttatat ggtggtatag atgattttac attaatttat cctaaattta agactaatta
    22861 tacttattat acagattcta aatctcaaaa atttgaatta aaaggaaggt ttgaagagag
    22921 tttaatatta tcatatcctt ttaatgcaga ccttgattta atggatggtc aatcagataa
    22981 gtattttaca tatttattgg ggaacagacc attagttaaa ataaaaaata tagaaaatcc
    23041 agatggatta aaagtgctgt ttgttaaaga ttctctaatt gtaccaacag cagcattttt
    23101 ttcctctgtt tgttcatcta tagatatgat agaccctaga tattatgatg gagatatact
    23161 agaatatgca aaatcccatg actatgactt tgtttttata tctgtatatc cccaaaatct
    23221 tacaaaagaa ttttttccgt tttgtgagta aatttctaaa aaagttttct gataaaataa
    23281 atggctaact ggtaaaaagt tagccattta ttttgagtgt tattctaaga aatatttttt
    23341 ttactaaata cttctttatc tatttttcca agtgattttc ctacagtaat tgatgatacc
    23401 atagcaccat taacatttat catagtacgt cccatatcta gtatagggtc tattgcaagt
    23461 ataccaccaa ctaatggaaa ataagaacca agaccaactc cagagataac aactgaaacg
    23521 gccattgtag cagttcctgg aagaccagct attccaaacg aactaattgt tataacaatt
    23581 aacaacataa cataaaagct catatccatg tttactccag ccatattggc aagtgttaca
    23641 gccattaaag caggatatat acctgcacaa ccattcatac ccatatttgc acctaaactt
    23701 gctgtaaagc ttgatacacc ctcatcaact ccgtgatttt gtactagact ttctatcgtt
    23761 acaggcaatg ttcctaaact agaacgtgaa gtaaatgcca gaagtaaagc ttcagttgaa
    23821 ttttttatat aagtaatggg atttagacca tttaaagtta taaaaattaa atgtataata
    23881 aataatattg caactgatat ataaagagct aatataaact gtaaaacaga tactattgaa
    23941 ctaatacctt gtgaagttat tgtatttgca agcagagcaa ccacagcata aggcataaat
    24001 ttaataacag tcatagacac acttacaatt attttgtaag atgcttctat aaaacctata
    24061 aatggtttta ttgtttcaaa atgtttttta cttaatctct ttattgaggt agctataaat
    24121 gtagcaaata ttattgtagc tacaatattt ccatttgcca tagccattac aggattagat
    24181 ggtagtaatc ctcttaaagt atccacaaga gagtttattt ctttaagttc ttgattttca
    24241 actaaagtag gcatattagc tcctacacct aacttcatta tatttgcaac gataattcca
    24301 acaattgcag ctatagcagt agtagcaaga agcataccta aagatttaaa tgtcagagtt
    24361 ccaagattgt tatctttcat atttataatt acttttataa tggataagaa aactaaaggt
    24421 actactaaca tttttaataa gtccataaat ccagaaccaa ataggctata ccacttactt
    24481 acttcttgta gccaaatgac ttgagttgta tcattaggaa aaccagcaaa agcttgtatt
    24541 atgattccca atatgagtcc tagtactgta gcatatattg ttctcaatga aaattttatt
    24601 tttttctttt caaattgtct aacaataaag aacgtaccta gaagtaatat tatgaaaaaa
    24661 attgttttaa aatctgtaat cataagaaat tgtgataaaa atgaactttc caatattttt
    24721 ctccttttat ttaaaaataa atttttctaa gtttaaatga tatcaatata ttaaaaaaat
    24781 gtcaatatta tatcacaatt tacttaaata gagtaaaaca agttgatttt gaaaaatgtg
    24841 attaagtaca gaaaaatagg gtataataaa tgtttaaaga atttgaattg tggtaagaaa
    24901 ataaaaaata gcttttataa gatttgattt caaattaaaa tgtgttaaat aaataactaa
    24961 aaattattat aaaaaatgtt ttcatagaat atatatcaaa tgaaaagctt aaaataagtg
    25021 aagaaattaa ctagacatca aaggagagat aaaatgagag taataataat tggtggtgtt
    25081 gcagctggta tgagtgctgc agcaaaatta aaaagaataa aacctgagta tgaagttgtt
    25141 gtttatgaaa aaactgaaat agtatctttt ggtgcttgtg gattaccata ctttgttggt
    25201 ggatttttta atgatgctga tgaattatta gcaagaacac cagagaaact tagagaagct
    25261 ggaattgatt taaatatatt tagagaagtc gttgaggttg atagtgaatc taagaaaata
    25321 aaagtaaaaa acataaaaac agatgaaata tatgaagatt actatgataa attgatgata
    25381 gcaactggag caagaagtat tatgcctcct ataaaaaaca taaaattaaa gaatgtttct
    25441 acattaaaaa gtttatatga tggagaatat ttaaaaaaac ttttaagtaa tgaagataat
    25501 aaaagagtga caataatagg agctggtttt atagggttag aagcagttga ggcatgtaag
    25561 aaattaggta aagatgtaga tgtaattcag ttagaagata gaatattgcc acaagtattt
    25621 gataaggaaa tgacggatgt attagaagaa gaaataaata gacataatgt taatttgcac
    25681 ttagatgaaa tggttgtaga actttgtgga gaagataaag tagaaaaagt tataacaaac
    25741 aaaggggaaa tagatacaga tgtagtaata attgctactg gcgtgagacc taataccgag
    25801 tttttgtcaa atactaatat aaaaatgcta aaaaatggag ctatagtagt tgatgagtat
    25861 ggaagaactt ctgtagaaga tatatactct gctggagatt gtgcaactat aaagaatata
    25921 gtaagtaatg aaaatgtata tgttccttta gctactggag ctaataaatt gggtaggata
    25981 gtaggagaaa acctagcagg aagagaaata tcttatcaag gttcactatc ttcaagctgt
    26041 ataaaaatta tggatatgga ggcagcatca actggaatta cagaaaggca agctaaagat
    26101 ttaggcataa atgtcaaatc taaatttatt tctgattata atcaaactca ttattatcca
    26161 ggaagaaata aaatatatgt aaagcttatt tatgatgcag atacaaaagt tattttaggc
    26221 ggacaagtgg ctgggtttaa ggatgctgtc caaagagcaa atgtattggc agctgctatc
    26281 tttggaaaaa tgaccacaaa tcaacttgga atgttagatt tatgttatgc tccaccattt
    26341 gcaagaacat gggatgtttt aaatgtagct ggaaatgtat ctaaataata tttagattat
    26401 agctaatata gttgacaagc aattaaggga atgaatttac ctaaactatt tttatcatgg
    26461 taaaattcat tcctaaattt ttattccaaa tttttattcc aaatttttag aaataaggat
    26521 tccattaaat tattcatcaa aatttaaacg caagcttaat ctatcttctc caagaatagt
    26581 attttcaaaa acattagtag cattttctaa atacatacta ggtatgtcta aagaaggact
    26641 aaaatgagtc agtaaaagtt gtttgacatt tccaagtttt gcaagattag cagcttctct
    26701 aaaagtcata tgtttgtttc taacagcttt tgatatatct aaatcatcac catacatggc
    26761 ttcacatata aataaatcac tatcttttat gaaatctggt attgattcta taggtcttgt
    26821 atcggttgta agacttatct taactccctt tctcaattct cctaagacca tatctggagt
    26881 gtattgtttt gaatctaaaa ctatattttg accttcttgg agtttgttcc ataatatttt
    26941 aggaacttcg tttttagttg ccttatctat atcaaattta gggtttcttc taaaattaaa
    27001 actgtatcct aaacactcta tagagtgttc taaactgatt gttgaaattt ctaaatttct
    27061 taaaacttta ttatctaaag aaaaattgcc ttgagggttt tctataattt ttaaagtgta
    27121 tggtacatat tctactaaat ttctcataga acgtatacaa tcgactattc caactggacc
    27181 aactatagtt aaatcacttg tacgaccact attcccaatt gttgataaaa gtcctaaaag
    27241 tccaaatata tggtcaccat gtaaatgggt tatacaaatc aagtctatat ctttaaagcc
    27301 acattttttt agcttcatag atacctgagt accttcacca cagtcaatta gtatttttct
    27361 acctttatag tttataaata cagaggatag aaatctgttt ggcataggta catttccacc
    27421 acatccaagt aaagttaaat ctatcatttt ttcccctttc taaaattaac acaaatctgt
    27481 tgtttgtttt tattatgata tcctattata attattttaa cattaaatat aacaataaat
    27541 gtgtatttga gtcaagaaat attgtcaaat aaagttttta tatagcaatg tataaaaatt
    27601 tggaaaacga aaagtgaaaa ttatatttag gagtatttaa aggaggaaaa tttaatgaaa
    27661 gtattaaaga aaattactat tttgtcatta attacattaa ccttagttgg ttgtagtaac
    27721 ggagaagaga aaaaagtggt tgaaggttct aagaaaactc aaaattctaa aaaagatgaa
    27781 cagcctataa tagtacataa agatgcaaat atattgaaag ataggtatga gccaccaaaa
    27841 ggatttaaaa gagttgatgt agaaaaaggt agttttgaat catttcttag gaatactaag
    27901 ctaaaaaatt atggagagaa agtaaagtat tatgatggaa gaactaagga atcaaaaggt
    27961 atttatgata gtgtatttga tgttgatatt ggaaatagag atttacatca atgtgcagat
    28021 gcaattatgc ttatgagagc agaatacttg tatcagaata aactatatga tgaaataagt
    28081 tttgattttg ttgatggttt taaggcgaag tattcaaaat gggcacaagg atatagaatt
    28141 tcagtaaaag atagtggttc aagctggtat aaagcaactg aatattcaac atcttatgag
    28201 agtttcagaa agtttatgga tatagtgttt gcatattcag gaacgttatc tcttgaaaaa
    28261 gaattggagc ctgtaaaagt tgaggatatg aaaataggag atgtgtttat agttggtgga
    28321 agtccaggac atgcaagtat aattatggat atggcagaaa atgaaaagac tggtgaaaag
    28381 gtatttatga tagcccaaag ctatatgcct gcacaacaaa cgcaacttct tattaatcgt
    28441 aaaaatccaa atttgagtcc ttggtatagt ttagattttg aaggtgattt gacaatacct
    28501 caatggactt ttaaaagaga ccagttgaaa agattttaaa ataataattt tatataataa
    28561 aagaaaaata tagatgtgaa aaaatgtaat tagattaaaa ctaattggat atggacaaaa
    28621 ttaaattgat agataaatat taaaaattac actaattaat cttactatag tttaatattt
    28681 atctaaaaag ttttatgtaa aatataaatt ttatattaaa gctaaaacta atttgaggag
    28741 gctaatttat attgaagatt agaaggtatt taattttggt aattataatt ctaattacaa
    28801 caagtcagag ttttatttct tatgggctag agcgatttga agaaataacc atttttcaca
    28861 ctaatgatat acatggaaga tatgctgaag gtgatgacca tatacaaata ggaaatctag
    28921 ccacattgaa aaaagaaact ccaaactcaa ttttagtaga tgcaggagat tgtttgcatg
    28981 gacttccaat agtaaatatg gatagaggga aaagtgcaat tgaattaatt aaatgtgctg
    29041 gatatgacta tattactcct ggaaatcatg actttaatta tggaaaagat agactgtttg
    29101 agttatctaa aagtgctaat ttaggagaga atactttaaa atttttatca tcaaatattt
    29161 ttgaaaatgg gaaaaaagtt tttgatagta atgatataaa agaaatagat ggaataaaaa
    29221 taggattttt tggactctca acccaggaaa caaagagcaa aactggattt aaaaatgtgg
    29281 aaggattaga gtttaaaaat cctatagaaa gtgcaacaga acaagtaaat gaattaaaag
    29341 gaaagggtgc caatgtaata gttgcaatat gtcatattgg tacaaatagt ttaagtaagc
    29401 caaatagtat agatatagca aatgaggttc aaggaattga tttgattatt gatggacaca
    29461 gccacactaa gtttgaaaat ggtaaacaag tgggtgatac attgattgta agtgcaggac
    29521 aatatttaga aaatattggc gagataaaac taactttgga tactaatgat gtagaagata
    29581 taaaaataga aaataaatca gctagattaa ttggaaaaga agaagcatta aagtataagc
    29641 ttgactccgt tgtaagtaaa aaggttgata aaataaaaga acaacaagaa aaaatattag
    29701 ataaagttat aggggtaact acaaatactc tagatggttc atatgaaaat gtaaggacaa
    29761 aagaaactaa tttgggtaat ttaataagtg acattttact agataaaact aaggctgata
    29821 tttctttatt taatggaggg aatattcgag atacgataga aaaaggcaat ataacaagaa
    29881 gagatattgt agatgtattt ccttttagca atactatagt taccaaagaa cttacaggag
    29941 cacagattaa agatgtattg gagcatggag taaaacttta tccagaaaaa agttcagcct
    30001 ttttgcaagt gggaggaatt tcatattact ttaatcctaa acaaaaagaa ggcgaaaaaa
    30061 taacaaatat tcaaaaagaa ggaaagccat tagatttaaa taaaaagtat gtagttgcaa
    30121 cgaatgatta tatagcatca ggtggagatg aatttccttg tttttcaaaa gagccagtac
    30181 taaaggaatt tggaaattta gagagtgcag tcattgaata tatagagtac aaaaaagaaa
    30241 tatcaaaaaa tgtagatgga agaattggta ttaaatctaa agatgagtta ataagcaaga
    30301 atcatgagag tgatttgata ttaagtgaaa atgaaaaaaa tatcaatgat aatgaagaga
    30361 atctatctaa taatataaaa agctctgata gtgataaaaa taaaagctta gataaaacaa
    30421 gttctagaga aagttctaat ctaggaatat ctaaaagaga agctaaagaa aaatctccta
    30481 aaacaggaga tttaggtttt tcaaatagta taattatatt tatagtttca agtacattga
    30541 tatgtttatt gaattttaat caaaaagagt taaaggataa aaagtcaaaa taaaaattaa
    30601 tgagcaaaaa ttagtagata gtaatttaat tttagataat agtaaacagc aaaggaaatt
    30661 agttatataa atactaattt cctttgtttt atgttttaac atgattactt ttaaataaaa
    30721 ttgtatttta tgaaaaataa ttatactaaa tggcatagta ttatggggat ttttagtttt
    30781 tattcaagta aatgaaatca tattaacaac ttctgatata tttacaaatt gataaaaaaa
    30841 tgattttatt agtatgatat aatttagcta gtatgtatga taatggagga tatagggatg
    30901 aataatatat tacttttgga agatgacaaa tctttaaata gaggaattag ttttaaatta
    30961 aaaaaagaag gatacaatgt ttttagtgca tttggtatag aagaagcaaa aaatattttc
    31021 gcaaaagaag agatatgctt aattatttcc gatataggtc tacctgatgg tagtggtttt
    31081 gacttttgtg aggagattag aaagaaaagt gatgtttaca taataatgtt gacagctctt
    31141 gatgaagagg tggatattgt cacaggatat gatttaggtg cagatgatta tatcacaaag
    31201 ccttttagct tgatggtatt aatatcaaaa gtgaatgcgc ttatgaaaag agtaaataca
    31261 gtaaaaaatt atactctatt agtttgtgat gatttattct tttattatat agaaaataaa
    31321 ttaattataa gaacagatga taaggaagaa gagataattt taagtaagac agagacaaaa
    31381 ctattaaaat atttgatgga aaattctatg cagacattga ctaaagagca actgttagaa
    31441 tctttatggg atagtagtgg taattttgta gatgataata cgattgcagt aaatataaga
    31501 agacttagac aaaaggttga aaaaaatcca tctgaaccta aatatataaa aacagtaaga
    31561 ggggttggat atatgtgggg agaaaggagt ataaaaaaat gctaaaaaat atgaacctca
    31621 aagatggttt aagtgtgaaa gatgattata taatgagaag agttattgta tattctgttt
    31681 tatctgtact aatagtaacc cttgtattta tactttttga atatagcaga ttacataaat
    31741 tgtatgaaca tcaaataaaa aatactcaaa atgttgctgg aatattgatt gaaaaatatc
    31801 ctaatgatga aatagatata gtaaattcta tttataattc tgattatagt aaggtgtcta
    31861 ctggagaaaa ggcatttaag aaatttggct atggattgga aaataaaatg tcaaatgata
    31921 aaaattttag cttatactta aaatcttttt ttaaggaatc attttttatt tttttaatga
    31981 tgtttatagt agttataggt ataatatatt attttataag atacataaat aaaagattga
    32041 gtaaaattta ttttatagta gataagatgt cacaagaaaa ctacttgaag gacactactg
    32101 agtcagaaga aactggttat ctagtagaat ataatgaata ctttaaagaa ggcatattta
    32161 gtaagataaa taactgtcta tatgagctta atagaagttt aaagataaag tttattaaat
    32221 tagaaaaaga aaaagaaagt gtaaaatcat tagtaacaga tatttcccat caattaaaaa
    32281 ctcctttagc atccttaaaa ttatacaata cattacttat agaagaagaa ttagaagaag
    32341 atgaagccaa cgagttctta ttaaccaata aggcttctat aaataaatta gaaaatttaa
    32401 taaattcttt agtgaacata tcaaggttag aaattagcat gataaacata aaaaaagaag
    32461 ataatgatat aaaaaatact attctaaatg cagtaaaaag tgttacccct aaggctaaga
    32521 gcaaaagtat aacattaaat ttaaatgaat ttaacagtag aattatacca catgataaaa
    32581 agtggactga ggagagtatt tttaatgtat tagacaatgc tgtaaagtat acacaggtaa
    32641 atggagaaat aaatatatct gtagaagaaa ctgctaattt ctttaaaatt ataatagagg
    32701 ataatggcat agggattgaa aaaagtgagt ttaataatat atttaaacgt ttttatagag
    32761 gttctgctga tgaaattgaa tctgtagaag gtagtggggt aggattgtat ttaagtagaa
    32821 agattttaga agaacaaggt ggaaatatta tagtttcttc aaaagtagga atagggagca
    32881 agttttcttt gtttcttaca actatgtaat attaaatgaa aggaaaatgt aagcctctac
    32941 atttattata taagtataaa cagctccaga aataaaaaat taataaaata tggagtaata
    33001 taacttagaa tgagaggata attttatgaa aatactatat actgaaaatt tatcaaagca
    33061 ttatggaaaa ggagaaagct tagtaagagc tttggacaat gttgacttag aaataaatga
    33121 aggtgaattt gttgcaataa taggaaaatc aggttctgga aaaagtacac ttttgcatat
    33181 gattggaggt cttgatatac ctacatctgg gaaggtgtat atagataata aaaatatatt
    33241 tacattaaaa gaagaagaac ttgctgtatt tagaagaaga aaaatagggt ttatatttca
    33301 aagctataat ttaattccat ctcttaatgt atgggaaaat gtagtgttgc caatcggttt
    33361 agatggtaga gaggtagatg aaccttttat aaaagagctt ttaaaatcat taggacttga
    33421 aaataaacac gacgttttac caaatacatt atcaggtggt caacaacaaa gggtggcaat
    33481 agcaagagca ttagctacaa gaccagctat aatacttgct gatgaaccaa ctggaaacct
    33541 agattctaaa acatctgatg aggttatgag tatattaaaa tctatgtcta agaagtacag
    33601 tcaaactcta gttatgataa ctcatgatga cagtatagcc caaatggctg atagagttat
    33661 attcatagaa gatggcaggg tgtcaaaggt tggtgataaa aatgattaat actactaaaa
    33721 tagcaaaaag taatttaaag caaaataaat caaaaagtat tctaataata attactataa
    33781 tactttcaac cttaatgctt tcttctattg gaatttatat tgttgatgca ggagcatatc
    33841 aaaaagaaaa tactataaag tattcaggta attatcaagg tatattagca aatgtagacg
    33901 aaaaacaagc tgatatattg agtaatcatg cagatgttga attaactggt aaaatgaatg
    33961 gagtaggtgt tgaaaaactg gaagatgatt caaatatttc tctagcatac atgaatgaag
    34021 atgctttgaa attaaatagt tttgaattta tcaaaggaaa attgccatca aaagagaatg
    34081 aaattgtgtt agatagtgga gctttaaagg cacttggtta taaaaataaa gatttaggtg
    34141 aaaagataaa aatatcttat aatgactata aaagtgataa aaaaattgaa aaagagttca
    34201 ttataagtgg aatcttaaaa actagcgaaa tatctgaagc aggtaaatat tattatgcta
    34261 ttatatcaga atcatatatg agaaatacta gagatatgtc acaagaagat tttaatatat
    34321 atgtgaaatt aaatgataaa agtaatttat cgatagaaca agcaaaatca aaattaaata
    34381 agattgctaa tgatgtagga ttggacacta ttaatgtagc tgtaaatgaa aactatataa
    34441 atgctttaaa accagatatg gaaacaatta tgggtggagt atttgtaggg ttagtaattg
    34501 tattgtctag tattttagta atatacaata tattttatat ttcaatagtt acaaaggttc
    34561 aagaatttgg aaagcttaga gcaattggtg caactaaaaa acaagtaaaa aatatagtat
    34621 ttaaggaagg gttcatatta gccggaatag caataccaat aggaataatt ttaggctatg
    34681 tacttgcaaa tattataata aaatcattta tggatataga tgtaaaatca tcacgattac
    34741 cagttatatt atcggttgtg gtaataagct ttatatctgt tgttttatca cttttaaaac
    34801 caatgaaagt agcatcaaaa gtatccattg ttgacgcagt aagatataca ggaaataaaa
    34861 taagtaataa gagtaagaga aaaggatata aaaatataaa tctaaagaga ttatcacatg
    34921 ccaatttaga aagaaataaa aaacgtactt acatgacatt ggcatcactt atacttagtg
    34981 gaactatatt tataacagta tcaactgcct tagaaagttt tgatgctgag aaaatggcaa
    35041 gagaacactt tccttatgat atagaagtta gactatctgg ttatgaaatg aacagtgata
    35101 aaaatcctaa agataatttg aatattttac aaatggataa tcctctaagt aaagattttt
    35161 ttaatcaaat aaagaacatg gaaggaatta aaagaataga atctgcaaga agtgtaaaaa
    35221 ttggtatgga ggactatgat gttgaattta agtatgacct tttacaaagt ataaatgaaa
    35281 atgatgtaaa atcattaaac aagaatataa tagatggaaa aataaatctt gaaagacttc
    35341 aaactggaga tgaaatagta attactcatg ttgatacagc aaaagaaatg ggagtaaaag
    35401 caggagataa aataagatta actttatatg atggagataa aaagataaaa aaagaattta
    35461 aagttcaagc aatagctatg ggggttccaa gttttggtat agggaaggat tttattgata
    35521 gaactttgaa atacgattca actagtagct tgggaatata tacagaagaa ggaaagtatc
    35581 aagaaatcaa agatagtatc aagaagattg ctaaaaataa tggattttta gagactgatt
    35641 ttatagatag tagaattgag tcaaataagg ctactatttc atttataaag ataatgggat
    35701 acacgctaac aggcataata ggtgtgattg gatttatgaa tcttgtcaac acaatgataa
    35761 ctagcatagt tactagaaaa aaagaacttg gtatgcttca agctattggt cttactaata
    35821 aacaattagt gaaaatgctt aattcagagg caatgtatta cacttctgga atgatgatag
    35881 gaagtattct atttggaggt atattagggt atattgctgt tatatttcta aaaaagactg
    35941 gattatctta tgcaacatat tctttgccaa tagtaccaat cttgttgatg attgtgtgta
    36001 ttcttatagc tcaatttata acaacttatt tgataggacg aagttttaat aaagaatctc
    36061 ttattgatag agtaagatac agtgaataaa atacaaatat ttatggttag tttaactagc
    36121 aaaaaacata taaaaaaata tagttaaaga gattcataat taaataggtt aaagcatagt
    36181 acagagcaaa caatttaagc agatgtttgc tctgtatttt attatgtaat caataatata
    36241 tctaatatac tgatattatt catgttagaa aatgtaccgg tacaattcta aaaaagtata
    36301 aaattgtacc atataaaatg aaaattttgt ataataatcg tatagaaaat aaataggagc
    36361 tgatatatat gaataagaag attaattatg caccaggacc aactgaaact agggaaaatg
    36421 taagattagc aagagcagaa aaaacaacaa atccagatat agatattgat tttgttgaat
    36481 tttataaaaa gacatgcgag aagtttggta atataattgg aacaaaaaac gatgtttaca
    36541 tattgagtgg tgaaggaata ttaggtcttg aagcagcatg tgcatcactt acagaaaaag
    36601 gagatagagt acttgtaatt gacaatggta tatatggaga aggttttaaa gattttgtaa
    36661 caatgtatgg tggggagtat gtgctttttt caagtgaata tactaaaagt atagatattg
    36721 atgaattgaa agcattttta gatgaggata gtaattttaa atatgcaaca gtagtgcatt
    36781 gtgatactcc aactggcgta ttaaatgatg taagcaaaat atgtccatta cttaaagaat
    36841 atggaatatt aactgtagtt gattctgtag caggtatggt aggagaaaga ttaagtgtag
    36901 atgaatctaa aatagatata atattaggtg gttcacaaaa agctatttct gcaccagcag
    36961 gacttactat agtcggaata agtcaagatg caaaaaattg tataaaaaat agaaaaactg
    37021 atgtaatagg tttttattgt aatttaaata tatgggaagg ctattatgaa aaaaaatact
    37081 tcccttatac aatgccaata agtgacataa tggggcttga taaagcactt gataatatat
    37141 tagaagaagg aatagaaaat gtattaaata gacatgaaaa gatagcttat agtgttagaa
    37201 aatctattga ggaatatggt ctagaactat ttttagaaga aggatattct aatacagtca
    37261 cagctataaa aattcctgaa agtataggag ctttagaact tactgattat atgcttaaaa
    37321 attataatac acttgttgca acatcactaa atcaatatat ggatactatt ttaagaatag
    37381 gtcatatggg agagaatgca aatctaaata aaatagaaca tgtactaaat gttttggata
    37441 aaagtttaag tgcattagga ttcaaaggaa atggaaattt gttaaattta tttaataagt
    37501 attattttta atagataggg ggatatagta tggaagttac agctaagcaa aaatttacaa
    37561 cgaaagacat ggtggaaacc tcactattaa tagcattagt atttattgca actaaattta
    37621 taaacataag attaccaata tctataaatg gtgggcttgt gcatttagga actgcaatgt
    37681 tgttcatatc agcaatagta tttggaagta aaaaaggtgc tttatcagga gctattggta
    37741 tgtcactttt tgatttgata tctgggtgga ctttatgggc accgtttacc tttatagtta
    37801 gaggagttat gggttattta cttggaaaga ttgcatgggc aaatggtaat aatgggaata
    37861 attttttaat caatgtaata ggaatatgtg tttcatcaat ttggatgcta tttggatatt
    37921 atgtaacaga agttatactt tatggtaatt ttatagtacc tcttacttca ataccaggta
    37981 atttaatgca agtattgata ggtcttataa tagcgcttcc tatttctaag gtattgaaaa
    38041 aatgtataaa ataaatataa agtgaactaa aatttatgat agcataaatt atactgtata
    38101 atattaggat ggagtacata attaaaaaat tttagtatag aggttagttc acaatgttat
    38161 cagttacaag atattcaatc aaaaatgttc gtttaaagcc gtgggtaaag tttatttggt
    38221 attttgagac aaaatcaaat atactgttaa ataataagtt acttccgaca gacagtatag
    38281 atattattct caatctttct gatgttatgg aatataaaat tgaaaatcaa gactatattg
    38341 ctagtaatat tcattttaat ggaataaggg ataaacatgg atttattatt caacatggaa
    38401 atatacgtgt tatagggatt tctttttatc catttggtct ctatccattt ttaaagatac
    38461 caatttcaga gtttaacaga caaattgttg acctagaagc agtatcaaaa ctatttgcaa
    38521 aaaaattaga agagtcatta aatccaacaa aagcaataga aaaagttgtt ttatgtttag
    38581 aagaagtatt attatccata ttagaagaag atttaatttc aaataaatat gtaaagcttc
    38641 taaattcatt tatatatgtt aatagatata gcaatataaa agcattttgt gatgatacaa
    38701 atataaatat taaaacattg gaaagaatct gtttaaaata tacagggtat acacctaaga
    38761 ttttaaagag aatttacaga tttaaaatgg caagcaatca acttatttat aattataaag
    38821 atgatgaact ttttgatttt atttatgaaa atgagtacta tgaccaagca cactttataa
    38881 aagaatttaa aaaattttca gggacgtcac caattaaatt tagtggtgag aataagacga
    38941 ttaaagaaaa tataaagtac agctatttat aattatactg tcgatttttt acaatacctc
    39001 caatttattt tttgatatga tataggaaaa taaattggag gttttacata tgaaaattat
    39061 tacagaaata gttgagttta atgtggaaga aaatttatca aaagaagagt ttatagaaat
    39121 tgtaaatgac ttagaattaa aatttcattc tattcaatct ggttttattg atactgagct
    39181 tttatatgat gaaaagtctc aaaagtggat tatgatacaa cattgggaat ctatagaaga
    39241 cctcaagagt gtttctagta aaatgtttaa agattcttca actgagaaat ttagaaaagc
    39301 tttaaagaat caaactgtta agatgaatat aataccacaa attaaaagtt ggaaattata
    39361 gaactgaatg taaaagatag ttaaatagta tcaaactaaa ttagccaaat aacattttgt
    39421 atttaaattg ttatttggct tttagtgttt attttaatat gagtgttata tactagaaaa
    39481 accaacaaac tgttagagtg tggattatca taatgtcata ttcgggagct aaaaggctta
    39541 atactgattt gccaattggt caatccatgc atgtccatct ggatatgctg ttattaaagc
    39601 atttacaagc caatcccttt gctcttgtga taatagtggg actgttacat cgaagtcttt
    39661 ttgataacca agcctatcta tgtatattga tttgcaaaac aaaaccactt cgggagccat
    39721 atatgggata ttatctgatg ttatcagtct ggccttatcc atatctctta taattctttg
    39781 attttgggaa aaaagaaatt ttccatcttt ctgtggatta tatatcaatt caataaaatc
    39841 gaactgtaac tgttcgtcac actgtatatt cattttatag ttatcatcct cgactttttt
    39901 taattcaata aatgagcaat caggcttaat cgcaaaaaca caaatttgct ctatatgttg
    39961 cttgttaagg tcgataatag gtcgtaataa ataattaccc aagggctcat atatatccca
    40021 tccttgatta agcatatact gaatgctctt ttctttatct gtgtcagaaa tccatatgtc
    40081 aatatcactg tgtgggcgaa tatgcatatt cgcaaataat tctagggcat atccaccaca
    40141 tattgaatat gaaaagccac aatcattaaa aaagtttaca caatctttaa ttagtttgtt
    40201 catagcaacc tccttttgca tgtactgaaa taaattaaat tttttaaaat accaactaaa
    40261 tgtttgtttt gtgtaaatat aaaactgccg atattaaggc agttttatat ttactattta
    40321 gaaaatttat taacaaattt ttgatattca cacattatct tatcattcat aaaagaaata
    40381 cccataaaag cagcttgctc accaaacaaa gtaaaacgtt ctatcatctg ttgtggatta
    40441 cctggataaa ctgttggaat acaccatgtg tttagaagaa aaccaaacat ttgagcagca
    40501 atctctggat ttttaacttc catagaatta tcttgatttc cttcttcaat caatgctttt
    40561 atccaaggca caatatcatt tataatctca tcaaactgcg tagctaaata cttaggatta
    40621 cgcaatagtg atgatgacat taaaaaaatg tcttgtttat tgtgctgttc ataagtatga
    40681 agcaacctat attgtaattt ttctaatgcg gtctttcctt catcattttt tacctcatac
    40741 ccataaaatc catcattata caaccgactt gtgattgcct taattatttc gtcttttgat
    40801 ttaaagtgat gataaatagc acctcttgaa aggccatcta aattatctac aatgtcttga
    40861 atcgttgtct gctcataccc tttttctaag aataacttat gtgaaatatc aagtattttt
    40921 tgtatagtat tatcaggttc tttttttctt cccaaaaaac tcactcctcg aaacaatcat
    40981 ttggtatgtt tatcttagca aatacataat gatttgtcaa tgtgtctttt tagcatgttg
    41041 aacaggtata ttgccatgct ttcattattt tatttttttg ccttatactg gactatattt
    41101 gcagtttcct aatatatttg gctcatattt aaataccatt atctaaaagc actattgctg
    41161 tacgagaggc aataggcatt ttagaagaag gaggagttga gctaaagtat acatcataat
    41221 ctcttaagtt ttcttttttt gtgtttcctc caaactctac catatcagta tttaatatcg
    41281 ttttgtagtt tgtattttga aactttgaaa tatcaactga ttgtattaga ttagggtgga
    41341 aattaaatat aaataaatat ttgcccttac tgtacaccaa aatcttttta tcattatcca
    41401 tatgtataag ttcatgacta ttttggataa aaatatcact gtattttgta agttcgagca
    41461 tagatttatc aaaatttaat aattgtttat actttaagtt gttatcttct gaaaggctcc
    41521 attgtcttct ggcatactta tagctccaat tgttgccttc tcgtggaaaa tcaatccact
    41581 caggatgtcc aaattcattt cccataaaat ttagatatcc ttcacctgct agagaaaaag
    41641 ttattaattt aattaattta ataagagata ttgctctatt gataacatgg ttatttgagt
    41701 ttatttccat attccagtac atttccttgt cagctagcca aaaaataatt gttttatcgc
    41761 caactaatgc ttggtcatgg gattcacaat atcctatatt tttttctcca ggtcgtctag
    41821 tagtaagttc ataccacatt ttgcccaaat cccagtcttc atcagataag ttagagatag
    41881 tttttatcca aaaatcaggt actcccattg cgagtctata gtcaaatcca attccgccgt
    41941 cttttatagg gatacacata ccaggcattc cactcatatc ttctgcaata cttatcgaat
    42001 taggtttaat ttctttcatt aattcatttg caaattggag atatgttata gcttcaatat
    42061 ctgtattcat actaaaatat ttttcataac tatcaaatga aactccaaga ccatggttat
    42121 gatataacat tgaagttaca ccatcaaacc taaagccatc aaaatggtac tcatttaacc
    42181 aaaattttat atttgaaagc aaaaaatgta ttacttcagg tttaccatag ttaaaaagtt
    42241 ttgtacccca agcaggatga tttccctttg aacctgagtg gaaaaattga tgttcacttc
    42301 catcaaactc atttattcct tctaaagtat tctttacagc atgagaatga actaaatcta
    42361 ataaaacagc aatccccatt gaatgagcag tgtttatgag gttttttaag tcttcaggag
    42421 taccaaacct agatgagatt gcataaaagt ttgaaacttg gtatccaaaa gatgcataat
    42481 atggatgttc cataatagcc ataagctgaa tggtattgta tccagctttt tttatcttag
    42541 gaagtatttt ttcagtaaat tcattataag ttccaataga ttcgctttca gtagacatac
    42601 ctatatgaca ttcatagatt agtggagaag ttatgttttt taagtcaaaa tcattgtcag
    42661 tccagataaa tggagttttt ggttgccata tttgtccatt aaatccacca aaatcttttt
    42721 gaattactct ttttatatat aatggtatac ggtcaaatgt ttttccattt gcagtaactt
    42781 gtacctttac ctctgatttg tgaggtaagc tattttgacc aggaataaat atttcccaat
    42841 taccagaccc aatttttctt agaggatgag attttctatt ccaattatta aagtcaccaa
    42901 ttagtgacaa actatctgca tttggagccc attccctata gaagaatcca tcatttgtct
    42961 gatgaaatcc atagtaaaga tgagcatttg caaaagaact aaaatcttta tagtttttca
    43021 atgtttttga tttcatatcc tcataatgtt tcattcgctt ttttatatca gattcaaatg
    43081 gttgtaggta tacatcaata tctaaaattt taaatctttt ttgacttttc attgtatttc
    43141 tcctccataa taaatattac atctaaaaaa taatatttat ctcatactat cttctctgta
    43201 taattgatat atattatata atcctaataa aaagcttaag ccaccaatga ttaatttcat
    43261 aatttacctc cctagtattt atacttatat atcgtattca tcaaaattta aaagtttgtt
    43321 taaaactctt ttaataatat cataatcaaa gcctttatag gaaagatgtt gagaaatttt
    43381 ttgatatatt ttctttttat cttctttctt tactctttca tacctttttt ttgctagata
    43441 catagcattt tcaaattcag tgtccttgtc taattcagat actgcttcat tgattgtact
    43501 tctattgata cctttattat ataaattctg cttaattctg tttttgccac atttatttag
    43561 gttaacattt gtgttaacaa ttttttgagc taatacactg tcatttacga gattataact
    43621 tttaagaaaa tctatgaccc tctcaattat attttcatca aattctgatg atagtttttc
    43681 ttttaatttt ttttcagatt ggtcagcatg agataaaata tttaatgctt tatttttagc
    43741 ttttatatac atttcatcat ctaaaatagg ttttaattct tcttcattta tttcattccc
    43801 tttttttaaa ttaaatgtat aaaccagttc tttaaatata gcaataaaaa atttatcatc
    43861 tacataaata ttaactctat catcatttct tttttgttgt tctatttttg taattatacc
    43921 cataagtcct ccttttttat atg
//
LOCUS       DAMJZE010000016        41933 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJZE010000016 DAMJZE010000000
VERSION     DAMJZE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.016"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(75..2030)
                     /locus_tag="PC541_000765"
     CDS             complement(75..2030)
                     /locus_tag="PC541_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838239.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(2213..3688)
                     /gene="spoIVA"
                     /locus_tag="PC541_000766"
     CDS             complement(2213..3688)
                     /gene="spoIVA"
                     /locus_tag="PC541_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2838240.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3860..5155)
                     /locus_tag="PC541_000767"
     CDS             complement(3860..5155)
                     /locus_tag="PC541_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2838241.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5339..6748)
                     /locus_tag="PC541_000768"
     CDS             complement(5339..6748)
                     /locus_tag="PC541_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2838242.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(6800..7939)
                     /locus_tag="PC541_000769"
     CDS             complement(6800..7939)
                     /locus_tag="PC541_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2838243.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8258..8908
                     /locus_tag="PC541_000770"
     CDS             8258..8908
                     /locus_tag="PC541_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2838244.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(9065..10084)
                     /locus_tag="PC541_000771"
     CDS             complement(9065..10084)
                     /locus_tag="PC541_000771"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2838245.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(10115..10729)
                     /gene="plsY"
                     /locus_tag="PC541_000772"
     CDS             complement(10115..10729)
                     /gene="plsY"
                     /locus_tag="PC541_000772"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2838246.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10764..12083)
                     /gene="der"
                     /locus_tag="PC541_000773"
     CDS             complement(10764..12083)
                     /gene="der"
                     /locus_tag="PC541_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2838247.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(12117..13445)
                     /locus_tag="PC541_000774"
     CDS             complement(12117..13445)
                     /locus_tag="PC541_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2838248.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13683..13883)
                     /locus_tag="PC541_000775"
     CDS             complement(13683..13883)
                     /locus_tag="PC541_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838249.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13885..14817)
                     /locus_tag="PC541_000776"
     CDS             complement(13885..14817)
                     /locus_tag="PC541_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2838250.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14862..15794)
                     /locus_tag="PC541_000777"
     CDS             complement(14862..15794)
                     /locus_tag="PC541_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2838251.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15849..17138)
                     /locus_tag="PC541_000778"
     CDS             complement(15849..17138)
                     /locus_tag="PC541_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2838252.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(17167..17859)
                     /locus_tag="PC541_000779"
     CDS             complement(17167..17859)
                     /locus_tag="PC541_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838253.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17874..18635)
                     /gene="pgeF"
                     /locus_tag="PC541_000780"
     CDS             complement(17874..18635)
                     /gene="pgeF"
                     /locus_tag="PC541_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2838254.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18632..19093)
                     /gene="nrdR"
                     /locus_tag="PC541_000781"
     CDS             complement(18632..19093)
                     /gene="nrdR"
                     /locus_tag="PC541_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2838255.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(19115..19378)
                     /locus_tag="PC541_000782"
     CDS             complement(19115..19378)
                     /locus_tag="PC541_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2838256.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19482..20255)
                     /gene="sigG"
                     /locus_tag="PC541_000783"
     CDS             complement(19482..20255)
                     /gene="sigG"
                     /locus_tag="PC541_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2838257.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20347..21069)
                     /gene="sigE"
                     /locus_tag="PC541_000784"
     CDS             complement(20347..21069)
                     /gene="sigE"
                     /locus_tag="PC541_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2838258.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(21094..21966)
                     /locus_tag="PC541_000785"
     CDS             complement(21094..21966)
                     /locus_tag="PC541_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2838259.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(22256..23530)
                     /locus_tag="PC541_000786"
     CDS             complement(22256..23530)
                     /locus_tag="PC541_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2838260.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23814..24971)
                     /gene="ftsZ"
                     /locus_tag="PC541_000787"
     CDS             complement(23814..24971)
                     /gene="ftsZ"
                     /locus_tag="PC541_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2838261.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(25161..25514)
                     /locus_tag="PC541_000788"
     CDS             complement(25161..25514)
                     /locus_tag="PC541_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2838262.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25613..26287)
                     /locus_tag="PC541_000789"
     CDS             complement(25613..26287)
                     /locus_tag="PC541_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2838263.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(26292..26990)
                     /locus_tag="PC541_000790"
     CDS             complement(26292..26990)
                     /locus_tag="PC541_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2838264.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(27013..27753)
                     /locus_tag="PC541_000791"
     CDS             complement(27013..27753)
                     /locus_tag="PC541_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2838265.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(28023..29249)
                     /gene="murG"
                     /locus_tag="PC541_000792"
     CDS             complement(28023..29249)
                     /gene="murG"
                     /locus_tag="PC541_000792"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2838266.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(29267..30397)
                     /gene="spoVE"
                     /locus_tag="PC541_000793"
     CDS             complement(29267..30397)
                     /gene="spoVE"
                     /locus_tag="PC541_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2838267.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(30449..31804)
                     /locus_tag="PC541_000794"
     CDS             complement(30449..31804)
                     /locus_tag="PC541_000794"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2838268.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(31823..32791)
                     /locus_tag="PC541_000795"
     CDS             complement(31823..32791)
                     /locus_tag="PC541_000795"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2838269.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(32832..34205)
                     /locus_tag="PC541_000796"
     CDS             complement(32832..34205)
                     /locus_tag="PC541_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2838270.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(34372..36351)
                     /locus_tag="PC541_000797"
     CDS             complement(34372..36351)
                     /locus_tag="PC541_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2838271.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(36363..36710)
                     /locus_tag="PC541_000798"
     CDS             complement(36363..36710)
                     /locus_tag="PC541_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838272.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(37191..38126)
                     /gene="rsmH"
                     /locus_tag="PC541_000799"
     CDS             complement(37191..38126)
                     /gene="rsmH"
                     /locus_tag="PC541_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2838273.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(38156..38902)
                     /locus_tag="PC541_000800"
     CDS             complement(38156..38902)
                     /locus_tag="PC541_000800"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2838274.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(39296..40582)
                     /locus_tag="PC541_000801"
     CDS             complement(39296..40582)
                     /locus_tag="PC541_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2838275.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(40597..41862)
                     /locus_tag="PC541_000802"
     CDS             complement(40597..41862)
                     /locus_tag="PC541_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2838276.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        12971 a         7816 c         4303 g        16843 t
ORIGIN      
        1 aaatacagtt ttcataataa tgaaaggtat tacttagcaa tcgcatgatt ttctaaataa
       61 tacctttcta aaaattattt ttttaatttt atgaatttaa ttatactttt tctagtatca
      121 ggatttacaa ttaatataga accaatcata attggtgtta gtatacaaaa taaaaaaata
      181 atatcttttt ttagattata ttctttagat tctaatttat aattattatt tattattcca
      241 tcataatcac ttatatttga cttattttta tttgtatctt gatttttgtt agaaatactt
      301 tccctataag gaaaagaatg ttcattggta ccaaatacta attttgagct taaatcataa
      361 ttatcatcat tttctgtaat tgaattttta ttgtaaaatc ctttttcttt atcagaatta
      421 ttttctttat taccgtcttt aggtatatca tgaggatttt tattttcatt gctatcagta
      481 cttgattcta catcatcttt aggagtattt ggttgtttct catcagaatt atcattttca
      541 ttattattat tttcattatt attgttttga ttgttattac tatcattatt ttcatttcca
      601 ttattcccat cttgattgtt atcatcctca tctctatccg ggtcgtctct acctcctcct
      661 ccacggtcac catcaatatc atcattttcc cgttcatcat ctggtgtctc ttggttgtct
      721 ccgcctccca ttataaatcc tggtttaaaa actatatttg aatacccttc ttttaatcct
      781 cctaccactt ttactctaaa acaacgcaac ttaaaatctt caaaaaaaag tatagcttga
      841 tctctaatat cctcctgttc ttcagatatc caattgattc catcgcttga atattcaaca
      901 aaaaccttac ttgcactata cggcatatca taaaataaaa cagctacata tccatttttt
      961 gtattttgaa attccagttc caaattgtct attggttttt tctctataac agatacattc
     1021 aaaataggaa ctacatcatc atttattata attttttctt tatttaacat ttctgtttta
     1081 aactttcctg ttatattaaa attttctccc ttttccaact tttcataata atcagtaaaa
     1141 tcccattcta catctatatc aagtcgatta ccactagatg tttctaatac catatatttg
     1201 ggaaatttta tattttctat tttatcatct ttacttactt ttatttcatc actaaataca
     1261 ccaattcccc tatgacaaca tttcacaata tcatagccag aatcttcagc tatagagggt
     1321 ataagctcat tataatcatc aattatataa actttcttac tttctgatat aattgattgt
     1381 gccaacttat tattatcttt tagtaaccca ctttcattgt tgctactata tgcttttatt
     1441 gaagtttctt ctatctcaac atcaccttta gaataaacac ctatagcatg ttctccattc
     1501 acttcaatat ccttatttag tattctaata tcattttcag tataaattcc tattgcatct
     1561 ttcccatttg cctttatttt ttcaacagat tcgtaggatg atttaatatg taagtctcct
     1621 ccttcttcaa cgtataaggc tgtcccatca gtagcaatta tgttgtttat accaatactt
     1681 atacttccac cattttctat atgtattact ccttcttttc ctccttcacc tattatattc
     1741 aattcattat catcaataat aaatctccca tgatttttta tcaatatttt atatttgttg
     1801 gtatctattg taaaattact gtcataagga atataaaatc taaactcttc atctattacc
     1861 atatcacaag aaagagtata aactatttct tctttattat ttttattttc ttgaacccac
     1921 ctttcaaaat cttcaagaca attttcctct tgtccaaaaa ctttatgtgg aaaacttata
     1981 aaacacaaaa ctattatact cataaaaata aatatctcat actttttcat cttacactcc
     2041 tagaaatttt ttagctttga aacaatctgt cgaaacatac acttcaccca ctataatttt
     2101 tccacaatta tcaattattg tcaatttata aaagtatctt tattaattgt tataaataaa
     2161 aagctactta ctaaagtaaa cagacgtttc actttaataa gtagctatat ttttataaca
     2221 aaatagttat aatattagaa cttccttcat ttacaatttt ttgtaaagtc ttttgcattt
     2281 taactcttat ctcttctggc atagtatata atttactttg taattgctct tttactaagt
     2341 catgtagtga tttaccaaac atatttgatt cccataaatc tgctggttgc tcttcaaata
     2401 cttccataag atattttatc atctcttcac cttgattttg atttccaact attggagaaa
     2461 cctctgtaga tatatctgct tttataatat gaagtgatgg tgcattggct tttaacttga
     2521 taccatattg ttttccttgt ttcattattt ctggttcttc cagactaagt tcttctaagg
     2581 aaggagctac tactccatat ccttttatct tggcatctat taaagcactt tctattttat
     2641 catactcatt tttaacttta gatagtctag ttatcagact aagcagttga taatctcctt
     2701 caattttaaa tccacttttt tcttcaagga cattatagaa taaatcttgc tttgtctgta
     2761 aatcaatatt tataacacct tctcctagct ctacattatc aacacctgta tcttccaaaa
     2821 actctaactc agaaaatcct tgtataatac cttctatatc ccttatttta cctatatcta
     2881 taatactttg ttttaatgtt gttattatgc tactttttat ccaatgattt ctttcaagac
     2941 cttcaaccca ttttggtaag ttgattctta tttctgtaag tggaaaatca tataatacag
     3001 cttccataac ctcctctata tcttcttctt ccatttcaac cacattcatt ggaagaactg
     3061 gaacttcata cttttcttcc aattcacttc taagcatact cgtttcctca ctttttggac
     3121 ttaacgtatt taaaaccact gcaaatggtt ttttaagatt tttcaattct tgtataactc
     3181 tctcttctgg ttctacatag ctttttctgt caattccagt aactgaacca tctgtcaaga
     3241 caactatccc tattgtagaa tggtctttta taactttttt agttcctatc tctgctgcct
     3301 tttcaaatgt catagcttct tgtgaccaag gagtggatac taatctttgt tttccacctt
     3361 cctcatgccc taaagctcct tcaactatgt atccaacaca atctaccatt cttactttta
     3421 aagatactgt gtcttttatt tttatttcta caccatctgc tggtacaaat tttggttcta
     3481 ctgtcattat cgttttacct gaaccacttt ggggtatctc atctcttgtt ctatcctttt
     3541 taaattcatt gtctatatta ggtataacca acttttccat aaattttctt ataaaagttg
     3601 attttcctgt tcttacaggt ccaactactc caatatatat atccccctga gtccttttgg
     3661 atatatcttc gtatatgtta ttattcatat tattcctcct atacatctaa tcttattaaa
     3721 ttatatttag agttaagggt taatattctt aaaatagaaa aataaagacc agcaatatta
     3781 atttaattta tatatagaaa tttgtaaatt tcaatgtaaa ttaaattaat attgctggtc
     3841 caaatagtag tgtattatat taactgactt tagtagtatt ttcatttgaa ttattattca
     3901 tctcaataag ttctttacgt ctttcttttg tcatgttcat ccatataaat gtaaacacca
     3961 atccaattat ggctactata gtaagaacta tgaagtagta cctatatcca gttacacccg
     4021 gaaatgcatc caatatcttc cctgctgcta aaggacaaag tacttctgga agatatccta
     4081 acgtagcaac tattcctata gcagttcctg atattgataa tgaataatta ccttcttcaa
     4141 gtaacgaata gtgtaaacct tgaattgcat acatagatac atatattgca acacatccaa
     4201 tcaatagtat ataaatcata gatgattttg ttggtgtaaa tattacacct aaaagacctg
     4261 caatcataag tataaatcca taagatataa tctttgatga accaacttta tctccaagta
     4321 atccagaagc agctgatgct actggtctac aatactgagc taatattgag attgcagatg
     4381 caaacaccac agtagctcca aatgcttcag ttgaataagg cgtaaaataa taaaatgaca
     4441 tattagctga atatgaacaa aacattatta ttgttattat ccatgtatga ggcattttta
     4501 ttactgcaaa aaaggcttct ttatcaaatt ttgctgatac ctctttttca ttatccttta
     4561 atttgaaaaa tactaatact ccaactatta catttattgc tgagtataat actataatag
     4621 ctgtaagacc agattcatca cttacttttg atgaaatata cccaaatagt atcaatacta
     4681 gagatagatg aattgcattg gtaattccac gaccactttc cataaatcca aaagcttttc
     4741 cttgttcatt ttcagatgct aacattctaa cagcttttac aagagctggc caaaaagtaa
     4801 gtattgttgt tattccccat aaggcatgga ttaaaaacac aacaatatat ggaggataaa
     4861 gtgttaatac aagaccagat attcctgtaa taattaaaga acctgaaagc aattttctgg
     4921 ctgaaatacg gtctgctaaa aaaccaccaa aaaggtatga taccattgca agtagaccat
     4981 aaatgcttcc taagcttccc atttgtgtat ttgtaagatt aaatgcttgt gcaaaagtat
     5041 cataataata attctttaaa tatggtaaag catatataaa tgctcctgct acaaagagta
     5101 ttatcaagac tttgaaattt ttccaaatgc ttggtttatt tacttcaact gacatacttc
     5161 ctccttatgt aaatacctgt aaattttcat aataattaat tattgcttat tttattgttt
     5221 attcaatatt taggaatatt tattaatata ttaggttaat ctatcaataa tttatggtga
     5281 gttatgagct aaaagtaaac tcttttagct caccataata attatatttt tatttatact
     5341 aacatccaga gttattagat atcttttttt catagttctt ccatagcatt aaacctgtta
     5401 ttataacaaa aagacctcct cctatcattt caatctcata gattatatta tcttttaaag
     5461 tctccataac tggtgccgtt gagaaaagta tgccaactac agttactaca agtaccattc
     5521 tggcaatatt gatacaaact gaatcacttt ttgcaattga atatggtctt tcttcatttg
     5581 gtcttgtctt tctaagtcta atataagatg taaaaagcaa gacatatgga aataatgctg
     5641 ttaatgctgt catagtaaca agaacattgt atatattatc tactgatggt aataagtttg
     5701 ttcctacaag aagtagactc actattatag cctgaactat tactgcattt gatggtatat
     5761 tgtatttatt tgtaacagtc aattgcttag gaaatacacc ttctcttaca tttccaaaaa
     5821 gcatctttac tggacctgct atataaagta ttaatgctcc tagtactgat aaagttattc
     5881 caaatgctac tattcttata aaccaacttc ctatacctaa atcttgtgca actttagcta
     5941 atgcatctag tatccctgta gatgctgcaa ttttatctgg aggaagtatc attgttattg
     6001 ctactgaacc taagatatag attcctccta ttaaaacagc cacagttaat atagctttag
     6061 gaaagttttt cttagcatta tccatttcgg ttatgaaatt agcagtcgtt tcagctcctg
     6121 ataatgcaaa tattatagca gatatagaaa ctaatgaatt ggcatctatt tttggtatta
     6181 tattttggat tgtatatatc gatgctgatg gagctttttt gagaataact acagacataa
     6241 atgccatcac tataagtaat atagcaggaa ttgtagaacc taatgctcct gtattcgtaa
     6301 aaaattttcc aaatgccatc ccacgagtac ttactaagga caacagccaa aatattatga
     6361 gtgaaagtgc taaaataaac atcttattgt ttgataaatc tggatttcca agcatatatg
     6421 caatatttat cgctaaaaaa gtcaagaaag aagaatacca aaatatcttt gatgtccaat
     6481 ttagccatga caccataaat ccccacttct ctccataagc ttgtttaacc cattctccaa
     6541 gtccaccatc ttttggatat gttgcagcca actctgcaca tataagtgat gcaggaacaa
     6601 agaatataaa agcaaacaat aaccaaactg gtatagaacc aagacccaat ccaaaactag
     6661 aggcagtaga tttagctatc catctaatcc caaatagtgc tgatatattc ataaatacta
     6721 aatcccaaaa tccaattttc tttttcactg taattcctcc aatttcaaat ttattcagac
     6781 tcatacacaa tgtattgact taaatatcaa gtataagttc ttcaacactt cttggataat
     6841 tagttataat tgtagcacca ttcttaccta ctattgctgt atctgagtgt ctaaatcctc
     6901 caactccagg tatatatatc cctggctcca tagaaaatac cattccttct tctaatatta
     6961 attcattgtc aaatcttaaa tatggctctt catgttctga taaacctaaa ccatgaccaa
     7021 ttctatgatt tgaatataac tctagaccat actttgcaac aacagctctt gctgcttcat
     7081 ccaccattct tgctggtata ccagctttta ttgtatctaa tccagcttgt tgagcctcta
     7141 ctgcaatctt aaacacttct ttttgtcttt ctgttggttt gcctataata aaagttcttt
     7201 cattttctgc tctatagttt tcataccaaa cctgtctact atgtattact atatctcctc
     7261 tttgaagtat tctagtattg gaatctaaat gaggttgtgc tgttctatca atccctgaac
     7321 aagtccagtt tgcaaatcct atatatgtat caggataatt ttcagaaaca tattttagta
     7381 aagcattatc ccctgcaacg tcaaactcca attcactcat cccaactcta acattttcta
     7441 aagacccttt tattcctata tcagataggt aacctgctat cttcaaaaat tctatttctt
     7501 ttgcatcttt tacatatctc atttcaaata ttttacttcc aacatctttg atagcaaatg
     7561 acttatcagt tatatactct gaaaagcttg cagatactat atctttttct attccaacag
     7621 ttgttccttt agggtattta tttaaaatta tatctaagta atgtttatgt gatattccat
     7681 gttctttcat ttctggtatt tcataataca catatacatt atcaactttt gcaacttctt
     7741 tagcatgtaa ttcttctaat ccaggtacta ttaattctat tttatcttga gttactactg
     7801 ttacaattgg tctagaataa gttattgctt taaatccact aatataaaat tgattgtctg
     7861 gttcaaatat taaagcaatt tcaatgcttt tttcattcat gtactttctc aatctttcta
     7921 gtctcatatt atttgacata atgcacctct ttcttaatat tcagataatt tattcaatac
     7981 aacaaggatg aatatattat tttaaaattc ttaatatttt tagtacttgt cgtatttgta
     8041 tgtaaattaa ttttatttgt gattatttta tcataatcat gattattttg tcaatcatga
     8101 ttttatttta aaaaattttt tatattctag tgattttaaa ttatctaaaa tataacgagt
     8161 atacattgta ttttgtaata ttattcttga tttatggtaa gtttaatgat aaaatcacga
     8221 ttatataaac tatattgatt atttgtaggt gaataaaatg acaaataaag aattacaaaa
     8281 gaaaaggata ttgatgtatt tcatagaagc tgctcaaaat attatggaaa acgaaggtat
     8341 agaaaatatt acacttcgta aggttgcaga tatggctggc tataatagtt caactctata
     8401 taaatacttt aaaaatttag accatttaat atcatttgca tctataaaat attttaaaga
     8461 ttataacttg aatatttcaa gatgtataga aaatgtcaat gatgaatata agaaatatat
     8521 aattatgtgg aaattatttt gtaagcattc ttttgataat gctcaagctt tttaccaggt
     8581 attttttaac ttgtctagtg atgaacttag ctatataaca aaacaatatt atgatatgtt
     8641 tccagaagat ttaggtattc atgatagtga catatcttta atgattacag gagaaagtat
     8701 aaaggaaaga aataagatac tattaaacaa tcttgtagat actggatata taccagctaa
     8761 agatttagac ataatcaatg atataattat ttctttttat caaaatctat tattcttaaa
     8821 aaagaacaca ggaaaaaatt ctgatgatga tgaattaact atgaaaatga tttcttctat
     8881 tgaatacata ataaaacatg ctaaataaaa ttaaaatatg gctgtctcaa aatagagata
     8941 agcctttttt attgttgaaa taataaaaat atatttaatt tcatataaaa aagagtgcct
     9001 cgtaaactaa aaagttcatt ttgagacacc cctaaaataa atttattatt tattatacaa
     9061 tagtttatat atcatcacct aaaactactt cttccatttc atgcttttta cttctcatca
     9121 ttagttctat tccaacttct ttaggatttg aacctttctt tattacagaa tatatagcat
     9181 tagttatagg catatctata tctaattttt cagcaacatc atgtgctact tcagtagcag
     9241 ttatgccttc tacaaccatc tttacttctt ttaaagtatc ttctaaactc atccctttac
     9301 ctatgagtat accagctctt ctatttctac tatgcatact tgtacaagta actattaaat
     9361 ctccaatacc tgaaagacct gcaaaggtag acatattagc tcccatagca attccaagtc
     9421 tgctcatttc actaatacct cttgtcataa gggcagcttt tgcattatca ccatacccta
     9481 atccgtcaca tatcccagct ccaaatgcta taatattttt taatgctcct ccaagttcta
     9541 ctccaactat gtctggattc gtataaactc taagtttagg gctcataaat aaatcttgta
     9601 tcatctgtgc tatttttaaa tctttagaag caactactac ggtagtagga atatcccttg
     9661 ctacttcttc agcatgagat ggcccagata gtattacata tggattttgt ggtaattctt
     9721 cttcacagac ttgagataat ctaagcccag tccctttttc aagaccttta gctacattaa
     9781 caataacttg tccttctttt ataaatggtt ttattttttg gcaagtactt cttacagcct
     9841 gtgatggaac tgctaaaact attaccttac aatcttttat agttttttct atatcagtac
     9901 ttatagttat attatttgga aataatactc ctggtaaata gtctgtattt tctttagttc
     9961 tgtttatttt tttagcttgt tcctcttttc gagtccacat gctcacatca ttaccttttt
    10021 ttgcaagccc taaagctaat gcacttcccc agcttccagt acctaataca catactttct
    10081 ccatgtttaa caccacctga gtataatata tttactattc tttcttttga ccaatttttc
    10141 tctctgtccc atttaataat cgctttatat ttgctctgtg attatatatt actgatgcag
    10201 ttaagaacag agctactatt aaaccagctt tattttttac cataatcata aatattggtg
    10261 aacatcctat tcctacaaca gaacccaatg aaacatactt tgttatagct accaccaata
    10321 taaatactgc tagacacatt agtgttatta ctggatttac agctagcatt gcccctagtg
    10381 aagttgcaac acctttgcct cctctaaatc ctaaaaccgc tggccaatta tgacctgcta
    10441 caacacatat aactgccaga tatccagcta aaagagtatc tattccaacc aatcttgctg
    10501 caaattctga tattaaaaca gccattacac ctttaagaac atcacctaaa aatgtcattg
    10561 ctcctgctct ttttccaagt gtcctaagta catttgtcga acctgcatta ccagaacctt
    10621 gagtccttat atcaacccca gcaattcgct ttgcaactat gtatgaggta gatatatttc
    10681 ctaaaagata tgcaactaca gcaataatta tataactaaa tatctccact gaatttcccc
    10741 cctttagtta tattttaaga tatttatttt ttattttttt gtctatactc aaactttatg
    10801 gaagttcctt caaatccaaa attttctctt atcttattct caaggtatct ttgatatgag
    10861 aagtgagtta aatccttatc atttataaac aatgtaattt taggaggtct aatgtcagtt
    10921 tgagtcccat agtaaatttt taatcttcta cctttgtcag atggtggttg atttaacatt
    10981 acagcttctc caattacttc attcagtgct gaagttgata ttctcttaga atgttcattt
    11041 gacacatatt caactgtatc tagtatcttg ttcattcttt gattagtttt tgcagaaaca
    11101 aatacgattg gagcatacat cataaatgga aatttttctt ttatatcttt tgtgtaattg
    11161 ctcatagtct tattatcttt ctctattaaa tcccatttat tgattacaaa tatgcatcct
    11221 tttccttcat catgtgctat acctgcaacc tttgtatctt gttctgttac accttctaag
    11281 gcatctatta ctatcaatac tacatctgct ctatctacag cactcatagc ccttattaca
    11341 ctgtattttt ctattgtctc atatatttta ctttttcttc taagccctgc tgtatctatt
    11401 agaagaaatt tctgtccatt tttttcaaaa taagtatcaa ttgcgtctct agtagttcct
    11461 gcaattggac ttacaattac tctttcttct ccaagtatct tattgagtat agaacttttt
    11521 cctgcatttg gtttacctgt tatagctact cttattatat cttcttcata ctctgtatct
    11581 aatcctgctg gaaaattttc aactatctca tctaataaat ctccaaatcc catactattg
    11641 gctcctgaaa ctgcaaaagg cgttccaaat cccaactcat aaaaatcgta tatattatca
    11701 aattgacttt gactatcaat cttattaacc actaagatta ctggtttttt tgtctttcta
    11761 agcaattgtg caatttcttt atcagcagct gttattccag ctttaccatc aactataaaa
    11821 agtattacat gtgacatgtc cattgcaagc atggcttgat ttctcatttg agataatatt
    11881 atatcttcgc tatcaggttc aattcctcct gtatccacta atgtaaaata tttatctaac
    11941 cattctactt cagcaaatat tctatctctt gtaactccag gtgtattttc aactattgat
    12001 atcctttttc ctgcaaattt attaaatatt gtagacttac caacgtttgg tctcccaact
    12061 acagctacaa ctggcctact tatactcatt ttatttctcc ttccatcttt tgttttctat
    12121 tttaaaattt tatccacaaa atcttttcct tcattcaaac aagggataat ttttattctc
    12181 ataagttctt ctaattgatt taattcaata ttatctaaga aaatttcttc atctgctttt
    12241 agcatacttc ttgttatata aagagcttct cctaattctt tatcttcaag ttgttctttt
    12301 aaatccttag ctgttaaaag acctgataca gttattgtct ctccaaaaaa cttgtttttg
    12361 atttcatata catttacttg aagattttta aacttgtcca tcatagcatc tgccatactc
    12421 tgtataaatt catatgcaga atgaccagta gctattgata ctttcttctc tctttttaga
    12481 atcttctcag gcaatatatt tagatactct tcaatctctg tttttaactt acgtatcata
    12541 cctacaccat cttcaaactg taaaaaacct tcatattctt catatccagg caattcacta
    12601 ttagatagta tataaaattc atctgataaa aatgcaaatc gagtacctaa tttttcaaga
    12661 tatttctgtt gtatttgatg tatttgctct atagttttac ttgcgctctt atcattaaat
    12721 atatttaatt ctactagatt ttctctatgc tttgtaatcc caactggaac tatcgcaaca
    12781 ctgtttacat acggataaag ttgagctagt tcttttattg ttctatccaa ttcctttcca
    12841 tcatttatac ctggacataa aacaatttga cagttcattt ctatatgagc atctgccaat
    12901 ctcttcatta taccatataa ttttcctgca aatttattgc ttatcatttt ttgtcttaac
    12961 tctggattag ttgtatgcac agatatattt ataggactta ttctatattt tataatatta
    13021 tttatatctt cttcactcat atttgtaagt gtgacaaaat ttccttgtaa aaaagataat
    13081 ctcgaatcat catctttaaa ataaagagtt tctctcatac cttctggcaa ttggtctata
    13141 aaacaaaaca tacacttatt tctacagctt ttagcttggt caattatcgg atttgtaaat
    13201 tcaacaccca aatcttcatc atagtctttt tctatctcgt atatgtagac ttcgccatct
    13261 tttttttgta tttctacatc taaatattca tcacttagta agaatctata ctctattata
    13321 tcatgtattg gctgttcatt cacagatata agtaaatctc ctacctctat acccatttct
    13381 tctgcaatac tatctttata aaccttactt ataatattat ttactttttt tacatttact
    13441 tccaatattt tcaccactct tttgtattca tcaatgctta ttataatgta ttttaatatg
    13501 caatttattt atttattata ttttatgata gcatttttaa gcatttcatg tcataataac
    13561 acattttaat tctaatagtc aatataagta aatagctata aaatagatta gaacaaataa
    13621 tattcaaatt taagtttctt aaaaatttaa atattatttg ttcttaaaaa aattttataa
    13681 atttaatgtt ttactatgat taacgtccca tttgtcatat catgaaccat tccacctaca
    13741 cactttgaca tcaacaatgc cttattttgt aagtctttct catcatttgc ataaaatatt
    13801 ggacaacctc caccattttt catattctta tcaagtgtaa ctatagctag ggtatattca
    13861 tttaaaccta tatccattcc catctcatgc acttcctttc tttcctattt tataattttt
    13921 tagtgataag ttttttcctt tagagcttga aagtattgga catgacttta cagcttccat
    13981 tactttttcc acattcttat ctactggtaa ataagcaatc attatacttt catcctcttt
    14041 atttcttcta ggtaatggta cgaatgaagg ttctccataa tctccatata aacctaacct
    14101 tgaatatata ttgtatacta ttgcttgtct ttgaccggag tcaaataata ttcctgcatt
    14161 agcataatcc ttatttttag gaattatttg tattcctaca cctctattaa tatatacttc
    14221 tctatcgctt tttagaccta tatttgttat accttttaaa tctcccacct ttagtataga
    14281 ttcgtccaca aaactaattt cagcaggcat tacatcagct atgtctccaa tactttttct
    14341 agtaagaagt ttttttaata taaaggctaa cactaatcca caaattgaac ttattaccaa
    14401 actcatccct tcacttattt tcgtttctgc aagtattaca taatacaatc ctattgttaa
    14461 aaaagatgta actatacaca tataatttct tacttcatag gttctagcta tctcttctat
    14521 gaatgcagct cctctcttta caagttgaac ttcctctaaa ttttgcaatg tatttcttct
    14581 atttgctctc acttgtctaa attgttctgc tgctagagat aaaaaagtta tagatgtata
    14641 agaacgattt ataagagctg gaaccaataa tgctcctaat gaagaagcga cgaatgctaa
    14701 tataagttga gatagtaaaa catttggttg cgttggatac tgtttttggt ctaaattcaa
    14761 cattataatt ctagatgtta taccaataat aacagcaaca aaaaatactc tatccattaa
    14821 atcactccta aaatatttaa tttgaaattt tacatgtaaa tctacaaagt ctttctagaa
    14881 aaatcactct gctttccttt tgatgtttct atgataggtg tacttttcac tacatctatc
    14941 atgacatcta tatctttgag tattggcata tatggtataa ccacagtact tttttctaaa
    15001 ttggtcttag atatagcaac aatatcaacc tcatctacat ctctatctat ccccatatga
    15061 ataaatagat tatgtattat agcttgtctt tgccctatat catttactat tccaaatgca
    15121 cctaaatctc ttggtataac ttctatggcc agacattcat ttctatactt ttctcttgta
    15181 ctttctagac ctatgttagt tattattaca ccatttacca ttaaaatagg tccttcaaaa
    15241 cttatttttg caggaactat atctgctata tctgctatac tgttccttct taaaaatctt
    15301 ctaaaaatca aaccaacaat agctcctgac acaatagcta ggattgtgca ataaaaaaag
    15361 ctaaggccat actttcttgc aaaaacgata taaactatcg aagataccag tgcagaaaaa
    15421 agacttatat aacttctaga ttcatacgta gatgctattt cttcaacgta agcgtctcct
    15481 tttggtacta attcttcatt atcgatattc tttagtgtta ttctttcttg ttctgcaaga
    15541 ccttgaaact gttgtattgc tactgcaaaa aatgtcaagg ctgcaaactc cttatctata
    15601 agtgcaggta atgctatagc acctaacgaa gctgaaagcc cagatataat tatctgttct
    15661 aaataatctt gtggtcttga tgggtattgt ttgtctgtaa ctcttaaaac taatcctcta
    15721 cataatattc ctacaactaa tgcaactata aaagagtgtc taaataaatt tgcattcaat
    15781 aaactttctt ccattttctc agctccttct cttatatagt ttgtgcaaaa aacaaaaaaa
    15841 attcccttct atttaaggaa atttttttgt aatattgtaa ctataaattc gctaccaatg
    15901 ccaagttcac tttttacttt tatacatcca tttagtgatt tgactatatg ctttgttata
    15961 gccaatccta aacccgttcc tcctacatcc ctacttcttg ctttatctac cctataaaat
    16021 cgttcaaata tgcgttctac atcttctttt ggtatcccta caccattgtc tataacttta
    16081 attacgattt tttcttcatc ttttaacact acaacttgta caatcccatt atcttgggta
    16141 tactttatag cattatcaac taggtttaag aacacttgct ttatatagtc tttattggat
    16201 agtatactta ttgatttatc atcacattta tataaaactt ctatattttt ttcactagct
    16261 gaatgttgag ttatttcata tacctcttta aatacatcat atactacaac attttccata
    16321 agaagagtat ctttattttc tacaaatgat aacagcaata tatcatcaat caaccttttt
    16381 aatctatctg attcgctttc tattatttct aagaatctat ttctagtatt cacatctata
    16441 ttttcatttg actttagtgt ttcaacaaac ccacttatag atgtaagtgg tgttttaagt
    16501 tcatgactta catttgcaac aaaatcactt ctcatatttt caagtttaac ttttttagta
    16561 atatcttcta tatttactat cgaacctatt attacgttgt tagtattctg aagataaact
    16621 gggtctactt ttatcttata tacgatatca tctttagtag taatattagc agtttcatta
    16681 tctttagaac caataaattg aagtatttgt tttaatattt cttcttcttt tattacaaca
    16741 ttaatattat gaccttcaac taattgattt ggttcactct ttatcatttc ttttgcttca
    16801 tcatttatca gaagtatatt tccatttaca tctatagcca aaattccatg agatatactc
    16861 tttaatatag accttaactg taaatgttta tactgtactt cttctaaggt tttatccata
    16921 ttctttatca tatgattaaa atttctagct aattcgccaa gttctccttt ggctgatatg
    16981 ttaagcctag catgatattc attattactt actttttttg agacgtatat aaactctttt
    17041 aaatatttcc ttaatgttat tgtgtatctt acagctaata taacaatggc tatggacaac
    17101 ataacaataa aaaaatatat actatttaaa tcttccacaa gataacactc ctaaaatata
    17161 acctttttaa tctattttgt atcctacacc tcttatagtc tctatatatt tttccgattt
    17221 atcttcatct tctatttttt ttcttaaata tcttatatga acatctactg ttctagtttc
    17281 tccatagtat tcataccccc atattttatc caaaagataa tttcttgaaa gtacttttcc
    17341 tttattttga attaataatt ttaacaattc aaattcctta agagttaact ctatcttttc
    17401 agagcctttg gtaacttcat gttttgataa atctagtttt aaattacctg tagtaagtat
    17461 attgttttct tcacctaagc tagtcaaatt atatctcctt aaaacagaac taatcctagc
    17521 taacaactct tttatactaa atggcttagt tatatagtca tcagcaccta tttcaagccc
    17581 ttcaaccttg tcattttcca tatttttagc agtaagcatt ataacaggaa tattttttaa
    17641 gtccttatca cttcttattt ttctcaaaac ttctattcca cttatatttg gtaacatcca
    17701 atctaaaagt attaaatctg gtttaatttc cttagccttt ataaatccgt caaatccatc
    17761 atatgaataa ctaacttcgt aatttgacac ctccaaattg aattttaata gctcaactat
    17821 atgcatttca tcgtctataa caagcacttt agtattcatt aaaaagctcc tctctatttt
    17881 atctgtagta ccgttcctat tcgttctgat gccttattat gttttctata agagtggaat
    17941 ttatctgctc tacaactagt gcataaattt aaatttatta tattttcttt tttaactcca
    18001 ctacatctaa gcatatactc attaattttc cataaatcta aatagtaact tccctctttt
    18061 attatataga acttttcatc attatttgta agaattgtgt taaatttttc tactaaatct
    18121 tcagacactt cataacaaca tggtccaatt gatggcccaa ttatacatac taaatcttca
    18181 ggaactgttt tatagcattc agacattaat tttattgttt tatgacctat attactaaaa
    18241 gtccctctcc aacctgcatg agccagtcct atggctttat ttttagcatc tataattgat
    18301 ataggtacac agtctgctgt aaacagtaaa agtggtactt tttccaagtt tgttataagt
    18361 gcatctccat ctcttctttt ccctatagta tctttattga ctatattaac tatatctgaa
    18421 tgtatttgag aattactagt tagattttca aatgtcaaat cacattcttt acatactttt
    18481 aacatatctt ctctattttt tgcatctata ttcttacatg ttataactag atttacagaa
    18541 tttaattttt catcaagttc ttcacaatta ccacttaaat taacatactc tttaatatct
    18601 tgtacatttt ttatatttat ataatctttc atcatttgtc tcctttttct atcagtatac
    18661 ttttaagttc atttacaaaa gtatttatat ctttaaattg tctatacaca gaagcaaatc
    18721 ttacataggc aacctcatct aactctttca atttatccat taccatctca ccgatttttt
    18781 tggtttcaat ttcttctata aaacatttat ttatttcatt ttcaatatgt acaacaatac
    18841 tttcaatctc gtcaatagaa acaggtcttt tttcacatgc ttttagcaat ccatatttta
    18901 tcttttctct atcaaaatat tctctactat tatccttttt aatgaccatt aaaggagttg
    18961 tttctatttt ctcatacgta gtaaacctct ttttacatga ttcacactca cgtcttcttc
    19021 tgatagattt taaatctgta tgtctagaat ctattacctt tgattcttta taattacaat
    19081 aaggacattg catctatatt cctcctttgt ttaactatat ctcaaactca tctaagctta
    19141 attctgaacc tatatttact attattgtat cacaacctat tttaagtata tcattccaaa
    19201 atataacttc ttcatcccct cctcttgaaa aaaaacttct acttccatct ccaaaaatgg
    19261 taaatgagac taccttacct tcgtgaatat ccatgtctat gtctattata aaccccatcc
    19321 tttttccatt ttttacattt ataatttctt tttccattat atcagaaact ctaatcatat
    19381 tgtactcacc ctttctttaa aaataaatat gtatcgaagt aaaaaaaaat gacctatata
    19441 ggtcatttaa attaaaataa tgtttacagt ccatggatta cttatacata ttttctcata
    19501 ttttttaaag catttttttc aattcttgat acttgtgctt gagatatacc gatttcatca
    19561 gcaacttcta tctgagttct tcctttgtaa aaccttaaat ctaataccaa cttttctcta
    19621 ctatttaatt tcttaatagc ttctttaagt gatatttctt gcaaccaatt ttcatcagta
    19681 tcttttttat cttgaacctg atccataacg aatattgcat ctccattgtc ttgatatact
    19741 gggtcaaaca gagatattgg gtcttgtatg gcatcaagtg ccattacaac tgattctact
    19801 tctaattcta attcctttgc tatttctgat actgtaggtt ctttagaatt tgttctaatt
    19861 aatctttctc taacttgtag tgccttatat gctatatctt ttaatgacct acttactcta
    19921 attgggttat tgtctcttag ataccttcta atttctccta taatcatagg aacagcataa
    19981 gttgaaaacc ttacattttg gcttaaatca aagttatcta tagcttttat aagaccaata
    20041 caacctattt gaaataaatc gtctatattt tctcctctgt tgttaaactt ttttattaca
    20101 ctcaagacta atcttaaatt ccctcttaca aactgttgtc tggcttcttc atcaccattt
    20161 tttatttgta ataaaagttc cttcatttgc ttatttttaa gaactggaag ttctgatgta
    20221 ttaacaccac atatttcaac cttattaact tgcatatatt ttcagtcctc tctcaaaagt
    20281 attcatctat tgaaattatt tacatttcca atttttttat actttttaag aagaattaat
    20341 ttagcattat acaaattttt tcatttcttt ttgcaacctt gagattattt tcttttcaag
    20401 tctagatatg taggactgtg atattccaag cattccagca acttcttttt gtgttttttc
    20461 aataccttta tcaataagac caaatcttaa ttccataatt tgcttttctc tgtcacttaa
    20521 tctgtcaagt gccataacca acaaatctct atctatttct tcttcaatta ttttgtatat
    20581 ttcatcattt tctgtaccca atacatcaga taataaaagt tcgtttccat ctaaatctat
    20641 atttaaaggt tcatcaaatg atacttctgt tttcttttta ttattttttc ttaagtacat
    20701 taatatttca ttttctatac atctcgaagc ataagttgcc aattttatat ttttattcag
    20761 tttaaaagta ttaactgctt ttatcaaacc tatagttcct attgaaatca aatcttctac
    20821 atctatacct gtattttcaa attttctcga tatgtagact actaatctaa gatttctttc
    20881 tattaaaatt gactttacac tttcatctgt ttctagcttt tgtaaaagct ccatttcttc
    20941 ttctggtttc aatggaggag gaagtatatt tgcccctccc atatagtata ttccctttgg
    21001 ctttattatt tttaatttaa tacctaacat agtaataaag cttattattc tttctttcaa
    21061 tcgtaacatt ccttgtcccc cttaaatttt cacctaaaat ccaggcaata tatttggatt
    21121 cattattgct ccatattctc tatcattaaa atttgaaata ccaagtatta tatttcctat
    21181 cttttgctca tctacttcta tatagtcagc ttttaagcct attattatgc ttgtcttatt
    21241 acttccagca tgtttataag gtattattct aactctacct gaagttttat catctaaact
    21301 tcctataatt tcttgtgctt tcttcatatc tatatcttca taattaatat ttaatagttt
    21361 ttctggaata aatccttcta acacactaga cttaactatt attacatcac tttgacttat
    21421 tgggtctttt aacaaattac cactatctac taaagcctta cagcaaaaac ttttttctag
    21481 taagtttata tttatagtct ttgtaagctc cttaatgtac ttaagcattt tcaaatcttt
    21541 ataaatgtat tttaataatt cacaactcac ataagtacat gcaattaaga aagatatttt
    21601 taagtgactt attcctgtaa aataaattat aaaataagtg cttccagaaa taaaaatgtt
    21661 tactagataa aaaactaacg ctacatgaac ataatctttt ttgctcttac ttctaaatga
    21721 aattaaagtt attatactca tgattattat tttaaatggt acagtaaata gtatattcat
    21781 tgatggatac aaataagcaa cagaataggc acctcctaaa ctagccccca ataacttatt
    21841 ttttttagga ttatatcttt tagtaagaat agaagtacaa cttataataa tataatttat
    21901 tactaggttt tctataacat agtactcaat atacaccatg tatcccccct taaaaccttt
    21961 aaacatatta taagatagta tgtacaaaat tttatgtcac ttttgatggt cgaatccaaa
    22021 acaaattcta tttaaaaaga acaatgatat atgtcttata ctttatgtcg tcaaaagata
    22081 tttttttaat accaaacaaa tagacttttt tgtagaatcc aatatatcta ggtagaagtt
    22141 attcactcta cttttggtat atttacaaaa aaagactaca taatttaata atatatcaag
    22201 taaatctttt acttatataa aatcaaatta tatagtctgt aaattcaaat ataaattatt
    22261 gtgctagtaa tccatttgca tattttgcag attcatttag tgcttgttct ggagttatgt
    22321 ctccagctat catcatttga agttgtttcc ataaatattc atatatctct actccatgag
    22381 gtccaactgt caatggtcta tacatatgtt tatctcttgt caccatttct tcaaatcttg
    22441 ggtctccttg gtatttttca cctttagcaa ctggagctct aggtatttct ttgtggaaag
    22501 cttccattga ttctttgctt agcatatact caagaagttt aaatccaagt tctttatttt
    22561 tacatgctga cattaatgtt aaatcatcta ctgaagcaaa acttccaact gtcttatctt
    22621 ctaatgatgt tacatatccc cagtttaaat ccgggaatga tttatcaaaa gtacttgtag
    22681 cagcagagga tagcattata gtaaatgcag cttttcctgg tccaaataca ctttcaatca
    22741 tttcattact atcttttgat aaagcatctt ctggaatata ctttttcata tcatttataa
    22801 agtttgcagc tttcaagcct tctgcattat taaatttaac tgattttaaa tcatcattat
    22861 atatatctcc accagcttgc cataaatacg gataccagtt ccagtttaag cttccaaata
    22921 ctttagaacc ccaaccttgt gcaagacccc actggtcgat ttttccatct ccatcagtat
    22981 cttttgttgc tttttcacag attcttttaa aatcatccca tgtttttgga ggtttttctc
    23041 ctaatttttc tagtatatct ttattgtagt acaatacgcc tggattagca gcttctatag
    23101 gtaaaccata aataccaccc atcatttttg catcatctaa gtatagataa ttatctgaag
    23161 ttcctgattt ttctaaataa ggtgttaaat cttctaccgc acccatttct ataaattgag
    23221 gaaacatctc tgcatacatg tatcctatat ctggaccttc acctgcactt attgctgttg
    23281 catatttttc tgcgtagtta tcccatggta ctatctgaaa ttcaatttca cagttatttt
    23341 ctttttcaaa tttttcaaat atcggcttcc atgcatctgc atcatggtct gttaatggtg
    23401 cagaccatac aactaatttt tctttaccct ctttgccttt agaatcttct ttttttgaac
    23461 atcctgcaag aattgatact gatagaatcc ctaccataac taaagaaaga acctttttta
    23521 gtttaatcat gcttaatccc ctttcagact ttaatttgtc tttttcctca aatgtttaca
    23581 tttaattcag atttaatatt aacaaataag attactcttg tctaatcata aaaaactata
    23641 ctaactccta taactatatt ttttttctat acctattaga tttaattcat actaaaaact
    23701 tactttttaa attttatcaa tatttaaaaa tttttttaag tctagccaca aactaactat
    23761 ctaaattgag caaaataaaa agaagtctat tgacttcttt ataagcttac atattatctt
    23821 cttcttctta ggaatgtagg tatctccatg ctctcatcat catcttcttc aattatactt
    23881 gcctttttag gaggctctat tttttcctca gcaattactt cttcttcaat ttcttttaca
    23941 gcttgtttta ctgttgtatt taatgaagaa cgtatgcttg atttaggttt agtatctaaa
    24001 tctaaggcac cattttgtaa tccttcaaat cctgtcgcga ttactgtaat cattatttca
    24061 tcacctaaat cttcttttat agaagctcca aatataacat ttgcttctgg gtcacaggat
    24121 tccataacta aagttgatgc ctcattaact tcaaataatc ctaagttagg accacctgta
    24181 acattaagaa gtactccttt agctccttgt attgatgttt ctagaagtgg agattgaata
    24241 gcttgtcttg cagcatcaat tgctctagtt tcaccactag cactacctat acccatatga
    24301 gcaagtccct tgtctttcat tatagtagta acatctgcaa agtccaagtt tattaaccct
    24361 tcaactgcta taaggtctga aattgattgt ataccttgtt ttaatacatc atctgcaact
    24421 gcaaaagcat ctaacattga tgtatttttt tgtactatct gtaaaagtct atcatttggt
    24481 atcgttataa gagtatctac ttttgattta agttctgcta ttccaccttc agcgttcttc
    24541 attcttatct taccttcaaa tgcaaaaggt ttggtaacta cacctactgt aagtatcccc
    24601 atttcttttg ccaagcctgc aaccactgga gcagctcctg taccagtacc accgcccatt
    24661 ccggctgtta caaagaccat atcagctcct tgaagtaact ttactatttc atctttactc
    24721 tcctcagcag ctcttttccc tacttctgga tttgctcctg ctccaagtcc tctagttaat
    24781 ttttctccta tttgaacttt atattcagct tttgatgtat ataaagcttg tttatctgta
    24841 tttactgata taaattcaac gccttttagt tgagcttcta ccattctatt gacagcattg
    24901 tttccaccgc cacctacacc tattacttta atttgtgcac attcttctaa ttctacgtca
    24961 aagtttagca tcataaaccc ccttctctca tgattcaaag ttatcgtatg tattaattat
    25021 atcattttct tcttttattt tgttaacctt taagtaacat attccacaaa aacgtaaatt
    25081 ttccttttta aatttcttac ttatattgtc aaatattact ctttaagtat aaaataattt
    25141 acgtatatat taaatgctct ttaattttta cgttttttgt tcataatatt actaataata
    25201 tatcgtctaa ttatagataa gttattaaac aatctcatac caaaaactaa tacagcaaca
    25261 tagtaaagag aaactcctaa cttttctcct atataagtca ataatatagc caatatagca
    25321 ttacctatga aaccagtaat aaaaattaca ttatcatagt tatcttcaaa attggatttt
    25381 atcgctccaa aaatagaatc cattattgca agaatagcta ctgacatata tatggaataa
    25441 tccatagaat atgtaaatgg aatatagtaa cctactacta caccaaatgc taatcctcct
    25501 aaaacccata acataaatct tcacctcttt attttacttt gaaacacaac tacattacaa
    25561 tcctttttat taattttttt tactttttct ttattttttt gtatttttcg ttttattttg
    25621 caaataaatc cactttattt ccattatttt cctgaacttt tatattaatt ccatatatat
    25681 ccttaagaga tttagcatat gattctggag agtttatagc tgcactcaat aaatctatat
    25741 ctcctatagc ttttattata aatggttgtc catatgtagt atcatttact gtaatagtag
    25801 ctcctgaaca ttttatctta cttgttgcta gtactctttc tccatttata gaaattgctc
    25861 ttgctccagc cttttttaaa tcatttaaaa ccctcaaaat atctatatca tgtataatca
    25921 aatcgtttgg attctgtccg tcttttaact ctctctcact atccctcata gtaatagtta
    25981 tgcctggccc tgtaacagtt gaataaccac ttaattcttt aacttcttca agctcttttt
    26041 gcataagtgt ttggatagat tcatttccat tcttttttgc ttctttatat tcattcaaag
    26101 tttttttgtt gtttttaaga gtttctttta aattttttat ttcttttttt gtattttcta
    26161 cctcactttc aatactttca gtttgaccta atgttatgta tactctattt gggtctagtg
    26221 attttatata agttgatatg aaaacgccta atatgaatgc tcctaatata actattttct
    26281 ttttcatact attatttccc ctctgctttg tttatatcac tatgaccatt tatttctata
    26341 ttgtcttctt tggtcacttt tactttggca ttataatatg tttctatttc ccagacaata
    26401 ccatacttta tattaagtgc cgaagccaat gtatcagaat caccaattgc ttttattata
    26461 attggttctt ttatgacagt gtcattcata taaagcttgt cttcttttaa atacatatat
    26521 gtattagaaa ctattctctg gtcgtttata gatatggcac ttgcttgtgc tgaatttaat
    26581 ttatttatca ttgacaataa taaatcataa ttatatagta tactatcatt tttatcagag
    26641 tttcctaagt cttttgtaga atttatctca atttttatgc ctgttccttc aacatctgta
    26701 tatccagcta atgtttcata tttttctatt tctgacttaa gtatgtcttg ttctccttca
    26761 ttgtttttgc ctctataagt ctctatatct ttcttcacac tattaacttc tgattgtaga
    26821 ttctcttttt ctttttttaa agatttaagt tcttctgtaa gttgctctcc tcttttaaaa
    26881 gttgtcatcc ctctattttc tatattaata attttaagtt gtaaactaac aagaacaccc
    26941 aatacaatac ttcctatgat tatactttta tttataaatc tttttttcaa tatatcactt
    27001 ccttacaatt gtctatcctt ctggactata cactgccaac tttccatagt taagatttat
    27061 agtccctcca ttagtatttt tattttgcag gtcaatcaga attttactta ccctagatat
    27121 attataatct aagttatcat cattagaaag taagattttt atattacttc ttgtaagcat
    27181 ttctatattg ctttcttttt ttaagtttac ataatttatt tttctataaa ttccttcttc
    27241 ttttaaataa tttaaagttt taaatacatt ctccttagtt ttataatttt taaattttat
    27301 agatttatta tcatcaatgc tataatctac atctactatc aagtcttttt tactttcatt
    27361 agaacctctt agttcttcca ataaatttcc ttttttatct atataacaat aattatcttc
    27421 atcttttaat actgcaaata tttctttttc ttttaaactt ataattatct tattgggaag
    27481 ttttcttttt atttccacat tttctatata tggatttttt ataagtttat ttttcatatc
    27541 tttaaaattg tatgcaaaaa tattttcatt tagatttaca tttagttctt tcattatatt
    27601 tgatttggtc accctcttat ttccaattac atctattttc tttacatcaa ataaatcact
    27661 ttttacaatt atacatatac aaaaagataa cataagtata aaaactaaaa ctatcatgac
    27721 attattcgta tttaactttc ttcttttttt caaatataat caaccccttt ggttaataaa
    27781 tacaccttta gagcttgttt ttcctacaaa aaaattataa tatttaaagt caaatttttg
    27841 cattttatta cataacatcc aaataaggta ttaaatttta taaataaatc aatatttcat
    27901 caaaataaac atagcatact acatactatt tatatattta aatctgtata catacaaata
    27961 aacccctaat ttataataat ttagaaaatc taatatatta taaattaggg gtaaatattt
    28021 acttatcttt tctttattcc tattacctta gcttgacctt ctatacttgg agtcgtttct
    28081 ttttttattt cttttacttc cttaataact ttttcttttt ttgttttttt agaagttgac
    28141 ttttgtgttg aattgtatac tttcattatc tcatcatata taaggtctat ggcttcaggc
    28201 ttacctatag ttttacttgc atttgccata tcaaccaaaa gttctctatc tcctaaaagt
    28261 ttaaatactg ctgtatttag gctctctgga gttagatttt tttctaaaat agcaattcct
    28321 gccccttgtt tttctatact cttggcatta tactcttgat gattttcagc agtataagct
    28381 ttaggtatta ttatagatgg ctttccaaga gccgttatct cagctaatga aattgctcct
    28441 gcgctaccta taaccaaatc acttgctgca agtgcatttg ccatatcttc taagtaaggc
    28501 acaacttttt ggtatggttt aagatttata tcgctaatac ttcccataaa ttcatcataa
    28561 tagcttttgc ctgtagcaaa tataaaagct atatcttcat ttaccatatt ctttattaca
    28621 agtctcattg catcatttat ttttcttgaa cctccactac caccatagca aagtaccatt
    28681 cttttttcgt cacttattga tagattcttt cttgcaatat tttttcttga taataaaatt
    28741 tcttttctaa ctggatttcc tgtaaaaact aatttatctt ctgctgcctc tggaaatctt
    28801 ttatgcgaat cttcaaaact tgtaagtacc tttgtaactg ttttagataa aatcttatta
    28861 gttacaccag gaaaagaatt ttgctcatgt attatggctg gtattttccc catagatgca
    28921 ttaaataaaa caggtccact aacatatcca ccagttccta taactatatc tggtttaaat
    28981 ttcttaacaa tctttcttga ttgctccaaa cctttaaaaa gcttaaaaac tctttttaca
    29041 ttatcgaaat ctatttttcg cttaaaacct tgaactgtta cagtctttaa ttcaaatcca
    29101 tacttaggaa ctatttctga ctctattccc ttttccgtcc caacaaaaat tatctcagcg
    29161 tctggatgct catctcttat tttgtttgca atagctatag ctggatacac gtgccctcca
    29221 gttccaccgc ctgataataa aactttcatc aaatcatcat ctcctgttaa ttaattttga
    29281 catgctttga gatgtttaac actattccta cagctcccat aaagatggtg agtgaagttc
    29341 ctccataact tataaatggt agtgctacac ctgtagctgg cattgatgat gtcgcaactg
    29401 ctatatttaa agctgcttgt attccaattt gtgcacctat acctataaca accatacaag
    29461 caaatacatt agaagtcttc aaagctattc ttacacatct gtagactaaa actacaaata
    29521 gcataataac tattatacaa ccaatcaatc ctaactcttc tcctattata gcaaatataa
    29581 agtcattttg tggctctggt atataaaaat acttttgttt actctttcct aagcctagac
    29641 caaacaatcc acctgaacct agtgcatata gaccttgtat tacctgatac cccttaccaa
    29701 gagggtcttg aaaagggtct aaaaatgatg taacacgact aagtctatag ggttcagcaa
    29761 ttactaatgc agcaaataaa gcagccccac tccctatcat agcaaataca attttcatat
    29821 ccattccagc aacaaatatc attacaaaag taactagaat tacagtacct gctgttgaca
    29881 aatttggctg aagtattatc aacgcaaaaa atatccctgg aactacgaga agtggtacaa
    29941 caccttttgt caaagatttt attttatcat aatttttttc aattaatttt gcagttaata
    30001 ttattgtagc aaactttgcg atttctgcag gttgaaatgt taaagctcct attcccaacc
    30061 atctttttgc tccgtttgct tctatcccta aaggggtcaa aactaacaat aataatacta
    30121 ctgctatagc tccaattgct gtagcatttt tcttccaaaa actataatct attcttgatg
    30181 taataatcat aactataaat ccaagtacag catatattac attctttttt agaaaatagt
    30241 acgcatcatt gtgtttaaat gaagattgta taaaacttgc actaaacacc ataactattc
    30301 ctacaaatac taaaagcata gtagtgtaaa acactactcc atcaaattca gtcttcaccc
    30361 ttatgtcaat ttgttttttc aaattttctt taggcatcca actaatctac ctccattctt
    30421 ccatttaagt aagtttagag gtaagagatt attttaaatt gtttacatta tctttgaagt
    30481 cttttcctct tacttcaaaa ctttcataca tatcccaact tgcacaagct ggtgaaagaa
    30541 gcactatatc tccacttttt gaaatttcat aagaagtctt aactgcttct tccatatctt
    30601 ttactatatg tatgtcatta aatcctttat ttttagcaca attttctata tttgatgcag
    30661 tttctcctaa taagaccaat gatttaacat atgactttgc agtttcaaga agttcatcaa
    30721 aattacttcc cttatccatc ccccctgcta taagtattat aggtctatca taagactgta
    30781 tagcttttat agtggaatct ggattagttc cctttgaatc atttacatat attacattgt
    30841 ctaaatttct tacaaactct tgtctgtgtt ccactccttt aaatgtcata agtacatcct
    30901 ttataacttc taaatcaatt ttgcagacat atgctattgc tatagctgcc atacaatttt
    30961 ctaagttatg acctccaggt aaacttacat catctttatt tagaaatttg attttatcat
    31021 ctatatctat tattatgtca ttattttcta aatatactcc tccatttact tttttagttc
    31081 ttgaaaagta aattactttg gcattgcatt tatcagctaa agattttact atctcatcat
    31141 catagtttaa tacacagaaa tcctcaacag tttggttcat aaatatattt gcctttgctt
    31201 ctatgtattt ttccatagta tggtgtctat ttaaatggtc ctctgtaata tttaatattg
    31261 cgcttacttt aggtctaaat tcatctatac tctcaagctg aaaactgctt agttcagtta
    31321 ctaacacaga ctcttcactt gaagtttcta tagtatctat tacaggattt cctatattac
    31381 caactacata tgtatctcgt tttgctcttg aaaaaatttc tcctaccaaa ctagttgtag
    31441 ttgtctttcc atttgtacct gtaatcccta taaaaattgg attatccgat agtttaaaag
    31501 ctaattctac ctcaccaatt atatacttgc ctgaattttt taatttcaat atgaatggta
    31561 ggtctagtgg tacacctgga gacactacaa ccatatctat atcatctaca tcttctgggt
    31621 gatgtcctaa tatgtatttt atgtcattta tagatttcaa ttcatcaagt atatttctca
    31681 atttattttc atcttttata tcatttacga ttatacttgc acccaattta tctaaatgtt
    31741 taattgtaga tataccagtt tttgcaagtc ctactaataa aacttttttt ccttttaagt
    31801 ccatttttca ttccccctag ccctaaaata ctgctattat acttatccaa gctaaaacaa
    31861 ctgttataat ccaaaaaata aatacaactc ttgtttctgg ccaaccacac tgttcaaagt
    31921 ggtggtgtat tggagccatc ttaaagaatc tctttcttgt tgccttaaaa tatccaactt
    31981 gaatcaaaac tgatagagcc tccgcaaaat aaattcctcc aattataggt attataagca
    32041 ctgagttagt tagaactgaa aatgctacaa ctgcacctcc taatgccatt gaacctgtgt
    32101 cgcccataaa cactctagcc ggatatgaat taaatcctaa gaaacctaaa catgctccaa
    32161 cagttgctgc tgctaaaact gcgacttctg tatttccagc aatagaactt gcaaatagca
    32221 taaagaatac agaaactatt aatgttactc ctgatgctaa tccatctaat ccatcagtca
    32281 agtttacagc atttactatt gctacgatta taaacatcat tattggtata tataatattc
    32341 caacatttat cacaaaatct gtaaatggta tcataagttg tgaagcactt gatgatgatg
    32401 tatactgata aaaagcaaca taaaaagata atgcaacttg aaggattatt ttttgatatg
    32461 cttttagtcc aagtgatctt tttagcttta tttttataaa atcatctaaa aagccaataa
    32521 aaccaaaacc tgcaatacat ataagaccta ctgccatatc atgactaact tttactcttg
    32581 taagtccagt tattagaatt gcaacaatca ttataattcc acccatagta ggtgttccgt
    32641 ttttagctag atgtgtttgg ggtccatcat ctctaactgt ctgaccaaat ttaaattttc
    32701 taagcattgg tataaatatt ggtcctatta taattactat taaaaacgca atcaatgcgg
    32761 tgtacgtaag ctctgttatt cctaacatca taaaactcct ctcccttgtc aattaactca
    32821 ttagtttaag cttatttatt tagatattcg actatttttt ctaatctcat gcctcttgag
    32881 ccttttacta aaatagtgtc tcctgttttt actaattcat ttaacttgtt aaacacatct
    32941 tctctattct caaagtgata tatattggct gaattgaatc ctaattgttt tgcttcttct
    33001 ccaataaaca ctgaattttc acctatagtt attataatat cagtattatt catagatgat
    33061 tttccaacta atctatgacc atactctgaa atttcaccca tttctagtat atctccaagt
    33121 atcgctaccc ttctaccttt atatcttcca agtatcttta aagcagcatc catagaatct
    33181 ggactagcat tatatgaatc attaattatt gttaattttt cattttttat tatgtctagt
    33241 ctcatttttg tccctttaaa attttttaaa ccttttttta tataatctaa agaaatattt
    33301 agacacaaac caactaatat agcagacatt gcattatata tattatgttc tcctattgtt
    33361 ggtataaata tttcctcttt tttcccatct attacacata taaatgttaa actatcctct
    33421 tccatagtat aactttcaca ataaacatca ttgtttttat taaagccaaa tgtttttaac
    33481 ttatatacat gttctttttc ttttaatgta gataaaaact tatcatctcc atttacaatt
    33541 aaagtatttg tttcatcaaa gtttgaagta atctccatct ttgctttcaa aataccctct
    33601 tgtgaaccta gtttttctac atgtgataat cctatattcg atatgactgc aattttagga
    33661 tttactatat ccactaaata ttttatttca ttgaatccag acatacccat ctctattaca
    33721 gcacactcat gttctttatt tagattaaat aaagtcaatg gaactccaaa atgattattt
    33781 aagttttttt cattttttag tatatttaac tttgcagata tggcagagta aatcatatct
    33841 ctagtagtag tttttcccac actacctgta acacctataa atggaatatc aaatttttct
    33901 ttatagtatt ttgaaatatc tcctagagcc aaagatgtat cttttacctc tattaaattt
    33961 atatccgaac tttctaattt tattccatta ctttcatttt ttataaaagt tttacatcca
    34021 ctatcatatg cagacttcat aaatgtatgc ccatctaaat tttctccaac tatggctaca
    34081 aatgcatttt gggcatttgc ctccctgcta tctataacta tatcacttac acagtcatta
    34141 taatctccat atattaactt cccattagtt gcaagtacta actcttttat tgttaagcac
    34201 tccattatta aactcctcct ttaaaacctt agctatattc tcttagcaac aaaaggcaat
    34261 gtataccgtt atatatacat tgcctacgat aactcttaac tttataatta tatactatat
    34321 ttctgttttt ttgaagatgt ttatgggtat atgttaatta tctgttactt tttagttttc
    34381 aaaatatagg gttatacttg agtcttcatt aacttttact ccaggttttg ggaatatatc
    34441 ctttaccttt gtatcttctg gaagttctat atctgcatct aagtctggtt ttaacttcgc
    34501 atcctccaat gcctttacag catctcctat ttttagattt cttacatctg gcactttaac
    34561 ttgcttcttt tcatactcag ctttttcttc ttctttatat actggtttaa ctcccaaata
    34621 ttttaaagag tcattcatta tttctttcac tattggtcct gctgtagtac taccaaatgc
    34681 acttactcct gttggttcgt caacaatagc aagaactact atttgcgggt catcacaagg
    34741 tgctattcca acaaatgaac atatatactt tcctggagca tacttaccat ctataacttt
    34801 ttgagctgta cctgttttac caccaagtct ataacctggt atatacgcta tctttcctcc
    34861 accttcagtt acaactgatt ctgcaatttc taacattttt ttagatgttt ctttagatat
    34921 aacacttctt actttttttg gttttactgt ttcagttata tttcctttat tgtcagtata
    34981 agattttacc actcttggtt gcattaaatc tccaccatta gctattgatg atatagctgt
    35041 tataagttgt attggagtta ctgatataga ttgaccaaat gatattgtag ccaattcaac
    35101 tggtcctaca tttttttcgt tatacagtat tcccttagct tctcctggta aatctatacc
    35161 tgtcttatcc ataagaccaa atgattctat ataatcatac atttttccaa cacctaatct
    35221 gcttcctaac tcaacaaata ctgggttgca agagttttgt actgcttgtt taaattcttg
    35281 tgttccatga ggtctgtaat gtctccaaca tttaatcttt cttcctccaa cagttacact
    35341 acctgtacat gtgaattttt ctccatcttt aattactcct tcttcaagag cactggaaga
    35401 tgtaataagc ttaaatgtag aacctggttc atatgtatca cttactgctg gatttctcca
    35461 catttgataa tagcctttta ttttatcttt atcattatat ttttcaagtt cttcttgata
    35521 atatggatat attggcgtcc ttgagtcatt agggtcataa tctggttttg atgctaaagc
    35581 aagtatatca cctgttttag ggttcatagc tattgcagta accttttttg cattatttaa
    35641 ctcataagct ttctgtactg ctttttcagt atagtgttga attacttcat ctatagacaa
    35701 cacaagacca ttaccttgta caggctcata atatttttcc attccttgtg gtatttctct
    35761 tcctgaagca tcagtactta ctataagttt tccaggcttt ccttttaact ttttatcata
    35821 ttgcatttct acaccagata tacctgttgc atctgaagaa gtatgtccta acacatatgg
    35881 tgcaaaattc ccatatgggt aatatctttg attatcttct gccacccata ttccacttaa
    35941 tttagcatct cttatttgac ttgccttatc atcatctatc catcttttta cttttacaag
    36001 agccatattt tttttgctta ttaaagcgta aatatcttta taatcttcat ccaaaatctc
    36061 agctaccttt tcggctgctt cttttttatc ttctacttcc acaggtttac accaaacagt
    36121 atacttagtt acacttactg ctaactcttt catatttcta tcatatatag ttcctctttt
    36181 gggttctatt ggtatatctc ttgtttgttg ttctagtgct tttgtactca accaatttcc
    36241 cttcataagt tgtagatacc cagtcctaat aacaaggcaa aaaaacaatg cacatgctag
    36301 aataagaacg agtaccagcc ttttcttact tatcctcttt acttttctca aatctattcc
    36361 ccctagttat ccacatctat atactttatt tgtccatctt ttggatagtc catattgagc
    36421 aattccttag cctttagctc aatttctttt attgatgttt tttcagtttc ttttgccttt
    36481 agttcctcta atactatctc tttttttctt aaatcttttt gtaaataata gatatcgtac
    36541 ttcatttccg agatttttga ataaccacat agattcaata ttattactga aaaagttgca
    36601 atcagtaaaa tgcatttagc tcctattttt ttcttatttc tctttttata attattcttt
    36661 tttttatttt tatattcatt tagattatgt attttataat tttttttcaa attctcaccc
    36721 cgatagtata tagtttaaat tttcctctat actatatttg ttattaatca tatttcttcc
    36781 tctaaaattt catcgctaaa ttaatacact attacacaat ctaattgtat ttttttctat
    36841 acacaacaaa aacctataaa ttcacttata cttttatatg aatctatagg ttagatatta
    36901 atttctattt atttttatca cttctagtat ttttagataa tttcttccat aaatataata
    36961 tgtaactaac tatctactct ctgattccta agttctccat tatattactt aataccatct
    37021 atatctatat ttttatatct aaattttcat tatatatctc aataacaaag aacatttctt
    37081 atagtgttaa ttgtttttta attgctttac cagtgcaaca aaatatataa tactttttaa
    37141 ttaattataa taatcttata tataagatta ttctttagcc ttataactac ttatattttt
    37201 tctgctactc ttagcttagc acttcttgaa cgtggattta cttctatttc ttcctcacta
    37261 ggtaatatag gttttctagt tattattttc acctcagatt ctttatcaca ttggcatata
    37321 ggtaaatgct gtggacatat acaatctgat gctaaatgct taaatgcatt tttcactatt
    37381 ctatcttcta atgaatggaa tgttattata catattcttc caccttcatt cattatagaa
    37441 gaagcatcat ttatcatctt tgtaattact ccaagttcat tatttacttc tattcgtatt
    37501 gcctgaaaag ttcttttagc tgggtgaggt ccatctattc tggctttctt agggatagct
    37561 tttttaatta catctactaa ttctcctgta gtttctattg gtttgtttgc tctttcttca
    37621 actataaatt tagctatacg cttagcccag ttatcttctc cataatcttt tattatctta
    37681 gttaattcat cttctgtata agtatttaca acatcatatg cagaaaactc acacctaaca
    37741 tccattctca tatcaagtgg tgcatcttgc atataagaaa atcctctatc agcttcatca
    37801 agttgatgag atgaaactcc taaatcagct aatacaccat caattttgta aactcctatt
    37861 ttttctaatt cctcttttat attttcaaaa ttgctgtgaa caaacttcac tctactttca
    37921 tattcactca atctctcttt tgctgtgctt atagcatttt tatcttggtc aaaccctata
    37981 aataatccct tatctgacag tttttttact atttctttag aatgtcctgc tcctcccata
    38041 gtacagtcaa catacacgcc atctggcttt atatttaaat tctctataca ctcatttaaa
    38101 agaactgata catgatgaaa ttccatagtt acctccctta aattcacttt ttaaactatt
    38161 cttcactaat attattttta tttttctttg ataaataaac atgtgcaatt atgccaccaa
    38221 taactccaat tagacttata acttgtgcca ttctaagtgg tcctaacatt aaactatctg
    38281 ttcttaatcc ctctataaaa aatcttccta ttgaatataa agttatataa gtaactataa
    38341 cttgaccttc atatttttta ttttttctaa atagtaataa aactataaat attccaaaat
    38401 cccatataga ctcatataaa aaagttggat gtacctttac tccatcaacc attattcccc
    38461 aaggtaaatt ggtagcacct ccatgtgctt ctccattaat aaaatttccc catcttccaa
    38521 tagcttgtgc taaaggcatt ccaagtataa ctgtatcagc catttttaag aagttaatat
    38581 ttttaatctt agtgtatata taaccagcta atattccacc aattaaccct ccatgtatag
    38641 caagtccacc acctctaaaa ttaaaaattt gagacatatt ttgagcatag taactccaat
    38701 taaatacaac ataatagatt cttgcacaga ttagtccaac tggaattgct attatagcta
    38761 tatttaatac atcatcttct ttaattccca ctctttttgc ttcttttatt gctattaaag
    38821 ttcctaaaat cattccacat gccataagta ttccatacca catgatgtct atgccaaaca
    38881 gtgtaaatgc tactctatcc ataacgtcac ctctttgtat taattgaaaa tatttccaaa
    38941 gcaatatgct ataattacaa ttaattgtaa ttatactaaa ttatgataac acattaatgt
    39001 taattctaca aaaaatatta aaatttattc tacaactccc tacactatat tatatctaat
    39061 tactataaaa ttttttaaac taaatctctc atgactaaag ttacaagtgt acatagatat
    39121 tttttaatca aaaagattga tgcatatata catcaacctc ttctaattct tatctatagc
    39181 tcttaaattt ttacctcaat ttatataaat atattttata aatttgtttt accaaaataa
    39241 acatttaaaa acataaacta atacttaatt agttattctt attatgatta agtttctatt
    39301 taggctctac aatagaaata acacaatact cttctttaaa atgctctttt aatctttctt
    39361 caacttcctc aaaagtaata tctttaataa tatctaaata atccaataga tttatatcct
    39421 taaaaacata agatataaaa ctattaccta taaagtttat agaatcaaag cacttaataa
    39481 atgaccctat ttttttcttt ttagttctct caaactcttc ttttgataat ccttcttttt
    39541 ttgatttttc tatataatca agaattattt cttttacttt tttaggctct ttagaatctc
    39601 cagctattat gctaaaagca taatccactt gagacgaaaa tccagctcca aaattttcat
    39661 ttattaaacc ttgcatatac aaatcttcat aaagcttact tcctttttta aataacatcc
    39721 ctacaagtat atcagttaca atctctttac gtaaaagttc tttccctttt aaaccaacat
    39781 tgctatcttt aaaacctatg ttgaacatag gcatagatat agggaatttt tctataacct
    39841 ctttttcttt tacactttct ggttcttctg gataaaatct ttctatactc ttagagagtc
    39901 tgtctacttt gtaattgttt gactttttag ttacatctat aactttttct acatctaagt
    39961 caccaacaac aaataaagcc atatttcctg gattatagaa agtattataa catttatata
    40021 gctcttcctt agttatctta tatatactat caacagtacc tgctatatca attctagcag
    40081 gataatttac atacattgcc ttcaagcaat taaagtatac attccaatca gggtcatcat
    40141 tgtacatttt tatctcttgt gctattatac ctttttcctt ttcaacattt tcatctgtaa
    40201 aataaggtgt ttgaacatag tcaattagat gttctaaact ctcataaaaa ttttcagtag
    40261 cagaaaataa gtaggctgtc attgtaaaat tagtaaatgc atttgcattt actcctaatt
    40321 ttgaaaattt atcaaatgca tctcctccat caggttgctc aaacatctta tgctctagaa
    40381 aatgtgcaat accttcattc actctaattt tcttatcttc tccaattggt acaaattcta
    40441 agtcatttga accataatta gtagccaaaa tagcatactt tttcataaaa ccccttttag
    40501 gcataaaata tacatctaat ccattttgca atttctcata ataaacttct tctttcaata
    40561 tatcattaac tattttttcc atagacatct cctccattaa tttctcaaga agtatacagt
    40621 atcaagctct atatttttta tagcttcaac tatatctttt tttgtaacct tttctatact
    40681 agatattatg tcttgtactt cagaatttgt ctttgccata gactgagaga aaacaaaatc
    40741 agacatacca cctatattat cagttataga tttcatagaa ttaactaaag caagtttact
    40801 gttttctaat tcttcatcag atatatcacc tgcttttaca ctttctactt gtttttttat
    40861 caattctaca gctttatcat aatctttagt ttctatacca gatgatataa acatgatact
    40921 tttatacttc tctattgaag agaatatata gtaacataaa ctttcttttt ctcttacatt
    40981 aacaaataat ttagagtgtg gaccaccacc taagacattg ctcccaacta ctaaagcata
    41041 atatttatct atgtctgcat aatccacatt tgctctataa cccataacta acttaccttg
    41101 agttatttcc atttgttcat ctataacttt tacctcatcc acatttttta taaaatctgc
    41161 tcttggtata tttataattt cttctctttc aaattttaga tttttactta ttatatctac
    41221 aactttatct tcatcaaaat taccttctac aactatatct ataggtgatg ttttaagtat
    41281 gtttttataa tgttcatata actctcttga agttatacta tctatttcat cttcataacc
    41341 aaattcactt acactatatc tctctccttt acacatagcc tcaatacatt tatcttgcgc
    41401 atattccttt ttatcattta ttattgattg tatcttttct cttaaattct ctttttcaat
    41461 atttaaatat tcttctttaa acccaccatc tacaacaagc gtattattta ttacttcatt
    41521 aaaaaattct actacttctt caaatatact ctcatctaaa tacttttcac ttatgttaat
    41581 tactttaaaa ctcaatactt gacgttctcc acgttttaca gcatcagctc ccatagatga
    41641 accatataaa tcatctaatt tattggatat tgctctagca ctaggatatt tagcgcttcc
    41701 acttgctatt attgatggta ttagagcatt ttttgtagtt tctttttcat ccaatagtct
    41761 ctgaacatat aaacttataa gattcgattt aaacttttct gtttttatca aagtaagatt
    41821 aatattatta cctaaattta ttattttttt agtattaacc atatgtacct cctattttta
    41881 tttaaacatc aattttaaaa ttgaaattta tttatttttt aattacatat act
//
LOCUS       DAMJZE010000017        40839 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJZE010000017 DAMJZE010000000
VERSION     DAMJZE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.017"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            155..814
                     /gene="cprR"
                     /locus_tag="PC541_000803"
     CDS             155..814
                     /gene="cprR"
                     /locus_tag="PC541_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2838277.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1025..2083
                     /locus_tag="PC541_000804"
     CDS             1025..2083
                     /locus_tag="PC541_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2838278.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2334..4478
                     /locus_tag="PC541_000805"
     CDS             2334..4478
                     /locus_tag="PC541_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2748..2750,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDF2838279.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4527..5360
                     /gene="fdhD"
                     /locus_tag="PC541_000806"
     CDS             4527..5360
                     /gene="fdhD"
                     /locus_tag="PC541_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2838280.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5371..5544
                     /locus_tag="PC541_000807"
     CDS             5371..5544
                     /locus_tag="PC541_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838281.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5602..6153
                     /locus_tag="PC541_000808"
     CDS             5602..6153
                     /locus_tag="PC541_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2838282.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6178..7554
                     /locus_tag="PC541_000809"
     CDS             6178..7554
                     /locus_tag="PC541_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2838283.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7554..8069
                     /locus_tag="PC541_000810"
     CDS             7554..8069
                     /locus_tag="PC541_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2838284.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8147..9430)
                     /locus_tag="PC541_000811"
     CDS             complement(8147..9430)
                     /locus_tag="PC541_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2838285.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9618..10040
                     /locus_tag="PC541_000812"
     CDS             9618..10040
                     /locus_tag="PC541_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2838286.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10142..11080
                     /locus_tag="PC541_000813"
     CDS             10142..11080
                     /locus_tag="PC541_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2838287.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11266..13290
                     /gene="ligA"
                     /locus_tag="PC541_000814"
     CDS             11266..13290
                     /gene="ligA"
                     /locus_tag="PC541_000814"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2838288.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13450..14799
                     /gene="rph"
                     /locus_tag="PC541_000815"
     CDS             13450..14799
                     /gene="rph"
                     /locus_tag="PC541_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2838289.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14799..15269
                     /locus_tag="PC541_000816"
     CDS             14799..15269
                     /locus_tag="PC541_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2838290.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15463..16749
                     /locus_tag="PC541_000817"
     CDS             15463..16749
                     /locus_tag="PC541_000817"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2838291.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16899..17483
                     /gene="clpP"
                     /locus_tag="PC541_000818"
     CDS             16899..17483
                     /gene="clpP"
                     /locus_tag="PC541_000818"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2838292.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17502..18752
                     /gene="clpX"
                     /locus_tag="PC541_000819"
     CDS             17502..18752
                     /gene="clpX"
                     /locus_tag="PC541_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2838293.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18823..19350)
                     /locus_tag="PC541_000820"
     CDS             complement(18823..19350)
                     /locus_tag="PC541_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2838294.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19338..19850)
                     /locus_tag="PC541_000821"
     CDS             complement(19338..19850)
                     /locus_tag="PC541_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2838295.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            20030..22393
                     /gene="lon"
                     /locus_tag="PC541_000822"
     CDS             20030..22393
                     /gene="lon"
                     /locus_tag="PC541_000822"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2838296.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDSLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22383..22994
                     /locus_tag="PC541_000823"
     CDS             22383..22994
                     /locus_tag="PC541_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2838297.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23259..24629)
                     /locus_tag="PC541_000824"
     CDS             complement(23259..24629)
                     /locus_tag="PC541_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2838298.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24804..24919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25207..26286
                     /locus_tag="PC541_000825"
     CDS             25207..26286
                     /locus_tag="PC541_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2838299.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26677..27357
                     /locus_tag="PC541_000826"
     CDS             26677..27357
                     /locus_tag="PC541_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2838300.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27350..28729
                     /locus_tag="PC541_000827"
     CDS             27350..28729
                     /locus_tag="PC541_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2838301.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28900..29985
                     /locus_tag="PC541_000828"
     CDS             28900..29985
                     /locus_tag="PC541_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2838302.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30039..30398
                     /locus_tag="PC541_000829"
     CDS             30039..30398
                     /locus_tag="PC541_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2838303.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30451..31323
                     /locus_tag="PC541_000830"
     CDS             30451..31323
                     /locus_tag="PC541_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2838304.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31324..31767
                     /locus_tag="PC541_000831"
     CDS             31324..31767
                     /locus_tag="PC541_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838305.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31782..32366
                     /locus_tag="PC541_000832"
     CDS             31782..32366
                     /locus_tag="PC541_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838306.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32619..33656
                     /locus_tag="PC541_000833"
     CDS             32619..33656
                     /locus_tag="PC541_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2838307.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33737..34102
                     /locus_tag="PC541_000834"
     CDS             33737..34102
                     /locus_tag="PC541_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838308.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34107..34424
                     /locus_tag="PC541_000835"
     CDS             34107..34424
                     /locus_tag="PC541_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838309.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34472..36076
                     /locus_tag="PC541_000836"
     CDS             34472..36076
                     /locus_tag="PC541_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838310.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36200..37828
                     /locus_tag="PC541_000837"
     CDS             36200..37828
                     /locus_tag="PC541_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2838311.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38093..39442
                     /locus_tag="PC541_000838"
     CDS             38093..39442
                     /locus_tag="PC541_000838"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2838312.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39598..40677
                     /locus_tag="PC541_000839"
     CDS             39598..40677
                     /locus_tag="PC541_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2838313.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16783 a         4306 c         7346 g        12404 t
ORIGIN      
        1 tataaagttt ttgtatctaa gatattttat ttgtaaaata gtaattaatt attgtaagct
       61 ataatatgag agtattatgg ttaatgtgaa ttttaaagat attattaaat atataaatta
      121 gaatactatt aaacggataa atggaggagt tattatgtct aagatattag tagtagatga
      181 tgagttagat atgttaaagt tgattgaaaa tgtgttgaaa agagatggac atgaagtaaa
      241 ggtaattagt gatgtagaaa aagctatgaa catggatttt aattatttta atctaataat
      301 attagatgtt atgatgccta agatggatgg ttttgaagta tgtaaaacta taagaaataa
      361 ggttgattgt ccaatactgt ttttgacagc aaaaaatatg gaaagtgata ttatgtacgg
      421 tcttggtata ggtgcagatg actatataac caaacctttt ggtgtaggag aattaagggc
      481 taggataact gctcatttaa ggcgagaaag tagagaaaaa aagaatttat taactatctc
      541 aaatgttcaa tttaactttt taggcaaaga agtttcaatt gatggtaaaa aaattaattt
      601 tactaaaagt gaatatctaa tatgtgaatt tctagcaaaa aataagggac aagtattttc
      661 aaaagaaaga atatatgagt cggtatatgg ttttgatgga gaaagtgata taagtactat
      721 cacagagcat ataaaaaata tacgaagcaa attaaaacaa tatgatattt atacaataga
      781 aactgtatgg gggatagggt ataaatgggt gtaataagta taagtgttgt ttagtgaaaa
      841 tattatatat atctacaaaa tgtttgtaaa atattaatct atatattaaa atttaattta
      901 tagattaata ttttttgtaa atgacaaaat tgtaaggtat tttgttataa aatattaaga
      961 ttcatagtgt atactaataa tatagtattt agtataaatc ttgagcaaag gatggttatt
     1021 tctaatggat agactaaaga atattagaaa gagtttacta cagtatacat ttttattttt
     1081 gttaataggt ataacaattt acctagtgtt taaaacattg gatattaaaa tgctatcaaa
     1141 tgttatggtt atggtagata agaagttttt attcataggt gctttggcaa taatgtgtca
     1201 cataatgctt gaaggagtag taatgaagat aattatagaa agcactcaca aagttaatat
     1261 tagatttatt ggttttaagc tagccattat gggattttat tataatttaa taactccttt
     1321 tgcttcagga agccaaccag tacagatata tgtacttaaa aaatgtaaaa tgccacttag
     1381 taaggcaagt gcagttgtta ccaataagtc tattatcttc caaatagtag ttacttttta
     1441 ttgtacaata cttgttttaa tgaattttgc tatgttagaa aaacaaatga aaatgattat
     1501 gcctctagtg tttctaggga tagccattaa ttcatttgtc ttaattatga taatattagt
     1561 tattttaaat cctgaaaaaa taaaatattt tatgagaatt actataaagc atttatcaaa
     1621 aattaagttt ttaaaatttt tagataataa gattgaatcc atagaaagtt ttatagatga
     1681 ttatagtaaa gcaataaatt tttttgcaaa gaataaaaag gtattaattt caactataat
     1741 agtaactttt attcaattaa gtgcttactt tagtgtttca ttttggatat ataaagcatt
     1801 taatttacaa ggttatagct atatttatat actgacatta caagcatttt tatatatggc
     1861 aatatctcca ataccaacac ctgggaatgt aggagctaat gaattagcct ttttcactat
     1921 attcaaatca gtattccctc aacctttgat gggatatgca gtatttttat atggtggatt
     1981 tatgtactat ttgattctga ttggaagtgg aatatttact gtaattactc actatagaat
     2041 gaaaaacaga attggcagaa atattgtttt aagtgaaaac taaataagtt taaagaatat
     2101 atattgagta tattaaaaaa tatgtcataa gaactagaat attttaatta aatggttgaa
     2161 atataaagat tattatgaca tatttttttt gaaatttaag aggaattgtg agaaaattgt
     2221 tgaatttaat agatagtatt gcaaaatatt tatgattttg catattataa aaacactcat
     2281 tatctttaag ataaatttta cttatcaggg gataaatcta aggggggaat tgtatggaga
     2341 aaaaaatttt aacagtttgt ccatactgtg gagctggatg ccaattatat ctagtagtaa
     2401 aagataataa gatattaaga gctgaaccgg ctaatggtag aacaaatcaa gaaagtttgt
     2461 gtttaaaagg tcgttatgga tgggattttc taaatgaccc taaaattcta acacctaggc
     2521 taaaaaagcc aatgataaga aaacaaggtg tactagaaga ggttgaatgg gatgaggcta
     2581 taaactatac agcttcaaga ctaaatgaaa ttaaagaaaa atatggacct gattctatta
     2641 tgggaacagg ttctgcaaga ggacctggta atgaagcaaa ctatataatg caaaaattta
     2701 tgagagctac aataggaact aataatgttg accattgtgc tcgtgtctga catgcaccat
     2761 ctgtagccgg tctggcttac tcattaggta gtggtgcaat gtctaattct ataccagaga
     2821 tagaaaatgc agatgtttta tttatatttg gatataatgg agcagattca catccaatag
     2881 tggcaaatag aatagtaaaa gctaaaaaaa atggtgctaa attaattgtt actgacccta
     2941 gagtaactga atctgcaaga attgctgata tacatctacc tataaaaggt ggaacaaaca
     3001 tggttttagt aaatgctttt ggaaatgtat taatagaaga aggtctttac aataaagaat
     3061 ttgttcaaaa tcatacacaa ggatttgatg agtataaaga aattgttaaa ccatatacag
     3121 ctaaatatgc tgaaaagatt acaggaatcc cagaagagct tataagaaaa gctatgagag
     3181 aatatgcaaa aggtaaaaaa gctatgatac tttatggaat gggtgtctgt caatttggtc
     3241 aagctgttga tgtagttaag ggacttgcat caattgctct acttacaggt aattttggta
     3301 gagaaagcgt tggaatagga cctgtacgtg gacaaaataa tgttcaagga gcttgtgata
     3361 tgggagctct tcctaatgta tatcctggct atcaaaatgt aactgatgat aagataagag
     3421 aaaagtttga aaaagcttgg ggggtaaaat tatctcctaa taatggttat agtttaactc
     3481 aagttccaaa tttagtacta aaagagaaaa agcttaaagc ttattatata tttggagaag
     3541 acccagtgca aagtgaccca gatgcatcag aagttagaga agcattagat gaattggaat
     3601 ttgtcattgt tcaagatata tttatgaata agacagcact tcatgctgat gttatacttc
     3661 cagctacttc ttggggagaa catgagggag tatatacatg tgcagataga ggattccaat
     3721 tgatgagaaa agctatagag ccacaaggag atgtaaaacc agactggcaa attataagcg
     3781 aaatttctac agcaatggga tatcctatga attataaaaa tactaaggaa atatgggacg
     3841 aacttagaca attatgtcca agtttcttag gtgctactta tgaaaagata gaaactcaag
     3901 gatgcgttca atggccttgt aagagtgaaa gtatggaaga taaaggaact atgtatctgt
     3961 atgaaggtca aaagttttct actcctaatg gtaaaggtaa tttgttcgct gctgaatgga
     4021 gaccccctat ggaagttgaa gatgatgaat acccatttag tttatgtact gtaagagaag
     4081 ttggacacta ttcagtaaga acaatgactg gtaattgtag aacacttagt tcattagaag
     4141 atgaaccagg acgtgttcaa ataaattcta atgatgctga aaaattagga attgaagatg
     4201 atgaattagt tagaattagt tcaagaagag gaagtgtaat aacaagagca acagttactg
     4261 atagagttaa ggaaggtgca acatatatga cttatcagtg gtgggttgga gcttgcaatg
     4321 aacttacaat agcaaattta gaccctatat ctaaaacacc agagtataaa tattgtgctg
     4381 ttaaacttga aaaacttgaa gaccaagaat tagcagaaaa atgtgtaaga gaagagtatc
     4441 aaagtttgaa agacaaaatg acagctacaa atatctaatg atttattaga tttaagattt
     4501 ataaaaggca gaaaggctaa tattttatga acaatatata ctcaaaaact aaattacaag
     4561 aaaatagtaa ggataaagtt tttgaaaata gttatagtct agtggactac aaatatttaa
     4621 attcttttaa tgtagaagta agcaaaataa atgaacatga agtatcaata gataatgaag
     4681 aaataattgc agaatatcca cttagtttta ttttaaatga taaatatgta aatacatttt
     4741 tatgtacccc atataattta gaggaactta tagtaggatt cttgaaaaca aaaggataca
     4801 tagaaaacaa aaaaagctta ttaaaatttg agataaatga gagtgaacgt tttgcaaaag
     4861 taactattca agagaaacaa aaaaatcaag atgagcaaat tatattttta aattcactag
     4921 actatataac atgtgctcct gtaaagagca atataaaaat aaattatgat acgatttaca
     4981 gtataatgga taaaaattta aattcatcag aattatttaa aaatactggt ggggtacata
     5041 gcgtatctgt atttaatttt gataaaaaaa tagtcacttg tgaagatgta gcaaggcata
     5101 atgcaatgga taaggctatt ggattttgtg ttttaaatga cataacatta aaagataaaa
     5161 ttgtggctgt aagtggaaga atatcattgg aaatgatatt aaaagcagct atgactcaaa
     5221 ttcctattgt agtatcaaaa tcagcaccga ctaatttatc aatagagctt tctaaaaaat
     5281 taaatataac gcttattgga tttgtaagag gaagacggat gaatatttat gcaaatccac
     5341 agagagttat caaaaagtga gaatgaatat atgtgtacta aaaaagaaga gcaaagatat
     5401 tcaaagtgtg aaatctgtgg agtattaatt gatgatactg ataaggtgta taataacaca
     5461 tacaatttgt gcaaaaaatg taaacgaaat ataagtactg agaaaatagt gaaatatata
     5521 aatatatatg atgaaagtaa gtagacatat gatgaaaata actagagggt tggaaatata
     5581 caaattgaaa ggtgggataa tatgaaaaat aaaataggct cttttgtaat agcagaccca
     5641 aacaaatgta caggttgtag agcttgtgaa gtagcttgtt ttacaatgca taatcagcat
     5701 aataatgtgg gttatacagt tggaacagtt gatatacctg ttatacctag actttactta
     5761 gtaaaaggtg acaatttctg tatgccaata cagtgtagac attgtgaaga tgctccttgt
     5821 ttaaactcat gtccacaaaa agctatagtt aaagaaaaca atattatgtc tgtaaatgaa
     5881 gaaaagtgta taggatgtaa aacttgtctg ctagcatgtc cttttggagc tatagactta
     5941 cttccacaat atgaagatgg taaagaagta gaacaagtaa ttttagatga aaataagaag
     6001 atagcttata aatgtgactt atgtaaagac aatgaaaaaa tagcttgtat aagtgcatgt
     6061 cctcaggaag cattaaaact tgttactcca atagatgaca aaaaagctaa gaatagacaa
     6121 gctgcactta gtttattgtt aacaaataaa taaaggatac aggggtaggt gacgtttatg
     6181 ataataaata tagataagga tttatgtact ggatgtagag aatgttcaaa ggtctgtcca
     6241 gtaaatgcta ttgaaggtga agagggaaag ccacaagaga taaatttaga tagatgtgtt
     6301 atgtgcggtc aatgtgttca aacttgtaaa tcttatgcct cagttataga tgaaggattt
     6361 gagtttttac aagagaaaaa acaagaaaga gagataccag agtctataaa tgaacctata
     6421 tttgcagcat acaatgtatg tgatatagat aaggtgaaga aagctttaag tgacccaaat
     6481 gtattcacta tggtccaatg tgcaccagca gttagagttg ctttaggaga agattttgga
     6541 tattatttag gatacttagg tgctgggaaa atggcagcag cacttagagc tttaaatttt
     6601 gatagagtct acgatactaa ttttgcagca gatttaacta taatggaaga aggtagtgaa
     6661 ttaattaaaa gagtgacaga aggtggaact cttccaatgt ttacatcttg ttgtccagct
     6721 tgggtaaaat tcatggaaaa aaattatcct aaattaacta atcatttatc ttcttgtaaa
     6781 tcacctcagc agatgggtgg tgcaatattt aaaacttatg gtgcagagat aaataatgta
     6841 gatgcatcaa agatatttag tgtttctata atgccatgta catgtaaaaa atatgaatgt
     6901 gatagagaag aaatggattc tagtggatat agagatgtag atgtagtatt aactactaga
     6961 gaactagctt atttaataaa agacatggga attgatttta aaaatttaag agaagaaaaa
     7021 tttgatagtc ctttaggtag ttatagtgga gctggaacta tctttggtgt tactggtggg
     7081 gttatggaag ctgctataag aacaggatat gagcttataa ctggagaaag tattcctgat
     7141 gtagaagtaa aacaagtaag aggagaaaat ggttttagaa agtcaactat taaaatagga
     7201 gacttagact taagagttgg agtagtaagt ggattaaaaa atgtaatacc agttcttgaa
     7261 gacttggaaa aaggaaaatt agatgtagat tttattgaag ttatgacttg tcctgttggg
     7321 tgtgtaagtg gtggaggtca accaaaaatt ttactagaag aatataagga attggcttat
     7381 gaaaatagaa caaaagcaac atacgtgcat gatgaacatt tagcaattag aaaatctcat
     7441 gagaatcctg atattaaaaa actgtatgat gaatatctgg tagaaccatt aggagaaaaa
     7501 tctcatcatc tattacacac tacatattgt ataggaaagg aagtggctaa gtgatgaact
     7561 attttgttat agcagaccct gataaatgca taggatgtag aacttgtatg ataggatgtg
     7621 tagttgcaca ttcagatgaa gatatttttt accaaaatcc agatgaaata aattttaatc
     7681 caaaactttc tgtcataaag acaaaagaag taagtgctcc aatacagtgt agacattgtg
     7741 aagatgctcc ttgtgcaaag gcatgtccaa atggtggtat agttagggtt ggtaacacaa
     7801 taaaaataaa tgaagaaaat tgtataggat gtaagacttg tatgttagca tgtcctatag
     7861 gtgcaataga catagttaca ttaaaagatg tagatgaagg aaagctttgc tttagagaaa
     7921 gaatgacagc aaataagtgt gatttttgta tagaatcgcc agaagggcct gcatgtgtaa
     7981 atgtttgtcc aactaaggca tttactatag ttaaggatga ggatatagat agtaaagtta
     8041 aaaataagag aaaattagct atattgtaat ataaaaaaga tggagtttta tttatgatta
     8101 aaagctccat cttttttatt ataatattta tatatctaaa aatcaattat tgcattagtt
     8161 tatctaatct ttctttattc aattttttca ttcctataga agttataata agtaaagtat
     8221 aggcaattat aactgataaa attataatag accaagaaaa tctgtatcca aaattatcaa
     8281 ctaacaatcc aaataatgtt gaacctaatg caaatcccac tgcaaaaatt aattgtacga
     8341 ttccatatat gctaccaaac tccttttgac caaagaaaga gccagttaaa taagcaggac
     8401 caatcatata agcaaataca gaaagacctt tcaaaatggc aaatacaaat gcaaaagtag
     8461 aagatttatc taccaatagt aatgataaag ttgctataag aaccaataca aaagatataa
     8521 taagtggctt tgtttgaccg aatttatcaa acaataatcc tccaaataaa ttaccaatga
     8581 gagcaaataa ggcaaatgtg gagcctactg ttcctattag aataggattt agataatttc
     8641 taagataagt tggatattgt acagataatg atgaaacata tataccaaca aatataaatc
     8701 caatagtcat aatccaaaaa tatttaattt ttttagcttc ttttaatgta taaccccata
     8761 ctgaaggaga aattgtatca gatgctttct cttttttacc tttagatttt accttttcat
     8821 taatatcttt aggcatcctg agtatgaata atattacagg tataccaact attaatgcta
     8881 taattccaaa tattagataa gaacgactgt atccttgagt tgctaataag cgaactgcaa
     8941 gtggttgtag aaatatacta ccaatagaac caccagcaaa agcaaagccc aaagcttttc
     9001 ctttgctttc ttcatcaaac caagcactta ttaaaacagg tattcctata gcagatataa
     9061 ttgtagcgcc aatttgaaca atacatgata cagtgtaaaa ttgccatagt tcagtacata
     9121 tagaaaaagt agcaaaagca atactagaaa ttatacaacc taataaatat agtatcttaa
     9181 tattgacttt agaaaataat acacctataa aaggtgatgc aattgctgag gcaatagtac
     9241 caatagtgaa tattagagaa aaagaagcta aactaaagcc ttctccttct acaacataac
     9301 ttataaattg aggctgtata cttgctacaa tactaaatgg aacagcttgt attaacatac
     9361 aaccaagtac tataacccaa gcatttgaaa agtttttaat gctaaattta ttgtttagta
     9421 atgtattcat ggtatctctc ctttaggtta tgatttgtag atattaatct aattattcta
     9481 attttatata ttagttttgg atagcctaac tatttaaata taatttgggt attaatataa
     9541 aaattaacta taaaaattga ttattttaaa tgtatggggt aaattaaagt atataaataa
     9601 atgaaaggat ggataaaatg tcatttgaaa aaataaataa taataaccta gataaaatac
     9661 acaataggaa gtgtattatg cttgtaaact ttaataaaaa agaattatca ttaattaaaa
     9721 atgtaagtaa ctttgctgga ataagagacc atatcatctt aagtgataaa aatggagaga
     9781 atataataag agacatttta gatggaaata tatcaaatga atgtgaaaat gggattaaga
     9841 gtaagtctgt aatatttaat aacactccaa gtacacaagt aaatgcattt ttagaagggc
     9901 taaaaaaagt gagaataaat agacctttta cagctatggt aacagagaca tctattgatt
     9961 ggacattgaa taatttaata gaaaatttag ttcaagaacg taatgctatg tctttaggaa
    10021 aaacaattga acataagtaa atttaagata tcttatagta attttaacta atgtacattg
    10081 agtatcatat tatattttag aatagaaaat tttaaatgtg ggtagatgga ggagatttga
    10141 gatgaaagtt ttatttacaa taaattatgg aaaagaaaaa tttgataaaa taagagaatt
    10201 aggctatgat gtcatacatt actctgaaga tagtattgaa aataatgagg atgtaaatac
    10261 agcagatgta ctagttacat acaatccatt tgagagatta gatatttcta agatgaaaaa
    10321 tttacaatat atacagacaa ctagtgtagg tatagaccaa cttccaaaag aagaggtatt
    10381 gaagagaaat attaagatag ctaataatag aggcggatat agtgtaccta ttggtgaaag
    10441 tattgtattg tatattttag aaatatataa aaatagtgca aaatttttta aacaacaaca
    10501 aaataaagaa tggaagatgg atttttctgt atcagaattg tatggacaaa gagttggatt
    10561 tttaggaaca ggtactatat cttcagaagc agcaaaaaga ttaaaagctt ttggtgtaga
    10621 aatatggggt gtcaatactg atggaagtag taaaaaatac tttgataagt gttttgctac
    10681 tgaggatatg aatattgtat ttgaagaatg tgatatagta gttgtagcaa tgccttctac
    10741 aaaaagtaca gatggaatag taaacaaaga tatgtttaaa cttatgaaag atggttctgt
    10801 atttataaat gtaggtagag gaaatacagt taatcagaaa gatttagaag aatgtgtaga
    10861 gaaatttaga ggggtagcat tagacgtatt tgaaagtgaa cctttaaaca aggacaataa
    10921 gttgtgggag tgcgaaaatg tcatagtaac tcctcataat tcttgggtgt cagataaaaa
    10981 caaagagaga acatttaata tggtttataa taatttgaaa cactatataa tggagaataa
    11041 acctgtagat aatgttgtag acatatcaag aggctattaa aaataaaaaa ttttgataaa
    11101 aataaaaact cttattagtc gtattaaaga tactaataag agtttttttt gtaataaatt
    11161 taaagcccaa aaagtgatat actaaaaaat atataaatag gagataatta attataggga
    11221 aatttcaaaa taatttaatt ttaaaaatat gaagaggtgc aaaaagtgag cgtaaaagat
    11281 gaaattaata aacttataga gacaataaac taccataatg aaaggtatta taatcaagat
    11341 agcccagaaa tatcagatta tgaatacgat aagttaatga aggagttaat ttctttagaa
    11401 gaagaacatc cagaactaaa gagaattgat tctccaacaa atagggtagg aggtaagcca
    11461 ttagataagt tcaatcaaat tgtacataaa actcctatgt tgagtttatc aaatgctttt
    11521 tcagagcaag atttgagaga ttttgataag agagttcaag aatatgtggg agaaaatgtt
    11581 gaatatgttg ttgaatttaa aatagatggt ctttctgtag gtctaactta taacaatgga
    11641 gagtttgaaa aaggagcaac tagaggagat ggagttgtag gagaagatat ttctcaaaac
    11701 ttaatgacag taaaaagcat acctcttaag ataaatgata aaaaagaact aatagttaga
    11761 ggagaagtat atatatcaaa agataatttt agaaaagtaa atgaacaaca agaagaacaa
    11821 gaacagccat tatttgctaa tccaaggaat cttgcagctg gttcattgag acaattagac
    11881 ccaaaactaa cagcaaagag accattagat atatttgtat ttaatatgga aaatatagaa
    11941 gaatacgatt tagaaagtca cagtgaatca ctagagtact tagaaaaact aggattttca
    12001 gtaagtcaga gttataaagt ttgtaaaagt atagatgaag ttattgagca tattgaatac
    12061 tggacagata acaggggtaa tttagactac gaaatagatg gtatggtaat aaaagtaaac
    12121 aatataaaac aaagagacga aatgggatat acagccaaaa gtcctagatg ggcaattgca
    12181 tataaattcc cagcagaaaa gaagaaaact aaaatagtag atataatagt agaagttggg
    12241 agaactggta caataacacc ttcagcaatt cttgaaccag tcagacttgc aggtaccact
    12301 gtaagtagag ctacacttca caatgaagat tatataagag aaaaagatat aaaaataaat
    12361 gatacagtat tagttcaaaa agcaggggac ataatacctc aagttttaga agttataaaa
    12421 gaagaacgta ctggagatga aatagatttt aaaatgccag aaaagtgtcc agtttgttca
    12481 gagccaacaa taagattaga aggagaagct gctgtaaaat gtatcaatat gtcttgccca
    12541 gcccaaataa gaaggggaat tatacacttt gtgtcaaggg atgcaatgaa tatagatgga
    12601 ttgggagaat ctataattac actgctatta aatgaaaaaa ttattcaaga tgtatctgat
    12661 ttatattata taaaaaagga agatgtagtc gacctagaga gaatgggcga aaaatctgct
    12721 gacaatttaa taaattcaat tgaaaaatca aaagaaaatg atttatggag acttataaat
    12781 ggattaggta ttaaatttat aggagttaaa gcagctaaga tacttgcata taactttaag
    12841 gatttagatg aagtaatttc tgcaactagt gagcaactag aagaattaga ggaatttgga
    12901 agtataatgg caaacagcgt tgtcgagttt tttaaggaag acaaaaatat gaacgttata
    12961 aacaagttaa aaaatgtagg agtaaataca aaatcattgg ataatacagg tgaaaaagta
    13021 gaaaaaatat ttgaaggtat gaaaatcgta ttgactggta cacttcctac attaaaaaga
    13081 aatgatgcaa aagatatgat agaaaaaaga ggtggaaaag ctacatctag tgttagtaaa
    13141 tcgacaacat ttgttttggc aggtgaagaa gctggttcta aacttactaa agcaaatgat
    13201 ttaggaatta aagttataga tgaagagaga tttttagaat tattaaagtt atcatcgaaa
    13261 gatgaagtaa aagctgtatt agagagttaa aaagtcgctt aatttagtag aaaactacta
    13321 taattaatga tagaatagtt cagtaagcta ataagattac gaaaaatgac aataattaat
    13381 atttgacaaa attaaaaatt ataaaaataa atttataaaa aataaaaaat atgagaaagg
    13441 atataaaaaa tgaccagaag ttatgataga agtaatgaac agataagacc tgtaaagata
    13501 actagagatt atacaaagta tgcagaaggt tctgttctta tagaaatggg agagacaaag
    13561 gttatatgca ctgcatcagt ggaagaaaaa gttcctcctt ttttaagaaa tagtggtact
    13621 ggatggataa atgctgaata ttctatgcta ccaagagcaa cacaacaaag aaagataaga
    13681 gatgcatcta ggggaaaaat tgatggaaga acacaagaaa tacagaggtt aataggaaga
    13741 acaataaggt ctgttgtaga cttgaataaa ttaggagaaa gaactatttg ggtagattgt
    13801 gatgtgattc aggcagatgg tggaactaga actgcatcta taacaggggc atttgtagca
    13861 gttgcagaag caatatataa attatataaa gcaaagacaa taaaagagat gccaataaca
    13921 aattttgtgt ctgcaattag tgttggtatt gtaaatggaa tacacatgct agatttatgt
    13981 tatgaagaag attctaaagc acaggtagat atgaatataa taatgactga taagtgtgag
    14041 tttgtagaag tacaggggac aggagaagag aaaccttttt ctagagcaga tttaaattta
    14101 ttgttagagt taggtgaaaa agggaataaa gaattaataa ggcttcaaag agaagcttta
    14161 ggtgaaatat cagatgagat attgggtatg gattatggta atgaagtagt gattgctact
    14221 aataatgctc ataagctaga agaaataggt gaaatcttaa aagattttga atataaagtg
    14281 tactctttaa aagatgttga tttagcaggt atagagatag tagaagatgg aaaaacattt
    14341 gagcataatg cgttgataaa agctagaaca atagcaaaaa agactaaact tatagcaata
    14401 tcagatgatt ctggcttaga agtagatgct ttaggcaaaa aacctggtgt ttattcagct
    14461 agatatgcag gagaacatgc aacagatgaa gaaaatagaa aaaaactgtt aaaagctatg
    14521 caaaatgtac caatgagcaa aagaaatgca agattcgtct ctgcaatagc agtagttttt
    14581 cctgatggaa aagaatttgt agtaagagga atttgtgaag gaatgatagg atttgaagaa
    14641 aaaggtaaaa atggttttgg atatgatagc ttatttatag taaaaggata tgataagact
    14701 tttggtgaaa tacctagtgt tataaaaaat tcaatcagtc atagagcaaa cgcactaaaa
    14761 cttatgaaac aagagtttat aaaaagggta gtgaagtaat gaaaataggc atagtaagcg
    14821 acacacatat gatgattaaa aatatggaaa aaacaatacc atatttgaaa gaatgtgacc
    14881 taataattca tgcaggagat aactttactg attcaagata tattcatagc atgactaatg
    14941 taggtattat tgcagttaaa ggtaattgtg actttgatgc tgttgaggaa gaagttgtct
    15001 ttgaagtggc aaataaaacc atattcttat gccatggaga taagtatggt gtaaaatatg
    15061 gaactaatat gttagaaaaa aaggcaactg aagttgatgc tgatatagtt atatttggcc
    15121 atacccatac cccatttaga gaaattaaag atggtgtact ttacataaat cctggaagca
    15181 cttcacttcc aagaggagta tcgtataaaa gttttgtcat tatggatata gaagaagatg
    15241 acataaaaat tgaagaaata cgcatataaa ggtgtattta tctaaaaaaa tggcaaaaaa
    15301 cgctcaaaaa attaaatttt atcaaataag tacaacgata ttaatggaac acttgtaaat
    15361 aaaaaaactt atgtgatata attaagtggt tgaattataa atagaatgaa ttataatacc
    15421 gttatcgggt attacataaa tatcactagg aggattagaa ggatgaaagc agaactaatt
    15481 aaaaaagagg gtaataaagt tactttaaaa ataacagtag ataacaataa atttgaagca
    15541 gcagtaaata aggcttataa taaaagcaga aataaataca atatacctgg atttagaaaa
    15601 ggtaaagctc caagaattgt tatagagact caatacggaa aaggtatatt ctacaatgat
    15661 gctatagaaa tattattccc agaagtttat ccagaagcta tcaaggaatt agacatagac
    15721 ccaatagaca atccagattt agatatagaa gaaataagta aagacaatgg gttggttatg
    15781 gttcttaatg tagaagttaa accagagttt gaattaggaa actacaaggg tatagaaata
    15841 gctaaggttg agaatactgt aagtgacgaa aatgttgatg ctaaactaca agaaatggtt
    15901 gaaaaaaatg ccagattagt aagtgtagaa gacaaagctt tagaagatgg agatacagct
    15961 ataatagact tcgaaggttt tgaaaatggt gtagcttttg atggtggtaa aggtgaaaat
    16021 tataacttag ttataggttc aaacacattt ataccaggat ttgaagaaca attagtaggt
    16081 aaaaaagctg gtgaagaagt agaagttaat gttactttcc cagaagaata tcactcacaa
    16141 gatttagctg gaaaaccagt tgtatttaat gtaaaaataa atgatgtaaa agtaaaagaa
    16201 ttatctgcac ttgatgatga atttgctaaa gatactagtg aatttgattc tttagatgaa
    16261 ttaaaagcag atgtaagagc taagttagaa gaagaagcta aaaatagagc agatgcagaa
    16321 actagaaatt cagtagtaga aaaagttgct gaaaatacag aaatagaaat accagaagtt
    16381 atgatacaac atcaaataga taatatgtta aatgaactta actaccaatt acaatatcaa
    16441 ggatttggat tacaacaatt actagagatg actggtagaa ctatggaaga attaagagaa
    16501 gagagaaaag aagatgctaa aaaattagtt aagtcttctt tagtattaga agcaataact
    16561 aaagctgaag gaattgaagc aacagaagaa gaatttaagg ctgagttaga aaaaatggct
    16621 tctgcttaca atatggaagt agaaaaaata gaagcttctt taagagatgc tgacaaagaa
    16681 gatataaaag gtcaaataaa aataagaaaa acaatagatt tattagttga taatgcaaca
    16741 atagcttaat aaaataaacg ctaactaata tagttgacgt tatgaaatgt ttagtatata
    16801 atgatgtgca aatataattt agttaaatgc gtataataat ttacagtagc ttaaaaaaag
    16861 ctactgtaaa ttatatagaa taaagggggt atgaaaccat ggcattagta cctgtagttg
    16921 ttgaacaaac aggaagagga gaaagatctt atgatatttt ttctagacta ttaaaagaca
    16981 gaataatctt cttaggagat caagttaacg atgccacagc agggcttata gtagcacagc
    17041 tattattttt agaagctgaa gacccagaca aagatataca tttatatata aattctccag
    17101 gaggaagtat aacttctgga atggctatat atgatactat gcaatacatc aaaccagatg
    17161 taagtactat atgtataggt atggctgctt ctatgggagc attcttgttg gcagcaggag
    17221 caaaaggaaa aagactagca ctaccaaata gtgaaataat gatacatcaa ccattaggtg
    17281 gagctcaagg tcaagcaaca gatatagaaa ttcatgcaaa gagaatttta aaaataaaag
    17341 aaactttaaa tgagatatta tctgaaagaa ctggtcagcc attagaaaaa attaaaatgg
    17401 atactgaacg tgataacttc atgagtgcac tagaagccaa agaatatggt ttaatagatg
    17461 aagtatttac aaagagaccg tagatagaga gaggtgttaa tatgtctaaa tatgaagaaa
    17521 aacgacaatt aaagtgttca ttctgcggta aaaatcaaga tcaagtaaga agattaattg
    17581 caggtccaaa tgtatatatt tgtgacgagt gtgttgaact ttgtgacgaa attattcagg
    17641 aagaaataga agatactata gatgaagata ctacatcttt accaaaacca aaggaaatga
    17701 tggagattct aaatgattat gtaataggtc aagaaaaagc taaaaaagct ttatcagttg
    17761 cggtttataa tcattataag agaatataca gtaaaaaatc tagtagtaag gatatagaaa
    17821 ttcaaaagag taatatactt ttattaggac ctacaggttc tggaaagaca ttacttgctc
    17881 aaactctagc aagaactcta aatgtacctt ttgcaatggc agatgctaca tcattgactg
    17941 aagctggata tgttggagaa gacgttgaaa atatactttt aaaacttatt caagctgctg
    18001 attttgatat agaaaaagct gaaagaggta taatatatat agatgagata gataagattg
    18061 caagaaaatc tgaaaaccca tctataacta gagatgttag tggtgaaggt gttcaacaag
    18121 cacttctaaa aatattagaa ggtactgttg caaatgttcc accacaagga ggaagaaaac
    18181 atcctcatca agaattttta aagatagata ctactaatgt attatttata ttaggtggag
    18241 cttttgatgg attagagaag ataattcaaa aacgtggtgg agataaaaca ctaggttttg
    18301 gagctaaaat agaaagtaaa aaagaattag atttagggaa gctgtatgaa aaagtacttc
    18361 cagaggattt attaaaatat ggtataatac cagagtttat aggaagaata ccagttttag
    18421 ctactttaga gttgttggat gaagatgcat taatgcagat attacaggaa cctaaaaatg
    18481 ctctagtaaa acagtataaa aagttattgg agttagatga tgttgagcta gaatttgaag
    18541 aaggtgctct tcgtgcaatc gctaaaaagg ccatagaaag aaatacagga gctagaggac
    18601 ttagaagtat agttgaaagc gtaatgatgg aaactatgtt tgaagtacca tctagagata
    18661 atataaagaa ggttatagta acagagaaat ctgtgaacga agactcagta aatcctatca
    18721 tagtacttaa ggatcaagag gaaagtgcat aagatgatga aaaggagctt taaaagctcc
    18781 tttttttatg ttaatatgct aaatataaaa taggtatcat tattataaaa atgtgagtaa
    18841 tatacctaga ataccaaaga cgaatacaat tgcaatggaa ccaatttttc ctttatataa
    18901 aagataaaat gatgctataa atgcaattat tccatataaa tcccaattaa cttctttggc
    18961 agatgaaaaa gctgctgata atatgaatcc tagagtaatt ggtctaagaa ctgtcaataa
    19021 gttttctacc tttttattat ttttaaattt agaaaataac tttgatataa tagttagacc
    19081 tattaatgca aaaattgtaa caccaatagt tgcagctaga gaaccaaaga agccagcagt
    19141 cttaaatcct acaaatgtag cactatttat tgctacagga cctggtgtag attgtgatat
    19201 agcaagtaaa tccaaaaact cttgattagt tataaagtga tatttagata taaattcttg
    19261 ctggataaat ggtagcatgg cataaccgcc accaaaacta aatgccccta ttttaaggaa
    19321 tattaagaag agtagtatta tttctgacat agactatata cacctcctcc aattattaaa
    19381 taaatagggc ttatattgaa tacaccaaca gataaaactg ctataattaa tagtagtatg
    19441 ttagttttag actttggtag tttcttgaac atgctcgtaa aagaatacaa tataagagct
    19501 attacaacag gcgcaactcc tttaaaaaat ccatctaaaa ttttagatga tgaattttta
    19561 aagtatagat acgataagac taagactata caaaatgatg gtaaagcaga gcctaaggta
    19621 catataagaa ctcctggtat accataaagt ttatatccta ataatacaga tatattaact
    19681 gccagtactc caggataact ttgagctatg gataaataat caataaattc ttcctgctct
    19741 aaaagttttt gtttgtttac aagttcatct tgtataagag gaaccatagc gtatccacca
    19801 ccaaatgtaa acgcaccaat ttttaaaaag gttaaaaaca atttcaacat aatagaaacc
    19861 tccaaaagat aaataacatc taagtatact aatattaata ttatatcata ataaaaagat
    19921 aatatagtat gatagtattc taagaattta tcattaatgt tgaaaattaa cttgttcaaa
    19981 tatataatat taaaagcaaa gcaattagac tagaaaagga gtaatgttga tggaacaaaa
    20041 ttatactaaa atagatcatg aattaccatt aattcctcta agaggattag ctatatttcc
    20101 atatatgata ttaaattttg atataggtag agagatatct cttaaagctt tagaccaagc
    20161 tatgatggat gaagaactta tatttttaac ttctcagaaa gaagcagagg tagatgagcc
    20221 aggagaagag gatttttatc atgtgggaac aatctgtaaa gttaagcaaa tgataaaatt
    20281 accaggagat acagttcgtg ttttggttga aggtgtatca aggggtagag taaagaaaat
    20341 agaacaagaa gatgggtatt tcagagcagt aatagaagaa atagtatttg atagtgatag
    20401 tttggatagt gaaacagaag ttgaaattga agcatttgta agaaatgtat ttgatgcctt
    20461 tgaagagtat ataaatattg gaaatagggt atcaccagaa atattaatat ctttagctga
    20521 tatagaagat gtagatagat ttatagatac gatagctgca aacatatatc taaaatcaag
    20581 ccaaaaacaa gaaatattag aagaatttga tattagaaaa agacttgaac ttatttattc
    20641 tatattatta gaggaaatag atatattaaa aatagaaaag aaaataactt taagagtaaa
    20701 aaaacagatg aataaggttc aaaaagaata ttatcttaga gaacaattaa aagctataca
    20761 gaaagaatta ggtgaagaag aagatataaa ctctgaagca gatgagtata gagaaaagct
    20821 taaaaaaata aaagcaccta agacaactaa agagaaaata gaaaaagaga tagataaatt
    20881 ttcaaaaata tcttctatgt ctccagatgt atctgtaagt agaaactatt tagatactat
    20941 attctcattg ccatggaata aagagacaaa agataaatta gatataacta aggcaaaaga
    21001 tatattagat gaagaccatt atggattaga aaaagttaag gagagaatac tagagtatct
    21061 agctataaga actcttgcta agtcacttaa agggcctata atatgtctag ttggtcctcc
    21121 aggaacagga aagacatcta tagtaaaatc aatcgccaga gcattaaata gaaaatttgt
    21181 aagaatatct ttgggtggag ttagagatga agcagaaata agaggtcata gaagaactta
    21241 tgtaggttca attccaggaa gaataattaa tggggtaaaa gaagcccaaa caaaaaatcc
    21301 tgtattctta tttgatgaga tagataagat ggcagcagat tataaaggtg acccagcttc
    21361 agcaatgcta gaagtattag acccagagca aaataaggat tttgtggacc attacttaga
    21421 aatacctttt gatttgtcaa aaatactatt tgttacaaca gcaaatagtc taagtaatat
    21481 accaagacca ttattagata gaatggaagt aatagaagta tctggatata tagaagaaga
    21541 aaaattaaat atagcaaaaa aatacctact tccaaaacaa attaaagaac atgctttaaa
    21601 agaaaacttt ataaaaatag atgatgaaac tttaagaagt ataatcaacc attatacaag
    21661 agaagctggt gtaagaacac tagaaagaac tattggtaag atttgtagaa aggttgccaa
    21721 aaaatacgtt gaagacccaa ctcttgaaga ggttgttata aataagtctg acctagagac
    21781 atatcttgga aaagacatgt ttaagtatca acttgcagaa gttaaccctc aaataggatt
    21841 agtaaatggt cttgcatgga cagaagttgg tggagttaca cttgaagtag aagtaaacgt
    21901 acttaaaggt aaaggagaaa ttgtattaac tggaaaatta ggagatgtta tgaaggaatc
    21961 tgcgaagaca ggtatctctt atataaggtc aatagtggac aagtttgata tagaccctga
    22021 attttataag acgaatgata ttcatataca cattccagaa ggtgctgttc caaaagatgg
    22081 accatcagct ggtataacta tggctcttgc agtaatctca gcacttacta aaagacctgt
    22141 gcctggaaat atagcaatga caggagagat aactttaaga ggtagagtct tagcagtagg
    22201 aggagttaaa gaaaagttat tagcagctca tagagctggt ataacaaaag tattaattcc
    22261 taaagaatgt gaagctgatt tagatgaaat accagaaaat gtaaaagaaa aaatggagtt
    22321 tgtgcttgta gaacatatgg atgaagtatt agaacaagct ttattaaaga gtggtgaaaa
    22381 taatgaaaat tagaagttca gagataacta tgagtgctgt gaataaatca cagtatccag
    22441 cagaaggaat cccagagata gcattggctg gtaggtcaaa tgttggaaaa tcatcaataa
    22501 taaatacatt gcttaacaga agaaacttcg ctagaaccag tcaaacacca ggaaaaacta
    22561 gaactataaa tttttatcta ataaataatg aattttattt tgtagacctt ccaggctatg
    22621 gatatgcaaa gatagctaag tctgaaaaag aaaagtgggg aggtataatg gaacgatatc
    22681 tagaaagtag acaagagtta tgttctatat tcctacttgt agatattaga catgagccta
    22741 cagcagatga taagcttatg tatgaatgga taaagcactt tggatataac tgtgtagtta
    22801 ttgcaactaa ggcagataaa atatctagag gacaatatca aaaacacata agtataataa
    22861 gaaaaaaatt gcaaatggaa agtagtgaaa aagtaattcc tgtttcttca ctaaaaaaga
    22921 ctggtgttga agaattatgg gaagagattg taaatcaata caatcaacat ggatatgaaa
    22981 taacagtaga ttaaatttaa aatgttaaat attgacatga gatttatttg aataataaat
    23041 gtaataatag ttagttttaa ttatattaat attaaagcaa aatataggat gccttaaaat
    23101 agttttaatg aacttttttt ggcatcctta ttgtttataa acaataaata tcagtaattt
    23161 tgttaaatac taataaattt tagtaaggta tgggaaaatt agtataaatt agttcaacta
    23221 aaaaattatt gacttatgta gtcagcaata aatagcattt aagaaataga tttatctgtt
    23281 tttctcataa agaaaacagt tagtataagc cctataaaac atactgatgc tgcaatccaa
    23341 taagactgat tgctagctat aacataggct gatgtgtgac ttttaaactt ttgattgtat
    23401 gtaagacttt gtgcaaaaaa actactaaaa aatgctgttc caagagcaaa tgataaatta
    23461 cgaatagttg caagaaaact gcctccgtat ccctgttttt gaggaggaac atctgctaaa
    23521 atactactat tgtttggtga actaaatgct cctagcccaa gtccaaaaat aactaaacat
    23581 acaattaaaa gatacaatgg agaatctgct tttaaaagtg ccattccaac tagtgagatg
    23641 gtcataaatg aaaaagatgc tgttagaatg ttttttgcac cgtgtttatc agaaagtgaa
    23701 cctgcaatag gtgctataag taccataata agtggatgta cagtcattac ataaccagcc
    23761 atcataggag ataaattttt taattgttct aaataaaatg gaagtagaaa agatactgcc
    23821 atttgaggaa aataacaagc tactccaatg atattgccta cagtaaagtt tctattttta
    23881 aatagaggta ggtcaataaa gctttgttca aattttactt ctcttaatgc caacaataag
    23941 aagattataa gtgctgttat acctaaatac aatcttgtat tacccttctc attcattgca
    24001 agtaatataa gcgtagtgca agatgcgaaa agtagaagac ctggaatatc aaagctcttg
    24061 ttttgttcat ccaaaacagg tttaggtaga aatttaatgc caagaaagac tgctataata
    24121 ccaataggta tattgataat aaaaatagaa ggccatccaa aatgttctaa aattatacct
    24181 ccaattacag gaccactcat atttcctata ccaactacag ctccagtgat tcctatagct
    24241 tttccttttt catttgctgg aaatgcattg gaaactatac caagcccagt agccattaaa
    24301 atacttgctc caactgcctg aaagattctt gaagaaatta atgttgataa attgtttgac
    24361 atactacaca gaaaagaacc tattgtaaat ataaaaaatc ctaatgtgta tagacgattt
    24421 gaacctactt tatcgccaag ttttccgaaa aataacatag tagctgttac aacaagcata
    24481 taagctgtta caacccactg aatttgtgac atgttaattt taaagtcagc agcaattgta
    24541 ggatttgcga tatttagtat acttccatca agagtagaca taaatacacc aatacagatg
    24601 ataatcaata ctatccattt ttgtttcatg ataacactcc aatcttaata gattttataa
    24661 gtacgatata attgtaaaat aaacaacaaa aaaagcacct gtatattagg taccttgaat
    24721 gtgtctaatt tcatgaataa atacggtagt tgaaggattt gtatagatta ctcatgtaaa
    24781 attacataaa ggtactcata tactattttc ttctaccatc cagattttac tgtcggcttt
    24841 ggaatatgac caaattatgc cataaggctc gcgggctttt accgccgata gggaatttca
    24901 cccgtttatg aagaaaaaca gatgtatttt gtaatattat tttaacataa aattataatg
    24961 gaaacaattg gatttaagta atatccagct gtattacaat acaaatattt caatatggac
    25021 acttaaattg tatttataga tagaataagg aacagtatga gaatctataa taattagata
    25081 aaattaacct gttattagta ttagatggta agttttaaat ttatcaaaat cagaaagctg
    25141 taataaaatt cttataatat gaatatctta attaagagat attgtaaatt atgaggaggc
    25201 gctaatatgg aaggaaaggt aaagaaggca ttcccgggtg gaaatacatc acaaggctca
    25261 tattcttttt ttgactatat aattcctgaa aatgtaaaaa gagttttttg tttaaagggt
    25321 ggacctggag taggaaaatc ctcacttatg aaaaaaattt ataaagagtt tttgcataga
    25381 ggatatgata ttgatttata tcattgtcct tctgacccaa gttcgttgga tggtttagtt
    25441 ataaagaaat taggcgtggt acttatggat gcaactgccc cacatactat ggacccaaag
    25501 ctacctggag ctttggatga gataattaat ttaggtgatt actggaatac agaagaatta
    25561 gaaaagaata aacaagaaat atcagtttat gataaagata ttagtaattc atttaaaaga
    25621 gcatttaagt ttttaaaatc agcagaacca atctttagag acatagaaga aaaatattct
    25681 gattgtatgg attatggaaa agtaaatttg gtcacagaag aatttataaa aaggttattt
    25741 gatggagtta aaagcacagg aaatctaaaa agagaaaagc atttatttgg ttcagctata
    25801 actcctgtag ggtgtttaga ttatacagaa aacattttaa aagatgtaaa gaaagtctat
    25861 tatttagatg gagaaatagg aactgggaaa actacattat taaacaaagt atataagaaa
    25921 gctattgaaa aaggtttaaa tgtagaagtt tatcattatc cacttattcc agagaaaatt
    25981 gaaactgtac tattaactga tttagatata ggcataacta taagtacgac ttttaaagaa
    26041 gaagatacaa ttgatttaaa tcagttttta aatagagaaa aactacttaa atatgaagaa
    26101 gatattaaat ttgatgaaaa agttttagat gatttaattt gttgggcagt actaaatctt
    26161 aagagagcca agtctaaaca tgatattata gaaagttact atagtcctaa tatgagattt
    26221 gatgaagtag ggaaattaag agataagtta attaaaagaa ttttaaaata tgaggaaaac
    26281 ttataaacgc atatgtttaa aattaacgtt agtaataata taatttttag taaattggtg
    26341 tagctttgag gctatgccat tttacttttt ttgctaaaaa atgatattaa taatctccaa
    26401 agccattatt tgtttttcat aattattatt tcctttccta atttatgttt ttttataata
    26461 tcacaacaat gtttattgaa agtttatata attttaagaa catctaaaaa tgcatacttg
    26521 atattggtaa attagtgata ggagttcaat gtatgatatt atatactaaa gatgaatttt
    26581 aatatataac tttgatataa taagcatatg taaatgttta atatttaatt aaagaaaatt
    26641 atcatataaa aataaatttg taaggagcaa tacagtatga aaaaacaata tgtgtttata
    26701 atactagcaa gtactatttt aggtatactt gttggaacca tatttagaga ttatagcaat
    26761 gaagaaacag tttatacaag caaagacaag tcaataagaa aagaaataaa aatgacaaga
    26821 aaatctataa aagaattaac caaagagaaa gacaagttag ataaagaaat taattcaatg
    26881 aaacaaaatc aaaaaaattc tgaagaaata tcagaaatag aaacacttag agatagtcta
    26941 tcatattcag atgtaaaggg tagtgggata atattaagta tagatgctat agatgaaaaa
    27001 acagggaata tagctaatag tatagactat aataagattc ttgtaaaagt tataaatgaa
    27061 ttaaaagtaa acggaggaaa atacatagaa ataaatggtc aaagaattaa tcaatattca
    27121 gaagttgtac ttgcaggaag ccatataaat ataaatggag ttcctgtagc acaaccatat
    27181 gaaataaagt gcattggaga taaggagaaa ctatctaatt atgtagataa aggtagtaaa
    27241 tatttagata gcatacaaac caattatcca atgaaaataa gcataaaaac tgaagaaaat
    27301 atctctatga aaaaaatgaa tatacctaat aaattaaagt atataaaggg tgattaagat
    27361 tatattagag gaaagcagtt tgccaatgtt aataccagaa aaacttgcaa aaaaatataa
    27421 tgtatttcca gttgagtttc aagatgataa tttagttgta gaaatagaag atgaggatat
    27481 ttatgctcta caggacttga gattagctac aggtaaagaa attatattga agaaggagaa
    27541 aaaagaactt ataagtgaaa agattgaaaa atattataat agctttgaac ttgatgagga
    27601 ttatgcaaaa aaattatttg aaaatctttt agaaaaagct ataaaagaaa acgcaagtga
    27661 tatacatata gaaccattta aagaacattt aatagtgaga atgagggtag atggagaact
    27721 aaaggaagta tcaaagtttt tagttgatgt ctatccatca ttgtctacgg ttataaaatt
    27781 aaaggcatca atggatataa cagaaaaaag gcttcctcag gatggtaggg tggatataaa
    27841 gttaggtgga aatttaattg acattagagt ttcatcaata ccaactgtct atggtgaaaa
    27901 aatagttttg aggatactta atagagatac ttttttaaaa gacaaattag aacttggttt
    27961 ttcagaggaa gctataaaat ctataaatcg tataataaat aaaagagcag gaatattatt
    28021 agtgacaggt cctacaggta gtggaaagac tacaacagta tattccttat taaatgattt
    28081 aaaaggaata aataaaaata ttatgactat agaaaatcct gtagagtata agatggatgg
    28141 cataagtcag attcaagtaa attcaaaagt agggttgagc tttgatgtag gtcttagagc
    28201 gatattaaga caagacccag atataataat ggtcggagaa ataagagatg cagaaacagc
    28261 taagatagca gtaagagcag ctataacagg tcatttagtt ataagtactt tacatactaa
    28321 tgatgcagta tcttccatag ctagattact agaaatgcaa ataccaccat atcttttaaa
    28381 tgcctctctt ataggtgtta tatctcaaaa gttggttaga aaagtctgca atcattgtag
    28441 ccatgagatt atgataaaag ataattttag tggagatgta aatactaagg tagctgtagg
    28501 gtgtgaaaaa tgtaatgata aaggatactt tggaagaaca gcaatttatg aaatcttaga
    28561 aattaatgat gatataaaga catgtataag aaatatggag gattctagta ctattaaaga
    28621 tgtagcacaa aataatggaa tgataacatt tgaagatagt tgtaaaagac taataaatga
    28681 aaaaataacg acattagaag agtgtttagt agtaaatgaa atgatttaac tgaattgtat
    28741 agttaaactt cgtttggtca gttgtcttta tttaatctat tttaattaag atgtttgagt
    28801 ataaattata tttaagtgaa ataaaattaa atgcattaaa ttagaaaata ttaagtttat
    28861 ttaattgtaa ttaatataat ttggaaggta ttatagtaca tgagattatc taaaaatata
    28921 ttttataatt tagaaaagtt ttatttagta ttaaatagaa tcagtgataa agaaattaga
    28981 gtaatttgca aagagatagg catattgctg gaatctggat gtgaaattac aaaaatattt
    29041 gaaatcattg agagtcagtc aagtaaaaaa gtaaagaact tgcttagtat agtatcaaat
    29101 catattcaaa aaggtaactc aatagcagaa tcatttcaaa taacaggtat attttctaaa
    29161 ttctttataa gtatgataaa agcaggagaa actagtggaa acttagatat aataatgagt
    29221 gatttatcta attattatga taaagaatac aaattaaaaa tgaaaataat aactatttca
    29281 atatatccaa taatacttat aatattatct atcttatcaa tgttgtttat attcgtattt
    29341 gtaataccta attttcaggt ggtttttacc aacaatggaa ttgaaccacc tttaataact
    29401 agagttttaa tgggaatatc tacagtggtt acaaataact tagcttatat aatatttagt
    29461 tttatattgt tttcaatagg aagcatttat ttctttataa caaatgataa tataaaaaaa
    29521 ttgattaatt cgttaaaatt caaaatacct tttataaaaa aaattaatca gttagtagct
    29581 acaacaagat tttcaagggc attgtccgtt ttaattagca gtggaataca aattgtggaa
    29641 gctatagata tgtcagctag tgttatagac aacgaattta tttatgaaag gttaaagatt
    29701 tctaataatt atataaaaaa aggaaatagc ataggtaaat ctttgaattt agcaaatatt
    29761 tttccaaaat tatttatttc aatggcaaat acaggagaag aaagtgggaa actagataaa
    29821 agtctaaatg taataaataa attttatgaa aatgaattaa ataacaaaat agagcaaata
    29881 atgaaattta tagaacctat gctaattgta tttatgggat taattattgg tacttttatt
    29941 ttagcaatgg ttgttcctat gtttgatgca attactaatt tttagagaga aaaataaatt
    30001 ttattagatg aaacttttga aaaggagaga atatttaatt gataaatttg ataaataaaa
    30061 aacgaaaagg atttacactt gttgaaatga ttgtagtagt aactatttta ggcgttatat
    30121 ctagtatagc attagttaag tatagtaagg ttcaagaaag tgccaaatta aatgcagact
    30181 atacgaatgc tgctaatata gtaaccgcag ctagcatggc aattaatgat gatgaaaaga
    30241 caatagactc tctaagtgta gagacattga aggaaaaggg atacctaaat actgttccag
    30301 ttcctcagag tacatcaggt aaattcgaac ttgtcataaa tgatagcgga acagatataa
    30361 gcgtaaatat aaattctaag caattttatc caaaataata ttttatattg attgctgtaa
    30421 gttttaaaat tatttttatt taagaaggag atggctataa ttaaaaaaat tctaaactta
    30481 gaaataaatg atgattatat aaggcttgta tttttaagaa aaagtcataa aaaagttttt
    30541 atagataaat caattataaa agagattaac tttgatataa aaaatgatag taattcgatt
    30601 gagaagatta aatatataga agatgtagtt agatttgtaa aagaggagat tggtaaatca
    30661 aaaatattat ataaaaattt atactttaac attcaaattc aggatatagt cattagaaat
    30721 gttgaagttc taaatactaa gaaaaaaaga gacatattaa gcatgattga atttgaaata
    30781 acacaataca ttccaataaa cataaataac tattcaataa aatataaagt tataaattct
    30841 aaaaatgata aattgaatat acaagtaatt cttttcccaa agaatattat tcaattaatt
    30901 aagaacattt cagaaaatct aaatatgaat ccaaagacta taaatataaa ttttgatata
    30961 ttacaaaagc ttataaactt aaatttaata gagaattttt cagaagaagg ggtatttata
    31021 gaatgtaaaa aaagtcagtt aattataaat ataacaaaag ataaaaaaat aagagaaaca
    31081 tacttgctat caaaagaggg aaaatcatat gagttcataa aaagattatt aaggggtttt
    31141 aagtatgttt attactatgg tatagaaaat tgttttattg aagaatactt taaatataca
    31201 gataaatcta ttgaagtaaa tccattaaga ttaaaagata aagtaaaagt agaaaataat
    31261 tttgatgcag atttagaaga caataggata aattatataa atagtatagg aatgattatt
    31321 taagtgaaag tggataaaac aataaatttt cttaatggat ttaaaaaaac taaaccatgt
    31381 aataaaatct ataaaataat aatagtctta attttaacgt gtattttatt taaatcattt
    31441 atgtatgtga aatctataaa tgaacttgaa gattatataa gtcaaaataa gctagtggat
    31501 aaatcaaaga acactttacc aacaacagat aaaactagag agaaattttc taaaataaaa
    31561 cttaatgaaa taaaaaaaat atatgaaaca attggaataa atagtattac taaaatatca
    31621 atagataaaa ataagctaga gatggaaggt gtgtgtagtg atttagatgt tttaaacaat
    31681 ttaaggaaaa taaagtacat aaaatattat tacatagaca gtattgtaag agatggagaa
    31741 tattatagat ttagaataaa aagttagatg ggtgattatt tttgagatta aataatagag
    31801 aaaaaaatct acttaaaata ttgttaatat tagttattat attggcaagt gatatagcag
    31861 taagttattt ggaagaaaac ctaactagtt tgaagaatga aaagcaaaat ataataaata
    31921 aaataaataa tcaagataat tatcaaataa aggataataa gcatatagat gataaatata
    31981 tagaaagtgt tgaattaaat aaaaatgaag aattgaaaaa taattttaat aactcatttt
    32041 tagataaaaa aacaaataaa gatatagtgt tattagtaga aaatgaactg agagatattg
    32101 tgaatgtaga atatattaat caagaaagta gtttagataa tcacaataaa aaacaagtta
    32161 atttagaaat aaaagttaat ggagaacttg caaatgtgtt gaaacttgaa ggtgtaatga
    32221 agaagttaag actaaataat aaacttgaaa agatagaaat taacaaatta aaagtcaata
    32281 attttgatgt tcaaaatatt ccaaatgaat ataaacaaga aaaagacaat attgttgaat
    32341 gtaaaatgac agttagagtc atataacaaa aaagaataga taaagtaaaa aactattatc
    32401 aattaataaa attttataga ggaattagtt ttacgataat tcatttctac aagagggatt
    32461 gttgatgatt ttatcgaaat cctaattaaa gttaatatat tgtaccaaaa tataagtgaa
    32521 tttaatagaa tatttactta tactatagat tgattaataa aaaaatggta taataacaaa
    32581 ttgtacttta atttaataat aagggagtgg aagtcatatt gttagggaga aatgagctat
    32641 gcccttgtgg aagtggcaaa aaatacaaga aatgttgttt aaataaagat attgtttcag
    32701 aaagagcatg cagaaaaact gaactttcac aaaaacaata tacagattta tattcaaagt
    32761 tatatgatta ttcaagacag gataagttta aagaagaatg tgaaaaagct aaagaaatgt
    32821 tttatataat gcaaaatgat agtgtaaatg aaaaatttga aagatttttt aatacttatt
    32881 taatacaaga ccacataatg gaaaataaaa aggttatgac agttggtttt cttgaagaaa
    32941 atggtgcaaa cctaagtcaa tcagaagtat caatattaaa aagtatgttt gaatcatacg
    33001 taagtattta tgaggtaaaa gaagtttctg ctggaaaaat agtattaaag gactgtttaa
    33061 atggaaatga actttgtaca gaagatgtaa aacttctgag aagttttaaa atcggaagtt
    33121 gtatgatagc tagaatagtt gatattgaag gaactaacat attgatagat ataactataa
    33181 gtatatcaaa tgaggttaaa gatataattt taaaggatat tatgaatcta ttcaatcaat
    33241 atcaagatgt atacaaagat atgaagtcgt tcttaattta tcatacacat atactataca
    33301 agtatataca acagctttta gacccaagta tagctgaata tttaaagaga gaaagagata
    33361 aaaacgatac aaaagttaat tcagaaaatg aattagctga agaagaatgt aaagttttag
    33421 atactcttaa agaaaatgta gaaaaagaaa atatagatgc atgtatagag ttttggaatg
    33481 aatataagaa aaatcatgaa aatataaaag gagctgaaaa tggttgggca gctgctgttg
    33541 aatactatgt taaaaaagaa gcaggacaac ctataactca agttcaaata tcaaagaaat
    33601 acgatataag ctctagtaca ctaggaaaga gatacaagga tttaaaaata tcttaattaa
    33661 ttataaaaag ctttaagtca tagataaaga atctatagta taatatatgt ataatgttta
    33721 gaaagaggta ttaataatga gtcttagttt aggaaatgca attgctgtat caacgaactt
    33781 tataaaatct aacccagctg tacctgtgta caacggagaa gaaatatatg cagttattta
    33841 tcatatagaa ataggatata ataatggtgt atcacctgat accaaacaat atataataat
    33901 agactataaa gagtttgatt tatctatgga taaaaaaaat gaactaataa atattgctaa
    33961 agagtactgt aaatctaaag gtattgtaga aaacgaaaac cttgaaataa accttttaga
    34021 caatgaagaa gcagagaact tcttagaaaa ggtgtttaat aatatgaagg ttataagagg
    34081 tcttataact aaaaaaggat aaataaatgg actatatagg aatagaaaat ataacacctt
    34141 atgaaaatac atatgaattt agtgtatatg aatatgatga tgaaatcacc ttaggtagtg
    34201 aaaagttata tgtatgtgaa ttaagggttg tattgattaa agttaattct ctgtatgttg
    34261 aaagattgca taaatcagtt gaagcaatgg tcttagtaaa aaacttgaaa aaagatttag
    34321 ataaaacact tgttgtaaac aaaataaaga attttgtgct agatgagatt tgggtagaaa
    34381 atctagtaaa agagaatata gaagttatat ttgtagaaag ctaggaataa ccctagcttt
    34441 tttatattac aatctaagag aggaaaacaa tatgttaaga gtatctaatg taaaacttgg
    34501 aattgataaa gatatatctt tattaaaaga tgttatactt aagaagctaa aaataaaaga
    34561 aaaaaactta gtcaaatata gtatatataa agaatctata gatgctagaa aaaaaggaaa
    34621 aatggaattt gtttattctg tagatgtaga agttaaagat gaaaataaaa tacttaactc
    34681 aaaaataaaa gatgtaacaa aaataaagga aatcagatac gtaaatgtac ctgtgggtga
    34741 taaaaaatta aagaataatc ctttggtaat tggtagtgga ccagctggct tgtttgcagg
    34801 tctgctttta gctcaaatgg gttatgaacc aatcatactt gaaagaggtc ttgatgtaga
    34861 cagtagaacc aaagatataa gtgatttttg gacaactaga aaattaaaaa ataattcaaa
    34921 cgttcaattt ggagaaggtg gagcaggaac tttttcggat ggtaaactta caactagaat
    34981 aaaagatata cgatgtagaa aagtattaga agagttagtt cagtttggag caccagatga
    35041 aatattatac tcacataagc cacatgttgg aacagatata ttaaaaaatg tagttaaaaa
    35101 tataagaaat gaaataataa gtttaggtgg tcaagttaga tttaattcaa aagtaacaga
    35161 tatagttgta tcaaatgatt ctatacaatc tgtaataata aatgatactg aaaaaataga
    35221 tacagataca attatattag ctgttggaca tagtgcaaga gatacttatg aaatgttgta
    35281 ttcaagaggt ataaaaatag ttcaaaaacc ttttgcaata ggagctagag ttgaacatcc
    35341 acaagattta ataaataaag cacagtacaa ggatttttat aatcatccta ggttaggagc
    35401 tgcagattat agacttatag agcatgtttc aaatggaaga acagcatata ctttttgtat
    35461 gtgtccagga ggtagtgtca tagcatctgc atcaaatgat tttgaaatag ttacaaatgg
    35521 tatgagtgaa catgcaagag ataaaataaa tgcaaatagt gcatttttag taaatgtaac
    35581 gccagaagat tttaagagtg ataatccact agcaggtgtt catttccagc agaagtatga
    35641 aaaattagct tttgaattag gtggaaaaaa ttataatgca ccagttcagc ttgttggaga
    35701 ttttataaac aataagactt ctttgaattt aggaagtgtt gagccatcat atagaccagg
    35761 atatacactt acaaatttaa gtgaatgttt gcctagcttc gtaacagaga ctatgagaga
    35821 agcgtttata tctcttgata aaaaattgaa tgggtttgca atgtatgatg ctgttttgac
    35881 gggagtagaa actaggtcat catcaccaat aagaatagta agagatgaaa atactttaga
    35941 atcagtgagt attaagaatc tatatccatg tggtgaaggt gctggatatg caggtggtat
    36001 agttacagca gcagttgatg gtataaaatg tgcagaaaaa ataatacaaa aatacaccaa
    36061 taatacaaaa atttaacata aacttaatga ttttttaata tttaacatga atttaacatt
    36121 atcactccta taatttgata aaatacaata ggttataaaa tttttagata cagtaatctc
    36181 tttacaagga ggaaataatt tgaacattat aataagtcta atgggtggat taggtctgtt
    36241 cttatatggt atgaacctta tgggagaagg tcttcaaaaa tctgctggga caaagctaaa
    36301 aaagataata aaactactta ctagtaacct atttatggga gttttggtag gtacaggagt
    36361 tacagcagta atacaaagtt caagtgctac tactgtaatg gttgttggtt tcgtaaatgc
    36421 aggaataatg actttaaaac aggcaatagg agttataatg ggtgctaata tagggactac
    36481 tgttacagcc caattggtat ctttcgactt aactggtatg gccccagtag cattaggtgt
    36541 aggtattata ctttatttat ttggaaataa gcctagaata aaaaatatag ccgaaatact
    36601 tataggtttt ggtatattat ttacaggtat ggattttatg aagatggcag tggagccact
    36661 aagagactat caaggattta ctgacttact tgttacattt ggtagatatc cactacttgg
    36721 attattattg ggatttggga ttacagcaat aatacaaagt tcaagtgcat ctatgggtat
    36781 gttggttgca ctggcagcag aaggtctagt gccattatca gcagcactgc caattcttta
    36841 tggccaaaat attggtacat gtgtaacttc actgttatca agtattggtg ctaacaaaaa
    36901 tgcacgaaga gcagctatga tgcatctaat cttcaatgtt ttaggaacgg tgatattctt
    36961 aatattcttg aataaaccag tagtaagcat ggttacaagt tgggacccaa gcaatgttgc
    37021 aagacaaata gccaatacac atacattgtt taatataata tctgtattga tacttttacc
    37081 atttacaaat cttattataa aactagctat aaagcttgtt cctgatagag caggtgatat
    37141 agatgaagat gaaactaaaa ctattaaata tatagatgat agaatgatag aaactccttc
    37201 tatagctctt gcaaatacga taaaagaagc acttcgtatg ggtgagaagg cgaaggaaag
    37261 cctaaatgca tctatggaag ctttagtaga acattcaact gaaaaaatag ataagacata
    37321 taggcgagag agattaatca atgatttaca gaaagctatt ttaaattatt tacttaaact
    37381 ttcaaaagca cctttagatg atgattcaag agaagttgtg gatactttgt ttaatacagt
    37441 caatgatata gaaagaattg gtgaccacgc taaaaatata gctgaattat cgcaagttgc
    37501 aatagattca aatatttctt tctcagaaga aggacaatct gaacttgatg ttatgtataa
    37561 tagagttgtt tcagcatata cttatgcatt agaatcaatg agaactgata atgttgattt
    37621 agcttgtaag gttataaaaa ttgaagaaca agttgatata atggagaaat cttgtagagc
    37681 taatcacatg tatagattga acaataatct atgtagtata gaaaatggag ttatttattt
    37741 agacgtaata agtaatttag agagaatatc tgaccatgct gtcaatatag cacaacaagt
    37801 tatagcaaag agattaggaa atgattaaaa ataattaatt taaaataata taaaatttaa
    37861 aatagaggat aaataaaaga gaaactttaa atgatacgtt tagagtttct ttttttgtaa
    37921 atttatttga ctaaaatttt taaatttata catagttata gcatttagaa gaatcgattt
    37981 cctgataata aatgatgaga tatgtatgat tttgtggcaa acttttaaaa aaaacaccta
    38041 tattttgtgt tataatctct ttaaaacgaa tatgctgaaa agagggattt aaatgggaaa
    38101 aataaatttt gattatagta aagctacaga gtttttttgt caaaacgaaa tagatgtcat
    38161 gcaaccatat gtagatgttg cgcacgacat gttacataat aaaactggtt taggaaatac
    38221 ttttttagga tggatagacc ttcctaaaaa ttatgataaa gaagagtttg acagaataaa
    38281 aaaatctgct gataagataa aatcagactc agatgttctt ttggtaatag gtataggagg
    38341 ctcttattta ggttcgagag ctgctataga tatggtaagt cattctttta ggaatggact
    38401 taaaaaagag caaagaaagg ctccagaagt atattttgta gggcataata taagctcaac
    38461 atatattatg gatttattag atataattga aggaaaggat atttctgtaa atgtaatatc
    38521 taaatcagga actactacag aaccagctct ggcttttaga atatttaaag attacttaga
    38581 gaaaaagtat ggtaaggaag aagcaagaaa gagaatttat gcaacaacag atgcaagtaa
    38641 aggtgcactt agacagttag ctacagaaga aggatatgag acttttgtaa taccagatga
    38701 tgtaggtggt cgtttttctg tattgacagc agttgggtta cttcctattg ctgctgctgg
    38761 acttgatatt gatgctatga tgaaaggcgc aaatgatgct agagaagcat ttcaaaatcc
    38821 tgacttaaaa agtaatgata gttatagata tgctgttgct agaacaattt tacatagaaa
    38881 aggaaaagat gtagagttat tagtgaacta tgaacctcaa ctacattatg taagtgagtg
    38941 gtggaaacag ctttatggag aaagtgaagg aaaagaaaat aaaggtttat tccctgcttc
    39001 tgtagatttc tcaacagatt tacattctat gggtcaatac atacaagatg gaaaaaggct
    39061 tttatttgag acagtactta atgttgaaaa ctgtaagaga aatataacta taagttcaga
    39121 ggaagtagac ttagatggtt taaactattt agcaggtaag actgtagatt ttgtaaatca
    39181 taaagctttt gaaggaactc tattggctca tacagatgga aaagtaccta atttagtaat
    39241 aaatatacct caactagatg agtataattt tggatattta gtttatttct ttgagaaggc
    39301 ttgtggaata agtggatatt tattgggagt taatccattt gaccaaccag gagtagaagc
    39361 ttacaagaaa aatatgtttg ctttactagg caaaccaggt tatgaaaaag aaaaggaaga
    39421 gctagaaaaa agacttaaat aattttaaat agttaaggtt taattaagat taaaataata
    39481 taatgaaatc atcgaaaagg agtttactca aaatagatga tttcattttt tttatgaaaa
    39541 attgggaaaa atatattttg ataatttata ttatattaag atagggagtg gtatgagatg
    39601 tcaagaagaa agaaagggat aagtttagta atattagttg ctataataag ttcaatactt
    39661 agtagttttc taacaataat tttagttaag gacaatttag taagtaaatc tacaggaagt
    39721 tctacgccga tagttgtaaa tgatgatggt aagagtcaaa atatttatca agcagttgca
    39781 gaaaaagcta cgccatctgt tgtaggtatt acaacaacta gtgttgatac tagtaatatg
    39841 tttgcaatac caactgaaac acaaggtgta ggaactggaa tcatagttga ttcaaatgga
    39901 tatatactta ctaattctca tgttatatca gatggtcaag ctacaagtgt aaatgtatta
    39961 ttcaatgatg gttcaacaac ttcaggtaaa gtagtatggt ttgaccaaca acttgattta
    40021 gctatagtta aggttgataa aactggacta acaccagcag aatttgctga tagtgacaaa
    40081 gtaaaagttg gagatatatc aatagcaata ggtaatcctc ttggattaga ttttcaaaaa
    40141 acagttactc aagggataat aagtggatta gatagaacta tacaaacaga aaaaactaat
    40201 atgactggtt tattacaaac tgatgcaagt ataaatgcag gaaatagtgg aggaccattg
    40261 ttaaatcaaa aaggtcaagt aattggtata aacacagcaa aagcttctca agcagaagga
    40321 ttaggatttg caataccaat caatacagct aagagtatag ttgaagaagt aatcaaaaat
    40381 ggaaaatatg aaaaagtaac tttaggcata aaaggtacag atgtatcaaa ttatgaagca
    40441 gcaacaggta caaaattaag tactgataaa ggtgtttatg ttgcagaagt aatatctggt
    40501 tcatcagcag aaaaagctgg agtaaaggta ggggatatta taactaaagt aggagataca
    40561 gatataacag gtatgaatga tttaaataaa aagttgtata ctttctcaaa aggtgctagt
    40621 acaaaaataa cagtaaatag aggcggaaaa gcagtaacaa taaatgtgaa tttctaaata
    40681 tgaccagaaa gatagagact acatggtaaa gtgtagtctc tatttttatt taatttttat
    40741 agtatataca tctaaattaa ttaaaatgat gtattgttta tagtttattg ctatattttc
    40801 ttataaaaat aaatttaata aatatttaaa tttaattta
//
LOCUS       DAMJZE010000018        37930 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJZE010000018 DAMJZE010000000
VERSION     DAMJZE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.018"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(62..949)
                     /locus_tag="PC541_000840"
     CDS             complement(62..949)
                     /locus_tag="PC541_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2838314.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            1068..2267
                     /locus_tag="PC541_000841"
     CDS             1068..2267
                     /locus_tag="PC541_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2838315.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(2461..5016)
                     /locus_tag="PC541_000842"
     CDS             complement(2461..5016)
                     /locus_tag="PC541_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2838316.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5013..5699)
                     /locus_tag="PC541_000843"
     CDS             complement(5013..5699)
                     /locus_tag="PC541_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838317.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(5776..6684)
                     /locus_tag="PC541_000844"
     CDS             complement(5776..6684)
                     /locus_tag="PC541_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838318.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(6686..7357)
                     /locus_tag="PC541_000845"
     CDS             complement(6686..7357)
                     /locus_tag="PC541_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838319.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            7619..8683
                     /locus_tag="PC541_000846"
     CDS             7619..8683
                     /locus_tag="PC541_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2838320.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            8841..9527
                     /locus_tag="PC541_000847"
     CDS             8841..9527
                     /locus_tag="PC541_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838321.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            9517..10548
                     /locus_tag="PC541_000848"
     CDS             9517..10548
                     /locus_tag="PC541_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838322.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            10630..11295
                     /locus_tag="PC541_000849"
     CDS             10630..11295
                     /locus_tag="PC541_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838323.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            11308..13725
                     /locus_tag="PC541_000850"
     CDS             11308..13725
                     /locus_tag="PC541_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838324.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(13906..14697)
                     /locus_tag="PC541_000851"
     CDS             complement(13906..14697)
                     /locus_tag="PC541_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2838325.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(14730..15317)
                     /locus_tag="PC541_000852"
     CDS             complement(14730..15317)
                     /locus_tag="PC541_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2838326.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(15494..16885)
                     /locus_tag="PC541_000853"
     CDS             complement(15494..16885)
                     /locus_tag="PC541_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2838327.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(17069..18373)
                     /locus_tag="PC541_000854"
     CDS             complement(17069..18373)
                     /locus_tag="PC541_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2838328.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(18732..19607)
                     /locus_tag="PC541_000855"
     CDS             complement(18732..19607)
                     /locus_tag="PC541_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2838329.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(19811..20497)
                     /locus_tag="PC541_000856"
     CDS             complement(19811..20497)
                     /locus_tag="PC541_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2838330.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(20546..21079)
                     /locus_tag="PC541_000857"
     CDS             complement(20546..21079)
                     /locus_tag="PC541_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2838331.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(21123..21755)
                     /locus_tag="PC541_000858"
     CDS             complement(21123..21755)
                     /locus_tag="PC541_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2838332.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(21845..22924)
                     /locus_tag="PC541_000859"
     CDS             complement(21845..22924)
                     /locus_tag="PC541_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2838333.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSDVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(23057..24403)
                     /gene="pepV"
                     /locus_tag="PC541_000860"
     CDS             complement(23057..24403)
                     /gene="pepV"
                     /locus_tag="PC541_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2838334.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(24472..25752)
                     /locus_tag="PC541_000861"
     CDS             complement(24472..25752)
                     /locus_tag="PC541_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2838335.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(25879..26139)
                     /gene="hfq"
                     /locus_tag="PC541_000862"
     CDS             complement(25879..26139)
                     /gene="hfq"
                     /locus_tag="PC541_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2838336.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(26253..27188)
                     /gene="miaA"
                     /locus_tag="PC541_000863"
     CDS             complement(26253..27188)
                     /gene="miaA"
                     /locus_tag="PC541_000863"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2838337.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(27226..29193)
                     /gene="mutL"
                     /locus_tag="PC541_000864"
     CDS             complement(27226..29193)
                     /gene="mutL"
                     /locus_tag="PC541_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2838338.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(29204..32047)
                     /gene="mutS"
                     /locus_tag="PC541_000865"
     CDS             complement(29204..32047)
                     /gene="mutS"
                     /locus_tag="PC541_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2838339.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(32207..32941)
                     /locus_tag="PC541_000866"
     CDS             complement(32207..32941)
                     /locus_tag="PC541_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838340.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(32910..33923)
                     /locus_tag="PC541_000867"
     CDS             complement(32910..33923)
                     /locus_tag="PC541_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2838341.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(33945..34715)
                     /locus_tag="PC541_000868"
     CDS             complement(33945..34715)
                     /locus_tag="PC541_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838342.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(34690..34980)
                     /locus_tag="PC541_000869"
     CDS             complement(34690..34980)
                     /locus_tag="PC541_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2838343.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(35111..35210)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      35830..35919
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            35972..36148
                     /locus_tag="PC541_000870"
     CDS             35972..36148
                     /locus_tag="PC541_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838344.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(36263..36619)
                     /locus_tag="PC541_000871"
     CDS             complement(36263..36619)
                     /locus_tag="PC541_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838345.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            37086..37834
                     /locus_tag="PC541_000872"
                     /pseudo
     CDS             37086..37834
                     /locus_tag="PC541_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
BASE COUNT        12870 a         5947 c         4386 g        14727 t
ORIGIN      
        1 tcaaatttac gaatttatct ctatttttat attaaattac ttgtgaaata tgtattctaa
       61 attatatatt attagatatc atgcttaaaa attctttggt tgctaaggat aaagatatat
      121 cttttaaatg gcaataacct atatttctct taggtattgg ctcttttata tctaatttaa
      181 acaatttctc attttccaaa taatctatgc taaattcttc tataacacaa gacactccaa
      241 gatttatata cgcaaattct aaaagtaatt catgagaacc caattctata tctgggttta
      301 gttttatacc ttttgataaa aaatattcat ttacataaag tcttgaattt gctttatttt
      361 ctagcattat taaaggcaat gtgtttaatt cttccaatga tattttccta cctttgtatt
      421 ctatgtaatc atttgcacat acaaatatat catgtatttc aatacactca acaatattta
      481 gtgtatcatc ttctattggc atgtttataa ttgctatatc tatattccca tctttgagta
      541 aagatataag ttctctactc gttctattta ttgtagatac atgtatatgt ggatacttct
      601 tatgaaaaat ttctaaatat tttaacaaga aaaaacgtgc agctgtatct cctactccta
      661 ttttcagaga accgtattct aattttttaa atttatttat tctatcttct cctgatgata
      721 ttaactttaa tgcagtagtg acatgttcac ttagaatttt cccctctgga gttaatttta
      781 ctcctttatt gtttctataa aataataaag tatctaattg ttcttctaat tgctttattg
      841 actggcttac agctggctgt gacataaaca attccttagc agcaagagaa aaactcttat
      901 tatttacaac tgcattaaaa accttataaa gttcaagttt tatattcata tataattact
      961 ccttatgtca aatattagta ttatttattt tacttataat tatatttgaa ttataatata
     1021 catggtacta atatgacaat attaattctt taggaggatt ttagataatg gatagattag
     1081 ttggaactgt ttcaagaggg gttagagcac ctattataag acaaggagat gacttagtta
     1141 aaatagttgt tgattctgtt ttaaatgcgt caaaaagtga aaactttgaa gttagggaca
     1201 aagatgtaat tgccgttaca gaagctgttg tagctagagc acagggaaat tatgctcacg
     1261 ttgacaatat tgcaaaagat gtaaaagata aatttggaga tgatactgtt ggagttatat
     1321 tcccaatttt aagtagaaat cgttttgcta tatgtttaaa aggaattgca aaaggatgta
     1381 gaaaagtcgt attaatgtta agctaccctt ctgatgaagt tggaaatcac ttaataagta
     1441 ttgatgaact tgatgataag ggaataaatc cttggtcaga tgttttaaca gaagaaaaat
     1501 acagagaact atttggatat aacaagcata catttactgg tgtagattat gttgattatt
     1561 ataagaattt aatcaaggat tgtggtgcag aggtagaaat agtatttgca aacaatccaa
     1621 aaactattct taactataca acaagtattt taacttgtga tatacataca agacaaagaa
     1681 caaagagaat tttaagacaa aatggagcta aaaaagtata ttcacttgat gatataatga
     1741 cagcaagtgt agatggtagt ggttacaatg accagtatgg acttttaggt tcaaacaaag
     1801 ctacagaaga aacagtaaaa ttattcccta taaattgtga tgaagttgta aacaaaatcc
     1861 aaggtaatat aaaagaaata acagggaaag atgtagaagt aatggtttat ggagatggag
     1921 cttttaaaga ccctgtaggt aaaatatggg aattagctga cccagtagta tctcctgctt
     1981 atacaaaagg gctagaggga actccaaacg aagtaaaatt aaaatactta gcagataatg
     2041 attttgctga cttatctggt gatgagttaa aagaagcaat ttcaaaatac atagttgaaa
     2101 aagataataa atctgatgat ttaacaggaa acatggtttc tcaaggtact actccaagaa
     2161 gattaacaga ccttataggt tctttagcag atttaacatc tggaagtgga gataagggta
     2221 ctcctattat ttacatacaa ggatatttcg ataactatac aaaatagtat gttttaataa
     2281 gatttaattt ttttggatta tatatattaa catgaaaatt tattaaaacc tcttaataga
     2341 tatgactttt caagagttat atctattaag aggtttattt aatgttcaca gataagatat
     2401 atttaaataa tttaaacaag attgtcagaa agttatataa tcttaaaaat ttatataact
     2461 ctaattctta tttatttctg aaataatatc actcttcaaa atttgtctta ctggaataat
     2521 tacagcaact atactaatag tgatatttaa aataatcata cctatgtata gaggtgtatt
     2581 tattttaaga attggattta tatataaata tacacgtttt accataaaat aaatcataaa
     2641 ttgtccaata acacttccaa ttaccattat aatacttgca tagaatccat ataaaaaacc
     2701 ttctcttatc atcataagta aaaacttatt atccgtaata cccatagctc ttaaaattcc
     2761 aaactcatgt cttcttgaaa aaattaagta gcttactgta ttgattatat ggaacaaact
     2821 tattgcaaga agtacaaata caataccata tataaataag agtttttgat ttaagtaggt
     2881 attatttttt tcaatagcaa tggtataatc tgttacgata catcttttta catcacttac
     2941 aacacttttt atgttctcag acaattcctc actgttacta tcttctgtct tttttatact
     3001 tactgaatta taattttcga tatcaaagtt atttttcatt tgcccattcg tcataactat
     3061 atccatacca taatcaccaa tgaaatatat gttatttgcc atcacacgtt ttacagtagc
     3121 agcaactaca aattcttttg ttgtataatc attcttattt tcttctttaa atttaacaat
     3181 ttcatctgtt ggtttcattg tctttggcac tttaactttt attgtatctc ctggatttat
     3241 atcaactgca tcatacatcc cagttccatc cataggtagt ctaactataa ccttattatt
     3301 tttaaccata tcatgggtat taatctcccc atctataata taagaattta atccttctaa
     3361 cataccattg ttataaccat aaatatttgt ttttaaaaga tagccacctt cattttgctt
     3421 tgtacaaata ccatcaaaaa aagtctttat cctgtaatct ttttcaagag gtttaaagaa
     3481 atttttccaa aatagttgtt cttctttaat ttgcatagct ccaaataaat atcttactgg
     3541 atagactttt gatattccct caacattgtt taatcttgaa attttatctt ttggtatacc
     3601 tacatcaaaa tctgttgttt gcatagttat ttggtagtca gaatttaaac catcatcagc
     3661 tttcattgta agttcattat tatttttagt aagctgtata gagtaactag aacataaaaa
     3721 tataattcca ccaagtgata atgaaaacaa aattccaata aacattccct ttttttgagt
     3781 catgtattta tgagataata cttttgtcat gttagagtgt tttacacttt ctaatattct
     3841 ctttttctta ttagtaatct taaagttttt catagattct atactagagt atttattcaa
     3901 ctgatgtaca acacgtacag caattaaaat taacaacaat aagataaata aaaagcctaa
     3961 ataaatagat ttttcactta taaaaaatgc tcctggaaca atatcactac tcaaaaatat
     4021 atgtggaaat tgttcatata ttgtagacgc cacttcaata cctaagaagc aacctatagg
     4081 aaatccaaca ataaaaagta aaaataactc taaaaatagt agaaataaaa gttgtttatt
     4141 attagcgcct aatacttcta aaaccccata ttgtgatatt ctctgaagta ttgaaacatg
     4201 gaaaatacta tatactgctg ctgcaccaaa cactccaaca agtaatataa ttgcatttgt
     4261 atttagttct aaatttttta agaactcaaa tatattatat ttttctatat tttttggtgg
     4321 ttttactcca aattcaactg aaagaggcca gtttatataa gttttttctt ttgatatgtt
     4381 gaattttttc ataaatttat aattttgctt tttaatattt ttattttcat catattttac
     4441 ataaactata taagaatcct tttctttagg ctgaaaataa tcgtttacaa atccctccag
     4501 agcagtttct gggtttgaaa gacttgataa aataccacat aacttataga ttttaccatc
     4561 taaattgatt tctgttccaa tttctttgtt tacatttaaa ttgtttagag aatatgtgtc
     4621 aagtactatc tctccatctt tactaggaag agttcctttt aataattttc tacctgtcat
     4681 gtctaaatat gaagaatcag catttaaaaa ttctataata aaaggttcat ctatctgatt
     4741 tttaggtttt gtaacgccaa gtctatctac agtgtattcc tttgattttt tatttttttt
     4801 aattttatta agttgttcct tatctacttt atagaaataa tgataatctc cactatcttc
     4861 ccttatcttt tcaattctac ttttgtctgc actatgaact aaagaaccaa ttcccgtcaa
     4921 taatgctact gatagtacta tacttaaaac tatagatata gattgttttt tataatgctt
     4981 tatatattga attgaaaatt ttactagaga catcatagta cttcaccacc agtattcacc
     5041 acaactccat cttcaatatg gataattgag tcgcatactt gtgctatatt ttcattgtgt
     5101 gttaccataa gtatcgtttg atggaatttt gcactacttt cttttaataa acataccact
     5161 tccattgtag ttttactatc gagatttcct gttggttcat ctgctaatat aagcgcaggt
     5221 ttatttgcca aagctctagc aattgctacc ctctgttgtt gaccaccaga tagttcgctt
     5281 ggaaatttta aacgcttatc ccaaattccc aatgtcttca tcagcatttc aatatattca
     5341 tggtctatat ggtttccttt atctaatgta acaggaagag ctatattatc ataagtgttt
     5401 aatacgggca ttaaactgta gttttgaaat ataaatccta tatttcttct tctaaacact
     5461 gtcaaatctt tttttcctat tttagatatg ttctttcctt taattattac atcacctgag
     5521 gttggattat ctaatccacc tataatattt aacaatgtgc tttttccaga accagaagtt
     5581 ccaactatag cagtaaatgt atctcgttca atttctatac ttactccatt taaggctttt
     5641 actttagtct caccttttcc atagtgtttt actaaattat tcgtcactaa tatactcatt
     5701 taatttctcc tttcaataca gatatatgtt ctataaaata ttgtaagtga cgataattac
     5761 actcatccta caaaattagt ttaaactctt acaattctgt aagaagatac taaaacaaca
     5821 tccacttcct agtttagatt ctactataat gtttccattt tctttttcta aaattaatct
     5881 tgtaagatat agaccaatcc ctgtgccatt ttggttttca acatttttac ttctgtaaaa
     5941 tcttttaaaa atatcattta gttctctact atcaatacct attccctcat ctttaatagt
     6001 aatcttcgta tatagttcca tttttattac agaaatagta atcgtgctat ctgatggtga
     6061 gtattttatt gcattttcta aaacatttac aattgcttct atggtccact ttttattatg
     6121 aactaatttt atgtctgaaa atggttcgag atttatcctt atatttttag cttctgcttt
     6181 attaaagata gtactgatac tttgaataag agtatcttta attagtagtt cttctacttc
     6241 aaattctata actccatcct ctaatttagt cattttaaat aaggattgta acagccagtc
     6301 aatcttaaaa gtttcatttt tcatcttttg taaaaactcc ttcttctgta atgtggaaat
     6361 atttatattc tccagaagtt cacaataaat ggttatattt gaaagtggag tttttaattg
     6421 atgtgacata tttgatatta aacctttaat cgcttctttt tctgactttg actgttctac
     6481 ctctatttct atcatatcct taattttttt tacttggtga gaaatttttg acgctcttgt
     6541 atcctttgta tctggataag taacttcttt accttccaat atttgattta acatcaaatt
     6601 taattcatca tatctttttc gatttatttt agtagatact ataaatataa caactgtaca
     6661 tattataaag gctaatataa acatattatt ctacctcatt ccataaataa cccattccat
     6721 aagctgtttt tatatatttt ggattagatg ggtcatcctc tacttttact ctaagtcttc
     6781 ttatatttac agatacaata ttatcatcaa cataattttc ttcgctgtcc caaatatggt
     6841 gtaaaatttg ttcctttaat aatatttgac ctttattttc aattagataa cttagaagct
     6901 tatattctac agagctaagt tccaaacatt ctttattttt atatactttt agtaactttt
     6961 ggtccaattt tattccattg gaatatatta ctttattatt cgattttttt cttattgcta
     7021 ctttgactct tgcctttaat acagataaag aaaatggttt tgtaatgtaa tcatcaagcc
     7081 ctatttctaa tccttttact tcatcaattt ctgagtctct tgcagtaagc attataattg
     7141 gtatatctga cttttccctt attatttgac atacatcaaa tccatctcca tctggaagat
     7201 tgcagtctaa gattataaaa tctatatttt tactattaaa tatatttaat ccttcttgtt
     7261 ttgtataagc cttaaataca ttaaatccat cattctcaaa tgcataaaaa agaccttcat
     7321 ttaagtttat atcatcttca ataattaata agttcaattt tactctcctt tagatttttt
     7381 agataaataa ttattttctt gcattatcat actaaaaata ttttaatttt atttatttca
     7441 ctaaaaagat tgtatttaaa tttgttttca aaataaaaac attataatca agcatatagt
     7501 tatatatttc ccaatttaat tacgcttaaa ttgaaaatat aactttattc ttatataatt
     7561 aaattataca acaataaagt tatatttttt aaatatattt ttactagggg gaatagaatt
     7621 gaaaagggtt gatgagagaa atactatgtt tgcaagagca aattacaaaa aagatagtac
     7681 tgcttataat gactattata agaaaaatcc tgacaaaaaa gaaattgatg atagtataag
     7741 aaatagacca aatttatgta gtgaaggaac aatgacttac aatgagttaa actctccaat
     7801 ggcaagtagt gcattcgatt ttttatctga tatcaaatct ttatgcgaag gaaaagtttc
     7861 tgatactaaa gtagatgtag atgcaaagtc aatgacaaaa aaaattaagg gtcttgcaaa
     7921 gcagtatgga gcttctgtag ttggtataac taaattaaaa gactatcata tttatacaaa
     7981 taggggtaga catgaagaaa attacggtga agaaattaat ctaactcata agtatgcaat
     8041 tgtatttgga tgtgagatgg ataaagaaat gataaataga gctccaatga tatgtgaagt
     8101 tatagaaaca tcaaagtgct atgtggatgc atctatcgtc ggcatgattt taagttacta
     8161 tataagaaat ttaggatatg atgctagaaa tcatatggat gctaattatt tagtaatgcc
     8221 tgtttttata gctcgtgatg ctggtttagg agatatagga agaaatgcta tacttacaaa
     8281 taaagattat ggctctagac ttagattagg tgttgttact acagatattc ctcttttaga
     8341 agatgaatat gttgattttg gactagaaga tttttgtaag gtctgtaaaa aatgctcttt
     8401 taactgccct tctcattcgc tttctaatga tattaaaatt ggtgatgatg gaaaatataa
     8461 ttgggtaatt gaacatgaga catgctatat taaatggaga tatttaggta cagattgtgg
     8521 tatgtgtata tctgcttgcc catttagcca aaatctcgaa actataaaga atactacttc
     8581 atttaagggt aataatgagc ttattcaaaa agctttagat gagtatactt caaaatttgg
     8641 taaaagaata tttatacctg gaaatccatc ttggttgaaa taattttatt taataaagaa
     8701 tctgtactag aatttatcat aggacaggtt ctttattaaa tatataaatt tttaaatact
     8761 ttttctcgtt tataacaatt cagcaatgtt tttagtctat aattgacatt ataaaatatt
     8821 aaactaaaag gagtaactgt atggaaaaaa tattagtagt agaagatgat tctatattaa
     8881 ataaaactct atcatataac ctaattgaag atggatatat aattacttca aaatttacag
     8941 ctaaatctgc actaaagtct atttttgaat gtgaatttga tttaataatt ttggatatta
     9001 atttaccaga taaaagtggg tttgaattat gtaatgaaat aaaggaaaat tacaatatac
     9061 caataatttt tttaactgct aatgatatgg aatgtgatat gattaaaggc tatgaacttg
     9121 gagctttgga ttatataaca aaaccattta atatcaatat atttaaacaa aaaattaaag
     9181 cttttttaaa tcatcttaca ataaaaacaa aacaagatta ctatagggat ggatatcttg
     9241 aaattaactt ttctgagtta tctgccaata taaatggaaa tcaaattata tttacaccat
     9301 tagaatatcg tactttaaaa ctgcttactg aaaatcctaa aagtatttta acaaggaaag
     9361 tacttttaga aaaactctgg gatatagatg ctaactttgt agatgaacat actttaacat
     9421 ctgttataag taggattaga agcaaaattg aaaaagacaa tcttaagtat atcaaaacgg
     9481 tatatggtat gggatatatg tggttaggtg aaagaaatga attttaaaaa attttctttt
     9541 agttctattt atttaataat tgctgttggt ataattttta cagctatttt atttagtatc
     9601 tcaatttatc taataactga tgatttaaaa ctaattgctc tattattaat atatacaata
     9661 gtactttgtg gatttatagc attaataata tttttacttc ataaaaaaat agttatattt
     9721 tgttctagaa tatgtcacac tttagatagt atgatgaata gtgaagataa tattgaaatt
     9781 gaattagaag aagaaactat tttatcacgt atcaatcaca gattaattag aatgtatgaa
     9841 gttattcaag aaaatcgcaa tagtattgct atagagaaag ctgatttaca agaactagtt
     9901 tctgatatat ctcatcaaat taaaactcct attgcaaatt taaaaatgat taatactact
     9961 ctactaaatc aaacatttaa aacagaaatt caaaaggatt ttttaatgga tatggaaaac
    10021 caattagata aattagattt tttaatgcaa tctatggtaa aaacctccag actagagaca
    10081 ggtataatca cactatctaa aaagaaaaat tcaatttatg aaacaattgc tacagcactt
    10141 agtggaattt tgtttgatgc tgaaaaaaag aatattgaag ttacagttga ttgtgaccca
    10201 aatttatata taattcatga caagaaatgg acttctgaag ctatttttaa tatattagat
    10261 aatgctgtaa aatacacaag ttctaatggt aagatacgtg tagctacaga atgttgggtt
    10321 atgtatacaa aaatagatat tattgataat ggaaaaggaa tatcagaaag tcgccaagca
    10381 gaaatcttta aaagatttta tcgtgaagaa gatgtccatg acatagaagg tattggaata
    10441 ggtctctatc tttcaagaaa aattatatct cttcaaggtg gctatattaa agttacatca
    10501 gatattggaa aagggtcgac tttttctatt ttcttaccta ataaataaat ttttaaatat
    10561 ttcatttgta atgattctgc aacattttgg ttttatactg tatttacaag atgttaagga
    10621 ggtattatta tgagtatttt acaagtaatt aatttaaaga aatattatgg acaaagttca
    10681 aacattacaa aagctcttga tggtgtaact ctatctattg aaaaaggcga atttatttct
    10741 attgtaggta catctggtag tggcaaatcc acattattaa atatgatggg aggattagat
    10801 aatcctacat ctggaaaggt tattataaag ggtaaggaat tatctactat gtctgatgaa
    10861 cagcttacaa tctttagacg cagaaatata ggttttatat ttcaaaatta taatcttgtt
    10921 cctttactta atgtatatga aaatattatc ttaccaatag gattggatgg agagaccata
    10981 gataaggatt ttataaatga aattataaat atgctttctc ttgaagaaaa attatatgat
    11041 ataccaagta atctttctgg tggtcaacag caacgtgttg ctatagctag agcacttgta
    11101 acaaaaccag ctattatact agctgatgaa ccaacaggta accttgatag tagaactagt
    11161 tcagatgtac tgagtttact taaagtgaca agtaaaaaat tcaaccaaac aattgtaatg
    11221 attacacaca ataatgaaat tgcacaacta actgatagaa ttattcgtat tgaagatgga
    11281 aaaattgtta actaggaggt gtagtatatg tttagaaata ataatcgcac atttataaga
    11341 aaaatagctt taaatgattt aaaaattaat aaacttaaaa catatctttc aggtatcatt
    11401 ataatgattt ctacatgttt attattaaca gtgaccttag tttcatataa tgcctctgta
    11461 gatttagtta atgcatctcc atatcatgct atatataaat ctgtagatga aaaagcaaaa
    11521 aatatactat ataaagataa aaactttagt ggcgtaggaa cttacaaatt aattggaagt
    11581 aataaaaaga cagattgtat tatgtcgatt gtttatgcag atgatacagc aattaaactt
    11641 atgaattttc aaccattgaa aggcaaacta cctacaaata aaaatgaaat tgctatttca
    11701 gaaaaatatt tacaggaatt tggtttagat aaagatattg gagatagcat taaattaaat
    11761 tattatgatg atattacgaa taaagaagtt caatgtgatt ttattattgt tggatttcta
    11821 gaaaattatt atcaagataa tgccaagcaa tactatacag tcgtttcaaa tgattatttt
    11881 aagaatatag cttctacacc tttacaaaat aaaagtaata cttttgacga gtcaagacct
    11941 gatactgtag atgtattagt taaactatca gaagaaaata cagagaaaga ttcagcttca
    12001 attaagacac agttaaataa aattggttta tctttaggta ttaagaaata taatatatac
    12061 ttaaatgata actatattga atcaaattta attgatggtg agcagattat tacagtttta
    12121 gtagttggaa tagttgttct tttttctagc gtttttgtta tatatagtat tttttatatt
    12181 tcagttgtta attcagttca gatgtatgct aagttgaaat cgttaggtat gacttctttt
    12241 cagttaaaaa aaattatttc tctacaagga aatattttgt caattatttt tattccatta
    12301 ggggttatag cttcttgtat aattgcttac ataattcaac cattagcatg gcaaatgata
    12361 gctgatttat ttataatttt aattttatca ctggtaatgt ttttaactgt tagaatttct
    12421 ttatttaaac cagctagaat tatttctaaa atatctgcta ttgaagccat gcaatataca
    12481 gaaactaaat ttagaaaaaa agcaaaaaat tttagttata taaatataaa aaatctagca
    12541 ttaaagaata tcgagtcaaa tagaaagaaa aatttgattg cattaatatc gctaagcatt
    12601 agtggagttt tatttatttc aatcgctaat cttgctaatt caatagattt caaaaaacaa
    12661 ctatctcaac aatttgtcta taacgaagat tatataatta gcattaagag tgataactta
    12721 tatgagcata ttacagaaat ccaacaaaat aatccactaa cagattctct taagaatgaa
    12781 attaaatcta ttcatggagt aaaaaaatta attgaatcca aatcaattaa aagtaatatt
    12841 atagaacctt acatcaaaga tagagacgaa aaacaattta ttactctaat taaaggtatt
    12901 acgcctgaac tttcaaaatc acttgaagaa tatattgtca gtggaaaaat agactataca
    12961 catttagatt cagatagttt aattataaat aaatatagaa caaaatatta tggattaaat
    13021 ttaaaagttg gagatacagt tacatttaat atctttagtg gaaatgatat tattaaaaag
    13081 aaaatgaaaa ttataggaat cattgacaat tccaatacag gaggtatgtt ttttgcttca
    13141 gacaaaactt tagaatctct aacaccatat aatacaaatt tagatttttc tattttaata
    13201 gataaaaagc attctaaaga agttgaagaa aagttgttaa gtataattca gaaaaattca
    13261 aatttaatgc tttattcata tgaagatgat tatagaatga ttactagagc atttcaatat
    13321 ataattatag cttcatacat ttttgttggt ttaatttctt gctttggtat actaaatatg
    13381 accaataccc taataaacag cgttttaatt agaaaaaaag agtttgcatt acttcaagcc
    13441 gttggaatga caagaaagca actgcgaaat atgctttata gagaaggtct aaacataagt
    13501 atcaaagcta tatgtacttc aagtatacta ggttattttg gttcaaatct actttgcact
    13561 tttataaaag atgtaattag attagacttt attaatttta aattttctat atttactatt
    13621 ttaatttttt cattcgtatt aattggtata caagttttag tgactgaatt acttgtaaga
    13681 aatatagaaa aaaaatcact tacagaacgc ttacgttctc agtaagaatg tatataaaaa
    13741 atagccattt tttatacaag ttttaacata attttttaaa agctaaaagg atagaaaatt
    13801 ttgactttac ataattttct atccctatac ttgtttttat tttatacaga tacacttact
    13861 tatatctccg agcaacatca ccagagacat aaacaagcat attccttatt ttctaaatat
    13921 gtcaattgca tgacttgtaa cccctactaa atcttcacgt tcacaagtag caaggtgata
    13981 ttttaaatat aatgcaaatg catcatcatc catagagtct actgcttcac gcatatacaa
    14041 agccaaacca tctgatgcga cataatgcag tctagttaca ttaaatgcag acatcaaatc
    14101 atcaatgttt tctttacgta caagctcaaa caaatccttt ggttcagact tagcagcaaa
    14161 tgtctgtgga tcaatcaaac ctttttcaat atattcagat acattaatat ttcctctatg
    14221 gaacccctcg tcaataagac agccatcaga tataacatat gcagcaaaaa ccacaccacc
    14281 aggttttgtt acacgaattg cctcatgtaa agcttgttgt ttatcttctt tgttataaag
    14341 atgatatagt ggtcctaaca gtaacgtaat atcatattta ttatctggga aaacagataa
    14401 atccatagca ttaccctgag taatgcttat attttcattt gattgagtat ttttatggaa
    14461 aacctctata ttatgttcga ctaattccac tgcgtcaaca tcatatccct gacgtgccag
    14521 tgtgtgagaa taacgaccag ttgcagcgcc aatttcaagc acacaatcac ctgattttat
    14581 gtacttatca atgtaatgca tagtagtgag aaattccacc attccatgtt ttaatgcaag
    14641 tcgactatct tcatcataag tattataaaa gtcaattaag tattgttttg tttccataat
    14701 ttcacctctt ctatattagt taataaacat caaatagaca aattccaatc tgatttttct
    14761 tgtcttataa aagctgttaa tatatttttc aacatttttt cagcactata tctatctaat
    14821 gttaattttt ttgttgctgt taagacagct atgccatgag tataaagaaa taaatctaaa
    14881 aatattactt ttgcacattc caaatctatt ccaatgcttt ccgaaaaaag tcttgccttt
    14941 ttttcattgt caatttcttt ataaaaatca ttcgcctctt tcatcatcaa atctaaatca
    15001 tttataaata acaacttaaa taaatacgtt tcttcttgag caaattcaat gtatgaaagc
    15061 ggaagcagta aataagggct tacactttta gaattacgat aattaacaac atactgatta
    15121 taatattcat atgcaaaagc aagaaattca ttttttaatt cattcatgtt ttcataacaa
    15181 gtaaaaattg gtcgagtgga acactgtaat ttacttgcaa tactcctcgc atttacagct
    15241 tcaaatccca cttctcttgt aatatccaag actgtattca aaatcatttc ttttgttatc
    15301 tttactttag gtggcatata tgttcacctc tccattaaat ttttataaaa cacatgtgtt
    15361 atataactat agtaacatat caagaaatta tgtcaagaaa aagctttgca tttttttgca
    15421 attcctaaaa cttataaata aaacatgagt tgcaattaat ctattaatag ataacagcaa
    15481 ctcagatttt cctttaaata ctcttctttt tgtctaatat ttcatataca caatcttcta
    15541 ttgaattaat atctttttct tttctttttt tagaatcaat taaaatgaaa tacagtataa
    15601 aacctacagc aagtccccat gttccgcctt tagcagcaat tacacctccc ataagtccaa
    15661 tcaatccctt ctcaacacct gtattacata gctccattga tagctttgta cataaatatc
    15721 cttgaactaa aagtgttact gacaatgcta atggtataag aggtgtagca atctgcgcaa
    15781 ttggataaaa acagataaaa attgctgaaa cccatactaa tgtacccatg ccactaaaaa
    15841 cagactgcat ggcttccttt ccaaccttgt acctttgagc aacacttcct gtaagtgtcg
    15901 cagctaatgg accgcacatt ggtatatatg gagaaaagat tgccataaaa atatttctta
    15961 tcccacttat aacattagac ctatttgaat tgaaatctat atattcatct tttctatttt
    16021 cactttcaga aagtactaag ttttcacctg ttataaaatc accaaaggca attatgtata
    16081 tcataaaagc cattggtatc ccttgtataa ataacattac acttggaaaa cctattccaa
    16141 aaggagatac tgtatttatc agatttttaa aatctactat gtaaactaaa ttgtcaaaac
    16201 taaatctagg aatttctatt tctttactaa atatacctac tattaaactc actaaaatag
    16261 ctgatactac accatattct cctaaatgaa ataaaaattt attcttaact tttagagtat
    16321 caaatctttc agaaaacatt acaaaacaag caaccaaaat tcctactgtt atagatatag
    16381 gataagaatc aaatctccct ttaggattaa actctgatat tatagtcgat ataccgacac
    16441 ccattagtat tccaccttta atagaatttg gtatcttact aactaacttt ccagctacac
    16501 cagttatacc cattaaaata aatattattc ctaaatctat ttgtaaagct gttaatgctt
    16561 gtattctttc aatgcccatg ctgaacttta acaaatatgc agttgtaaga gttatagaag
    16621 ctgtaatcca gccaggtaca actgggtctc ccataaaaac atgcaaacag tataatattg
    16681 agttaatgat agccatactc catgccactt catagggtaa tcccaaaatt tgtgtactaa
    16741 ggccaattgt gcctaaactt gtcatacttg atattatacc ttgaatagct tctggtttct
    16801 caaatctgta atgtataaat ggaattctta tcttaaacat tccaaatttt atatgaggtt
    16861 gttcccttct agcgagtctt tccataactt cactccacct ttcaataact cttataagtt
    16921 tacaacttta taattgttta taaattatca atgtcattaa aattagtata taacatgcaa
    16981 attatataaa caataaatta aatctataaa attgatagat tttgtgcata tatagaaaga
    17041 ccgcttcatt tttagttact taaatcactt aatagtagtt atttaaatca cttgataaag
    17101 gttttttata aaattcaata gttctattcc atgattcaaa ctgtcctttt gaatttcctt
    17161 ctacagttcc acctgaagaa aaaatccctc cactcatact catacataaa gtctccttta
    17221 gaggcataat atatggaatt ggtaacggat gacccatatt attgtatgtt aataattcta
    17281 cgttatatgg ataattattt tcttcaaaga tttctaatgc ctttaaacaa gcttcataac
    17341 tattccatat gttatcttct tttccagcaa tcataagtat atctgctttg gaattttcta
    17401 tttttattct agcctctttt ctattctctg ctcttttaag acttttctta taagtactat
    17461 taaatccaaa tggaatacct tttgatatat tttttttctg ttcttcaaag aatatatcat
    17521 tgtctacttt gatatatgga aatggctctc ctttatatga ccatgatgaa gtatcatttc
    17581 cactcatcaa tccattaaga gcttggaaac aataaatgtg aggttgtgat actacaacct
    17641 ttttaatttg ttcatatctt gaagcaagta aaaaggccaa ttctccaccc tttgatgtcc
    17701 catgtacaaa tatttcttta gtactggtaa tttcattttc actaatccac tcaaatactt
    17761 tttcaaaata ttctaatgga acctcttcta atttatctgg taacccttct aaagcaaagt
    17821 atgcaactgt tagtacattg aatcctctag atgcaagtgg agctgctaaa aggtctagag
    17881 cgtctaaatt tccatcagaa ccacctagca ttaaaattgt tttattatta gaattttgtt
    17941 tataaaataa ttttcctata aactcatcag atatatattt tgtggtaata tcttctgatt
    18001 taaatattct ctttaattca acttcttttc tttcaataga agtttcaaat atcatattca
    18061 taattattgg tttatcaata gatatatttt ctgcaaaatt tttaccttca gttttagatt
    18121 tttccagcga ataaattaac cccatacaat tatcagcctt ataagtacct tctataggtt
    18181 caactaaatc taaatctact tctccttttt catttgaaca aaacactcca taagatgaaa
    18241 attcttctcc cttgcaccat ggaaattcca ttttcacact tattttaagt ttagtatttg
    18301 gaattaagtc cgaaaccata atcttaagtc tttcatccac aaatgattct acattgtata
    18361 catttacttt cataatctat ttctccttat aataattatt tatgttttat ataaaacagc
    18421 tttatctaat acaacataaa taaattttta aattaagaca ccatattgac tatttcattt
    18481 tatcaacata ttctattttt gtcaacatat tgtctttttt agcaatttaa aattatgtag
    18541 tctatatgaa atccagttga tttttggcta attaaagata ggaatgctat taggtttaga
    18601 atatatatat tcaaaataaa aaaccactca ttttaagaat ggtttttaat ataaactatt
    18661 tgttattaat aaggatattt ttttatttaa atttatataa tcgaacatct aatatttaat
    18721 gtttaataaa attatgcatt tgcaaaagta tatatggcat taactcttgc tacatataaa
    18781 ttctcatcac tacatccaat atatctagca tatttttcaa tcttttcttt tgcattttca
    18841 acatcatttt catcgctctc cattaactca tttatataaa tatccattaa tgacgcttta
    18901 tattctctta tatcatagcc taaaaattca gtcaatctct caatttcctt actaaaatct
    18961 ccatcttctt ttaccaaaaa ttcgtataca tcttccctat atggatttaa agttattatt
    19021 tgttttacta ctttgcattc gtcagccttc ccaataaaac catctttata attattaaat
    19081 aaggcattag atttttttat agattcttca tcaatataag aatgtaccgc agttatacga
    19141 ttttctataa gggcatctac aacagcataa tgaattttaa aacaatcatt gtataaataa
    19201 ttgctcattt tctttattgt cgattctatt tctaagaagc tcgcgtggcc atttttttta
    19261 tttttatttg gtataataaa agttttattc aagttattaa aaagtctctt aaaatctaaa
    19321 tatttttcac aataaacact tttaaaggta ttgatatcta tagttataat tccaaaactt
    19381 actatagtct caattccttt cttaaaagac tcttctatat atttttctcc taaacttaaa
    19441 cctctatcag atatttgctt tagattttga aaactattta aacaatctct ctcaaagttt
    19501 ttagatattt ccaaagcata taatttaaag attttcctta tcttattata attatttttt
    19561 gtatcatcaa atctgataat agctccattt acatcaaaag taatcattga gttttaacct
    19621 ccccattagt ttcatcaata aaataaattt aatataaact ttataatcat aatattaaac
    19681 ttatttatac tataattatt aatttgatga taaaagctat tttccttcta aataaaaaat
    19741 ttgtctaaag tttctaaaaa atttagacaa atcttaggtt attactcttt aaattatttt
    19801 taaattattt ttaaattatt ccaaatacgc caaataatat tggtataaat atagctggta
    19861 acaaattggc tacttttata ttcgttaatt taagtacatt tagaccaagt cctataatta
    19921 ataaacttcc agctgctgtc atttctgtga ctacaggatc acttaaaaat ccatttaaaa
    19981 atgatgctcc cacacatata ataccttcat acaaaaatac agatattgca gaaaacatta
    20041 cacctatacc taaagaagca gtaaatataa ttgaagctat tccatctata acagatttta
    20101 caaataaagt atcatgactt cctgtaagac cactttctaa tgaaccaact actgccatgg
    20161 ctcctataca gaataataaa ctagatgata taaaaccctc tgctatagaa tcttttttat
    20221 ttcctttaga aaatttactt tgtaaaaaag cacccaactt atttacccat ttgtcaatat
    20281 ctattaattc tcctatcaga gcacctacag ctacggatat tattgtcact aatgtgtttt
    20341 ttccttctag tgctcctgat ataccaatgt acaatacaca taaggcaatt ccattcatta
    20401 tagtatcgct tactctttga ggtaatcctc cttttattat aagacctact atacatccac
    20461 ctgcaattgc tagactattt gctattactc caaacatttt ttctcccctc gattagctaa
    20521 ttgaaattta tcctattatt aaaacctaaa catctatact ttgtattgga tattttaaat
    20581 attccaaaaa ttcttcaaaa ccttttctag atatatttac agttttagta tttatgttag
    20641 gatgaaaatt taagtaatct tctttaatta catctttatc tatgtaaagc tcaacttttc
    20701 tttctgtatc atttattaat gaaaaaggat ttacacttcc aggaagaagt ttaagtacat
    20761 tgtatagtct ttcaggtgaa gcaaacgata atctagaact tcctatctta tctttaagtg
    20821 agtttaaatc aacttgtttt tctcctttaa ctacaataag ataatactta tttcctttgg
    20881 catttcttaa aaataaattt ttacattgac acccctttgt tattccatct aaaatgacta
    20941 attgctctgc tgtatataca gcttcatgct ctactacatc atattcaaca tttaatttat
    21001 ccaaaatatc atatatttta gtttcattca tgctagattc attttcatct tgttcttttt
    21061 tattatttaa atcactcata cttttctcct taaacacttc atcataatat cattgtgagg
    21121 tattattcta ctatagctat tgctcttact ggagctccat cagcatcatt atacttaaat
    21181 ggtgctgcta taaatagaaa tttttctgga acattttcta aatttgtaag attttctatg
    21241 attatttttc ctttttcaaa taaaatatga tgcacttcaa atactgatgt gtcatatcta
    21301 tccactgata gcatatctat tcctatgcct tttatattag tattagctat atagtttgca
    21361 gcttcttttg ttaatgtagg ataacctaca tagtactgct ttttatccca aaattttgac
    21421 caacctgatt taaatattat aaaatcacta ttttttattt catcttcata tttttttaga
    21481 tattctaatt ctatattctc atttcccttt tcaagttcca atacataagc tttcccaaca
    21541 aaattttcta tatccaactt atctagggtc tcacctttag tatacatatg tcttggtgaa
    21601 tccatatgag ttccattgtg agaaaataca ctaattaatg tttcttgata accattttct
    21661 tcaataatag ctactttctt gatatctggt ctattaggct ctgcatatac aggcatgtca
    21721 ttatgtgtca catgagttaa atcaaaaacc ttcatcttta tataccccct atgtttttat
    21781 tttctattat atacctaaat tcattttatt tctatcagat tttattccat tatataccat
    21841 actactatat attttttata aacctcaagg gatttaccga aaaatcttgg aaagaattat
    21901 cagtaaatat aacatcttca cccaaaaaac gtttatacct tccttcttct ttctttaaag
    21961 taattctctg tttttgtgaa tgattattaa aaaccattat cttaagagga ggtttttttc
    22021 tattagtctg cactcttttt aacatgtatt ttatgtgcat atcagcttca ggaaatgaat
    22081 atccacaaaa tattaaaagg tcagtatctc ttaaagtttt ttctgtttca ttccaaacat
    22141 cacttaaaaa tatgtttgac atattcttaa aatatgttgg tggcactata ataggttctg
    22201 ttaactcacc acactctaaa cattttgtct ctgaactatt ttcaataagc ttcatgacac
    22261 caccttcatg tggtgtaaga gttacactat tacaaatggg gcaataaagc caattcaatg
    22321 acccatgtat cttatataat ttgattaatg gttcttttgg ttttctccaa tttaactcct
    22381 ctttaaaatt tgtaaactcc acaccataat caagcataat tggcaaatca ttcttatctc
    22441 ttaatccagt aactgtattg tctatatgta tatcataatt tgcactaata aaagtagtat
    22501 caagaagcaa atcattttcc aaaagatttt caactaaaag tttatgatat ttattatctg
    22561 ttctaggtgt attatgaatt gcttcagcca ttagcagtat taaatattgt cttaaaaaac
    22621 gtatactatc acttctgtta tttagatttt ctagtccaaa atttctaaat gcttcttttc
    22681 tttgttctgc taagtctaaa agacctaaaa cttcttcaaa ggtaggaaaa tttgcattat
    22741 ctatattatc ttttactata tcaatattga acatctgttt aaagaaaata tacaattctc
    22801 tattcatttt ggtattataa tttgtgggat ttagtttttt aaaatattcg ctaaaaagtt
    22861 cattttgaat aggtagattc tctgctgctg aagctcctgc tcctaaaaat attgctattt
    22921 tcatatagtt cctcctaata taaatattac tcctattttt atatccatta aatatccata
    22981 tcatttattt tatgatacaa caataaagct gtcttttact agaaaaaatc ctattacaag
    23041 acagccttat attatattaa atctttaata attcgtaaat agaaagagca tatatttgag
    23101 tagcttttat aatatcctct actattaagt attcattagc ttggtgttct gtttctggtt
    23161 ttcctggaaa aatagctcca aatgcaacac aattatctaa agctcttgcg tatgttgctc
    23221 ctccagatga aagtggtgtt ccatcaagac cagtttcttc ttcatatact tttctaagtg
    23281 tcttaacaag aagagtatct tctggaacat agattgaatc caagaaatca tattcttcat
    23341 aatttaaatt gtatttatct gtcattttct ttaactcttt tacaaaatca tcctttttgt
    23401 atgttactgg aatccttaca tctattcctg caaattcttt ttcgttatca atagttactc
    23461 tacctatatt tactgttagt ttacctgata catcatcctt gcagttagga attatattgt
    23521 ttccattagc atcttcacca ataacttctg ctaaaaactt aattatatta gaatctatac
    23581 ctatattatt aagagctata caaagtctag ccactgcatt tataccagta tcacttgctg
    23641 ctgaatggac actctttcca attatacaga ttttattacc ttcaacagta tactcaaaat
    23701 ttaatttatt aagttctttt tttaatttat cagagtattt tccaatatag attgctttac
    23761 ctggaactgc atttaaagcc tttccaactg acaactctat atcacttttg ctatcacaag
    23821 tcaaatgaac ttgtaaaaga cctttctctg catttgttat tgggaatcta gaatctggag
    23881 taaatccata gtttggtatt tcttcattat tttctttata tttatttata catctccaaa
    23941 gattttcttc atcagcccca aaaatgaatc ttatcctctt attaaagtct acatttaaat
    24001 ccataagagc tttcactgca taaattgctg atattgtagg tcctttatca tcttgtgttc
    24061 ctcttccata caatttacca tcttctaaaa cagcttcaaa tggagggtaa ttccagcttt
    24121 ctaaatcacc ttctggaacc acatcaacgt gaccaagtat acctatcatt ttttcacctt
    24181 gtcctatttc tgcatatcca taatatccat ctttgtatac tgtcttaaat cctaaagatt
    24241 tacataaatc taatatacct cttaaagctt tatctacatt ttcaccaaat gggcaatttt
    24301 cagttgcttc agatataaca ctaggtattt ttacactttc ttgtattgaa gaaatcattt
    24361 catcttgtag agaatttact ttttctttaa tttgctcttt catattatca cccttcaatt
    24421 ttcatcataa tatactaata aatattttga ccttttaaaa aaaaaatatg cttatttttt
    24481 cagttttttt ataaattcaa tcgcttttaa agtccccatt tttgaatggt caaatgtaag
    24541 tccaccttgg aaataaacat tataaggagg tcttattgga gcatctgcac ttaattctat
    24601 agaagaacct tgtataaatg ctcctgctgc cattatgact tcactttcgt atccaggcat
    24661 gtcccaagga acaggtttta cataagaatc gactggtgca gcttcttgta taccttcaca
    24721 aaaacttatt acttcatcag cattattaag tctaatacat tgaataatat cacttcttaa
    24781 gtcatcatat tttggtaata catcatatcc taatttttca aaagctcttg agcaaaagat
    24841 agctcccatt acagcctgag atactatata aggcgccaag aaaaaaccct gaagtacatt
    24901 tctagttgtt ccaaaagtaa gaccacattc ttttcctatt ccaggagaag tcattctata
    24961 agatataagc tcaatcaaat cttttctacc agctatatat ccacctgtca atgcaagccc
    25021 accaccagga ttttttatta aagagccagc cataacatct gctccaacat cagtaggctc
    25081 tttggtatct aaaaactcac cataacaatt atcaaccatt actatagctt ctggtttaac
    25141 agactttatt acttctatag cttcttttat atcactaatt gaaagtgatt ttctccaaga
    25201 atatcctttt gacctttgta tcataacgag tttagttcta tcatttattt tgtttttaat
    25261 tccttctaaa tctaagtttc catcctctaa aaaatctaca tcatcatatg taacaccata
    25321 ctctttcaat gaaccttttt cttctcttat gcctataact ccctctaaag tatcatatgg
    25381 tctaccagta acagataaaa tttcatctcc tggtctaact ataccttgta tacataaagt
    25441 aagtgcatgt gtaccattta caattattgg tcttacaaga gcatcttctg tattaaatac
    25501 tttagaataa atttcctcta ttttttcacg accaatatca ttatatccat atcctgtagt
    25561 ccagttaaag tgcatatcac ttagtttaga ttcttgcata gcttttaaaa ctttatattg
    25621 attatattct cttatttctt ttatttcttc aaacttatct tttatttctt ccattatttc
    25681 ttgcgatagt ttaaaagtat catcatctat tccatagtag tcttttaata attcttttgt
    25741 ctcattaagc attttatcac ctgtctcttt attattttat ttaaacatgt aaaggagact
    25801 attttactaa aacaaataaa ttatagttta taagttagta taagtctcct ctcaatcatc
    25861 ttaaattaaa ttaattatct atctgttgtt attattattg ttgttttggt tttgatttgt
    25921 tagattaata tactttccag gttgtatagt tgatacagca tgtttataaa tcatgttttg
    25981 ttgtctatta tccccttcta ttaatattat ataactatca aaccccttta caagcccttt
    26041 aacttgcact ccattaacta aatatatagt aacaggtatc ctttctttcc ttgcattatt
    26101 taaaaacaaa tcttgtaaat ttaaaactgt atttttcatt agatttgtac cctcccaaga
    26161 ttaaattgaa cttctaaaat attcctacct tatattctat ataagttatg acaaattttc
    26221 aatattaagt tgtatactta aaaatttata tattatttta tagaatcttt aatatataag
    26281 attatttcat tttttaattc gtctatattt tcatattggt ctaaatcaaa ccattttgct
    26341 gttttatatc ttttaaacca tgttatctga cgttttgcat accttctaga atctctttta
    26401 attatttcaa tagcttcttc gtatgtatat tctccatcca gatactttaa tatctcttta
    26461 taacctatac cttgcattga tatcatatct tttttaaatc ccatacttaa aagttttttt
    26521 acctcttcaa ccaatccatt tttaagcata atatcaactc tcatatttat tctttgatac
    26581 aaatgttctc tatctctatt tagaactatt attattggtt gatattcttc attaaatttt
    26641 aaatcacttg aaaaatcatt cattttctta ccacttagac aaacttcaag agctcttatg
    26701 acccttttag tattgttttt atggattcta ttagcagatt cttcatcaag ttcttttaac
    26761 ttattatgca tgtaatctat tcccttttca gcaagttgtt tttgaagttc ttctcttaat
    26821 tcattattgg catcagattg tgcaaaatcc atattatata ttaaagagtt taaataaagt
    26881 cctgtgcctc cagttactaa aacacatttt tcctttttat ttatttcatg tatatatttt
    26941 tttgctcttt gttgaaactc tgaaactgaa aatggatagt caggcttaac ttcatctata
    27001 aggtaatgct tcactccttg catctcttcc tctgtaactt ttgcacttcc aatatccata
    27061 tattcatata tttgcataga atcagctgaa attatctctg cattcatatc ttttgctaat
    27121 tttattgaca aatctgtttt tccaacagca gttggacctg taagtattat aagtggtatt
    27181 tttttcataa cattcctttc catatatttt aaatatccaa atattttaca taattctttt
    27241 aaacattttt tctatttctg tcttagatat ttcgaccatt ataggtcttc catgaggaca
    27301 tgtaaatgga ttctcacact tttctagctg ttccaataaa ctttttattt caatatcgta
    27361 tattttatca tttgctttta tagcagacct acatgccata gatgcaaatc tctctccttt
    27421 taaatcataa ttacttgtaa tttcttctat attatcaatt atttgaagta taaatttctc
    27481 ggtttcaggt actccaaata tagtaggaac acatcttacc attatatgat tattgccaaa
    27541 aatttctagc tcaaaaccaa atttcataaa taactccaag ttattttcaa tttgaagcat
    27601 atctacattt gatacttcaa taaccactgg gtctaaaagt atttgcatat ttatatcttg
    27661 tctatagaac ttctccatat atctttcata tagtattctt tcatgtgcag catgttggtc
    27721 caacaaatac atagaatcgt ctttgctcaa aattatataa gtattaaata caactccaat
    27781 tacagaatat ccgtataaag aaaacttgcg ttttgaacta cacaagtatt cttcttcact
    27841 gtccttaata ctatctccta agtaataatt tccttcattt ttaattccat caacttgaaa
    27901 ctcttcttgt atattatcat ttgcactatc ttcacttaga taattaatgt tttttacatc
    27961 atcttctaca cttgcattta ggacttcact tactgattga aattcattta ttggtttttc
    28021 acttgataaa gtaacaactt caatgacttc atccttacct ttagtaatat tactattttt
    28081 aggagtagat tctaataaat cattatttct taatttataa tctgtacttt tttctctact
    28141 gttaatagcg attctaggtt gtgttttttt atcactataa gtagcatatt ttccaataag
    28201 atttgagtgt attaatttaa cctttaaaaa atctctaagt tctatataca cttcttgttc
    28261 tttttcaaat ttaatttcaa gtttattggg gtgtatattt acatctatac atgatgggtc
    28321 tacttctata tttaagaagc acactgcatg tttacctatt ggaattatag acttataact
    28381 ttctgttatt gcatctatga ttattttact ttttacaaat cttttattta tatagatatg
    28441 ttgtaagttt ttatttgaac ggtatatatt gttattacct atatatccat tcattttaaa
    28501 gtgattgcac ttaaactcta catctattat attttccgtt atttcttttc catatatact
    28561 tctaatagtg tttacaagct ttccatcccc tggagtattt agcatctgtt tattgttatt
    28621 tgtatattta aattgaatgt ttggattacc tattgcaagt ttatttatca aatcacttat
    28681 atttatagtt tctgcatgag tagattttaa aaatttctgt cttgctggtg tattaaagaa
    28741 aatatctttt ataatgatgg tagttccatt agtagaacct attggttctt tcgatattat
    28801 ttttccacct tcaacatata ttttagttcc tattatttca tctttagttt tagttgtcat
    28861 ttctaatttt gatacagctg aaatagatgc caatgcttct cctctaaaac caagagagta
    28921 taaatcatac aaatcatcaa tttttttaat tttactagta gcatgtctca aaaatgattt
    28981 ttcaacttca cttgaaggta ttcctattcc attatctgta atcttaatta gactttttcc
    29041 tccatcaatt atatctatgg aaattttatt tgcacctgca tcaatggagt tttcaataag
    29101 ttcttttact acagaagaag gtctttctac aacttcacct gctgctatct tatttatagt
    29161 caaatcatct aggatgttaa ttatattttt catttttaca cctctaagat tttatttctt
    29221 ttgcttttct ttgaagatta taaagagagt ttatagcatc taatggagtc atatttaaaa
    29281 tatccagatt taaaatttct tctgatagta tatctctatt tacactatca aaagagattt
    29341 gagttaaggc tacttctttt acagaatcca cttgattaat tttctcttta tcatcatttt
    29401 gctttattaa agcaacttca tcatttaatg ctttaaattt agaatttaat ttatcataat
    29461 ccttttttat atgtttgtag tcttttacta taagttcatg ttctattttt agagattcat
    29521 atttattctt aaaattactt tgatttacct taactaattc ttcgtccaaa ttactattaa
    29581 ttatattatt gttttcttta tctccattta ttgcaacact gttgtaaaca tgattttttt
    29641 ccaagtcttt aagtatatat tttgctcttt ctatgacttc gtctggtaat ttagctaatt
    29701 tagcaacata tattccataa ctcttgtctg ctccctgagg aattattttt cttaaaaata
    29761 ttattccttc cccatcttct ttgactgcta ttgaataatt tttaacctct ttaaactctt
    29821 cttctaaatc agtaagttca tgataatgtg ttgcaaataa tgtcttacat ctaatatttt
    29881 tttgaatata ttccactatt gaccatgcta aacttatacc atcataggta ctagttccac
    29941 gacctatttc atctaatata actaaacttc tctctgttgc attttttaat ataagggata
    30001 cttcgttcat ctctaccata aaagtagatt gaccttgaga taaatcgtca ctagctccaa
    30061 ctcttgtaaa tattctatct aatattggaa tatcagcaga ctctgctggt acaaatgagc
    30121 ctatatgtgc cataagagct attatagcag tttgtctcat atatgttgat ttacctgaca
    30181 tattaggacc agttattatg ttaataatgt tttctccacg atttaaataa gtatcatttg
    30241 gaacgaaatt ttcctctcct actatatttt cgacaacagg atgtctacca tttcttatat
    30301 caagtttatt attttcattt attgctggtt ttacatagtt atttatatgt gcaactgttg
    30361 caagggacac aaaaacatct atattagcta tagtcttagc cactttttgt attctatcta
    30421 tgtttttata tattgtatct cttatctcta caaatatctc gtattctaaa gatttaatct
    30481 tttcctctgc atgtagtatc ttttcttcta tttcctttaa ttctggagtt atatatcttt
    30541 ctgcattact aagtgtttgc tttcttatat atgtctcatc tagctttgcc tgtttaaagt
    30601 tagcttttgt tatttctata taatatccaa aaactttatt aaagcctatt tttaatgatt
    30661 taactcctgt tttttctctt tccctgtttt ctatttcctt tacaagaaag gctccattct
    30721 tagaaatctc tcttagttcc ttaagttcat cactaaaatc tgatttaatt atgtttccat
    30781 cttttatggt tatcgtaggt tcttctagaa tagcttcatc tattaaattg tatatatcat
    30841 ctaatttatc catttctgaa acatactctt ttaggatttt tgcatttgat aaatttatag
    30901 tatcttttaa attaggtaac ttttctatcg aattttttaa gtgaattaac tcttttggtg
    30961 ttactctctc aaatgctatc tttccacata ttctttctat atcgtatata tttttaagta
    31021 tatcatttag gtcttctctt aatataaagt catcttttat ttcttctatt acatctaatc
    31081 tattttcaat tttagattta tttatcaaag gttcttctac atattttctt aaaagtctac
    31141 ctcccattgc tgtagaagtt ttatcaagta cgtgtaataa tgaacctttt ttcttattcc
    31201 ctcttatagt ttgagtaagt tctaaattag ttctagtaaa catatctaga accatatatt
    31261 ctgatgaatt gtatatattt atattattaa tgttagatgt tatttgtttt tgagtattgt
    31321 atatataatt cagaagaatt gataagcttg actttattag acctttatca tcaaacttta
    31381 gtttttgtag ataaacatct ggaaaatatt cttttaatat atttatatcc aaatagttat
    31441 cactaaaact ttcatttatg tatatattac ttacagtagc tatatctcta agattttcta
    31501 taaagtctaa atcatttata attatttcag ttggatggat ttttgcaatt tcttcaatta
    31561 ccttatcttc atttaaacat gttgcattag tttctcctgt acttatatca acataagtta
    31621 gacctatatt agtaccatcc ttatacaaag ataataaata attattcttt ttattttcaa
    31681 gtaaatttcc atctaaaact gtaccaggag tgatgactct tataacctct cttctaacaa
    31741 ttccttttgc tgtagatggg tcttccattt gttcacctat tgctacttta tatccatttt
    31801 caactaattt tgatatatat gaattggctg agtggaaagg aacaccacac ataggagcac
    31861 gttcttctaa accacaagct tttcctgtca atgctatttc aagagctttt gaagctacta
    31921 atgcatcttc aaaaaacatt tcataaaaat ctccaagtct aaaaaacagt atacagtctt
    31981 tatatctatt ttttacttca agatactgtt tcatcatagg agttaattta ttcatatcta
    32041 tttgcattat caaacctcct tcttttctaa ttaaatatta tatcatagta gatgattatt
    32101 attaacacaa tatataaaat tttgacatat aactaaaaac aatccaaaat gtatactcac
    32161 attttggatt gctaagttta atgcttatta aataaaaata ttttttttac aataaagata
    32221 ttatttcttt tttaagttta atgatatttt caaggttatc ttcatttaac tttctactat
    32281 ctataacaaa ttctttcttt actattgatg gtttttttga aataacataa attctgtttg
    32341 atagcattat agcttcgtca atatcatgag ttataagaag tattgttgaa tttacttttt
    32401 tcttaacatc taaaagccat ttttgcatgg atgttttagt taatgaatca agtgccccaa
    32461 aaggctcatc taaaagcatt atgtcattag aatttataaa agttctcaag aagttagctc
    32521 tctgtctcat tcctccagaa agttctgaag gatatttatc ttcatatccg cttaatccaa
    32581 acacctcaaa ataaggtttt accattgacc tagctttact ctttttttct ttttttaaaa
    32641 ttagtggtag tgacacatta tctattatag ttttatatgg caagagtaaa tctttttgat
    32701 acatataact atacttacca tttatattca ctttgccact atcataatct gtaagtttag
    32761 ttataatatt aaaaatagta ctttttccac atccacttgg ccctaatata gaaacaaact
    32821 caccttcata aacatcaata cttatatctt ttaaaacttc aatatcttta taagtttttg
    32881 atacattttc tatagatact tttacttttt tattgtggta aaaattcatt tgtgtaagca
    32941 tcacttgcct tcatatcctt atctattaat ttatattctt ttaagaatga tgtatagttg
    33001 ttccatacac tatctttcat ttctccccat cgtggtgcat catctttgta cttgcttgct
    33061 aaatattctt gactctttaa agctagtttt tcatctactt caggtgcatg ttttactaaa
    33121 atttttgctg attcttctgg attttttatt gcatattcat aaccttttgt tgtagctttt
    33181 aagaattttt tagcaagctc tggattatct ttaagcacag tttcattaga aattataagt
    33241 ggtgtataat aatccaatct ctcatctaaa tcttttactg gtataaagtt taagtcatat
    33301 ccaattaaat cagctttaac tgcatcccaa ccttcaaata tccatgcaaa atctacagtt
    33361 ttcatagctg caaagaaatc atcttgacca gtattaacta tctttaactt attaaaatca
    33421 gctccatttt tcttcataac tgctttaaaa acagcttctt ctgatggaga gccccaacca
    33481 ccataagttt ttccttcaaa atctttagct gttgttatat tcttttcttt tggtgatgca
    33541 aacccagaag tattatgttg tataacagtt gctacagctt ttattggaag tggatcttca
    33601 ctagtctttg cataagtaac ttgttcttga tagcttatac caaagtcagc ttttcctgtt
    33661 gctactaaag tttctgctcc actttcaggt ggttgtacta tttcaacatc caaaccttct
    33721 tctttatagt aacccttatc aagtgctaca aataatccag tatgatttgt atttggtgtc
    33781 caatcaagca ccatagttac ttttttagaa gaagtatctt tatcattgtt tttgtcttta
    33841 ttgttatttg aagaacatcc agttattgtt gccaatgtaa aaacacttac taaacctaat
    33901 attcctaact ttttaaaatt cataattgcc tccctcaaat acctttaatc tattaatcta
    33961 tgtcttataa caactttttt tacagcctgt acaattccta caaacattaa actaaatata
    34021 actactaata tagtagatgc aaatacttta tcaagtgcat atgcactttt tgacctaagc
    34081 atataaacac caagtccttt cgttccacct agccattctg caacagttgc tgccattaca
    34141 gcatatgttg cagatatttt aagtccagaa aaaaatttat ccattgccat aggaaattta
    34201 aggtgaatga atgtattaac tttatttgag tccatagtct taaataaatt taaataatct
    34261 ttatcaatat tctcaatgcc atctacaaaa cttattaata taggaaaaaa acaagtaagc
    34321 attaccatca atacttttgg cattgttcca aatccaaacc atatcataaa gagtggtgct
    34381 ataattattg taggtatcat ctgtgtaacc aacataatag gataaagaca tttttttatt
    34441 attgatacaa aatccatcaa tattccaatt accaatgata ttataattgc gactataaaa
    34501 ccaagtattg cttctcctat agtaaccaca gcatgcatgg ataaattttg ataatcttta
    34561 aaaaacacat taattatatc tactggtgtg ggtagtatat actgaggaac ttctaaaact
    34621 cctaccatag attgccaaag aataagtata aacaaaaatg ttgctattgg atatatacta
    34681 tctttgactt tatctatgat ttctaagttt ttcatctata gtcagacctc cagctttttc
    34741 atctactcta gctactgtta tgactctttc acatccaaga ttaaaaggga ttttatgtac
    34801 cttttttaat aattcaaaaa cttcatcaaa atcaccttca acagtagtgc tcattgctcc
    34861 tatttcacat ttaagaccac ttttttcaat ttcagcaata caagcatcta ctactttata
    34921 catctcttct tcacctttgt aaggtcttag tggcattact gctatgtctg cgattaccat
    34981 ttaaatcact cctttaaaaa taattaatac ctactaggta tattttgttt tttattaaaa
    35041 aattttatta ctaaaaagat attatattta aataaaagaa aagtcttatc caaaatagga
    35101 taagacttat ttttatctta actggtctcc ctacactggc attatccaaa caggttcaaa
    35161 gggtcgagat aaaatctcct ctcagcacat tagctccccg aaatatatta tatcttttct
    35221 acttgattat actacactta tcatatttta acaaattttt tatttatatt agatatttta
    35281 tccgttcaaa aatatctaat ataaattgta cattattaca ccaaataaat tatcttaaaa
    35341 tggtatttta tcaacatctt ccaaaactag attaactgaa ttttctagtt tttattttgc
    35401 tatttcaagc atatttggat gtatatcaat tccatacaaa tattgtgcta aatttctgtt
    35461 ttcactcatc taatttgcaa tcgttacaga ttacagatta gaaacagttt ttagactcta
    35521 cttatcttta tctacaaaaa atctttcaat aaattcatta ataatattca gtaatctaaa
    35581 aataaacctc tatctggatt tttaagatac aaacctttta ggaagtatct tttgtgataa
    35641 tataaaaatt actcgtcatt atcatcaaaa tcctcagcaa ccaacatttt atataagaat
    35701 caaactctat aatataatta ttttatccaa tttatttttg agaaataaaa tattttttta
    35761 atttttaaat tttttaatat gcaccttgaa aaaagaaacg ttttcctata ttatggtaat
    35821 agtagaataa aactaaatag gcaaaactag agaaatttag tgacgcaaag ctatagggac
    35881 taagacttat gaaatgagtt atgtcagcca gttgccaaag agatattttc tttttgtttt
    35941 tatgaatgtt ttttaggggg atatatgata tatgtttatg gataaaatgg atagatgtac
    36001 acatatacta actgcatata tatgcagttc acgcgattac tgcaatttta tagatacaca
    36061 attaaatgat tttatactag aatatggaga gaatgtggta gaatcttgtt tgcatcaagt
    36121 gatggtattg gtaagcagat ataattaaag aaattttatt ttctagaaaa ccctttttag
    36181 ttttatctac gttttataat tcattaaaga actaattaaa ataagcttca ttatatattt
    36241 ttcatgaagt aaacagttaa gtttatctca tatactgttt accccaatta cataattcta
    36301 ataatataga ttccaatgat ttaccacgtt tagtagacaa atattcaact tttggaggaa
    36361 tagtcggata agaaatcctc ttaataagtc catctctttc aagatacttt aattcttgtg
    36421 acagtatctt atgacttata tgaacaactt ctttttttaa ttgactgtaa cgtattggct
    36481 cttgttttaa aatatgccat aaaataattg ccctccattt acttcctatc acagtcatgg
    36541 taaaaccaat tgaacaatta aaatcttttt gtctttcttc aatatttgga aattcttcaa
    36601 aattattatc tatatccatc tcaatcctct ctttcaaaat agtaagtatc taactaaaaa
    36661 gtgcattctt gtttagcgta ctaattcgta atataatctt gattaaaaaa tagctatgca
    36721 cactcgtgac tttagtcatg ggaggttcag tgtagcatag agccttacat aatactactt
    36781 ttttagatgt atatgtttaa attttaatat agacatatgt tattttatta attttattta
    36841 aacagataat aataaataat aatttatctc aagttttcta tgtagataaa ctaatctaat
    36901 gtttagaaag gattatataa tatgaataaa aaatttcgtc tttatcaagt tgactcatta
    36961 caaaagaaaa atttacaggt aatccagctg gtgtaattac aaatgctgat ggcttatctg
    37021 aatcacaaat gcaaaaaata gcaagagaat taaataattc agaaactgct tttattttca
    37081 ataacttggc taaaaatcat caagaaaatt atgatgtaca tatcagattt tttacaccaa
    37141 ctaatgaggt tcctatatgt ggtcatgcta ctattgctgc ccattatgtt agagcaatag
    37201 aaaaccaatt caataacaaa acagtatttc aaaaaactgg tgctggaata ttaccagttg
    37261 atattataaa aaaagataat gattatgaaa taattatgac acaaggaaaa attgaagtat
    37321 ctccaccatt acaacaaacc tatcaaaaaa gattacttaa tgcatttggt atttaaagga
    37381 agatattcgt acagattgtc ctattgccat atcatctaca ggacattcaa aagttatgat
    37441 tggaataaag gaattatcaa cattaaatag tttatctcca aatttatatg aattatcgaa
    37501 aataagtcaa gaaataaaat gtaatggata ttatattttt acattacata ataaagaaac
    37561 tctacaagtt catggaagaa tgtttgctcc tgcaattggt atttcagaag accctgttac
    37621 aggaaatgct aatggaccct taggtgcgta ccttgtacat tataaagtaa tacctacacc
    37681 caacacagag tttatatttg aagcagttca aggagaagca ataaaacgca aaggcaagat
    37741 gttagtaaaa gtaattattt tagataataa gccagttgaa gtaaaaataa ttggaaaagc
    37801 tgtaattgca ttctcaacaa acatagaaat ctaattatag attccttcta agtaaatttt
    37861 tgaacttttt aatactaatt taataacaaa ctaatttata tacaatcaaa ttaatttttt
    37921 tattatttgt
//
LOCUS       DAMJZE010000019        35995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJZE010000019 DAMJZE010000000
VERSION     DAMJZE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.019"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(359..871)
                     /locus_tag="PC541_000873"
     CDS             complement(359..871)
                     /locus_tag="PC541_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2838346.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1321..1797
                     /gene="purE"
                     /locus_tag="PC541_000874"
     CDS             1321..1797
                     /gene="purE"
                     /locus_tag="PC541_000874"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2838347.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            1803..2501
                     /locus_tag="PC541_000875"
     CDS             1803..2501
                     /locus_tag="PC541_000875"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2838348.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2513..3880
                     /gene="purF"
                     /locus_tag="PC541_000876"
     CDS             2513..3880
                     /gene="purF"
                     /locus_tag="PC541_000876"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2838349.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3874..4950
                     /locus_tag="PC541_000877"
     CDS             3874..4950
                     /locus_tag="PC541_000877"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2838350.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4944..5537
                     /locus_tag="PC541_000878"
     CDS             4944..5537
                     /locus_tag="PC541_000878"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2838351.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5530..7062
                     /gene="purH"
                     /locus_tag="PC541_000879"
     CDS             5530..7062
                     /gene="purH"
                     /locus_tag="PC541_000879"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2838352.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            7073..8323
                     /gene="purD"
                     /locus_tag="PC541_000880"
     CDS             7073..8323
                     /gene="purD"
                     /locus_tag="PC541_000880"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2838353.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8348..12154
                     /locus_tag="PC541_000881"
     CDS             8348..12154
                     /locus_tag="PC541_000881"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2838354.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12360..13232
                     /gene="rfbD"
                     /locus_tag="PC541_000882"
     CDS             12360..13232
                     /gene="rfbD"
                     /locus_tag="PC541_000882"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2838355.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13341..14210
                     /gene="rfbA"
                     /locus_tag="PC541_000883"
     CDS             13341..14210
                     /gene="rfbA"
                     /locus_tag="PC541_000883"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2838356.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14259..14816
                     /gene="rfbC"
                     /locus_tag="PC541_000884"
     CDS             14259..14816
                     /gene="rfbC"
                     /locus_tag="PC541_000884"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2838357.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14854..15837
                     /gene="rfbB"
                     /locus_tag="PC541_000885"
     CDS             14854..15837
                     /gene="rfbB"
                     /locus_tag="PC541_000885"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2838358.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15867..16535
                     /locus_tag="PC541_000886"
     CDS             15867..16535
                     /locus_tag="PC541_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2838359.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16544..16972
                     /locus_tag="PC541_000887"
     CDS             16544..16972
                     /locus_tag="PC541_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2838360.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16996..17310
                     /locus_tag="PC541_000888"
     CDS             16996..17310
                     /locus_tag="PC541_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2838361.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            17461..17736
                     /gene="flgM"
                     /locus_tag="PC541_000889"
     CDS             17461..17736
                     /gene="flgM"
                     /locus_tag="PC541_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2838362.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17741..18157
                     /locus_tag="PC541_000890"
     CDS             17741..18157
                     /locus_tag="PC541_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2838363.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18175..19485
                     /gene="flgK"
                     /locus_tag="PC541_000891"
     CDS             18175..19485
                     /gene="flgK"
                     /locus_tag="PC541_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2838364.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19504..20436
                     /gene="flgL"
                     /locus_tag="PC541_000892"
     CDS             19504..20436
                     /gene="flgL"
                     /locus_tag="PC541_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2838365.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20452..20844
                     /locus_tag="PC541_000893"
     CDS             20452..20844
                     /locus_tag="PC541_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2838366.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20838..21050
                     /gene="csrA"
                     /locus_tag="PC541_000894"
     CDS             20838..21050
                     /gene="csrA"
                     /locus_tag="PC541_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2838367.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            21072..21467
                     /gene="fliS"
                     /locus_tag="PC541_000895"
     CDS             21072..21467
                     /gene="fliS"
                     /locus_tag="PC541_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2838368.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            21467..21823
                     /gene="fliS"
                     /locus_tag="PC541_000896"
     CDS             21467..21823
                     /gene="fliS"
                     /locus_tag="PC541_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2838369.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21844..23367
                     /gene="fliD"
                     /locus_tag="PC541_000897"
     CDS             21844..23367
                     /gene="fliD"
                     /locus_tag="PC541_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2838370.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23370..23705
                     /gene="fliT"
                     /locus_tag="PC541_000898"
     CDS             23370..23705
                     /gene="fliT"
                     /locus_tag="PC541_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2838371.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23807..24679
                     /locus_tag="PC541_000899"
     CDS             23807..24679
                     /locus_tag="PC541_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2838372.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24772..26913
                     /locus_tag="PC541_000900"
     CDS             24772..26913
                     /locus_tag="PC541_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2838373.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26937..28814
                     /locus_tag="PC541_000901"
     CDS             26937..28814
                     /locus_tag="PC541_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2838374.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28807..30921
                     /locus_tag="PC541_000902"
     CDS             28807..30921
                     /locus_tag="PC541_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2838375.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYSSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30930..31925
                     /locus_tag="PC541_000903"
     CDS             30930..31925
                     /locus_tag="PC541_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2838376.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            32017..33249
                     /locus_tag="PC541_000904"
     CDS             32017..33249
                     /locus_tag="PC541_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2838377.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33269..34213
                     /locus_tag="PC541_000905"
     CDS             33269..34213
                     /locus_tag="PC541_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2838378.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34307..34714
                     /locus_tag="PC541_000906"
     CDS             34307..34714
                     /locus_tag="PC541_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2838379.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34801..35892
                     /locus_tag="PC541_000907"
     CDS             34801..35892
                     /locus_tag="PC541_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2838380.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        14746 a         3620 c         6551 g        11078 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaata aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
      181 tatttgaaat aaataaaaat attgaaggat ataatattca actggtatat aaataataaa
      241 tgatagttaa attttacctt agaggctaat taggtgttta gtcaaagcta atgcctttag
      301 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
      361 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaactcat
      421 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt aatggtaagt
      481 ctaaagtttg tctaacttta tttaccactt tatttttagg aaatactcct gcccaacaag
      541 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
      601 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
      661 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctgtttc agcagttcct
      721 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
      781 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
      841 ttgaacgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
      901 tatttatata atatcattac tttaaatttt agctattact tatataaaga ataaactttg
      961 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
     1021 agtattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattgg
     1081 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
     1141 acataaataa agttcgatac gtagcgaatg aaaaaattac aagaaatatt ttttttaaaa
     1201 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
     1261 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
     1321 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
     1381 aaactgcttg aaaaatatgg aatagaagtt gtagcaagag cattatcagc tcatagaaca
     1441 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
     1501 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
     1561 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
     1621 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
     1681 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
     1741 tatagagaag agatggcaca aaaggtgtta gaagacgaca ataatttaag ggggtagcag
     1801 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
     1861 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
     1921 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
     1981 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
     2041 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
     2101 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agctacaaaa
     2161 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
     2221 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaatgag ttactaaaag
     2281 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaggatt
     2341 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
     2401 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
     2461 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
     2521 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
     2581 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
     2641 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
     2701 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
     2761 taattgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
     2821 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
     2881 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
     2941 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
     3001 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
     3061 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
     3121 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
     3181 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
     3241 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
     3301 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
     3361 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
     3421 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
     3481 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
     3541 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
     3601 aagagaagca ggtgcaaaag aaattcatct tagaataact tctcctccag tagcttactc
     3661 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
     3721 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
     3781 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
     3841 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
     3901 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
     3961 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
     4021 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
     4081 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
     4141 aatgatttaa tttgtcaagg agcaaaacca ttattcttcc ttgactatat agctcttggg
     4201 aaattagtgc ctgagcatat agaaaaaata gttggtggaa ttgcagatgg ttgcaaaatg
     4261 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
     4321 gactatgatt tagcaggatt ttctgtggga atagcagaca aagaaaagat tgtttctgga
     4381 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
     4441 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacgatat
     4501 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
     4561 aaattagctt tagatgtatt agctaaacat gacataaaag ctgtagctca cataactggt
     4621 ggagggctta tagaaaatat aacaagagtt atacctaaag gattaggact agatatcaat
     4681 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
     4741 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
     4801 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgattat
     4861 gtagataaaa aatatagtga attattagaa gataaagctt atataattgg tgaagtagta
     4921 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtgggggaa
     4981 caaatttaca agcagttata gatggtactg agtctggaga aataaaaggt caggttaaag
     5041 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
     5101 aagcaatttg tgaaactgat gaagacaaga taatagaaat tcttaaggaa aataaaatag
     5161 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aatgagttta
     5221 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctggattct
     5281 atggagaaaa agttcatcaa ggtgttatag attacggagc taaagtaact ggtgctacag
     5341 ttcattttgt tgatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
     5401 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
     5461 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
     5521 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
     5581 tttgctaaag agttaaataa attagattat gaaattatat ctacaggaaa tacatttaaa
     5641 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
     5701 atattagatg gaagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
     5761 agagataaag aatctcatgt agaaactgta aatgaacata aaatacattc aatagattta
     5821 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
     5881 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
     5941 aaggatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
     6001 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
     6061 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacgtatcct
     6121 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
     6181 caaaatggtt tcttatattc acagtcaaat gcaaaaaatc caatcttaaa ttatgagcaa
     6241 ttaggcggta aagaactttc ttttaacaac ttaaatgatt tacatggatg tttagaagta
     6301 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
     6361 tgtggggtag gtttaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
     6421 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
     6481 gagaaattaa atgaaatatt cttagaaata gtagtagcat atgattttga accagaagct
     6541 ttagagatac ttaaacaaaa gaaaaatcta agaatattaa aattagctaa aatagaaaat
     6601 agtttacaac catatgaaat gaaatattta gatggaaaat tattgataca agatagaaac
     6661 aatatattag cagaaaaaag tgaaaatgta acaaaggaaa aaccaacaga tgcacaactt
     6721 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcaata
     6781 gtaaaaaatg gacaaactct agctttaggt tgtggacaaa catcaagaat ttgggcatta
     6841 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagac
     6901 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
     6961 gttcaaccag gtggctctat aaaagataaa gattcaatag aagcttgtga caagtatgat
     7021 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
     7081 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
     7141 aaatgtagaa aaaatttatt gtgcaccagg taatgcagga attgcaaacg tagcagaatg
     7201 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
     7261 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
     7321 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
     7381 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
     7441 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
     7501 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
     7561 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
     7621 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag ataatgatac
     7681 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
     7741 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
     7801 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
     7861 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
     7921 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
     7981 tttacatgag ataatggaag ctatactaga taataagtta aaagatatag aaataaacta
     8041 tagtgatgat gaagctgtat gtgttatgct tacatcagga gggtatccag acagttatga
     8101 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
     8161 tggcactaag atgtttgatg gtaatttggt taccaatgga ggaagagtaa ttggtataac
     8221 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
     8281 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
     8341 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tagttgaaaa
     8401 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
     8461 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
     8521 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtagtata
     8581 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
     8641 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
     8701 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
     8761 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
     8821 aaaaccagaa acgttaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
     8881 atttatagat ttagatgaaa atggattaaa aacatttgta agtgaaaaag gattagcaat
     8941 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
     9001 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
     9061 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
     9121 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
     9181 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaaa atgggaaatt
     9241 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
     9301 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
     9361 tgaaatagaa ccatttggtg gagcagctac ttgtttagga ggagctataa gagacccatt
     9421 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
     9481 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
     9541 tcatggatat agttcatatg gtaaccaaat aggtcttaca actggacaag ttgcagaagt
     9601 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagcacc
     9661 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatcgttc ttcttggagg
     9721 aaaaacagga agagatggct gtggaggagc aactggttca tctaaagaac atagtgaaga
     9781 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaacg aaagaaaaat
     9841 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
     9901 agcaggtgga gtttgtgttg ctataggaga gattgctgat agcttagata taaacttaga
     9961 cttagttcct aaaaaatatg atggacttga tggaactgag cttgcaattt cagagtcaca
    10021 agagcgtatg gcagtagcca taaaaaaaga aaataaagat aaatttatac aattagcagt
    10081 agaagaaaac ttagaagcta cgcatgtagc tacagtaaca gatacaggat atttaagaat
    10141 gttctggaat ggaaaagcaa ttgtagatat aaatagagag tttttagata caaacggagt
    10201 aaaacaaact actgatgttc atgttactaa agtagatgaa gaaaatacat tctttagctc
    10261 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtact
    10321 atctgacctt aatgtttgtt ctcaaaaagg attagtagag atgtttgata atacaattgg
    10381 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    10441 ggttgctaag ataccagtac ttggaggaga aactaatact tctacaataa tgacttatgg
    10501 atacaatcca aaggtaggaa aatggagccc attccatggt gccttgtatg cagtagtaga
    10561 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    10621 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    10681 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    10741 tatgtctggt acatttaaag atattgaagt tccaccaaca ttagtttcat ttgctgttga
    10801 cacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    10861 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    10921 cttagataaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    10981 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    11041 gtttagtgaa gaggctgaaa aagcatttac agatgataaa ttatttgagg ctagttatgg
    11101 aaatatagta cttgaattag caaatgatga tttaagtatg ttagaaggat acaattatgt
    11161 tgtgcttgga agtacagtta aagaaactag tatatttatc aaaggtgaag aacttgcatt
    11221 agatgaatta tataaagcac attgtagtac tttagagcct atattcccaa ctaaaacaga
    11281 agaagtaaaa tcaaagatag aaactataag ttatatatca caaggagaag ctaaaaaatc
    11341 atcattaagt atagctactc caagagtatt tataccagca ttccctggaa caaactgtga
    11401 atatgactct gcaagagcat ttgaaagagc aggagctaat gcaagtataa gagtatttaa
    11461 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    11521 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    11581 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    11641 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    11701 agggttagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    11761 caataatatt ggtcgccatc aagctaagat tgctagaact agaatttcat ctaataaatc
    11821 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    11881 aggtaagttt gttgccagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    11941 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    12001 ttcattctat gctgttgaag gtataactag tgctgatggt agagtgtttg gtaaaatggg
    12061 acattctgaa agaattggtg aggaagtata taagaatatt ataggtgaaa aagaccaaaa
    12121 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    12181 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    12241 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    12301 gctgtgttat aattaattat taagtaaaat tgaatattta ttggaggaca acataattaa
    12361 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt aaccaattag
    12421 aagcaatcaa tcaaagtata aaccaaccaa aatatgttat attagctaca acaagaagtg
    12481 atttggacat atccaatcag actaatgtag ataattttat tttgcataat aaacctgatg
    12541 tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    12601 catacaaaat aaatgcttta ggagtgagaa atctagcaat agcatcagaa aaagttaatg
    12661 ctaagcttat tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    12721 gggaagacaa taaaactgaa cccaattctg tatatggaaa aagtaaactt atgggagaaa
    12781 aatttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    12841 atgggaataa ttttgtaaag accatgataa aactttcttt agaaaataaa gaggttaatg
    12901 tggttaatga ccagtttgga actccaacaa gtacagtaga cttagcaaaa attataataa
    12961 aaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    13021 ggtatgattt tgccaaaaag atatttaaac taaaaaatat agatataaaa gtaaatccaa
    13081 ttaagagtaa cgaatttaaa tgtaaggctc caagaccact atattctgta ttggataatt
    13141 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    13201 atttagaaag atatgatata aatgaaaagt aaatgtatta ttttgagaaa agaaaataag
    13261 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    13321 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    13381 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    13441 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    13501 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    13561 acataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    13621 aagaatttat aggaaatgac agagtgtctc ttgtattagg ggataatata ttttatggat
    13681 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    13741 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    13801 tgtcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    13861 tttatgataa tgatgttgta gagatagcaa agctagttga accgtcagat agaggtgaat
    13921 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    13981 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    14041 attatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    14101 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    14161 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgtaa tatggtagat
    14221 aaaatttatt ataaatttta ttaagggagt ttattataat gggaaaaatc aatgttataa
    14281 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttatgat gaaagaggat
    14341 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgtttta
    14401 ttcaagataa tgagtctagc tcaagaaaag gcgttttaag aggaatgcat tttcaggtta
    14461 aatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    14521 atttaaggaa agattcacaa acatttggga aatgggaagg tgtaatattg tctgataaga
    14581 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    14641 aagcagtatt tagttacaaa tgtacaaatt attattaccc agaatttgag aatggcttta
    14701 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    14761 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtacaaga cgttaataaa
    14821 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    14881 gcaggattta ttgggagtaa ttttatacat tatatgctta acaagtatgt agattatttt
    14941 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    15001 gacaattcca attataaatt tgttaaagga gatatttcaa atagggagca ggtgtataag
    15061 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ctgctgaatc tcatgtagat
    15121 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    15181 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    15241 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc ttctatcaat
    15301 ccaagttctc catattcagc ttctaaagca tcagcagatt tattggtaag ttcttattat
    15361 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    15421 tttcctgaaa aattgattcc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    15481 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    15541 atagacttaa taatacataa aggaaatata ggggagattt acaatatagg aggtcataat
    15601 gaaagaagta atctagaagt agtaaaaatg atattaaatc ttttaggtaa atctgaagag
    15661 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    15721 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    15781 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtataactt
    15841 taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt aatatattag
    15901 cattgactca attatctggt agtaatacta caaatcaatg taattgtaca tataataatt
    15961 catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa aataatcaac
    16021 atacttctaa tagctacaat ttattgtcta attctgagaa tttatttaat gaacttgata
    16081 atgtagtaaa taataccact agtagattta tagatgttga tacaaaagat aaaaatgtaa
    16141 agttgagaat tgaaaatgct gtaaatcaag catcaaaaaa atataacgta gattcaaatc
    16201 ttataaaagc aataattaaa gttgaatcag attttaatcc aaataccgtt tcttctgctg
    16261 gtgctaaggg tcttatgcaa cttatgccag aaaactgcag agacttaggg gttacagacc
    16321 cttttaatat agaacagaat atagatgctg gaactagaca tattaaggaa tacattgata
    16381 tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct ggaagaatga
    16441 aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa acacaaaatt
    16501 atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggaa taaatataga
    16561 taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag agagtaataa
    16621 attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata taaaacgaaa
    16681 tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact atggcgatat
    16741 tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata atatgtattc
    16801 acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa agattgttga
    16861 tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa acatagatat
    16921 tgtttctact gttgacatga tacaaggctt attgatagat atgtatagat aaggttatac
    16981 aaggagaaaa attttatgaa tagcaaaaaa gaggatatat atgaattgaa ttttgggcaa
    17041 ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa tgcaaaagta
    17101 aaggtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt agatttaaaa
    17161 gaaggctcta taataaaatt aagtaagaat gtggatgaaa aattagatat atatgcaaac
    17221 gatagattgt ttgcttatgg agaaagcatt attacaaatg agaagttatc tgtaagatta
    17281 tctaaaatag aagattcaga tgtaaattag cattttaaat atatcccttt agtctattct
    17341 gtatgagttt agctaagggg atatttttat aaaaaattta aaatatgtgt gaattatata
    17401 aatattttta ttttatgagc gataactaaa ttataagtat gtaattttgg aggtgaaact
    17461 atggatatta aaaatgtaag ttcaaatatt gtagtgaata attatttaaa gaatacatct
    17521 ggtataaaaa aagtggaaga ggttaaaaaa attaataaaa cagacaagat agagttatct
    17581 aaatttgcaa aggaaatggc aagtgtgtca aatatgagtg atgagaatat tagaaataaa
    17641 aaagtcgatg aaataaaaaa tgcattagaa aatggaactt acaaaataaa accaaaagaa
    17701 attgcgaaaa aaatgataga ggatatgcga ggataaagtc atgattacag acttaaaagt
    17761 tataatatat gaagaaaaaa atataattag taagatgatg gaattattaa aagaacaatt
    17821 tgattatata gttgaaaaag acttagaaaa tttaaatatg ttaaatccta aactagaaga
    17881 tattagcagg gagcttgcat ccatagaaat aaagagaaga caactttttg gtaatgatat
    17941 ttcaatgagt gaagttgttg aaaatagtaa tgatggatat ttaaaagaaa tatatacaga
    18001 cttgaaacgc attcttgctt tagctaaaaa tcaacaagaa tcaaatgatt ctctaataaa
    18061 gaaagaactt atttttacga aaaaaatgat taatttcata aaaccagctg ataagcaaac
    18121 tacaacttat aattcttatg gaaatattag aaaataaata ttaggaggag aaatatggca
    18181 ggattatttt ccattttaaa tacagctaat tcaggaatga atgcgcacca aaaagcaata
    18241 cagacaattt ctcataatat aagtaatcta gatacagacg gttattctag gcaaaggacc
    18301 gagtttgcta caaatagtcc aatgtatatg cctagtttga gtaattcaat tgcaagagga
    18361 caattaggga ctggagtaca tgtaacggat gtaacaagag ctagaaatag tttttatgat
    18421 taccaattta gggcagaagc tcataagtat ggtaagacag tttcaaaata tgattattac
    18481 aatactatag aaactatact aaacgaacca tcagactatg gaatatctgc aggtatagat
    18541 gattttttta aaggatggga gtcattatca aaggatataa atagtgacag taaaaaaagt
    18601 ctagtggtag aaaacgcatc taatttagca actttaataa gtgaaagtta taagaagctt
    18661 gataagttaa aagaagatat agattctaat atagacactg agataaaaca aataaatgac
    18721 atgttaaaaa gtcttgaaga tttaaataaa tctattgata ttatatctgg ttctggctct
    18781 acaccaaatg atttgcttga tgaaagagat agaattttag ataacttgag ttttaaacta
    18841 gactttgaaa atagtgatgt taaaaatatg cttgcagatg gtaaattaga acttaatgaa
    18901 ttaaaaaatc cagatggaac ttggaaaaca ggtatatcag ggaccttaca aggcttgttt
    18961 gaaatgcatg gtaagattga tacatataaa agcgatttaa aagatgtatc tgatggatta
    19021 gctaaacaga taaatgatgt ttataatacg tctgcaggaa taacagtaac agattttttt
    19081 ataacttcaa atgtagatgg tgaagatatt ataaagataa atccagctat aaagagtaac
    19141 ccaaatgaat taaagcttac tgctgatgaa gcttcaaaaa ttgctaagtt aaaggatgaa
    19201 aaaatagata ttggggtggc tggtggaaaa gtaagtacaa taagtgacca ctataaagcc
    19261 tttgcagaat caatcggttt agattctcaa aaagtaaatc aagatgaagc aaatcaacgt
    19321 aaaattataa ataatattga taactcaaga atgagtgttt caggtgtatc tttggatgaa
    19381 gaaatgacag agcttataaa aatacaacgt tcatatcaag ctagtgcaaa agttatgtca
    19441 acagctgtgc aactactgga tgttgtaata aatggaataa tttaatagaa gggggaattt
    19501 ataatgagag tcagtacagg tatgatgtct agcagctatc taaactcact acaagataat
    19561 ctacaaagat tggataaggt taatagacaa gttaatacaa caaaagaaat aaacaagcta
    19621 tcagataatc catataaagc aataaagata ttaaactcaa aaagtgaaat aaagactatg
    19681 gagacttatg ttgagaattg taaagataca gggaattggt tagaaactac tgatactgca
    19741 ttagatcagc ttggaaaatt attatcagac ataaagaaaa ctttggtggc aagtggtgat
    19801 ggtgcatact cagatgatga attaaagact cttagtaatg aaactaatga aaaaatgaaa
    19861 gaaattgcaa atgctttaaa tgcaacacat gaagggaagt acatattttc aggttcaaat
    19921 acgggggtac cacctgttga atgtatagat aatgctgatg gttcagtgag tttaaagttt
    19981 aattctagtc taaatcttaa taaattaaat gactctttga gtatggatat agctcaagga
    20041 ataagtgtag attataacgt taaattgagt agccttggat ttgacccaac aaaaactgac
    20101 ccatttgaag agttaaataa catatcaaaa aaattagcaa atccagatgc tgatgctata
    20161 aaagagctta caacaacttg tttgggcgat atggaacaac taatagaaaa tacagtaaat
    20221 gttaggtcta tatatgggac aaaagcaaat acagtagagg ctatgaagga aaaaaacgat
    20281 gatagtttga tacagttaaa ggatattttg tcacaaaatg aagaaataga ttatggtgaa
    20341 aaacttgttc aattaaaggc agcagagtta acttatcaag cttcactaca aacaggagga
    20401 aagttattta atgtatctat attagattac atataataaa ataacgcaga tatgatgaag
    20461 gttacattaa aaaaaggtat attaggtttt gaaaatttaa aagaatatga gcttttagat
    20521 atagaagata atgattattt aaaagaattt aattctactg aagaagaagg tataggattt
    20581 gttgttgtat ctccatttga gattatcaat gaatatgaaa tagttttaaa tcaggaaaca
    20641 atagaaaaac tagaagtgaa atctccaaag gatgttgtat tatttaatat aattactctt
    20701 ggtcagacat ttgaagaatc aactgtgaac atgaaagctc ctatagtgat aaatataaaa
    20761 actaattttg gaatgcaaat aattttacag gatgataaat attcaataag gcatccatta
    20821 ttgagaggtg ataatggatg ctagtaattt caagaaaaaa agatgaagca gtacttatag
    20881 gagaaagcat agaagtaaaa attgtaggag tagatggaaa caatgtaaaa ctagctatat
    20941 cagcgcctaa caatataagt atattaagaa aagaaatata cgaaaaagta aagagtgaga
    21001 atataaaagc tacaaataaa aatataaaaa ttttaaagtc attaaaataa taagcttgga
    21061 ggaagtaata tatgtatgga gaaaatcctt ataattctta taaacaaaat gctgttttta
    21121 tggcatcaaa ggaacaattg ttacttatgc ttgttgatgg agcagtaaaa tataccaaaa
    21181 tagccagagc agctatctta gataaaaata cacagaaagc gcatagagaa cttgtaagag
    21241 ttcaggatat atttacagaa ctaatggtta cattagacca aaatgcagga caatgggcaa
    21301 aggatatgta caaagtttat gattttgtaa gatatgaact ttcaagagcc aatattagaa
    21361 aagacatcca ggtaatagat aatgtgttac ctgtaataga acaaattaaa gatacatggc
    21421 atgaagctga taaaaaaagt aaagaagaaa gaagtaggta caaataatgg aattaaattt
    21481 agctgaatta gataagttat caaaagagga attactattg atgcttgtag atggcacagt
    21541 aaaatatacc aatatatcca aagaagctct tttaaataac gactatttga aagcacataa
    21601 tgaacttgta agagttcaaa atatatttac agaactaatg actacattag accaaaatgc
    21661 aggacaatgg gcaaaggata tgtataaagt ttatgaattt ataaaatctg aattagcaat
    21721 agctgatgaa aataaagata ttaaaataat tgatgatata ttgccaatag taaaacaaat
    21781 tagagataca tggcatgaag tttacaatca actaaaaata taattagatg gagggataaa
    21841 ggtatgtcaa gtataagtcc tataagagtt acaggtcttt caggaaattt tgatatggaa
    21901 ggcataatcg aagctagtat gattagagac aaggaaaaag ttgataaagc aaaacaagaa
    21961 caacaaatcg ttaaatggaa gcaagaaata tatagaaatg ttatacaaga atcaaaagat
    22021 ctttatgata aatatctaag cataaattct cctaatagta tagtaagtga aaaagcatac
    22081 tcttctacaa gaataaccag ttctgatgaa agtattatag tagcaaaagg ttcagctggt
    22141 gcagaaaaaa taaattatca atttgcagtt tctcaaatgg ctgaaccagc aaaatttact
    22201 attaaattaa attcaagtga acctattgtt cgacagttcc ctccaaatgc cagtggagcg
    22261 agttctttaa ctataggaga tgtaaatata ccaatatctg aacaagatac tacaagtact
    22321 attgtaagta agataaactc cctttgcgca gataatgata taaaggcttc ttatagtgag
    22381 atgacaggtg aattgattat ttcaagaaaa cagactggtt cgtcatcaga cattaattta
    22441 agagtaattg gaaatgacaa tttagctcag caaattgcta atgataatgg tatcacattt
    22501 ataaatgatg ctggtggaaa caaagtggca aatgtatatg gaaagaatct agaagctgat
    22561 gtaactgatg aacatggaag agtaactcat ataagtaaag aacaaaattc atttaatata
    22621 gataatattg actataatgt aaattcaaaa ggaactgcaa agttgacttc tgtcactgat
    22681 actgaagaag ctgttaaaaa tatgcaagca tttgtggatg attataataa actgatggac
    22741 aaggtctatg gtttagttac tactaaaaaa ccaaaagatt atccgcctct tacagatgcc
    22801 caaaaagaag atatgacaac tgaagaaata gaaaaatggg aaaagaaagc taaagaaggt
    22861 atacttagaa atgatgatga gttaagaggt tttgttgaag atattcaatc tgcatttttt
    22921 ggagatggaa aaaatattat tgcattaaga aaactaggta tcaatgaaag tgaaaattac
    22981 aataaaaaag gtcaaatatc atttaatgca gatacttttt caaaggctct tatagatgat
    23041 agtgataagg tatacaaaac actagcaggt tattcttcga attatgatga taagggaatg
    23101 tttgaaaagc taaaagatat tgtatatgaa tattctggaa gttcaacttc taaacttcct
    23161 aaaaaagcag gtatagaaaa aactgcttct gctagtgaaa atgtatattc aaaacaaatt
    23221 gcagagcaag aaagaaatat aagcaggtta gttgaaaaaa tgaatgataa agagaaaaga
    23281 ctttatgcta aatattcagc cttagaatct ttgttgaatc agtattcttc ccaaatgaat
    23341 tatttttcac aagcacaggg taattaatta tggataaatt aaaagaaaaa ttaaatttat
    23401 ataaagatat atctttacaa attataaatt tgattgaaaa ggaagaatat ataaagataa
    23461 gtattaagtt aggtgaaaga caagagatta taaatagtgt aagtgaaatt gatagaaatg
    23521 attttataca gctttataat agaatggaat taattgaaat tgatagcagg ataagagata
    23581 ttttacaagg acaattatta gaagtaaaaa aagaattaca tgagtataag cttactaaac
    23641 aagtgaacac aatgtattat aacttaaata gagaaaaagt aaatattttt aataaaaaag
    23701 tataaagtta tgtaccatct tgtccgatta tataaatgaa gaattaataa aaaagattaa
    23761 aagtagaaat gacaaggatg tcaactatac taaggagggt aaaataatga gagttaatac
    23821 aaatgtaagt gctttgatag caaataacca aatgggaaga aacgtaaatg cacaaagtaa
    23881 gtctatggag aagctatctt ctggtgttag aattaagaga gctgctgacg atgctgctgg
    23941 gcttgctata tctgagaaaa tgagagctca aataaaaggt ttagaccaag caggaagaaa
    24001 cgttcaagat ggtatatctg ttgtacaaac agcagaaggt gcattagaag aaactggtaa
    24061 catactacaa agaatgagaa ctttatcagt acaatcttca aatgaaacta atactgctga
    24121 agaaagacaa aagatagctg atgagttact acaattaaaa gatgaagttg aaagaatatc
    24181 aagctcaatt gagtttaatg gtaagaaatt acttgatgga agttctacag aaataagatt
    24241 acaagttgga gcaaactttg gaacaaatgt tgcaggaaca tctaacaaca acaatgaaat
    24301 aaaagttgct ctagtaaata cttcaagtat aatgagtaaa gcaggtataa caagttctac
    24361 aatagcaagc ttaaatgcag atggaacttc tggaacaaat gcagctaaac aaatggtatc
    24421 tagcttagat gtagctctta aagaattaaa tacatcaaga gcaaaattag gggcacaaca
    24481 aaatagacta gaatcaactc aaaacaactt aaataatact atagaaaatg ttacagcagc
    24541 tgaatcaaga ataagagata cagatgttgc ttcagaaatg gttaacttat ctaaaatgaa
    24601 tatattagtt caagcatcac agtcaatgct tgctcaagct aatcaacaac cacaaggagt
    24661 tttacaatta ttaggataaa ataattttgt atattggata tagaagaaag ggtaaggctt
    24721 tgctttactc ctttcttttt ttacaaatat acttttgaaa ggaaaattta tatgttactt
    24781 agtatagtta tgattgttaa aaatgaagaa aaaatacttg aaaaaacttt aaaatcatta
    24841 accacattaa gaaattctat agaaagtgaa cttataatag tcgatacagg ttctacagat
    24901 gatactataa aaatatctaa gaagtacacg gaaaaagtgt attttcataa ttggaataat
    24961 gatttttcta gtatgagaaa tatatcaata tcctatgcta aaggtgaatg gttacttata
    25021 ttagatgcag atgaaatact aattgattct tcaactattg taaaattttt taaagatggt
    25081 cttgataaaa aatttaattc tgcttcaata caattaaaaa atctttactc atatgataaa
    25141 aaattatatg gatattgttc agtgcttaga ctttttaaaa acgttgattt tagataccat
    25201 ggtaaagtac atgaacaacc aatatacaaa aaccctatat ttaataatgt tgctgatttt
    25261 gaacattatg gatatttgtt tgaagatgaa gaaataagat taaataaggt gaaaagaaat
    25321 gaaaaactat tatttcaaga gttaaaagag aatgataatt ctccatatac aaactatcaa
    25381 ttagggaaaa attttattat attaggaaaa tatcaggagg ctttatacta tcttgagaaa
    25441 tcttatgaat tatattctaa actagaattg gcacctggtt acttgataac aagccttgta
    25501 aaaacatatt tatatttagg aaaacataag aagtgtgaaa gattgtgctt aaagtatatt
    25561 aagaaagaca tgaataatat tgatatatat tattatattg cacaagcaca agtagattta
    25621 ggaaagtatg aaaatagtat agattcgtat aagagatata tttacttgtt agataattat
    25681 gaaataagta cacaagcaaa tagtttattt tcagacactg atactgtagg gcttagagat
    25741 gaggctatta ttacactgat aaagatttat tataagcttg aaaaatatga gtttgtaatt
    25801 tcagagtata ataacattga agatgtggaa aagaaaaaga atgtgtactt tagcttattt
    25861 atgtcactat ataaattgaa tagatttgaa gatataaaaa attattataa agaagtttca
    25921 aattctaaaa ttgaaaaaaa tagtttttat agaaatatag aagaagttat aaataatatt
    25981 aaagaggatg aaaaagagta tgtatataaa atcctttgtg acattgaagg taattatggc
    26041 attttaaatc aaataaggat aaataaatgt gtatctatag ataaatgtaa agaaatactc
    26101 aatgaagaaa aggaaataat ttatgcatat attataaaaa tagcttttga agagaagatt
    26161 gatttactag atatttttta tgaaatggaa tatgtatgga tagagaaata tctaaaatat
    26221 cttttagcat tagaaagaag ttttaattta gtactatata agtacataat tagtcgtcca
    26281 aatacatcag atatggaaaa gataagagta tataaagcta tatctaaagt agttttagaa
    26341 agtataagct taagtgatga aaaatataag gagatttttt atctttatat aatgtattct
    26401 tataagtaca taagatacat atatagcaat ttaagtgatt ttgaattaat aaaatttgtt
    26461 tgtaatgatt tagataagtt cactatagaa tttaagcatc tacttgatat taaaaataaa
    26521 gtaaataatg ctaatgaaaa attagagtat atacatagta tgaggaaatt actaaatgag
    26581 tatccttttt ataataaaat aataaaaata cttataggtg aattagaaga aaatataaaa
    26641 gaaagtgaag aatttaagga tttaaaagat agttttatta ttagtataga aaatatgatt
    26701 gaaactggca aaataaatga tgcgaaatta ttggtagatg actattcaaa aacatttgtg
    26761 gatgatataa agattttaaa tataaaggga attttataca tgtttgaaaa taagcttgat
    26821 aaagctgatt ttatgttaaa aaaggcatta tcattagatg tagaaaatga agatactata
    26881 tataatatag aatacttaaa aagtttaaga taagtcaaaa ataggagaat tgaagaatga
    26941 atataccaat agtggttaat gaaaaatcta aagacataat aaaagattgg caatatacat
    27001 gtacaaatga agatataaaa tttacaagca taataatatt gacatataat caaatagaat
    27061 atactaaatt atgtattgaa agtattagga aatttacccc aaagaataaa tatgaaataa
    27121 taataattga taataactct tctgatggaa cagttgagtg gttaaaggga caatctgata
    27181 taaaatctgt gtataataac aaaaacttag gatttccaaa aggatgcaat caaggtattg
    27241 agttagctac tggtgaaaac atattgttgt taaataatga tgtaatagtt actccaaatt
    27301 ggctctataa tttgaataaa gctttatggt ctcaagataa tataggtgct gtaggaccta
    27361 ttacgaatgc atgttcatat tatcagcaaa ttgatgttga ttataagtca attgaggaca
    27421 tgtttatatt tgcacacaaa ataaataaat ctaatattaa cctatggaaa tatagattga
    27481 agttagttgg attttgcatg ttaataaaaa aaactgttct ggataatgta ggggttttag
    27541 atgaattatt cacacctgga aattttgaag atgatgattt atcttttagg atgatagagt
    27601 caggatataa gttattatta tgccaggata cttttataca ccattttgga agtgtgtctt
    27661 ttaaagaaaa tcgtaataaa tatacagata taatgtatat taatagaaga aaatttgaaa
    27721 ctaagtgggg atttaatcca gaatatagtg gaatgataag aacagatttg atttctaaaa
    27781 tagatgatag tttggaggca aatattaatg ttttagaaat aggatgcggt ataggggcta
    27841 ctcttttaga aataaaaaat atatataaaa atgcatcaat atatggaata gaaattagtg
    27901 agtcagcagg taaacttgca aagtatagtg cagatgtact tatagcaaat atagaaaaag
    27961 taaaattgga ttataaaaaa gatttttttg attatataat attgggagat gtacttgaac
    28021 atttgattga cccatggaag atagtcaatg aattgaaaca atatctaaag aaggatggtt
    28081 atctggtatc tagtatacca aatattatgc atgtttctgt tttaagaaat cttgtgttag
    28141 gtaattttac atatgaagat tcaggaattt tggacaagac tcatttaagg ttttttacat
    28201 taaatgagat aacaaaaatg tttaatgata gtaattacat aattaatagt attacagcta
    28261 ctagtatgcc actttcggaa attgataata gcttcattga tacaatatgt aaggaaagct
    28321 gtaaagaaat gagacaacag tacgaatctt atcaatatat agtaaaagcc tctaaaatcc
    28381 tagatactaa aagatattct agtattagta tgattaattt aaaatacgct ttaatgagaa
    28441 ttgataatga attagatgta gatagtaatt taagttatat atttgataat tataatataa
    28501 ctgaaccatg ttttgttgat gatattgaat atctaattga tacatttgtg ataaataaag
    28561 caactctatt aaatagatta ggagttgagg catttaacag aggtctagga gattttagta
    28621 taaacatgtt tatgacagta ttagagatgg atagagatga tattgactca atatataata
    28681 taacaagtgt attatgtgaa ttagaagaat ttgatactgc ttataattta attaaaaata
    28741 gtagcgaaaa agtacagagt gaaagtgata tacaagagat actttgtttt attgagggga
    28801 agaaatatga ataatgataa gatatgtttt ataacctgtg taaatgatga aaggctttat
    28861 gaagaatgtc ttgtatatat aaatagccta gacattccta aaaactttag tatagaaaca
    28921 gtagctttaa gaggagccga ttctattgcc tctgcttata ataaagctat gagagaaaca
    28981 gattctaagt ataaggtata cttacatcaa gatactttta taattaataa aaatttcata
    29041 catgatattt tagagatatt taaaaaaaat acagatgtcg gaatgattgg tgtagctgga
    29101 gtaaaagata tgcctgtgaa tggaatttgg tgggattctg ataaaaaagc agggaaagta
    29161 tatgatagtc atacaggtaa aatggatatt tatgaattta gtaactttga agaatgtttt
    29221 aatgaagtta tggctataga tggattaatt atgattactc aatatgatgt attttggaga
    29281 gaggataagt ttgatgggtg gcatttttat gatttgtcac agagtatgga gtttagacaa
    29341 aataaataca aagttattgt accaaaacaa aataagcctt ggtgtatgca tgattctgga
    29401 gtagtaaata taaataatgg atatgatgaa tataggataa aatttgtaaa agagtacata
    29461 aatcttgatt tatatagaga aaatttttat gaatttgata agaagaaagc aagtaccata
    29521 cttaagagta ataagcatgt aatggattgt atgaaagagg agcttacaga acaattaaga
    29581 ttaaagaatt acaaggatgt tttaggtatt gtttctaaaa ttgctcattt ttctgttttt
    29641 aatcatactg gtttgtattc ttgtgaagaa tgtgagagag cattgctaga atgtgcaaat
    29701 aatttaccag aaatagaaaa tacgaaatta tttacaaaaa aagatttaaa aagaagaaaa
    29761 gtattacatg ttttaagtga aggatattca acaggaggtc atacaagact agttaaaaat
    29821 tggattgaaa ttgacaaaga ctctgttcat tcagttgtaa ctacatggca atcagataca
    29881 ctgccagact ggttattaaa agcaataaat gatagtggtg gcgaagttat atctttggat
    29941 ttaatttcag atgattattt aaaaagagct gtagctttga ggaaaatagc ttatgactgg
    30001 gctgatatag ttattttata tgttcatatg tgggatacaa tacctgttgt tgcttttgga
    30061 gttgatggga aaatacctat tttacatgtt aatcactatg atagtacttt ttggttaggg
    30121 acaagtattg cagatattgt cattgatttt agcgaagcta gtcaattaat tacattagat
    30181 aaaagaggca tagataaatc tgaaatatta ccaatgccat taaaagaaaa agaagtattt
    30241 tcaaaatctg aagttagaaa acgatataat ttagaggatg atgtgacaat aatattgact
    30301 attgctagtg aaagaaaata cagtagtgta aatgatatta attactttaa aattgctaaa
    30361 acattgattg aaaatacaga taatacaatt attattgttg taggagttga tacagagaat
    30421 ctctattgga aaactttaag taacgttaca gataatagag ttatatgttt tggaacaaca
    30481 gcaaatatag acgattttta caagatttcg gatatatata tggagtcttt catggtagga
    30541 agtcatactt caaaactaga tgcaatttta tatgatttaa ttcctgttaa gtttaaaaat
    30601 gaagtttcac caatttttac aaatatgtgg aattttatag ataagtttga ttataagaat
    30661 ataaaagagg ttatagatat gataaaaagg tacaatacta gtgaatttga cagggatagg
    30721 ataaaagaaa aacaatcgga aataaaacat tttgttatag ataaacactg tattgaaata
    30781 aaaaactata tagacaaaat atataatgaa gctaatcatc ataaagttaa gtatgattta
    30841 tataaaaatg atgaaatcga ggactatcag ctttttgtag cattatatgg ctatatggta
    30901 aaaaataaaa acatgtcata ggtggtaaaa tgaacaatat tcatatcaat aaatttgaaa
    30961 aaagctgtga aagaaactgg gattattttg tagaaaataa atctataaat ggtacatttc
    31021 tgcaaactag aaatttcctt aattatcatg aggagggaaa attcactgac cactcattaa
    31081 taatatgtaa aggtgaagaa atactagcag tcgtacctgc atgtgaaact tatgaagatg
    31141 gtgaaagaat atttttttct catttaggga gtacttttgg tggtattata ataagaagtg
    31201 gttttaatga tattaggcat gtaaatgcca ttacaaataa gttagaaata tatttacggg
    31261 aacaaaattt cgataaagta tatttaaaaa atacatcaag aatattttgt aaagaggata
    31321 cttcattact agaatatttt ttatttaaga ataattacat gttttatgat gaattaagta
    31381 tgtttttaga attaagtaag tataaagaag atatattagc caattttaaa tacagtaaaa
    31441 gaagagattt taaatattct ttgaagaata atcttacctt taaaagactg tatagtgatg
    31501 aagatatagt ttgttttttt aatttacttt ctaattgttt agatagtaaa ggagctaaag
    31561 taatacattc agtagaagaa cttttggagt ttaaaaataa tagattaaaa gatattgtag
    31621 aattttatgc agtatatatt gatgacatta tgatttgtgg gagcatggta tttaactttc
    31681 aagaaagagt ttatcatact caatatctag catcaaattc gacatataat aaatattatc
    31741 caatgatatt tttatactat aatctaataa aagatgcttt agagagaaat tttaagtatc
    31801 tttcttttgg aattagcact ctaaatagag gcaaatactt agacgagggt ttagctacat
    31861 ttaaagaagg ttttggttca agtgcaattt gtaacagaac atactataaa aatataaata
    31921 agtgagaatc atgttttata gagtttttag aatatagtaa atatctagta ctctaacatt
    31981 acataaaaat aattagttta taaagaaggt ttgtatatga aaagattaat gattttaggc
    32041 tcaattaagt attttgagaa tattgttata tcagctaaaa aagagggaat atatacgatt
    32101 gtttgtgata atagaattga tactcctgcg aaaaagattt gtgatgaagc aatagatgtt
    32161 gatgtatttg attttgataa aataaaaagt atagctatag ataaaaagat agatggaatt
    32221 ttaactggat ttacagattc tttaatgaaa ccatatgttt atattgcaaa tgaactaaat
    32281 ttaccatgtg tgatttcttg caatcaactt gaatctgtaa ctaacaaggc tattatgaaa
    32341 aaatatttta aaaataatgc aatacctaca actgattact gtattattga aagtctaaaa
    32401 gatttagaaa aaatacaagg attaaatttt ccacttgtaa taaaacctgt tgatggttat
    32461 ggttcaagag gtgtatattt tgtatcaaat atagaagaat taatagaaaa atttgaatat
    32521 tctaaggaag cctcacaaga tggacaagta ataattgagg aattttatga aagtgaggaa
    32581 atacaaggac tagcttgggt tcataatgga gagtcacatg tattttatat tggagataga
    32641 gagcttgtaa acatacatac aggaagagct ggaaaaccag atagattact ttatccatca
    32701 aagtattgtt ttcaatatga ggaggaaata aaatctatat atcaaaatat aacacaggct
    32761 tttgatataa aaaatggacc tctttatgta caaatgttag tgggagcata tggaataaag
    32821 gtatctgaag taatgccaag attacctgga ggttgtgact atatggctat ttctcaaata
    32881 acagatttag acataggaag tttatttgca aactttagtg tgaataatga agtaaatttt
    32941 gatgaaataa agaaatataa tatgcatcta tcaaaatgtg tatatgcatt accaatatac
    33001 ataagacccg ggacaataaa aaagataaat aatatagata aaatagaaaa cttagattat
    33061 gtcactcaat ttctattact agtaaaagaa ggagatacaa tagaattata tgaagatgag
    33121 agacaggatt gtggaagagt ttatggaata gcaaataata tatttgatgc aaatataaaa
    33181 aagaaatgta ttcatgaaat gatagaaata cttgatgaaa ataatgaaaa tatgatagaa
    33241 aatttttaaa taagggagta tgatagatat gaagctaata actcatgaac aaattatgtc
    33301 attaaacatt tctcagaaac aaggatatga gtggataaaa gaatctttct taacaaaaaa
    33361 agattgtcaa ttaccaccaa aaataagcat ggtagaagaa ggacatgtat tttacaatgt
    33421 aatgccatcc ctaaatcata atcatatgat tggtggaata aaaatggtta caagattgcc
    33481 agggagaaat ccaagtttaa actcagagat atttctatac aatatggaag atgctaaatt
    33541 aaaggctatt ttggatggta attatattac agcttttagg actgctgctg ttgctgcctt
    33601 ggctatcgat acatttgcta aaaaagattt taatactatt gctattatgg gtttaggtgt
    33661 aactgcttct gctataatga atatgctttc atatactctt aaagataaaa atataaatat
    33721 aaagttactt aaatataaag accaagcaga gttgtttgta aaaaagtatg aaaaaaatga
    33781 acaatttagt tttgaaatat gtgatacaca tgaagaactt attgtagatt cagatgtagt
    33841 tatttctact gtgacatata cagatgaata tttatcagaa tttaaatggt ttaaaaaagg
    33901 tgctttatta ttacctgtac atgttatggg atttggaaat gttgattatc tatttgataa
    33961 agtatattta gatgattata atcatttgaa acatctagaa aatatagata gatataaata
    34021 ttcttgtgaa ttttatgatg ttttaagtaa acaagctaaa ggaagagaaa atgatgatga
    34081 gagaataata tcttacaata taggtttgtc aatacatgat atattatttg caaataaaat
    34141 ttacgaattg tgtgaaacac taagctgtga agaaatagaa ttaaaagaac caaaagaaaa
    34201 atattggata tagtttattt cattcatatt tacaatagtt tattgtaaat atgaatatga
    34261 agtaatatat tgttagaaaa ttagcatgaa taggatagat aaatttatgg ataaatataa
    34321 tttaattagg ttcgtatcta ttggaaacga atatatagga aatttagttg cattagaatc
    34381 aaataaagta ataccttttg atataaagag agtttattac atatatgatg taccaaacaa
    34441 tgtaaagaga ggatttcatg cacacaaaaa cttggaacaa gtactagttt gcctaaatgg
    34501 aagtgtgaag ataaaatgtt atgatggtgt aaaagagact atatttaatt tacataaaaa
    34561 tgatgttgga ttatatatag gaacgggagt gtggcatgag atgtttgatt ttgatgaaaa
    34621 aactgtttta atttctatag catctgaaca ttataatgaa gaagattata ttagagacta
    34681 taaagaattt ttaaagtatt taaataatgt gtagttatta tacaattagt gatgtatttt
    34741 cttaattaaa taaatttaag atagccacat agagaataga tagattagga tgtgaattta
    34801 ttgttggtta aatttaataa tttattaaaa cagttccaaa ctcataaaga agaatatgaa
    34861 cagaagatta aggatgtaat ggaaagtgga tattatatcc ttggaaatga agtaaagcat
    34921 tttgaagaag aatttgctaa ctatgtaggt agcaaatatt gtgttggttt agccagtggt
    34981 ttagatgcac tgataattgc ttttagagca ttaggaataa gcaaaggtga tgaagtaatc
    35041 gtgcaatcaa atacatatat tgctagtgtt atgggaatca gtataaatgg agcaacacca
    35101 gtatttgtag aaccaaatga atattataat atagattcat ctaagataga agaaaagata
    35161 aatagcaaaa caaaagctat attagtagtg catttatatg gtcaagcaag taatatgaaa
    35221 gatatacaag aattatgtga taagtataac ttgtatttaa ttgaagattg tgctcaatct
    35281 catggagcta aatatgatgg aaaaatgaca ggaacatttg gtgatatagg ttgttttagt
    35341 ttttatccaa gtaaaaattt gggtgcattt ggagatgcag gagctatagt aactagtaat
    35401 gaggaaatag ctgataaagt gaaaattctt agaaactatg gctcagaaaa gcgatattat
    35461 aataaatttg tcggatataa ttcaagattg gacgaaattc aggcaggttt attaagagta
    35521 aaacttaaat atctaaatga aattgaaatt gaaaaatcaa aaatcgtaga tagatatctt
    35581 aatgaaatta ataatcccaa agtcatactt ccaaagatag aaaataattg tactcatgta
    35641 tggcatttat ttgttttaca agttgaaaaa cgagatacat ttatggaata cttaaagaaa
    35701 agtggtatag agtctataat acattatccg ataccaccac atctatctga agcatatgct
    35761 tatttgggat ataaaaaggg gagttttaat atagctgaga actattcaga taaagttgtt
    35821 agtatacctt tatttattgg tatggaagat gaagaagtta gttatataat taaaattata
    35881 aatgaatatt aaactactca taataatatc aaaaataaat tttttatata tcatttattt
    35941 aaatttcgtt aattgacatt tttaaatatt gatatattgt acaaataaaa ttgaa
//
LOCUS       DAMJZE010000020        35109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJZE010000020 DAMJZE010000000
VERSION     DAMJZE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.020"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            31..213
                     /locus_tag="PC541_000908"
     CDS             31..213
                     /locus_tag="PC541_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838381.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIEHSGVVEGTQPTK"
     gene            276..707
                     /locus_tag="PC541_000909"
     CDS             276..707
                     /locus_tag="PC541_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2838382.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            707..1546
                     /locus_tag="PC541_000910"
     CDS             707..1546
                     /locus_tag="PC541_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2838383.1"
                     /translation="MKICITVGHSILKSGACTSANGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKSEEKSYKIPKVNSGGYDLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGVKLDKSLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIIYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKIGSITKEKYTMIKGNDRFDTLYKALDF
                     IDR"
     gene            complement(1597..1809)
                     /locus_tag="PC541_000911"
     CDS             complement(1597..1809)
                     /locus_tag="PC541_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838384.1"
                     /translation="MTWKNEIKSILVKENISMNELNNLMNEHNNKNNTVENLRQKINN
                     ETMKYSEILTIANLIGYEVVWKKKEK"
     gene            1988..2818
                     /locus_tag="PC541_000912"
     CDS             1988..2818
                     /locus_tag="PC541_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838385.1"
                     /translation="MKTLFKEAHKIAREIKEKYNDVDYKVQFSLCLSFLNKKGDIKMK
                     ELKGSEKQIIWANDIRADILNLTNELEKSKLERIKNEDYKIKDMSIEEMIERCKRKFE
                     RIREAISNIEDAKFFIDNFRNVLKYNSLNQKAFQINQIFRESQFTEEIGNLKFLKGET
                     QASHKLRKVEGSISYEEAKKIAKEINFNDNWSNEIKENVLEIVEIFDDAVKQATSTEY
                     IEKAKKYIIETLIQKNAQFYRNGFADIKKKQIENYRKIESAERKIEIAYQIFRDGVEC
                     "
     gene            2897..3400
                     /locus_tag="PC541_000913"
     CDS             2897..3400
                     /locus_tag="PC541_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838386.1"
                     /translation="MNIDFSTILNLKVNKENVEMIEKEINKINNDIFDISLKDCANGH
                     VGIVKETVGKSLNIETWGHGEINSSIGIRLRFNFYYSKGDKVDIENIENILIYVNSLC
                     SKNVESDLIASVRPGFYKLEEKYSTRIEKCEIRHEFTGINKQEIKEFLEYLTEIEKSE
                     RTVIYKK"
     gene            3424..3894
                     /locus_tag="PC541_000914"
     CDS             3424..3894
                     /locus_tag="PC541_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838387.1"
                     /translation="MNKQKARRFLRVIDMNMDKIEEEAIKAFKESCLIKETNNIKIYI
                     DIQGKVEAIAVQTWAKLLDDDKEINIFTLNQTPTHLNDLFGEICYVNDYEEFENWCEN
                     EREILDWDSYKKFNKENFEEIAERNIDDSTSVFLEELQKSIESCKQELQNIVEN"
     gene            complement(4192..4473)
                     /locus_tag="PC541_000915"
     CDS             complement(4192..4473)
                     /locus_tag="PC541_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838388.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVTNYLRFAEHDNDMKIKL
                     FQTIIEAVARKRVTLPADNEFTQGIMLSNELEMTTNVGFIIQGLSYHNN"
     gene            complement(4617..4949)
                     /locus_tag="PC541_000916"
     CDS             complement(4617..4949)
                     /locus_tag="PC541_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838389.1"
                     /translation="MPSFNRDLLVDNEKPILKTGTIVQNDSTTTDNKTLNIYNLVNKK
                     SKKVTMSATLDEELVNKLKSFSVDMNSDVSKLLSDILTKILSDVTIKEENLNIYNERN
                     RRNKTKKK"
     gene            complement(4950..5759)
                     /locus_tag="PC541_000917"
     CDS             complement(4950..5759)
                     /locus_tag="PC541_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2838390.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNKNYTNVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIILIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTVLVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFTNMLEELKRKGVL"
     gene            complement(6046..6228)
                     /locus_tag="PC541_000918"
     CDS             complement(6046..6228)
                     /locus_tag="PC541_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2838391.1"
                     /translation="MKKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
     gene            complement(7049..7201)
                     /locus_tag="PC541_000919"
     CDS             complement(7049..7201)
                     /locus_tag="PC541_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838392.1"
                     /translation="MDNFLLNILAGVIASLIFYIISKLFRQVKNHSAQKSGWEFDLKI
                     KFKKSR"
     gene            7694..8401
                     /locus_tag="PC541_000920"
     CDS             7694..8401
                     /locus_tag="PC541_000920"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2838393.1"
                     /translation="MSLEEAKELEKNYKLPMPTFCLKEKGYFAREYAALMLLSNGGMK
                     IMHTEKNRYLYQNKFDIDALAKQLSISRATLYRNIKKLEELKCGILKIENTKNGTVYR
                     LKYGIETGYNDEVHKFVTIHHKILEELVINFKTPSVKLYCLLCYLTNEIDFKVIDEEF
                     LCNNIGLCGKSESNKNKVRSMIRVLMKCKYIDVKKENRTFWNEETKNKINKTLKLYRL
                     CSYEEWKNLNDKTDFKD"
     gene            8946..9620
                     /locus_tag="PC541_000921"
     CDS             8946..9620
                     /locus_tag="PC541_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838394.1"
                     /translation="MMLKYITKHKNMEESRMKKVLLLITVCLLSVGLVACSSTNNKDT
                     GNTTKKEKKANENEVDGIKITVKDVTKEDIKGDINEDGSFNENGEYFSDGIEKKKAAD
                     YVYEVVNVEVENKTDKAVKLFQTGWNAVGTDGYEFKDIKVTDKLDNQQVPANYKFDAQ
                     VKILVEKNMNVKEIVLKYNLKDYSRLFEAMEYANQGASKSDVEKKFPELYKDNWIELG
                     EIKVEQ"
     gene            complement(9650..9949)
                     /locus_tag="PC541_000922"
     CDS             complement(9650..9949)
                     /locus_tag="PC541_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838395.1"
                     /translation="MESLLKYHRKKKKLTQRRLSELTGISQSYISRMENKKFKHSPTL
                     TQIILLADILEIDQLILSEFFLEKEREHLKNTKKYNKKAIKTLSRDFLKEYKNKD"
     gene            complement(10132..10476)
                     /locus_tag="PC541_000923"
     CDS             complement(10132..10476)
                     /locus_tag="PC541_000923"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838396.1"
                     /translation="MNIEERLKQLILSKYKSIRAFTKEVNIPYSTVDTMLKRGISGTS
                     ITTVLKICNALNIDADALIDNELKEKELSSKIQTNQFEQKILKMYNALDEHGKDIVFT
                     ILEKEYVRINKL"
     gene            10705..10869
                     /locus_tag="PC541_000924"
                     /pseudo
     CDS             10705..10869
                     /locus_tag="PC541_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            10906..11121
                     /locus_tag="PC541_000925"
     CDS             10906..11121
                     /locus_tag="PC541_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838397.1"
                     /translation="MKLLKSKNIKIGEIIRLHDVKFNHDRKCIVTYIDKDEIVLTYYD
                     EEIKEMTCKVLTKEDLIFNDYELKLLS"
     gene            11542..12027
                     /locus_tag="PC541_000926"
     CDS             11542..12027
                     /locus_tag="PC541_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2838398.1"
                     /translation="MPSNKTNINKTNLTNNIYSSAEQKEEKTKDKEIYKRIVDYLNKT
                     ADKSFKSTTKKTISLIDARLKEGFTEEEFYKVIDNKVLNWLDNDKMNAYLRPETLFGN
                     KFESYLNETPKKSLKGNESVKGKTKTNNNKFANFNQTFDKYSEDELDAIIKKSQSAKF
                     K"
     gene            12052..12654
                     /locus_tag="PC541_000927"
     CDS             12052..12654
                     /locus_tag="PC541_000927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838399.1"
                     /translation="MEINSVRELRELFELFTVIKAEGNNVVSCNETVNELSNKELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKKIENQEDVFKIPF
                     LTRDYLSSAKEIKNIEICEKCLDGNSWSIIRRKQFKKMKK"
     gene            12700..12981
                     /locus_tag="PC541_000928"
     CDS             12700..12981
                     /locus_tag="PC541_000928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838400.1"
                     /translation="MIKNNVLSYEEKIKVLKKHGYSDFTKEYVLKEFDKEHAFYKSFL
                     RAIEKEDDGKGVLIKKIGKGQHQFSKGFIKTHTISELLEEAKRIRKILE"
     gene            complement(13020..13364)
                     /locus_tag="PC541_000929"
     CDS             complement(13020..13364)
                     /locus_tag="PC541_000929"
                     /inference="COORDINATES: protein motif:HMM:NF022172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HDF2838401.1"
                     /translation="MVTNQDCIRDILLLIESNTTPISPVLQFDTILDNLTYDKSTIVH
                     HINLIYQNNLVDDVNYGDDTVLEVMQLSLKGYQYLDKIRDNTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            13453..13629
                     /locus_tag="PC541_000930"
     CDS             13453..13629
                     /locus_tag="PC541_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838402.1"
                     /translation="MQKRITEMSILEKVDLIGQKQKKLQEKYKNLSKGELFSIACNEV
                     KKIEEQEGKYFEYR"
     gene            13655..13981
                     /locus_tag="PC541_000931"
     CDS             13655..13981
                     /locus_tag="PC541_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838403.1"
                     /translation="MEELKEEIKQKEENLKEREKYIKELKERLECFARDALESEECII
                     DIENILIEQTVELEAYNLFIDCILNKDIRKAIAIYNRTKSIRIKHKNINSISKKIEKI
                     KQIISL"
     gene            14001..14174
                     /locus_tag="PC541_000932"
     CDS             14001..14174
                     /locus_tag="PC541_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838404.1"
                     /translation="MDKFVTCARCERTINVEENNHVKYEEETLNLSFTLYFCLGCVDK
                     LIEQSKMLEGENE"
     gene            14167..14382
                     /locus_tag="PC541_000933"
     CDS             14167..14382
                     /locus_tag="PC541_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838405.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYTLEYRSKFYEWIKLLE
                     RKGSLQNYKIVEVLNAERNGEEASFWN"
     gene            14441..14614
                     /locus_tag="PC541_000934"
     CDS             14441..14614
                     /locus_tag="PC541_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HDF2838406.1"
                     /translation="MKMINVFKLSSMYCICPKCGSDKLGEGEGKLIVDEHTYYRECKC
                     GFNVLVDEREDEI"
     gene            14629..15036
                     /locus_tag="PC541_000935"
     CDS             14629..15036
                     /locus_tag="PC541_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2838407.1"
                     /translation="MNSVILIGRLTKDPELKYIPGSGTAVSTFTIAVDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLGKGRLVAVEGSIKVNSYEKDGEKRTYTKVHANKIKFL
                     DYKKDDTEKEYMFEPKGLDQQGFQAIDDPNIPF"
     gene            15053..15202
                     /locus_tag="PC541_000936"
     CDS             15053..15202
                     /locus_tag="PC541_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838408.1"
                     /translation="MSNTNKKTAKICGWCGTTVYSYMDGKIAYCSKKCKKKREKNLET
                     EGAKK"
     gene            15199..15447
                     /locus_tag="PC541_000937"
     CDS             15199..15447
                     /locus_tag="PC541_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838409.1"
                     /translation="MKEYRIEIGEMIFLKHKVIVEVPEQICAFDIYKYIENECQDKSE
                     ISEYIKSIGGRQLNFIEGDSGDSELIVGSCKKIKKGEK"
     gene            15449..15739
                     /locus_tag="PC541_000938"
     CDS             15449..15739
                     /locus_tag="PC541_000938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838410.1"
                     /translation="MKKTKIEEFITRVKESMQSVAKESIKDLENILKEIETGETEVKI
                     LFIDKGKKVEEIVEFTHDEYVKMIIEKMIFDSLKIIKEQHEKILKEFEEREV"
     gene            15742..16002
                     /locus_tag="PC541_000939"
     CDS             15742..16002
                     /locus_tag="PC541_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838411.1"
                     /translation="MNFKMIANIPKWTVKEQFEKVLEEVLELKEAIELKDNKKIMEEG
                     LDVIQAIFTLFKVLKIDKEIREGLKLHNKKLRKRKWKLEKIN"
     gene            16020..16229
                     /locus_tag="PC541_000940"
     CDS             16020..16229
                     /locus_tag="PC541_000940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838412.1"
                     /translation="MSICRKCLYGDYLDELVVGECILCGQEFFSIKTPVGKMCPSCSE
                     MFKGNCSQCAKPIGNEKKSFKNKLK"
     gene            16472..16933
                     /locus_tag="PC541_000941"
     CDS             16472..16933
                     /locus_tag="PC541_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838413.1"
                     /translation="MKIKKEYFDKVENALKMLGDKYRKLRVLKKEIAILKHKENYREI
                     NYEELGFKVQKSVKGIDDMVVTIEDAIYNKETEIEIIERKLEFYNIYLKELSEIEQKI
                     IELVYFYSWNGKMPITKMAMELNYDRTSLYNKKAIAINKIALMIYGDEALE"
     gene            17113..17661
                     /locus_tag="PC541_000942"
     CDS             17113..17661
                     /locus_tag="PC541_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2838414.1"
                     /translation="MNFVEPIRNLEKLESICAYLEETNMRNNVLFSMGIYTGLRVGDI
                     LKLRIKDVHDKRFITLKESKTGKTKPIEVNPILRRILKEYTKDKNQNEFLIKSREQTN
                     KAISRVMAYKIIREVGEMFGIDNLGTHTMRKTFGYHYYKRTHDVVTLQKLFNHSSATI
                     TLRYIGIEQDDLNKAYRNIKYF"
     gene            17834..18106
                     /locus_tag="PC541_000943"
     CDS             17834..18106
                     /locus_tag="PC541_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838415.1"
                     /translation="MNKYGKFKCNNCGKKVNVNNRDLKTKVIDNTTVTYFMCRRCFEK
                     FVVSCEDDYIKHKREEYKKLKDDEVKLLQDMKEYSDGLGELYINEL"
     gene            18096..18428
                     /locus_tag="PC541_000944"
     CDS             18096..18428
                     /locus_tag="PC541_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF2838416.1"
                     /translation="MSYKHMTLDRLKELILIDRLDIFYRSTDWRKLRKKALERDNNEC
                     QICKEKKKVSKAECVHHIKEVKEYPGEALKLNNLTSLCNLCHNEIHEKILKINQEKKK
                     KFFSEERW"
     gene            18600..19106
                     /locus_tag="PC541_000945"
     CDS             18600..19106
                     /locus_tag="PC541_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDF2838417.1"
                     /translation="MDDNEELEIRRDVQNDYVLGMTYKEMSAKYNIPENTLKSWRKRG
                     GWTRNCTPKKGCVKNGLQESGNNLKNSIRKDLLRQIKENNMTEEYFKDLVEDYLKMWD
                     IKNELIEDVKTRGVQVKYKNGQNQWGYKKNDSVSELIKYNAQMLSLLDKLGIKATPKV
                     LGDDDIEL"
     gene            19090..20718
                     /locus_tag="PC541_000946"
     CDS             19090..20718
                     /locus_tag="PC541_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDF2838418.1"
                     /translation="MILNYKYNKYIDEYFNLVDKGEIVLCKEMELAVKKIKKDLKKPN
                     IYIDHEKVEKAIEYMEKYFYKMYAWEKFVVALVVGCFEGDELLYDEIFIMMGRGNGKN
                     GFISQLANYLQTHLHGVKNYGIDIVAMSEEQAKTSFDDVYNMLSEHPAICKKFFNRTK
                     EVITFKKTNSYLKFRTNNAKSKDGLRPGCIIFDEVHAYENYDNIKVFTSALGKVKNPR
                     VFYITTDGNVRGGVLDDFKEESKQILNEEIKNSKMLPLIFKLDSIEEMENIDLLEKAN
                     PSIKYNSDLRTQILKDIDKAKNRPQMMIELLTKRFNLPAQNLATVVAEWDDIVATNEE
                     LPDLKGFSCIGGLDFSSIRDFTSVVLLFKKDKKRYIMHHTFICYKSLEITNFKFDIEL
                     AKQKGLCTIIYDETIKAEYIADWFIEKAEDYNILKICCDSYRAAHVKEEFANKGLPIE
                     EVRNGSITHAKIAPLVEQIFAERTIKWGDDMMMRWYTNNVYVDTDKKGNKTFLKIEPI
                     KRKTDGFFAFLHALSKDGELMQESTIKFYDCYTY"
     gene            20782..21927
                     /locus_tag="PC541_000947"
     CDS             20782..21927
                     /locus_tag="PC541_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2838419.1"
                     /translation="MSGADGEYFKKLEATIFYKEFALRSCISIIANALVLSEFQTFIN
                     SKSVKEKNYYLFNIEPNQNQNATEFWQEVITRLVYDNECLVIQENEQLFVAESFNVDK
                     YVFYEDVYKNITIRNYKLDRSFRESDVLYFKLNVESIKTVIDYLYNDYKDLLSSAMKG
                     YKKNNSEKGVLEIDTNYPQTKEAQERLNDLMSNKFKRYFESDNAVLPLSNGLKYTENT
                     KASSIKDSRDIRAIIDDVMDFVCAAFHIPAGLIKGNVSGVEGITDNFLNFSVNPIARL
                     ITTEITRKMYGRDKFFEKSYVKVDTQKIANMGLEKISKASDLLFRIGVNSINDNLEML
                     GREKINESWADEHYVTKNYQSILNPDLKGGANNGNGEKSGIAGNKGQ"
     gene            21887..22516
                     /locus_tag="PC541_000948"
     CDS             21887..22516
                     /locus_tag="PC541_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDF2838420.1"
                     /translation="MEKRAVSLEIRDSNLESRKISGYAAIFNDDYTKLQDRWGDTFYE
                     KISRGAFLKTLADNTRDKFMLINHDWNKVIGRTNSNLILEEDERGLRFELDIPATTDG
                     NDLLENVRLGLIKGCSFGFNIVNSKTRFDDDWTYYRDITEVELFEITATPIPAYNDTE
                     INCRSDISIKELRESQKEKVKQNDNKDNIEKRNVDLVSAFFNAFNRGGK"
     gene            22520..23686
                     /locus_tag="PC541_000949"
     CDS             22520..23686
                     /locus_tag="PC541_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDF2838421.1"
                     /translation="MAIKNLDEATNVELRTNLMEAIKSNDEEKITTSFLRMAEDIQSN
                     LLKEARSIANIESADRAILDKRGMPQLTSEERAYYSQVIEKRGFTDLEVTMPRTIFDR
                     VFDDLEQNHPLLSAITFENTTGVTEWVIRKTGTEAAWWGQLTDPIKKELEGGFAKINT
                     VAYKLSSYLPVAKSMLDLGAEWLDRYVRVVLTESISLGLEMGIVAGTGKDQPIGMMKD
                     LKGSVVEGVYPDKTAKPLADFKPTTLGTEIMAPLTNDGKRTVASVIMLVNPVDYWAKI
                     FGATTMLTSNGTYVYGVLPIPGNIIQTVAVPQNKLIVGVAKDYFMGIGSAGKIEYSDE
                     YKFLEDVRVYLAKQYATGTPKDNFSFLVFDIENLDTTLATEINSNTKTSAARTK"
     gene            23696..23983
                     /locus_tag="PC541_000950"
     CDS             23696..23983
                     /locus_tag="PC541_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838422.1"
                     /translation="MENENIKSIVEEVKEYLNVTWIEEDAKIEKMVVRGIDYFENEIA
                     GIALDFKDNFNRELLFNYCRYVRNNAIEYFEENFSKELLRLQIKSAANEVI"
     gene            23986..24636
                     /locus_tag="PC541_000951"
     CDS             23986..24636
                     /locus_tag="PC541_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDF2838423.1"
                     /translation="MKKIKFNNFNDGIVEFGEYKESYDIEGNALDEKEFIVSGKLFYS
                     NEYIREQDKLKFDNTGLKVSIKLKVPYMRLIKTSDVIKLNNEFYSIVKLDISNDKTSI
                     YLYLSELVDVLDKHISIFIKERKNVLEDVAMKFYKKIWACVEDLNSKELIENNSIKTS
                     TNKKFKIRYIEELDLSIKQNAITNFVIKYKDKFYNITQILNLEEENKILEITAESK"
     gene            24656..24982
                     /locus_tag="PC541_000952"
     CDS             24656..24982
                     /locus_tag="PC541_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838424.1"
                     /translation="MHSKIVTILKKLNLGIGFQEYDNFSNDEEYIIFFIENEYDANFS
                     DNENKSIVYSIKIEYWYKSLKNINKYISIKDIFKKENFIFLNSSDFIENEYYCKELKF
                     KYEEVV"
     gene            24983..25426
                     /locus_tag="PC541_000953"
     CDS             24983..25426
                     /locus_tag="PC541_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2838425.1"
                     /translation="MGVEITTEGFDAILSKIESMGKSGDKLLNEAVKAGGNVILQDAL
                     PRVSKRSGKLKDGLKVSGVKKKGGTKYVLVGITKEDNSKIFYGKFLEFGASAHQIPIK
                     KGKKKGRVINHPGVSPKPFLAPAYESKKDEAKNVMKEILKRGLGL"
     gene            25426..25761
                     /locus_tag="PC541_000954"
     CDS             25426..25761
                     /locus_tag="PC541_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838426.1"
                     /translation="MININNLVIETLKPLKIPVSFQRYNGEEKTYITFFSYLEQNFYA
                     NDEVVGTEHYIQIDLFSKNKMSYINKEIERLLKKNNFIKRSIHEIKESDYSYHTVFRF
                     LFFAKNEED"
     gene            25765..26361
                     /locus_tag="PC541_000955"
     CDS             25765..26361
                     /locus_tag="PC541_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2838427.1"
                     /translation="MAIIGLRNYRIVKLLTDTEETLEYDTKITRLTGAKSVKISPKVD
                     SAENYGDDQLLETASAMGAIEVEIEVADLTLEERALILGYQYKDGVLIEDKNFNPPNL
                     AFGFESPKSQNGSRMVWLTKGVCEPFEEEAKTKEDKIEFQSQKIKLKFMPRINDGRHK
                     ITADTDVENAPTDVEFFTTEFLKTGAKPTAPAKANLNK"
     gene            26379..26666
                     /locus_tag="PC541_000956"
     CDS             26379..26666
                     /locus_tag="PC541_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838428.1"
                     /translation="MEIKLTINEEEKIFKAPPIALKKLDNAFALSDKINDGLSMRGLF
                     QELLDFTIDIYGEQFTKEELLNGFYPADEFMSKALEDLSKVSGTFEEKVKN"
     gene            26750..26905
                     /locus_tag="PC541_000957"
     CDS             26750..26905
                     /locus_tag="PC541_000957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838429.1"
                     /translation="MFLFEKNWSMVDIDNMDIFYYLDILAYMKKNKSDKNNPNENDVY
                     IDQLSWL"
     gene            26918..27133
                     /locus_tag="PC541_000958"
     CDS             26918..27133
                     /locus_tag="PC541_000958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838430.1"
                     /translation="MCKYCEVEESEGAFKRGKTDRGELITCNCDIEMWIEKEGNEYFL
                     NSECWCGYYSESFEPINFCPFCGRTLI"
     gene            27196..32397
                     /locus_tag="PC541_000959"
     CDS             27196..32397
                     /locus_tag="PC541_000959"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2838431.1"
                     /translation="MAEDVGELVVRVAMENSSFQQGINNLNRQMKLIRSEFKNSGAGV
                     KNFGTSLDGLKSKQQMLSSTIQQQTKVVEAYKNKLNESKTTLSNTAQKQVELKEKVNQ
                     AKSAYEQSKATLGENAEETKRLKSELDSLEQEYAKNEEKIRSNSAAVDNWSIRVNNAE
                     SRLSEMRSELDDTTERINQQENKWNQLATKMTEIGNKFSSVGKQMQDVGKKMAMSITA
                     PVLAVGAAASKLGMDFEASMSNVQGLSGATADEMVQLEKAAREAGASTSKTAKDAADA
                     LGYMALAGWDAKTSMEALMPVLRLSEAGNLDLARTSDLVTDSMSSLGLSVKELPTYLD
                     QVAKTAASSNTNIDALMEAMIVSGGTFKNLNVPLSEANALLGILANRGLKGSEAGNSL
                     NSIMINLTSGAGQAGVAMKELGLSAFDSNGKFKGMANVLMELKEKTKDMTEEQRTMYL
                     SMIGGKTQISTLQALLSGVGEEYSELEKKVGNSTGALDKMAKTMQNNNKGSITALMSA
                     VEELGLKIYDVLKPSIAQAVSWFQNLTNKLNEMKPSTVETIVKIAGLAAALGPVLLIS
                     GKIVSMIGGVIGAFGTFSAAMAVVTTGAAAATPAVGALAAILGALSSPAAIAVAGITA
                     LGISVATVAKDMSKDAIEPISRFGKSISESTKEAVSAFMDLEEKTTVSLNQLMWSGET
                     VSEKMKQTIVSNFTEMSNQIVAKLQESKEQGIRSLQEMFATSKNLSDKEKEELIKNTE
                     EAYSSKEEKIKKSNEKVNQIMTKASQENRALTKEEATEINQIKTDMLNTAVDTMSKSE
                     AEQAAIMERMKANHVDLSAKEAAEVVKNSIKKKDESIKAANEEYNERLKIAAQLRAEG
                     GAENEKLADKVVEEATRQKDESINKAKEMHQGVVAEAQKQAENQASKVDWTTGEIKSK
                     WQVLNDTVKGKMQEWDDMAGNWVEGLKEKISTGWANLKENTAILWDNIKLTVTTKWTE
                     IKESIAFKIEEIKQSISEKWESIKLFFTTTWENIKMIFGNAWDAIKQQVEEKFAPFVE
                     RLKIIFDGIKEFFRSTWEVIKNIVLGAVLLILDLVTGNFDKLKTDAIQIWENIKQSLS
                     DAWESIKEVFNNALTAISDLLSNMWEGMKSTASSVWESITQTLSSIWENIKSTCSNAW
                     EGLKTICSNTWTNIKTTLVTSWENMKTAVSTKLTEIKNNIVTVWNSVITFFKTLPAKL
                     KQAAIDMFTRLKEGIDSKKQDAVNASQKVGTDVVNKIKEFPSKFLQVGKDFVSGLANG
                     ISGFAYKVVDEAKKLGQKAADAVRKVLGIHSPSRVMAEIGKFVDEGLAQGIANNSDSV
                     KKATEKITKIIEEETKKATKACEEDIKLFNKQIDELSKQEKAALKKVKGDAKDALKEE
                     YAAKKEKIKEAIALRKEQKEKEVEQLKEIANSAKEQLNKELQDRQEFVKKVDDLTKAI
                     VDALKEKYKQEYEAQQASIKKELDALDKWKEESIDRINSVYDAKIKAIDNELEAFEKA
                     EKEKDRLEQDKEELNKIEEIKTAIKFEHDDTNKEQLQKELEKVLAERKKRIEQQEIED
                     KKEALKKRKEELEEKKKDEIENINQIYESEKEYYNKRLEDAKKFYDERIKESKLQAEA
                     EKLIMDKNQKEIVALLNSYSDAYKNAGQTLGEKLLEGFKPAINEIKNLIDSITREINN
                     ARNEALSLNSDIRKGNSAANSNSGRREATYNNNSRTANYNVNVNSTDSNTAREIERTI
                     RSLAFSL"
     gene            32429..33295
                     /locus_tag="PC541_000960"
     CDS             32429..33295
                     /locus_tag="PC541_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDF2838432.1"
                     /translation="MQKLICRNSKGQEVKLGNSRPFLLEKIENGANTSANISVSNNLG
                     DGVSIDDISIKERLLPISAAIYAKNKEDLELKRAYLTSLFNPKLGEMEFIYTNNALTK
                     RVKGVVQDITFQTPVGLMQKFLIQLLTPSPFWMDEFVLKEEVALWVGDFEFTLEISNE
                     GIEMGHRVSNLICNVVNNGDVECGMKIQFKALATVDNPSLFNVNTREYIKINRTLEAG
                     DLLEITTEFANKRIELVKSNYERINVFNWIDLDSEFLQLEVGDNLFRYDADVGIDNLE
                     MSIYYNPLYLGV"
     gene            33297..34376
                     /locus_tag="PC541_000961"
     CDS             33297..34376
                     /locus_tag="PC541_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus ReqiPepy6 Gp37-like family protein"
                     /protein_id="HDF2838433.1"
                     /translation="MQQSIRILDKNINLIAEIDNYEELQIIKKFHKVGEFSLKINANK
                     NHVDKLVKNNIILLGKNYNKVCLILHREFMYTETGEKSDILSVKGVDLKGLLNRRLII
                     PDTGEAFESHEGTQEEIIKAFVNNNCVDPSNPKRVIDNLIISENKNLGSADMWRSSYE
                     NLSDKIQEISEFCGLGWEVVLDANKKKFIFDVITGKDLTINQTENPPVIFRSDFNNIR
                     ARHFTESIINSKNVVYAGAREDATKLVISTGEVEGFERNEVFADVSENTISILQKEGE
                     IKLKEYEELKSFELEIDPKNTFMYEKDYKLGDIVTVQDRKLKVTMDTRIVEVQESYSK
                     NGMKLKITFGSSIPTLFSKIKRMVK"
     gene            34376..>35109
                     /locus_tag="PC541_000962"
     CDS             34376..>35109
                     /locus_tag="PC541_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838434.1"
                     /translation="MEKSFVFNSVNGDRRYKAEDFREYFASFIGNGVFPNPSSNLQVV
                     DNNNNNMTITVKKGKGWINGAIYINTDDLVINIDPADGILNRIDRVVLRFDTLNRNIK
                     LAVKKSAFNSSPAATELQRDADAYELGLADIYIRAGAISITQSSITDLRLDKNLCGIV
                     KGTIEEIDTTTLLAQLSTWKDEEISNFNDWRENQENKQEDWYNTTTTNFVSEFNIWFE
                     SIKDILDEDAAGNLLNEINKVKEELA"
BASE COUNT        14548 a         3850 c         6239 g        10472 t
ORIGIN      
        1 tgaaaatgaa aaggtaggag gaattttata atgaatataa ataatgttgt agtaagaata
       61 ttagcagaga gaatattaaa cggaggctta aaccctttaa aaaatcgaga atttgaactg
      121 gatgatgtaa ctaatgcaga atacagaaaa gcagtagagg attatattat agagcatagt
      181 ggagtagtag aaggaacaca acctacaaaa taggttcttt ttttataggc ttagataatt
      241 ctaagtctta ttttaatgca aaataaggag gaaatatgaa cgtaacaata gtttttttag
      301 caacaaatat atttataaaa ttagtaatat tagcaatagc atttgataca ttgctaggtt
      361 gtttaagagc aataaaaaca catcagttta attcaagttt tggaataaat ggaggaataa
      421 gaaaagttgc aatgatagca tgtatatttt ttctagcagt agttgacatt cttacaaagt
      481 ttaacttttt atttatgtta ccacaagatt gggttgattt tttgcgatta aatcatcttg
      541 gaatatctga atttttctct attttattta ttctatatga aagtgtaagt atattaaaaa
      601 acatgtattt gtgtggatta ccagtgccta aacgattaaa agaaaaaatc ggtagtttat
      661 tagatactat gacagatgaa ttaaatgtta aaggaggtag taaataatga aaatatgtat
      721 aacagtagga cacagcattt taaaaagcgg ggcatgtact tctgctaatg gagtagttaa
      781 cgagtatcaa tacaataaat ctcttgcacc agtattagca gatactttta gaaaagaagg
      841 tcataaggca gatgtaataa tatgccccga aaagcagttt aaaactaaga gtgaggaaaa
      901 atcttacaaa atacctaaag ttaatagtgg aggatatgac ttacttatag agttgcattt
      961 aaattcaagt ggtgtaggag cctttggaac agaagtattt tactatagtg aaaaagggaa
     1021 ggaatatgcg caaagggtag tagataaatt atctaaacct ttcataagaa aaaaaggaga
     1081 taaagaagta ggtaatagag gtgttaaatt agataaaagt ttatatattt taaatagttc
     1141 taaacctact gcaatactaa ttgaaagttt cttttgcgat aacaaagaag attatgagaa
     1201 agctaagaaa ctaggtcatg aaggtattgc taagttgatt gtagaaggtg tattaaataa
     1261 aaatataaat aatgagggag ttaaacagat gtacaaacat acaatcattt atgatggaga
     1321 agttgacaaa atccctgcaa ctgtagttgg ttggggctat aatgatggaa aaatactgat
     1381 atgtgatata aaagattatg taccaggtca gacacaaaat ctttatgttg ttgggggtgg
     1441 agcatgtgaa aagataggtt ctattactaa agaaaaatac acaatgataa aaggaaatga
     1501 tagatttgat acactttata aagcattaga ttttattgat agatagatta agaaggtagc
     1561 aactaatttg ctagttgcta ccctcttttt ttatgcctac ttttctttct ttttccaaac
     1621 aacttcgtac cctattagat tagcaatagt caagatttcg ctatatttca tagtctcatt
     1681 atttattttt tgcctcaaat tttcaactgt attattctta ttattatgtt cattcattaa
     1741 attatttaac tcattcatgc tgatattttc tttcacaaga atacttttga tttcattttt
     1801 ccatgtcatt ttttcacacc tctcttattt atataataac ataaaaaaat taaaagtaaa
     1861 agaataatct atgttaatca aagttatatt ttcggtaatc aaagaaaaaa ctttgaaaaa
     1921 gtgttgactt agatattcat atagattata atataattaa caaataaaac aagggggtaa
     1981 gcaaagaatg aaaactttat ttaaagaagc gcataaaata gcaagagaaa ttaaagaaaa
     2041 atataatgat gtagattata aagtacaatt tagtctttgc ttatccttct taaataaaaa
     2101 gggggatata aaaatgaaag aattaaaagg gtcagaaaaa caaattattt gggctaatga
     2161 tataagagca gacattttaa atttaactaa cgaactagaa aaaagtaaac tagaaagaat
     2221 aaaaaatgaa gactacaaaa tcaaagacat gagtatagaa gaaatgatag aaagatgtaa
     2281 aagaaaattt gaaagaataa gagaggcaat aagcaacata gaggatgcta agttctttat
     2341 agataatttt agaaatgttt taaaatataa ttctttaaat caaaaagcgt ttcaaataaa
     2401 ccaaattttt cgagagtctc aatttacaga agaaattgga aatttaaaat ttttaaaagg
     2461 agaaactcaa gcgtctcata aacttagaaa agttgaaggt agtataagct atgaagaagc
     2521 taaaaaaata gccaaagaaa taaatttcaa tgataattgg tctaatgaaa taaaagaaaa
     2581 tgtacttgaa atagttgaaa tatttgacga cgcagtaaaa caagctacta gcacagaata
     2641 tatagagaaa gctaaaaaat acataataga aacattaata caaaaaaatg cacaatttta
     2701 tagaaatgga tttgcagaca ttaaaaagaa gcagattgaa aactatagaa aaatagaaag
     2761 tgctgaaaga aaaatagaaa ttgcatacca aatatttaga gatggtgtag agtgttaaaa
     2821 attttggatt ggtacaaata tagttcaccg tcgaatgcta taattttaat atataaaata
     2881 aatggaggat tgaagtatga atatagattt tagtacaata ttaaatttaa aagttaataa
     2941 agagaatgtt gaaatgatag aaaaagagat taataaaatc aacaatgata tatttgatat
     3001 aagtcttaaa gattgtgcaa acggtcacgt gggaatcgtt aaagaaacag ttggaaaaag
     3061 tttaaacatt gaaacgtggg ggcatggtga aattaacagt agcataggaa taagattgag
     3121 atttaatttt tattattcaa aaggcgacaa agtagacata gaaaatatag aaaacatttt
     3181 aatatatgta aatagcttat gcagcaaaaa tgtagaaagt gaccttatag ctagtgtaag
     3241 acctggattt tataaattag aagagaaata ttctacacgc attgaaaaat gcgaaattag
     3301 acacgaattt acaggcataa acaaacaaga aattaaagaa tttttagagt atcttacaga
     3361 aattgaaaag agcgaaagaa ctgttatata taaaaaataa ctaaaaaatg gaggatataa
     3421 aaaatgaata aacaaaaagc tagaagattt ttaagagtta tagatatgaa catggataaa
     3481 atagaagaag aagctataaa agcttttaaa gaaagttgtt taatcaaaga gactaataat
     3541 ataaaaattt atatcgatat acaaggaaaa gttgaagcga tagcagttca aacttgggct
     3601 aaacttttag acgacgacaa agaaattaat attttcacat taaatcaaac accaactcat
     3661 ttaaacgatt tgtttggaga aatttgttac gtaaacgatt atgaagaatt tgaaaattgg
     3721 tgtgaaaacg agcgggaaat tttggattgg gatagttata aaaaattcaa taaagaaaat
     3781 ttcgaagaaa ttgctgaaag aaatatagac gatagcacat cagttttttt agaagaatta
     3841 caaaaaagca ttgaaagttg taaacaagaa ttacaaaata tagttgaaaa ttaagtagaa
     3901 gagtattatt attaatgtat atagtatcgc ttttaagatt aactacgagg gatttaaaat
     3961 attttttaat ttatttagaa ataatattgt aataagaatt tttatattaa ctacaaggga
     4021 tttaaatgtc aatttaattt atagagaaat gatataatag tattttttat attaactata
     4081 agggatttaa atatcaattt aatttatatc gaaataatat aatatccaat tttagattaa
     4141 ctatacggaa ataaaaacag tgtattcaat aaatacactg ttttttaatg tttagttatt
     4201 atgataactt aatccttgaa ttataaatcc tacattcgtt gtcatctcta attcgttact
     4261 taacattatt ccttgtgtaa attcattgtc agctggcaat gttactcttt ttctagcaac
     4321 cgcttctata attgtttgaa atagtttaat tttcatatca ttatcatgtt cagcgaatcg
     4381 tagataattc gttacttctt gcgaaaactt attcaactcc gatttagtca ttttttcaga
     4441 taactctttt ccaatttgca gataaacatt catcttttcc aacccctttt tagattatag
     4501 ttaaaacata ttatttaaac aatattaata tattgtaata ataatattaa aatattactt
     4561 aaatattata tatacaatat ttaagtaata tctaaacaat ataaatataa tattatttac
     4621 tttttcttag tcttatttct tctatttctc tcattgtata tatttaaatt ctcttctttt
     4681 atagtgacat cagataaaat tttagttagt atatcactta ataatttgct aacgtcgctg
     4741 ttcatatcaa cagaaaaaga ttttaatttg tttacaagtt cttcatcaag cgttgcactc
     4801 atagttacct ttttactttt cttattaact agattatata tgtttaatgt cttattgtct
     4861 gtagttgtag aatcattttg tacaattgtt ccagttttca aaatcggttt ttcgttatcg
     4921 actaaaaggt ctctgttgaa acttggcatc tataacactc cctttctttt caattcttct
     4981 aacatattag taaattcttg tctgctgaaa tgctctttag ttatttttgt ataatctgtt
     5041 agagatagtt ttttcaatag tgccttctct ataaactcgt tttttctaat atatgtatct
     5101 aacataatat ctctcatatc gttaaaccct tccagataag aatcgaaagt atcactaatt
     5161 tgagttttct tcttctcaaa tccaactaaa acagtacttt ttatattatc ctccttatca
     5221 aaataggttc tatcaacatc ccagagttgt ttaaataatt ctgctcccct taaagaagaa
     5281 atatttttat cttgaattag aataattata ctatctgcca agaaaagtac atttttagct
     5341 gtcaaatcgt agcgtggaga taaatcacag ataatataat catactcact caaagtatta
     5401 aagttttgca tgtaccagcg agctaaaaat ttctctctgg ctggtaaagg tgatagctct
     5461 tgttcaaatc tagccatttg tatgtcagaa ggaattaagt ctacattagt gtaattttta
     5521 ttaggcgact tgactattgc atcattagcg ctaatccctt ttaaaatatc taatgtagta
     5581 ttatcattat gatttatttc ataaacatat tgtgttagat ttgcttgtac atctaagtcc
     5641 cataagagta tttttttatc tttatctttt gcaagttcat acgctgtcat tatagacgtt
     5701 gttgtcttat atattcctcc ttttatgtta aagtacgtaa gtagctttgt attagccatt
     5761 ttctccacct cattattata tttacaatat tataataata tcatagtatt gtttttataa
     5821 tattacaaca atatattaat attattaatt taaatattta aataatataa atacaatatg
     5881 taaatattgc attcataata ttaatatatt atattcacaa tattaaaata ttgtatatgc
     5941 aatactaaaa taatattttt ataatattac agtattattt ttatattata gtaaaattta
     6001 aaaaagaagg ctaagaaaaa agcaaccttc tttttataga aaaagtcatt cattcttagc
     6061 cttttcaatt attatcctgt cgccctcgaa tgttgccgta acattagggt tttctcttgt
     6121 tatgcccatt tcatcagccc attttttggg aacactcatt ctaggagtaa acgacccact
     6181 cccaccttta tgaaagttaa actttaaatc tcttttttct tttttcattc attagctcct
     6241 tttttgctgt attacaaaga aaagtaataa tacaacacta aaaactaaac cgagtatgtt
     6301 gcttgtacta gagtttaata ctatagatac tatatttaaa actatagtta ttaaaactaa
     6361 aacaatacaa atcttataaa atttatcact catgtttttt tagaaatgtg ttataatatt
     6421 agaaagaagt cacctagaga gaagggctat tctctctagg cttatcctta caactctttt
     6481 aaaattctta gaattgcaag gattatgtta agtaataata agatgaagga gaaaactttt
     6541 ttaactatgt taaatttgtt ttctcttttc tttttacttc tttctaatct ccccattcct
     6601 gtgcctcctt tcttttattt ctttttactt tctcacctcc ttctctataa atatattata
     6661 tcatgacggg gcgtcaaagg caatagtttt ctcctatttt ttaaacaaat tttggtaaaa
     6721 agtaaagatt ttagtatatt agaaatgatg taaaattgta tcttttttaa aaaatgtgat
     6781 ataataaaag caaggaaata atgatttact ttacacaaga gtagctattt ccaatcagat
     6841 taaaaataac ttattttctt aaaccactct tattggcgtt tgagtgtttt ttaattttat
     6901 cgttcttaaa ataaactttg ataaaaataa aaaaatagaa tactgaaaat gatgtaaagt
     6961 tgtatctttt aaagaaaatg tgatataata aaagcaagag aactgtattc tagtcgtaag
     7021 agtagagttt tcgtctaaag taaaaaaatt atctagactt tttgaatttg atttttaaat
     7081 caaattccca accactcttt tgcgcagagt ggttttttac ttgtctaaat aatttactaa
     7141 ttatatagaa tattaaacta gctataacac cagctaatat gttaagtaaa aaattatcca
     7201 tacatattca cctcccctca tagcgttggg aggataatct tttgtgtata gactccactc
     7261 tttttttaga atatagattg cttctcttgc taaaattatt ataacatata tttacataaa
     7321 aaaagactta gtaagtgtac taagtctttt aaaatacagt tttttagaaa atatattttt
     7381 aaaatataat ttattttcaa aatcaatata tttttttatt ttatatttat gctataatta
     7441 atattaactt attacagatt acaaaaacaa ataaaaaagg aataagcacc aagtcgccaa
     7501 actaacactt attccttacc acggagcttg aagctcttat aagatataat tatatctttt
     7561 tttaactaat ttataagtta tattatagat taaaacaact tataaatcaa tgttttagag
     7621 ttaaaaataa tttgaaattt ttaacttaat tagttaaaag tgcttcaagc cccgtttaga
     7681 agggggaaat aaaatgtctt tagaagaagc aaaagaatta gaaaaaaatt ataaattacc
     7741 aatgccaact ttttgtttga aagaaaaagg atattttgca agagaatacg ccgccttgat
     7801 gttattaagt aacggaggaa tgaaaattat gcatacagaa aaaaatagat acttatatca
     7861 aaataaattc gatatagatg cattagcaaa acagctaagc atttcaagag ctacattata
     7921 cagaaatatt aaaaaattag aagaacttaa atgtggtatt ctaaaaatag aaaatacgaa
     7981 aaatggaact gtatacagac taaaatatgg aattgaaaca ggttataatg acgaagtgca
     8041 taagtttgtt acaatacatc ataaaatact tgaagaatta gtaatcaatt tcaaaactcc
     8101 aagcgtaaaa ctttattgtc ttttatgtta tttaacaaat gaaatagatt ttaaagttat
     8161 agacgaagaa tttttatgta ataatattgg tctttgtggc aagagcgaaa gtaataaaaa
     8221 caaagttaga agtatgatac gagtattaat gaaatgcaaa tacatagacg taaaaaaaga
     8281 gaatagaact ttctggaatg aagaaacaaa aaataaaatt aataaaacac taaaattata
     8341 tagattatgt agttacgaag agtggaaaaa cttaaatgat aaaacagact ttaaagatta
     8401 aggtctgttt ttttgtggat aactatgtta ataacctgtt gataaaatgt ggacaactta
     8461 ttgtttttta attagaaatc taacttgtct caaaaagtgg tgggtgcatt ctcaaaaagt
     8521 ggtggtcgca ttctcaaaaa gtggtggtct aaatatatag agaacttaat aaatagaaaa
     8581 cataaatatg ttttctattt atttctttat ttaagaaaat cgaaaactta aaaattttta
     8641 atctatgtaa ttagaaatct aaccggatgc aaaaagtggt ggtcgctatt taaaatatta
     8701 ttagcatttt tcaaaagtaa agaaactctt agaatgtatc tttagataaa aagaaagtga
     8761 ttatttaatt aagagactag aatatcattt agaagcgata caagacacca gaaacgattt
     8821 ttctgtttgt tataaataat ttatgttaaa tagcataaaa aaatgcttag aatggaaata
     8881 aataggtatt attcttttaa attattttca tactactaga atagaaaatt aaaggaatat
     8941 aagttttgat gttgaaatat ataacaaaac ataaaaacat ggaggaatcg aggatgaaaa
     9001 aagtattatt attaattaca gtatgtttat taagtgtagg gttggttgct tgttcatcaa
     9061 ctaataacaa agatactgga aacacaacta aaaaagaaaa gaaagcaaat gaaaatgagg
     9121 ttgatggaat taaaataact gtaaaagatg ttacaaaaga agatataaaa ggcgatataa
     9181 acgaagacgg aagttttaac gaaaacgggg aatattttag tgatggaatt gaaaagaaaa
     9241 aggcagctga ttatgtgtac gaagttgtaa atgtagaagt agaaaataaa acagataaag
     9301 cagttaaatt atttcaaaca ggatggaatg cagtcggtac agatggttac gaatttaaag
     9361 acataaaagt aactgataaa ttagacaatc aacaagtacc agctaactac aaatttgatg
     9421 cacaagttaa aatattagtt gaaaagaata tgaatgtaaa agaaatagtt ttaaaatata
     9481 acctgaaaga ttattcaaga cttttcgagg caatggagta cgcaaatcaa ggagcatcaa
     9541 aatcagatgt agaaaagaaa tttccggaat tatataaaga taactggatt gagctaggag
     9601 aaattaaagt agagcagtaa aaaaaagggg gctaggtata aataattacc tagtccttat
     9661 ttttatattc ttttaaaaaa tccctgctaa gtgttttaat tgctttttta ttatattttt
     9721 tagtattttt taaatgctcc ctttcttttt ccaaaaaaaa ttcagataaa atcaattggt
     9781 caatttctaa tatatcagct aataaaatta tttgagttaa ggttgggctg tgtttaaatt
     9841 ttttgttttc catccttgaa atgtaacttt gacttattcc tgtcaattca cttaatcgac
     9901 gttgagtgag ttttttcttt tttctatgat attttaataa agactccatg tcgaaattaa
     9961 actatagtat taaaaaatat taaaatcaat agaaattttt tacaatttag aaaatagaca
    10021 aattaattgg taaattttgt aaattaaagt cggaatattc gtcgtacgaa tatttttgga
    10081 atggttttcc gtgttaatat tagagtgtag aacaaaatat aagctctagt actagagctt
    10141 attaattcta acatattctt tttctaatat agtaaataca atatcttttc catgttcatc
    10201 gagagcatta tacattttta aaattttttg ttcgaactga tttgtttgta tctttgaaga
    10261 tagttctttt tcttttagtt cgttgtcaat aagagcatca gcgtcaatat ttaaagcgtt
    10321 acaaattttt aatactgttg ttatactagt accacttatt ccgcgcttaa gcatagtatc
    10381 aactgtagaa taaggaatat taacttcttt tgtaaaagct cttatgcttt tatatttgct
    10441 aagtattaat tgttttaaac gttcttctat attcatttaa tcaccacctt gtttttaata
    10501 taatggtaaa atcgtaaaaa ttcaagaaaa atttacgaaa ttgcaaaaat aaaatacaaa
    10561 aacatattga ctattcacga aattgagtgt attatatgaa atataagaca cgcaatttcg
    10621 tgaataaatt cagaaaggag cattaaaatg tttagaaatt taaaagcaga aatggcgaga
    10681 aaaaatataa caaatgaaaa aatagcaaaa gcgttaaatt taaatgtaag tacaatttgc
    10741 gccaagttaa atagtattaa tagactaaaa tatattgagg caaaaaaaat acaaacaata
    10801 ttcttccctg atttaagtac cgaatatctt tttgaaattg aagaagaaaa aagtaaaaaa
    10861 tatatatgaa agaatcagaa tattcgaaat taagaggtgg atagattgaa attactaaaa
    10921 agtaaaaata taaaaatagg cgaaattatt agattgcatg atgtgaaatt taatcatgat
    10981 agaaaatgta ttgttacata tatagataaa gacgaaatag tattaacgta ttatgacgaa
    11041 gaaataaaag aaatgacatg caaagtatta actaaagaag atttgatatt taatgactat
    11101 gaacttaagt tgctatctta ataaataatt tataagtgga aggggggtga gggattggaa
    11161 cttttaaaag attcaaggaa aagagattgg ttttgggcag aaaatagttt agttgataga
    11221 gaagatttaa gcatatacga aaagatgatt tatatagttt taattagata tagcgatgaa
    11281 gaaagttgtt gttttccaag ctataagaca atatctaaaa aagccagatg cagcgaaagg
    11341 caagtatcaa atgttttgaa aagtttagaa gaaaaagggt tacttataag aaaaaaacgc
    11401 aagcgaaaag gtagtaatga aaaagaaagc aatctatata tattattatc agctaaacaa
    11461 gaggtgcagc acacaatgcc taacgttagg aatgaggtgc ttaaccctac ggcatgtggt
    11521 gctgtacatg ttaggaatga ggtgccgagt aacaagacta atattaacaa gactaattta
    11581 actaataata tatatagttc tgcagagcaa aaagaagaaa agacaaaaga caaagagata
    11641 tataaaagaa tcgttgatta cttaaataaa acagcagata aatcttttaa gagtacaaca
    11701 aagaaaacta tatctttgat tgatgcaaga ctcaaagaag gctttacaga agaagaattt
    11761 tacaaggtca tagataacaa agtgttaaat tggctagata atgataaaat gaacgcttat
    11821 ttacgccctg agacgttgtt tggaaacaaa ttcgagtctt atttaaacga gacacctaaa
    11881 aagtctctaa aaggcaatga gagcgtcaaa ggaaaaacaa agacaaataa taataaattt
    11941 gctaatttca atcaaacatt tgataaatat tcagaagatg aacttgatgc aattataaag
    12001 aaaagtcaaa gtgctaaatt taaataaaat taaaaaaggt ggggtaaaaa aatggaaatt
    12061 aatagcgtaa gagagttaag agaattgttt gaacttttta cagttataaa agcggaaggc
    12121 aacaatgttg tttcttgcaa tgaaactgta aacgagttaa gtaacaaaga actaataaaa
    12181 gcaaatgaaa acaaatttaa aaaaccttgg aatgatgctc tattatatga aataaatgct
    12241 ttagatataa taaaaaagca taagcacagt gatagaaaag aaaaggcata taaaacttat
    12301 ctaggattag aaaatgcaga tatagaagaa atttataaaa ttcattatgg agataaagaa
    12361 aattctgaaa ctgttgcaag ttgcgaagaa attagttcag caaaaacgga tacaatatct
    12421 tgcaatcatg acagtgtttc aaaaggtaaa gatactagtg attcagaatg tacttgtttt
    12481 atttgttcta aaaaaataga aaatcaagaa gatgtattta aaataccttt tttaacacga
    12541 gattatttga gttcagcaaa agaaataaaa aatattgaaa tttgtgagaa atgtttggat
    12601 ggtaattcgt ggagtattat aagaagaaag cagtttaaga aaatgaagaa ataaatattt
    12661 aaattagtat ctttacatgt gaaaaaggag agtgaaaaaa tgataaaaaa taatgtttta
    12721 agttatgaag aaaaaataaa agtattaaaa aaacatggtt atagtgattt taccaaagaa
    12781 tatgttttaa aagaatttga caaggaacat gcattttaca aatcattttt aagagcaata
    12841 gaaaaagaag atgatggaaa aggtgtgtta attaaaaaaa ttggaaaagg tcagcatcaa
    12901 ttttcaaaag gttttataaa aacacacact atcagtgagt tattggaaga agcaaaacgt
    12961 attagaaaaa tattagaata aggcttagaa atgtctataa tctaaacctt atacttaaat
    13021 taaaatttac ctaatatgta ctctattgcg ataggtaggg acatatttaa aaaacttgaa
    13081 gtggaacttt ttattttatt ccatttagtg ttatctcgta ttttatctaa atattgataa
    13141 ccttttaaag acaactgcat gacctctaaa acagtatcat caccgtaatt aacatcgtca
    13201 acgagattat tttgataaat taaatttatg tgatgaacaa tagttgactt atcgtatgtg
    13261 agattatcta gtatagtatc aaattgtaat acaggcgaaa tgggagttgt attagattca
    13321 ataagtaata aaatatctct tatacaatct tggttagtaa ccatagtatc acctcctttt
    13381 gctttagtat agcacaggag aaatgagatg ataataaatt ttactgtagc agaatgagaa
    13441 ggagcaaagc aaatgcaaaa gagaattaca gaaatgagta tattagaaaa agttgattta
    13501 attggacaga aacaaaagaa actacaagaa aaatataaaa atctaagtaa gggagaattg
    13561 ttttctattg cttgtaatga agttaaaaaa atagaagagc aggagggaaa atattttgaa
    13621 tataggtagt tatacattag ttaagaaaca actaattgaa gaattgaaag aagaaattaa
    13681 gcaaaaagaa gaaaacttaa aagaacgtga gaaatacata aaagaattaa aagagaggtt
    13741 agaatgtttt gcgagggatg cgttagaatc agaagagtgc ataatagata ttgaaaatat
    13801 tttgatagaa caaactgtag aattagaagc ttataattta ttcatagatt gcattttaaa
    13861 caaagatata agaaaagcta ttgcgattta taacagaact aaaagcatca gaattaagca
    13921 taaaaatatt aattctatat ctaagaaaat tgaaaaaata aaacaaataa tttccttgta
    13981 gaaataggag gagtttaatg atggataagt ttgtaacatg cgctagatgt gaaagaacaa
    14041 taaatgtaga agaaaataat catgtgaagt atgaagaaga aactttaaat ttaagtttta
    14101 cgttgtattt ctgtttaggc tgcgtagata aattaattga acaatctaaa atgttggagg
    14161 gagaaaatga ataaaattag cgttacacaa gcattagaga aatttgacag tttactagat
    14221 aattggaacg accttccaaa tcatgtttac acacttgaat atagaagcaa attttacgaa
    14281 tggataaagt tgcttgaaag aaaaggtagt ttacaaaatt ataagatagt tgaagtttta
    14341 aatgctgaaa gaaatggaga agaagcgtcg ttttggaatt gaagaaatat aacttgaaaa
    14401 ggacttaaac tttttctaga tagaagggag gtatataaat atgaaaatga taaatgtttt
    14461 taaattatca tcaatgtatt gtatttgccc caaatgcggg agcgataaac ttggagaagg
    14521 tgaaggaaag ttaattgttg atgaacatac ttattacagg gaatgtaaat gtggttttaa
    14581 tgttcttgta gatgaaagag aagacgaaat ataaggaagg tgctggaaat gaatagtgtt
    14641 attttaattg gaagattaac aaaagaccca gaacttaaat atataccggg ttctggaaca
    14701 gctgtatcga cttttacaat agcagtagat agagattata ttaaaaaaga tggaactaaa
    14761 gaaactgatt ttatacctat tgaggtgatg gggaagttag cagaagtatg tgctaataat
    14821 ttaggtaaag gtaggttagt agcggttgaa gggtctataa aagttaatag ttatgaaaaa
    14881 gacggggaga aaagaactta tacaaaagtt catgctaata agattaagtt tttagactat
    14941 aaaaaagatg atactgaaaa agaatatatg tttgaaccga aaggtttaga ccaacaagga
    15001 tttcaagcta tagatgaccc aaatatacca ttttaaagtg ggagggataa caatgtctaa
    15061 tacaaataaa aaaacagcta aaatttgcgg atggtgtgga acaacagttt attcttatat
    15121 ggatggtaaa attgcatatt gttctaagaa atgtaagaaa aaaagagaaa aaaatttaga
    15181 aacagaaggg gcaaaaaaat gaaagagtat agaattgaaa ttggagaaat gatattttta
    15241 aaacataaag ttattgttga agttccagaa caaatatgcg cttttgatat atataaatat
    15301 atagaaaatg aatgtcaaga taaaagtgaa atatctgaat atataaaaag tattggtggc
    15361 agacaattaa attttataga aggagatagc ggagacagcg agttaatagt tggaagttgc
    15421 aagaaaataa aaaaaggaga aaaataaaat gaaaaaaaca aaaatcgaag aatttattac
    15481 tagagttaaa gaaagtatgc aaagcgtagc aaaagaaagt ataaaagact tagaaaatat
    15541 tttaaaagaa attgaaaccg gagaaacaga agttaaaatt ttatttattg acaagggaaa
    15601 aaaagttgaa gagattgttg aatttacaca tgacgaatac gtgaaaatga ttattgaaaa
    15661 aatgatattt gatagtctta aaataataaa agaacaacac gaaaagattt taaaagagtt
    15721 cgaagaaaga gaggtctaaa aatgaatttt aaaatgattg caaatatacc aaagtggaca
    15781 gtaaaagaac aatttgaaaa agtgcttgaa gaagttttag aacttaaaga agctattgaa
    15841 ttaaaagata acaaaaaaat aatggaagaa ggtttggatg ttattcaagc aatttttaca
    15901 ctatttaaag tattaaagat tgataaagaa atacgcgaag gtttgaagtt gcataataaa
    15961 aaactaagaa aaagaaagtg gaaactcgaa aaaataaatt agcagaaggg ggagttaaaa
    16021 tgagtatatg tagaaagtgt ttgtacggag actatttaga tgaactagta gtaggcgaat
    16081 gtattttatg tggtcaagaa tttttttcta taaaaactcc ggttgggaaa atgtgtccta
    16141 gctgttcaga aatgtttaag gggaattgtt ctcaatgtgc aaaaccaatc ggaaatgaaa
    16201 aaaagtcatt taaaaataaa ttaaagtaaa taggcaatat aaaacataat ttgattgatt
    16261 tttaaattaa aatagataat atactttata aatatagatt ttatttaaac ttaaaaaaat
    16321 gaagtcttat gacaaaaaaa caaaataaaa atccagaata ttttgaataa tatgcagtag
    16381 ttggaaatgc tacaaaatac ctaactagtc ccaaaagcta aatttaagga ttagttaggt
    16441 atttataaca aaaagtacaa gaggtgtaaa aatgaaaata aaaaaagagt attttgacaa
    16501 agtggaaaat gcattaaaaa tgttaggaga taaatataga aagttaagag tattaaaaaa
    16561 agaaatagca attcttaaac acaaagaaaa ctatagagaa attaattatg aagaattagg
    16621 gtttaaagta caaaaaagtg taaaaggtat agatgacatg gttgtaacaa tagaggatgc
    16681 tatctacaac aaagaaactg aaattgaaat tatagagcga aagctagagt tttataatat
    16741 ttatttaaaa gagctatcag agatagaaca aaaaattata gaactagtat acttttatag
    16801 ctggaatggg aaaatgccta ttacaaaaat ggcgatggaa ttaaattatg atagaactag
    16861 tctttataac aaaaaagcta ttgctattaa taaaatagct ttaatgatat atggtgatga
    16921 agctttagaa taaacattat ttttacagaa aaaagactat ttttatactt cgtttctaaa
    16981 gaaagcatgt tagaattgta tcatagaaaa aagtgtacat tgacaactga atatcccccg
    17041 aaacctaaag tctaaacttg aagctcgaaa gtttaggctt ttttattgct aaaaaaggaa
    17101 gggggtgagt aaatgaattt tgtagaacct atcagaaatt tagagaagct agaaagcata
    17161 tgcgcttatt tagaagaaac taacatgaga aacaatgtac tcttcagtat gggcatatat
    17221 acaggactta gagtagggga catattaaag cttagaataa aagatgttca tgacaaacgg
    17281 tttataactc ttaaagaaag taagacgggt aagactaagc ctatcgaggt taatcctatt
    17341 ttgagaagaa tactaaaaga atatacaaaa gataaaaatc aaaatgaatt tttaattaag
    17401 agtagagagc agaccaataa agctataagc agagtcatgg cttataagat aatacgagaa
    17461 gtaggcgaaa tgtttggtat agataatcta ggcacacata caatgcgtaa aacttttggt
    17521 tatcattact ataaaagaac acatgatgtt gtaacattgc agaagctttt taatcatagt
    17581 tcagctacga taaccttgcg gtatattgga attgaacagg atgatttaaa caaagcatat
    17641 aggaatatta aatactttta gcttaagagt ttaacataaa aaggtcacgt taaactggat
    17701 aaaatatagt ctaataataa agtcttgaat gtattgttaa tactaaagtg taaggctgtt
    17761 tgataatact taaatgagtt taacagaata tatcttatgt taaactcaac taggaaatta
    17821 aaggagtatc actatgaata agtatggtaa gtttaaatgt aataattgcg gaaagaaagt
    17881 taatgtaaat aatagagatt taaagactaa agttattgat aatactactg ttacatactt
    17941 catgtgtcgc agatgctttg agaagtttgt tgtatcatgt gaggatgatt atattaaaca
    18001 taagagagaa gaatataaga agcttaaaga tgatgaagtc aagctattgc aagacatgaa
    18061 agagtatagc gacggtctag gagagctgta tattaatgag ttataagcac atgacactag
    18121 atagattaaa agaattaatt ttaattgata gacttgatat attctataga agtacagact
    18181 ggcgtaaatt aagaaaaaaa gcacttgaaa gagataataa tgaatgtcag atttgtaaag
    18241 aaaaaaagaa agtatctaag gcggaatgtg tacatcatat caaagaggta aaagagtatc
    18301 caggtgaagc tttgaagctt aataacctaa caagcttgtg caacctttgt cacaacgaaa
    18361 ttcacgaaaa aatactaaaa attaatcaag aaaaaaagaa aaaatttttc agtgaagagc
    18421 gatggtagat agccccccta ttgaaaaaac tggctttcaa ggcaaaattt tgtgaaccga
    18481 ggtaggtaca tggctccgca aggagacgtc aatttaacaa aaggtgtggg gggtgggggt
    18541 cttgcaacca aaattatggt tgcgggtgca gaaaaaaaca taaaaatgag gtggtgaaaa
    18601 tggatgataa tgaggaatta gaaattaggc gagacgtaca gaatgactac gttttaggca
    18661 tgacttacaa agaaatgtca gccaagtata acatacccga aaacactttg aaaagttgga
    18721 gaaaaagagg gggttggact cgcaactgca ccccaaaaaa gggttgcgtg aaaaatgggt
    18781 tgcaagagtc gggaaataat ttaaaaaact ctataagaaa agatttatta agacaaataa
    18841 aagagaataa catgacagaa gaatacttca aagacttagt tgaagattat ttaaaaatgt
    18901 gggatataaa aaatgaatta atagaagatg taaaaactag aggtgttcaa gtcaaatata
    18961 aaaatggtca aaatcaatgg ggctataaaa aaaacgatag tgtatcagag cttattaagt
    19021 ataacgctca aatgttaagt ttacttgata aactaggtat aaaagcgaca ccaaaagttt
    19081 tgggtgatga tgatattgaa ttataagtac aataaataca tagatgaata tttcaactta
    19141 gttgataaag gcgaaattgt tctatgtaaa gaaatggaac ttgcagttaa gaaaataaaa
    19201 aaagatttaa aaaaacctaa tatctatatc gaccatgaaa aagttgaaaa agctatcgaa
    19261 tacatggaaa agtatttcta taaaatgtat gcttgggaaa aatttgttgt tgctttagta
    19321 gttggctgct ttgaaggcga cgaactttta tatgatgaaa tttttatcat gatgggcagg
    19381 ggaaatggaa aaaatggttt tatatctcaa ttagctaatt atttgcaaac acacttacac
    19441 ggtgtaaaaa attatggtat tgatattgtt gcaatgtcag aagaacaagc aaaaacaagt
    19501 tttgatgatg tttataacat gttatctgaa catcctgcta tatgcaaaaa gttttttaac
    19561 agaactaaag aagtaataac atttaaaaaa actaacagct atttaaagtt ccgcacaaat
    19621 aatgctaagt caaaggatgg attaagaccc ggctgcatca tttttgatga ggttcatgca
    19681 tacgaaaatt atgataatat aaaagttttt acctctgcac ttggaaaagt aaaaaatcct
    19741 agggtttttt acattacaac tgatggaaat gtaagaggtg gggtattaga cgatttcaaa
    19801 gaggaatcaa aacaaatttt aaatgaagaa attaaaaact ctaaaatgct gccacttatt
    19861 tttaagcttg atagtataga agaaatggaa aatattgatt tactggaaaa agcaaacccg
    19921 agcataaagt ataattcaga cttaaggaca caaattttaa aagacattga caaagctaaa
    19981 aatagaccgc agatgatgat agaattacta actaaaagat ttaatttgcc agcgcaaaat
    20041 ttagcgactg ttgttgctga atgggatgac atagttgcaa caaacgaaga attaccagac
    20101 ctaaaaggct tttcgtgcat tggaggatta gatttttcta gtataagaga ttttacaagc
    20161 gtggtattgt tatttaaaaa agataaaaaa cgatatatca tgcatcatac ttttatatgt
    20221 tataagtctt tagaaataac aaattttaaa tttgatattg aattagcaaa gcaaaaaggt
    20281 ctttgtacaa taatttatga tgaaacgata aaagcagagt atattgctga ttggttcatt
    20341 gaaaaagcgg aggattataa tatattaaaa atctgctgtg actcatatag agctgcacat
    20401 gtaaaagaag aatttgctaa caaaggttta ccaatcgaag aggttagaaa tggttcaata
    20461 actcatgcga aaatagcccc tcttgtagaa caaatcttcg cagagagaac aattaaatgg
    20521 ggcgacgaca tgatgatgcg ttggtatact aataatgttt atgtagatac agacaaaaaa
    20581 ggaaataaaa cttttttaaa gatagagcca atcaaaagga aaacggatgg cttttttgca
    20641 tttttacatg ctttatcaaa agatggtgaa ctgatgcaag aatcaacgat taagttttat
    20701 gattgttata catattaaaa ataatgaggt ggtgaattga attttaaaaa ttttataact
    20761 aattttttat ttaaagataa aatgtctgga gcagatggag agtattttaa aaaactagaa
    20821 gctacgatat tttataaaga atttgcatta aggtcatgta tttctattat tgcaaatgct
    20881 ttagttttaa gtgaatttca gacgtttata aattcaaagt ctgttaagga aaaaaattat
    20941 tacttgttta atatagaacc taatcaaaat caaaacgcaa cagaattttg gcaagaagta
    21001 attacaagac ttgtgtatga caatgaatgt ctagttatac aagaaaacga acaacttttc
    21061 gtagcagaaa gttttaatgt agataagtat gttttttatg aagatgtata caaaaatatt
    21121 acaattagaa actataaatt agatagaagc tttagagagt cagacgtttt atattttaaa
    21181 ttaaatgttg aaagcataaa aactgtaata gattatcttt ataatgatta taaagattta
    21241 ttaagtagcg ccatgaaggg ttataaaaaa aataatagcg aaaagggcgt acttgaaata
    21301 gacacaaatt acccacagac aaaagaagca caagaacgac ttaatgattt aatgagtaat
    21361 aagtttaaac gttattttga aagtgataat gctgttttac cgctttcgaa tggattaaaa
    21421 tatacagaaa atacaaaagc ttcaagtata aaagatagca gagatataag agcgattatt
    21481 gatgatgtta tggactttgt atgcgctgct tttcatattc ctgctgggct tataaaaggt
    21541 aatgtttctg gcgttgaagg tataacagat aattttttaa acttttccgt caatccaatc
    21601 gctcgactta taacaactga aataactaga aaaatgtatg gtagagataa attttttgaa
    21661 aagagttatg taaaagtaga tacacaaaaa attgctaaca tgggacttga aaaaatatca
    21721 aaagcaagtg acttactgtt tagaataggt gtaaattcta taaatgacaa tttagaaatg
    21781 cttggtcgag aaaaaataaa tgagtcttgg gctgatgaac attatgtaac taaaaactat
    21841 cagtcgattt taaatccgga tttgaaagga ggggctaaca atggaaatgg agaaaagagc
    21901 ggtatcgctg gaaataaggg acagtaattt agaaagtcgt aagataagtg gttatgctgc
    21961 tatttttaat gatgattata ctaaattgca agacagatgg ggcgatactt tttacgaaaa
    22021 gatttcaaga ggtgcatttt taaaaacttt agcagataac actagggata agtttatgct
    22081 tataaatcat gactggaata aagttatagg tagaactaac tcaaatctaa ttttagaaga
    22141 agatgaacgt gggttaaggt ttgaactaga tataccagct acgacggacg ggaatgattt
    22201 gcttgaaaat gtaagacttg gactaattaa agggtgttct tttggtttta atattgtaaa
    22261 ttcaaaaact cgctttgatg atgattggac atattacaga gatataacag aagttgaact
    22321 atttgaaatt acagcaaccc ctattcctgc ttataatgat actgaaataa attgtagaag
    22381 tgatatatct ataaaagaac ttagagaatc tcaaaaagaa aaagtaaaac aaaatgataa
    22441 taaagataat atagaaaaaa ggaatgtcga cttagtatcg gcttttttta atgcatttaa
    22501 tcgaggagga aaataaaaaa tggcaattaa aaatttagat gaggcaacta atgttgaact
    22561 aagaacgaat ttaatggaag cgataaagag taatgacgaa gaaaaaataa caacatcatt
    22621 tttaagaatg gcggaagata tacaaagtaa tttactaaaa gaagcacgtt ctattgctaa
    22681 tatagaatca gctgacaggg cgatacttga caagaggggt atgccacaat taacaagcga
    22741 ggaaagagca tattatagcc aagtgataga aaaaagagga tttactgatt tagaagtaac
    22801 tatgccgcgt actatatttg atagggtgtt tgatgactta gaacaaaacc accccctact
    22861 tagtgcaata acttttgaaa atacgacagg ggttacggag tgggtaataa gaaaaacagg
    22921 aacggaggcg gcttggtggg gacaattaac tgaccctatt aaaaaagagt tagaaggtgg
    22981 atttgcaaaa attaatactg ttgcttataa attatcttct tatcttccag ttgcaaaatc
    23041 tatgcttgac ctaggtgctg aatggttaga cagatatgta agagtagttt tgactgaatc
    23101 tatatcattg gggcttgaaa tgggaatagt agcaggaaca ggaaaagacc aacctattgg
    23161 gatgatgaag gacttaaaag gctctgttgt tgaaggtgtt tatccagata aaactgcaaa
    23221 accattagct gattttaaac caactacatt aggaactgaa attatggcac cgcttacaaa
    23281 tgatggtaaa aggacagtcg caagcgttat aatgcttgta aacccggttg attattgggc
    23341 taaaatcttc ggtgcaacga ctatgctaac ttcaaatggc acttatgtat atggagttct
    23401 gcctatacca ggaaatataa ttcagactgt tgctgttccc caaaataaat taatagtagg
    23461 ggttgctaaa gattatttta tgggaattgg gtcagctggt aaaatagagt attcagacga
    23521 atataagttc ttagaagatg taagagtcta tcttgctaag cagtacgcaa ctggaacacc
    23581 aaaggataat ttttcttttt tagtatttga tatagaaaac ttagacacaa ctttagcaac
    23641 agaaattaat tcaaacacaa aaacgtcagc tgctagaaca aaataggtga aatatatgga
    23701 aaatgaaaac attaaaagta tagttgaaga ggtaaaagaa tatcttaatg taacttggat
    23761 agaagaagat gcgaaaattg aaaaaatggt tgtaagagga attgattact ttgaaaatga
    23821 aatagcagga atagcactag attttaaaga taattttaat agagaattgc tatttaatta
    23881 ttgcagatat gtaagaaaca atgctattga atatttcgag gaaaatttta gtaaagagct
    23941 tcttagactt caaattaaaa gtgcagctaa tgaggtaata tagatatgaa aaaaataaaa
    24001 tttaataatt ttaatgatgg tattgttgag tttggagaat acaaggaaag ttatgatata
    24061 gaaggtaatg cgttagatga aaaagagttt attgtaagtg gtaaactctt ttattctaat
    24121 gaatatataa gagagcaaga caaactcaaa tttgataata cagggttaaa agtatctatt
    24181 aagttaaaag taccttatat gagattaatt aaaacaagtg atgttataaa gttaaataat
    24241 gaattttaca gtatagtaaa acttgatatt agtaatgaca aaactagtat atatttatat
    24301 ttatctgaat tagttgacgt actagataag catatttcta tttttatcaa agagagaaaa
    24361 aatgttcttg aagatgtagc aatgaaattt tataaaaaaa tttgggcttg tgttgaagat
    24421 ttaaatagta aagaattgat agaaaataat tcgattaaaa cttctacaaa taaaaagttt
    24481 aaaattagat atattgaaga attagattta agtataaaac aaaatgctat aactaatttt
    24541 gttattaaat acaaagataa attttataat ataactcaaa tcttaaattt agaagaagaa
    24601 aataaaatac ttgaaataac agcagaatct aagtaaaaaa aagttaggag atagtatgca
    24661 ctcaaaaata gttactatac ttaaaaaatt aaatctaggt attggctttc aagaatacga
    24721 caatttttca aatgatgaag aatatataat attttttata gaaaatgaat atgatgcaaa
    24781 tttttctgat aatgaaaata aaagtattgt atattcaata aaaatagaat attggtataa
    24841 gagcttaaag aatataaaca aatatattag tataaaagat atatttaaaa aagaaaattt
    24901 tatcttttta aattctagtg attttataga aaatgaatat tattgtaaag aattaaaatt
    24961 caaatatgag gaagttgttt aaatgggtgt agagataaca acagaaggtt ttgacgctat
    25021 tttatctaaa atagagagta tgggtaaatc gggcgataaa ctgctaaatg aagccgtaaa
    25081 agctggcggt aatgttattt tgcaagatgc attaccaagg gtcagcaaaa gaagcggaaa
    25141 gttaaaagat ggtttaaaag taagtggagt taagaaaaaa ggtggtacta agtatgtttt
    25201 agttggtatt acgaaagagg ataattcaaa aattttttat ggaaagtttt tagaatttgg
    25261 agcatccgcg catcaaatac caatcaaaaa aggtaagaaa aaaggtagag taataaatca
    25321 tccaggcgtt agtcctaaac cgtttttagc acctgcgtac gagtcaaaaa aagatgaagc
    25381 taaaaatgta atgaaagaaa tattaaaaag aggattgggg ttataatgat taatataaat
    25441 aatttagtaa tagaaacatt aaaaccttta aaaatacctg tttcatttca aaggtataat
    25501 ggagaagaaa aaacatatat aactttcttt agttatttag aacaaaattt ttatgcaaat
    25561 gatgaagtgg taggcacaga acattatata caaatagatt tgtttagtaa gaataaaatg
    25621 agttatataa ataaagaaat tgaaagactg cttaaaaaaa ataattttat aaaaagaagt
    25681 atacacgaaa taaaagaatc tgattatagt taccatactg tttttagatt cttatttttt
    25741 gcgaaaaacg aggaggatta agaaatggct attataggat taagaaacta tagaattgta
    25801 aagctactaa cagatacaga ggaaacactt gaatacgata ctaaaataac aagattaaca
    25861 ggtgctaaaa gtgtgaaaat atcacctaaa gttgactcag cagaaaatta tggagacgac
    25921 cagcttttag agactgcttc ggctatggga gcaatcgaag ttgaaatcga agttgctgat
    25981 ttgacattag aagagcgtgc gcttattctt ggataccaat ataaagatgg tgtgttgata
    26041 gaggataaaa atttcaatcc tccaaacctt gcttttggtt ttgaatcccc aaagagccaa
    26101 aacggaagta gaatggtttg gctaactaaa ggtgtctgtg aacctttcga agaagaagca
    26161 aaaacaaaag aggataaaat cgaatttcaa tctcaaaaga ttaaacttaa atttatgccg
    26221 cgcataaatg atggtagaca caaaataaca gcagatacag atgttgaaaa cgccccaaca
    26281 gatgtagagt ttttcacaac agaattttta aaaacaggag caaaaccaac tgcaccagca
    26341 aaagcaaatt taaataaata aaaaggagaa aataaaaaat ggaaataaaa ttaactataa
    26401 atgaagaaga aaaaatattt aaagctccac ctatagcact taaaaaatta gataatgcct
    26461 ttgcattatc tgataaaatt aatgatgggc ttagtatgag agggttattt caagaattat
    26521 tagattttac aatagatata tatggcgaac aatttacaaa agaagaattg ctaaatgggt
    26581 tttatcctgc tgacgaattt atgtcaaaag cattagaaga tttgtcaaaa gtatctggaa
    26641 cctttgaaga aaaagtaaaa aactaagtga tggagaaggt acagaaaata attctaataa
    26701 agaaaataag tatctatctc cacgggactt tataaaagat ttatatagta tgtttttatt
    26761 tgaaaagaat tggagcatgg tcgatataga caatatggat atattttatt atttagatat
    26821 actagcgtat atgaagaaaa ataaaagcga taagaataat ccgaatgaaa atgatgtcta
    26881 tatagaccaa ttaagctggt tataaggatg tgagtgtatg tgtaagtatt gtgaagtcga
    26941 agaaagcgaa ggagctttta aaagaggtaa aacagacaga ggggaattaa taacttgtaa
    27001 ttgtgatata gaaatgtgga ttgaaaaaga aggtaatgag tattttttaa attctgaatg
    27061 ttggtgtggt tactatagtg aaagttttga acctattaac ttttgccctt tttgtggaag
    27121 aacattaatt taataatttt gaaataaacc aagataaaat atcttggttt atttttttgc
    27181 aagtgaggtg aggaaatggc ggaagatgta ggcgaattag ttgttcgtgt tgcaatggaa
    27241 aacagctcgt ttcaacaagg gattaataac ttgaatagac aaatgaagtt aataagaagt
    27301 gagtttaaaa attctggcgc tggagtgaaa aatttcggaa ctagtcttga tggtttgaaa
    27361 tctaaacaac aaatgttatc tagtactata caacagcaaa ctaaagtagt tgaagcatat
    27421 aaaaataaac taaatgaaag taaaacaact ttaagcaaca cggcacaaaa gcaagttgaa
    27481 cttaaagaaa aagttaatca agctaagagt gcttacgaac agagcaaagc gacattaggt
    27541 gaaaacgcag aagaaacaaa aaggctaaaa tctgaacttg atagtttaga acaagaatat
    27601 gctaaaaatg aagaaaaaat aaggtctaac tcggcagcgg ttgataattg gtctattcga
    27661 gttaataatg cagagtcgag actttctgaa atgcgttcag aactagatga cacaacagaa
    27721 agaataaatc aacaagaaaa taaatggaat cagttagcaa ctaaaatgac tgaaataggt
    27781 aacaaattta gctcggttgg aaaacaaatg caagacgtag gtaaaaaaat ggctatgagt
    27841 attactgctc ctgttttagc agtcggagca gcggctagta agcttggaat ggattttgaa
    27901 gcatctatgt caaatgtaca aggattaagc ggggcaacag ctgacgaaat ggttcagctt
    27961 gaaaaagcag ctagagaagc aggagcatct acatccaaaa cagctaaaga cgcggctgat
    28021 gcgcttgggt atatggcttt ggccggctgg gatgcgaaaa catcaatgga agctttgatg
    28081 cctgttttga gattgtcgga agctggaaat ttagaccttg caagaacctc ggatttagta
    28141 actgactcta tgtcaagtct cgggcttagt gtaaaagaat tgccaacata tttagaccaa
    28201 gttgccaaaa ctgctgcaag ttcaaacact aacattgacg cacttatgga agctatgatt
    28261 gtgtctggag gtacttttaa aaatttaaac gtacctcttt cggaagctaa cgcactactt
    28321 ggaatactcg caaatagagg actaaaaggc agcgaggcag gaaattcttt aaactctatc
    28381 atgataaatc tgacaagtgg agcaggtcaa gctggtgtag ctatgaaaga actaggatta
    28441 agtgcttttg atagcaatgg taagttcaaa ggcatggcta atgttttgat ggaacttaaa
    28501 gaaaaaacaa aagacatgac agaagaacaa cgaacaatgt atttgtcaat gataggtgga
    28561 aaaacacaaa tatctacttt gcaagctttg ttatctggag ttggagagga atattcggaa
    28621 cttgaaaaaa aggttggaaa tagcacaggc gcattggata aaatggctaa gactatgcaa
    28681 aataataata aaggttctat aactgcgtta atgtcggctg tggaggagtt aggacttaaa
    28741 atatacgatg tactaaaacc aagcatagcg caagcagtta gttggtttca aaatcttaca
    28801 aataaattaa acgaaatgaa gcctagcacg gtagaaacta tagttaaaat agcaggatta
    28861 gcagcagcac tcggaccagt tctattaatt agtggaaaaa tagttagcat gataggtgga
    28921 gttatagggg cttttggtac tttttcggca gctatggcag tcgtaacgac aggagcagcc
    28981 gcagcaactc cagctgttgg tgctttagct gcaattttag gtgcattatc aagccctgcg
    29041 gctatagctg tagctggaat tactgcgcta ggcatatcgg ttgcgacagt cgctaaagac
    29101 atgtcaaagg atgctataga gccaattagc cgttttggta aaagtatatc agaatctaca
    29161 aaagaagctg tatctgcttt tatggattta gaagaaaaga caacagtatc tttaaatcaa
    29221 ttaatgtgga gtggtgagac tgtttcggag aaaatgaaac agacaatcgt atctaatttt
    29281 acagaaatgt caaatcaaat cgttgctaaa cttcaagaga gcaaagaaca aggtattcgg
    29341 tctttgcaag aaatgtttgc aacttctaaa aatttaagtg acaaagaaaa agaagagtta
    29401 ataaaaaata cagaagaagc atattcaagc aaagaagaaa aaataaaaaa gagcaatgaa
    29461 aaagtaaatc aaataatgac taaagctagt caagaaaaca gagcattaac aaaagaagaa
    29521 gcaacagaga taaatcaaat aaaaacagat atgctaaata ccgcggttga cacaatgagt
    29581 aaaagcgaag cagagcaagc ggcgataatg gaaagaatga aagcaaatca tgttgattta
    29641 tcagctaaag aagcggcgga agtagttaaa aattcaataa agaaaaaaga tgaaagtata
    29701 aaagcagcta acgaagaata taacgaaaga ttaaaaatag ctgcgcaact tagagcagag
    29761 ggtggcgcag aaaatgaaaa gttagcggat aaagtagtag aagaagcaac aagacaaaaa
    29821 gatgaatcaa taaataaagc taaagaaatg catcaaggag ttgttgcaga agcacagaaa
    29881 caagcagaaa accaagcgag taaagtcgac tggacaacag gagaaataaa atctaagtgg
    29941 caagttttaa atgatacggt aaaaggtaag atgcaagaat gggacgacat ggcgggcaac
    30001 tgggtagaag gcttgaaaga aaaaatatct actggatggg ctaatttaaa ggaaaataca
    30061 gctattttgt gggataatat aaagcttact gtaacaacta aatggacaga gataaaagag
    30121 tctatagcct ttaaaataga agaaataaaa caaagtatta gcgaaaaatg ggagtctata
    30181 aaattattct ttacaacaac gtgggaaaat ataaaaatga ttttcgggaa cgcttgggat
    30241 gctataaaac aacaagttga agaaaaattt gcgccttttg ttgaaaggtt aaaaattata
    30301 tttgatggta taaaagagtt ttttagaagc acttgggaag ttataaaaaa tatagtactt
    30361 ggtgctgtgt tattaatttt agatttagta acagggaatt ttgataaact aaaaactgac
    30421 gcaatacaaa tttgggaaaa cataaaacaa agcttatcgg atgcgtggga atctataaaa
    30481 gaagtattta acaatgcgtt gactgcaata agcgatttac tttctaacat gtgggaaggt
    30541 atgaaaagta ctgcatcaag cgtatgggaa tctataacac aaactttaag cagtatatgg
    30601 gaaaatataa agtcaacatg ttctaatgct tgggaagggc taaaaacaat ttgctcaaat
    30661 acttggacaa atataaaaac aacccttgta acctcatggg aaaacatgaa aacagctgtt
    30721 agtactaaat tgactgaaat taaaaataat attgtaactg tttggaatag tgttattact
    30781 ttttttaaaa cacttccagc caaattaaaa caagccgcta ttgatatgtt tacaagacta
    30841 aaggaaggta tagactcgaa aaaacaagat gctgttaatg catctcaaaa agtcgggact
    30901 gatgttgtaa acaaaataaa agagttccca agcaagttcc tgcaagttgg taaagatttt
    30961 gtaagcggct tagcgaatgg tatatccggc tttgcttata aagtagtaga cgaagctaaa
    31021 aaattagggc aaaaggctgc tgatgcggtt agaaaagtgt taggaataca ttctccatct
    31081 cgtgttatgg ctgagattgg taaattcgtt gatgaaggtt tagcacaagg gattgctaat
    31141 aatagcgact cggtcaaaaa agcaactgaa aagataacaa agattataga agaagaaaca
    31201 aagaaagcta caaaagcatg cgaagaagat ataaaactat ttaataaaca aatagacgaa
    31261 ttatctaaac aagaaaaagc agcgcttaaa aaagtaaaag gtgacgctaa agacgcattg
    31321 aaagaagaat atgcagctaa aaaagaaaaa ataaaagaag cgatagcact aagaaaagaa
    31381 caaaaagaaa aagaagttga acagctaaaa gagatagcaa attcagctaa agaacagtta
    31441 aataaagagt tacaagacag acaagagttt gtaaaaaaag ttgatgactt aacaaaagca
    31501 atagttgatg cgctgaaaga aaaatacaaa caagaatacg aagcacagca agcgagtata
    31561 aaaaaagagt tagacgcact agataaatgg aaggaagaaa gtatagatag aattaatagt
    31621 gtctatgatg cgaaaattaa agctattgac aacgaattag aagcttttga aaaagctgaa
    31681 aaggaaaaag atagacttga acaagacaaa gaagaattaa acaaaataga agaaattaaa
    31741 acagctataa agtttgaaca tgatgatact aacaaagaac agttgcaaaa agaactagag
    31801 aaggttttag ctgaaagaaa aaaaagaata gagcaacaag aaatagaaga taaaaaagaa
    31861 gctttaaaaa agagaaaaga agaattagaa gaaaagaaaa aagacgaaat agaaaatata
    31921 aatcaaatct atgaaagcga aaaggaatat tataataaaa gacttgaaga cgctaaaaag
    31981 ttctatgatg aaagaataaa agaatcaaaa cttcaagcag aagctgaaaa attgataatg
    32041 gataaaaatc aaaaagaaat agttgcgtta ttaaatagtt attcagatgc atataaaaat
    32101 gcaggtcaaa ctctaggtga aaaattactt gaaggattta agcctgccat aaacgaaatt
    32161 aaaaatctaa ttgatagtat tacaagagaa ataaataatg ctagaaatga agcattgagt
    32221 ttaaatagtg atattagaaa aggaaattca gcagctaatt caaattcggg aagaagagaa
    32281 gcaacttata ataataattc aagaacagcg aattataatg taaacgttaa tagtacagac
    32341 tcaaacacag caagagaaat cgaaagaaca ataagaagtc tagcatttag tttataaaaa
    32401 aatagtttat aaaaaagggg tgtgaaactt gcaaaaatta atatgtagaa actcgaaagg
    32461 gcaagaagtt aaattaggaa actcacgccc ttttttgtta gaaaaaatag aaaacggagc
    32521 aaatactagt gctaatattt cagtctcgaa caatctaggt gatggtgtaa gtatagatga
    32581 tataagcata aaagagagat tacttcctat ttcagcagct atttatgcaa aaaataaaga
    32641 agatttagaa ttaaaacgtg catatttaac ctctttgttt aatcctaaat taggagaaat
    32701 ggaatttata tatacaaata acgcattaac taaacgtgtc aaaggagttg tgcaagacat
    32761 aacatttcaa acaccagttg gattgatgca aaagtttctt atacagctct taactccaag
    32821 cccattttgg atggatgagt ttgttttaaa agaagaagtc gccttatggg ttggtgactt
    32881 cgaatttact ttagaaataa gtaatgaagg aatagaaatg ggtcataggg taagtaattt
    32941 aatttgtaat gtagtgaata acggagacgt tgaatgtggc atgaaaatac aatttaaagc
    33001 acttgcaaca gttgataatc ctagcctatt taatgtaaac acaagagaat atattaagat
    33061 aaacagaacc cttgaagctg gtgacttatt agaaataaca acagaatttg caaataaaag
    33121 aatagagtta gtaaaatcaa actatgaaag aataaatgta tttaattgga ttgacttgga
    33181 tagcgaattt ttacaacttg aagttggaga caatttattt agatatgacg cagacgtagg
    33241 aattgataat ttagaaatgt ctatatatta taatccactt tatttggggg tgtaatatgc
    33301 agcagagcat aagaatacta gataaaaata taaatttaat agctgaaata gataattacg
    33361 aagaattaca gattattaaa aaattccaca aagtaggaga gtttagttta aaaataaatg
    33421 ctaataaaaa tcatgtagat aaattagtta aaaataatat aattctactt ggtaaaaatt
    33481 ataataaagt ttgtttgata cttcatcgag agttcatgta caccgaaaca ggagaaaaaa
    33541 gcgacatttt atctgttaaa ggcgtggatt taaaaggctt gttaaataga aggctcatca
    33601 tacccgacac tggagaagct ttcgaaagtc acgaagggac acaagaagaa attataaaag
    33661 cttttgttaa taataactgc gtagaccctt caaatccaaa acgtgtaatt gataatctta
    33721 tcatttcaga aaacaaaaat cttggaagtg ctgatatgtg gcgaagttct tacgaaaatt
    33781 taagtgataa aattcaagaa atatcagaat tttgtgggct tggttgggag gtagtgttgg
    33841 atgctaataa aaagaaattt atttttgacg taattactgg taaagatttg actattaatc
    33901 aaacagaaaa cccgccggtt atttttagaa gtgattttaa taacataaga gctagacact
    33961 ttacagaaag tataattaac tctaaaaatg ttgtgtatgc tggagcaaga gaagacgcga
    34021 caaaactggt aatttcaact ggtgaggttg aaggttttga gagaaacgaa gtctttgcag
    34081 atgtaagtga aaatactata agcatactac aaaaagaagg agaaattaaa ttaaaagaat
    34141 atgaagagtt aaaaagtttt gaattagaaa tagaccctaa aaatactttt atgtatgaaa
    34201 aagactacaa actaggggat attgttacag ttcaagatag aaaattgaaa gtaacaatgg
    34261 atactagaat tgtagaagta caagaaagct atagtaaaaa tgggatgaaa ttaaaaatta
    34321 cgtttgggag cagcatacca accttatttt caaaaataaa aaggatggtg aaataatgga
    34381 aaaaagtttt gtatttaata gtgtaaatgg cgataggaga tacaaggcag aggactttag
    34441 agaatatttt gctagtttta taggtaatgg tgtattccct aatccaagta gcaatttgca
    34501 agttgtagat aataataata ataatatgac tataacagta aaaaaaggca agggctggat
    34561 taacggagct atctatataa acacagacga tttagtaatt aatatagacc ccgcagatgg
    34621 aattttaaat agaatagaca gagtagtttt aagatttgat actttaaata gaaatattaa
    34681 attagctgta aaaaaaagtg cttttaatag ttccccagct gcaacagaat tacaacgtga
    34741 tgcagatgcg tacgaacttg gattagctga tatttatatc agagctggag caataagtat
    34801 tacacaaagt tctataacag atttaagatt agataaaaat ttgtgtggaa ttgtaaaagg
    34861 tacgatagaa gaaatagaca caacaacatt acttgcacaa ctaagtactt ggaaagatga
    34921 agaaataagt aatttcaatg attggcggga aaatcaagag aataaacaag aagattggta
    34981 taacactaca acaacaaatt ttgtatcaga gtttaacatt tggtttgaaa gtataaaaga
    35041 tattttggat gaggatgcag ctggaaactt attaaatgaa ataaacaaag taaaagaaga
    35101 gttagcaaa
//
LOCUS       DAMJZE010000021        34750 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJZE010000021 DAMJZE010000000
VERSION     DAMJZE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.021"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC541_000963"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC541_000963"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2838435.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC541_000964"
     CDS             complement(2851..3216)
                     /locus_tag="PC541_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2838436.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC541_000965"
     CDS             3415..4263
                     /locus_tag="PC541_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838437.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC541_000966"
     CDS             complement(4700..5788)
                     /locus_tag="PC541_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838438.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC541_000967"
     CDS             5913..6641
                     /locus_tag="PC541_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2838439.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="PC541_000968"
     CDS             complement(6843..7334)
                     /locus_tag="PC541_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838440.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="PC541_000969"
     CDS             complement(7345..7788)
                     /locus_tag="PC541_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838441.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="PC541_000970"
     CDS             complement(7778..8617)
                     /locus_tag="PC541_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838442.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="PC541_000971"
     CDS             complement(8836..10221)
                     /locus_tag="PC541_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2838443.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="PC541_000972"
     CDS             complement(10394..11137)
                     /locus_tag="PC541_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2838444.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="PC541_000973"
     CDS             complement(11157..12419)
                     /locus_tag="PC541_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2838445.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="PC541_000974"
     CDS             complement(12603..13601)
                     /locus_tag="PC541_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2838446.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="PC541_000975"
     CDS             complement(13585..15096)
                     /locus_tag="PC541_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2838447.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="PC541_000976"
     CDS             complement(15150..16112)
                     /locus_tag="PC541_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2838448.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC541_000977"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC541_000977"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2838449.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="PC541_000978"
     CDS             complement(17092..18105)
                     /locus_tag="PC541_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2838450.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="PC541_000979"
     CDS             complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="PC541_000979"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2838451.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19517..19780)
                     /locus_tag="PC541_000980"
     CDS             complement(19517..19780)
                     /locus_tag="PC541_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838452.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19850..20923)
                     /locus_tag="PC541_000981"
     CDS             complement(19850..20923)
                     /locus_tag="PC541_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2838453.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21171..21929)
                     /locus_tag="PC541_000982"
     CDS             complement(21171..21929)
                     /locus_tag="PC541_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838454.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21926..22843)
                     /locus_tag="PC541_000983"
     CDS             complement(21926..22843)
                     /locus_tag="PC541_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838455.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22843..23043)
                     /locus_tag="PC541_000984"
     CDS             complement(22843..23043)
                     /locus_tag="PC541_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2838456.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23048..24142)
                     /locus_tag="PC541_000985"
     CDS             complement(23048..24142)
                     /locus_tag="PC541_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838457.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24652..24792)
                     /locus_tag="PC541_000986"
     CDS             complement(24652..24792)
                     /locus_tag="PC541_000986"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2838458.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(25102..26409)
                     /locus_tag="PC541_000987"
     CDS             complement(25102..26409)
                     /locus_tag="PC541_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2838459.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26474..26953)
                     /locus_tag="PC541_000988"
     CDS             complement(26474..26953)
                     /locus_tag="PC541_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2838460.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27000..27863)
                     /locus_tag="PC541_000989"
     CDS             complement(27000..27863)
                     /locus_tag="PC541_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2838461.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27863..28618)
                     /locus_tag="PC541_000990"
     CDS             complement(27863..28618)
                     /locus_tag="PC541_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2838462.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28645..29133)
                     /locus_tag="PC541_000991"
     CDS             complement(28645..29133)
                     /locus_tag="PC541_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838463.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29151..29552)
                     /locus_tag="PC541_000992"
     CDS             complement(29151..29552)
                     /locus_tag="PC541_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2838464.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29555..30052)
                     /locus_tag="PC541_000993"
     CDS             complement(29555..30052)
                     /locus_tag="PC541_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838465.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30076..30480)
                     /locus_tag="PC541_000994"
     CDS             complement(30076..30480)
                     /locus_tag="PC541_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2838466.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30710..33277)
                     /locus_tag="PC541_000995"
     CDS             complement(30710..33277)
                     /locus_tag="PC541_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2838467.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33443..34186
                     /locus_tag="PC541_000996"
     CDS             33443..34186
                     /locus_tag="PC541_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2838468.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(34498..34704)
                     /locus_tag="PC541_000997"
     CDS             complement(34498..34704)
                     /locus_tag="PC541_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838469.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT        11244 a         6368 c         3552 g        13586 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatatttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagcttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaagccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgctcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaac cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcataattca aagaaaacct agagatttct ccatgatttc
     8521 tttttaaata tttacatagt ccacaatagt aagctttgta ataatcgtat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttgtgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttattta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcttt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg taactaacat ctccaatatc caatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg tacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg ggattcttat aggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcctct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta cccagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttaata gtggatactt tttgagacaa
     9961 actatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccatagct
    10021 agccgcaaat gctgtatcca taccttctaa ttcatctata ctaaaaccat atttttttaa
    10081 gtattctttt tgtaaatttg aagcataata ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattctcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctatc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgtttag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccgaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaatcc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattaa tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 tttttcccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gtccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacctgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatctacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattcc cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaaaat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcttcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcccc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattctgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatactt ttcaacatct tctttatagg
    16201 gcataccaga ttgtgcacct agtttagtta cagaaagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tatttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattacact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg taaccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgca aaaaaaaatt aatatacttt tataattatc
    18301 taaaagtata ttaattaatt taagcagtat ttattaaagt tcaaatcctt gctctataat
    18361 cattttttta tcattttcaa tcccacttcc aacagttgtc aaataatcac caacaattgc
    18421 agaatttact cccccattaa atccaacttt gtcatatccc tttaatgata gccttccacc
    18481 agcatacctt atgtacacat taggaattat aaacctaaat actgctgttg tttttagtat
    18541 ttccattggt tctaatacat cataattttc cattggtgta ttcttaatag gatttaaaat
    18601 gtttattgga aatgatttta catttaattc tcgtatttca aaagccattt ttagtctctg
    18661 ttcttcactc tcattcattc caattatacc acctacacat acatctagcc cagctttttt
    18721 agcatctttt atagttttta tcctatcttt atatgtgtgt gttgtacata tattatgaaa
    18781 attatctcta cacgtctcaa cattgtggtg atatgtagtc acacctgctg acttcaacat
    18841 tttagcttgc tcataatcta taatacctaa agatgcacat aattttaaat tagtattttc
    18901 cctcaatcct tcatagatat ttaaaatatt attaaactcc tttcctgaca tacctttacc
    18961 acttgtaacc aatgagaatc tatgcactcc tttactctcc atctctttag ctctatttaa
    19021 gatttcattg taatctaata atgaatattc ttctacacca gttttataat gagatgattg
    19081 agcacaaaat ttacagtctt cactacattt gccagattta gcattcatta tcgtacataa
    19141 atctgctttt cttcccataa aatattctct tatttcattt gcagatttta atagaatatc
    19201 aaaatcgttt ttattatttg tatcaaggct tattaaatta agagcttctt cataacttat
    19261 ttctttttct ctcaatactt tattttttaa ttttattatg tatttttcca tatatcctcc
    19321 tatttgtcaa cttaatattt ttatgtagtt tacttattat atatagtatt gcatatataa
    19381 catttattgg tcaactttat tttagacaca agtttactat acacaaaaaa atactgcttc
    19441 aaaataatat tagtaaatgg attatacata ttattattaa aacagtattt tttattagaa
    19501 tataattctt actggtttat aaaactatcc ttgtcttaac tccatccaag ctttccattg
    19561 taataacctc taagccttca tattctctaa atagccattt taaatccttc atggcttgat
    19621 taacctgact ttttgttaga ttatttataa aatattcttt tctagtttcc tcatactctt
    19681 tgctagtcaa aaagtttctt agaaaattaa acgcaaatac acttggaata ttgtttacat
    19741 tagccttctt gctgttttcc ttctcatagt aaattttcat aaatgcatac tcctttgata
    19801 cttttagatt tctcttacaa taattatatg catttaagtg ctactattct tataatttta
    19861 ttaattcata ttctttactt cccataccca tttcacatgc atagtctatt attctagttc
    19921 catttacatg aggatgttca tgtttgaaaa catcatgacc catttcctta caaatcaaat
    19981 catagcatgc ttgttcaata gctacagggt ctgttgatgc taatattcct atatcatggg
    20041 caattggtct acctgaccaa ggtacacaat cacaaagtgg agttacattc ataacaaaag
    20101 ttatataccc taccttgtct tttttatttg atactgctcc ataagcgtat tctgccatct
    20161 tttcgacaaa cacatctgag tcactttccc attgtatagt tactgctcta gttggacaga
    20221 cagttggaca ttctccacat ccataacata catcagaatc tataactgct tttttatcca
    20281 ctatacttat agcttttgta ggacaagaat taacacactt tccacaacct acacacttct
    20341 tttcctttac tactggagta acatcagaat gttgcatttg ttttcctgct gcacttgcac
    20401 atcccatagc taagtttttt aatgctcctc caaaacctgc tgcttcatga cctttaaaat
    20461 gactcattac tatcattgca gaagaattat aaatttctcc acctattttt acactttcaa
    20521 aatgtttttt gtctattttt acattctcat aatttctact atataatcca tctgcaatta
    20581 ttactggtgc atttacaact gcataagcaa atccattttc tatagcagtt gtaagatggt
    20641 ctacagaatt tgttctactc cctgtataca gtgtatttgt atctgttaaa aatggctttc
    20701 cttcgcagtc tttaacctta tcaaccactt gtctaactgc cacaggactc atatatgtag
    20761 tatttccctt ttctccaaag tgaagtttaa ttgcaacttg gtcattcttt tctattaaat
    20821 ctttgaatcc tgcttcatca aataatcttc ttacattgtt tggtacgttt ttattttgcg
    20881 aatttgagta taaatcacaa aaatatactt ttgacttttc catatctaca cccctttaaa
    20941 atatttactt atactaattt tatcactttt aaagtaattt ttagtatatt ttaacataat
    21001 taaatattct tatatttatt tgatacaaat aatattattt gtattatttt ctacacttaa
    21061 ttctatatat taaaaatcaa aaagacttga aacttaagtc caagtctttt tgatttttag
    21121 gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta ttaaactggt
    21181 tttttattaa ataaaataac agctgctagt aaacatacta cagaaataag aactgttata
    21241 ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc tatatttttt
    21301 gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat taaactcaat
    21361 aaaagagttg ccaccaatac aagtaaaaca ctggcaaatg ctggtctaaa tagtacacac
    21421 ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa tgctgaaaat
    21481 ataatatgat taagatttgc agttggccaa aaatatattg tatagccata agcaaataaa
    21541 aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt agacaatact
    21601 atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt agaatattca
    21661 tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc agagatattg
    21721 ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt taaagtcggt
    21781 gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact tataccaaat
    21841 attagaaata aaataattaa actaaataat ttataatttc ttaaattctc tttaaattct
    21901 ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa ttttcaagtg
    21961 ataattctat tagttccatc tttactggac aaagattcat ttctgcgagt gtagatataa
    22021 ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata ttagtttctt
    22081 ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga atttcatcat
    22141 tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt tctgaaagtg
    22201 taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag attctttcaa
    22261 catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt ttaagcataa
    22321 tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca tcacaaataa
    22381 gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc attccacgag
    22441 aaaacccacc tattcgtctc ttttcatttc caagacccac aagtgataac aactcttcac
    22501 tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga gaaagatatt
    22561 caagtggtct catatagttg taaaactctg gaacatcagg cagataacct acatgacgat
    22621 tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt ccactatctg
    22681 gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca ttcttaccta
    22741 caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt atctttcgat
    22801 taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt cacctcttcc
    22861 tattgtaaaa tacaaaattg gtcctattag actaataaac agtactacaa taatccatat
    22921 tgccttattt ccaattttat aatttggatg tctcaataca tgaacaagtg cagtaattat
    22981 aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca tcaaattttc
    23041 cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat tctttgtttt
    23101 cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct gcttttacga
    23161 ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca agtttattaa
    23221 aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa tctacatatt
    23281 ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt tgtgcctgtg
    23341 ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat atatctgaaa
    23401 ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt tgcaaaatac
    23461 ccactaggtt ttggactaat gatataaggt tctgatatgc accaagttga aatgtttctc
    23521 tcgcttcatc catttgacca ttcatcattt gcccctgtgc aagaagtata gattcattta
    23581 tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga tttaacaata
    23641 tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca ttacaagttt
    23701 cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc tttttttcat
    23761 ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa ggaaaacatg
    23821 aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat ggtttaaccg
    23881 taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc atttgaggtt
    23941 cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc ggtaaaaaag
    24001 ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta gaaacgccta
    24061 tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa atcagcgttt
    24121 ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat ccttaaacat
    24181 gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca cagagaaaca
    24241 aataaacaag tatataaaat atatatttaa atttatcttt aatctctttt gtaaaatatg
    24301 taaactttgt aaataaaatc catattatat aaaactaata gttaatctat ctaaatacag
    24361 tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat aaacacctta
    24421 ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc gattttccat
    24481 ttaattcttt tataaagtta ttattcgtat ttatttctct aattataaca ggcaactatc
    24541 atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc aatcatttag
    24601 attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt attattctcc
    24661 attttcatcc caagtcataa atccccatcc actatctgga taagtatcaa tagacttttc
    24721 tctaatcata taaccaactt tataacactc ttttgttatt ttattagata gtatacatcc
    24781 ttctccattt atctaattcc attcaattaa cttttctttt caaccttaac ttttataaaa
    24841 tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat gagggtgtct
    24901 caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa tatattttca
    24961 tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat ttgatggtgt
    25021 tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc tgtaaatttg
    25081 cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca aagtatatat
    25141 ctccatattc tttcgtatat gatttattgg tactctttca tctactccat gaccaccagt
    25201 tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac ttgcatatgt
    25261 gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat aggcttctaa
    25321 taatttttta agtactaaat tactctcatc cacataatgt aagtctttat gagcaataac
    25381 ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat tgtttaaaac
    25441 tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat ttacttttaa
    25501 tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa tccctaatgc
    25561 atctccataa taatcactac aaaggtattt tgaaactaat tcgatatctt ctctatgctg
    25621 aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat tttctccctt
    25681 ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa taattccatt
    25741 aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc cccttactat
    25801 tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat gaacccttcc
    25861 tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac ttggaatttc
    25921 ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat ttgtaccaaa
    25981 aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt tcaatacatg
    26041 tataagcata gatacagcac ccttattatc tattacacct cttccataaa taatattgtc
    26101 aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa ctatatctaa
    26161 atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc caatataatt
    26221 atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt tatctaaaga
    26281 ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt gtgaatcaat
    26341 ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta cacttctgct
    26401 tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa gaacaggctt
    26461 ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca tcatgagcac
    26521 atttttgagc aaaatctaat ttaacacctt gtgcgcagtc aactcttcca taatccccat
    26581 aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat tctttccaag
    26641 cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta ttttctacta
    26701 attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc atttttcctg
    26761 gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat attgcttctt
    26821 tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga agatagaacc
    26881 atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt tctgtataca
    26941 ttttttctga catttctttc tcctcctaaa attaaattct atattttaat cactgtgaat
    27001 tagaatattc caatgaatga ccctaccaat gctaatacaa ctaaaattac taataactta
    27061 gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac accaagtggt
    27121 aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt accaccaata
    27181 tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc catcataaac
    27241 attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga tagaattttc
    27301 ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc ttcgcaaaat
    27361 agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc tgctccaaat
    27421 gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt atctccaatt
    27481 ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc tgggattttt
    27541 ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa tcctccccat
    27601 atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa atcttctttt
    27661 ctcttatata acttttctaa agccggagtg aaacttgcac aaactgctaa tgcttgcatt
    27721 ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt ttttgttaca
    27781 tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc atttttaact
    27841 tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct ttcataaagg
    27901 taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat atttcaaaat
    27961 attgaaccat aaaaaatcct ataataaaca ttggtaaaaa tttattttta cctatagtaa
    28021 atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt aatccattca
    28081 ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca aaaaagttac
    28141 atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa agtgttgcac
    28201 atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta tgaaccaata
    28261 cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct aaaggtaccg
    28321 caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt attgcaataa
    28381 gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt cctagataaa
    28441 ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa agtaatccaa
    28501 ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac cctgcaagac
    28561 ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca gattccattt
    28621 ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt catatgtcat
    28681 agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat taagttcatt
    28741 aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt tctttcctgc
    28801 ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt tataagcctt
    28861 atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta aagtgttgtt
    28921 ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc ctgatggtgc
    28981 tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat caactattat
    29041 tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat gaattaatct
    29101 gtggtctact cttactaatg ttatttttgc cattatattc acctcataat ttatattttt
    29161 ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat tctttctggt
    29221 aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat tgtctgtaag
    29281 ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc aacacttgat
    29341 gtagatcctc catataaatc tgatattata attttcacat cttctttatc tactacttct
    29401 tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa aacttcatat
    29461 tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc ccaagttcca
    29521 tggcacagta tgattacctt atatttattc attgctaaat aaactctcct ttatagcgat
    29581 taatattttg gtttaattct acttcttctt catcaggtac catttgaatt gatacattaa
    29641 tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg tatacatttt
    29701 gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt tttttgatat
    29761 taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat acaaaaacgt
    29821 tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt aatattgata
    29881 aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca tcattggttg
    29941 tttcatcatc tactactact atctttttta cacctagatg cttaacccat ttatatataa
    30001 cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac atttttaatc
    30061 aaactccttt ttctattaca ttgagctaag aactttgtta atattgttta taccagtttg
    30121 tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc tattaattaa
    30181 agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc cacctttttt
    30241 aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta atactaaaac
    30301 tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta agtcttcaat
    30361 attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg caataagttc
    30421 acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta ttcctatcac
    30481 caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc caacttatgt
    30541 gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttatt caattttttt
    30601 taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc aaatctgtat
    30661 caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat caacttttga
    30721 atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat tccatatctg
    30781 aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata actaaatata
    30841 attctttatt tctactaatt ctttcttcat atgatttata aactaaagac tcttttatta
    30901 ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt tcatcttgaa
    30961 ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata aaataagcct
    31021 tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat ctatcaaaat
    31081 atctaccacc attatctttt tcagcatatg taacttccat atctccataa tgtttcttta
    31141 tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt agagcctttc
    31201 tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact tcttttcctg
    31261 tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt aaaattaaaa
    31321 ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa atatcctgaa
    31381 ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt aaagtattgg
    31441 ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat attgctatac
    31501 tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa attgcttcat
    31561 cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct tttgctatgt
    31621 tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt actatatttc
    31681 caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt attaatttat
    31741 ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt acagtagttg
    31801 caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta tatataaaag
    31861 tattcattaa attataggaa tacttctgta tgtctccatc tataacactt ctaatttgtg
    31921 actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt ccataaaaat
    31981 ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc ttttcttctg
    32041 gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac tcctcttttc
    32101 tattatttcc atataatgat tctctaatat tatttggtag aagagagagc cttacatata
    32161 cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt aattgtatat
    32221 cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc ttaataactt
    32281 cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa aaataacata
    32341 taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt atagttacag
    32401 gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta gtggctgcaa
    32461 ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta tatatccctt
    32521 tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga acttcatcta
    32581 aaaacaatac tccattatta gcttcatgta caataccata cttatcttta tcagctccag
    32641 taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga ttatcagaat
    32701 aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt atagttaaac
    32761 tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt cctattatta
    32821 atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct atttgggatc
    32881 ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt atactctcta
    32941 aaatatactc atctttgata gaatcatttt cactatattc gtttatagag ttaatattat
    33001 caaatatccc tttatttagt ttctttccta aaatactctc aatactttct ttatataagt
    33061 actgaacagg cttacccttt atctttataa tgatactttc ttttattaat tgatttaatt
    33121 ccttactagc attacttcta tttataccta cttcagtagc tatatattct gcatcaaatc
    33181 caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc aagcaaatta
    33241 aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc tataagcagt
    33301 aatactgtta aacatttatt attaagctta attctacaat attttaatat ttttgtctaa
    33361 ttatgtaact attgattttc tttcctattc gttttataat ataaattacg ttaccctcat
    33421 cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc actctatagt
    33481 cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag aaaatggaat
    33541 taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc acagatggta
    33601 ctttcacaac attcaaaata tcccaagaat cataaataac attataattt taagaggctg
    33661 tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg taattcctcg
    33721 gttagattat cttatcttgt catttcacga agctgtgttt tctccaaata cattagagaa
    33781 taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa tattaggaca
    33841 ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag ctatagaaaa
    33901 gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa atggtagctc
    33961 ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa tcatactaac
    34021 ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa atatcttaaa
    34081 ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta ttaatcattt
    34141 tcatcaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa taatacatgc
    34201 ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata aagaaattat
    34261 aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga ttgatatggt
    34321 ctatgatagc aatagaaatt ataacatttt taataagctt agttttatat accttatagt
    34381 ataaagctaa aaaaaagaat catctaaaat aaagtaaatc ttatataagg tttactttat
    34441 ttttatttgt aataatcatc aatacaaatt attttattat atgtaattat ttatgtctta
    34501 accaatttta tcgaaaataa cagtcttctc ttttaaagag atttttccta cttgatatcc
    34561 atattcattt aattcctttt tatatttcaa aaacgaatgg tctatctcta tcccagcaat
    34621 atcttttatt tcttcaaaag ttaatttaaa cgattgacta ttgtttttct taacatattc
    34681 ccataaagaa ctatatttac tcatcttata tcactccttt taaaatcata tcttatctat
    34741 taatttatat
//
LOCUS       DAMJZE010000022        34682 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJZE010000022 DAMJZE010000000
VERSION     DAMJZE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.022"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(68..724)
                     /locus_tag="PC541_000998"
     CDS             complement(68..724)
                     /locus_tag="PC541_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2838470.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(779..1408)
                     /locus_tag="PC541_000999"
     CDS             complement(779..1408)
                     /locus_tag="PC541_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2838471.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1539..2180)
                     /locus_tag="PC541_001000"
     CDS             complement(1539..2180)
                     /locus_tag="PC541_001000"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2838472.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2189..2860)
                     /locus_tag="PC541_001001"
     CDS             complement(2189..2860)
                     /locus_tag="PC541_001001"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2838473.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2865..3749)
                     /gene="rsgA"
                     /locus_tag="PC541_001002"
     CDS             complement(2865..3749)
                     /gene="rsgA"
                     /locus_tag="PC541_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2838474.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3847..4584)
                     /locus_tag="PC541_001003"
     CDS             complement(3847..4584)
                     /locus_tag="PC541_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2838475.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4692..6707)
                     /gene="pknB"
                     /locus_tag="PC541_001004"
     CDS             complement(4692..6707)
                     /gene="pknB"
                     /locus_tag="PC541_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2838476.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6724..7476)
                     /locus_tag="PC541_001005"
     CDS             complement(6724..7476)
                     /locus_tag="PC541_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2838477.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7496..8527)
                     /gene="rlmN"
                     /locus_tag="PC541_001006"
     CDS             complement(7496..8527)
                     /gene="rlmN"
                     /locus_tag="PC541_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2838478.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8535..9860)
                     /gene="rsmB"
                     /locus_tag="PC541_001007"
     CDS             complement(8535..9860)
                     /gene="rsmB"
                     /locus_tag="PC541_001007"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2838479.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(9922..10623)
                     /locus_tag="PC541_001008"
     CDS             complement(9922..10623)
                     /locus_tag="PC541_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2838480.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10692..11444)
                     /locus_tag="PC541_001009"
     CDS             complement(10692..11444)
                     /locus_tag="PC541_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2838481.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(11454..12383)
                     /locus_tag="PC541_001010"
     CDS             complement(11454..12383)
                     /locus_tag="PC541_001010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2838482.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12398..12838)
                     /gene="def"
                     /locus_tag="PC541_001011"
     CDS             complement(12398..12838)
                     /gene="def"
                     /locus_tag="PC541_001011"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2838483.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12856..15381)
                     /gene="priA"
                     /locus_tag="PC541_001012"
     CDS             complement(12856..15381)
                     /gene="priA"
                     /locus_tag="PC541_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2838484.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15620..16819)
                     /gene="coaBC"
                     /locus_tag="PC541_001013"
     CDS             complement(15620..16819)
                     /gene="coaBC"
                     /locus_tag="PC541_001013"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2838485.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16824..17090)
                     /locus_tag="PC541_001014"
     CDS             complement(16824..17090)
                     /locus_tag="PC541_001014"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2838486.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17093..17710)
                     /gene="gmk"
                     /locus_tag="PC541_001015"
     CDS             complement(17093..17710)
                     /gene="gmk"
                     /locus_tag="PC541_001015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2838487.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17740..18621)
                     /locus_tag="PC541_001016"
     CDS             complement(17740..18621)
                     /locus_tag="PC541_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2838488.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18625..19455)
                     /locus_tag="PC541_001017"
     CDS             complement(18625..19455)
                     /locus_tag="PC541_001017"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2838489.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19742..20776)
                     /gene="ltaE"
                     /locus_tag="PC541_001018"
     CDS             complement(19742..20776)
                     /gene="ltaE"
                     /locus_tag="PC541_001018"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2838490.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21072..22847
                     /locus_tag="PC541_001019"
     CDS             21072..22847
                     /locus_tag="PC541_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2838491.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23062..24714)
                     /gene="abc-f"
                     /locus_tag="PC541_001020"
     CDS             complement(23062..24714)
                     /gene="abc-f"
                     /locus_tag="PC541_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDF2838492.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25013..26224)
                     /locus_tag="PC541_001021"
     CDS             complement(25013..26224)
                     /locus_tag="PC541_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2838493.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26508..27041)
                     /gene="pyrR"
                     /locus_tag="PC541_001022"
     CDS             complement(26508..27041)
                     /gene="pyrR"
                     /locus_tag="PC541_001022"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2838494.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27401..28348)
                     /locus_tag="PC541_001023"
     CDS             complement(27401..28348)
                     /locus_tag="PC541_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2838495.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28317..28763)
                     /gene="lspA"
                     /locus_tag="PC541_001024"
     CDS             complement(28317..28763)
                     /gene="lspA"
                     /locus_tag="PC541_001024"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2838496.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29073..29852)
                     /locus_tag="PC541_001025"
     CDS             complement(29073..29852)
                     /locus_tag="PC541_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2838497.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(29907..30389)
                     /locus_tag="PC541_001026"
     CDS             complement(29907..30389)
                     /locus_tag="PC541_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2838498.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30655..32076)
                     /locus_tag="PC541_001027"
     CDS             complement(30655..32076)
                     /locus_tag="PC541_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2838499.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(32222..32992)
                     /locus_tag="PC541_001028"
                     /pseudo
     CDS             complement(32222..32992)
                     /locus_tag="PC541_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(32997..34199)
                     /locus_tag="PC541_001029"
     CDS             complement(32997..34199)
                     /locus_tag="PC541_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2838500.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11033 a         6375 c         3481 g        13793 t
ORIGIN      
        1 tttttaaata tttattttta atttattttg tttttaattt acttcgattt taaaatgtcc
       61 ttgactttta ttttataaat gtacatattt cttctactgg ttttttagat ggactcttca
      121 aattgtcctc tggcactcca agagctaaca ttgcacctaa aaaatactct cccttttcaa
      181 aaccagtttc tgcttctaaa acagcttcta tctcatctgc tgcatagttt tgacttgtca
      241 accaacaaga accatatcca agttcaattg ctgatagtgt aaagttttca attgcagcac
      301 ctagactctg catacctgga tttcttataa ataatttatc tatagtctcc ttaggtgcat
      361 ctataagctc taattcataa tatcctgatg gattatatac ctttgtaaaa actaatacta
      421 aaactggagc ttttaagtaa aatagtgtaa aatttttaac aaattttcta aatctattag
      481 ccttatccac tgatacttta tccatttcta caagtatttc ttgttgcttt ttagttatca
      541 catctgctat cttttccatt aaatcacggc gttttataac aacaaaatgc cagttttgaa
      601 tattcttgcc tgatggtgct gcacctgctg cttttatcat ttttaaaata tcttcatctg
      661 atacatcttg atttttaaac tttcttacac tttgtctttt aaaaatagta tcttgtaatt
      721 ccatttttaa atccccccat aactttttta ttattttaat ataattttta aactcatctt
      781 attttaatat atcatgttct aacaaatctt taaatgaatc tataaaataa tcatatacat
      841 ctacattccc aaacccatat ttggcatata taaaaggtat acctacaaac ttagcactat
      901 ttgcatcacc ttgcgtatca ccaacataga ttggatttat taatttattt ctttcaatta
      961 taagtttatt attttctcct ttatgcaaac cagtattacc aggacattca aaatctataa
     1021 aatactgttc tagtttatgt gcttttaaaa aagattctat atatccagct tgacaattac
     1081 ttactataaa taatttatat tttttagata gtaatttaag agtatcttct atatcatcaa
     1141 ataatttagc tccattttca gataaatagt cacattcttt caaagaacat tcattcaata
     1201 aattcattct ttcttcttca cttaaatttg gaaataaacg atttgctatt tcctcaaaag
     1261 aaagccccat aagacttcta aataaatctt ttgtgacact taattctatt tccttatgct
     1321 cacttaaaac atcctgccag actttgcata cttcttcagt tgaatcccac aatgttccat
     1381 ctaaatcaaa aataatacta tctaccacaa aaaatccccc ttatgtcaaa gtttattata
     1441 tatttataat tatatactaa aaagcagata caatcataaa tatattttac ttgattgtat
     1501 ctgctctaca atataaaacc aattgtcaaa atcaaatttt ataaatttct aactacaagt
     1561 acacagcctt gctctacttt aatcttacaa gaattactca acataacatt tgatattcca
     1621 ataggtacag agtatttcat ataataatta tcgagagggt attctaagcc agtaagtgtt
     1681 acacccttag catcaccatt tatagctatt acagaaatag tgtcccctgg gtttccatac
     1741 aatgaaattt cctcattttc aactatatac atctcttctt tatcagaaag tatcctagaa
     1801 tatataccat cttcttttaa ataatataat agctttatat ttgcaagagt gtggtcaatc
     1861 cttcccccta aagcaccaaa aaaatcaata tgatttgctt taagtgtctt agctaaaaaa
     1921 agacaaagct ctgtatcagt ctcatctttt ttagatggaa atttttcaaa tttaacacct
     1981 ttatttttat agaagtttat cttttcttct tctactgaat ctaaatctcc taatatataa
     2041 tctggcatta tttccatttt ataagtgtga tttgcccctc catctgcaca tattatgcag
     2101 tcataacatt ccttaattat aatatctctt gtaacttcgt aatcttcaat ttcgccattt
     2161 aaaacaatac agattttcat tattaaattt aaactaattc ttcaagagaa gcattctttc
     2221 taaataaatt aactgtttct tgaatgtcat cacttccaaa tattgcagaa cctgctacta
     2281 ttacattagc tcctgcttgt acaacttcgg ctacgttttt agggcttata ccaccatcaa
     2341 cttctatatc aatgtttaat ccttgactat ctataatagc ttttaactct ttaatcttat
     2401 ctaaaacaca aggaataaat gattgtcctc caaatccagg gttaacagac attaataata
     2461 ccatatctaa atcaggtagt acatgtttaa tcgtatctac tggtgtagct ggattaagta
     2521 ctacaccagc ttttattcca tgtgatttta tattttgtat agttctatgt agatgaacac
     2581 aagcctcctg gtgaacagtt attatatcac atcctgcttt tacaaattct tcaatatact
     2641 ggtctggatt ttctatcata agatgagcat caaataccat atttatattt tctttcttta
     2701 aacttttgac tacaagtggt cctaatgtaa tgtttggtac aaagtgtcca tccattacat
     2761 cgatatgaag atattcacat cctgcacttt ctacttttct tacatcttct aataattttg
     2821 caaaatctgc tgataatatt gatggtgcta atttaatcat gtttctaata cctcctacta
     2881 ttattttgtc ttatttcatg tagcagctgt atataactat catatctttc tttagatatt
     2941 tctccatttg taacagcttc ttttatagca caattaggtt cattttcatg aatacacttt
     3001 gaaccaaact tacaatcatt aaatttatca aattctataa aataatcttt aagttcaact
     3061 tcctctatat cttcaagggc aagggaacta aaacctggtg tatctgcaac cattccacca
     3121 aattctaatt ttaaaagttc agcgtgacga gtagtatgtt ttcccctttt tattttatca
     3181 cttacaactc ctgtttgaag tttaaaattt tcatctattt catttaataa gcttgatttt
     3241 ccaactccag atggtcctgc aaacactact acattttctt ttaattcttc ttttacttta
     3301 tctatattta acttagtaat attgcttaca ggaatgactt tatagccact aagttcatat
     3361 atatttttaa cagtttctaa tgtatcatta tcatctaaat ctgcttttgt aaggattatt
     3421 acagtttcta aattttcttt ttctgccaaa actataaatc tatctaaaag agataagttt
     3481 ggtttaggat tttttattgc gaatactatt aatgctttat ctacatttgc aataggaggt
     3541 cttataagtt ctgtatctct actgtcgatt tcttctaaga tacccttttt ttcattttca
     3601 tcaacaatgc ttattttgac tctatcacca accaaaggtg ttattttctg ctttctaaat
     3661 atccctctag ccttacattc ataaatacca ttataagtat ctacataata aaatccacta
     3721 atccccttta taatttttcc ttctagcatc aaatgcctcc ctttataaaa tttatataaa
     3781 cacatactgt ttatgagtag tatatcgtgt tattctttcc tatttttata taataccaca
     3841 ttttttttaa ataacaaatc ctccgtttgg actaattacc tgaccagtga taaaatttga
     3901 tttgtctgat gccaagaaaa tagcacaatt tgctatatct tctggagttc ctagcctcat
     3961 tagaggggtt tcttccacaa gagcgtctat atcttcctca ttatatccag ataacatgtc
     4021 agtattaatg acacctggag ctatgctatt tactctaata tttgatggtc ctacttcttt
     4081 agctaatgcc tttgtcatac ctattatgcc tgcttttgtt atagaataat gcacttcaca
     4141 agacgcacct gatatacccc agattgaaga tatattaatt atatttcctt ttttttctga
     4201 tatcatgtat tttaaagcaa cttgtgaaca ataaaagctt ccttttaaat taatattcat
     4261 catattatcc caatcttcat cagttatatc tgtaaacaac ttatcttgac ttacaccagc
     4321 attgtttaca agtacatcca atcctccaaa ttcttttata caataatcta ccatatcctc
     4381 tacatcttct ctatttgaaa tatcagcctt aaaaagtttt acagaaaaat ttttttcatt
     4441 taatatagtg tacaactctt tagcttcatt ttctgactta ttaaaattta ctaatacatt
     4501 gtaaccttct tttgcaaaag ctttagacat agctttacca atccctctag ctcctcctgt
     4561 aattaaaact gttttcttct tcataaatac ttttctcctt atattataac caattcagta
     4621 ttttatacaa aacaaactgc cctttaaaga aaatattttc tataaaagga cagctcttat
     4681 tttattaata tttattctgt tttaccaatg tttgcgccag ttcctgatga agttcctcca
     4741 ttatctcctg agtttgagcc tcctgaacta tctccattgt ctcctgagtt tgaacctcct
     4801 gaacttcctc cattgtctcc tgagtttgag cctcctgaac ttcctccatt atctcctgag
     4861 tttgagcctc ctgagtttcc accactattt cctgagtttg aacctcctga gttacttcca
     4921 ttattgcctg agtttccacc actatttcct gaatttgagc ctcctgaatt tccaccactg
     4981 tttcctgagt ttgaaccagg ttctgttggt gtattattct ctggtgttgt tggtttttta
     5041 tctggtactg ttggagtctg tgtatttgat ttttctgaac ctttgcttac atataggttt
     5101 acctcatcac cttcctgagc tcttgatgag ccagcacccg gactctgact taaaactgta
     5161 ccttctttat atattgagct gtattcatac ttaacagttc ccacttttaa gttattttct
     5221 tcaataagtt gttttgcatt actcaaagta agtcccacta cattaggaac agatgcctga
     5281 cttgaacctt tacttacgac aacatttata gtatcaccct ttttgacatt tgtacttcca
     5341 ccttcaggtg tctgtgatat tatacaattt tcatcaactt cactattata ctcttcactt
     5401 tttacactca ggtataatcc ttctttttca acagcacttt gagcttcttc taatgtcata
     5461 tttacaagat taggaacagt caaagattta ttgccaaacc ctccagcaaa taaaaactta
     5521 tatgctaaga atacttgagc acacaatatc agtatcaaaa cagccgcaat tacttttaat
     5581 cttcttctag attttgggct ttcctgtttt ttcttctttt tcttagttct cttacttgga
     5641 gtactcttag gtctctgatt ctttttagct ctcattatct cttcctcttc ttcatcttca
     5701 tcatcatctt cttcataaaa atcatcatag taatcttcat catcatccaa atcagctact
     5761 tcaacaggct taaccacttt ttcaggagct ggttttgcaa gtgttggatt tacaacttta
     5821 ttaatttctt tttcatcaat tttcttagtt gcaaaatcat catattcttt tataaaatct
     5881 aaatcaatat ttttttctat atactctata tcttctatca attcttcagc agtttgatat
     5941 ctatctgcac tagatttttc tgtaagcttt tttattgtag ttcttacact ttgaggaatc
     6001 ctaacttttt cttctgatgt aaagtctata tcatcattaa tgtgttgaag tgctatagaa
     6061 attggactat ctcccctaaa tggaactttt cctatcaaca tttcatataa aactatacct
     6121 agagaataca ggtcagcatt attagttaca aatttacctt tagcttgctc tggtgaaaaa
     6181 taatgcactg aacctattat actaccaata ttagtcattg ttgaatttga aacagctttg
     6241 gctataccaa aatcagccac ttttactact cttccttcat ttgatatgag aatattatga
     6301 ggttttatat ctctatgtat aatccccttt ttgtgcgctg cactaagtgc catagctatt
     6361 tgttttgtta tatctaatgc agtatattca tctaatatac cttcatcttg tataatctct
     6421 tttagattct tcccatctac aaactccatt acaatgtagt gaacttttcc atcttcacca
     6481 acatcataaa catttactat atttggatgt gaaagacttg ctactgcttc tgcctctctt
     6541 ttaaactttg ttagaaattc atcatcatct acaaattcag gtctaagtac tttaagtgca
     6601 acagtcctgt ttaataatct gtctttagcc tcatatacaa aggccattcc tccatcacca
     6661 atcttcctga tgatttcata tcgatttcct aaaattgtat ctcccacaat atcaccgtcc
     6721 taattatact tttatcacta taaccgaaac attatcttta ccagaaacat cattggccat
     6781 acttatcaat tcttcactta tattactagt tctctcatat tcataatcta atattaaatc
     6841 tcttatatct tcatcatcaa taaacccagt taatccatct gtcgcaagaa gtattatatc
     6901 actttttttg atttctttct taaaaatatc tacaacaacc atatcatctg ttccaattgc
     6961 tctagttatt ctattcctct gtggatgtct tcttgcctct tcttctgtaa tgacatgggc
     7021 catcataagt tcttcaacca cggaatggtc tatagttatt ttatcaaatt tttcatctgt
     7081 taacaaataa catctgctat ctccaacatt tgccacatat aagacattat tatacactat
     7141 agccgtaact aatgtagttc ccattccttc aaattctata tcttccatag attttttgtg
     7201 cacaattgag tttacattat tataagcctg ttttaatata tcatctatat agtctatttt
     7261 tacattatca tgttgtaata agttttcttt tagaaaatca attatatttt ctactgctaa
     7321 tttactggcg acttcacctt ttttatgtcc acccattcca tcagctatgg caaatattcc
     7381 tattggacca tgttctgtat ctataacttc tccctcacaa tagtcttcat tgttttttct
     7441 tatttttcct atatgggagg cacaactata aaccatacga cttcctcctt acaaattact
     7501 tgtattttct tctcaattgc ccacaagctc cactaatatc agaacccata gacatcctaa
     7561 ctgtggcagg tatattgttc ttctccaaac tatccctaaa tttataaata aatgctttat
     7621 ctggtttttc gtattctctt tcttcaactt tatttattgg aattaaattt acatgacata
     7681 gcataccttt taggagctta gctaatgcct ttgcttcttt ttctgagtca tttactcctt
     7741 ttataagaga atattcaaat gtaacccttc tatttgtctt ttttatataa tatctacatg
     7801 cattcaatat ttcttcaata gaataagcat ttgctacagg cataattttt cttcgttctt
     7861 catcatatgg tgaatgaagt gataaagcta aatttatagc tatttctaag tctgccaact
     7921 catacatttt aggtactatt ccacaagttg aaagagttat atgtctatac cctatattaa
     7981 gaccattttt ttcgttaatt atttttaaaa attgttttgt attttcaaag ttatcaagtg
     8041 gctcaccact ccccatcaat acaagatttg ataccctctt accagtatct tcttgaattt
     8101 taataacttg gtctaaaatt tcccatggct ctaagtttct aattaatcca tccattgtag
     8161 atgcacaaaa attacatccc atcctacaac ctacttggtt agaaacacag acagtaactc
     8221 tactatcata atccatcatc acagtttcta ttatatttcc atcatctaat aaaaacaaat
     8281 atttttttgt attatctacc ttagattcta actttaattc tatatctata tgccctatac
     8341 atgaaacctc ttctaattta tttctcaaac tttttggtat attgttcata tcatcaaaag
     8401 tttttgcacc cttatatatc caagagaata tttgacttcc tctaaagggc ttttcatcta
     8461 tagttttcat aaactcttta agttcatctt cagtaaaatt ttttaatact atctttttct
     8521 tttccattct ctcactacct tattctcttt aattttgcta tgaaaaaacc atctatacca
     8581 tgaatattag gatatatctt taaatatcct ttatcttgat tgtctaagtc tacatttact
     8641 gtatcaattg gaacaaattc aaagttgtga ttttcttcaa caaaagaagt aactatattt
     8701 atattctcca catcttgaat tgtacatgta ctatatacta aagttccacc aactttgaca
     8761 tatttagatg cattttctaa aattttcttt tgtatagaag tgatgtcttt taactcttct
     8821 tctttcttat acttaatttc tggtttacgt cttattatac caaaaccaga gcaaggaaca
     8881 tctgctaaaa cataatcaaa tttacttata ctattttcat caatctcaga agcatcaaat
     8941 ctttctacaa acacattttt aagccccaat ctatttacag tagagtttat aagctttagt
     9001 ttatgctcaa aaatatctct agctactact tgaccagtat tgttcattaa agtagctaag
     9061 tgagtagatt tccctccagg agcactacat acatccaaaa caaatgaacc ttcttttgga
     9121 ttaattactt tccctactat catagaactt atatcttgaa ttgtaaataa accagcctta
     9181 aataattcat ttttttctat atttttcatt ttttcgactt taatagcttc ttctaccata
     9241 ggaactttta aacatacaat acccatttca cttaattttt caataagttc ttctctactt
     9301 attttaagtg tattcgttct tatatagata ctaggctttt cattatttgc ttccaacaaa
     9361 tcttcagcaa attcttgtcc aaatttatct atccagtttt ttataatcca agagttatag
     9421 gaatacttta tagataagta ttttacacta tcttcctcag ttatgtccat tatagtctct
     9481 ttagctctta tttcatttct aagaatagca tttatgaaac cagaagattt tttatcatat
     9541 ttcttagata attttacagt ttcattaact gctgcataat ctgatatact atctaaaaat
     9601 aaaatctgat atgttcccat tcttaaaaag attttaacat aagttgacat tttttttacc
     9661 tttatttttg aaagtttatt gattatataa tctaaataat atttattttc tattactcca
     9721 taaattaact ctgttgcaag tcctctatcc atatcactta tttctaaatt tttaaaatgc
     9781 ttatttattg caatatttga ataattgtca tttttttcta tatcacataa tactttaaat
     9841 ccaacttctc ttgcatccat ataaatttct cccttttatt aaacataaaa aagggttgtt
     9901 tctaaacaac ccactttact tttagtcatt tcttctttga gctattagta acaatctaag
     9961 taattgaagt attgaaacca atgctgctgc aacatatgtt aaagctgctg ctgaaagtac
    10021 tcttctactt tgtcttgact cactttcatc tataatccct aaattcccaa gttgaacgat
    10081 tgctctactt gaagcattaa attctacagg caatgttatt atttggaata acactgaagc
    10141 cgaaaacaat agtataccaa tttttaaaaa tggaccagcc ataataaatc ctaaaaatat
    10201 taaaaaccaa gatatacttg aagcaaaatt cactactggt actaaactac ttcttatttg
    10261 aagaggtgca taacctttag catgttgtat ggcatgacca cactcatgtg cagctactgc
    10321 aacagaagtt atagatgttc cataatatac atcttctgac aaccttacag cctttcttct
    10381 tgggtcataa tggtcactta gatgccctct aaccatttct atctttacat catacaaacc
    10441 atttgaatca agtactcttc ttgctgtttg ctctccagta tatcctcgac gtgtatttac
    10501 tcttaaatac ttatttgttg ttgaactcac tttaaattgt gcatatatag taagtaatat
    10561 agctggaatt aatacaacca ttgttgggtc aaatccccaa aaaccgccat atacagggta
    10621 cataaataac actctcctaa aatatatttt tattatacat cctcttaata ttcacttcac
    10681 ttaaaaacag tttaattaca aactaatata ttttttcctg ttaaaaagcc tatagctttt
    10741 tcaatatcca taatgtctac attagtattt acacaaggtc cattaggtct tttgttaaat
    10801 acacctaaaa caggaatttt cttaatatct tgtattccag atgtcaaatc tctttcacat
    10861 gcaacagcta caacagcttt aggtttatta tccattataa tttttctagc tagggttcct
    10921 ccagtagcta caaatatttt tacattagtt ttttccttta gcttaattaa ttctcctatg
    10981 ttgcatcttc cacattcttt acaattatct atatcattag taacttttaa tttacaacta
    11041 ttctcttgaa tacaatgagg tattaaaatt attatatcct ctggattaaa attatattta
    11101 ttactgtata tatattcatt atttaacttt atatatattc ttctaatatc actttttgac
    11161 ataccaaatg atgatgcaat gaaagatata ataggaaata atccactgac aatattaaaa
    11221 tttattttca ttatatttga gttaacatac tttcctttaa ttactctata agtaactgct
    11281 gttgaacata gtattataag tattagtgat attattataa tatttatcat caaggagaat
    11341 atttgagtta aacttattat aaaaaaatta acaactccta tcaaaattcc taatattaaa
    11401 atcaaaatcc caacacttgt taaatacttc tttatatctg tcatactttt cacctaaccc
    11461 aatactaaac cagtttctat agtattccct tttatatatt caccaaccaa cattctcttt
    11521 ttattcggca tctgaatttc ttcaattaaa agtacattat ctatagtact tacttttatc
    11581 ccatctttgc ttacatcaat tattgtacct acatcttttg tgcttttttc atctaacact
    11641 ttagttttcc aaactttcat tatgacatca ttataggtag tataggcact tggccatgga
    11701 tttactcctc taactaaatt gtgtatttct ctggcacttt ttgaaaaatc tatgttacct
    11761 aatgatttat tcattattgg cgcataggaa aattcttcat gattttgtac ttctcttgga
    11821 gcatttcctt cttcaattaa tcttaatgtt tcttttaaag tttctgcgcc tatattcatc
    11881 attttatcat gaagttctcc tgctgttata ttttcatcaa gatttacttc tgtctttagt
    11941 atcatatctc cagtatctag cccttcatcc atatacatag ttgtaacacc agtcttttct
    12001 tcaccattta ttattaccca atttataggt gccgcacctc tatattttgg aagtaaagaa
    12061 acatgaacat ttatacatcc aagtttagga atctctaata tttcttttgg aagtatctgt
    12121 ccaaaagcta caactactat tacatctgga tttaaagatt taattttatc tataaattct
    12181 ttatctctag cctttactgg ttgatataca ggtatattat tttcaattgc cagttctttt
    12241 actggactca tacctagttt tttacctctt ccttttggtt tatctggttg agttacaaca
    12301 cccaatattt catacttctc atctattatt ttttgtaaac atggaactgc tatatcagga
    12361 gttcccataa atactatttt catcaaatca actcctacta cttttctatt ttgtcaacaa
    12421 acaaaattcc atctaagtgg tctatctcat gacaaaatgc tctagctaaa agttcttctc
    12481 cctctaactc tattgtttca ccatttctat ttaaagctct gacctttaca taatttggtc
    12541 ttctaacatt tcccgcttct ccaacaacac ttaaacatcc ttcctcatct atttgctccc
    12601 cagatgtttc tattatttca ggatttataa gttctataag ctcttctcca atatctatta
    12661 ctactactct ttttaatatt ccaacttgag gtgcagccag tccaacacca tctgcatcat
    12721 acatagtttc tgccatatca tctaataatt gtattatctt ttcatcaatt ttttcaacct
    12781 tttttgattt ttttcttaat actggttctc ctatttgaac tatttgtctt aaagccatta
    12841 taattcctcc taaatttata atacactgtt tggattaata tctatagata cattgatatt
    12901 cttatcaaat atcaaatctc gttttgttat acatatatat tttattatac cctttaagag
    12961 attaatttca atattttcat ctttaaagag tatttgccat ctataatttt gatttatttt
    13021 tgatattgaa catggatttg gacctaatat aaatcctaaa tcatttatac cccttttttc
    13081 taataaataa atcaatgatg catgcatatt ttttatattt tttatcacta gtctttcatc
    13141 ttcaccacta acaactacac ttaacatatt attaaaagga gaatatccaa atgcttttct
    13201 tatttttatc tcatcttcat aaaaaccctc ataatcatat tcaatagcat gcttaatagc
    13261 atagtgttca gtgtcataag tctgaagtac tacttttccc tctttatcag ctctccctgc
    13321 tcttccagag acttgagtta tcaattgaaa tgtagtttca aaacttttaa agtctggaaa
    13381 atttaatatc atgtctgcag acaaaatacc tactaaagtt acattctcaa aatccagccc
    13441 tttacttaac atctgtgttc caataagtac atctgcttct ttatctttaa atttatttag
    13501 tatctcatct aaagcacctt tttttgaagt tgtatcttta tccattctca aagtttttat
    13561 atctggaaaa atatatttta attcctcttc aattttttga gttccaactc caaatggctt
    13621 aacataagta cttttacatt caggacactc ttttggaatt tctttctcat atccacaata
    13681 gtggcatctt ccagtattgc ttttcttatg ataagttaaa gatatatcac aattttcaca
    13741 ttgaaacaca tatccacact ttctacaaga tacaaaattt gcataacctc ttctgtttaa
    13801 aaatagtatt acttgattat tttcttcaat cgcataacgt atttcttttt gtaatttcaa
    13861 actaaatata cttctatttc ctttatctaa ctcatctttc atgtctacca cttcaatgtt
    13921 tggaagaggc ttatcatttg ctctactttt tatatttata agtttgtatt ctccagattt
    13981 tgccctataa tactcttcaa ttgagggcgt tgctgaacct aaaataagag ttatattatt
    14041 ttttaatgcc ataaatctag caacttctat agcattaaat tttggattct tctcagactt
    14101 atatgatgac tcatgacatt catcaattat tattaagcct aaggaattaa aaggtgcaaa
    14161 taaagctgac ctagctccaa ttaacactct tactttacct gctttgacag ctttgtaaac
    14221 atcatgtttt tctccttcag atagtttact gtggaacact ccaaccaaat ctccaaacct
    14281 actctttagc ctatctattg tttgaggagt taatgctatc tcaggtacta agaatatact
    14341 gtctaagcct tgatttagag caaattctat tatttccatg taaacttcag tttttccact
    14401 tccagttact ccatgaatta agtatggttt tttatcatct acaaacatat tcgactttat
    14461 ctcatcaact gcttcttgtt gctcattgtt taattttata ctttttagta cggatttgta
    14521 aatacttttt ggctctctat aataatcctt aaattctagg gttattagct ttttcttact
    14581 tagagaattt atactttgtt ttgaagcatt taataaatcc aataaatcat ttatctctac
    14641 attttcatta tttttaagaa aacttaatat ctctttttgt ttacttccca catttatttt
    14701 attttgctct atataatcgt caatcttatc actttctaaa ctcaaagata tataacatac
    14761 cttcttttca tttttgtgat ttttatattc ccaacataat tttataattc cattcttatt
    14821 cattgtgtac aaagaactat ttaagtttgg tatatatttt aacttatcaa ccttaatttt
    14881 acctttacta tcaatcactt tctctactat ttctctattt ttatcactta aagttgatat
    14941 tttttccttc aattcaatat catttaatcc acttaaactt tcacctaata ggactacttt
    15001 ataattattt aatttgtatc cctttggata tatcaaattt atacattcta tataagtaca
    15061 caaatatcta tttttcatcc aataaaccaa ctctaaatct tcttttctaa atatcggatt
    15121 ttcatctaat atatcaataa tttcttttat ttttattttt tcatctaagc tatctgtaaa
    15181 ttgaaataca aaggcttctg ttggtttatt accttttcca aatggtacta atattctgtg
    15241 cccaattttt attacatcag ataaaaatac aggaatttga tatgtaaata aattatctgt
    15301 atatatagta ttacttctaa ctattacctt tgcatatttt ttcattataa aacctctact
    15361 taaatttaat atattgttca tattttaata tactttacaa gatattaaaa tctcagaatt
    15421 tataagcaat ttcaagttat taatattaac tttattttaa taattaacta ttaattatat
    15481 tttatacttt atgacaaaat aaatgctgaa aactacaatt taactgacat ttgcgagttt
    15541 ttactcgcta aaattaaatt gcagctttca gccactttag ctgtgataaa atacacaaat
    15601 atcatattta aaaaaggtct tatttctcaa gcaacgcttt aactttatct aaaataacat
    15661 tggctacttc ttcttttttc atcttaggat actcagtaat atttccctct acatctatta
    15721 tttttacaat attagtatca gctctaaaac cagcaccttc tttagttaaa tcatttgcca
    15781 caataaaatc taaatttttc ttttttattt ttcctttggc atgttctata aggtcattag
    15841 tttcagctgc aaatccaatt agtatcttat cttcttttat ttttcctatt tcataagcaa
    15901 tatcataatt tctacttagt tctattgata aatcatcatt cttcttttta atttttttgt
    15961 cagaataagt ttttggttta taatctgcaa ctgctgcact ttgaattact attttatttt
    16021 cacttaaatt tgatactact gcttcataca aatcttgtgc tgactctatt tgaacaaatt
    16081 ttttaagatt ttgtggaata gaaagatttg taggacctga tattaatgtt acatcagcac
    16141 ctctagctat agcttctctt gctatagaat atcccatttt accagaagac ctattagtta
    16201 aatatcttac tgggtctata ctctctacag ttggaccagc tgtaactatc atactttgac
    16261 cttttaaatc ttgctcttta gtaagattag tcaaaacttc ctctactatt atatctggat
    16321 tagctagttt acctgttcca atatctccac atgcaagcct tccactttcc ggttcaataa
    16381 atttatatcc aaagctcttt aaagtttgta tatttttttg aacaattgga ttttcataca
    16441 tattagtatt catagctggt gctataagaa cctgtgcttt tgttgccata acagtagttg
    16501 tcaacatatc gtcagctata ccatttgcaa tcttgcctat aacatttgca cttgctggca
    16561 ctattaagaa taaatctgct ttttttgcta atgatatatg ttcaacttcc caagtttttg
    16621 gttcttcaaa catatcttct actacataat tttgactcag cgattgaaat gtaagtggtt
    16681 taacaaattc tgttgctgat tttgtcatta taacatggat atttgcattt agttttttta
    16741 acttacttac aacatcacaa gccttatatg cagctatacc gccacttatg ccaattacaa
    16801 cagttttatc ttttagcatg accttactcc tcttctgaaa tttgttggtg ttgctcagca
    16861 tgatgtctag cttcttctat ctctatttcc tcttctgtca ataatctata tgttattttt
    16921 ccacttgcaa cttctttagt tgctacacat acaggctttt ccttagtttt atttgaaaca
    16981 tatggttctt ctccatcaat taattttctt gctctctttg aaacagtccc tactaaatag
    17041 tatctgttgt ctattttttc tagtacctca ttgatagatg gttttaacat aattataatt
    17101 cctccttaaa tttctctatt ataatatttt tatatctgct aactttattc ttttcagatg
    17161 atattatacc ttctatttct ttagcagatg tttttacatc ttcattaaat ataaaataat
    17221 cataatcctt aatcatttta atttcttcat atgcacagct aaatctcttc tctatttcag
    17281 cttctgtttc tgtacctctt ccaacaattc tattttttaa ttctttaagt gatggtggta
    17341 gtacaaatat aaatactccc tctgggcaaa cttcttttat ttgttttgct ccttgcattt
    17401 caatctccag taatacatct tgtccttttt ctaaacattc cattattgcc gcttttggag
    17461 ttccataaaa attatcataa atttgagcat actccaaaaa ctcacctttt tctatcatct
    17521 cttcaaactt ttccttactt atgaagaaat aatttactcc atcaacttct ccagttctgg
    17581 gttttcttgt agtagctgat acagaaagtt ttattgtatc attttcttta agtagttctt
    17641 tgcaaatagt accttttcct gcacctgatg gtcctgatac aactagcaat aatccttttt
    17701 tcacaaacat ttaagcattc cccttctaat attgtattct tactctatat tttgtatttg
    17761 ttctcttatt ttttccaatt cgctttttac ttctactact aaattagtta tatttatatc
    17821 agatgacttc gaacctatag tgttagtttc tctattcatc tcttgtatta agaaatctat
    17881 ttttctacct atagagtcgt ctttaaatat agtattttta agttgcccta tatgactttt
    17941 aaatctaact atttcttctg ttatactgct tttatctgca tatattgcaa cttcttgtgc
    18001 taatctattc tcatctatta tacttgggtc atccagtaat tcacttattc tcaaattcaa
    18061 tttttctcta taatcttcta caacactact tgaatatttt tcaatctctt ctatatgatt
    18121 ttttaataaa tcacatctca tggcaatatc ttcagctaat tttttacctt cttcacttct
    18181 catttctctt aatttgatta atgcatcttc aactgcttga ttcaacattg accacaataa
    18241 atcttcatct tcttcctttt cttctatttt cacaatatca ggaaactttg caacattcat
    18301 aacgctaata tcatctataa tatcaaattt attttttatt tcttttagta tgtctatata
    18361 ctgagatgct aatccctcat caaaatttaa gtttacatct tctttgccta ataagtcaaa
    18421 ctttatgtat aaatctactc ttcctctttt tacataattt tttactaaat ttctaacttt
    18481 atcctctaaa aatgaaattt ttcttggaag acgtatgttt atatctgaat acttatgatt
    18541 tatcgtttta cactctacta aaaagtaata attatcatcc ttgtattctc ctcttccaaa
    18601 accagtcata cttatagcca ttaattaaac ctccaaaata ccttcacata tttttactgc
    18661 tggacctgtc atataagaga aatctcctac atctatttta actgttccac cttgagacgt
    18721 tacatttaca ctactttcta ccatattaag taaattgcac actattgctg atgcagtcat
    18781 accagtacca caacctaaag tatatccaca acctctttcc caagtataaa catgtatatt
    18841 atacttatct tctaccttta caaaatttac attcgttttt tcaggaaata ttttattatt
    18901 ttcaatctct tttccatatt tacaaacatc atctacatct aattcatcta ctataactac
    18961 tgtatgagga acccccatca acatagaagt aactttaagc tccctatcta aaacctttat
    19021 actttcttgc acaaaacgtt ctttatttgt aagtacagga acgtccttag ctataaaact
    19081 tccttttccc atattgactc ttatagagtt tatttctcta ttagtaagat ttacttttat
    19141 atcttttata ccatctaatg tatctactgt aaaactttct tttttaacta tacaattatc
    19201 ataaacaaat ttacaaaaac atctaaggcc atttccacac atagctgctc ttgaaccatc
    19261 agaattataa tataccattt ctacatctgc aacttttgaa tctttcacaa ctaacaatcc
    19321 atccgctcct acagaaaatc ttctatggca tacttttttt gctaaagcag tataatcaga
    19381 tggatttatc tggtcgaatc ttccatctat tgctataaaa tcatttccta ttccatgtag
    19441 tttccaaaat ttcataagta tcgctccttc gtaaattaac acttaaacta ttataacata
    19501 aaaaaattcc cataaactta taatttatgg gaaaagtttt gtaatgacaa ctataatttg
    19561 aggatgtgag ggagagttgc cattacaatt agggtaatta tattatgttt ttaagctagt
    19621 tctttctatg tttatatatt aatatttcta tatgaatttt atgtatgttt tatttaaact
    19681 gtctatgaat tttttaatta aacatctggt aactagctat aatccttcta gtagatatct
    19741 attaacatac acctattaat tctttaagtg tttttaaaac atagtctaaa tcttccttag
    19801 taatatctac atgagttaca aatctatatt caccattttc cattttatta atttttatat
    19861 ttctttcaaa aaattctttt attaaaacat cctcttttat aatatcttca ctcatcttaa
    19921 aaaataccat acttatatct ggattaacat tgtttacttc aatgccatca attttattta
    19981 attcacaagc taaatatttt gcattttcat ggtcttcatt caatcttaaa gtcatttttt
    20041 caatggcaat tataccagct gctgcaacta tacctacttg tctcattcct ccacccataa
    20101 ctttttgctt tcttctagct tttgctataa attctttatt tccagcaagt atagaaccca
    20161 ttggtgcagc taaacctttt gacacacaga acataacact atcacaacac tttgctattt
    20221 ctttagcttc tacacccaaa gttaatgctg cgttaaataa tcttgctcca tctaaatgta
    20281 ctggtaagtt atgtttctca gctatttttt taatttctct taaattatca agactcacaa
    20341 ctgttccacc tgaatatgca ttttctacac atataagccc tgtctgtggt tcatgtatgt
    20401 catctcttct aattgccttt tcaacaagtt caggatttag agctccactt tcgtcttcat
    20461 ctatagttct aagctgaact ccagctatta cagcaggtgc cccaacctca tgagtcacaa
    20521 tgtgataacc tttaccaata attatttctt gccctcttag gcaatgagta aatagagcta
    20581 attgattccc aaaagttcca cttggaagga aaatagcatc ttcttttcca actttagctg
    20641 ctgctaattc ttcaagttta tttacagtag ggtcatctct aaagattgaa tctccaacag
    20701 gagcctttgc catagcttct ctcatttcgt cagtaggcat agtaacagta tcacttctta
    20761 aatcgataaa tctcattttt tcaaccttct ttcatattta tatactatag aaccttaaaa
    20821 ttgatttttt ctgtaaagtt catatacttt catagcttat actctacaca ataattatac
    20881 tatatttact ttatatttaa tctactttaa attattaatt ttaaaaataa agtttcctct
    20941 ctaataaata taaataatta tacaatttaa atattaatct ctattgttta aattccccct
    21001 agtcacaaag cgtatattat gtgtataatt aatttactga ctcattttgg tcaaagaaag
    21061 gggtgctata aatggcatta gatggattag ttatacattc aatagtcgat gaattatcat
    21121 caaaacttac aggtggaaaa atagataaga tacatcaacc tgaagatgat gaggtaattt
    21181 ttaatataag aaataataaa gaaaatttta gattggtttt aagtgcaagt gcctcaaatc
    21241 ctagagttta tcttacaagt aattatcaaa aagaaaaccc tctaaaagcg cctatgtttt
    21301 gtatgttact tagaaaatac atacaaggtg gaaatatagt agaaatttct caaataggtt
    21361 ttgaaagaat aataaaaata agtgttgaat ctttagatga attaaaagaa aagacagtta
    21421 aaaatattat gattgaaatt atgggaagac atagcaacat aataataact catggtgaag
    21481 aaaataaaat tatagattct ataaaaagag tcccttttag cataagtaga gttcgtcaag
    21541 ttttacctgg tcatgattat tcattgccac cagaacaaaa caaactcaac cctcttgatg
    21601 atatatctaa ggatttgttt attaaaaacc tagaggaatt ggaaggacct atctttaagt
    21661 ctatatattc taaatttcta ggtataagcc ctattatagc taaagaaatt tgctacagag
    21721 caggtgtaaa tcaaaatgct atcataaagg atatatctga tgaacaattt gattctttac
    21781 acaaagtatt ttgtaattta ttcaatgata taaattctaa caaatactct ccttgcatta
    21841 ttatagataa aaaggtggat aaggtagttg atttcagttg tataaacctt acattattta
    21901 gcgatttatc atatataaat aaggatagta tgtctcgtat tctggaagat ttctatagaa
    21961 ctaaggatat taaagataga attaatcaac gttcatctga tttaaagaag agtatatctg
    22021 ttaagcttga tagactttat aataaactta aaaaacaaga agaagaatta tcagaatccg
    22081 aaaatgcaga catttataag ataaaaggag aattgattac ttcatatatt tatatggtag
    22141 aaaaaggtat ggaaagtatt gaagttgcta acttctatga tgaaaattgt aatgatgtaa
    22201 caatagaatt aaataaaaac ttgacaccat ctgaaaatgc tcaaaaatac tttaaaaagt
    22261 ataacaaaat gaaacatgct aaagtcgaga tatctcatca aatttcctta aataaggaag
    22321 aaatagatta cttagaaaac ataattttaa gtattgaaaa ctgtgaaaat ttagctgaat
    22381 tacaagatat aaaagaagaa cttgctaagg ttggatacat caagactcaa aagaaaaaca
    22441 gtaaaaagga tactatccca tctacaaaac ctcatgaatt tgtttcttct gatggattta
    22501 aaatattggt tggtaaaaac aataagcaaa atgactatct tacactaaga cttgctgata
    22561 atgatgactt atggatgcat acaaagaata ttcctggttc tcatgtaata ataaagtgtg
    22621 ctggtaagga agtccctgat aatactgtat ttgaaggagc tatgctagca gcattcttta
    22681 gtaagtcaaa actttcttct caggtgcctg tagactatac taagagaaaa aatgtgaaaa
    22741 aacctagtgg ttctaaacca ggtatggtta tttatgaaac taacagtact atttatgtta
    22801 ctcctgaaga agaaacagta gccaagctta aggttaaatc tgaataaatt ctgcactaaa
    22861 tacaaaataa atgttagaca actacacatt atcattctaa ataaaaatag tggccttctt
    22921 ataaagtaca ataataaggc cactttctaa ttagcttact tttaattttc taacaaaata
    22981 cttttctgtt ttaataacta tacttatatt cccaacaaat attttcagtc ttcttatata
    23041 gatgtaatta aaaagttaac actaaaagct attttttaat ttattgattt gttttagtaa
    23101 ttcattatat tctgattcat acaattcttt ttttagatta tctttttcta tagataacat
    23161 agatataaca gttgtaagtc tattttctaa tactagaaga ttttcttttt tgatttggtt
    23221 ttctctacta ggagtttttt tatttctgct aattgtatat tcgtcataat tacaatcaaa
    23281 ctcccttatt ttagcatctt ttatctctat aatatagtta caaatatgtg atacaaatac
    23341 tctatcatga gacactataa gcatagtttt atcagtattt atcagtgcct tttccagtgc
    23401 ttccatagat tttatatcta agtaatttgt tggttcatca aatactaaaa aattattatc
    23461 ttccaatatt attttacata gtgctatttt taccttttct ccaccactaa gtacttttac
    23521 ttttttgtag acatcatcac ctttaaaccc aaataggctt aagtttatcc ttataaaaga
    23581 ttcatcaaat gaactattaa ctttagtatt gtatagaaca ctcttttcct catctaataa
    23641 actttgattt tggtcaaaat aacctacttt taccttgtta tttattttta tattgtcatt
    23701 tttatcagcc aatatttctt ttatcagagt actttttcca cacccattat cacctaataa
    23761 tgctatcttt ttacctcttt ttatcttaaa ggaaacatta tctaacagga gcttattttc
    23821 taactttaga gtcatatctt ttacctctac tagattttta ctaattatct ccataccatc
    23881 ttgaatctta atgttcattt ctttagaaac tttaggttta ttctttacct caagtttatc
    23941 aattctgctt tttatagcct tcacatttga gtccaacttt ttcttattag tctgaccacc
    24001 cattttgtgc aacctagctt ctgaattacc cattcttttt ggtgtttttc ttatgccatc
    24061 acttaaagct tttttatata aaagagcttt ttcaagcctt tttttctctt taacatattc
    24121 ttgatactca aaatcagctc tttttacttc ttcatctttt tgcattaaat aatcagtgta
    24181 attgcctttg taagctttta gttttccatc ttctagttct aatatagttg tacataactc
    24241 atccagaaaa cgtctatcat gagatattaa taataatgct ccctcatgcc ttttaaacat
    24301 atcttccaaa accccaatgc tttttttatc taagtttgaa gttggctcat cagctattat
    24361 gatatttttc ttattactca gcgcatctgc tatttttaac ttaacctttt cccctcctga
    24421 taagtgttct tcaaatttat ctggagcatt taaaagactc ttctccttac tatgactaca
    24481 tgtttcaata ttattttcac tctgacttat ataagaatag ctttctgtaa ggtaagtata
    24541 tccttcatca atttctattt ctcccaatat tgcttttaat aatgttgttt ttccttgacc
    24601 atttgaacca actagaccta ttttgtcatt ttctaatatt tcaagtttat ctatatccaa
    24661 aattaattta tctgcataat atttctttaa attctctact tttactaaca acataaaaaa
    24721 tccctcctta acacacttaa atataaggag agattctata aataggcata acaaataaac
    24781 gtatagacta taatctactc tccttaattt aagcattgca aataaagcca ccaaaatagg
    24841 cttttcaaaa attacaatac tttaaattat aaaaaataga ttatattctc attgtcctag
    24901 ttaccttgcc ctttctttca tttattttta atcattatat caacattaat tacaaatgtc
    24961 tatagaaaat taactaattt tagttattca atatacaact tattctttaa atttattctt
    25021 cttcaacttt aaagtcctga ttatttaaaa ttctgtttaa tacaattcca acaattgcag
    25081 ctaaactaag accagttatc tttactgtac ttgttatagg gattccaaca gaaatacctt
    25141 tattagataa atatgttgtt ccaagaccta ttaataatat aactgctatg attaatatat
    25201 ttattttact ttctacacaa gaactgtccc ttatagtttt taatcctaca taagttatca
    25261 tcgaaaatag cattatacta attccaccca taactgaacc tggtatactt tgaagaaatc
    25321 caccaaactt gcctacacat gcaagcaata tagcaaatat tgctgtacgt ctaagtattg
    25381 aagggtcata gtttttagtt atagcaagta ctgctgtatt ttctccataa gttgtatttg
    25441 ctggtcctcc taaacaccct gcaactatag tcgcaaatcc atccccaagt aatgttctat
    25501 ttaatccagg attttctata aagtttttac ctactacagc accattagta gtcatatctc
    25561 ctatatgttc cataaataca gctaatacaa ctggagatat aatcactatt gctcctaaac
    25621 tgaacttagg aactgtaaag ttaggtattg caaataaact tgcttctgta atagtagtta
    25681 tatctactaa tcttaatatc aaacagatta tatacccaga aaaaactgct attaatattc
    25741 ctaattgttt tataaatcct ctaccaaact tattggttaa aattgctatt gctaaagtta
    25801 taaatgctat cacaaaattg gctgaagcca tactaaaagc tgttggcaat aaatttaatc
    25861 ctataactac tatcatagct cctgtaactt gtgcaggaaa atataattta attttattta
    25921 caccaactac ttttactata atcgacatta taatataaat taatcctgct actattatac
    25981 caccttgagc atatgctaaa tctccaccat acgcctcttt agctgctagt ataactggta
    26041 taaacgcaaa acttgaacct aaaaaggcag gtacttttcc ttctgtacaa aaatgaaata
    26101 tcaatgtacc aacaccagca cagaaaattg caactgacgg attaaaacct gtcaaaatgg
    26161 gaactaatac tgtcgctcca aacatggcta atagatgttg taaagacatt atcgtttttt
    26221 tcataaaaac ctccaaaatt attatatata aataaatcct ctgtttaatt aatacctttt
    26281 aattaattat cattccctag attctatttt ttaacctcta tatacttatt atataattta
    26341 tcattatttt acattttacg acatcaaaaa ctcaaataaa ggagaataaa aactacatta
    26401 agactcacat ccataaaatt ataatattta ttttgtgcaa tctatgtaag ctttatcctc
    26461 ctccctgact attcaatgct aagcttttat ataatactta aaaataatta ttctttgatt
    26521 gtaactgaat cttctccatc aatttctaat aaactgacag atataatttc atgtctagaa
    26581 gtaggtacat tttttcctac ataatctgct cttataggca actctctatg ccctctgtcc
    26641 actagcactg ctaattgtat tgatttaggt cttcctatat ctataatagc atctagtgaa
    26701 gctctcacag ttcttccagt gtataaaaca tcatcaacta aaactactgt cttatcattt
    26761 acattaaaat ctaaataagt tcctttaact acaggttcaa catgaatttt ttccaaatca
    26821 tctctataca aagttatatc aatatctcct gtatctactt ttgttccttc tatttgttct
    26881 attttcttag aaattctatt tgcaattggc acacctctag tctttattcc aactaaaact
    26941 agattttcta cacctttatt tctttcaatt atttcatgac ttattctagt aattgctctt
    27001 gctattgcct tttcatccat taattgagct ttttctacca tatcataatt attgtcaaag
    27061 atataatata tctatttgac aaatctactc ctttctttta attttttata aatttatttt
    27121 tatttaaaat ttcaatttaa acaacaaaaa aacttctcat cacaagacaa gaagctctta
    27181 tataaaaaat atacagtgta atttagcaat tgtcttacga tatctctgta tcgtttaaag
    27241 ataatttttt aatctatcaa ctcaaattat atatttatat ttcaaatttt tcaagctttt
    27301 ttaaacaaaa tactttaata taagatattt tatttaaatt tcactaaatt tatttactta
    27361 tataaaaaca aaaaatgatt tataagtatt atacaaatgg ttattttatc tttttaataa
    27421 cttccttaaa atactcaggt aattctgaat taaattcgat atattcctta gtagtaggat
    27481 gtataaatcc caacttctta gcgtgcaaag tttgtccttt taatttaaat ttagtttctt
    27541 ttggaccata tacataatca cctaaaagtg ggtgatttat agacaaagca tgaactctta
    27601 tttgatgagt tctaccagtt tcaagcctag ctctcatatg tgtaaactta tcaaatcttt
    27661 ctacaacttc aaagtgagtg attgcatttt ttccatcttt tactacagcc atttttaatc
    27721 tatccttagg atttctccct ataggtttat caacagttat tttatcttct ttaaccacac
    27781 catagcatat aaattcatac tccctagtta tagtatgttc ctttaactgt tctgctagaa
    27841 aattatgtgc ataattgttc ttagctatca taagaagacc agaagtatct ttgtctattc
    27901 tatgaactat accaggtcta ataactccat ttattgatga caacttatct ttacaatgat
    27961 ataaaatccc atttactaac gtattttcat agtttccagg agcaggatgg acaaccatat
    28021 cctgaggttt atttattatt aagacatcat catcttcata tacaatatct attggaatat
    28081 cttgaggtaa aacttctaat actttaggtt ctggtatctc tataacaata ttatcttcct
    28141 ctttaaccag atatctagct ttttctactt tattattaac ctttactttt ccatctttta
    28201 taattttttg tatataactt cttgacatat cacctaattg ttctgctaga taaatatcaa
    28261 gtctaacttc ttcttcctct tcaagtacta aaaactcttt tacttcatcc attatatcac
    28321 ctacttttac tttcaaaaaa taaaacatat atacataaaa acacagttcc tacaactaca
    28381 aatacatcag ctacattgaa tacatattcc catataactc taaagtcgaa gtaatctacc
    28441 acaaaaccta atcgtactct atcaactaga tttcccaatg caccagatat aattaatata
    28501 attcccaacc ttccaaatat atgtactttc cttgtattaa gatagtataa tccaaatacc
    28561 gttgcaagta atgcaacaat tataaatatc cattgattat tttgtaataa tccaaatgct
    28621 gcacctctat tttcgacata agttaaatga aatacattat ttattattgg tattgttgat
    28681 acatccacca aattattcaa tacccatatt ttagacagtt ggtctaaacc tatgagtaga
    28741 attattatta atatatatag caatgatttt tccccctata aattatttaa aaaccaattt
    28801 tatttgaagc acactattat tattatacaa aaaaaatgct ctaaagagca actttattaa
    28861 aattgtattt tcttaataaa gctagaacaa aatttaaaat agtactactt tttttatatt
    28921 gtagatttct acatatttaa aatcatatca ttatgtataa aaaatttaaa aataccatac
    28981 taatcaatat tttttgtgta ttttatagat taaagattaa tgatgtctgt aatttaaact
    29041 tacaatcacc actaatcttc aaacaaatat ttttactttt gcacttgctt atttttatct
    29101 aaatgttgta taagttcaga tactgttacg aatttatatc ctctctcttt taatttagga
    29161 attactactt ctaaagcttg tatagtttgt gatttatcat ttcttttagt gttataatca
    29221 tgtaaaagta ttatattacc attttggacc tttgtaagga tagtatttat aattgcatct
    29281 actccagggt tagaccaatc tctagggtct aagtttgacc aaagaattat attcattcct
    29341 ttagaaacta taatatcaca gacactttta ctcatggctc tatatggagg tctaaatatc
    29401 tttggttcta ctcctatcac actttttata atattttgcg tatctgttat ttctttttca
    29461 acactactat aactattttt tgcaacattt atatgtgtaa atgtatgatt ccctatttca
    29521 tgcccctcat catattgtct ttttaataat tctttgttat attctacttt ttctccaact
    29581 acaaaaaaag ttgcttttac atcatttttc tttaatatat caagtacttg tggtgtaaaa
    29641 tcctcatctg gaccatcatc aaaagtcaat gctaaaattt tttcatcctc atttcctttt
    29701 tttattatta catcttcata atcgtctttt atattgtcat ttaaactata tgcttctttt
    29761 gaactatctt gattatgtgt attatgatta tatgtatata cacctactat agataataaa
    29821 catatacata taccacatat tattttcttc atgtcattac ctcttttcta attcattcta
    29881 gcaatatatt ttataatgtc agttttttaa tctctcaata ttccattttt atttaaatca
    29941 gttaaatttg ttaggtcttc tgaataaaag ttagaatctt ctacatcagg gttttgcatt
    30001 gcatcaccta ataaattatt ttccttcttt gcacaatcat tacataaact tgtatctggc
    30061 aatatatcca atctttctaa atctatttga ttatgacata aatcacaaat cccatatgtt
    30121 ccattctcta ttttagataa tgctaagtct atttgataca atcttccctc ttcatgattt
    30181 attagactgt tttgcatatt ttgcatatat aaatctgttc ccatgtctcc tatatgatta
    30241 tcataactag ataattctcc tgatgaatac ttttcacttg tatgtttagt tgtatctcca
    30301 aataaagtat tatctttcat atcctctact aaattagtta aattttctct ttctttcaat
    30361 aatttatctt tatatttttt agtatccatt tttaacacct ctaattttaa tatttacatc
    30421 ataaaaatag tatgtggaaa agtaaattaa acatacaata aaaataagat ttgattcgaa
    30481 aaatggaatg tctcaaaatg aaatttttag ttcatttaac cctctttttt atatgaaatc
    30541 aaatatattt ttatcctttc agcaataaaa aagagactta tctctatttt gagatagtct
    30601 cttctttgaa cataatttaa atacaatttt ttaattttta tagttattat tttactaact
    30661 tgccaattta tgattatttt gttctttaat atgttttgct tttttaaaga ataaaagacc
    30721 aagaattatt gctacaataa cacctattcc attagatata ttaactggta gtctaaatcc
    30781 ttcttttgct tgcattatat aactaaatgt tactacagtc ataaaaagtg ctggtaatgt
    30841 agttatataa tggtttttat ttgctttgac taaatatgca gaaccagtcc acaacattat
    30901 cattgcaagt gtttgatttg cccaagagaa atatctccaa attatattaa aatctataaa
    30961 tgtaagagca acaccaactg cgaataatgg taaagctatt aaaaatctat ttttgactgc
    31021 atcttgatta taattcattg catctgctat tgtcagccta gcacttctaa atgctgtatc
    31081 tccagaagtt ataggacatg ccacaactcc aagtatagct aaagctcctc caataggccc
    31141 cattaatcca acagatactg tatttactac tgttgctgct ccaccatcat taaatatcac
    31201 attaagttga ggtatgccat ggaaatatga catggctgct gctgcccaaa ctaaagctat
    31261 tataccttct gcaaccattg acccataaaa tactggtctt gcctcactct ctttctcaac
    31321 acatctcgcc ataagtggtg attgagttgc atgaaaacct gatattgctc cacatgctat
    31381 tgttataaat aagtatggga ataaaggtgt tccatttgga ttcatatttt gtaaatttat
    31441 atttggtatg ttatacccct ttattattaa tcctccagct acacctactg ccataattaa
    31501 taatgctgct ccaaataaag gatatatttt accaatcaat ttatctattg gtaaaacagt
    31561 agccataata taataaagta ttatcactcc aacccatatt tgtgcgttta cacctgtcaa
    31621 tgtctttaat aatcctgctg gacctgttac aaatacaact ccaaccaata ctaaaagaat
    31681 aacactaaat attctcattg ctatttttgc accatttcct agataaaggc ctaccaactc
    31741 tgatgcagat gtacctttgt ttttcattga tagatatcct gaagcaaaat cgtgtactcc
    31801 accagcaaag attgttccaa acacaatcca taaaaatgct gatggtccaa ataatgctcc
    31861 ttgtatagct ccaaatatcg gacctaaccc tgctatattt agaaactgaa ttaaaaagat
    31921 tctcttccat ttcattggca cataatctac tccatcccta caagctatag ctggagtttg
    31981 atttttgtca ttggttccaa aaaccttttc tacaaaagta ccatagacaa aataacctac
    32041 tataagtact actattgaac ctaaaaagct taccataaat tcatcccctt agtttttata
    32101 taattgcttt ataaattttt atgagtttaa gaaaactatt tccagttcaa atagtaatac
    32161 ttttttatgc taatataaat attaattaca ttaagtgcat atttaagtac atgaaatgac
    32221 tttatatatg cattaacagc ctaaattctt ttactttgct tctacttaca gttacttcat
    32281 aatcactatt ttttaatttt aatatgtatg tattattaaa ccatggtata atttctttta
    32341 ctttggttaa gtttacgata taagacctat gtgttctaaa aaatgtttta tcatttataa
    32401 gattttcgac ttcttatatg ccttctttta atataaactt ttctttctca gtatatataa
    32461 atgtctgcct ttcattagct tcacagtaat atatatcatc aatatctatt acaactaatt
    32521 tatcaccttt ccaaaggcta attttatgtg ttatttcttc tatctcctga tttactgctt
    32581 ccttcttata tttgtataaa ttactattta cattaaatag ttcaatatca ctcatctcac
    32641 ttttttctaa tttattcagc atagaaataa ttctctcttc agaatatggt ttcaatatat
    32701 aatcaaatac atccaaagaa aaagcatcta ctgcataact ttcatatgct gttataaaaa
    32761 caattttagg tcttgattta aactgattta atgtttttgc aagtagcatt ccatcaagat
    32821 gaggtatatt tatatctaaa aaaattacat ctattttatt ttcctgaata aagtctaaca
    32881 cctctatacc attggtaaat tcacttacta cctcgattcc acttttattt tcaataaaat
    32941 atctcaattc ctttcttgct ggaaattcat cttccactat tatagctctc atacttctac
    33001 tcctttacat aaaatactat caaagtccca ttatctattc ttcttatatt aagccctctt
    33061 ccatacatca gtttaagcct taaatgtaca ttataaagcc caatcttatt ttctggcata
    33121 ttatcttgat atacattatt tataatttcc tcacttatac ctataccatt atcctctatt
    33181 gatattctca cagtattttc taatgaatac ggtaactttt ttatggatat tttcactaca
    33241 ccagctctac cactttctaa aataccatgt attattgcat tttccactaa tggttgtata
    33301 atcaaacttg gaatctttat atttacatct tctatatcat aagatacagt tagtaattct
    33361 ccaaacctag ctttttctat ctctacaaaa gatttaactt gttcaatctc tttatttata
    33421 tcaattaagt tatcactata ttcaagatta tatcttagat aagttgataa atttattatt
    33481 aactctctag ccttatctgg actaaatctt ataaatgaag ctattgtgtt tagggaatta
    33541 aacaaaaaat gtggattaat ttgtctttga agtgctttta tttctgcttt atttctcgct
    33601 tctatatctt ttttatcttt ctcaatcccc tctactatag atataacaaa tcctattcca
    33661 atctgcccaa ctattaaagg taaatatatt ccaccgatta tttgaattgc taaagaatgt
    33721 ggatatgata ttaagtatat aagtaatata gtaaaacttt cagaaatcat gcctaccaat
    33781 attccataca ttactctatg ttgttttggt atcctcttat atagaaatcc tgataataca
    33841 cctgctagta tacttgataa aagacatgga acagatgtta tccccccaat atccataaaa
    33901 tatctatgaa tccctgaaaa aactcctgct gtaataccta ccatcggacc aaataaaatg
    33961 ccacctgata ctatagatat tattctagtg tttactattg accccatata atttatacca
    34021 ctataagttc caaatatggc taaaaaagta aaaactaatg ctatacagac caagtcatta
    34081 aaactatatt cttccttttg aaatatctgc ttaaatattt ttaactttgt tattagaaga
    34141 aacaatacta caataaggct tgtcttttct attaatgcga ttaaaattga tgttttcata
    34201 atcccccttt aaaaaataaa tttttacaca ctctaaattt attatggttg ttataaatta
    34261 catatttgaa gtaatcctat cacaaaactt tccacattta atcttttaat taaataagaa
    34321 atagttgtac ttctattaac aactaatact agctagcata agcttgtaat aacttgtagt
    34381 tattattaac tttacaattc tagtatttaa ttttatactt tagaaatctc ttttcttaga
    34441 aataatactt tcgaatatca tataatacaa tattatgtag attcttactc atacaacata
    34501 tttctttaaa aattttatac tattctttgt tcaaatatct catttgattt aattgtgtta
    34561 aacacacatt atcatctctc tgttatatat ttattatata gccgtatatc gataaagtaa
    34621 ataaatccca tatttaaaat aatatgtttg acaataaatt attataatgt atatttacca
    34681 ta
//
LOCUS       DAMJZE010000023        33000 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJZE010000023 DAMJZE010000000
VERSION     DAMJZE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.023"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            29..1885
                     /locus_tag="PC541_001030"
     CDS             29..1885
                     /locus_tag="PC541_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2838501.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1968..2208
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2274..2509
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2594..3811
                     /locus_tag="PC541_001031"
     CDS             2594..3811
                     /locus_tag="PC541_001031"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2838502.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3928..4410
                     /gene="cdeM"
                     /locus_tag="PC541_001032"
     CDS             3928..4410
                     /gene="cdeM"
                     /locus_tag="PC541_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2838503.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4674..5939
                     /gene="hisD"
                     /locus_tag="PC541_001033"
     CDS             4674..5939
                     /gene="hisD"
                     /locus_tag="PC541_001033"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2838504.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6142..6894)
                     /locus_tag="PC541_001034"
     CDS             complement(6142..6894)
                     /locus_tag="PC541_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2838505.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7012..7281)
                     /locus_tag="PC541_001035"
     CDS             complement(7012..7281)
                     /locus_tag="PC541_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838506.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7756..8850
                     /locus_tag="PC541_001036"
     CDS             7756..8850
                     /locus_tag="PC541_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2838507.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8852..9802
                     /locus_tag="PC541_001037"
     CDS             8852..9802
                     /locus_tag="PC541_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2838508.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9795..10979
                     /locus_tag="PC541_001038"
     CDS             9795..10979
                     /locus_tag="PC541_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838509.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10992..12488
                     /locus_tag="PC541_001039"
     CDS             10992..12488
                     /locus_tag="PC541_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2838510.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12511..13461
                     /locus_tag="PC541_001040"
     CDS             12511..13461
                     /locus_tag="PC541_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838511.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13482..14444
                     /locus_tag="PC541_001041"
     CDS             13482..14444
                     /locus_tag="PC541_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2838512.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14816..15448
                     /locus_tag="PC541_001042"
     CDS             14816..15448
                     /locus_tag="PC541_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2838513.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15859..15951
                     /gene="kdpF"
                     /locus_tag="PC541_001043"
     CDS             15859..15951
                     /gene="kdpF"
                     /locus_tag="PC541_001043"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2838514.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15992..17674
                     /gene="kdpA"
                     /locus_tag="PC541_001044"
     CDS             15992..17674
                     /gene="kdpA"
                     /locus_tag="PC541_001044"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2838515.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17699..19756
                     /gene="kdpB"
                     /locus_tag="PC541_001045"
     CDS             17699..19756
                     /gene="kdpB"
                     /locus_tag="PC541_001045"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2838516.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19775..20401
                     /locus_tag="PC541_001046"
     CDS             19775..20401
                     /locus_tag="PC541_001046"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2838517.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20638..21546
                     /gene="cysK"
                     /locus_tag="PC541_001047"
     CDS             20638..21546
                     /gene="cysK"
                     /locus_tag="PC541_001047"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2838518.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21573..22163
                     /gene="cysE"
                     /locus_tag="PC541_001048"
     CDS             21573..22163
                     /gene="cysE"
                     /locus_tag="PC541_001048"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2838519.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22323..22511
                     /locus_tag="PC541_001049"
     CDS             22323..22511
                     /locus_tag="PC541_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2838520.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22565..23434)
                     /locus_tag="PC541_001050"
     CDS             complement(22565..23434)
                     /locus_tag="PC541_001050"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2838521.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23631..24875)
                     /locus_tag="PC541_001051"
     CDS             complement(23631..24875)
                     /locus_tag="PC541_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838522.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            25166..25477
                     /locus_tag="PC541_001052"
     CDS             25166..25477
                     /locus_tag="PC541_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2838523.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      25570..25662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            25762..26556
                     /gene="thiD"
                     /locus_tag="PC541_001053"
     CDS             25762..26556
                     /gene="thiD"
                     /locus_tag="PC541_001053"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2838524.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            26580..27368
                     /gene="thiM"
                     /locus_tag="PC541_001054"
     CDS             26580..27368
                     /gene="thiM"
                     /locus_tag="PC541_001054"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2838525.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            27391..28023
                     /gene="thiE"
                     /locus_tag="PC541_001055"
     CDS             27391..28023
                     /gene="thiE"
                     /locus_tag="PC541_001055"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2838526.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            28162..28464
                     /locus_tag="PC541_001056"
     CDS             28162..28464
                     /locus_tag="PC541_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838527.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(28530..29285)
                     /locus_tag="PC541_001057"
     CDS             complement(28530..29285)
                     /locus_tag="PC541_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838528.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(29273..30235)
                     /locus_tag="PC541_001058"
     CDS             complement(29273..30235)
                     /locus_tag="PC541_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838529.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(30239..30697)
                     /locus_tag="PC541_001059"
     CDS             complement(30239..30697)
                     /locus_tag="PC541_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2838530.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            31053..31409
                     /locus_tag="PC541_001060"
     CDS             31053..31409
                     /locus_tag="PC541_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2838531.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            31481..32197
                     /locus_tag="PC541_001061"
     CDS             31481..32197
                     /locus_tag="PC541_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838532.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            32190..>33000
                     /locus_tag="PC541_001062"
     CDS             32190..>33000
                     /locus_tag="PC541_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838533.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKK"
BASE COUNT        12731 a         3594 c         5795 g        10880 t
ORIGIN      
        1 aataacattg aaaggtagtg gttagaatat ggatacccat aataaatatg taaattttat
       61 aaaaaatata cctgtacctt ttttatattg tagaattgta aagagacaag aagatataga
      121 atatcgagta gaatatataa gtaagggtat gggcaaagta ttacaattag aagaaggtat
      181 ttgtgataaa aatatactag atgtattgcc tgtatttaag tctaaaaaat atttcaaaga
      241 gttgttctca aatgaagtgg attgtataaa aaggtacatt ccgacactta aaaattggat
      301 aaatataaaa aaacagataa taggggattc atacataatt ttgtattttg gtaaaattgt
      361 atttgattat agacagataa tcgattcctt tgataaaaag gaaaaagtag cttatattaa
      421 agacgaagaa ggcacatata tagattgcag tgaaaattta ataccaatat taaataacaa
      481 cattaaaaca acgaaagata tctttggtaa aaatgatata gaagtatggg gagagaatac
      541 aggaaaatta tttagagatg attatagaga aggagtatct agtaaaaaaa gatttttaca
      601 aaatcttttt gaatatgaag aaacattttt tatggttgaa aaatattttt tgtatgatga
      661 agacgaactt ttaggtacta tagggatagt agacaatata atatatagtg gttacagtaa
      721 tagaaattat aactcaaaag atttaatgaa gatgatagaa cactcaattc cagagaatat
      781 gttttataaa gatgtgtatg gaaattatat tggatttaat tcaggttttt tgaatttagc
      841 ttgtatgaat aaagaagaat tattagggaa gaatagttat aaaatatcag aagaagaagc
      901 tttaatagat aagatatttg aaagtgataa gggtgtagtt gaaaataaaa aagtagttac
      961 atttgagtta aatataagta tgaatgatga aaacaagtgt attgagatta caaaaaggcc
     1021 gttttttgat agctatggaa gtgttattgg aataattgga acagcaaggg atataagtag
     1081 aagaaaaaga ttagaagagg aaatggataa aactaggatg gaattttttg caaatttatc
     1141 ccatgaactt agaacgccta taaacttaat atcatcttca ttacaagtga tagaaaaaaa
     1201 agaagcagat ttaatagaat caaatgatac tttaaaaaga aatttaggta taataaaaca
     1261 aaatggaaat agaatattaa gattagtaaa taatgttata gattttacaa aaatgcaatc
     1321 aggatattta gattttaaac cagaagaatc tgatataatt gcatttatag aggagatatg
     1381 tatgtctgta gctgattttg caagccaaaa taatatacaa cttacatttg atacagaaat
     1441 agaagaattt tcaatgttat ttgattcaga aaaattggaa agaataatat taaatttact
     1501 ttctaatgga attaagtaca ataagaaaga tggaaaaata aatatatttt tatatgtgaa
     1561 agataatgta tttaatatga aaatatctga tagtggaata ggaataccaa aagaaaaaat
     1621 agataaaata tttaataggt ttgaacagat tgacaatgaa ctttcttaca gagttaaagg
     1681 aagtggtatt ggactttcat tagtgaaatc tttggttgag ttgcatgagg gaagtatatc
     1741 tttaaaaagt caacttggta ttggaagtga atttatagtt tcattgcctg ttagaagtaa
     1801 aaataatatt gaaaaatata atcataaaag agaaatcagc aatgaattaa gtaaaaaatt
     1861 agaaatagag ttctcggatt tataagttta aatataaaaa ataaaatttt aaaaaagtat
     1921 tgacaattga tttagaaggg tttaaaataa attacaacat aataaataaa aaggcgatga
     1981 ataggaagag taaatctaat tcgattacca gagagaagag tccgttggct ggaagacttt
     2041 ttaaattaga gggattgaaa actacctagg agctactttc tgaagtcatc tgcattagta
     2101 gattttagta agaatagtcg ttagctgcgt taaagtgtaa agtgagtttt catgacatat
     2161 gaaaactaaa ttaggtggaa ccgcgggaat aatactctcg tccttatgtt ataaggatga
     2221 gagttttttg ttatttaaaa atataaacag taatcataat aattatagaa aatataaaag
     2281 caatgaatag gaagagtaaa tttaattcga ctatcagaga ggaaagttca caggctgaaa
     2341 gactttctaa gtttgagaaa ttgaaaacta cctaggagct gtggtctgaa gttactttaa
     2401 aaagttttag taagactaac cgttaactgc gttaaagtgt aaagtggatt ttatcaataa
     2461 atgataaaat caaattgggt ggaaccgcga gtatagactc gtccctttat atagggatga
     2521 gtctattttt attgcaataa aagattgaaa ataaaaaaaa atcggaaaat tataaattta
     2581 ggaggaaaat aaaatggaga ataaagtgaa aattggagta ctaggatatg gagttgtagg
     2641 aagtggactt atagatataa tagataataa taaagaaaaa agaaatatag aaatagttgg
     2701 aattttagtt aataatttgg aaaaacataa ggataaaaaa tattctaata taataactaa
     2761 taatatagat gatatattta ataaagatat agatatactt gttgaagtca tgggtggttt
     2821 agaaccatca ttatcttata taaaaaaagc attaaacaat aaaattcatg ttgttacagc
     2881 aaataaagat ttgttagctg aatgtggaga tgagcttgca aaattagcaa gtgaaaataa
     2941 agtaagtatt aaatttgaag cttcagttgc aggaggtata ccagtattaa aaccaataat
     3001 agaatcacta gaaggaaata atatagatag tataaatgca attttaaatg gaactactaa
     3061 ttttatatta tctaaaatgt atgatgagaa tctatcttat gatatggcat taagacaagc
     3121 acaagaatta ggttttgctg aagcaaatcc agagtcagat gtattagggt atgatgctgc
     3181 tagaaaacta tcaatattat caactctagc ttatgacaat agagtatatt ggaaagatgt
     3241 gtatttagaa ggaattacag atatagatga aaaagatatt gaatatgcaa aaaaattaaa
     3301 ttgtaaaata aaattgattg gtcaaagtaa gtatgaaaat gataaagtaa gtgcttttgt
     3361 tagacctgtt ttagtagaaa aagataatat ccttgctaga atagacaatg agttcaatgc
     3421 tgtcatagtg aatggagatt cagtaggaga agtttctttt gtaggtaaag gagctggaag
     3481 tttagcaaca ggaagtgcgg tttattcaga tgttatagat ataatagata atagagtttc
     3541 aagtatagat tcttttacta aagataaaat acaagttaat aaaatagtta gagaaaagtg
     3601 tggagcactt cttagattta aaaaatgtaa taaagatgaa attctaaata tagttgagaa
     3661 ttgtttagta aaattcgata ttttaaatga tgatgatgaa cttgctatta tggtttatgc
     3721 tgattctgaa tatgaaataa acaacagttt atgtctaata aaagataaag gatactgcga
     3781 aaaaatgaat aaaatgttga aaataagtta aaaaagaaaa aatataagtt tatatgtaac
     3841 atattttgac gattttaata ctatatagta tgagatgaaa caaaacatct cataaaaaaa
     3901 ttaaaattaa agggagagat atgttatatg gaaaataaaa aatgttattc agaagattgg
     3961 tatgaaagag gagaatctac agctaaatgg ttccaaaatg atagagaaga atatgaaaga
     4021 gaagcatatg atgaagatag agaaagaaga ggttcaaact gtggatgttc agattcagga
     4081 gaaaatagac ctagaaactg tgaaagattt agaagagaag ctgagataag agaaagagaa
     4141 gcaagagaag cattctgtga atcttcagag aaaaagaaag aggcattagc atatgaatgt
     4201 gaagctagaa aattatggga agaagcagaa aaatactggg atgaatattc aaaatacaac
     4261 tataaaggaa tcgaatattt agcagaagct gctagattat ttgatgaagg tatggaatgt
     4321 gaagctagaa gaaatggaaa taatggagga aacaataata attgttgcca taaatgccat
     4381 aaatgtaatt gtaactgctg tagaaaataa aaaaattaat tgacaaatca atagttaaaa
     4441 tatatcatat tatgtataat aaagagaaaa ttcagaaaaa aataatgatt agaagaaata
     4501 taagtatatt tattagacaa atagtgctca gttagtgagg tgagtacaat atgattaaac
     4561 tcccatcctc tagcttttga tagagggtgg gagtttttat ttatttatat atcttaaatg
     4621 aggataatat tgtaaaataa atgagtatga gattgtaaaa ttaggaggta aaaatgaaaa
     4681 gtattaaaac atcaagtatg aaagaaaagg tagatgaaaa gatattaaga gagcaagtaa
     4741 gttctataat agaagatata agaaataata aggatgttgc acttaaaaaa tataatgaga
     4801 agtttgatag aaatacaaga gatgaattta gaattactaa ggaagaaata aaggaagctt
     4861 ataagcatgt agatgacgaa tttataaata acttaaagat tgctgccaaa aatataagag
     4921 aatttgctaa agctcaaaaa ctaagttttg aaaacccttt tgaaaaagaa atttatccag
     4981 gtgtaatctt aggtcaaact aatattccaa tagagtcatg tcttgcatat gttccaggag
     5041 gtcagtatcc attgttttca acagctctta tgcttataat acctgcaaaa gtagcaggag
     5101 taaaaagaat agtggcatgt tcaccaacta tgaaaaatac tgaaaagata aatcctaaga
     5161 cattggtagc tatggatata gcaggtgctg atgaaatata tgcaactgga ggagttcaag
     5221 ctatagcagc atttacatat ggaacagaaa agattaatcc agttgacata attgtaggac
     5281 caggaaataa gtttgtaact gaagctaaaa gacaatgtta tggacaagta gggattgatt
     5341 ttgtcgctgg gccaagtgaa gttcttataa tagcggatga aacttcaaat ccagtttata
     5401 ttgcagctga tttattagca caatgtgagc atgatttaaa tgcaagagga atattattga
     5461 caaatagctt ggaagtagca caaaaagttg aaaaaaatat agaaagtatg ctaaaagatt
     5521 tgccaacaaa ggacatagca tatagttcat gggaaaataa tggagagata atattggttg
     5581 atgatatgga agaagccatt aaaataagta atttttatgc accagaacat ttagaagttg
     5641 cagtaaatga gtataatgat atttgtgata gattaactaa ctatggttct ttatttattg
     5701 gaaatctttc tgctgaggtg tttggtgact atgtttcagg taccaatcat acattaccaa
     5761 cacttaaagc atcaagatat actggtggtg tttgggttgg aacttttatc aaaacttgta
     5821 caaagcagat atttaatgaa aaagctatac aatcattagc acctgtagct gaaaagctgg
     5881 caaaagaaga gggattatat gcacatgcaa aggctgctga ggttaggttt aaaaagtagt
     5941 aaaaaatttt attttggcag tcgagactta gtattagact gagataagga aaacgctgag
     6001 aacctgaata aatactatat tttgtgggtc tcggcgcttc ttttatatct gtatataata
     6061 gaataatact atctgtgtgt ttaaatacta acttaatatt tcttttctaa ataatgttta
     6121 tgaaatagtt aatattttta gttaatactt gaaaaaaact tttgctaact gtgcaaattc
     6181 atctgcattt gtcaatatag cagggtgtcc tccctgacta aaaatgtaaa tacttgcttt
     6241 tgaaataaaa gttgccattt gcttgtactc ttcttcaaga ttatgacgac acatttcgtc
     6301 ttccttactt cctgtaaata aaattggcat ttctaattta cacaattctt tatgaaacaa
     6361 agggcgtttc tcgtttgcac attgcaatag agcttttgta tcaagattaa caacattctc
     6421 ccaatctttt ccatgacacc attcatagaa ttttcgtgct tgaatatctt gctttgattt
     6481 ttcccttcca gtgattagat tatctatgaa gttatcatta agagtacgtc catcaaagct
     6541 atctgctata attgcttcaa ctaaatcggg acgttctaat ccagtattaa cagctgccca
     6601 tgctcctcca cttgttccaa ttaagccaac tttaccacaa ttcaaatgtt caatcagtgc
     6661 tattgtctgt aaggcttcat catgccatat gtcaggagaa aattgagaaa ctctatccga
     6721 ctgtccatta cctaaaaaat caattaagat acatctaaaa ttttctgcat ataatggcac
     6781 aagtgcttta aacatattag aagaaccagt attaccatgc aggaaaatta tcggtttacc
     6841 ttgtccatat tctttataaa aacaagaatt accttgatac tgaaaatatg acatatattt
     6901 tcctcctttt tatttgttaa gttaattata acaatatagg tatgaatgaa aaacctcatg
     6961 ttctttctta gaataaaaat gtgccacgca taaaacgtga cacatttcta attataaatt
     7021 ttgctgttta agtattcttt gtatgctttt ctctgtcaaa aagtatttct ctgataactg
     7081 cttgatactc attccatttt ggaaatttat acagatttga gaattacgag aagctaagac
     7141 ttgtttggtg tttgtgtttt caccccaatg ctttttgtgc tcttgctttt tgggaatata
     7201 aatcacttta ccatctatat attcttgcac ttgctcaatc agttctttag gcagtaagtc
     7261 aatcgaattt tgatagctca ttttgcacct cctaaataat tattctttag gattagcaaa
     7321 ggctactgcc actcacaatt aaaaataaaa ttttttatta tgtacaatag cctttgctat
     7381 gcacaagttc caattcccat aaactaaata ctcctttctg gtttgaaaaa ttcatctgaa
     7441 cattgatttc gcttttaaga tagagttcaa tgacaaattt acatttttat tatatatatt
     7501 cagatagata attgcaactg tctttgtatg aggatatttt attatagtca atactataat
     7561 atgtatttat atcatcaaac taatatgaac agttgaaagg aatatgaaca ataaaactta
     7621 gtcaggaaga ataattatga agcagacaat agagaaattt gattttaaag ttttattgaa
     7681 aatttattga caaatttaac ttgtaaatat tatactaaac ttaataaata aagtttataa
     7741 agtaaggtgt tgtctatgaa aaatatagtt ggaaaaccgc aaacgataaa aaaagtaaat
     7801 gaagatttaa taaaaaacat tataaaaaat gaaggtccta taacaaaacc agaaatttca
     7861 agcattactg aattgagttt agctacagtt aataagattg tagagcaatt agctttgaaa
     7921 aatgaagtta aagtaagtgg actaagtgaa tctactggag gaagaagagc tcaattattt
     7981 gaaataaatg caaacttaga acacataata gctttatatt attatagaaa ttgttgtata
     8041 ggggtagttg ccaatttatt aggtgaaata atttatcaag aagaatttaa tataagaatg
     8101 gatacgtatg aaaacgttaa ccaagatact ttttttgtta tagatactct aataaaaaag
     8161 gaaaaaggaa acaatataag ggctattgga attggtgtcc caggagttgt aaaaaaaggt
     8221 gtagttaaaa atataccaac tataaaaagt tgggaaggta taaatttaga aaatttaata
     8281 aagagtaaat acaatactaa agtgtttata gaaaatgata caaatattac tactattggg
     8341 atttatcaaa aacagtataa agacaaatat aaaaatatgg ctcttatgta tttagaagat
     8401 ggtattggtt caggtatagt tataaatggt gaattgtatt taggttctac aaattttgct
     8461 ggagaattaa gttatctaaa tatagaattt ggtaagtata actctaacaa cttaaatctt
     8521 gaagattata ttatgagttt aagaaaaaga tatttagaaa gtaaagataa taaatttaag
     8581 aaagaaattt tatctataat atcaaacttt ataaggactc ttgtatgtgt cctaaatcca
     8641 caagctatga ttatacagtg tagtatatta accaagagtg acatggagtt tatagaaaaa
     8701 gatgtaagca ataatgttgg agaagaaaat tttcctaaat taataaaagt agactcattg
     8761 agagaaggaa gtataaatgg agtaattagt atgtgtttaa aagaaactga ctatcaatat
     8821 tccttatcta ataaaaaagg aggatattaa tgtgaaatat ttggtttcaa tagatggagg
     8881 gggaacaaaa actaaatttt gtgtaagtga cttagatgga aacatactta aagaacatac
     8941 cactggttca actaattata agtcagttgg aattaaaaaa acatatgaaa atattaataa
     9001 tggatttaaa aaaatattaa aagatttata tatcgattat gacgatattg agtacactgt
     9061 atttggtata tctggttgtg attctcctaa tgattataag attataatgg atgaaatatt
     9121 aaaaattggg attaacaaag aaaaaatata tctggcaaat gatgcagttt tagcttttta
     9181 tgcacaagct gattcccctg gcttagtaat tgtggctggg acaggttcaa ttatactggg
     9241 aattaaagaa gatggagaaa tatatagagt tggtggatgg gggtataatt ttagtgattt
     9301 gggttctgga tatgatatag ggagaaaatt attaaagaaa gtattgcttt attgtgatga
     9361 atgtcatgag tatagtgatt tatttagttg tgtcttggac ttctttggag caaatagttt
     9421 tgaacaactt gcatatatga ttactgatat aaatgataat gttgaaatag caaacttagc
     9481 ttcattagtg attgattgtg ccgaacaagg tgataaattg gctattgaaa tattaagaga
     9541 atcatcaact gaactatcta aattagctca agtaatattg agaaaaatat ctaatccaat
     9601 gaaaatggat aaaagcgaaa taaatatagt tttgtctggt ggtacattaa ataaaactgt
     9661 atatgctcaa atgttaatga ataatttaaa aacagaaaat acagataaaa atttaaaatt
     9721 tatatctcaa gaaaatcagc cagtatatgg ggggataaga cttgctatgg cattagctaa
     9781 taggagggtc aagcatggat agaaaaaagg taatagtaca aatttttact ggaggtttta
     9841 aggctaaaga aataaagttt aatgatataa aatgtaaact actagaatta ttggactgta
     9901 ttgatataga aaaggttatc atgggatgga gtgtagatga agaattatat gtagaaacta
     9961 aaaaactatt aaataaatat aatgttgaac tatatctttg gttgccatgt ttttctgaaa
    10021 taggattact tgaaaaaagt aatttactaa ttgattatat ggggaaggaa gtcaagggct
    10081 atgctttaca agaggatgaa aattttgagt tttattgccc aaataataag gaaaatataa
    10141 aaaatataaa gaaaatatat agtacttatt ttagtgatat taaatttgat ggaatttttt
    10201 tagataaaat aaggtatggt tctttttcga ataaactaaa tggtgttttt aattgttttt
    10261 gtaaagattg tttaaatata tataaaaaaa ataatattga taaagagtcg ttaatgagag
    10321 aaatgaataa tgtgtctaag ggagttatag ataatgaaga tactccattt ggtataacag
    10381 aatacattaa tggaaaatac aaatttaaaa ataaaatatg ggaagatttt tttgaaacaa
    10441 aaaatcaaaa tatatacaag gcattattag agataagtag ttattttaaa agtaagggat
    10501 tgaaaatagg tatagatgct ttttctcctt ttatttctta ctttacaggt caagatttag
    10561 aaaaattaca agaagtagca gattttataa aaccaatgat gtatagaact acaaaagctc
    10621 cagctggttt accatttgaa tttgaaaggt ttattgatga atccatacat aagaataaag
    10681 agattggaaa aagaaatttc ttagatattt taggaattaa ggagtatgaa ggagatgtat
    10741 atcctataga atttgtacta gaggaaatcg attttatgac aagaaatctt aataaagata
    10801 tatatgtagg agttgaaata aataaaaagg aaattatatc tcctgttaca cctaagtaca
    10861 taagtgaaaa tatttcaaat ataaataaga caacagtaaa aggatatgtt ttatcttggg
    10921 atttgttgtc agcaccaaaa gaaaatattg atgaaattat aaaatggttt cggaattagt
    10981 gaggtgataa agtgagtaag atattggaaa tacaacaaat atataaaagt tttggaaagg
    11041 tagatgtttt aaaagggata aattttgagt tagaaaaggg agaagtaaga gctcttttgg
    11101 gagctaatgg agcaggaaaa tctacactta ttaaaatttt aggaggagtt cattctcaaa
    11161 ctaaaggtaa agtaattttg actggtaatg aaataatgtt taaaaatcca catcattcta
    11221 aagaaaatgg tattagtata atttatcaag aattaagctt gataccaacg cttacagtac
    11281 ttgaaaatat ttttcttggt agagaaattt taaaaggaag tgtgttttta aacaagaaag
    11341 acatgcttga ggaatatatg aaaatttgtg aggattttga ttttaatata gacccacata
    11401 tagtagtttc taatttaagt gtagcaaacc aacaaatggt tgagataatg aaagctgttt
    11461 catgtaattc tgaaattatc ataatggatg aacctacaac atcacttact aataatgaaa
    11521 aagatgggct atttaaaata ataagtagac taaagtctaa aggtaaaaca atagtctata
    11581 tttctcacat attagaggaa gtttttaaag tatgtgatag tgcaagtata atgaaaaatg
    11641 gaattatgat tggaacttat gatgtagagg atttaactaa attaaagata actcaactta
    11701 tgacaggaaa tgataatcac aaacttattg agaagaagga ttatacattt gcaaaatatg
    11761 aagagccacc agtactagaa gtaaagaata taagctggtc taacaaaata aaaaatgttt
    11821 catttaaagt ttatagaggc gaagttgtag gtcttgcagg tcttgtaggt tcaagaagaa
    11881 ctgaaattat aaatttgata tttggtatag ataataaaga tagtggagat atttttatca
    11941 atggtaaaaa agtagacata acttctccta aggttgctat aaaaaacaaa ataggattga
    12001 ttcctgaaga tagaaaaaat ctaggattaa ttttagaaca agctatatac acaaactcat
    12061 catcagtaca aattgataaa ttcaagaaat ttttgtttat agatagaaat aaagaaattg
    12121 agttttctaa aaaaggtgtt aaggaattag gaataaaagt tagtgatgta aagcaagaag
    12181 taaaagagct tagtggtgga aatcaacaaa aagtagtagt ttctaagtgg ttagagaaag
    12241 atttagattt aataatatat gatgagccga caaagggaat agatattgct gcaaaagaag
    12301 atattttcaa tacaatcaaa gaattttcaa gtaaaggaat aggagtaata tttatatctt
    12361 ctgatttaga agaagtaata agagtaagtg atagaatttt agtaactaga gaaggttcta
    12421 ttgtagatga tttaaaaaat gagaatatac aggttcaaga tataatgaac agtatattta
    12481 atgtataaaa aatgaaaatg gaggactgag atgaagaaag cttttgagaa gttaaattta
    12541 agagattatg gagtattatt aggtttttta gctttatgta tagctataag tattgcaact
    12601 ccatcatttt taggtaaaca aaatatactt aatcttttac gacaatcttc tataattgga
    12661 ataatttcag caggtatgac ttttgttata atatctggaa actttgatat atcagttgga
    12721 gcagtagcag ctcttgcagg tgctataaca atgaaatttg cgactggtgg aactaatttg
    12781 tttgcatcca tgttcttagg tgtagcgatt tgtgcagtaa taggattaat caatggtata
    12841 gtagttgcaa agataaatgt accatcatta atagccacta tggctatggt cactattgta
    12901 agaggattgc tgttgatgct tacaggagga tatccaatta ctgaaaatat accaatactt
    12961 gattacattg gaaatggata tttgtttaat ataccaatac cagtagtaat tttctttata
    13021 gtggtgttag cttcgtttat tgtacttaat aaaactaagt ttggtagata tgtttattct
    13081 gtaggaggga atcaagaagc aagtaaactt aatggtataa atgtagattc tcacaaagtg
    13141 aaagtgttta ttattaatgc tgtacttgca ggaatagctg gaatcgtact aacaggaaga
    13201 cttgggactg caactgcgat tgcaggtgaa ggatatgata tggatgcaat tgcatctgta
    13261 gtaataggag gtacatcagt ggcaggaggt tctggttctg tacttaaaac tgttatagga
    13321 gtattactta tgagtgttat aaacaacagt tttaatcttc ttggaataga tgtatatttc
    13381 cagtacatat ttaaaggatt aataatttta gcagcagttg gatttgattc ttatagcaag
    13441 aaaaaacttg cttcaagata aaaaataagg gggatttttt tatgaaaaaa ttaatgaaac
    13501 atttagcatt gttgctatca cttgttatga tatttggttt agttggttgt tcaaatggtg
    13561 gtgacaaaga aaaaaatgga gaaaagaaaa tagcagtatt actaccaggt tcaacaggtt
    13621 actttgtagc aactaaacaa ggtatagatg caaaatctaa agaattaggt gtaagcgttg
    13681 aatatgcaga tgctcaatgg gatgctagta aacaattatc acaagctgaa gattttatgg
    13741 ctaaaggggt agacatgatt gcattatgtg gagttgattc tgctgtaagt gagagaatag
    13801 ttaaagctgc taatgattca gacgttccta tagtagcatt tacaaattct ataggaagta
    13861 acccaactgg agaatttaaa ggtttagtta catatatagg acaaaatgag gaagaaacag
    13921 gagcattaac tggaaaaatt gctaaaaact tattaggaga aactggtggg aaagctgtat
    13981 taatagaagg agtaccagga actactccac aagtattaag aaaaaaaggt cttgaaaaag
    14041 aattaaaaga tagtaatata gaaatagttt ataatcaaac ttcaaattgg gagaaagagc
    14101 aagctttaaa agtaacagaa gatttaattc aaaagaaaac tgattttaat gttataatat
    14161 gtcaagatga taattctgct acaggtgcag gtcaagcttt aaaagatgct ggtcttaagg
    14221 ataaagttaa agtcataggt ttgggtggaa gtaaagatgg attaaaagct ataactgatg
    14281 ggttaataga tggtacaact tatatgtctg ctgtagaaga aggtggatta gtcatagaaa
    14341 aggcatctaa attccttaaa ggtgaaaaaa tagaacctgt tacacaaatt aaacaagttg
    14401 aagttaacaa agataatatt tcagagttca agggtgaatg gtaaattgtc atagataata
    14461 aataagattt ttagagttac tttagaaact atataaagta actctttttt taatttaatt
    14521 tagtgataaa ttattgaata gtatttctaa acaaataaaa aattaatata aacaaagact
    14581 taattatact aaattataag taaaactaat ataaacttat ggaaaaaagg aaaacgtcta
    14641 aagtattgga attactatag tgtaggtgta gtattaaaaa aacataaaaa aacataaaaa
    14701 aattgttgaa aaaaatatag aaattttgta taattaaaga tttgaaaagt gcggtataca
    14761 aagccaattc ttttgtatat aatatatata aattcagaaa aaggaaggga tatgtatggc
    14821 caggcggtta tttttagaaa ggattggact tatattttta ggttcagcaa tattagcatt
    14881 tggagtttat aatttttatt atttaaacaa cataacagaa ggtggagtgt taggtatttt
    14941 actactgtta aaaaatttat tcaatataca accagcaata gcaaatgttg taatagatgg
    15001 tttactactt ttagtaggat ataaattttt cggtaaaaaa ttccttatat attcaatagt
    15061 agcttctata actttttcag tgttatatga cttatttgaa gctataggac ctctagtacc
    15121 acaatcacaa aatatgcttt tatcaactat tttggcaggt gtaactgttg gaactggtgt
    15181 aggtatagta gtaaaagcag gttgtgcatc aggaggagac gatgctttag cattagtaat
    15241 ttctaagact acatctttga acatagggca agtttattta gcaacagatg ttattgtatt
    15301 gttgttatca ttattttatc tatcagcgtt tgatatcttt tattctttga ttgctgttac
    15361 aattagtggc aaggtaattg attttattta ttatcatggt aaaagtttgg atatggatat
    15421 aagtaatgat atagttccag aatgttaata taaattaaat aactttagat tttattaggg
    15481 gtggcagatt tgccaccttt tttgtgtcta aatctttata gaaagtataa tcccatctag
    15541 gtttttgcaa atctcaaata tagaattatg ttggaatgac tgggaatacg attgctagat
    15601 gtgcatttta aaaacccaat tttatattta tgaaatacta atcattaatg gatgtttctt
    15661 aatcctatct ttatacctaa aatgataaga taaatgaagt agcaatacaa aactaaaatt
    15721 tgtaaaagag attaaattaa atggaggagc taaaatgttg gatatagtta tgatagcatt
    15781 gttattcata ggatttgttt ccatgaagtt acttgctgat tggtgtggga aacaaactga
    15841 aaaaaaataa tgtaatgtat ggagggatta cctatgtggt ttctggcagt tgtaattata
    15901 atgttaataa tatatttggt atatgcgctt attaatcctg aaaaatttta aacagtataa
    15961 ggctaataag taagttgaat tggggggaac aatttttatg gtgcagatag taatagtgtt
    16021 agcgattttt atgattttag taatacctat gggcaagtat ttatatcata tagctactaa
    16081 tcaaaaaaca tttggagata gactatttga taaagtggac aattttatat ataaggtatg
    16141 tagtatagat aagaaaaaag aaatgaactg gaaacagtat gcattagctt tattatttac
    16201 aaatgcagtt atggtattta taggatatat catacttaga actcaaagta tgcatatttt
    16261 taatccaagt ggaattaaaa gtatggaaca aggactatca tttaatacaa taataagttt
    16321 tatgacaaat acaaatcttc aacattattc aggtgaatca ggactttcat actttagtca
    16381 gatgacagtg ataatatata tgatgtttac atcagctgca acaggatatg cggctgctat
    16441 ggcatttgta agaggtctag tcggtaaaaa gaaaacattg ggaaattttt atgtagattt
    16501 aattaggata acaacaagag tacttttacc aggagcttta attattgggc ttatattagt
    16561 tacacaagga gtaccacaaa cttttgctgg gacagaaaca gtgactacta tagaaggaaa
    16621 gcttcaagat atagctagag gtcctgtggc agctcttgaa tcaataaaac atttaggaac
    16681 aaatggtggt ggattctttg gttcaaattc atcacaccca tttgaaaatc caacaataat
    16741 atcaaatata gtagaaatat tatctatgat gatattacca ggagcttgtg ttgttgcatt
    16801 tggtcacatg ataaagaata aaaaacaagg ttgggttgtg tttggcgcaa tgtcaatcat
    16861 atttttaata ggtctagtag tgtgctttaa agcagaaagt gcaggaaatc ctattttatc
    16921 acaattaggg cttaatcaaa gtatgggaag tatggaaggt aaggaagtta gatttggtat
    16981 agcacaatca tcattattca caacagtgac tacttccttt acgacaggta cagttaataa
    17041 tatgcatgat acactgactc ctttaggagg attagtacca cttttaaata tgatgctaaa
    17101 tgtagtattt ggtggtaaag gcgtaggtct gatgaatatg ttgatgtacg ctataatagc
    17161 agtattctta tgtggattaa tggtaggaag aacaccagag tttttaacta agaaaatcga
    17221 aggaaaagaa atgaagctta ttgctttact tattatatta catccacttt taatactaat
    17281 gttctctggt ctttcagtag caataccagc agggcttgaa ggaatttcaa atcctggatt
    17341 ccatggatta tcacaggtat tgtatgagtt tgcatcttca gcagccaata atggttcagg
    17401 atttgaaggt ctaggagata acactatgtt ttggaatata acaacaggtg tagttatgtt
    17461 ctttggaaga tatgtgtcta ttatagtact actagcaata tcaagtttgt tagcatctaa
    17521 aaaagctgta aatgaaagta taggaacact tagaacggat aattttacat ttacaatagt
    17581 tttagtactt gttgttttaa ttgttggtgc tttaacattt ttcccagcct tagcgcttgg
    17641 acctatttcg gagcatctag tgttgtggca ctaaaaaatt gggagggagt aaagttttat
    17701 ggaaactaaa aaaacaaagt ttattacaaa agatattttc aaaacttcca ttatagaagc
    17761 ttttaaaaaa ttaaatccta aatacatgat gaaaaatcca gttatgtttg ttgtagaaat
    17821 tggatttata gtaacattga tacttacaat agtacctagt ttgtttggtg atgaaggcaa
    17881 taacttaaga gcttataatg ggattgtaac aataatctta tttataacag tcctttttgc
    17941 aaattttgct gaagctgtag ctgaaggaag aggtaaagct caagcagata cacttaagaa
    18001 aactagaaaa gatacagtag caagggttat agatacgtta ggaaatgaaa aaatgataag
    18061 tgcaagtgag ctaaaacttg gtgatattgt tttagtaaat gctggagaag ttattccaaa
    18121 tgatggagaa gttatagagg gtatagcttc tgtggatgag tcagcaataa ctggagaatc
    18181 tgcacctgtc atgagagaat caggtggaga tttcgcatct gttacaggtg gtactacagt
    18241 tgtaagtgac tggttaaaga taagaataac aacaaaacca ggagaatcgt tcttagataa
    18301 aatgatagct cttgtagaag gtgcttcaag acaaaaaacg ccaaatgaga ttgctttaaa
    18361 tactttatta gtagggttaa cacttatatt tatggttgtt ttaatatctc tttatccaat
    18421 ggcaaactat gcaggtgtaa agattccaat ttctactatg atagctcttt tggtatgttt
    18481 aataccaact actataggtg gattgttgtc agctattggt attgcaggta tggatagagt
    18541 aactagattt aatgtaattg caatgtcagg aaaagcagta gaagcttgtg gagacgttga
    18601 tactatgatt ttggataaga caggtacgat tacttatggg aatagacttg catcagaatt
    18661 tatagcagtt ggaaatgcag attcaaaaga tttaataaat tattctgtta tgtgttcatt
    18721 aaaagatgat acaccagaag gaaaatctat agttgattta ggtatgaaat taggttcaac
    18781 aggtaagaca aaagaggcag aggaagctga gtttgtagaa ttttctgcac aaactagaat
    18841 gagtggtatt aacttagctg atggtagagc tataagaaaa ggtgcttatg atagcataat
    18901 aaaaagagta aaagaatctg gtggtacagt gccagaagat ttagaagaaa atgtaaatcg
    18961 agtagctaaa ctaggaggaa cacctttagt agtgtgtgta gataatgaga tatatggaat
    19021 tatctactta aaagatacag taaaatcagg gcttgtagaa agatttgcta gacttcgaga
    19081 gataggaata aagacaataa tgtgtacagg ggataatccc ttgacagcag ctacaattgc
    19141 aagagaagct ggagtagatg gatttattgc agaatgtaaa ccagaagata aaatagatgc
    19201 aataaaaaaa gaacaattag agggtaaaat agttgcaatg acaggtgatg gaacaaatga
    19261 tgctcctgca cttgctcagg cagatgtagg tcttgcaatg aatagtggta caacatctgc
    19321 aaaagaagca gcaaatatgg tagatttaga ttcagaccca actaagatat tggaagttgt
    19381 tgaaatagga aaacagcttt taataactcg tggggctttg acaacattta gtatagcaaa
    19441 tgatgttgca aaatattttg cgattatacc agctatattt actcttgcaa tacctcaaat
    19501 ggagatatta aatgtaatga aactttctac gtcatttagt gcgatattat cagctttaat
    19561 atttaatgct ataattatcc catgtttaat acctcttgca atgaagggag taaaatatag
    19621 acctatgaaa tcacaagcta tgttaatgag aaatatgctt atatttggtc ttggtggagt
    19681 tatagtacca tttttaggaa taaaattaat tgatttattg attacacctt tagtaatggt
    19741 tcttaattta ggttaataaa tggaggatat aaaaatgaaa acattaaagc cagcattact
    19801 tgtaagtatt gtattgttag ttgtttgtgg acttgtatat ccacttgttt tgacaggtgt
    19861 aagtcaagtt gcatttaaag ataaagccaa tggaagtatg atagaagtaa atggtgtaaa
    19921 agtaggttca gaacttattg gtcaaagctt tacagatgcg agattcttta agggaagagt
    19981 atcatcagta aactataata cttatacaaa agaagattta gtaccagata aagatggaaa
    20041 tacatcttat ggaggtgtat catcagggtc ttttaattat ggagctacaa atccagaact
    20101 tcatgataga gtacaaaaag atatagaaaa gtttttaaag gataatccaa ctgtcaaaag
    20161 agaggatata ccaacagatt tacttacagc atcaggttca ggacttgacc ctaatataag
    20221 tccagcctct gcaaaaattc agataccagc tatagcaaaa gcgtctggaa tatcagaaag
    20281 taaattacaa aaaattgtag atgataatac ttccaaaaaa ctttttggag ttttaggtga
    20341 agatagagtt aatgtattaa aagttaatgt agaggtcgca aaaatattag gtcttatata
    20401 gtattttgtg gaattttagc aacaaaatat tacgcaagat ttaatttaaa ggttaaattt
    20461 taaagagtga aaatcgataa tgattttcac ttttttattt gaatattact aatatttgga
    20521 ataaagtgat tgtaagtata aaatccaaaa aaaggttgca ttatcaaaat gataatacta
    20581 taatatattt atcgttatca atttgataat agatttggac atataggagg aaatagtatg
    20641 ttatataata acgcattaga gttgatagga aacacacctg tagtaaggct gaataattta
    20701 ggctgtacta atgggtgttc taacatatat gtaaaattag aaaaagtaaa tccagcagga
    20761 agtataaaag atagagctgt ttatgaaatg ttagagggct tagagcaaag aggagaatta
    20821 aaaaagggag atgtactagt tgaagcaact agtggaaata ctggtattgc attatctatg
    20881 ataggaaaat taaaaggata tgaagtagta atcgtaatgc ccgaaacaat gagcgttgag
    20941 agaagagatt taatgaaggc ttatggagca aatttggtgc ttacagatgg taaagctgga
    21001 atggcaggtt ccatagaaaa agcaaatgaa cttttaaaag aaaatccaaa ctataaaagc
    21061 ctaaaacaat ttgaaaataa agataatcca aatgcacatt ataaaacaac agcagttgaa
    21121 attatgaatg atgtcaaaga tttagatata ttcgtatgtg gagttggaac aggtggtact
    21181 ttaataggaa ctgcaagata tttaaaagaa caaaatccaa atataagagt aattgcactt
    21241 gaaccaaaaa aatctccagc tatatcagaa aacaagactg gacctcataa aatacaagga
    21301 ataggtgcta actttgttcc tgaaaattat gattctagtg tagttgatga agtaatatta
    21361 gtagatgatg aagatgcatt tgatacagtt aaattacttg cagcaaaaga gggaatttta
    21421 gtagggatat cttctggagc aaatatattt ggtgctatag agatggcaaa aaagtatcca
    21481 gataaaaaga tagttacagt agctccagat ggagttgata agtatatgtc tatgggaata
    21541 ttttaataat tttatttgag ggagtgaata ctttgtttaa gaaaataaat aaagatattg
    21601 aatatataat gaagaatgat ccagcagcta ggtctaagat agaagttttt ttactttatc
    21661 catcagttca tgcaatgata atgcatagga tggctcatgc tctttataag aagaagaagc
    21721 tttttacagc aagattaata tcccagatat ccagatttat gactggcata gaaatacatc
    21781 ctggtgctaa aatgggggaa ggtatattga tagaccatgg aatgggtgtt gtaattggag
    21841 agacagctga agtagggaat agggtgacaa tatatcaagg agctacttta ggagctacag
    21901 gaaaagatac tggaaagaga catccaactg ttggtgatga tgtattaatt ggtgctggaa
    21961 caaaaatact aggaccttta aatataggtt caaattcaaa aattggtgca aattctgttg
    22021 tcgttaagga tgtacctaat ggtgcaacag ttgttggtat accagcaaaa atagtaaaaa
    22081 taagaaacct agaacctgta aaaaagaata aaaaagaagt gtcctatgag tatgatgaat
    22141 tagataatgt atattatata taggatttat ataactataa aataaacttt ataaaaatta
    22201 ttttaaaagg tatttttttc aaatttgagg acataataaa aaagaactga atgttacagt
    22261 tttggttcat caatctttga taaacaagat tttaaatttg tagcgtctag gaggatttta
    22321 ttatgaaagc aaatgttaat caagatactt gtataggttg tggattatgt ccatcaatat
    22381 gtccagaagt atttgatatg aaggatgatg ggaaatcaca tgttatagtt gatgaagtac
    22441 caagtgatgc tgaagaatca gcagcagaag ctagagaaag ctgtccagtt gatgcaatag
    22501 atgtgcatta ataaaataaa tgcctataaa aattgaattt tcaattttta taggcatttg
    22561 tcttttattc agttagtatt tcaacacctg tttctgtaac taaaaccata cattcccatt
    22621 gagcagataa tttaccatct tttgtataaa tagtccaatt attttcttca tcaacaaaaa
    22681 tttcgtgagt tccttcattt accataggtt ctattgtgaa tatcatccca ggaaccaata
    22741 gcatctcagt ccctttttta gatacataac ttacaaaagg gtcttcatga aagcttaatc
    22801 caactccatg accacctatt tctctaacaa cagagtatcc atttttcttt gcataagagt
    22861 tgatagcatc acctatatct cctaaaaatc cccatggttt ggcaacttca agaccttttt
    22921 ggacacattc ctttgtaact tgaactaatt tttttgcatt ttcattaaca ttacctatca
    22981 taaacattct tgaagcatct gaatagtatc cattaaaaat agtagaaaca tctacattta
    23041 taatatctcc atcttttaag ataatgtctt tacttggaat accatgacaa acttcattat
    23101 ttactgatgt acatacactt ttaggaaatc cattatagtc tagtggagca gggatagctc
    23161 cattttgtag tgtaaagtca tgaaccaact tatttatatc ttctgtagac attccaattt
    23221 cgattttatc tgaaatataa tccaaaattt ttgtgtttat tacagcactt ttttttattt
    23281 cctcaatttg actttcattt tttataatat catgagttgg tacaatatga cctaacttag
    23341 caaatgaatc tattttatta tccatatcca tatgacattt tttatatttt ttagtacttc
    23401 cacaccaaca gctatcattt ctatttaact tcataacttt tcatccttct tttattattt
    23461 atttaaatta tgggttacat aaactatttt aaaacataac cattataaat attataacat
    23521 aaatactaag aaatccttta tagatagtga tttattcaaa gtataaatta ttatatggtt
    23581 attgttgatg aaaagaggat agttaaatgt attattggat attagcgcac ttaatagttt
    23641 agttcaagtt tttcaagaaa atcatatgtt agaggacaat tttcctttaa agtatttttt
    23701 gaatcttcat caagataata atgtacaaat gattctgcaa aaaattcttc tataaacttg
    23761 cttatatatg catgtgcaac ttgcttaggg tcataaagct tatatctttc ctgagcaaat
    23821 acttccataa attcttcaga atttgatttt ttatttaaaa catttttatc tatagcatgt
    23881 gcagtttcat gtaattctaa attttttgat gaatgtacat ttgcatagct agggttacta
    23941 taacctattt tagccactat agaattattt ctatatatac caggtacatc tttccaagtc
    24001 ttgcctgatc ttacccaaca tggaggtaat tgatatttta aatcactaaa ttctggttca
    24061 tcagttagat ttgaatttat taattttatt tttatatcct tttctttaag tgtctgtaaa
    24121 tatttaatat ttatttttgc aagtctgttt ttcatatttt caacttcatc tttatcataa
    24181 ttcaagtcat caggaagtat aagcatttgt tcaattatat ctatactact gtcttttata
    24241 ttttcagatt cttcatcaaa cttatattga agagtattat tttcatagat agttatattt
    24301 gtattatttt ctatagaata agagtgaata tcctctaaat ctaaaatatt catttgttgt
    24361 aaaatatgtt ttgtttcgcc actccatact actaaagaaa gtgaattaaa attaattagt
    24421 ggattttctt ttgcttttat atcattgctt atatctacat caaatacata ttcaattgag
    24481 tcttctatgt taagaccatc tgtattcttt ttaatatata aatacgcttg ttttaagtct
    24541 atataaatct catctaaaga ttcgtcataa aattctgata aattattagt aaagagttct
    24601 tcacttaaaa gttcattttt atcagttata ctttcaatta aaatattaat ttcttttatc
    24661 atatcgtaaa tacttatttt tctatttgct ttttcactag atacattgca tggcgcaaga
    24721 gcaatgcaat aaaaatcttt ttctttttta taaaaggctt taagtgattt tgataaaagt
    24781 gtaaagtcta cacctatctg taagttgacc ttaagtgagt cattttgtaa taattcagta
    24841 ttatttcttt tagctgtatt taaattgttt tccataatca ttgccccctt tttattaggg
    24901 tatcataatt tttttatatg tgtcaatttg tttttttgaa aattataatt tttttgaaaa
    24961 ttttattttt tataatttgt gaatttttta ttatagaaat aaatatgtta ttattatgtc
    25021 aaaatttagc attagattca tgtagagtat gttttataca tatattataa ttaattcttt
    25081 gaagattgca tattcaaaat acacaccatt ttttttaatt ctataatata atatttatat
    25141 atttaatgtt aggatgtgat ttaaggtgta ttttacatat attataagat gtaaagatga
    25201 ttctctatac actggatata ccagtaatat agttagaaga atgaatgaac ataaattagg
    25261 aattaattca aaatacacaa gagcaaaggg atttgaaaaa ctagaagttt attttgtgac
    25321 caacacaaaa agcaatgcta tgaagttaga atactatata aaaaaattaa ctagaaataa
    25381 aaaattgtct ataataaaaa acccaagtat acttataaat ttaattgata ataaagaaga
    25441 ttatattatt ggtaaggaaa tagaacaatt aacataaata tttatagtaa cattataatg
    25501 ggtgcatatg gtgtaaaaac ttgacaataa atactattgt aatataatga agttataaat
    25561 atagtaaatt aaaatagcta ggggagccaa gagctgagag gtataccgac ccttacacct
    25621 gatctggata ataccagcgt aggaaagctt agtaaaaatt caagtatgta ttatttgtga
    25681 tatatgctat ttctaagcta tacctattgt aggtatagct tttttaatta agttatttaa
    25741 attaaacttt aggaggcaac aatgagtaat tataaaatac caacacttac aatagccggc
    25801 tctgattcat ctggaggagc tgggattcag gctgatttga aaacgtttag tgcaattggt
    25861 acttatggta tgagtgtaat aacagctata acagcacaga atactaaagg agtttttgct
    25921 gtagaggatt taaataagaa aataataaaa aaacaaatag aggcagtctt tgaagatata
    25981 cctccaagag ctgtaaaaat aggaatggtt tcaagtcctg agattatact tgaaatagtt
    26041 gagaatctaa agaaatacaa tccaaaatat ttggttgtgg acccagtaat gatatctaaa
    26101 agtggatatt atctattaaa gccagaggca aaagaaaact taattaagta tttgatacca
    26161 cttgcataca taattacacc aaatatacca gaggcagaag aaataacagg aataaaaata
    26221 cataatgtag atgatatgaa gagagtagga gaagaaatac tacaacttgg acctaaattt
    26281 gtacttatga aaggaggtca tttagatgga gaagcagtgg atattttagt tggtaaaaat
    26341 atatttaaag tttataaaag tgaaagaatt gataaaaaaa atacacatgg aacagggtgc
    26401 acattatctt ctgcaataac atcttatcta gcattaggat atgaaataac agaagcagta
    26461 aatctaagta aaatctatat aacagaagca ataaaaagaa gttttgatat aggtcatgga
    26521 gtaggaccag tacatcattt ttataaattt gagtaataaa aaaatattgg aggtagaata
    26581 tgtataattt aattaaagat gtaaaaaaat taaatccatt ggtaattcat tatactaata
    26641 atgtaactat aaatgattgt gctaatgtaa ctttagctgt aggagcaagt cctcttatga
    26701 gtttttcata tgaggaagta gaagaaatgg taagtgtagc aaactctgta gtaataaata
    26761 taggaactat gaactcaaat atgttggatt tattcctatt agctggaaaa gcagcaaata
    26821 agtacaataa acctgttgtt ttggacccag ttggagtatt tgcaagcaaa gcgagagcag
    26881 aacttacaag tagactttta aatgaagtca aatttagtgt tgttaaaggg aatgtatcag
    26941 aaataaagtt tataggtggc tttaatgtaa ggggtaaagg tgtagattca tttgatgaag
    27001 aagaagattc tactgagata ataagaaaga ttgctgaaaa gcttgaatgt gtagttgtag
    27061 caactggaaa aatagatata ataactaatg gtaaaggtac atataaaata aacaatggaa
    27121 ctgataaatt aaaaggaatt acaggaactg gatgtatgac tgctagcttg attgcaagtt
    27181 ttatggctgt tactgagaat atattagaag cagcaacaat gggtgttctt acaatgtctt
    27241 taagtgggga attagctaat ttaaataatc caccaatagg tacatttaaa gaaaatctta
    27301 tgaatgctat ttatcaaatg gatatagatg ctttaagtaa aaattctaat attgaatttt
    27361 taaattaata aagagtaagg gagacttaaa atgattgata aagaatcttt aaaaaaatgt
    27421 ttaaagttgt atttagtaac tgactctgaa atgcttaaag gtagagactt ttataaatgt
    27481 cttgaagatg caatatcttc tggaataaca actgtacaac ttagagaaaa aaatgcttct
    27541 ggaagagagt ttttaagaaa agctatgaaa cttagagaaa taactaaaag atatggtgtt
    27601 aagtttataa taaatgatag agtggatata gcacttattt gtgatgcaga tggagtacat
    27661 gtaggacaga gtgacattga tgttagagag gttagaaagc ttataggaaa caataaaatt
    27721 ctaggagtat cagcaagaac tttagaagaa gctatttgtg caaaaaatga tggcgcagac
    27781 tatttaggtg tagggtctat attcacaaca tctactaaat tagatgcaaa atcagcttcg
    27841 tttgaaactg ttaaagaaat taaagaaaaa gtagacatgc catttgtact aattggaggt
    27901 ataaatttag acaatataga taaacttaaa tgtttagaaa gtgacgggta tgctataata
    27961 tcagctatat taaaagctga agatatctct aaagaagtgg aaaagtggac attaaaaata
    28021 taaaattggc tctggtagtg aaaaaaatgg aaaaatttga tataatctaa gagtaaaatt
    28081 taagcaatta taaatgataa acttttctac atatactttt taatcaagat atatgtagat
    28141 ttggttaaaa aggagctata aatgattagg atattgttag tgtgtgttgg aggcatgtct
    28201 tctaccctat tggtaaataa aatggagaag gatgcaaaaa aaagaaatat agactgtaaa
    28261 atatgggcag ttggagaagg agatataaaa tcggagcttg ataactttga tattttactt
    28321 cttggaccac aacttagatt tatgcttgat gacgtaaagt ctatagttgg agatagagct
    28381 ccagtatcta taatagatat ggtaaactat ggcacttgta atggtcatgc tgtgttaaat
    28441 tcggttttag aaatattaaa ataaataata aaagtggatt ctatataata gagtccattt
    28501 ttattatttg ttattaatag gcttaaaact tagcttacca tttctttttt taatctccac
    28561 atagcaagtg aaaaacttga acagcacaat attaaaaata gtaggataac aggcattata
    28621 tctccaataa gaatagtctc accaaatatt aagctacgta aagcgttaat aacatgggta
    28681 aatggattga gcatcactgc tacttttaat cctccagata aactttctat agggaaaaga
    28741 gctgaactta aaaagaaaat aggaagtaca attgcagtca taattgtttc ataaactatt
    28801 tcatttggca ataataaact tatagaataa gctagacttg ataaaaagaa ggctgtcata
    28861 aatatcagta ctatcataag taggattcca ccaatgccag attctatcct tactgagaag
    28921 aatatactta ctatacacaa aatagttact tctatgaaag aaactaagat agcttctaat
    28981 aattgaccaa ggacgattga atacctgctg ataggtgcaa ttaagactct atagaaactt
    29041 ccactgttct tcattataaa gtttataata ccaccactac tacaagaaga aaacacaacc
    29101 agtacaatga tacctggtag tataaaagca gtataattat tgatatttat attattcata
    29161 gtttggtttg caatagaact ataaagcaca agccaaagaa aaggttgtaa tatagtaact
    29221 acaaatgaga ttggattttt aaaacgccac ttcatactac gccataagat atttaacatt
    29281 cccattcctc ctttaagctt tttttagaac ttgtaagtgc taaaaataca tcttcgagac
    29341 ttggctctac aatttctatt gcatcaaatt ctatatcatg ttctaaaagc catttattta
    29401 ttaaagtaaa agcagttcta ctatcgttta cacttataaa aattgaattt tctcgtacgc
    29461 tcataaactt tactaaacca gtacttttaa ttgaatcttt atattttttt atatcttcag
    29521 tattatgaaa agttatacgt agtatatttt gtctaatgta actacgtaaa cttgatggtg
    29581 ttccttgtgc taaatccttt ccattcttca taatacaaat attatcactc aattgttcag
    29641 cttcttcaag ataatgagta gttagaaata tagttgtgcc atactcatct ctaattttta
    29701 ataacatatc ccacatagat tttcttgaat cgacatccat accaacagtt ggttcatcta
    29761 aaaataggat tttaggagat gataccatat tcatagctat gtccaatcta cgttttacac
    29821 cccctgaata tgatgaagta ggatatttta agtaactaga taagtcaaac ttatcaatta
    29881 aagagtctat tctttgtttt gcaatctgtg gttctacttt atacatttta ctttgaaata
    29941 ccatattttc cataagggat aaatgttcat caatagagat ttgctgtgct acacaagcaa
    30001 tttgagtacg tatccaagaa ggattatcta ctaaatcttt tccaaacatt gttacatttc
    30061 cagatgtagg tttgtaaaag gtagtcaaaa tatttataag agaagattta ccagcaccat
    30121 taggaccaag taaagaaaaa atttctccag catttacatt aaagcttaga ccatttaaag
    30181 ctttcacacc atttttatat tcttttacta aattgtctat ttcaatcgct aacatatatt
    30241 attcctcctc tttaagtgga aatattatct ctgtaatata gttatctggg tttcctttta
    30301 tcaacatacc tggtccttta ataaatactt ctctccaagg agtttgtatt tttatattat
    30361 tttcatgaat atatttaaat atcatttcat acgtttttgg caaattacta taagagcctg
    30421 tgtgagtaaa aaataaagct tttgttttag gaaattcttt tgtagttata cccatacgat
    30481 ttggagtttc agcagttgga acacataatt ccatttctgc aacaccagtt ttttggtcaa
    30541 catcatagta gcaaaagaat ggtgcaccgt tagactttcc ttttatagct ttaaatacat
    30601 ttggaaaata ctttgttgcc tcagtcatag gtccattata acgcatggta gccactgtga
    30661 ctgattcgat atttttgatt tcaattttgc aattcatttg tttacctcct aatgtgtgtt
    30721 catattctta ctagattttt aagcattaat acatataaga ttttgattag ttgaaaacat
    30781 ataatcttat ctcgtttgct tgttttcaag tctaacataa cataaaatat atatcctctc
    30841 atttgatgct ccattttgta aagttattat ttaagcttta ctttagattt tttagcaaat
    30901 taaaataaag caatttataa aataagtatt tacaaattaa gtgtattaat cgtataatac
    30961 acttaatagg atactagata aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac
    31021 tatcgtatta atacactaga ataggaggaa tcatgaattt tgaattagat aatacaactc
    31081 ccatttattt gcaaatagta aagtatataa agagacaaat agtaacagga gagttgaaac
    31141 caggagagac aataccatca agaagagaaa tggcacttaa tctaaaagta aacttaaata
    31201 cagttcaaag agcatataag gagatgggtg atatgaatat aataaatact tttaaaaatt
    31261 atcaaagcag tgtaacagtt gatgaaaata tattaaaaaa tttaaaatta gagcttataa
    31321 atgaatcact atctgttttt atagaagata tgaaagcaat aaatgttagc aaagaagaag
    31381 ttttaaaaat aatagaagat aagtattagt tttttaacaa atagaaaact tagtttagaa
    31441 agtttattta taagcatatt ggatgtggag gatagtaaat atgatagaaa taaagaatgt
    31501 cagcaaaaca tataaaagga tgcaagggct taaaataaga aagatagaag ctttaaaaaa
    31561 tgtaagtttt aatatagaaa agggaaaaat aactgcatta cttggtataa atggtgttgg
    31621 taaatcaact atgttaaaag caatagcagg acttataaac attgatagtg gagaaattcg
    31681 tatagatgga gaaaaaataa atgaaaaggt atacaataaa ttggcttttg ttccagatgt
    31741 acaatctcac tttagcaata caacaataaa agaaacattt gaatttatgg aaatttttta
    31801 ttcaaagtgg aataaagaaa aatcaaaaga aatgatggac atatttaaat tggatgaaga
    31861 tgaaataatt gataacttgt caaaaggaaa cattgcaaga gtaaaattaa tacttggatt
    31921 ttgccaagac ccagagtaca tacttcttga tgaaccattt acaggaattg atttgtttaa
    31981 gagggaagag tttataggag taatagcgca atatatggag gaaaatcaag ccattattat
    32041 aactactcat gagatagtag agatagaaag tttagttgat gaggttgtaa ttcttgatga
    32101 agggcagatt ataacttcat ttaatgcaga agaattaaga gaaagagaag gtaaatctat
    32161 attagataag atgagggagg tatataagaa tgaataaact tctatatcta ataaatgtag
    32221 atttgagaag aagcaataag ttttatatag cttatatatc atttttttca ttaattacac
    32281 ttagtttaaa tttattcgaa attaataaat ttaaaaattc taaatttatt atgaacttag
    32341 caatagaaga ttttggaggt atattttatg gactagggat acttagtaat cttggtttta
    32401 tccaaagtat attgttcttt gggatattgg gactttttat atactatatt tttatgtgga
    32461 aaagagagtt cttttcaaat aatcaaagta tttatacact tatgatgtta cctcaaaata
    32521 agtttaagat ttgtatatct aaatcaatag ccttgataac aatgatatat ggttttttga
    32581 taagtcagac agcaacatta tttatatgta aatatatatt taatttcatt tttagaaata
    32641 tgcctataat caatatgaac tttgccaaag atttatatta tttaaacttg aagagtatac
    32701 caattgattt tatctctttt atggctattt atgtattttt actacttata gctatttcta
    32761 ttgtaaattg ctgtatatta tttatctttt cctatataaa taaattttat gtaattttaa
    32821 tcactttgct gattatagca ggcttatatt tttcaacaat atatataaat ataatcactt
    32881 tatttagaaa atattataat cttataggat ttcaatttaa caatttagta gcagggttat
    32941 taagtttttt aattataatg tttattctaa atggaatatc ttatttgcta attaaaaaga
//
LOCUS       DAMJZE010000024        31828 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJZE010000024 DAMJZE010000000
VERSION     DAMJZE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.024"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(162..764)
                     /locus_tag="PC541_001063"
     CDS             complement(162..764)
                     /locus_tag="PC541_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2838534.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            complement(944..1762)
                     /locus_tag="PC541_001064"
     CDS             complement(944..1762)
                     /locus_tag="PC541_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2838535.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            complement(1765..2526)
                     /locus_tag="PC541_001065"
     CDS             complement(1765..2526)
                     /locus_tag="PC541_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838536.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            complement(3016..4128)
                     /locus_tag="PC541_001066"
     CDS             complement(3016..4128)
                     /locus_tag="PC541_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2838537.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(4254..5258)
                     /locus_tag="PC541_001067"
     CDS             complement(4254..5258)
                     /locus_tag="PC541_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2838538.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(5546..6394)
                     /locus_tag="PC541_001068"
     CDS             complement(5546..6394)
                     /locus_tag="PC541_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2838539.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(6430..6900)
                     /gene="trmL"
                     /locus_tag="PC541_001069"
     CDS             complement(6430..6900)
                     /gene="trmL"
                     /locus_tag="PC541_001069"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2838540.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(7094..8263)
                     /gene="nth"
                     /locus_tag="PC541_001070"
     CDS             complement(7094..8263)
                     /gene="nth"
                     /locus_tag="PC541_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2838541.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            complement(8461..10698)
                     /locus_tag="PC541_001071"
     CDS             complement(8461..10698)
                     /locus_tag="PC541_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2838542.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(10804..11718)
                     /gene="queG"
                     /locus_tag="PC541_001072"
     CDS             complement(10804..11718)
                     /gene="queG"
                     /locus_tag="PC541_001072"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2838543.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            12740..13144
                     /locus_tag="PC541_001073"
     CDS             12740..13144
                     /locus_tag="PC541_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2838544.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(13401..14396)
                     /locus_tag="PC541_001074"
     CDS             complement(13401..14396)
                     /locus_tag="PC541_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2838545.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14680..15516)
                     /locus_tag="PC541_001075"
     CDS             complement(14680..15516)
                     /locus_tag="PC541_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2838546.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(16004..16567)
                     /locus_tag="PC541_001076"
     CDS             complement(16004..16567)
                     /locus_tag="PC541_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838547.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(16665..17594)
                     /gene="glsA"
                     /locus_tag="PC541_001077"
     CDS             complement(16665..17594)
                     /gene="glsA"
                     /locus_tag="PC541_001077"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2838548.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(18553..20220)
                     /locus_tag="PC541_001078"
     CDS             complement(18553..20220)
                     /locus_tag="PC541_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2838549.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(20294..21049)
                     /locus_tag="PC541_001079"
     CDS             complement(20294..21049)
                     /locus_tag="PC541_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2838550.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(21059..21595)
                     /gene="lepB"
                     /locus_tag="PC541_001080"
     CDS             complement(21059..21595)
                     /gene="lepB"
                     /locus_tag="PC541_001080"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2838551.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(21761..22297)
                     /gene="lepB"
                     /locus_tag="PC541_001081"
     CDS             complement(21761..22297)
                     /gene="lepB"
                     /locus_tag="PC541_001081"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2838552.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(22397..23707)
                     /locus_tag="PC541_001082"
     CDS             complement(22397..23707)
                     /locus_tag="PC541_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2838553.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(23786..24502)
                     /locus_tag="PC541_001083"
     CDS             complement(23786..24502)
                     /locus_tag="PC541_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2838554.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            24747..26018
                     /gene="sleC"
                     /locus_tag="PC541_001084"
     CDS             24747..26018
                     /gene="sleC"
                     /locus_tag="PC541_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2838555.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(26444..27100)
                     /locus_tag="PC541_001085"
     CDS             complement(26444..27100)
                     /locus_tag="PC541_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838556.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(28212..28622)
                     /locus_tag="PC541_001086"
     CDS             complement(28212..28622)
                     /locus_tag="PC541_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838557.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(28838..30220)
                     /locus_tag="PC541_001087"
     CDS             complement(28838..30220)
                     /locus_tag="PC541_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2838558.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(30231..30590)
                     /locus_tag="PC541_001088"
     CDS             complement(30231..30590)
                     /locus_tag="PC541_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2838559.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(30795..31574)
                     /locus_tag="PC541_001089"
     CDS             complement(30795..31574)
                     /locus_tag="PC541_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2838560.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT        10376 a         5807 c         2958 g        12687 t
ORIGIN      
        1 taattcactc aaataggtaa atacaattaa tatctaaatg ctattaataa gtaatttact
       61 caaataggta aatacaatta atatctaaat gttattaata agtaattcac tcaaataggt
      121 aaatataatt agtacttaaa taaatgctac ttctcatata tttaaataaa catagtaatt
      181 aacccttgag ctatacctat taaaaaccct aagacaagcc ccaaaaactc tatatgtttt
      241 aattctttct tagctattct tattattatc tcttccaatt catataaatc tagttcatta
      301 atcttatctt cgaccatttt ttgtatatct atacgttcat ttgctttatt tattatattt
      361 ttactcaatt catctatact ttgcttaact tcagactcaa ttatctctcc tagataatct
      421 tgtatcatgt tttttatacc actcgggata aatgaaactt tctcatgtat tataattttt
      481 acccttgctt ttatgtatct tacaacttct tccttatctt cgtcagtaat gatatttgca
      541 agtatttcgt ccatagaaag aaattcttct tgaattattt ctcctacatt tgctgctatt
      601 tctgctcttc tctttggtat aagacctact atttctatat ttaaaatagg tattttaata
      661 ggctctattg gtctaaatat cagtcttata gcaactacat tcgtaacata acctataaat
      721 ccaccaataa cagctaatat cagtatccta attaaattat ccattataaa attactcctt
      781 atcaattgta tatttgccag ctatattgca ttaatcaagt agatattctt tatacttaat
      841 aactctagtt atactatagc tttatataca attataagtc ttttgtgtcg atttaaaaag
      901 aattttttta attttatatg gatagctttt atatgcttag attctagttt tccataatta
      961 tactaacttt attttcaaaa attgcttcta cagtatctat aatttccttt gagcaattat
     1021 tatttaacaa atccaatatt tctatttttt tgggacatag agttaataag gtacttatta
     1081 aaaagtcctc ataatttaaa ttttctctta ttaccatatt tataatttct tcatcatcta
     1141 tactgtctat cttatttcca tttttatcgt ataaaacaaa tgtattatct tccattatat
     1201 gaactttaag caaatcaatc ttttcttctt gagtatctat aaagtatttt aaaactctta
     1261 taaattcatc ttggtctttc tttattaaat actcctcaac tgcaatatca cctatagcag
     1321 aaatatattt cataaattct ctcattctaa atctaataaa accttctaca tttataaaat
     1381 cattttcttc tatataattt ctcaccctag taaatactgt ttctctcata aaaatctctt
     1441 ttttagcaaa tatgttcagt gaatatatgt atatattatt tttatcttca ggatatgtac
     1501 gcccatagca ttttagaatg tattctttca aaagtttctc tttcattata cttattatca
     1561 tactagttat ttccgttgat atctcatcaa tatttaaagc tctttgttca gctttagtac
     1621 cattaagtat ttttttattt aacacttctt gctttgcaga atctatcact acttctacaa
     1681 tgtcattcgt atatattaat ctatcgttta cattattatt cctcaaaaat ttcatggaag
     1741 aaatattatc cttctcaacc aaagttatcg ctcctttctt gtcatgagag gaactataga
     1801 acctatatct agtaaatttt cacagctaag tgtaactatc tccttgtttt tactttctac
     1861 aaaacttttt atacgttcat agtcaggatg ttttttaaca tctccacaaa aagctagttt
     1921 atccttagaa atcaaccctg tacatccacc tataaatcca taattcatct caaataaatt
     1981 tatgtgacct ttttctatca ataaacactc tatttctgta ttcttcatac ttttccaaat
     2041 acctttatct gatgttatta tagacgttct atctactata cagattgagc atttagaata
     2101 gccctgctct atatttatct ttttaagttt attattttca atataatcta atattgcttt
     2161 gtcagtatac ttaaaattgt gtatagcata gtcaccaact atcgcaatat tgtaaggtat
     2221 atcataaggg tatctattta ataaattagc ttctccctta attatattaa atttatatct
     2281 acttaataac tctttgtatt gattatatac attcggcgca acaattatat ctccttttcc
     2341 taaattacaa atacatatat ccgggtgata ttttatgctt tcatataatt ctttacattc
     2401 tattgttttt attatctcta tatttcttaa ttttaattca ttttccatta gataactaat
     2461 tcttttgtcc accaaagcta gacacaaatc gtcatctagt ataaatggtt ctcttgtaaa
     2521 ttccattttt tactcctttt tctttttttt gataaatttt ttatgtgaat ataaataaag
     2581 tgtgtagcag aatttttctg cccacacact tattgttata attttatata gttattctaa
     2641 gcttaattag tatagctttg tacagttggt tcaaatttaa taaatatatc tttttatcac
     2701 taatctaaac ttaagcagta tattccgtac agttagcaca aatttaataa atatatcttt
     2761 ttattgctaa tctaaactca atcaatatat tttgtacagt tagactaaat ttaataaata
     2821 tatcctttta tcaccaatct aaacttaagc agtatattcc atactgttaa cacaaattta
     2881 ataaatatat ctttttatca ctaatctaaa cttaatcaat atattttgta cagttagcac
     2941 aaatttaata aatatatctt tttattgcta atctaaactt aagcaatata tttttataat
     3001 cttctacatc aataattaat atgtatcttt cactttgtta tactcttttt ttatgtattc
     3061 caaaacatct ggtggtatat ttctactgct ctttgaagtc aagccaagtt ccccatcttt
     3121 tattttaaat tccattactt tacttccctt aaaaccatct tttgcaacgt tctttatcag
     3181 ttcatataca ggttgttcta catcttttat aattgaactt attacattat ctggagccat
     3241 atcgtattgg tctttattaa tagctataac tttaccacca ttttctcttg ctacctgaat
     3301 agctctttta ctatcatctt ctgtagctga aaatacaaca tctctatcag aatctctcat
     3361 ttcctttgca atagttctag ctgattcagg gttttcaaat atatttgaat acttaactag
     3421 taactcagca tctttatttg aagttttaag cccctttcta aatccagatt catacttatc
     3481 tctactagaa ccctcaactc ctcctataaa accgactttc ccagtatcag tcattttacc
     3541 tgcaataagt cctgctaaat atccagtaac attatcttga aatgatacag aaattacatt
     3601 agctggctga actttataag tatagtcaac tattgcaaat tgctgtttgg gatatttagt
     3661 agcagcttct ttaattggct ctgcaaatct ggctccaact gctattataa aatctggttc
     3721 atacttatgc agtttttcta tactctcttt aaaactttta gaagtctttg gtttttcaag
     3781 ttttatccta acatcaaaat cttcgtcagc cctttttaag gctgatagta cattgttatt
     3841 atatgattcg ttatttaagt tttcggtatc taacaatact ccaatagttt ttcttacata
     3901 tgaatcatct tctgtgtcat cttctttatt cttactttta ctatttgatt tatccttttc
     3961 tgctgaagtt agcaaacttt tagactcact ctgtttcgat ttagcactat ctatattaga
     4021 attattctta tttactttat caaacccaaa atcaaaatta catatagcta atataaaaat
     4081 tgtaaaaact actacaaagc aagccatcaa tatccttttt attttcacaa aattcactcc
     4141 ctgatttaga taggtctgac ctacctctgc atatgtcttt ctatatatgt ctattataac
     4201 atccaattca cctcataact aaactcataa gtattcttgg atattatagt agattattta
     4261 ttgttcttat taacatattc taacacatct ggaggaatat tttgagaact attttgggca
     4321 agtccagtac ttccagactt aactgtgttt tctataacct ttccgccttt ataatttcct
     4381 tttacaaaac tttgtatcag attataagta ggattctcaa aatcttttac aatagaactt
     4441 ataacatttt ctggagctaa ctcatgttgg tctttattcg ttgctataac ttttttattt
     4501 tttgccctta ctgcatctat tacagctttt gaatcatctt cagtagtaga aaaaataata
     4561 tccactccat tatccatcat ttttttagct atagattcta ctgatttgct atctttaaaa
     4621 acatctgcat actcaactga aatatccttt atactactat tagaaaattt aacaccctct
     4681 ctaaaacctg attcaaattt atctctacta gaactctttg ttcctcctat aaaaccaacc
     4741 ttatcgcttt cagtcatttt tccagcaatt aatccagcta ggtatccaga tttattatct
     4801 tcataagaaa ttgaagttat attagaaggt tgcttgtcat actcatagtc tataattgca
     4861 aattgttgct ttggatattt cttggctacc atctccaatg gttttgcaaa tctagctcct
     4921 acagctataa ttaaatcgta attgtcatta cataaaatat ctatactatt agaaaaagtt
     4981 gaggaatcat ctgattccac tttgtttgta tctatattaa gtttcttttg agcattgttt
     5041 aatgctaata aagctgaatt attcatagct tcattatttg ttccttcaat atctagtatc
     5101 attgatacag atttttttga attactttta tcgcttatat cacttttatt atctttgcta
     5161 gagctaggat tattacttgg gttattagca cacccaacca acattccagc tatcattgct
     5221 acagttaaca tcaatatcaa tattcttttg aaacgcatta aatttcactc ctcatacatt
     5281 tacgatattt ttatatccta tttactttta catcctattt ataatttttt tatatacaat
     5341 ttacaagtac attttgaaat ttttttaata aatcttacta taaaaaagtg tgcaatagat
     5401 tcttatctat ttacacactt ccctacaaac taaatctaat atatcaacac ttaatttcat
     5461 ccaaaataaa tccaatttaa catctcaaca attaatattc tatcctaatt taataaaaaa
     5521 ataaataatg tcatccaaaa atatattatt tattttttaa gcatatgata ccagtaacac
     5581 caggaccagc atgtgcacct atacacacac caatttggaa aaatctaatt tctttaggtc
     5641 ttaattcatc ttgcataacc ttagataatt gttctttttc cttaaaatca tcactatatc
     5701 ctatatacac aatttggtct gataaatcat caccacaatt tgattttatc aattccatca
     5761 tcttagaaat tacattcttc tttcctctta cttgagatat ttgcgaaact aatccatctc
     5821 ttatttcaag aatcggtttt atatttaata tacttcctat tgctgcctta gtagatgata
     5881 ttctacctcc cttttgtaaa tattccaatg tatctactga aaatataaca aaagccttct
     5941 cttttaattc atccaaaaca ggcaatattt cagcaacaga ctttccttca cttgcaagtt
     6001 ctcctgcttt tattacaagt aaaccagttc ccatacaaag caagttagag tcatatgtat
     6061 atattttacc atctacatca ttctttgcca ttacagcact ttgatacgta cctgtagctg
     6121 tggcagaccc tgctatataa agtatctctc tatcctcatt tgtatatttc tcaaaaattt
     6181 ccttaaattg ggcataagta gcttgagaag tttttggata tacattttct tctctaagcc
     6241 ttttataaaa ttcatctcca gatatatcaa ttctatcctt gtattctctt tcctccagta
     6301 taactgttaa tgaaactata tctatatcat atttatctaa caaatctttt ggcacgtccg
     6361 ataaactgtc acagattatc tttagatttt tcattaagta tgctcctttc tcatttaggc
     6421 ttgttgaaac tacctcatat ttgggtatcc tatttgtctt aaagcctcgt acactacaat
     6481 agcaactgaa tttgataaat ttaatgacct agctttttca acatccaaca taggcactct
     6541 aatacaagta tccatatttg ctttcaacaa aggttctggt aatccctttg tttctttccc
     6601 aaatactata aaacaatttt cttcatattt cacatctgaa tgtgactgtt tagcctttgt
     6661 tgtagaaaaa aagtatttac tatttggata cttttcttgt aattcttcaa agctatcgta
     6721 atattgtata tcagctatat cccaatagtc aagcccacat ctttttaggt gtttatcatc
     6781 caatgaaaaa cccagtggtt ttactaaatg taaagttgaa cctgttgctg cacaggttct
     6841 tattatattt cctgtatttt gaggtatttc tggttctact aatactatat ttaatgacat
     6901 tttaatcctc cctaagtatc gcttttattt atcaaatttt attttataaa cagaataaca
     6961 ataatattat actattatat aaaataatgt taataataat ttttatttca tgtatataat
     7021 agttttttat ctaacatttt cacaagtatt attatatcag aaaatccctc ttgtacaact
     7081 tactttctaa aagttatttc gtttcattaa gttctttata ataattacaa tcttctttta
     7141 ttgggcaact tgcacattct ggatttctag ctttacacat tcttcttcca tgaaaaatta
     7201 atacatgatg tgagtgtgac catctttctt ttggtatagc ttccataagt gcaaattctg
     7261 ttttttgagg atttggctcg tccactattc ctattctatt gctaactcta aatacatgag
     7321 tatctacagc tattgctgga tgattaaaag cgttactaag tacaacccct gccgtttttc
     7381 ttccaacacc aggtaatttt ataagctttt ctaagctatc aggtacttca ccatcatata
     7441 attcacatag ctgttcagat gtatccttta ttttttgaga cttgcttttg taaagaccac
     7501 aactctttat ctctttacta atttcttcaa tgctcaaatt agcaaagtct ctagctgtgt
     7561 tgtacttttt aaacaactct gaagtaactt tattcactct tacatcagta cattgtgctg
     7621 ataaaatagt tgctattaaa agttcaaagg ctgtcccata atttaactca catttagcat
     7681 ctggatacaa tttttctaac tcgtctaaaa tcttatttac gtctttttca tttgctgact
     7741 catttttaat tttagactta cttttagttt ctttacttgt tttttgtgta gccttattag
     7801 aactactctt ctttgttttt ttctccgttt cacctttttt agtacttttt ttacttactt
     7861 tgatactttt tttatcttct tcaatatcca taaaattctc tatatctttc tcatcaatta
     7921 tctcatcttt tttaccaatc attttatcta ttcctttttc atttatctct tccttctcaa
     7981 ttatatcttt cttattcatt ccttttttat ttatctcttc cttctcaatt atatctttct
     8041 tattcattcc ttttttattt atctcttcct tctcaattat atctttctta tctattcctt
     8101 ttttatttat ctcttccttc tcaattatat ctttcttatc tattcctttt ttatttatct
     8161 cttccttctc aattatattt ttcttatcca ttcctttttt atttatctct tcttccaaaa
     8221 ttacatcttt ttcatttatc tcttttagat ttacattttt cataattatc ccccttcaaa
     8281 ataccaattt ataaaacgct atttataaat agtttaatca tatcaacaaa aaaataaaca
     8341 tcaatatata aaacatacaa aataagaatt taatttatat atatttacaa aaaaagcaaa
     8401 aacctattaa agttcttgct ttttattata cataaatacc cattaaattc tataataaac
     8461 ttattctttt gacacagttt gtatcttgct gaaagatatt aatttttctt ttaccaattt
     8521 ctttatttct tcaaatttaa catctgtcaa tatctctata gcctcttcta ctctttcaac
     8581 agtatatatc ttaaattttc catctcgcac tgcatcatta acttcatctg ataaaacgag
     8641 atttctttga ttttttctag gaattatgac gccttgctca ccatttaacc ctttagtttt
     8701 acatatagaa taaaatcctt ctatcttttc acttatacca ccaactactt gtatatcacc
     8761 cttttgattt atagaacctg taacagcaat attttgttta attgatactc cactaagaga
     8821 tgataatagt acatataatt ctgcagaaga tgcactatct ccatctattc caccataatt
     8881 ttgttcaaaa caaacatatg catttagtga caatagtaaa tcctgtgcaa aattctcaga
     8941 aagatagcca cctaatataa gtactccttt gttatgtata gagccactca tatttacttc
     9001 tctttctata tttactatgc ctttacttcc tgggctagtg gtcgctgtaa tccttgaagc
     9061 tctaccaaaa gagtattctc ctgtacttaa gactgacaaa ccatttatca ctcctacttt
     9121 tcttccctct gtttctatta gagtaaatcc attttcaatt gattcatcca tatgtgtttc
     9181 cactctattg attctttttc tcttttcact tattgctttt ttaacatcac actgctctgt
     9241 atactccgaa tttctaaact ctgcataagc acttccttct attacaactt ccattatttt
     9301 attaaactta gttgataatt tttctaaatc accagataat ctagtgctaa atttaataac
     9361 ctcttcaact gctgatttag taaaatgcct taaattattc ttatcacatt ggtatgctat
     9421 aaatcttgct attccttctt cattatcttc atttttatcc atttcactat caaagtctac
     9481 aaatacttta aaatattttt caaaatcttc atcatatcta tataaaatat catatatata
     9541 gtggcttcca atcagtacta atttaacatc caatggcata ttttctggtt taattgctgt
     9601 ttgtgtttcc aatattactt ttttagtctg caaagtcttt ttaagaattt cccaagacat
     9661 tgtatatttt agtaattggt ctgcataaag tactaaataa ccaccattag ccctatgaaa
     9721 tgctcctgat aatagttttg tgaaatcagt ctttatattt ccatttgcat aatcatactc
     9781 tgcctttcca aatagatttg ctggacttgg gtttatctct actacaacag gtgctgattc
     9841 tttattcttt gagcttccat tgtctacaaa taggttaatt ttatatttaa taaaatgctc
     9901 tttatcatac ttatctttta actcctcttc atctaaatag aataaataca tatattctat
     9961 tatatctttc tttatatttt ccagataagt ttgaatctta tcataatttt catatttttc
    10021 acacaatctc ttgatatgag gttctactac aagtttagct atctcttctt caagctctaa
    10081 cactgctttt tcagctattt cttctaagtt tctaatttta tatacgacct gtatcgccat
    10141 attttctagt tctcttttag ctttgtaaaa ctcttcatca gactcatcac tttcatcttc
    10201 ctcttctttc aaagggataa aaactattcc cattttactc tgttttaatt taaatccttt
    10261 ttcttctcca tattttttaa tttgcttcaa aagcatatct ttttctattt catattcatc
    10321 cagaagttta tttttttcta tttcaaaatt ttcactttca aacgcatttt taaaatcttc
    10381 taaaagactt tcaattaatt tctccatatc tttcttaagt tctcttccaa accccttttc
    10441 taattcaact ataataggct ctctagggtt ttcaaaatta tatatataac accaatcttt
    10501 atgcgtattt tttttggatg catactcatt taaaaccttt aaggcataag tacttttacc
    10561 agttccaggt tcaccagcta tatatatgtt gtacgctgga ttatttattt tcaaccctaa
    10621 ttccatagct tctacagctc tttcttgacc caatatataa tctagtggat ttatttcaga
    10681 tgtattatta tatttcaata ttcccccacc tccccatatt taactttaat gatataatat
    10741 gacttttaca caacaagtgt taaagtaaat acacattaaa gtatatgaaa cagactctta
    10801 ttattagtca tcatacttat ctaaaattat ttccatattt ctctttatta tatttcttcc
    10861 tctccagcta aaagctctat ctccatattt ccttttaaat tctttatttg aaatattttg
    10921 tatctcatca taattcaaat aatctattat attttcttta aattccaaaa tatgagttgt
    10981 tggtatttca acattatgtg gacatacttc ttgacaaata tcgcatccaa ataccttttt
    11041 gccattcttt aaaacaactt tttctttctc tgacaaatct cctttttttt gagttatata
    11101 tgataaacat tttttggggt tcatatcata atttcccaat atagcatttc ccggacaata
    11161 ttttacacac tttccacatt taaaacaact tttttctagt ggtttatctt cttcaaattt
    11221 ataattattt actatatacc ctataaatac gtaagaacca tattcatcag ttatgatatt
    11281 attatttatt ccaaaatatc ctattcctga taaataagct aaatacctgt caactaatgg
    11341 tccattatca gcaaataact tgtatttaaa atctgttact gttttagaaa tataatcaca
    11401 aatttgttgt aaaaagtcct ttactacaat gtgataatcc tttccatgac aatattttga
    11461 taaatttgaa tttttattct tttcttgtct atttatatga tatggaaatg cacagactat
    11521 tatagagtta gcatccttca ttatatgtct tgggttaatt ctgttctcaa ttattggttc
    11581 ttccatacca gttaaataac ctttttgttg tctgtcctta agtatctttt ctaaattatc
    11641 atacttatct acccctgcta ctcctacaca gtttaatcct attaaattac aaaatgcttt
    11701 taattcctta ttatccataa aattattcct ttttaatcta attgactttt taaatttatt
    11761 taattttttt agtcttttta accttttcta gctactaact cttttaaata cttaatcttt
    11821 ttagctattc aattttctta cctacttaaa tcttttagat acttcgtctt tttacctatt
    11881 taatttttta cctactaacg cttttagata cttaatcttt ttatctattt agttttttac
    11941 ctactaactc ttttagatac ttcatctttt ttatttacta actcttttag atacttcgtc
    12001 tttttcattt acttatttaa cttttttatc tacttaactc ctttaaataa ttagtctttt
    12061 tatctattta attattttac ctactaactc ttttgaatac ttagtctttt ttatttattt
    12121 aattttttta tctactaacg cttttagata cttaatcttt ttatctattt aattttttta
    12181 tctactaact cctttatata cttagccttt ttcatttact tatttaacta ctaactcttt
    12241 tagatactta gcctttttca tttacttatt taactttttt acctacttaa ctcttttaga
    12301 tacttagcct ttttaatcta ttcaaccttt tttacccact taaccttctt accttttaat
    12361 ataaactcta aaacttcaaa tatcattagt tatattttga cattctttct atagttaagc
    12421 aaaaagattg attttttctt ataattaata tcccatttac attgccattt attaaatctt
    12481 tacttgactt atatggattt ctttttactc tctttactaa actatactta ttaatattat
    12541 gaaggtctta taatagacta atatgaatat gtccatcata tatcaaattt taaaatttgt
    12601 aagcaaaata cttatcttat attactacat ctaaagtgga aaattttaca aaaaagctct
    12661 gtaaaaacac tttaaataac aatttgcttt taaatcatat acagattata ataaatactg
    12721 tataaggatg gtgaaaaatt tgattaaaat aacaattcca ggtagaccta ttagtaaatc
    12781 taattttaag cttcataaca taaatggtca agcatggatg ccttcaaagg gaaaatattc
    12841 taagtatctt gcatacgaaa acatgattgc aggttttata aaccaacagt atcaagggga
    12901 aactgtcgaa gaaaatctaa taacagtctt gaaattattc ttcccaaata agagaatggg
    12961 ggatttacat aattatccaa aaagtatttg tgatggtatt gaaaaaagtg gaataataaa
    13021 aaatgataaa caacttaagc cagtactttt atttgatttt atagataaag ataaccctag
    13081 ggttgaaatt gaactttatc cagtatctaa atacgatata tcctataata tttttgaaaa
    13141 ataaagattt tatgtgttat tcgacattat attgatactc cagtatttga aactatacta
    13201 atacaacaat aataattgtg ttatgtctat actcaaacaa atgtttttaa aaagcattaa
    13261 gtaaatattt aataagtatt aaacaaatat taaatttata taatagctat aactatgtaa
    13321 atttaatatt tgtatataag accacatcta ataaaaagaa atcggagtaa atttaataca
    13381 ccgatttcaa taaataaaat ctatcctagc ttttcaacaa cagtcttaag catttctctt
    13441 ataggaagtt catatggaca tctactctcg caaagcccac agtcaataca ctcagtaggt
    13501 ttaacatcca taacctcata tttttcttta gcccactctt ttaatccata tctagtataa
    13561 taaccttcac ataaaaaact caatggtata tttattccta cagcacaagg catacaatat
    13621 tcgcatcttc tacagaattt tttacctaaa gaatttctta tttcttcaat ttccttatta
    13681 tctttttcat ctagcacaag attttcaagt acagcgacat tttgtctaac ctgttcaaca
    13741 ctctccatac ctggtataac tacatctatg tactctttag atagtatata tttaatagcc
    13801 agtgttgcat tgtctaaagc acctccagct aaaggtttca ttactattat cccaattcct
    13861 ttttcatgag cttttttaaa cacttcatct gcttgtcctt caactatatt ataagggaat
    13921 tgtatagtgt caaattttcc atcttctata gctttttcta tagtatttaa attgtgacta
    13981 gttataccaa tatgttttat cttaccttgc tcctttgcct ctaaaagggc actataagcc
    14041 attttatctt taaaaagatt atcatactcc tcttctttaa cattatggaa ttgatataaa
    14101 tctataaaat cagttttaaa attatttaaa cttatttcaa catctctctt cattgaatca
    14161 taatctctgg acatactttt agtagctaag aaaaatttat ctctttttcc ttcaatggca
    14221 attccgattg cttcctcact tattgtatac cctcttgcac tgtcaataaa gtttattcct
    14281 tgtttttcta actcatttat aactaagtta gtatcatctt gagtaatcct ttgtattgga
    14341 atgcccccaa agccaactct ctttattttc atgttagtat tgcctaaatt tctcatatct
    14401 ttccccacct aacttttaat aattttttat ataatataaa tatgtcacct ttaattatac
    14461 aacaaattat gagaatcttt gcaatgtatt tacatactat tcataatata attatatttt
    14521 atatcagtct attcataata taatcatact ttatacccag ttaatttgta tacaattttc
    14581 gttattccta tccattaatt taaattatca catttcatga ttgtatctcc accctaatta
    14641 tgttgagggt ggagtatttc acatattttt aataaaggtt tatttactag atagtatttc
    14701 ttttatcatt ttaagctctc tcttgtgacc ttcatcatca aaaggagttt ctaagaaaaa
    14761 tggtaaatgt tttagactag gatgttccat aaaatcaatt aatgctttta atcctatctt
    14821 tccttcccct ataggagcat gtctgtcttt tttgtctcca aatggcatca tactatcatt
    14881 tagatggaca gttttcaatc tttctatgcc tataacctta tcaaattctt ccaaaacccc
    14941 atctaaatca ttcactatat cataaccagc tgaaaaaata tgacatgtat caagacacac
    15001 acctattttt tctttatgct caattttgtc tattatactt tttaattgct caaatttaaa
    15061 acctatttca gttccctttc ccgacatagt ttcaagaagt atagttatat tttcatcacc
    15121 ctttatggca ttgttcaatc cttttactat tctatctatg ccaaaatcta caccaccacc
    15181 tacatgactt ccaggatgaa aacacatgta ttcaattcct aatgagtcca tccttcttat
    15241 atcctcatct atcacccttg ttgcaaattc gtatacatca tcctttgtac cacccagatt
    15301 catagtatat ggtgcatgtg caagcagtgg tgcaaaatta ttttcttttc taatttcttg
    15361 aaatttaatt atatcttttt catcaaattc tttagcattt cctcctcttg gatttctact
    15421 aaaaaattga aatgtatttg caccaatata tacagctgtc tcagccgcat tagcaaatcc
    15481 ttttgctatt gaaagatgtg ttcctaattt aatcatatta atctcctttt atttgtacta
    15541 ctacttgtac tcaattttaa ttttgaacta ctccacctta actacattta gccaacatat
    15601 gaagatggag attcctattt gatagtactt tttcactaaa aattcgtaag ctctaaggga
    15661 tgttctatcc caactaatct cattcctctt ttaacatatt tctagcattg atttaattta
    15721 tattaatttt taattttaat atttacactt taattatacc cattttaatc aatttcaaat
    15781 attataatct tacatacttt gtatattatc taagcacttc ttagatagtg tatacatctt
    15841 acactataca tattatttac aaatacaata ggtataaaaa aacaaaaaaa tagagattaa
    15901 aaatcattgt tttacccata ccaaaaacta ttttttgatt attaagtaaa ttttgatgtt
    15961 caatctctaa tattatatct ttaattaatc cataattaat tctctaacta ggacattcct
    16021 cctcaagagt aggaactata accatagcta tatcatactt agaaccttca acattgtgtt
    16081 gtcttttatt taaaatttca tctatctttg acttcatagt gtcactaagt tcaactaatt
    16141 catcatgagt aagagaaata tcatgataat caactatatt ttcattttct aaaacatctt
    16201 cctctgctgc cttataaaaa acttcactca tattttcaaa ttttgatatg tacaattctt
    16261 ctttttcttc cccattgtct aaagaaaaat taagtatcct attccctata accacatttt
    16321 ttgcacttgg ataatagtac ttttccacaa tccccgactt tattttttcc tcaactaatt
    16381 ctaaaatacc aactttgtat aacattttta tatggtagtt tatttttgca tgaggttctt
    16441 ccaataattg agataattgt tttgctgaca atggcgattt gtcaaatgct tttagaatat
    16501 ctattctacg tggatgcgca atagccttta tgcactccaa atctcgtaat accaatacct
    16561 ctttcattga cccctcctaa aaaatttatt taattcatat ttctttacaa tttaattata
    16621 tattaaatta tatgttgttg tcaaaaaaat cctttcttaa tattttaaaa aatatttaat
    16681 ttgagtttat ttgacaactc ctctaaaact ttaactcctg cgacgctgtt accttttttg
    16741 tcaagcgcag gtccataaac accaatcccc atgcgtcccg gcacagaagc cattatacca
    16801 cccccaactc cagattttgc aggaatgcca actttaatag caaattcccc agaagcatca
    16861 tacattccac aagtcatcat aaatgtcttg acaatcctag aaaccatttc actcattaaa
    16921 tgctcaccat tttctatatc tactccataa tttgcaagat ttattccaat tctcgctaaa
    16981 tctacagaat ctatctcaat tgagcattgt ttaaaataaa ggtctagaac atcttctaca
    17041 ttccctctga taaatccatc acttttcaat aagtaagcca tagctctgtt tctatctcca
    17101 gtcatttttt cagacttata gacatcatag tttattccta tattatcatt ttttgctaac
    17161 tttctaaaga aactaagcat tctttcttct ttttcttcta aacaactccc atttataagg
    17221 gaagtggtta ctatagcccc tgcatttatc attggattac agggtttttt tgtgtcatta
    17281 atttcaagtt tcattattga attaaatggg tccccacttg gctccattcc aacgtttgaa
    17341 aaaacgtctt cccaatcatt atccataagt gccatagcaa gcacaattgg ttttgaaata
    17401 ctttgtatag taaactttgt gctacagttt ccagcactat aaagattatt ttcgctatct
    17461 atgatacaaa ttcctaaatc atttcttcta gcgtttttta attctggtat gtaactagcg
    17521 acttgtccat aattagtata cttcttatta gaacttataa tttcttttaa taatgtctca
    17581 tctaacgtat tcatatgcac ctcttatatt atgttatata ttctatttta gcacaagttt
    17641 atactataat aaaacaatgt taaaaaaaat tatactttaa aattattttc tacaagaatc
    17701 aattcatttt tcaaacaatt taaatagttt aaatagctta ataatttaat aatttaacaa
    17761 cctagcaatt tagtaatcta ataatccagt aatttaacaa cctaataatt tagcaatcta
    17821 ataatctaac tatctaataa tccagtaact taacaaccta gtaatttagt aattcaacaa
    17881 tctaacaatt caataattta acaatttaac aacctagtaa tctaactatc taataatcca
    17941 gtaacttaac aacctagtaa tttagtaatt caacaatcta ataattcaat aatttaacaa
    18001 cctagtaatc taactatcta aataatccag taacttaaca acctagtaat ttagtaattc
    18061 aacaacctaa taattcaata atttaacaac ctagtaatct aactatctaa ataatccagt
    18121 aacttaacaa cctagtaatt tagtaattca acaacctaat aattcaataa tttaacaacc
    18181 tagtaattta gcaatccagc aatctaataa tccagtaatt taaattttta taacaccaaa
    18241 aatttctaac ccaacacctt ttactctttt actcttttac tcttttactc ttttactctt
    18301 ttactctttt actcttttac tcttttaaaa aataatcaca taaattaaca aaatattaaa
    18361 caaaaaagta aaatttaaaa taaaaagctt gcttcataca aaagtataaa acaagccttt
    18421 ttatatatca actcaaactt taaaatctgt atcaatttaa ttctccactt tacacttaat
    18481 atacttaaca taaaccaata acactacttg ctatgccatt taatcttcat aaaacaatct
    18541 ataatacaca cattaatcaa ctattttact tcctccaata aattccctaa gaattgaaat
    18601 acctggtatg tcagaagtat cgtccaattc aacaaaatac tgcaaaaact ttagtaatct
    18661 agtagcttca atatgtgcag gattatcttc ctttatctct tccaacaaag gtttcgcaaa
    18721 tcgttttaaa actgccaatt catatacaca agcagagtta aatatagcat ctgcctcctc
    18781 ttggtacggg aatatattct tcttttctcc tcttctaact gatgcccatt gtaaaatagt
    18841 attatcagca ctatatcctc taaaattata gtctctaaca attcttctta tcattctcaa
    18901 atcagcagta gggattcgat tatggtcatc caaatttatt tgagtaaggg cacttatgta
    18961 tatcttaaat ttatcttcat ctggtataga tgatgttaaa ataggattta gcccatgtat
    19021 accttctaat ataattggct gatttgaatc tatctttatc tttttaccag tgtattctct
    19081 cttaccttct ttaaaattga atcttatatc atcaatttct tcaccagcta aaagtttctt
    19141 taagtctaag ttaaacttct ctaaatctat tgcataaatt gattcaaaat cataatttcc
    19201 atattcatct aaaggagtat gttttctatc tataaaataa tcatctaaag aaattgaaat
    19261 aggatttaaa ttatttactc taagatgtat tgataatctg tgagcaaaac ttgtttttcc
    19321 agatgaagat ggagctgcta ttaaaactac tctcttattg ccatccttta tcatatccgc
    19381 tatttgggat aacttctttt catgcaatgc ttcaaatgtc cttattatat ctccatattc
    19441 attgttttct attattttat taagagatac taccttgtca attcccataa gtcttgacca
    19501 ttcttctgct tcataatata cacttgatag cttaggttga ggtacaaatt tcataggaag
    19561 agtcttatct tcttcactag gtcctaaaat tataacgcct ccattataaa gcttaatatc
    19621 aaatgatttt atataaccag ttgatggcaa catataacca taaaaatggt ctatatagtt
    19681 tctacactta tatactttag cactatcata ctctttgtat ttcaaaagct ctgccttttc
    19741 atacatttca ttattttcaa agatagaaat agcttcattt cttgtaactt caattttttc
    19801 tataacataa tcgttgttta ctatctcttt catcttgttt tttatatttt caacatctgc
    19861 ttctttaagc tttctatcta tatgaacttc acaatatagc ccatctgata gtgaatgttc
    19921 tattgtaact ctgctatccc aaaagatttc tctacatgcc attacaaata taaatgacat
    19981 gactctaaag taaactctct ctccatcttc attagtagta tctaaaaaat ttatctcaca
    20041 atccttctta actctacaat ttagctcttt tagtttattg tctacaactg caagtgttat
    20101 ataaccctta tgctcatcac aaaattcttt tgctatatct cccaatctga ttccagggga
    20161 attttcttct aatgaatatt ccttatcttt tccttctatt tttaagttta tttttttcac
    20221 ttcaacatct ccttcctttt gatgaatata aattttaatt aatgaggcat atcaacaaga
    20281 taaagagagg tatttaaccc tctcctacaa tattattcaa tttacttata gtatcttcaa
    20341 tgtcttcgta atttatttct aatactaaat attctgcatc taatgttaag atctctttaa
    20401 tcccctcttc agataaaatt cctcttccta taccaacatg tgtatcattg ataccatcat
    20461 tatcacttaa gtgtatcagt cttattttat cctttaagtt ctttacataa gcatcctcat
    20521 caattaaaca gtgtccagta tcgtaacaaa aacaaatctt agaattttta atcctcttaa
    20581 atatatattc aaaatcataa gatatatttc caatatttga aaaatcccca ccttttgaat
    20641 aagtattctc tatacataca tatgaatttg tatttaattt atctaaaaaa tccacactat
    20701 ttcctaaata tttgtctcta ttctttctaa ctctatttgt cataacatat cccaaatgaa
    20761 ggttaaaata ccttaaatca aatcctttta gttcaaattc tatcttctca ataaaattta
    20821 tccatgaatt tcgtatatac tctatgtttt cgcaagtatt taattccata ggcaaatgta
    20881 tccctataga taatcctaac ttagaaattc tctctttata tttacataac tcactacatt
    20941 cagatatatt gtcaattcca atctctaaat gagttatttg ctttatactc tcacaaatgt
    21001 ttaaagcctc ctcaatatta aacaataagg acgctatacc aactgccata caaatcacct
    21061 actcaaaaat acctatatct gtaaaaggaa atactcttaa aataactttt cctttaatat
    21121 tttcttcatc tactaatccc acttctttat atctactgtc taaactaact tctctattgt
    21181 ctcccatcgc aaatactttt cccttaggaa tcaccatatc aatatcgcct tcagtataat
    21241 tatcatgtat atatgaaact tcatttaata acttaccatt tatataaacc ttagaatcct
    21301 taatttttag atggtcacca ggaacaccta taaccctttt tacaagctct tttttccttc
    21361 cattctcttg taataaatca gtcttaaata tcattatatc tccacatttt ggttctgata
    21421 acttatatgt catcctgttt gctaccaaat aatcatgtga tttaagtgtt gggtacattg
    21481 attctccatt tactaatgtt ggttttataa aacgagttat aataaatgca aaaaaaagtg
    21541 cagtaataat tatttttatc cactctacaa tctcttcttt aatagtttca ttcattcaca
    21601 aattctcctt tatagttata ttatctttat tgataatgtc tcttaaacac tatcttttag
    21661 ataagtctac taaataaggt gttgtctttt ttatatagac aactattgtg tatggtctgg
    21721 tgatttcagc taatcagcga ttgctccaaa ttttatatgt ttagtcaata gttcctatct
    21781 tagaaaatgg atacaatctg actagaacct ttcctaaaat ggtatgctcg tcgaccaatc
    21841 caacctcatc gtatctacta tctaaacttt tttctctatt atctcccatt gcaaataatt
    21901 ttccttctgg aactacgata tcaatatctc catcagtacg attattatgt atataggaag
    21961 tctcatctaa caacttatca tttacatata ccttggagtc ttgtattttt acatggtcac
    22021 caggaacccc gataactctt tttactaaat cttttttctt tccattttcc tctgttaagt
    22081 cggttttgaa tactattata tctttgtatt ttggctctcc taccttgtac gcaattctgt
    22141 taactatcaa ataatcacgt tcaactaatg taggatacat tgattctcct ttgactattg
    22201 ttggcactat aaaacgagtt attgcaaatg ccaaaacaag agctatgaca atcactttta
    22261 tccattctac aacttctttt ttaactgctt cacccactaa aatcgtctcc tctgtgttaa
    22321 atattaagtc aatctacagc aaaataatct accataaacc tacaatataa atattatact
    22381 tcttttttct aatagtttaa aatacttttc taagaccttt aggataatga ttcttaatta
    22441 caccagaaga ttcttttccc caacctaaag gtattccttc aaccgaaacc aaaacccatc
    22501 ctctactatc attagaattt acaacatccc cttttaaata tttaagaatt gattcactct
    22561 gtacactgaa gttttcaaca ttttttacat cttcaacttt taagtaatga gatagtgcat
    22621 gagaaggctc aaatctattc ttctttaaaa cccctaaatg caaaccatat ctcaatactt
    22681 ttagtttttt cgtatctgga tatatttcag gaagtaggta caaattttct cctcttaaat
    22741 aaaatttatt ttttttatta ctataaaaat caacctttaa aaatttcttt tcaaactccc
    22801 tataatcctt tatttcttta tctaaatttt ttatttttaa ttctttgaca ttacattctt
    22861 cttcttctaa tttttttatt ttggcaacaa aatgaccttc tccctttatc ttatgaggcc
    22921 ataatctttc catttcaata agttctgcat gtggatattc actgataaac tcatttataa
    22981 catgctcatt ttcctccctt gaaaaagtac aagttgagta aattaaaatg ccatccttct
    23041 ttaacatttt atatccatct cttataatct ctttttggat ggattgacat tctactacct
    23101 ttgcataact ccaatctgat atggcaacct catccttcct aaacatacct tgcccagaac
    23161 aaggtgcatc tataataacc ttatcaaaat acccattaaa cgtctttctt aagctattag
    23221 aatcggtgtt tgttataata caatttctcg ctccaaatcg ctctaaattt tcccctagag
    23281 ccttaattct actagcatta atttcattag aaaccaataa acctgtatcg tttagcttag
    23341 atagtatata tgtagatttt ccaccaggag cagcacacat atcgagaact ttttcaccct
    23401 ctttaatatc tgcctttggt attacactca ttgcagaagg ctcttgcaaa tagtatgctc
    23461 cagcctcatg cagtggattt ctaccaggtc tgtcaatttt atcatcataa taaaaacctt
    23521 cttctgccca tggtatttga ttcaaatcaa acaactccaa atcaaataat tttttcttgt
    23581 caatttttaa agtatttatt ctaagcccag tagtcttact ttcctcataa ctttttataa
    23641 aatcatcata ttcatctttt agtatttctt caatatcttc taaaaattcc tttggtaatt
    23701 ctgtcaaatc tataatcccc cttcaatctg ctttacttgt gtgtcattta ttatacttta
    23761 attatttatg tctttccttt aaattttatt tcttcttctt tttcctaaga ttaccgaaaa
    23821 acactttttt agagtgattc atatatttct caagtttatc aagatttatc ttccacaaaa
    23881 ttattacata aactataagt aatataatag cccatctata ttctatcatc aatatgaaat
    23941 caaaaactcc atgtaataaa attggaatta acaccgccat aaacaaatac tcacgtcttt
    24001 tatttttatt tccctcaaat ttatacttag aaatatagta gcccatagta attgcaaaca
    24061 ttacatgagc tggtatagag ataactgccc tattaatacc aacttgcagt gctagatttc
    24121 tactttcaga aaaaatataa accatatttt caattgttgc aaatcctaaa gctaaaaata
    24181 cagagtaaat aataccatct aacttttcag taaagtgttt ttctttaatt agggcaggaa
    24241 tcagtatcag agcttttaca ccttcttcta caagtcctgc aactacaaat gatacatata
    24301 ttagttctga atacccttca aatacattaa acttcatcag taaattttct aaaagtataa
    24361 ttataacact tagaaaagca cctattaaaa aaaatttaat tagatggatt aggggttctc
    24421 tacaatatct atctttagac cttatccaaa acatccctat caatatagga attattgcta
    24481 ataaaaataa atctagcttc atagattatt ttcctttcaa aatttttgat ttatttatga
    24541 tttatatcat ctacttattt ttaacttatt cgtgttttaa atacatacta aaatatacat
    24601 taaattattt taaattattt aaatacaaat atactcaaat actcaaaaaa atttaaattt
    24661 atatctatat tatttaacaa atttcaagct atattcatat cttgaaatat aagggaacta
    24721 aattcattta aagaaagggt gatttgatgc aagatggttt cttaacagta agcataattg
    24781 atgctactaa caatagacct attcaaaatg cagtagtaaa catatattca atgtctaatg
    24841 gtagccaaag ctcatcaact ttataccaaa acctaagaag taatgaatct ggtcaagtga
    24901 ctggtttagt attaccagca ccagatgtag attactctct acaaccatct gatgttagac
    24961 cttatagcca gtatatagtg gaagccatag cagatggata tgagacagtt gtcatagaag
    25021 gaacacagtt gttagctaca attgaagcaa gacaaggagt tcccatgtct cctagaacaa
    25081 gaagtaagcg ttctttctca agacaaagtg agttgatttt tgatatagga gaacatactc
    25141 tatatggtac ttatcctcct aagattccag aaagtaactt aaaaccatta ccacctccta
    25201 ctggtttcgt tgtattagat aaccccgtag tgcctgaatt tatagtagtt catgatggtt
    25261 tgccagaaga ttcatcagct ccaaactatt ggattccatt taaagagtac attaaaaaca
    25321 tagcgtcatc tgaaatatat tcaacttggc ctgaacagac aatctatgca aatgtaatag
    25381 ctataatatc atttacttta aatagagtct ttactgaatg gtatagaaat aaaggatata
    25441 actttacaat aacttcaaca acagcatacg accataagtt tataaataat agaaacttat
    25501 ttgaaccaat taacgtagtt gtagatgcta tttttaatac ttttataaaa agacctccta
    25561 catcaagaca gccactatta gcacaatatt gtgatggaca aaaatcccaa tgtcctgacc
    25621 aaatgactca atggggcagt aaagacttag gtgaccaagg atatgactat gagagtatac
    25681 taagatattt ctatggagat gagatagtat ttgaaagagc acctatagta agtggtgtac
    25741 cagtgtcatt ccctggtact acactacaag ttggttcttc tgggcagtat gttagaacca
    25801 tacaaaatca gttaaatgct atatctaatt cttatccagc tgtaccaaaa gttatagaag
    25861 atggtatcta tggaacagat acagaaaacg ctgttaagat attccaagga atctttggtt
    25921 tacctcaaag cggagtagta gattttaaaa catggtatga aatatccaga gtttatgtag
    25981 ctacaactag aatagcttct ttaaatcctt taatttaaag cttgatttag aacataaact
    26041 caaattattg aataaaatta ttaatttaaa ataaaagtgg ctgttatgaa ctaatatata
    26101 ccataagtat tacttataag tattgtagtc ttaacagcca ttttttagtt ataacttgtt
    26161 tttttagaat cttaaaactt taaatggggg attttaagct tcttaaaaac agtgcttagc
    26221 aacttagatt tctttaaaat attatattta tttttaagtt taataaaatg ctattctaac
    26281 gaaaaaccta ctttttattt aaaatctcac ttattattta aaacactatc catcacttag
    26341 aatattactt cttatataaa ttattattta ttataaatac aaataatatt aatttaaaaa
    26401 gttattccta atttaaataa tactactatc ttcatcagtg aacctaaact gcaactttag
    26461 acttattagc taaagccaat attagaccta aagctataga tgcaaatgca gaagcaatta
    26521 ctctatactc agcagggaat aaaaatacaa ttgtatgaca tggtatccag aaaaatggta
    26581 tagtttttaa aacactaaaa ccaaggtaag cattccagtc cacaatattt accacatctt
    26641 taagttttac cttaatcccc tttccaacct catatttttt atctataaaa cagtccataa
    26701 ccttatggaa taaaaataca gttggtgaca ttagtatatt catagttgca ctcttaaata
    26761 gagcatttgc aaaagctatt ccctcaaatg gaagcatacc atccacttgt gcttgaccta
    26821 cacctgatga gtagattgac attccatatg tggtaactac acctattatt ccccaaacta
    26881 ttgctctata aaatactcct actggtattt tccaatcctt atacaaaatt ctaacaccaa
    26941 gtaaatctcc cattgtagct aatacagcaa actttaaaaa acctccaata tatggatgtg
    27001 cacctgttgc ttccataaat acttctctac tacttggtat tgctaagaca gcaataatac
    27061 aaataagaac tccactccat ataaaatcgc cttttttcat atttataaat actatttaat
    27121 atataaatag ttctcctttc tattaaagat ttatttaatt tacaatctat ctacaattat
    27181 ttattaatca cttattatta gatattcatt ttccatattt aaatttattc tatcccttta
    27241 tctatttatt ttcttactac tctatccacc attatctata tatttatttt acgattactc
    27301 tttcacccta accctttaac tatctatttt ttcttactat tcttatccac atcatccata
    27361 tatttatttt acgattactc tttcactcta accctttaac tatctatttt ttctgatatt
    27421 cttatccacc atcatccata tatttatttt acgattactc tttcacccta accctttaac
    27481 tatctatttt tttctggtat tcttatctac tattatctgt atatttattt tatgattact
    27541 cttttaacct accccctata ctttatagta atatgttttt aacctttttc attattccca
    27601 atatgcttta taactacaaa tactctcccc ccttaaaaat aataataaaa ttaatcaata
    27661 aatttataat catattatta ctctatgtat taagcaatta ctatgccaat aaactttgat
    27721 ttttttaaac aaatcttaaa aatcctaaaa tgctaatttt ctttaacttt taatgaatct
    27781 aatagataac atttaatttt cgttatattt ttcagaatat ttctattatt atttttaaga
    27841 aaaaaaaata atgttaacat ttcttaacat acattaaaaa atgttaacat tataaattgg
    27901 gtttaatact tttactttga ctcgatttac ttttattcta cttcttactt tatttttaac
    27961 ttagacttta tttttttatt acttatttga gtcttattta ttttattctc actccatatg
    28021 ttcaattaca tttgtctaaa ttctttgata cttttattac ataattttaa caagcttaag
    28081 tgttatttgg gtagtgcagt tgataattat ttaaataatc atcaactgca tgtttatatc
    28141 attacagtag cttaatgtat ttaatcatac ttacttagga ttttgaaata tcttagagat
    28201 tatatttaac attagtcaat tgctgcataa ttgtaagcaa ttgaaatttg agacccatct
    28261 tcattttcta tagtgatagg cttcataatt tttaatcctt cagtttttat cttttctaat
    28321 tcttgttcca ttttcttaat tgtttcctca aggtcttttt gacttacatc tttgactgca
    28381 ttagggtctt tagcatagtc ttttaattct tgaatatgtt tagcatactc ttcataagta
    28441 taatgctcaa caccatactt ttccaattca gtagggtcag ttattcctga ttttattaga
    28501 gattcaaatt taggtaaagc aatattagat gaatcatttc catttgcaaa tactgctcca
    28561 gttcctccta gtaccattgc agataccatc attgcactta taagggattt ttttattttc
    28621 ataaccaatc ttcctttctt agagatttat ttgggtagtg tagtttaaaa tgactattgc
    28681 aatagttatc aactacactc ttagactatc acagtagtca tgtttatgtc aagccttttt
    28741 aaaaaacttt tcaataaaaa atactatctc aaagcaaaat taattatttt gagatagtat
    28801 tttaattcta aatcttttat attactaaga tttatggtta ttctatccat gttctatatt
    28861 gttctttatt aactgctaac aaattccctt ttcttaatat gacaaagcga ttatcattat
    28921 agccacttcg ctcaattgaa acttctcctg aatcagtata aagtacatcc ttactattac
    28981 tagatttatt tacatacgtt tcttcactat tctcagaatt agttactttg ctcatagatg
    29041 attcttcatg ctctctaaat tcaattacag atacttttgc cgtactaaaa tgagtctctc
    29101 catcactttt agctttacta acaagaatat cagattctac tttgggagaa tataaatcag
    29161 ttgatttact acttattgaa aatccaattt tatcactttg ttcatttttt gttaaaccaa
    29221 aagattcaac ctcattaatc tctttatttt ctgttgcaaa aacaagacct gtcccacaaa
    29281 ctaatacagt tgaaactaaa aaagtaacat aagttgattt tttaaatttc ataatattaa
    29341 ctattctctt ttctattttt gtctttccaa atccactaca tattgtcatt gaaaatggat
    29401 tattctcctc tgctagactt attagtgtgg ttgcataatc agccctatat tcttcaccaa
    29461 gtatactcat aaccttttca tcacaactaa attctatgtc tttatttagt aaaaaaaaca
    29521 tcacccaaac taagggatta aaccaatgga caacaagagc tacaagcata aataacttta
    29581 acagattatc aaagctatta atatgaacca tttcatgagt taaaacaaag tctaaagttt
    29641 gattattagc ataattcatt tgctttggca aaataattcg tggttttaaa ataccagatg
    29701 taagtggtgt cctagtaaat tcagatatgc aaacaataaa tcttctaaaa cggctattct
    29761 tacacatcca ttttttaatg aactcatgat tttttatatg aattgaagtt ttaaaaagat
    29821 taattatttt aaaatatcta aagcataaaa gtatgctaaa cataatagta actataaaag
    29881 acaaaatgcc caacatttcc aacatcataa tattttcaat attagacatt atttcaaatt
    29941 cgtcagaatt gtttttacct gtagagtcac tagctttctt aagagtataa ttttctgttg
    30001 atgtattacc caattccaca tcaaaagtcg attcaattat actataaata ctaaaaccag
    30061 aatttaaact tactggtatt agaagtttaa ataaaacaat caaccacaat aatacaaata
    30121 cctttttagg taatttttta aataaacatg ctcttataaa aataactatt aaaagtaata
    30181 ctccagaagt aaaactcatt tctaacaatt taatgaacat aaatcttacc tcactttaat
    30241 ttttcaacaa tttgtttaag ttttgtaatc tcatcacttg aaagattttc tttattaata
    30301 aaagctgaaa gaaatttttc tgaagaccct ccaaacattt tattaattaa agtttctgtt
    30361 tcctgttttt gtacttcctc tttgcttatt aaagctttac aaacaaaatt cggctcaatt
    30421 ctctctattg caccttttga cactaatttt tttataacgg tatacgtagt gtttctattc
    30481 catcctgttt cttcttttaa aatttttact aactctccag ccttgctttc tcctctctcc
    30541 catagaactt ccattacttt aagttctgaa tcaaataatt ttatctccat ataaaatcgc
    30601 tcctcaaata tagttatcac aatagtcatt actataataa aaaactatca tagtagtcta
    30661 tactatgtca atcatttatt ttttaataat aaaaactgtc tttttaaaaa attcttataa
    30721 aagacagttt tatcaatgtt atactttaat gtttaattaa agccatattt tatttaaata
    30781 tataactatc tatcttagta catagtataa gatttttctt tatctaatag tatatcctct
    30841 acatctgggt tttcttttac taattgtcta taaaaattac aattagtaac acccacatat
    30901 ggaattagcc atagtagacc tattccaaga gtagctattg aaagtatata ccatcctata
    30961 aagctaaagt ttagtacaaa taatttaagt acatttcctt tcatcatatc taaactagca
    31021 cccattgatt taaatacacc catattatcc ttgtcttcac aaattaagaa agtagcttga
    31081 gaaacatata atagcaatat ataaactggt aacataagaa caagtattat aagaactaga
    31141 gttattccag tagaaataat agtccctatt ccaacattat cacttgataa atattcaatt
    31201 gctccactag taagtaaaac tccaaatgat aaaaacgctc ctataattgt gattataaaa
    31261 gaataaatat acacaataat tgattcaaat aaataaaaac caaaacttct taaataaatt
    31321 ctcccactta caaagatatc tcctatcttg actttttcat ctttagtaat tttcaagaac
    31381 atacatgtaa atcctgctga aacgcaaaaa gctattacag ccactacaat agtagtcaat
    31441 ggttcaaaag gtaatcctac aattgatatt aatactatca atgcaatata tactgccata
    31501 gaaattgcac atatccccca gtttccagat aactgagctt tagatactcg ttttaattct
    31561 tgccttgata ccataaaaaa cacccctttc attttataaa aaaactttta aaaatataaa
    31621 taatctttta acgtataata ttaatatatc atattttcga ctatataaga tacaattatt
    31681 caccatgttt tatcaagata tgccataatt gtattactag ttttatatag tcaacattac
    31741 caaatacatc aaataaattt gattaattat tcaacaagta ataaaataat ttctattaat
    31801 tatactttat ttagtaaaca aattattt
//
LOCUS       DAMJZE010000025        30753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJZE010000025 DAMJZE010000000
VERSION     DAMJZE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.025"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            180..2936
                     /locus_tag="PC541_001090"
     CDS             180..2936
                     /locus_tag="PC541_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2838561.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            3068..3547
                     /locus_tag="PC541_001091"
     CDS             3068..3547
                     /locus_tag="PC541_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838562.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            3563..3985
                     /locus_tag="PC541_001092"
     CDS             3563..3985
                     /locus_tag="PC541_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2838563.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            4025..4816
                     /locus_tag="PC541_001093"
     CDS             4025..4816
                     /locus_tag="PC541_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2838564.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            4809..5609
                     /locus_tag="PC541_001094"
     CDS             4809..5609
                     /locus_tag="PC541_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2838565.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            5657..6712
                     /locus_tag="PC541_001095"
     CDS             5657..6712
                     /locus_tag="PC541_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2838566.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(6891..8837)
                     /gene="feoB"
                     /locus_tag="PC541_001096"
     CDS             complement(6891..8837)
                     /gene="feoB"
                     /locus_tag="PC541_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2838567.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(8855..9073)
                     /locus_tag="PC541_001097"
     CDS             complement(8855..9073)
                     /locus_tag="PC541_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2838568.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9279..9788
                     /locus_tag="PC541_001098"
     CDS             9279..9788
                     /locus_tag="PC541_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2838569.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9866..10027
                     /locus_tag="PC541_001099"
     CDS             9866..10027
                     /locus_tag="PC541_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2838570.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            10178..10843
                     /locus_tag="PC541_001100"
     CDS             10178..10843
                     /locus_tag="PC541_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2838571.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            10909..12693
                     /gene="pepF"
                     /locus_tag="PC541_001101"
     CDS             10909..12693
                     /gene="pepF"
                     /locus_tag="PC541_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2838572.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            12945..13787
                     /locus_tag="PC541_001102"
     CDS             12945..13787
                     /locus_tag="PC541_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2838573.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      14020..14103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14683..15360
                     /locus_tag="PC541_001103"
     CDS             14683..15360
                     /locus_tag="PC541_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838574.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            15432..17447
                     /locus_tag="PC541_001104"
     CDS             15432..17447
                     /locus_tag="PC541_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2838575.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            17497..18177
                     /locus_tag="PC541_001105"
     CDS             17497..18177
                     /locus_tag="PC541_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838576.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(18328..19446)
                     /locus_tag="PC541_001106"
     CDS             complement(18328..19446)
                     /locus_tag="PC541_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2838577.1"
                     /translation="MNIKSAFIRKRGEKFHVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFITPNKITLVERCYKYIIANEKNWSPYTTVNRKSWVNNYIEPFF
                     KDIKLIDINPSLLQFFINKSFNNSTSSSAKVRYNFLSSVLKEAYRLKEISENPCDFVK
                     LPAQNVTYEIEIYNREETLLLIEKLKDSLIEIPILLMLLLGLRIGEVAGLRWSDVDLD
                     NSIININQILIYANSKITFKEPKTAKSKRTLSVPKELIEKLKIEKLKQNKMKLQGTLE
                     NENNLVCLNTNLKPWIPTALSKTFHNFIKRNNLRNIRVHDLRHTNASLLLLGGTNMKV
                     VSERLGHTDIKITMNRYSHVLEEMDKEASDNLSKLLFK"
     gene            complement(19960..21624)
                     /locus_tag="PC541_001107"
     CDS             complement(19960..21624)
                     /locus_tag="PC541_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDF2838578.1"
                     /translation="MNNENEQLKSDLLINDEKIKNLNFKIPWYYSLWTISILILSTFS
                     TYSISFIVAIIFLFKRNKIMKKHKDSISILLSDVEKINNKYILLNDEIKMKEKHFEDL
                     CESNENKLKELSNLLDKKKVEIDKFDSENQDKFKLIEELKIEKERLDNLIKDKNILKD
                     NINTLNSHLEELKNEREELRDINTTLKNKKEELKRLSEELIQTEDEVLLQSFGLYNPK
                     YDFENSDEYMEKLKEIREMQKLLIRNKTGVKYSDSWTVDGSVQKGRTMTNQNIKTALK
                     LFNSECDIAMSKVSFKNIDSIEKRIRKAFTDTNKLNTSNKVSIKENYLNLKIDELYLY
                     YEYLQMKEEEKEEQRALREQMKEEALVQKEIENQKRKLKKEELQFKNELLRLKSTIPE
                     DENDKLEWEQKINSIEEKLALLSKDLDDVLNREQNTRAGHVYIISNIGSFGENIYKIG
                     VTRRLDPTERINELSSASVPFKYDIHATIFSEDAPKLESALHKAFDNKRVNKVNNRKE
                     FFKVTLDEIRTEVEKNFDKTVEYTKLAEAQEYRQTLKIQELNNKLA"
     gene            complement(21895..23343)
                     /locus_tag="PC541_001108"
     CDS             complement(21895..23343)
                     /locus_tag="PC541_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HDF2838579.1"
                     /translation="MSILNLFRKNKNKNNVDCNEKSNESKQSYNDSTTLSLECNSNSP
                     HNIEVKNEINKVLNVSCNDTTNLYELEKQAREKSDALYDIHTNSINKFNPQNNILETD
                     TKPLTSIEKSFLKYIIGENIYEPYIATYWTYEYNINYSYLISKFFNMDYLKISNYIED
                     LTKLTVSELKEILKSNNIKSTGKKAELIERIEKEISCKDLSNFFNSSNKYYALTDKGK
                     ELLKDVRKSVTKNTDLEDQCLELIYIDKYEEAYDLICKYESSKNIQRGININWENHKI
                     TPMKIESYKAIKELDINLKDTLLDNIIKSSYILCDMLGNNSKTSILVKRLAGEKIDNI
                     EINNAINSINDVIYNHSTITYNANDSINNKNLHYMDIVKNNKSIPFEVNNKNYNFKIN
                     ENEIYFFNILKIKMYENELKNNFVFDRMSDGAFNVFDTSDNFIGKVKLQGRKKWIMYM
                     KNEFGSEHIYGELIHLIDGIDAWIKYSKEYLK"
     gene            23839..23979
                     /locus_tag="PC541_001109"
     CDS             23839..23979
                     /locus_tag="PC541_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838580.1"
                     /translation="MEKSKKLLIDTGDTLNKLRENNSEKLEKYIELLKEYYKQENKKE
                     RE"
     gene            complement(23994..24359)
                     /locus_tag="PC541_001110"
     CDS             complement(23994..24359)
                     /locus_tag="PC541_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838581.1"
                     /translation="MIGYRIKELRKEKDITQKELATFLGLTPKMISFYEKEERFPPHD
                     IILKLSDFFNVSTDYLLGKVNVKNIDNLSELELIENLNFSDDIKEALKLISELSPSSQ
                     EKMFKIAKVFLEEELNEKK"
     gene            24504..24740
                     /locus_tag="PC541_001111"
     CDS             24504..24740
                     /locus_tag="PC541_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838582.1"
                     /translation="MNKLKDFRIQKGLSRNQLGAELGLTSRYIAFLENGERVPSLNTA
                     VKIAAYFNTSIEYIFLKNNCTKSTFKEKGAEKYE"
     gene            24733..25524
                     /locus_tag="PC541_001112"
     CDS             24733..25524
                     /locus_tag="PC541_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDF2838583.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            25594..25923
                     /locus_tag="PC541_001113"
     CDS             25594..25923
                     /locus_tag="PC541_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2838584.1"
                     /translation="MSVALQFIDTEDLVKELMGREDTVDFIKMFLDREGIKRMELMTI
                     EEFCEYLKISDVTARNMAREAMITKDFIALKIGRKYMIDRISFEEFIMKNAMKDRDVM
                     KKRKGVI"
     gene            26056..26238
                     /locus_tag="PC541_001114"
     CDS             26056..26238
                     /locus_tag="PC541_001114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838585.1"
                     /translation="MEVTKRFLEYKIQALSERIEFKKTIGYKCIADEKELGAYEDILL
                     MLNSEIESIGGVENEE"
     gene            26228..26473
                     /locus_tag="PC541_001115"
     CDS             26228..26473
                     /locus_tag="PC541_001115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838586.1"
                     /translation="MKSRQELIKDIEKYRKAQYLIYLDIVQRAWADRSLTADEQDRIK
                     QEAYAEYKRIERDTEEAEELLMREEFETDRPLAVQIM"
     gene            26556..27065
                     /locus_tag="PC541_001116"
     CDS             26556..27065
                     /locus_tag="PC541_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF2838587.1"
                     /translation="MSTLYELATDLLEIEEGLTETTRNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLDTKKVETFL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYSTT
                     LTTPKVKKE"
     gene            27075..27683
                     /locus_tag="PC541_001117"
     CDS             27075..27683
                     /locus_tag="PC541_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2838588.1"
                     /translation="MENNNIYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            27693..28574
                     /locus_tag="PC541_001118"
     CDS             27693..28574
                     /locus_tag="PC541_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2838589.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLCKRSKNGRDATANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKNKQDKKQSNSKAVNTPNVSKNKFHNFNETFTQYS
                     PDELDDIIKKSQKVKFK"
     gene            28633..28806
                     /locus_tag="PC541_001119"
     CDS             28633..28806
                     /locus_tag="PC541_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDF2838590.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            28822..29238
                     /locus_tag="PC541_001120"
     CDS             28822..29238
                     /locus_tag="PC541_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2838591.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKSDSNNHKEQQEYIPPGLDPQGFQAIDDDDIPF"
     gene            29572..29829
                     /locus_tag="PC541_001121"
     CDS             29572..29829
                     /locus_tag="PC541_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838592.1"
                     /translation="MIIIRSQNKLDLMRVNRVEIDSRYVYAVFEGESNVREIGRYKSE
                     ERAIEVLNRIQEAIIAGTKFDIINKDGVRCNKEKVFEMPVE"
     gene            29843..30208
                     /locus_tag="PC541_001122"
     CDS             29843..30208
                     /locus_tag="PC541_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838593.1"
                     /translation="MLKVERYFSGSVADNIFEDDLTLRNYLALYCCIYGVNKNGELVF
                     PTRGKMLIEFNVDRDRKKRKKASKVKLINVKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIEEE"
     gene            30326..30661
                     /locus_tag="PC541_001123"
     CDS             30326..30661
                     /locus_tag="PC541_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838594.1"
                     /translation="MNILASVILVIGSFIAGRVYEYRLNLNENDEADSKVLLDVFNEI
                     SVLREENKSLKEKLQEKELLFINRLIDFLHDKKICECCIYDCKIDDIEYDCEDGIKKW
                     LDSEELIFE"
BASE COUNT        11960 a         3381 c         5064 g        10348 t
ORIGIN      
        1 tatggaaaga taagaacctt gagattatgt taaaaaatct agattttata cttataaaaa
       61 gtttgatttt acaaacttac aaaagagaat caagatagga atataattat ctttttcata
      121 aatatataaa tgatatacta atattatagt attattgata tttgtattgg aggttgaaca
      181 tgagggatga aattttaagt tttgtaaatg aatgtgtatt atttaacaac tttgatgaac
      241 ttaaaaaaga aagtggcata ataacagaag atatagtaaa aaagtttaat ttaagtagaa
      301 ctcaatcaag tagattatta aatgatttag taaaatctga taatcttata aagataaaca
      361 gtagacctgt tattttttta cctaaaaata aaataaatga tgtagtagga cctacaaaaa
      421 aaagtattta tgaaaattta gatgaactta aaaatgagtg taaattatta aagcaagaga
      481 ctgtgtttaa tagtatcata ggatatgata atagcttaaa agaagtaatc gaacaaataa
      541 aaaccgctgt actttatcca aatggcggac ttacaataat gctgacaggt gaaagtggag
      601 taggaaaaac ttttttagcc aagataatat acaaatatag tgtaagcagt aatgtattag
      661 aaaaaaattc tccatacaat gtgttaaact gtgcgcagta ttataataat ttggagttat
      721 tgtctagtct attatttggg catgtaaaag gagcatatac tggagcaaat gaatctagag
      781 caggtctaat agaacaatca gatggtggtg ttttattttt agatgaagta catagattaa
      841 atagtgaagg tcaggaaaag ttatttacat ttatggatac aggtacgttt tctaggattg
      901 gagagaatgg agtaattaga aaagctaggg taagattaat atttgctaca acagaaagtc
      961 tatctgaatt tttacaaacc tttttaagaa gaatacccat aaatatttat gttcccaata
     1021 tagaagaaag agggactatt gaaaaaagaa agattgtaga acattttatt tatgaagaaa
     1081 gtaaggtact tggtttgcct atagagataa ctcaaattac attaaatgcg attttaaaga
     1141 taaaattcaa ggggaatata ggtgaatgta aaaatgtaat aaaatatgca tgtggacgag
     1201 cttatgcaaa aaatcaaaga agaaatggaa atatttttgt tactcttcaa gaccttccta
     1261 gaaatcttta tatagaaaat gtagatatat ttcaattcca aagtggtaaa agcaaaagca
     1321 ttatcttttc tcctaaacaa tttagtattg aaatgtcaaa tggaagaaat aaaagaaata
     1381 taatctatca aagtttcata aaatgtttag attactttga tagactagaa aaaaatgaaa
     1441 taacaaagga aaaatttagt aaaaagagtt ctaatctaat gattagacta atggacaatt
     1501 tagtcttttc aaataatgat gaaaataata acgtacttat ggagcttata atatcgataa
     1561 tgcaggatat atttagaaat ttagaagtta ataacaatat aaaatatgat gggaataatg
     1621 tattggcatt ttcgtcttat ctttatttga aggacgaatc ttatcaattg tctgatgagt
     1681 ataaagcttt agaaaataaa ttactaatat atttaaaaaa agagagttac aaagaatact
     1741 ctataataag taaaatatca acattaataa cgaatagatt ggatatgtat cttaataatg
     1801 tagatataat cataatgatt atatttatta aatcaacaat tataagaatg aaatttaatc
     1861 aagttaatgc gactattata gcacatggat atgctactgc cagtagtatg gcgaatgtat
     1921 gtaatcggat gttgggtaaa aatatattta cagctattga tatgcctata gatgctacta
     1981 ttgcggatgt atcaaaacaa atgtctagat acctagaggg agataggata aagaatggtt
     2041 taattgtgtt atttgatatg ggctctctta atcttatata tgaagattta aagaataaga
     2101 taaatagccc aatgttattt atagaccaaa tatccactat ttctgcacta gaagtaggaa
     2161 atttattaat gcaaggtaaa aacattgaag agattgcaga aattatggat gaagaaacta
     2221 aacctaatat acaattgtat tatcctcaaa aaaataaaga atatgctata atatcatctt
     2281 gttttacagg gataggtact gctataaaaa tccaacatct actttccgaa agtttaattg
     2341 gaatagttga tgtcaaaata ataccttatg attatgatag tcttgtttct atgggagata
     2401 aagaaccagt ttttgaaatg tatgatgtga tggctatagt aggaactgcc aatccaaatg
     2461 tgaatggggt agattttata ttattagagg atataatatc aggaaaagga gaagatgatg
     2521 tttttagaat atttagcaga gtagtagaaa atgaaaaaat aaagaacata aatgattcta
     2581 ttgtaaagaa tttttcatta attagagtaa tagatagtct aactatttta gattcaaaaa
     2641 agataataga gagaatagaa gaaggaatta atgctataga aaatattcaa aagaaaaaac
     2701 tttcaaatga taaaaagatt tctttatatg ttcacttaag ttgtatgatt gaaagattag
     2761 ttagacaaac tgaaattgaa gagtatactg atatggattt gttaatcaaa aaccatcata
     2821 gtgaagtaaa actaataaaa aatgcattta gtgtgctaga aaagagttat agtgtgcaaa
     2881 taccaatttc agagatagga tatatttata atattatcta tggtctacca cagtaaaata
     2941 caagattaca aaataagtga atgaaattta aaaaagtatg ccagtatttg gcatactttt
     3001 tgcttttata gataaatgag agtataaaca ataaaaattt taatgtatga tggaggattt
     3061 ggggaaaatg gtatctttga tgagaattga tgacagattg atacatggtc aggttgcata
     3121 tggatgggca acatcactag gtataaatgt aatactagtt gcaaatgatg aagcaaaaaa
     3181 tgaccaaatg aaagctatgt cactgaactt agcgaaacct agcaatgtaa ctttatatat
     3241 aaggggggta gaggaatctg gagaaatagt agaaaaattt gcttcttcaa agaaaagcaa
     3301 tgtattagtg ttagtaaaaa ataccaaaga tgctttagtt gtagctaaaa attcaggagg
     3361 agtattaaaa gaaataaatg taggaggatt aagatatgag gaagggaaga agaaacttac
     3421 agatttagtt gctgttgatg atgatgatat aaataacttt aaacaaatag gtgatttagg
     3481 gattgatata gaatttagaa tgttacctag agacaaaaag aaacaactat cagatttaat
     3541 aaaataattt aggagaaaga gaatgaaaaa atttctaatt gcaacacatg gagaattgtc
     3601 cagagagctt gttgacacaa gtaaattaat aattggttca ttaactaata ttgagtattt
     3661 ttgtatgaca acggataaat cagcagatga tgctgagaaa gaaattagaa gtatattatc
     3721 aaataataaa gatagtgaag aatttgttgt cttaacagat gtttttggtg gaagtgtggc
     3781 aaacatatgt acaaacttac ttttacaagg gttcaaattt gaattattga caggagtaaa
     3841 tttaccaatg cttttatcta tacttttatt agttgaggag gatacaaaac taatagttaa
     3901 atcaggatta gaggaagcaa aaaatggaat tgtttatgta aatgagttat tagaaaatca
     3961 aaacaaaatc aaaaatgtaa tgtaatttaa tgtaaattaa actaagttaa aatggaggtt
     4021 caatatgtta gtacaagcta ctatgttagc tattatagca ggagttggta tactagatgg
     4081 gcgtatattt ggtcaatcta tgcttgatag gccaatagtt acaggtatgt tagtaggatt
     4141 agttttagga gacataaaat caggaattat gattggtgca cagcttgaat taatttggat
     4201 gggaattgca ggaataggtg cagctactcc tccagatgta gtaacaggag gtgtattagg
     4261 tacagctttt gctatattat caggtaatgg tgcagaagtt gctttagcag tagcagtacc
     4321 agtagcagtc ttagcacaat ctcttggagt attagttaga ataataaatt cgtacttttc
     4381 tcaaaaggca gttttctatg ctaaacaagc tgattttaag aaagttacca taatgatgtg
     4441 gttgccagta atattattct ttttaagtac atcagttcca acctttttag caattatgat
     4501 tggtgctgat aaagttacag cattgataaa tgcaatacct aaagttatat tagacggact
     4561 gggtgttgca ggaacattgt tgccagcagt aggttttgca ttgcttatgg atatgttact
     4621 ttcaaaaaag atgtctgtat ttttctttat agggtttttg ttagcatctt atggtggatt
     4681 agatataact gctatagctt tatttggagt atgtgtagca tttgtactaa acttttatat
     4741 caataacaaa gaaaataatc aaaatcctca attagcaatc aatactttaa cggaaggaga
     4801 gattgacttt gaataaatta acaaaaaaag aattaagaag tatgttttgg cgttcatttg
     4861 ctcttcaagg tgcatttaat tacgaaagaa tgcaaaactt aggttactgt tattcaatgt
     4921 tacctgcaat aaagaagcta tacaataaga aagaaaatca agcaaaagca atagaaaggc
     4981 atttggagat ttttaataca acgacagtag tagttcctgc tatattaggt ataacagcag
     5041 ctatggaaga agagaatgct aataatccag aatttgatga aagttcaatt agtgctgtta
     5101 aaacagctct tatgggacct cttgcaggaa ttggtgattc attattctgg ggaacattta
     5161 ggattatagc agcaggagta ggagtatcac ttgcaaaaga aggtaatatc tttggaccac
     5221 tattattctt actattatac aatataccag catttgccct tagaatattt ggacttaagt
     5281 atggttatca agtaggggta aattctcttg aaagaataca aagagaggga cttatggaga
     5341 aaataatgtc tatggctaca actgttggtt tattcgttgt aggaggaatg gttgcaacta
     5401 tgttgagtat aactactcct ctgaaattca acttaaatgg tgcagaagta attttacaag
     5461 atatactaga taaaataata cctaatatgt tacctttaac ctttgcattt gtaatatact
     5521 acatgttaaa gagaaaggta agtgtaacaa aattaactat aggcactata gtaacaggaa
     5581 tcgctttaca tgctataggt ttgttataga aattaattat aatttattga atatatagat
     5641 aaggaaggtg tataaaatga caatacaaga ttatatgtta gaaacaccag ttagaatgag
     5701 agaaattata tctaatgcag atagcttatt taatgaagtt aagagaacaa atctaaaaaa
     5761 gattataata acaggttcag gtactagcta tcactcagga gtgcaagtac aaccatattt
     5821 acaaaattta ttagatatag atgttgtgaa aatgtatcca tttatgataa ctgaagatac
     5881 attcaagttt gataatgaaa ataccctagt agtaggtgta tctcaaggtg ggagtagtta
     5941 ttctacatac aacgctatga agttagctga agataagggc tgtaaaatag catctatggc
     6001 aggttgtaag aatgctttaa tagatgagat ttctgattat atattaacag taaattgtgg
     6061 tgaagaaaaa tctggagcta aaacaaaagg atattattgt acaaagttaa acttaatgtt
     6121 attaggtctt caaatagcta gagaaaaagg tataatatct agtgaaaagt acaatgagga
     6181 aataaataag atattagacg caattaatag atttgaagca gtgtataagc tctctaaaca
     6241 atggattgaa agaaacaaag aaaagttagt taattctaaa gaaataagaa taataggtca
     6301 ctctgatata tatggagata cattagaagc agcattaaaa ttattggaaa caatgagaat
     6361 acctgtaact gggtatgagt ttgaagagtt tatacatggt atatataatg ctataaatag
     6421 tgattctact atatttatac tagatacagg aaaagaacct agagtaacta agatgattga
     6481 tgtactttct ggttggacgg aaaatgtatt tgctataggt agagatgtta cagaaaatga
     6541 taaaaattta aagattgata tcacagataa tccttactat caaacattta attttatagt
     6601 acctatacaa ctaatatgtg gggaaatacc tacattaaga ggtgtagacc caagtgttcc
     6661 aaaagatact aggttccaca tgaagttagg tagcaagaaa ctgaataagt aacaaaaaaa
     6721 gaggttgtct caaaatagag ataagcctct tttttactag ataagctctt ttttactgtt
     6781 gaaatgatga aaatatattt gattttctat aaaaaagagt gcctcatgaa ctaaaaagtt
     6841 aattttgaga cacccacttt tatcttattt taaactaaaa tatttatata ttagtgtgca
     6901 ttattttttt tattgctgtc ttccttactt gagcaagaat tagcacttgg acatccagaa
     6961 caaatagaac atccactaca gacatttccg ttttttgtag acttgaattt attaaacaga
     7021 ataaagagtg caaggactat aattattcca cctatcaaag cttctattat tgaattaccc
     7081 ataaatagta ctccacctat acttttaact ataaaagcca ttatccatgc tagtataaat
     7141 tgatatgcaa atgatgtaaa catcatttta ttgccatatt cttttttcat agttgcaagt
     7201 gcagctatac atggagtata taaagcagta aatatcaaga atgtatatgc agtaactcca
     7261 ttaaatattg ttggtaagac aacagttaga ttaccatata gtatatttaa agagtttact
     7321 acgatttctt ttgctccaag accacttaaa atagcaactg aagttctcca atcatcaaaa
     7381 ccaagaggtt tgaatatagg agatataata tgacctagag tagccaagaa gcttacattt
     7441 atatcttctg tatatccaaa aacattgaat gaagaaagtc cccaaattac gactgacatt
     7501 gcaaacatga cagtaacaac tcttatcaag aaaccttttg atttattcca agtattttta
     7561 ataagtgcat ctatagtagg gattctatat tctggtaatt ctaatataaa tggttcagcc
     7621 tctgttttaa aggctgtttt atttaagaat aatgcaacta caattgccat aactataccc
     7681 accaaataca gtgatgtagt tactaaagca gcattatgtg gaaagaatat agctacaaat
     7741 agtgcatata taggtagctt tgctccacaa gtcataagtg gtgcaataag agctgtaact
     7801 cttctatctt tttcgctttc caatgttcta gttgccatta tagctggaga agaacatccc
     7861 atacccataa ctataggtat aaaagcttta ccagatagac caacctttct cataacttta
     7921 tccattaaaa atgctgttct tgacatatat ccactatctt caaacaacga tataccaaag
     7981 aataatgtaa atattaaagg gaagaatggt aaagtaccac caacacctcc aattattcca
     8041 tcaactataa gtgatttaaa ccatgggcta gagttagata acatatcact aacaggtgca
     8101 gaaatataag cctctataag actagcaaat ccctcttgta gtggtcctcc gacccagtca
     8161 aaggtaaatt taaataagag gaacaataca ccaataaata tcggatatgc taatatagga
     8221 ttcaagacta tattgtctat cttatcactg atagatttct tttgtgtata tgtagttttt
     8281 gtacatctcg ataaaatgga ttctaatttt ttatatgttt ctgtttcact tccaaagttt
     8341 ttctgatata taaaagcttt ttgcgtagct cccttaatct cttcttctat cctatcaaca
     8401 ccatttttct tttttgctat aattggaaca acaattactc caagttcttc acttaatttt
     8461 ttggcatcta tattaactcc tttagattct gcaatgtcta acatattaag caatataact
     8521 ataggcttgt taaattgcat aagttgagtt gttaagtaaa ggtttcttga taaattagaa
     8581 gcatcaacta catttactat tacatcaaca tcttcatttt ctaaatatga tttagaaacc
     8641 ttttcttcat ttgagaaagt atccattgca tatataccag gtaaatcgac aacctttatc
     8701 tcttttccaa gaaatccttc ttttttttct atagtaacac caggccagtt acctacatat
     8761 tgatttgaac ctgtaagtag attaaaaaca gtagttttac cgacatttgg gtttcctaat
     8821 aatgctacat taatcacggg tagctcctcc tctattattg taaagaaata tttttagcat
     8881 cagattttct aatcgcaagg tcaaagcctc taaatctaat tataattggg tctccaagtg
     8941 gggcaacctt tttaacttct atttcagtac tatttataca tccaagtgca agcaatcttt
     9001 tagataattt aatatctcca tctatactat taataaatcc tttttctcct atttttaaat
     9061 cgtaaacagt cattattaac acctcctaat gaaattcatt atcattaaaa ttgtaacaag
     9121 tatgatacaa aaagtcaaga tttatagagg taattgcaca ttgaaataac taatatttat
     9181 tctaaataga ttaaaaatat tttagtctaa attaggtgtt tttatatatt ttgtattata
     9241 atgtaagatg atagataaaa tttggaggag ttaacttaat gaaaactaga aaaatgacat
     9301 ttttaggtct tatgataggg tatagtttag ctttatatgt attagagaca tatataccaa
     9361 atcctcttat agttatattt ccaggggcaa aattgggttt gagcaatatt ataactttaa
     9421 tttcattaat gttgttaggt gtcaaagata ctgttataat tgttactatc agggtaattt
     9481 tgtcatcaat atttgcaggc cctttatcat atctactatt tagtatagga ggagcttatc
     9541 tgagtctttt atttatgtat ttagcgagta aaataaaaga tttatcacta atagggataa
     9601 gcattatagg tgcaataggg cataatatag gtcaattaat agttgcaagt ttaatcatag
     9661 aaaatatact tgctataggg tacttaccat ttatgttaat ggcatcatta gtaactggta
     9721 tgtttgtagg cttagtatcc aggtatacaa tacctaaatt aaagcatttt tatagtaagt
     9781 tttattgaca taatattgac aaaatataat attgtttgat aaaatgtata taaaaatata
     9841 caaatttgta atagtggtga ttattatggc gaacaatgaa ttagaagcgt taaaaaatga
     9901 gattgaagca gttagggaag agataaatac atacatagaa tatccagaaa tatttcaaga
     9961 agagttagta gaatcaagta agaaaataga tatattaata aataaataca tgtacctaag
    10021 caaatagccc acagttgtgg gtttttttgt atcaaaaatt aacagatggc tatattttac
    10081 atatattaat aataatatta agatataaaa tagtatttct gatgtgtaaa ttatctgtag
    10141 gacataagtt gtaattaagt caggagggtt ttatattatg attgatatag gaagtaagga
    10201 gatagcaata aggttactat tagcactaat aataggagga ataataggct tagagagaga
    10261 aaaaataaga cagtttgcag gatttaggac acatatacta attgcaatag gttcttgtat
    10321 tgtttcaata acttcgattc aattattctt tgattataat ttgtatacaa atgctgaccc
    10381 agcaagaatg cctgctcaag tattatctgg aattggtttt ttaggtgcag gagcaatttt
    10441 aaaaaataaa ggtggagtaa aagggcttac tacagctgct gggatgtggg caactgcatg
    10501 tattggtata gcagttggat atggttatta tgaattggca attctaggtg gattatttgt
    10561 aatgatagca ttatttgtgc ttaggataat caatacgttt ttatttagcc aagaaaagaa
    10621 tgtattgata ttaaaattaa gcaatttgga ttctatacct tttttatatg ataaatttga
    10681 aaaaaatcaa cttatcgtaa gaaatatgga aatagaatgt cgtaatgaag aatactggaa
    10741 ggttaatttt tttgtatctt atgctaagag aatacactta cacgacgtta tgaaagaaat
    10801 aaatatgata gaaggagtaa taaatgtaga ttatttggac taaaatatat acaatatatt
    10861 ttgtaaagtg aataaaatat aaaacaaatt aaaatagagg tgtatgatat gggaactgat
    10921 agaagtacaa tagaagaaaa gtacaaatgg aagatagata agatgtactc cagtaaagag
    10981 gaaattgaaa aagacataag taaagtaaaa aatcttatcc aagaagtaaa agaatataaa
    11041 gggaagcttt ctgagagtaa ggagaactta tataaagtat taaatatctc tgaaaatgca
    11101 tctaggattg ttcaaaattt atatgtatat actcatatga aacaacatga agatactaga
    11161 attaatgata atcaaggaag agccactaag acagaaatgt tatctactga tttaggtgtg
    11221 gctacttctt atatagttcc agaaatactg gcgatagaag agaataagtt aagtgaatac
    11281 ttaaaagatg agaagctatc cttttatgca aaatatataa aggatatatt gagagataaa
    11341 ccacatactt tgagtgaacg agaggaagaa atacttgcag ctacatcaga attatcgaca
    11401 attccagaaa atgtatacga tatgttagca tatgcagata tggaatttcc tgagatagaa
    11461 gatgaggagg ggaataaggt taagctttct cattcaaact actctttatt tataaaaagt
    11521 aagaatagta gagttagaaa agacgctttt gaaggtgaat tttcaacata tgagaaatat
    11581 aaaaatacat ttgcctcaac gctttatggt ggaataaaat cagagatatt ttacgcaaaa
    11641 actagaaagt ataattcagc aatagaagcg tctttatttt cagatgatgt aagtttagat
    11701 gtttacaata atcttatatc agctatagat aaaagtattc ctaatctaaa taaatatgtt
    11761 gagcttaaaa agaaattttt aggattgaat gaaatacaca tgtatgattt gtatgttcct
    11821 ttgacagata aatttgacat ggatatacca tatgataaag cacaggatat catattagag
    11881 gcacttaaac ctttaggaga agaatacttg aaggtcataa aaagtgcttt tgatgaaggg
    11941 tggattgatg tatatgataa tgaaggtaaa aaaggaggag cttattcttg gggaagttat
    12001 gattcacatc cttatatatt aatgagctat aaaaatgacc ttaattctct attcacttta
    12061 attcatgaat tagggcactc tgttcacagt cattattcaa gaacaagtca accattttta
    12121 tattcggact ataagatatt cgttgcagaa gttgcatcta cattaaatga attacttctt
    12181 ataaattatt tattagaaaa ttctaagtct aaggatgaaa ctatatactt attaaattat
    12241 tatttagaac aatttagaac tacagttcat agacaaacta tgtttgctga atttgaaaaa
    12301 attgtacatc aaagggtaga gagtggagaa ccacttactg ctgatgaatt tactaatata
    12361 tactacaaat taaatgaaaa gtattacgga aaatctgcaa tagtagataa acaaatagga
    12421 atagaatggg cgagaatacc tcatttttat tcaaacttct atgtatataa gtatgccaca
    12481 gggttttctg ctgcaagtgc tttaagtcaa cagatacttt cagaaggaag tactgcagta
    12541 gatagatata taaacttctt aaaaagtgga gggtcagaat acccactaga acaattaaaa
    12601 tctgctggtg tagatatgac gaaaaaagaa tctatagagg aagccttaaa tgtatttgct
    12661 gaattagtta ataaactaga aaaagaatta taggatgtga taggttgata gcttcaatga
    12721 agacttctga taatatgagt tgtattatgt ttatatctgc ctaaagtaac tttaaaaagt
    12781 taatctaatt aagaataaat catataaaat taagttttta catgtattta acaatattta
    12841 catttactta accctgctct aatattcatt taacaaagaa tctgtattat taaaatatag
    12901 aaaaatgaca acaaaaaatt aaaatataag cgggggtaag taaaatgttt aaaaagaaaa
    12961 taggcgttct tttagctagt acaatgttag ttggagcttt agcagtagga tgtagttcta
    13021 actcaggagg ttcaggtgat tcaggagatt caagcaaagt ttctgtttca ggttcaactt
    13081 caataggtcc tcttatggaa actatcggag aaaagtttca agaaaaaaat caaggtgtta
    13141 gtgtagaagt acaacagatt ggttcttctg gaggtataaa aaatgctata gaaggaacat
    13201 ctgaaatagg aatgagttca agagatttaa aagatgaaga aaaaagtgct ggattaaagg
    13261 aaactaaaat agcaatagat ggtatagcat tgataactaa tactgcaaac tcagtaaaaa
    13321 atctgacaat ggaccaagtt aaaggtatat atacaggaaa gattactaac tggaaagaag
    13381 taggaggaaa tgatgcacca atagtaatag tatcaagaga agaaggttca ggaactagag
    13441 atggattcca agaaattgta gggtttgatt cagaagaact aactaaagat gcacaaataa
    13501 gtgatggttc aggaaatata aaaactacag ttgaaggaaa tgaaaatgct ataggatata
    13561 tatcatttgg atatgttgat gagaaagtaa atagtttaaa aataaatgat gttgaagcaa
    13621 ctgatgcaaa tgtaatagct aataaatatc caatatcaag accatttata ttagttaata
    13681 aagaagatgg tttgtcagaa aaaggtaaag ctttaataga ttatatatta agtgatgaag
    13741 gacaagcact tgtttcagaa aaaggattta ttaaagttaa tcaataacaa gagttataat
    13801 ataaagtaaa catataatac cctgtgagat aagctcataa aaatgatgct ttaacttgca
    13861 gggtattgtc tattattgaa tgtagaaata taaatatctt tagataaaat aggtatttta
    13921 gtatcttatc tctaagaata tggaaatatt gacaaaataa tattacaatg ttagaataaa
    13981 aaagaaattt atgaaaatat ataaaagatt tttaataaaa atattttaga aactgagaag
    14041 tatatcttat tattgggcat ctagagatat atggagttag tggtgcaacc ggctatgaat
    14101 ataattttta tattgcatag tctttttttg ttttaaaatt aacaaatatg ttgcaaaatt
    14161 tgaaaataag agtattagtc gttaagattt ttattgatag gtaaaatttt ggcttttaaa
    14221 gtagccattt gttggaaaag ggaaattttt ttaaaagaat aatagtatta taataagaaa
    14281 ataaagtttt tataaacatt gagatgcaga atataaacct tttgtaacaa tatatttacc
    14341 aaaataatac aatttatttt atattataac catataaatc atataaatca atactcaaac
    14401 tagccaatag acaagtttct agaaaaataa taaatttata cgtgaaaatg actttaagct
    14461 ccattctcca acaatcggag actttaatta gtatttaata atgaatctta atgtttaata
    14521 caatattcaa gaattattca aaaatactta agtattatag tctagtttgt aaagatatta
    14581 taaaataata ataccagtta tatatgaaaa taaaacatta taatttgtaa taactataaa
    14641 acttggaatt ataattaaaa tacctttgaa tggagtggtg taatgtataa tttactggtt
    14701 gtagatgatg atgttgatat tttaaacata aatcaaattt atttttctaa tataggtttt
    14761 aatgtatata catcagagac tgctaaagat gctatggata tagtagagag tatagtttta
    14821 gactgtataa ttttggatat ttcattgcca gatatagatg gatataaaat gtgcaatgat
    14881 ataaaagaaa aggttaacat acctataata tttttatcta attatgagca tgaagaagaa
    14941 aaagttcggg gatttttagc tgggggggat gattatataa caaaacctta tagtttaaaa
    15001 gaacttgaac ttagaatata tgctcgtatg aggcagtaca aaaatattac atcagttcct
    15061 aatgtgttaa agttttcagc gttagtaata aatgtaaatt caagaaaagt aacttttgat
    15121 ggagaaagta tagatttaac tactatggaa tttgaaatat tattgtttct agctgaaaac
    15181 aaagaacaag tattttcgaa gatagagata tataaccaag tttggaaaat gcctgatatt
    15241 ggagatcaac atacagtgca ggttcatatt gcacagatga gaaaaaaaat taattcttta
    15301 agcagggagc atcaatacat tcaaaccgta tggggaaaag gatataagtt tgtaccataa
    15361 gtgagatact gtaattaata aatagttctt gttttattta atactactat gaaagagatg
    15421 tggttgatac aatgaaaaag tataatatac taaaaggaac tctcattgta ttgatagtct
    15481 ctctgttggc aataacagga ttaaatcttc tgtataaata tgataataaa tatacatata
    15541 agtctattag agcaagtaat ggaattttaa ctatagataa tgatactttc actaaaggta
    15601 aacttgtctt tttgatagat gactgggagt tttataatca taaaatattt aaaccgaatg
    15661 attttacaga gcaagatata aaaccagaat atgtttttat aggtcaatat ccagattttt
    15721 ctatgagaaa ggggactcag tcaccatatg ggaatgctac ttatcgtatg agaataaaaa
    15781 atgaaggcaa ggataagcta ctaagtttag agcttcctga gattttttcc gcaagtgaag
    15841 tatggataaa tggagaaatg gtttctaaat taggagatgt aggaacagaa aaatatagac
    15901 ccaaaattaa aaattcagta gtaagtttta ttgcaaagaa aaatacagaa attataatca
    15961 atgtatcgaa tttttcacat tattatagtg gtttgtatta tccgcctgca ttaggagaaa
    16021 caaaggacat aagtaatatg atgttttatc gattactttt ttatagtata atttgcttca
    16081 caacttttgc aatagctata ttttcattgt ctgtatggtt tctttcaaac aaaagtaaaa
    16141 tttacttata ttttggatgt atgtgtgttt tctttgccat ccatgtatcc tatccctttg
    16201 tacatttgtt aggactgcct ttggtaagct ttacatatgc ggttgaagat ttgtcatatt
    16261 ttatggtagt tttatgtatt atttgtataa atggaagtat atccaaaata gaagaaaata
    16321 aagtgtacag atttttaatt ttaccattag gaattacaat gagtatcata tctatagtga
    16381 taccaattat tcttcttcca tatgatatgt actttgtcaa catatatggg aagttaatag
    16441 acatatataa atatattgta tttatatata tattaatgtc ttcgctggtt tcaatatata
    16501 aaaagacatg ctctgagcat ataagttctt atattttagt ttcagtaaac actgtttttg
    16561 gaattagcat attatttgat gcagttacga gtaatagatt tgagcctata tttacagggt
    16621 ggcaaacaga atattgtggg tttgtaattg ttattttatt ttctgttatg atgataaaca
    16681 ggaatagaat aataataaaa gagaatgaaa aacttacaag gaatcttgaa catgaagttg
    16741 aagtgagaac taatgaactt acaacattgt taaatgaacg taaaagattc ttggcagatg
    16801 tagcacatga tttaaaggca cctgtttcgg ctatacaagc atttatagat ttgataaagg
    16861 ttggtaatat tcatgtagat gaagagacaa gaacctatct ttatgctata aatcaaaagt
    16921 caaatgaagt acaaaataga gtacgtagtt tgcaagaatt tacatcacaa gataaaagca
    16981 ttggtattag tgaaaaaata tgtctgaata attttattaa agagatatat gaaaataatt
    17041 tgccagatac acaagcatgt ggtgtaaact ttgaaactta cgtattggag aaaaaaatat
    17101 atgcaaagat aaataaagag atgcttacaa gagcatttga gaatttaatt tataatgctt
    17161 tgacctttac tccatttgat ggaaagataa ttttatcaat ggataaagaa gatggatttg
    17221 caattattaa aatttctgat aatggaaaag gtatacctcc agaaaatata ccaaaggtat
    17281 ttgacagatt ctttacagaa agagacaata atgagccaaa agggcaaggg ttagggctat
    17341 atattgtcaa atccattata agagagcatg gtggagaaat ctttgtagat tcagaactag
    17401 gaaaaggaac tgtttttaca ataaaattaa gtctatattt tttataataa tagcttattt
    17461 aatataaaag aataatgata tttgggagtg attataatgc aaactaagat attgataata
    17521 gatggagaca aggataattg caagaagcta aaaggatttc tggaggaaaa aggaataagt
    17581 atagatttag catataattg tgaagaagct attggaaaaa tatttagcaa taaatatgat
    17641 ttaatatttt tagaaataat tctaagtgat ggtgatggat ggactctttg taaaaaaatt
    17701 agaaatgtaa ctacttgtcc aattgtttat atgacttata taaatgaaga ccaaagcatt
    17761 ttaaatgctt taaattctgg tggagacgat tacttaataa agcctctaaa tttagaaata
    17821 ttatacgcaa aagtaaaagc tattttaaga agaatgaact catatgtaaa taataatgaa
    17881 aataatacaa aattagagga atataataga actagtggag taataaagtt agaagataaa
    17941 ataataaaat tgactccaac agaaaataag ttgttaaact tttttataga gaatcctgaa
    18001 aagactttga ctactaaaga gatatatgaa catgtatgga tgaatgaata cttagaggat
    18061 aactatagtg tggtagttgc agtgaatgga ttaagaaaga aaatagagaa agattataaa
    18121 aatcctcaga aaattataac aataagagga gcaggatatt attttaataa agaataataa
    18181 atatatataa acaaacataa ataagtatta aaacgtgcag ttttagcggt atttaaaaga
    18241 aattattata aactaactga ctgacattta actgacaaaa cctatttttt taggttcaac
    18301 tgacatttaa ctgacagtca gtttttatta tttaaataga agtttactta aattatcaga
    18361 agcttctttg tccatttcct ctaaaacatg agagtaccta ttcatagtta tttttatatc
    18421 tgtatgacct aatctttctg aaacaacttt catatttgta cctcctagca aaagcaaact
    18481 tgcgtttgta tgtcttaaat catgtactct aatatttctt aaattatttc ttttaataaa
    18541 gttatgaaaa gttttactta atgcagttgg tatccaaggt tttaaatttg tatttaaaca
    18601 tactaaatta ttttcatttt caagtgtacc ttgtaatttc attttgtttt gttttaattt
    18661 ttctattttt agtttttcaa ttaattcttt tggaactgac aacgttcttt ttgattttgc
    18721 agtttttggt tctttaaaag ttattttact atttgcatat atgagaattt ggttaatatt
    18781 tattatacta ttatctaaat caacatcaga ccatctaaga ccagctacct ctccaattct
    18841 taaacctaac agcaacatta agagaatggg tatctctatc aaactatctt tcaacttctc
    18901 tattaacaat agcgtttctt ctctattata tatttcaatt tcatatgtaa cattttgagc
    18961 tggcaatttt acaaaatcgc aaggattttc agatatttct tttagcctat aagcctcttt
    19021 taaaacagaa gatagaaaat tatatctgac ttttgcactt gaggaagtag aattattgaa
    19081 acttttattt ataaagaatt gaagtaaact aggatttata tctataagtt ttatatcttt
    19141 aaaaaaaggt tctatataat tattaaccca agattttcta tttacagttg tgtaaggaga
    19201 ccaatttttt tcatttgcaa ttatgtactt ataacatctc tctacaagtg ttattttgtt
    19261 tggagttata aacttattat tgtttatagt agactttatt tcaattaaat gtttttcagc
    19321 atcctttttc ttttcatagc ttccataact tttttgcttt attttgccag ttgtttcttc
    19381 tatgtactcc acatatacat gaaatttttc ccctcttttt cttataaaag ctgatttgat
    19441 attcatatat gtacactcct ttataagtat tattcatgtt taaaatatat tgaataacta
    19501 tggttttatt atatcaaatc taagttataa attctattcg atgatagagt agatgtatac
    19561 agaaatatat agacaaatct ttaaaaaagt aatatatagg taaaatatgt aagaattata
    19621 tgttataata tttttagcaa ggaaaataaa attgaaaagc gaagggtgat tttctcatat
    19681 aaacgccaaa ttccaatata ggaaggaggt gaatttatat gttaattaac tttttattga
    19741 gtatagtagc tggtattgta tcagctttca tatatgacaa aataaaaaat cacccaaacg
    19801 ccaataaggg tgatttaaag aaataacttt taaattctaa tcaattagag aaattcacct
    19861 tttaaaaagt aaattttatc tccttgctat tattatatca caaattggta tagatattca
    19921 aaaataatat tttattaaag tataattgtt tgaatatatt taagctaatt tattatttaa
    19981 ttcttgtatt tttaatgttt gtctatattc ttgtgcttct gccaattttg tatattccac
    20041 agtcttatcg aaatttttct caacttctgt tcttatttca tcaagcgtaa ctttgaagaa
    20101 ttcctttcta ttatttacct tgttaactct cttattatca aaagctttat gtaaagctga
    20161 ttctaattta ggtgcatctt cactaaatat agtcgcatgt atatcatatt taaaaggaac
    20221 tgatgcacta cttaattcat ttattctctc agttgggtct aatcttctag ttactcctat
    20281 tttatatata ttttctccaa aactaccaat attggagatt atgtatacat gtcctgctct
    20341 tgtattttgt tctctattta atacatcatc taaatctttt gatagcaaag ctagtttttc
    20401 ttctatagaa ttaatttttt gttcccattc taatttatca ttttcatctt ctgggatagt
    20461 tgattttagt ctaagtaatt cattcttgaa ttgtaattct tcttttttta gttttctttt
    20521 ctgattttca atttcttttt gaacaagagc ttcttctttc atttgttctc tcaaagctct
    20581 ttgttcttct ttttcctctt ctttcatttg aaggtattca taatataaat aaagctcatc
    20641 tattttcaaa ttaagatagt tttcttttat agaaacctta tttgaagtat ttaatttatt
    20701 tgtatcagta aatgcttttc gtattctttt ttctatagaa tcaatatttt taaaactaac
    20761 ttttgacata gcaatgtcgc actcactatt gaagagttta agagctgttt taatattttg
    20821 attagtcata gttctacctt tttgaacact tccatcaaca gtccaagaat cagaatattt
    20881 tacacctgtt ttatttctta ttaatagttt ttgcatttct cttatttctt ttaatttttc
    20941 catatattca tcagagtttt caaaatcata ttttggatta tataatccaa atgattgaag
    21001 cagaacctca tcttcagtct gtattaattc ttctgataat ctttttagct cttctttttt
    21061 attttttaaa gttgtgttta tatctctaag ctcttccctt tcatttttta attcttctaa
    21121 atgagaattt aaagtattaa tattatcttt aagtatattt ttatctttta taagattatc
    21181 aagtctttct ttttctattt ttagttcttc gataagttta aatttatctt ggttttctga
    21241 atcaaattta tcaatttcaa ctttcttttt gtctaataga ttggataatt cttttaactt
    21301 attttcattt gattcacata aatcttcaaa gtgtttttct ttcattttaa tttcatcatt
    21361 taataaaata tatttattat tgattttttc gacatcagat aatagtatac taatgctatc
    21421 tttatgtttt ttcattatct tatttctttt aaataagaat attattgcaa ctataaatga
    21481 tatagagtat gttgaaaacg tagaaagaat tagtatggat atagtccata aagaataata
    21541 ccagggtatc ttgaaattaa gatttttgat tttttcatcg ttgatgagta aatcactttt
    21601 taactgttca ttttcattgt tcatatcaaa actccttttt tattttttgt tctactttat
    21661 gataataata atttaagtat ataaaatttt atgtgcaatt tccatatatt tgcaaaaaaa
    21721 ggtaaaaata aaacaaaatg gtagttattt aaaagaattt agttggatta taaaggtatg
    21781 ttaacagaaa ttagaaaaat aatataacat tcttataaaa gtatgttatc agtattttga
    21841 gcataaggaa aagccactta aaattagttt taatcatttt aagtagctct tttattattt
    21901 tagatactcc tttgaatatt taatccaagc atcaatacca tcgattaagt gaattaattc
    21961 gccataaata tgttctgaac caaattcatt tttcatgtac attatccatt tttttctacc
    22021 ttgcaattta actttaccaa tgaagttgtc tgaagtatca aatacattaa atgctccatc
    22081 tgacattcta tcaaagacaa aattattttt taattcattt tcgtacattt taattttaag
    22141 tatattaaaa aagtagattt cattttcatt aattttgaag ttgtaatttt tattattaac
    22201 ttcgaatggt attgatttgt tatttttaac aatatccata taatgtaaat ttttattatt
    22261 aatactatca tttgcgttat atgttatagt agagtgattg tatattacat catttatact
    22321 atttatagca ttattaattt cgatattatc aattttttct cctgccaaac gttttactaa
    22381 tatagaagtt ttggagttat ttcctaacat atcacaaagt atataggatg atttaattat
    22441 gttatctaaa agtgtgtctt ttaaatttat atctaattcc tttatggctt tgtaagactc
    22501 aattttcata ggtgtaattt tatgattttc ccagtttata ttaattcctc tttggatatt
    22561 tttagaggat tcatatttac aaattaagtc atatgcttcc tcatatttat caatataaat
    22621 taattctaaa cactggtctt ctaggtcagt atttttagta acagatttac gcacatcttt
    22681 caacagttct tttcctttat ctgttaaagc ataatatttg ttggagctat taaaaaaatt
    22741 agataaatct ttgcatgaga tttctttttc tatcctttct attaattcag cctttttacc
    22801 agttgattta atattattag attttaatat ttctttcaat tccgaaactg ttaacttagt
    22861 aaggtcttca atgtaatttg aaatcttaag ataatccatg ttgaaaaact tagaaattaa
    22921 atatgaatag ttaatattat attcatatgt ccaataggtt gctatataag gctcgtatat
    22981 attttctcca attatgtatt ttaggaaaga tttttctatt gatgtaagtg gtttagtatc
    23041 tgtttctaat atattgtttt gtgggttaaa cttattaata ctattagtat gtatatcata
    23101 taaagcatct gatttttctc ttgcttgttt ttctaattca tataaatttg ttgtatcatt
    23161 acaacttaca ttcagcactt tattaatttc attttttact tctatgttat gaggtgaatt
    23221 agaattacat tctagtgaaa gtgtagtact atcattatag ctttgttttg attcgttcga
    23281 tttttcattg caatcaacat tatttttgtt tttatttttt ctaaatagat tcaaaatgct
    23341 cataaaaaat tactcctttt gtatttaatt aatttctagt gtattctact ttgtgacaat
    23401 aacaatttaa gtatataaaa ctttctgtat aaatgctatg tatattagga aaaaacatag
    23461 aattaaatag ttttgaaaag aatactatat ttatatggta taaaaagtaa aacgaagatt
    23521 ttaaaattaa ataataaaaa atattatata aacaagaaaa tacaaaaaat tataattaaa
    23581 aagacgatta ataatacaca ggaatggaaa aaataaccaa gactctacaa aattcgattt
    23641 ttttgttaca attccccttt ttttattgca tatatttaac aaaagtatta ttatgtaagt
    23701 aagataatta tccaagaaaa ttttgaataa tctaaaaata tttttagaaa tgaaaataca
    23761 tagttatatt ttattgaaat ttgttgggaa ttttaactaa aaataagaac ataagttctg
    23821 atagtagggg gaaggcgtgt ggaaaaatca aaaaaactac taattgacac aggggataca
    23881 ttgaacaaat taagagaaaa taatagtgag aagttagaaa agtacataga attattaaaa
    23941 gaatattata aacaagaaaa taaaaaagaa agagaatagt tttcttctct ttcttatttt
    24001 ttctcattaa gttcttcttc aagaaatact tttgctattt tgaacatttt ttcttgacta
    24061 gaaggactta attcgctaat tagttttaaa gcttctttta tatcatcaga gaaatttaga
    24121 ttttcaatta gctctaactc actaagattg tctatatttt ttacattgac ctttccaagc
    24181 aaataatctg tagatacatt aaaaaaatct gataatttta aaattatgtc atgtggagga
    24241 aatctttctt ccttctcata aaaagaaatc attttaggag taagacctaa aaaagttgca
    24301 agttcttttt gagtaatatc tttttctttt cttaattcct ttattctata tccaatcatg
    24361 ttatttagcc acctttttaa tttatattga actattagtt cttaattatc aattatagct
    24421 catttttttt aaaaaagcta ttgacaatgt actaatagta ctgtactatt agtacataag
    24481 ataaagtaaa agaaggtgaa aaaatgaata aactaaaaga ttttagaata caaaaaggac
    24541 taagtagaaa tcagttagga gctgaattag gtcttacaag taggtatata gctttcttag
    24601 agaatggcga aagagtaccg tctctaaata cagctgtaaa aatagcagct tatttcaata
    24661 cttcaataga gtatattttt ttgaaaaaca actgtactaa aagtacattt aaagaaaaag
    24721 gagctgaaaa atatgaataa cttacaagta atagaaagaa ataacgaaag agttttaact
    24781 acacagcaac tagcagatgt atatgaaaca gatgcaagaa atataagcaa caattttaac
    24841 aataacaaag atagatttat tgaaggtaaa cattattttt tattgcaagg tgatgattta
    24901 aaaaatttta aaggtattca tacagaatat gaaaacctaa aatttacttc aaaaatgtat
    24961 ctttggactg aaagaggagc aaatagacac tgcaaaatat tggatactga caaagcttgg
    25021 gagcagtttg ataacttaga agaaacatat ttcaaagtta agcaacataa gccaacttgt
    25081 atagaagatg tattaataga aagtttaaaa gaaatgaaag atttaagact tcaagttaat
    25141 caagcaaata gcattgcttt agaagcaaag acagaggttg aaacaataaa agatgtagtt
    25201 tcattagact caaatagttg gagaacaaat acacatcaac taattgcaag aatagcaaaa
    25261 aaacaaggtg gttttgaaca tataaatatg cttagaacag aaagttatga attattaaat
    25321 aagcgatttg gagttgattt gcatagaaga ttaattaata aaagaagaaa aatggcagaa
    25381 gaaggtgtat ctgaatctaa aagagagaaa gttaacaatc tagatgtaat acaagatgat
    25441 aagaagctaa tagaggggta tgtggctatt gtaaaagata tggctttaaa atatggaata
    25501 tcaagtgatt taagcaaaaa ttaggttaaa tcatgacagt accttgaaaa ctaaatacag
    25561 aatatttaat taatatgaga aggaggtttg aaaatgagtg tagcattaca gtttatagat
    25621 acagaagatt tagtaaaaga attaatggga cgagaggata cagtagattt cataaaaatg
    25681 tttttagata gagaaggaat taaaagaatg gagttaatga ctatagaaga attttgtgag
    25741 tacttgaaaa tatctgatgt aacagctaga aacatggcaa gagaagccat gataacaaaa
    25801 gattttattg ctttaaaaat aggaagaaag tacatgattg atagaatatc atttgaagaa
    25861 tttattatga aaaatgcaat gaaagataga gatgtaatga aaaaaagaaa gggggtgatt
    25921 tagttgactc taagatggtt tctaagattt tgtataagac acaagaaagt acctacacca
    25981 aagttttaca tagagtgtgt aacttatatg gaagaatgta aacaaagagg acttggactt
    26041 taaggaggat tgaatatgga agtaactaaa agatttttag aatataaaat acaggctcta
    26101 agcgagagga tagaatttaa aaaaacaatt ggatataagt gtatagcaga tgaaaaagaa
    26161 ctaggagcat atgaggatat tttgcttatg ttaaattcag aaattgaatc aataggagga
    26221 gtggaaaatg aagagtagac aagaattaat caaagatata gaaaaataca gaaaagcaca
    26281 atacttaata tatttagata tagtacaaag agcatgggca gatagaagcc ttacagcaga
    26341 tgaacaagac agaattaaac aagaagcata tgcagagtac aaaaggatag aaagagatac
    26401 agaagaagca gaagaactgc taatgagaga agaatttgaa acagatagac ccttagcagt
    26461 tcaaataatg tagaaaagag ccactgcaat ggctctaatc aaaaacaaaa aataaattta
    26521 ataattatat attatagcat aaatggaggg aaattatgag tactttatat gaattagcta
    26581 cagatttatt agaaatagaa gaaggtttaa cagaaacaac aagaaatgaa gctgaaaaac
    26641 tagaggaaat aaaagaaata ataaaacaag agatacaaaa taaaaacact aggatagttt
    26701 cagtaatatt aaacattgac agtgatataa actctataga ttcagagatt aaaagattgc
    26761 aagagttaaa aagggtcaaa aagaatactc ttgatagatt aaaaagcaat ataaaagact
    26821 gtatggaatt acttgatact aaaaaagtag aaacattttt aggaaatata agtataagaa
    26881 agtcagcagg tagcttagtc atagaagatg aagaaaagat acctgctata tataaaacag
    26941 tagagcaagt tgtaaaagta gataagaata ccattaaaga ctttattaaa aaaggtcatg
    27001 aggttgaagg ttgtaggatt gaatattcaa ctacattaac aactccaaaa gttaaaaaag
    27061 agtaggtgaa aactatggaa aataataata tttatataaa acttgtgaat atacagagta
    27121 ctttaaaagc tcctaaaagt caatttaata gctttggtaa atacaactat aggagttgtg
    27181 aggatatact agaaggttta aaacctattc taaaggaaga aaaagcatta gttatattgg
    27241 atgataatat tgttcagata ggaaatagat tttatgtaga agctacagca actttaatag
    27301 atgcagaaac aggagagaaa gtatctacaa aggcattagc tagagaagat gaaactaaaa
    27361 aaggtatgga tttagcacaa gtaactggaa gtgtatcaag ttatgcaaga aagtacgctt
    27421 taaatggatt attttgtatt gatgatacaa aagatagtga tgcaacaaat aaacatggaa
    27481 atgagcagaa aaaaaaagaa gttaatgaga gtgaattaaa tacactatat tcgctaggag
    27541 aatctataga aaaagataaa aatagagttg atagtgaagt atataagaag tttggaaagt
    27601 tagcagtaga tttgactaag caagagtatg agaaagtttt aaatggatat aagagcattt
    27661 tagagaagca aaaacaagag taggtgatag tattggggat tataagagta agcaaagaca
    27721 aggataatcc atatgtagtt ttaaataaaa cttgtttgga agatgtgaaa ttaagctggc
    27781 aagcaaaagg tttacattca tatctgatta gtaagcccga ccactggaaa atctatgtta
    27841 atgacctttg caagagaagt aaaaatggaa gagatgctac agcaaatatt ttaaaagaac
    27901 ttatagaaaa tggatatata acaagaaaac cttgtagaga ttctaatact aataaaatgc
    27961 ttggagggta tgattatgaa gtatatgaga taccacttga aaatcctcag aagctaaaat
    28021 cccgaaaaac tgatttcccg gaaaccggat ttcccggaaa ccgggtttct cggaaaccgg
    28081 aaaacacgga agtagtaagt aatgacttta aagtaaataa tgatattact actattgtta
    28141 ttaatgaaca atccaataaa gacaaaacca cctacataaa aaaatacttt gaaaaatata
    28201 taggtgtgat tactcctaat aactttatag agttaatgag ttacttagat gatggaatgg
    28261 aagctgatgt aattataaga gctattgatg aagcaatagc aaatggagtt aagaattata
    28321 agtatgtaaa gacaatatta aataattgga tagaagcagg tgtaaaaact aatttagaac
    28381 ttacagagta tcaaaatgag tttgagagga agaaaaagaa taaacaggat aagaagcagt
    28441 ctaatagtaa agctgtgaat actcctaatg tgagtaaaaa taagtttcat aacttcaatg
    28501 aaacatttac tcaatattca cctgacgaac tagatgacat aattaagaaa agccaaaagg
    28561 ttaaatttaa ataaaattaa acttctagga agtaaatatc aatatattgc ttcctagaaa
    28621 ggggaggtat aaatggcgag aatatatgca caaagaagtg gttctttaaa tgaacaagat
    28681 agattggaat tattaagatt acttgggaaa gctggatata cagtaaagat tgctagagag
    28741 aagcaaaata gcaagacaac ttatacttac tttgttgagt atacagaaga gcaagaagaa
    28801 aaatagaagg gggctagtta aatgaataca ataactttag ttggaagatt agttgcagat
    28861 gcagaattga agtaccttcc aaattcgggt actccaaaaa taaccttttc aatggcagta
    28921 gatagaaggt ttaaagataa aaatggaaat aaaataactg attttattca atgtgagcaa
    28981 ttaggaaaac atgtagagaa tttagtgcaa tatcttgtta aaggtaaacc tatatatgct
    29041 gttggagagt taaatatata taattacaaa gatgaaaatg gttgctggaa atctattact
    29101 aaagttaacg tgaatgcttt agaattactt tctagtaaaa gtgatagtaa taatcataaa
    29161 gagcaacagg aatatatacc accaggactg gacccacaag gttttcaagc aatagatgat
    29221 gacgatatac ctttttaatt aagttaaata atctagggag taattatgca atattacttc
    29281 ctaggagtta aaatattgga gggatggaaa atggaagaag agtatagaaa atttttttta
    29341 gagcgtaatg gtgagaaaat tgagatggta caaaattgcg aaggagcaat aaaaataagc
    29401 ttaaataata taaaaacaga taattcgcat agaccttgga atttaaaggc agaaggaaca
    29461 gctacagtga cgctatatct aggtaaactg ctttttgaag aaatgctgtg gcttaaaata
    29521 ttgtatttat tagaagttat agcagaaaaa atgatgatgg gggtattagg aatgataata
    29581 attagaagtc aaaataaatt agatttaatg agagttaata gagttgaaat agatagtaga
    29641 tatgtatatg cagtgtttga aggtgaatca aatgttagag aaataggtag gtataaatca
    29701 gaagaaagag ctattgaagt attaaacaga atacaggagg ctattattgc aggaactaag
    29761 tttgacatta taaataaaga cggggttaga tgcaacaaag aaaaagtgtt tgaaatgcca
    29821 gttgaataag gagggggctg aaatgttaaa ggttgaaaga tattttagtg gttctgttgc
    29881 agataatata tttgaagatg atttgactct tagaaattat ttagcactat attgctgtat
    29941 ctatggagta aacaaaaatg gagaacttgt atttccaaca cgtggaaaaa tgctaataga
    30001 gtttaatgtt gatagggata gaaaaaagag aaagaaagct tcaaaagtaa agttaattaa
    30061 tgtcaaaact ggtgaagaaa aaatatttga ttctatagat agtgcagcat gttttttaag
    30121 actacagagt caggcagttt accaaacaat taaaaagaaa actaaaacaa gaagtggctg
    30181 gaaagctgaa tatattgagg aggaataatg gaagtttcaa ggactgaata cacaattaaa
    30241 agagcaaaag agttatatga taatggagag gatatattta ttgctataga taaggctaga
    30301 gaagaatatg aggagatggt taaaagtgaa tatcttagct agtgtgatat tagtaatagg
    30361 aagttttata gctggtagag tttatgagta tagattgaat ttaaatgaaa atgatgaagc
    30421 tgattcaaaa gtacttttag atgtttttaa tgaaattagt gtgttaagag aagaaaataa
    30481 aagtcttaaa gaaaagttac aagagaaaga gttactattc atcaatagat taatagattt
    30541 tttacatgat aaaaagatat gcgaatgttg tatttatgac tgtaagattg atgatattga
    30601 atatgattgt gaagatggta ttaaaaagtg gcttgatagc gaagaactta tatttgaata
    30661 gaagaaatat ctaattaaaa cagtttagag agttgcaaaa tgttttttaa taaaattatc
    30721 attgagatgt tttgtaactc tcaaaaatga aaa
//
LOCUS       DAMJZE010000026        30719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJZE010000026 DAMJZE010000000
VERSION     DAMJZE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.026"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            123..3671
                     /locus_tag="PC541_001124"
     CDS             123..3671
                     /locus_tag="PC541_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2838595.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3856..5790
                     /locus_tag="PC541_001125"
     CDS             3856..5790
                     /locus_tag="PC541_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2838596.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5896..6891
                     /locus_tag="PC541_001126"
     CDS             5896..6891
                     /locus_tag="PC541_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2838597.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6863..7648
                     /locus_tag="PC541_001127"
     CDS             6863..7648
                     /locus_tag="PC541_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2838598.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7636..8334
                     /locus_tag="PC541_001128"
     CDS             7636..8334
                     /locus_tag="PC541_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838599.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8457..8930
                     /gene="ybaK"
                     /locus_tag="PC541_001129"
     CDS             8457..8930
                     /gene="ybaK"
                     /locus_tag="PC541_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2838600.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9127..9966
                     /locus_tag="PC541_001130"
     CDS             9127..9966
                     /locus_tag="PC541_001130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2838601.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            9979..10527
                     /locus_tag="PC541_001131"
     CDS             9979..10527
                     /locus_tag="PC541_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2838602.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10593..11774
                     /locus_tag="PC541_001132"
     CDS             10593..11774
                     /locus_tag="PC541_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2838603.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12069..12455
                     /locus_tag="PC541_001133"
     CDS             12069..12455
                     /locus_tag="PC541_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2838604.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12605..12868
                     /locus_tag="PC541_001134"
     CDS             12605..12868
                     /locus_tag="PC541_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2838605.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(12997..13839)
                     /locus_tag="PC541_001135"
     CDS             complement(12997..13839)
                     /locus_tag="PC541_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2838606.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14134..14868
                     /locus_tag="PC541_001136"
     CDS             14134..14868
                     /locus_tag="PC541_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2838607.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14878..16011
                     /gene="nagA"
                     /locus_tag="PC541_001137"
     CDS             14878..16011
                     /gene="nagA"
                     /locus_tag="PC541_001137"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2838608.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16110..16859
                     /gene="nagB"
                     /locus_tag="PC541_001138"
     CDS             16110..16859
                     /gene="nagB"
                     /locus_tag="PC541_001138"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2838609.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17034..17306
                     /locus_tag="PC541_001139"
     CDS             17034..17306
                     /locus_tag="PC541_001139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838610.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            17966..18670
                     /locus_tag="PC541_001140"
     CDS             17966..18670
                     /locus_tag="PC541_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838611.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18672..20159
                     /locus_tag="PC541_001141"
     CDS             18672..20159
                     /locus_tag="PC541_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838612.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20364..21977
                     /locus_tag="PC541_001142"
     CDS             20364..21977
                     /locus_tag="PC541_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2838613.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22340..23170)
                     /locus_tag="PC541_001143"
     CDS             complement(22340..23170)
                     /locus_tag="PC541_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2838614.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23243..24970
                     /locus_tag="PC541_001144"
     CDS             23243..24970
                     /locus_tag="PC541_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838615.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25007..26869
                     /locus_tag="PC541_001145"
     CDS             25007..26869
                     /locus_tag="PC541_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838616.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27202..27891
                     /locus_tag="PC541_001146"
     CDS             27202..27891
                     /locus_tag="PC541_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2838617.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            27996..28682
                     /locus_tag="PC541_001147"
     CDS             27996..28682
                     /locus_tag="PC541_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2838618.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28699..30606
                     /locus_tag="PC541_001148"
     CDS             28699..30606
                     /locus_tag="PC541_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2838619.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT        12133 a         3216 c         5384 g         9986 t
ORIGIN      
        1 ttctaataca ggaagaaggt aaatgtatat tgaaaaaatc atagaaatag aagtaaacaa
       61 tttaaataaa tacaaaggtt tgcaaaagaa gtaatataat tatatacaat gaggatgtga
      121 gtatgatgaa cagactaaag ccaagaattt taggtattag tatcttgttt attattataa
      181 ttattttaac atgtggagct gttttcaagt ttaaatttaa attagataat aaattaaaag
      241 aaaccacaca tacatatttg tctgagttta cagaacacaa tataattaat ctcaagacaa
      301 gacttagtgg tcaatttgac atgcttgaat ctattgctaa cttcatagga aacttgaata
      361 atataaatga tgaaatggtg attgatttaa tggattctga tgtaaagcga agttctttaa
      421 ttagaatgac tatctctaca ttagatgggg aatgttattc taatgatgga attacccata
      481 atgttcaaga tagagattat tttaaaaaag ctaaaaatgg agaaaagaat atatcagagc
      541 ctttaaaatc tattgttaat aataaagaaa taatagtttt atctacccca gtttataaaa
      601 gtgggaaaat agttggtgtt ttaagtggaa tttatgattc aaaaaagtta aatgagttat
      661 taggattatc tgcatttgat gaaaaagcag atgtttttgt tgcaaagaaa aatggagata
      721 tagttgctca aaaaacaaat aaaaatagaa agacgctatc atgtatattt cagctttttg
      781 acaaagataa ttgtgatgaa gatgattgtc taaaaactat aaaagaaaat atgaaaaatg
      841 ataagtctgg atattttaat tataacttag ataatgaaaa atttatgtta aactatcagc
      901 cacttggagt taatgattgg tatgtttttt ctgttgtgcc aaataaaata gtatctattc
      961 agtcagagaa tatatcttat gatgtatata tttttgcatt agaaatacta ctagttatat
     1021 tgatttttat aatatatata gcatgtatta taaataataa caccaaaatt attataaatg
     1081 aaaaagaaaa atttaaagta ctcactgata atatattagg aggggttaaa acctgtttaa
     1141 aagatgaata tatgactatg acatatgtaa gtcaggggtt tttagagctt acaggttact
     1201 cttgtgaaga tttaaataaa atatttaaaa atagatttaa agacatgatt tataaagatg
     1261 atttagacaa gtttaattgt attattctaa atcaattaga aaaacaagat aaaattgaag
     1321 ttgaatatag aattgtaaga aaagatggaa gtataatttg gattttggag agaagtaagt
     1381 ctataaatga taataaagga agtgagtgta ttcagggagt tttaactgat ataacagaac
     1441 ttaagaaaat acaagaagaa ttagattcaa aaagatttga aatacaggct atcaatgata
     1501 gtattgttgg tggaatagtg ataactgaga taaatgatga ttttgatagt atatatttaa
     1561 atgaaggtta tttaaatatg attggatata cacgcaaaca actggaagat gagctaaata
     1621 ataaattaat caaacttatt tttaaagatg atagaacaga attaattaat agtataaaca
     1681 aacaactcaa attaagtgat acatttgcat ctgaacaaag agttgaaaaa agggatggca
     1741 ctattatatg gattttatta aaaggaagaa agatgattta tgataatgga aaatctgtac
     1801 tttgttgtgt tattgtagat attacttctg ctaaattatt agagcaagaa cttaaaaaga
     1861 gtgaagaacg tcatgaaatc attctaaagc agacgcaaaa tattataata gaatgggata
     1921 tagaaaaaga taaaatgaat atatcaccta attggtataa taagtttaaa tatgacccta
     1981 taactgaaaa cgcaacaaaa aatataatta aatcagtaga tatatatcat gatgatatgg
     2041 ataagtttat ttcattaatt aaacttactt cacaaggtag tgagtatgaa gagtgtgaaa
     2101 ttagacttag aactagagat actatttacg cttggtttag tataaaggca acaactttat
     2161 ttgataataa tggtaaacct tataaagtag taggaagttt gactgatatt gatgaatcaa
     2221 aacgagaagc tgaaaagttg aaagaaaggg cagaaaaaga ccctcttaca gatctgtata
     2281 ataaaaaagc atcacaatca cttatagagg agtacataac attggataaa aaaagaaaag
     2341 gtgctttaat gattattgat attgatgact ttaaaggaat aaatgataat ttaggacatt
     2401 tatatgggga tgctgtatta agtgaaattg caaatgacct aataaatcta tttagagcaa
     2461 ccgatatagt aggcagaatt ggtggagatg agtttattgt atttataaaa gatatttctg
     2521 aaacaagaga tattataaat aaagcagaag aattggtaaa aacatttgaa aggtcttttt
     2581 taggtaaagg acgaaattat aaaatatcat taagtgtagg aattgctaag tttccacaag
     2641 atggtatgga ctatatgaca ctatttagaa atgcggatat agctctttac tcagccaaag
     2701 ggaagggcaa gaattgttat acattgtatg ataaaaaatt tgataaagtc caatatataa
     2761 ctaatacaag aaatgcagga gatagtgata atgtagagat gtcttttaaa gaacatatca
     2821 caaattatat atttgatata ctatatgaat caagtgatat agaagtggca gtaaatttaa
     2881 ttctaagtat tgtaggtaaa cactttaatg tgagtcgcat gtatatattt gaaaattcag
     2941 atgataatcg agtatgtaaa aatacatttg aatggtgtaa cgaaggtgta acctcggaaa
     3001 ttaataatct taaaaatatt tgttacaagg atttaggcaa ctatctttta aattttaatg
     3061 cagatgggtt attttattgt aatgatattg aaaatttagg tagtgatgtg aaaaaagtac
     3121 ttaagccaca aggcatacgt tcaatattac aatgtaaaat tttagagggt aatgaattta
     3181 gagggttcat aggatttgat gaatgttcat ataatcgtat gtggactaat gatgaagtga
     3241 acactttgac atttatatct aaaacgctca gtatgttttt attaaaaatg cgtattcaga
     3301 gtaagctaga agagtcttta acaattcaaa aatctgtgat ggataatatg gatatatggg
     3361 catatgttgt tgacaggaat acctatgaat tactatttat caataaaaaa atgcttgagt
     3421 cagcaccaaa cactcatgta ggtgatttat gttataaagc gatatggaat aatcaagata
     3481 aaccttgtaa aaaatgccct atgatatatt taaaagatgg tgatgatact tataaatcat
     3541 tcatgcataa taaaaagttt gaggtacagg caaatataac tacaactact ttgaaatggt
     3601 ttgatggtaa agatgcatgt ttaatatttg gaagtgaaat aactagtatt gaagctgaag
     3661 aaaatactta aaatactgtg ttatatatat acttaaaatc gcaatttgaa tttattagac
     3721 ttaaaatgtc tatataaatc atttaagttg tggttttaag tattttttat gatataatta
     3781 agcgttaaga acaaaattaa aagatacttt tgtataaaaa aatatttgtt aaatatatat
     3841 tgagcaggtg ataatatgga aaataagttt ttaccaataa gcaaacaaga tatgatagat
     3901 agaggatggg aagaattaga ttttgttcta gttacaggag atgcttatgt ggaccatcat
     3961 agttttggaa cagcaataat atctagagtt ttagaaagtg ctgggtataa agttggaata
     4021 atagctcaac cagactggag aactacagat gattttatga agctaggaaa acctagactt
     4081 gcatttctag ttaatgcagg aaatatggat tcaatggtca atcattattc tgtaagtaaa
     4141 aagcatagag aaaaggatat gtattctcca ggtgggaaaa tgggttatag accagataga
     4201 gctactatag tatattgcaa taaaataaga gaagcataca gtgatgttgc aatagtaata
     4261 ggtggaattg aagcgagctt aagaagattt gcacactatg attattggag tgataaggtt
     4321 agaaagtcta tgttaataga tagtggagca gatttattag tttatggtat gagtgaaaag
     4381 cagattgtag aagtagccaa tgcacttaat gatggatatg acccaaaata cattagacat
     4441 attgatggga catgctatat ttcagataca ttggaagaaa tatatgataa atatatatta
     4501 attccatcat ataaagaaat ttgtgaagat aaaatgaagt atgtagaagc ttttaaaatt
     4561 caatatgatg agcaagaccc atttagaggt aatataattg ttcaacctca tggaagtaag
     4621 tatctggttc aaaataagcc agaaaaacca cttagtagag aagaattaga tgaagtatat
     4681 ggattaccat atcagaagac ataccatcct gtatatgaaa aatttggagg aataccagct
     4741 atagaagaag taaaatttag tatagtaagt tcaagaggct gttttggtag ttgttcattt
     4801 tgtgcaataa catttcatca aggtagagca gtgcaaagta gaagtgaaaa atcaattatt
     4861 gatgaagcaa taggaataac aaatttagat gattttaaag ggtatataca tgatgttgga
     4921 ggtccaacag ctaattttag aagaccagca tgtaaaaaac aaattactaa aggtgcttgt
     4981 aaaaatagac aatgcttatc tccatcacca tgtaaaaatt tagatgcaga ccacagtgaa
     5041 tacttacatt tacttagagc tgtaagaaaa ttacctaaaa taaaaaaggt gtttgtgcgt
     5101 tctggaatta gatacgatta tgtaatggca gataagaaca ataaatttct tagagagttg
     5161 atagagcatc atgtaagtgg tcagttaaag gtagccccag aacatatatc agaagaagtt
     5221 ttaaagtata tgcaaaaacc agcaggaaaa acttatgata aatttagaca gaagtttttt
     5281 gctataaatg aagaattagg taaaaagcaa tatttgatac cctatttaat gtctagtcac
     5341 cctggttcta ccttaaatag cgctatagag ttagcagagt atttaagaga tactcactac
     5401 caaccagagc aggttcagga tttttatcca actccaggga ctctatctac aaccatgttt
     5461 tatacaggta ttgacccgct tacaatgaag ccagtttatg ttcctaaatc aaaacgtgac
     5521 aaggcaatgc aaagggcttt attacaatat agagcaccta gaaattatga tttggtatac
     5581 tctgcgttag ttgaagcagg aagagaagac ttaattggtt ttggacatag atgtcttata
     5641 aagccaaaaa atgaaaagcc ttattttaat agaaataatt ctaaaaaaaa tgtatctaaa
     5701 ggaacaaata aaaacaaaaa aactaataca aataacagaa gtaataaaaa tcaacaaaaa
     5761 agttcaacta aaaaaaagaa aaaacattaa agtataaata aaaatactta aaaaagaagg
     5821 attattcgat aaattaacag aaattagtat aaataaatat aattatttta atgacgttac
     5881 ctttgggagg aagagatgaa aaagtatatt tctataatat tattagtggt gttgactatg
     5941 gttgttttgg taggatgttc accaggaaaa gataacccta aaaataagga gctatcagtt
     6001 gtcaaacaag taaaagtagc tgttccagat ggactaccag cagtggcaat agctaaactt
     6061 gcaaatgaaa atccagagat taaagaagga tatgaaacag tgtatagcat tgaaaagaca
     6121 cctgaagcga tttccactag agtgatgaaa aaagatgcag atatagctat agttccttca
     6181 aacatggctg caatagcgta taataaaact tcatcatata atattgttgg aactgtagga
     6241 atgggttcac tttatttggt gtcaacagaa aatataaaag attactctga tttagttggt
     6301 aaagaagttg gatgtactgg aaaaggactt actccagaca taactataaa aagtcttttg
     6361 ttacaaaagg atataaatta ttcagatata aaatttaatt atgtaaattc agcaagtgaa
     6421 ctggtaccat tattggctac tggtaaagtt aaaacaggta ttgttccaga acctgcactt
     6481 agtgctttga tgtctaaaaa tccagacata aaaattataa aaagtttaaa tgactcatgg
     6541 aaggaagtaa gtggttcaaa agatggttat cctcaatcta ctttaattgt aaagtctgaa
     6601 tttttaagag ataataaaga ttttgttgat tcttttgtag gtcaattatc aaatagtata
     6661 gattgggcaa acaaaaatcc tgaagaatta ggtgcatatt ctgaaaaaat aaaaatatct
     6721 actgaatcaa agataatagg aaaatctctt gaaagagcta atttgaaata tattccagtt
     6781 aaggacatga ttaaagatta taaaaattac tatgagaaat tagctaattt tgatgataag
     6841 acattaggag gcaaagtacc agatgaagca atatactttg ttgagaaata aaaaaaataa
     6901 agaacaattt atagataaaa tggaggtaac actttcttgt gttatcctct tatttttatg
     6961 gcaaattatt gctttaaaaa ttgataatga tattttttta ccaacagtag gtcaagtgtt
     7021 taatagtata aaagagatta tgctaaattc tagtttctat atagatatac tttactctat
     7081 ggggagatgt ttttttagtt ttttactggc tatgattttt gctattatac tttcaatagt
     7141 gtcttattta aatagatttt ttagaaactt attaaagcca atcaatgctt tgacacgttc
     7201 aataccaact atgatattgg tagtacttgc acttatttgg tttgaaaagg atagtacccc
     7261 ttttatagtt ggatttacta tagtttttcc tatattgtat gataacgttt tgggcgctat
     7321 attgaatata gacaagaact tattagaaat ggctaatgtg tataaaataa ggtttataga
     7381 taaggtgtta aaaatttatt taccagccat aaaatttcaa attatatcta tactagtatc
     7441 aacattttct ttaagtttga aagttgctat agcaggagag gtgtatagtc aacctacata
     7501 tggtattgga actatgatac agactgaaaa aattaacttt aacacatctg gaatatttgc
     7561 atggataatt attgttcttt taatatctgc tattttacaa atagctcaaa agtttttagc
     7621 aaggagagct tttttatgga aaagataaag ttgagtattg aaaatataaa taaaagatat
     7681 gatagtcgta ttatatttag agattttaat attgattttt atgtaaatga ggtaaattgt
     7741 atccttggaa aatctggttg tggaaaaact acactcttga acataataag tgggattatt
     7801 aaaaatgaca ccaacaatct aaatatcaaa gaaaatttaa atagagtagg aaataagctt
     7861 gaagctagtt acatatttca agatgataga cttatagact ggttaacagt agaagaaaat
     7921 ataaagatag ttgtaaataa atactataat aaaactcaat taaataaaat ttgtgatgaa
     7981 tacttggaat tggttggaat tagtgattat aaaaaattct atcctcaaat gcttagtgga
     8041 ggtattagac aaagagtaaa tattgctaga gcttttatat atccatcaaa aaacattata
     8101 atggatgaac cttttaaatc tattgatgca aagaatacac agcttataat ggataatttt
     8161 agaaatatat taagaaaaga aaagagaact gtgttatttg taactcataa tattgaggaa
     8221 gcattgtttt tagcagatag gatatttatt ttaggagact ctccaattag aataaaaaaa
     8281 atattaaaaa attctaagga attggaaaaa aatgaagttt tgaagttgat atagagttat
     8341 aataatactg tcgttttaaa atatatttgg ttaaaatcta taagactata ttaaaatagt
     8401 acagaatatt caaaactgtg tataatgttc gtattttata aaaaggaagt gaatgtatgg
     8461 ttaagactaa tgctatgaga atattagatt caaataagat tgattataaa gtaatgtctt
     8521 atgaagtgaa aagtgaacat gtggatggtg ttgaggtggc acatgatata ggaagagatg
     8581 taaatgaagt gtataagaca ttagtaactc aaggagttag taagaatata tatgtatatg
     8641 taataccagt acatgaaaac ttagacttaa aaaaggctgc aaaagtagct aaagagaaaa
     8701 gtgtagagat gattcatgta aaagatataa ataagcttac aggttatatt agaggcggat
     8761 gttctcctat aggtatgaaa aagttttata aaacttttgt aaatgaaagt gcaaagaatt
     8821 tagatacaat tattgtgagt gcaggaaaaa taggatatca aatagaacta tctccatttg
     8881 atttacaaag acttataaaa gtagaatttg tagatgtaat aaaaaaataa gaattacata
     8941 taaaatatag agcttatatt ctcaataata ggtgttgtaa tattaataaa atgaataaga
     9001 attttaaaat aaatattcag ttacttatgt gtaattggta taaaaatatt gagaaatttt
     9061 aaaaacaatg gtaaagtgta agtataatca cgaatatttt ttataaagta atggagggat
     9121 gttcgcttga gaaaaataaa atctgaacaa atcgtcgagc aggtaaaaaa actttgtata
     9181 gaagcgagcc tatatttagg agaagatgtt ttaagttgta taaaagaaaa agcgaaaagc
     9241 gaaaagagtg aagttggaaa aaatatattg aatatattag ttgaaaatgc tgaaatagct
     9301 aaagaaaaaa atataccaat atgccaagat actggtatgg ctgtattttt tgttgaaata
     9361 ggacaagaag ttttaattga aggagacaca cttactgatg ctataaatga gggagtaaga
     9421 caagggtatg aagaaggcta cttaagaaaa tctgttgtaa gtccaattaa cagagtaaat
     9481 acaaaagata atacacctgc tgttattcat tatgatatgg taaaaggtga taaaattaaa
     9541 atagaatttg cagctaaggg atttggaagc gaaaatatga gtaagatgaa aatgttaaag
     9601 ccatcagatg gattggaagg aattaaaaaa ttcataatag atacagtttc agaagctgga
     9661 cctaacccat gccctcctat ggtaattgga gttggtatag gtgggactgt ggacaaatgt
     9721 gctcaaatag ctaaaaaggc actttttaga gaactaggtg aatttaataa ggacgaaaat
     9781 atagcaaaac ttgaaagtga actattaaca gctattaata aacttggtat aggacctcaa
     9841 ggcttaggtg gtactacaac agctttggga ttaaacatag agacttttcc tacacacata
     9901 gcaggtctac ctgttgttgt aaatataaac tgccatgcat caagacataa gaatgtagta
     9961 atttaggagg aaaataaaat gataaaaata actacacctg taaatgaaat tgatatagca
    10021 aaattaaatt gtggagatac aattagtttg agtggtatac tttatacagc aagagatgct
    10081 gctcataaaa gattgataga ttgtataaat aaaggtgaag aattaccatt tgacgtatat
    10141 ggtcaaggaa tatattatgt aggacctact cctacaaagc caggagaagt tataggtgct
    10201 gcaggtccaa cgacgagtta tagaatggat gatttgacta tacctctttt agaaagaggt
    10261 cttagattga tgataggcaa gggaaaaaga agtgatgaag ttatagaagg tatgcaaaaa
    10321 tacggagcag tttatcttgc tgcaatagga ggagctggag cgtatatttc aaattcaata
    10381 aaatcatgtg aaattattgc atatgaagat ttaggagctg aagctataag aaagattgta
    10441 gtagaagacc ttaagcttac agtagcaata gatagctatg gaaataatat atatgagcaa
    10501 ggtcgagcaa tttatgaatg taaataatgt ttagaaataa atatgaatat caaaattaaa
    10561 aatagttgtg tatattttaa ggaggaaaat tgatgtctaa ggattatgca aaattggcat
    10621 tagaaatgca tgaaatcaac aaagggaaag taagtgtaga aagcaaggta gaaattaaaa
    10681 ctaaggatga tttatcaaca gcatatacac caggggttgc tgaaccttgt ttaaaaatac
    10741 atgaaaatca agatgatgtt tatagataca cttctaaagg gaatctagta gcagtagtta
    10801 gtgatggaag tgctgtactt ggattaggta acattggtgc tgaagcatct atacctgtta
    10861 tggaaggtaa agctatatta tttaaacaat ttgcagatgt agacgctttt ccaatatgtt
    10921 taaagactaa tgatgtagat gagatagtta aatctgtaga gttgatggag cctgtatttg
    10981 gaggtataaa tttggaagac atatcttctc caagatgttt tgagatagaa gagagattaa
    11041 aaaaatcttt aagtatacct gtttttcatg atgaccaaca tgggacagca ataatagttg
    11101 cagccgccat aataaactca ataaagttaa ttgaaaataa aaaaatagaa gatttagaaa
    11161 tagttataaa cggagcagga gctgctggta tagccatagc aaagatacta cttaatatga
    11221 atgttaaaaa tataatctta tgtgatagaa gtggagcatt ggaagctagt atagaaaatc
    11281 taaactatgt tcaaaaagaa atgttaaaag taacaaatat aagaaatgaa aaaggacctt
    11341 taaaagatat tataaaagga aaagatgtat ttattggtgt ttcaggtcca ggagctgtta
    11401 caaaggaaat ggttgaaagt atgtctgaga agccaataat attggcaatg gcaaatccta
    11461 ctccagagat aatgccagaa gaagcaaaat taggtggagc atttataatg ggaacaggtc
    11521 gttcagactt ccctaatcaa gtcaataatg tactggcttt tcctggaata tttagaggtg
    11581 cacttgatgt taaggctaaa gagataaatg aagaaatgaa gatagctgct gcatatgcaa
    11641 ttgctaatac aataagtgat gaagaaatat gcccagagta tatattacca gatgtattta
    11701 ataagaatgt agtaaaaaat gttgctaagg cagttaaaga agcagcgata aaaactaaag
    11761 taaatagaat ataaattttt atatgttcat atatccttat attatcgggt atatgggcat
    11821 ttttattata ggtttatact catatatcct tacttttata tacatatctt tatgattaaa
    11881 ggtgttagaa aatgtagtaa attttatact atcagactct atctttatta ctttagtaga
    11941 tacatggttg taaaggttta tagaaaaaaa tataggaagg cagattttta ttgttgcgta
    12001 tatatgagaa tgatataata tgaatattat aaattaataa atttaaattt atggaggtac
    12061 aagagatgat ggaatataaa gttaatgacc aagaacttaa tgcttcaatg tttatcccat
    12121 tcgtcaacag gatatggcca ggagattatg atatggataa aacacagact gcattatcaa
    12181 aaactttgaa tataactgct tatgatgata aagtgcttgt aggatgtctg cgagttcttt
    12241 cagatggtta ttattttgga acaattacag aattgcttgt tcttccagag tatcagaaac
    12301 aaggcattgg tagtaagctt ttccagcttg ctaaagagaa caccccaact atgctatatt
    12361 ttggtgcaca gccaggagta gaagcgttct atgaaaaaaa tgggtgccaa agaagtctgc
    12421 aatcctacat aataaaaaag aaagatagtt attaaagttc cagctttcaa taatcttaaa
    12481 agtacaatat tttagtggaa tcatttcatt ttttattact aatatgacat aaacatgtca
    12541 tattagtatt gatatacttt taatatggtt aagagccaaa gaaaaatata aggagtgatt
    12601 taaaatggaa gaaactaaat tttgccaatg ctgtgcaatg ccaatgggac aaactgatga
    12661 attatatggg acaaataaag atggaagtaa aagtactgat tattgtagtt attgttttaa
    12721 agatggtgag tttacagctg atataagtat ggaagagatg atagaagttt gtatccctca
    12781 tatgcttcaa agtaataagg atatgactga agatgaagca agaacaatga tgaacaattt
    12841 ctttccaaca ttaaaacgct ggaaataatt ttatgcaaaa ttagttatat gcaaaacatg
    12901 aaatatggtg ttgagaataa ggggtggtgt gctttatagc ataccacctg ttttgttact
    12961 tggagttcag ttattttcct aaatatttta aatatactat tttagataag ttccctcttc
    13021 atcttgataa attaatcctt ttttcataag cactccaaga gctcgtttaa aatttttctt
    13081 acttgaatta aataatatag aaatatcttt agggtcagat ttatcattaa atctcatata
    13141 tccatcagaa ccttctaaat aacttaaaat cttattttct aaagtatcaa gttcatcctt
    13201 tctatttcct ctaggagaaa gaccaagttt tccatcttca tatattttta tgacatgtaa
    13261 attttcaaga acatcaccag tttttagttc agtaaagtac tcattatgaa gtattacacc
    13321 agcatattta ttgtctacac atatcattgc agaattatta gtttggaatc catatacaac
    13381 tccagttaca atatctccta cattgtaagt atggtctgtt aataagtatg aatctatgtc
    13441 tgtagtagca gcaattcttt tgcttttgtc taaatatata taaaataaat atttttcgtc
    13501 tctaaatagt ggataagtct tagctttaaa aggaactaat atgtctttag gaagtcccat
    13561 atctataaaa gtaccaatgt ctgtattatc aactactttt aaatgtgcta catccccaac
    13621 cttagctaaa ggcggtatta aagttgcagc agttctgtca tcagaatctt taaatatgaa
    13681 agcatttact tcatcaccta tgtttatttc attttttccg attaatctat tatgaagaag
    13741 tatatcgtct tttgtgttat tagtttgtcc atccaaaaaa tatccaaatt cagtttttct
    13801 ttttactgta agtttattaa aatcacctat gtttatcaaa ttaaaatcat cctctcattt
    13861 tatatttata atttagccat aatatactta tgtagtagtt tgtcacagta tccatattag
    13921 tattagtgaa atttacaata aataatatag ttatttatgt atttttcaat tctctagctt
    13981 cttatatagt ataaactcca atttcttttt tgtctttagg ttttattaaa atttttacaa
    14041 aataagttaa aaagtattga aataaaattt cttttaaagt ataattggta tataccaaaa
    14101 ttggtatata ccatatcgaa aagtggtgac tatatgaaaa tagtaaataa atctagtaat
    14161 ataccattgc atactcaatt aagttcaata ataagagaga tgatagaaac tggtgagctt
    14221 aaagagggcg atgcaataat gccagaaaga gaactttgca acatacaaaa tgtaagtcgt
    14281 atgacagtaa ataaaaccat tgttggatta gttacagaag gacttcttta tagggtacaa
    14341 ggtaaaggga cattcgttgc aaagcaaaaa aagaagtatc agttttcaaa tgtaaaaggt
    14401 tttactgatg ttatgaaaga aaagggagta aacataaaaa cggatatatt atcctttgag
    14461 atggaactac cagatgattt agttaaaaga aagcttggaa taagtgataa tactacaaat
    14521 atatataaga ttgtcagact tagatatact gatggagagc catttggatt agaaatagta
    14581 tatctatcag aagaaatgtg taagggtcta acaaaaggga tattggataa ttcttcatta
    14641 tatagggttt taaatgaaaa atatggctat aagatacaaa aagctgaaca ggtaatggag
    14701 ccagtaatac tttcagatga agaaagtaag cttcttgaaa cagatgaagg tgctttagct
    14761 cttaaacttc atagaaattc ctataataga gaaggtagcc ctatagaata taccatatca
    14821 atatttagaa cagataaata tcaatatgaa atagtattga gtgagtaggt gaataatatg
    14881 aaatgtttaa ttaatggaaa gataatatta aaaaatcaaa tcttagaaaa taaagtatta
    14941 gtatttgatg aaaaaataat tgatatagct gatagtgtac caaaagattg tgaagttata
    15001 gatgctgatg gaaaatatat atctccaggg cttattgata ttcatataca tggaaacatg
    15061 ggaaaagata caatggattc gactgatgaa tctattgaaa caatttcaaa gtcaattatg
    15121 agacatggag taacatcttt tcttccaact acaatgacta tggataaaga acatgtttat
    15181 gatgcacttg aagttattaa aaaagcccaa aatagaaagc ttgaaggggc acaagtatta
    15241 ggagcacatt tagaaggacc ttttataaat gagaattata agggagcaca aaatgaaaaa
    15301 tttataataa attcaaaata cgaatttata aaagaatata aagatgttat aaaagttata
    15361 acttatgctc cagaaaaaga tattgatttt gattttacta gagaaataaa aagatgtaca
    15421 gatatagttt tatctattgg tcatagtaat gcaaattatg accaagctaa agaagctata
    15481 aatttaggag taactaatgt aactcacatg ttcaatgcaa tgactggttt aaaccataga
    15541 gacccaggcg ttgttggtgc agcacttaca actaatgtgt acagtgagtt aatagcagat
    15601 acaatccata taaataaaga tttatttcaa tttatactaa ataataaagg aaaagaaaga
    15661 ctgattttaa taacagattc tatagaagct ggtggtcttg aagatggcaa ttattctctt
    15721 ggaggtcaga aagtcatagt taagggtaat gaagctagac ttgaaaatgg agcattagct
    15781 ggaagtgtgt tgtctttaaa taaaatggta tttaacttct tagataatac aaaccttaaa
    15841 gtgaatgaag caattaattt agcttcttta aatccagcaa catctttggg aataaatgac
    15901 aaaaaaggaa gcttagaaat aggtaaggat gcagatatag ctgtatttga tgagaattta
    15961 gattgtaaaa tgacactttg cttgggggac gtagtatata aaaatatata gtatacaaaa
    16021 caaaccttaa agacagtaaa aacaatataa aaaataataa attaaactta cattagtaaa
    16081 agtagtagtt aaaatttagg aggaataaga tgagagtatt agtgtgtaaa gattatgatg
    16141 gaatgagtaa aaaagccgct gaaatgatag cagctcaaat agttttaaaa ccaaattcaa
    16201 tactgggact agctacagga agtacaccag taggtatgta tagagattta gtaaaaaaat
    16261 acaatgataa tatagtagat ttttcagatg tgatgagctt taatttagat gaatattata
    16321 agttaccaat atctaatgac caaagctatg attattttat gaaagaaaat ctatttaatc
    16381 atgtaaacat aaagccagaa aatactcatc taccaaatgg aatggctgat gatattgaaa
    16441 aagagtgtat gaactatgaa gcttctatag atgcagctgg tggtatagat gtacaagtac
    16501 ttggaatagg tcgtaatgct catataggtt ttaatgaacc tgatactaaa tttgcaaaga
    16561 gaactcatgt agttgaactt acagagtcta caatagaagc taatgcaaga ttctttaaat
    16621 caagagaaga tgttcctaaa aaagctgtaa gtatgggaat aggttcaata ttgaagtcta
    16681 agaaaatatt gcttttagct tcaggagaag aaaaagctga tgcagtatat aatactgttt
    16741 atggagatat aactccagaa gttcctggtt ctatacttca acttcataaa gacactatag
    16801 ttatagtaga tgaagcagca gcaagtaaat tgaatccaaa agattataaa ttagtataag
    16861 aatagaatgt agtttaagta aaaaatagct atcattgagt aatcaaattt atttaatgat
    16921 agctattttt atttaaattt tttacttctt tttatttaaa ttagttatta ttgtttaaat
    16981 tacttgctta ttacttttta cgaaaactcg ttatcaagaa gtatccaaat aagatgttaa
    17041 aaattattat gtttagtggg attctatcaa gcataataaa attacctata attgggatat
    17101 taccaactgg aaacataaag aagaatacag gcatcagtga tagtaaaaaa gatggaaaac
    17161 aaagaagaag taatcttgga atatcaatat accaactacc atcctttttg tattctctaa
    17221 tcaataattc agttcctagt aataaaccaa taccaccaga ggaaattaaa ttaataaaaa
    17281 tgtatctttt aaagtcttgc atataaccag tgtaaaattg agtatagaaa tcataattta
    17341 atttaacagc taaaaaatac ataagcaata gaatgattaa aaataaaaga tatctaagag
    17401 cttttttcat tttaaattct cccttcagaa atactttatt cagttttatt ttgttggaat
    17461 ctgattttat catttagttt aagaaatcca taatattagt gaagaatgaa ataagtatta
    17521 tatatgaata aatattttta gaattaaggt gaattgtagt gcttaatata actataatat
    17581 taattgtttg aatattcaat aaaaagtttg aaaaatatca attttgatat aaactgaata
    17641 aatacagttg aatattaaat tattatttta tcaaaaatat gcaaagtact acaccttcaa
    17701 atatcaccta aagtaattaa aaggcaatta aggtgaagta gttcaagtat ttctaaaata
    17761 agtttaataa attctataat aatttatttt ttgccttaaa attgccttaa aatccaaaca
    17821 attttaaaac taatgtacta taaaaagctg gtataattta aatatagtaa aaaataagat
    17881 ttatcttatc tattgggatt taattattta atcaatatat ttataccatt taatttaaat
    17941 aaaaagtcta ttaaggagtg agcttatgag taagttaatt tatatagcag atgatgaaga
    18001 taatataaga aatttggtaa agacattctt aaaaaatgaa ggtcatgatg ttatggattt
    18061 taaaacgggt gatgaacttt tagaacaatt taatataaag gagtgtgact tagtaatact
    18121 agatataatg atgcctggct caagtggttt tgaagtatgt acaaaacttc gtgagaaaag
    18181 tacagtccca ataatcatgc ttactgcaag agatacagat atagattata ttacagggat
    18241 aaccttaggt agtgatgatt attttacaaa accatttagc ccaatgtctt tggttatgag
    18301 agtaaaatct atatttagaa ggatagagtt tgaaaagaaa caaaattatg ataaatatag
    18361 taactcgata gatatggaat taaaatttgg tgatgtaata ataaataaaa agaataaaat
    18421 cgttacaagt aaaaatgtaa atatagacct tacaccaaat gaatataacc tattgactta
    18481 tttgtttgaa aatatagata gagcagtttc cagagatgaa ttattaaaca aaatatgggg
    18541 atacgatata gaagttgaga caagggctgc tgatgataca gtgaagagat tacgtaaaaa
    18601 aattttaaat acaaatattt taatagaaac tgtatggggg tttggattta ggttgaagga
    18661 gaaaagttaa gatgaagaat ggaaatgaca agaatagaat tggaaagatt aaatccaaat
    18721 ttaaattaaa gttctcaaag aaaaatataa gcataaaaac taggattttg ttgagtatac
    18781 tttgtttgat aattatagta tttatgataa tattagtatc ttttaatgta tttcttgata
    18841 catatataaa aacaactgcg aatgaagaat tgacaaagtc tacagaaata gtagaacata
    18901 tggatagtaa ttttagacct attaagccac cacaagatga tggaaagctt cctcccaaag
    18961 tattatctaa ttttatgaga aatgtacaag ataaagttag gatggcagaa acacaatcag
    19021 atgctgatgc tatggtagtt gattctaagt ataacctgat atttccaaca agagaggatg
    19081 atttcttaaa aaatgttgat gagatggagc tgattaggga gtgtattcaa aatgaaaaat
    19141 tagatttaaa ttcagatgaa aatacacgga tatctaccag aaataaggat tattatattt
    19201 caactgtaaa aattactagt atatatggtg aacaagacaa gtatcttata ctttttatag
    19261 acatatcaaa aactctaaat ctagcacaaa agataaatat tgtattaata tctgtaatgt
    19321 gttttgctgg aatattagca atatttacag ctgtgatttt atctgagaaa attgctaaac
    19381 cgataaaaga gttgtgtgag tttgcaaaaa aaatgggtca aggagatttc aaaagaacct
    19441 actttgactt ttcagataaa gaattagtag agctctcaac tgttatgaat aagagtgctg
    19501 agtatctgga taagtatgat aatgaacaaa aaatattttt tcaaaatgca tctcatgaat
    19561 taaggacacc acttatgtct attaaaggtt atgcagaagc tataaaatat aatgttatag
    19621 atagtaaaca tgcaagtgat ataattcttg aagaaagtga taggttaagt gatatggttg
    19681 aagatttatt atacatttct aaaatagata atataactaa agattttgaa cttgtagaat
    19741 gtgatttaag agaagtactt tcaaattgtg gagctaggca aaatgtaaga gctataaata
    19801 aggggataaa atttatttat gaatttgatg aagaaatggt tttatttgaa tgtgatgaaa
    19861 aaaacatttc caaagcattt atgaatctta tagaaaatgc tttgagatac gctaagagtg
    19921 aaataaaaat agtgtgtaag tataatcaaa aaaatatagt tgtgataata gaagatgatg
    19981 gaattggaat caagaaagaa gatttacctc atgtttttga aagattctat aagggagtag
    20041 ggggaaatca tggaattgga ttatcaatag ttaaatctat tgtaaataag catggtgggc
    20101 gtatatacgt agaaaatggc aaaaaaggag ctaaatttac tatattattt aaattgtaaa
    20161 aaaattagag aaaatataga gatgtttgaa gttgtttttt atatatatct gttgtatact
    20221 gttgaaaaaa gaacaatatt aagctgacat atattacgat agttgacatt taaagtaaat
    20281 gcttaaatat agatacgaaa ggaaaagtaa aatctgaagt gtatttaggt ttaagtaagc
    20341 ttcttatttt ataaaaaata tgtatgaaag aacagctgat ttttaatatt acaaatttag
    20401 taacggttgt atttgaagct tttgtcatcc atatgttttt atcagacttt ttaggtcaga
    20461 aagaagggta tataaaagtt gtaagatatg ctaaactggg ttttataata tgtttaggat
    20521 tctgtaattt aataacttta aaccctaaaa tcacaatgcc tttaattttc atccttattt
    20581 tttcaacatc atttttatat aaaggaaatt taaagacacg attgtttact actgttttat
    20641 taagtatatt ctttatactg tcagaaattg tagttacaag tatttttgtt ttatttgtaa
    20701 aagaaggctt tgaaataatg cttgaaaata atagtataag agtattggcg acaattttat
    20761 ctaaaattgt ctttttgttg acttgtaaaa ttatttgtct atttaaaaag gatgtacatt
    20821 tagatatgcc cataaagtat tggctccctt tatttttaat acctatattt agtttatttc
    20881 tgtctgtttc tatctttgat gtaagtaaat tttttagtct tgaaagttta aaatttctaa
    20941 gcttaatatc tagtgttggt attttatata ttaattttat tgtcttttat ttatttaaat
    21001 ttattataga taaaactaaa ctatctatga aatatgaact acttgaaaaa gaaataattc
    21061 ataaggaaga attaagactt agtaatgagt gttataaaat tattgttgaa cagactgatt
    21121 ctgtcgtttt cgaatggaat atcaaagaaa ataaatcatt tgtatcgcag gcttggactg
    21181 aaaaatttgg atataacaat gcatgtaaga atatttttaa agaaattaaa gataaagact
    21241 tagttcattc tgaagataaa gctatatttg aaggttttct tgaaagtatt aaaaaaaaga
    21301 atatgcacaa tcaagcagta tatcgtttaa aaaaatctaa tggtgaatat atttggtgta
    21361 gaacatcaat tacaagcatt tataatgatg aaaatgaact tttaagagtt gtcggagtaa
    21421 ttgtagatgt agatagtgat ataaaaaaat atgaagagtt aagaactaga gcagaaagtg
    21481 actcattgac caatatatac aataaaggta catttgaaaa attagtggaa gaaactatag
    21541 ttatgaatac aggtgataaa aaagatgcat tattcattat tgatttggat gattttaaag
    21601 aaataaatga taattttggt catccatttg gtgattttgt actaaaaaca tttgctgata
    21661 agatacaaac tagttttggt agtaaagact tagtcggaag aattggtgga gatgaatttg
    21721 ttgtatatat gcaagattat gttacagaag taaatttgca taaaaaagct aaagaattaa
    21781 atagagtact ttcggataat tacactgatt tgagttttag ttttgatgct tctgtcagta
    21841 taggtattgc aaggtatcct caagatggaa cttcattttt tgaattgttt aaaaatgcag
    21901 atagagcact ttatagcata aaagcatcag gtaaaaattc atattgttta tttgaagaag
    21961 aactatatgt acagtgaatt tgaatttaga ttttatgtga gagtaagatt tatatatact
    22021 tgtggctgtc tgatttataa aatatgttac tttgtatatt tttatatgtt tcagtaaggt
    22081 taattaacat gagcaaaggt gtaagtaaat ttatattagt ttaagtttac ttacaccttt
    22141 ataaaaatac aaataataga ttcatttaca ttcttaaatg attaaagctc tttaccttct
    22201 aaacatgcat aacatatcgt tataattcca gtaaataaga ctaaaatgcc cattactaag
    22261 attacaattt catcatctat tactccaaat gtttttaaag taacttattt cgctttgtat
    22321 cataataatt tgtctctttt tacattttaa cttctgctga gatttgagtt agatattcat
    22381 tagaatctcg cacacttata tcatatagaa gatattcttc ataaacaaaa tccccaatac
    22441 gtaaattatt ctgattaaaa aaatttaata acttttcata agccttgtaa atagtttcat
    22501 aactcccttt atgataagca gttacataaa gtccctttgg tttgattgat acaggatatt
    22561 tcttgtactc tttattaact tttgtaaata aatatgaata tgattcaaag tctttattaa
    22621 ttatatcttc acctttaatg attgagccaa tcgtaccata atcactcaat tcatagttgt
    22681 ccataaagtt aatacattca tataaaaaat tttcttcctc atctcctaaa aactgttctt
    22741 tatatgtaac aggtgttttg accaagtact cttcttcatg ttcttttaaa gtaatagtat
    22801 tcgcatctat attacatgca cttttagtaa atgaaatagt agattccatt agagcttgaa
    22861 tattatttag tttttcaatt tcatttttaa tatcaatggt tttcttttct aaaagctcaa
    22921 ttagtaaatt aggtgttctt ttgtctatat aagctttaat ttcttttaaa ggcattttta
    22981 cttccctaag tgaagaaatt ataccaaaaa tgctaagttg atggtatgag tagtatctat
    23041 atccattttc tttctttaat tctggtgaga aaagacctat atcatcataa tggaaaagtg
    23101 tttttttatt gattccacaa atctttgcaa attctccagt tgtaaaataa ttttttgaaa
    23161 cttgactcat aattataatt cctccttgac tatcctgtta cagtatagtt tatcatagta
    23221 ttttgtaaag gagtgtgatt atatgaaaaa attaattcat tttttaaaac catatagagt
    23281 tttaattgtt gtagttttga tatttacgtt tttacaaaca cttggaactt tgtatatacc
    23341 aacacttaca gcaaatatag taaataatgg tgtagtaaag ggagatatag attatattgt
    23401 aaaaacaggt ttaatgatga tgatagtagc tggtataaca gctttatcag ctgtgttagt
    23461 ttgtaaagta tcagcaaatc tttcatctgg attttgtaga gatattagag aagcagtatt
    23521 tataaaatca caagatttat caataaatga ttttaataat ataggtacag catcaatgat
    23581 tactaggagt actagtgata ttactttaat aggtcagtct gtgtttatgt ttattcaatt
    23641 agtattacca gcaccaatta taactgtttc tggtctattc ttagcatatt ctatagataa
    23701 agcaatgaca attattattg ttgtagtaat gtttttattc atgctttcag catttttagt
    23761 tggtaaaaag cttataaagt tgtttaaaat gatgcaaata aagatggata atatgaatag
    23821 ggtgttacgt gaggtagtaa ctggagtaag agttataaga gcttttaata ggagtcgttt
    23881 tgaaaaaaag agatttgata gaactgcaat agattattct gaaacagcaa tttcaataaa
    23941 taaaatattt gcagtactta tgcctattgt aatgttaata atgaatttag gtatagtaag
    24001 tattatttgg tttggtggga tgagagtcag taatggaaat atggaaatag gtcatataat
    24061 ggcacttgtt gaatattgta tcttaatttt attctattta atcatgggag taatggtatt
    24121 tatgtacata cctagagcag gtgcatgtgc agatagagta aatcagatat tagatataga
    24181 acctgaaatt gtagatggaa atggtcataa agatactgtc tcagagagag gacatttagt
    24241 atttaaaaat gtaacctttt catatgcaca atcagaagaa cctgtgttaa ataatataac
    24301 atttgaagca aaatctggag aagtaactgc aattatagga agcactggct caggcaaatc
    24361 tactattgca aatataatac cacgtttttt tgaaattcaa tctggagaaa tatctataaa
    24421 tggtcaggat attaagaaga ttcctcaaaa agaacttaga gataagattg gatttgtacc
    24481 acaaaaagct tttttattta gtggaacaat agaagaaaat ataaggtatg gaaaagaaga
    24541 tgcaagtatt gaagaagtaa agcatgctgc aagtatagct caagctgatg aatttattta
    24601 tgatatggaa gacaagtatg actcatttgt agcacaaggt ggaaataatt tgtcaggtgg
    24661 tcaaaaacag agaatatcca ttgcaagagc gttagttaga aagcctgaag tatatgtctt
    24721 tgatgatagt ttttctgcat tagattttaa aacagataaa agacttagaa aagcgttgaa
    24781 aaatgaaatc aaagattcat cagctataat tatagcacaa cgtataagta caatcatgga
    24841 tgctaatcag attatagttt taaatgatgg aaaaattgta ggtattggaa aacataaaga
    24901 tttacttgaa aattgtgaag tttataagca aattgcggat tcacaactta gtaaagaaga
    24961 acttgcataa aatgaagaat taatctgata tttggaggtg tatgatatga gtgaaaaaag
    25021 aatagatgaa caagatatag aagtacttag ttttgaagat ggcatgtcag cagaaaaagc
    25081 taaggatgca aaaaaaacta ctaagagatt attaaaatat atggcaaaac aaaagtttaa
    25141 aatattaatt atttttattt cagtattgat aagttcagcg cttacagttt tagcacctat
    25201 ggttatggga aaagctattg accaactatt taatggaata aaaactgcag tccaaacagg
    25261 tacaaaattc agtgtgaatt ttagtactat gggagggata gtatcaatac ttctagggtt
    25321 gtacttaatt agtgcagtat ttatatatat tcaacagtat attatgtcag gagttgcaca
    25381 aaatctagtt cttagcatga gaaaagattt aagtgataaa ctcaataaac ttcctcttaa
    25441 gtactatgat tctcataaaa agggggaaac acttagtata gtaacaaatg atttagaaaa
    25501 agtagcagat agtttacaag aaggattaat gcaacttatt acagcagtag ttacagtagt
    25561 tggttctatt gtaatgatga taagtataag tgtaccactt acaatagtat cagctataac
    25621 attacttgtt tcattgggta ttactgttgt aattgcaaga aaatcacaag aaagattctc
    25681 tgaaaatcaa aaggcactag gagagttaaa tagcaatata gaagagatat ttactggtca
    25741 aatagtggtt aaagcattta gtaaagaaaa ggatactata agaaacttta aaatattaaa
    25801 tcaaaatctt tataatgcaa gtagaaaagc acagttttcg tcatatgcaa tatctccaat
    25861 tattagattt ataaacctaa ttggctatgt tattatagca gttgtaggtg ggatatttgc
    25921 atcaactgga gcaatgactc ttggtagtat acaagcgttt attcaatatg taaatcaagc
    25981 atcagagcct actacagaaa tatcttatat tgtaaatatg cttcaagcag caatagcatc
    26041 tgcagagaga gtttttacag ttatggacga ggtggaagag ataaaggatg aagaatcttc
    26101 aaaggttatt agcatgccta aaggtaaagt acaatttgag catgttaaat ttggatatag
    26161 tgatgatttt attcttatga aagatattaa tatcaatcta aatgcaggag ataagattgc
    26221 aattgttgga cctacaggag ctggaaagac aactcttgta aatttactta tgagatttta
    26281 tgagatacaa ggtggaagaa ttactattga tggtgttaat ataaaagatt taaagcgtgg
    26341 agaacttaga actatgtttg gtatggttct tcaagataca tggctgttta atggaagtat
    26401 aaaagaaaat attgcatata gtaagtcaga tgccacaatg gatgaaattg taagagcagc
    26461 aaaatcagca agagtagacc attttataag gactttacca caagggtatg acactatatt
    26521 gactgaggat gcttctaatc tatctcaagg tcaaaagcaa cttctaacaa ttgccagggc
    26581 aatactttca gacccatcag tattgattct tgatgaagca acatctagtg tagatactag
    26641 aacagaggta gaaattcaaa aggcaatgaa taacttgatg aagggaagaa ctagttttgt
    26701 tattgcacat agattatcaa ctattcgaga tgctgattta atattagtaa tgaaagaagg
    26761 aactattatt gaacaaggaa gccataagga acttattgaa aagaaaggat tctatgaaga
    26821 actttataac agtcaattta caagtgaata tgatgaagat gttgtatgat tatcaagttt
    26881 taggcaacta aatgattgat aaatattaag ttaaatatag taacatgtaa ggttatatgg
    26941 aataagagta tttctctatt tcatataacc ttttttattt atcaaaataa gataaaattt
    27001 cctttctaaa ttagattgaa gaatgtattt atcaatataa aagtaaaata atcattatta
    27061 ttagtgaagt aaatattgtt tgccacatat atgccataaa gatgcactta ttttacggag
    27121 atttaacaaa gtagtgtaaa gtatactcta aatataaaat aaatcaaata gagttaaata
    27181 aattgagagg tgatataaat agtgaataaa aaattgaact atagatttga aatgaagcac
    27241 aaaataactg aagcagatgt gttagcttta aaatcaagac tctatccaat aatgaagaaa
    27301 gatgaaaatg catctaaaga tggaaaatat cttattagga gtttatattt tgatactcct
    27361 gaagataaag cacttttgga taaattaaat ggcgttgcaa taagagaaaa gtttaggata
    27421 agattttata ataatgactg ctcttatata aggttagaga aaaaaatcaa acattacaac
    27481 atgacatcaa agctatcaac taatttaacc aaaaaagaag taataaatat actaaataat
    27541 gatattgaat ttctaaaaga gtcgacaaat tgtttattaa gagaatttta tttgaaatta
    27601 aaatgtgaaa gattagaggc aaaaagcata gttgattaca atagagaagc ttttatttat
    27661 ccagttggaa atgtaagagt tacaatcgat agtgatatta aaacttctgt aaattcggta
    27721 gatttattta ataaagattt gccaactatt tcggtaattg atgagaatat gactgtattg
    27781 gaggttaaat atgatgaatt tataccagat tttataaagg atttaattca aattaataag
    27841 actacctcta cagcagtttc aaagtatgct tcaagtagat tatatattta agaataaagg
    27901 ctatgattta tataaagata tacataaata aggttaaaaa acaaataagt taaaaataaa
    27961 taaaataaaa aataaataag ttaaaaatag gaaggatgat tttaattatg acatttaatg
    28021 atatttttaa atcaagtttt atagaaaacg tatcaggttt ttcatttgtg gacagtgctt
    28081 tagcactagg ttcagctttt ttagtaggtt tgtttatata tatggtctat aaaaagactt
    28141 atatgggaat tatgtattct cgaccattca atgtgtcgct tgtagcactt acaatgctta
    28201 caacttttgt aatattggcg gttacttcaa atgtagtttt atccttaggt atggttggtg
    28261 ctttatctat agtaagattt agaacagcaa ttaaagaccc aatggattta gtatttctat
    28321 tttggtcatt aggttcaggt atagttttag gagcaggttt gattccctta gctgtcatgg
    28381 gttcaataat tatgggattg attctaatat tcttttcaaa taaaacaata tctgaaactc
    28441 catatatatt gatggtaaat tgtaataatg aagattcaga agatatagca actgataaaa
    28501 ttaaaaaagt atttactaaa tatcaaataa aatcaaaaag tgttacacca gaaaaaggaa
    28561 tagaattagt gtttgaagta agaatgaaag atggagaaac aagtcttata aatgacttga
    28621 gtcaagtaga tggagttact aatgcagttc ttgtaagtta taatggtgac tatgtagcat
    28681 agagaggagt aatcttttat gaaagataaa aaatttaccc ttcttatctc gattatgatt
    28741 atatttttat gtgctgtagt tggagtttat agtacatcta gcaacaaaag tgctgattta
    28801 tatagtgatg tatatattga aaaatatttt aacagagaca aggttatgga agttaatata
    28861 gagatagatg aaagtgactt gaaggatatg aatgaaaatg ctataaaaga agaatttaag
    28921 gttgcaaaag taactgtaga tggagataca tatggaaacg taggtataag aactaaagga
    28981 aattcaagtc ttatatctgt agcaaatagt gatagtgata gatacagcta taagattaat
    29041 tttgataagt ataatactag tcaaagtatg gaagggctta ctcaattaaa tcttaataac
    29101 tgttactctg acccatctta tatgagagag tttttaacat atagtatttg cgaggaaatg
    29161 ggattagcga ctccagaatt tgcatatgct aaagtctcta taaatggcga atatcatggt
    29221 ttgtatttgg cagtagaagg attaaaagag tcttatcttg aaaataattt tggtaatgta
    29281 actggagact tatataagtc agatgaagga agctcgttgc aatataaagg agatgaccca
    29341 gaaagttact caaacttaat cgttgaaagt gataaaaaga cagctgattg gtctaaaatc
    29401 acaaaactat taaaatcttt ggatacaggt gaagatattg aaaaatatct tgatgtagat
    29461 tctgtcctta aaaatatagc aataaataca gctttattaa accttgatag ctatcaaggg
    29521 agttttgccc ataactatta tttatatgag caagatggag tattttctat gttaccatgg
    29581 gattttaata tgtcatttgg tggatttagt ggttttggtg gaggtagtca atctatagca
    29641 attgatgaac ctacgacagg taatttagaa gacagacctc tcatatcctc gttattaaaa
    29701 aatgagacat acaaaacaaa ataccataaa tatctggaag agatagtaac aaaataccta
    29761 gattcagact atttagagaa tatgacaaca aaattgcatg acatgatagc atcatatgta
    29821 aaagaagacc caacagcatt ttatacttat gaagaatttg aaaaaaatat aacatcttca
    29881 attgaagatt ctagtgataa taagggattt ggtaataaag ggtttgacaa caataactct
    29941 aataacagtg attctaataa taattctaat agtgaaaata agcgctctgg aaatcaaagt
    30001 gatgaaaaag aagttaatgc tgaattaaca tcaagcgtag tcaaagctaa tacagataat
    30061 gaaactaaaa ataaaactac aaatgatagt gaaagtaaga ataatacaga taaagataaa
    30121 agtggaaatg ataataatca aaagctagaa ggtcctatgg gtaaaggagg taagtcaata
    30181 ccaggggttt tggaagttgc agaagatatg agtaaaacta taaaatctca attaagtgga
    30241 gaaacttctt cgacaaagca aaactctggt gatgaaagtt caagtggaat taaaggtagt
    30301 gaaaagtttg atgaggatat gagtggtatg ccagaaccac ctgagggaat ggatggtaaa
    30361 atgccaccag gaatgggtaa tatggataag ggagatatga atggtaaaaa tggcaatatg
    30421 aatatggata gaaatcaaga taatccaaga gaagctggag gttttggcaa tagaggagga
    30481 ggctctgtga gtaaaacaac aacatacttc aaattaattt taggtggagc ttcaatgata
    30541 ataatgtcga ttatgttagt tggtgtatca agggtaaaga gaagaagatt tataaagtca
    30601 aaataagatt aaaattgaat ataaataatt ttaatactgt taagtctaaa tatttactta
    30661 gcagtatttt tttagaaaat aatgtatttg gttaataata taaggtaata aattgataa
//
LOCUS       DAMJZE010000027        30706 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJZE010000027 DAMJZE010000000
VERSION     DAMJZE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.027"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            181..2082
                     /locus_tag="PC541_001149"
     CDS             181..2082
                     /locus_tag="PC541_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2838620.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2085..3128
                     /gene="ypdE"
                     /locus_tag="PC541_001150"
     CDS             2085..3128
                     /gene="ypdE"
                     /locus_tag="PC541_001150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2838621.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3143..3448
                     /locus_tag="PC541_001151"
     CDS             3143..3448
                     /locus_tag="PC541_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2838622.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3545..3880
                     /locus_tag="PC541_001152"
     CDS             3545..3880
                     /locus_tag="PC541_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838623.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3912..5234
                     /locus_tag="PC541_001153"
     CDS             3912..5234
                     /locus_tag="PC541_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2838624.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            5244..6314
                     /locus_tag="PC541_001154"
     CDS             5244..6314
                     /locus_tag="PC541_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2838625.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6319..7506
                     /locus_tag="PC541_001155"
     CDS             6319..7506
                     /locus_tag="PC541_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2838626.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7703..8575)
                     /locus_tag="PC541_001156"
     CDS             complement(7703..8575)
                     /locus_tag="PC541_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2838627.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8969..9160)
                     /locus_tag="PC541_001157"
     CDS             complement(8969..9160)
                     /locus_tag="PC541_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838628.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9380..10426
                     /gene="cobT"
                     /locus_tag="PC541_001158"
     CDS             9380..10426
                     /gene="cobT"
                     /locus_tag="PC541_001158"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2838629.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10458..11018
                     /gene="cobU"
                     /locus_tag="PC541_001159"
     CDS             10458..11018
                     /gene="cobU"
                     /locus_tag="PC541_001159"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2838630.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            11028..11801
                     /gene="cobS"
                     /locus_tag="PC541_001160"
     CDS             11028..11801
                     /gene="cobS"
                     /locus_tag="PC541_001160"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2838631.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11821..12435
                     /locus_tag="PC541_001161"
     CDS             11821..12435
                     /locus_tag="PC541_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2838632.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12502..12681
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12811..14310
                     /locus_tag="PC541_001162"
     CDS             12811..14310
                     /locus_tag="PC541_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2838633.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14349..15725
                     /locus_tag="PC541_001163"
     CDS             14349..15725
                     /locus_tag="PC541_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2838634.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15726..16697
                     /locus_tag="PC541_001164"
     CDS             15726..16697
                     /locus_tag="PC541_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2838635.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16729..17799
                     /locus_tag="PC541_001165"
     CDS             16729..17799
                     /locus_tag="PC541_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2838636.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17801..18688
                     /locus_tag="PC541_001166"
     CDS             17801..18688
                     /locus_tag="PC541_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2838637.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18676..19308
                     /locus_tag="PC541_001167"
     CDS             18676..19308
                     /locus_tag="PC541_001167"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2838638.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19317..20543
                     /locus_tag="PC541_001168"
     CDS             19317..20543
                     /locus_tag="PC541_001168"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2838639.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20540..21148
                     /locus_tag="PC541_001169"
     CDS             20540..21148
                     /locus_tag="PC541_001169"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2838640.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21138..21713
                     /locus_tag="PC541_001170"
     CDS             21138..21713
                     /locus_tag="PC541_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2838641.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21706..22458
                     /locus_tag="PC541_001171"
     CDS             21706..22458
                     /locus_tag="PC541_001171"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2838642.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22442..23563
                     /gene="cbiG"
                     /locus_tag="PC541_001172"
     CDS             22442..23563
                     /gene="cbiG"
                     /locus_tag="PC541_001172"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2838643.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23560..24285
                     /gene="cobJ"
                     /locus_tag="PC541_001173"
     CDS             23560..24285
                     /gene="cobJ"
                     /locus_tag="PC541_001173"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2838644.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24282..25034
                     /gene="cobK"
                     /locus_tag="PC541_001174"
     CDS             24282..25034
                     /gene="cobK"
                     /locus_tag="PC541_001174"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2838645.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25053..26531
                     /locus_tag="PC541_001175"
     CDS             25053..26531
                     /locus_tag="PC541_001175"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2838646.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26561..27466
                     /gene="hemC"
                     /locus_tag="PC541_001176"
     CDS             26561..27466
                     /gene="hemC"
                     /locus_tag="PC541_001176"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2838647.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27456..28955
                     /gene="cobA"
                     /locus_tag="PC541_001177"
     CDS             27456..28955
                     /gene="cobA"
                     /locus_tag="PC541_001177"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2838648.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28972..29937
                     /gene="hemB"
                     /locus_tag="PC541_001178"
     CDS             28972..29937
                     /gene="hemB"
                     /locus_tag="PC541_001178"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2838649.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30090..30668
                     /locus_tag="PC541_001179"
     CDS             30090..30668
                     /locus_tag="PC541_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2838650.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12438 a         3064 c         5671 g         9533 t
ORIGIN      
        1 ttaaaatata ctaaaaattg aactatatat tttattatta taaaatattt atattaaatt
       61 gtggaattaa aatatactag gatataaaat tcttaaataa aaagaaaagg ttgtttttat
      121 aaaaaatatt ttttatacca ctttaaaaaa cacaataata acactgaggg aggtggggat
      181 atgagagaac gcattattct tgaaacatta aaaaatagta gtggttatct aaacatagat
      241 tattttgcag ataagttggg ggtatctact cgaactatca gaaatgaaat aaaaaagata
      301 gaaactataa aagaatgtaa tggttttaaa ttagaatata agtctaaatt aggatatatt
      361 ctaaatatta aagaccaaga taaattcgag cattatctaa gaaacttacc ctcagattta
      421 gttgaaaatc cagaacaacg gttggagtct ataatagtag agcttttagt aaatgaaggt
      481 tataaaacta tagaacagtt aagtaaaaaa tttctagtaa gttcatccca aataaaaaat
      541 gatttaaaaa agatagatga aaagataaag gatacagaat taaaactaga gcgaaaggct
      601 cattatggaa ttaaaataga aggttctata aaagaaattc aaagtatatt ggtggatagt
      661 tattttagag ggaatagaaa tataacagag tacaggaata agtttataga taatttaaaa
      721 ttagctaata ttagaagtac aataaaaaat gtacttaatg aacatgattt agaagcaaat
      781 ttaacagaac ttgaggaaat attagcacaa attattatat tgtatataag agttagtatg
      841 agagtagtgg gaaatgctag cgatttaaaa ttatatacag aagatttaat tatagataaa
      901 ttattagata aaatttttag agataaaaaa tataacctta attatgatga aaagtattat
      961 ttaaaggagc ttataaaact aaagactaaa gataagaaag ccactataaa gaatattgat
     1021 aagaataaac ttcaagatat tatgttcgaa ttttttagag agatagataa aaaatataac
     1081 tctaattttt tagaagataa agagtttttt aacttatttt acttacatat tgcatgttta
     1141 atagaacgta taaagaagaa ccacaagata ataaatcctt tttcagttaa aataagtcaa
     1201 cagtatccaa ctgtttttaa tttatctatt caattttcaa aaatcataga aaatgagtat
     1261 catataaaaa taagtcaaga tgaaataggg tttatagcta ctcacattgc tgttcctttt
     1321 gaaaagagag aagaagctaa ttttaataaa aaatataaaa tagcaataat atgctcttct
     1381 ggtggaggta gtgcattttt gataaattta agacttaaag aaatttttcc aaatgctgag
     1441 ataagaaatt tttcattact agaagaaaaa gctgtaatag aatttgcacc agatttgatt
     1501 ttttcaataa caaatctttt atttgaaata aatgctccag ttatacttat aaatgaaatt
     1561 ttagacgaat tggattattt agatattaaa gaaagtgtta gatttgcaga ccatatagga
     1621 aatatatcaa gcccaaaaca atatatatta ggtctatttg ataagaatca ctttaggtgt
     1681 ataaaagata aagctaagta taaggatatc cttgatagta tgtcaaaatg tatagttgac
     1741 gagggagctt gttctccaac ctatcccaaa gatgtatggg aaagagaaag ttatttgagt
     1801 actatttata caaatggggt ggctatacct catccaatcg agatgacagg aaacaaaaat
     1861 ataatatcag tagctttgat acaaactgat attatttatg aaaatagggt gcctaaaatt
     1921 attttcatga tttcattaat aaagggaaat ttagaacttc ataagcaaat atctaaatat
     1981 ttaagtaaga ttatggcaaa taaggatatg gtagacatgc taaataagtc acaatcttat
     2041 gaagagttta tgtataagtt aaaaatctat ctgggaggtt agacatgaat atagagtttt
     2101 ttaagaggtt gtcagaagca gatggtatag catctaatga agaagaggtt agacatgttt
     2161 tattggaaga actaaaagct tatagtgata aaattatctg tgatgggcta gggagtatca
     2221 tcttttctaa aataaaagat gagagtgcac caaacgtaat gatttgtgct catatggatg
     2281 aagttggatt tatggtgaga agtatagaca aacttggtat gattcactta ataacaatag
     2341 gaggagtaaa acctcttgca cagtttgtac aaaaagttag aattacaaca aaagaaggga
     2401 agaaaatccc cgctgtcata aatgctacat ataataatgg taaggctgaa aatatatatg
     2461 cagatattgg agcatataca gaagaagatg tatataacct agggattaat gttggggata
     2521 tggtaacata tacaacaagc tttgaggaat ttactttacc agaccgatta gtgggaaagg
     2581 cttttgatga taggatagga tgttttgtga tgggagaagt tttaaaagaa cttaggaaag
     2641 aaaatctaaa ttgtaatatt cactttgcgg ctacaagtag tgaagaggtt ggaataaggg
     2701 gagcaaagac atcaacacag ttaataaatc cagatatagt atttgtaata gatgtagcat
     2761 gtgctaaaaa tgagtttgtg agagactata caaataagaa acaaattgat aaaggtgtaa
     2821 tgctaatgca tagagatagg actctagtcc ctagtagaaa aatgatagat tatgtaatgg
     2881 aaattgctga taaaaataat ataccacttc aacatgatat gtttgaatca ggtgggactg
     2941 atggaggaga agcacatctc gtaaatgaag ggaaaccttg tgtagtaact tgtgtaccag
     3001 ttagatatgg tcattgtgca ttttcaatag taagtaataa agatttggag aacattataa
     3061 gattgtatac acagttaatt ttaaattttg atgaagaaca atataaacat ataagaaatt
     3121 ttatctaaag gagaataaga aaatgtcaac agaagtacaa ataatggaac ttatatcaac
     3181 tgcaggagag agtaaatcga aagcttttga ggctttaaaa aaagtgaagt caaaagattt
     3241 taaaggagca aaattgttaa tagaggaggc tcgtaaaata gatattgaag ctcacaggat
     3301 acaaacagaa ttaataactc aagatttaaa tgaagatgtt gaaagtccag caatgagtct
     3361 tttaatggta catgctcaag accactatat gacttctcag ctagcaaaag acttgataga
     3421 ggctttgata tattgtcttg ataagtaatt gtaatataga aatagattat ttatgtaaaa
     3481 attcttttaa aagtttagat gcttttaaga gataataaaa atacaaactt taggaggaat
     3541 tattatgaaa atattattat gttgtgcagg tggattttct actaatatgt taatgcaaaa
     3601 tatgaaaaag gtggtaaaag agagtgctaa actcaatgaa aaagattttg agtttacagc
     3661 tataccagca gattcaatag aaagtgaagt tgatgattgg gatatagtac ttataggtcc
     3721 tcaaatagct cacaaaatag acttcttaaa aggaatatta gagccaaaaa ataagccttt
     3781 tgcagtaata gataaagatg tatatggaaa aatggatgga gctacagtta tgaagttggc
     3841 attaataact tataagaaac atcaattagc tcaaggataa taaaaataat gagggaaaag
     3901 ggagattaga tatgttttca aaatttgaag catttatgaa tagagtattt atgcctttag
     3961 ctcataaggt tgacaatcaa cgtcaccttg gttctataaa ggctggtatg gttgctatga
     4021 caccattcac aatattagga agtatatttg caatacttcc agcacttcca aatatgcttg
     4081 gagaaaataa tccaataagc aaattcatac ttagcaattc agcaatattg gatttaccag
     4141 taagtctttc cataggttta ataggattat atgcttgtat gtgtattgct tttaatctgg
     4201 ggaactacta taaattatat ataccaggtt gtgttgcact atcaactttt tcttttttat
     4261 tcctagtagc ctcatttaca ccagatggag ctttagaaat aggaaattta ggagctaaag
     4321 ggctatttac aggtatgata gcagggcttg gttctgtgga gttatataat ttttgtaata
     4381 agaagaatat cacaataaaa atgccagaaa atgtacctga ttttgtatct aggtcttttg
     4441 aattaatacc tactactata atagtttgtg gaacttttat agttgctcgt tttatttcat
     4501 taaaagtatt tggagaatta cctccacaga tacttactca atttttatct ccattagtag
     4561 gaagtatgga taacccatgg tcagttcttg cacttaactt tgctatatgt actatattct
     4621 tttttggaat acattcatca gtatttagtc ctataactag acctataatg attacattta
     4681 tagctgaaaa catagcagct atgcaggctg gagaaacaat acctcatttc tttacagcag
     4741 gagcttcaag tgcattcttt ggatttacag gatgtggaat tagtataggt tgtgtaattg
     4801 catgtatgtt atcaaaaaat gcttcctata agaagattgg acgtgtatca ttattccctg
     4861 cattatttgg gataaatgaa ccaatattat ttggagcacc gataatatta aatccaataa
     4921 tgtttatacc acatgtattt ggtggagcta taataggtac actaccaatg ttttttatgc
     4981 actggggatg gttagcaaag ccagtatttg accctccata tgtaggggta ttcttagaag
     5041 gatttttaac taatggagat tggcattcta tattagcaaa tgcattgcaa ttagtattag
     5101 cagttttatt atattggcca ttctttaaaa taatggaacg tcaacaaaat aataaaaaac
     5161 aaaaagaaga aactaagaat atattctcta aagaagaaga aggcttattg gatgatttag
     5221 gattggattt ctaatatttt gttatgaata atacaatgga gagattagta tccttaatct
     5281 ataaaagagg tgttgacgct cttttgatta aagcaaaaaa tactaaaaga tatataggag
     5341 ctttatcagg tagtggtgta tatgtgcttg ttactaagga taaaaattat caaattctag
     5401 atggcagata tattgatgaa gcagataaaa aaacgactgg tttcataaaa aggattgtat
     5461 ctcaaggttc ttatatacca actataattg agttattaaa agaattaaat ataaaaaagt
     5521 tagcaataga aaaccaagct atgagtatac aagagtatct ttcactaaaa aatgaaggtt
     5581 ttgaaatcaa tttaataaca aatgaattat ggaagctaag ggctataaaa tctaaagaag
     5641 aaattaaact tattaaaaag gcttgtgaga ttacagatga agtatttgat gaggttattt
     5701 ctgaaattaa aaaagatatg actgagctag aagtatcagc tctaattcag tatcatgcat
     5761 taaaaaaagg tgcaagtgga atgtcttttg aaacaatagt ggtatcagga gaaagaggtg
     5821 caatgccaca tggaagacct acaaacaaaa aattgaaagt gaatgaagct ataacaatag
     5881 attttggtgt tgtttatcaa gggtatcaat cagacatgac tcgtacaata tctataggca
     5941 aacctcctaa aattattaaa gagatatatg atgtagtatt ggaagcacaa ttatctgcaa
     6001 tagagtcaat aaaggaagga actagggcaa gtgatgttga caaagtggct agaaaaatta
     6061 tagataaaca tggatttggc gaatatttta atcatggact gggtcatggt ataggtcttg
     6121 gagatggtga agttcccact ttaaatccaa atagtgaaga tatattagtg gaaggaatgg
     6181 taatgtcttg tgagccaggt atttatattc caaatgtagg gggagttaga atagaagatg
     6241 atattgttat aatagatgga aaaggtgttc cactcaataa gacatccaaa gagtttataa
     6301 tattaggaga gtaaaattat gaaatataat tttaataaag tattaaatcg taaagaagga
     6361 aattgtagaa aatggagcaa ccatgtgatt aaagaaaaat ttggcttaaa tgaagatgca
     6421 attcctatgg atttagctga tatcgatttt gagtgtgctc ctgctataaa agaatctatc
     6481 ttaaaaagag catcagtagg agattatagt tatactttta taggagatga cttctatgat
     6541 gctattataa attggaataa aagaagattt gatgtatata tagaaaagga ttggataaaa
     6601 ttaacttttg gtactgtttc aacccttcat tatatagtcc aagcatatac taaagaagga
     6661 gagggtgtat tgataaatac acctgcttat gacccttttg cagaggctgt agtaaataat
     6721 aatagaaagt tgtgttgtag ctcattaaaa ttagataaca atagatatta tttagattta
     6781 gaggatatgg aaaatcaaat gaaatacgag aatataagat tgcttatatt ctgtagtcca
     6841 caaaatccat caggtcgtat atggacaaaa gaagaactat atcaagtgtc agagttatgt
     6901 ttaaagtata atgtaatttt ggtaagtgat gaaattcata gagatataat atttaaagat
     6961 tatagatttg tatctttgtg gaatgctcat ccagaaattt ataaaaatag tataatctgt
     7021 gtatctccta ataaaggctt caacttaggt ggattaaaaa catcatatgt attaataaga
     7081 gatgtaaaaa ttagggagac tttattagaa aggctaaaaa gtaattcaat aacttctcca
     7141 aatgtatttg caataccagc aatagttgca gcatataatg aaagcgaaga atggctagat
     7201 gctatgacta gttatgtaga ggagaatttt gaaatagttt atgatttctt tgaaactaat
     7261 ataccaaaag ctaaagtgat gaagtcagac tcttcttttt tagcctggat agatgtaaga
     7321 gaagtattta gaaatgaaga agaatctaaa gaatttttta gacatgcaaa cttaactatg
     7381 gtagttggaa gttattttgt gaaagatgga gatggattta ttcgtattaa tgtggggtgt
     7441 cctagagaaa cattaaatga ggctctaaat agaatcaaaa aaacgtatat aagtatgtat
     7501 tgttaaatca taaaaaaagt atctatggtt tatataaaaa cacattattt agttatcaaa
     7561 tattactgga attttgtatt ccagtaatat tttttgatta aggaatatta ttgaggaata
     7621 ttaaataata ttatttcata cccttatatt aacttatata aaaatatttt attattttgg
     7681 gaacgggtta gctaaccttt atctagtgta tatttatatt gtaagaagca gtaaaaactt
     7741 ttcctttact aagtttatta ataaacttct tatctttata aattttgtta gaatttatac
     7801 tatcagcaag accataccaa ggttctatac ataaaaaagg agcagtagaa ttagtttcac
     7861 tataataagg agtccaaata ccaactagag ggaaatcttc catagatata gttacatatt
     7921 tactaccatc attattacat atagacactt catctatgtt ggtgtatatc aaagcatcat
     7981 tcttgaatac ttctggattt aactgtaagt tttttaattt tccaataggt attatatcat
     8041 ctgtaaaaga accatttaaa ttgatttttt ctacatcatt tttactttta aactcaaggt
     8101 agtgtttaga aaaatcattt tgttcgtaaa aaggaaggtt gaatgcagga tgtccaccta
     8161 tagaaaagaa tatatcttta gaatcagtat tttttacaat ccattctacc tcaatactat
     8221 tatcatttaa tgtatagttt atgattagct caaaagaata tggatacttc tttaaagtag
     8281 attcattatc tgttaattta taagttacaa atgtatctcc tttatctaca atctcaaagt
     8341 ccatatctct agcaaaccca tgttgagtca tgctatataa attttcttct attattgttt
     8401 cattatcaaa aagtttacct actataggga acaatatagg tgattgtcta ccccaatatt
     8461 ttttattacc atcccaaaga atgtttttgt cgtatttttt agaaaatatt ttagttagtt
     8521 cagcaccaaa actgcttgat tcgataatta aattttcatt ttgtaatata ttcatttaaa
     8581 atctccttta ttatatttta tgagtatatt atactataaa gtataaagat tattaaattt
     8641 tatttgatat tatatttgaa aggtagattt tagtatattt tgaaaatttt gttttgaaaa
     8701 tttgggatta tttaagaaat tttgtattat aaatagaagg aaatagataa attgtataga
     8761 ataaaatata ttaagataag ttaataatgt taaatgggaa gtgagtttat ggaaaaacca
     8821 aatacaaaag ttgttaactt tcaagagtat aaagacagaa aaaaccaaag agaagtaaat
     8881 agagacgaac ttttaaagct tataaaaaaa attgtgactt caaaataggg agttgttaaa
     8941 ctccctattt taatgggttt cataataatt aagacattct aagtccatct atataatctt
     9001 tgaattgtga gaaatctgat tcactactta attctatggt ttctgaacct tccgtataaa
     9061 aattcttaag atagatagta taattattat caacacaatc atgataaata catctatatc
     9121 catcttcata caaacttttt aacttagaaa aatcatccac aatatcatct ccttaaagtt
     9181 cttttaaata tatttttaca aaaagcatct aattgtatgc aaattgacaa tgtaaaataa
     9241 aggaaatgaa cataaaatgt agaataattt ttatatgtat gtaaaaataa aaagaaaaag
     9301 ttgaaatttt tagaaaataa gagtattttt tatctaaaaa gagagtttaa aaaataaaaa
     9361 aatgttggga ggataaaaaa tgagtttatt agaaagtata tcaaaaaaca tatattccct
     9421 agataactct tctattgaaa aaacaaagca gagattggat aggcttatac atccaactgg
     9481 aagcttagga aagatagaag atatttgcat gcaattagca ggaatatttg gcaatgaaaa
     9541 ttttgacaca agtaaaaagg ttataattgc ttttgcggga gaccatggtg tatatgaaga
     9601 aggcgtagca cctgaccctc aaaatataac aaaattacag tttccaaatt tttcgaaagg
     9661 tctatgcgga gttggagtaa taagtaaatt tgtaggggca gatgttgtag ctgtagatgt
     9721 aggaataaac tgtgatgaaa aactggatgg ggtattagac tacaagataa gaaaagggac
     9781 atcaaatatg gcaaaaggtc cagctatgag taaacaagaa gcaattagat gtttagagat
     9841 aggtattgaa atagcagagc agtgtataga aaaagattat aaggttattg gtattgggga
     9901 gatgggtata gcaaatacta ctccaagtac agcaataata tctgtaattt cagggtgtga
     9961 cccgttagaa gttactggta taggtgcagg tcttaagaaa gaaagactaa agcataaggc
    10021 tgaagtaatt agaaaggcta ttgaaattaa taatccaaat ccaactgatg gagtagatat
    10081 tttatctaaa gtaggagggt ttgaaatagg ctcaatggca ggtgttatat taggttgtag
    10141 tgctaatagg attccagtag taattgatgg gtttatttct tatgcagcag cattaattgc
    10201 atataagata aatccaaaaa ctagagaata tatgatagca tctcatttat cagcagaatc
    10261 aggaactaaa agagcattag acatattaaa attagaccct ctattaaata tggatatgag
    10321 attaggtgag ggaagtggag ctgctttagc atttgatata atagaagctt caaactatac
    10381 ttataaaaat atggcaactt ttgatgaaat tgacatggga agataaatat aaaaatttaa
    10441 tatgtaggag tgttttgatg agtaaaatta ttctagtaac aggaggagct aggtcaggga
    10501 agagtaactt tgcagaaaaa ctatgttttg acagaaataa taatacagct tatatagcaa
    10561 cctctattcc atttgatgat gaaatgaaag atagagtaaa aaaacatcaa gagaatagac
    10621 ctagaaactg gaatacttat gaaatatatg aagatatata ttccataatt aaggagatat
    10681 ataaagaaca tgaaactgtt atactagatt gtgtaacatt attagtaaat aatctaatgt
    10741 ttgaacacaa tctaaatata gaagaatcta gtcaagaaga aataaacaaa ttagaacaat
    10801 acataaaaga acaagttgtc aaacttattg aagaaataga aaaaacaaat ctatactttg
    10861 tatttgtaac gaatgagtta ggtatgggaa ttgttcctgg gaataaactt agtagaattt
    10921 atgctgatat tgtaggtcgt ataaatcaat atatagcttc gaaaagtaat gaagtttatt
    10981 ttgtagttag tggcatagct atgaagataa aggagtagta tgcaattatg aaaagattta
    11041 ttttaatact acagtttttg acaaggatac ctataaaatt aaatgtagga tttgatgatg
    11101 aattttataa gtcaatagta tattttccac ttgtaggttt tgtcataggt atattatcct
    11161 atttaatagg atggatttct atgcttttat ttgaaccatt tatagcttct ataataataa
    11221 ctctagctgg tgtcttaatt actggaggac ttcatataga tggattagga gatacatttg
    11281 atgcaatata tagttatagg gataaagaaa agatgcttga aataatgaaa gattcaagat
    11341 taggtacaaa ttcacttcta gcaattatgt ttgtactttt attaaaggtt ggattcgttt
    11401 acgatataat aagtaataat tcattgtggg ttataatttt tatgccaatg attgcaaggt
    11461 taggagtaat gttattgaca tataaaactg tgacaccaag agaaaaagga atgggaaatt
    11521 tatttatagg aaaattaact acaagtatgt taataactgc tataatatac acattattga
    11581 tagttgctct tattactaaa tttatatttt tattacctaa tatagtgttg attaaagtat
    11641 taggttcgat tatagtggtt tttgtattta taatattatt caaaaaacat atttataaaa
    11701 aaattgacgg agtaacagga gatatattag gctgtggaat agaactttca gagcttgtat
    11761 atttaattta catatatttg ttaattttta tgttttttta aagtaaaaag tggtgataat
    11821 atgataagat taatattaat aagacatgca ctgacaaatg ataataaaaa aggaagatta
    11881 tcaggccata taaatagttg tattagtgaa gagggcaaac tacaaataaa taaaattacc
    11941 agatatctga gtaatgaaaa catagataag gtttatacaa caccatctac acgtactaaa
    12001 gatacagtag aaaaaatatc aaaattgaaa ttattagaaa tagaagaaaa agaagcttta
    12061 agagaaatta gttttggaga ctttgaagga agaacttttg aagaaattaa aatcaaatat
    12121 ccaaatgagt ttgaaaaaat gataaaagaa ggtaacaact atagatatcc aaatggagaa
    12181 agtttgatag actcttataa aagagtggca aaagaaattg ataatataat tttagaaaat
    12241 aatagtaatt tagatacaaa gacaatactt atatgttctc atgcaggaac aataagaaat
    12301 ataataacac atttgatttc tggtagttat aagtatcact ggaactttaa aattgataat
    12361 gcatctatta cagttttaga aattgatggt ggatttgctg taatagataa aatgaatttt
    12421 acagacttca tataatttaa tcaaaaccaa acattacaat ctgtgtttgt aatatatctg
    12481 taaaattagc aatgaaaata gaatattaga ttaaaattag gttcttaatt agatgttaaa
    12541 agggaaaaag gttagatgcc tttgcagccc ccgctactgt gaaaccaacg aaactataaa
    12601 taccactgtc agtttgatgg gaaggttata gaagtaggat gaagttaagc caggatacct
    12661 gcctaattta atttaaaaca agaggtcttc ggggtgaaag atttttaatt aatatatata
    12721 aaagtcctat ctttagaagt attaggactt tttttaattt gttttttagt aaattttaac
    12781 aaggagggat ggaaagtggg gaaaaaaata atgctacaag gaacagcttc aaatgtaggt
    12841 aaaagtgtct tagtagcagg tttgtgtaga atatttaagc aagatggtta tactgttgct
    12901 ccttttaagt cacaaaatat ggctttaaat tcttttataa ctaaagaagg attagaaatg
    12961 ggaagagcac aagtatttca agcagaggct gcgaaaatag agccaatagc tgatatgaat
    13021 ccagttttat taaaaccttc tggaaataat aaaagtcaag ttattgtaag aggtaaagtt
    13081 gtaggagaaa tgccttcttc taagtatcat gattataaat tggagttagt agatgtgttg
    13141 aaggaaacat ttaatagctt aaattcaagg tatgatattg tagttatgga aggggcagga
    13201 agtaatgcag agataaactt aaaagataga gatatctcta atatgggtat ggcagaaata
    13261 gctgatgcac ctgtaataat agtcggagat attgatagag gaggcgtatt tgcctcatta
    13321 gcaggaacaa tgcttttatt aaatgaagat gagaaaagaa gagtaaaagg tgtaataatc
    13381 aataaattca gaggaaaaaa agagctttta caagatggtg taaagatgct agaagacatc
    13441 ataaaagtac ctgttatagg agtagtacct tatacagata taaaaataga agatgaagac
    13501 agtgtaacta cgagatttaa gaaaaaaatg aataaaagtg atatacacat agaaataatt
    13561 agaaccccac atatgtcaaa ttttacagat tttaatatat ttgagacaca agaagatgta
    13621 agtataagat atgtagacta tggagaatct ttaggaaatc cagatatatt aataatacct
    13681 ggaactaaaa gtactataga cgatttaaca tatataagaa aaagtggtct tgaatcgcaa
    13741 atcaaaaatt tacatagtca aggtaaattg atatttggga tatgtggtgg ataccaaatg
    13801 ttaggtaaaa aattaaaaga cccttatcat gttgaaagtg aaattgaaga agttgatgga
    13861 ataggtcttt tagatacaga aacaacgttt gagaaagaaa agactacaac acaagtagaa
    13921 gctactattg ttgaatgtat cagtgaatac atgaataacc ttaagggaaa aaaagtaaaa
    13981 ggttatgaaa ttcatatggg aattacaaag cgaggtaatg atgtaagaag tttagattta
    14041 ataactaaaa aacttgataa agaagtgaat tatactgagg gaagtgtaaa taaagagggg
    14101 aatgtaattg ggacttatct tcatggaata tttgatgaaa taagctttac aagagcaatt
    14161 ttaaacaata taagaaagaa aaaaggttta ggagaattag aaagcaaagt aaactctttt
    14221 aatgaattta aggataaaga gtatgataag ttggctgatt ttttaagaga acatttagat
    14281 atagagaaaa tatatgagat tatgaaataa tgatgacctt tatttataat ttaggtagga
    14341 ggtgtattat gaaaaaaatt ttaattgcgg gtactaatag tggagtaggt aaaacaacaa
    14401 tatcattagg aataatgcaa gctttggtta agagaaatat gaaagtacag ccatataaag
    14461 tagggccaga ctatatagac ccttcatatc acacttttat aactggaaga cattctagaa
    14521 atttagattc ttatatgtta gatgatgaaa aaataaagta tatagtaaat aagtcctcaa
    14581 aagatgcaga tatttcagta atagaaggtg ttatgggctt atatgatggc tttggaatag
    14641 atttagataa ttgtacaagt tcacatactt caaaggtatt aaagtcacct gttatattag
    14701 ttataaatgg taaatctatg gcagcatcta gtgctgctat ggtacttgga tataaggaac
    14761 tagataaaga tgtaaatata aagggtgtta tagtaaataa tgtaaagact aatagccatt
    14821 atcaaataat taagagttct atagaaaaat attgtaatgt tgaagtattg gggtattttc
    14881 ctccaaataa caaattctct ttggaatctc gacatctagg attagttcct agtgtagaaa
    14941 tgaaatcact aagggaaaaa ttttatacct tagcagatga aattgaacac tatatagata
    15001 ttgacagaat aattgaaata tcagaaagtg atgaatttga gagtgatttt gattttcaaa
    15061 ctttgataga aaataataaa ataaaagaac atgttaataa taaatcaata gctatagcat
    15121 atgataaagc attcaatttt tattatagag aaaatataga gcttttagag gaacttggtt
    15181 tagaaataaa ctattttagc cctcttgaag atgtatcagt gccaaatgca gattatattt
    15241 atattggagg aggttttcca gaaatatttg gaaaagaatt agaagcaaat gagtcaatga
    15301 gaacttccat attagaagca cataacaata atatacctat ttatgcagaa tgtggaggac
    15361 tgatgtacct aggtgagaaa ctattgaatc aagaagaaca agaatttaat atggttggaa
    15421 tatttagtgg aattagtaag atgacatctt ctttaaagag atttgggtac tgtattggtg
    15481 tagccaagga aaatactctt ttatctaaaa aaggggaatc cattaaaggg catgagtttc
    15541 atcattcaat atttgaaagt gatgaaaaat gtgtatattc aatgagaaaa gaaagagatg
    15601 gaaatatagt agaagagtgg gatggtggtt atagtaaagg aaacactttg gctacatatc
    15661 ttcatactca tttttataat aacttaaatt gtatagaaaa ttttttgaaa aaaggaaatg
    15721 aataaatgag tattttatct atttatatag gatatgtgac agacttaatt gtaggagacc
    15781 catattcttt tcctcatcca gtgagattta ttggtaaact tataaatttt actcaaggta
    15841 taataagaaa ggtctctaaa aatgataggc agttaaaatt tgggggattt atattatggt
    15901 ttataaccgt tggaataaca tatttaacaa cttatgctat agtgaagtta tttagtttta
    15961 atatattatt gagtattgta gtgaatagtt ttataattta tacaacacta gccacaaaat
    16021 gtttaaaaga cgaagcatta aaaatttata atgtattaaa aactgaagat atagaaaaat
    16081 ctaggattca gctatcttat atagtaggta gggatactac taatttaagt gaacctgaaa
    16141 taataagagc aactgtagaa actgttgcag aaaatactgt agatggtata attgcgccta
    16201 tgttttatgc atttattgga ggagcaccac ttgctatggc ctataaagct atcaatacat
    16261 tggactcaac agttggatat aaaaataaaa agtataaaga tattggtttt gcatctgcta
    16321 agattgatga tatagccaat tatataccag ctagaatatc tgtcattctt atgacaattg
    16381 gaagtttctt tttaaattat aactatagga actgttttaa aatctctatt agagatagaa
    16441 agaatcataa aagtccaaat tgtgcttttt cagaaggtgc agtatctggt gctctaggaa
    16501 tacaactagg aggtaccaac gtttattttg gggaaaaagt atataagcca acaatagggg
    16561 ataagcttag ggagatagat aaagaagata taattaaaac taataaaatt atgtatgcat
    16621 cttcatttgt atctatgttg gtatttacac taatatatgt gttatgtagg atgattttaa
    16681 ttaacttaat tatataaatt attgtttgtt aacttaaggg ggacatagat gaaggattta
    16741 gggcatggtg ccaatgtaga tgaaatggca agattatatg ggaaaaaccc aaaagaaata
    16801 attgatttta gttcaaacat aaatccaaat gtattaccaa atttagagag atacatttta
    16861 aaaggattag aagagtgtag gaattatcca gatataaatt atacaaactt gagagaaaat
    16921 atatcaaaat atatagatat aaatccagac tttataatac ctggcaatgg tgcaacagaa
    16981 gtgatttatt tgcttatgaa gagtattaaa aaaaaattgg ctataataaa tccgactttt
    17041 tcagagtata gaagaagtgc tgagttaaat aatttagata ttatagattt agagttagat
    17101 ttagaaaata actttaaact taatatagat ataataaaag aaaatataaa aagatttgat
    17161 agtttattta tatgtaatcc taataatccg agtggtaatg tccaagattt aaaggaactt
    17221 gttcatttac tagataaaca taataaggta ctaataattg atgaaacttt catggaattt
    17281 gttgaagatg aaagtaaata tagtttggtt aaatatatag aatcaaataa aaatatattt
    17341 ataattaaag ctgtgactaa gttttttggt atgcctggtt taaggctagg atatggtctt
    17401 acaagcaaca ctgaaattat gaacaaaatt tatgaacaca aagagccatg gactatcaat
    17461 tcttttgcag atatactatc caattttatt tttgaagata aagagtatat taaaaatagt
    17521 aaagaatatt acatagaaga aagaaaatat atgttacaag agttgagaaa cataagaaat
    17581 ataaaggtct atgatacaga tgctaatttt atcttgataa gaatatataa aaaaacaact
    17641 aaagaattga aaaaagattt gtttaagcaa ggaaacatat tagttagaga tgcatctaat
    17701 tttataggat tagatgatag ctttataaga gtggctataa aatctcatga agataacaaa
    17761 attcttatag aaaatatgaa aaatttattg ggtgattaaa atgaaatctt atggaatatg
    17821 cccagcatct tgtggagaat ttgtacaagg tataattgat gatgaagaat atttatgttc
    17881 atatgcaata gatatgtatt ctaaagtgta tattgaagaa aaattagtag atataaattt
    17941 agggagatat aagtcaaggt tagcaattga aaaggtattt gaaaaattta atctacctaa
    18001 aaaatatact aaaaatattt ctttaaatat aaatagtaaa atacctgttg gaaaaggaat
    18061 ggcaagttca actgctgata taggagctac tataaaggca acattatcat tgatagataa
    18121 agatttaagt agcgaggaga tttctaaatt agcagcagaa atagaaccaa cagattctat
    18181 atttatagat aaaaatagta tatttaatcc tctaaatgga actgttataa aatacctagg
    18241 aaatctgacc aatgcgaaag ttgtaatact tgaaccaaac aaagtactag atactatgaa
    18301 aattagattg aggcaagatt ataataaatt aaaagtagag aataaagaag taataaaaaa
    18361 atcatttgct cttttagaag aaggattgaa aaaaaataat ttatctctag ttggagaggc
    18421 ttgtactttg agtagcttag caaatgagaa tatagaaaaa aaagagtatt taaatgaaat
    18481 aataaaaata tcaaaaaaat atggagcata tggagtaaat attgctcaca gtggaactgt
    18541 agtaggtatt ttaattgata agtttatgaa tgataaaaag atgatagatg ccttatgtga
    18601 atctaacata aattctgtct ataataaaat ttatacacaa aacataatta atggaggaat
    18661 taagggggaa atagaatgga atacataaag aatcctatga aaatagaaga aaaaagtttt
    18721 gaattgatac aagaaataat agacgaaatt agaccagatt ataaatttaa gaataatatc
    18781 gaagaaaaaa taataaaaag agctatacat actactgctg attttgatta cttagatata
    18841 ttaaaaatat cagaagaagc agttgatagt ataatagatg cattaaaaaa taatgcaagt
    18901 atttatactg atacaaatat ggctcttagt ggtataaata aaagaaagtt agaatcatta
    18961 ggatgtaagt ataaatgttt agtggcagat gatgaaactg taaaattagc taaagaaaaa
    19021 ggaattactc gttctatggc agcagtagaa attgcagcta aagaagaagg aagaaaaata
    19081 ttcgttttag gtaatgctcc aacagcttta tataaagtta tggagatgaa atcagaaggt
    19141 agattagatt tagatgcagt tataggagtt cctgtaggtt ttgtaggagc acaagaatca
    19201 aaagatgaag ttgaaaaaac agatatacca tatataattt ctaaaggtag aaaaggtggc
    19261 agtaatttag cagcagcaat agtaaatgct atattatata ctatgtaggt acctatatgg
    19321 aagaatatgt atatatagat ggaaaaaaat atagaagagg atatactaca ggttcatgtg
    19381 caacaggtgc atctaaggct gctgtatata tgttgattac aaaaaataga atcaatacta
    19441 taaatataga tactcctaag gggataccat tactactaaa agttgataat ataaatattt
    19501 ctgatacatt tgtggaatgt tccataaaaa aagatggtgg agatgatata gatgcaactc
    19561 ataccatgga tatttatgca agagctgaaa ttgtagctaa aaatgataag aacaaagggt
    19621 atcttactct aaaggatata gataatttaa gcaccaatag tgagtgtaaa agtgagctct
    19681 ataagtttat tagggtttat ggcggaactg gtattggtgt ggttacaaaa aaaggtctta
    19741 gcgtggatgt tggtaaacct gctataaatc cgactccact taaaatgata aatcatgaaa
    19801 taagaaagct tataggagat aattttgaat ctatacttgg aaatgataaa gttttaaaaa
    19861 taacaatctt tgctccacaa ggagaaactg tagcaaaaaa aacatttaat ccaagattag
    19921 gtatagttgg aggaatatct ataataggta caactgggat tgttgaacct atgagtgatg
    19981 agggttggaa gaagtctcta tctatagaac ttcagatgaa aaaagaacaa ggcttagata
    20041 aaataatatt ggttccagga aatcatggag aacagtttat aagagaaaaa ttaaacttag
    20101 atattaagta tgtagttagg gtaagtaatt ttataggcta tatgattaaa gaagctcaaa
    20161 gaataggata taagaaaatc ttaatggcag gtcatatagg taaatttata aaagtatctg
    20221 ctggtatttt taatactcat agtaaggtag cagatgctag aagtgagata ttagtggcaa
    20281 acttagcttt aatgggagct agatacgaat ttttaaataa aataaatcaa tgcgtaacta
    20341 ctgaagaagc tgtagagcta ataaataata gtgaatatag agaagtatat aatatattaa
    20401 gtaataaatg tagggagaga gtaaagcaat acttgaatga agattcagat gatattgatg
    20461 tggaagtaat catattttca atggataagt ctttattagg taaatcggat aatacagatg
    20521 atttagtgga ggtctttata tgataaacat aataggtcta ggacctggta acttagacta
    20581 tataacaaaa aaaggtgaaa atttaatttc cacttctgat gtattaattg gtggtaaaag
    20641 aaatttagag tctataaaaa attttgaagg agaaaaaata gttctggatt ctaatttaag
    20701 agaaattata gagtacataa ataacaataa agaaaagcaa atatcaataa tagcatctgg
    20761 ggacccacta atatatggga taggtagata tctatctaaa aatattgata ataagatgtt
    20821 aaacatggtt tctgggatta gttcattaca gtacatattt tcaaaaatat atgtagatat
    20881 gaatgatgta tatattacaa gtagtcatgg taaagtacca gattttgatt acatattatc
    20941 acataaaaaa gtatgtatgg ttacagattc aaagatagga ccaaaacaaa taagtagaga
    21001 aataatagat agaaatctaa ataaaattat tgttgtagga gaaaatctgt catatgacaa
    21061 tgaaaaaata acaatagcaa aaccagaaga gataataaga atggacaatt ttgatatgaa
    21121 tgttgtggtg attttagatg aggaatagtg aatttataac aggtaaagtt ccaattacta
    21181 aggaagaggt tagagcaata tcaataagta agttaaattt agtaaatgct aaaaacctca
    21241 tagacatagg ggctggtaca ggaagtgtaa gtgttgaagc tgcatataat tatcctaatt
    21301 taaaggttat atctatagag aaaaatgatg atgctataga gcttatagag aaaaataaaa
    21361 aaaaattcaa cttgaaaaat atagaagtga taaaaggata tgctccaatt aatctagggt
    21421 taaatgttaa agttgagtct atttttcttg ggggaacagg aaataatctg gaagaaataa
    21481 tagaatggtc taaaaaaaat ctcgttacag gaggaagact tgtagctaat tttataataa
    21541 tagatacatt taatcaaact ttaaaactgc ttaaaaagca tggatttaaa gaaatagatg
    21601 tatgtgtttt gaatgtttct aaactagaga aactaggaaa aggtgaatat tttaaaccac
    21661 taaatcctat atatataata tcttgtgaaa aaggggaaaa tgatgatgaa taaagtacat
    21721 tttgtaggag caggacctgg agataaagag ctgataacat taaaaggata caaattatta
    21781 agtaatgcag atgtagtgat atatgcaggt tcacttgtta atcctgaact tttggaatac
    21841 tgtaaagaag attgccaaat acataatagt gcacatatgg atttacaaga gataatagat
    21901 gttatgagag aaggtataga aaataataaa tctgttgtaa gactgcaaac aggtgatttt
    21961 tctatatatg gttcaattag agaacaagtg gaagatttaa ataaattgaa tatagattat
    22021 gactgtactc ctggagtaag ttctttttta ggggcagcat catctttagg ggttgaatat
    22081 acagttccag aaatttctca aagtgtaatt ataacaagaa tggaaggcag aactccggtt
    22141 ccagagaaag aatcaataca atcatatgct aagcatcaga catctatggt aatattttta
    22201 tctgttcaag aaattgagaa agttgtgtct aaattactgg aaggtggata tccaaaagat
    22261 actcctatag cagttatata caaagcaact tgggcagatg aaaaaatagt taaaggaact
    22321 ttaagtgata tagctgtaaa agttaaagaa aacaacataa ataagactgc tttaataatg
    22381 gttgggagat ttttaggaga agagtataat aattcaaagt tatatgataa ggatttcaaa
    22441 catgaatata gagggtaata ttgctataat ctgtattaca gaaaatggaa aaaaattagc
    22501 tctaaagata aattctctag taaataactg tgttatatat caagtaaaaa ataaaaaaag
    22561 taatttagtt gttgaagaaa catgtgtgca tacagttcaa aagaagttaa gtgattttgt
    22621 aggagaaata tttagcaaat ttgattacat agtatttata atggctacag gtatagttgt
    22681 acgaagtata gcaccataca taataagtaa gtttagtgac cctgcaattt tggtaatgga
    22741 tgaaaaaggc aaaaacgtaa taagtttatt gagtggtcat attggagggg caaacagctt
    22801 gacacagtac ataagtagtc ttattgatgc taatccagta ataacaacag ctactgatat
    22861 aaatcaaaaa gcttccttag atatgatagc aaaaaaacta aatgcacata ttgataattt
    22921 tagagaaagt gtaaaagaag taaattctat gctagtgaat aataaatgtg ttggattata
    22981 tgttgatgaa ggatatcaag ttgatactag gggatttaaa cttatagaaa gtaaggatga
    23041 gttaagagat agtttttcaa acacatatat agaaaaatta gttgtagtaa gcaataaaat
    23101 aaattgtatt gaagatattg agaatttaaa cctagataat aaatttgaac attgctatag
    23161 tacccaaaaa ataataaagg taattccaag agatatagtg ataggaattg gctgtagaag
    23221 aaacacagat agtgaactga tgtataattc acttgaagag tttcttaata aacaaaatat
    23281 agatattaga gcaataaaca cagttggaag tatagaactg aaaaaagacg aaaaagcaat
    23341 aattgaatta tcaaaaatat tgaaggtgcc atttaaaaca ttttcagtag atgaaatatc
    23401 taaggttgat aatctttttg ataaatctga ttttgttaaa aaaaatgtgg gtgtgtattc
    23461 agtagcagaa cctgtagcat atcttttgag caaaggtaat ttgttagttg aaaaacataa
    23521 atataaagga ataacttttt cagtggggag aataaaaata tgatatacgt tgtaggaatt
    23581 ggaccaggaa gtaaagatac aatgacttta gaagcaataa aagcaataga agattctgag
    23641 gctattgttg gatataagac ttatataaaa ctaatagaag aatttataca aggtaaagaa
    23701 ataatccaaa atggtatgag gcaagaagtt gatagatgta aacaagcagt ggaggaagca
    23761 aaaaaaggaa aaaaagtagc agttgtaagc agtggtgatg ctggaatata tgggatggca
    23821 ggtcttattt tagaattaat atctaaagaa gatgaagata taaaagtaaa ggtaattcca
    23881 ggcgttactg caagtattgg tgcagcagct atacttggag ctcctattat gcatgatttc
    23941 tgtcatataa gtttgagtga tttaatgact ccgtgggaag taattgaaaa aagattgaga
    24001 ttggctgctg aagccgattt tgtaatttgt ttgtataatc caagaagtaa gggaagaagt
    24061 gaacatctaa gcaaagcatt caaaataatg ggtgagttta aaggtggaga tacacctgtt
    24121 ggagtggtaa aagatgtagg tagagaaaaa gaagaaaagt ttgtatgtac ttttgaaaat
    24181 atggactttg aaaaagtcga tatgactaca atggttataa taggaaataa gtctacctat
    24241 attaatggtg aattgatgat aactccaaga ggttatacag tatgatttta attttaggag
    24301 gaacttcaga tagtttatct atatgtgata agataaatga actgaaaaat caaccatata
    24361 tcctttcagt tactacagat tatggaaggg aacttgcaca acaacatgca gagaatgtaa
    24421 ttttaggtaa gcttggaaaa gaagatatgc taaaatttat aaaagataat aatatagtaa
    24481 aaataataga tgccactcat ccttatgcag ttgaagtatc caaaactgct attacttgtg
    24541 caaaactatt gaatatagat tatataaggt ttgagagaaa atctttaatt gaagaaatag
    24601 attatgataa taaatttata gtagatacaa ttgaagaagc ctgtgaaata gcaaataaaa
    24661 tagggacaaa tatattcata ggaacaggaa gtaaaaattt gaacagattt atagaggaaa
    24721 taagtgataa aaatttagtt gttagagttc ttccaacgag tgaagtcata ttaagttgtg
    24781 aaaaattagg tttaagtgca gataatatag tagctatgaa aggtccattt agccagtata
    24841 taaatgaaga aacatataaa cattacaata tagatttagt cataacaaaa gaaagtggaa
    24901 tagctggtgg gttcttagag aaggtaaatg catgtaaaac tttcaatata ccagtaataa
    24961 ttataaaaag agaatcgatt aactacgaaa atgtatttaa tgacataaat aaaattaaag
    25021 acatacttgt gtaattttta ggaggagaat atatgaagaa agccttatta gtagtgagtt
    25081 ttggtacaag ctatcatgaa acgagagaaa agacaataga tatatgtgaa gataaaataa
    25141 aaaattcgtt aaaaagccat gattttttta gagcttatac atcaaatatg ataataaata
    25201 aaataaaaaa aagggatgga atagaaatag ataatccaat acaggcttta gataaaatat
    25261 atgaacaagg atatgatgaa gtaataatcc aaacacttca tattatatgt ggcgaagaat
    25321 ttaacaaatt aaaagaacaa gtagatggat attcttctaa attcaaaaag attgtattag
    25381 gaagaccttt gcttacacat attgaagatt atcaggaagc tgtagaggca attaagcatc
    25441 aaattcctca tatggaaagt aatgaagcag tagtatttat gggacatgga acacttcatg
    25501 aatcacattc agcgtatcca gcattggaat acatgcttag ggataatgga ataaatgctt
    25561 atgttggtac agttgaaggg tatccagaaa tagaacatgt aataagaaga ttaaaagaag
    25621 gaaatataaa aactgtaaat ttgatgccat ttatgttagt tgcaggagac catgctataa
    25681 atgatatggc aggagatgaa gaagattctt ggaaaacaat attagaagaa aatggattta
    25741 acgtaagagt tcatttaaaa ggactaggag aaaattctta tatacaagat aaatttgttc
    25801 gtcatgctgt aaaatgtgca gagaatgcga agttctatgg tataggagca gggccaggag
    25861 atggttcttt acttacgata aaagcggtta atactttaaa gaaactggat attctataca
    25921 ctccagaatc aaaaaagggt ggagatagtc tagctttatc aatagttagt gaatatttac
    25981 ctgaatcagt ggaaataaag tctaggcatt tcccaatgag ttttgatggg aatgaaaaaa
    26041 gtttagcttg ggacaatgtg gcacaagaaa ttatatcaga tgttaatgat ggtaaaaatg
    26101 taggatttgt tacattagga gaccctatga tttatagtac atatgtatat ataatgaaaa
    26161 gaatattagg agaggtagaa gtcgaaacta taccaggaat ttcatcattt tccaatatag
    26221 catctaatca gaattttcca ttagtcatgg ataaagaagc tttagtggta attccttgta
    26281 ctatggaaga tgataagata gagtatgctt tgcaaaatta taattcaata gtattgatga
    26341 aagtatataa aaattttaaa gaaataataa aaaaattaga aaagtatgat ttaatagaac
    26401 atgctatttt agtaagtaat tcttcccaag aaagcgaaaa tgtgtttaca gatttaaaag
    26461 aagcgcacct tgaagataag atatcttatt tttctactat actagtaaat aaagataata
    26521 aaataaaata ggtgaaagca atatttaaag gagacttaat atgaatatag tggttggaac
    26581 taggggtagt aatctagcgc ttatacaaac agaatgggta ataaatgaat taaaaaagaa
    26641 gtatccagaa attagttttg agattaaaat cataaagaca aaaggagatt taattcaaaa
    26701 tgtttcactg gataaaatag gtgataaagg attgtttgtc aaagaaatag agcaacaatt
    26761 attagatgga aaaatagaca tagcagtaca tagtatgaaa gatatgcctt cttatttagc
    26821 agatggacta aaatttgctc atacgcctaa gagagaagac ccaagagatg ttctaatact
    26881 tagagaagga tataaaaatt tagatgattt gcctcatgga gcagtaatag gaacaggaag
    26941 taaaagaagg aagtttcaat tattgaaaca aagacctgat ttaaatatag tacaagttag
    27001 aggtaatgtg gaaactagga tacgaaaaat aaaagatgaa aacatgcatg gtattgtgtt
    27061 ggcagcatca ggaataatta gagctaattt acaagataaa ataagttctt atttacctgt
    27121 tgatgttgtt attccagcac ctgcccaagg agctttagct atagaaatta gaagcaatga
    27181 tagtacgata gagggaatag taaattcact gaaggatgag aatacagaaa tccaaatatt
    27241 ggctgaaaga ggttttttag atggtgtaaa tggaagttgt catattccaa tggcagctta
    27301 ttgtgaaata atccaagata aaatacatct tactggctta tatggagatt cagaaggaaa
    27361 aaaagtagta atcaagtcta tagatggaga tatttcttct cctagagagt taggtttaaa
    27421 gttagctaaa cttgtattga aggagtatga gaattatgaa gggtaaggtt tacttagtag
    27481 gggcaggacc aggtgattat aaactcatga ctttaaaagg tttagagtgt ataagaaaat
    27541 cggatgtaat agtatatgat agattagcaa atagtagtta tttaagagaa gctaagcctg
    27601 attgtgaact tatatatgta ggcaaggcat caagtaatca tattttgcca caagaagata
    27661 taaatagaat tatagcagaa aaagctaaag aaggaaaaat tgtaactcgt cttaaaggag
    27721 gagaccctta tgtatttggc agagggggag aagaagcaga gactttagta gatgaaggta
    27781 tagaatttga agtagttcca ggtataactt ctgctatagg aggtttatgt tatgcgggta
    27841 ttcctataac acatagagac catgcttcgt catttcatgt aataacaggt catttgaagg
    27901 gtgatgatag tggagaatta aactggaatg ctcttgcaaa taacaaaggt actcttgtgt
    27961 ttttgatggg aatttcaaac ctagagaaaa taagtgaaaa cttaatgaaa gaaggaaaag
    28021 ataaggatac acctgttgct cttataagtt gggcaactag atataatcaa agagttgtta
    28081 catctacact tgaaaatgtt tatgaaactg ctattaaaga agaagtaaaa ccgccaactc
    28141 ttatagtagt tgggagtgtt gtaggactta gagaaaagtt gaacttcttt gaaagtaagc
    28201 ctttatttgg aaaaagtatt gctgtaacta ggtctagaaa tcaaaatagt gtattggtag
    28261 aaaaaataat ggatttaggt ggaaatccta tagaaatacc tactattaag gtagaaaaaa
    28321 tacaaaataa tattaactta gaaaatgaaa taaaaaatat aaataagtat aattatctaa
    28381 tactaactag taaaaatgcc gtagaaatct tctttgaaaa gatatatgaa atgaattttg
    28441 atttaaggat attatctaat ttaaaggtat gtgctatagg ttctgctaca tcaaatgagt
    28501 tgaaaaaaag aggaattata gcggatatag taccaaaaaa atttgtagca gagtcactgt
    28561 atgaagagtt agcgcctatt ttaaatgatg acgataatat acttatacct agagcagaaa
    28621 atgctagaga ttatctggtt gacaaactca aggctatttg tgaagtaaca gaagtacata
    28681 tatatagaac agtaatagat gaggagaaaa aaggtgagat attagatatt ttaaattcta
    28741 atgacataga ttatataaca tttactagtt cctcaacagt aaataatctt gttgaaatta
    28801 taggaaaaga aaatattaaa atattagaaa aaataaaaac actatctata ggtcctataa
    28861 ctacagagac aataaatagt ttgggaataa aattgtacaa agaatctgct acatctacta
    28921 tagattcgtt agtagattta attataaaaa attaattttg gaggcttgaa tatgttaaga
    28981 agaccaagaa gactaagagc aagtaaagca atcagaaatt tggtaagaga aactaaggtg
    29041 aatgtagaag acttaatata tccactgttt gtagtagagg gggaaaatat aaaagaagaa
    29101 attaaatctt taccagatgt ctatcattta tcaatagata tgctagaaga cgaaataaaa
    29161 gaaataagtg agttgggaat tgaacatata atgttatttg gtgttccaga tgaaaaagat
    29221 gagtgtgctt cacaagcttt taatgataat ggaatagttc aaaaggcaat tagaaaggta
    29281 aaagaaataa attcagatat aaatgtaata acagatgtct gtatgtgcca atatacaagt
    29341 catggacatt gtgggatttt aactgatact ggttatgttg ataatgataa atcattggag
    29401 tatttggcta aaatatctgt aagccatgct aaagcaggag ctgatatggt tgctccatct
    29461 gatatgatgg atggaagaat acaagcaata agagaagctt tagatgaaaa tggattagaa
    29521 aatgttggaa tcatggctta tagtgttaag tatgcttcaa attattatgg accttttaga
    29581 gaggcagctc attcagcacc atcttttgga gatagaaaaa catatcaaat ggacccagca
    29641 aatagcaatg aagctataat agagactgaa cttgatatat tggaaggtgc agatatactt
    29701 atggttaaac ctgcattatc atatttagat attataagaa gagttaaaga taattttgac
    29761 ttacctctag cagcttataa tgtaagtgga gaatactcta tgcttaagtt tgctgtgaag
    29821 gaaggtttat taaatgaaga tgtaattctt gaatcaatta catctataaa aagagcagga
    29881 gcagatataa taataactta ttttgctaaa gacattgcta aactgttgaa aaaataaatt
    29941 ctagcagtat aaagaatatc aaaaagtata ggaattgtaa ttaactatta tacagtagtt
    30001 ttgtttagac atttattgaa gaataataga aactaatttg caatttctat actttattta
    30061 tgtaactaaa taaaatttgg aggtagaaga tgctatatcc tattaattta aaattagatg
    30121 agttagatgt agttataatt ggtggtggag aagttgcata taggaaatgt aaaaattttt
    30181 tagagtttaa caaaaatgtt actatagttt ctaaacaaat tttaaataaa ttttatgatt
    30241 taaaaggaaa tataaaaata ataaaagatg attataaaga aatatatata aaagatgctt
    30301 ctgtaataat tgctgccaca aataatagag agttaaatat ggaaatagga ttatattgta
    30361 aagcaaaaaa taagctagtg aatgtagttg ataatataga aatatcgaac tttactgttc
    30421 catcatatat aaaaagagga gatttactta taagtgtgtc tactgggggc aagagtcctt
    30481 cattatcatc aaagataaaa aaagaaattg aagagagata tactgaagat tatgaggagt
    30541 atttaaatgt attaggagat ataagaaaaa aggttataaa aaaatacgaa gataaaagta
    30601 aaagaaaaga tgtgcttaat atgttggtaa cacttgacct agaagaactt aagaagtttg
    30661 atatataaaa aataaaataa aaaatgttaa aaaggtgttg acggat
//
LOCUS       DAMJZE010000028        29958 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJZE010000028 DAMJZE010000000
VERSION     DAMJZE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.028"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            169..309
                     /locus_tag="PC541_001180"
     CDS             169..309
                     /locus_tag="PC541_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838651.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(484..804)
                     /locus_tag="PC541_001181"
     CDS             complement(484..804)
                     /locus_tag="PC541_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2838652.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(924..1661)
                     /locus_tag="PC541_001182"
     CDS             complement(924..1661)
                     /locus_tag="PC541_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2838653.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1876..3699
                     /gene="acd"
                     /locus_tag="PC541_001183"
     CDS             1876..3699
                     /gene="acd"
                     /locus_tag="PC541_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2838654.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3914..8212
                     /locus_tag="PC541_001184"
     CDS             3914..8212
                     /locus_tag="PC541_001184"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2838655.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8416..8877
                     /locus_tag="PC541_001185"
     CDS             8416..8877
                     /locus_tag="PC541_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2838656.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8907..10061
                     /gene="nusA"
                     /locus_tag="PC541_001186"
     CDS             8907..10061
                     /gene="nusA"
                     /locus_tag="PC541_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2838657.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10068..10349
                     /locus_tag="PC541_001187"
     CDS             10068..10349
                     /locus_tag="PC541_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2838658.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10339..10650
                     /locus_tag="PC541_001188"
     CDS             10339..10650
                     /locus_tag="PC541_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2838659.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10672..12612
                     /gene="infB"
                     /locus_tag="PC541_001189"
     CDS             10672..12612
                     /gene="infB"
                     /locus_tag="PC541_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2838660.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12655..13035
                     /gene="rbfA"
                     /locus_tag="PC541_001190"
     CDS             12655..13035
                     /gene="rbfA"
                     /locus_tag="PC541_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2838661.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13016..13999
                     /locus_tag="PC541_001191"
     CDS             13016..13999
                     /locus_tag="PC541_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF2838662.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14012..14917
                     /gene="truB"
                     /locus_tag="PC541_001192"
     CDS             14012..14917
                     /gene="truB"
                     /locus_tag="PC541_001192"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2838663.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15048..15977
                     /locus_tag="PC541_001193"
     CDS             15048..15977
                     /locus_tag="PC541_001193"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2838664.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16081..16338
                     /gene="rpsO"
                     /locus_tag="PC541_001194"
     CDS             16081..16338
                     /gene="rpsO"
                     /locus_tag="PC541_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2838665.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16578..17423
                     /locus_tag="PC541_001195"
     CDS             16578..17423
                     /locus_tag="PC541_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2838666.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17548..19659
                     /gene="pnp"
                     /locus_tag="PC541_001196"
     CDS             17548..19659
                     /gene="pnp"
                     /locus_tag="PC541_001196"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2838667.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19925..20668
                     /locus_tag="PC541_001197"
     CDS             19925..20668
                     /locus_tag="PC541_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2838668.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            20826..22073
                     /locus_tag="PC541_001198"
     CDS             20826..22073
                     /locus_tag="PC541_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2838669.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22108..22353
                     /locus_tag="PC541_001199"
     CDS             22108..22353
                     /locus_tag="PC541_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2838670.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22363..23586
                     /gene="dapG"
                     /locus_tag="PC541_001200"
     CDS             22363..23586
                     /gene="dapG"
                     /locus_tag="PC541_001200"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2838671.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23699..24433
                     /locus_tag="PC541_001201"
     CDS             23699..24433
                     /locus_tag="PC541_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2838672.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24556..26967
                     /locus_tag="PC541_001202"
     CDS             24556..26967
                     /locus_tag="PC541_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2838673.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26970..28028
                     /gene="mnmH"
                     /locus_tag="PC541_001203"
     CDS             26970..28028
                     /gene="mnmH"
                     /locus_tag="PC541_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2838674.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28022..29356
                     /gene="rimO"
                     /locus_tag="PC541_001204"
     CDS             28022..29356
                     /gene="rimO"
                     /locus_tag="PC541_001204"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2838675.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29343..29885
                     /gene="pgsA"
                     /locus_tag="PC541_001205"
     CDS             29343..29885
                     /gene="pgsA"
                     /locus_tag="PC541_001205"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2838676.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
BASE COUNT        12087 a         3189 c         5693 g         8989 t
ORIGIN      
        1 tatatgaatt ttaataggta aaatatccta ttgttttgta gatatatcct aattatgtca
       61 aaaaataaaa aaatttttat ttattagagt ttttggaaat aataataaca gatattataa
      121 ttaattaaca gtttttgtaa gtatttgaca atagagaggg tgtaacaaat gggaataata
      181 gagtcagcat caaaattggc tgagatggtt catttattgg ctgttgaaaa ggggataact
      241 gatatagaag catgggatga agctgtgaaa gaatacagca aaatttatga agaaagaaga
      301 aatgaataga atgataaaaa atgaaaactt gatgataaaa aataacttta tttaaagaaa
      361 ttcaattttt atagaagtat taaaaataat ataaataaaa agatggtatt tcaaatgtaa
      421 gtaaaaaatc attttgggat accatcctta ttatttattt taataatata taagtaataa
      481 atcttactta tccatatttt ttatgtaacc tcttttttct aactttttta tatgctcctt
      541 acgtatttca tcaagattaa taccatattg ttcttctagc ttaaacatca tagtaacaca
      601 tgtctgagct acatccatta gttccttagc agttttcttt ataatatcaa cttcatctaa
      661 atcattattt tctccactta gatttctgaa tttacctata cattcagcta attcaccttg
      721 ttcttcaccc attttgataa atgtagattc tagtgttggg tctaaattat taagctctgg
      781 taaagatata gtttttagtt tcataaaagc tcctttcaag ttattcgtat ttatactaaa
      841 acatcataac aaaaataata ctaaaaaaaa actttaaatt caacataaaa acttacatat
      901 ataataaaaa tcgtgaaata aatctatatt tgtcttaata cagcctcata taaaacttct
      961 ccaataggtt tatttattac aagagaagct gacttatcat agactgtatt tgctttatta
     1021 ataagagcaa tataatcacc tttgtaataa tctataaaac ttgctgctgg gtacacagct
     1081 aatgatgtac ctcctataat caataaatca gcattactta tagcagaaat tgttttagta
     1141 atgacatcac tgtcaagagc ttcctcataa agtactacat ctggttttac tatagaacca
     1201 cagttgtcac agtaaggaat attacctccc agatttaaca tagattctaa gtcaaaaaac
     1261 ttaccacatt tagtacaata atttctatgt acagaaccat gaagttctag cacattttta
     1321 cttccagcca tttgatgaag tccatcaata ttttgagtta tgacagcttt aagcttaccc
     1381 atttcttcga gttttgctaa agctatatga gcattattgg gttttgcatt aggatatatt
     1441 aatttatcct tataaaattc aaaaaattct tctggatatc taacaaaaaa agtatgtgaa
     1501 actaattgtt cagcagtaaa ttgtttattg agtttttggc taaataatcc agtagaactt
     1561 ctaaagtctg gaatattaga ttcagttgaa acacctgctc caccaaaaaa tacaatatta
     1621 ttgtgattag ctattaagtc ttttaagcta tttgcatcca tagttacacc tccaaattat
     1681 tatatataaa ttgtactaca tattaggtcg tttatgtttt attaatttaa ataaatatat
     1741 agtttaataa gttaaataaa gggaatataa ataaaatgta tacagagtat aaatagacaa
     1801 tatttactaa aataacaagt tgtcgtataa ttatattaaa attgttttag tgtataagaa
     1861 gggcggggga agtacgtgaa gaaaaaagca gctttagcaa cattggctat gttaccctta
     1921 ggtgtggtaa atgcacatgc tgatggagat ataggtatag tgactataaa ttatttaaat
     1981 gtaagaaatg aaccaactgc cgaaagtagc atagcctttg ttgctaaaaa agatgataaa
     2041 gttttgatta aagactcttc taatggatgg tataaaataa aagctgaatc tggacaagaa
     2101 ggttgggctt catcaaaata tatagcaaaa tcaaatagtg actctttaag aacgtctaca
     2161 aataaagaaa aacaagttat ttcaaatagt ttaaatatga gaaatggagc aggtacaagt
     2221 tacagagtaa taactgtact aaaaaaaggt caaaaagtag aagtaatatc agagagtaat
     2281 ggatggtcta aaatcaaata tgatggaaga ctaggatatg tatctagctc ttatttagga
     2341 gacgtttcaa actcaactaa taaatcaaaa actaaacaag tgaatacaac ttcactaaat
     2401 gtaaggagtg gaccaaatac aagttatggt ttgttaggca agttgccaaa aggaagtaaa
     2461 gtagaagtaa tatcagaagg taatggatgg tccaaaataa agtataatgg aaaagatgcc
     2521 tatgtatcta gtatgtactt atcggatgta agtcaaagta attcagacaa ttctagtcaa
     2581 agcaatgata aaaagaatac tgataaggtc gtaaatacag cttctttaaa tgtaagaagt
     2641 ggaccaggtt ctacatatag taagttggga aaagtttata aaggaagtaa agtaactgta
     2701 ctatcagaaa gtagtggatg ggctaagatt aattttaaca ataaagaagc atttgtagta
     2761 ggcaattatt tatctacttc agcagatact tcaaataata actcaaatag taactctgat
     2821 aacagttcta atagtaatgg caataattct tcatcatcag gtcaagtaaa tggtatgtca
     2881 ggtataagtg gggctaaaat tgattataaa tctttgagtt atactttaga atctcatata
     2941 agtaaacaag ttgagaaagc agcatcagga ggaaatgtga tagctccaag taataggaaa
     3001 agcactccaa gtcctgaatt tagtactttt tcagctcaaa gaacaagctc atttgtaaat
     3061 gcaagttcaa gtgatataga atattattta aatcctaaaa attttacaaa tactactaaa
     3121 ggtatgatgc agtttttaaa gattaacagt tatagagatg gtatttcaga atcaagtctt
     3181 aactcgtatc ttaatggttt atcttctagt gtatttaaaa accagggagc agcctttata
     3241 aatgctgcca agaagtataa tatagatgtt gtatatctag tatctcatgc tatgtgggaa
     3301 actgcttatg gtaagtctac acttgcccaa ggtcaaacat taacatctta taagggacaa
     3361 cctcttagta agccagttaa agtatacaat ttttttggta taggagcaat agataaaagt
     3421 gctaatgttt caggtgcaga agcagcgtat tccaatggct ggactagtgt agaagctaca
     3481 atagatggct ctgcaaagtg gatatcacaa aattacgtaa atagttctaa gtacaatcag
     3541 aatactatct acaagatgaa gtggaattat gattatacat ggcatcagta tgcaactgat
     3601 gtgaactggg ctaatggaat ttctggaatt atggaaaatt taattggtct ttatggagga
     3661 ggaagtagtt tggtttttga agttcctcaa tacaagtaat acataaataa aaaatataga
     3721 ctatgggtag ttttcatttt atttaaatta tctatagtct ttttgtatat aatttataat
     3781 ctgtaaattt aatgctaaca aaacttttat gttataatat taattctaag taaatataag
     3841 tgagtaatag gtagaaatag ataaaaagtg gtaataatat atataaagat aattttgaga
     3901 aaggaggata tatatggaaa gtattaagga atatttggat aagttagaaa ttaataatag
     3961 tggtcttgga aagcaactta aagaagttta tataaataga gtagtttatt ttaaggaaga
     4021 taagatagtt tatttttatc taacttctaa agatattgtt tctcatgaac ttttagataa
     4081 atttaaagaa gaattgatgt ataaactcga ttattttaaa gatatgagga tgaagataag
     4141 atttactggt cttgaaagaa aatccaataa agatgttata aaaaaatatt ggaataatat
     4201 attgtatata ttaaagtatc tttgtccatc tattgcaggg tggtataaac aagtagaatt
     4261 tctttgttta gaagaagaat taaaaataaa acttccaaaa ggtatatttt atgagcgatt
     4321 aatgaaaaaa aatgtaactt atgtacttaa aaccgtgctt agcgaagagt tgggattaga
     4381 tttaaatata acaatagaaa aagctgttga tgaaaaggta aataaagaaa gactcataag
     4441 aatcaatgat agagaaatgg aagaaaaaat tagggcttta gaaattggaa aagttaataa
     4501 ctgtgaagaa aatgaagagg aaagttatgt cattaagtct gatgtagatg aaaatttaat
     4561 ctatggagat aatgcaaatg ctatgattga aaatatagtt gaattaaatg catcttctgg
     4621 aacagttgca gttgttggag atatatttga tgttgacact aaagaactta aaaacggaaa
     4681 aatacttatg atagcttcaa ttacagatta tactagctca ataagctgta aacttttttt
     4741 aacagataca aataaggatg gagtacttga aaatgttaaa aaaggagcat acttaaaaat
     4801 caagggtgac atagtttatg atacatatca aagagaaatt agtatgatga ttagtgggat
     4861 aagaaaagaa actagaatag agagagaaga taaatcagag gaaaaaagag tagaactcca
     4921 tgcacataca caaatgtctt ctatggatgc tatttgttct gttaaaaagc ttgtagagcg
     4981 tgcagccaaa tggggtcatt ctgctattgc tataacagac catggtgttg tacaaggatt
     5041 tccagatgct atgaatgcag gtaagtcaaa taatataaaa atactttatg gagtagaagg
     5101 gtacttggtt gaagatgatt ctctcattat agaggatgca aatgataaag aactttcgca
     5161 gacatttgtt gtatttgaca tagagacaac tggtttttct aatacaaatg ataaaataac
     5221 tgagattggt gctgtaaaaa tagaaaactt taagattgta gatagattta gtgagttaat
     5281 aaatcctgaa aaagatattt cttataagat acaggaattg acaggaataa ctaatgaatt
     5341 aattaaagac aagccaacca ttgaagaagt tcttcctaag tttatggaat ttgttggaga
     5401 tagtgtatta gtagcacata atgcagagtt tgatactgga tttatatcgc aaaaatgtag
     5461 ggaacaaggc ttgttatata ataacaaaaa agtagataca cttatgttag caagagtaat
     5521 gttgccaaac ttaaaaagac atagattaaa tgtcgttgca aaagaacttg gtattcctct
     5581 tttaaatcac catagagcag tggatgatgc tgaagctaca gcacttatat ttaataagtt
     5641 tttgcagatg cttacagaaa aaggtgcaaa aactctaagt gatgtaaaca atatacttgg
     5701 aaagattgat tacacaaagc ttagcacaaa tcatataacc ttgatagcaa aaaattctat
     5761 aggtataaaa aatttatata aaataatttc agatgcacat gtaaatcatt tttttagagc
     5821 accaagaatc cttagaagtg tattaaatga atataaagaa ggattaataa ttggttcggc
     5881 ttgtgaagct ggggtggttt ttcaagctgt aaagaaaaac gtgtctgatg aggaaatgaa
     5941 aaaaataata gatttatacg attatattga agtaatgcct atagataata atagatttat
     6001 gattgataaa ggagaagtaa aggacgaaga agagttaagg gagttgaata ggaaattaat
     6061 tgatacagcc aaaaaatttg acaaaattcc agtagctact ggggatgttc attttttaga
     6121 caaacatgaa gctgtcttaa gaaaagttct taaatattct caaggtttta aagtagatga
     6181 agaagaaact tatttacatt ttagaacaac agatgaaatg ttggaagagt ttagttatct
     6241 tggagaagaa ttggcatatg aagttgtagt tgaaaatagt aatctgattg ccgatatggt
     6301 tgaagatata aaacctatac cagatgatac ttatcctcca attatagagg gctcagatgt
     6361 ggagttaaga gaaatgtgtt acaaaaaagc caaacgaatc tatggagacc caataccaga
     6421 aatagttcaa aaaagactgg atagagaatt aaattcaata ataagcaatg ggtatgcagt
     6481 tatgtatatt atagctcaaa aactggttgc aaaatctctt tcagatggat atttagttgg
     6541 ttcaagaggc tcagttggct catcttttgc agctactatg agtgatataa cagaagttaa
     6601 tcctcttcca gcacattata tttgtgaaaa cgaagactgt aaatattcat tcttttatga
     6661 aataggtgaa tggggctcag gtgttgattt accagacaag gattgtccaa agtgtggaag
     6721 aaaacttgct aaaaacggtc atgatattcc atttgaggtt ttcttaggtt ttgaaggtga
     6781 taaagaacca gatatagact taaatttctc aggaaattat cagccaacaa ttcataaata
     6841 cacagaagaa ctatttggag aggggtatgt atatagggct gggactatag gtactgtagc
     6901 agaaaaaaca gcttttggat acgcaagaaa atatgtggaa gaaaataata taagtgtacc
     6961 aaatgcagaa gtacttaggc tttcaaatgg atgtactgga gtaaaaagaa cctcaggaca
     7021 acatcctggt ggagttatgg ttataccaga ctataaagat gtatacgatt ttacacctat
     7081 acagtatcca gcaaatgata cttcttgtgg tgttataact actcactttg attatcattc
     7141 aataagtggt agaatactta aacttgatat acttggacac gacggtccta ccattataag
     7201 gatgcttgaa gatatgactg gaataaatat aacagaaatt cctttagatg ataaagagac
     7261 tatgtctcta tttacatcta cagaagcatt aggtgttaca ccagaagaaa taaactgtcc
     7321 tataggttgc cttgctgtac cagagtttgg aactaagttt gtacgacaaa tgttacttga
     7381 cacaaaacct actacatttg cagaacttgt acgtatatct ggtctctctc atgggacaga
     7441 tgtttgggta aataatgcac aagacttagt tagagaagat atagttggat taaaagatgt
     7501 tatatctaca cgagatgaca taatgaacta tttaatattt aaaggattac ctcccaaaat
     7561 gagctttaca ataatggaga gtgtaagaaa aggtaaaggt ttaaaaccag aacacattga
     7621 agaaatgaaa aaaaataatg ttccagaatg gtatataggc tcatgtaaaa agattaagta
     7681 catgttccca aaagcacatg ctgttgctta tgtaatgaca tcatttagaa ttgcatattg
     7741 taaagttcac tatccagaag cattttatgc gacttatttt acgacaaaag ttgaggattt
     7801 tgatgcagat ttaattgtta aaggattaga tgctataaaa tcaaaaataa atgaaattga
     7861 gtcactggga aatgatgcaa ccacaaaaga aaaaggtatg ttgaccgttt tagaagttgc
     7921 tcttgaaatg tatgctagag gaataaaatt attaccagtt gatatatata aatcagatgc
     7981 aactgagttt atagttgtag gagaaaaaac attacttcca ccaatggcag caatacaagg
     8041 tcttggagga aatgcggcta taaacatcca aaatgaaaga aaaaatggtg aatttatatc
     8101 aaaagaagat ttgagaaaga gaacaaaaat atcaaaaacg gtaatagaaa ctcttactaa
     8161 tcatggttca cttgagaata tgagtgagag aaatcaattg tcattgtttt aagttagccc
     8221 aaaaagttgc aaataaaaga attttatgat atcatagtat agaaaatagt atactttgca
     8281 gaaactaaga gtgagacagc tgttctcact cttttatttt aaatatatta ttaaaaagct
     8341 ggtttatttt tattaaatat attttattta agattatata aactggtcat ttacaattga
     8401 ataggaggaa ctttaatgaa aaaaaattta gaagctacaa ttgaggagat tgtaactaaa
     8461 ataacggatg aacatggatt tgaaatggtt gatgttgaat atgtaaaaga agcaggagaa
     8521 tactacttaa gagtatacat agataaagaa gaaggaatat ctttaaatga atgtgaatta
     8581 gttagtagag aattaagtcc aatactagat gaaaaagacc ctataaaaga aaattacttt
     8641 ttagaggtat cttctccagg gcttgataga gcattaaaaa aagacagaga ttttgttaga
     8701 tatcaaggta gagatgtaga tttaaaatta tataaaccat taaatggatg taagcaattt
     8761 gaaggtgaac ttgtaggatt aacagaagat aataatataa aaatcattgc aaatggtaag
     8821 gaaatggaat ttaatagaaa agatgttgct attgtcagat tagcaataaa gttttagtgg
     8881 tatattaaat agggaggata taaaaaatga atcatgaatt tatggaagca ttagatgaat
     8941 tagtagttga cagaggtata gataaagaaa tacttataga tactatagaa caagctcttt
     9001 taactgcata caagaaaaac tttggttcag cacaaaatgt tagagttgaa tttgatagag
     9061 acaagggaga tataaaagta ttttctcaaa gagttgttgt tgatgaatca gatttatatg
     9121 atactttctt agaaatagag ttagcagatg ctagagagat aaaccctaat tatgaattag
     9181 gtgatattat agaacatgaa gttacaccta aaaactttgg aagaattgca gctcaaacag
     9241 caaagcaagt agttgttcaa agaataaggg aagctgagag agaaatagtg tataatgaat
     9301 ttatagaaaa agaaaatgaa atagttacag gagaggttgc tagagcaaat aaaaatgttg
     9361 tttatgtaaa tcttggaaga atagaaggta taatgactca aagtgaacaa ataccatttg
     9421 agaattataa agcaggtcaa aaaataaaat tctatatact tgaagttaaa aagactaaca
     9481 aaggacctca aatagttgta tcaagaagtc atccgggtct agtaaagaga ttgtttgaag
     9541 aagaagttcc tgaaatatat gaaggaatag ttcaaataaa atctgtagca agagaagcag
     9601 ggtcaagaac taaaatggca gtaagatcta tagatgaaaa aatagaccca attggagctt
     9661 gtgttggacc aaaaggactt agagtaaaaa atatagtgga tgaacttggc gatgagaaaa
     9721 tagatataat aaaatatagt gaagacccag cagagtttat atcagcagca cttagcccat
     9781 caaaggttgt tagagtagat atagatgaag atgaaaagtc tgccttagta atcgttccag
     9841 attatcagtt atcgcttgct ataggtaaag aaggtcaaaa tgcaagacta gcagcaaaat
     9901 tgactaactg gaaaatagat attaagagcg aatctcaagc agaacaagaa atgttaaatc
     9961 ttgaaaattc caaagaaata gaagctgagg atgttgaagc tgaatttatc gaccaagaag
    10021 agttagatac ttcagaggat aattttagtg aagaagagta aggagggatg attttgaaaa
    10081 aagtaaaaaa agtgcctcaa agaaaatgca tagcatgtca agatagagac tctaaaaaag
    10141 agcttataag aatagttaaa aataaagaag gaaaaatatt tttagaccca acaggaagag
    10201 ccaatggaag aggagcatat gtatgtaaaa gctctgaatg tctaaaaaaa gctataaaga
    10261 gtaaagcatt aaataaagct tttaaaatag atgttcctga tgaagtctat gataatctac
    10321 ttatggagtt agaagaatat gaaaaataat aaagaaaaag tactttcgtt cttagggctc
    10381 gcaactcgtg cagggaaaat agtgtctggt gatgattcaa ctttgcttga ccttaaaaaa
    10441 ggaaaagtaa atttgatttt aatcgcagaa gatgcatcaa ataatactaa aaaacttttt
    10501 aaagataaat caacttttag aaatattcca tatctatttt tttcaacaaa agaggaaata
    10561 ggatttgcaa taggaaaatc tccaagagca gttgtaggaa taaaagatga aaatttttct
    10621 aaaaagataa ttgaactaat agaaatttaa aataaaatag ggggtgatct tgtgtcaaaa
    10681 acaagagtat accaaatagc agaagaatta aatatatcaa atgaagagct tataaataag
    10741 ttagctgaat tagatataaa tgtaactgac aaagattcag tattagaagg tgaagagtta
    10801 gaattagcac ttgaaatgtt aggtgaagat ttatctcaag aaaatggaaa tgttatagaa
    10861 atagatggaa agttgacagt acaagtatta gcaacgaaat tagataaatc tccatcagaa
    10921 ataattatga agcttatgaa aatgggaact atggcaacaa taaatcaaga gataagtttt
    10981 gagattgctg ctttggctgc caaagactat ggatttgaat taacagtagc tgaaagtgat
    11041 gatacagaag ctttagaaat agaagcttta atggaaattg aagaagataa agaagaggat
    11101 ttaaaaccta gaccaccagt tgttacagtt atgggacatg ttgaccatgg taagacatct
    11161 ttactagatg caataagaaa aactgatgtg atttcaggtg aagctggagg aataactcag
    11221 catataggtg cttcagaagt taaaatcaat ggacacaaaa tagtattttt agatactcca
    11281 ggacatgagg ctttcacatc aatgagagca agaggagcac aagttacaga tatagcaata
    11341 ttagtagttg cagcagatga tggtataatg cctcaaacag ttgaagctat aaaccatgct
    11401 aaagcagcag gtgtaccttt aatagttgct attaataaaa tagataaacc aggtgcaaac
    11461 ccagataagg ttaaacagga attagcagac caaggattat tagttgaaga ctggggtgga
    11521 gaagtaatag cagttccagt atcagctaag aaaaaagaag gaatagatac acttttagaa
    11581 atggtattat tggttgctga aatggaagag ttaagagcta atccaaataa aagagcagta
    11641 ggaactgtta tagaagctga gcttgataaa ggaagagggc cagttgcaac agtacttgtt
    11701 caaggtggta ctttaacagt aggtgaccca atcgttgcag gtgttgcatg tggtaaagtt
    11761 agagcaatga taaatgcaaa aggtaagaga gtaaaaacag caggaccatc tacagcagtc
    11821 gagatattag gtctatcaga agttccacaa ggaggagacc aatttgtcga agttccaact
    11881 gataagattg ctagaagtgt agcagcaaga agacaacaaa tagttagaga tgaaatgtta
    11941 aaatcaactc aaagattatc actagatgct ctatttagtc aaatgagtga aggttctata
    12001 aaagacctta atatagttat aaaagcagat gtacaaggtt cagtacaggc agttaaacaa
    12061 tctttagaaa aattatcaaa tgaagaagtt caagttaaag taatacatgg tggtgttgga
    12121 gctgtaacag aatcagacat attacttgca gcagcttcta acgcaataat aataggattc
    12181 aatgtaagac cagttccagg tgcagaatct ctaggagaaa aagaaaatgt tgatataaga
    12241 acatatacta taatatacaa ggcaatagaa gatatacaag cggctatgac tggtatgtta
    12301 gaccctgaat atgttgatga agaaacagga aaagctgaaa taagagaaat atataaaata
    12361 tctggtgttg gaacagtagc tggatgttat gtgactaatg gtaagatatt tagaaattgt
    12421 aaagtaagat tagttagaga tagcataata atacatgaag gtgagcttgc agcacttaag
    12481 agatttaaag atgatgtaaa agaagttaat tctggatacg aatgtggtat gagttttgta
    12541 aattataacg acataaaaga aggcgatata gtagaagctt atataactaa agaagtagaa
    12601 agaaaattat aaattcgtat caaagaaaat cagttagtaa agaaggtcga tggtatggca
    12661 tcatataata gaacaagaag gatagcagaa gaaataagaa aagtcgtaag cactatgctt
    12721 ataaatggag taaaagaccc tagaataaca tctatggtga gtgtaactga tgtagaagtt
    12781 acaaatgacc taagatatgc ttatgtatat gtaagcattc taggtggaga tgaagaatcc
    12841 acactaacag gtcttaaaag tgctggagga tatataagaa gagaagtagg aaaaaatata
    12901 aaacttagat atattcctga aatagtattt aaacttgatg attcaataga aaaaggaatg
    12961 tatatggata gccttataaa aagagttaat gaaaaaaatg ctcaacaaaa tgaggatgaa
    13021 aattatgatg aataatatgg atagtattat aaactatatt tatgagggtg actattttat
    13081 agttacctct catatcagtc cagatggaga caatattggt tctacacttt caatgtatta
    13141 tacattgaag aatttaggaa aaaatgtata ttatgtactt gatgatgaag ctccattaaa
    13201 tttaaggttt ttggttgaag atgtaaaaat ctataagtct aatgaattta atatgaagaa
    13261 ctattctcta attgcacttg attgtgggga taagatgaga atatgtgtga gtgatgaaat
    13321 caaaaataat gcatcaaaat taatctgtat tgaccatcat gctagtaatg attcatatgg
    13381 agatttaaat tatgtagata ttgatgcatc ttctacatgt gaattagttt ataatttatt
    13441 agttagattt cagcaaataa aagatattaa tattataaat gaagatatag caacatgttt
    13501 atatacaggt cttgtaactg atactggaaa cttttcgtat tcaaatgtac atgcaagtag
    13561 ctttgaaatg gcaaaaaatt tacttgtttt aggtgcacaa aaaaatacta taatacaaaa
    13621 tatatatcaa agcaattctt ctggttatta caaattatta ggagaagctc taaagggttt
    13681 ggagatattt gattcaaagg tatctagtat agttttaact caagacatga tgaatagaaa
    13741 taatattagc tttaatgatg ttgatggaat aacaacatat acaagagaca taaaaggtat
    13801 agaagttgga attttattta aagagaaaaa acaaaatgaa attaaagtga gttttaggtc
    13861 aaaaaattat gttgatgtaa gtgaaatagc caagttattt ggtggtggtg gtcatgtaag
    13921 ggctgctgga tgtactatta gagatagtat tgataatgca aagaaaatgg tactagaggc
    13981 agtgttaaaa tcaatttaag gtgacagtaa aatgaataag attataagta tattgaaacc
    14041 cacaggtatg acatctcatg atgttgtaag tagagtgaga aagattttaa atataaaaaa
    14101 agttggtcat acaggtactc ttgacccaga tgcttcaggt gttttaccaa tctgtatagg
    14161 gaaagctaca aaagtatgtg aagttatttt aaacaaagat aaatcgtata tatgtgagct
    14221 tactttaggt ataagtacag atacttatga tgcttcaggt gaaattttaa aaaaagttga
    14281 tgattttaaa tttagtaatg aagacataga aagagctttt gacacacaaa gaggagagat
    14341 aaatcaactt cctccaattt attcagcttt aaaggtaaat ggaaagagaa tgtgtgattt
    14401 agtaagaagt ggtagacaat ctgaaattac tttaaaaaca agaagagtaa atataaaaga
    14461 cataaaaata ctaagtataa aaggtaataa agtaatgttt tatgttgagt gttctaaagg
    14521 gacttatgtg agaagtattt gccatgatat aggagaatat ctaggttgtg gagctcatat
    14581 gtcgtttttg aatagaacat cttctggtaa atttgactta gataattcaa taacattaga
    14641 agaattagaa ttattttatg aaaataaaac tttagacaaa tacttatatg atatagatta
    14701 tgtccttgat tcgtttaatt atgttgtatt aaaccctaat gctataaaat attatagtaa
    14761 tggtggctca atagatgata aaaggttttt aaagaataat ttcgataaag atgatgaatt
    14821 tgtaagagta tacagtacag ataatttctt aggattagga aagttatcaa aacacaataa
    14881 tacaataagt gttaaaagtg ataagatgtt catataacca atgtatttaa aaaaacaaca
    14941 aaagagatag tactataata acttctatag tctatctctt ttacttatgc tataatagtt
    15001 tgtgaataag ttaagagtaa attatatttt agcggggaac atttattatg gtgattataa
    15061 aatctataaa agaaatagca aacataaaag aaagtgtcat taccatagga aattttgatg
    15121 gtgtacacaa aggacatcaa gttcttatag gaaaaacagt tgaacatgca aaaaaagaaa
    15181 atatacagag tattgtattt acatttgcaa atcatcctgt aaattacttt agaccaaact
    15241 caacaaaaaa tattataagc aatgatgata aaataaaatt attagaagat ttgggaatag
    15301 atatagttgt aatcatacca tttgatgagt atatgactaa aatacctgca aaggatttcg
    15361 tagatgagat actagtaaaa aaattaaaag ctaaaaagat agtaattgga catgacttta
    15421 catttgcaag ggcaaaagaa ggcaatgtta atctattaaa aagcttagaa catgaatttg
    15481 gttttgaggt agaagtaata aaacccatca aaatcaatga tgtaagagtg agtagtactt
    15541 atattagaag tttagtatcc cagggtgata tggctaatgt caaggaatat ttgggtagaa
    15601 attacaagtt agaaggttgt gttatacatt ctaaacacct tggaagaact ataggattcc
    15661 caacggcaaa tattgacctt aagaataata tgctcgttcc aaagagaggt atttatgctt
    15721 ctattgtaca tattggagat gagacttatt ttggtgctac aaatgtaggc tataacccaa
    15781 cagttaatgg aaaaactttg tctattgaaa caaatatttt agagtttgat agagatattt
    15841 atggggaaaa tataattgtt gaatttttgg aaagaataag agatgaaaga aagtttaatt
    15901 caatagatga cttaaaaaat caattgtata atgatacaaa ttatgtgtat gaaaactatg
    15961 tttgcaaaaa caagtaatgt atgataaaat gattaaggaa aataaccatt actcggcatt
    16021 tgaatactcc aatcaatgct tagtaatagg cgtttctaaa aaaatacgga ggtaatctaa
    16081 atgataaaca aatcagaaat aataaaagaa tatggaagag ctgaagggga tactggttct
    16141 ccagaagtac aaatagcttt attaacagct agaataaacg agttaaatgg acatttaaaa
    16201 actcataaga aagaccacca ttcaagaaga ggactattaa aaatggttgg tagaagaaga
    16261 aacttattag cttacttaaa ggaaaaagat cttgaaggat atagagcatt aatagctaaa
    16321 ttaggattaa gaaagtaatt tcattcttta taaaaaagca ggtatagtgt atacctgctt
    16381 ttttatagtt aattcattat aaagtaaaat agttaataat aaaagtagtt tatatgttat
    16441 aaaaaattat aaaatgaata atattgggta agaatattaa aatagtatac aaatacataa
    16501 tcaatttaaa aggaaagaca ggataaatat agaaatttta taacaggata attagttgga
    16561 ataaggagga tattattttg aaaataaagt tagtatgcga tagtttgtgc gatattccag
    16621 atgaaatatc ggaaaaagat tatatagaga tagttccact tacagttatt tttaatgata
    16681 gggaatatat agaaggagta gatataaaga aagaagagtt ttataaaaaa gtaaaagaaa
    16741 tcaagcaaat acctaaaact tcacaagcta catatatgga gtttaaagaa gtgtttgata
    16801 agttcataac agaaggttat catataatct gtatgacagg ggctgcaaac gcatcaggaa
    16861 cttttcaaag tgctatgata gctaaaaatg atgtaaatga aaatgaaaaa atacatatat
    16921 ttgatactag aaatttatct ctaggtagtg gacaatatgt tataaaggca tgtgaacttc
    16981 ttgaagaagg actgggattt gaagaaatta tagatgaatt agaaaatact cgcagtagtg
    17041 taagattgct ttttgcacca tatacacttg attttttaaa gcagagtgga agagttccag
    17101 ttgcaactgc tttgatagga aatatgttaa atataaagcc tatatttttc tttgataatg
    17161 gggaagctaa gttggtaaat aaggttagag gaataaagaa tatagcaagt aaattggtcg
    17221 atataatttt agaaatgaat gaagggtctt tagaaggaaa aatcgtcaca ataggttgtg
    17281 gagataattt acacgattgt gaaatattaa aagatgaagt aaataaaaga ataaaagcaa
    17341 gaagagtatt atttactcga ggaggagttt ctatttgctc tcatacagga ccagatatat
    17401 tggctataag ttgttctaat tagtagtaat ataaggtttt tgcatatata tgtgaagata
    17461 attttatatt gttaatttat agtatttttg ataatatagt aaatagagtg atatacgtgt
    17521 tttttaataa gaggaggaca agcattaatg tttgaacata aaatatttaa aatggatttt
    17581 gcaggaagag aactttctgt agaaatagga aagatttgtg aaatggctag tggtagttgt
    17641 atagttcgat acagtgatag tatggtaatg gtaaatacaa ctaaatcagc caaacctaga
    17701 gacggaatag acttttttcc tttaagtgtg gattatgaag aaaaattata ctctgtaggt
    17761 aagatacctg gaggtttttt aaagagagaa ggcaagcctt cagagaaagc tatacttaca
    17821 tcaagattga tagatagacc aataagacct ttattcccaa aaggatttag aaatgatgtt
    17881 caggtagttg caacagttct atcagtagac caagattgta caccagatat agtagcaatg
    17941 ataggttctt caatagccct ttctatatca gacataccat ttaatggacc aacaggttca
    18001 gtatgtgttg gccttgtaga tggtgcattt gttgtaaatc caaacgcaga gcaaagagaa
    18061 aaaagttcaa tgcatctagt tgtttcaggt acaaaggaag caataatgat ggttgaagcc
    18121 ggagctgatg aagttccaga tgaggtaatg cttgatgcta ttttatttgc tcaccaagaa
    18181 ataaaaaaaa tagttgagtt tatagaaggt atagttgcag aagttggaaa agaaaaaatg
    18241 cctgttgaat tataccatgc aggagaagaa ataactcaat tggttcgcga atttgcaact
    18301 gataagatga aaaaagcagt tcagactttt gaaaagttag agagaatgga aaatatggat
    18361 agagttaaag aagaaacttt agctcacttt gaagaaactt tagaagattt tgaagatttt
    18421 gttggagata tagaagaagt attacaagat ataataaaag aagaagtaag aaaacttata
    18481 gtacatgaaa atgtaagacc agataataga aaacttgaag aaataagacc tatatggtgt
    18541 gaaactggca tgatacctag agctcatggt tcagccatat ttactagagg acaaactcaa
    18601 gtacttaatg ttgctacttt aggtgcttta ggagatgttc aaaaactaga tgggttagat
    18661 gaagaagaaa acaagagata tatgcatcat tataatttcc ctgcatatag tgttggagaa
    18721 gctagaccat caagaggtcc aggaagaaga gaaataggac atggtgcttt agcagaaaga
    18781 gctttattac cagtaatacc atctcaagaa gaatttcctt atgctattag attagtttct
    18841 gaagtattaa gttcaaatgg ttctacatct caagcaagtg tatgtggttc aacattatct
    18901 ttacttgatg caggtgtacc tataaaagat atggttgcag gtatagcaat gggacttata
    18961 aagcatgatg gtaaggttgc agtgctttca gatatacaag gtatggaaga ccatttaggc
    19021 gatatggact ttaaagtagc tggaacagag tatggtataa cagcaataca aatggatata
    19081 aaaattgatg gtatagataa agaaattcta caacgtgctt taaaacaagc taaagagggt
    19141 agaatccata tactaggaga gatgagaaaa actatatctc agccaaaacc agaactttca
    19201 ccatatgcac ctaagattgt taaaatgcaa ataaatcctg acaagataaa agatgtaata
    19261 ggacctggtg gaaagataat aactaagata atagacgaaa ctggagttaa gatagatatt
    19321 gaacaaactg gggaagtatt tatatcaggt attgagattg atatgattaa gaaagctcaa
    19381 gaacttataa ataatatagt agttgaacca gaagtaggaa aaacttataa aggtaaggta
    19441 agtagaataa tgaactttgg agcatttgtt gagatacttc caggaaaaga aggattactt
    19501 catatatctc atatagctca tgaaagagtt gccaaagttg aagatgtatt gaatataggg
    19561 gatgaagttg aagttaaagt tacagagatt gatgaaaaag gaagagttaa tttatcaaga
    19621 aaagttcttt taccaaaacc agaacataaa aataaataac ttattatata attaaaagca
    19681 aagtcttagg attttgcttt tttaaataca aaaaagtaga taattttaaa atgatagtta
    19741 tggatggctt atagttcttt ttctgtcagt ctttactctt ataaagaagc tatttaaaag
    19801 ctgattaata gaaagcttta ttgatatata tagaatcact taagttatat ttagtgcata
    19861 aacagagctt atttttaata gtattaaata agaaacaatt tgtacataat tagggggagt
    19921 tttaatgata atgatggtac ttaataagag taagctcaaa aaaatcataa tactaatttt
    19981 agtagtgtta ttagtattat caggaggggt aatggtatta aagttattta acaacaagcc
    20041 agtatttaat ttatttaatg gtgtagattt accatatgaa agaggtacaa aggataaaag
    20101 tggatatgtt gcattaacct gtaatataga ccttggatgg gaaacagagt acgtagaatc
    20161 cattttagaa acacttaaaa aagaaaatgt aaagattaca tttaatgtaa ctggaaaatg
    20221 ggcagaaaag aataaggatg aattattaaa gataaaaaaa caaggacatg aaataggtaa
    20281 tcatggatat aaacatttag attattctac attatcatat gaagacaatt atgagcaaat
    20341 agagacctct aaaaagataa ttgaagaaat tataggtgaa aaaactaaat tttttcaagc
    20401 accagcaggt tcctttggtc cagaaacagt aaaggctgct aaagcattag ggtatacttc
    20461 tatcaaatgg gatgccgata ctatagattg gaaatacaaa gaccaaccag aggtaatcat
    20521 tgatagaatg aagaaaaaag acattaaaga tagtagtata atcttaatgc atccaaccaa
    20581 cgcaacgaca aaatgtatag atgatattat agctattgta agagaaaaag gactaaaagc
    20641 aggaaaactt agtgatgtat ttaagtagac aaagtataaa tatttggtta agattgtata
    20701 agttgtagtt gaattaatta aaagactgac ttataatgta atacatataa aaaataaata
    20761 taattaaaac atagtgtttt caataatcca atatgaaaca aagaataaat tgttaggaga
    20821 atgtaatgta taagacaaaa atactggaaa atgggcttac aataattggt gaagaaatac
    20881 catatctaaa gtcaataaca ttaggtattt ggataaatgc aggctctagg atagaagaag
    20941 cacaggtgag tggaacttct cattttatag agcatatgat gtttaaagga accaaaaaca
    21001 gaacatcaaa agagattgct agttcaatag ataatttggg tggtcaaata aatgcattta
    21061 caagtaaaga atgtacatgt tattatgtaa aactaataga tgagcatata gatacaggaa
    21121 ttgatgtatt gagtgatatg atactaaatt ctaaatttga taaaaatgat atagataagg
    21181 aaagacttat aatattggaa gaacttaaga tgtatgaaga ctctccagat gacttatctt
    21241 atgatttatt agtggaaaat atatatgcta atgatggatt aggaatgaat ataattggga
    21301 caaaagaatc tctatacaat ataactagag aatccatgtt agaatactta aataagtatt
    21361 atattccaaa taacgctgtt atttctatag ctggaaactt taactttgat gatatggtgg
    21421 agaaaatcaa atcaaaattt ggacactggg aaaagaaaaa tttgagtata gacataagtg
    21481 aggctaagtt taatccttgc tttatatcaa aaaataaaga tactgaacag gtgaacttag
    21541 ctatgtgcct aaaaggtatt ccttttgaaa atgatgaaga agtctattca atggcagtgg
    21601 taaataatat tttcggtgga agtataagct caaggctttt tcagaaaata agggaagaaa
    21661 aaggtttagt atattcaata tactcatcac agacattata taggaaatgt ggtgaattag
    21721 gtatatttgc aagcatgagt acagaaaatt tacaagatgt atataattta ataaagaaag
    21781 aaattgaaaa tataagagaa aactatctta cagaaaaaga aatatctgaa agcaaggaac
    21841 agttaaaagg aaattatata ttggatttag aaagtactag cagtagaatg atgtctactg
    21901 gtaaatctat gttacttagt aaaaaagtaa aaactacaga tgaaatactt gaatgtataa
    21961 atagtgttaa tattaattca ataaagaagg ttgttgataa ggtattcaat atcgaaaata
    22021 ttggtacatg tatagttgga agagatgtag aaaagattct acacttggat taatattgaa
    22081 aaaagtaaat ctctggaggt ataaaatatg aatttatctg aaatagcagg taaggaaata
    22141 gttaacttgg tcacaggaga aagattgggg gtcattggag aatgtgacct tatactagat
    22201 gaaactacag gcggtatatt agcgcttcta attccaaaag aaaaaggatt ttttgccaga
    22261 agaaaagata agtcattttt agaagtacca tggagaaatg taaaaaaaat aggtaatgac
    22321 atgataatta tagaatatga gggatatatt taggaggaaa aaatgagtat tttagtgcaa
    22381 aagtttggtg ggacatcagt agaatcatat gaaaaaatga atgaagtatg taagatagta
    22441 aaagcataca aaaaaaatga tgaagaatta caattagtat tagtagtttc agcaatggga
    22501 agaaaaggag ctccatatgc tacagataca cttataaatt tgtgtagtgc agtaaatgat
    22561 gagccaagta aaagagaact tgatttaata atgtcttgtg gagaaataat atcaggaaca
    22621 attcttgcta atttattgaa tgcacagggg atagattctg ttttctttac tggtcagcaa
    22681 gcaggtataa ttacaagtga tgaatattct aatgcaaaga taaagtatat aaatcctaaa
    22741 aaggtaaaaa gagctttaga tgatggtaag gttgtagtta tagctgggtt ccagggtgta
    22801 acagatgatg gagaaataac tactcttgga agaggcggaa gcgatacatc agcagttgct
    22861 attggtaaag ctttagaatg tgaaacagtt gaaatttaca ctgatgtgga tggtataatg
    22921 actgcagacc ctagagtaga accaaacgct aaagtattga gttttataga ctatgaggaa
    22981 gtattccaaa tggctgacaa aggtgcaaaa gtaatacatc caagagcagt acagttggct
    23041 aaaagtggaa atataacttt agccataaag aatacaatga atccaacttt tgaaggtaca
    23101 aaaataggtt ctttatgtag acacttagaa gacaatatag aatatgaaca agaaagagat
    23161 tttaaggttg ctgttgcaaa taaagatagc gtagcacaag ttaagattaa gtcagcagaa
    23221 gaagtgttta cagaagtatt aaatgagata gaaaagaaac ttataactat agatatgata
    23281 aatttcttta tatcagaaaa agcatttgtt gtagaagatg ctgatataaa atcattaaag
    23341 gaaatactag ataaatttga attagattat gaagtaaata gagactgtgc taaggtaaca
    23401 ttgatatgtt caagaattgc tgatgaaatg tctggtatca tgtcaaaggt agttagagga
    23461 ttatcaaaag caggggtatc ccttttacaa acatcagact caaatatgac tatatcttgt
    23521 ttagttagtg aagaagatat gcatacagca gtacatgcta tacatcaaca attttattta
    23581 aaataaactg tataatacaa ttatttttta atcaaattta agaatttcat atatatctta
    23641 atatatttga tttatttggt aaaactaaat taaatataat tttgatagga gtttttttat
    23701 gaaagattat agagaagata atgaaagatt tgataaagat aattgtagtg aagaatctag
    23761 ttgcgaagag agagaatgta taaagcaatt tggtaccaat gaaatgccac ctcaacctcc
    23821 taaagatatt caatgtataa ctataattgg agaaattgag ggacatttta taggaaatcc
    23881 tcaaaaaaaa gctacaaagt acgagcatat aattccaatg ctatactcaa ttgaggaaag
    23941 taatgatgtt aaaggagtgt tagttgtttt aaacactgtt ggaggagata ttgaagcagg
    24001 tttagctatt gcagaacttt taaacagtac atctaaaaaa gtagttactc ttgttctagg
    24061 aggaagtcat agtataggtg ttcctcttgc tacagcagga gattactcat ttatagcacc
    24121 aacagcaacc atgattatac atccagtgcg aactactggt cttgtgattg ggataaatga
    24181 gacttttgag tattttaaaa agatgcaaga tagaataatt caatttataa taagaacatc
    24241 aaatatcaaa aaagatgtac ttgaaaaact tatgcatgaa aaagatgaac ttgtaagtga
    24301 tgttggtagt gtattaatag gaaaagaagc tgtggattat ggtcttatag atgaagttgg
    24361 aggactaaaa gaagcactta aaaaattaag agagcttata aaagaatctg aggaagaaaa
    24421 aaataatgat taaaatttgt ttaaataata aaatttatga tataatgatg taataaagta
    24481 ggagtgttag tgttaaggca ttagcacttt ttgctgtgaa ggggtaaaac ttgctaaaaa
    24541 taaggagtga tattaatggc cacgaaaaag aagaaaaaaa agaaggtaaa taccttaagt
    24601 tttaatgcag aatatcataa tttaataaca atatttatag gtgtattttt attatatagt
    24661 ttaaattcta attctatggg atggatacct gtgttaatgc aaaatctttt taagggacta
    24721 tttggagggc tttcaatagc aataccattt atagtaatta taacaggact tttaggattt
    24781 tttgatggaa atgaatatat atataggcta agaaagacta aactatacta cataattatt
    24841 ttatttatat ttgtatttta tggattatta aatgcaggaa ctcttcctgt agatagccca
    24901 ctaaaaggta acatgtttga tgatgttatg aaattgggtg taagtggaca aggtagtggt
    24961 ttgatagcaa caactattgc gtattatatg agtaaaatat ttggtatagc aggtggatgg
    25021 ttaataagta tcttcgcact tatactttct gtaatgttta tatttaatat ttctataaaa
    25081 gatttggtgt caaatgcaaa atcaaaatct aaagcatcta aagacagcaa tttaacgttt
    25141 aaagataaaa ttgcaaatat gaaaaaatct gcaatagata tgatgactga tgaagtagat
    25201 gatactacta taaataaaaa accaggtttc tttaaagggt taatgtcaaa aggtcttaat
    25261 aatgatgatg aagaagatga atattttgaa gaagaaaata gtgatggtgt agatgataaa
    25321 actataaaaa tagttggatt taataaggca gaagatgagt atttagaaat attagaagga
    25381 actcaaagta tgccagagtt agatgtttta aaagaattac aaaaagctac caatgaaaat
    25441 ccagttgtag atactaaacc agaaaaaaaa gttgatatag ctaaacctaa tttaaatata
    25501 gaaaaaactc aacctatgag tattgtagca gagccagtta atgaggatta ttcaaactat
    25561 aaaaagccta gcattgagct tttaaataaa gtaaataaga agtctgatga aaatggtaaa
    25621 aagaaagtgt tgaaaaatgc ttctctttta gaaaagacac tatcagattt tggagtggaa
    25681 gcaaaaatta atcaagttac agttggacct actataacaa gatatgaaat acaaccaagt
    25741 ccaggagtca aggtaagtaa aattgtaaat ttaactgatg atatagcttt aagtttagca
    25801 gctaaaagta taagaattga agccccaatt cctggtaaaa gtgcaattgg aatagaagtt
    25861 ccaaatgaag aagctcaaat ggttggtgtg agagaagttc tagaaagtga agaatttaat
    25921 aattttaact ctcctcttgc aatgggactt ggaaaagacg tagcaggaaa aattataatt
    25981 ggagatatag gtaagatgcc acacttacta atagctggtt ctacaggttc aggaaagagt
    26041 gtgtgtgtaa atacattaat aagtagtatt ttatataagg caaatcctga tgaagtaaaa
    26101 ttactattaa tagacccaaa agtagttgaa cttgcaaatt ataatggaat accacatcta
    26161 ttgataccag tagttactga ccctaaaaaa gctgcaaatg ctttaaattg ggcagttaca
    26221 gagatgaata gaaggtacaa attatttgca gatgctcaag taaaagatgt aacaagttat
    26281 aatgagaaag cggaagaaaa acttcctaaa atagtaataa taatagatga gttagcagat
    26341 ttaatgatgg caagtgcaaa tgatgtagaa gattatattt gtagattagc tcaaatggca
    26401 agagctgctg gaatgcactt gattgtagca actcaaagac catctgttga cgttataaca
    26461 ggggtaataa aggctaatat accatctaga atagcatttg cagtatcatc tcaaactgat
    26521 tcaagaacaa tccttgatat gggtggtgca gagaagcttc taggtaaagg tgatatgtta
    26581 ttctatccac ttggtgcagc aaaaccagtt agacttcaag gggcattcat atcagagagt
    26641 gagtcggaaa aagtaatcga ttttgtaaaa agtcaagtta aagatggaat aaaatatgaa
    26701 gaagatataa ttgaaactat atctaaggtt aatacttcaa agggtagtga tgaggatgag
    26761 tttttgagtg aagcgataga atttgtcgtg gaaagtggtc aagcatctgc ctctatgcta
    26821 cagagaagat ttaaaatagg attcaataga gcagctagac ttatagattc tatggaagaa
    26881 agaggaataa ttggagcaag tgagggaagt aaaccgagaa aggtattaat aagcaaacag
    26941 gatttgcaga atttagaagg tgaatagaat tgtcagttat aaaaatagaa aaagcactta
    27001 agctagataa agtaatattt gtagatgtga gaactgaagg agaatatgaa gaagaccata
    27061 tattgaatgc aatcaatatg cctcttttta aaaataatga acataatgac gttggaacaa
    27121 tttataagat gcagggaaaa catgaagcaa tacaaaaagg gtttgactat gtatcgtata
    27181 aattaaagga tatatattta caagcagcag aacttgcatt aaattatgat aatattgtca
    27241 tatattgtgc aagaggcgga atgagaagtg gcagtatagt aaatttatta tcatcattag
    27301 gtgtaaatgt atatcaactt gaaggtggat ataaggctta tagaaatttt gtattagagt
    27361 atctaaggca tgtaatggat acaaaaaact ttgtatctgt tcatggattg actggtacag
    27421 gaaagactga tttgttacat ttattagatg aaaaaggaat agatgtactg gatttagaag
    27481 gcatagctaa aaacagtggt tctacctttg gttttataac ttttgataaa aagccacctt
    27541 ctcaaaagaa ctttgaaaca aaactatttg aaagtatatt tttttcgaaa gagaattata
    27601 tatttgtcga gagtgaaagc aaacgagttg gaagtgttac agttccaaat gaaattttcg
    27661 aggcaatgac acgagaagga catcatatat tattagaatg tgatattgaa aatagagttg
    27721 atagactttg tagagattat atttatgata aagatgaagg taatatattg attttaaaag
    27781 aatgtataaa caagtttaga aaaagattgg gtaataaagt agtagatgat tatattgatt
    27841 tattagaatc aggtgaatat aaagaacttg taaaaagata tcttttagaa tactacgacc
    27901 cactttatat gcattcagta gaaaaatata agtatagtaa gattataaat tttaatgata
    27961 cacaaaaagc tttagaagat gttatagact tatatgagag tatattggaa ggagaaagtg
    28021 aatgttaaaa atagcattag aatcattagg atgctcaaaa aatttagtag atgcagaaat
    28081 aatgatggga attttaaata acaaaggcta taaattaatt ggagattttg aagaagctga
    28141 tgtaattata gtaaatacat gtggatttat agagtcagct aaacaagaat ctatagatac
    28201 gataataaac tttgcagagt taaaaaaaac tggcaattta aagttgttga tagtaacagg
    28261 gtgtctagcc caaagatatt ctgaagaatt aaaaactgaa attcctgaaa tagatgctat
    28321 agtaggaaca ggaagctatc aaaatataga taaaatactt aaagaattaa gtgaaatgca
    28381 taaaattgta agccttaatg atatagagtt tgtatttaat gaagatttac caagatatat
    28441 atctacacct agctatatgg cctatttaaa aattggagaa ggatgttcca ataattgtac
    28501 ttattgtata ataccaaaac taagaggtaa atatagaagt agaaaatttg aagatataat
    28561 taaagaagct aagaaattag ctgaatctgg agtaaaagaa cttgtggtta tagcacagga
    28621 tacgacaaag tacggttttg atttatatgg aaaagaaaga ttatcagaac ttttagaaga
    28681 acttgcaaaa atagatggat ttaagtggat aagggttatg tattcatatc cagaatctat
    28741 aacagaagaa ttgatacaag ttataaagaa atatgataat atatgtagtt actttgatat
    28801 gccaatacag catgcaagta acaatatttt aaaattaatg aatagaaaaa ctactaaaga
    28861 agatatatta aataaaatca atctgataag aagtaatata ccagatgcta tacttagaac
    28921 tacaataata gtagggtttc ctggtgaaac agaagatgac ttcaaacaac ttgtggactt
    28981 tgttgaagag gttaaatttg atagacttgg tgcatttgca tattcaagag aagaagatac
    29041 accagcagat agattgccaa accatataga tgaagaagta aaaatacaaa gaagagatac
    29101 tttaatgatg attcaacaaa aaatatctga agaacttaat gacaaaaaga taggaaaaac
    29161 ttatgaggtg ttaatagaag aacagataga agataatgta tatacaggaa gaacacaagg
    29221 tgatgcagag gaaattgaca gtatagtgta tgtaaaaagt gtagacaatc ttgaagtagg
    29281 agaatttgta agtgttcaaa taaatgatgc aatggaatac gatttaatgg gagatgtttt
    29341 atatgaactt gcctaacaag ttaactttgt ttagaatatt tttaatacca gtatttgttt
    29401 taattatgtt gctcaatata ccaaataaat ttttaatagc ttgtattatt tttataatag
    29461 cttcaattac tgatgcttta gatggaaaaa tagctagaaa atacaatcta gtcactgact
    29521 ttggaaaatt tatggaccca ttagcagata agttattggt tatttcagca cttacatgta
    29581 tgatagaaga ccatcttgta agtagttgga tggtaattat aatagtggct agagagctta
    29641 cagttagcat attaagagct atagctgctg ctgatggaaa agtaattgct gctggaaaca
    29701 gtggaaaact taaaacgata actcaaatgg tatctatagt gtttttattg ttaggtgctc
    29761 agtttgaaaa tgtattgata ttaaatattg gtgaaattct cattcttata gctacactac
    29821 ttacattata ttctggatgg gaatatctat ataaaaataa agagcttttt atgtcttcta
    29881 agtaaaaaat tatgctttaa taaataaaaa ctaatgatta ataaataaaa aataacaact
    29941 taaaatttta aaaatgta
//
LOCUS       DAMJZE010000029        29147 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJZE010000029 DAMJZE010000000
VERSION     DAMJZE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.029"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            123..2765
                     /gene="adhE"
                     /locus_tag="PC541_001206"
                     /gene_synonym="adhC"
     CDS             123..2765
                     /gene="adhE"
                     /locus_tag="PC541_001206"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2838677.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3125..3466)
                     /locus_tag="PC541_001207"
     CDS             complement(3125..3466)
                     /locus_tag="PC541_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838678.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3493..4164)
                     /locus_tag="PC541_001208"
     CDS             complement(3493..4164)
                     /locus_tag="PC541_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838679.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4176..6752)
                     /locus_tag="PC541_001209"
     CDS             complement(4176..6752)
                     /locus_tag="PC541_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2838680.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            6960..7895
                     /locus_tag="PC541_001210"
     CDS             6960..7895
                     /locus_tag="PC541_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2838681.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7914..8651
                     /locus_tag="PC541_001211"
     CDS             7914..8651
                     /locus_tag="PC541_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838682.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8836..13893)
                     /locus_tag="PC541_001212"
     CDS             complement(8836..13893)
                     /locus_tag="PC541_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2838683.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(13918..14337)
                     /locus_tag="PC541_001213"
     CDS             complement(13918..14337)
                     /locus_tag="PC541_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838684.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14571..14822)
                     /locus_tag="PC541_001214"
     CDS             complement(14571..14822)
                     /locus_tag="PC541_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838685.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            14911..15642
                     /locus_tag="PC541_001215"
     CDS             14911..15642
                     /locus_tag="PC541_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2838686.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15701..16612
                     /locus_tag="PC541_001216"
     CDS             15701..16612
                     /locus_tag="PC541_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2838687.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16663..17475
                     /locus_tag="PC541_001217"
     CDS             16663..17475
                     /locus_tag="PC541_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2838688.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17584..18276
                     /locus_tag="PC541_001218"
     CDS             17584..18276
                     /locus_tag="PC541_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2838689.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18363..19304
                     /locus_tag="PC541_001219"
     CDS             18363..19304
                     /locus_tag="PC541_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838690.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19341..20498
                     /locus_tag="PC541_001220"
     CDS             19341..20498
                     /locus_tag="PC541_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2838691.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20650..21210
                     /locus_tag="PC541_001221"
     CDS             20650..21210
                     /locus_tag="PC541_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2838692.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21238..21729
                     /locus_tag="PC541_001222"
     CDS             21238..21729
                     /locus_tag="PC541_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838693.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            21958..22509
                     /locus_tag="PC541_001223"
     CDS             21958..22509
                     /locus_tag="PC541_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2838694.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22595..23527
                     /locus_tag="PC541_001224"
     CDS             22595..23527
                     /locus_tag="PC541_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2838695.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23582..24445
                     /locus_tag="PC541_001225"
     CDS             23582..24445
                     /locus_tag="PC541_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2838696.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24692..26554
                     /locus_tag="PC541_001226"
     CDS             24692..26554
                     /locus_tag="PC541_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2838697.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26557..28023
                     /locus_tag="PC541_001227"
     CDS             26557..28023
                     /locus_tag="PC541_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2838698.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28047..28883
                     /locus_tag="PC541_001228"
     CDS             28047..28883
                     /locus_tag="PC541_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2838699.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10768 a         3492 c         4659 g        10228 t
ORIGIN      
        1 aaaatctaag gtatagagat gaaatctata aaattatttt aagtataagt taaaaatata
       61 gatgatgcat ttaagattta taaaaattag agatgcataa ttattttagg gaggttttta
      121 gtatggagaa aaaagaaaag gtagtagaaa aatcaaatgt tgaagtatgt agtcctgaaa
      181 ttgtcaacag tgttgaaaca ttaagaatga gattagaaga aattagattg gcacaaaaag
      241 aatttgcaac atttacgcaa gaacaggtag ataagatttt cttagctgct tcaactgcag
      301 ctaatcaaca aagaatacct ctagcaaaaa tggcagtgga agagactggt atgggtattg
      361 ttgaagataa ggttataaaa aaccattttg cctcagaata tatctataat gcttataaag
      421 atactaaaac ttgtggtgtc atagaaaaag atgaagcatt tggttttaca agaattgctg
      481 agccagtagg tgttttatca gcagttatac caacaactaa tcctacatca actgcaatat
      541 tcaaatccct aatagcacta aaaactagaa atggtataat tttctctcct catccaagag
      601 ctaaaaactg tactatagaa gcagctagag ttgtacatga tgcagcagtt aaggcaggag
      661 caccaaaagg acttataggt tgggtagatg ttccttcgat tgaattaact aatgttgtca
      721 tggcagaggc agaccttata ttagctactg gtggacctgg aatggttaaa tctgcatact
      781 cttcaggtaa accagctgtc ggagttgggc ctggaaatgt acctgcaata atagatgaga
      841 gtgcagatat aaaaatggct gtaagttcaa tcttagtatc aaaatcattc gataatggta
      901 tgatatgtgc ttccgagcaa gctgtaatag taccagaaaa aatatatgaa gaagttaaaa
      961 aagaatttaa atatcgtgga gcacatttcc taaataaaga agaaactgaa aaagtaggaa
     1021 aagtagtaat aattgatggt tcattaaatg ccagaatagt tggacaacca gcacatgtaa
     1081 tagccaaaat ggcggatgta gaagttccaa aaacagctag aataataatt ggtgaagtag
     1141 aatctgttga attaaatgaa ccttttgcac atgaaaaact ttctcctgta cttgcaatgt
     1201 acaagtctaa aagttttgaa gatgcagttg ctaaagcaga aaaattagtt gcagatggag
     1261 gctatggtca tacatcttca ttatatgcag actctataaa tcatccagag agagtagaaa
     1321 agtttgtaaa tgctatgaag acttgtagag tattagttaa tactccatca tcacaaggtg
     1381 gtataggtga tttatataac tttaaattgg caccgtctct tactcttggt tgtggttctt
     1441 ggggtggaaa ctcagtatct gaaaacgttg gagtaaaaca tttgcttaat atcaagacag
     1501 tagctgaaag gagagagaat atgctttggt tcagagcacc agaaaaggtt tatttcaaaa
     1561 aaggatgttt aggtgtagca gcaagagaat ttaaggatgt tatggataaa aagaaagcat
     1621 ttatagtaac agattctttc ctatataata atggatatac taagaaatta actgatttat
     1681 tagatgagat gggaattaag catactacat tctttgatgt agctccagac ccaacattag
     1741 cttgtgcaag agaaggagct aaagctatgg ctgatttcca accagattta ataatagctg
     1801 taggtggagg aagtgcaatg gatgctggta agataatgtg ggtaatgtat gagcatccag
     1861 aagtagattt ccaagactta gctatgagat ttatggatat aagaaaaaga gtatatgtat
     1921 ttcctaagat gggtgaaaaa gcgtattttg ctgctatccc aacttcagca ggaactggtt
     1981 cagaagtaac tccatttgca gttataactg accaagattc aggtgtaaag tacccattag
     2041 cagattatga attaatgcca aacatggcta taattgatgc tgatatgatg atggagatgc
     2101 caccaagatt aacagcagca tcaggtgttg atgctttaac acatgcatta gaagcatatg
     2161 tatctatgtt aagaactgaa ccagctgacg gtctagcact acaagcaggt aaaataatat
     2221 ttgaatattt acctcgtgca tataaaaatg gaaagaatga taaagaagca agagaaaaaa
     2281 tggcaatggc atctactatg gcaggaatgt cttttgcaaa tgccttctta ggaatatgtc
     2341 actctttagc acataaatta ggtgcattcc accatgttca acacggtgtt gcaaatgcat
     2401 tattaataaa tgaagtaatc aagttcaatt gtgcagaagc tccaaataag atgggtgcat
     2461 tctcacaata cagatatcct gattgcatac aaagatatgc tgaatttgct tcttttgcag
     2521 gtattaaagg aagtacagac caagaaaaag tagataatct tataaaagca atagatgaac
     2581 taaaggcaaa agtaggtctt cctaagacta taaaagaggc tggagtagaa gaatctaaat
     2641 tcttagaaag attggatgct atggttgaac aagcttttga tgaccaatgt acaggtgcta
     2701 atcctagata cccattgatg agtgaattaa aagagatata tctaaaagtt tattatggta
     2761 aataatatct attgtgcaac ataggttcta ttgggtttgt atataaatta ttagatttga
     2821 atataaatag agttttatta gagacacgta gaagtaacta taaataatag attataattt
     2881 aaattctaaa tggaaatgat aaaaagtcag taatttaatg gtaatttcga atatgtgtat
     2941 ttaatcaatt aactttaaaa ttagtcggaa aattttttta ttacatataa ttttaaagca
     3001 taatatataa aaaactttat gaaaaaatta taaaaatatt cagaaaattc tattgactaa
     3061 tttatttatc atgatataat aagtttagaa aagaaaaaaa ttggggccta ttaaggcccc
     3121 cttactattt tttgatagat ttatttaaaa ttactttctc attttgattt tttatattta
     3181 ttatagagtc agtataatcc acttcaaagt tggcaaaagc agagaataaa tctatatctc
     3241 ttaacaaatg aattgtcata cattgattgt tcttatttag ccttagaatc aatgctctta
     3301 caaatggaaa ttgtattcta gaattaaata agttagaacc atcagtatct atatctaagc
     3361 tacgataatt aatatctata aatatatcta catcttcatt ttcttctctt attattctgt
     3421 agtcaaaatc caagtttaaa tttaaattac ctaaaatcaa taacatttaa atcctctttt
     3481 ctataaatag ttttagagtg ttaatttaag ctctccatct attatagata taattctatc
     3541 tgcttttcta gcaatatttt cattatgtgt tattattata agtgtttggt tatatttttt
     3601 tatagaactt tttagtaaat ccatgacttc ttctgtagtt ttactgtcca aatttcctgt
     3661 tggttcatct gctaggatta tacttggttt tgcagataaa gcacgtgcaa tagcaactct
     3721 ttgctgttga ccaccagata attggttggg aagatgattt ttcctttcat taagacccaa
     3781 taaatttata aggtcatcaa tatattcttt atctattttt ttattgtcta actttagtgg
     3841 taattctata ttttccttta catttaatac aggaattaag ttatactgtt gaaagacaaa
     3901 tcctaccttt tgtcttctat atcttgctaa ttctttatct gaaagacaac atatatcttt
     3961 accatctacc tttatagtac cattacttgg tctttcaaga ccacctacta aatgtagaaa
     4021 tgtacttttc ccacttccac ttggtccaat tatagcaaca aattcgcctt tatttatttt
     4081 taaatttgca ttctttagtg catgtacaga tgtttccttt tttccataaa tcttacctaa
     4141 gttaatagtt tccaatatag gcataattat tcctcctatt ctgtaatgtt tatagcttca
     4201 actatactaa ttttatttat ctttcttgat ggaatatatg tagctagaat acctacaatt
     4261 atatttgcta caacaactag tatatatatt ttgtaatcta ttgaaaatcc caatccatat
     4321 gatacaaagc cctgcatata atacatttta aattgtatta taattcctga cacgatagta
     4381 attatacttg agagtacacc atataatatt ccttcataaa gtatcatatt tttaaagcct
     4441 ctctcagaga taccaatagc tcttaacatt ccaaattcac tagttcttga agttaagtta
     4501 taacttatgt tgtttattat gttaagtaca ctgattatga acattattgc tactatacca
     4561 taactataaa tcatagttct agatgtcatc ttatcagaat tttctttttc taccatcata
     4621 tctactgttg tagttccagg aacttcactt ccaattttac ctaaaaggtt atttattttt
     4681 ttcttgtttg cattttcatt aaggtctgca taaactaagt tatacttatc tacaccagtt
     4741 aattttttta ggtattcgct actcgttatt acatctacac catcatctcc agagtacata
     4801 ccattatctg caaaaggata acttataata gctccaactt taaattcata ttctaagtat
     4861 tcataattat cttttagact ttcataaaga cctgtatcca tcccaccctt tggatatttg
     4921 acttttatag tatcacctac ttttatatct acagctggtg ttccttcacc aggagtaaaa
     4981 gtctttgtgt atttctgtgt tttagaaatt tctggtacat atacaatagc taaattttct
     5041 tttttcattt ttttcaagtt tatatttcca cttactaagt atttttctaa tgactttatc
     5101 atctcatcat tataaccttt tagcttttgc tttagtgtat atttgccagt agtcttattt
     5161 tttatgaaca gaggatttag cttataataa ggagttgaat ttatagcttc gtagtaatct
     5221 aatctattta ttttatttac ctctattctt gtgtttaaga tactagcagt tttgactttg
     5281 tcaattccat ctatattttt tattttatct atctgattat cattaaaccc ttttatatct
     5341 ttaaaagaag tattgttacc aatttcattg agataaacat ttgatagaat aaaatctgca
     5401 ttcatatatg tttgttcttg agtagatttg ttttgcacat ctaaaaaaga atatttataa
     5461 ttgtctttta tgacgattat acctcctata ctcatagata atacaattat aatgaacccc
     5521 tttttatttc taaagatgtt tttcgcagat atagattttg ttactgacat atttaaagtt
     5581 aattttgata ttaaataatt aactttggtt acatttttat cagctccaaa agtttttctg
     5641 atagcatcaa taggagaaat tcttcttatt tttaagtatg taaagaaact tataattagt
     5701 attattagta ttgtacatgc aattgataag agaattattt tatctggaat aacaaaaggt
     5761 gtaacattta tatttccttc aaattgaata tttcctacag acctattaaa tatttgtgca
     5821 cctcccatac ctatacatat acctataggc attgctatta gagcaagtat gaaaagttca
     5881 taaagcataa gtttgaaaat cctaaagttg gtggaaccaa tagctcttaa gacgccatat
     5941 tcctgtactc tttggtatat tgatataaca tagatactat atattacaat accagcaaat
     6001 agactcatag caattgcaac gtatttactt gaattatcta aagtaccgtt tttcatagta
     6061 gattctataa gcattgagtt tcttcctata cttttctcat ctaacatagc attttttatt
     6121 atattgtcta tgtttgtatt tatatcactg ccttcattaa attcaatata agttgtaaaa
     6181 tcattcagtt ctgattcatt gagattagaa aaaatttcac attgtccctt ttttttctct
     6241 ataaatctat cttcaaggat tccaacaact ttaaaagttt ctggattttc tttttgatat
     6301 aatttaaaag taaggttttg atttatctct ggttttaagc ccatactatt taaaatccat
     6361 ttctcgacta ccacctcatt gctagctttt ggaaatcgac cttttataag tttagaatga
     6421 ctagtcaaat aatttttatt tgtatatagt atgtttatag gtaatttttc attaacatct
     6481 gtagacgcat agtaagatgt tatacccaga tttttaatat ctttgctaga ttttatggac
     6541 tttaattgat ttttctttat atttttaaag tatacatggt cagttccaag ctctcttttc
     6601 aatctatcta catctgcttg ctgagcactt cttgagagag taccaatacc aacaacgagt
     6661 gatgtagcaa gaattatact cacaacaatt gctaaagacc tagctttata actttttata
     6721 taccttagta ctattttcaa attatctttc atatttacca cttcctttct taactaaatc
     6781 aattatatat ttatctaaaa tatatagcaa agtaagtatt ttcaaatttt aagtagttaa
     6841 ttgtacaaat tcatctgtat ttttacaagt ttaattaata tatatgtaaa ttttaacttt
     6901 ttacataatt tttttaaaat gatataatcc ctatataaat tgtagagggg aggggaatca
     6961 tggataacaa ttttgtattt cctatagtaa tactaacttt gtttatattt agcaatctta
     7021 taatgaataa gtataaattt tacaaagaat tagacatatt aaaaagaggc atattaacat
     7081 tttttatatc gtgttttgca ttgattttat ttatggaggg cactaaatat atacaattaa
     7141 ggttaggata tagtttttta cttgaaggag tattaaatat tatacttttt ttggttttaa
     7201 ttattttaag tttttataca ggctttaaag ttataagatt gataaataat aataatgaac
     7261 aggaatataa attacaaaaa ctagatagta aaataaatca taaggataat ataattatca
     7321 aattaagaag tcaaaaacat gactatttga aacatttgca agtagtgtat tctctattaa
     7381 atactgggct agatgaagat gccaaagaat atataatgaa tatatcaaat agctttaaat
     7441 caaaaaattc aaggtatgga aaagtatctt atttagatgc tatagcttca ttaaaatacg
     7501 aggaatgtct tgaaaaggat atatcttttg atgtctacat tcaagatttt atggataatt
     7561 tagagattga acctaatgat atgagttcag tactattaaa cataatagat aatgcaatag
     7621 agtctttgtc tactataaaa aaagaaaaga agtatataag attacatgca tatgaagatg
     7681 aatttcagta tattatagct ataaaaaata atggtcctaa aatacataat gttgaaagta
     7741 tattttggaa tggatttagt actaaagaag gacaagatag gggatatggg ctacatttag
     7801 ttaaaaatat attagataag tatgattata gtatacttgt aaatagtgat gattttttaa
     7861 ctgaatttgt aatcttaatc ccaaaatatt attagttagg aggagaaaag tctatgataa
     7921 aagttttgat ttgtgatgat gataagattg taagagatga tatagcaaat atagctaaag
     7981 agtctggata tgtagattat gttaaaaaag ttaaggatgg tatagaggca attgaattta
     8041 taaaaaatga gaaaatagat ttattgatta ttgacattga tatgccatat aaaaatggga
     8101 ttgattctgc tgaagaaatt ctttctatag ataaggatat acatatagta tttgtgacgg
     8161 gtttcgctga ttatagtctt gaaagcttta aagtgcatcc tatagatttt gtagttaaac
     8221 catttaaaaa agaaaaaata cttgattcaa ttaacatagc aatagaccat attaactccc
     8281 ataagattgc taataataat tttatagaag acaccctttt tgtatataaa ataaggaagc
     8341 agatacatat gattaatttt gatgatatag ttatgtttga gaaaaattca agagcaatta
     8401 atctacacac taaagatgaa gatataataa agttttatga gaactttgat gatttaaaaa
     8461 aacgcctacc tcctaatttc tttatgaccc atagacaata catagtaaat ctaagattta
     8521 tacataaggt tatacctata aataaatctt cattagagat aaggtttata aattttcaag
     8581 aaaatgccat attaaacaaa tctctagaaa aagatttttt atatagattt tatagggtta
     8641 aaagattcta gtgaagatag aagaaaaata ctttttgtgt gatataataa gtaccgtaaa
     8701 gataatgtat aagagaagtt tatataaaaa ttatttgtaa ttatgattta atctatataa
     8761 attacagtat agtatatatt ggactttcta atttttttaa gaaagtccaa tatattttag
     8821 agatagtttt tatacttata aattacttaa gatactaggg tctttaatct tactatcttc
     8881 agcaagtaat agtatcttag acataatctc agcagtttta gggtcttcat cagcgaatgg
     8941 aaggaatatc ttacctctac tttgagaatg tataggaagt atattcaaag cacccatccc
     9001 ttttttatgt acaataccag aacccatatg cacagaatac tcacctaaat tacctgctat
     9061 aagagcatga gagccttcaa aattgacatt gttaattttt aataataata aaagttcttt
     9121 agcaattgct atacgcatct ctacagtaga atggctagtt tcagggtcaa caccaccagc
     9181 atgagcgaca cttacaacaa ggtctatatc acgcataact tctgagaata gaattggagg
     9241 tatagaagct aattctaaac ttttataagt ttttctatca aaaaattcta ctgtttcaat
     9301 agtaggaggc tctatatctg ctggtgaaaa ccaatctgca agtgcaaaca tctttgccac
     9361 tatattttct ttataaaata ctttctgtaa tccctcttca taatcgacag tccaaagctg
     9421 atttctaaga agtgctacag ttttctttgg ctgaatttgg tgaccagcat atctgcgaga
     9481 tatagttttt tcagataatt catcactgtt tattctataa tactcacgga atacttgctt
     9541 aaatggttga acaatttgct tactaaataa atcatgttgg aactcactcc acttaccaga
     9601 ttcaaacaaa tgaactggat gtgcaagttt taatttattt tttgccgtca acttataagt
     9661 ttcaccttct atattgcaaa taaagaactt agacttatca ttataaacat atccaatatt
     9721 atcatcacat acccatacta gatttttaat caaagggcag ataactggat ttttatagat
     9781 attggcaatt tctttaactg taaatactat ttcatttttc atagcttgtt ctaaactctg
     9841 ttttgcacga gaatactgac tctttaaatc tttttggaca gatttaagtt caagtacata
     9901 tggatgttta gcaatagctt ttggtatact ctttaaagac ttgttgcctt tttgaacttt
     9961 agtagatgct tttccgtttt catcaacatc tatccaaatt gaagtaccat caatatcttt
    10021 tggtttaaat agatgagcaa cttcatttag tttttcagtc tcaagagccc acatcatacg
    10081 atttacatct ccaaatccag cacttctagc tagattttct agcgctatag tacaagcttt
    10141 tgattcactt tcacgacgtt gtgcaccaaa ttgcttgctt tctttagcaa acttttgaat
    10201 aaattcataa cggaaagttg cttcttttag gtctcctttt ttcataggaa gtaaagagaa
    10261 acttctcaat ttatcttgat ttctatttgt ttcaatttct ttcatcaaca tgtctttatc
    10321 aagcttacca agtgtagcat cagcatagag ttgtgcacgt ctatgagagt taccaccact
    10381 agttatatat ttagctgact tgtaaagtat attgaatctt tcttccccaa tagttttata
    10441 tgaatctaaa aaccaatcta catcaaatgc accatcaatg aaactacgtg gtgaaatagg
    10501 ggagaataga gcaacctctg attctttttg tgcagagaag ccttcattta tatgtgcatg
    10561 gaagtaccaa attgtacttt tcagaccctt ccagtttaaa tatttttcaa ttatgtttac
    10621 ccattgaggg gcatacatag cagattctat caatcttaac tcagtaatgt cggtattttt
    10681 aagtaaactt tttaattttt ttgaatcatc ttctttagta ggatagcaat ttttaagtag
    10741 agaacttaaa acatcttttt ttgaataatt gctaccatat gaatatcctc tctggaaagt
    10801 ttctttacca agagcgacca aaatatttac aaaataattt ataccttcaa agtattgtat
    10861 attattagct agattagaca cttctgttgg catatcacct ctacgtgatt caatttctat
    10921 aattcggttt gtaacagttt ttaaattatc tttaagccaa gggtactttt ctaatgtgtc
    10981 gccagcccat ctaggatttg tcaatgttct tattatattt gatgcgttgt atccacttat
    11041 catttctcta ttaacttgct cttcagtcat taatcctaat tcatgtgcac gagcaaacac
    11101 atttatgcta gtatagaaag gaaacttata ttctagcaac tgataatttg aaaacgtagt
    11161 gtagaaaaac ttagtaaaag aaatatcatc ataaataaac gaatgtgctt tatcaagcca
    11221 atagtttaaa ataggggcat ttaaagcagg gcagtattca ttttgtctgt aatagttttt
    11281 agtaaattca actctttgtt ttttacattg ttctatagtc aataagtttc taagtttata
    11341 gtatatttct aaacaatagt taaatatttc tttatggtca aactcattta gtaaaactct
    11401 taaaagtttt cttgtaagtc cattaaattt aagtttttca agttttgtat atattttatt
    11461 ttgtttttta gagtcaagga catttgaata tatagatgaa aaccaacttt tttctgtttc
    11521 attatatcca catgtactta tactataaaa taagatatca agtattaatt tgtaatctaa
    11581 gtcaagtttt ttactagttt ctctccaaag ctctgctaat ggataattat caagagtatc
    11641 attagaaact ttatatttaa taggagtcaa gttataagta gaataaccta aaagtacttt
    11701 gtctacatta cctgaccatc tttcaacttc atattcataa tctgcatttt cagtaataag
    11761 ttcatcaaat ttttcataaa cttttattat tttatttata tccgtatcga gaattttttt
    11821 tgcttctttt tttgttgcta cagaaacaga atcctcacga gtagataaac ttatatttga
    11881 gataatttta ttaagctttg atgagctatt agaatcatct ttaaaatcaa attgttctgg
    11941 aatttgaact ttgttttcag ggttatatat tccaaaacca ttttctttgc agtaatcatt
    12001 ttttgcttta cctaacaaat tatcaattag tatttgaact tgagatgtat tctttttaga
    12061 tttatcaata tcctctataa gtaaaataag cttattatat agttcttgtc tagcagtatt
    12121 gtcttttaat aagtttacaa tttcaagacc tccaaggcat ttttgagaat ttgaagattt
    12181 tatcagtgaa gaagcaatat tttccaaatt tgaatcatct tgttcaagta aaatttttat
    12241 tactgtttgt ctaatagagc ttgtctttaa agacaataga ttggttatct tatctaattc
    12301 tacatctgtt agctcaagct ttctaagtag actcaatacc gtttctctat tggaaacact
    12361 cttatcactt aaacattcca ataaaaattc acgttgtcta tctgtctttg gttcatttac
    12421 aaaattgact ataagtgaag ctcttaaatc tgaactcata agatgacaat tttccataag
    12481 cacatctata aattgagcat tcatatcgta tgctgttaaa gtcatcatac atttaagtat
    12541 ttcatgagaa gaaatttcaa tccaagtcca tggaaataca cttcctgaaa atgattttgt
    12601 ctttgaattt aaaatttcta aagtatgttt taaactttca aattgttctt ttctagcatc
    12661 aaagtctttg tcaataggag tgtttgacaa gtcaaaatta gtttctctgt tttgccaact
    12721 atagtagtag ccaacagaac cttctacata gaaatttgta attacccatg ctagtatctc
    12781 taaatcatca cccttaatgt ttttagatgc tatagaatgt ttaaaatatg tactttctgt
    12841 atgcgataaa aagtaaagag ctacaagttt tttatatttt tcctgacaag aaagtaagtt
    12901 ttcaataaga gagtaagagt catatatgtt gtaagttgca gtagcccata aggcaaaata
    12961 aatttcaaga ttatcattgc tattaattga ttctatacgg aacttttcat cagtcaatga
    13021 tttatatgaa agttctaaac atttacgtat tattccaggt ttttggtctg aaaaaccaag
    13081 tcctgtccat gtatccaagg ctcttataac agaactaaat cgctctaaat tgttgtccaa
    13141 aatagttttt aacatgtata ttgatgcaga aattgtaccc tcatctaaac tctctacaat
    13201 ttgctgtcta agtccttctt gttgtttggc tgccaaaaga agtttagaaa gcatttcata
    13261 gcatttatca ctatgactct tcatgatacc aattatcatt tgtcttgtaa gtagagcagt
    13321 ttgattttct ccaaatatta cttcttctaa agcacttatt actttttgat ttcctttgtc
    13381 tatttctagt gctattttat cactcattat agagtcatag tacaagacct tatttggaag
    13441 agtaagataa tctataactg aaaaatcata ataaattaaa tcaacatagt ttgaaataaa
    13501 ccttataata cctctaaaat aatccacaag tttatttgag cgataccctt tacgataata
    13561 tcctcctgaa tatgtataat ccatctttct tttaattaat tggcaaagtt cttctacttg
    13621 ttcagaagta aatattaatt ctaatgcatt ttttaaaggt ccagaaagga gctcgtccat
    13681 atttttaatt tcgtagtagt ggcaattgta gtatttcttt atcatattta agaatttgtc
    13741 ttcaggttct ctataatagt atgaactatt ttctatgcag tcccaaagag actttgctaa
    13801 gtcgtagtac tgttttgaag aagatttagt aaaattattt ttaaaatttt caattatttt
    13861 aggatttttt tctactctag tacgttctaa catttatgta cctccaaatt atttatttta
    13921 aaacattctt ccagcaagca aggtaagtct tataaaagta aatatatctc cacttttcag
    13981 atttatagag gaatcgagtt tttcaataac atcctcattt ataagcactt tgtagattcc
    14041 atcttcaaat gcctgtaacg ctacctctat tgccttttct acagattgtt tattctcatt
    14101 gtaaatagag ccaaatccaa cttttccaat atcagcttga gattcaattt cttcattagt
    14161 taagaagttt attattgatt tttctgggat tttctcatta tattcaagaa catttttaga
    14221 tacaatttca gagatagctt cttttaggca attgatgttg tcagatacat caaaaggggt
    14281 aagttctagc ataggtttgc gtttgccaac agccttaact ctaaaataaa tttgcataaa
    14341 atcacctcgc aaaatattaa ttatgtattt agaaatttaa aaatttatct atttgttata
    14401 ttatatcatt ttgtgaatta ttaatgtgaa ttatggaatt attttacaaa aaatagaaaa
    14461 aaagctatct taaagtctaa aaaagcaata aaagataact tttaactata attatataga
    14521 attataactg tacatttatt aaattttaat tttagaatta tttttgataa ctatcttttt
    14581 ttatttataa attttgctcc taaatatttt ttgatatctt cttcaggaga aaagtcatca
    14641 ataagtattt ctttcccagt gtcttcaaat actacaagaa cttcacagtc accttgtaag
    14701 aaaaattttc taactgtatc ttctttaact tctctaactt ttattacatt ctttttttct
    14761 ttttcaaagt cttttgtgta ataacttccc atcatattaa ttatttttgt atatctattc
    14821 ataaaatacc tccatatgcg attaaattta aaaaatatgt tataataatt gtttgtatta
    14881 acttaataat tataacaagg agtaataata atgactagta taaaaaataa agatatttta
    14941 aattgtatag attattccat taaaaataag ctctttgaca aactaaatga tgtatataag
    15001 tccttgccag taggaaattg ttctggatgt ggaaattgtt gtatggagtc agtaggtata
    15061 aatttaattg agttcttgaa tatattttgt tatcttgaag atagagttga tttaaaaaaa
    15121 agctgtataa gtaaaatatt agattattat tttgaggagt atacaagaaa gaacccatgt
    15181 ccatttaaag atactaataa tagatgttta atatatgaag ttagaccatt aaattgtagg
    15241 atgtttggac attggaaaaa agaggattat aataaaaatt tagataatgt tattgagaaa
    15301 aataataact atagagagtt aatgaaaaag caatatggat ttgaaataaa tgatgaagta
    15361 gtaaattaca ggatagatta ttgtgataga tttattccaa ataaagatta tttaagtaaa
    15421 tctaagagat taagcttttt tgatgagctt atgatattag actcaaatat ttattcaagt
    15481 ggaaatatag atatagactt tagggataga ggtatagttg aatattttat agagtcatta
    15541 ctttatagaa atttagcata taatgtgaaa ataaggatat ctaaagaata caaaataaga
    15601 aaaagaacta tagatagaat aaaaaggatt gttcttgtat agttttatat tagtaagtaa
    15661 taaattgtat cataaatatt gtcaaaatga ggtgaagaag gtgaatctta caaaagtaaa
    15721 aggaaatacc ttttttataa aaggtggcac gaatacagga atatatgtat ttgaagataa
    15781 cacagcactt atgatagacc cagggttatg tggttcaaga cctaggagag taaataaagt
    15841 attagatgaa aataatatac aggttagata tattataaat actcatggtc atgatgacca
    15901 ctatggagct tgtaatcaat ttaaagaata ttataaagat gtgtgtataa tgtcatctga
    15961 atacgataaa ttatatatag aaaatccaga attgttttca aagtatattg ttgggggaaa
    16021 gagcaatgta tttatggata atatcttaaa aaataaaatg ttggatgata taaaaataga
    16081 taaatctata gaagctggaa aaattgaatt aaacaatgaa atgtttgata taattgaatt
    16141 tcagggtcat acacctggta gtatagctgt tttgacgaag gataatgtga tttttgttgg
    16201 cgatttattg ataggagatg aaattctatc aaaatacgat ttcttgtttc tatatgatat
    16261 acaagaacaa ataaattcat tagaaaaatt aaaaaatata gattttgagt acttagtttt
    16321 agggcatggc aagcaagtta tatcgaaaga agattcttat aaattaatag aaaaacattt
    16381 gaatgcttta aaaaaatatt tatatcaaat aagagagttg cttgtagaac cgaaatcttt
    16441 agaaatttta ttaaaaaaca ttataaataa caataattta agcaataatt ataaagagta
    16501 tcattttttt aaatcttcgt tagtatctgt cataagttac ttagttgatt tagaagaaat
    16561 agggtatata ttggaaaatg gagaattgct ttactataca aaaacaaaat aaattatgat
    16621 aaaataaaga aggtcataaa aaacaaataa agggtgagca atatggaata cgaaaaatgg
    16681 aatgagatac tagcacctta tgaacatgca gtagaggaat tgaaaataaa atttaaaaat
    16741 ataagaaaag aatatttagc aaaaggtgaa tattctccca tagagtttgt cacaggtaga
    16801 acaaaaaaaa tatcttccat aatatctaag ttaaaaagga taaatgcaaa agatatagaa
    16861 actgaaattg atgatattgc tggtataaga ataatgtgcc agtttgttga ggatatatat
    16921 gctatagttg atttgataaa agtaagaaat gatatgacta taataggaga aaaagattat
    16981 attacaaatt acaaagatag tggttataga agctatcatg ttataataaa ataccctata
    17041 aactccattg cgggttcaaa ggaaattata tgtgaaatac aaataagaac tcttgcaatg
    17101 aatttctggg ctacaataga acattcatta aaatataaat atgaccatta catacctgag
    17161 actcttgctg aaaggcttag aagagcatct gatgctgcct ttcttttaga ccaagaaatg
    17221 agtgaaataa gagaggacat aatgaaagct caggctatgt accaaatgaa gtctatagct
    17281 attagagata ctttaaatag aatacaagag ttatatgact tgggtgatac acataaagct
    17341 atgcagtacc aaagaaaact tgatagaact gaaacagata aaaatataac tgaaattttg
    17401 gaattaaaac aagaaataga tatattatta gagcaatata aggatactaa ggtagatgat
    17461 aaagtagttc agtaaaaact acttgtagaa aatttgctaa atattaatgg ggctgcctct
    17521 taatggaaaa taatttctaa aaagagacag cctatttagt gtaattaaga aaggaatgat
    17581 attatgagtt tgtatgctat aggtgattta catttttcaa catcagtcaa taagccaatg
    17641 aatatctttg gaagtaattg ggatggtcat gaaaagaaaa ttatagataa ttggaaagaa
    17701 gttgttaagg aagaagatat ggtcttagta ctaggagata catcttgggg aattaattta
    17761 tccgaagcta aaaaagattt agatataata agcaaacttc caggtcagaa aatacttata
    17821 aagggaaatc atgattactg gtggacaacc gttacaagtt taaataagct atatgaagac
    17881 atgcgtttta ttcagacaaa tttttatgaa tataaagatt atgcaatttg tggaggtagg
    17941 ggctggatat gtcctaatga tgttaaattt gatgagactg atgaaaaagt ttataaaaga
    18001 gaagaacata gacttagact atcattagaa tctgctcgta aaagtggtca ctcaaaaata
    18061 attgtgatta ctcattatcc tcctacgaat gataaattgg aagaatcttt atttacaaag
    18121 ttatttgaag aatataatgt tgaaaaggtg atttatggtc acttgcatgg taaagaatct
    18181 tttaaaatgg gattgaaggg gattcgaaat ggagtagaat atacattagc atcttgtgac
    18241 tatacagaat ttaatttaat aaaagttcac gattaaaaaa cataaattgg taaatactat
    18301 aagatacatg attaaaaata aatatttgag ggatttcggt tttgagaaag gagacacgac
    18361 ttatgaggtg gatattagtt tttatatttt tatatcttat tcctttagta gtgatattca
    18421 gaaattacaa gaattttaag aggtcttgta tctacagtag tatttatgtg gtattagcta
    18481 caacaataat gataagtaat atttatatga gtggacttaa taaaataaaa gagtctatgt
    18541 attatcacaa ttatatttct gatgagacat atgatgaaaa gtatgtatca aattttaagg
    18601 aaaaagaaag tactgattta aaacaggata ataaaaagaa tgaagaaata tcaaataata
    18661 aaaatttaaa caacaaacaa gatagaaata tacaaaataa agaaagtaat gaagaaggaa
    18721 gtaatgatat aaaagaaaag aataattctg atagtaatgt ttcagtgaat aataaatcag
    18781 aaaaacaaga agataataca aaagaaaata ataaatatac aagtaaagaa aaaaatagtg
    18841 aagatataaa aaaaatagat atggaaagta taggaatttt taagaaagaa atttataata
    18901 tagaaagagc agctttagtt ccaatgagag aatgtatacc ttacactaag gatatagcaa
    18961 aaaatataac taaattatct agtataaaaa aagatgttac aaatgccaga aacaagtgta
    19021 aagaagttgt aaaagaatat gaaaatatgc agattccaga actttctaag gagaagtata
    19081 cagaaacatt gagtaatgct agagatgacg taaagaaagc atatgagctt agagaaaaat
    19141 ctatggaaag tgctttgaaa ttaataaata ctaaaagccc taaatatata gggaagataa
    19201 cagaatatct tgatttatca gataagcaga tagaaagttt taaaaataga ttgaacgatt
    19261 taaaagaaga aataaataat tcagaaacaa ataatattaa ttaataagat ttaaagatat
    19321 aagttaaaag ggagattatg atgaataaac agagtatagg aaaactaaat atgtttttta
    19381 tggggattac tcaaagaaac cttgataata gggatttttt tattgagtta gaggtgattt
    19441 ttaaatctgg aagaaaagag tttaaaggaa ttgcaataga ggaagataat aaatataaat
    19501 ttaattttaa aggaaaatca gatttatata cgtttaatga gttgcttaag aatatttcta
    19561 aagaggcaga aaattatgat ggattagtat ttaaatatgt tgagcgagga actattgttg
    19621 taattgaagg agacaataag aaggtaaatg taaaatattt agataataag gaagaagtac
    19681 ctaaaataga tgaatttacg gcatctcaaa taaaaaacag agattattat gtaaaagtag
    19741 gacaagcaaa tgcactttta aaagaaattg gagtacttac aaaagatggt aagataaaaa
    19801 atgataaaat aagaaaatac aatcagatag accattttgt tgaattgata gatagtattt
    19861 taaaagaaat aaaggataaa gactgtataa ctatcttgga ttgtgcttgt ggaaaatctt
    19921 acttaagctt tgtcttaaat ttttatataa aagaagtatt gaagaaaaac tgttatttta
    19981 taggaataga ttattcagat gtagttatag aagcatcaaa aaatatggct aaaaacttgg
    20041 gttataaaaa tatgtcattt ataaaagagg atttgactaa ttatacacct aatagggatg
    20101 ttgaccttgt aataagtcta catgcttgtg atacagctac agatatggca ataggtctag
    20161 gaattagagc aaaatctgaa gctatagtag tagtaccatg ttgtcataag gagttattag
    20221 ggcaatatag atatgaagct atggaaccaa tattaaaaca tggtgtattt aaagctagat
    20281 ttgcagattt aataactgat ggtttaagaa cattgttatt ggagggcaat ggatatgata
    20341 cttctgtagt agaatacata tctccacttg atacaccaaa aaatttaatg ataagagcga
    20401 taaaaactaa aacgaataat gacaaagcat taaaagaata taaagaactg aaatcacaat
    20461 ttggagtgga accaacctta gaaaaactta tatattagtt aaaaatgtaa aatttacgaa
    20521 aagcttgctt aaatatgcaa gctttttggt attatatagg tatagtcata tccagagttt
    20581 tgatttataa ttaaagctgt gctataatat ttgattaatc aaatcaataa atgttacatg
    20641 gaggtaaaaa tgaacaaatt gaatatatgt attgatatag atggaacaat aacaagccct
    20701 tatcattttc ttccatacct aaatgatatc tttaataaaa atataactga agcagagtgt
    20761 ataacacata attgggaaga attgtatggt tcgggaataa gagatgtgta tgcggagttt
    20821 aataataagt atatacactc atatgatgaa gcaaagattg tagagggtgc tacagaggta
    20881 attagcacat tatctaaagg acataattta tcttttgtaa cggctagaca tgagtgttta
    20941 actgatgtaa ctaaaaattg gctatcgaga caaggatttt cagatataga tgtttactta
    21001 ttaggaagtg attataaagt tgaaaaggca agagaactga gttgtgacat atttatagaa
    21061 gataatcctt taaactcagt gcaactagct gatgatggtg ttaaagtaat acttctggat
    21121 actaactata ataaagatgt taaacatcaa aatatagtta gagttagtaa ttggatagat
    21181 attgaaaaga ttataaaaca aggtatttaa agatttgtag attgaaaggg ggagcatatg
    21241 atagatttaa aaattgaaaa tagaaaagga aaaatccatg taaagagcag tgaaataaaa
    21301 gacattttag aattacgtcc agacttcgaa tatgtacaag atatatcaaa tactataaat
    21361 caagaaaata tattggtatt tgattgccag ttgacaggaa attcttttga tatggatgat
    21421 ttagatatag aagaaatatt ggaagagtta ggtgaagaaa tagatgagtc ttatttcagt
    21481 gttttatttg aagatgtaag agcgtattta aaagatgcta ctgatgagat agaagcagac
    21541 ttacaggata attacttatt tgataatttg agatgttatt ttgatatata caatataaat
    21601 caagaattta cagattttaa gtttgtattt ttagtttctt ttaaagatat aaaaatttct
    21661 tcattagcaa acctagctaa gattgtttct aagagacagc ttataggtgc atctaagttt
    21721 tatagttaaa atgaaatatt attataataa aagctagtaa ccttgtaaaa atataggtta
    21781 ctagtttttt atttattcat tttcacaaaa tttcagaaaa ctatttacat tctaaaaaaa
    21841 gtaatgtata ttaaacataa tgtacattat aatggataaa aagtttctat taaattacta
    21901 agtttattta ataatcaaaa taatttatct tatattagta tgtattaggg ggtatttatg
    21961 caaagtttaa atgaaataat tgctgaaaat ttaaaaaaga taaggaaaga aaaacatctt
    22021 agtttagaca agattgcaca acttagtggt gtaagtaaga gtatgctatc tcaaattgaa
    22081 aagtgtgagg taaatcctac tatatctact ttgaagaaaa taactaatgg gctaaaaatt
    22141 tcatttacat cattgatgga gaggcaggaa agtgacatag aattgattca aaaaagtgat
    22201 atagaccatt ttattgaaga taatggaaag tatataagtt atccaatatt tccatttgac
    22261 tccaaaagaa gatttgagat atttatgatt gaaatagaag aaggtggaaa tttagattca
    22321 aatgctgaac ttccaggaac acaagagttt ataactgttt ttagtggcga agtaaccatt
    22381 aaaataaatg gagaagatta cattgtatct agtggaaatt ctataagatt taaagctgat
    22441 gtatcacata tatatagaaa ctcaggcaaa ggtatagcta aaatgagtat ggtagtatat
    22501 tatatataaa taatttaatg gaattagagt gatatttaga tgattttagg tatgaagata
    22561 tattatattt aagttatagg agtgtgattt atggatgaat aataaaaaag cagttttttt
    22621 cttttgcagt gatttaaaga aagacccagt agccagtaat gtgataaaat gttgtgaaga
    22681 aataatggat ttaaatcaaa ctgatattgt tattgatgat aattttgtat tagagtttaa
    22741 ggataaaaac aataacttat ttcattttgt aaaaacaaaa gatgtaataa gtcataatta
    22801 taaatattat cttccaattt taaatagaca ttttagagat tatgattttg caggagttgt
    22861 aaattggcat gagggagaaa atgctccaga acatatccta acagctcata caattggaga
    22921 tgttccaaca ggagaatttg gaaattcaaa tccaagatac tttaaaaatc taatcaatgc
    22981 tattgaggat ataaaaaatg aaaatctact tgatgaattt actacattaa cagaagcaac
    23041 tcactggtct ggaacaacat atggtgaaga atctaaatta attacagaat attctgtacc
    23101 tatgttggat attgagatag gaagtagtag tgatagtttt aataatagta tagcaataca
    23161 ggtattggca aaatcattaa ttagagtatt tgattgtgat gaacctttga agactttgtt
    23221 atgtgttgga ggggtacatt ttgagaaatc tttctcagat attataaaaa ataaagaata
    23281 taacatatca attggacatg tattgccaaa tcaatggatt gtaagtggaa tgtatgatga
    23341 tgaatctgga tttgagaaac tagaaaagtg tataaatagc attgaaggag gaattgattg
    23401 tatagttttt catgataaat taaaaggtac ctataaagag caatgccgta aactaggtga
    23461 aaaacttaat gtacctgtat ttaaacataa aatattaaaa aatccaaaag acttacctat
    23521 atggtagtct tatacagtta atataacaat aagtcggatt aatttttaag cgaggtcaaa
    23581 aatgatatta tcttattttg cattattaat tagagtagtg ctattagctt ttgaaagagt
    23641 agttgtaaga ttattaggtg atgaagaagg agatatttat aaaaatatag cttcatcatt
    23701 tctatttttc tttatagggg gagtatgttt acttccattt agcataatgg atagagttag
    23761 tgattggtcg tttttaatac catgttatat aagtagttta gtctattcaa ttggttcagt
    23821 agcgtatgta acgtctttag caactgggga agtttcactt gttactccaa taaatagttt
    23881 aaattcattg tttttactgc ttttatcagt tatattttta ggtgaaggtt taagtttagc
    23941 aaaagttgca ggaatcataa ttatgattgg tggagtgttt atattaaaaa aagtaagttc
    24001 ttttttaaag aatgtctctg taatttttaa caatttacca tgtagattaa tgtttttata
    24061 catatttttg cagtcagtag gaagagtatt ggataaggct ttttttgtac aagtatctcc
    24121 tatattatat tcaactatac tgtatttttt cgtaggttta aatttattta tctttttagc
    24181 atttaaaaag aaacaaaaaa taatttgtga gatttttact aataaaaagg ggctttcaat
    24241 tttaagtggt gctattaatg gatattctta tttagcatta ttaattgctt taaataatct
    24301 agaacttagt atagcagagc cattttctca agtatctatg ataataactt taatacttgc
    24361 tcattttata ttcaaagaaa atataaaaga aaaaatacca ggttcaattt tgatactaat
    24421 tggtgggtgg ctactgttac tttaattgac ataaaaaatg aattataaag gtgctattag
    24481 ttagtatcag aaaatttaaa aaagtattga caattctttt aggcaaatgt taatataata
    24541 atacaaaata gtataaatag aagacatgtt actggtaatg caggcaggat cgaatatatt
    24601 gctttgattt ttcaaggtgt ttatataggt tctgcctttt ttgcatcttg aatggccatt
    24661 cataaaaaat tatggagaag gagattacaa gatgaattat aaaaaaactg ccattgacat
    24721 tattgaacaa gttggtggtt caaaaaatat tttagcttta gaacactgtt caactagact
    24781 acgctttaag ttgattgata gaagtaaagt caatcaagat gctttaaaaa aagtacaagg
    24841 tgttatgagt atagttaatg gggcacaatt tcaagttgtc attggaaata gtgttgttga
    24901 tgtgtatgat gaaattttaa aagtttgccc tttagataca aatgatagta ctgtaaaaac
    24961 tgaagaaaaa ataaacttag gaacaagatt tatagaattt attattaata tttttcaacc
    25021 actgattttt gctattgcag gagctggtat tttaaaatca ctgttgatgt tattagcaat
    25081 gtttaatatt ttaaaatcag attctactat atatacatta ttagttgcta tttcagatgc
    25141 aacattctat ttcctacctc ttatggtagc tgtaactact gccaatgtat tgaaatgtaa
    25201 tcgtttagta gctattgccg ctgtaggata cttattgctt ccagcaacaa caactgcttt
    25261 aagtgaaggt gttgaactat ttggatttac aattccaaac attgcctata atgcacaggt
    25321 tttcccggct atcttatgtg tgtctttctt ggcaacaatg gagaaaattt ttaacaagta
    25381 ttcaccaaaa ccaattcgaa ctttctttgt tcctatgatg tctttggcaa ttactgttcc
    25441 aattactcta actattttag gtcctttagg atataacgtt ggtacaattt ttacaaccat
    25501 tattttattc ttatataaca aaatgggatt cttagttgta ggattacttg ctgcttttct
    25561 tccatttatg gttgcaacag gaatgcacaa agcactaata ccttattcaa ttaatacaat
    25621 tggaaagttg ggttatgaag ctttatatat gacatcttca ttagcccaca atatttctga
    25681 aagtggtgct tgttttgcag tagccttgcg aacaaaagat gaaacattaa aacaaacagc
    25741 attatctgca ggaatttctg ccttgatggg aattacagag ccagctttat atggtattac
    25801 cttacaacat aaaagagcaa tgttaggtgt tgtattctca agtgcaattt ctggtacctt
    25861 tttaggatta tttgcagtaa aaggatttgt aattgttgga ccagggcttg ccagtatgag
    25921 tatgtttatt gacccaaaca atggaaacaa tttgattttt gcgagtattg gttttgttat
    25981 tgcaatatta ggctcgttta ttattacact gattatttgg aaggaagata caaatacagt
    26041 agagtcatca attggagatg ttgttgaaga agagctagaa gaagaaaaag aagtaaatat
    26101 cttagtaact ccagtagaag gaaaagtaat agatttaagt aaggttaatg atgaactatt
    26161 tgcaagtaag actttgggag atggagttgc aattatacca accaatggaa atttatacgc
    26221 accttgtgat tcagaagttg tgatgttatt tgaaacaaaa catgcaattg gtttaagaac
    26281 aaaaaatggg gctgagattt tgattcatat tggaataaat actgtaagta tgaatggaga
    26341 tggatttaaa acatttgtta agacaggcga taatgtaaaa gaaggagatt tattaattca
    26401 atttgattta gacaaaatct cacatgctaa tttagattca actgttatga ttgtaaacaa
    26461 taatggagca gattatgctt ataaagtgtt aaaccagtct tatggcaatg taaaaaaagg
    26521 aagtatcttg tttgatgtaa agggaggaat ataaatatga gaaaatttcc agaaggattt
    26581 ctttggggag gagctacagc tgctaatcaa tttgaaggtg gttggaattt aggtgggaaa
    26641 gggtggtctg tatcagatgt agcaaaggca cactttgatg cagatgtgaa agattataaa
    26701 tctaataatg aaatcacaac aaaagatata gaagaaggat tagcacatcc tgaagatgaa
    26761 gtaaattatc caaaacgtca tggtagtgat ttttatcatc actataaaga agatattgct
    26821 ctaatggcag aaatgggatt taagacatat cgaatgagta ttgcttggtc tcgtattttt
    26881 ccaaatggag atgataaaga gccaaatgaa gaaggtttac agttttatga tgatgtattt
    26941 gatgagttga taagttatgg aattgaacca cttgttacta tgtcacatta tgaaccacca
    27001 ttaaatatcg ttttaaacta tgatggatgg tattcacgac aagttatcaa tatgtttgtt
    27061 aggtatgtag aaactatctg tgaaagatat aaaaacaaag tgaaatattg gcttacattt
    27121 aatgaagttg attctatgat tcgtcatccg tacactactg gtggtttagt tcgagaccgt
    27181 tttaaggata aaaactttga ggaagttatt tttcaagcta tgcatcatca atttgtagct
    27241 agtgccattg caacaaaaat ttgccatgag attattccaa attcaaaagt agggtgtatg
    27301 ttaacaaaat taacgtatta tccatataca tgtagaccag aagatgtttt ggcaactcaa
    27361 caaaaaatga gaagtattta tgcatatagt gatacgcagg tttttggtga gtatccagta
    27421 tatttactgt cgtattttaa aaataataac attcaaatag taaaagaaga acatgatgat
    27481 gaaattatga aaaagtatcc agtagatttt atttcattct cttactatat gtcttcttgt
    27541 gaggcagctg atacaactgg attagatatt acacctggga atacgttatt agctgtaaaa
    27601 aatccttatt tagaaatgag tgaatggggt tggcaaattg attcaatagg tctacgtata
    27661 tctttgattg aattatatga tagatacaga aagcctttat ttatagttga aaatggattg
    27721 ggagctaaag atattttaac tgaagataaa aaagttcatg accaatatag aattaattat
    27781 ttaaaagaac actttaaatg tatgttagat gcgattatcg aagatggcgt tgaactttgg
    27841 ggatatactt catggggttg tattgatttg gtcagtgaat caacgaaaca aatgtcaaaa
    27901 cgttatggat atatttatgt ggatgctgat gactatggaa aggggacata taatcgatat
    27961 aagaaagata gtttttattg gtataaaaag gtaattgaaa ataatagtat agattttaaa
    28021 tgaccaatag aaggtgagca aataaaatga actacataat aaagaaagtt ttgaactcga
    28081 gcgttgtttt agttcatgat gcaaagtata atgaatttat tttgctaggg aaaggaattg
    28141 gatatggtaa aaaaacagga gaatatattt gtggttccaa tgataatcaa atgtttgtcc
    28201 ctgttgaaaa tacaaaatca aaacaatttt tagaattaat ggatgatatt cctgtcgaca
    28261 ttttaaagat aacacaggaa ataatagtag aggcagaaaa gttattgaac tcatctttta
    28321 ataaaaactt atacttaata ttagcagacc attttaactt tgcaattgaa aggatgcgta
    28381 aaggaataaa aattacaaat cgagtatttt gggaaataaa aaattattat ccaaatgagt
    28441 ttaaggttgg aatggttgca atttatatgg tagaggagag attgggcatt caattaccag
    28501 aggaagaagc agctaacatt gctttccatt ttgcaaatgc tatggcttca gatggaaatt
    28561 catatgatac gattaagtat gccaaagtaa ttggagaaat aattaatatt tttgtttttt
    28621 ctttgaatag aaccttagat aaaaagagta tgcattatat gagatttata acacatataa
    28681 aattttttgt ggaaagattt ttttcagata gtatgttaag cagtggtgat gatttattat
    28741 ttaatcaaat gaaaaggtca aatccaaaag aaatgttaat tgcattaaaa gttagagatt
    28801 ttttagaaaa gaaatatgga aaaaagttaa caaatgagga aatagctttc ttggttgtac
    28861 atatcgcacg agttaagcaa tagagtgaat tactaatatt atcttatacg tctacatggt
    28921 agaaaattga aagaaggtaa agtatataaa gaaactgaga catattattt tgttgttcat
    28981 tatgtttatt ttatgagtgt atcctgttaa aaatttaagt taatatttat aattaaaata
    29041 tacaaatagt taaaataagt ttatgtggtt atgaatttat tttaactatt tttttgttta
    29101 atggaaaata tgttttataa ctagaatttt atttttaatt aattata
//
LOCUS       DAMJZE010000030        29034 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJZE010000030 DAMJZE010000000
VERSION     DAMJZE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.030"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            21..722
                     /locus_tag="PC541_001229"
     CDS             21..722
                     /locus_tag="PC541_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2838700.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            946..2247
                     /locus_tag="PC541_001230"
     CDS             946..2247
                     /locus_tag="PC541_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2838701.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            2384..3478
                     /locus_tag="PC541_001231"
     CDS             2384..3478
                     /locus_tag="PC541_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2838702.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            3480..4613
                     /locus_tag="PC541_001232"
     CDS             3480..4613
                     /locus_tag="PC541_001232"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2838703.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            4631..5740
                     /locus_tag="PC541_001233"
     CDS             4631..5740
                     /locus_tag="PC541_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2838704.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            5805..6545
                     /locus_tag="PC541_001234"
     CDS             5805..6545
                     /locus_tag="PC541_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2838705.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            6550..7542
                     /locus_tag="PC541_001235"
     CDS             6550..7542
                     /locus_tag="PC541_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2838706.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(7730..8611)
                     /locus_tag="PC541_001236"
     CDS             complement(7730..8611)
                     /locus_tag="PC541_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2838707.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            8884..10272
                     /locus_tag="PC541_001237"
     CDS             8884..10272
                     /locus_tag="PC541_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838708.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPKESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            10300..11253
                     /locus_tag="PC541_001238"
     CDS             10300..11253
                     /locus_tag="PC541_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2838709.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            11313..11537
                     /locus_tag="PC541_001239"
     CDS             11313..11537
                     /locus_tag="PC541_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838710.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            11530..12042
                     /locus_tag="PC541_001240"
     CDS             11530..12042
                     /locus_tag="PC541_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2838711.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            12074..12928
                     /locus_tag="PC541_001241"
     CDS             12074..12928
                     /locus_tag="PC541_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2838712.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            13462..14256
                     /locus_tag="PC541_001242"
     CDS             13462..14256
                     /locus_tag="PC541_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2838713.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            14360..15166
                     /locus_tag="PC541_001243"
     CDS             14360..15166
                     /locus_tag="PC541_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2838714.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            15228..15749
                     /locus_tag="PC541_001244"
     CDS             15228..15749
                     /locus_tag="PC541_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2838715.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            16058..16213
                     /locus_tag="PC541_001245"
     CDS             16058..16213
                     /locus_tag="PC541_001245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838716.1"
                     /translation="MKLKTIKFSNKISIVEVMKESNDNMKTNLNKKVGVNGSGQRNCI
                     NSPDFIK"
     gene            16164..18251
                     /locus_tag="PC541_001246"
     CDS             16164..18251
                     /locus_tag="PC541_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2838717.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            18266..18553
                     /locus_tag="PC541_001247"
     CDS             18266..18553
                     /locus_tag="PC541_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838718.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            18580..19851
                     /locus_tag="PC541_001248"
     CDS             18580..19851
                     /locus_tag="PC541_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2838719.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            19909..20508
                     /locus_tag="PC541_001249"
     CDS             19909..20508
                     /locus_tag="PC541_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2838720.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            20513..21418
                     /gene="dapA"
                     /locus_tag="PC541_001250"
     CDS             20513..21418
                     /gene="dapA"
                     /locus_tag="PC541_001250"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2838721.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            21524..22156
                     /locus_tag="PC541_001251"
     CDS             21524..22156
                     /locus_tag="PC541_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2838722.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            22633..25140
                     /locus_tag="PC541_001252"
     CDS             22633..25140
                     /locus_tag="PC541_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2838723.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            25482..27644
                     /locus_tag="PC541_001253"
     CDS             25482..27644
                     /locus_tag="PC541_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2838724.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            27649..28266
                     /locus_tag="PC541_001254"
     CDS             27649..28266
                     /locus_tag="PC541_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838725.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            28455..28799
                     /locus_tag="PC541_001255"
     CDS             28455..28799
                     /locus_tag="PC541_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2838726.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        11300 a         3143 c         5190 g         9401 t
ORIGIN      
        1 tataatttaa gaggtgaatc atgttaagaa atcaaccatt atatatacaa gttgtagata
       61 tattaaagca aaaaatatta aataacgaat ataaaattga tgaaaatatt ccaacagaaa
      121 gggagttaga gcaagagttt ggagtcagta aaattactat tagaaaagct attgagatat
      181 tagaatcaga aggatatgta gagaaaaaaa gtggaaaagg gacaaaagta ataagtaata
      241 gcctttttaa taagctatca aaagcagagt ctttttcaag tatactttta tctaaaggtc
      301 ataagttatc taaagagact atttcaattg aaaaaataat aaatgataca aattcacaag
      361 tatttagtat atttggagaa aaatgtagca agattactag aatgtattat ttagatagta
      421 agccatatat attttttact cattatattc cttttgatga aaatctaact gttgaaagtt
      481 ttaataatga aagttcttta tatatgtact tatataggag aaatataaat ataactagat
      541 ttgaagatga attttttata gaagaatcag aactagaggt atcaaaggct ttaaatctag
      601 ctaaagacat attattagga agaaaaagaa agacctatga tgagaatgat aatatagttg
      661 agctatcatt tgcaagatat aatacagaag tacaaaacta tgtaattaaa tacgaaatat
      721 aataagaatc ctgagtttat tgaaatgata atatgctcag gatttttgtt tagttgtaaa
      781 aataaaaaat attataacat tataatgttt acaaccaaac attataatgt tataatattt
      841 ttaataaaca agtgatatta tcaattacat aaaaattagt ttatgttatg aaatcgttat
      901 tgcattataa ataaaggata atatcaataa tacaggggga taactatgga aaaattcgca
      961 agttttttag agcgcaagat tatgcctgta gcagcgaaga taagtaatca gagacatatg
     1021 agagctgtaa gagcaggtat aatagcaaca ttaccactaa caatagtggg gtcatttttc
     1081 acaatattat taaacatacc tattgatgct tatatggatt ttatagctcc atttaaagca
     1141 atattagata taccatttag atatactgta ggatttttat ctctatatgc tacttttggg
     1201 atagcatcat cacttgctaa aagctacgat ttagatacaa caggtgttgg tatgcttgca
     1261 gttatggcat ttttagtttc tacaatagtt ccaactcaag tagttgaacc agtaggtgga
     1321 gttatagaag ctggtagatg gatggatatg gcaaaactta attcgccatc attatttgga
     1381 gctatcgtaa catcattaat tacagttgaa atatatcgtt ttatgaagga aaagaatatt
     1441 acaattaaga tgccagcagg agttccagta gaggtttcaa attcatttac tgcattatta
     1501 ccgacagcag taattttaat gttattctgg gtaataagac atattctagg atttgatata
     1561 agtgttgcac taagcgctat gttatcacct ttaaaaggat tcttatcagg aaatagttta
     1621 ggtggtgggt tactgacagt attcctaata actttattct gggtacttgg aatacatggt
     1681 ccagctataa tgggaccagt aataagacct atatgggaca tgtctatagc agaaaatata
     1741 actgcattca cagaaggagt atcagctcat gcaatgccaa acatatttac agaacaattt
     1801 ttacagtggt atgtatggat agggggagca ggggctactc ttgcattagt tgtaatgttt
     1861 atgttttcta agtcacaata tctaaaaagt ttaggtagat taggattttt accagggtta
     1921 ttcaacataa atgaacctat aatatttgga gcaccaatag ttatgaatcc aatactaggg
     1981 ataccattta taattggacc tataataatg acaattctat catatgttct tactattaca
     2041 ggggttatac ctatgatggt agcaagatta ccatttgcaa tgccagcacc aatagcagca
     2101 gtaatgagta ctaactggag tatactagca ggtatcttag tattaatcaa ctttgtgata
     2161 tcatttgctg tttattatcc attcttcaaa gtttttgaaa aacaacaatt acaaagagaa
     2221 caagaagaaa tgagtgaaaa tttataaaaa tacatagata tagaggtttt gtactatata
     2281 aaatatagaa aaagcttata agttattaag ggtgaaggta ggaggaatga cctttgcctt
     2341 taattttata tattttaaat aatgtaattt gaggaggaga aagatggaaa gaagattagg
     2401 aatatcagta tatccagagc atagtaacat ggaagatgat aaggcatata taaaaatggc
     2461 ttcaaaatac ggatttaaaa gaatatttat gtgcatgctt tcaattacaa gacctaagga
     2521 agaagttaaa aaacatttta aagagattat aacctatgca agagaattag gatttgaagt
     2581 aatccttgat atagctccaa acatatttga tatattacaa atatcttatg atgatttaac
     2641 attttttagt gagttaaatg ctagtggaat aaggcttgac tatggatatg atggaagtaa
     2701 agaagctatg atttctttca atccatatga tttaaaagtg gaactaaata tgagtaatga
     2761 tgtagcatac ttggataata taattactta caagccaaat gtaaataata tatatggttg
     2821 tcacaatttt tatcctcaaa ggggtacagg cttgccatat gatttttttg taagatgttc
     2881 aaatagattt aaaaagtatg gaataagaac tgctgctttt gttaattcaa aatacgcaac
     2941 aatagggcct tggaatataa atgatgggct gtgtacgctt gaaatgcata gaaacttaga
     3001 tattgctaca gcaaccaagc acttattcgc aacaggattg attgatgatg taattgtagg
     3061 gaatgcatat gcaagtgaag atgaattaaa aagaatgtct gaagtaaata aatatcaagt
     3121 tgtatttaat gtagtaactg aagaaaaaac ttctgaagta gaaaagacaa taatggcaaa
     3181 tgagcaacac tttagaagag gggatataac caaccaagta gcaaggtcta cagaagtaag
     3241 aaaaaaatat gctaaagaaa atttccctaa gcatgataat acgaccatct ttaaaagagg
     3301 agatgtagtg attggtaatg atgactttgg aaagtataaa ggggaattac agttgatact
     3361 tcaagattgt gcagatgaaa gaaaaaataa ggtgggtaga ataaaggaag acgaaataat
     3421 acttttagac tttattgaac cttggagtaa atttaaactt attttagagg gagagtaaag
     3481 tgaagataga catattaata aaatgtggaa aaacagtaga taaaagtata attgatatag
     3541 ctatattaga cgataaaata atagaagtaa aaaatcagat agatgattct aagtatcagg
     3601 caaatcaaat tataaaccta gatggagaaa aatttattag tgctggttgg atagatatac
     3661 atacgcactg ctacgaaaga ctagaccttt ataaggatta tccagatgag gttggaataa
     3721 aaagcggtgt tactactgta gtagatgcag gaacaacagg agctttagat ataggtaaat
     3781 tttatgaaga tactaaaaaa tataaaacaa atgtatatgc tttaataaat attgctaagc
     3841 agggaataac atctcaagat gaactttcaa gtatgatgaa tattgatgaa tatgaactta
     3901 gaagagcagt taaaaaatac aaagattttg ttgttggaat caaagctaga atgagtaaaa
     3961 gtgttgtaat ttcaaatgat gttgaacctt taaaagtagc taaaagaata aaaaatgaac
     4021 ttaatctacc tatgatggtt cactttggga gttctcctcc aacgatagag gatatatttg
     4081 actatatgga aaaaggagat atattaaccc atatatataa tggaaagcca aatggaatac
     4141 ttagaggcaa tgaggttaaa aaagaaatca tagaagctag agaaagaggt ataatccttg
     4201 atgtaggtca tggaaccgaa agttttagta tggatattgc aatgaaatct aaggatgcag
     4261 gaatttttcc agacactata agtacagata tatatattaa aaatagaata aatggtccag
     4321 tgtataatct atctacaaca atggaaaagt ttatatatat gggctattca cttgaagata
     4381 taatagataa agttactaaa aatgcagcag atgcaataag tttaaaaaat aaaggtttaa
     4441 taaaagaagg atatgatgct gatttaacta tatttgatgt ggttaatgaa gaaaaagaac
     4501 ttcaagattc tttgaataag tcagtaatca caagtacgtc tataaagcca agagcagtag
     4561 tagtcaatgg agaatattta aatataggta agtctatagg agagaatgag tagaagggag
     4621 aaactattgt atgagtagta tatttgaaaa atactctgta agacgtgtta taaatgcttc
     4681 aggcaaaatg actatactag gagtatcaac agtagataag gaagttattg aagcaatagg
     4741 tgaagggtgt aaaaatttct ttataataga agaacttata aataaaacag gtgaatatat
     4801 agcaaattta ttagggtgtg aaagtgcact aatagtttct agtgcatctg ctgggattgc
     4861 acagagtgtg ggagcaatga tagcaaaaga tgatatgagt ctagtttaca atgtaaataa
     4921 tcctaagaaa caagttaaaa gggaaataat aattccaaag ggacataatg ttgattatgg
     4981 agttccagta gaagtaatga taagacttgg aggaggagaa gttgttgagg ccggttatgc
     5041 taatatgtgt aacaaggaac atataataag agagataaat gaaaatacag cagcaattat
     5101 gtatattaaa tctcatcatt gtgttcaaaa gagtatgcta agtgtattag atgcagtaca
     5161 agttgctaaa gaatataatt tacctttgat agtagatgca gcagcagaag aagatttaaa
     5221 tgtatattat gaattgggag cagacctagt gatatacagt ggtgctaagg ctatagaggg
     5281 tcctagctca gggttagtaa taggtaagaa aaaatacata gataatgtaa agcttcaatc
     5341 aaagggtata ggaagagcta tgaaaatagg aaaagaaaat attgtaggtc taacatgtgc
     5401 aattgagaga tatataagta atgataaagt aactttaaaa gaaatggagg ataagctaaa
     5461 tccatttata gacaagataa atactataaa gggagtgagt gctagtataa ctagggattc
     5521 agcaggtaga gaaatacttc gtggagaaat tgactttaat gaagatataa taaataagtc
     5581 aacacaacaa attataaatg aacttagaag tggagagata gctatataca ctagagacta
     5641 taaggctaat gaaggcaaga ttgagattga tataagaagt gtaactggaa gtgatttaga
     5701 tactatttat aataggataa aaaatatagt agaaagttag agtgtagagt aagtgacatt
     5761 ataatgattt aataaattaa attttaccaa tggaggggat aaatatgaat tgtataccac
     5821 aatattataa aaatagagtt tgtttaaatg ttcttgcagg aagcataaaa aatgctaaag
     5881 aagtatatga tgcttgtgac gggcatgtat tgataggaat actatcaaag aattatagca
     5941 ctgtagaagc agctattgaa gatatgaaaa aatactcaaa ggaaatagat aatgcaatat
     6001 ctgtggggct tggagctgga gacccaaatc agtcaaatat ggtatctcag atttctaaag
     6061 aacttcagcc acaacatata aatcaagtgt ttacaggtgt ggctacgtct agagcattgc
     6121 ttggtcaaaa tgatagtata ataaatggat tagtttctcc aactggtaaa ccaggatttg
     6181 taaaaatatc cacaggtcca ttaagttcaa aagaaaaaga tggtatagtt cctatagaaa
     6241 cagcaatagc tatgatgaaa gatatgggag gaagctcaat taagtttttt ccaatgggag
     6301 ggcttaaaac aaaggatgaa tatatagaag ttgcaaaagc ttgtgcaaag cataattttt
     6361 atttagaacc aacagggggt atagacttag ataacttcaa agagatagtt caaattgcac
     6421 ttgatgctgg tgtagaaaaa gttatacctc atgtttatac atcaataatt gataaagaaa
     6481 caggtgaaac gaaagtggaa gatataggag tactttataa aataatgaaa gaattattgg
     6541 gatagattta tgaatatatt agcttttggt gaaataatga tgagactttc agtacctgac
     6601 tacaagttct taacacaaac caatgaacta aattatataa ctacaggcac gggattaaat
     6661 atattatctg gacttaaaaa ctttggatac aatacctata tgttgacaaa gttgcctaat
     6721 aataatgtag gtaaagcatc aagtgcaaat ataagaaagc ttggagtaaa agatgacttt
     6781 atcacgtatg gagggaatca tataggagtg tattttcttg aaaatgggta tggagaaaga
     6841 ccttctgaag tgacttattt gaataggtta aatagttcat tttgtgagag taaattacaa
     6901 gattatgata ttgataaatg tttagaagaa atggatatag tccatatttg tggtattgct
     6961 atgcaactta cagattcagt taaagagatt gctttagaac tagctagaaa atcttatgaa
     7021 aaggggataa aagtattctt tgattttaat tttagacaat cattaaatcc agaaaggact
     7081 tatgatgatt tagtaaatga ttataagaaa atactaccat attgtcatgg gctgtttgct
     7141 agttatagag atataaataa gatacttaat atagatagtc taaatataaa atctagttca
     7201 gaattgatta aaaaagagta tgatatagat attgtagctg gaactatgag aagatttgat
     7261 gatatgaata atagatttat aaagggatat atatcaaatg atgatggtta ttttgaaagt
     7321 agggagtata agctagaaat atatgacagg gttggtgccg gagatggata tgtagcagga
     7381 attatatatt catatttgga aaatatgaag agtgaagata tagttgagtt tggggttgct
     7441 agttcggtgt tagctcatac aacttatggg gataattctt tagtaagcac aggtcatgta
     7501 cttagactga tgaataatga aaaaatagat atattgagat aatacatttg tatataataa
     7561 aatttttatc ttttttagac acatttttga cttgcttata tttgttaaac agagttattt
     7621 ttaatgatta taaatattaa attaaaagtt aaataataaa atagtagaaa atagcattaa
     7681 ctatagaaaa aatggttaat gctattttta tcgttaatat tgattaattt tatattatag
     7741 aaatgaactt ttctaatgct ggatttgtat tttttttatt ccatacaact acaacttcaa
     7801 ttctattttc tacttctttt ataggtacta ttgcaatatc acgttctatc attgtatctg
     7861 taacacaaaa tgaaggtaaa atggatattc ccatgttaga agataccaat gattcaatag
     7921 tattaagatt atttgaacgt tgtaccacat taggtacaaa ccctgcttca cgatatctat
     7981 tctgtatcat tgacttttgt ccatactcat cccctgtttc ctttacaaaa ataaatttat
     8041 catgttgtaa ttcaattaaa tcgatagaat cctcattgta aagtggatga ccaggaggta
     8101 aaagagctac caaagaatat ctttctacag ttttatattc aaaatctttt aatggttgat
     8161 tttctggatt aataacaaaa gcgaggtcca tagaattatt ttttactaag tttaaaagtt
     8221 cggttatttc attagtacaa atatccatag atatattagg gtatgtatta gaaaattctt
     8281 ttaaatattg ttgaagtttg tttttttcat atcctattaa ggttccaatg ctcaaagttc
     8341 caaaaaaacc atgtgcaaat tgttgtgttt ttgcaatagc atcattgata ttttttatta
     8401 tagtacgtgc ctcagataaa aaaacttttc cagctggtgt tagttcaaca tgacgtttac
     8461 ttcttgtaaa tagagttaca tttatttgtt cttctaaagc ttttatttgc tgtgtaacag
     8521 cagtttgaga aatataaaat ttttctgctg ttttggtaaa acttagattt tctgctacag
     8581 atataaaata ttctaattgt ttagtattca aataatcacc cctatttaaa cttgattgtt
     8641 tgtaaacctt atttatctat aattaaagct tattatagta taagaaaatt tacttttaaa
     8701 gaatattaga aaatatttgt ataaaaatat atgttttaag tatatcttta cattagattt
     8761 tataaattta ttaataagaa taacttatat attccaatga aaacgatatt ggatttaagt
     8821 aagtttgtct cataaaatga aagtatatca attgataata ctttaaataa gaagggagaa
     8881 aaaatggaag ataaatttta tgcaaaaggc aacggaaata acggatatat taaaaatctt
     8941 gaagtttgtt cctttaataa cttagatgga acttgtggaa tgtttcaaat ggctctgtac
     9001 aaaagagatg aaaaatacta tttatatgga tgctgttttg gaggaaataa aaaaaatgga
     9061 gtaatgatta gcgatattac agacccttat aatccacaat ttataaaaca ttttcaaatg
     9121 ttagacccta aagagtatcc tacaacaaca actcccaaaa ttcaaatagc agatgattta
     9181 atgatagtag caatgagttg tggaagtgga ccaggagcac ttgttgacca agctaaatta
     9241 gcaaatatta agtgtgaagc aggaattaga atatatagtt taaaagaaga ccctttaaat
     9301 cctaagtttt taggatattg ggattgtggc ttaaagcatg taatgggtgt tcatagattc
     9361 atgtacaatg gtggaagata tgtacattta tcaagtgatt gtgttggctt tgaaggtctg
     9421 atttataggg tcatagatat aataaatcct actaatccag tggaaatagg taaatggtgg
     9481 agaccagacc aatatgcaaa tggatatcca aatagaactt ttgatgcagg agcacctcat
     9541 tgcccagaat ttatggataa aggatggctt catggacctc catttgtaag agacggaaaa
     9601 gcatattgtg gttatggagg agctggttta gttgtattag atgttgaaga tttaacaaga
     9661 ccaagatgct taggtgaatt gccatttatg cctgcatttt ctagtagact tgcaggtgca
     9721 agaactcata cagcattacc attgccagga agagatttag tcgttgttca aaatgaggga
     9781 gaaagattcc agttctttaa accagataac attacagatg ttcaagctat gaataatata
     9841 catatggttg atgttagtga cccaacaaaa ccaacattaa ttgctcaatt tccatatcct
     9901 gaagttccaa aagatttccc ttatcctaac tttaatgttg cgggattagg aaaaccaggg
     9961 ccatttggcc cacataatct tcatgaacca atggataata agccatggtt agagcaaaga
    10021 ggagatagag tatattgctg ttatttccat gcagggctaa gggtttatga tgtatcagac
    10081 ccatattata tcaaagagct agcatatttt ataccaccaa atccaaataa aacaccaaaa
    10141 gaatcttatt tcccaggatt cccaggacca cgcttggcag taacagaaga tcttatcgtt
    10201 gatgatagag gctacatcat catagatgct ttagatgatg gattctatat attaaaaatg
    10261 aaagaagatt aaataaatac taaagaggga gagttgaaaa tgaaaatact aggtatttct
    10321 tttggaacaa aaaatggaaa taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa
    10381 gaagcagggg ctgaagtaga atttattcaa atgtcatcta ttaacattaa acattgtact
    10441 ggatgttgtg cgtgtgttaa aactttatta tctgggaaag gtagtatgtg tgtattaaaa
    10501 gatgattttg aatggttgct tgacaaaatg aaagatgcag atggtatagt agtttcagac
    10561 cctatttttg aagaaggggc atcaggatta ttccatacta taatggaccg ttttggaccc
    10621 agagcagata gaggaaataa cattatagga acaaaagttg cagaagctat aggaggaaaa
    10681 attccagatt caagaatgct taatgataaa gtaatatcat ttatgggaat aggaggttct
    10741 gactggggta caagagttca atgtgaacat gctatgctag ctctcatacc aatgtggaaa
    10801 gttatagata atgcttggtt cccatgggca aaggaattag taatggatga tacaaggctt
    10861 gttcaagttc atcaaattgg tttaaatata gttgaagcag caaaagattt tgaaaaagct
    10921 tcatatcaag gagaagaagg tgtatgtcca cattgccaca atagattatt ctaccttgag
    10981 ccaggtacta aaaaggcaat ttgtgctcta tgtggtatag ttggagagtt agatactata
    11041 agtggaaaaa ctatatttag ttttccagaa gagcaattag gtcatgctca tgatacttta
    11101 ccaggtaagt ttatacatgg tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt
    11161 cgtaaaacac aagaatttaa agaaagaaaa acatcttata attttataga gccattaact
    11221 aaagaaaagc aagctggagt ttcattagga taataattaa aatactaaga ctctttttta
    11281 aggtatgtta gatgttatag gaggcttatt gtatgaatga aaagtcaagt tttaaacaaa
    11341 ttgaaattat tttagcagtt atttttttgg tacttacata ttacaaggtt agattaactc
    11401 cagcatttat tgttatattt ataccactat atttaagtta ttttgcagta tctatgattg
    11461 taaatcaaat agcaattaag ctattggaga taaggcaagc tagggaagct actaattaca
    11521 atataggaaa tggatagaaa gaaaaaagaa atattaacac tgtctatggg gattgcttta
    11581 ctacctcctt tatgggcagt attagcacct tatataggaa taaaaactgg agctgtagca
    11641 ctaatctgtg caggattata tgtaactaat ggtaataaac aaaaagatgg attaaaaata
    11701 atgtttggtt tttggtgtgg agatttatgg gcagtgttag ccattttaat tatgggatat
    11761 atgaatttca atcaaaacct agaattattt ttaacactta gtatattagg tttttttgca
    11821 gttgtaattg catccttatt tgaaaaaata attttcctac catcatggct ttgtggttgg
    11881 gctataggtc taactattat gacaggtgaa aatattatta atttacaaga tatttgtatt
    11941 caaattgctg tagctatggc agtaggagta tggtacgtgg gtgcaggtgt agatttgttt
    12001 acaatatata ttttgagtaa agataggaaa aagggaaatt gaaagcatgt gaatatactt
    12061 tgattatagg agaatggcta tgggttttga atttaaaata atgagaagtt taatatatgt
    12121 aggacttgcc aaggaagaat atagacctaa gctaatggac tggttatatc gtcaccatat
    12181 tccagatagt attagcactt ttggaccata ttgtactaaa tatgcctttt atcaagcata
    12241 tcctacacca aatgaaggtg agcgttttgg tgcacgtaag atgcaactaa cagaacatta
    12301 ttggcttgta gatgaacata tgcctgagat ggcaaataga attatgacag aatatatgcc
    12361 tatggatgtt ctacgttggc aagggtgtat accagatgta gaaaataaaa gggttcatga
    12421 aaatgcagaa agtggagatg caggacgtgc agtaggtgga gataatggat gtccaccatt
    12481 tatatttgcc tttgttccaa taaactggga agaagacttt agaggaaaag gacgtactgt
    12541 acaagatgga ccaaactatc gttggcaatt tatgattaag tatccagatg gtatctctaa
    12601 agaagaagga gaaaaatggt tctatgatga ggtagtgcca tactttacaa actgttgcta
    12661 tgttaatcgt tttgtcagta gtaaaataat gattaattat ggagcaactg cttttgaccg
    12721 tgtatcagaa ctatggtttg aaggggaaga agaatggtat aaagctgtgg ttgaagagac
    12781 aaagtcgttt attaaaaaac cagaatgggc acaagaagag gagttcccat atttaaaacc
    12841 acaattcaat atcgcatcag tattcttagg tgatatagca actatggatg catactcaca
    12901 gtatcgtgga tatataccaa tgagataaaa attatagttt gtgataagta gaggaggcat
    12961 ttactcttac tggtagaaga agacttattg tatccttaaa agtaggtgaa tatatagttt
    13021 tatatttaaa tttatataaa actaagacta ataaatatga aacaatagaa ttagaataat
    13081 ttgataatat ttgttaaaag agcatttaga atgtgtttaa agattaagat attcctttta
    13141 tggtagcaga tagttgaaat aataaaactg ttatcatgaa aggaatattt ttatgtatag
    13201 gaaattttta aaaataaaag ttaaatatgt ttaagaacat cttaaagtga aagaatcatt
    13261 gttagaaata tgtagacaat atagtcttta taacgtatca tattaacaag tttacacttg
    13321 gattatgaaa taaaaagagt aattccaaag gttgtaaata ctatgctgac tttgatactt
    13381 caaagtttag gtgattttgt aattgttaat agaaatacgt ttaatgagca taaagaattt
    13441 atctaataat aggagaatat aatgtataca attggacaag tatctaaatt tttaggaata
    13501 tctagagata cactaaaatt ttatgaggac aaaggattag taaatcctaa gaaaaatgat
    13561 gaaaatggat atagaatata taatcaggtt gatatctatg atattgcaac aataaacttt
    13621 tatagagaaa ttgatataga aattaaaaag attcaagaaa taagaaaaag taagagtata
    13681 aacaatttag aactcttact agaagagaag gaacaaatca tactaaaaga gatagaatat
    13741 aaaaagcttc tcttaaaaaa ggttcagtta gtcaaagaag attgtgacaa aataaaaaca
    13801 tattttggaa aatacacaat aaaagaaatg aagcctttgg aagtaaaagg taaaataaaa
    13861 catgttacta actatgagga atatgaagcc ttaaaagaga atacaaacaa cttaaaaaaa
    13921 gcagttacat taactgattt aagaagagta ataaatttta atgaagaagg tataatagaa
    13981 gaaaaattta taattgtaag ggaagtagat gactacaaca aaatcataga aggtgaagtt
    14041 ctatctcatc ccaattgtat ttatactatt attgaagatg gcagatggtc aactggagga
    14101 gaaaatattg ataataaagt ggaagacaga ttaagagaaa tagcaataga aaatgaatgt
    14161 gaattacttg gaatagtcta tgttaatata ttgcttacta cttatgaaga agggattgag
    14221 cgtatatttt tagagattta tgcacctata aaataatata aaagcattga cctgggactt
    14281 actcataggt ttaaactcat gatataagca gttataactt atatagcaat taaatattaa
    14341 tttgaaatga ggattactta tgagaacaga gaaatcatta aaaaggccta tagccagttt
    14401 tattattcta acaaatataa tttttttacc acttttttta ttggtagtag tgactataat
    14461 gttaaaactt ccaacagtga tatctgacat tgcgttgtgt atatcagcgt ggtcatccac
    14521 ttttgcattt atgatattat ttaaaaaaat ttatccaggt aagaaatttt tagtacacgt
    14581 aaaagataga tttagaaata aacttaaatt ttcaacagta tctataataa ttagtataca
    14641 agtcctaata gctgtagtag ttatatttat attagcatct aaaaatcatg gaataatgcc
    14701 taactttaca gtttcatcat ggggagcatt tatatattta tttttaaaaa atttatttgc
    14761 aggatcatta ggtgaggaat taggttggag aggatttgtt caaaatcatc ttcagaaaag
    14821 acattctcct ttaaaagcat ccataatagt aggtttttgg tgggggatgt ggcatttacc
    14881 aatatggttt acaacaggat tcaaaggact tgatttaata aaatatatta tattttttat
    14941 gattgcaatt atatcaatca gtattataat gacaactttt tataatctaa ataaaacttt
    15001 gataatacct attgtaatac atcaactatt taatttttta atagggataa ttaatggaga
    15061 tttaatagag cttataaatt actatgcaat gttatattta atatttgcaa ttgcattaat
    15121 agttataaat ccaggaaatg tattatataa aaatattaga gagtaatata tatctttatg
    15181 gtaagatata aagatattat tagaagaaca tccaaggagg aataaacatg gttttactga
    15241 ttgttgatgc acaaaagtta ataacaaacg agaggttata taaatttaat gaatttgtag
    15301 taaatgtaga aaatttaatt gatacagcta gaaaaaataa tatagaagta atatatgtac
    15361 gtcatgatga tggagttgaa aatgaattga ctaaaggtaa aaatggcttt gaaatatacg
    15421 aaaaatttaa accatgtaat ggagaaaaaa tatttgataa aaaagtaaat agtgctttta
    15481 aagaaactgg tctattagaa tacctaacaa ataatggtga aaaagatatt attattgctg
    15541 gtctccaaac agattattgt attgatgcca ctataaaatg tggatttgaa catggattta
    15601 atataatagt tccagcttat tcaaatacaa ctgttgacaa tttttttatg tcagcagaac
    15661 aatcatataa atactacaat gaatttatat ggaatgaaag atatgcgaag tgtatttcat
    15721 tagaggaaac acttagaaaa atgaaataaa aatagagtat gaattgtata tgtacataag
    15781 ttgatttcat aaaaaaggat gttaaattcc aagttaaaaa ttagattaaa tatattatat
    15841 tatagataag gcacatcaat tttattttaa attaatgtgc tttttaattg tagtaattta
    15901 tttataatca atatgatttt tattatttaa atcaattttt cttgagtttc tttgtctaaa
    15961 gaaacttttt ttatttataa aatctacagt tatattatag tatctatttt agagaagttt
    16021 aatttttgta tcaatctaaa acaaaagtta tacttaaatg aaattgaaaa cgataaaatt
    16081 ctcaaataaa ataagtattg tggaggtgat gaaagaatcc aatgataata tgaagactaa
    16141 tcttaataag aaagtagggg ttaatggtag tggacaaaga aattgtatca attctccaga
    16201 ttttattaaa tagcacattg attacaacta atggattaca agaggagtct aattcatcaa
    16261 agagacaaat tacttataga ataaataaaa tcaatgacat gcttaaagta aaaaatgtac
    16321 cacttatatg tttaagagct gataaagata ttatcgttag aaaagaaaca aaaaaagcaa
    16381 ttaaagaaat tttagaaaaa aactattcta agaatactta ttattttagt aagacagaaa
    16441 gacttttata tatgtattta atgcttttta ttaacatgga tgatatttca attaatcatt
    16501 ttataaattc tataaaagta agtaggtcta ctgtaaatct tgatttcaag gatttgatac
    16561 cagagcttga aaagaagaac attaaagtta agaacaatcg tataaatgga tattacatag
    16621 ttggcaatga gatggaaatt aggagagtgc ttattaaaaa tattatagaa actttgtcta
    16681 atgagaaaag ttcaagagta tttgatattt ttattaaaga atataattta gatagttttg
    16741 aagaggctaa aaaaattata ttaaagctaa taaaaaaata tgaaattact tttgttgaag
    16801 atagattagt agaatttata tatatattta tctttctaaa ggcaaggatg tattcaacta
    16861 gaatagttac tcaggaaaat gtagatattc ctaatatttc agttatgagt tcaatgaagg
    16921 agtataaatt tgctaaggaa ttattagaag tatatgggag tgaagataag ataaagcctc
    16981 acaatataat gtatattagt gcttggattt taggtatatc agtaggaaat tatgaagaag
    17041 atacagaaga taaggatgta atttcaaaaa ttgtaaataa aatgatgaca aagtttggat
    17101 atttaagcgg agtttactat ataagccaag aaaaaatatt taaacaatta tattctcatt
    17161 ttagacctgc ctactataga cttttattta aattacctat ttataatccg atttgtgaaa
    17221 aagtaaaaga agaatataaa ttagtttata gaattgtaag taatgcaatg aaagagtttt
    17281 gcggattatt tggagaagaa attcaggaag aagagttagc atatcttaca atgcactttg
    17341 caacactttt ttcaaataaa aaagaatttg atggttttag aaaaagaata gcattaatag
    17401 tttgttcaaa tggcgttggt agttcagcta ttttacatgc agaacttaca aacttatttc
    17461 ctgacttaca ctttatacca atgtcggaat cagttggatt agaaaatata agtgaacctg
    17521 tagatattgt atttactact aattataacg cagagggttt aaaaacagat gtaccagtaa
    17581 ttaaagtaag ccctgttatg accacaaaag aaaaatataa gattacacgt gaagtgtata
    17641 ttcaattagg tgatattttt ttaagacaac ctaaaataga tgaggttatg gatataataa
    17701 aaaaatatgc aaatgtaacc tctgaaagta ttctttcaag tgagctatta gcttatttta
    17761 cacagataga gaattttaca tctaaggaag gagaggggcc tatgctaagt gagattacta
    17821 atgaaacact agtaaaaata aaggtaaaag ctaagaattg ggaggaagct attagacagt
    17881 cagcttctgt attagttgaa aataacaaag taactgaagg gtatgtagat gctatgatta
    17941 actcagcaaa agcatctgga ccatacatag taattacaaa acatgttgca cttccacatg
    18001 ctagaccaga agctgggtca aaagagattg caataggcat tgctacttta gatagtccta
    18061 ttgagtttgg gaataacgaa aacgaccctg taaaatatgt attttgtcta agtgcaatag
    18121 acaataatag ccatttacgt gctatgtctg agctagttga actattggaa gaagatgagt
    18181 tttttaaggt tttagatagt gctaaagaag ctaaagaaat tattgatttt ataaaaacac
    18241 acgaattata aaggaggatt taattatgta taaaatttta atcgcatgta gagcaggagt
    18301 tggctcaagt ttaatgttaa aaataaagac tcaacaaatt attaaggaaa ataactttcc
    18361 aattgaagta gaacatggtt cattggattc actaaatgga ttcaatggag atgctgtaga
    18421 aacattaatt gatgttgctg aagaattaaa aaataaaaat ttgaagtttg atataattgg
    18481 gattcacaat attgttgacc gtaatgaaat aaagacagca ctagaaaaat tccttgcttc
    18541 aaaagaggga taaatttaag atgataagga ggaatattta tggagtttat tattagtttg
    18601 ctgagtacac ctgctgtttt gcttggcgta gttgcatgta taggattgat tcttcaaaag
    18661 aaatcagctg ttgaagtatt tacaggctct tcaaaaacac ttatcggttt tttgatattt
    18721 ggtattggtg cctctgctat gacaggagca cttcaaaatt tcaataaact gttccaacat
    18781 ggctttggaa ttactggtgt tatagcttca ccagaagcag ctacagcact tgcacaaggt
    18841 acatatggtt ttgcagtttc atgtacatta attcttggat tcatattaaa tttagtattt
    18901 gcacgtatta caaagtttaa gaatattttc tttacaacag gtcatagttt gttcttttct
    18961 tgtgtattag ttttaattat gaaagcacat ggatttgata atacaatcac tattttaata
    19021 ggtggaacta tcttagggtt tatgtctgct gcactacctc aattctgtca accatttatg
    19081 cgtgaattaa caggtggcga cgaacaagct attggtcact ttaacatgat tggttatggt
    19141 ttatcaggtt atattgggcg tttattttca aaacataaag atgatacaac agagactata
    19201 gaatttccta agtggttatc aatattcaga gacttcttaa tgggtctatc catagttatg
    19261 ttaatcttat tttatattgc aacattaaaa gctggtcaag catttactca agaaattgct
    19321 ggttcaacac attggttggt ttttccgctt atagaggcat ttacatttgt ggcaggtatg
    19381 tcaatattga tgtcaggagt tcgtatgttc ttagctgaaa taactgcagc atttgtaatc
    19441 atttcagaaa agtatattcc aggttcacgt cctgcgctag atgttccaac cgtatttcca
    19501 tatgcaccaa atgcagttat tattgggttt atatcagcat atgctgctgg tctattagca
    19561 attgctatta tggcatcatt tcctagtata tttcctgtag taattattcc agctgctcac
    19621 atatgtttct tctcaggagg tacagctgca atatttggca atacttcagg aggatggaga
    19681 ggggctattg ctgggtcatt tgttgtgggg ttattacttg catttttacc agttatacta
    19741 tatccagttt atgggactct tggtatagaa ggagcaactt tccctaatat tgattacaac
    19801 gtagttggtt cgatattaca taatatttta caatttataa aacctattta gactaaatta
    19861 tataaaagta tagtcatagt tactatgatt tcaaggagga tacatcttat ggaaaataca
    19921 ttatctgaat ttttagataa tatatctaat gaattgtcag aatttattgc ctcaatagat
    19981 gagacatcta taaataaagc atgcgaatta attcttgaag ctgaaaaaaa tcacggacgt
    20041 gtacatgtga ctggtattgg caaaccagga catgtatcgg gatatatttc atcattgctt
    20101 tcatctactg gaacgtcagc atatatattg catggtacag aagcagtaca tgggtcaagt
    20161 ggtcaagtag ttgaaggaga tgttgttatt gcaatatcaa atagtggtga aactcaagag
    20221 ttaaaagcaa ctcttaagac tctaaaagta aatggagcaa agataattgg tgtaagtggt
    20281 aatgagagtt catttttaaa aaatatttct gatatattcc tgtttgcagg tgttaagcag
    20341 gaaggagatt gtctcaataa agctcctcgt gcttcaattt tagctgaaac aataatatta
    20401 caatcattaa gtgttgtatt acaatatgct aaaggtctta atacacagga gtatttgaag
    20461 tggcatccag caggaagtct tggaaagtct attagagagg gaatataatt ttatgaaata
    20521 taaagggatt atatgtgcaa tgataacacc atttgatgaa aatcaaaata ttaattcaca
    20581 agcaacatgt caattaattg attatttgat tgaaaaggga atttatggat tatttatttt
    20641 gggcactaat ggtgaatgtc atgtattaac agatgatgaa aaagttgaat ttgcaaagat
    20701 tgttattaat catacaaata atcgtgtacc tgtttttgtt ggaacagggg ggaattcaac
    20761 tcgtgaagtt gtagatttat ctaagagaat ggaagcgatt ggagcaagtg cattatctat
    20821 tattacacca tactttgtta caccaacaca acaggaatta atcttacatt acaaaactat
    20881 tgctaattca gttaatttac ctattataat gtataatatg ccaggtaaga ctggaattaa
    20941 tattgaacca gagtcagtac gtgaattatc aaagataaag aatattgttg gaattaagga
    21001 tagtagtggt aaacttgaca acatgaaagc ttatattgaa gcaacgaaga atgagaattt
    21061 tagtgtgttt tcaggctcag attcacttat tctggatagt ctaaaagctg gtagccaagg
    21121 agctgttgca gcaacaacaa acttcctcac agagattggt atctctatct atgaaaactt
    21181 tatgaaaggg gatttggaag aagctcaaaa atctcaaagt agtatagaag aacttcgacg
    21241 tatactgaaa cttggaacaa ttccatctgt tatgaagaaa actattgtct tgaatggaat
    21301 taatgttgga actgcacgac ttcctgtaac tgaaccaact ggggaagtac ttgaagaaat
    21361 taaacgagta gttgaaaatt ataggacaat acttaataaa agtagtgaga atagataata
    21421 taaataggga aattacactg gtatataatt aatatatata ttataaaaca tgactaaaat
    21481 taatgaataa gtaaagtggt ttgatgagta tagaggtgat aatatgaaaa agatgaatat
    21541 aaccaaaaga atgtcagaat gtggggctct tgcaattgta agagaagaaa atcttaaccg
    21601 tgcatgtgaa attgctgaag gatgtataaa aggtggtata acagttatag aaatgagtta
    21661 taccctaaat aatgctggag aaataattca agggcttaat aaaaagtatg gtgaaatact
    21721 ttgtgtggga gcaggaacag ttttggatag tgaaacagct cgtcatgcta tattacatgg
    21781 agcccaattt attattgcac caaattataa tgaaggtgta gcgaagattt gcaatagata
    21841 tcagattcct tatgctccag gatgtacatc attgactgag gcagtagacg cactaagttt
    21901 aggagcaact tttataaaag ctttcccaat atcagatttc tatggaccta aactagtaaa
    21961 ggtatttaag acaccaatac ctgatatgcc agttttagca agtggagggc ttaatattga
    22021 aaacctacat atatggttag aaaatggagt tgatgtatgt ggatttggtg gtttattaac
    22081 taaaggttct gttgaagata ttgctaaaaa tgctaacaag attcgtgaaa tcattaaaaa
    22141 tgtaagaaaa atttaacata gggctgtatc atagtatctt ataattacta tgatacagcc
    22201 tttttaatat tattcaaatt taaaacagtt tacatttcta ttatattaca tataatttca
    22261 gtaattttat cttaattttt ctatctggaa aaaaatgatt taaaacttaa gttatgatat
    22321 aatttcatta aaagaaggcg atttttagta aatatgagaa aataatgtgt ttttagggga
    22381 aaagttaaat acagatttaa gtgaacaaga atcgataatt aagatattaa atggtgaata
    22441 gggcatcaaa aaaattacta aattaaaagt tttagaggga gaatcttagc aagtgtgtgt
    22501 gaaaattcaa tttcaaacaa aaataattgt aaatgaaatc aatttataca cgttgaagat
    22561 tcacttttat cattatatta aaaagcagag tgaattaaaa gtgagtctaa agataggagg
    22621 ataattaaaa caatgaaaga agacattagg ttacatcgaa tgtgtaaaaa agaacaagca
    22681 acaaagaatg aagttatatc agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa
    22741 cattttttag atgattcatt tacaataata tggggaaata catcattcta tcaattatta
    22801 ggatatacag aagaagaatt tttagttaaa ttttctagtt taaaagatta ttatgaacag
    22861 aatctttatc attttgaatc tatgaaaaat cattttttag aggcttatca tcatggcgaa
    22921 aagagtatag aatatcaaat ttgcatttca gcaaataatg gagaccctgt ttgggtaata
    22981 atgactggta tattaattga atcacaagat aatgagaaat caatggtcta tatattgtat
    23041 tcaaaaatta atgagttaaa aataaagcaa gaagaactat ctgatatgga aaaagagagt
    23101 acagagaatt ttaaatggat gatgtcagag tatgctggga atgtttatat tagcgatatg
    23161 gatacatatg aattactata cttaaataag cactcatgca atactttaca ggcatcagca
    23221 aatgaactta ttggaagaaa atgttatgag gtaattcaag gtagaacttc gccatgtccg
    23281 ttttgtacaa attcgtattt aaaaaaagat gacatatatg aatgggaatt ttataataaa
    23341 aaactgaaac gaacatttat aattaaagac cgtatgttga attggcaagg gcgaagagtt
    23401 cgtattgaat tatcttatga tatgtatagt gctgaatata aattagcaaa aaaagatcag
    23461 gaaagagaat caattttgaa aactattcca gcaggaatgg ttagattaga tgcaagagat
    23521 tatcttacta tactttggtg taatgatata tttttgaata tgattgaata tacaaaggaa
    23581 cagtttgcag aggaacttaa taatcaatgt ggatatttat tttctgaaga ttataaacgt
    23641 agtaagaaac tggcacaaga tttgaaggag tctggggata atgttgtatt tgaagcaaaa
    23701 atatatacac gttcaaggga agaacgtata tggactgtta cattgtgtta tatcagtggg
    23761 gaagacagtt gggatggaat accatctttt tatagtatag gtattgacat aacaaaggaa
    23821 agaaaacaga ttgagaaatt acagcatata tcagagaaag atgcattaac aggaatttat
    23881 aatcgtgctg agactgaaag gcagattaag aaatattttg aaaaaaattt aaatgttatg
    23941 ggtgcactgt ttatgattga tacagataat tttaaacaga ttaatgatac agaagggcat
    24001 atgattggag atatagtact tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac
    24061 agtgatgttg ttggaagaat aggtggagat gagtttacaa tttttatgaa gaatatttcc
    24121 tcagttaaag atgcagaaaa gaaagcagaa gaactattgt atatgttccg tcatctattt
    24181 cagaaagaaa aaagttttct aaaagtaact tgtagcatag gaatagctat atatccaaag
    24241 gatggtacta cattcaagga aatatatgca agagctgata aagccctgta tcaagcaaaa
    24301 aatatgggaa aaaataatta tgtaatctac aaccaagatt attttaaaga attagaagaa
    24361 tttgattgtt cttctcttgg tacagttatt gattcagaaa aacaatacgc tgaatatcct
    24421 gataatctta cacgttatat attccgtatg ctttatcaga ctgatgatat agaccaggcg
    24481 attaatatga tacttgaggt tgttgggaaa cagtttgatg taagtcgtgt ttatattttt
    24541 gaaaatacag aagatggtag atatacttct aatacttatg aatggtgtaa tgaaggaatc
    24601 tcttctcaaa tggcttattt gcaaaacagc aattatcaag actatgagga ttatgaaaag
    24661 ctttttgagg atgaattagt tttttattgt cgagatatcc atacattaag tcctaaacta
    24721 gaagaattgt tttctagtca aggtatccac tctacacttc aatgtgcata taaagaagat
    24781 agagttttca gtggatttgt tggatttgat gaatgcacag gactacgtct atggtcacaa
    24841 gaggaaataa gtacattatc attgatttct caaattatat caatgttttt acaacgtaaa
    24901 aaaaataata aacttaatca acagattcaa caataccaga atattttaga taatattgac
    24961 cagtgtattt gtgtgattaa taaagaaaat aattttttat tatatgtaaa taaaaaattt
    25021 aaaaatacat attctaaatt ccaaattgga caaccttatt atcttgattc tatagatatt
    25081 cataagatat ccattatatg gaataaaaaa gaagcatacc tatgtactag cacgatttaa
    25141 tggtaaagta aagtttatat tggacttaaa ttaaatttcg ttttaaataa gatattattt
    25201 tagttgctta ctgaaaagac tagaatctat attaatgaat tatactaatt ttataccact
    25261 attaaaatat tgtttgctaa aaataaaaaa tagccttaaa acagttgatt ataaaaaatt
    25321 aactgcttta aggctatttt tatctttgtg acgaaataga cacttgccat ttagaatgtg
    25381 acaatatagt agttgtttag aaaaggcaca aagcattata ctttaactaa agtaaaatgt
    25441 attttgcttt tagaataaaa acaattagga gggagctata attgaaaaag ataatatatg
    25501 tattatttac aattgaacta ttaatagtat ctttattagg tctaaatata gttaaagata
    25561 atgaaattaa taatatttta tataacgata caacaagtat atctgtaatg tttaaagatt
    25621 ataaaaagtt aaataaaaat tatagtaagt ggattaaaga tattgcagat gaaaataatg
    25681 tggctatatc aaagtatgtt tttaataata atgaaaaatt atctatatat acgactgata
    25741 caagtttgaa taacaatata aagttaaaat caggaaaatt tcctaatact aattcaaatg
    25801 aatttattag taatataaac actaataata gtaatcaagt aggtaaattc tcaactatga
    25861 gtaaagacat atcaatttta attagggatt ttggtaaaat aagtgaagtt ggagaatcag
    25921 gaatcttata tatttcaact caaaatgaag atacaataaa taaaatatta agagaactaa
    25981 atgtagacag aagtattttt gtagcaagat tacatgatag atatgttaac tcaaatttat
    26041 acttaaatcc ttcaataatt agagatttaa tactaattat cctttgtttc ttggctacaa
    26101 taatacatta ttcaattagt gtgagtagag aggcttctat tttaagatta aatggatact
    26161 ctaagctaga tataattgct agagttataa aaaatatact tagaattatg attttatcat
    26221 ctgtcacagc attattaata tatataatct atattttaaa attaaatata ggtgttagag
    26281 catgcttata ctttgctatg ttttcgatta tatgtatttt gataaatatt ttgatatcta
    26341 tattaatagt tggttttaat agtagaagtt ctaaatatgt gctatctcta aatggaaaaa
    26401 aatcttatgg ttttgtaaat ataatgcatt tcacattgaa aattgtattt gtatcatttc
    26461 ttatattatc cataaataac tgtattttaa attataatat gctacaaact cagttaggaa
    26521 atctatctga gtgggataaa gcaaaaaatg tttataatct tactcttaaa gatacaggcg
    26581 aacaatcttt aggaaaggaa gaagtaatta aaaatgccaa gattaaaggt ttctataaaa
    26641 atttggtaga agaaaaagat gctttcttaa tagattcaac taattttgaa aaacttgaag
    26701 atggaagtta catgtatgaa ataaattcaa agggtaaaaa cccagaagta agtcaatatg
    26761 gtaaaaatat aaaaataaat aaaaactatc ttaaagttaa ccctatttac tcaaatggca
    26821 aagaaatatt taatctaatc gattatgacg ataagactat aaatttatta gttcctaaaa
    26881 agttacaagt acatgaaaat gagattaaaa aagagtttag agaattattt tattttgaaa
    26941 aaatagaagt agaaaatatg tacaataaag agttaaatag agagttaaat aaaacaaaga
    27001 agtccgattt aaatgttaat ataatatatg tagataataa tcaaagctat tttacttata
    27061 actcccttgt tatggatgac aataggaatc tcattgatga cccaatagca atagttgaca
    27121 tagataatat tgatgattct ttttatcttt cttatatatc tagatgtgta tattttaatt
    27181 ctaagaaatt agatgccctt gcagatataa gtagcattat tgaagctcaa ggtgtagaag
    27241 cgcatatcca aagtttacat gctgtttata atgagtatgg tttagaaata aataaactag
    27301 aaaaatattt gaatagtgaa atttttacta taataataat agcaatatca aacttaatga
    27361 ttacctacaa tatagtagct agctactatg aaagaaataa atataagtta tatattaaaa
    27421 aaatatttgg ttactcaact acagctagaa gtatgttgtt aatagtctca ttaatattaa
    27481 caaacattat tccaataggc attatatcaa caagagtaga tttaagtaat aatattatct
    27541 tatttggact tctaatatta gttatagaag taattgtatc tattgtatta gacaagatta
    27601 tctcaaatag ttcatttaat aaaattataa aaggagaaca ttaaaaagat gatttcatta
    27661 ataaatatat ctaaaaaatt taaagataaa tctataatag acaaatttaa cctagaaatt
    27721 tctaagggcg agtttgtagc gataacagga aatagtggtt caggtaagac aactttactt
    27781 aatataatgg gattattaga aaaacctaat tctggtgata ttataataaa taatataaaa
    27841 aatccaaata gtaaacagat aagaatttta caaagggatt tttatggcta tctatttcaa
    27901 aattatgctt taattgagaa tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat
    27961 caaaaaaata tcaatcataa tgaaaaaata aattacgcac ttgagtctgt aggtcttaga
    28021 aaatacaata aaaaaaagat atacgaattg agtggaggag aacaacaaag ggtagcatta
    28081 gcaagagtaa tattaaaaga atgtgaagta atatttgcag atgaacctac aggtaattta
    28141 gataaaaaaa atagagacat agtatttgat atacttaaaa gtctaaataa gaatggtaaa
    28201 actatcgtat ttgtaactca tgatttagat ttatcggagc aggcagatag agttataaaa
    28261 ttgtaataat ataaactata ataaatataa gaaagagatt acagctaaat taagtttaat
    28321 aataatacga atagaattta aacattaagt aaattagtag tttgtaaata taagtataat
    28381 atgatatcat aaaggctcaa aagaaatttt atataataag aaatctaaaa ataacaaatg
    28441 gaggacatat aaagatggaa aatttaccaa attgcccgaa atgtaattca gaatatactt
    28501 atgaagatgg aactctttta gtttgtccag aatgctctca tgagtgggca ttaggattgg
    28561 gagctgaaga agattcaaat attatcaaag atgtaaatgg aaatgtatta aatgatggag
    28621 attctgttac agtaattaga gaccttaaag taaaaggaag ttcatcatct ataaagattg
    28681 gaacaaaagt gaaaaatatc cgtttaatcc atgactcatc agatggacat gatatagaat
    28741 gtaagataga tggttttgga gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat
    28801 attataaata agctgtagtg tttattgttt ttcagtacag tataaaacca taagaatcta
    28861 ttaagtaata aaagtttgtt tatttatctt tatattttct tatggtttta ttaaatataa
    28921 attttattga acaccaaaat attctctcct ctacaaataa gccaatctta tttaaactaa
    28981 tatactttgt actatttata ctgaaattgt aaatttaaat gctatgttat ttag
//
LOCUS       DAMJZE010000031        26646 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJZE010000031 DAMJZE010000000
VERSION     DAMJZE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.031"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            85..1827
                     /locus_tag="PC541_001256"
     CDS             85..1827
                     /locus_tag="PC541_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HDF2838727.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1997..2185)
                     /locus_tag="PC541_001257"
     CDS             complement(1997..2185)
                     /locus_tag="PC541_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838728.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2182..2763)
                     /locus_tag="PC541_001258"
     CDS             complement(2182..2763)
                     /locus_tag="PC541_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2838729.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2931..3749
                     /locus_tag="PC541_001259"
     CDS             2931..3749
                     /locus_tag="PC541_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2838730.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4759..5580
                     /locus_tag="PC541_001260"
     CDS             4759..5580
                     /locus_tag="PC541_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2838731.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5890..6669)
                     /locus_tag="PC541_001261"
     CDS             complement(5890..6669)
                     /locus_tag="PC541_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2838732.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            6919..7986
                     /locus_tag="PC541_001262"
     CDS             6919..7986
                     /locus_tag="PC541_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2838733.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            8156..10057
                     /gene="cooS"
                     /locus_tag="PC541_001263"
     CDS             8156..10057
                     /gene="cooS"
                     /locus_tag="PC541_001263"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2838734.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            10050..10523
                     /locus_tag="PC541_001264"
     CDS             10050..10523
                     /locus_tag="PC541_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2838735.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            10498..11724
                     /locus_tag="PC541_001265"
     CDS             10498..11724
                     /locus_tag="PC541_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2838736.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(11863..13050)
                     /locus_tag="PC541_001266"
     CDS             complement(11863..13050)
                     /locus_tag="PC541_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2838737.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            13323..14147
                     /locus_tag="PC541_001267"
     CDS             13323..14147
                     /locus_tag="PC541_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2838738.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            14276..15541
                     /gene="gluD"
                     /locus_tag="PC541_001268"
     CDS             14276..15541
                     /gene="gluD"
                     /locus_tag="PC541_001268"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2838739.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15754..16344
                     /locus_tag="PC541_001269"
     CDS             15754..16344
                     /locus_tag="PC541_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2838740.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            16675..17808
                     /locus_tag="PC541_001270"
     CDS             16675..17808
                     /locus_tag="PC541_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2838741.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            18724..19746
                     /locus_tag="PC541_001271"
     CDS             18724..19746
                     /locus_tag="PC541_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2838742.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            20042..20962
                     /gene="pyrB"
                     /locus_tag="PC541_001272"
     CDS             20042..20962
                     /gene="pyrB"
                     /locus_tag="PC541_001272"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2838743.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            21001..21693
                     /locus_tag="PC541_001273"
     CDS             21001..21693
                     /locus_tag="PC541_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2838744.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            21686..22588
                     /locus_tag="PC541_001274"
     CDS             21686..22588
                     /locus_tag="PC541_001274"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2838745.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            22673..23257
                     /locus_tag="PC541_001275"
     CDS             22673..23257
                     /locus_tag="PC541_001275"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2838746.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            23471..24397
                     /locus_tag="PC541_001276"
     CDS             23471..24397
                     /locus_tag="PC541_001276"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2838747.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(24449..25339)
                     /locus_tag="PC541_001277"
     CDS             complement(24449..25339)
                     /locus_tag="PC541_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2838748.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            25527..26558
                     /locus_tag="PC541_001278"
     CDS             25527..26558
                     /locus_tag="PC541_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2838749.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
BASE COUNT        10353 a         2998 c         4379 g         8916 t
ORIGIN      
        1 ttatgttaaa ttttcttaac atgcattttt tatatttaat atagtaaaat aattaataac
       61 tatatccatg attggaggat ttttatggtt aaatccaaga agaaaatatt tgtttttcta
      121 attttaattg cagtttttat tatatttgca tcatacataa gaaattatac taatgataca
      181 aaaaaagatt ctgttagagg aactattcct tttgaaaaaa tggaatataa gcgtcctaat
      241 atacagtcaa tttgcgaaaa cattaacaag tgcaacgaca aacttttaac agctaaaaca
      301 gctaaggaac aattaaattt atttaatgaa gttgataaaa tttatcaaga tttttacagt
      361 acacttacta tagcaaaaat tagaaataac atagattcat cagacgaatt ttactctaag
      421 gaatataagt atcttatgag taaatcagta gatgttgaca tgtcatacaa agactttcaa
      481 gatagatttg taacttctaa attctctaaa gaattagaaa aagaacttgg aaaggataca
      541 tttaattatc taaagaacat tggaaaactt aattctaaag aagttgaagg tttgcttaaa
      601 aaagagcaag acctagttgt aaaatatgaa gacttattag ctaaatctac agtatcaata
      661 gatggtatag aggtagactt tgaagaagcc ttgtcaagac caaatcttag ccctgaagaa
      721 tatgtgaaaa tttattcaga ttacttaaaa aaatacaatc ctatatttgg taacatattc
      781 ttagaactta tacagacaag aactgaaata gcaagtaagc aaggatttaa gaattatata
      841 gattatgcct atatgaacct aaacaaagac tattctcaag aagaagctaa aaaatttaga
      901 caagatgtaa aagattacat agttccatta tatagagaga tttcatctaa accatctgat
      961 tcttctattt atattaaagt atataaaaat cgctcattta ggaagtttga taaagtactt
     1021 gaagatatat ctccaaaact aaaagagagc ttcgactata tgaaaaaata tgacttgtac
     1081 gattattctt ctggaaaaaa caaatcacct ggaggatata ctacttatat aaataaatat
     1141 aaagcacctt tcttgtttaa tacatgggat aattcttttt taggtgttac tagttttgct
     1201 catgagtttg gtcattttta taattactac aacagtatta acttaaacaa taagatgcaa
     1261 ccaagtatag atgtatgtga agttcattca acatctttag aaattctatt ttataaatat
     1321 tttgatgatt tttttggtaa acaatctgaa gctataaaaa aagaacactt atcgattgtt
     1381 ttaaatacta ttattgatgc ttgtctttat gatgaatttc aagaaattat atacaagaat
     1441 ccatatatga gtctaaatga tatcaataaa ttgttttttg atttagagga agaatatgga
     1501 gtttccaata atatacttag agaaaaaaat gctccttttt ggatacttgt atctcataac
     1561 ttccaagttc ctttttatta cttgagttat ggtcttgcat ctgatgtatc acttcagata
     1621 tggcaattat cacaagaaga ttacagaaaa gctgttgatg tatatatgga ttttttaaat
     1681 caaaatactg atgctggatt taaagatgtg gtagaaaagg taaatttaca attacctttc
     1741 caagatggaa atttagagga aataagctct actttatatg attattttgg tatagataat
     1801 ccgcttgaac taaaaaatgc ttcttaattt ctaatacttt atactcactc ttaagattta
     1861 gtattagatt gaaataagtg atattttttc acacacaatt aatgtttaat aaaattatat
     1921 ggtaaagtat cagaaatttt taagactctt gtctttataa tttctgatac tttatttatt
     1981 aataatatta ttatttttat atatatataa ttaaaaggat tgtggaaatc aataaaaata
     2041 tccctgtaaa aatagcacta ttcatatgga acttaataga acttgcatct gttaccttat
     2101 tattcttaat ccagaaccca caaatcatag ttgaaagaaa aagtaaaaca gtgataagac
     2161 ttgaaccaat agttataatt ttcatcttac atcaacatcc tctccaataa tattttctat
     2221 taaaatatca atgaatttat ctcgctcata tttattcttt atatttatac cactgtataa
     2281 ctgaaatgat tctgtagata aagcagaaat ctgtattggt aaaataattt gcagggcaat
     2341 aattcttaga ctcatttcat cttttttatt tccaaaaatt ttacgaagca tagggacaat
     2401 atcaagttct gcttgcatat ctccattggt tatacattga ttcaatacaa aaggaagcac
     2461 tttttcataa tgtaatccag tatcacacaa tttttttaat aaatttttta aatcttcaat
     2521 aggtcttaga gtatatacac tatcgtcata tagctctgct attatatttg acatcacatc
     2581 tcctatggca gtactcaaca aattatcctt tgttttaaaa tgataattta ttaaaccaac
     2641 tccaacattt gcacgctcag caatttttct aactgtaatc tcctcaatat catctacttc
     2701 atttaaaaga ttcaatgtaa cttcaattat acgttctcta gcatctttat cattactttt
     2761 cataatagac ctccaaaaca attaacattt tcagttctga acatgttcag tataatattt
     2821 ttttcatcat atgtcaatat aatttaatta taattttagt tgactctaac attatgatat
     2881 ggtttatatt aatattaagc taaaagtacc tttataaagg agacaaaaac atgtttaaga
     2941 taggcgattt ttcaaagcta agtaaaatta gtattcgtat gttacgtcat tatgatgaaa
     3001 taggactttt aactccttct catacgaata aaactaatgg atatagatac tatagtgcag
     3061 accaactttc tactacaaac agaatccatg cactaaaaga tatgggcttt ggcttgtatt
     3121 ctataaaaga aattttgaca gaatataacg ataaagaaag tctcataaaa tatctaaata
     3181 tccaccattc tcaagtaaaa gaacaattag aagatacaca gaaaaaacta ttaaaaatag
     3241 aaaccactat taaaagaatt ggaggaaatg atattatgaa aaattatgat gtaactataa
     3301 agaattttgc accaaaatat atgatgacac ttagaagggt tataccaact tatcaagacg
     3361 aaggaatgtt atggcatcaa gcatttttag agacaaaaga ccagaatgtt caaattgaac
     3421 ccccaaagta ttctaaagca gttttttatg atactggata taaagaagat tatgtagatg
     3481 ttgaagtaca agttgctgta tctggaaagt ataaagatac tgaacatgtt aaatttaaga
     3541 ctgttccaag tgtgactgct gcaactgcaa ttgttaatgg aaatttcaat caagttgcag
     3601 acgcttgtga ggctattggt aattggatat cagataataa ctatgatgtg gatggtccta
     3661 tgtttaatat ttatcatgta agtcctggca acgatagtaa tcctgataat tgggtaacag
     3721 aggtatgctt cccagttaaa aagaagtaat ttttatatta attggataat attttaattt
     3781 gttagacaaa ttacttttag tgttatgtat aaatacctac aatttaatta ggatattatc
     3841 ttaatttgtt aaacaaatta cttttagtgt tatgtataaa tatctacaat ttaattagga
     3901 tattatctta atttgtcaga taaatcactt gtgatgttat gtttgaatga ctagaattta
     3961 atcaggatat tatcttaatt tgtcagataa atcacttgtg atgttatgtt taaatgacta
     4021 gaatttgatt gggatattat cttaatttgt cagataaatc aattgtgatg ttgtgtttaa
     4081 atgactagaa tttgattagg atattatctt aatttgtcag ataaattatt tataatgtta
     4141 tgtttaaata cctacaattt aattaggata ttatctcaat ttgtcagata aatcaattat
     4201 gattttatgt ttaaatgact agaatttgat taggatatta tcttaatttg tcagataaat
     4261 caattataag attatatact atccttatac tctagttaaa tatattttaa tcactaatat
     4321 aaattagttt ttttaacaaa acaataaaag agtaaagtac aaataattcc tttgtactct
     4381 actcttttta actttctttg tacaataata caatgataaa tataaaaaat tatatattcc
     4441 attctataga aatatgctca ccaataacat gtatgttgtt aatcaatata ttatatcaaa
     4501 ttatatcaaa attacataaa ccttatagga caatcatatc ttattacaag aaaaatgtct
     4561 tatccatatt gaataagtca ttcagttcat atacaaagtc aatttcattg agaaacattt
     4621 aatatatgtt tataaaaatt tcatactatt ctttcatatt tacatgtgat ataatttatt
     4681 aactcataat tttgttaatc agttgtgctt gtatattatt tatataatct aatattttta
     4741 gaaggtggtg taataacttt gaaattaaaa attgttggta gtggcggtat gtcaattatt
     4801 ccaagttcat tttgtaaatg caaaatatgt gaagaagcca ggcaaaaagg cggaagatat
     4861 gaacgattag gaccaagttt atatattgat gatataaaaa tgctgataga cacacctgaa
     4921 gacattgcag tagcttgtaa cagacaaaaa atatcggaag taaaacatat ttcaatttct
     4981 caccatgacc cagaccatgt taagggtatt agaattgtcg aaaaaatagg atgtgacttt
     5041 atcacaggag aaaataatcc aattggcttt tatgcgcttc ctgaagtaat tgaagatata
     5101 aaccgaacga atctcgattg tcttaaatat tatggtgatg ttataaattg catttctatt
     5161 aatgaaactt cgcatgtaaa gataaataat tttgatattg atttgattaa caataaccca
     5221 gacaggaata taaccttcta tgttatcaaa gagaatgaaa aaaaagtcat atatgcctgc
     5281 tgtaatccca agccatttac tcatagtgat atgtactttg atgctgatat tttaataata
     5341 agtctagttt ctgatgatgg tatattaaat gatggtacaa aacttaaaga cacaccattt
     5401 aaagatgaaa tatttacttt agatgagatt gttgaaatta aaaactatta tagaattaaa
     5461 aaaattattg ttacacatat tgacgaaatg tggggaaaat catatggtta ttacagtgaa
     5521 ttagaaaaga aacttgataa catttatttt gcatatgatg gtatggaaat tatagtctaa
     5581 atcattaagt aataagcata atgcaaaata agaaaacttc aatttaacag tatagtcaaa
     5641 ctatgtgata tcaacatatc gcagttttta caaaatatca tagtatttat ctttataaaa
     5701 tttcaagtcc tacatatttt aatacttcaa tatctaaata aagtaaaatc aaaaatatat
     5761 tttatataca ttatagacgg cttatcgtca ctagataagc cgttttgtca caattattat
     5821 ttgctcaaca aaacttctca taatatataa cagattgaat ctccaactga tacttaatta
     5881 ccaaaactct tattccttaa tatgttgttg cattaaccaa accttcatca tcattttttg
     5941 tggcaatagt tttgctaata aatgactgga ttttgtattt ataccataca cagaaatatc
     6001 cttatttttc tttgcatcct tcaatgcttt tttggcaaca acgtctggag acactatgcc
     6061 tgggaaattt tttgtccctt tttcagctac aactatacct ctcttaaata gatttgtact
     6121 catccaacct ggacatacag caattgcatt tatcccttta ctcttaagtt caacatttaa
     6181 tgctcttgta taatttctta taaacgcttt agttgaacta taaatatttt gatatggcaa
     6241 tggctggaat gatgcttgtg aagaaatatt catgatatgg cttccctttt gcatatatgg
     6301 aatacatata agtcccaatg ctacaacagc actaatattt aaatcaatca tattaataga
     6361 ttcatctata cttaaatcat tgtatgaaca gaattttgca aaaccagcat tgtttataag
     6421 gtattttata catacatttt ctttacttaa aaatgtacca atttctttta cttcttctat
     6481 ttttgataaa tcttttgaaa aaatttttac cttacttcca aattcatcta ctaattgatt
     6541 taatctcgcc ttgtttcttg caactgccca tatttctgtt atttcttctt catttattaa
     6601 taactttaga aactctttac caaaaccact attagcaccc gttataattg caatcttctt
     6661 ataactcata attattctcc tttaccataa gtttatacgc tcaaactgta aacttatttt
     6721 ttacaaataa aacaccctct attcagatgt tatattcata ttatttgaga tattatattt
     6781 atatcatttt caataattgt tatatagcat aaaaaatagt gttaatatta tagaatcggt
     6841 attgattata tttaaaaatg ttatactcta aaaaagtata ttttatttaa aaatttatta
     6901 atacaaagga gatttactat gaaaaataaa ttagtttaca tatgtattat gagtctattg
     6961 ctatttgtag taggatgttc taataatcca aataaggaaa gtaccattaa aaaagactct
     7021 aagaataacc aaagccaagt taaagaagaa aaaagagaac ctgtgaaaaa agatataact
     7081 ataagtttcg ctggtgatgt aactatgggg aactataaag gttcatcata ttatggcagt
     7141 tttgaccatg aatttaaaag acaaggagaa gattacaatt attttcttaa aaatgtaaaa
     7201 cctgtatttg aaaaagatga cttaacagtg gttaatttag aaggtccact tactacagcg
     7261 tcaaatgcaa aagaaaaaaa atttgcattt aaaggaaagc ctggatatgt aaatatttta
     7321 aaatctggaa gtgtagaggc agtaagtatt gcaaataatc attcagaaga ttattttgaa
     7381 gaaggaatga aagatacaca gtttatattg gacgagaata aaataaatta ttttggatta
     7441 gggaaagatg ctgttgtaga tgtcaaagga attaaagtgg gtctattagg gtataatgga
     7501 ttatcaactg aatataatga aaaaaattta aaacaaatgg aagatgatat taagtcctta
     7561 aaaaagaaat cggatgttgt tgtagtgtac tttcattggg ggatagaatt agattattat
     7621 ccagataaaa aacaaaaaga ttttgctcat tatgctatag acaagggagc agatttagtt
     7681 atggggtctc acccacatgt gattcaagga atagaaaagt ataaaaataa gtatatagct
     7741 tatagtctag ggaatttttg ctttggagga aataagaatc cttctgatac agatagttat
     7801 atatatcaac aaacctttac tttttcagat aataaattaa cttcaattaa agagccaaat
     7861 ataataccaa cttcaataac atctagtaac tccagaaata attatcaacc aaaaatattg
     7921 gatggaagtg agaaggatag agttttaaat aaacttgaaa aaataagcaa agatttaaat
     7981 aaataaaatg taaaaaaaaa taaatatata tatttatttt aataatttat attttgttta
     8041 gggtatggta aatcgtggta ttaaataagt agattttaat agttaaatta tagtcaatta
     8101 taataaagct atttaatcta cttttttaat ataaaaatat aagggggaat ttgtaatggc
     8161 ttgtaacact tgtaaaatgt gcgaaagtgc agacaagaaa ttagaaagtt ttgtagcttc
     8221 aaaggatgtt gaaacagcat ttcatagaac agaagatcag aaagttaagt gtggttttgg
     8281 acttcagggg gtttgttgta gactctgttc aaatggacca tgtagagtaa ctcctaaatc
     8341 accaagaggt atatgtggag cagatgcaga cactatagta gcaagaaact ttttaagagc
     8401 agtagcatca ggagcagctt gttatttaca tgtggtagaa aatacagcta agaatttaaa
     8461 aaatgttgga ataactaaag gtgttgttaa aggagaaaaa acattaaatc aattagcaga
     8521 gatgtttgga atagaaagtg atgaaaagta tgataaatgt ataaaggttg cagacaaagt
     8581 tatcaacgat ttgtataaat caagagatga taaaatggaa ttagtagaaa aaatagctta
     8641 tgcacctaga gtgaaaaagt ggaaagagtt aggtataatg cctggtggtg caaagtcaga
     8701 agtttttgat gcaatagtaa aatcatcaac aaatttaaat agtgacccag tagacatgct
     8761 agttaattgt ttgaacttag gtatatcaac aggattatat ggtcttacac taactaatct
     8821 tttaaatgat gttatgttgg gagaaccagt tataagaatg gctcctgtag gatttaatgt
     8881 aatagaccca gactacataa atataatgat aacaggccat caacattcaa catttgcaaa
     8941 tttccaagat agattaaaag atgaagatgt tataaaatta gctcaatctg ttggtgcaaa
     9001 aggctttaag ctagtaggat gtacatgtgt tggtcaagat ttacaattaa gaggagaaca
     9061 ttatcaagaa gtatttgctg gacatgcagg taacaatttt acaagtgagg ctgttttatc
     9121 aactggagca atagatatag ttctttcaga atttaactgt actataccag ggcttgaacc
     9181 aatagcagat aaatataaag taaaaatggt ttgtttagat gatgttgcta aaaaagcgaa
     9241 tgcagaatac ataggtttag acagaagtaa attagatgaa cttagtaaca ctttaataga
     9301 aaaagcattg gagtcttata aggagagaag aggaagtatt gaaatagata taccaaaaga
     9361 ccatggattt gaacaatctt taactggtgt aagtgaaaag aacctgaaag cgtttttagg
     9421 agattcatgg aaacctctta ttaatttaat tgcagaagga aaaattaaag gtgttgcagc
     9481 tgttgtagga tgttcaaata tgacagcagg aggacatgat gtaaatactg ttgaacttac
     9541 taaagaattg attaaaaaag atataatagt actatctgca ggatgttcta ctggaggatt
     9601 agaaaatgtt ggtcttatgt cacctggagc agaagaatta gcaggagaaa acttaaaaga
     9661 agtatgtaag actttaggaa taccaccagt attaaacttt ggaccatgtc tagctatagg
     9721 aagactagaa atagtagcaa cagaattagc agctgaactt ggaattgacc taccacaatt
     9781 accattagtg ctttcagctc cacaatggtt ggaagaacaa gcattagctg atggagcgtt
     9841 tggtcttgcc cttggcttac ctcttcattt agctttacct ccatttataa caggaggaaa
     9901 attggtgaca gaagtattaa cagaaaaatt aaaagactta acaggaggac atgttatagt
     9961 gaatccagac cctaaatctt cagctaatca attagaagaa ataataatag atagaagaaa
    10021 aaatttaggg ttaaaggatg tggaatgcaa tgcatagaat ttttataaat aaagatttat
    10081 gtacaggatg taagagctgt gtactagcat gtatgctaaa acacaataaa gattatgaca
    10141 tgtacactct tgatttagaa aatatagata atgatagtag agggcatata gaattagata
    10201 gtaaacataa taatccagtt ccaattcttt gtagacactg tgatgaacct gaatgtgtat
    10261 tagcatgtat gagtggtgca atgcataaag atagtgaaag tggaatcgtt tcatatgatg
    10321 aagagaaatg tggttcatgt tatatgtgtg taatgtcttg tccatatgga ttattaaagc
    10381 cagatgatag aagcaagcag aacatactta aatgtgattt atgtaaagat gaggagtatc
    10441 ctagatgtgt agctaattgc ccaagtggag caattgaatt acagaaggag gaaaatgatg
    10501 aattatgtag tgttaggggc tagtgcagct ggtataaatg cagtaaaaac attaagagaa
    10561 ttagataaag attcaaatat agtagtaatt tctaaagatg aaaacgtata ttcaagatgt
    10621 atgttgcatc acgttatatc tgaacacaga attttaaaac aaataaattt tgttgatgaa
    10681 gattttatgg agcaaaataa tgtaaaatgg attgcaggta agactgttaa aggtatagac
    10741 ataaataaaa aggtagttca gacagaagac attactgtaa attatgataa acttttaata
    10801 gctacaggtg catcttctgc tattcctcct ataaaaaatt taagagatgg aaattttgta
    10861 tattctgtga gaaacataga tgatatttat aaaataaaag aaaaagcaca aaattctaaa
    10921 aatgtagtta taataggtgc tggtttagtt ggtatagatg cattagtggg attatttaaa
    10981 tatgagcagt taaatatttc tgtagctttt atggaaaagt acattttaga tagacaattg
    11041 gatgaatata cagcatcagt gtatgaaaat aagttcaaag aaaaaggtgt aaaattttac
    11101 ccaagtgcat caatacaaga aattgtattg gataacagta aaaatgttac aggtgttgca
    11161 ttttcaaatg gtgaagtttt agatgcagac atggtaattg ttgctacagg ggttaaacca
    11221 aatgctgact ttttagatgg aacaggaata gaatatgata gaggtataat aatagatgat
    11281 atgtgccaaa caacacaaaa agacatatat gcagcaggag atgttgtagg taaaaatgct
    11341 atttggcctc tggcagtaaa gcaaggaatt gtagctgcct ataatatggt tggaaaagat
    11401 aagaagatag aagatgaatt tgcttttaaa aacagtatga atttcatgga tataccaact
    11461 atttctatag gaatgaatac acctgtagat gatagctata aagtgctaac tagacatgga
    11521 attaatgatt ataaaaagtt tgtttttaaa gataatgtaa tctatggagc agttatacaa
    11581 ggagatattt catatgtagg tgtattaact tatttaataa aaaataaagt tgaaatatat
    11641 gatttagaaa ataggatatt tgatatatgt tatgcagact tttttaatat aaaagaaaat
    11701 ggagaatttt gttatagtgt atagtatata cgtggagtaa ggtttataaa tacagtaaat
    11761 caataaatag agttattcta aaataagaaa aatgacaaat cttatttaga aatagctcta
    11821 ttttttgtaa ttaaatttaa attatattca atttgtaaaa tattattttg aacaattata
    11881 catatactct ctagttagag gtaaatcatt ttttatacct ttagtgaaaa gtatttgatg
    11941 tatatctata ataccagtgt tgaaagcagc agcacaagaa caaaggtata aatcccacat
    12001 tcttataaac ttatcatcaa acatttcttt aatctttgga agattctcag agaaattttt
    12061 ttgccagtat agtaaggttt ttacataatg taatcttaga ctttctacgt ctatagtatg
    12121 atatttgtaa tcactacaaa tatgtatcat ttctcttagg ctaggaataa ctcctcctgg
    12181 gaaaatatat ttttttatcc aagggtctcc ataagattcc tgcaatccac ttatatagtg
    12241 aagtaaaaac aatccaccat cttttaaaac agcatttacg tttttaatga aaagttcata
    12301 gttttctcta ccaacatgtt caagcatccc aacgcttaca attctatcaa acataaggtc
    12361 tgattttttt aattctctat agtccataag tcttacttca agatagtctt gaaggttctc
    12421 ttctttaatt cttgcgttaa attccttaaa ttgctcttcg ctaagtgtaa ttccaacgcc
    12481 gtgaatttta tatttttttg cagcttctat tagtaaaaat ccccaaccac aaccaatatc
    12541 gagaaggctc ataccttcct ctaaatttag tttagttaat atgtggtgaa ctttattgac
    12601 ttgagcatca taaagagtat cattatcatt tctaaagtat gcacaagaat aactaagtgt
    12661 cttatctaac cataagctat aaaaatcgtt gccaatgtca tagtgggcat gtatttcttt
    12721 cttttgatgt ctcattgaat ttgatgagtg caatagatgt tttacagctg aattatcaat
    12781 attgaatttg tctatttgtt ttaaaagtag atttaaagtt aagaataaat ctccctcaac
    12841 ttcaatgtct ctatttatgt aggcttctcc aagtgctaaa gatgtacttt ttaatagttc
    12901 agacttgcta atatcattat ttactataac tttgaactga ggggagccct ctccaattag
    12961 aaattcttcc tcatctttaa gcctaactag aaatggtgtt tcaccaaatt tctctaaata
    13021 gtcttttaaa aaattttttg taatattcat attaccagct ccttctaatg taaatccagt
    13081 aagtttccct gcttaaaata tattatagtc ctttttttaa aaaagtaaaa ttttttcaat
    13141 aaatttttta tttaaaggaa atttattttt tataaagaaa tttcatgttg tagtatattt
    13201 tttgcttata aattttggta ctggatataa tattaaactg ttaatgatat aataaattta
    13261 atctataaag taaataaaat tttgtaataa atagttaaag ctattttata aggagatata
    13321 tgatggaagt atatgctttg gttgggtcaa gtggcacagg gaagagcttt aaggctttag
    13381 agttcgcata tgaaaatgat atagaatata taattgatga tgggatttta atatacaaaa
    13441 acaaggtttt agctgggata tcggcaaaac aagcaaatac tactatagaa gcagttaaaa
    13501 gggctatttt ttataatctg gagcatagac aagaagttag agaagcaatt aaaaaagaaa
    13561 atataagaaa aattcttatt attgggacat caaaaaaaat ggttaatcaa attgtagaga
    13621 gattgtcaat tggaagggtt tgcaagttta taaatataca agatataagt actaaatatg
    13681 aaatagaaat agcaaaacaa gctagaaaag aaggaaatca tgtaattcct gttccagcag
    13741 ttgagataaa aagtatggca agtggtctta gtataaattc tttgaagaga ctttttagaa
    13801 aaggaagtaa taggaatatg gtagttttag aaaaaacaat tataagacca acttttagtt
    13861 atataggaaa gttttatatt agtgctgatg taattaaaca gattattgaa tatgaagtat
    13921 acaactttgg aagcattgat agaataaata aaatcaatat agagaattgc aataatttta
    13981 tgaatatttt tgtaagtata aatatcaatg atttagaaac tataaaaaaa attgaagaca
    14041 tacaacagac aataaaaaag tctattgaga aaatgacttt agttaatgta caaaaaatag
    14101 atattaacat tcataaaatt aaaaaaataa acttcctcca atgttaaaaa ataaactgag
    14161 aaaatgatat actaatttgc aataaagtgt gctataatta aactgtaaat ttaggaaatg
    14221 gttttttcta aatggagtat acataattat aacgtattta taagggggct ttcaaatgtc
    14281 aggaaaagat gtaaatgtct tcgagatggc gcaatctcaa gtaaaaaatg catgtgataa
    14341 attaggtatg gaaccagcag tttatgaatt attaaaagaa cctatgagag ttatagaggt
    14401 ttcaattcca gtaaaaatgg atgatggttc tataaaaact tttaaaggat ttagatcaca
    14461 acataatgat gcagtagggc caacaaaagg tggaataaga ttccatcaaa atgtttcaag
    14521 agacgaagta aaagctttat ctatatggat gactttcaag tgttctgtaa caggtatacc
    14581 atatggtgga ggtaaaggtg gaataatagt agatccatca actttatctc aaggtgaatt
    14641 agaaagatta agtagaggat acatagatgg aatatataaa ttaataggtg agaaagttga
    14701 cgttcctgca ccagatgtaa atactaatgg tcaaataatg tcttggatgg ttgatgagta
    14761 caataaatta actggacaaa gttctatagg tgttataact ggtaagccag ttgaatttgg
    14821 tggttcttta ggaagaacag ctgctactgg atttggtgtt gctgttactg caagagaagc
    14881 tgctgctaaa ttaggaatag atatgaaaaa agctaaaata gctgttcaag gtataggaaa
    14941 cgttggttct tatacagttc ttaactgtga aaaacttggt ggtactgttg tagctatggc
    15001 tgaatggtgt aaatcagaag gttcttatgc tatatacaat gaaaatggtt tagatggtca
    15061 agctatgtta gattatatga aagaacatgg taacttatta aacttcccag gagctaagag
    15121 aatatcttta gaagagttct gggcttcaga tgttgatata gtaataccag ctgcattaga
    15181 aaactctata actaaagaag ttgctgaatc tataaaagct aaattagttt gtgaggctgc
    15241 taatggacca actactccag aggctgatga agtatttgct gaaagaggaa tagttcttac
    15301 tccagatata ttaactaatg ctggtggagt tacagtttct tacttcgagt gggtacaaaa
    15361 cttatatgga tactactggt cagaagaaga agtagagcaa aaagaagaaa tagctatggt
    15421 taaagcattt gaatctattt ggaaaatcaa agaagagtac aatgttacaa tgagagaagc
    15481 tgcttacatg cattcaatta agaaagttgc tgaagctatg aaattaagag gatggtacta
    15541 agatttactc ttaataaata ataggatata attatttaag atataaaggg gctgtacata
    15601 gtacagccct taaattatgt ggcttacaat gaagtaagct atttttgttt taacttataa
    15661 aaaacttgat aaattatgta atttagaatg tattttttac caccacatga ttacaaatta
    15721 gaagagttag attagatttt tagacatgaa cttatgcatt ttaaaagcaa agaccttaat
    15781 ttaaagtatc tagtattatt tttaaaaata gtatattggt ttaatccatt tatatatata
    15841 atggaaaaat atatggattt ggattgtgaa ctttattgtg atgagagagt tttaaaaaat
    15901 tgtactgtac aacaaaagca agaatatgct ctaactattg taaattcaat gaaaagaagt
    15961 acaaattttt caaataaatt tgtagctgga ttaaataaac aatctgatat tagaaaaaga
    16021 gtaataaaca tgttcagtga aaaacataaa aatggtatct taagggcttt gattataagc
    16081 ttgttttctt ctattacatt ttttaaattc gattcaacta gtattattga agagaatgat
    16141 ttcttagaga ggtcaagata tacgtttgaa ggaaggcaag aagacatgtt tgtaacaata
    16201 gattttatat atgcagatgt acctataaaa cttagaaaag agcatgaaga aaactgtaga
    16261 gtcaatggaa tagtaccaaa agattcagat tcaatagaaa ttattcctag attttataaa
    16321 gattgtatgg atttaaaaaa ataagttaga cttaatattg atgttgatat tatgaatttt
    16381 ttatatttaa gcaattgaaa attttattat agattcaatt ttatatgtgt ttaagtatga
    16441 aatttataga tagttgaaat aattaaatat gattgatgca taggaatcat tactaagcta
    16501 taattaatta taagggagtt taaataaatt atgagttaca caattataaa atgataaaaa
    16561 atagttatct gttacaaaaa atgtatcttt ttaattaata aagataatat gaaaacttaa
    16621 aaggcaaata aacttaaaca taaaatactc ttaaataaga taggagaaaa atgtatgtct
    16681 aactttattc taaatgggat ttttagaaca gttatagtaa gtagtctaag cattcttatg
    16741 atattgattt tcaagaaaaa tgtatttaaa agatttagca aaaaatttaa ttattatata
    16801 tggatgatag ttgttattaa attattttta ccatttacat attatacttt tacaataaat
    16861 atcttgagaa gtaaaaaaca cattaatatt aataacataa atttagaggg atttaataat
    16921 gtttcaacta taagtaataa tattatattg tacacttgga ttataactgt agtagtttat
    16981 ttaatttaca ctatatttaa atatataaaa cttaaaaatt taataaatga cttatcttat
    17041 gatgttgatg atggagagat tgtaaatctt tataaaaata tattagaaga atttaatata
    17101 acaaaagata taaaactcaa atactcttat gaagttgaaa caccagcatt ttttaattca
    17161 tgtgtgcttt tgccaccaca tgagtataaa ttgaaagagt tagactggat ttttagacat
    17221 gagcttatgc attttaagag tagggacctt tatttaaaat atgtaatcct gtttttaaaa
    17281 acagtatatt ggtttaatcc atttatatat ataatggata aacatataga tttagattgt
    17341 gaactttatt gtgatgaaag agttttaaaa aatcgaaatt cagaggaaaa aaaggattat
    17401 gctttaacaa ttctaaatgc aatgagaaaa ggttcaaata cttcaaataa atttatagct
    17461 ggactccata agcaatctga tattaaaaag agagttatga atatgtttaa tgaaaagtac
    17521 aagaatggta tattaatggc attaacttta tgtttattat cttctattac ttttttgaag
    17581 gttaattcaa cgagtattgt taatcttaat atattgccaa aaggaatgga agaaaaagat
    17641 gttactaata gtgcaattgt tacaatgata gaatttacat atgcagaagc tcctaaaagt
    17701 atgagagaag aacataaaat aaattgtgaa ttcttaggtt taatacctaa agactctgat
    17761 aaaataggtg ttggtataga atattatgac agtctattga atgattaaat aaatattatt
    17821 ttaacagata ttaattataa agttttgaat agtaatttgt tgagaaataa atatatcctt
    17881 ataaaaatag atacttatat accaagaata aaaaatagtc aagatagtac acaaggatat
    17941 acttataaag aactcaaata agtgaaataa attattaatt ataaggttta taattttaaa
    18001 aaattagata aattcaggtg taaaatacta taaaacacaa ttcggaaaag tgtatgttga
    18061 acttcattta aaatggaatt tcaaagttgc tatagtaagt agtttaagca ttcttcttat
    18121 attaatcttt gagaataatg tatttaaaaa agttagtaaa aaatttaact attgtatatt
    18181 aatgatagtt gctattgata tattatctaa aaaaattaag tatgaaaaga ttactgctag
    18241 gacaatcttt tcatacttaa tttttaatat ttatattatc caaaattttc atctttttaa
    18301 cttagaattt gagaatcact attaggaaat aggctttgat ttatgtaggg ttaaaaattt
    18361 cacagaatat ctaactttat actaaatcca tcaaaaaata tacgccatat aaaagcgcat
    18421 aaaaaaacac ataaaacaat atttatgcgc actttaacct cgaatggtac aaaattcttt
    18481 acttttacag acccatatta tataattaag taaataatgt atatttaggt gacatattgg
    18541 ataagctaat ttatttattt tattaatata atgatactat aaaagttata taaagtggtt
    18601 tacaaaaaag ttacagatag agcttttatt aatgtattgt gtattagtat tttatcctta
    18661 ggtacacttc acctacaaaa tagaatcata agataattat gggaaaattt taggaggttt
    18721 atcgtgaaga aaaagatatt aataccagta tttgcatcag ttatggcatt atcagtaagt
    18781 tcaattgtaa atgcagatga agtaaatgat tcatctcaaa ataaggatga taaaacaaat
    18841 actgaattaa atttaggtga atataaagaa gtaaaatata aagttgcaaa aataaaagat
    18901 ggagtagcta taaagataag agaagaaggt caagttcaaa acatagctta ttctggagat
    18961 gaatttacgg ttttaggaac tcaaggagaa tgggttaagg ttaaagttga agatggcgaa
    19021 ggttggttag ctactagata tgtagatata tctgaaggtg taggatatac taacgctgat
    19081 aaagtaaact taagaaaaga taagagtgaa agttcagaag taattgaaga gctggaaaaa
    19141 ggaagttctt tattggtttt ggaagataat ggagattggt taaaagttaa agatggggaa
    19201 acagaaggat atgtaaaatc atcttatata tctgataaag ctcctgtaat agaagagcca
    19261 caagtaaacc caagtattga ccaaaatgta gattctacaa ataataatca acaaaacaat
    19321 gcaaatacta atcaacaaaa taataattca aatgtgccaa cagctaactc taatgcagtt
    19381 caagctgtac ttaacttggc atattctaag caaggttgtc catatgtttg gggagcagaa
    19441 ggaccaaaca cttttgactg ttctggattt acacaatatg tgtatagaaa tgcagtagga
    19501 aagaatatac caagaacatc taaagcacaa tctaaatatg gacaaactgt aagtaaagca
    19561 aatttacaac caggagactt agtattcttc acaactaatg gttctggttc tgttagtcat
    19621 gttggaatat atgtaggtgg gggtaatatg atacattcac catcaacagg aaaaacagtt
    19681 agtgtaacta gtataaattc atcatactat acagcaagat ttgttacagc aaaaagaata
    19741 ttataatcgg tttgtaatat gacaaattga taaatacata aatatatata aacgctctat
    19801 attatattta atttaatcat ggaaacaaaa aatgattata ttttaaatgt actatagagc
    19861 gtttttgtat cactttaaat ctaatcaaga ttcaagttaa tatgactgtt aaataggagc
    19921 cttttcatat gatgattatt tgcgcaatcc cttgacagga tagggcaaag gtggtaagct
    19981 actaacagta caaaaatact tttaatttta gttcagagag gctaaaaaag gaggacgaaa
    20041 gatgttaaaa tcaagaaatt taatccagcc agaagacttt tcaatagaag aaatagatga
    20101 aatattagaa ttagctcaaa aaataattga caatccgtca aaatattctc gtatatgtga
    20161 aggaaaacta ttagctacat tattttatga accatcaaca agaacaagat taagctttga
    20221 atctgctatg aacagattag gaggacgtgt agtaggcttt tcagagccaa attcatcctc
    20281 tgcatcaaaa ggtgaaactc taggagatac aatgaggata gtatctggtt atgttgatat
    20341 aatagctatg agacatcctc aatcaggtgc agcttcagaa gcagctagat atacggaagt
    20401 gccatttata aatgcaggag acggcaaaaa ccagcatcca actcaaacac ttacagactt
    20461 acttactata aaatcattaa aaggaacttt agaaagccat acaataggat tatgtggtga
    20521 tttaaaatat ggaagaactg tgcattcttt ggttaaggct atggcaagat ataaaaatac
    20581 aaagtttgta tttatagcgc ctgaagagtt aaaaatgcca gattatataa aagaagctat
    20641 aaagggacat gcatattatg aaactaataa tttggatgat gttataggaa gcttggatgt
    20701 tttatatatg actagagttc aacaagaaag atttgaagat aaatcagagt atgaaagact
    20761 taaaaactat tatattttaa ataaagctaa attagaaaag gcatctaaag atatgttagt
    20821 aatgcatcca ttaccaagag taaatgaaat agacatagat gtagatagtg atgatagagc
    20881 tgtatatttt aaacaagcta aatatggaat gtatgttaga atggctttaa taataaaact
    20941 tttaggtata aatgaagact aatagttatc atataatagt aaaacaaagg agattttggg
    21001 atgtataaaa ttctggaaaa tatatatata ggcgaagata tgtatagaat gaaagtaaaa
    21061 ggtaattttg aaggtaaaat gggacaattc tatatgttaa gagcatggga tacttatcca
    21121 gtactttcaa gacctataag tatacatgat atagatgaag aagggataac ttttctttat
    21181 aaagtagtag gagaaggaac tcaaattttg agtaacctaa aggtaaatga taatataaaa
    21241 ttagaaggcc cttatggtaa tggatatgct aaggttgatg gaaaggtagc tctagtagga
    21301 ggaggaatcg gtgtagctcc actatatttg gttgctaaaa atataaaaaa ttgtgatgca
    21361 tatcttgggt ttagagaaga tgttatatta gaagatgaat ataaacaagt atgtaataaa
    21421 gtctatacaa cagtaggaaa tacttttgta acggatataa ttgatgttga gaaatatgat
    21481 tatatattaa catgtggtcc aactccaatg atggaaaaat tagttaagat ggtagaaggt
    21541 actaaaacga gaattatggt atctcttgaa aaccatatgg cttgtggagt aggtgcctgt
    21601 ttagtatgta cgtgtaaaac taatggagga aataaaaaga cttgtaaaga tggtccagtg
    21661 ttctggggag aggacgtgat ttttaatggg taatttaagt gtgaaatttg gaagtgtaga
    21721 atttaaaaat ccagtaataa tggcgtctgg aacttttgga tttggaaaag aatataatga
    21781 gatttatgat atacaaaaac ttggtggtat aagtagcaag ggtcttactt taaataaaaa
    21841 acctggtaac aatggtatga gagttcatga gacatcatcc ggtatgatga atagtgttgg
    21901 gcttgaaaac cctggagttc aaggatttat agactatgaa ttaccttttt ttagtaaact
    21961 agacttagtc agaatagcta atgttggagg tgggacatta gaagactact tattaggggt
    22021 tcaaatgtta aatgacaagc ctatagacat tattgaatta aatatatctt gcccaaatgt
    22081 caaagctggt ggaatggctt ttggtataaa aaatgaagtg gcaagagaag ttgttcgtga
    22141 ggtaagaaac ataacgaaac tgcctttagt aataaaatta tcacccaatg cagaagatat
    22201 tgtaggaatg gcaaaagttt gtgaagaaga aggtgctgat ggagtttcat tagttaatac
    22261 ttttaaagca atggcaattg atataaaaaa tagaagacca gtatttgaaa atgtatatgc
    22321 tgggttatct ggaccagcta taaaacctat agctttgaga atggtacatg aagtgtgtaa
    22381 aaatgtaaat atacctgtaa tgggaatggg cggaataact aaagccacag atgctataga
    22441 gtttataatg gcaggagcta cttgtatcca agttggaact gcaaatttta tgaatcctag
    22501 aataggtatt gaaataatag atggaattaa tgaatttatg gatagagaag gaataaaaag
    22561 tttagacgaa ataagaggaa ttatataagg tagaataaat agtattttaa agataaaaaa
    22621 tatagattaa ataaacaata attttataac attaacatgg aggaaaaata atatgagtaa
    22681 agtagatgta gtggatatat taaagaaaag tgatgcattg ttggagggac attttttatt
    22741 atcttcagga aaacacagta atagatacgt acaatgtgca aaagtattaa gatttccaga
    22801 atatgctgct gaggtattaa gtacagttgt tgaacaaata aaagacttag atatagactt
    22861 agtagtagga ccagctatgg gtggagtaat agtttcttat gagttaggaa gacaattagg
    22921 aaaagaagct gtatttactg agagaaaaga caatacaatg gagttaagaa gaggatttga
    22981 agttaaaaaa ggagcaaaga taataattgc tgaagacgtt gtaactactg gtaaatcaac
    23041 tatggagaca aaaagagtat tagaagcctt aggtggagaa gttgtaggtg ttgcatgtat
    23101 agcagataga actaatcatg atataggtat gcctatatat agtgctataa aacttgatat
    23161 tcaagtttat gaatctgatg agtgtccttt atgtaaggaa ggaaaattac cagttgttaa
    23221 gcctggaagt agagagttca aggaattagg gatgtaataa aaacttaatt atttatagtg
    23281 ttacttaaaa aatgtaaatt ttttagagta aaaatttgta atataacata taacttaaaa
    23341 ataaaaagca gttagcctta taaatgaagg ttagctgttt ttttgttaaa aatatattta
    23401 caaaataaga gatagtagca gtacaatata agtataaggt aaaagtatta tatattaggg
    23461 aggggtaagt atgaaaaatc aattaaatga aaatcataaa aaggttgtat tttttggagt
    23521 aggtgctgta ggagctactt ttgcagaaca attttttaac tctaaatatg attttaaaat
    23581 tctttgtgat aatgaaagaa agaaaagata tttagaagaa ggatttataa taaatggaaa
    23641 aaggtatgat tttgattatg taactaaaga tgagtataaa caagaggctg attttataat
    23701 tataggtcta aaatataata atttaaaaga aaatataaaa gaattagatg gattagttgg
    23761 aaaaaataca gttataatgt ctctgctaaa tggagttgat agcgaagaga taataggaga
    23821 aagatttgga attgaaaaaa tggtatattc atatgttacc aatatagatg caaagaaaat
    23881 caataataat attatacaca ctactaatgg gataattgta tttggtaaca aagataatag
    23941 tgaagataga aaaactaata taataaccga agtctttgat gatgtaaata tagaatatac
    24001 tttatcaaaa gatattcaac gagatatgtg gtggaagtac atggttaata ttggtgtaaa
    24061 tcaaacttca gctatacttg gtgcacctta tggagttttt cagagttctg agcatttaag
    24121 agaattagca aaatctgcaa tgagggaagt tgttgctata gcacaagcaa aagacatatc
    24181 tcttacagaa gatgatgtgg aacattcatt acatagaata ctagaacatt caaaagaagg
    24241 aagaacatca atgcttcaag atgtggaagc tcatagactt acagaagtag atatgttttc
    24301 taagaatatc tgcaaacttg gaaaaaaata taacatacct actcctataa atcagacttt
    24361 cttttatatg ataaaagtaa ttgaaagtag attttaaagg gtctttgtaa gaaatattga
    24421 taattattga ttttaaattt tattaatgtt atatactatt taagttacta aatattttta
    24481 actcttcaat gaaaatagtt gctgcttttg ataaagttat atcttttgga aatatatatg
    24541 agaaatgtct gttgtagctg cttccaagct ctatgacaga cagctcattg tttagaacag
    24601 gtaagcttgt cactagatta gagactattg taattcctaa attatttcta acagattctt
    24661 tgacagcata attacttcca aaaaccatca tactttttgg aataatttct ttaacactta
    24721 aaaacatatc taaataatct ctggttccag aaccatcctc cctgactatc catttttggt
    24781 tttgaagttt atcaaaacta aaatctttta ataaatgact cttgtatgga agtgctaaaa
    24841 ccatcttatc ttcaaaaaaa tattcttgaa taaatgaagg ggaagagcaa gtgccttcaa
    24901 taagaccaat gtctaaaata taatccttaa catgagaaga tacgattgat gtatttttta
    24961 taaaaacttc tacatctatg tcaggatact ttttagaaaa taatgctaga aaatttggta
    25021 aaatatactc tccaatagtt aaacttgcac caatctttaa gtgaccagtt atagcatctg
    25081 aaacttggct tacatcatga taagtaatat ttaaaagatt taatatttct ttagctcttt
    25141 tgtacaagat ttgtccactt tctgttataa aaatggtttt ttgtttaata gacctgttta
    25201 taagcacaac tttaaaataa ttttctagat tttttatgtg tttacttaca cttggttgtg
    25261 atagatttaa atattcccca gctttagtaa agtttttgta ttcaacaaca gctacaaatg
    25321 tttttaattc ttcaaacaat tttttgtctc ctttacctaa tgaaatttta cattttttat
    25381 atactttcta cagataatta tactatatat aataaccatt aaaaacatta atcattacga
    25441 tattatatat ttattttact tatggttatt acaattatat actattgcta tgaagaaaac
    25501 tatattaatt ataaggtagg tatattatgt taaagaaaaa aagcagtttg acattaaaaa
    25561 ctataaggga aatattacca ggactgtttg tatcagtatt agtaggttat attagtatat
    25621 ttatttctat tttaattcct aaggtagggg cagcatcaat ttctatattt ttaggtatgt
    25681 ttgtaggaaa cctattttta aatcaaaagg tatttcaaaa aggttataaa ttttcagaga
    25741 cagatttgtt atcatattca atagtactat taggagcaac actcagtgta tctaccttaa
    25801 tagacttaaa agtttcagga attttattta taatattgca aatgacaata acaataatag
    25861 ctgcacttta tataggtaaa aaactaggat ttgaagaaaa ctttagattt ctaatggcaa
    25921 gtggaaatgc agtatgtggt tcttctgcaa taggagcaac agttccagtg gttaatgcga
    25981 cagataaaga aaagggaatt gcagtaacta ttgtaaatgt tactggtata tttcttatgt
    26041 ttttacttcc aataatatct caatttttat atagccatga acttgtaaaa acttcagcaa
    26101 tgataggtgg aactttgcaa tcaattggtc aggttgtagc aagtggagca attgttggag
    26161 aaaatgtaaa agatttggct actattttta agatagtaag agtaatattt ctagttgttg
    26221 ttgtattagt atttggacat atgaaaaatc aatctaatag ggaaattctt gaagaagaaa
    26281 aggatgagat aaaaaagaaa aaagtgacta taccatggta tgtaattgga ttctttataa
    26341 cttgtgcact tttttcaatg aacattatac caaaagaagt atctgtgttg tgcaaagaaa
    26401 taagtaacaa actagaaata atagctttag cagcaattgg attaaaggtt aatgtaaaag
    26461 atttagtgaa acaagggaaa gaagtttctt tatatggttt atttgtaggt atagtacaag
    26521 ttgtatcagc tgtagtttta ataaaaatat ttatatagca taaaaatagt atttgtaata
    26581 ttaatttgat taactaaaat aatatgatac taataattta taatataaat aaaaaatgaa
    26641 tttcga
//
LOCUS       DAMJZE010000032        26147 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJZE010000032 DAMJZE010000000
VERSION     DAMJZE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.032"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(101..1387)
                     /locus_tag="PC541_001279"
     CDS             complement(101..1387)
                     /locus_tag="PC541_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2838750.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1510..4608)
                     /locus_tag="PC541_001280"
     CDS             complement(1510..4608)
                     /locus_tag="PC541_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2838751.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5334..5492
                     /locus_tag="PC541_001281"
     CDS             5334..5492
                     /locus_tag="PC541_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838752.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(5779..6483)
                     /locus_tag="PC541_001282"
     CDS             complement(5779..6483)
                     /locus_tag="PC541_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838753.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6474..8081)
                     /locus_tag="PC541_001283"
     CDS             complement(6474..8081)
                     /locus_tag="PC541_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2838754.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8335..9513)
                     /locus_tag="PC541_001284"
     CDS             complement(8335..9513)
                     /locus_tag="PC541_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838755.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9739..10233)
                     /locus_tag="PC541_001285"
     CDS             complement(9739..10233)
                     /locus_tag="PC541_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2838756.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10234..10995)
                     /locus_tag="PC541_001286"
     CDS             complement(10234..10995)
                     /locus_tag="PC541_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2838757.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            11697..14531
                     /locus_tag="PC541_001287"
     CDS             11697..14531
                     /locus_tag="PC541_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2838758.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            14574..15818
                     /locus_tag="PC541_001288"
     CDS             14574..15818
                     /locus_tag="PC541_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2838759.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(16233..17975)
                     /locus_tag="PC541_001289"
     CDS             complement(16233..17975)
                     /locus_tag="PC541_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838760.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(17959..18633)
                     /locus_tag="PC541_001290"
     CDS             complement(17959..18633)
                     /locus_tag="PC541_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838761.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(18670..19659)
                     /locus_tag="PC541_001291"
     CDS             complement(18670..19659)
                     /locus_tag="PC541_001291"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2838762.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(19707..20951)
                     /locus_tag="PC541_001292"
     CDS             complement(19707..20951)
                     /locus_tag="PC541_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2838763.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(20993..23539)
                     /locus_tag="PC541_001293"
     CDS             complement(20993..23539)
                     /locus_tag="PC541_001293"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838764.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(23747..23908)
                     /locus_tag="PC541_001294"
     CDS             complement(23747..23908)
                     /locus_tag="PC541_001294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838765.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(23964..24980)
                     /locus_tag="PC541_001295"
     CDS             complement(23964..24980)
                     /locus_tag="PC541_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2838766.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(24977..26047)
                     /locus_tag="PC541_001296"
     CDS             complement(24977..26047)
                     /locus_tag="PC541_001296"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2838767.1"
                     /translation="MNKNQIFKSNNLNFDMLSNGVAIYFCLTNRCNLFCKHCCVESSS
                     NKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPDTKLILS
                     TNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVNLLKDNGFS
                     NIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPIT
                     IPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFD
                     NEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELEL
                     DRWCSMMKHNLNTAVWGDEKVN"
BASE COUNT         8966 a         4291 c         2677 g        10213 t
ORIGIN      
        1 tttgttctaa tttttttcat aataagcttt aatactttat atagtaattt gttgtagaat
       61 tttatacaat ataatgattt atacagtttt ataaaatatc ttaaaacagt atttcagtaa
      121 tattttttgg tcttcctcca ccaatactag tctcttttgc acatattcga cctaaacctg
      181 catttactat tttatttaat atacgtcttg cacttctttc acttacattt agatactctg
      241 ctaactcttt tgaatcataa atcttacttt ttctattctc atttatagca ataatttttg
      301 caactgactc acaactcatt ccagtctttt tagatatttc gattaccttt tcatcagata
      361 gcatcaaaga ataatttaat tcattttcgg aagctaaagg tcctcttatg ttttcatctt
      421 catctaccaa atatatacca atttcttttg aatctatgga gtgtcttaat gctttatgtg
      481 cattattttc agcctgatac gcagttactc cagtaccaat tcctatacta agagaaaagc
      541 ccatgtcttt tatctctctc tttagattga atagcttatc ataattatac tcattatcag
      601 ctaccccttt atgaacaaat acaacataat catttcttcc aagtaaaaat aaggaacctt
      661 gaatgcttct tacatagttt actacaagct tatccatatc tgattttttt atcatgtcag
      721 aataatagtt atctatcttc tctttataat ctattaaact caatatttca acagcaattt
      781 gggaaaatct tgccttactt agagcatatt cactctttac cttgtcataa ctttctttga
      841 ttaattgtat agtagcttgt aaacgaaata caggatagcc tctttctttt aattcattat
      901 atactgcacc aaaaccagtc aacattatat ccgtcttatt ttcttcaaat aattttatat
      961 gccattctac aagttcagac tctcctactt ccaaagaaaa tggattacaa aatacactcg
     1021 ttgctttaat atcaaattct ttaagtgcat cttccacaac atatctatca actacatcta
     1081 tactgaattt atctaatttt atatttgcac tttttattgc ccaaaatgcc ttcattatac
     1141 tcgtcccact tctcggtaca aatgaatgtg gcttacttat ctcatactta gattttataa
     1201 attcataaac tgcacatcct gtaaatataa tagcatcaca ttcgtcttca catttagaaa
     1261 taacttctat tgtatctact actttttctc ttacatatat tttagtcttt aaatcactgt
     1321 ctatctgttt taaatcatcc tttatcttaa acccagagtc tgaaggacct ataataccca
     1381 ctatcataaa cttcgctcct aatccataaa tattatactt tttaaaaatt atatcctcac
     1441 tattttctac ttctatttaa ttattttgaa ctactccatc ctacctacat ttagaatgga
     1501 ataattagct tatttttgat aaagtaactt ctcaaaatca gcacttggaa taggcttaca
     1561 ataataataa ccttgaatta ccacacaatc taaagtcact aaaaattcag cttgttcttt
     1621 tgtctctaca ccttctgcca ctacatccat atctaaatct tttgccatgg ctatagtgtg
     1681 ctttaaaact attttttctc tgtctgtgat tccttcctca ttaaagaaac cacaatctat
     1741 ctttaatgta tcaatattaa tattttttaa catatttaat gaagaatatg ctgaaccaaa
     1801 gtcatccata tttaaaacaa taccatccat tctaaggtcg gctattatct tagtaaataa
     1861 ttttatattt ttaataaata aactttctgt aagctctaat tctaaaaaag attttggtat
     1921 actatattta tttattaaac ttaaaacaat ttctttaaaa tgtggattat gcaaatgtaa
     1981 tcttgataca tttacagaga taggaactgg ctcttcacct aaatctatcc attttctaat
     2041 caaagaacat acttgttccc atacatacat atctaatctg ataataaacc cattcttctc
     2101 aaatagtgaa ataaatttaa ttggaggtat tatacctttt tctggatgaa cccatctaac
     2161 caatgcttct gcaccaacaa ttttatttgc attaagatta tacttaggtt gtaaatatac
     2221 atggaattgc ccattttcaa gtgcgtattc catttccctt tccatctgtt tttcttcaag
     2281 tgcatcattt cttatagtat catcataaaa tatatagtta ttgatacttt gtttctttac
     2341 ttttttgtga gcatatcctg ccatttcaca cgcatttgta attggaatat gattatccaa
     2401 aattctataa accccaaaac atggtactaa catatattca aatttttttc tatttaagct
     2461 atttttaatc ttataaatta ccttatgaat atctctatca gagtaactct cataaaaaat
     2521 gcaaaataca tctgcataca tacgacaaca tataccatta gaaccaatgt ttttttgaat
     2581 caactctgca acatatatta aagctttatc tcctcctcta actccataaa tgtcattaat
     2641 taccttgaac ttatctatat ccatcattat aattgcataa cgcttgtcag gatatctttt
     2701 aagcattaaa cttatttgtt cacaaaaact ttcataattg tatagtcctg ttaatgtatc
     2761 tttttggttt ttatgtagtt gcataagata atctatattt tttatagcaa ctaaaatatg
     2821 tgtttgtttc tcacctaact tatttttact actaatcttt aacatataaa ttaatgccca
     2881 atagtacttt ttatctattc ctaaacgtct atactcaaaa tttagttcta aatcgtcttg
     2941 cttgctatta ttaagactat caagagattt tagaaggtgg ttatcattaa ataatattat
     3001 atcatcagga tgaattaaat gtttttttat gtatgaaaaa gttttttcaa gattttcctt
     3061 tataggaaaa ctaaaaatat tgctctctga ttgataaatt ggagtaatta aattcgtatc
     3121 aatgcaaata gataaaatta tatcatatgt attataagct aatttttcat aagccttaga
     3181 ataccctttt atcattaaat catcaaaata aaccttatca actaactctt tatgtttaaa
     3241 taaaatatta ttcgtcgaat cagctaagtc actaattact ttcatttcat tatttctaag
     3301 tttgttctta ttataatacc tcttcttatc ttggtacatt ctctcatcag caactttaat
     3361 tagcttatgt atatcaatat tatcgtcact ccaaatactg cccactgcta cactatatat
     3421 catctcatcc ttaaattctt cacttaacca actcactaat tcttcaaata gttgttttga
     3481 tatatcttca cataatacaa caaactcatc tcctccaata cggtaaacat tataagaatg
     3541 aaagtatttt tttattatta cacatgctga cacaatagca gaatcgccat tagcatgtcc
     3601 aaagttatca ttaatatctt ttaacccatt tatatctaca aatacaactc ctatggattt
     3661 aacttttcct ttttgaaaat tatctatata cttaatatat ttatttctat tatatgcacc
     3721 agtcaagaag tcatagtagc tcatataatt taatttattt tctacttttc tttttttcat
     3781 ctcagttaat aaaaaataag atagggatgt caaaagagag gtatctccaa cagctttatg
     3841 tggattatca actccaataa accctactac tttatcatct tgttttagtg aagatgtcat
     3901 taaactttca attttttgag gttttaaaat ttcatattct attggctgtt cttcttttat
     3961 attttctata tcatcaatta ttacaaaacc tttttgttcg aataataaaa accaattctt
     4021 taattcatta atatcaagat ttgttagtgc ctgtttttgt tcatcaacac cttcattgca
     4081 ccattcatat gtattagtta ctttttttct atcttctgat acttcaaaaa catatgctct
     4141 atcagcttta tgaaatttac caatattggt taatacaaga tttacagcat cagaaaaatt
     4201 agattctgtt ataagatgtt taatacatcc aactaataac tcctttatct ctaatttttc
     4261 tgctattgac ttacttatat cttccttttc tgtaatatca agagctattt ctaatcgtgc
     4321 atgtttgccc tcccatttaa ttaacttgtc ctttaatata aaatgtctat ttgttcgagt
     4381 attcgtattt tcccatatgt aatattcatc ttcttttaaa atagagtttg tacaaaattc
     4441 gcagggagaa tctttcccct gtaatgcttt ataacatttt aaatgggata tatcattaag
     4501 tcccattgcc tttttcccaa tttcattaat atataaaagt tcatatgtat ctaaagaaga
     4561 aacatataca atttcatcca actcatctat taaatttatt acatccattt atatatatct
     4621 ccttttattc attttccaga ttgaattttt aaagctttta aaattttgct atatatttgg
     4681 caatgatatt atatttcttt atgttttaaa atagtacctt aatgaggttc tattttttag
     4741 taaatagaat tattattgtg tcaatcgtac aatagagttt tgtaatacct aatttaatat
     4801 atttataatt aattatctca ttatgtgttt ataattaatt ctcatataaa tgacaaatct
     4861 ccacctcaat taagtattcc aatcatatat gaagataata cttcattcat ttaaaaatat
     4921 aaaactatta tatattaaaa tttcttcaat aataaattat ctagattgtt actttgatat
     4981 attatataat atcacaaagg ctgatataaa aagacaaaaa gggcaatttt tttatatcaa
     5041 aaaaaaatgt taaattgata ttatccatat ataaattgat attatccata tagatgctat
     5101 atttaacaat tctacttact tcccttacac ttattagcat atagttatta agagtttgtt
     5161 gcaaaacaac acttagcaca ctttaaaaag tgtggtctaa taagtaatca acgaagaaga
     5221 cataataaag cataaaatat gatatacttt tccacgccat caaaactgag gattttatta
     5281 ttaatatatg ctaaaatatg cttaataaat taaattttaa tgggagaata gttatgaaaa
     5341 tatttagttt cttgttgttt gtgataggaa tatgtttatg tattgctgga tttataggac
     5401 attacccagt atttgttggt acaacattga gttttcttgc aattgcaatg atagctgtaa
     5461 gacaagtcaa aactaagaaa aaaatgaaat aggcaaatcc taactttaca agttaatatt
     5521 acacaagtaa ttagtctaaa attggatact ttttctatta cagaaataca tcatccactt
     5581 tgtattttct taaaaaaata aaatagctat gtaaattcaa gattagatag attaactctc
     5641 tatccatttc attgtaaaat atacatagct atttgtattc gatttaaaat acttagattt
     5701 tcttacataa caaataattt ctatctgatt attactcata ttcatttagt aaccactcag
     5761 caactaatca tttagtatct attcatccgc catacggtaa cctaccccaa cctcagtcaa
     5821 aatatattct ggctgagcag gattcttttc aattttcctt cttatacttg ccataaaaac
     5881 tctaagcgac tgattttcac tttcatagta atttccccaa actttatcta ttataaactt
     5941 atgggttaag actcttcctg aatacttaga catcaactca ataatcttat attcgattgg
     6001 agttaaatgt atttcttcac cattaattga cacaatatga ttttcataat ctatctttag
     6061 gtttttaact tcaaaagact ttggagcatc attatttata ttatcctgtt gagctttatt
     6121 tctaagagcc acacgtactc tagccaaaag ctctgttaca ctaaaaggtt tagttaaata
     6181 atcgtctgct cctccatcta aagcttccac cttatccctc tcatgctctc tcgctgaaac
     6241 aacaattatt ttattagtct ttgcacatgc cctaactcgt cctattacct caatcccatc
     6301 aatatctggt agtcctaaat ccaaaattat caaatctggg tcaagagaat gtataagtga
     6361 tatagctgtt cctccattat ctgtctctac acacctataa ttttgagttt caagagatac
     6421 ttttataaat ttacaaattg gcttatcatc ttctacaatt agaactaatg gctttatttc
     6481 cacttgtctt tcccctcctc atcatacata ggtatgctaa atctaaatgt agctcctcca
     6541 gaaggattat taaaggcgac aatttctcca ccatgagctt ctactataga tttacatatc
     6601 tccaatccaa gaccaatacc tcttctttca agatttctag acttattctt agtataaaac
     6661 ctattaaaaa tattcccgat atcttcattt ggtattcctt ttccattatc tgcaacttca
     6721 aatagtactt gatttgactc tttttttaca gttagttcta tcttagaatt tttaggtgta
     6781 tgccttatag cattatcaat tagatttact aacacttgtt ctattaataa tacatccaca
     6841 tgaacgatta ttatctcatc tggaatattt gtttttatgt ctctactatt ggcaaacttt
     6901 ttgactctta atatagattc agctattatt tcatcaacaa cttctggtcg tttttcaata
     6961 tccaattttc cttcatctat tctggtcata ctaagtatat tctcaacaga atgagttagc
     7021 caactcgtat cttcatatat atttttcaat aattctactc tagtttcttt atctatcaca
     7081 tcatcatttt caagtattgt tgaagatgaa cctaatatac ttgtaagtgg agttcttaaa
     7141 tcatgagaaa ttgacctaag caagtttcct cttaatctct ctctctctgc ctcaagattt
     7201 gcttttttat ttttctcaaa taaattttct ctatcaattg ccaacgcaac ctgtgttgaa
     7261 actgcttcta atagtatctt ttcattttct gtaataatat ctatcttaaa acacgcaaca
     7321 cctataacac caagaacatt tgattcttga ccttttatag ggaaataata tgcactgctg
     7381 tttttacaat attttgtccc aattccaact gggttacctg tcctaaaagc ttcttttata
     7441 gcttcttttt ctaatactga atggaatata ttctcacttc catcattgtt acaaatataa
     7501 atacttggct tacctagatt attattctca tctgcaatag tcactattac agttctattt
     7561 aatatattta ctaaattaga cccacaaaat tcaataattt gatttttatt ttttgcttta
     7621 agcaacgttt tgttattctc atagagcaat cttatcattt tttctctctt atgagaaagt
     7681 ctcacttctc tttttatcct agaagttaaa gtacttgtta taattgctgc cattagcata
     7741 atacaaaagg tcataggata atcttttcta taagctaaaa aagtatataa tggatgcgta
     7801 aagaaaaaat taaatgatag cactcctatt actgaagaaa gtattccaaa aaaatatcca
     7861 taggttatac ttgtagcaaa tagtacacct aatatgtata ctagtataat attagattca
     7921 ttaaaaccta ccttttcaaa aactactgat attccagtag caattgtcat aaccaatata
     7981 gtttttatga agtccttaat aattatcttt ttactgagat agccatcttc atcataatca
     8041 tactctttta agtcttcatg ttgtagtcgt tcctttttca tttactacaa ttcctcctta
     8101 ttgttactgt ttaaattggc tctattttat ataatacaat tttatcataa taaattcaat
     8161 caatacattt aattaatgta cttaaattac taaaaggtgc tgtcttaaaa tgaaaataaa
     8221 tctctatttc aaaacagcac ctatatattt aaattactaa tttagttttt acatacttaa
     8281 atcaatagtt ttattcaatt tcataaatta taatacttca attataaaac atttctatcc
     8341 aacaagcata aatataaagt gggcacatat acctgcacat aaaccaccta tagtatgcga
     8401 aagtatcgct ttaccagcca atggtcttgc atctaaagca tccatcatac ctatgtgtgt
     8461 acttaaatat ccactccaac acatacccat tgctgtgaat actgctatat cattaggagt
     8521 tatagcgcct gattttataa actctggtac taaagatatt gctgctccaa cagctcctaa
     8581 tgcagttaca gggaatgcta ttgcctctgg tgatgtgaat ccaaataatg gttctattat
     8641 gaagctaatc ttatcaccaa tagcaggtaa taattttata ccttcatatg cagctcctgt
     8701 gtaaacagct tgacctgtag cagggtctgt agatggtcca aaagttaata acataactaa
     8761 tgtacaaact actaaaactc ctggtattat agccataccc atttcaacac ctaacttacc
     8821 accttcaagt atagcatcca atgttctttg gaatacatta ccatctctta tttctctaaa
     8881 ctcttcaagt tttgcacctt tatcatttat ttgcttttca gtatcatttt tagggtcata
     8941 attataatac tttttagtaa aagtcaacat aattctaaca ctaataatac tacctattat
     9001 tgccgcaaca ttacctatta tagctggtag tacatattct ttaccttgag ctatcataaa
     9061 agttgttaca ataagtccca taccaaaggc tgttcctaaa ttacataaag caggtacttg
     9121 atacttctta aaatattgtg taaatgtttt gtctttagca aatggtataa tagctggatt
     9181 atcagataaa tatgttgcaa ctactcctgc tatactagca ccaggtaagc cccatatagg
     9241 tctcataaga cccttaaata tcttattaac taaagatatt actccaaact ctgaaagcaa
     9301 tgcactaagt gcacctgcaa gaactgccat agccattatt aaaaatactg tgtctaataa
     9361 aagcgcatgt gctgtactca ttattacttt aaacatcatt cctgcaccca tgatgcttcc
     9421 tacatatcca aatcctaccg ccaatagtac aataaatacg aaggtttcca tacttactgc
     9481 cttgactctt ctcttatccg acagcttttc catcttgaaa tcttccccct tttaatttta
     9541 aattaaggtt cctaacattg gtacaaattt tttggtacaa actgagttct aaaacttaca
     9601 ctcgtaatgc aagttgtcag ttttttgaaa taaaaaaaaa ttacgtttat agaataagaa
     9661 tatcctcttt aatttggata ctcttattct attttatgtt attatattat catccttttg
     9721 tttaaaagtc aattttttct atttttgttg tttaatttta tttatactct ttttatattt
     9781 ttcattttct tttatctttg taagtactcc atataagttg acttttctaa atacagtagt
     9841 tataaatacc aatcctaatg caattgaact tgcacacgat aatgtactca ccccatattc
     9901 tacagctttt atataattat tggtcaaaac agaatacata gtattatata ttccataacc
     9961 tggtactaat attattaaac agcaaacact taatgtagtt gctggagtca tataaattct
    10021 tgcacataac tcacaatata tcgcaaaaca aactgtagct ataaaaaaac ataagttttc
    10081 tgttactcct atatttaaaa gaaatttata tgaaaaccaa gctatacttc cacctattcc
    10141 tgctagtatt aagttttttc ctttcatatt aaatagcact ccaaagctta aggcagtaaa
    10201 aaaagctgct attacttcta taaacaaatg cacttatatc ccccctaact tgaagtacaa
    10261 tccaagtacc aagaacattc ctactgctat agatacacct acaaaaaata cttctgctaa
    10321 ttttgcaaga ccagaaatca actgtccatc taacaaatct cttaatgcat tagtcaaagc
    10381 aagcccagga actaagagca ttattgctcc tgctattaaa tggtctaacg tctttatgaa
    10441 tccgagcttt aaaattaaaa ttgaacaaga agctataata actgctccaa ttgcatttac
    10501 aaaaaagtca ttaagggaag atttacttaa atacataata acaaacttag tcagaatccc
    10561 aacagtaaat gcagctataa attcttccac atttcctcca aataatatcg aaaaagttgc
    10621 tgctgcaata cctgcaaaaa atattgttat taaatctgag taatagttat cttcacttat
    10681 ttcatctaaa attttctcac ataattcaat atctaaatta tttatactta ttgctcttga
    10741 taatgagtta acttcatgaa ccaagtttag gtctactctt cttgagctta ttcttttaat
    10801 tgctgaatgt aatttcccat ctacaaatac agatgccatt acagctgttg gagttgcaaa
    10861 tacatctacg tggtctattc caaaagattg acatattcta gctatcgttt cttcaactct
    10921 ataggtttct cctccacttt gcaacatagc tttacctgca tttgaggaaa agtttaaaat
    10981 tctatctata tccacaaaac atatctccca ttcttttata gtattattca tattttaata
    11041 caagatatta tttatttaaa ctattttaaa taattcatat ccaatattta cattttatga
    11101 aaaaacttgt tttttatgct tgtcaaaatc aaattttaag cttgtttaca ccataaaatg
    11161 gctgaattaa tatttgacaa acactttcaa ccatttatat aaccttctaa attgtataac
    11221 tttttactac aaataaaatt atatctcttt taattctttc tcttgagtat ttagcttata
    11281 gtaacttcat atttatatat gtacaatata aaacattatt tttatgatac aaaatataat
    11341 ttgtaaatgt atatatgtat taactgtgta tatataaatt attcttgacc ttatcagttt
    11401 tttcatatat cttcatctat accaaccata tattaataaa tgatattaca tagacttaag
    11461 tatattaaaa aacttaaatt tagttattta acaacataat actctaattt aatctataaa
    11521 aatccatact taatataatg caactcttat tttaacacca tctattacac aaaacaacat
    11581 attaaaaagt aaaatacaaa atatccacta taaaccttat tttaatactg aatatatatt
    11641 ataatatatt taatgtagct taaatatagt ttaaacataa agaaaggaaa cggtatatgc
    11701 taagaaaaga taaagttcta ttatctctac ataaattatg tgaaaatatg actttaaaag
    11761 accttcaatc agataaaaca ggattcacta caatagaaat tgctaaccat tcatctcttg
    11821 atagaagtaa taccagtaaa gaattaaata acctatacaa tgaaaaaaaa gttctgaaaa
    11881 tatctggtaa gccaatttta tttctacatg ctagtattat agaaaattta attggccgta
    11941 ctttgaacag tgatgaattt tctataaata attgtaatga gctacttaaa tcaagtaaaa
    12001 atgactcaga agatattttt tcaacattac ttggacatga ttctagtctt aaactaccta
    12061 tagaaaaagc taaatctgct atactctatc ctccaaaagg tcttaatacg ttactaatag
    12121 gacctactgg tgttggaaaa agtacatttg ctgaaacaat gtacaaatat gctgtagaaa
    12181 gtaaaatctt ttcttctgaa agcaaattcg tagtatttaa ctgtgctgaa tatgcagaaa
    12241 atgctaactt attattaagt aatttatttg gttatgtcaa aggttctttt actggtgcta
    12301 ataaggataa attcggcata gttgcacagg ctgatggagg catattattc ttagatgaaa
    12361 tacacagatt gccaccagaa ggtcaagaga tgctttttct tttgatggac aaaggcatat
    12421 acagacgatt aggagaatct gacattatgc acaaagctaa tgtctttatt gtgtgtgcca
    12481 caacagaaga tatacattct tctctgcttg ggactttttt aagaagaatc cctgttacaa
    12541 tcaacctacc tgctttacag gataggtctt taaaagaaag attatctctt attaaatact
    12601 tctttattat agaatcaaaa tacaccaatg caactataaa agttcacaaa gatgtaatta
    12661 aatcattact cctttatgat tgttttggta atataggtca gcttaaatct gacatacaac
    12721 ttatgagtgc aagagccttc ttaaattaca aaactggact taaggataat atagaaatag
    12781 atttatccct tgttcctgat tacatctata ttggtctttt aaaattgaat gaaaatcgta
    12841 gtaatctaaa taaattatca gaacttgatt atatgctttt ttatgaattt aatggtaata
    12901 acttagaagt tgaatataca aaagaactat ctaaatacaa tatagataca tcaaacaatg
    12961 gatttaaata ctctacagag attccttatc tggaaaatga tataaggagt atgtttataa
    13021 attacatgga aaagtactct acaaaattac tttcgatatc ttcagtaact cattctgatt
    13081 atacaagtaa tgagattttt aaagtaatca atccaaagat ttactatgct gttgaacatt
    13141 ctttaaaaga ggctgaaaaa atatcgagaa acaaatacac aaaacaaact tatattgctc
    13201 tttcaatgca tataagtgca cttatagaaa atataaatca agacacatat aatcctaagt
    13261 acaatttata taatgataaa attttcttag ataaagattt aaatatagct aaaatagttt
    13321 taagtataat tgaaaatgac ttacaaacat ctttacctaa agatgaagta aaatttatat
    13381 ccatgtttct aaatagcatg tatcttgaac caaacaatca atctgaaagt ataggagtca
    13441 taatacttgc tcatggaaat tcaacagcaa gcagtatgtg tgatgtagca aattcattgc
    13501 ttggtacaaa ccattgtaat gcaattgaca tgcctcttga tgtaaaagta agtaccatac
    13561 ttgataaaac tatagagttg gtaaaagaat gtgacgaagg taagggtgta cttcttttaa
    13621 cagatatggg ctctcttata gcttttggag aacttatttc agaaaaaaca aaaatcaaaa
    13681 ctcgctcaat tgaaatggta tctactccaa tagttttaga agcagtaaga aaaagtacac
    13741 taccagaaat gactttggat gaattagcta attccttaag ctctttaaat ccatatattg
    13801 gacgagcagc aatcgattca acagaaataa actatcctga aaagtcaaga gagtatgtaa
    13861 taataactac ttgtataaca ggatatggtt cagctatgaa agtagcagac tttataaatt
    13921 cttcacttaa aaatattagt aaatacaata ttaatttagt tcctcataat atagaaagtt
    13981 ttaaaatgta taagaataaa tttagtaata ctaaaattct ggcagttgtt ggaagtgtag
    14041 attttaatgt agccaacacc ccttttatac acatagagga actactaaat ggatatggcc
    14101 tagatatgct aaacaaaata atagaaggta cattcaatgc caatagtatt acaaatataa
    14161 aagaaacaaa tctatcatac aacagtttaa taataaactt tatgacccaa aacttagagc
    14221 ttttggactc tgttaaatta ttcaactatg tcaattcttc cttatttaca ataatagaat
    14281 taatagatga aaacttaaag agcaaattta agctaggata tatactacat tgctctttta
    14341 tgatagaaag atgttttaaa aatacaactt ttccatataa taacattact gaattaatta
    14401 gtaaaaatca tgaactatat agtttaatac gtgaagcctt taagccaaca gaagcttatt
    14461 ttaatatttt gataccagac gaagaaattg cttatataat agacatgatt aatgagtatc
    14521 taaataaata aaatttatag cacttatttt gtgcatatta attaatatat gaaatggagg
    14581 acgagatttt gaaaggaaaa ataaaacagt taacaatact tgcattaatt tttatattta
    14641 taactcctgt atttgcattt gcagatactc caccagtacc aaattcttca agagcagcac
    14701 tacttataga ccaggaaact aaaagaatat tatttgaaaa aaacattgat gaaaaaatgc
    14761 cattagcgag tttaagtaaa atgatgacct ttttattggc aatagaagca gttgataaaa
    14821 atcaagttaa agagactgat atggtgaaaa tagacaagtc tacagcttca gtaggaggtt
    14881 ctacttgtaa acttaaagat ggtgatgaaa tatctctagg agagcttatg cagggtctaa
    14941 tgctagtatc tggaaatgat gctgctatag ccatagctaa acatattggt aaaactgaaa
    15001 agaattttgt aaatatgatg aacaaaaaag cagaagaaat aggaatgatt gatacgtatt
    15061 attttaatcc aaatgggctt cctatatata ctgacccaga acataaagaa cctcctattg
    15121 aaaatatgtc aactgctcac gatatagtta cacttggaaa gtatatgtac gaccattatg
    15181 aaaatcaagt tacaagaata accactatgc aagtatataa tgatactaaa aaggatttta
    15241 cacattataa cacaaatcct ctgcttgttt cagtacctgg tgttgatgga ataaagacag
    15301 gatatacaga taatgctgga tattgccttg cattttctat gatggtacct aaagatgcta
    15361 aaaacgaaag aaatcataga ttgataggtg ttgtacttgg tgatggtaac aagaaaaata
    15421 gaatttcttc ttccgctact ttattgaaat atggtaaaga taatttccat tctaaaaaga
    15481 tagcacataa gggtgatatt atagagactc cttgtgttga tggtattgat gattttaaga
    15541 taacagttaa ggttgataaa gatttgtatg gtgttgtttc agataacgaa aatatcaatc
    15601 ctaaggtagt gtttaaaaat atgaattatc ctatccataa gggagatatt gttggtgttg
    15661 caaagtatta taatgactct ggtaaatttg ttggaagtgt tgatgtaaaa agtgaaagta
    15721 atataggatg tattcctttg aaggataaga ttaagattaa ggttgctaag ataaataaaa
    15781 aacttgagat taaaaattct gtttgtttta aagcatagta aagcatatta ttaaaagggt
    15841 atgttaaatt tataaagact ccactttgtg gagtcttttt gttttttaca aaaaattaaa
    15901 atagtggcaa caattgagga tgtgagggag agttgccact attttgtata gggtattata
    15961 ttatgttttt aagctagttt tactatcttc tatgtttata taataactct tgtgtgtgaa
    16021 tttaattgat aattaatatg aaaattatgt gaagttttga tttatttgta atatattcta
    16081 ctactattat aatttttcta ctggatatac taatcatttg tttaacatta tctgagattg
    16141 atttgcaaac ttttatataa ctaaattagt tatataactt atagattcca ttaaataccc
    16201 taataatcat atatgtacat acaaagtcat gcttatgcaa aatctgcagt ttcaactaac
    16261 ttattatata aaccatcttt atgtagaagt tcagcatcat ttccagattc tattactcta
    16321 cctttatcta atacatatat attatcagca ctcttaattg ttgaaagtct atgagctatt
    16381 ataataacta tcttatcttg tatgtactct ttcaagttgt tcaatatctc tttttgtgaa
    16441 atattatcta gagctgaagt tgcttcgtcc agaataagta cctttttatt acttactaaa
    16501 gctctagcta ttgatatacg ttgtttttga cctcctgata ttttgacacc tttttctcct
    16561 actacttcgt ccaatccctt atctagattt tttataaaat catctaaaca agtaacttct
    16621 aatatctcat ctaattcttt atcacttatt ttattattta atactatatt attttttatt
    16681 gtgtcatcaa atacaaattg atcttgagtt actatatcaa tattatttct tagtgatttt
    16741 aaattaatat cctttatatt aacaccatct attaatatat caccttcatt tatatcccaa
    16801 agtcggtata ttagatttgt tattgttgat ttgccacatc cacttccacc aacaattcct
    16861 gttacttccc ctttctcaaa ttgtaaattt atatcattta aaacaaggtt taaatcctca
    16921 ccatatctaa aagatacatt tttaaaatct attttcttaa tttcactgtt atctattcta
    16981 tatccattat tatcaacttt aatatctatc ttttcatcta ttactgaaaa tactctattg
    17041 atagacactt ttgattgtgc tattctagta ttcgccctta ctatagctat acatggtcca
    17101 attaacattc ctgtatattg ttgaaatgct attaactccc ccatcgtcat cttattattt
    17161 attattttat atcccccaat tccatatatt aatattgtta tcaatcctgt tagtatttga
    17221 gatattgcta tattactact cataatgatg ttcaaattca aatctttttt tatcaaatct
    17281 ctctctctct ttaaatactt attaaagaaa ttcttttttg aatttaatat aacaatatcc
    17341 ataatattag atacgtactc ttgaatcaaa tttgataaac tgcctgaatc acttcttatt
    17401 tcagaaattt tcttagatat aagttttgtc attttatttt gagaaaatgt taatatcaac
    17461 tgtaaaaata aaactactaa aaataaatca aactgcaaat taattaagaa aattaacgat
    17521 attatagctg taaacacatt tacaataata tcaaatataa tctctgctcc aaagttttct
    17581 atcataaata tatcactttc cataatggat aaaatattac ctgttttctt acttgaataa
    17641 tatctaccag aaagttttga tatatggtta agcaacttta cttttagatt tatagtaact
    17701 ttatttttta ttgaagtata taaatatgat aatataacat caaatgtagc tgataatatc
    17761 cttataaaaa tatatatcaa tattaaattt ataagcagaa aaatattttt atttcctatt
    17821 ccatcatcta ttatacatct aattagttga ggtattacaa tatttacgcc agcaattata
    17881 aaaaccatta taaatgatac tatcaactta gtcttattct tatatactat tatatttagt
    17941 attctaaata aattattatt aatagttttt ttcatctatt tttccatctt ttatagttat
    18001 aattctatca gctgtctttg caatattatc attatgagtt atcataacta gtgtttgttt
    18061 gtactcttta acacaccatt caagcatatt cataacttca tttgtagtct tactatctaa
    18121 gttgccagta ggttcatctg caaatattac tgacggtcta ttggctaaag ctctagctat
    18181 tgcaactctt tgttgttgac ctcctgatag ttcattcgga tatttcttta tttgtttttc
    18241 aatccccaac ttattaatta atctcataac atattcttta tcttcttttt tcccatcaag
    18301 caacacagga agtactatat tatcatatac atttataact ggtattaaat tgtaactttg
    18361 aaatatgaag ccaaattcct gtcttcttat ttcagacagc ttatcatcat ttaatgtata
    18421 taaatctaca tcatctaagt acacattccc taaagttggc ttgtctaagc ctgcaacaca
    18481 gtgaagtagt gtacttttac cagaaccact tgcacctaca atagctgtaa atttatgtgg
    18541 ctctatatct aaatcaatac catcaagagc tctaacttca ttctcatatt ttccatatat
    18601 tttttttaaa tcttttacct taagtatttt cataacgtat ctccattccc atttaaaaaa
    18661 catatatttt taaattaaat tttcgtaaat aagatctact gccatatcta aattctctaa
    18721 ttgatataag tatacttctt tgtagtcaat actaatatta aactctttat ttaaaatagt
    18781 taaaaaatta tatgcattat aatttccaat acatgtaaaa ttgttaaata aagaatattc
    18841 acttacaaat tcaaagtcta tttttatttt agaaaagttt ctatttatca catttattat
    18901 tttccttgct atatccaatc ttatcaaatt agatttataa cttggagtgt tcaagttttc
    18961 tatttttaaa tcttctttac ttttgctttt ttgaataagt atttcttcca attcattaaa
    19021 cttaatatat ggattgtttt ttaatatatt tgcaacaatg ccagttgcca ctgcacaagc
    19081 cttactattt ttaccaaaat gagttacttt atctttaaat ttaataaaat tatcttttga
    19141 atttgcatac atttgaatct tattttctgg ttcgtatata tattgattat ttttatatat
    19201 attttcatta cctactacac caataacact ttcaaatata gtaggaatac tttctttatc
    19261 ttcatattta ttatgttttg aacaaataat aaatctacct tgactattta aaatattaca
    19321 aaccttttct aattcttttt catacatact attaatagat gcagctgaaa tgtttattat
    19381 ctgtatatcc gacatagtca acttgctcag tgtactaaga agatttaaag aacttgtttt
    19441 tccagaatca ttaaatatat taataggata aatttgggta tttttactta tatttaatat
    19501 ggttttcgat acaaaagttc catgtccatt tatgtcattt atttccttcc cccccaaatc
    19561 tttcaattga aaatttgaat cataaacata atttccatta atatcatcat catttacatc
    19621 taatcctgtg tctaagactg ctattcttac tttagtcaat taataatcac ctctaaaata
    19681 aattaccttg aatataaaac taatccttac cataatatag gttccaagat gtttttcatt
    19741 tttttacaat ttgcatcaaa catttttttg ttatttttta aagtatatat tttcgatgga
    19801 caagttgtac aaaataaatt tactttacat tctttacaac tctcaatatt tactggtttt
    19861 aatttatcaa atatattaaa tatatccatt tctctattaa gaatcctctc taatatgttt
    19921 tcattaaatt ctaatatatt gaatatcttt aaatccttat actctaagtt tggacacgga
    19981 tatacatctc ctttatgatc tatacttatc tgattaacac ctgctttaca catatttgaa
    20041 cataaactac tttcatccac ataatcatct ttgcaaatgt aatcaatatc aagtttatca
    20101 ttaagatatt tgctatagtt ctcattacct ctaccaatat tcataaaatt tcttattata
    20161 ggcgttgtac ctaaatcttc atttaattta ttgaatttct ctatgtcttt ctcattattc
    20221 tttcctacca ccatagacaa acctacgttc tcgaattttt ttagttttat aagtctaatc
    20281 acatttaata ctttacagaa tactccttta cctcttactt cactacaact taattcatca
    20341 taaccatcta aactaatttc tattgcatat aaaccattaa ttatatcatc aatctgtttt
    20401 tctttaatta aagttgcatt tgtagacaga attattttac ctttaaattt ttcttttata
    20461 taatccaaca gctctaagaa atcatttctc atcaatggct ctccacctgt aatggttaat
    20521 atccttggat ttaatttaac tatattatct attacttttc ttacttgaga tgtatctaaa
    20581 taatcgttat tatctatttt agaatctaca cagcaataat cacaacttag attacatcta
    20641 ttagttggag aaaatgaaat tttgtttatt ctctctagtc taaattcact tagaatagaa
    20701 tcacttagaa gtcctatatg ctctaactta tctataactc gttcataata ttttttatct
    20761 tcattttctt ggaatacatc atacaaatta ccaagttgta atgcattatc tactgaatac
    20821 tttagaactt cataacattc tattggaatt ttaacccatc ttccatttac tttattacta
    20881 agaataactt gattattatt tattattttt tcacatatac aatctaaaaa atatttgttt
    20941 gagctattca tttattttat ttccttccta tatatttaaa ttagaaattt cattaatttt
    21001 cttttatacc acttactata ctaagtttat ttatcatttt attagaaaaa taaactgcaa
    21061 tgacacaaac aaaaacagat atagatccaa atattagaat ttcatatatt ggaatttcaa
    21121 atttaataga gttatttata ccatatacct gttgattaag tttgttagtt tcagaaacta
    21181 ttttataata attgaaacta gatataattc cagctacaat catacttaat accccatata
    21241 tcatattttc ttttattaac attcttttta catttttttt ggacatccct attgctctaa
    21301 atgtacatat ctcactcaac ctcacaatta tatttgatct aactataaaa ataatgttta
    21361 tagatgatat taataaaact ccaaatacac aaatcattgc taatttatat gaggatttat
    21421 tataatggtc tccaaaatga ttacgtacat atcttccctc tatatctgaa aatccacttt
    21481 tttctattat attatttatt tcttttatta cttttttatc tgtttctttt tttatcttca
    21541 caaaaacttt attataatca cttatccctg ttaattcttt taaatccttt tcattaaata
    21601 taatttgaat tccacctgat tgagattctc catcttgctc tgatatatag ctatttttca
    21661 ttattccagc gattcttact tttgtattta tatatttata ctctccatct ttatatacag
    21721 gaatttttat atctattaag tctcctattt taggtgattt tataatctga gtattatttg
    21781 acaatttaat tctagaataa aaattattta ccaataaaac atttttataa tccctgtctg
    21841 tataactata aacattttcg cctttatcta tatatttatc tatattattt aatagctctt
    21901 tattgtaacc tcgaattaaa actgtagact cattattatt atttatttca gttcttttta
    21961 gctcatctaa ataatcttct tctaagtcag catttcttag atatcctttt aaataaatat
    22021 tgggttcaac atctgttaca tcttcaatag cttttatttc atttaataaa gaattatcat
    22081 atttagaaaa tgtataattt gcatttattg gtgaatatcc caaataaata tcaaaattgc
    22141 catatgattt acttgttata ttagctgaat ctgactcttt tccatatatg aattttgtag
    22201 aagttagtct gcctataaat aaaaccccac atagtgatat cgtaattatt attattattg
    22261 atgtaacctt attcctaaat atatttttaa aagctatctc atttataatt ccaacaactt
    22321 ttttatgtga ttttaacaac aaaagcctag gaatataatt taacttatct gtctttttta
    22381 tagcatcaat tatagataaa tttccagctt tatatattgg tactacagag gctaatataa
    22441 ctgctataat tgacatgatg atagattcaa ttatagagtt tgtatttata gttaattctg
    22501 cacttttatc atatactatc atgagaccta agtaagaaaa aactatacct ccaagtaaac
    22561 caattactat acctacagat aaaactacaa tactctgaat aaaaaatacc tgtctaatat
    22621 ttctcttgct agctccaaca gctctaagta aaccaatttg cttggtcata tctagtatta
    22681 ttatgttaaa catattgtat attactaatg ttgcacataa taaagggagt aagctttgct
    22741 ttattgaata agttgttttt tttgaaatat ttttcatata catagacata cttacttctg
    22801 tatttggata aaattgatta cttgttaact tatattttct ccctagattc actacttttt
    22861 gagaatctaa atcttttgta ttcaatttta atgtacccat atgagttatt aattcatatg
    22921 gaacaatatc tttgccattg tttgaatatg taaaagctct taccttatat ttatttaaat
    22981 tttcatagta ttcatcagat ttacttaaaa taccaactat tttaaattgc ttatctttac
    23041 tataaataga attttcatta tttttattta tatgctcatt aacaatctga aaatcaatag
    23101 tttgatttaa tttgacatct aattccattt tgtctaaaac tttttgttct attactaatt
    23161 cattagaagt tctaggctct ctacctttaa ctaacttata tctagtagaa tctaaaaaat
    23221 ctttattgta agagcataaa tttagagatg ctccattatc tttacttata atttctccaa
    23281 atttaagtac atcattgaat tcttttatat cttcttcacc attaagttct tttactatat
    23341 cactatttat atcttcataa aaagcatgat aatctcccga gtttttgtat gcatgattta
    23401 tatctgattg tccttgagat tcattaataa cacttaatga aaaaactagc ataacagcaa
    23461 tccctatact aagtattaat gataaagttt ttcctttttg tctctttaaa tatgacaccg
    23521 caagtttaat atttatcata atcagaatcc tccttattca tatttacaag aaaaactcta
    23581 taacctaaaa tagtaggtta tagagcacta ttcattaaat actaactaac tcgttgtgct
    23641 agttaaattt aattattaaa aatactagag ttgagaaaat cacatttcat ttgtaaaaaa
    23701 ctacatctag tactttcaac atatttaata gactgattag tattttttaa taagctactg
    23761 ttccttttgg agttgatcct gcacaatcca ccatacagtc tatttgtgaa attattccat
    23821 gcttatcata ataccattta catggatctg cttgcacctc aacatcttga agttctattt
    23881 tttcttcgat tttttttata tgtatcataa tacatatccc cctttacttt tgttttatat
    23941 ttattataac tagttaaact agattataaa ctacatagtt tttttgttgt ttccacataa
    24001 actctttcta atccacaata aattggtact gcactttgga atttacctgt taccttttta
    24061 ttgataagtt tacatctggt tgacatacaa taatcaagtc tagtacatcc atcacactct
    24121 tcattcttag atatataaat ttttataagg ttcttaattt ttttatcatc taatccatca
    24181 tatatatttc ctagtaaaat atctttatct cctacacata ctgtacatgg atatacatct
    24241 cctctagtat ctattgcata actatttatc cctccaaaac aatctccttt tttcttattt
    24301 ataaattgtt tatctaaaat agatatatct atatcaggat aatccttttt aatattcata
    24361 attttactaa tttgttcatt taatatttta atatgagaat tatcccaatt tttatccata
    24421 aaatctgaaa ttggtgcgat atttttaaac ccttgttttg ctatatcata tacactctca
    24481 taaagatact taacagtatt tgagttatat gtcattcttg ccctaacata tataccttta
    24541 tctataagtc tttttccatt ttccattact ttttcataag tacctactcc gttagcatct
    24601 attctattta tatcatgtat attctttttt ccatcaatac ttatcgatat gttttttaca
    24661 ttctttgcta taaactctat tatttcatca tttataatag ttccatttgt agttatatca
    24721 aataatatct ttttgtcact caatctatct actaaatccc tatgtatata tttaatcaaa
    24781 tcaaaattta taaatggttc tcctccatgt aaaacaattg ttgtataatc actattactt
    24841 tctaattctt tattacaaat aaaatctatt gttttatcag cagtttcata actcatattt
    24901 tctttttttt tatcattacc ttcataacaa tatgtacatc tcaaattgca gtcttcagtt
    24961 acaaatatag taaatttcaa ttaacttttt catctcccca tacagctgta tttaaattgt
    25021 gtttcatcat actacaccat ctatccaatt ccaattcatt atccttaact gaatctaata
    25081 tcgctgggca atgccaacaa aaaatattta catcacaatc tttacatttt tcaaagttat
    25141 atggataaat actttctaat ttaatcaaag actttagatc tcttgtttta ttttctttta
    25201 taacttttac aaattcattg tcaaatatat ttcccatttt atagtcatcc tttatcagtg
    25261 aaggacaagg atatacatct ccatcataat ttataaatat ttgagagttt cctgcattac
    25321 atgcacaaca tcccattttt ttattttttt tatctttaca catttttaat tcattatcta
    25381 aaatatacgg aattgtaatt ggaagaactg tatcttcaat taaatagctt tctttatttt
    25441 ctaggcctct tcctgttgga ataaaatatc tttcaacaaa ttctgttcca agctttttat
    25501 ttaaatcttt aaattgttta tttaaatgag aatttttttg tccaaatacc atagacagct
    25561 ttatattact gaatccattg tcttttagca gattaacaga gcttattact ttattaaata
    25621 caccttttcc tcgtgtttca ctacaagtat tttcatctac tccatcaata ctaatatcta
    25681 ttttttcaaa attagttata ataaaatcaa cattatgttc atctattaaa gttccatttg
    25741 tggataatat taatttggta tcaggtaatt tttgttttat atatttaatt atttcaaaaa
    25801 aatcttctct tatcaatggt tctccaccag ttataattat atgaggagta tttaaaactg
    25861 atattatatc tacgatattt tttaaatcat atgtactaag taaatctttc ttattagaag
    25921 aagattcaac acaacaatgt ttacaaaata aattgcatct atttgtcaaa caaaaataaa
    25981 ttgcaacacc attgcttaac atatcaaagt ttaaattgtt acttttaaaa atttgatttt
    26041 tgttcataat atctataaga ttaataatat attttttgtc actttcatca tcaaaataat
    26101 ttacaaaatc ataaattttt attttatttt cttgtcctaa aagaaaa
//
LOCUS       DAMJZE010000033        26094 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJZE010000033 DAMJZE010000000
VERSION     DAMJZE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.033"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(76..219)
                     /gene="scfA"
                     /locus_tag="PC541_001297"
     CDS             complement(76..219)
                     /gene="scfA"
                     /locus_tag="PC541_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2838768.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(285..647)
                     /locus_tag="PC541_001298"
     CDS             complement(285..647)
                     /locus_tag="PC541_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2838769.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(705..998)
                     /gene="yajC"
                     /locus_tag="PC541_001299"
     CDS             complement(705..998)
                     /gene="yajC"
                     /locus_tag="PC541_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2838770.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1132..2253)
                     /gene="tgt"
                     /locus_tag="PC541_001300"
     CDS             complement(1132..2253)
                     /gene="tgt"
                     /locus_tag="PC541_001300"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2838771.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2332..2817)
                     /locus_tag="PC541_001301"
     CDS             complement(2332..2817)
                     /locus_tag="PC541_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838772.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2829..3854)
                     /gene="queA"
                     /locus_tag="PC541_001302"
     CDS             complement(2829..3854)
                     /gene="queA"
                     /locus_tag="PC541_001302"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2838773.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4172..5191)
                     /gene="ruvB"
                     /locus_tag="PC541_001303"
     CDS             complement(4172..5191)
                     /gene="ruvB"
                     /locus_tag="PC541_001303"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2838774.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5298..5900)
                     /gene="ruvA"
                     /locus_tag="PC541_001304"
     CDS             complement(5298..5900)
                     /gene="ruvA"
                     /locus_tag="PC541_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2838775.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5893..6402)
                     /gene="ruvC"
                     /locus_tag="PC541_001305"
     CDS             complement(5893..6402)
                     /gene="ruvC"
                     /locus_tag="PC541_001305"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2838776.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6714..7343)
                     /locus_tag="PC541_001306"
     CDS             complement(6714..7343)
                     /locus_tag="PC541_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838777.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7546..7869
                     /locus_tag="PC541_001307"
     CDS             7546..7869
                     /locus_tag="PC541_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2838778.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8137..8991
                     /locus_tag="PC541_001308"
     CDS             8137..8991
                     /locus_tag="PC541_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838779.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9177..10613)
                     /locus_tag="PC541_001309"
     CDS             complement(9177..10613)
                     /locus_tag="PC541_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2838780.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10684..12066)
                     /locus_tag="PC541_001310"
     CDS             complement(10684..12066)
                     /locus_tag="PC541_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2838781.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12202..13107)
                     /gene="garR"
                     /locus_tag="PC541_001311"
     CDS             complement(12202..13107)
                     /gene="garR"
                     /locus_tag="PC541_001311"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2838782.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            13503..14174
                     /locus_tag="PC541_001312"
     CDS             13503..14174
                     /locus_tag="PC541_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2838783.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14277..14825
                     /locus_tag="PC541_001313"
     CDS             14277..14825
                     /locus_tag="PC541_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838784.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            15087..15395
                     /locus_tag="PC541_001314"
     CDS             15087..15395
                     /locus_tag="PC541_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838785.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15557..17347)
                     /locus_tag="PC541_001315"
     CDS             complement(15557..17347)
                     /locus_tag="PC541_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838786.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17347..19074)
                     /locus_tag="PC541_001316"
     CDS             complement(17347..19074)
                     /locus_tag="PC541_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838787.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19319..19585)
                     /locus_tag="PC541_001317"
     CDS             complement(19319..19585)
                     /locus_tag="PC541_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2838788.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            19883..20995
                     /locus_tag="PC541_001318"
     CDS             19883..20995
                     /locus_tag="PC541_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2838789.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(21359..21868)
                     /locus_tag="PC541_001319"
     CDS             complement(21359..21868)
                     /locus_tag="PC541_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2838790.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(21870..22517)
                     /locus_tag="PC541_001320"
     CDS             complement(21870..22517)
                     /locus_tag="PC541_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838791.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(22531..23703)
                     /locus_tag="PC541_001321"
     CDS             complement(22531..23703)
                     /locus_tag="PC541_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2838792.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            23971..25347
                     /locus_tag="PC541_001322"
     CDS             23971..25347
                     /locus_tag="PC541_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838793.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(25597..25956)
                     /locus_tag="PC541_001323"
                     /pseudo
     CDS             complement(25597..25956)
                     /locus_tag="PC541_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT         8811 a         4444 c         3058 g         9781 t
ORIGIN      
        1 taaaagagga tacctcataa tgataaaaga atcactttga gataccctct tacaaacaac
       61 taaatcaata attttctatc tttcacactc ttgatttgca actgtacaag aagttttaca
      121 agctgattga caagaagttt gacactctcc acatccacct tttgcagcac tttgctttaa
      181 agtagcttga gataaagttt ttacatgttt attttccatt ttcaacgcct ccatttctaa
      241 ttgtttccta ttgattatat cataaaacta ctttaatata aactctattt aaagttgaca
      301 cccaatacgc ctccaatgcc tcctgctgct gatattaaca ataacttata aatcatccct
      361 acttcaaata caaacttttc ttgtgctaaa aacacagtca aaaaaataca tactatgtac
      421 aaaagtccta ctaaaagccc atacataagt cctttttctt tcatgtgttt tgaagtgtaa
      481 aaccctccta tagctgcaga aatagtagtt ataaatgatg agaccatagt tatattatct
      541 ccaccaatat ctgtaaatgt caaaaataag ttatacacta ataaaacagc caaagttatt
      601 atatatgcat atcctagacc tttaaaaata taattagatt ttcccataaa aaaacctcct
      661 ctcaaattaa tatatattcg agaaaaggct tttaaattac taatttagtc ttcttctatt
      721 tcttcttttt tagatttaac agttcctata gcccattttt caaatggaac tttagttcta
      781 tttggtccta attctaatat tacaacatca tcttcaactt tagctacatt agctactatt
      841 ccacctattg taatgacctt gtctcctaca cttaggctac ttctcatttc ttttaattgt
      901 ttgtcttttt tcttttgagg tctaatcatt aggaaataaa agattgcgat tactactacc
      961 catagaccta tactcataat tatttgttgc tgtggcatta atttttcact cctttgtaaa
     1021 ttatactctt ttgtaaatca taataaatgt ttatataaca atttactttc tctatctttg
     1081 agtactattg aatattatat attaaaatga ttataattaa tagtgcaaaa cctatatttc
     1141 atatccatat tttgcaaaaa attcatttct aaaatcaagt aatctatctt ccattatcgc
     1201 ttgtcttatt tgtttcatta aatttaataa aaagtgaaga ttatgagtag ttattaatct
     1261 tgcacccagt atttcatttg cttttataag gtgtcttata taagctcttg aatagttttt
     1321 acaagcataa caatcacatt cagggtcaag tggagtaaag tcttcagcat aagttgcatt
     1381 tctaactact actttacctt ggctagtcat tgcagttcca tttctagcga tacgagtagg
     1441 taggacacaa tcaaacatgt ctactcctct tataacacct tctactaagt catctggact
     1501 accaactccc attaggtatc ttggtttatc ctttggcata agtggagttg tatgctctaa
     1561 cacgtcatac ataagtggtt ttggttctcc aacactaagt ccaccaatag catatcctgg
     1621 taaatcaata ttagttattt ctttcgctga ttgttccctt aagtctttat acatcccacc
     1681 ttgtattata ccaaataaag cttgcttgtc ggtattatta tgggcatctt tacatctttt
     1741 taaccatctt gttgttcttt ctaaagaatt ttttacatat tctctatctg atgggtatgg
     1801 tgcacactca tcaaatgcca tcattatgtc tgaacccaat gcattttgta tttccatagc
     1861 tttttctgga gttagaaaat gttttgaacc atctaagtgt gacctaaatt ctacaccttc
     1921 ttcttttatc tttcttaaag gccctagact aaatacctgg aaccctccac tatctgtaag
     1981 tattggttta tcccaattca taaacttatg aagaccacca gctcttttta taagttcatg
     2041 acctggtctc atatataaat ggtatgtgtt actaagtatt atttgtgaac caatctcttt
     2101 taattcttct ggtgtcatag attttacagt tgcttgagtt ccaactggca tgaatattgg
     2161 tgtttcaata atcccatgtg gagtgtgtaa tcttcctaac cttgcaccac tctgcttaca
     2221 agtctttatc aattcgtatc ttactgcgta cataaagttc tccttaatca tatttatttc
     2281 tcattatttc ttaagatata tttgtaataa gctattcata taatttattg tctaaataga
     2341 tttatcttcg tcttctttgt tctgttgttc cttaattgct tcttcatcat gaatactcat
     2401 tatattaaga gcactcttgt cttttgttac taaaaacttt tttatagcaa atagtcctgc
     2461 tatagctata actcctatta aaacatctat cataccttca tttttaggca gtagcaacat
     2521 ttttctagca attgcgtaaa gtaatgcctc aataaatgct cctggtatat gaagagatag
     2581 cattactaca agttcaacac ctattactaa aagtaatatt tgtgctaaaa aatcattaag
     2641 ctgactgtat tgtactggat tttgaaaatc aactatatat gctccccaca taagtcttag
     2701 aacatctata gttcctaaaa atactgctat tagaactact actgctaaaa cacgttcaaa
     2761 tataaaagca atctttaata tagggttatt ctttttttgt ttttgtaaac tatgcatcta
     2821 ttacctccct actttataat catagcatct ccaaaactga agaatctata tctttctttt
     2881 actgcctcat tatatgcatt taaaacatta tcctttccac aaatagcact tactaacatt
     2941 attagtgtag actctggtaa gtgaaaatta gtaataagtt tatctaaaac tttaaattta
     3001 taacctggat atataaatat ttctgtccat ccactagttt ctttcatctt tccatcctca
     3061 ttacaagctg attctatagt tctacaacta gtagtaccaa cacatattac attttttcca
     3121 ttttcctttg ctctatttat tttgtcagct gcttcttggc taaccatata atattctgaa
     3181 tgcatcttgt gatttaaaac atcctctact tttactggtc taaaagttcc taagccaaca
     3241 tgtagagtta caaaggctat atctacacct ttttctttta ttttatttaa caattcttct
     3301 gtaaaatgta atcctgctgt aggtgctgct gctgaaccat tatgctttga ataaacagtc
     3361 tggtatcttt ctttttcatc tagtctagca gtgatatatg gaggtagtgg catatttcca
     3421 agttcatcca atatttcttc aaaaattcca tcataatgga attttataat tcttgaacct
     3481 tcatctgata aatctacaac ttctccaatt agctttccat ttccaaaaga aaatttagtc
     3541 cctacttttg ctctttttcc tggttttact aaagcctgcc aagtatcttc ctctgttctt
     3601 tttaataata aaaactctat tttacctcca gtttcaagct tctctcctat taatctagct
     3661 ggtataactc tagtattatt taatactaag caatctcctg gatttaaata ttctattata
     3721 tctctaaata ctttatgttc aatattacct gtctctttat ctaataccat caatcttgag
     3781 ctagctctat cttctatagg aacttgagct ataagttcct gtggtaagtc aaatttaaaa
     3841 tcacttgttt tcaatgttaa acttcctctc ttaaaatttc tccagctaca ataatatcaa
     3901 aaaaacaact tattttccac aatattacca taatctatca agaaattgct atttttaaca
     3961 gacaccatat ccacaatata tagatatttt tccacgatat acatgcctta tccacaatat
     4021 atagatacaa tacacacaat ttttatcgtg tttatccaca gtatctacag atataattac
     4081 cattttgtgg atatgtttga atttttagcc attttagaaa tattttaatt taatgactct
     4141 tttattttat aaatactcgt ataatctatt tttatttttc attgggatat ggaattttta
     4201 agtgttcata tgctaatggc atagctactc ttcctctagg acctctattt ataaatccta
     4261 attgaagcaa atatggctca tatacatctt ctattgtatt tctatcttct ccgatagaag
     4321 ctgccaatgt atctaaaccc actggtcctc ctctaaattt ttctattata gtcataagaa
     4381 gcttctcatc tacaaaatcg agacccaaac tatcgactcc taaaagttcc agagcatctt
     4441 ttgccacttt atcagttatt tttccatcag ctctgacttg tgcaaaatct ctaactcttt
     4501 ttagtaatct atttgctatc cttggagtcc ctcttgaacg ttttgcaagt tctaatgctc
     4561 cattagactg tatctctgca tctaagatgc ttgaagacct aagtactatt tttgaaagtt
     4621 catcaactgt ataatagtcc aatttacaaa taacaccaaa tctatctcta agtggatttg
     4681 taagcatacc agctcttgta gtagctccaa taagtgtaaa tttaggaagg tcaagtctta
     4741 tgcttctagc agatggccct ttccctatta ttatatctag gcaaaaatcc tccattgctg
     4801 gatataaaac ttcttcaaca cttctattga ttctatgaat ctcatctata aataaaacat
     4861 cattttcatt taaattcgta agtatggctg ccaaatctcc agctctttct attgcaggtc
     4921 ctgacgtaat tcttaaattt acccccatct cattggctat tatacttgct agtgtagttt
     4981 ttccaagacc tggtggtcca tataaaagca catggtctaa ctgctcattt ctcgatttag
     5041 ctgcttctat aaatatacta agttgttctt tggatttttc ttgacccaaa taatcttcta
     5101 aagtttttgg tcttaaactg ttttctatat ctatatcttc catcttcatt gtagaagtta
     5161 ttatcctatt ttcatcttca aaaccttgca tttatacccc tcctctcgta cttttgaaaa
     5221 tctactttat atattttcaa atttacataa actccttaaa tattgtttta aaatccaaat
     5281 attataaaat ccaaatacta tttcaaggat ttactcatta tatatgtaag tgctttcttt
     5341 ataatagctt ctgtattcat accatctttt ttacattttt gaactgcctc ttttgattcc
     5401 gacaaagtat agcctaaagc tacaagtgca tctacagact catcttgtga tatcaaggta
     5461 ggtttttgtg ataataacgt tggttcaaac tctatgttat ttttgtctac cttatcctta
     5521 agctctaaga cgattctttc tgctgtcttt ttaccaacac caggagcttt ggataatttt
     5581 ccaatgtctt cacttaaaat atatgctcct aactgtgctg gtgatgcaaa tgatagtatt
     5641 gaaagcgcca ccttaggtcc tattttagat acagatgtta gtaattcaaa catcttaagt
     5701 tcttccctac ttacaaaacc acaaagactc atatcatctt ctcttacaat taatttagta
     5761 tatatttttg tggcttctcc cacttgtaaa ttcattattg tattggatga tacatttatt
     5821 ttatatccta tcccattatt ttcaacaact atactgtcta tataaatttc ttctactgta
     5881 ccttttatat aactatacat acttaccacc tatattcttt agagtttttt caagtttgtt
     5941 tgaatgagca tggcaaatag ccacagctaa agcatctgca acatcatctg gttttggaac
     6001 tttttttaga cttaaaaatg aagttaccat ctgttgaact tgtgccttat ctgctctccc
     6061 atatccaact acaccttgtt ttacttgaag tggagtgtat tcatatacag gcttcccatt
     6121 atgagcacat gcaagcatag taacacctct agcttgtgca actgttatag ctgtttttac
     6181 attcttgtta aagaataatt cttccattcc aacttcatct atattgtaat tctttactat
     6241 tgtatcaatt cctttataca caagttccaa tcttctcgat atattcatat gggcaggtgt
     6301 tgtaactgct ccataatcga ttgctttaaa cttgctattt ttgtattcaa ttataccata
     6361 tccaactatg gctatacctg ggtcaatccc caatattatc attttttaaa attcccccta
     6421 aaaataaaag aaataccaaa atgtatttct cataaaaact tatgttctaa atcattatat
     6481 cataaataat attaacaaat cataaaatgc tctatgtaaa atataaaatc aaataaaaaa
     6541 gttatatcaa agcaagattt tacctcaata taacttttat atttattaca aataaattat
     6601 tgtattttta taggaattaa atgtgactac aattttaata ctaatctatt taatattaaa
     6661 ttatttatta ttaatttact tagtatttaa cttaaaataa tattaattta ctattaactt
     6721 gcaaagtctt ctaatcttga ataggcctct tcttcagtat ccataactac accttttttg
     6781 atttcatctt gaactgtttt aggaagtgaa tctactctta tttttgtttt ttctactaat
     6841 gttttacttc cgctatcatc atatttatat agtgctatat ttccattatc attttcaata
     6901 gtatatttac cttttatagt aacatccatt gcttgtacat ctttattatc actatattta
     6961 gattctacca atatgatttc atttttatcc atactcttta tttttttagt tggatattct
     7021 tttttaaaat atgatactat ttctgacttc gtcttatcta ataatgactc tggaactatt
     7081 cctgccattg taagtgttct cttatagtta gtttcatttt cgccttcact ttgaagccaa
     7141 acttcacaat ttttatttaa tccaaaagct tcttttgact tttcattcat attcgcacta
     7201 gtacttacaa cttcaccagt ttgaactcca ttgtgtttta atcttgtccc tatataaact
     7261 ccgctactta atacaaatac tccaaccagt attaaggcag atattttcca aggaaataat
     7321 tcttttttct tcttttcaaa cattgattac gcctcctttt tctattattt tctccaagct
     7381 gtaagttttt atacaattta ggtaaatttt agaagaaaat atatttatat ttcaaattac
     7441 tctaagaata gatattttct aaaaggtagt tttctcttaa tggttatatt cttgtaatta
     7501 tatggacata atttataata aataatcgtt taggaggtaa taactttgga aagaataaac
     7561 tgtaatgtaa ttaactgttc acataacaac agccatatat gttatgcaga taaaattttt
     7621 ataaatggtg aaaaagctca caatgacaga catacttgtt gtagttcatt tttagatgaa
     7681 tctatttata gtaatttatc tagcaacgtt aataataaaa attgtgagga attagtttgt
     7741 actgtaaaaa attgtaaata caacaaagat ggtagcttat gtacacttga taatatattg
     7801 gttgctcctg aaactgatag agtaaacctt tatattgaaa catgttgctc tagtttcgaa
     7861 tgtaaatagt catagtataa atataagggt gtcaaatgta attaaactaa catagtgtag
     7921 agtatctaca actattagtt tgattatact tgacaccctt atttatttta aataaaattt
     7981 cattattata accatgttta taatgttcta actgtaatta aatttgataa atttctataa
     8041 attggtataa tcaatatgta aattgaattt agtaaattaa tcataaaaat aaaatatcga
     8101 gtatatagtg ttatactaac tagaaatgag gtgcttatga gtacatttta tattcaattt
     8161 ctttattttg taatttatag ctttatagga tggtgttgtg aaactacata ttgttcagtt
     8221 ttacaaaaac aatttgtaaa tagaggtttt ttaaatggtc cattttgccc tatatatgga
     8281 tttggagcat tagcaatagt tgctaccttg actccttttg tacacaatat tcctttgtta
     8341 tttttgttta gtataattat aactagcata atggagtact gtacgagctt tattttagaa
     8401 aaaatattta atatgacttg gtgggattat tctaaacata aatttaatat tcatggaaga
     8461 gtttgtctgg aaaactcact tatgtttggt gtattatcat tgattgtaat gcttatagta
     8521 catcctattg tggttgactt tattaactct atctctaaaa atgtgctatt tatatttgct
     8581 atatctatag aaatttattt tgttttagat ttagttatca cagtccatac aattttacaa
     8641 ttaaatggta agttaaaaca aatcaatctt ataattaagg aattaaaaga taagaaagaa
     8701 tactacaaac ttattacaca agaaactata gaaaacaaac ttgaatcttt agttgaaaat
     8761 agactggata ctttggaaga taggtttgaa atacttaaag aaaaagaaaa ttatcgctat
     8821 gctaaaaatc gtattgatga gctaaaaaat aaacttaata ctttgctgtc taaccacaaa
     8881 ttacttcata gacgtataat caaagcattc cccaacataa gctctaataa gcatacagat
     8941 attttaaata aaatcaaaga aaatattaaa tcaattaaaa aagataaata ataaaataaa
     9001 cacataagtt aaataataaa aatactaata cataagatta aggtttagaa tattttttgt
     9061 aaaatactct aaaccctaat taatatacta ttatttttaa tcttaattta taattttagt
     9121 ttattttaat tttaactcag taaatttaag cgtttaaact tcaatacact aatatactac
     9181 cccatcaata tctcaactga ttctctaagt gtgctaatct ctcccacatt tccagggaaa
     9241 attatatatg gcatattagg aaatttgctt tctttgcctg tcatccatac tggtatacca
     9301 ggttttatct gtcccattac tgtagctttt tttactctta gtgctttagt accaacatca
     9361 ctagaagtaa ttccaccctt agctataata aaatttggtc ttacattaag cataccaatt
     9421 atactggtaa ctgcatctga tatttctaca gaaatcataa gctgtttatc tttatcattc
     9481 gtatccaagt caaaacgctc tcgtctagta taaacagcaa cagttttacc actgcttatt
     9541 ttttcctcta caacttttat aactcttttt acctcatttt ttagaccatt ttcttgaaga
     9601 actaaatgtt gattaaattc tataaattct atcggatatt tacaattttt aagttcttct
     9661 aactgttggg tagtcttatt cacatgtgaa ccaacaagta taattcctcc attggtatta
     9721 ccttttgata ctaattcatc cttagtaagt agttccttgt cactcactcc gccaagtact
     9781 tttgttatgg cagcagcgct tctaaacata aattccttac caccatttac tgctcgtata
     9841 aacgcaattg taaatacctt tacatctaca taatcggttg catttacaat aattttattg
     9901 aatccttcag cttttttaag tttttctgtt atagcttcaa tatttagctc tctaagttct
     9961 tttgacgaaa tatatatcat atcactagat ttgtactttc catttgtctt ttcttcacac
    10021 cactcaccta tatcagatga tttatatcca aaagatttat cttttgcaaa ttcagtcatt
    10081 cctgctggta caagtatttc tccttcttta acataatgta cattatctat agtaaaccta
    10141 ccaccttctt taaaaaatgg cattattatt tcaccctgaa ataacttccc cgataatctc
    10201 tctacttcgc tttttaataa attagtttca agaggataat gtcctctcat agtagaatca
    10261 ctacgactta tgattataaa atccttatta actttctttg atacatccac aatattcttt
    10321 gaaatttctt tatgtgcttt tgtagtttga gcaacagtaa atccccttga attggtaagt
    10381 ataaaaaaca tactattttt ctcattaaag ccttgttcca tactatcctt atcccaatca
    10441 gtataaacag aaatatcatg aactgtttgt acacctgttg ggtcatcatc taatacaaca
    10501 atttttctat ctaaatgctt aagctcatct gcaagaagtt tatttaatct atcctcatct
    10561 acttgtttaa aagtattaag tatatctatg ctaattggtt ttacttctct catttaaaac
    10621 gcccctttat gtaatttgta tagtctaaat ataatttata tgatataaac aatagtttgt
    10681 tatttataga cctggaagtg gtataattcc atttataaca ctaagcaata aaaccattat
    10741 aaatgctaca actccagcaa tggttcctcc aattgtccaa gagtttaaag tatctttaac
    10801 tgataatcca ctaaatcttg aaagtaccca gaatccagaa tcatttggaa gggagaaacc
    10861 aactcctcca gcacatatag caagccctac taaaactggt gacattccag ttgccaaaat
    10921 tgtaggtcct aaaattgatg atgttgttac taaggctaca gtagcagaac cttgagaaat
    10981 tctaagcaag gcacttaata agaatccaag tactacaact gatatactaa gaccagacat
    11041 agtatcaact aagaagttac ctataccact tgcatttata acactgccaa acgcaccacc
    11101 tgaccctgtt atcaataata ttagccctgc tgaatcagca gcttcaataa ctacatctcc
    11161 cataggttta tttatgtatt tctttaaaaa tcctattgcc accaatattc caagaagtat
    11221 tgctatgttt ttatctccta taaatgcaag tactccactc attgtagaac ctttttctaa
    11281 gaacatacta aatacatttg atataagtat aagaagtatt gggaacaata aagaaaacat
    11341 agaaattcca aaacctggta ctttagaaga atcatcatct tcaattttta tgctatcttt
    11401 tccctgctct aaattttttt ctctatcatt ctcatcaata gcataagctg gatatttttc
    11461 aaaacgtttt ccataaatcc atccacctat aagagctgct ggaaaagcaa caattataga
    11521 ataaagtata aaactaccta catttgcatt tacatttgct gcaactgcca aaggtcctgg
    11581 tgtaggtatt acaagtgcat gacctactat agtcccaact cctaaggcac atacaaaaac
    11641 cattagagat ttcttagtta ctctcgaaac atacttaatt ataggcatca ttataacaaa
    11701 agctgcatcc ataaaaactg gaattgaaat taaaaaacct gctgtagtta ctgctaaagc
    11761 actgtttttt tctccagtct tagctaacaa cccctttgca atagattctg ttgctcttga
    11821 ttctgaaaga atatttccaa atattattcc aagtccaatt acaataccta tactacctaa
    11881 tgtatttcca aatccagtcg atattgtggt tgatatttca tttataggca ttctaaccat
    11941 aaagcctgta attatagatg ttatcagtaa tgccaaaaac ggatttactt taaatttaat
    12001 aatcgataca agtaaaattc ctatagatat aacaaatgct atcaaaagtc catatcctgt
    12061 aatcataatc tttacccctt tatcattcta ctttattatt tatcaatttt atttttcttt
    12121 tactatgata acttaacctc gctgtattta tataatctaa tcctaataat atgtatttaa
    12181 ttatattgat atatttaaat attaattatc gtgttttttt atttcaacac ctgccaactt
    12241 ttcaaaatac tgtgctattc cactatggtc atcttccaaa tgtccatgta cttttaatgt
    12301 ctgcataatt tctaataatt gacttgtcat aggtagtggt acatctaaat tgtgtgctgt
    12361 ttgcattaca ttcttaatat ctttcaaatt aatagatatt tttccacctg gtttaaaatc
    12421 acgattcata atcattggta tcttcacatc caatattgta ctaccagcta ggcctcctct
    12481 tattgcctta tatacttttt ctgggtctgc acctgctttt gctgcaagta caaatgcctc
    12541 agaaactgct gctatcgtca aattaactat tacttggttt gtaagttttg taacacttcc
    12601 actaccatta tctccaatta aaatagatga gcttcccatt atattaaaaa atggctctac
    12661 tttcttataa atttcttcct taccaccagc cataaaagca agtgtaccat caatcgcttt
    12721 tggctcccct ccacttactg gtgagtctat aaattctaag cccatatctt ttaatttatt
    12781 tgcacacaat attgattctg taggagtaac tgaactcata tcgactacta tacttccttc
    12841 ttttaaagtt tttgccaatc catccatccc aaacaatata tcctgcacaa tagttccatt
    12901 tggaaggatt gtaaatacaa tatcacattc ttgacccatt tctaaaacac ttgcgtattt
    12961 tgctccacat gatataagtt catccacagc agatttatta atgtcataca ccaaaagatt
    13021 acatccatct tttaataaat tttttgccat tggctttccc attatcccta atccaataaa
    13081 tcccaatttc attttataac cctccatatc ttcacatttt tgaaaacgtt ttttatgttt
    13141 tagagtatac tacataccag tataccggtc aatataaaat ataaaagtat ttattttaac
    13201 aataaatagt aatttttaaa gtcatttatt atatttagtc atcctatata agttttaata
    13261 catactattg ttttcaaata taatatacta cttttacagt tttgtatttt aactaatttc
    13321 aattagtttt aattaatctg cctttaatgt taatcatgct tcacacttca ataatttttt
    13381 atatttttta atcttatctg caatttaaat tgcaaaagat tgaccttatt ttcactatgt
    13441 tgtatgctta aaattatata agtttttgta tttttatatt gtttaggggg gtattattaa
    13501 gtatggctac atttaaaaga ttacaagaac aagcatatga ctacttaaaa gaattgatac
    13561 tatctggaga aatgatagaa aatgaaatat attctgaaac aaaacttgct agtgaaatag
    13621 gtatctctag aacacctata agagatgctc ttcaaagact tagtcaagat ggatttatag
    13681 atataatccc aagcaaagga tttagaatcc atcaaattac agcaaatgaa attgtagaga
    13741 tattccaaat acgctctgca atagaaggat tttgtacttt tttaattaca agtcaataca
    13801 aagaagctag agcagtagaa actataagta aattaaaaca tcttttagat aaacaaaaag
    13861 atattttatt aggtgataaa aatttaaact catttgcaga atacgataca ttatttcaca
    13921 caactatagt ttcatatgct caaaatactg aatttgacaa aatgtttaac aattatatgt
    13981 acagaataaa aaaattagct ctcgattcat tgtcacatga aggaaggctt gaaacaacat
    14041 taaaagagca cacagatatt tttaataata ttgcaaatgg atgtattgaa gatatttaca
    14101 gaactactct cattcatatg gaaactccta aatacattaa tttagaagat ttttgtaaca
    14161 ataagttatt ttagtattat actttcattt attaaaaata tatctatctc aaattaattt
    14221 cctaatttat aaaaaatatt ctataattat attgtaacta atacttattc taaggagtga
    14281 tttttttgaa cgaagatgtt gtaactaatg aaactattga caaagattat gctattgaag
    14341 aagttcgtat ggcatgcaaa cattttggtg atttatattt ttatttttcc aaagtgttat
    14401 ttgaagagtt tggtgaagat aaaactagcg aaatattaag aaaagtttta tttgaaaggt
    14461 ctgaagaaag agctattgct atgagagaga gagctcatga aaatggagat gaattaatcg
    14521 cagacaatat tattagcaca actgatgttc catttctagg atgggttcct gaatttgaag
    14581 agttacattg cccctatgga gcttcttggc ttttaagatt tgaggaaaat ccttggttta
    14641 aaaaatttgc ttctctttac tgtgatgtta cagatactac tgtagctgaa gtatttacag
    14701 gagatacgtc ccataaaata acaaaaaaca ttctatgggg agataaaagt tgtgaaagaa
    14761 tatatttcca tgatgacaag gtatctgaag gtaaatatac ttatggaaat aaaaactgta
    14821 aataaatgat ttaaattttc tatattcata tactatattt tgtacttttt catataaata
    14881 ttataactag tcttttattt tacttttgtt taacctaaag ataatttact tttaatctat
    14941 atgaattatc atataaataa tcagaaagtt atctttataa attatagttt tagatttata
    15001 atttataaaa tatttatgtt ctttgtaaaa ttttttagaa gcttctaatg ttatgctata
    15061 aaactatctt gagaagggct gattgaatgt ttaataaaat atatgaaaag aaagaattta
    15121 tagtttttca agttaaaaaa ggttatgtag tttataatac tagaaagagt tttgaagaag
    15181 gtcatacaca tttaaagcat tttgaagcag caaagacagc tattgattta gcaataaata
    15241 aaaagattcc tagaagcaaa gatggatatt atctgactag tttaattcgc ataagtgatg
    15301 atggttatta cattgataag ttaagcgaat tgttatatgt tagagaacaa aaaggtaaaa
    15361 aagaaaagta ctgtaattct ggttatcaga tgtaacaaga ctagttgtga attataagta
    15421 ccaatcataa gtacctttag tcttaaaatc aaagagtatc agtaaatcta taaaaaaaca
    15481 acttactgat actcttttta attatatgat taatatttga atttgataca aaattttata
    15541 caaaagttta tgttatctat tctgattctt tgtctgcaaa ttggctatta taaagcttct
    15601 cataaaatcc acccttttcc attaattcat catgattccc ctgctctata atacttccat
    15661 tattcataac taaaatcaag tcagcattgc gaattgtaga aagtctatgt gctataacaa
    15721 aactagttct accattcata agatttctca ttgctttttg aagcataagt tccagcctag
    15781 tatcaacaga actcgtagct tcatcaagta tcaatattga agggtctgat ataaaagctc
    15841 tagctatggt taaaagctgt ttttccccta gagatatatt agatgcttct tcatttaaaa
    15901 acatattgta tccatctgga agagtggtta taaagtgatg aacatttgct acttttgctg
    15961 cttgtataat ttcttcttta gtagccccaa atctaccata ttctatattt tcaaaaatgg
    16021 ttccattaaa taaccatgta tcttgaagca ccataccaaa cattgaacgc aaatctttac
    16081 gtttcatatc tcttatatct actccatcaa ttttaatagc acctttcttt acatcataaa
    16141 acctcatcaa taagtttatt aatgttgttt tacctgctcc agtaggtcca acaatagcta
    16201 ccatctgacc actttttact tctgcattta aatcttctat taaagttgaa ttttctccat
    16261 atccaaaatt aacatgctca aatgtaacat ttccttttac attttctatc ttaactggat
    16321 tttcaacatc aggtatttcc tcttcttcgt ctaaaatttc aaatacacgt tctatagctg
    16381 caaaagaaga ttgtattact gtagataact gagtcatttg tgaaagtggc tgatttatct
    16441 gccatatata acgcacaaat gcttgtaaat ttcctactgt tattcctcca cttactgcat
    16501 atatagaacc gacaactgcc acagctgcaa ttccaaagta agttactaat gaaaccatag
    16561 gattcatcat acttgatata aattgtgcct taaatccatt ttcacaaagt tcttgattta
    16621 cttttttaaa ttcttttata gaatcttctt ctttaccata tagttttatt tcattaaatc
    16681 cagtatacat ttcttgtact tttccattta attttccaag agcattttgt tgcaatgaaa
    16741 atctactttg agattttttt actacaaatt tagatacaaa ataactaatt ggaattataa
    16801 aaagagctat aagagccata tatatattaa ttgtaaacat cattgaaagt gccaaaatta
    16861 tgactaagat tgagtttaca atttgcataa aactctgctg caatgcattt gatattgtat
    16921 ctacatcatt tgatatacga cttaatacat ctccaaaact attactatca aaataactaa
    16981 taggcaatct tcttatttta ttttcgactt catttcttaa atctctcatt gtgttttgaa
    17041 tagcattcgt aagtagaaaa cttgaagtat acgtaaaaat tgcattggca aaatatatac
    17101 atgctaaaat caccaatatt tttgttacat aatcaaaatt tacggaagcc cctggtactc
    17161 cttttgcgat actaattaca tctttagtca attgtgttat tatcaatcct tcagttttag
    17221 gtgcaagtgc attcatagta gctgctgaaa ttatacatat aatagctata ataaaggaaa
    17281 atttatattt ctttacaaat ggaagaagtc taccaattgt tttcttaaaa cctttagact
    17341 ttttcattat tctaattcct ccttttttaa ctgagatgtt gcaatttcat agtatatttc
    17401 acagtttttt aataattctt tatgagttcc cattcctaca acttgtcctt cattgagtac
    17461 tataatctta tcagcatcaa taatagaact aattctctgg gcaacaatca atacaattgc
    17521 atcttttgtt tccttcttca atttagcacg aagtattgca tcagttttaa agtcaagtgc
    17581 agaaaaacta tcatcaaaaa tgtatatttc aggtcttctt ataatagccc ttgcaataga
    17641 aagtctttgt ttttgacctc ctgatacatt tgctccacct tcactaatca attcatcaaa
    17701 ctttctaggt ttttttgata taaactcata tgcttgtgca acttttgcgg aatattctaa
    17761 ttctgactct cctgccttat gtttcccaaa tctaatatta tttgctatgc taccagtaaa
    17821 aagcagtgat ttttgaggta tgaatcctat cttttttctt aaagctttta aatcatattc
    17881 tcttatatct actccattta tcttaatgct accttctgtt acatcataaa atctaggtat
    17941 caagttaatt agagtacttt tcccactacc agtacttcct ataaaagcga ctgtttcacc
    18001 tgtttttgca gtaaatgaaa tatccttaag tacagaagct tctccatctg gataggtaaa
    18061 agttacatta tcaaattcta tgataccctt attttcagta tccttaattc cattttcagg
    18121 attttttatc aatggttctt catttaatag ttcttttatt ctatttgcag atacctctgc
    18181 tcttggatac ataataaata ccattgaaaa aagcataatt gaaaacatgg catggaaaag
    18241 atattccaaa aatgcaacca actgtccaac ttgaagctct ccaatattta tcttctcact
    18301 tgaaatccaa aatacagcca aaacagctat atttaatagt aaaaagaatg ctggttgagt
    18361 aattgacata agcttaaata gtttcttaga aacattagca taatatgtat ttgttttttc
    18421 aaatcgctct gtttcataat catcatttcc aaaagctcta attactctta ttccagttaa
    18481 attttctctc gatataaggt ttaatttatc tagtcctttt tgttgttttg aagatattgc
    18541 ctgagatact tttgctatta ttgctactcc aataattata aaaggtactg atatagccaa
    18601 tactatagaa agttccacac ttgttctaat tgtcattatt atacttataa tgaacataac
    18661 tggagttaat aaggcagtcc ttagtagtat attaacaaat tgttgaattt gaaatgcatc
    18721 attcgttgtt cttgtaatca ttgaagaaat tccaaattta ttgtattcag tatgtgaaaa
    18781 ttcttgtgat ttctcaaata tgtcatttct tatatctctg gttacacttg ttgatacctt
    18841 agcagaacaa taacccaaca atatactacc tacaacccct ataatagaaa taataacaat
    18901 cactattccc atttgtttta tgtaagctat attctgattt gcaataccat tgtcaattat
    18961 tttagctatt atagttggaa tccccaactc gactaatgca aaactaaata tagccagtat
    19021 gtttaatact attaataact tataattctt taaataactt aaaattaatt tcatattttt
    19081 ctcctttctt aatttttgag atagtgtaat catgtaataa tactaaattt ataaatgctt
    19141 aattttataa aaaaaggaga aaagagttaa cgcactctct tctccagcat attcttttta
    19201 atacaccctc cgacaagtcg cttatatgtt aacatatttg acaagtaatg tcaattcata
    19261 tagccaaaag tcttttaaat gcttcgtttc ataacattgt aacaaattgg catgtaattt
    19321 atttttgttt tttactgtat tccaatttta caagttctcc aatattacta gctttcttaa
    19381 aattcaaatt agtcttatct ataccagact taaatagtga aatccctctc cccaatatta
    19441 ttgtcaatat atatatataa taatcatcaa ttaagttatc tttcataaat aattttacta
    19501 tatcgcctcc accaactatc caaatatctt ttttatcctt ctcttttcca atttgctcaa
    19561 ttaatttttt aggttctaaa ttaatttata gtgtgattat tctatgtaaa catcactttt
    19621 ttactcattc aataataccc tattaataaa tcctcaaact aatttaaata aactttgtat
    19681 aaaattaaaa atatattata taaataattt aaaactacac taacttattg aaaattctaa
    19741 ttattacaaa taaatattgc tctctgttaa cgctagttct ataatataat tataagctat
    19801 taatattata aagccgatat tactaatata ataattataa tacttatagt taatttactt
    19861 ttgttgaaag gtggtaaata acatgaaaat ttcagaagtc gctactccaa attttttgct
    19921 agatttagac cagttagaaa agaatataga taaaattcaa caaatttgta caaataataa
    19981 taaacaactt tggcctatgc ttaagacaca taaaagtact tacatagcga gacttcaaaa
    20041 aaatgcaggt gctagtggtt ttctagttgg aacacttgat gaaggtgaag ctctgataaa
    20101 agcaggtttt gaagatatta tgttggctta tccttacatg ggagaagtaa atataaatag
    20161 aattgtgact atggcatcaa aaataaattt aatctgtgct acagataata tagaatgtgc
    20221 taaggcttat agtgaagctt tttctaaatc aaaaatagag tgcaagctac ttattattgt
    20281 agattgtggc ttacaccgtt ttggggttga accagaaaaa gtggttgaac ttgctactga
    20341 aatttctaag ctagaaaaca tcaagattgt tggtataagt tcgcatccag gtcaagtgta
    20401 tggttgtagt tctccagaag gtgttccaga tgtttgtgtt caagaagatt cttctatgga
    20461 gaaagcatac aaagctctaa ctgataatgg atttaatata gaaattgtcg cttctggtag
    20521 tactccaaca gttgaatctg aagctgcttc taaaactata actgctgtaa gacctggaaa
    20581 ctatgtatac tatgatgcta tacagatagg tttaggttgt gctactgaag atatgtgtgc
    20641 tcttactgtt gtgtgcactg taatctcaaa aaatagcagt ggatactatt taattgattg
    20701 tggaagtaag tgccttggac ttgataaagg tgcacatgga aatagtagta ttgttggtta
    20761 tggtagggta tgtggacatc cagaacttac tattgattca ctttcagaag aagttggaaa
    20821 aattaaagta aatggtgaaa ctgatataaa aattggtgat agaataagaa ttatacctaa
    20881 tcattcttgt tccactgcaa acttgacaag taatttatta ggttttagag gagatacaat
    20941 agaaaaagtc atagaagtag atattcgtga aaattcaaaa caaatcatcc tttaaaaaca
    21001 catttattct gatattaaca tgaaagtata tttttcttaa aattattgct aataactaat
    21061 gttaatgttg gataaataaa aaacataaat tctagaaata taaatttaaa agaagaggct
    21121 gtctcaaaat agagataagc ctctctttca ttgttgaaat tatgaaaata tacttgattt
    21181 catacaaaaa gagtatctca tgaactaaaa agttcacttt gagatactct ctttttttaa
    21241 ttaatataca cattaattac tatcttagtt tattactata tatcaattac tactcattaa
    21301 tccttgtata tattaattct tatatatcat ttatagttag gttataattt tatgttattt
    21361 acattaatcc cataacagtg agtaaaactc ttactatcaa catccccact attgcatcaa
    21421 atataggaac tgccaacagt aacattctat acttagtact ggcatttgct acaagcacta
    21481 ttctggcata atgaccaact agagtaccca ttaacataca tggcataatt aatatggttg
    21541 cttgtggtgc agtgattaga ccttgtgcaa ataagtttgc agctgtagct gctcctgctg
    21601 ccttagcaaa gaatgctgct atcaaaacta caacagattc cccaggtagt ccaaaaatac
    21661 ccattactgg tccaaaaata tttcctaaaa tatcaactaa accagttaat tgtaaaaact
    21721 gcactattac ataacccatt atcatggctg gtaatatctg ttcaacacct atgtaaaaac
    21781 ctttttttgc tccccccata aatatctcaa cagcagtttt cttaggttta ctatttacaa
    21841 ttacatttcc agtattttgt tgactcatac tacacagcct ccttagcctt attcttttta
    21901 tttcttacac ctatgattaa tctaactaga ttagcaccaa gtattttaca gaatatctct
    21961 ataagtatta ttggacctaa tgccaataat gctattggta aaagtggagc ttgagtattt
    22021 attgtgttca gtataacagc tgaaccagca tattgatatg ctacaaatat agttctttca
    22081 tcatctgtta tttccccact ttcaaataat tctttcgtca ttattgaggc tacatctgaa
    22141 ctagtaaatg aagatacaaa tgctattcct gctattccag gtataccaag cagaggtcta
    22201 agcaatggat taaatatctt tgatgccgct ttcattcctc ccatagattc aactacatct
    22261 ataagaccta cagcaaaaca gacagttgga atcaaagtca gagcaaataa gaaaccatct
    22321 tttgctcccg ttcctccttt acccataaag tctacaccat ctgcaatagt accaaatgaa
    22381 cctgtcagat tagcaaaatc taatgcttta aggaagcctt gactatcttt aaacactcct
    22441 gaaaataata ctatcaatac aaataaagat aaataccctt tccatgttac cttattatct
    22501 ccagctgtta catccatata aaatccccct ttatacctca aatttatcta agtttaaaat
    22561 aactgtagaa attaatttca ccctctcaaa catagaatct actactgcat attctcttga
    22621 tgtgtggttc cattctcctc ttacaccaaa tgaacagatt gtaggaacgt ttgcaatagt
    22681 taaataactt gcatctgaac tacctccaag tctcttagca tttaattctc caaatccatt
    22741 ttcttttgac acttcattta caaatgtatg aaatctcata acatcttctg tagtttcaaa
    22801 tggcatcatt tcacttataa aattaacttc tgtagaagtt ccttctatgt aagtttcttt
    22861 acatatctct ctaacttgtt ctttaacatt ctccatttct tctacttttt caaatctaag
    22921 gtctactttt atactacaat cttctggaat agaattttcc actcttccac ccttaatcac
    22981 acttacactt actgtagttc caacatctaa attagtcaat ttttgtatgc gtagtatctt
    23041 attagccatt tcttctatag catttcttcc accttcaaaa tcattacctg cgtgtgtttc
    23101 tacacctttt acatgtatat tacatcctat tcttcctttt ctacctacac acaaactatt
    23161 atctactaat ccagtttcca tattaaaagc acaaagacca ccttttgctt ccctcaatat
    23221 aacatctgca cctgttgaat ccttatgtcc tatctcttca tctccagaaa atactatttt
    23281 tattggtctt tctttgtatc ctattttatt taatgcttta atagcatata aggaaattat
    23341 tataccaccc ttcatatcta aaacacctgg gccatatgct tttccttcta ttattttaaa
    23401 tggattctct ccaaatgtac ctgtttcaaa gacagtatcc atgtgacctg aaaaaattat
    23461 tggcttttta tctatattgg aaccaagtgt acctatcaat gtactaccat tatctccaac
    23521 atctactaac tcgcaatcta aaccttcttt ttcaaactca aactttaatc tcttagccaa
    23581 tttatttacg ctttctttac aatgtgtata gctttccata ttaacaatct ctttccataa
    23641 tgaaactatt tcttctctat tctcatcaac aaacttagaa atttcattta aatatgtatt
    23701 cataatcttc tcctttattt tcaaataaaa tgtatttctt tcctaaaatc taagtttatt
    23761 ttaggaaatc ttaaaagact agtccttaat tacaaaatag cacaattatt cttgacagtc
    23821 aacatatttt tcagtctttt ttatattttc tgatttttct ttcaattttc cttttgtaaa
    23881 ataaattgac gcgttttatc tactattata aaatgaggtt ataaattgat tgattagtta
    23941 caatactctc gaaattttaa aggaggtttt atgaaatata aaattggttt tttgacaaca
    24001 tcttatttta tgtcagataa gtttaaacaa gctcttaaag aattaaatga tacttgtgaa
    24061 attacattta ttgagcttaa aggggagaat ccttttaata atttacctaa tatatactat
    24121 gagcaagtag ataattttga tggatttgta tgcagtggta tagttccata tagtgaactt
    24181 ataataaata taaaagacat aaaagttcca ttaaattttt taaagcttga tgaaaaagat
    24241 ttttataaat acttattcaa attattaaat agccaaaaag atattgactt tacaaaatca
    24301 tttatggatt ttttaagaga ggacaacaat tattatgata tatattcatt aatagactct
    24361 aataaatgtc catatacaat taaagatttt aatatccctc aacctgtata tgactttaaa
    24421 aaattaaatg ataaattatt ggaaatacat ttagacttat tgcgagataa taagattaac
    24481 ctttctttta ctagaaatta catcattagt tgtgaactta ataaacttgg atatgattct
    24541 atttacatgg ttccatctac agaatcaatc ctgaatacat ttaaatctct ggtgaatgaa
    24601 ataacattac aaaacttaga caaaaataaa tctgcaactt gtatagttac agtaaatagt
    24661 tcagagtaca taaatgaaga tttgatattt aaaaatgatg agatacaaaa tcaaatatat
    24721 aacactatat taaattctct atcaagacat ggattctatg gagttcaggt caaaaaaaat
    24781 gatatgaaaa tagagataca cactactaaa gaaatgttaa acaatatgac caataactat
    24841 acagattttt ttatttcaga agatttaaaa gaaattaaat atagcttaaa tataggttgg
    24901 ggcattggaa atacaaatat tcatgcagag caaaatgctc gtcaagctaa tggtaaatct
    24961 gcttctttga atggaaactg tacttttata gtaaatgata ctaatgatac aataggccct
    25021 ttatattcaa ataaaaattc taataatatt gaagatagct caatagccaa taaagttgct
    25081 tcttttatac ctctatctaa tacaaatgta tctaaaatca tgtgtatgat taatgataga
    25141 aattcaaata atgtgtcagc agaaatatta gcagactata tgaacataac tcttagaagt
    25201 gctaatagaa tattgagtat cttatataaa gcaggaattg caactattgt aaatacaaaa
    25261 ctcgataatc aaagaggaag gccaaaaaaa atatataaag ttgatttctt atcttttcta
    25321 aataagatgc agaaattaaa ttcttaatat ttaatttttt aataaaagca atgtattttc
    25381 tctaatttta aagaatcaaa atgtttaaat tttattagaa tatacaactt tttactttaa
    25441 aatacctaaa ccttttatca gtgaaataaa ccataatata tcgtaactta ttagaaaaac
    25501 aaaataattt catatacaaa taataaaata gccactacaa tccataattt gcagtggcta
    25561 ctttttacac tatcacttat tacttatttt taaaaattaa ttgtattttt taaataattt
    25621 atacacacct attatcagta aagcaataaa tacaagtata tctataaaaa taattattgt
    25681 aacatgtctg acaatattgt aaataaaaaa tatagagaat atttctatag acaacgctac
    25741 aaaacacata aaattcgtta aataatctgc aatagtatca ctataaaaag catgtatttg
    25801 taaccatctc catataaatg gacgtaaaag tattctgaat acaacacaaa tgacagggta
    25861 ggcataaata aattttttat ctacataatt agctacacca ttattccatt ggataggaat
    25921 ttgaggtggc aacttttgaa atactgttat tgcaatttaa acactacctc tctattctat
    25981 taatttataa ctataatata gtcttaaata ttttcactct ataaaattgt atatttagta
    26041 tatcttaaaa tcaaactaat tatcttttat aagttttcat acaataatat ctcc
//
LOCUS       DAMJZE010000034        25051 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJZE010000034 DAMJZE010000000
VERSION     DAMJZE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.034"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            104..778
                     /locus_tag="PC541_001324"
     CDS             104..778
                     /locus_tag="PC541_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838794.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            766..1926
                     /locus_tag="PC541_001325"
     CDS             766..1926
                     /locus_tag="PC541_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2838795.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2142..2669
                     /locus_tag="PC541_001326"
     CDS             2142..2669
                     /locus_tag="PC541_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2838796.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            2955..3623
                     /locus_tag="PC541_001327"
     CDS             2955..3623
                     /locus_tag="PC541_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838797.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3626..6094
                     /locus_tag="PC541_001328"
     CDS             3626..6094
                     /locus_tag="PC541_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838798.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6334..7758)
                     /locus_tag="PC541_001329"
     CDS             complement(6334..7758)
                     /locus_tag="PC541_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838799.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7777..8469)
                     /locus_tag="PC541_001330"
     CDS             complement(7777..8469)
                     /locus_tag="PC541_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838800.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8521..9300)
                     /locus_tag="PC541_001331"
     CDS             complement(8521..9300)
                     /locus_tag="PC541_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2838801.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9297..10067)
                     /locus_tag="PC541_001332"
     CDS             complement(9297..10067)
                     /locus_tag="PC541_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2838802.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10064..10768)
                     /locus_tag="PC541_001333"
     CDS             complement(10064..10768)
                     /locus_tag="PC541_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2838803.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11023..11535)
                     /locus_tag="PC541_001334"
     CDS             complement(11023..11535)
                     /locus_tag="PC541_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838804.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11861..12118
                     /locus_tag="PC541_001335"
     CDS             11861..12118
                     /locus_tag="PC541_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838805.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12261..12887
                     /locus_tag="PC541_001336"
     CDS             12261..12887
                     /locus_tag="PC541_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2838806.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13134..13913)
                     /locus_tag="PC541_001337"
     CDS             complement(13134..13913)
                     /locus_tag="PC541_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2838807.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(13981..14442)
                     /locus_tag="PC541_001338"
     CDS             complement(13981..14442)
                     /locus_tag="PC541_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2838808.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14418..15095)
                     /locus_tag="PC541_001339"
     CDS             complement(14418..15095)
                     /locus_tag="PC541_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2838809.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15328..15714
                     /locus_tag="PC541_001340"
     CDS             15328..15714
                     /locus_tag="PC541_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2838810.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15717..17516
                     /locus_tag="PC541_001341"
     CDS             15717..17516
                     /locus_tag="PC541_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2838811.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17871..19745)
                     /locus_tag="PC541_001342"
     CDS             complement(17871..19745)
                     /locus_tag="PC541_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2838812.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19750..21414)
                     /locus_tag="PC541_001343"
     CDS             complement(19750..21414)
                     /locus_tag="PC541_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2838813.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21428..22249)
                     /locus_tag="PC541_001344"
     CDS             complement(21428..22249)
                     /locus_tag="PC541_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2838814.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22348..23112)
                     /locus_tag="PC541_001345"
     CDS             complement(22348..23112)
                     /locus_tag="PC541_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2838815.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23109..23858)
                     /locus_tag="PC541_001346"
     CDS             complement(23109..23858)
                     /locus_tag="PC541_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2838816.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24362..24967)
                     /locus_tag="PC541_001347"
     CDS             complement(24362..24967)
                     /locus_tag="PC541_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2838817.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         8970 a         4033 c         3060 g         8988 t
ORIGIN      
        1 aatgattaag ttttgaaata taactaagaa tttgataatt tattgagatt tttatactaa
       61 aatatacata ctatacaaac ttaatttaga agggagcatt cttatgaaca ataaaatact
      121 cgtcgtagat gatgaggaag gtataataac actcttaaaa gattactttg aaatgaatgg
      181 ttatgaggtt tacactgctc aaagtggaaa agacgcatta aaaaaaatat ctaaaaatcc
      241 agatatcatt ctacttgata taaacatgcc tgaaatagat ggaattgaag tttgtaatag
      301 aattagagat tatgtatcat gccctattct atttcttaca gctagaattg aaaatactga
      361 caagataaat ggttttcgtg ctggtgctga tgattatatt gtaaaacctt ttgatttaga
      421 cgaacttgga gctagagtat ctgcccattt aagacgtgaa caaaggaagt caaatcaaac
      481 taaaattaaa ttttataatg atttaacaat agattacgaa aaaaggttaa tttgtatata
      541 taataatcag atttcacttt caaaaaagga atttgatata gtagagcttc tctctatgaa
      601 tcctggacaa gtatttgata aagaaaaaat atatgataga gtctggggat acaatagcaa
      661 tggaaatagt gatgttataa tggaacacat tagaaaaata cgaatcaaac tatcaaaaca
      721 tactcttgaa aattatatag aaacagtctg gggagttggt tataaatgga taaaataaat
      781 aatatgtcat taaaaaaagc tttctttgtt cttactttat cttcactaat tatagcatca
      841 gtgcttacta ctttcgcata cattttactt aataatctat ataattctat acaagataag
      901 tatttaaaaa cacctataaa tacaggtatt ataaccatag ttcaaaacaa tgtgaaccaa
      961 tttagtgatt atgataaaac attacttaat ataatcaaca tacttcagct tgtacttcca
     1021 cttatctttt ttataggact tttattatta gctgatataa ttttttacaa ggtaaaatta
     1081 aaaacaccca tagaaatact caataaaggt gcaatggaaa tatccaacaa caatttagac
     1141 ttttgcttag aatataataa taatgatgaa ttaggtaatc tatgtaatgc ttttgaaaaa
     1201 atgaggtctg aactaaataa aaacaacata aagatgtgga ctatgattaa tgatagaaaa
     1261 caacttaatg ctgctttttc tcatgattta agaaatcccc taacagttct aaaaggttac
     1321 tcaaactatt taacaaaata cataccaaca ggtaagctta gtgatgagaa gattttatca
     1381 accacacaac ttatgtctga gcatattaac agaattgaat actatgtaga aaatatgagt
     1441 aatgctcaac gattagaaga tttagttgtt tcaaaatcta tgtcgaatat aaacaagttt
     1501 atagaaaact tagatgaaaa tatatctatc attgctaaac aagaaggaaa atcttttaca
     1561 cttaaaagtc aaataaataa tataaatcta ttttttgatg aaaatatcat tcttagagtc
     1621 gtcgaaaata taatatcgaa tgcttttaga tatgctagaa ataatgtatc catacttata
     1681 tatcttgaac aagaactcct tacctttgta attgaagatg atggtatagg atttagtaag
     1741 gaatctctaa aattagcttt aagacctttc tatagagata agactttaaa tgacagcaat
     1801 gcacactttg gtatgggtct ttatatctca aaaatattat gcgaaaaaca tggtggctct
     1861 atttctattg aaaataattc tactaacagt gctaaaatta ctgctaaatt ctcaacaaga
     1921 aattaaaatg attttgtaag ctttcactgt tcttatataa aatttgttag tttagaattt
     1981 ataaaatgta tttcaattta caaccaatat atatactatt ttgtagaagt tgtaagagtt
     2041 gcttcaatta taatatttgg caaaaggcat ttgaaataat aatagaaata tatatttaaa
     2101 tatgtagaat agtatgaaat tagaattttg gaggtgatta tattaaaagt tttgctaata
     2161 atattacatt ctctcgtatt gtgtttgcat tgctaatgct tagttctaca ttctcttcac
     2221 tgcctttctg gatatgttat ttttggtgtg gaattagtga tatacttgat ggtcttattg
     2281 cccgaaagtt gagacaacaa agtggaatag gtgcaaagtt agatagtatt gctgatttca
     2341 tttttgcggt tacaattttg gttactgtcg gaaaaaatat tgctcttccc atgtatctat
     2401 ggtggtgtat tgttgcaata gcattgttac ggttgattag ttacagtatt ggttttttta
     2461 agtatcgcac attttcatcg cttcatactt tgatgaataa aatcactgga atttctatct
     2521 ttgcttttcc attactctat atttgctttg gtcttgatgt tgcaggaatt atgatatgta
     2581 tcgtggcttt tctttcttct tttgaagaaa tagcaattat gataaaatca aaagaattaa
     2641 atcgtgatat aaaaagtata tttatgtgac aacttagaat ttgtgacaaa aataatttat
     2701 ataataatga ggaagtaaaa cataaaaaat tatccacaag tttatgtgta aaatagtttt
     2761 aaagcaatag agttgtattg taaagccttt agtgcataaa ttacatttta aattaaaaat
     2821 gatactgaaa ctgcttatca gcactgcgaa ttatccgcaa atagtgaaga ctttttagat
     2881 ttaagctaaa agttgggaaa aaattgagtt ttaaaactta cactcttctt atatagaaaa
     2941 ggagagtgtt ttttatgatt ataaaagcaa aacaattatc aaaaatatat ggttctaata
     3001 acaataaagt tatagcactt aataatgtaa atttagaaat aaactcaggt gaatttgttt
     3061 ctgtaattgg accttctgga agtggaaaaa gcacactttt gcatatttta agtggattag
     3121 ataatcctac ctctggacaa gtgttacttg atgataaaga tatatataaa cacactgaaa
     3181 aagaactatc tgcacttaga agaaaatcct ttggattcgt ctttcaacaa tttaatcttt
     3241 tacctgttct tacagcatct gaaaatattt caatgcctgt acttctagat aaaaaacaac
     3301 ctgataaaga ctatttaaat gaaatttcat cattacttgg aattgcagat agactcaatc
     3361 atcttcccca tgaactttca ggcggtcagc aacaaagagt tgcaattgct agagcactta
     3421 ttgcaaaacc agatataata tttgctgatg agcctacagg aaacttggac agtaaaagtg
     3481 gcagtgaagt tatgaatctt cttataaaaa cttcaaaaca gtttggaaag actttagtgg
     3541 taattactca tgatgataga attgcaaaac ttgctgatag aaaaatttca attattgatg
     3601 gtgttcttat ggaggtgaaa tagaaatgaa gaattacctt tcactttcaa taaaagaatt
     3661 aaaaagccag aaatttatga ctacattcat cattatagca atagtactat caactattat
     3721 gacaactgtg gttggacagt caattggtat cctacaaaat ttaagaattg aacaagcccg
     3781 ttcttttaat ggtgatagac atgtatcctt tcaccaactc actaaaaatc aagttgatga
     3841 cataaagaaa gatgatagag tttatcaagc tggtacttca ataactattg gcagtagtaa
     3901 aataaaggat tctggtatct ctgtacttgt aaaagagtat gataaaacag gactttctaa
     3961 ctacccaaaa ttaatgaaat taaaatctgg acatttgcct aaagataaaa acgaaatagc
     4021 cttagatgaa aacacactaa agcttatggg tataaaacca agactaggag tgactatacc
     4081 tatgaattta gatatttcac ttttaaatga taccatacca ccatataatt acacagctaa
     4141 ctttaaattg agtggcattt tagaggatga ttatacagga tatgtaagtg gtatagtaaa
     4201 tggtattgta ggtaaaggta catcagaaaa tctattacct aagagatata ttttatcctc
     4261 tttggatttt aaaataaaac agcaagaaaa gtttcaagaa atagtaaatc aattagctaa
     4321 aaaaataaat ctttctcaca attcaattca atataattgg atttacttaa atgctcttaa
     4381 gatacagttt gaaaaagatg aaaatagttc taatagtgat ggtatatcta tgattatatt
     4441 ggtatcttta tttgttgcct tattggtttt actggcttct ggacttgtaa tttataatat
     4501 tttaaaaatc tctgttacaa agaaaattaa agagtatgga tgtttaagag caataggagc
     4561 tgaaccaaac cagatatata agatagttat tttacaaatc ttaattttat gtactgttgc
     4621 catacctatt ggagctgtaa ttggaattat atcttctaaa ggcataactg gaatggtcac
     4681 taatatctta aatccagata tacttctagc aaatgataat aaagaaatta ctgagcttat
     4741 acataaaaat acaacagcat atatattccc attagtatta agcactaatg tatctttgat
     4801 tttttcattt atttcagcat taccatcagc aatatatgct tcacatgtct ctcccaaaat
     4861 tgctatggtt ggtagtacta ccaaaatcaa aagaaaaatt aaaagagaaa aaacaataaa
     4921 aaactttgaa agacatctag catggctaaa cttaaaaaga aataaaggaa gaactataat
     4981 aactatacta tcattattta tgagtataac agtattcgtt gcactgagtg agttctctaa
     5041 tgtattagat gtatctaggt cagtaagtaa tttaaaagaa ggagattttt ccttaacaaa
     5101 tgaaatatct ggttttgata aatcaacact agataaaata gctaaaatga aaaacgtcaa
     5161 tcgcacttcc tttataaagt actctgaata caaacagggt gaaatagata cagatataaa
     5221 ttttgaaaat agtggtgaga tgttaaaaat cattggaatt gatgagcaaa ctttaaaaga
     5281 cttaatgcct tctattacag attctatact agaagacttt aaaaatggtt ctatctgttt
     5341 tataaaaaat ccgattgcta tttctacacc aggtgtaaaa actagatata caaatttaaa
     5401 acctaaagat agtatcacaa tcaacaaaaa acaactagat atatatagta cagtagataa
     5461 aatgttcttt ttacaaggca atggctggat aaatggtgtt gatgttattg tatatgattc
     5521 tgtttataac actttgacaa ataaaaacaa gttcaaccag ataaatattt atgctaaaga
     5581 taaatcaaaa ttagaacaaa taagattgtc tattgaacag atttgtgaaa ataatcctgg
     5641 ctctcattgg atatcatata ttgaatcaga taaacaactt aaagagagtt ttaaacaaat
     5701 agaatttcta gcatgggcag ttatattatt tgtgggattg attggtactt taaacatcat
     5761 taacacaact catacaaata taaatactag gactaatgag attggagtta aaagagctat
     5821 tggtatgagt aataggagct tatataaaat gtttttatgg gaaggtgttt attatggaat
     5881 ttttgctgct atatttggtt caattgctgg gtatgccagt gctattatca taaacatggc
     5941 aactatagaa aagcttgatt tcactaatat accaattact tcaattttac aagctactat
     6001 tatttctgta cttgcatgta ttatagctac tcttattccg ttaagaaaag ttaaaaagat
     6061 gaatataatt gactgtatag atatcaattt ataactattt tacagataat aatataaatt
     6121 aaattatcaa tgactgtctc tattttcttt caaatctaac taataaatct ttttaactat
     6181 aatataagct ttaaaaaagg cctgtctcaa aatagagata agcctttttt cattgtcgaa
     6241 ataatgaaaa tatatttaac ttcacaaaaa aagagtgcct cacgaattaa aaatttcgct
     6301 ttgagacact ctcctttaat ttaaaatata tagttaaatg ctatgccaaa atttcacaca
     6361 tgctccacct gattttgaat tttctatttt aacagaacct ccaagctgtt ccactaattg
     6421 tttgacaatg taaagcccaa gaccagaatg tccacctttt gtctgcctag cttcatctcc
     6481 tcgataaaat ttatcaagag ccttttttaa atctttagag ctaaaccctc taccagaatc
     6541 acaaatctca taagaaatac aatttttttc atcctttaca gtaatactaa tattaccacc
     6601 tgatggagtg tattgcagac tgttggatat gatattatca aaaattcgtg tcaacctatc
     6661 aacgtcgatt tgaattgggg attgtatatt cccctgcatt ttcaatataa gttcaacttc
     6721 cttttgatga gcctgtaatt catagtcttg caccttttgc ctcaaaaatt caggtaaatt
     6781 tataggaaca agatttaatt gtatatttga cttttctaag tcagacgtat attgcatctt
     6841 ttggacaaga gcagcacttt tttctgtatt ttctcttatc actgtaagat acctatgaag
     6901 tttttcattg tcatctgtat tattaccaat aagtgcatct gtatatccca aaataataga
     6961 aagaggagat tttaaatcat gtgctagagc ttccaccatt tcaacacgtt cctgctccat
     7021 cttccactgt gcagataaag agccttttaa ttcgtctttc atttcagaaa aggcactaca
     7081 aagtttaccg agttcattat ctgaataata gtcgatttca aaatcaaggt ccttttcttt
     7141 tatcttcttg gacgcatcaa tcaatagatg taatggttga ttgatattct ttacaaagcg
     7201 cttagaaagc catctagtaa aacccacgat atatagaaat ggagaaaaca aagccattat
     7261 aataactgca aatacaaatc tccctctaat atttgcaaag gtgattttca cttggtagaa
     7321 tagaataaca gcaccctcaa tctttccatt atcccctttg attggaacag tatggatata
     7381 accaccttta cgaacagttt tattgataaa attattaaat aattcttctt ttgttttaaa
     7441 tagttttttg gggttagtac catataaaat attgccatta ttatccacaa cttgatatag
     7501 catatcatcc ccacgaatcg ttctttttaa accttcttca ttcgattgtg ataacaaagc
     7561 tatgtttttt tcattgatgt atttttcaat tctaggaact tgtcgctcat aatagtttgg
     7621 tggatagata tccttattta aggaatgaat atacaaatag agagcaaata cataagtaat
     7681 cactgtggca actatactgg ctattataat aaaaataaaa gtatgtctaa attgggtaat
     7741 aagggattga tttttcattt aataacctct ctctctttac attttattcc acttataccc
     7801 aataccccaa actgtggaaa tatattcttt ttctggtgtc acagcagaaa acttagctcg
     7861 tatttttttt actcgttcaa caacagttga ggaatcacct tcagaatcac acccccatac
     7921 tttttcatag atttgttctc ttgagaaaac ttgtcctgca tggagtgcta gtaattcaac
     7981 aatatcatac tctatttttg ttaaatcaat attcttacca tcaattttaa ttgcacgttc
     8041 cttcatatat aagcataact tcccaaaata caaagtagat ctcttgttct cttcattgat
     8101 atactgtgaa cgcttttccc tacgcagatt agcttcaatc cttgccataa gttcacgcag
     8161 tccaaatggc tttgttacat aatcatcccc tccaaaggtc aaccctttga ttttgtcggt
     8221 ttctgattgc tttgcactta aaaatataat tgggcataaa acatcatctc taattgcctg
     8281 acatacctca aatccattca ttttaggcat cataatatcc agtataataa ggtcaggctc
     8341 ctgttttgat aactctatac ctgcctgacc atcataagct atcaacactt catgtccttt
     8401 tgccttcaat tcatcctcaa gtagcattac taggtccatt tcatcatcaa taatcaatat
     8461 tttactcaat ataacaccac ctttctctta tgggaaggtc agtattaaaa caatattcgt
     8521 ctactcataa cttttccttc cctcccacat tttaaaccaa ataacgcctc ctatcaagaa
     8581 tataatcaaa cctattgcta ctactataag ctctatacta gctgtttgca tcactttaga
     8641 gtaaaatgcc tcagttgaaa tagttccagc ttctttaata aaataaattc ctggaagcat
     8701 acctagtttt actggccatg tccatggaac aaatgcccat actttttcac caacgctagt
     8761 taatccaagt attgctgcca tcaaaattcc accaatacta ataccaatag atgcacccat
     8821 accccatgca aaactcaccc aaagatgaat agctaatagt ggaagtgtcc caatcaccac
     8881 aagtcctgct gctgacagga ataaccatat tgttgctcca gatggcactg caatactcat
     8941 tcctatgcaa agtatcaacg tagcaataaa tgtgcatata gttaggcaaa acagaagaaa
     9001 aagaaatttt cccagatata atctgacacg agaaatacct acacataaaa aaccattaaa
     9061 atttccagca agctcttcct cctgcactat aaatcctgct aaaataccta ctccaatagg
     9121 aatgatgaat acagtccata ctgtaaaaaa tccttcaaaa gcaaattctt gtgtacttcc
     9181 agatttagtt gtgagataaa aaaccacaca taatgaaaaa ataataggca tacaaagagt
     9241 aagccactgg acagctgtcc tctttgctcg aagccattct gatgataata gttttatcat
     9301 ttgtcatccc tcctattgaa ccaagctgca gtaagtgctg ttaatactat aaacacaacc
     9361 aaagaaacaa caatcccaat aggaatcact gaagtattca aaagtgggtc tccagcttct
     9421 aatatagtac cgttaggatg tatgttgatg attgggcata ccattctaac tgcccaactc
     9481 caaggtactg caatccaaaa tggttttgtc gcagcaagaa caccaaatat catacctaaa
     9541 gctccaactc ccatacttag gaacatacct ttccatgtag ctagccatag ctgaagtgga
     9601 agtatagcaa gagaaacaac ccagcataca atacttgccc ctataatctg tccaaaagga
     9661 actggaccag ctttcaatat taaaccagtt ataattgtta cgactattaa aactagcgtt
     9721 gaaataaaac tgtagactgc catagctata actttattaa tccaaagtgt cataggagac
     9781 acattgtgtg tacgtaaagc acgataattt ccagcttttt tttcctggaa agctaccaaa
     9841 gtagcgaaca atgcaaatcc aataggcaga aataaaaatg accaccagtt aaaaattaat
     9901 gctaaaagat tttgccacgc ccaagaagtt gtttgagata atggattttg catgagtgtt
     9961 gattgcatta taaatgcata tgctgcaaaa gacactggga aaaaaacaat aagtttaccc
    10021 ataaatgttc ttttatactt taaaagttct gattgtaaag cattcatctg ttttgtcctc
    10081 ctttaagaat aacatctgta aaaaattctt ctagattttc attagggtca ggcattcctt
    10141 gaaataatag tcgtcctcca tttataattc ctatattatc tacaacttga gcaacctctg
    10201 atagaatatg gctagaaaga attacagtta tacctttttt gggaaaagaa gcaatcaatt
    10261 cacgcaattc ttgaatgcca aaggggtcaa gaccattggt tggttcatct agtattaaca
    10321 actctgggtc actcaaaagt gcaatagcta ttcctaacct ttgtttcata cccatagaaa
    10381 attgtgaagc tctcttttta cctgtatttt tcaaatttac tatttccaac acttcataaa
    10441 ttttatcttt aggtattccc aatatttttg tatgtactaa caaattttcc tctgctgtaa
    10501 ggtttccata caatgcaggg gattctatta aagaacctat ttttgcaaga gattctcgtt
    10561 tccaaggctt tctatcaaaa ataatacgac catttgttgg gcgaagaaga ccaacaagca
    10621 ttttaagtgt tgtggacttt cctgctccat ttggtcctaa aagtccatat atactatttc
    10681 gttcaatttg tagagaaaca ttgttaactg ccatttgttc tccatatctt tttgataagt
    10741 tttttgtttc aagaattaaa tctttcatat taatttctcc cttcttgtaa taccttaaat
    10801 ctgttactaa gagaatacta acatgaaaaa ataacaattc tataacacct actaatttta
    10861 ataaaattta aaataaaaaa tagagttacc aattttattt tggcaactct atttaagttt
    10921 atgttttaca gttatattag atttaaactt agtttcatta tcacactaac ttcaaattta
    10981 ctctataatc tttaaatcaa acactaattt attaatcaaa cactaatcta ttaatttata
    11041 tttctttaga catctaatat agtttgctac taaataataa ttatttatat catatttatc
    11101 taatccttta aaagaagtta tttcatcatt aagctcatca ctaccttcta caaaaacaca
    11161 acctgtttca aattccatac ctacaataat cttacagtca ctataaattt cctcttgagt
    11221 atcgtacaat ttttttgcat ttccttcatt tggaattgta aatacaatag gttttaattc
    11281 tttatcactg gaaatctttt gtgaaaaagg cttgtttaac ttaatattat tgataactac
    11341 atgtttaaat tcctctgaag tttcttctac tattacatat tttcgcttta aaaactctat
    11401 tatttctaaa acagacttag aatttacttt taaaatactt ttatattttt catataaaga
    11461 tttccactgt tcaatttttt cctgtggtat ttcttggata tgtgtactta ttttttcttg
    11521 agatattttt ttcaattaaa ctctcctttc aatacttaaa tatccttatg aatctcacat
    11581 gactaaagtt acaaggtttc tacttaatag aattttgcat acttaaatat gtcttattaa
    11641 ttctctacag gatatctccg tagttcctac gattcttaca taagttactt attttaaatt
    11701 gttattagtt atattctaaa aattatttat tttatcacta atataatact cttaaaaact
    11761 ttacaaatca atttaaattt gctacctttt atttataaca acttatagta catttctcct
    11821 atgcttttca atatattact tttaaaagga ggttttcatt atgattttaa atagagctga
    11881 gcttttccga ggatattctt taaatagatt taacgagata tgcaatatat tatcagtaaa
    11941 taaaattaaa tataagtata aagtgaaaaa taatgcttac tctaaaaaac ctttttttaa
    12001 tgaaataaca cttggcaatt taggccaaaa ggaagatttc tcttatgagt acttaattta
    12061 tgttcataaa gacgattatg aacaggcaga gtctctaata aattctaagt tgcgatagat
    12121 tatctataaa acatgacatt ctgttgtcat gttttatatg ttattatact ttcaaatatg
    12181 ctactatatt ttcaaaattg tgtgctttaa aactagcttt ttttagtaat ttaaagctta
    12241 aaactaagga gactcgttat atgaaaataa atataaaatc aaagttaagt gaatttatta
    12301 aacagaacaa tctttttgat gacagcagaa taatatccta tcttcctaat ataacaatag
    12361 aaacagacaa aataaaaact ataatgatta atgaggttgt cccaactaac accaatgacg
    12421 atttttatag tgtagataaa gatgctgatt atttaaaaac tactattcct ttatttgaca
    12481 gtgcaggaat aaaagtatct aacataaatg atatcctaga tatgggtatc tatataacta
    12541 atgcagtaaa acttcctaaa tcagaatata caataactcg tgatacaatt aaattacata
    12601 tgcctatttt agaagaagaa attaaattat ttaaaaacct agaggtagta atgttaatgg
    12661 gagatgttgc aaaaaaatct tttaatatga ttactaaaaa gcatataaaa aagaatgtca
    12721 tcccttccat ttcaacatat aaacttcgta ataatgaact ttattatgat aatttaagag
    12781 tatttccatc atatattatg actggtggaa acatactgat tgaaaaatca aaatttgaaa
    12841 tggcatcaga agatataaaa aaaatgttcg agattataaa cggttgatat tacaaaataa
    12901 taaaaactaa tataaaataa aaagtgcctc aaaaacttct atttatgagg cacttttatt
    12961 ttatattagt gtaactatat actattaaca taatttatta tttatattgt tctttttaat
    13021 ctctattcca tttagtctag gtaacattac tctttacaag attactttat atttctttta
    13081 tatctctaac gtttttattt aataaaacaa ttattaatac aagtcaaata tatctattta
    13141 tctaaaaata tatcttttac agtaatatct ggatggtgat acttcttcct attcgttaaa
    13201 aaagattttt ttacattaat agcctctttt ggacaccagt gaaaacaagc taggcatctt
    13261 tcacactcat taccccactt tggcttttta tcaactatac ttatattctc tacaggacaa
    13321 acccttttac atgtcccaca tccattacat ttattttctg caaaaaattt agtagttgca
    13381 atttgcatat tcttacctac taaacgaagt acaacactat ctatatgtga ctttaaattg
    13441 ttttttcctt ctatatttcc aatctccaag ttattgatac attttgcaat ctcagctacc
    13501 ctattagtct gatttttcaa tctttcttct tcaacatctg gaggtgttat caaagcattt
    13561 ccaggcatat tgattaaaaa accagcagat aattttttcc cccttttttt aagtaactta
    13621 tctatagtaa ataaactatg tccagctacc ccattacaag tggcaatggc atatatatga
    13681 gtattaatat taaaatttaa tttctctaca aaagacttta ctatatcagg aacatcagca
    13741 aaataaacag gaaaaacaat taccaatata tcttcattta tgtctatatt attttctttt
    13801 ctatgtatag ctaagggtat aacttcttta cttccattta acgtttcttt taaaatattt
    13861 gaaactacta gcgaatttcc tgttccagaa aaataataaa tcttactatt cattcttatc
    13921 cctctttcta tatttctaca tcatcaatag ttaaaaagtc tcttaaaata ggatttatat
    13981 ttactttcca attctatcca aaatatcagt aaacaaaggc aattcttcat cagagataga
    14041 tttaaatgtc tctgttgcat atatacttgc aatttcattt aatggttctt ctaattctct
    14101 acctaaatct gttaggaata tttgaaactt acgtctatct ttaacatcta ctttacggac
    14161 aattaatccc ttatttttca taacatctaa aattcttgtt gtattagatt tatccttaca
    14221 tgatttttca cataaatcta tctgagaaat cccatcttgc tcccatagaa accttaataa
    14281 cgcataatgg tcagttgtta tatcatatcc attttgttta aacatctttt ttaaatcatc
    14341 ttttactttt aacgaagtac ttactaactt agttgtagct tcatatagtt tctcaaaatc
    14401 tttcatttta cctccattta aataatccct tgctcctccc atctatcaat tattccctta
    14461 tattgctcaa tatctttaaa agccattggc tttaaaacag catttatagc aataaaatta
    14521 tagatttgag aaagcacagg tggatttttc ttttcctcta tgcaataaat aaatttttta
    14581 gatacctttt gtatctttga ttctactttt tcagttatag gattttgatt aaatccagtt
    14641 tttatagata aactaccaca aacataacct cccgaatcta agaccatgga ttttataaaa
    14701 gaaattgctt ttgaaccacg tgcattttca tatgtagtaa ctgctataca tggcttacga
    14761 tacaataatc tttcaagcat catatgtgct ctatctgtaa acaccttgaa aagtccagtt
    14821 acactacttc cataagttgg gctaccaaat ataacaccat cactcttagt aattatatca
    14881 tgaatatact ctactttatc attttgattg atacacttgc ccattttata acaatttaaa
    14941 caaccaatac aatatgatgg atttacctca cttaaatcaa aataattaat ttttacatct
    15001 ggtattaatc tttctatatc tttatacaag taagttaaaa cttttgatgt agctccattt
    15061 tttctagggc ttccatttat aactgtaatt atcatttcta tatctccttt ttagttgtta
    15121 tatcaacaat aagtatatca actataattt tagctgtcaa tataaaccat ttatttaaag
    15181 ctatacttac acagcttaaa taaaagtttt atttaactac atgtgcttat ttaatcaact
    15241 ataaatgaat tatttttaat aattgacaaa ctaaattaat gataatatac tttatgtatt
    15301 acaaatgtaa gtagaaggag gtatagtatg aaaaagttac cacatatttc agaagcagaa
    15361 tatcaagtta tgaaaattat ttggaaatat gctcctatca gcactactga agtaattgaa
    15421 aagttggtag aaacaagtac atggagtcct aaaacaattc aaacaatgtt attgagatta
    15481 gttaaaaaag gagctttaac atatgaaaaa aatagcagag tattcgttta tactccactt
    15541 gttaaagaag aagaatacgt agctacagaa agtagttctt ttttaaatcg tttttataat
    15601 ggtactctta attctatggt actaaacttt ttagaaaatg ataaactatc tgaagatgat
    15661 attgaggaac tgagagaaat cttaaataaa aggacgacta agggggataa ataaatatgt
    15721 atgccccatt ctttatacga tttttactca gtacattaac cttatcaata ttaattgctg
    15781 taattttact tgccaaaaaa gtatttaaaa aacatatttc tgcaaagtac caatacaata
    15841 ttggatttat attgcttata atgttaataa ttccatttgt ccctcttaaa tatattaatc
    15901 ttggaaatgt atttaactat ttatatactt ttaatcaact tggaagcagt actaatagga
    15961 cttctatgat aaaaaatagt ccaaattcta taattaataa ctcaaatgca ttaaacgatt
    16021 tttcagtatc cctaaaccaa gttaattttg atagtttaaa tattttatta ctaatatcat
    16081 ggataattgg aattttaatc atgatagcta ttactattca ttgcaacatt aaaataaaaa
    16141 atatgaaaaa atcaatacaa attttaagag atgaggagat agtatttata tttgaaaagt
    16201 gtaaaaaaga tattggtata aataaggatt ttatactatg caaatcacct attataaata
    16261 cacctattac atttggatac tttaaaccac acattatatt accagataag tctatatcta
    16321 aattatcttt aaaagatata aaatatatct tacttcatga attacaacac tttaaaaata
    16381 aagatatact gataaattat ataatgtgct tccttcaaat attatattgg tttaatcctc
    16441 ttgtatggta tgcatttaaa gaaatgcgta tagaccgtga aattgcatgt gatatttcag
    16501 ttttaaaaaa gcttgataaa gatagtcata tagaatatgg acaaactcta ataaactttg
    16561 tggataatat ttcaagacca tcaaatctta cacttacatc agatattggt gggtcaaaaa
    16621 aacaaataaa aaagcgacta ttgaagataa tggattttca taatgaatca aatacgttaa
    16681 aattcaaaag cattttaatt tttacattga tagctttaat tgtatttgga aatattccca
    16741 aactatctat tatggcagat atcaatgaaa aatatagacc tacaaatgaa caaactgtgt
    16801 acgaggactt aagtcagtat tttaaaggtt ttgatggaag ttttgttatg tatgatttaa
    16861 aatccaagca atatcatatt tacaataaag aaaaaagtaa aacaagaatt tctccaaatt
    16921 ctacttataa gatttatagt gccttattgg gattggaaaa tgggataata acaagaaata
    16981 attcacatat cccttgggat ggaaagcatc attctaatag ttcttggaat gaagaccaag
    17041 acttattttc tgctatggaa aactccgtaa actggtactt tcagactcta gataaaaaaa
    17101 cagggctaaa aaacatacaa acttatctta ggcaaattgg atatggcaat tatgatgttt
    17161 caggtggtat ctcaaacttt tggctagaat cttcattaaa aatatcacct attgaacagg
    17221 tagaattact tgaaagtttt tatacaaata agtttaaatt tgaagataaa aatatagaaa
    17281 cagtaaaaga ttcattgtta atatcaaaaa aggatggggt atcacttttt ggtaaaacag
    17341 gcactgccaa tataaatgga aaagatgtaa atggttggtt tataggttat gtggaaaaaa
    17401 atgaaaatac ttatttcttt gctacaaata ttcaaagcaa agattattct aatggtagtt
    17461 cagcatctaa gataacatta tccatattaa atgacaaaaa aatctataat ccttaatata
    17521 tttactgtac attctaaaaa attcacataa ttttcatatt aattatcaat taaattcata
    17581 tacaaggctt attatataaa catagaagat agtaaaacta gcttaaaaac ataatataat
    17641 accctataca aatagtggca actctccctc acatcctcaa ttgttgccac tatttaattt
    17701 tttgtatgaa gttaaatata ttttcatcat ttcaacaatt tgggtgcaat acaatctcta
    17761 ttttgaaaca acctctttaa ttgttttaga gtttatgaat tatttctcat actttaaaaa
    17821 gattgaatta ctcttgataa tcttcttttg tagtcattac atatcaccta ctactcaaca
    17881 catattcttt gacctatttt tacatccttg tttatatcca aaatatttac cttcttacca
    17941 tctggaaaca ataccattgt taaagtagac tttcctaatt catgtattct ctttaaatca
    18001 acattaacaa gaacatctcc ctgtttaacc atatctcctt gtttaacagc aatatcaaag
    18061 ccatctccat ttaatgcaac tgtatcaatt ccaacatgaa ttaatacttc tccattagtt
    18121 cctttaatcc caaatgcatg tccacttgaa accactgttt ctaatttacc tgaaatagga
    18181 gaaacaattt ttccatctat aatatcaata gcaaaaccat cacctaacat ttttttagaa
    18241 aatgtttcat ctggtacatt ttctaatgca acaattttcc ccttagctgg agacacaaag
    18301 ttttcattat tttcattaaa cactaattct tcgtcttcat cttcatttat agcttcttta
    18361 ggcactccaa ataaacatgt tacaataaag cagaatgcaa ccacaaatat tgatgctaat
    18421 aagtatgtga ttaaattata tccatcatag atatacatta aataagaagg taaaactgct
    18481 aatccatatg aattagattg aatatgtaaa attgataata ttgctgctcc taaacctgaa
    18541 gatgcacaca taataatcaa aggctttaaa ttatatcgaa gctgaatccc aaataaaaca
    18601 ggctcactta taccaaaaag ttgagatagc attgctccaa ctgcagtttg tttaacttga
    18661 ttatttttag attttttcat aaatgctaga catgttccaa tattcgcaaa tccatacatt
    18721 gcacaaagag taataagggc attaaatcct gtatttgcta atagtgatgt ttcaatcatt
    18781 acataagtat gatgtaagcc agtaataaca gctaatggat atgtaaatcc aataataaat
    18841 cctccaattc ctaaaggcaa atcaatcaat aagctgatga tatcaaccat ttttaattct
    18901 attgtatgca ttatagggcc tacacctaac aaaataacta acattgttat tagcataaca
    18961 aaaaatggtg tgaaaataag gtctaataca ttaggcatat gtttacggaa ccatttttca
    19021 agattagctc caataaatgc tgtaacaata gctgttaaaa cacttccttg acaacctaca
    19081 ataggaataa atccaaatgc cataattgct tcaatttcat ttgatgggtc agcaacagaa
    19141 tatgcattag gaagtattgg ggaaactaac ataagtccaa taacaagtcc aataacaggt
    19201 gttcctccaa atactttaaa tgctgaccat acaataattg ctggtaaaaa agcaaatgct
    19261 gtttctgtaa gaaccgaaac aagtgtctga atatacaatg gaatatttgc agaagacatt
    19321 ccaaataatc caagtacatt atcattaaac aaacaaccct ttaatcccaa gaataatcct
    19381 gttgctgcaa taactggaat aataggaaca aatatatctg ctagagttct cataactttc
    19441 ttaaatccag tttcattatt tttaactaat tcatcttgtt ctttttttga taaagttttt
    19501 aatcccattt tttcaacttc tgcataaact ttattaacta ttcctgttcc taaaataatt
    19561 tgatactgtc cagaagtaaa gaaaacccct ttaacctcat caactttttc aactttctta
    19621 tcattaataa tttctctatc tttaaccatc actcttaatc ttgtggcaca atgtgtaata
    19681 gaaataatat tatcttcacc aataatttta ataagctcat ttgctatttt attatattta
    19741 ttcatttttc tacctcactt tcaataatac tgcatgaaat ggtttcaaac gcactgtttt
    19801 atttaataga tttatatcat ctaaattttg gaatacaata tcatattttt cttctactct
    19861 tatttctaca ggtttatcag aaaaattata ataacaatca ataactacca aatctaattg
    19921 ccttctatac gcaataatat catcactttc tatatcaata ggttctattg aaccataaat
    19981 caaacaatca gaatactcac tatcttgtct caactttatc atctttttat aaaattctaa
    20041 aatactctct gaattgttaa tctgactctg tgcattaata tctttataat tttcaataac
    20101 ctttaaccat ggtgttgtat ctgaaaaacc tgcattttct ttatctgccc atggaaatgg
    20161 cgttcttgca ttatctcggc ttcttaaatt cacaaattct aacgcttctt cttctgagta
    20221 accttcatct attgaacgat agtattggtc aatgctagat aaatcattaa aatcatttat
    20281 ggtatctctt ttaaaatttg tcattcctaa ttcctgtccc tgataaataa atggggttcc
    20341 tcttaaaaag aaatacattg ctcccaacat ttttactgca tcatcattcc tttcgtattg
    20401 caaaagatac tttgttgtag ctcttggttg gtcatgattt tcaataaaat tagctgccca
    20461 tccatgttcc tgaacataca tttgactatc taaaatgcat tttcttaaat ctggaattgt
    20521 ccatgaaatt cttttaaacc actcactacc agaagcaaca tctaagtcag catgtttaaa
    20581 gtcaaagacc attgaaaaga aaccatcttc tccaataaaa tcacctaact ggtcatagct
    20641 aatacctgct gcttcagcaa cagtcataca atcatatttt ttaaacgtat tttcttttaa
    20701 ttcataaagc attttttcta ttccaggctg atttctaact gtttttgtac atttaacaag
    20761 accatcaaca ccatcacttt ctaaactttg aaaacttaaa ttctttttga tgaatgtaat
    20821 agcatcaact ctaaatcctg ccactcccat ttgagaccag gtattaatca tttggtaaag
    20881 ttcacttctt aatattggat tttcccaatt aaggtcaggt tgtttttttc caaaagaatg
    20941 aaaataatat tcgtctctat tctctacctt ttcccaaaca cttccaccaa aaacagacct
    21001 ccagttattt ggcaaatcgt ttcctttttt aaaaatataa taatcatgat aaggactttc
    21061 tggatttttt aatgcttctt gaaaccaagg atgttcatca cttgtatgat taagaactaa
    21121 atctaatata attttgattc ctctatcttt acttttttta attaactctt ttaaatcttc
    21181 catacttcca aattcacttg caatatccaa ataatcacta atatcatacc cattatcatc
    21241 cataggtgat ttaaaaattg gacataacca caatgatgta atacctaaat cttttaaata
    21301 atctaaacga gaaataattc cctgtaaatc tccaattcca tcattattac tatcttgaaa
    21361 gcttcttgga taaatttgat aaacgatttc tttttgccac catttctttt ccataaatta
    21421 ctccttttta tatgatattt cttcttttta attctttata aattccatta tcgaatatgt
    21481 cctcacatat agatgtcgct atatctttaa gttgttgatg actatttttc attgcaattg
    21541 taacgtctga tgcttgaaac atgacaatgt cattttgacc atcaccaaaa catatggttt
    21601 ctttttgagt tactcctaat ctttcttgta atctcttaat tgctttgccc ttatgaaaat
    21661 ctttttgaat aatttcataa tactgactag aaatatctct ctgtgctaat tccattttat
    21721 cttgtagtat atctttcact tcgtcaaata ccttttcatt tgaccataaa caaattttat
    21781 gtatatcttg tgacttatac tcttcaatgt tattttcata tgttattttt tcttggatat
    21841 gttgtttatt tatacaagaa ttagttcctt tcaactgatt cattgtttca aatatttctt
    21901 tggctttttg attcatgaaa actttttcct gactctcaat cgaaaaagca acttctctat
    21961 tttttaataa acaaacaact tcctttatca atctttgatt aaaagattga ttataaagta
    22021 attcaccatg atattgaatg taattacctc ctcctgcaat atatccatca actcctaatg
    22081 ataaaacatc atcttgtatt gtacccatac ttcttccagt acaaataaca acactgcaat
    22141 gattcttttg acataaacgt atagcatgct ttgcagattc tgggattcca tatacctcat
    22201 ctctaagcgt accatctata tcaagtatta ataatttgta caatttcatc ttcctttctt
    22261 actatcaaaa tcataatagc attcaaaaaa aaagatatca atattttgat atcttttaaa
    22321 aaatatgttc atttttagca caaattttca ttttcgggga atatgttcac tttctaaata
    22381 catcttgtat ttaatataaa gcagttcaat taaaataaaa taaggtgttg ttacatcgta
    22441 gtccagttgt ttatctacag aaatagatat agattgaata tacaggtttt catcacataa
    22501 tcttgctaaa ggattactta tcatttttgt aatagaaacc atataaattc ctttcattct
    22561 catttcttta gcaaaatcaa taattgtaga tgcatctcca ttcaatgaaa taaagaaaat
    22621 catatcatct gtttttataa aatgattaag tgcctgaatc atatcataac cataaacatc
    22681 atatataatt ttccctgttg gtaagaaaat ccttttcatc tctttagcaa ctctttcctg
    22741 agaataacct tctccaaaga cgattatacg atttgccttt ttcattcttt caaacataat
    22801 cgaacaatcc ttcttctcta tatcatttat caccttatta tagcaatcca ttaattggtg
    22861 tttactttga tttttatatt ctacttctgt ttcatttaag cgtataataa ctcttaattc
    22921 actatatcca ggtaattgta gtttttgagc aaaacgagaa agtgcacttg tagatatatg
    22981 gtatttgtct gcaaattcat ctatagaaag tttaatacaa tcttctttat gattaagaat
    23041 acactcacag atatacttat catttgctgt aaatgaatta taatatttat tgaaaagtcc
    23101 ttcaattttc attcttttct ccttctatac tctatgtaat tcatatgaag caaatctaat
    23161 agagcataaa atgctcctac gacttcataa gatatagctt gtaatgtatc aagtttttga
    23221 gtttcaacat acagattata ctcacaaagt gttgcaattg tattattatc aagcctagtt
    23281 aaagaaatcg tatgtatatg attgttgata aattttgcaa tttcaattcc tgcttttgtt
    23341 tctccagaca aagaaatgat aataaataaa tcactttttt gaaatgattc tctcataaat
    23401 tcaatttcac ctatatcaaa taagtcaata acacatttac ctactgaaag aaatattctc
    23461 tttatttctt gagctaaact tttctgttca ttacctgtcc cataaatata aattgtttct
    23521 gcatcatata ttattttaca aatatcttga aatgatattt tttgtaattc attcattaat
    23581 agtcgataat tctcgcaaac atcatgaaaa ttattatcat tattaacaaa ttctagttga
    23641 ttatttttaa gtaaatactt taaatcagag aatgaatcta atccaatttt tctacaaagc
    23701 cttaataaag tagctctaga aacattacat tcatttgcaa ttttttcaca cgaaaaatta
    23761 cggtattctc tttgacaaat caatatttgt ttaataataa ttttatcatt ttcatttaaa
    23821 ttatcataat tttggttaac taacgattct aatctcattt tttccactcc ctaaatatta
    23881 ttttaatata cattagttgc tttttctagg gaagagtttt aaataataag gataaaataa
    23941 tcatttgatt ttatttttat taaaataaaa ataacctgct aaaagcaggc accaaaaagt
    24001 acttatcctt tcgaacatgt actttagata ttgctttctt cggaagcttc atctcttcat
    24061 actctaaaat agagtttttt ctaactcaaa agtattttcc atggtagaaa ttactttaat
    24121 tcatctgcca tttatttttt atcttattta ataattacta ctacttgaat atttacttct
    24181 ttaagtaaat ttcatagtta acaaagctaa aattattatt atagatgtta aaatacaaaa
    24241 ttgtaagtat tgttataaat atgcttacag ctacaaatat acatgatata ttaaaaagaa
    24301 gctgtaaatt attatcatac atacaagcaa ctacgcctct attaatccat gttaattttt
    24361 attatttaat acaaggagtt ccatgagaca tacatatcca ttcatatttt aaattatcca
    24421 attctcttat agatttcatc atctttttat aatcccaatt ccatggatta ggataaggaa
    24481 ctatatttcc attcttattt ttgactaaat cacctgcaaa taatacatct tcaaaaatca
    24541 tgcagacatg ccctggagta tgaccaggag tatggataat ttttatattc ccaatctgca
    24601 tattctctcc ataaggttca acatccttaa tgagccttct ggaaattgcc tttccaataa
    24661 acttcttaaa cccaggtcta tccttctctc ctataataaa aggtatatcc tctacatgag
    24721 cccatacttt tgctcctgtt ttttctttca ataatttaat attagatata tggtcaacat
    24781 catgatgagt taataaaata tgcttaatat ctgtaagttt aatgccaagt gtttttaatt
    24841 ctttaattat atttttcccc ataaaaggaa gacctgtgtc tataagaata atttctttat
    24901 caaacacaat aaaacaattt gagccacgag tactatctaa cattaataca ttttctttta
    24961 tcttcataaa taaattcccc ttatttggta ttttacctat ataattaatt aataaagtta
    25021 taaatagtta taatacttga tattaatttt a
//
LOCUS       DAMJZE010000035        24669 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJZE010000035 DAMJZE010000000
VERSION     DAMJZE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.035"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     regulatory      95..185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            597..914
                     /gene="flgB"
                     /locus_tag="PC541_001348"
     CDS             597..914
                     /gene="flgB"
                     /locus_tag="PC541_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2838818.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            922..1326
                     /gene="flgC"
                     /locus_tag="PC541_001349"
     CDS             922..1326
                     /gene="flgC"
                     /locus_tag="PC541_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2838819.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1336..1653
                     /gene="fliE"
                     /locus_tag="PC541_001350"
     CDS             1336..1653
                     /gene="fliE"
                     /locus_tag="PC541_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2838820.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1678..3228
                     /gene="fliF"
                     /locus_tag="PC541_001351"
     CDS             1678..3228
                     /gene="fliF"
                     /locus_tag="PC541_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2838821.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3241..4311
                     /gene="fliG"
                     /locus_tag="PC541_001352"
     CDS             3241..4311
                     /gene="fliG"
                     /locus_tag="PC541_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2838822.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4304..5035
                     /locus_tag="PC541_001353"
     CDS             4304..5035
                     /locus_tag="PC541_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2838823.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5039..6358
                     /gene="fliI"
                     /locus_tag="PC541_001354"
     CDS             5039..6358
                     /gene="fliI"
                     /locus_tag="PC541_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2838824.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            6390..6836
                     /gene="fliJ"
                     /locus_tag="PC541_001355"
     CDS             6390..6836
                     /gene="fliJ"
                     /locus_tag="PC541_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2838825.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6864..8069
                     /locus_tag="PC541_001356"
     CDS             6864..8069
                     /locus_tag="PC541_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2838826.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            8087..8695
                     /locus_tag="PC541_001357"
     CDS             8087..8695
                     /locus_tag="PC541_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2838827.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8722..9705
                     /locus_tag="PC541_001358"
     CDS             8722..9705
                     /locus_tag="PC541_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2838828.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9718..9921
                     /locus_tag="PC541_001359"
     CDS             9718..9921
                     /locus_tag="PC541_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2838829.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9921..10739
                     /locus_tag="PC541_001360"
     CDS             9921..10739
                     /locus_tag="PC541_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2838830.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10729..11424
                     /locus_tag="PC541_001361"
     CDS             10729..11424
                     /locus_tag="PC541_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2838831.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11436..11879
                     /locus_tag="PC541_001362"
     CDS             11436..11879
                     /locus_tag="PC541_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2838832.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11894..12268
                     /locus_tag="PC541_001363"
     CDS             11894..12268
                     /locus_tag="PC541_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2838833.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12269..12934
                     /gene="fliP"
                     /locus_tag="PC541_001364"
     CDS             12269..12934
                     /gene="fliP"
                     /locus_tag="PC541_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2838834.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12947..13216
                     /gene="fliQ"
                     /locus_tag="PC541_001365"
     CDS             12947..13216
                     /gene="fliQ"
                     /locus_tag="PC541_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2838835.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            13228..15042
                     /locus_tag="PC541_001366"
     CDS             13228..15042
                     /locus_tag="PC541_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2838836.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15051..17126
                     /gene="flhA"
                     /locus_tag="PC541_001367"
     CDS             15051..17126
                     /gene="flhA"
                     /locus_tag="PC541_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2838837.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17101..18117
                     /gene="flhF"
                     /locus_tag="PC541_001368"
     CDS             17101..18117
                     /gene="flhF"
                     /locus_tag="PC541_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2838838.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18114..18992
                     /locus_tag="PC541_001369"
     CDS             18114..18992
                     /locus_tag="PC541_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2838839.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19006..19707
                     /locus_tag="PC541_001370"
     CDS             19006..19707
                     /locus_tag="PC541_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2838840.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19723..20145
                     /locus_tag="PC541_001371"
     CDS             19723..20145
                     /locus_tag="PC541_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2838841.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20130..20315
                     /locus_tag="PC541_001372"
     CDS             20130..20315
                     /locus_tag="PC541_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2838842.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20356..21129
                     /gene="flgG"
                     /locus_tag="PC541_001373"
     CDS             20356..21129
                     /gene="flgG"
                     /locus_tag="PC541_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2838843.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21150..21911
                     /gene="flgG"
                     /locus_tag="PC541_001374"
     CDS             21150..21911
                     /gene="flgG"
                     /locus_tag="PC541_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2838844.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21934..22815
                     /locus_tag="PC541_001375"
     CDS             21934..22815
                     /locus_tag="PC541_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2838845.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22818..23192
                     /locus_tag="PC541_001376"
     CDS             22818..23192
                     /locus_tag="PC541_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2838846.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23224..24627
                     /locus_tag="PC541_001377"
     CDS             23224..24627
                     /locus_tag="PC541_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2838847.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
BASE COUNT        10231 a         2637 c         4582 g         7219 t
ORIGIN      
        1 gtaattattg gtaattaaat ttaaaaaata aaatatttaa ttatacacaa aaaagatatt
       61 taaaaaattt gttatttatt gtatacttaa gttaaaacta aataggcaaa tctagagaaa
      121 tctagtgacg caaagctata gggactaagg tttatataca taaactatgt cagccagttg
      181 ccaaaaagag tcctaggtgt attgtatacc taagaaaagt ctataatgac tgcctttttg
      241 gcagtcattt tgttatggaa aaatcacttt atttcagtag aaaatacaca taataacata
      301 ttttaccaat tataacataa aataaagaaa aattatctca aaaagttact attttacaaa
      361 aaaaaatgac ttctttattt atttaaatta atcgcatttc cattacatta atacaataat
      421 gtatcaattt agtaagaaaa ctaatatttt tttaatgaag tcccatgaaa atataaaaaa
      481 attttaatat gtatttaaat atttcttcga aaagttgctc ttttttgtaa tatagcaact
      541 tcattttttt tattaataaa caaaatagat aaaatatatt ttgaggaggg gtgagtgtga
      601 atacatatga cattataaaa atgggattag atgcaactaa tctaagaagt cagacaatag
      661 caaataatat agcaaatata aatactgaag gatataaaag acattatata acttttgaag
      721 aaacactaaa tgatgctatg cctaaaatag aagaaaaaac agacaattct aaaggtttaa
      781 gagtagatgg taataatgta gacatagaag aagaaaaagt aaatcaagca atggcaacat
      841 tacaatataa tgctttgata agttttgcaa atggaaaaat agcaatgacc aaatcaataa
      901 taagtgggag gtaattagaa gatgagtgtg ttcagtggaa tgagaatatc tgctagtgga
      961 ttaacagcag aaagtttgaa aatggatatc atatcatcca atgtagctaa tataaatgct
     1021 acaagaggtc caaatggaga agcatatagg agaaaagttg tttcatttga agaaaactat
     1081 gataataaat taggtatgct aggagtaaaa gttacatctg ttgaagatga taaatcacct
     1141 cttaaagaag tatatgaccc atctcatcca gatgcaaagc caaatggata tgtagaatat
     1201 ccaaatgtaa atatattggt agaaatgaca gatttaataa cagcttcacg agcatatgat
     1261 tctaacgtag atacattgaa tgctcagaag tctatgatat caaaagcttt ggaaatagga
     1321 aagtagggat aaattatgcc aattacacaa attaacaatt taatgcaagg tgcaaagtta
     1381 gataatttga ttggagaagc aaagccatta actcttaata ataaagaaga aaaaaagaat
     1441 tttgaagatg taataataga tactatagat aaagttaatt ttcaacaagt aacagcagat
     1501 gatatgaaag aaaaatttat aaaaggcgaa gatgtttcta tgcatgaagt tatgttaata
     1561 ggtcaagaag cacaactatc tatgcaatat ttaatagaag ttagaaataa gattcatgat
     1621 gcatatcaag aaatgaataa ggtgcaaatt tagccttttg aacaggagta ataatcaatg
     1681 ataaccagtt ttaaaaataa gattaaggag caagttgcta aatctaggac aaagataagt
     1741 tctttaaaaa agggaactaa aatagcatta ggtatagggg ttattgctat atgtttagcg
     1801 ctagtattta cagcaatatc tgcgagcaaa aataagtatg cagtcttatt ttcagaacta
     1861 gatgctcaag atgctgcaga tatatcagca gagctagagt ctaaaaagat agatactaag
     1921 gtaaaaggaa gtacgattta tgtacctaga acagaagttg ataagttaag gctggagtta
     1981 gcgccaagtc ttacaggtgg ttctaaagga tttgcactaa tggatgatag ttcatcaata
     2041 gggcttactg atgaagaatt tcagattaag aaacaaagaa tgatacaagg tgaaatagaa
     2101 aaaacaataa agagttttcc acaagtggaa gacactagag tacatataac atttggagag
     2161 tcatcagtat ttgagaagga ggctgttcca gggagtgcag cagtttattt gatgctaaaa
     2221 ccagggacga ctttagaaaa aaaccaaata aagtctgtta tggcacttat atcaggaagc
     2281 gttacaaata taccagagaa aaatgttcag gttattgata acaaaatgaa tttattatct
     2341 gaaggtattt tggaggatga aaatgttgat gaaaataatc aaccaataaa tagtggcatt
     2401 agtgttgata aaaataaggc atctgaacaa gcattgaata aacaactaga aaagtctatt
     2461 ttagaaatgc tagaaccgat atttggtaag ggaaaggtga aagctacagt aaatgctgat
     2521 ttaagttttg atacagctga aaaaactgag ataaaaattg accctgataa agttgcaata
     2581 aaggagacaa ggtcgaaaaa ttcatctaaa tctgctgcaa ataatgttca aggtgtagat
     2641 gctaacatga ataaccaagg aaataacaat ggtactaata cagaagatag tttagatgag
     2701 agttttgagt atgaaacagg taagattgaa actcacacta tagttgcgcc tggagaactt
     2761 aatagattga cagcttctgt tgcaatagat ggaaaaatag ctaaaggtgt tcaagctgat
     2821 gtagaggata ttgttaataa tgctataggt atggatggag cgaggggaga tactcttagc
     2881 gttgtttcaa tggtatttga cccagaaggt aagaaagagg ctaaacaaga gctggaagag
     2941 attaaaaaag aaggatttaa aaagaacata ataaaagcag ttattggagt agtagcagta
     3001 atagcagttg gagctattgg atacttaata tataagaaaa gaagtgaacg agaagatatg
     3061 gattatgaag atgaagatta tatttctaaa tctgattctg ttgataggaa ggataatcct
     3121 ctattaggag atgacccaga actgacatta gaggaagctg ttaaaatgta tgcagaagaa
     3181 aagccagacc aagtaacaga gataataaag acttggttaa atgagtaagg gggatagaaa
     3241 gtgtccacgg aaattacaaa tgttaatgcg tttgacttaa gtgatataaa tgaaagtgat
     3301 aagaatgaga aggtagaagg tgagggttta aaaaaagctg ctgtattatt aatgacatta
     3361 ggcccaaatg tgtctagtga tataataaaa agcttgcctg ataaacaagt tcaaaaaata
     3421 ggtttggaaa ttgcaaatat ttcttcaata gcttcaaaag aaagaagaga aatcttaaaa
     3481 gagtttgtag aaataaatag ggcaaaagat tatgttatag aaggtgggct tgaatatgca
     3541 agagagttat taagtaatgc tttaggaaat gttaaagcaa acaaattact tgaaggtata
     3601 tcaataaata caagtacaaa gccttttaaa catattataa aactagatgt aaagaagata
     3661 ttagaaatac ttaggaatga aagtgcacaa actatagccg ttttacttgc acatatggaa
     3721 ccagaaaatg gagcagaagt tttatcatac ttagattttg atttacaaaa tgaagtagct
     3781 atgagaataa gctcaatggg ttcaatatct ccagaagtaa taaaggtaat tgaaagttca
     3841 ttagaagata aattacattc aataagtggt gatgatttta atgattatgg aggggtgtct
     3901 accttagttc aaatattgat taattctgat agaaaaacag aaaagaatat tttgggaact
     3961 attgaagaac aaaacccgga tttagcagct caaattaaat ctcatatatt tgtctttgaa
     4021 gatattgtca agttagatga tataagcata caaaaagtgt taaaagaagt gactatgaaa
     4081 gatttagtat atgccttaaa aggtgtagat gatgagattt cagatgctat atatagaaat
     4141 caatcttcta gagcagcaga tgcacttaaa gaagaaatgg aaatggttgg tgttataaag
     4201 gttacacaag ttgaggcttc tcaacaaaag atagctaata tcgtaagaaa acttgaaaga
     4261 gaaggtgcca taactattgt aaggaatttt ggtggtgact ttgttgaata ataagattat
     4321 taaaagtaaa aaagcattat ataaaggagt gctagattta accataatag ataaagttag
     4381 agtgggagaa tcaaatgtaa ataaagaaaa tgattgtaga tttagggatg aggtaataaa
     4441 agaagctata gaatctgctg agataagaaa gaatgagata ttagaagaag caaaaatgaa
     4501 tgcacaaaat atagaaaagc aagcatatga aaaagggtat agtcaaggtc aaaaaaatgg
     4561 atatgaagat ggatataaag agtcatatga tgaatatacc caaaaagcta aagatgaagc
     4621 agattttata aaaaatcagg cagacttaat actattacaa gctaatgaga agatgactga
     4681 gtacataaaa gaaaaaaata aagagattgt aagacttgct attaatatgg ctagttctgt
     4741 tctaaaaaag catttcgaag atgaagaatc tatgagtgat ttgttaaagc aaattattca
     4801 agattatgaa ggaagtagtt catttatagt aagatgtaat tctttctata aagataatat
     4861 agagaaagag tttgaattat tcaaagagga aacatcacta aaatcaagtg tatttgttat
     4921 tgcagatgat agtatagata aaggcaatgc tattatccaa aaggaaaatg gaagaataat
     4981 agttggtatt gattgtgcta tggaaaaact aaaagaagaa ttgcttggtg aataaagtat
     5041 gtataaaact gatatatcta tagattttga caaaataaat aagaaaatag aaaatttaga
     5101 actcactata tcagaaggta gagtaaagaa gataatagga cttactgtag aagttgaagg
     5161 aataaaagct tttgttgggg agttatgcgt tatatacaac caagttaata aaccagtgaa
     5221 ttgtgaagta gtaggtttta aagataaaga agtcatatta atggcgttgg gagaattaac
     5281 attgatagca cctggttgta aagttatttc taaggggata cctttaagtg ttatgtgtag
     5341 tgataattta ctaggtaaag tacttgatgg tcttggaaat cctatagaca actcagatgc
     5401 tgttttagga gatagataca acctgaacaa tgaacctcct gacccaatga agagaaaaaa
     5461 gatacgaaat ataatggaaa caggtgtaag agcaatagat gcatttacta cttgtggtga
     5521 aggacagaga atcggcatat ttgcaggaag tggagtaggt aagagtacga ctctaggtat
     5581 gattgctagg aatgcaaaag cagatgtaaa tgtaattgct cttattggag aaagaggaag
     5641 agaagtacta gattttattg ataaagattt aggtgaagag ggaatgaaaa aatctgttgt
     5701 tgtgtgtgca acatcagata aagcccctct agttaggtta aaaggtgctc ttactgctac
     5761 tgcaatagca gaatatttta gagaccaagg caaaaaagta atacttatga tggattcagt
     5821 aactagattt gctatggcac aaagagaagt tggtgtagca ataggagagc caccagctca
     5881 aaaaggatat acaccatctg tatttgcaat gttgccaaaa cttatggaga gaacagggac
     5941 atcagataaa ggttctataa ctgcatttta tacagtatta gttgatggag atgactttaa
     6001 tgagccaata gcagatacaa caagagggat acttgatggt catatagttt tatctagaga
     6061 cttggcaaat aagaatcact atccttctat agatgtatta aacagtttaa gtaggcttat
     6121 gaatgaaatt gcttcaaaag aagacataaa aatagcgtct tttgcaagag acatgcttgc
     6181 tgagtataga gaagcagaag atttgataaa tataggtgca tatgcttcag gtacaaacaa
     6241 aaaaatagat gaagcaatct attatcatga gcatattatt aattttttaa aacaaggaat
     6301 aaatgaaaag agttctttta atgaaactat aagtagttta agaagaatct ttgaataaac
     6361 aatttaaaaa aatatttgaa taggttgtga tggaattgga taaaaatttt aagtttagat
     6421 tacagaaagt tttagactta aaaataaaag atgaagaaga gataaaaatg gaatttgcta
     6481 agattcaaca aaaaaaaata gatatagaaa ctaatttaga aaacttagaa agtaattaca
     6541 gcaaatattc tataagtaaa aataatgata atgttcaaaa tcaaaaaata actattaact
     6601 atttgctagc tttaaacaac tctataatgg atttatcaga agaactggat aagtcgacaa
     6661 atgaacttga aaaagctaga aaacagctta taagcaaaca gatagagaga aagtcattag
     6721 aaaaattaaa agaaaaaaaa tatggacagt attataaaga agaacagtta aaagaacaaa
     6781 gcactaatga tgagtttgca agcatgagtt atttgagaaa tagacaagtt ctataaaata
     6841 ataggaagtg aggatattat tgtatgaaga tacaagcaga ttcttataat attattaaag
     6901 atattgggaa tagaaaaaac tcatctaaga ataaaaatat aaaaaataat aaatttgaag
     6961 ataatctata taagacaaca gaccataaaa atgttaaaga tagaagagaa acaagtaagc
     7021 aaaatgttag ttcagataga ccgggaaata gtgataatga gtgtcattta gatgaagata
     7081 aaagtaatca cttagaagat gaaaatacat atgaaaaaga ttgtagtaag aataatgaag
     7141 ctttacagca attactgaat cttttaaaag agaattataa aaaagataaa gatataaaca
     7201 ctatattgaa taatataaaa aaactaagta tatcagatga attaaaacaa gaacttaagc
     7261 ttaataaaaa tatattgaat aaaattgcaa atcaaaattt agagttaaat ggagctaata
     7321 caaataaccc taatgatata gaaaatttag acataatgaa gttaagagaa cttttagaaa
     7381 ataatgctag tgaagatatt caagtaatta gtaaaactac agaaaatgat actaaaagca
     7441 tagacttata cagttttgat agtaatagaa tggatactct taactcaaat aaacagaaag
     7501 atgatagttc tgatattttg gaaaaactag cagggttaaa tggaaataag agtggaaatt
     7561 ttggacaagt aataaataaa agcgttgata tgaataaaat taaaaatgat aatgttaatt
     7621 taaaacagat agaatctaat gtaatggatg attctataaa agctattaag catatgaaaa
     7681 caaatgatat acaagaattg acaattaaat taagaccaaa agaattagga gatatgaaca
     7741 tacaattatt aaaagatggt gaaagtatga gagctgttgt gactgttttc aataaagatg
     7801 tctttgactc tataaataaa aacatagcag atttaaagca acatcttgaa cttacaaatg
     7861 taaatataaa agatgtatca gtgcaaatgc atagcgataa tacaaataca tctgatacct
     7921 ttgataaagc atttgaacaa cagaatagac aaaataacca agaaaacatg agtaatcaaa
     7981 atacaaatga taagcgtaac aatgtagtca tagaagatga gattaaagaa aatattattg
     8041 atgatgatag agtagattta ctagcttagt aagggggaag tattgcatgt acggaatagc
     8101 aacggctttg gcaggaactc aggtaaacta taggtcaaat aacttaagcc aacttgaaag
     8161 aaatattaat gatattgcca acaaacaagt tagaagtaaa ggaaatcaga cagtaacaga
     8221 caatgggact ccaattatag gacaaggtga tgatgctgat aaagacttgt ttatgaagat
     8281 tcttgtagca cagatgggta atcaagaccc aatgaatcca caagacccaa cggagtatgt
     8341 atcacaatta gcacagtttg caactttaga aaaaatgtca gctatgaatg ataagctaga
     8401 tatactttta gccttaagta actctactct aataaactct gctttgagta tggcaacatc
     8461 attaattggt aaagaagtag aagtatctgg ggaagaagat gaagatgaaa acattgtagg
     8521 agtagtaaag tccacataca taaaagatgg ggaagtacac ttagaattag aagtagatgg
     8581 aagtgacaaa actgtagatg ttaaatatga aaaattactt aaaataggag aggtagaggc
     8641 aaattcagga gaagaaaatg actcttcaga acaagaatct ccagaagaag tataatatta
     8701 aatgggaaaa gaggtaaatt tatgataaaa gcaatgtatt caggtgtaag tggaatgaaa
     8761 gcaaatcaaa caaaactaga tgtaatcggt aacaatgtag ccaacgctgg aactacttct
     8821 tttaagaaaa gttcagctag aataacagat tcattttatc aaactgtact atatgcaagt
     8881 gctccaacag cggcattagg gggaacaaac ttaggacaag taggtgtagg tagtaagata
     8941 tcaagtataa acaaagatat gacacaagga aatgtacaac caactaatag accatctgat
     9001 ttaatgatag atggagatgg atatcttcca gtagtaagac aaggaacagt tatgtatacg
     9061 agagatggtt cttttaattt agatacagga tttaaagcag ctgatggtgc tttgaagaat
     9121 attaaaggtg gaagacttgt aaatggtgat ggatatatgt tacaaggtat gatttatgat
     9181 ggggaatata aagctgatac tggagaattt gttaaaaagg gtgatgctaa aggaaaagag
     9241 cctattgtaa taccacttca aagtataagt cctaaggatg gaacagttca aaatgtacta
     9301 gagtataata tatcaaagga tggaacagta gaatttttat tgagtgatgg tcaaagaact
     9361 caatttgtct atgatatacc tgatggtgct aaggtaggag atacacctgc tggaatagat
     9421 aaattaaaag gtacaataca aaaagtgcaa acatatgcat tccaaaatcc agcaggacta
     9481 gatgcagaag gaggaaactt gttcaagcca tcagcaaact caggagaccc acaaatagca
     9541 ggagctacag gcacaataat acaaggagct atagaagctt ccaatgtaga tatagcagaa
     9601 gaatttacag aaatgataat agcaagtagg tctttccaag caaacagtaa aataatatcg
     9661 acatcagacg aaatacttca agaaataata aatcttaaga gatagtaggt acatattatg
     9721 ataagagtaa cggatttaaa cggaagaaaa tacatattaa attcagatct tatagtgaga
     9781 attgactccg tgccagaaag caggattctc cttacaacag gagaaaaaaa tcttgtaaaa
     9841 gagtcagtag atgagatagt agataaagta ataaagtata aaagaaaaat aaatgtaggt
     9901 tatattagga gtgaaaaata atgaaaaaaa cagattcact aacattcatt ggactaatat
     9961 tatctacagt attagtttta tttggagcag taaaaggaag tagttcagga ttgaaaagtt
    10021 ttttcgattt tgcatctata ttaatcacag tatttgggtc atttggagct ttgatgatta
    10081 catttactat tgacgatata aaacttataa aagatgctct acaatactca ttcaagacaa
    10141 tgaatatatc gaaattagac ttattaaatc aatttaaaac actttctaaa aaagcaagaa
    10201 gagaaggact tttatctata gaaagtgatg taatgaaaat acaagaccca tttatgaaaa
    10261 aaggattaga gttatgtatt gatggtcttg aaataaatga aataaggtct atactagagc
    10321 tcgaaataga ttctgttgaa gaaaccaata acagatattc taaactattt aaaacttggg
    10381 ggactttcgc acctgcattt ggtatgttgg gaactctaat aggtttaata caaatgttat
    10441 ccgaccttac atctccagat ttaatagcat ctggtatggg aaaagcatta attacgacat
    10501 tttatgggtc tttacttgcc aatatcgctt tgaacccaat tgcatataat atagatgaga
    10561 agactgaaaa agaaatttat gtaaaggaaa tgatgctgga ggggataata agcatacaat
    10621 ctggagagag cagcatagta gttgaggaac gtttagctac atacctttca aataatgaga
    10681 agttagaaat tatgaaatcc aataaaaata cagaaagagc aatgagtaat ggcgcgtaaa
    10741 aaaaataaga aaaaggatga gataaatcca gatgcatggt tggctactta tgcagatacg
    10801 ataacactga ttcttacatt ttttgtgtta ttgtatgcta catcagttgt agatatggaa
    10861 aaatttaaaa atatagcaga tgcgttaaaa ggtcagtttt ctgggacaag tatgtttagt
    10921 acaggttcaa gtacaagtga gaaatctcca atagagagtg ttttagtgga taaaaatgat
    10981 aagtcaaatt tagaacagga tttaaaagat aaagttaata attctaactt acaaaatagt
    11041 gtaacagtca aagatgatag tagagggatt ttactagagc tagatgattc aattttattt
    11101 gactctggga tttctgaatt aaaagaagat agtaaagcca ctcttaataa agtatacgat
    11161 atgataaaga tgatgaaaaa taagatagtt atagaaggcc atactgataa tgtacctgta
    11221 aaatcaagta cgcatgaatc caattgggat ttatcttcta gtagagcagt aagtgttgta
    11281 agatactttg tagaagaaaa gggcatggac cctaaactat tttcggcaac aggatgtgga
    11341 gagtaccaac ctttggtatc aaatgaaaca gcaaagggaa gagctgagaa tagaagagtt
    11401 aatatactta taactattga ctaggaggaa gaggtttgtt caaaaataaa aaaattatta
    11461 taattatttt gatactgatt attttctcag caggagcagg tttttttata ttaggagaag
    11521 gtggaaataa acaaattgac aagattgcag gtttgtttat tccaacaggg aaaggcaaag
    11581 atgaagtaaa gaccgaaaat cttgacttag aagagatggt attgaagcta gctgatgaag
    11641 gtgtaagata tttaaaagta tcaataactg tatcttatga ttcttcatat aaagatatag
    11701 aaaaaaatac gcctttaatt agggataata ttataaattg ttttatggca aaaagggcag
    11761 atgattttac tgctgataat ttaaagaaca taaaagaaga aattaaaaat acattaaata
    11821 tacaactagg tgaaaatgta attcaaaata tatatttcac aaatatagta gtgcaataaa
    11881 aggatggtaa gacatgatac tttatttact aaaaatatta atatgtatcc caataatttt
    11941 aatattgata gtcttatctt taaaaattag ccaaggaact ttattaaaaa aatataatga
    12001 taaatatata aaaatactag atattgtaaa tataagtaaa aacaatagca taataatttt
    12061 aaaaataggt gaagaaggat gcattatggc atcttcttct tctagcatga gtaaaataaa
    12121 agatttgacc aaagaagaaa tattaagaat agaaaacgac attaaagaaa gaaattttaa
    12181 aattcaagat tttaaaatgg aaagaattaa aaagaaatat gatttcagta gatatattgg
    12241 aaaatttaca aaaaaggaag ataagtaaat ggaactatta tcaaaattac ctttggctga
    12301 tgtaccatat acaagttcaa tgcaattgtt tttatttcta actgcattaa tgtttttacc
    12361 atttgtaatg ttcatgatga caagctttgt cagaatagtt ataagtttat catttttgaa
    12421 atcagcatta ggagcacagc aagcaattcc aagtcaaatt ttggtagggc ttgcaatagt
    12481 attgacaata tttataatga gaccagtatt aaatgagata aacgaaaagg cattacaacc
    12541 atatatgaaa gaagaagtta ctatggaaga agctatgaag gaagcagaag gacctattaa
    12601 agaattttta ttaaagcaaa ccagacagac agatttagat ttatttgtag agcaagctgg
    12661 tttaaaagaa aagaaattaa ctcgtgaaaa tataccatta tctgttgtag ttccagcatt
    12721 tgctataagt gaactaaaaa ctgcttttca aataggtttt ttaatatata taccattttt
    12781 gataatagac cttgttgtgg caagtgtttt gatgtctatg ggtatgttta tgttacctcc
    12841 agttatgata tcattaccat ttaaattatt attatttgta atggtggatg gatggaactt
    12901 gatagtaaaa acattgatat tgggatttgg ataggagaaa aatagtatga gtgaatttat
    12961 gataatgagt gttcttaaaa atggcatgtt tacaggagct atgattgcag ctccagtgct
    13021 aattgtttct cttgtagtgg ggcttttaat aagtgtattt caagccacaa ctcaaatcca
    13081 agagcaaact ttgacttttg taccaaaact aatagcaatt cttttagtag tagctattgg
    13141 tggaagctgg atgttacata ctcttattag ctttacttcg gagatattca atatgatgtc
    13201 tctcataact aaataagtag gatatatatg atttttgtat ttattagaat agtagcattc
    13261 tttggaacat taaggatact atttccagct ggtacacctg ctttatttaa gtcgctcttt
    13321 gcaatcatta tatcaatctt gatttcaagt actatgcaaa ttgaatatgc aacaaatata
    13381 gacaacattg tattgtttac cttatatggt ataaatgaga ccataactgg gattatgctt
    13441 ggatatataa ccaatctatg tttttatagt atcagaatgg ctggttctat gatggaccaa
    13501 cagatgggat tatcaatgat taatatgttt gacccaaatt caatgactca gacaacttta
    13561 atcaataatc ttatgaactg gacagcactt atgatatttt ttagcatgga tggtcatcat
    13621 gttttaatta gaggcataag gtatagtttt gagttaattc caatagggaa atcattcata
    13681 gataataata tagattatat aataaacata tttgtgcagt gctttttaac tggattcaaa
    13741 atagcaatac ctattgtact ttgtctgctt atggcagatt ttatattagg gcttatatca
    13801 agaagtgtac ctcaactaaa tgtaatgata attggtatgc cattaaaaat actggttggt
    13861 attgcattat ttatcatatc aataccttta attgtcaatc aaataagtca tttattaagt
    13921 caaataccaa agatgtatga gggaactttt gcactagctc ctatgttttt tatgggttca
    13981 acagataaaa cagaagaagc tactccaaag aaaaagggag aacaaagaaa aaaaggtaat
    14041 atagcaaaga gtaaagaatt acccattgca atgactctac ttgcattcac tttattagta
    14101 cctacattat tttcatatgt agtgaataca cttaaatctt ctcttaatta ttttttgagt
    14161 ctagattttt atatgaacat aaattattca agtatagaga aattagttat agcaggatta
    14221 atggattttt ttaagatatt tttaccaata gctattccat tcttagtttt aggtatagtc
    14281 gcaaacctat ttcaagtagg aatactgttt actggtgaaa ctttaaaacc caatttatca
    14341 aaattaaatc caattagtgg attcaaaaac atgttttcta tgagaagttt gtcaacctta
    14401 ataaaagata cagcgattat atctattttg gcttacatag gatatacatt cttccaagat
    14461 aattatttag atatattaaa gttaggtaac atatacctgc caactttaat gtacacagtt
    14521 aaagatttag tatattcaat attaagtaag atttgtgtag ctatgatagt tatagcagtt
    14581 gcagattatg tttatcaaag atacagtcat aaaaagcaac ttagaatgac aaaacaagaa
    14641 gttaaagatg aatataaaaa ctcagaagga gacccagaaa taaaagctaa gataaaacaa
    14701 aaacaaagac aaatttcgtc tcaaagaacc atgcaagcag taccaagtgc tactgtaata
    14761 gtaaccaatc ccacccactt atctatagca gtgagatatg aaaaagggaa agaccaagct
    14821 ccagtagtgg tggcaaaagg tgcagattat ttagctttta aaataaggga aattgcaaag
    14881 ggaaatgaca tacctataat agaaaataaa cctattgcta ggttgttata taagcaagta
    14941 gaaatagacc aagaaattcc agaggacatg tatcaagctt ttgcagagat attagtagca
    15001 gtatataaaa tcaaaaatag atacaaagtc cccaaaaggt aggataagta atgaataagt
    15061 ttaatttaaa ggaaaatatg gacgtaatag tggcatttgg tgttgtaggg attgttctta
    15121 tgatgatagt gccgttacct acatttatat tagatatttt actggcaatt aacataagtt
    15181 tatctgtgct aattttacta atgacactat ttacaacaaa tgtacttgat ttatctatat
    15241 ttccaacagt gttacttgtg acaacactat ttagattggg attaaatctt tcatcaacta
    15301 gattgatttt aacacaggga tatgcaggtg aagttataga ggcatttgga agttttgttg
    15361 taggtggaaa ttacgtagta ggaataatta tattcttaat tatattaatt gttcaatttg
    15421 ttgttataac caatggttct ggaagagtat ctgaagtaac agctagattt acgttagatg
    15481 ccatgccagg taagcagatg agtatagatg cagatttaaa tgcaggagtt atagatgaca
    15541 aaactgctag aaaaagaaga aaagagcttc agcaagaagc agatttttat ggctctatgg
    15601 atggagcaaa taagtttgta aagggagatg ccatagcagg tcttatagta acagcaataa
    15661 acataatagg tggaattgta ataggagctg taatgctgaa tatgactctt atgggtgcag
    15721 cacagacata tgtaagactt actatagggg atggtcttgt aagccagata cctgctctat
    15781 taatatctac atcagcaggt ataatcgtaa caagagcttc tactgatgaa aactttggtg
    15841 gattagtagg taaacagtta acagcaatac caaaagcagt aatgatgact ggctgtgttc
    15901 taatcgtact tggtatgatg cctggattac ctaaaccagc atttttttca ctaggtatag
    15961 gtgctatagc agcaggttac tttttaggaa gagaaaagaa agatacttta gaagacttgg
    16021 agtttgaaaa acctgatatg gtaaatccag ataaaaatga agtagaagat gtaacaaact
    16081 taataaatgt agaatcaata gaagtagaaa ttggatatgg tttaatatct ttagctgatg
    16141 aatcagcagg aggagattta cttcagagaa tagcatcaat aagaagacaa tgtgcaatag
    16201 acatgggtat agtcgtacaa cctataagaa taagagataa tttacagtta aatccaaacc
    16261 aatacaatat aaaaataaaa gggaatgtag ttggtgggta tgagataatg cctaatatgg
    16321 ttttatgtat ggatcctatg aatcaaggat ttagtatatt tggaataaag acaattgagc
    16381 ctacttttgg aatagaggca ctttggatag aaagtgacaa ggtagaagat gcagagctaa
    16441 agggttatac agttgtagat tcatccacaa tacttataac tcacttacta gatataataa
    16501 aatcaaatgc acacgaatta ctgggaagac aagaagttaa gactattata gatgcagcaa
    16561 gagaaaatta tagtgctgta gtagatgaat tgataccaga tttaatgaca cttggtgaaa
    16621 ttcaaaaagt acttaaaaat ctgttaagag aaaaagtaaa tataaaggat agagttacaa
    16681 tacttgagac acttgcagac cagtctagaa atacaaaaga tatagagctt cttactgaat
    16741 atgtaagaat agctttggct agaagtattt gtaataattt agttgatgaa gataaggcaa
    16801 ttgtagtggc tacattgtct ccagaaactg aagaattggt atctaataat ttacaaaggt
    16861 ctgtaaatgg aacttatcca gcagttgacc cagaaaatac aaataaaata tttgagagta
    16921 tacaagaagt aatgaacaat gtatatttca atcataatat accagtaata gtagtatcac
    16981 caaagataag agctccattt agaaaattag ttgaaattgt atatcctaat ttgactgtat
    17041 tatcattaaa tgaaatacct aacgatatta aaatcaaagc agaaggtgtg gtgagcatat
    17101 atgatgacga aaaagtatac agctaacaca attcaagatg ctatgaatct agccaaaatg
    17161 gaattgggtg acaatattac attaatagat aaaaaagagg taagaaaatc tggaataaga
    17221 ggcatttttt caaagaaaga tattgaattg actattggat gggaaaagaa agataattta
    17281 gaacaaaaag atttaaaacg agagatagaa caactaaagt caattataaa taatatgggg
    17341 tttgataata aaaatgataa tgatatagat aaaatatgca agaatttact aaacttagat
    17401 ttaaatcaag aaattgtaga atttataaaa gctgatttac aagaaatgaa gttcaatgga
    17461 attgatacaa gtaaaaattt agtagaaagt ctaaagaaaa agattaaaat agaaaatcaa
    17521 gcaataaatg gtaaaattgc tttagttgga ccaccaggag taggtaaaac gactacaata
    17581 gcaaagttgg ctgccaaatt agtttttgag gaaaataaaa aagtaggagt aataactata
    17641 gatacataca ggattggtgc tgtagaacag ttaaagatat atactgatat aatgaatatt
    17701 ccttttaaag gggtaataag ccctgatgag atggaattgg cacttgatga gatgaaagat
    17761 tgtgatgttg tattaataga tacaacaggt agaggctata aaaactctat gcaaatatta
    17821 gaaatcaaaa atcttataga taaagctgaa actgataata tacatttagt tttgaactgt
    17881 acaacaagag aaagtgatac aaaagcaatt atagattcat atagaaatgt gaattttaaa
    17941 agcttaataa ttacaaagtt agatgaaaca ataacatatg gctcaatatt taacataatg
    18001 aattatgcac aaaaaccaat atcatatatt acaacaggac aaaatgttcc tgatgatatt
    18061 ataaaaccta atgaagataa gattataaga ttactattag gagttgaaag tatatgatag
    18121 accaagctca aattttgaga aaaatggcaa tggaaaaaag aggttctgat gagattatag
    18181 tagagaatga gaatatgcct aagataatta caatagcttc tggtaaaggc ggcgttggaa
    18241 agagtaatct cgctacaaat ttgtctatat gcttaaccaa acttgataaa aaagttctta
    18301 tattagatgc agatatagga atgtctaata tagatataat aatgggggta aatgtaaaag
    18361 ggactataat tgatgtaatt aatggcgaaa aaaatataga agatataatt tcacagacta
    18421 agtatggtgt aaatataata tctggaggtt ctgcccttaa tcatatagaa gactttacag
    18481 aagctcaaag aaataagttt atacatagca tagaacagat tcacaatgtg gattttataa
    18541 taattgatac aggagctggt atgagcaaaa gcctattgtc atttatatac tgtagtactg
    18601 aatttttctt aataacaact ccagaaccga catcattgac tgacgcatat agtttattaa
    18661 aagctataag taattttggc ataaagaaaa gtgcaaatat aataatcaat agggtaattg
    18721 atttagaaga agcgaaatct acatacaaaa gaataaaaat ggttgtggac aaatttctaa
    18781 accttaatct ggaattgtat ggatatataa tagatgacaa aaaagtaggt gtatgtgtaa
    18841 aaaaacagat accattcatt ttagaatatc ctacaagtat tgcatcaaaa tgtattatag
    18901 ctatagcaaa gagaatggta agtcagtcaa gagaagatat tgttgataat aaaagttttc
    18961 taagaaagat gtttagtata tttaaaagct aaggaggcgt agttaatgaa tagagaagaa
    19021 ttaataaaag agaatatgcc tcttgtaaag agtatagcaa gaaaattttt tataccaggg
    19081 aaaggttttg aatatcaaga cttagtaaat agtggagtca ttggattgat tgatgcaata
    19141 aataaatttg atactgaaaa aggagcaaag ttttctagtt actcttatat aaagataaaa
    19201 tctgctatat tagatgaaat tagaaatcaa agtccaattt ctaaacataa tctaactaaa
    19261 gtaaataagt ataatagagt ggtagaaaag ttgcaatcta aattattaag agagccaact
    19321 tcatatgaaa ttgcaaaaga attaaaggtt tcagaaaaag aacttcatga tatcgaaagt
    19381 aatatagata tgttaaatat tgtatcatta aactatgtaa tctttgaaga tacaaatgaa
    19441 actgtacaag atgtaataag tgatagagaa gaggaagctc cagaaaatat aatagaagaa
    19501 gaagaaaagt tagagattct atccaaagca attagtaatt taaatgaaag agaaaaatta
    19561 atactttcac tgtactatta tgaagattta aatctaaagg agatagggaa agtgctaggt
    19621 gtttcagaat caagagtttc acaactccac agaaaatcaa taagaaactt aagaaacaag
    19681 ataaaagaac ttaaatattc tatatagatg gtgattgata agatgataca aattatatta
    19741 atagtagtgt ctattttaat aatagtaact atagttataa atttacagaa ggaatcaaaa
    19801 aataaagata acagatactt tgataaaata tttgagcaag aatatgaagc aaatgataga
    19861 ttaataattg cagaaaaaag aagattcttt ttagacaaag tacttgcaga aaaaagaatt
    19921 aatagtcata taaaagttta tgaaaattat aacctaataa aggctaatga agaaagaaag
    19981 gaaaacatat ttatagaatc aaaaaaggaa gtaagacaag aaaatgagtt atctcttaaa
    20041 gtgaaacatc ttataagttc aggttatgat gatgtggaaa tatgtaagat gctagatatt
    20101 ggaaaggggg aactgttatt aataaggagt tgttacaaaa tataaggata ttattatgtg
    20161 ataaaaaagt attcttagga ataggtattg gtatattaat atcaacttta tgtataatgc
    20221 tttttagtaa taatgatatg agtaaggcag agatagaaaa aaaggccaga gcgttaggca
    20281 tgaaatatac aagtgagatg aaagttttag aatagatgat actgtttaga catttacaaa
    20341 aaaggaggtc ataatgtgat aagaggatta tatacagcag tttctgctat gataactaat
    20401 caacaaaagc aaaatgttgt tgtcaataac ctagctaata tggatacaaa tggatataaa
    20461 agtaaacaac ttttgactaa aagttttgat gaattaaagt tggagggtta caataactac
    20521 gcaaatggaa ctaaacaaaa gcagattata ggcgatataa gtccaggtat ttctatggat
    20581 gaaactatca caaattttaa tcaaggacct ataaaaacta ctgataataa aatggatgta
    20641 gctattcaag gtaagggatt ttttctagtt agtgatgcag ctggaaatca attttacact
    20701 agaaatggga acttcagaac agacaatcaa ggtgacctta taacaagtga agggtattat
    20761 gtacttggga cgaatactgc tactggtgct gtaggtcata taaatgtagg aaatcaaaaa
    20821 tttgaagtgg caaaagataa tacaatatca ctagacggaa atgctatgta taaatttaat
    20881 attgtagatt ttaataacta caataactta aaaaaagaag gagataactt atattctgga
    20941 ggaggagcta tgaaagtaaa taatgcactt acaatacaaa gtgcgataga gtcctctaat
    21001 gtagatatgg tatctgaaat taataatatg atgacaatat caagagaata tgaggcaaat
    21061 caaaagataa ttcaggctat ggattctaaa ttagcaaaaa tagcaagtga gattggttca
    21121 gtaagatagg taataggagg tgaattttta tgtacacaat gttttatact agtaaaacag
    21181 ctttatcagc aaatcaaagt aaactagata taatatctaa caatatagca aattctgatt
    21241 caacaggata taaaaaaata aatatggaat attctgattt agtggatgaa gtactaaata
    21301 gaccatcata ccctacaaat ggtaaagata tatcgactgg aacaggagct aaggcaacag
    21361 ctcccatgag aaattatgaa caaggtgcat tggctcctac agatagtaag agtaatttgg
    21421 cgatagatgg agaaggattc tttagagtga taagaaaaga tggtacatat gcatatacaa
    21481 gaaatggtgg atttaatgta gatgctttag gtaagattgt agatgataat ggaaatatat
    21541 tagatgtcca atttgatgca ggatataact ataataatac aaatatagat agtacaaacc
    21601 tgacagtttc aagaaatgga gagctattcg tagataataa aaaaatagga aaaattaact
    21661 tatatcaacc tataggaact caaaatttta tatctgaagg agatagttta tttgtcgcaa
    21721 ctgatgcagc acagattaaa caagttgaga aagtcgatat agttcaaggc tatagagaga
    21781 ggtcaaatgt ttctttacaa gaagaatttg ttgacttaat agccactcaa agagcatttc
    21841 aaatgaactc aaagggaata aagacagcag atgaaatgtg gcaaatagcg aataatttaa
    21901 gagctaaata attatgtaag gagaggaata ttcatgaatg atatgaaaga gtacgatttt
    21961 ggcaaacctc agagttattc aagagaaaat ttaacttgtt taaatttttt attagctgaa
    22021 ttttgtaaaa aatataaaaa ctatataatt tatgaactta agtgtaattc tagtatggtt
    22081 gttgatagta tagaccaagt aaattatcag acttttttag atagagtaac ttcttcatgc
    22141 gtagtagtac aaaatgcaat ggaacctgtt atgaaaaact tttcttttag aatagataga
    22201 agtgtagctg atatgtggat tgatataact tctggaggaa ctggagttgt aaaggataat
    22261 gatagagagc ttacagaact tgataaaaaa gtgcttttac accttatgga tgatttagta
    22321 agaaatatgc acttatttga gggttttgaa aatatacaag tattggatac acatacacat
    22381 attaatttgc cacaattatg ttctccaact gcaccagtat gcgttgtaga tttaaaagtg
    22441 ctaaatgata atgagtgtat tggaagtgtt tctctttgtt ttccatacaa tggtttagag
    22501 gctctgttag aaagtatatc tgttatagaa ttttttgata atgatgtagg agatgattca
    22561 gaggaattta cccaaaaaat ctataatagt gtatctaata tagaattatc agttattgca
    22621 gagataggaa aagtatcaat taatgttgag gatttactta aactagaagt tggagatgta
    22681 atagtaacaa ataaaaaaat aaatgattat atagatatat ttgtagaaaa ttcaaaatca
    22741 tacacagcaa caccaggatt tatatcaagt aaaaaaggtg taaaaataaa tgatgctgtt
    22801 ggaaaagagg tgtagttatg gacaatagta atcttatttc agatgaagaa gcaaagatgt
    22861 tattaaatga taaagttgaa agtgaagttg atggagatga tgagttagcc ttagatgtct
    22921 ctgaagttaa tcgaaataat atacaaagga tacttgacct taagttagaa ttatctgtag
    22981 ttataggaag aactaagatg actctaagag atattttaaa tttacataaa ggttctctta
    23041 ttgaactgga tactttagca gaccaagatg tggaaatact aatagataat aaggttttgg
    23101 cttatggaaa agtagtggtg gtagatttaa attttggtgt aaagataaca agtatagtaa
    23161 gtgaagaaga catggtaaaa tctcttgttt agagataaca tcaattaata cttaaggcat
    23221 gaggtggtac ttattttaaa cttaaatgat ataaaaaaag atatactaaa actcaataaa
    23281 aaagactcat gttctctata taatgaatct attaataata taaataataa ttgtatatca
    23341 actggattaa aatttttaaa acaagcaaga gatttaaacc cagatgatgt tgatatatta
    23401 aatctcacag gtcttgtaaa cttactaaaa tgtaattttg atgatgctgt agaaagtttc
    23461 tataagagtt cactttgtga aaaaactgct ctgtgtgaaa agtatataaa catgcttaca
    23521 tctgaggagt ttttaatttt ctttgaaaag tacaatcaac ttataaggtt aataaataaa
    23581 ggaatgtata aagaagctat acaagggttt aaactcataa ctgaccaata ctgtgattta
    23641 atagaacctt atgagttgct tgccttactg tatgacaagg aagaagaata tataaaattt
    23701 gatgaatgtt tagaagtatt gaagattatt gataaggaaa attatttatt aaatcaagaa
    23761 aataacacat tagatatgta taatagtgct aatatacaaa ctaaaagtaa tataaaagta
    23821 gatgattata agaatgagtt cttacataaa agtagtagta accataaaat taatgattat
    23881 aatgatgtaa ctaaagaaaa gagttcaagc aaaaaccaga aaagttcaga ttctaataag
    23941 aaaaaaagta ttaaaaaaaa taagtaccta tatatagcca taatattagg aatattatta
    24001 gtaggacaaa gtatatataa ttcttataag atgaataatt taactgataa aatatctaaa
    24061 aataatgaaa ctaaaaatga agagtcaata gattctaact tgactaagaa agaaaataat
    24121 atagaagaaa atgaagatat gagtaaggct tctaatataa ataagagtaa taaaaacatt
    24181 aataaagagg atgatgtaaa atctaaaaaa aatactgtag aaaatgaaga tgataaagat
    24241 tctgacttat ttacagagga tgaattgatg tctaaggcga atactttaaa atcagaaaag
    24301 aaaactaaat cttctataca actatataag aaagttgcag atataggtga aaataaaggg
    24361 aatacatctg aagcaactta tcaagttgct attctcagtg aaaagttaaa agactataaa
    24421 actgctgaag aatattataa aatgtatgta gaaaattact cagaaaaaga tgcttatttt
    24481 gatgaatctt attataattt aggtatgatg tactataaca atggtgattt aaaaaattct
    24541 aaattgacat taaagaagtt ggttaataag gtaccaaata gtatgtataa taattcaaaa
    24601 gtaaaagaaa ttttaaaaga agaatagtta aattagataa gaaaaataaa ttattttatt
    24661 ttaaaataa
//
LOCUS       DAMJZE010000036        24514 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJZE010000036 DAMJZE010000000
VERSION     DAMJZE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.036"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(433..609)
                     /locus_tag="PC541_001378"
     CDS             complement(433..609)
                     /locus_tag="PC541_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838848.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            1184..2206
                     /locus_tag="PC541_001379"
     CDS             1184..2206
                     /locus_tag="PC541_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838849.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            2220..3107
                     /locus_tag="PC541_001380"
     CDS             2220..3107
                     /locus_tag="PC541_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838850.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            3113..3886
                     /locus_tag="PC541_001381"
     CDS             3113..3886
                     /locus_tag="PC541_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2838851.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            3998..4723
                     /locus_tag="PC541_001382"
     CDS             3998..4723
                     /locus_tag="PC541_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838852.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            4727..6130
                     /locus_tag="PC541_001383"
     CDS             4727..6130
                     /locus_tag="PC541_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838853.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            6141..6965
                     /locus_tag="PC541_001384"
     CDS             6141..6965
                     /locus_tag="PC541_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2838854.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7290..7910)
                     /locus_tag="PC541_001385"
     CDS             complement(7290..7910)
                     /locus_tag="PC541_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2838855.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            8176..8508
                     /locus_tag="PC541_001386"
     CDS             8176..8508
                     /locus_tag="PC541_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838856.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(8628..9269)
                     /gene="pcp"
                     /locus_tag="PC541_001387"
     CDS             complement(8628..9269)
                     /gene="pcp"
                     /locus_tag="PC541_001387"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2838857.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(9290..10222)
                     /locus_tag="PC541_001388"
     CDS             complement(9290..10222)
                     /locus_tag="PC541_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2838858.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(10244..10909)
                     /locus_tag="PC541_001389"
     CDS             complement(10244..10909)
                     /locus_tag="PC541_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2838859.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(11481..11957)
                     /locus_tag="PC541_001390"
     CDS             complement(11481..11957)
                     /locus_tag="PC541_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HDF2838860.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(11960..12364)
                     /locus_tag="PC541_001391"
     CDS             complement(11960..12364)
                     /locus_tag="PC541_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2838861.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(12339..12968)
                     /locus_tag="PC541_001392"
     CDS             complement(12339..12968)
                     /locus_tag="PC541_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2838862.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      13334..13377
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13478..14446
                     /locus_tag="PC541_001393"
     CDS             13478..14446
                     /locus_tag="PC541_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2838863.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            14448..15011
                     /locus_tag="PC541_001394"
     CDS             14448..15011
                     /locus_tag="PC541_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2838864.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            15019..15708
                     /locus_tag="PC541_001395"
     CDS             15019..15708
                     /locus_tag="PC541_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2838865.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15835..16563
                     /locus_tag="PC541_001396"
     CDS             15835..16563
                     /locus_tag="PC541_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2838866.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            16577..17251
                     /locus_tag="PC541_001397"
     CDS             16577..17251
                     /locus_tag="PC541_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2838867.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            17277..18113
                     /locus_tag="PC541_001398"
     CDS             17277..18113
                     /locus_tag="PC541_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838868.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGKY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            18129..18800
                     /locus_tag="PC541_001399"
     CDS             18129..18800
                     /locus_tag="PC541_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838869.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            18790..20022
                     /locus_tag="PC541_001400"
     CDS             18790..20022
                     /locus_tag="PC541_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838870.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            20172..20489
                     /gene="trxA"
                     /locus_tag="PC541_001401"
     CDS             20172..20489
                     /gene="trxA"
                     /locus_tag="PC541_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2838871.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            20506..21384
                     /locus_tag="PC541_001402"
     CDS             20506..21384
                     /locus_tag="PC541_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2838872.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            21654..22022
                     /locus_tag="PC541_001403"
     CDS             21654..22022
                     /locus_tag="PC541_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2838873.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            22025..22396
                     /locus_tag="PC541_001404"
     CDS             22025..22396
                     /locus_tag="PC541_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2838874.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(22541..22996)
                     /locus_tag="PC541_001405"
     CDS             complement(22541..22996)
                     /locus_tag="PC541_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838875.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(23035..24300)
                     /locus_tag="PC541_001406"
     CDS             complement(23035..24300)
                     /locus_tag="PC541_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2838876.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         9461 a         2723 c         3789 g         8541 t
ORIGIN      
        1 tattatttat ataaatattg aaatttgtct agttatacgt ttttattgac cagttatgca
       61 gttgatattg aatattctaa atattggtgt tattttatag gtggtttaaa catattaggg
      121 ggattttata tatgatttgt atgatttata gagaatgtat aaaatagtaa aggtttagag
      181 ccatgtttag tggattgcag aatatgcata tagaatgatt cttgtaaatt aagttagaca
      241 taggaaatat ataatttgta ttggatgatt aattttatgg tgtatgcttt taacttctat
      301 aatgggaaag taaagatgta ctcccaatac atctttactt tagcagaagt tatagttact
      361 tataaagtac aaaaaattat atatttatta gagtagataa atctaaaaag gtttttctag
      421 aaaaatttag ttttaattgt atctacttac taataataag acttgatgta agcaggtttc
      481 aactaccgtt tctccatatt ctgatataaa atcatctaac tgtgtatcta aaaaattaca
      541 ataatcatat gaactactga tgtaagcagt tagtatatgt gtacatttgt ctatttctcc
      601 aataaacata ttatccatcc ccctaaaaaa tttagtaaaa ataaaaagtc gcattctatt
      661 agtcaattaa cataatttat aaaatttttt agtactagtt tctataactt aactccttaa
      721 ggttaaatat tcccagtttt accaatttag ttttattcta caagtatata atactagaag
      781 atgctccatt attcaacatg cttgtttgag aatagaaaat attctaaaat tataattgtt
      841 tttaaaaata agattcaaga taaataaagt tatatgcaac tatttaataa tctttccata
      901 ataaggacag taggttctat tcttaaaaaa ctccatcttt aaatttaaag aataactaca
      961 atttgaacaa caataaaatt tccaatcaat aaaaatatca aaaataataa aaataattga
     1021 aaatgtataa tttcccagaa aaataataaa tgtaactcca ataataaaca cacctataaa
     1081 ttataaaata atttaagctt tatttaaggt tagtattata tttatataag atttaagttt
     1141 taatatatat gtgtaattaa aatgatttag gaggagaaat atattggtaa attgggaaat
     1201 aaaaaaattg gctaaatcta agagtatgtt tatatctcta gggatattag tgttaatact
     1261 aatgattagt atctttatta cgcctgtttt agaaacagaa agttattata tagataaaga
     1321 aaaaggacgt atagaagata cgagaagtgg tatcgacgta ggtaatgaga aatttcaaaa
     1381 taagattaat gttttaaagc agttttcaaa tcaaacagat acaggtgaat tttctaaaaa
     1441 aattgaatta atgtcaaaga aaaaacttga taatctaagt gtgaatgaat ataaggatat
     1501 tagtttttgg aaagtattta actatagagt cacaaattca cttataaatg taggaatgtt
     1561 aatcattata tctataatta tatccaatat ttatgtagat gaagtcgtta cttcggtaaa
     1621 agatatcata ctatcatcaa aagagaagaa aaaagcatta aagtctaaaa tatttgttgc
     1681 tttattagtt ccattagtaa tttattctgt ttatttaatg ggaatattta ttataacttg
     1741 tatgcaacaa ggagtacctg ttaatggaga cttagaatct tttagaatag tagacaatgt
     1801 agttgcattg aaaactaatt taagtataac aaagtatgta atattaaata taggcataga
     1861 gcttttgatg tttgaaggat gggccatgat agccatattt gggtctttta taagtaagtc
     1921 ttcaatatct tcaataagtt tttttatatt tattatatct ataactaaga taatgtcggt
     1981 aatccatata ttacctaaaa aattactttc agttgtatca aatgtaaatt attatgattt
     2041 gatatttggt tttaataaaa taataggaaa ttatctaggt gatattatga tatttaaagt
     2101 gaatatagac attgtcaata tagcaatagg acttttgatg gcaatattga taggtgctat
     2161 gtcattatgt tttttagtaa taaaatcaga ttatataagt agataaaagg ggagttaaga
     2221 tgaataaatt agaaatagaa aatctttcaa agatatatgg taaaaaagta gcaaacgaca
     2281 agattacagt aactctggaa aatggagttt atggtcttct aggaccaaat ggagcaggaa
     2341 aatctacatt aatgaaacaa ataacaacgt tgataaaacc tgatgaaggt cagatacttt
     2401 ataatggtga ggatatattt aaaatggatg ctgattatag aaatatttta ggatatttgc
     2461 cacaagaatt tggagtttat aaaaatttta cagcaaaaga atttttacag tatattggag
     2521 ctttaaaagg tttaagggga aaatatttaa atagtaagat aggagaatta ttagatttag
     2581 taggtcttta tgatgtgaga aataagtcta taggaaaatt ttcaggaggt atgaagagga
     2641 gagttgggat agcacaagtt ttactaaacg accctaagat aatagtcctt gatgagccaa
     2701 cagcaggact tgacccacaa gaaagagcta gatttaggaa tttaatagca aaaatgtcta
     2761 gagataagat aataatactt tctactcata taatttccga tatagaatca gttgcaaagg
     2821 aaacaataat gattaaagat ggcaaactcc taatgaaagg aacacacaaa gacatattaa
     2881 gtcacatgga aaataaagta tataatgttc aagttaaaaa tgaatgtgaa gttgatgata
     2941 tacaatctaa atataaggtt gtaagtattc aaagtgatat tgattcagta gtacttagaa
     3001 ttgtttcaga ggatattcca agcgaggata atatacaacc agttcaagca aaattcgaag
     3061 atgtatacat gttttatttt gatttagaag atgcaaggga ggtgtagtag aaatgatgta
     3121 tttagttaaa gctgaaatga taaaaatatt aaaatcaaag agtgtttgga taatttggct
     3181 gtttttaata ttttttggat tttttctgat tagaaagttt gatgttttag atacttatgc
     3241 agatatattt tacaagatag aaggctcaat acctctaata ggtttgataa tgtttataat
     3301 aacttctgga aattacatta aagaatatga ttctaatatg acaggtttaa taaatacaac
     3361 taaaaaggga aaaaaatcag tagtattatc aaaactagta gctaatggat taattttatc
     3421 tattattaat atttctttta tattgttagt aggaattaga ggttttagtt tttttgattt
     3481 taaaaattta aatgaatcga ttcataactt atggtacttt ggaaatagta atttaaatct
     3541 tacagtaata caaatgtata taattgttat attgactact atttttgctt cttttatatt
     3601 tgcacaaata ggcttaacac tttcatctat atttaaatcg gctgtaattc catttatact
     3661 aggtggattg ataatggcaa ttccatactt tagtgtaggt tttataccag ataaagctat
     3721 aaaatttatg tcagtgactc caaattggat tatgatgagc caacaaatgg ttaagtataa
     3781 tgtaccaagt attttgatag ttttttctat agtaatctca attatattaa tgatagtgtt
     3841 aactaaaata acatatgaaa attttacatc aagcaaaagg ttttaactaa aaataatagt
     3901 aattttattt atattttagt ttaattaaaa ataagataaa tgaattttag ataaaatata
     3961 tgctaatata tatgtaaatt attacaggag gttgtttatg gaaaatcgtg ttttaataat
     4021 agatgatgaa gtagaaatat tgaagctttt agaaactgtc ctaaaaaaag agggccttaa
     4081 taatatatat actgcaaaaa ctaaaaaaga aggattagaa ttatttaaaa gtattaatcc
     4141 tgatttgatt gtattggata ttatgttacc agatggcgaa ggttatgata tttgtaaaga
     4201 aataagaaaa acctccaatt ctccaataat atttttatca gctaaaaccg aagaattaga
     4261 taagttactt ggactagcga ttggaggaga tgattatgtt acaaaacctt ttagccctaa
     4321 ggaagtggct tttagagtta aagcgcattt gagaagatta agttatttta gtgatgctca
     4381 aaacgaaagt aaaaatctaa ataataatga agaaaaaatt atatcatttg gcccatatat
     4441 attaaatgaa agtagggcag aacttataaa agatggaaaa tctataggac tgtctgcaaa
     4501 ggaactaaaa atcctatctt tatttgcaca taatcaaaat caaattataa gtaaagaaaa
     4561 actatgggat aaagtgtggg gagaagatta tgttggattt gacaatacta taatggttca
     4621 tataagaaaa attagggaaa aattagagga taatccttca aagcctgaat atattctgac
     4681 aataaaaggt cttggatata aattagcagt aaaggaagat tagaatatga agtggaagat
     4741 tactagaaat tttatattta caattgtgtt tgtagctata tcagtagtaa ttataaatat
     4801 catatctata ttgtatgtga tttcaactaa tagttttttt aaagttgtag attctggaaa
     4861 taatcctgaa gaatttgcac gttcttttga aaaagattta tatgaaaaag atggagaatt
     4921 taaactatct aagattggag ctgaaaaatt agaaaaatca aattcctgga tacaggtttt
     4981 aaatgattta ggagaggaag tttatggagt aaatgtgcca aaggatactc ccaaaaaata
     5041 tacacccttt caaatggtga ataattacaa gtatatagaa acaaagtatg ttaattttgt
     5101 attagaaaaa tatttaaaca aaaaacatct aaacataata gtaggtatac ctagtagaga
     5161 tatatcaaga ataattttaa cctattctca aaataacatt aaaaaaactt taaataaagt
     5221 tatcattata acactagtaa ttgatagcgt ggtagcttta ggtgtaggat atttatttag
     5281 tagaaaactt acaaaaccaa tttcaagcgt actatggagt attgagacta tggctaatgg
     5341 aaattatagt ttatatctta aggacagagg tatatatgaa gaagttttta aaaatataaa
     5401 tatgttagct gatactttaa gagtaaatga agttgagaga aaagaaaatg aagagttgag
     5461 agaggaatgg ttggcaaata taacacatga tataaagaca cctctagcat ctatacaagg
     5521 gtatgcagag ataattaatg acaaagatta tgagtttgaa gaagatgaaa tacaagagta
     5581 tacagaaatt atatataata agtctaagta tataaaagat ttagttgatg atttaaattt
     5641 atctactcga ttaaaaaata atactatagt tctggacaag aaaaaaataa atttagtcag
     5701 tttagtcaga gatataataa ttgatatttt aaatgataat agatataaaa ataggaatat
     5761 tgaatttgaa agtaatgaag atttaattga agtatatgta gattcaatct tattcagaag
     5821 ggctataacc aatcttatat ttaattcaat agttcataat agcgaaggaa ctttgataag
     5881 tgtagaaatt gtaaaaaaag ataatataga aattataata aaagatgatg gaattggaat
     5941 aagcaaaagt gatttgaaac atatttttaa aaaatattat agaggtacta atacaggtga
     6001 gatgcataaa ggttcaggtc ttggtatggc tatttctaaa gaaataatag agattcataa
     6061 aggtaaaatt tatgtaagta gtgaaattgg aataggaaca aaaattatta tagagataaa
     6121 acaaaattag gagatatgat atggattttt ttaaaaaagg tataagtggg tttactatta
     6181 agattttagc attaatattt atgacatttg accatatagc agcatttatg ccacaaacaa
     6241 tgcaaatacc aatttggttt cattgggtag gcagaatatc agcaccactg tttatattca
     6301 tggctgttga aggattttat cacactagta acagaaaaaa atatataagt agattgtata
     6361 tttggtctgt tattatggct ataggaaatc aaataattaa taatgtattt tcacatccag
     6421 aaggagctat aattataaat aacacttttt caacattatt tttaatagcg atttatttac
     6481 aagcaataga gttcataaag aaatttagaa aagagaaaga aattaaatat tttattatag
     6541 gattattgat gataatcatt cctataattt taggaatatt tacagtagct ttacttttta
     6601 aagtcactaa cagagttatt gctttattta tgattttggt accagtacca tttttagtag
     6661 aaggtggacc aatttggatt atacttggga ttatttttta tttgtgtaga ggtaaaaagt
     6721 tcagtttgag tatttgttat gttttaatgt gtatatttat atttacaaca atgtcaaatg
     6781 gagattatag tttaaaaaat tctattttac aaaattacca atggatgatg attgcttctc
     6841 tacctttaat gttactttac aatggagaaa aagggaagag tatgaagtat ttattttatt
     6901 tatactatcc aatccatgta tatatattgt atatcttagg aatatactta ataaatggtt
     6961 tttgattgta agtgatattt ttaatacaaa atgaacagta tgaggattgt tttaggtcta
     7021 ttaaaattgt tatttagatg agatttgtag aatatttaaa tttatgagca gaaattcagt
     7081 tattgtaatt tcaataacat tagaatataa tattataatt tatttagaaa tatatgatgt
     7141 tatacatggt ttggttatta atgtaataat aaaacagagg tttgatataa aattcaatac
     7201 ctctgtttta gtgcttttta tcctacattt atgcttattt tatttataaa ttagtgtttt
     7261 gttttaatta atattagtac attttttatt tattttataa aattagtcat aattgtttct
     7321 tcatattgtg gttctttaat tccagctttt ttacctgctg atatacactt aagaagccat
     7381 gccatgtttt ctcctagagt tcgcatagtc tgtaatcctt cttcatcctt cataacatcc
     7441 tctggagtaa agccatgtac ctggttccaa tactgtgatg atacaatagg catattgctg
     7501 attgaaaaat atttatttag ttggtcaaat gttgcacttg caccaccacg tctacatgac
     7561 acaactgaag cacctaattt tgtagccatg cgactcccag ctatgaaaaa tagacggtca
     7621 agaaaagaag ttaattgacc agttgcacta gaaaagtaaa caggagaacc aataataata
     7681 ccatcaatat tatccagatc ttcaatcaat tcattaactt tatcatttcg aatacatttt
     7741 ccagtttcaa aacaggatgc gcaactaata catcctggga ttgtttcttt acctaaatac
     7801 agtatttctg ttttaatatc gtgtttactc aatactttag taatctcatt tagtgctgta
     7861 taagtacatc catattggtt tgggctaccg tttatcaata aaactttcat tataaatttc
     7921 ctccaatgaa ttttatttaa tagattttct aagagacatt attttttata aaataggatt
     7981 ttcatgaata ttatcaattt atatagaaat gtctaatctt agaatcctat ttaaaataac
     8041 ttttttttta gtaaatacac ctcctattat aaaattgact ttcaattatt cttacactat
     8101 aattttatta taaattattc taaaaacaag tacgcacatt tttgttcaat actaaccaaa
     8161 aggagagtgt aatatatgag tatagactgt atatcaaata cagatttaag tgaaacagga
     8221 tttagttata cactttcact gattagtggt aaatataaga tgataattct ttactgtctt
     8281 gtagaatttg aagttgtaag atataatgca ttaaaacgtt atataaatac tatctcctat
     8341 aaaactttga gtttatcatt aaaagaatta gaagcagata acttgattat tcgtactgaa
     8401 tatcctcaaa tacctcctaa ggtagaatat agtctatcag aaaggggaaa atctctgatt
     8461 ccaattttag atgcaatgtg tgagtgggga gagaggaata gaccttaatt ttgtatctgt
     8521 agataccata aaaagactgt gagtaaaatt gtttgaataa tctactcaca gtctatattt
     8581 aaattaatag ttagtatttt taatttataa aaatactaaa aaatttatta tgaaatttca
     8641 ccaccaattt cttttacatc ttctttatat ataatacaag cttctatagc taattctaag
     8701 ccttttacta tttcttctaa agacatgaat ggtgtatttt ttttatctat tacttgtgaa
     8761 gttgtatatg ggatgtgtat aaatccaccc cttatattag gatatttctt gtctatcata
     8821 taaagaattc catacatcac atgattgcaa acaaatgttc ctgcagtgtt tgaaacacta
     8881 gctggaattt tattttcatt catatgtttt accatagctt ttacaggcaa ttttgaaaaa
     8941 tatgcaggtt caccatcttc ttttataata gtatctataa cctggttacc ctcattatct
     9001 tttattctaa aatcatctat atttatagca actctctcag gtgtaatgtc aaatctacct
     9061 ccagcttgtc caactgatat tactatatct ggattgtgct cttgaatagc tttatctata
     9121 gcttcaacag attttgtcat aactgttggt atagttatct ttatgatttc tgcaccgttt
     9181 atattattat tgaccctttc aactgcttct tgagctgggt ttattggttc tccgccaaat
     9241 gggtcaaatc ctgttaacaa aattttcatt attttacctc ctaattatat taaaatccta
     9301 aaaagtacat tactaatatg tgggctataa ttaataccaa agcaactgga gcttgatatt
     9361 ttataacaat ataatcattt ttacattcta gcaaggctgc tggaactata ttaaaattag
     9421 cagccattgg agtaagtaga gttccacaat atcctgctgt taatgctaat gctccaatta
     9481 tagcaggatt tccaccttga gacaatacaa aaggaatacc aattcctgcc gttatgactg
     9541 aaaatgcggc aaatgcattt cccattatca tggtaaatat aaccatacct aatacataag
     9601 ctataacacc aaataatctg ctatcagcag ggactacacc agatatcatg gttgaaataa
     9661 cttcaccgac tccggcttga gtaaataaag ctcccaatgc aactaaaagt tgtggtaaca
     9721 tactagcagg tcccatttgt tgaagtaatc ttgagccatc ttgaactgat gtagcaggtt
     9781 ttgccttagt tattataagg gctgatatta atgcaattat tgaacccaca cctattgcaa
     9841 actgagaaga attatcaaaa ttagaaagac tcatagctac tactactgca gcaaatgcta
     9901 atattaatga tggtataaat attttatttt taagtttgtc tgcttgttct tttccaaaat
     9961 tttcatctgg ttcttcatac tttgcaaata caacttgttt agaaagtgtt aataaactag
    10021 ataatattaa tattattcct attatattgg ctggaatcaa tctaccaaat atgaatggca
    10081 gtgatataag tatccaaaat aatgatgtac catatttctt attgttattt atggtagtaa
    10141 aagacttata tgctataaca atcattaaaa atcccattag tgcgtaaaag atttccaatg
    10201 atatgtttat aaaattttgc atttttattc ctcctaaata aaactatttt ttatgacttt
    10261 tgtttaattt tttatcatat aaggtaaact ggattgtagc cactacaagt gctattactg
    10321 ccataatagt actagccttt acgatattaa gtccagttac attgtatcca aattgagtca
    10381 gagtagatgc tataagtaat actccactac tacctgcaaa taagttttga ccataaaaat
    10441 ttccatagtt ttctgatgca gcagataatg cctttatggc ttcttcatcc gattctttta
    10501 gttcatcact attacttaaa ccagcagctt gtgccatagg attaacaagt ggtcttacaa
    10561 attgaggatg tccactcatt cttattgata gagcaccagc tatttgtcta gctatcatgt
    10621 atatagtaag tacttttcct gttgtaagtc tttttaattt ttctattaaa gaaacagctc
    10681 tttgtcttaa accatatctt tctaatacac cgattactgg caatgttaat atgaataatg
    10741 atacacctct gtttgagaca aaagaatctc ctaaaattgt aaaaatttga ttaaaatcta
    10801 atcctgcaac aattcctgtt gccactcctg ctaataatac agtaaatagt gtgtcaattt
    10861 taagtataaa tccaattaca actatcaata taccgattag ttttatcata aattgttctc
    10921 cttccatttc tatatgtttt aatttaaatt tatttaatgg ataaagttaa actcttttta
    10981 tagtatttgc gttaatagga ctattattat aaaatttaat aaaaaatagg gaagatgttt
    11041 taaagtatca aagtaaataa tggttgtgta taggaatttg aataacaaag attatatttt
    11101 aagctaatat gaattgattt tgtaagtcta tagtttaagt atatgtcaat atttgaaata
    11161 gtagttttaa attaaacatt atattataaa aagagaatgt gccaaacata gagacaagtg
    11221 tatttttctc tgttgaaatg ataaaaatat atttgatttc atacaaaaag agagtgtcta
    11281 atgaattttt tagttcatta gacactctct ttttgttatg attaataaaa catatttgtt
    11341 atgattaata aagcatatat taagtgctag tttcattaga tttcactaag aaaaataaaa
    11401 ttttgataaa gtttaaagac tattttgagc aatattactt ataaataaaa gaatttatca
    11461 tagaaataat tagctttatt ttagctatta agatttatta aaaaattatc tatattatcc
    11521 tgtgatattc taaaactgtc ataattacct tttgaaacag ccttcataat aagtttatct
    11581 aaaaatttca ttttttctaa tcgagcttca ctaccaaaaa ttccaatagc atttgcactt
    11641 tctaataagg atgatggaaa atttgatttt aaatatttat ctgcttgttc agaaaagcca
    11701 cagcaaagga aaatgccaac ttgttttttg tttaataact ctacattatc attacatagc
    11761 acctgtattt tttttgaaac tgaaccaaca tatatagaac ttccaataat aattttatca
    11821 aattttgata actcaatttt attattatta ttattaatat caacaatagt gacattatct
    11881 gatagtttat tttttagtat atttgcacag tcttttgtac aaccatattt acttgaatat
    11941 ataataatag tattcataat tattctccct ttcttaaagc attttctaca gcttttcgta
    12001 tagtattact gctataagtt gcagaagtta tagcatccac atctaaagac tgttgtttaa
    12061 taatacttgt tgtaattttt tcagcaggtt tacctaaaag atggtcgtgt tttaagatat
    12121 taatattttg gattttacca ttattaactt caacagacac agtagctttt accaagttat
    12181 tatcagcact tccagtataa atgccatttt taacattatt aatatctata tcttttacta
    12241 ccaagttttc tggctttaag ttgcttacaa ttacaataat aatgcatata aatacaaaga
    12301 atagagcaat agatattttt acatattttt tctttttatt agtagccttg ttgcgaattt
    12361 tcattgtaaa gtcctcccac taaaatatct aagaaatttt ctataagttg agaaaaatta
    12421 tgtaaagaat atagtttatt ttcaacaaca cataaaacga atcctaacaa tgcacttata
    12481 agcatccatg agcttttatc tatatttttt ttgaatattc cttgttcaat tccaatggaa
    12541 aggaatttat caagcatttt tataccatta ttgctaggcg tattgtttgt attttcagat
    12601 gcaaatataa tattcattct actatgcatg atagattttg ccatctctgg ttcactagaa
    12661 agagttttta taaagctagt cataatttct atgatttgtt tatctattgg tagtgatgag
    12721 caattattca taatgtcaat ggcattactt tctactgtgt tataaagttt attggtcata
    12781 tcgctgataa tctctgcttt gtctttgtag taaagataaa ttgtagttgg agaatattct
    12841 atttttgtag caatttttct tattgataaa ttttcatatc catgttggtt tataatttcg
    12901 atgctagcat ccataatctt tttcttcatt tcttcttttt catttttacg tctttctttg
    12961 atactcatat aatcacctct ttgcttaacg gtgttaagtt aatttacact gttattgtat
    13021 aatttaattt taatactgtc aataaattat taaataatat aagttaatat atttttatgg
    13081 ataataaatt aatgttatta attgtaatat ttaagatatt attgtataat aataatcggt
    13141 ttaaatgcgc cacatcttat taaagttggt tcataattct aaataaactt taataagata
    13201 cattaaaata taataaattc ttttatgcta taaaatttat tttatagcta ttaaatcaga
    13261 tgtttaaaaa ttttttaacg gtctatagtt tttaagagtc ttatagtaag tagcctaaaa
    13321 atatgatttt acatctgtac ttgttcgaga actccaagta taaaaaacta aggaattata
    13381 tagtggcagt atataattaa tacccttaga cacacctttt tgaaagtgga aaaaagatgt
    13441 gtagttctcc tcgaccacaa atcaaggagg ataaaaaatg gaaaagagaa aagtaataat
    13501 tgattgtgac ccaggaattg atgattcttt ggcaattctt ctggctttaa actcaccaga
    13561 gctagaagta attggaatta ccacatgttg tggaaatgtc ccagcaaata taggtgcaga
    13621 aaatgcacta aaaacacttc aaatgtgttc ttcactaaat attccagtat atataggaga
    13681 agaagcacca ctaaaaagaa aacttgtaac agctcaagat acacatggag aagatggtat
    13741 tggagaaaac ttttatcaaa aggttgtagg agctaaagca aaaaatggag cagtggattt
    13801 tataataaat actttatata atcatgaaaa agtatcaata atagcacttg caccacttac
    13861 aaatatagct aaagcactta ttaaagataa gaaagcattt gaaaatctcg atgagtttgt
    13921 atctatggga ggagcattta ggattcatgg aaattgctct ccagtagcag agtttaatta
    13981 ttgggtagac ccacatggag cagattatgt ttacaagaat ttatctaaaa aaatccacat
    14041 ggtaggttta gatgtaacta gaaaaattgt acttactcct aatattattg agtttataaa
    14101 tagacttgat aagaagatgg caaagtatat aactgaaata actagatttt atattgattt
    14161 ccattgggaa caggagggaa taattggctg tgtgataaat gaccctctag cagtagcata
    14221 ctttatagat agaagtatat gtaaaggatt tgaatcatat gtagaagttg tagaagatgg
    14281 aatagctatg ggtcagtcta tagtggattc tttcaatttc tataaaaaaa atcctaatgc
    14341 aattgttcta aatgaagttg atgagaagaa atttatgtac atgtttttaa agaggctttt
    14401 taaaggttat gaagacatta tagactctgt ggaaggagtg atatagtgtg aaaagaaaaa
    14461 tatgtgtaaa aactattaca cttatagctt ttggtatttc tataaatata ataggcgctt
    14521 ttatagctat gggattaaga ttacctgtat acttagattc aataggaact ataatgatag
    14581 cttcactttt aggacctaaa tacgctgttg ttacaggtgt gtttggaagt ttaataagtg
    14641 gaattacgtt tgatgtgtat tctctctatt ttgcaccagt tcaaatatca accggattac
    14701 ttgctggaat agcatttaaa aagaattttt taaggggttt aaaaactcca ctaggagtac
    14761 ttttatttgc cattccaaca tctataataa gttcaataat ttcagcattt ttatttggag
    14821 gtatgacctc ttcaggttca tcatatatag tgcaaatctt gaaagtgtta ggactggggg
    14881 atgtttttag tgtgtttgta acccaagtat ttactgatta tggagataag ctgttagcag
    14941 ttgttttagt gaatttagga ttaaatgcag ttccaaaaac tttaaaggta tcattgactg
    15001 gaggaaaata gatagaaaat ggatagatat agtgagataa caaataaaaa tcaaagagaa
    15061 attgttctgc ttaaaggatt tccttgtata tggggaaagt gttctttttg tgattatata
    15121 gatgataatt ctaatttaga agaagagatg aataaattaa atctaaaggt tttaaaaaac
    15181 gtaactggga aatatggagt tcttgaagtc ataaattctg gaagctgttt tgaattacct
    15241 aaggatactt tagaaaaaat aaagtgtatt ataaaagaga agaatataaa aaaattattt
    15301 ttagaaagtc attggtctta caaaaatagg cttaaggaaa tgagagagta ttttgaaatt
    15361 cctgtggtat ttaaaattgg agttgaaacc tttgacaatg attttagaaa taatatttta
    15421 aataaaaatg caaattttaa gactccacaa gatgtaaaag aatattttga ttcaccttgt
    15481 attatggttg gaataaaagg acaaacaaaa gagatgatag ataaagatat agagattatc
    15541 ctaaatactt ttgaaaaagc gacagtgaat gtttttataa ataattcttc tagtataaaa
    15601 agagatgaag agttagtgaa atggtttgtt agtaagtata aattcttgga tgaaaatcca
    15661 aatatagaag ttttatacaa taatacagac tttggtgtag gagattagca ttaatttagt
    15721 ttaaatttaa aattgaaaag tttcttatgt ttttgaaaaa gcttctaata gttagaagct
    15781 ttttttgtag agttttatgt tacaataaga aaaaaggaat taaggagcaa ttaaatgatg
    15841 tttgataggg gaaaatggaa taaattggat tataatgaac ttttagaata tatgatgtct
    15901 atacaagata taaaatatag agattttaat aaaaagctta tacctggaac agaaaatata
    15961 ataggtatta gagttcctaa tttaagaaag ttatcaaaag aaataagcca agggaattgg
    16021 aaggaatttt tagaagtagc acaggataca tactatgaag aagttaggct acaaggtatg
    16081 gtaattggca atataaatag taattttgaa gaaactttat actatgtaaa aagatttata
    16141 cctaaaatcg ataattggtc agtttgcgat ggattttgtt ctgatttaaa gtctatgaaa
    16201 aaatataaag aaaatatgta tgatatactt aaaaaatatg tttattcaaa gaatccatgg
    16261 gaaattagat ttgcattagt tatgttttta atatattatg ttgatgataa acatattaaa
    16321 gaaatatttg aatactgtaa taatatacaa agtgaagaat attatgttaa aatgggtatg
    16381 gcatggttgc tatcaatttg ttttattaaa tgtgaagagg aaacttttct ctatataaaa
    16441 aataataatt tagatgattt cacttataat aaaatgctac aaaaaataat tgaatcaaat
    16501 agagtagatt tagaaaagaa gaatataata agaagtatga aaagaaagac aagaagatgt
    16561 tgacggagga atattaatga ataacaatga agacagaatt gacaaatttt tagaacttca
    16621 aagcaaagga tttaaatttg cagagatagc tcaaaatcta aagattagtc aaagtacttt
    16681 aagaactttt ttaaataaaa aaggatataa gtcagaaaaa ggtaaataca tattaaaaga
    16741 cgaattaaat tataataaaa gtttggataa tagaaacttg gaagaagaga aagctaagga
    16801 gtatgaaaaa agtaaaaaga atactataag taatagaaaa aaagtaagca ccaaaaaaaa
    16861 gatttctaat gaaaaggctt taagtaaaag tgttgaacaa attgagttta atgatacagg
    16921 tttaaaaaat gcaaagccaa aacaaaatat taagcctaag aaagatagaa aaataaatat
    16981 aactcaagaa gatttagata agctttgtga agtatatgat tggtatttag aagtaaaaga
    17041 ttacaatata aagcctaaaa aagtatctaa taaaaaagat gtacatatag atgatgaaca
    17101 catagatgaa ttaaaaacaa caagaatacg tatagataaa gaaatatgga atgattttga
    17161 aagattatgt agtaattcga attttgaaaa acatcaaata cttacacagg cattgaaaga
    17221 atttatgaaa aagtataaaa atctactata aaatactact atatgaggtg cttaatatga
    17281 ataaaatagt aaaaaaatca atttctctaa tgattatatt aacaatattt atttttatgt
    17341 tgacagcatg tgaaaaagat gaacagcctg attcagttga tattcaaaca gaagataaaa
    17401 atgagataaa aatagatgag ggaaatgcaa aagtattgaa tataggaaaa tcagaaatta
    17461 taaatataga tgaaggtgat aaagtagata catcaacaaa ggtagaaaat aattcaacat
    17521 ttaatatatc caatgttgaa ttgatatata atgaatatga tgagaataaa aaaattatat
    17581 cttcggatag taaggctttg ttggacatga ctttaatgcc tgggaaagtt gcttacatag
    17641 aatgtgggca taaaactttt gtaaagggtg tagaagttta tgcatatgaa tatgaggcag
    17701 agggtaagat tgtgtatgtt aatttaaaag aaaatactat taatataaga aataataata
    17761 ttaagctaga aaatagtagt cagtatgagg tgctatctac atcagaactt aaaaaagtaa
    17821 atgaatcaaa tgagggaatt acgtatcaag taaaagtaaa aaattcatca tcaaaagatt
    17881 taggaaatat tattcttaag acagcagaag ttaatgaaaa tggaaaatat ttaacagtaa
    17941 atcgtgtacc atcatataag attttaaagc cttcagaaga gacagatata gatatattgt
    18001 gttcaacaaa ggcaaaaagt gttgaaatag tgggatatac gtatgatgat ataaaagaaa
    18061 aagccaatgt ggatattgac ttaaaatctc ataaagttaa gatagacaaa taaaggtagg
    18121 ggatacttat gaaggtttta atagttgaag ataataagat acttttagaa agtgtagtag
    18181 aagaacttag caagcatttt gaaactgaaa agtgtgaaga tggagaagaa gctttatact
    18241 taataaatca aaatatatat gatttagtca ttttagactt aatgcttcct aatataaatg
    18301 gatttgatat cctaaagaaa atgagaatta ataatataga tacacctgta cttatattga
    18361 cagcaaaaga aacattagat gacaaagtag aagcatttac tataggagct aatgattatt
    18421 tgacaaaacc tttttatatg gaagagttag tagctagggt atacgctata ttaaggacta
    18481 atggaaagat aaaagaaaga aacggattag aatttaaatc tttatattta gatacactag
    18541 aaaaaagagt gtatattgaa aaagaagaaa taaaacttca aaacaaacag tttaacttgc
    18601 ttgagtattt tgtattaaat aaaggttcaa ttttattaaa agagcagatt tatgatagaa
    18661 tatggggaat tgattcagat gcaactattg aaatagtgga agtatatgta agtaatctaa
    18721 gaaaaaagct aagtaaatat ggatatgata aatatattaa aactaaacgt aaggtaggat
    18781 atatattcga tgataaataa gaatgtattt accagtacta aaaatcattt aataaaaatg
    18841 tatattattg tagtaggtag ttttttaata atcttttcta tatttatata ctcatatttt
    18901 aggggactta catatagtgg aattgatagt gagataaatg atgaacttga atatatagtc
    18961 tctcaattta aaagaacttc atttttgaac ccaataagac tcaaagatcc taaagatatg
    19021 gtgtatgtat atgaagatgg aaggataagt tactatactc aaaatgaata ttttgatgag
    19081 ttacttccag atagaagact agataaaaaa aattcgtttt ttaaatacac agaaaatgga
    19141 tatactttta gagaattaaa tgtagatgta ggtagatacc aaatacagat aattagaaat
    19201 atagattctg agatgaattc tttaagacag cttacatctg ttcttataat tggaatatta
    19261 atttctgtaa taattactta ctttgtggct gtatatctca ctagaaaagc tttaattcca
    19321 atagaaacag catggaaaaa tcaagctaag tttatacaag atgcttctca tgaattaaga
    19381 acaccaataa caatagtttc atcaaaatta gaaagtatgt taaaatcccc agaaagtaca
    19441 gtaaatgatg aagttgaaac aatagctact gcaatgaagg aaactagaag acttaaaaaa
    19501 atgataactg atttattgtc tcttactaaa gaggactcta ttgttaaagt taatttagaa
    19561 gaaatagatt tagaaaagtt attggaagaa atatcagaag actatattga cattgctgag
    19621 tttcaggaaa aaagattcgt gtttaattct aaactgaaga ataaagttat aataacagat
    19681 aaaaataaat taagacaact tatattaatt ttcattgata atgcctttaa gtatacaaaa
    19741 ttgggtgatg agatagcatt agaattgaaa gaagatatag aggatgaggt aactttactt
    19801 ataagtgata ctggtatagg cataaaaaaa gaagagatac ctcttatatt tgatagattt
    19861 tttagaagtg aaaatgtaag aaataaagat ttagaaggtt ctggaatagg actttcaata
    19921 gctcgcatga ttagtttaaa cttatctatt gatataaatg taacttctga tgtagatatt
    19981 ggaactacat ttgaattgtc aataccaaaa aagttaaaat aataaaaata taaaaaattg
    20041 tttttagaaa tgaagaatgg gtatactaat tagtataaaa tataaaaaga ccttataaaa
    20101 gtaaatttat atttataaat ttcatagtat aattatatag ggataataaa gtttaatagg
    20161 aggatttaaa aatggcaaaa gttataaata catctgaatt tagaagtagt gttgaaggta
    20221 gtaagggagt agttgtagtt gatttctttg caacttggtg tggaccttgt aacatgttgg
    20281 gacctgtatt tgcagaatta ggtgaagaga tgaaggataa agctagattt gtaaaagttg
    20341 atatagatga gagtttagaa atagctcaac aattcaatgt atctacagta ccaactatga
    20401 ttatatttaa agatggaaaa ccagtagaaa ctttaattgg attcatgcca aagaacaaaa
    20461 ttgaaatgca agtaaaatca tatttataaa aaatgggtga gtattatgag atatgatata
    20521 gctataatcg gcagtggtcc agctggattg tctgctgcta taaatgcaaa aataagaaat
    20581 aaaacaatta tcatgtttgg taatgataat ttaagtaata agcttgttaa agctccttca
    20641 atagataatt atttaggttt ttatgatata agtggtgatg aattaaaaga caagtttaaa
    20701 tcacatatag atagtatgga tatttcaatt gaaaataaaa gaataaataa tatttatgct
    20761 atgggagaat actttacaat aatgtctgga aatgatatgt atgaagctac tactgtaatc
    20821 cttgcaactg gggtagagta tactagacca atcaagggtg aagaagaatt tctaggaaga
    20881 ggtgtgggct actgtgctac atgtgatgca ccactttata gaaataaaaa ggtagcagtt
    20941 ataggatata atgaagaatc taaagaagaa gcaaattttt taagtgaact cacttcaaag
    21001 acttatttta tacctatgta caaaaaagat aatcttatga gaagttcaga taacctagat
    21061 gattctattg aagttataca tgacagacca gtacagatag atggagataa acttgtaaac
    21121 aaagtatctt ttaaagaaaa ccacattgaa gttgatggag tattcgttat aaaagatagt
    21181 acggctccaa gtgcattggt tcctggaata gagattgatg gcattcatat taaagttgac
    21241 aataatatga agactagtat agatggatgc tttgctgctg gagattgtgt tggaaaaccc
    21301 tattcttata taaaagcagc tggtcaaggg caaatagcag ctttaaatgc tgtttactat
    21361 ttagataaat taaagagggc ttaatattta tattgattaa ctttttaaag atatttataa
    21421 aaaactgtct tgaaataatt ttattatttc aagacagttt tttattttta agttattaat
    21481 aaaagtattt taaaatctat atgtaattac ttttattaat aaaactcaaa gttatattag
    21541 taaaaattgg aattatttat aatatactcc aaaatttaaa aataatggtt gaaaaatcta
    21601 attaagagta ctataattta attaagtaat tacttaatca aaatgaggta cttatggaaa
    21661 acttggttaa aatatttaaa gcattgagtg atgaaactag acttaacata cttatattag
    21721 tatccaagag aaatatatgt caaaagggga tttctaaata tctagggatt tcagattctg
    21781 ctgtttcaca acatataaaa atattaaaag atgttgggat aataacagga tataaagaag
    21841 gatactatgt tttatatcat attaataaag aatcatttaa tatatgtgta gattttataa
    21901 attctatgtt aagtaattct agtgaaagct ttatagatgt atttgatgta aatacaatac
    21961 atctaggatg cagtaaagaa tgtaaatcta taaaaaaatg ttgtaagaga agggagaaat
    22021 aataatgaaa gtatgtatac cagtagagga gaataaagga ttggatagta aaccatatgg
    22081 acattttggt tctgctccaa tttttgttgt gtgtgattta gaaagtggag aagttaaatc
    22141 tttagataat ggagatttag atcatgaaca tggaaaatgt caaccattaa aagcattgtc
    22201 agggacagct gtagatgcag tagttgttgg aggaatagga caaggagcta taattaaatt
    22261 aaatggaatg ggaataaaag tttacagagc agaaggtgat actataagtg ctaatttaga
    22321 cttacttaaa aatggaaaat tagtagaatt tccatcagac catacttgct cacatgacgg
    22381 atgtggacat cattaaaaaa aaacagctga tgaaatatat acttcatcag ctattttttc
    22441 ttttaataaa tacgatttta ttcttttaaa accaaaattt agatttttat gttttttgta
    22501 gtatccttaa taatatttca agtttacaag tttactactt ttagattttt tttgtatgtt
    22561 tttctatata gtcaatatca tttaatatgt cattgagttc tttattaagt gtttttaaat
    22621 catcaccatc tacattgtag tttaaaaata gttcctctac attatcaatg atagaattga
    22681 cttttctagt aacttcaaca aatgtcttaa agttactact atttttagca tttagaaatt
    22741 tttttaaatc ctttgatata aatgatacta tttcatattc atcattaaat agacctctac
    22801 caataggata tggttcaatt ctctgtaaaa aatattggtc tttagcatta atattataag
    22861 tataagatat ttcccaaccg tctcctaaat caaacttgat aaaatataat gtactactta
    22921 cttttgatat tcttgtggca ccaagctgtc ttaaaagaac ttcaatcgaa tcaccttgaa
    22981 cagtattaat attcatatat acacatcccc tttagtatta ttatactata ttttttaaaa
    23041 ttttataatt aaataaacag tagatataat aatagatata atcataagtg gaaaacctat
    23101 tttgaaatac tccttaaaag agataggatg accgtgttta ttacctatac ctgctagtac
    23161 aacattagca gatgctccta ttagtgttcc atttcctcca agacaagctc caagagatgt
    23221 tgcccaccat agtggcatta catcaatacc ttcagcttgc atagtcaaaa taagaggaat
    23281 tagtgttgca acaaatggaa tattatctaa gaaagaagag actatagctg acaaccataa
    23341 tatcaacaac attgtaaata caagatgtcc ttctgtaagt ccaatcaatg cttgagccaa
    23401 tttgtttatt atcccaactt ctactaatcc accaactact acaaataatc ccataaagaa
    23461 taaaattgta gaccattcaa tacctgccat aatttcatct acatcttgtt ttcctattaa
    23521 aagcattata gatgctcctg tcagtgctac aacagaagaa tctatgtgga ttgtactgtg
    23581 aaacataaaa ccaaaaagta tgaatgctat taatataagg ctttttataa gtagaggttt
    23641 atctttaaca gatttttttt catctagttt taatattgca tttttagctc tttcattcac
    23701 aacaagttct tttccataaa taaatctaaa gcatattata gttatagcaa gtattactat
    23761 aacagcagga cctaaattta ttacaaaatc cataaaacta agattggcag cactaccaat
    23821 cataatattt ggagggtctc ctataagagt agctgttcct cctatatttg aagctaaaat
    23881 ctgtgttatt aaaaatggaa ctggattaag tcctaatatt tgtgtaataa ctatagtcat
    23941 tggtccaata agtaagacgg ttgtaacatt atctaataca gctgataaaa tagctgttat
    24001 aatagcaaaa caaatcatta tcttccatgg gtcacctttg gctttctttg cagtccagat
    24061 agctatatat tcaaataacc ccgagttttt gacaacagct acaaatagca tcataccaac
    24121 taatacacca atagtattga aatctatatg gcttaatcct tcatcaagtg taagtatatt
    24181 gaatattatc attaatagtg caccaccaac agctgctacg gatctgttta ccttttctgt
    24241 aataatagct gccataacta ttaaaaatat acatatagct attatttgtt gaaattccat
    24301 aatataagta cctccatttg tattattttg tgcctaatgc tagtactata tatagtatta
    24361 cttttttatt gttttttcaa tggatgtatg aaagtatatt atttaacaaa gtttttggaa
    24421 aattgacaga atttaaagaa aatttttgtt atttaaataa attttaaaat tattccattg
    24481 ataataatat taaacgacaa acgaaaaaat atac
//
LOCUS       DAMJZE010000037        24469 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJZE010000037 DAMJZE010000000
VERSION     DAMJZE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.037"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="PC541_001407"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="PC541_001407"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="PC541_001408"
     CDS             275..466
                     /locus_tag="PC541_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2838877.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="PC541_001409"
     CDS             621..2030
                     /locus_tag="PC541_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2838878.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="PC541_001410"
     CDS             2161..2850
                     /locus_tag="PC541_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2838879.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="PC541_001411"
     CDS             2853..3788
                     /locus_tag="PC541_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2838880.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="PC541_001412"
     CDS             3785..4678
                     /locus_tag="PC541_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2838881.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="PC541_001413"
     CDS             4756..5502
                     /locus_tag="PC541_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2838882.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="PC541_001414"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="PC541_001414"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2838883.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="PC541_001415"
     CDS             6359..7063
                     /locus_tag="PC541_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2838884.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="PC541_001416"
     CDS             complement(7251..7679)
                     /locus_tag="PC541_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2838885.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="PC541_001417"
     CDS             complement(7716..9146)
                     /locus_tag="PC541_001417"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2838886.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="PC541_001418"
     CDS             9278..9862
                     /locus_tag="PC541_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2838887.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="PC541_001419"
     CDS             9878..10399
                     /locus_tag="PC541_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HDF2838888.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="PC541_001420"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="PC541_001420"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2838889.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="PC541_001421"
     CDS             12762..13532
                     /locus_tag="PC541_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2838890.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="PC541_001422"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="PC541_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2838891.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="PC541_001423"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="PC541_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2838892.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="PC541_001424"
     CDS             14894..16009
                     /locus_tag="PC541_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2838893.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="PC541_001425"
     CDS             15999..16868
                     /locus_tag="PC541_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2838894.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="PC541_001426"
     CDS             16861..17727
                     /locus_tag="PC541_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838895.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="PC541_001427"
     CDS             17768..18481
                     /locus_tag="PC541_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2838896.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="PC541_001428"
     CDS             18482..19651
                     /locus_tag="PC541_001428"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2838897.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="PC541_001429"
     CDS             19655..20491
                     /locus_tag="PC541_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2838898.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="PC541_001430"
     CDS             20492..21487
                     /locus_tag="PC541_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2838899.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="PC541_001431"
     CDS             21505..23193
                     /locus_tag="PC541_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2838900.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="PC541_001432"
     CDS             23205..24272
                     /locus_tag="PC541_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2838901.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9758 a         2749 c         4377 g         7585 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaa
//
LOCUS       DAMJZE010000038        24449 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJZE010000038 DAMJZE010000000
VERSION     DAMJZE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.038"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(52..888)
                     /gene="nadC"
                     /locus_tag="PC541_001433"
     CDS             complement(52..888)
                     /gene="nadC"
                     /locus_tag="PC541_001433"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2838902.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(869..2170)
                     /locus_tag="PC541_001434"
     CDS             complement(869..2170)
                     /locus_tag="PC541_001434"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2838903.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(2183..3097)
                     /gene="nadA"
                     /locus_tag="PC541_001435"
     CDS             complement(2183..3097)
                     /gene="nadA"
                     /locus_tag="PC541_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2838904.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            3515..4972
                     /locus_tag="PC541_001436"
     CDS             3515..4972
                     /locus_tag="PC541_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2838905.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            5195..5623
                     /locus_tag="PC541_001437"
     CDS             5195..5623
                     /locus_tag="PC541_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838906.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            5683..5991
                     /locus_tag="PC541_001438"
     CDS             5683..5991
                     /locus_tag="PC541_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2838907.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(6138..7160)
                     /locus_tag="PC541_001439"
     CDS             complement(6138..7160)
                     /locus_tag="PC541_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2838908.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(7335..7841)
                     /locus_tag="PC541_001440"
     CDS             complement(7335..7841)
                     /locus_tag="PC541_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2838909.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(7901..8602)
                     /locus_tag="PC541_001441"
     CDS             complement(7901..8602)
                     /locus_tag="PC541_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838910.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(8820..9890)
                     /gene="buk"
                     /locus_tag="PC541_001442"
     CDS             complement(8820..9890)
                     /gene="buk"
                     /locus_tag="PC541_001442"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2838911.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(9943..10518)
                     /locus_tag="PC541_001443"
     CDS             complement(9943..10518)
                     /locus_tag="PC541_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2838912.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(10520..12307)
                     /gene="iorA"
                     /locus_tag="PC541_001444"
     CDS             complement(10520..12307)
                     /gene="iorA"
                     /locus_tag="PC541_001444"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2838913.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(12368..13627)
                     /locus_tag="PC541_001445"
     CDS             complement(12368..13627)
                     /locus_tag="PC541_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2838914.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(13889..15889)
                     /locus_tag="PC541_001446"
     CDS             complement(13889..15889)
                     /locus_tag="PC541_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2838915.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(15897..17784)
                     /locus_tag="PC541_001447"
                     /pseudo
     CDS             complement(15897..17784)
                     /locus_tag="PC541_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(17811..19652)
                     /locus_tag="PC541_001448"
     CDS             complement(17811..19652)
                     /locus_tag="PC541_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2838916.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(19783..20202)
                     /locus_tag="PC541_001449"
     CDS             complement(19783..20202)
                     /locus_tag="PC541_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2838917.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            20515..20697
                     /locus_tag="PC541_001450"
     CDS             20515..20697
                     /locus_tag="PC541_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838918.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            21026..22048
                     /locus_tag="PC541_001451"
     CDS             21026..22048
                     /locus_tag="PC541_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2838919.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(22141..22743)
                     /locus_tag="PC541_001452"
     CDS             complement(22141..22743)
                     /locus_tag="PC541_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2838920.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(22760..23908)
                     /locus_tag="PC541_001453"
     CDS             complement(22760..23908)
                     /locus_tag="PC541_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2838921.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            complement(23901..24278)
                     /locus_tag="PC541_001454"
     CDS             complement(23901..24278)
                     /locus_tag="PC541_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2838922.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT         8015 a         4178 c         2599 g         9657 t
ORIGIN      
        1 taaaacaaat ttaaggagtc tattttaatt aagtttattt caatataaaa tttatatatt
       61 tctcaaattc ttcatactta tatcgagagc tttaacagaa tgtgtaagcg ctcctacaga
      121 aacaacatcc acaccaattt ttcctatatc ttctatagta tctaaattta catttccgga
      181 aaattctaca atggctcttc catttataat atctactgct tgttttgcca tttccaaact
      241 catattatcc aacattatta tatctgcatt agcttctatt gcttctttta ccatgtctaa
      301 agtttccact tccacttcta tctttctaac aaaagaagta ttttttctag ctaattcaac
      361 tgccttctta acactaccag cagccccaat atggttgtct ttaagcaata ttccatcaga
      421 taaattaaat ctatgattgc atcctccacc tactttaaca gaatacttat ctaatattct
      481 aagattaggt atcgtttttc tactatctaa taattttgta tttgtacctt caagcttttc
      541 aacataccta cttgtaatag tagctatacc actcattctc tgtaaataat tcaatgctac
      601 acgctctcct acaagtaagt ttctagtact acctgttaat acagctatct tttcacgatt
      661 ctttactcta tcaccatcat ttttatataa ttttacatct acatcaccaa gtatctcaaa
      721 aactctctta aatacaccta agccagctat tataccatct tgtttacaaa ttaaatctac
      781 agtagattta gaattttcat ccacaataga attagttgtt atatcttcac ttggaacatc
      841 ttctatcaaa gcatcctgta tcattctatc aataatcaaa taattcatcg ccaaaatcct
      901 cacttgttct ttcaatttct tttatgacta atattttatt ttcttttttt acctgttcta
      961 tatctatatc catatctggt acatctataa acttaacttt tacattatct ataactgagt
     1021 ttatattttt agctgctctt tttgaaaata caagcccttc aagtaatgaa ttacttgcca
     1081 atctattggc tccatgaaca cctgtacaac taatttcacc aactgcatat aaattttcca
     1141 tagatgtttt tgaatccaaa tctacatgta tcccgcccat aaaataatgt tgtgcaggag
     1201 atacctttat acattctttc gttatgtctg ttcctctagc taaacattca ttataaataa
     1261 aagaaaatct attaattaaa tattcactat ctaaatgtgt tgcatctaaa tacacataag
     1321 gtaatttatc tttttccatt tgctcaaaaa tagcttttga aactacatct ctaggaagaa
     1381 gttcatccac aaatctttct ttatttttat ttaaaagttt agctccctct cctcttaatg
     1441 attcagatat aagcattctc ctaccttcat tattttcttc ataaaaggct gtaggatgta
     1501 tttgaatata ttctaaattt tctattttta tattatttct taaagctatt gcaattccat
     1561 cacctgtaag atgtctttga ttagtagaat ttttaaaaag cccaccaatt ccacctgtag
     1621 ctaaaactac tatttttgaa aaaacatgaa tctctttttt agatttaaaa attctagctc
     1681 ctatacattt attgccatct tttaatatat ctaaaagata ggcatcttct ataagagtta
     1741 tattttctct agttttagct tcttttaata aagttttaaa aacaacttct ccagtattat
     1801 ccttactatg aactattcga tttacactat gtgctccttc tcttgtgtaa tctatctctc
     1861 cattttcttt gtctaaaggc ataccatatt ctactatctg ccctacattt tctatagatt
     1921 catctgcaag tacttgaact gcttcaactc tatttttata ctgcccagcc ttcattgtat
     1981 cttctacaaa ggattctata tcatcaatat ttctagcagt tgaaattcca ccttgtgcaa
     2041 gatatgtatt gttgttttca attgttgatt tcgatactac tagcacattt aaacttttat
     2101 ctaaatttaa tgcacagtat aatcctgaaa ctccacttcc aactattaaa acatcttgtt
     2161 ctaaattcat aagtctatcc ctctatcctt ctgctaattt gtgcatgtta agcagtgaat
     2221 ttaaagcctt ttccatgatt tcatcttcta caataacttc attttccata tttttaagtg
     2281 tactatataa attttctaat gttgtctttt tcatatctgt acatagtatt tttccaccag
     2341 gaatataaaa gtttttatta ggattatttt tctttaattc atgtaatata ccttcttcag
     2401 ttgctattat aaagtctttg tttttactat tagtagcata ttttattatt cctgatgtac
     2461 tacctacata atctcctaaa tctcttattt cttttttaca ttctggatgt actaatactt
     2521 ctgcattttt atacttatct tttaactcaa ttatttcttc agctcttata ttgttatggt
     2581 atttacaata tccgtcccaa aatataaagt ttttatctgg aaattgttct gcaatatatc
     2641 ctcctaaatt tttatctgga aggaatataa tttctttatt atctatgttt tctagtattt
     2701 taattgcact agaagatgta acagaaacat cacaatgtgc cttaacttta gctgtagagt
     2761 ttatataaca tacaaccaaa gcatttggat actgttgttt taattttatc acgccttctt
     2821 catctaccat atctgccata gcacacccag cgttacttac tggcatcaat acagtttttt
     2881 caggtgataa tatcttagca ctttctccca taaaccttac tccacaaaat acaaccactt
     2941 cttctttaca atctctagca atctcactta aataatatga gtcaccaact gcatctgcta
     3001 attcttgtat ttctggtggt tgataaaaat gggcaagtat tattgcattt ttttctttct
     3061 ttagctcttt gatttgatat gttaaatctt tatccataat tgcaccgctt tctatatgta
     3121 ctagtgtata tacacctgta aactcattat atcacttgac tattctttct tcaagaattt
     3181 taaacctctt aatattgtta agatatagac atttatattc tcaattgatt ttcctttttt
     3241 ctccatctat ttaaactaat ttttatattt tttaattttt ttgtaaaata ttatcagaat
     3301 tttgtctata ttctttattg ctataacatt gaaaatatta ccaaatacct gtatactaag
     3361 tacatggtta tgtatatgta tttatttctg aaattatatt agttgtaata ctttatagaa
     3421 tttgtaggtt gtccagaatt aaattcaata taaatctaca catttagata atatgtatac
     3481 aaagaccaaa aaattttata aatggggtga tattatgaca acattcttaa tcggcttggc
     3541 tatattacta ataggaggtg ctttatacgg tgcttattgt gaaaaagtat ttggcccaga
     3601 tgacagaaaa acacctgcac ttgctcaaag tgatggagta gactatgtcc caatgaaaaa
     3661 atggaaaaat agtctaattg agttgcttaa tatagctggt acaggaccaa ttctaggacc
     3721 tatccaaggt atattgtttg gtccaatagc attcatacta atacctattg gctgtgtatt
     3781 tggaggagct ttgcatgact atatgagtgg aatgattagt ataagagaaa aaggagcaca
     3841 aatgccttct cttatcagta gatttttagg taataaagta tttcaagtct ataatatatt
     3901 cctttgtctt ttaatgcttt tagttggtgc agtttttatt tatacacctg gggatttagt
     3961 tgttactcaa attttaaata tgaaatcgac tataaataat ccagttgtct ggatagttta
     4021 tggacttatc tttctatatt acttgtgtgc aacactattt ccaattgata aaatcatagg
     4081 aaaggtatat ccaatatttg gagctattct acttttatct gctgctggtg ttggaattgg
     4141 aatctttaca caaggttatg atttagctaa tttaagtctt gcaaattgga aaggtattca
     4201 tcctgatggt attcctctta ttcctacatt ctttgttaca gttgcttgtg gtattgtttc
     4261 tggattccac tcaactcaag ctactcttat agcaagatca gtttcaaatg aaaaagaagg
     4321 aaaaactact ttttataata tgatgatttt agaaggttta attgcaatga tttgggctgc
     4381 agctgctatg ggtatttaca ataaaggaat acctaaggaa ttagtaggtt ctcctgatgt
     4441 aataggtttg gttgcaagag atttacttgg ttctattggt ggtatcatag ctataatagg
     4501 tgtaattgtt ttaccaataa cttctggaga tacagcttta agatctttaa gattaatgct
     4561 tgcagattac tttcattatg accaaaagga aaaaaaacat cgtgttatat tgtcaatctg
     4621 tatatttatt cctgtcatag ccatccttat atttgcaaag cttagtgctt ctggatttaa
     4681 tatattgtgg agatacttct cttggagtaa tcaaactatt gccatatttg catttgcaat
     4741 gattacagtt taccttataa taaaagaaaa gaattatatt ataagcttaa ttcctggtat
     4801 gttttattcc tttgttatat tcagttatat atttaatgct caaattggat ttaacttaaa
     4861 cataaatata tcatatgttt tagctgccat atttactgta ttatatgcta ttttaactgt
     4921 acgctctggt agaaagttaa aaagcaaagc agatactaag ttggctgact aaatctaaat
     4981 aaaataatac ttagaataaa ttttattaaa aaatgataaa aaagtaggca atctcaagtt
     5041 agatttgcct cttttttatt taatacagta ataaatactt aagtatttac tccttattgg
     5101 aatatttatc ctaaatctct aatataaata aataaaagtt ttaaaataat ctattcgttt
     5161 atatacttaa aataaatttt ttggaggaat acttatgaat aataaattac atgaacttga
     5221 aaaaaatcta ccagaaacat cttgttcttt ttgcacacat ctttcattta aggggcctag
     5281 cctagactat agatatgata ttagatgtgt tatttcagat acaaagcccg attttagagg
     5341 atgttgtgaa tattttgaac cagaatatac agaattaaat acaggagatt tagacaatct
     5401 atacataaac tttcttgaga catgcctcaa ggtaaattat aatgattaca tcaattctat
     5461 gtattggaag ttatttaaag aaagaaccct actagaaaac aattttaaat gttctatatg
     5521 tggatcaact gaaaatgttg atgtgtatca tgttaataaa aatttaggaa gggaaacttc
     5581 caaagatgta gttgttaaat gtgataaatg taattgtaaa taagtgtata aaatttagat
     5641 aacttcacat aataagttta aataaaactt aggaggttat ttatgaaaaa taataattta
     5701 aattgtgctg ctactaattg tgcttataat actagtggat attgttatgc tggtagtata
     5761 aaagtagatg gaatgcaagc aactactaca gaaaatactt attgtgcttc ttttgaagac
     5821 aaatatacat ctggtataac tagtcgttct aatgaaacta accaagttga tacagataac
     5881 attcattgtg aagctgtaaa atgtaaatac aataaaaatg aactttgcaa agctgaaaaa
     5941 gttcatataa atgaaagaaa tgcaagttgt gaaacttttg aaatgaaata aatttctttt
     6001 tatttagttt tatagtattt atacaaagca aaattgaacc tatcaaatag atgtctttat
     6061 taatgatgtc tatttatagg ttctttttta aaacaattta taatttatat ataaagataa
     6121 attattattt taatatatta cctagaagta aatttaacac ccagaatatc cataaggaac
     6181 ataaatattg gaactccaat tagtaatccc catacaccta aataatgttc tcctacaagt
     6241 aaaataataa aaacaaaaca aactggaagt ttagttctat tcgacatcaa cttagggttt
     6301 aatatgtatg tttctactgc atgcactata caaatcataa gtaaaactcc aaaaaccttt
     6361 gttattcctc ctgtattaaa tgctattaca gttaatggaa tcaaagacac tataactcct
     6421 gctactggaa taagacccaa cacaaaaatc ataaatccta aagcccatat ttgagggaat
     6481 cccatgaaac ctaagaaaat catagaaaca acagaattta taaaggcaat cataacttgt
     6541 actttcatta cttcaccaaa atttttaaca aagctctttc caaaaaatac aagcgaatta
     6601 taaataaaag aaatcttact atcagaaagt ttatctccaa aatttttaat ttcatttttt
     6661 tcaagtaaaa ggagtaaact cagtaaaaat gccaataata tatttactcc aaaacttcca
     6721 acttttgttg ctgttgatgc tataagtagc cctgcttgtg aaatatattt attaaaatcc
     6781 agctttgaga caataggata caattttggt cctaaagatt gagcaaattg atttacatca
     6841 aaatcactaa aaaccctact caactctgta agttgaacta taattattgg tacaactgca
     6901 tagctaatca atactaacaa tatagcaaat agtgcatata acactataat tattaatgaa
     6961 tctggaatat ttaaagaaga aaacttctta attcgttttc ttgtggctac aagcaagtta
     7021 tagaaaataa aagtcataat aaatgttaaa agcaccatat ttaacatata ccaaaatgta
     7081 attagaataa ttactacgac agatatagaa acaattttat attttgaact gtcacgtgcc
     7141 aatcctttgt tatcttccat agtttaaatc ctccccaaat ttttatgact ttataatgac
     7201 tgcccttttt attttaatct caaaaaacaa gtagaaaaat actaaaaata acttattaat
     7261 tttcataaaa caatattaaa atttagagaa gatacatttt aaataatctt acttatactg
     7321 aaatctgctc tacatcatat atttaattta ttattatcta tctcatatag caattcgtat
     7381 atctctggat attcaaaaaa caaaccctct tttagtaatt gtcttatttc atctgtgctt
     7441 acccacttta tatcacttac ttcttcctcc tgtaaaatag ctttggatat atcatattct
     7501 tttttaacta agtatacatc ccaaagagta tcttcatgaa tcatacttct aaaaactttc
     7561 atctcatctt tagttatatc tatccctatt tcttcctttg cttcgcgaat tgctccttct
     7621 aaactgtctt ccccagaaac aatacatcca gttgtcattc cccacatatt tggcaatgtc
     7681 tttttacttt tagacctttt ctgaattaaa atttgagagt tactatttaa aatccacact
     7741 tcaactgcca aatggtaata accttcttca atagaattac ctctttttat aacatttcct
     7801 gttttaattc catcagcatt atataaatcc cataattcca tttcaaaatc tccttgactt
     7861 tactataaat tagataaact acaaatatat tttagcataa ttaattctgc tcatttttta
     7921 ttacaatact tgatttacat ttatgttcat tgcttttctt ttccttccat tctcttatat
     7981 catctataat aaatctatgt ttcaacaatt ttgtgtcaaa tttaaatatt ctaaacacta
     8041 tttgtaatac atatcctaaa cctaaagcac ttacaagcgt accaatacct acagaacctc
     8101 ctaataacca cccaacaact aatgctagta tttcaagaat tgttctaaca agacgtacag
     8161 atttattagt ttttttctga agagcaatca taagcccatc tctcggtcca cttcctaaac
     8221 aaacacctaa gtatagaaaa cttgctatag ctgccaaaat aattcctata acaaccataa
     8281 acacacctat ataagtgttg cttgcatgag gtatcactcc agaaaatatt actaaatcaa
     8341 taaaaattcc tataaatgtc atgttgagta aagtacccca tcctatattt tcaccaaaaa
     8401 ctgcatccaa tataacaatt acaactccta ccattataga agcctgacct atagttatgc
     8461 ctagctttat agataaacct tggtgaagta catcccaagg cataagacct acatgtgcat
     8521 taatcataag cacagaagaa attgcaaata taaaaaaacc tgaaaataat cttatcatac
     8581 tttttaaact tatttttccc atcaaaaatc actcctcata ataataataa caataaatac
     8641 caataaaata tatatataaa tacgaaattt tacagaatta tttattaggt taagataaaa
     8701 ttttaattat aaaaagtggc aatccaaatt ttatacattt tcacctatac tctaagcttt
     8761 cgtgtataaa aaacggatat accacttttt tagtatataa acatttttag tttttcttat
     8821 taattttcac tataaattct agcttcttct tctccattta gtactcttaa agttccaaaa
     8881 gcaagtgatt ccaactcatt ttctcctgcc ataatttcta ctggtgctat aaactcaact
     8941 ttctttttaa ccattgaagt aaacatttca gaatatgcaa ttccacctgt tataattata
     9001 gcatctacct ttccatctac tacagttgct aactctccaa taccttttgc taactgataa
     9061 gccattgctt cataaataat ttttgcttct tcatttcctt ctgctatcat tttttcaact
     9121 tctctagcat ctacagtatt taggtatgaa actataccac ctttacctct tatcttttta
     9181 gtcatttctc tttctgaata ttgacctgaa taacaggcag ccactaattt tgtagcaggt
     9241 actcttcctg acctttctgg agaaaatggt ccttcatcat cagatagcat atcaaccatt
     9301 cttccttttt catgaagata aatacttact cctccaccaa tgtgagctac tattaagttt
     9361 aagctcttat aatcttttcc tttatcctta gcatatttta aagccattgc tctagtattt
     9421 aatgcatgcc ctgcacttga tctatccatt ccacaaagtc cagatattct cgctacatct
     9481 ataagctcat ctactgcaac agaatcataa atatatgagt tgattccaag aggttctgat
     9541 attgcatgtg ccacaacagc acctaaattg gaagcgtgtt caagtactgg tctatgtctt
     9601 agtacatcaa tcatttcttc atttactaaa tatgctcctg attttactgg tggtaaaact
     9661 ccacctcttc ctactattgc actaagagat tttaagtcta tactattttc tttaagaata
     9721 ttcaaaactg cttccttacg catttcaaac tggtcttgta tagtattata ttttgcaatc
     9781 tcttcagctg gatggtctat cgtcttcaca agaatttgtt cttctccatc atatactgct
     9841 atttttgtag aagtagaacc tggatttatg gctaatattc tgtaagtcat aattttcttc
     9901 ctttcacctt aatttctata gtctatttat atctattata aactatttgc taaccatttc
     9961 cataccttca tggaaagctt ttatgtttaa ttcaacaaat tttggtttaa cattttcact
    10021 tataattttt tcccaatcaa tagactctaa ttgcatagat ttaactagac atcctaataa
    10081 aataacattc attatttttg aatttcctag ttcttcagct ttatcagctg catttataac
    10141 ttttgcattt aatctactta actctgcatc tatatcactt tcttgatatt cagctcctcc
    10201 gattaaaaca gtcatagaat ctatcctatg attattcact aaagcttttc cttctggttt
    10261 taaataattg aaccatctta aagcttccat tttttcaaaa gatactaata tatctgctcc
    10321 accaatttct ataactggag aatgaacaca atctccatat ctaacttgtg aggaaactga
    10381 acctcctctt tgactcattc catgtatttc actcatcttt acatcatatc cagcttccat
    10441 caatcccata gttaataatt tactagcaag tatagttcct tgacctccta cacctaccaa
    10501 aagtatactc ttagtcatat tatttttcct ccttaactat tgcttgtttt ggacaaactt
    10561 gtgcacatac tccacatcct acacactgat ttctatctat atttgataac ttattctctt
    10621 tatcaaatga tagagctgga catccagttt tcaaacataa tttacatcct atacatttat
    10681 catggtcaac tttacactta gtcttaaatg gattattaaa ttcttctata tcttctttag
    10741 agaatttttt aagtacacat ggccatcttg ttattattac tgatggttca tcttctatag
    10801 ctaaagccca atctagtgct tcatttactt cttttaaatt attagggttt ataactctta
    10861 catgttctat tccacaagct ttaactaatc cttctatatc aacttcttta gttttagcac
    10921 cttgtaaagt atatcctgaa cctgggtttt cttggtgacc tgtcattcca gttattctat
    10981 tatctaatat aaccgaaata gaattacctc tattgtaaac aacatctaaa agtccattta
    11041 ttcctgtatg gaagaatgtt gagtctccaa gtacacctac caatcttttt tcattatcat
    11101 ccttcatatt aagaactttt tgagctccat gtgcagttcc aaaagcagaa cccatacaaa
    11161 caacataatc tattccatta tatggaggag caaatccaag agtataacaa cctatatctc
    11221 caccaactat taaatttttt ctctttccaa gttcatagaa gaatcctcta tgtggacatc
    11281 ctgcacagaa acttggtggt cttggtgtta ctaattcaga tttgtattct atagtatcat
    11341 ttgtttttcc aaatatagct tttcttaaaa catctggtgt catttctcca tatggtggta
    11401 ttacatcttt tccaatacaa tctattccat atgcttttat ttgttcttct ataaatgggt
    11461 cattttcttc tataacatat attttatcaa ctttttctgc aaattctttt attttttcat
    11521 aaggtaatgg atttgtaaat cctagtttca tatatgatgc attttttcca aaaacttctt
    11581 tggcaaaatt acaacacata ccagatgcta taacacctat ttttgtatca tttatttcat
    11641 agtagtttaa atcagtttca tttgagaatt ttttaagaat ttccattctt tcttcaacaa
    11701 cgccccttct tatttgagca tttgctggaa cagttaccat tttcttagca tttttaacat
    11761 actctttaat ttcaacttct tctctatcat agcactccac taatcctttt gaatgacaaa
    11821 gtcttgttgt aactctatat aaaactggtg tatcgtattt ttcactaact tcaaaagctt
    11881 cttttatcat atctttagct tcttgactag ttgatggttc aaataaaggt atctttgcaa
    11941 attttgcata catcctattg tcttgctcat tttgagatga atgcattccc ggctcatctg
    12001 ctgtaataag taccatacca ccatttaccc ctgtatatgc ataagtaaat attggatcag
    12061 ctgcaacatt caaaccaaca tgtttcatag aagccattgt tcttgctcct gcaattgaac
    12121 cccctatagc agcctcaagt gctacctttt cgtttggagc ccactctgca actattgcat
    12181 ctttatatgt tgctatattt tctaatattt ctgtacttgg agtccctgga tatgcagatg
    12241 catatttaac accagcctca taggctccac gtgcaattgc ttcattacct gtcatcaatt
    12301 gtttcatata aaattccctc catctacaat tattaaattc atagtccacc caatttgttc
    12361 aatgaaatta attatttatt tttacttcta aactttcaag tgcttctttt ataatacttg
    12421 gtgctttctt acatttatct tcagaaactg cacaaactgc aaatcttaag cccattttta
    12481 atggaactgc aaataaatta tgtttagtca attcttcaca tacttctttt ggattatcac
    12541 atggtatact tacaaagaaa cctgctatat atggaagaat ttctaatcct actctttcag
    12601 cttcttttac aaatacatct gctcttctag taagcatgtt tttgtatatt tctttttcat
    12661 cctcatattc tttgaatttt ttagggtcat ttacaatgtt agataatact gccatagcac
    12721 ttctattaca atttgaccaa tttgctctac atgaatgaac acatgagtaa tggaactctt
    12781 cagctacatc ttcacttgaa gatatacaaa ttatagctcc cattctcata ccataagctg
    12841 taaatccttt tgacatactg aatccaacca atacgagaac gttttcaggc aaattagaaa
    12901 atttcttaaa aaactttcta catacatcat catcacctgc aaagtcaata tatgctgaat
    12961 ctacgaaaaa tattatcttt ttgtctttat cttttgcgac ttcttttgat aagtctaata
    13021 ttttatccca ctcatcatct gcaacactat atccagttgg attatgcgct ggtgtgttta
    13081 taattgtaaa gactctacct tgtttttctg ctatatttac aaatttttct ttaaacgact
    13141 caaaattaaa ccttctattt tcatcaaaaa gttggtaatt aactactttt ctattagcct
    13201 cttcagctat acttacatat ggactccaga accaatcaga tgttaatacc tcatctcctt
    13261 cattagtata gttccataca gctaatttta ttgcaccact tcctcctggt gaagctaata
    13321 ctcttatatg tccttctggt aagtaatctc tgaaaaatac ttttttaact gcttctaagt
    13381 aatctggttg accttcaaga gctgcatatg cacctatctc acagttatct aatgctttat
    13441 attcttcata tacagttttc attgttatca attttcctga atcatccatt agagcgccta
    13501 ttgttgcatt tattacatta tccataccaa aagccttctc tgctgcttga gctctgccag
    13561 atatgctaaa tataacatca ttttcttttg gccacattgc atgcttacca accatacttt
    13621 gactcatacc atcaccccta aaattgtatt tactcttaca ttatatatta agcaattact
    13681 atgccaataa atcccacttt atgtaaaatt gattttgttt agaattatta tttttttcat
    13741 tattaaatta agtgttttaa ctatttttct tataaatata tatgcctgtc taaaaaaata
    13801 aaaatttgca aaaaagtaat gttaattttt cttaacactt gttttaaaaa attaacatta
    13861 cttcaaaata atttatatgt aattatattt aaatattttt attgtatttc ttccataaag
    13921 atgtcctgct tattccaaga acttgtgcaa cttcattttt agaaagtccc tgagcaaaaa
    13981 gcatacttat tattttttct tcaacatact tatttacttc ttttatatct atacttatct
    14041 taccatcatt tatcttactt atatttatag attcatcttg gacttctaca cttgctgtcg
    14101 tttcagaatt aaatttgtct acttcagagt taggaagtaa ttttttagtt ttaaaatctc
    14161 tatcttgtct caatttaatt ttacctgtca caacatattt ttgagctatt gtctttaact
    14221 ctcttacatt tccaggccag ctataattat atagcttatc catttcatct ttagtcaaaa
    14281 caaaattttc atctatactg tttattccat tgagaccatc atcctttaat acttcattta
    14341 caaaattgtt aaacaaaggt attatatctt ctcttctgtc tcttaaagga ggtatattta
    14401 tctctaaact actcaatcta taaaataagt ctgctctaaa tgtacccata actatttttt
    14461 cggttaaaga ttcattcgtt gctgctataa tccttatatc cagaggtatt atataatcag
    14521 aaccaattct cattatttga cgttcttcta ttactcttaa tagttttgtt tgaatattaa
    14581 aggatagact atttatttca tctaaaaaaa gagtccctcc atgagcaagt tcaaatagtc
    14641 ctctttttcc accctttcta gcaccagtaa atgcaccttc ttcatagcca aaaagttcac
    14701 tttcaagtaa attttctgct atagtagcac aatttatagc aacaaaaggc ctatcttttc
    14761 ttttacttat gttgtgtata ctatgagcta taatttcttt accagaccca ctttctccat
    14821 aaagtaaagt tgtataatca ctttttccaa tttttttagc ttcttcaata aattctttag
    14881 ttaatttatc tttaaataaa aaatcatcaa aagtatatct agcatagaga cctttttgat
    14941 tcaagtcaaa tcttattttt ctttctaagt tttgtagctt ggttatatct tgtattattc
    15001 ctaaaactcc ataagtacta ttatcaactt ttatcaatgt tgtcctagta ttaacaatta
    15061 gattatttat atttcttatc tttctatctt ctacgtcctc tttttcatgt aaacaatcta
    15121 acatccactc cattttagga aatacatcta agatgtattt attaagtgca cattttctct
    15181 ctactttaag catgtttttt gcactttcat tgtacaaaat tatactgcca ttactatcta
    15241 tagcaattac tccatctttt ataccatcaa gtatatttcg taacacttca ttattaaact
    15301 tttcttcacc taatgtatct aataattttt tacaatattc tacagcttct cttatagatt
    15361 catcactagc agttgcaaat acactatcaa ttccatattg ccttgcaaaa gaacatgcca
    15421 aacctcctcc tacgattaca acttcatctt tttggtctat atattttaca actttacttc
    15481 ttatctcata cttagattca aaccattctt cagttatttc agtacttatg acatttctcc
    15541 agccaacata gtcaaaagaa acttcattgt ctccaagaat caatactact tttttagaat
    15601 atttctttgc tatttctatt gctcttaaaa ggtcagttga cttcatattc attggtataa
    15661 caggtacatt tactgtatct attactaagc tataacctcc acctctacca attatagctt
    15721 gggcaccttt ttcaacaagt atacgacctt gattttccat taagtctagg ttaagtatgt
    15781 cgattattat agttccattt tctacatctt ctctatataa ttcttctatt ctttctttta
    15841 attcaatatc agaggctata attcctattt tttttcgcaa caaaatcacc tcaattttaa
    15901 attataatgt attgatttct acctgctttt tttgatttat ataggttctt atctgcatct
    15961 tcaattaata tttcaacatc ttttctacta cgacaccttt ttatacttac tccagcactt
    16021 atagttatat agttttttat tttagaatat atatgttcaa taccctcaaa ttcaagaagt
    16081 ttgaaaactc ttttaatata taattctata ttatcaatct cttcaacacc gattgattct
    16141 atagaatcta ctaatattaa aatttcttct cccccatatc tgattatgtg ttcggtaata
    16201 tctataaata cacttcttat aatatcagtt accttaacta taactttatc ccctgctaga
    16261 tgaccataat tatcattata acttttaaaa caatctatat cgaccagtat tgcgaccata
    16321 tttttgtctc tggttccatt aaataaatcc aactgctgtt ccaaatatct tctattatat
    16381 agtttagtaa gtccatcttt taaaatatca ttttctaatt tcttattatc atccactagt
    16441 aatcttaaac tctcttgttt ttgtctgagt tcatattgtt tgtgaattgc ttgagatgtt
    16501 ttataggaat tatttttttc ccacctatca gaataccaat ccatactttt ataatattct
    16561 attgccagtt taaaatcttt ttttagctca tatccatttg ctattagccc atataaatat
    16621 agatattttt ctaagattca atgaactctt tttaactaaa tttaaacctt ttatataaca
    16681 acttatacct tcatctatat taccaaattt tatatataaa tctccataca acttatatat
    16741 gtaatctagt ttagaattat atataaaaac atctccaaaa cttttatata tattttttga
    16801 taaaattaaa taggtgttta ccttttcaaa actacaatta tctttcttta actcaatttc
    16861 agctgaatat aaaaatataa atgaatataa ataatcctta tatacacctt caacaacttt
    16921 cattaatttt atagtcttta ataaaataac ttctgcttct ttattcatat cttcttttaa
    16981 atatgcccat ataaggtcta tttttataga cactctttca gttactgata ttgagttatt
    17041 actgtgtgca acatctaata cattatttat gtattttata gcttctacat tatttccact
    17101 ctctaaataa cacaaactta ttatacattt acaaaaagcc tctatagagt ataaattatt
    17161 ttcttgagcc aattttagac attcaattaa gtattccata ctttttataa agttattaaa
    17221 taaatataga taaactctcc caaatatata atttgcccta cttacaagag ctttattttt
    17281 taaagtttta aaaaaagaca cttcttctac taaagctatt acatccgttt tggtctgata
    17341 taaagcactt atttccacac ataaactcat atttaacaat accaacaact catttaaatg
    17401 attttgcctt ataatttttt ttgctgagaa ataaaaatta tttaccttat catattttct
    17461 tatagaaata gctgataaag taagataaat ataaatttta gatattatat ctgaatcatc
    17521 ttttaatgac aattttttaa tatactttaa tgactctatc aaatagttgt atgatgcttc
    17581 tctatctttt tttatatagt ttataagact aattaaatat aaagtataga ctctactatt
    17641 atcttctaat tttgtattta aaaggtcttt gtgataatcc aagatgtagt ttaaatctgt
    17701 caaatttaga tagaatttag tcaacatatc gtattctatc ttgtttatat ttctctttaa
    17761 atcttctatt aaactccttt tcaagtaaat caccaatcct atttcaacag ttaaaaattc
    17821 ttaaaatcat gataatgcaa acatctattt ctaccacttt cctttgcttt atataaagct
    17881 ttatcagcat tttcaattat ttcttttata tctatactac tatttttaca tgtatcaatg
    17941 ccaatactaa ctgtcaaatt attatttaaa aatttcttta caatcctaca taattttctt
    18001 ccaaatattt tacttttttt ataatcttta catacagata atatcagaaa ttcttctcca
    18061 ccatacctta tcataagcat gtcttttttt agatattttt ttattattat agttatattt
    18121 ttaagagcaa tatctccatt ataatgtcca taagtatcat tgtattcttt aaagtaatct
    18181 acatctatca taaatgctgt gcaatattca ctaacttctt gtttttctaa aaaatcgttc
    18241 aaaaaagttc tattatatgc acttgtcaaa tggtcaacat actttttttt atctaaatca
    18301 agttttactt ttctcatgtt atttatttct ttttctatat actcatgtct ataatttaag
    18361 gaatctatat aatttctatt tcgagctttt atatacatca tttttaactc ataatacatt
    18421 ttaaagtatt tcatactcat ttcataattt tgaatctttt caaaacacaa atatatactc
    18481 ttgtatgcta acttcacata attataattt ttagcatttt tagatttttc aaggagtttt
    18541 ttatttgtat ttaaggcatt gtcataatat ccaaagatat aatatatctt tgcctggtga
    18601 taaattattt ctaaatcata tattatatat tcttctatat tttttatcat atttaatatg
    18661 tttttagctc tatctaaata gcatattgat tgttcaaaag atttttcatt actttttgca
    18721 taatacatgg ctaaacttat aaataaatca aacttctcta cttcttttaa gctatgattt
    18781 ttaatttttt ctagatatac ttcagatttt gctaaattat ttattgcgac aggataattt
    18841 ctagtcttta ttaatatttt agttatgttg tttgatgctc tatatttttg aattatattt
    18901 acatttctat actttttatt gcttattact tcattgaaat attccatgga acattcataa
    18961 ttttcaaacg aacattcaca ctcacctatc tttatattac atagtgaaga tacttgcata
    19021 tcctttagta aatctgcata ttttcttgct tttaaaaaat ttaccttagc aattagagca
    19081 tcatccaaaa acaaaaaata tattttacca ataataaaaa aatatatttt tttacaatta
    19141 aaatcgttaa tttcttttat ctttatatac aacgtttcta tttctttaat tattacagaa
    19201 gattctatac acttacttgc cttaatgtaa ataagttgtg atttaaaaat aatttcttca
    19261 attagaaaat tattttcatc acaaataaga ttaaatttat ccatatataa atttgcctta
    19321 tttacatctt cattttttaa acttaaaata gctatttgtg aataaaatcg tgctaaaaat
    19381 ttataatctt tgggtaagca tttatcaata tatttacagg aaatcaaaat atactctttc
    19441 attttttcac taaacccttg aataccatat aataaagcta gcaaaaaatt tgattttaat
    19501 aacattaaac tattttgttc agttttatag caatcaatta aattctttac tacatcaatt
    19561 ttattttcaa taaaatcgtt attagtcaat aatctttcat tcaaggttag tttaaacatt
    19621 gaatctaaaa ttgtatcatc tatctcttcc aaatatttac tatatattgt aactcacctc
    19681 cgacttacat tatctcataa atttagtaat tttccatact atttttgaaa aaagttgatg
    19741 tctcaattta gaaatttctc ttaaattaag acatacttta cactaaacta tacaattttc
    19801 tatctgaact atataatcct gacaattttc gcaaattatc acacttacaa tataactatt
    19861 atcaacttta tttgcccttg atagtttatt aaaagatagt cctttataat caacagatat
    19921 attgcttatt acatattctc catctttact gaaaatataa aagtgatatt ctaaaaaagg
    19981 aaagaatttt ttcttttctt ttactccttc taaacataat ggacatgctt cttgataata
    20041 ttctacatca aaatcaactt cttctgcttc attaagtata tcaattattt ttgaaatact
    20101 tactccttga actggatttt cttcatcatt tattaaagca ctacagttct cctcttttaa
    20161 taaatattcc ttatcatcat atataaactt atataaactc atattatatc ctccttataa
    20221 ttattaatct ccaagttatt ctaccattta aataaagtta ataaaagtaa ttgtttataa
    20281 tctttataat taagttatgt atacatatat tcctatgcgc tatcttaatt aatgatttta
    20341 aaatccaagt ttgcactttg aacatccagt ttcccaaaat ctattgtcaa aactcacttt
    20401 agttattaat ataatgatat aaatatataa ctgcatagat gttacacact taactattta
    20461 atatgatacg atttggtatc taaatttata tattgtatat agggggagat atatatgtat
    20521 aaagataaaa gtgatgaatg tatacactta atgactgcat acatcgatag tataagtgga
    20581 tattattcat ttattgacac acaattagag gattttatga tgaaatatgg tgaaaacatt
    20641 gttgattcaa atttacacag tataatgatg ttgctttgta aatggggatt atcttagcat
    20701 ttcaagaaac ttttttgtat attgtattct atattattta ttcattaaca aaaaagattg
    20761 acataaactt aaaagctata tatagatttc tttaaaaatc tatatatagc ttttttaatt
    20821 ttttcaaaat gtatacttaa ataagttagt ttttttcaaa atttgactat ctaatatgat
    20881 ataatttaac tatctacaac aataaaaaat atctaatatg tattatctag tttagctcat
    20941 cataataaca tccctatatt tacaattaag atattttcaa tatagtaata ctcaatttta
    21001 atattataaa aaggagagat tttgtatgga ttacaatgac ttactaaaat cagctagaga
    21061 gaactttaat ggaacatgta aagtatgtaa aatttgtaat ggattagctt gtgctggaga
    21121 tgttcctggt atgggtggta aaggaagtgg ttcgtctttc attgaaaata gaaaaagtct
    21181 agaaaaaata aagataaata tgagagtcat acataatgtt tcaaagccag atacatccat
    21241 agaattattt ggtagaaaaa tgagttctcc tatattcgct gcacctgtat caggtactat
    21301 attgaacatg ggtggtaaag tttccgaaaa agaatacata gagcctgttg ttagaggttg
    21361 ttctaattct ggaatttatg ctatggttgg cgatactaat gtagatactt ttttactgga
    21421 taatttagat gtattgaagg ataatagggg aaatggaatt gtatttataa agccttggaa
    21481 taattcaaag ataatagata aaattagatt gtctgaagaa gcaggtgctt ttgcagttgg
    21541 tgtagacctt gatgcttgtg gacttattaa taatcaattt caagaaaatc ctttttctcc
    21601 taaaacaatt gatgagatta gagaattggt tgaatccact agattacctt ttattataaa
    21661 aggaattatg actgtagatg atgctttgat ggctgtagag tctggtgcta gtgccattat
    21721 tgtttcaaat catggtggaa gagtacttga ttatactcct ggtacttgtg aggtacttcc
    21781 agatatagct aagtctgtaa agggaaagat aacaatactt gttgatggtg gagttaggac
    21841 tggtgttgat gtagttaaga tgcttggatt gggtgctgat gctgttttga tgggaagacc
    21901 ttttgttact gcttcttttg gcggtggtct tgatggtgtg gagtttttta tagagaaggt
    21961 taggaatgag ttatgtgaga ctatgatttt gactggttgt caaaatgtta aggatataga
    22021 tggtagagtt atatggaatt tattgtaaaa tagttttata acatttatat ttaaatagtg
    22081 tctcatttaa attgaaaaat catattgaga cactatttaa tatattttta gattttaatc
    22141 ctatgaaaga gtagggtcag ttagtctacc caatttatcc caagtagctg gaccaattat
    22201 tccatctgca gacaaaccgt attttttttg gaaacttaca actgcattat atgtcttact
    22261 tccaaaagaa ctatctatac ctacattata accaagtaaa gttaacatga tttgtgcagc
    22321 ttttacttct tttccgctat taccctttcg tataactttt gttgtactta tagtcaaata
    22381 ctcattactc ataattgtta tagttttagc acctatatta taagaaacag tataacttgc
    22441 ataaacacct ttactattgt cctcattcat tagattagct gattctggaa caaatatctc
    22501 atcatctggc ttagggtcta tatttatctc ttcacaattt tgtttatgtt catctcttat
    22561 ttcttgtggc gcattttcat atgtatatat atacccagaa agagtaaaag aatctaggtt
    22621 tttctctgga acagtctctt tacttcctcc tttctcttta cttgtgtcag caaatgccac
    22681 acttgtaaaa gtaagcaata agcaacacat aatagctact atttttctta attttgttaa
    22741 cataaaatcc tccttataat tattttatta attgattata atctttcata tccatttcta
    22801 ttatgtcata gtctctaggt attttacctt ctaattcgca tttatcttca taaaatttcc
    22861 taatttcatt tggtgcatct ttataataaa tatttataat tttatcagtc gtcttattca
    22921 ataaaaattc attaatattg ttattcattc cattattacc ctcatcaatt ctaacttgag
    22981 catcaacttc aacaaaagat gttatagaag tcataaatac taaaacagca attagtattc
    23041 cacttttgcc tttcttttta acaatactct caagtctctt ttccaaattt gtcttatcaa
    23101 actcggttgt caaaatctta ctattataat ttttactaaa cttaattgac tttataagtg
    23161 ctagtgcata ttctttttta tcttttaaag aacattctac caaaacactt tcatcacaac
    23221 ataattcaca atctagattt actttattgc tcattacata tacaagtgga ttaaaccaat
    23281 atattattct aacagacaaa actaaaaact ttattaaaat atctttattt ttaaaatgca
    23341 ttaattcatg tttcaaaatc caagttagtt catctttact ataaggatag tctggtaaga
    23401 atatgtaaga tttaaataat cccatacctg ctggacttat caattcatca gaacctctta
    23461 attcaatttt cttttttata tttaattctt ttaacaagtt tttgtagata cagtttatat
    23521 cattatcttc aatatcataa gatacatcaa caactagatt tttaaatctt gtatacttta
    23581 taaatgtata aactgcaatg actatagtta caaataacca taaataagct acatacagca
    23641 taagattatt atctgtagaa atatcaattt gtgtaatatt atttcctaat gcattttctt
    23701 tgggttcttt tacttcatac actattgtat aattaaatag aaaaagtaac attctaaaga
    23761 ctattatcaa ccaaatataa taattgaatc tcttactaaa cttcttaaat aaatatcttt
    23821 tcaaaaataa taatagacaa ataccaagac tacatacaat agttgtcttt ataagatttt
    23881 caaaaacata tagcatataa ttattcatcc tttaaattat caaaataact ttctagaaaa
    23941 tctagcttat ctttacctat attttcatca tcatgtaatg ctgaaattaa gcttcttaaa
    24001 gagtcatcat gtatattact taggaaatcc tttgtctcaa aacttaaata atctttgtgt
    24061 tttactctta ttgtgtaatg tgtatatctg tctatttttt gagcatctaa gaatcctctt
    24121 tttaccagcc tagataaaag tgtcagtgtg gtggtttgtt tccagccata tttttgttcc
    24181 atagcttcaa taacatcttt tgatgtcact gtaacatcta ctttccaaat gaatttcatt
    24241 acttttagtt ctgcttgtgg tatttttctt atcattattt acacctccct caaaactcct
    24301 gctaccttaa ttctacaaca tgtgaatttt ttagtcaaac actttatgaa aaaaatgtga
    24361 atttttgtac tttttgttaa atttctaatt tttttacaat attaaaagtt agattttaaa
    24421 atatatctat ttaaatattt tacttttta
//
LOCUS       DAMJZE010000039        23882 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJZE010000039 DAMJZE010000000
VERSION     DAMJZE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.039"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..243)
                     /locus_tag="PC541_001455"
     CDS             complement(<1..243)
                     /locus_tag="PC541_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDF2838923.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAIN"
     gene            complement(221..913)
                     /locus_tag="PC541_001456"
     CDS             complement(221..913)
                     /locus_tag="PC541_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838924.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(983..1492)
                     /locus_tag="PC541_001457"
     CDS             complement(983..1492)
                     /locus_tag="PC541_001457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838925.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(1513..2487)
                     /locus_tag="PC541_001458"
     CDS             complement(1513..2487)
                     /locus_tag="PC541_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838926.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(2790..3026)
                     /locus_tag="PC541_001459"
     CDS             complement(2790..3026)
                     /locus_tag="PC541_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838927.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            complement(3511..3990)
                     /locus_tag="PC541_001460"
     CDS             complement(3511..3990)
                     /locus_tag="PC541_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HDF2838928.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            complement(3983..4120)
                     /locus_tag="PC541_001461"
     CDS             complement(3983..4120)
                     /locus_tag="PC541_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838929.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            complement(4144..5514)
                     /locus_tag="PC541_001462"
     CDS             complement(4144..5514)
                     /locus_tag="PC541_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2838930.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(5463..5789)
                     /locus_tag="PC541_001463"
     CDS             complement(5463..5789)
                     /locus_tag="PC541_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDF2838931.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(6071..8488)
                     /locus_tag="PC541_001464"
     CDS             complement(6071..8488)
                     /locus_tag="PC541_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838932.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(8500..10458)
                     /locus_tag="PC541_001465"
     CDS             complement(8500..10458)
                     /locus_tag="PC541_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDF2838933.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(10458..11030)
                     /locus_tag="PC541_001466"
     CDS             complement(10458..11030)
                     /locus_tag="PC541_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDF2838934.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(11053..11625)
                     /locus_tag="PC541_001467"
     CDS             complement(11053..11625)
                     /locus_tag="PC541_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF2838935.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(11678..12013)
                     /locus_tag="PC541_001468"
     CDS             complement(11678..12013)
                     /locus_tag="PC541_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838936.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(12028..13194)
                     /locus_tag="PC541_001469"
     CDS             complement(12028..13194)
                     /locus_tag="PC541_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDF2838937.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(13206..13643)
                     /locus_tag="PC541_001470"
     CDS             complement(13206..13643)
                     /locus_tag="PC541_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838938.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(13714..13863)
                     /locus_tag="PC541_001471"
     CDS             complement(13714..13863)
                     /locus_tag="PC541_001471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838939.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(13844..14236)
                     /locus_tag="PC541_001472"
     CDS             complement(13844..14236)
                     /locus_tag="PC541_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2838940.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(14267..14464)
                     /locus_tag="PC541_001473"
     CDS             complement(14267..14464)
                     /locus_tag="PC541_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838941.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            14540..14914
                     /locus_tag="PC541_001474"
     CDS             14540..14914
                     /locus_tag="PC541_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838942.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(14924..15133)
                     /locus_tag="PC541_001475"
     CDS             complement(14924..15133)
                     /locus_tag="PC541_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838943.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMSKSEKRKISEITKERIRK"
     gene            complement(15180..15995)
                     /locus_tag="PC541_001476"
     CDS             complement(15180..15995)
                     /locus_tag="PC541_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDF2838944.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG"
     gene            complement(15998..16219)
                     /locus_tag="PC541_001477"
     CDS             complement(15998..16219)
                     /locus_tag="PC541_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838945.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            16372..16935
                     /locus_tag="PC541_001478"
     CDS             16372..16935
                     /locus_tag="PC541_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2838946.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            16959..18548
                     /locus_tag="PC541_001479"
     CDS             16959..18548
                     /locus_tag="PC541_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2838947.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            18811..19317
                     /locus_tag="PC541_001480"
     CDS             18811..19317
                     /locus_tag="PC541_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2838948.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            19557..20687
                     /locus_tag="PC541_001481"
     CDS             19557..20687
                     /locus_tag="PC541_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2838949.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITVEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(21048..22736)
                     /gene="ilvB"
                     /locus_tag="PC541_001482"
     CDS             complement(21048..22736)
                     /gene="ilvB"
                     /locus_tag="PC541_001482"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2838950.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(22789..23784)
                     /gene="ilvC"
                     /locus_tag="PC541_001483"
     CDS             complement(22789..23784)
                     /gene="ilvC"
                     /locus_tag="PC541_001483"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2838951.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
BASE COUNT         7576 a         4331 c         2549 g         9426 t
ORIGIN      
        1 atttatagct cctgtaagct ctgcatacgt tgaatactta tgtgtagctt ctgactttct
       61 aactactaat agattagctc cttgatactt cttatctcct aactttagta tatagtcttg
      121 tgctacatta acagattttc cactccctgc tgaacctttc attgctctgt atcttttttt
      181 agtaaaatta gcttccttga aatctggatt aaaatttact ctaactatca tttctatcac
      241 cataatctac acttattttc aactcatcat ctccaatatc atctttactt aggttatcaa
      301 cttcacattt caacttctca actcttgttt tctgctcctc tgtagccaaa ttccaatcct
      361 tatgaatcat ttcatcatac tgtttaatta aacttctaag ttcactcata gctctactct
      421 gtgcattaag aaaagatgct tgcctatccc atgcaaattg aaattcatac tctatctttt
      481 caccattttc tgtgctttca tatttcttta attctttaac catttcttcc ttgcctttaa
      541 catacattat cttttgtgct cttattattg ctgcatattg aattgttatc tgttcccaaa
      601 gaatatcaaa tttatctttt atagatattt cttgtatcaa ttctctagtt tctttgggta
      661 gatactttga gaagaaacca aacttttcag cattcttatt ctcttttgga gcaccatgac
      721 caactgaatt tttattagaa aagggtgcac ctcttttatt tataggtgca cccttctttt
      781 tttcactagc ccagttgtat ctttttatcc atgactttaa agtgtttaaa ctaatgtcat
      841 actttgatga tatttccttt tgtttcattc cttttaagta atcttgtttt accttttctt
      901 tgacatcttg cacatcacca cctctttatt tgtttgtttt gggaataaaa aaacaatcct
      961 atttaatagg attgtttcaa atctagtctc ctctatcctt ccaggataaa tgaaacatac
     1021 ttattattaa tgtagttaga gataaaatta tatgaactgt aaaaaatatt aaaacataga
     1081 aaattatatt agaataatat cgagtaagca ttattgtatt gataaaacat attacaacaa
     1141 ccaaaatctc cattattaca gtataatatg cttttaactg tatgaattga aagtatgata
     1201 tattaaatcc atttaccttt ctttctgttt ttatatcttt tgcttctgat attgatttgt
     1261 tatatgtagt aaacaataat gtaataatgc aaactccaaa agctactaat agtcctgaca
     1321 aagttagtac atctttgttt aaagaaatta caacttcttt attaattgac gaattaaaaa
     1381 acatgtatga aataatacat attaatactg gtgttaaaac taactctact acttctttag
     1441 aatttatagc cttgtaataa ttgagtaaga tatttaatac ctttttatac attttaacac
     1501 ctccttcgca atctatgctt cactcatagc aacttcttga tacattaaat taaaaaaaac
     1561 tgaattattc tcaattaaat catctatcaa attattcatc ttatcaaaaa tatcttcact
     1621 atctatatga ttatctatat caagagaaac ctctatttct ctacacaact ttataccctc
     1681 agtatccaac tttaattttc tcttgttttt ctctccctca attattatcc tatttatttt
     1741 tttatttcta aattcctctc ctttaatata tttttcgcaa catcttttaa cttcactagg
     1801 taaatatcta gttctaaatg tgggcttata tatcaaatcg ttatgtcgtc ttgaattatt
     1861 taaattagaa aacaaattat cttcatcagt tccaaacttc tctctatcaa catttaaagt
     1921 tatttttgaa acaccatcta aatctaatat ttgttctata aaatctttac ttgctatttg
     1981 tgtaatatta attgatgttt ttgacatatt attatcaaat ttattatttt caatcatatc
     2041 ctcataatat agatgtaagt ttttacttag ttcaatcatg ggaaccccca caaaaactct
     2101 ttcaaataat actactgcta tattattttc cttcataaat ttaataaata ggtgctgctt
     2161 attgctatct ccttgatcca tttttttatt accctctact tctttagtat gagcatttat
     2221 aatatttgta ttctgattgt atttagtata ttcaagtttc accttgatta ttaaatcatc
     2281 ctcgctacat tttacttctg taatccaaaa ctttttatct ttagtaatat taattccatt
     2341 tttgttttct ttacaatatt ctttatcctt aaagaaggtc tttattacct cttttaaaat
     2401 ttcttcatct gaatagacac tcccattaat tttatttata atatctattc tataaacatc
     2461 aatatatctt tttttaaaat tactcattat accattattc ctttcacaaa tatttataat
     2521 gatataattc tatataatca gtcaatatcc ttcaataatc attcgacaac cacaaaataa
     2581 gtctaaataa tacttttacc ttattcctgt ctataatgta caaatatatt tcatttttaa
     2641 ttcattacat aaaaaaaaga ccctccatta atacagtcat ttaaatcatt tctattaatt
     2701 ctttaatctt ttatatacct ctttataatt catactttta tctatcaacc taggtgattt
     2761 catagatata actctttcaa gtgcttgtat taataaacaa actcttgtat ttcatctgta
     2821 aaatcttttc tgaccttttt accttctgtt cttatatcca gccattcttg atctttttct
     2881 gtggcaatat agtaaccatg tattctaatt tgtttaagtg cttttgtaac ccacttcgta
     2941 aatagttttg cttctgcttt attacttcta aatgacatat tgtacacagc ttcttctgta
     3001 acaaaagtag taccaaagtt aggcaattta tctttaaagt ttctagtgta ggaatctccg
     3061 acagtagact cattaatttc tttttatatt ctctatctat atttcttaat gtatcacgaa
     3121 tatttactat gcctagttcc tctcctacgt catttgcatt aaatcaaact tcttctccat
     3181 tttttgacca tatcatcctt acatttttct cttgtaaaat tttcaacata ctactacctc
     3241 ctaaattagt tattaataaa gaatattcaa tttgaacacc cttattttcg acccttcaat
     3301 atgagggatt aaaattgtat aaaaaagacc tagaaatcac tctaagtcat gaattactca
     3361 tttatcattc tccttattta caaaataaaa aagactaagt tggggtaact tagccttttt
     3421 caagggggaa tatattatac acttgtttca tactaccatt ataacagggt taaaatatca
     3481 ttaaaatatc atctttttat catatattta tcaaacagtt aatttcaagc catctatacc
     3541 gaataaatac actcccagtt cctttatcat ttcattaatc caacgtcttg atgtaactac
     3601 tccacaattt aatatctctg ctatttcttc atatgttttt tcatcacaat agtaaagctc
     3661 taaagctcta tacttttcat aagaatgtaa acgctcttgt cttaatttca aagttttcat
     3721 tgctgaatct atatgtgcta tcataataat agttcttgct ttacttttct ttatacttaa
     3781 aatatacaac tcttcattat caagcttacc taaatcatcc tctaagtaat ctacatcttt
     3841 agcttcactt accgctgagt ctatatgtct cttaaaatca ttataatgct tcatcaataa
     3901 tctagtatta tataaaacgc cttttttcct atcttctctt tcttcttttc ttaactctct
     3961 tacaatttct tttatacttt ctttatccac ttaaatcact ccttcttgct ctcttcgaac
     4021 aacttctact gcaatactta actttagatt tatccaactt ataaaatctt ctaccacacc
     4081 aagcacatct tactatttta cctgggctta taagttccat cttttctttt tactttcata
     4141 ccttcactcc ctatattctt ttaattttgc tttgactgct tcaagtaatg cactttgtcc
     4201 tttatcttta ttctctagag cctccattac ttgttcatct attgtacctt tacagattag
     4261 atgatggata ataacagttt ccctttgccc ctgtctataa agccttgcat tggcttgttg
     4321 atacagttct aagctccaag ttaatccaaa ccaaacaatt atacttccac ctgattgtaa
     4381 attaagtcca tgtcctgtac ttgctggatg acaaagtaat aattgtattc ttccattatt
     4441 ccaatcatat atatcttttg agttttctat tgttcttggt ttcaaactct taaactcttt
     4501 cattaaacga ttataatcat gtttatagtt ataaaaaact ataataggct taccatttga
     4561 aacatctata atttctttta aagcttctaa tttttctcta tggagttcct ttacattttt
     4621 atcattatca taaattgctc cattagctaa ttgtaaaagt ttatttgctg ctacagctgc
     4681 tgatgaagct gttattatat ctttttctaa ttctaatatc ttctcttttt ctaattcttt
     4741 gtaatattta agtattttac tatcaaggtg tatttcaatt ttattatcta tttttttagg
     4801 catattcaaa taatcttctg cttttaagct tatacaaata tcttttattt tattatgtat
     4861 tgcattttct gctccatctt taggctgcca attatatata gctcctgttt gataattttt
     4921 ttgtcctggc tcaaagtatc gttctttata tcctgtaata gttttaccta gtctttcacc
     4981 tctatcaagt aagtacatct gtgcccataa gtctattaaa ctatttggtg ttggtgtacc
     5041 agtaagccca actactcttt tagttaaagg caatactttc tttaaacttt taaatcgttg
     5101 tgctttattt gatttaaaag aactaagttc atctatcaca accatatcaa atggccattt
     5161 tcttttataa aaatccacta tccagggtac catttctcta tttattatat aaatatctga
     5221 gtctgtactt aaagctctca ctctatctaa tttactacca agaactttag atacttttag
     5281 atgctttaga tgctcccatt tttttacttc actactccag gtatctcttg caactcttaa
     5341 aggtgctata actaaaactt ttgatatatc aaagtaatca tacattaatt cacttatagc
     5401 tgttagagca cagactgttt tacctaatcc catatctaat agtaaaccta tattattatt
     5461 atctatagtt tttctaattg tgtattcttg gtatggatgt ggtttaaatt ccatccctta
     5521 acctccttga taaaatcatc tatctctttt aatgtgctta cacacttaac tttaaatcct
     5581 aattctctta attctcgcat tttatattgt tgaagctttc ttagtttttt acctggtgct
     5641 ttaagttcta caaatataac atgtccttct ggtaataaga caatcctatc tggcacacct
     5701 gcctcacctg gtgaaataaa cttcatagcc tttccaccta acaactcaat ctctttttta
     5761 agtctttttt ctatttttga ttctaacaaa atatcacttc cttaaaattt aagtgtcaac
     5821 aatgtcaaca gattttctat atatatgtat atatacatat taggcataca tatgtatata
     5881 cgtatatgcc taatattact tatactactt tatacttaat ttttgttgac attgttgaca
     5941 ttaatacctc aaacattgta attactgcat cttaggtgtc aacataagcg tcaacaaagc
     6001 catttttttt gttgacattg ttgacacttt taaaattttc aaatttactc tatttttaat
     6061 tctgttgaca ctaactttta cgtacataag ctctctgcct accatatatt ttcccaaacc
     6121 ttaaatttcc attatatgat atccaatcat ctaatccttt taatatatca ttaatttctc
     6181 ttgataagat aggcgtgagt tgtttaggtt ctccattaaa tagctcaacc catatctcca
     6241 taacacacgt tttttctctt aatattgtcc cttcttttaa atcaccaaaa tctgaaccat
     6301 gaatatattc tctcttttct gaaatactta aatcatacca attcttagta ataggtttat
     6361 ttagatactc ttcgataatt ccagatttag cattttcttc tgagtgagtt ctttgttgtc
     6421 tttcagcttc ttttttctcc tcatctgata gatatagctg ttcattggct ttatataatt
     6481 ctactgcttc tgcccaaatt tgatttcttt cattatcaag ttggccatta aatatactct
     6541 tgttagtctt ctttactcct gcatctattg gccaaaacct tctattccca gtcttatctc
     6601 ttaaaaattc tttatcatta gttgttccta taactacaca ttgacgtaaa aatcttgagg
     6661 ttctcttccc atatgcgacc ctatatatat cctctgattt gcttagaaaa tgcttaactg
     6721 cctcaatatc tgctttttta gtagccatca tttcacccat ttccagcaac catactcctt
     6781 gtaattgttc atacgcttct ttaccttgta cagtagttaa actatcagaa taccagtctc
     6841 caccaagttt tttaataaaa gtactttttc ccattccttg aggaccagat aaaaccatca
     6901 tattatcaaa ctttattcct ggattaaata ctcttgctac tgctgctact aaaacttttc
     6961 ttattatagt ccttgtatag tggttatctt ctgcacctaa atagtcaatt aaaagtgtat
     7021 ccactctctt aatgccatcc cactttaaag aatttaaata atcttttata ggatgaaaag
     7081 tattattttc aaaagcaatt attagagcat catttacttt tgatggtgat gagatattgt
     7141 aaattgtttc tatatgatgt ctaagcccag aatcatcact atcattccaa tcatttaatt
     7201 tatcatcttt tctccaaggt aacttaccta aaacgacagc tctatttgaa aattcattat
     7261 aagctatttt tcctttcaaa tatgggtcat tttctataaa cattaagata ttgtttgttg
     7321 ttttcttata acttccctta ttgtcataat ctaacctagt taaccattca tcatcttcaa
     7381 aatctatatc accaaaatca tccttagctt tatcaaggtt ttctcttcct atagtctttc
     7441 gtactttagt gtcactgctt gcaaattcgc tcattcgagt aaatgaaggt aatctattta
     7501 caggcgtttc gggtttagca tcttcatcaa gttcaccaaa tttatgtatc ctaactaaat
     7561 caaaagcatt gcataaaatt ccgcttgctg ggtctgtacc atgatgacta tatgaaaact
     7621 tgtcatcata aataactact ccaccacttg tactaccttc tgaatatgta tacctggttt
     7681 catcaatacc aggaatatat acttcattta agaaagtttc tatagtttct tttatactat
     7741 aggacctgca aaatgcacct ataattccag ccttttcaat agggtcttgt tgttttttta
     7801 attgtgtatt aaacttttgc ctctccctcg aactttctgg ccaatagctt acatctgtcc
     7861 agtctaaata taaatctagt atttcatttg ggtttaaaaa ctctccatct tgaaatttaa
     7921 atatatagtc tccatcaatt gaagtacttg gaaaatacat aagtctatga ggttgatatg
     7981 tagtatcatc aaacatatct attcctatag tatctgctat catcctggaa attgcttgat
     8041 attcttctgg tagtactggt ctagttaaag gtattacaag tctatatctg ggattattct
     8101 cagtatgtga gtgcgtagaa tacataagac aagcataatc atttaataat gttatatcct
     8161 cccatatatc tttattcgca taatctatat ctaaagttat aatacttcta tttgcaatat
     8221 tctctgcttt tcgtcttcca ttttttaagc ttccacctac aaatccacca acatctttaa
     8281 ctctatcttt ttcagtcttt gacatcttcc tatattctgt atatgtttct tgagttctta
     8341 atgttttact taatctattt acaagttcgg accataaaat acttttattt ttccaatgtg
     8401 tttctaattt attttttcct atggccagca tgagttggcc atcatgtctt acatttatgt
     8461 gttcaatttc actggccttt atatccataa atcaatcacc tagtctttct tataataatc
     8521 acattcatat ccatctgctt taagaggaag acctttagcc caagatattt cttttcccat
     8581 aatactgtta acttcttcta aagaacctcc ttttttatct acatcaatta caagctcatc
     8641 atgtacatgc attacaatgc tataacctgc atctgtcaca ttaaacatag cctctcttaa
     8701 gcaatctcta gctgtagctt gaacaatatt ctcaactaac ttaggtccat aagtatctat
     8761 tcttttccat tgtttacttg tctgttccat accttcatat gttatcttat ccccactaaa
     8821 tgtagtatgt ggctctatct taggccttag atacgataat cttctactac ttggtagttc
     8881 tataaataaa acgcctggat tataaataaa tttaatccca tgttgaattt ctactatagt
     8941 cctatctttt atagcttttt tagctgcctt atctacatcc caccaaaact tagttatatt
     9001 tggattagca tttctccatg cagtaacaat aggttgaagc tcttcttctt taagccccat
     9061 tttaatagcc cccatagaaa ttaaggcccc tacacttccg ccatatccat tcgataattc
     9121 tgctagcttt cctttttgtc taagttcaga acctttttta atattttcga ttggaatttt
     9181 aaacatctga ctggcactag cttcatatat ttttccatga gaattaaaca catccagtct
     9241 ccacttctca cctgcaagcc aagctataac tctagcttct attgcactaa aatctgacac
     9301 tataaatcta tgaccttcac ttggtataaa agctgtcctt atcaattgac ttaagacatc
     9361 tggtacacta tcatataaaa gctctattaa atcaaaatct ccttctttta ataggtttct
     9421 agctaggtct aaatcctcta tatgattttg tggtaaattc tgtacttgta ctaatcttcc
     9481 tgcccatcta ccagtcctat tagccccata aaactgtagt agacctctta ctctattgtc
     9541 attaccttta gctaatttca tagcctcata tttctttata gaagttttgg acattaattt
     9601 tctaagttct aaaattctaa ctacattttc atcatcaact tgttttaata tttctggaat
     9661 actttctttt gttaggcttg taatctcaaa gccaacttta tcacttaacc atttttttaa
     9721 ttgagctgga ctatttggat tatttagacc agttatttta attgcttctt ttgtaagttt
     9781 ttcagtatat cttttatcac attctattgc attctctatt aactctgtat ccactttaat
     9841 accagtatca ttaattctct ggtctaaata ccataattta atttctcttt cggtagtctt
     9901 atacttacta agtttgtttc ttatttctct ttcaactaca acatcttgtt tacaatattc
     9961 tttaaattta ttccattttt ccatatcatg tattggtaaa tttctagttc ttcccttgtt
    10021 aacttttgta gccttacaag gtttacagaa atattgtatt aatgctttac cttctttcat
    10081 tttctgttta tcttcattaa actttaaagc cttagacaca ctatctaaac ttcctggaag
    10141 tcctagtgtt aacgccttta tcattgtaca tgaccactca tttggtttta aattaatatt
    10201 taaaaattta cttattgatg ttctttcaaa attagcatta aatgctgatt ttataacttt
    10261 attatcattt aaagcttcta ttacttcttt tggtaactct tcatcattta ctaaatcaat
    10321 aacttttacc tcttcattat caaaagcata ggcaaataac agtatctcaa aattagcaga
    10381 gtctacatat ctgtagactc caactttttt tatatctaaa tcactatatg tttctatatc
    10441 aattgataag gtcctcatta acttaagaag tcctcttctt catcttcata ttcaaagtca
    10501 tctgagaaat ctgcttctgc actagctcta gctcctccaa gtacttctcc atctgctaac
    10561 ttttgaacat tttgtaagcc acaacctatt cctttatttc ctgcactatt atatgggaaa
    10621 aagtttatac taactcttcc ataacaacca ctatatacct ctgtattatc taatatttca
    10681 tttaagtctt tatcaactat tcctggcttt tgagtactat ttgcatttaa aaaatacatt
    10741 cctacatatt cctctgcttc atcagctctt tctgcatctc catcacgaag aggtgttttt
    10801 aaattacctg gtaattttcc accccattta gaagttttac cttgttcttt agctgcatca
    10861 atagccttct ttattcttcc taaagtcacc ttatctgatt ttggtattaa aatacaaact
    10921 gaatacttgg gctctgcacc ttctaccatt gctctgcttt taaagatatt gcaataactt
    10981 aatcttacct ttcctgttac tacctttgtt gattgtactg aattactcat aattttcttc
    11041 ctcctaatat ttttatattt ggtatattga ttttatattt gaagttattt ctccaaattt
    11101 atctaaatct ctaacttgct tactaaacaa tttaattctt ccacaaataa ctgttgaaga
    11161 aacatttaat aactctccta tatcagatat ctttttatct tgtaaaatcc aatctactat
    11221 tttatcaata tcttttaaat atgactttct tgcaattttt cttatataat caacatcata
    11281 gttaaattga atttcataaa atccatgtat atcttttaat ggtcttttac tatcttcaaa
    11341 tacacttaca tctaaacctt ctatagagtt tctataatgt ttttcgccat aacgcaaagc
    11401 tttaaacatt tctattctta tgtaagaaac agccactgta gaaaacttgc ctctgttact
    11461 atcataattt aatgctgctt tatataaccc aatacatcct tcttgataaa actcttcata
    11521 taagtatgga tgttgctcta cataagactt aaaaaatcta tttatactaa aatgtacaag
    11581 attcaaatta ttttcaacta gtttagttat ttcttgatta ctcataaaat tatatcccct
    11641 tttaaatctt tttatagcta ctaggaatta aatattttta attatccttt gctcttactg
    11701 atacaaagca tactggacta acttttatat gttctggtat atacactcta tcaagatgat
    11761 tgtcatttac ttcgaggaaa gttgacatca ctttatcctt tattaaactt tctcttttaa
    11821 aatccatcac tataactttt gcattttctt cagttgagtt atattctgta atatttagta
    11881 tctttaacat atctagtaaa ttatcttttt cataatctaa atctttttcg atactctttt
    11941 ttctatcctt taaatctaaa attatactta tgctttcatc taatttatct tttatacctg
    12001 catctaatgg catgtaatat cccccattta tatttcaaaa tcttttttcg ctgaatctat
    12061 actattaatt tctgaccttt tatcactttc tactactaaa gtagcttttc caactggttt
    12121 tataattaaa tcacttagta gtttagcaaa actcttttta cctatagctt tctccatatc
    12181 actaattcct tttaaggtcc ttggcttata aattttttct tcatcataat ctgaatttaa
    12241 taaaacctta gcaacttctt gctcatctac atactttcta ttacttctgc cttctacaag
    12301 cttatatcct ggatatttaa caccatgctt ttcagcttgt tctagtgcat aactttgaac
    12361 atcttttaac caatcttgta tattcttagc aaaacctaaa atatctgcta tctcatattt
    12421 attaagagca aatgtgtcag caaaatcata ttttctagct agtttaaggt tatcctcagc
    12481 tctttttctg caatcatttt tagctctaca aaatccacaa tgactgccac ttacaaactc
    12541 tccttcacca ttaaaagcca tttgagcctt cttcttaaca ttatctgccc atgtaagtaa
    12601 tttagtaact tctatttctt cacttgatat gttatcaagt ctaggttgaa ttattgttgt
    12661 tttaattaaa tcaatatcgt atagcatttc aaactgatta tatgccccta aaccatatag
    12721 tctaagttga ggattctcta tagctgaaac ttctaaacct tttccatact ttaggtctat
    12781 aacttgaagt ataccatcag atattacaac aacgtctcct gttccaaatc cttctggaac
    12841 ccactcacta aaatcgagtc tttcttctaa catcactatt acatcatcac atatagcttt
    12901 actatcattt actaattcaa ctacattctc aacataagat tgtatatagt cctccatttc
    12961 agagttataa tactcacttt tctgtatctt tttaattctt gcattatatg cctttttact
    13021 tattttttca tactctagca ttaatttaac ttctgctaat tcatgtgcaa ctgttccttc
    13081 ttctgcatat atactagttg atggtggata gttttcttct aattttatac taggagtgca
    13141 gtgaagccat ctatgggctc cactcgcact aagtcttgca tgttgtaatg gcatttatgt
    13201 accccctata aactttctaa tttgttcata aaagctgaat aatcttcttc ttttacttca
    13261 cttaacttac tagctccaaa ttcaccaaat aactccttaa gcttatcctt cttacctgcc
    13321 ttacttactt gtgctgcctt agttcttacc tcttcttttg tgtatttaac ttcactagtt
    13381 gtattttcat tattcttttc tatttcttcc tttgcttctg ctatttcttc tttaacgtct
    13441 tcttttactt ctaccttttt aatcttttca gcttttttaa cttcaacttt cttctcttct
    13501 ttcatatctg ttatatttat aggttctatc tgcattgctt ttcctagatt aagacctcct
    13561 aaagcattcg ccactactaa tagtgcattt gtaaattctg gtgcttctac tttaactttt
    13621 acatttacat tcacttcaac cattttaaat ctctcctttt tgtgttatac tttacttgtg
    13681 ttatatttta ttttttattt ttttgtgtgt tggttatttt accaacactt ttttatttaa
    13741 tattccaacc gatattttct taccagtttt aatatcttta aatactatat ctgctataac
    13801 ttttccatct tttttaagag ttactacatt cttattatta gtatcaagac tcagcaattt
    13861 catcccccct ctctactgct tttaaaagtt catccaaatt tttaccttga tttctttcaa
    13921 taaaatcatc aacttcatat cttgaaattt ttctaccatc acctctaact agtgatttta
    13981 tcaaacctgt gcttactaac ctacgcataa aagctgtatc taactttaaa attccccttg
    14041 cttcttctac tgttattaga taatttggat aacctctttt tattaaaaca actatatctt
    14101 taggctccag tacctttacc ttttgttcaa tagtttgact cttaactcta tctgtctctt
    14161 gtttacttat ctctatttct attaagcttt ttaagctatc actaaattgt tttgttatgc
    14221 tttcagaaat atccatgtga aaatgctctc ccctcttttc aattatttat cttccaacca
    14281 attcgtctaa tgtaatatct aaataatcag ctaattttat tagagtatct atagttggat
    14341 tagaactttt atttctcagt aatttatata aaccacttac atctatatcc aattcttttg
    14401 ccaatttata aggttttata tctctttttc ttagtatttg ctttatattg tcgcctatta
    14461 acattgtatt cacctcagtt ctacatggta taatataaac atggaataaa ttccaaaatt
    14521 ttcaaaagga tggtgtatta tgcagttttc aaacaatata tctaaagaat tacaagaaag
    14581 tataagaaag acagtctctg atacttctaa cacagagtat ttttactatg ctgaatttca
    14641 atataaaatt attcttaaat ctatagagga atttgaaaaa gaacttgatg atgaacatga
    14701 aattgcacta aaattgacta actttggaaa agatgtatta atgattgttg aagaagtagg
    14761 ttatcataac ccttgtctaa ttcactacta cggcatagta aatggagttt attctgaaat
    14821 tcttcaacac acatcacaga taaactttat gataacttca gtaaagaaaa ctgacccttc
    14881 aaaacctgct agaagaatag gttttatatt gtaattatca ttcttatttt ctgattcttt
    14941 ctttagtaat ttcactaatt tttctttttt cacttttact catctcatgt aaatacaaaa
    15001 ctatacattg gtacatataa tctataaaat cactatttga ttgagctgtt atctgactaa
    15061 ataactgtgc ttgcaattga ttttcaagca tggttattct tttctctatt tcttctattt
    15121 tcttattttc caattcaatc tctcctttcc aaaatattct gtatttagtt ttcaaagtac
    15181 taacctaata ttgaagtttg ataactatat tctttttcta tatctggtaa tatgttattt
    15241 gttttcaata aatcatataa aaacaatcta cctttttgag tccacttagt tgtcattttt
    15301 acatcaggca ttccattact tctagttatg tctattgttt ctgaatgagt gtatcccttc
    15361 ccttgatgtt gtttatataa aagccattgt ccactttgtt tatattgaat ccctctttca
    15421 tgaagtattt tgttcatttc ttttccactc attccataat cctttgctat ttgagttata
    15481 gtgactagac ctttgttttt aagtatcata tctgtataat ctgcctttgg ttttagttct
    15541 tttattactt ggtctttcat ttttccttct agttgtaatt tctcatttac ttctacttgc
    15601 tctataagat gttgtaatgc ttctttatat gtagttggta gtttaggttg ttcatttttt
    15661 aatacttgct ccatttcttc aaattttttt gtatatattc ctgtaaatgc cgtacctttt
    15721 atgcctgtca ttttatttgc tatgaagtca caacctatct ttgttattgc atagcatggc
    15781 ttctctcttt tataatcatc taaataagta ttttcaataa aataatcaga tgggtacaaa
    15841 tctgtactca tgtttttatt ggcttcttcc atttggttta tataatttct tatatccctt
    15901 agtaaaatct tgtgttcttt ttcaaccatt agagctatat ctctactgtc agttgttagt
    15961 ttattattaa cttttactat ttttaaatct ttcatattta actcctctct tttaaacaac
    16021 atcttgttgt atttgtattt aaaaaaattt tttctattga tactccaaaa catttagata
    16081 attttattgc tattgctaaa cttggtactc ttgttccact ttcaatcatt ccataataac
    16141 tagtagttat acctactttt aatgcaacat ctttttgctt taaattcctt tctgtcctaa
    16201 gttcctttag attgttcaag ctatatcctc cttaccaaca ttttgttgtt tatacttata
    16261 taatacacaa ctatatgttg tttgtaaata gtttttttat ttttttattt cattttacaa
    16321 ctatttgttg tattatttaa taaaggcaac tttattaaga ggagtgatta tatgagcatt
    16381 ttatcagata gattgaaatt tcttaggaaa gaaaaaggtg taatgcaaaa agaaattgcc
    16441 aactatctta acataactac tagtgcatat ggattttatg aacaaggtaa aagaacacct
    16501 accccagaaa tgttatctag tttagctgaa tattttggca ctactgttga ttatttaata
    16561 ggtaggtatg ataataaagc aagcaatatt tctagcaaaa cttcttgtaa taatacatta
    16621 tttcaaaaaa gactaaaaga acttagagct gaaaaaaata tgactcaaga agatgttgca
    16681 aataaattaa acttaaccaa aagcgcttat ggttattatg aacaaggaaa gacagttcct
    16741 gatgcttata tgttatctag ccttgctgaa atatttaatg tgactactga ttatttatta
    16801 ggtaggtcta ttgtaaaaaa tgatatagac actgtagctg cacatagagt taatcctcac
    16861 aaagatttac cagaggaagc tcaagaacaa ctcaatgatt atattgaatt tttaataaat
    16921 aagtataaaa aataaattaa aagtaggtga taaattatat gaaatctata atatcagatg
    16981 tcataaaccg atttaatgct acaccatttt tatttgttgg ttctggttta actagaagat
    17041 actataatct tccaaattgg gaggatttac taaaagtctt tgcagaaaaa attagtaatg
    17101 atgactttat atatacaagt tataaaaata aagcaaaatc agagaaccca aaaatgggta
    17161 taaatcctag aattgcagaa cttattgaaa atgattttaa taaaaaatgg ttttcagatt
    17221 cttctatacg aagcttagac tctaagtatt tagatattgt taaatcagga gtttctccat
    17281 ttaaggcaga aattgctatg tatattaaaa ataattctaa aatagtagat aaatatcaag
    17341 atgaagttga aaaacttagt aatatctcta aaaaaagttt atcaggattt attacaacaa
    17401 attatgattg ttttttggaa actatcgttg ataattatac aacatatata ggtcaagaaa
    17461 atttagtttt ttcatctata caaggtattg cagaaattta taaaattcat ggatgtgttt
    17521 cttctccaaa tagtattgta ataaatgaag ctgattatat agactttgac agcaaaagtg
    17581 cctacttggc agctaagcta atgactatat ttgttgagtt tcctatcata tttattggtt
    17641 actctgttac agatgtaaat ataaaaaaaa tacttaatgc tatagttaat tgtctatcta
    17701 atgaaaatgc taaaaaactc gaggaacgtt tcatattcat tgagtatgaa aaaaacttta
    17761 atgatataga aatatcaagt catacaattg cttttgataa taaaatgatt actatgacta
    17821 aaattaaact ggaggatttt aatttactct atgaggcatt atctgaaaaa aaatcaaaat
    17881 taccagttag aatattaaga atgttcaaac aagaatttta cgatttcaca attactaaca
    17941 aacctactgc taaaattcgg gttggtagtg ttgatgatac aagaataaaa gatgaagatt
    18001 tagttttagc agttggcaag gcaagtgatt ttggtcttaa aggattaaaa ggattaagtt
    18061 ttgatgaatg gtatcgtgat atagtcatga atgacttaga attttcatca gatgaattac
    18121 ttgaatatgc ttatccatct ttaattaagc aatataataa attaccatta aataaacatc
    18181 tttttaatag cacacttgaa tttccagatt ataggacaat tgcactagaa agtgactttg
    18241 aaaatattat tagcaatagt attaaaaaaa atagaaataa tacttacata aaaaatagaa
    18301 gtgtttatgg tatttggaat gatgaacatc aatcatttga aaaagctaca agacttattg
    18361 cttttttaga agaaaaagaa attgacactg aacagttaga aaatctactt aagaaaatat
    18421 ttgaggaaaa tcctaatatt ttagagtctg caaaaacaag cgaaaaaaca aatttaagaa
    18481 ggcttattag aatatatgac tacatgaaat actctaatat gcaaaagagt cttggttata
    18541 gtgagtaaaa ctctacccac cgctccaaaa ctcctttaat cgctttaagc acaaatacaa
    18601 atttagtgct aactatacta acacacattt gtaagtgctt acatatatta tatgtaatta
    18661 tattataatc atatactata atccatattt tgtctacagc taaaattatc caatcttttt
    18721 tggataaaaa atgaatattt agagcagttc acacctgctc tttatatata acaaaaatca
    18781 aaaatacatt ctttttatag ggggatttca atgaacaaac tagacgcact tttagactta
    18841 gcaaataatg aagagataga aatttactac actgacaaaa tagcagatga cataaaagga
    18901 ttgtatataa acagacaagg actaaagatt atatcattac ttaattcatt aaaacaaaac
    18961 aatgctaaac taatagaaat cttagcagaa gaattaggac atcattttac cagtgttggg
    19021 aactatgtat cttcaaaaaa cagttacaaa aataaaatct tgatagacaa aactgaaaac
    19081 aaagcattaa aatgggcatg tgaatttctt ataacagaag aagaaataat acatgttatt
    19141 aattcacacg ctacaagtgt atacgaaata gctgaagaat tacaagttag catcaacttc
    19201 ttactaaaaa gattagaatt tctatcaaaa aagaaaagca tgttggactt aggaaataat
    19261 agatttttag tattaactaa tttgccaaat ttctacatat atgaggatat tttttaaact
    19321 catttattct acttttatag attttttact taataaatat atatttcaat attattataa
    19381 taaactacac ataaaagcta aaaaattgta agaatattaa gaaaataatt aagtaaaaac
    19441 cagataaaca aaattaagat aatattgtac ataacaaaag tatataaaga gcagttaatc
    19501 tgctctttta tataaacacc aaacaaacat acattctaaa agggagggat actattatga
    19561 aaggtggagt aagaaaaaga agtaacaaat ggtattacta ctttgaccta ggcatagtag
    19621 aaggaaaaag aaaaaaagta gaaagagttg gaggcaatac taaaaaagaa gccgaaaaag
    19681 ccttaagaga agcactaaat gaatatgaaa attctggcat agtatttgaa gaaagcaata
    19741 tcagtttatc agactactta gacttttggt acaaagaata tgtcttactt aactgtaaat
    19801 acaacactca agaaagctac cgaataaaca tagaaaaaca tataaagcca aagctaggag
    19861 cttacaaagt aaaagcttta actcctgcaa tactacaaaa cttcataaat aaaaagtaca
    19921 aagaggatta ctctcaaaat acattacaag tattaaaagc catattacat aggtcattaa
    19981 aatcagcagt ccatccttac aaacacatac gagaaaaccc tatgcaatat gtaagcatac
    20041 caaaaactaa atctaaaaca gaaactaata aagttaaaac tattacagta gaagaattta
    20101 atcaaatact aaatatattt cctcaagatt catttcaacg tatagtttta ctaattggat
    20161 ttcatactgg tatgcgaaga ggtgaaatta ttgcactaaa atgggataat atagacctta
    20221 ataataaaac tatcacagta agacatactc tgattaaaaa gccaaatgga atgtttgaat
    20281 tagggcaacc aaaaacagaa agctcttgca gaactatatt tacaggtgac actttaataa
    20341 aggcattaaa agaacataaa ttatatcaaa agaaaatgaa attaaaatat ggagaatttt
    20401 actttgatag tgactgggta tgtaccaaag aaaatggtca acaagtgaat actcacactt
    20461 tagacactat agtaagacaa attcgagtag ctttaaacaa tgacttccat tttcattctt
    20521 taagacatgc acatgctact ctattattag aaaatggtgc taacattaaa gacatacaaa
    20581 accgtttggg tcatagccaa ctatcaacta caatggatac ctattcacat gtaactgata
    20641 aaatgaaaaa tgaaactgta gatatatttg aaaaaattac aaattagagt ttgccaccca
    20701 aaaatataat acggtggcaa atgggtggca aaatctaatt tatctatttt aaaagctaaa
    20761 attatcaaat ttatatagtc agctatacgc atgtaatttc aaggctttag agtatataac
    20821 aaccataact aatataaggt attaataata aatctaatga ataaaactta atatttactt
    20881 aagttgtaat actattctaa attgtataat taaaatttaa taagttctta attatatagt
    20941 cattgattta tttgcaaaaa taaaaaatgc caatctatct ctaaacaaca aaaactgaat
    21001 actaataaaa atttaataaa aaattttatc aatattcagt ataagtttta gttacatatc
    21061 aaattttcca atgcatcatt aggtgctaca ataggataaa catcataatc cttatctata
    21121 tcacattgca aaaatacagg tttattaaaa tctatcatat ctagcgcctt tcctaattct
    21181 tccatactgg ataccttata tccatcaatg ttatatgcat ttacgagttt aacataatcc
    21241 acattttcat taatgtcagt ttgagagtat ctttgattgg aaaataactt ttgccattgc
    21301 cttaccattc ctaatgttct attattgagt agtaaaataa gcataggtac attataattt
    21361 gcaactgtag ctagttcatt acagttcatt ctaaaacttc catcaccagt gactaaaacc
    21421 acatttttat ctacattacc aactttagtt ccaatagctg cacctaaacc aaatcccata
    21481 gttcctaacc ctgctgatgt tataaaactt ttattacctt taaaattcca gtacttagca
    21541 gcccacattt gatgttgacc gacatcagtt actaccacag tgggtttctt taatgtttca
    21601 tatttttcat ttattttttt aagtatattt tgtggatgaa attcatatgt ttgtacaccc
    21661 tcactctttc taaatctctt tatctcttct ttccaattgc tattattctt actttcaacc
    21721 ctttcaatta ataaactaag gactagcttt acatctccaa ccaaagatac attagattct
    21781 atatttttac ttatttcaga tgggtctatg tctatgtgta ttatttttgc atttttagca
    21841 aattcagaac ttttacttat cactctatca ctaaatctcg cacctatcgc tattactaaa
    21901 tctgaattag aaagtgctaa attactttct ctacttccat gcattccaac cattccaaga
    21961 gataattcat tctttctatc tatatttcca agccccataa gtgtatttag tacgggtgta
    22021 tctatcttag ttgcaaattt ttcaagaatt tcctcactgt ctgaagattt aacacctcca
    22081 cctgcatata ttataggctt tttagattct tttatgatat ctattgcttc atttaaaagc
    22141 ttaatattag tttcatcatc tagatcaaaa tcactcttat aatccatata atcatctatt
    22201 tgacataaat catagtcctc accactaaaa tccatttctg ctaaaaataa atcttttgga
    22261 acatctacaa gtacaggtcc ttttctacct gaatttgcaa ctctaaaagc ttcttttatt
    22321 gtaggaacta attctttagt gtttctaact aaataattat gttttgtcat agataatgtt
    22381 gctccagtta tgtcaatttc ttgaaaagaa tcttttccta ataaactagt tggcacctgc
    22441 cctgaaatta caaccatagg tgaagaatcc ataaaagctg ttgctattcc agttatggca
    22501 tttgttgctc ctggaccaga tgttgtaaaa caaactccta ctgtattagt acttcttgcg
    22561 tacccatctg cagcatgaac taaaccttgt tcatgggatg tccttatatg tttaaaatca
    22621 tctgaataat cgtatagagc atcataaaga ggtatcacgg ctccaccagg atatccaaat
    22681 attgtgtcaa ttccctcttt ctttaaacat tctaaaatta cctttgctcc attcatacgc
    22741 actactatac accactcttt ccctcaaact aatccattta atatatattt attttaagaa
    22801 tgacatcata cttcttagat ttctaccaac ttcggcaatt tctgtattat attcttcttc
    22861 tctagttttt aggaagtttt cacgtccttc ttcattttct ttaatccacg cttttgcaaa
    22921 ttttccattt tgtatatcct ctaaaacatt ttgcatacct tgttttgcaa catcagttat
    22981 taccctttta ccagatacat aatctccata ttctgctgtg tcacttatac tatatctcat
    23041 tctttcaaat ccaccttcat atataaggtc gactattagt ttcatttcat gtaaacattc
    23101 aaaatatgct acttcttttt gataacctgc atctacaagc gttttatatc ccaattttat
    23161 aagttcactt attccaccac aaagtactga ttgttctcca aataaatctg tttctgtttc
    23221 ttctttaaaa gtagtttcta aaacacctgc tcttgttgct cctatccctt ttgcatatgc
    23281 taaaactgtc tctgtagctt ttttagtgta gtcttgatat actgcaaata gtgcaggaac
    23341 tccacttccc tttgtaaata catttctaac taagtgacca ggtcctttag gagctaccat
    23401 tacaacatca acaaattctg gtggttgtac ttgattataa tgtatattga atccatgagc
    23461 aaaagctaat gtctggcctt cttttaagtt atcctttaca ctctcttcat aaactttctt
    23521 ttgtttttca tcaggcatta acatcattgt caaatcactt tcttttgtag cttctgctac
    23581 actcttaact tcaaatccat cttcttttgc tttttgtgct gatttacttc cgtcataaag
    23641 acctatcact acatgtacac cgttatctct aagattttgt gcatgtgcat gtccttgact
    23701 tccataacct aaaactgcaa cctttttatt ttttaaaact tctaaatcca catctttttc
    23761 ataatacatt cttgccattt tcatttcctc ccaatacttt ttcttatttt tatgatattt
    23821 atttttatta gaaattcata ctcattatca ttgttttaat attattcaac agatttaaat
    23881 tc
//
LOCUS       DAMJZE010000040        23827 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJZE010000040 DAMJZE010000000
VERSION     DAMJZE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.040"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            274..444
                     /locus_tag="PC541_001484"
     CDS             274..444
                     /locus_tag="PC541_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838952.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            987..1865
                     /locus_tag="PC541_001485"
     CDS             987..1865
                     /locus_tag="PC541_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2838953.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            1901..3796
                     /locus_tag="PC541_001486"
     CDS             1901..3796
                     /locus_tag="PC541_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2838954.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            4064..5971
                     /locus_tag="PC541_001487"
     CDS             4064..5971
                     /locus_tag="PC541_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2838955.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            5961..6410
                     /locus_tag="PC541_001488"
     CDS             5961..6410
                     /locus_tag="PC541_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2838956.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            6510..7397
                     /locus_tag="PC541_001489"
     CDS             6510..7397
                     /locus_tag="PC541_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2838957.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            7802..9250
                     /locus_tag="PC541_001490"
     CDS             7802..9250
                     /locus_tag="PC541_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2838958.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            9276..11666
                     /locus_tag="PC541_001491"
     CDS             9276..11666
                     /locus_tag="PC541_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2838959.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            11668..12339
                     /gene="pgmB"
                     /locus_tag="PC541_001492"
     CDS             11668..12339
                     /gene="pgmB"
                     /locus_tag="PC541_001492"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2838960.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            12378..13748
                     /locus_tag="PC541_001493"
     CDS             12378..13748
                     /locus_tag="PC541_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2838961.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            14095..14937
                     /locus_tag="PC541_001494"
     CDS             14095..14937
                     /locus_tag="PC541_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2838962.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            15082..17010
                     /locus_tag="PC541_001495"
     CDS             15082..17010
                     /locus_tag="PC541_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2838963.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            17071..18498
                     /gene="ascB"
                     /locus_tag="PC541_001496"
     CDS             17071..18498
                     /gene="ascB"
                     /locus_tag="PC541_001496"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2838964.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            18909..19685
                     /locus_tag="PC541_001497"
     CDS             18909..19685
                     /locus_tag="PC541_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2838965.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            19701..20066
                     /locus_tag="PC541_001498"
     CDS             19701..20066
                     /locus_tag="PC541_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2838966.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(20242..20991)
                     /locus_tag="PC541_001499"
     CDS             complement(20242..20991)
                     /locus_tag="PC541_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2838967.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            21469..21894
                     /locus_tag="PC541_001500"
     CDS             21469..21894
                     /locus_tag="PC541_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2838968.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            21926..23113
                     /locus_tag="PC541_001501"
     CDS             21926..23113
                     /locus_tag="PC541_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2838969.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            23305..23709
                     /locus_tag="PC541_001502"
     CDS             23305..23709
                     /locus_tag="PC541_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2838970.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         9516 a         2469 c         4175 g         7667 t
ORIGIN      
        1 aaatgtaata aaatttaatg gagaaatttt ataggtagat aaattttaat atttataaga
       61 ttatgttaag gaaacactta aaaattagat taaaattgtg ggtatttaaa tcaactaaaa
      121 gatattgact atttagtcaa tatctttttt tattaaaaag gaatatacat ataaataggt
      181 tagattaaaa attgggataa atatgattta tatagcatca tttaattaaa aggagctata
      241 ttagtattca ttcatacaat tggataatat acaatgtcag ccttgcttta tggtgtttgt
      301 aataaatcta tttcagtttc tattgtaatt aatatgcaag aatttaagat aggagtatct
      361 atggcttatg gtgtattagt aattggtata tgctatatag cattaggaat aatttttaaa
      421 ttagatagaa aaagatttac ataagataac aaaggctaat ttataaattg gtgcttagaa
      481 aaccaaatca ctttattagc tagagatgaa tttggagggt gtcactttgg tgttttatgt
      541 aaagtttatg gaagtaaaac attagataat cgtgagtaag atttctataa atcgtatata
      601 ctattggaat ccaagaaaag gtattggcca aatagctaat atctttttaa tttttttgac
      661 tctaatatag cattaactat tgcatgagta tctttaaatt tatagttaat gtatcttaat
      721 tgtttattat aaaatctagg aaggctaaag cataaaacac ttaaaaagag aagatgtaca
      781 tgagaaatat aattaactat gatgcagact tctaaaaatt atttagtgaa aaggaactta
      841 gagtgtataa taaaagttcc ttttcactaa ggaaaacgat tgttattttc tgacattcat
      901 taccctgata ttatttaaat ataaacaaca acaaaattaa caagaagcaa attcaaccta
      961 aaatttaaaa tatcgggagg tacaatatgt taataaacat gaaggaaatg ctaaaagtgg
     1021 cacaagaaaa tcaatttgct gtaccagcat ttaatatagg aagtgggcaa atacttaaag
     1081 cagtagtaca gagtgctaac gaaaaaaatg caccagtaat acttgcaata cacccaaatg
     1141 agctaagttt tctaggagat agctttgtag ctagttgcat agaagaagca aataagagta
     1201 aagtacctat ggtaatacac ttagatcatg gggaaaataa agaacaaata ttaagggcaa
     1261 taagatgtgg atttacatca gtaatgattg atggctcaca tttaccatat gaagagaatg
     1321 tagctatatc tagggaaata gtagaaatag caaaaggatt aaatgtgtcc gtagaaggag
     1381 aacttggaac aataggaaca acaggaacat caagtgaagg tggtactgat gaaataatat
     1441 atacagattc aaaattagct aaggattttg ttgaaaaaac aggagtagac acactagcta
     1501 ttgcaattgg tacagcacat ggtatatatc caaaaggttt taaaccagag cttaaactag
     1561 atttattaaa agaaataaga gaagtagtag atataccttt agttcttcat ggtggttcat
     1621 caaatccaga tgaagaaata gcacaagcag ttaaactagg agtttgtaaa ataaatattt
     1681 cgagtgatgt taagtcggca tattataaga agtgcagaga actattagaa caaaacccaa
     1741 gcttatatga gccagatact atatatccac catgtataaa agctgcaaga gaagttatag
     1801 agtttaaaat gaatctattc aatgctatag ataagttaaa gtgcttttat aaaaataaaa
     1861 attaattatg aggtaacaaa ttaaaaggag gggagaattt atgataggta aaatactacg
     1921 aagcgattgt attgtactag ataaacaatt acaaacaaaa aaagatgtga ttaattatat
     1981 ggcaaatcat ttgtaccatg gaaattatat aaataataaa gaaaagttta tagctgatat
     2041 taataaacgt gaaaaagaat catcaactaa tatgggtaat ttaatagcta tacctcatgc
     2101 taagtcaaat gtagtaaatc aacctgtaat gatgtattta agaacagata gagagattac
     2161 agataatgat ggtcaaaaac ttaggtcatt ttttatgata gcagttccag aatcaggttc
     2221 agatgaacat cttaagatta tatctaaact agctacatat ctaatggaag aaaattttct
     2281 taagcatcta ctaagtgcag aaactccata cgaaatatta agtatattta ataataaagc
     2341 tgcagaatca ggagatttta gtaataatca aaataaaggt cttatagttg ggataacagg
     2401 atgtccaaca ggtatagctc atacttttat ggcaaaaaaa gcattagagg aagcagggca
     2461 agaacttgga tatgaagtta aaatagaaac tcatggagct ataggtgtag aaaataagtt
     2521 gactaaagaa gatataaaaa gagctgaata cgtaataata gcttgtgata aagaagttca
     2581 actgagtaga tttataggaa agaaagtgct acaaaagaaa gtagtagatg ctaagaatgt
     2641 ttcattagct aaaaatttaa ttataaacgc tgaaaatgga atggctaaag aaattaaacc
     2701 aggtggaaag actttagaag attcaaaaga aatgcttcaa gggtcagaga aaaagggtgt
     2761 ttataagcac ttaatgagtg gtgtatctga aatgattcca tttgtagtga taggtggaat
     2821 tggtatagcc atagctttta tgtttggtat atttgcatca gacccaactc atgaaacata
     2881 caaccctata gctggattct ttggacaact tggaggcgat tcagcattta agttatttat
     2941 accgatactt gctggtttta tagctaaatc aatagcagga agacaaggat ttgctcctgc
     3001 tatgatagct ggttttatgg cagttattgg aggttcaggt tttataggtg gtatggtagc
     3061 tggttttgca acaggatata tagctttagg agtaatgaag ataacagaaa atttaccaga
     3121 tgtattaaaa ggagtaaatg ctgtattgat atgtccactt ttgattgcat ttacgagtgg
     3181 agctataatg ttctttgtag ttaatacgcc agcatcatgg ttaaatgaag ctttacaatc
     3241 ttggttacaa agtatgtctg gaactaataa agtattatta ggtgcaatat tagcagggat
     3301 gatggcatca gatatgggag gacctattaa taaaacagct tcagcttttg gattggctat
     3361 gttttcaagt aatatatttg aaccatcagc agcattaatg gtaggaggaa tggtacctcc
     3421 attagcaata tcattagcta tgttaatatt caaaaataag tttactaaag aagaaagaaa
     3481 gtcatctcta ccaactgcta taatgggggc atctttcata acagaaggcg caataccgtt
     3541 tgcagctgca gacccattaa gaatgattcc agcaaatata attggttctt gtataggtgg
     3601 ggcaatatgt atgagtttag atataacttt aatggcacca catgggggaa tatttgtaat
     3661 accatttgca tgtagtgcac cagttgcata tatagcttgt atattgattg gtacagtaat
     3721 aacagcattg attatagggg taacaaagcc tactataagg agtgaaaata gtgaagttga
     3781 attacttggt gcttaaatta taaataatag taaaaataat taatggaata acaacaagta
     3841 tatagaataa taataaggat atttagaatc agttaactat ttctaaatat ccttaatttt
     3901 tattttgaaa ttatattatc tattatatca atttagtaag gtgtatcata aattctttta
     3961 tgatacagaa gtatcttagt aattttacat agttagtaaa tatgttaaaa tagtacttaa
     4021 tataaacata gaaatatata aacataggta gggagggcta agtatgcttg tgtcgaggca
     4081 aattgaattt cttaaattga tactaaacga aaataaatat agacctataa aatactttaa
     4141 agataaatta aatgtatctg ataaaacttt acaaaaagat ttgaagatga ttgagaaata
     4201 tttagaaact tttaatataa aaattgatat aaaaagaggc tatggaatat taatagaaga
     4261 tttagcaaag aagaacatag acttaattaa tagtttaaat atacaatcga aggaaaataa
     4321 atctttatca gtagagcaga gaagaatgga gattttaaag tatttattgc taaattcaaa
     4381 taatataact tcaataaatc agttagcaga taaatattat gtaagtaaaa cttctatagt
     4441 aaatgatttt aaatatatag aggcatggat aaaagaatac aatttaaaac ttaataaaac
     4501 tttagaagga actaagatta taggtaaaga agtggatata aggaaatcga ttgccaaaat
     4561 gatggatgat ttattagatg aaaattatga acaacatgat atatctgagt taacaagatt
     4621 agattctgct acatttagtg cattgataaa tttatttgat attgattcta ttatatttgt
     4681 agaaactata attacagaat tagaatacaa tttaggatat actataaatc agccctacta
     4741 tataaattta attacccata tacttatatg tttaaagaga atcgaagaag gtaatcaaat
     4801 agaatctcag gaagaaagag aaatttctgt agataatttg gacgagttag tatataaaaa
     4861 tgtcactaca cttatagata agatagaaaa tagatacaga gtaaaaataa cgaaagatga
     4921 aattatgtac atatatattt ttttggtatc atctggattt agtagcgaaa ataatagtag
     4981 ttacaatgat gaatcagata gattaagtga aagtgaaaaa gtttcatcta taatgataga
     5041 gaatatgtca cgatttttaa atataaatct caaaaatgat gacttactac agaaaagtct
     5101 agcatctcat gtaaggccta tgttaaacag attaagatat gatatccaaa taaaaaaccc
     5161 gttgttagga gagatacaag agcgattttc agatgtgctg ggattatgta tgatgactat
     5221 aaatattatg acagagtatg ataatctgaa gaatattagt atagatgaag tttcgtatct
     5281 tgccacatat tttcaagcgg caatagaaag aaatatgagt agtaaaagag taatagtagt
     5341 ttgtcataca ggatatggaa catctcagct ccttgcagcc agattaaaaa gagaatttcc
     5401 tgaatggact atagttgata tagttcctat gcatcagttg agtaagcgaa atttagatga
     5461 tgtagacttt gtaatatcta ctgtaaactt agatttgaaa gataaattac atatagttgt
     5521 atcagtacta ttaatggaaa gtgatattaa taatataaaa aatgctttac tttctaaacc
     5581 taaaaaaagt atgaacttag atacaaactt attaggaatg tatttagaag ataatatata
     5641 ttttaatttt gatgtagctc agatggaaag tttaataggt gtgaatttag ggagtaaata
     5701 tgaatctatt tcattaggaa aagatttaaa tatatatata cataagcaaa gtaaattaaa
     5761 taaagggata atgaatatca ataatgttac tagaacgatt gatatttatt tagaaattag
     5821 taatcaaagt tttttaaaga atattttaat ggaaatatca aatttatata ggcaaaagaa
     5881 ctatataaac tatataatag attgcaagag caaagaggat attaaaatat tatttaagaa
     5941 taatctattg gagaaaacaa atggaaatta gagaaatatt aacagaagaa agcattattt
     6001 tagatttgaa atcaactaca aaagaaggca taatacagga attagtagat aaattatacg
     6061 atacaggaag tataacttta aaaagtaagt ttataaaggc attaaaacat agagaaaaaa
     6121 taggctctac tagtataggt tatcaaatgg ccatgccaca tggaaaaaca aaatatgtaa
     6181 agaaggcatc catagtaata ggtaaatcta aaaaaggtgt agattggcca agtatagatg
     6241 gcttacctgt aaaggtaata attatgtttg ctatgccaca atctggaggt aatgaagata
     6301 tgatattaat atctaaaata gcttacattt taggtggtaa aaatataggt gaaagacttt
     6361 taaatgcaca tacaaaaaaa gaaatattag atatatttta tgaagtgtag gtattgtata
     6421 aattataaat ttgaggtttt taaaataaaa tgtgtattct tttaaatagt atagattaat
     6481 ttttagacac tagagaggag aggatatata tggcaatata tttagaaatg cctaaattaa
     6541 tacatgaatt tccttttcgt actctatcaa atgaaggaga agtattaaca acaccacact
     6601 ggcataagga aatagaaata ttacttataa cagaaggtgt agtaaatcta tttataaatg
     6661 acaagcctat gcaattaaaa aaaggcgaga tagtgataat aaaaggagga gatattcatt
     6721 atatactgcc atcaccggga agtgaaagat tagtatttca atttgatata tcttttttta
     6781 atgatttaat tttattaaat gaggagaaag aaagcttaat taatttattc agtagtataa
     6841 aaaaatgtag tagagattgg tcagcaaaaa taagaaagtc agttttcgat atattgatag
     6901 atatctacaa tgaagaccta tacaaaatag aaggatatag ttatgcaata aaaggtaaaa
     6961 tgtataattt aattcctata ttatatagac agattcctag agaaattgaa gaaaatacag
     7021 tagaatatga aataaactct aaagagatat tagagagatt gaacaatata ttcaaatatg
     7081 tagaaaaaaa ttataaacaa cctataaaat tagaagatgt gtcttatgaa gtaggattta
     7141 gtgtatatta ttttactaaa tttttcaaaa aaaatacagg aaaaacattt ataacttttt
     7201 taaatgaata tagattagaa aaagcaaagt ggatattact taataacaat atatccattg
     7261 tagatttggt tgaagaagtt ggttttggaa gtacaaaaac tttttacaga gtatttaaag
     7321 aaaaaattgg agtctctcca ttagaattta aaaaaagata taatagcttg gagaaaaaag
     7381 ggagtgtctc aaaatgaact tagttcatga ggcactttct tttatatgaa atcaaatata
     7441 ttttcagtat ttcaacaatg aaaattccat tcatttataa agtctaaatt ttagttctca
     7501 atacaacctc tatttcgaaa taaatccttt tatatatata aatatacagc aatatatgag
     7561 aataagataa caataatgag gatataaaaa tgtatttgct atatatataa ttatatacat
     7621 agatatctaa tattgatatt taatatgaaa gtattgaaaa tagacaatat taattatagt
     7681 gagtaaaaaa tataattgga tttttaaatt gaattattta aaaggtatag tatgaaaaaa
     7741 atcttataac taaatatgag atagaaatta taaaatttta tactgtgaaa gaggtagatg
     7801 catgaaatta gggaaagatt ttttatgggg aggggcaatt tcagccaatc aaagtgaagg
     7861 attttattta gatggaggaa aaggtttatc tcagtttgat aaactcccat taaatgataa
     7921 gagattaaaa aatgtatgtt tagatgagga taatatttta aatgaaaata atcaatacta
     7981 tccaagtcat atagggatta aattttgtga aaattacaaa gaagatatag caatgttagc
     8041 agaaataggt tttaaatgtt ttagattttc tatatcttgg tcaagaatat tcccaacagg
     8101 agaagaaagt gaaccaaatg aaaagggatt acaactttat gataatataa taaaagaatt
     8161 aaaaaaatat aatatagagc cattaataac gataagccac tttgatatac cttattctct
     8221 agttgaaaaa tattctgggt gggctgatag aagagtggtt aatttatatg taaaatatgc
     8281 tgaaattttg atgaaaagat ataaagatga tgtcaaatac tggattcctt ttaacgaaat
     8341 gaatatgata atgcatattc catatatagg tgctgggtta acttttatag ctgatgaaaa
     8401 tcaacttcag aaaaaatatc aagcagcaca ccatcaatta ttagctaatg caaaaactat
     8461 agaaatagga aggtatataa ataaagaatt taagtttggg tgtatgttag ctgctggaaa
     8521 aacatatcca tacacatgta aaccagaaga tgtatttgca actttagagc atgagaggca
     8581 taatttgttt ttttctgatg ttcaagttag gggtgtgtat ccaagcttta tgagttatta
     8641 tttaaaatca aatcaaataa aattaataat aaaagaggaa gatttatcaa cacttaagga
     8701 taatactgta gattttgtat cttttagcta ttattctagt gcttgtagtt cagctagagc
     8761 tgaaggttta gaaaaatcaa aggcaaacgg accagaaacc ataaaaaatc catatcttcc
     8821 aaaatcaaat agtgtatggc aaaacgaccc tgtagggtta agaataacta tgaatcagtt
     8881 atatgaaagg tatcagttac ctttatttat agtagaaaat ggactgggta ctcaagatat
     8941 tgtaaatgaa gatggaaaaa taattgatga ttatcgtata gattacatga aagaacatat
     9001 taaaaatatg attcttggag taaatgaaga tggaatagag cttataggat atcttatgtg
     9061 gggatgtata gatgtaacca gtgtaagtga aggaaagatg acaaaacgat atggcataat
     9121 tcatgttgat tctgatgact atggaagagg ctcatttaaa agaactaaaa aacaaagtgc
     9181 tttttggtat aaaaaagtaa tagaaacaga tggagaaatt ttatttaatg atatggaaac
     9241 tcatgcataa ttcattttaa gaaaggagtg ttattatgag tttaatattt actactaagg
     9301 accaacttgt agagctaggg tttagtaagg atacaataaa atccaatgaa acgttgttta
     9361 cactagctaa tggacattta ggagttagag ggaatattga agagtctgat tttaataatg
     9421 agtatgtaga taatatgggt acatatgtaa atggatttta tgaagaatct ccaattacat
     9481 atggagaaag cgcttatggc tatgcaaaac aaaatcagac tatctgtaag ttacctaatg
     9541 ctcatatagt aaatttctct attgaaggag attgctttga tttatcaaaa ggtattacaa
     9601 gtgaacatac taggatacta gatttaaatg aagggattct taaacgttca tttatatggg
     9661 aaaactctcg tggtagaaaa gttaaagtat ctatagaaag attagtatca caagatatag
     9721 aagaattaat gatgatttca tatacaataa caccattgaa ttttaatgga aaaataaaat
     9781 ttaaaaataa aatggaaaat agtatggaga agtttaattg tgaaaaagta gatgacccaa
     9841 gagtggcatc tataaataag aagcaattta aaattaatgt aaaaaaatat aacaataagt
     9901 attttatgaa tttagttaca gaaagaagta gattagggtt atggtgtggg tttgattcat
     9961 atattacttg tggaaatatt acaaatgaga cctataatgt ttcagatgaa tgtattgagc
    10021 gagaaataga agtctatgcc aaaaaagatg aaaggttaaa tttagaaatt gttgtaggct
    10081 atagtagagt atattctgat ttaaatctaa atgaagaacg tttaaaatca ttagaatgtc
    10141 tggagtcgtt aaaatgttta gaatcagaag cacatttaaa atcattagaa ttggttttaa
    10201 gagaagcatg taatattgga tttgataaag tcaaaaatat tcagagaaag aaaatgaatg
    10261 agttttggac aacctctgat gtagagattg aaggcgataa aactcttcaa ctggggataa
    10321 aaaccaattt atttcatatt tatcaatcag caggaagaga tggaaagact aatatatcag
    10381 caaaaggtct tacaggtgat ggctatgagg ggcattattt ctgggataca gaaatgtata
    10441 tattaccatt ttttatatat actcaaccat ccattgctag agctttatta gattatagat
    10501 actcaatctt agatgcatca agagaaagag ctagagaact tggaataaat aaaggttgct
    10561 tatattcatg gagaactata aatggtaatg aatgcagtgc atattatcca gcaggaacag
    10621 cacagtttca tataaatgca gatatagctt atggtgttag aacgtatttt gaagcaacta
    10681 atgatgaaga atttatgatg caaaatggct tagaaattct tatagaaaca tcaagatttt
    10741 gggttgaatt tggagattat atagaatcaa aaaataataa attctgtata aatggagtaa
    10801 caggaccaga tgaatataca gcaatagtaa acaataatta ctacactaat ttaatggcta
    10861 agtataattt aatatatgca gtacagatgg tagataaatt taagcataat ttattttcaa
    10921 atgaagtatt tagaaaagtt aaattacaag aatatgaagt gaaaaactgg ataaatgccg
    10981 ctcaaaatat gtatttacca tatgatgaag agaagaaaat aagcaaacaa gacgattcat
    11041 ttttagaaaa atcagtatgg gattttaaaa atacaccaaa agaaaaatat ccattacttc
    11101 tccattatca tccattaaca atatatagat atcaagtaaa taaacaagca gatactgtac
    11161 tttcagcgtt gcttttccct gatgagttta gcttagaaca aaagaagaga gattttgaat
    11221 actatgaaaa aataactact catgattcat ctttgtcaag gtcaatattt ggaatgatgg
    11281 caagtgaaat tggaaatcat gaaaaagctt ataactattt tatggacaca gcattaatgg
    11341 atattaataa cctacaaaaa aatacaagtg atggaataca tgctgctaat atgggtggaa
    11401 catggatgag tttagtatat ggatttgcag gaatgaaaat aaaaaatgat aaattaagtt
    11461 ttgaaccaag gatacctaaa cactggaaaa atattaaatt taatatatta tttagagata
    11521 atttagtttt tgtaaatata gaagaatata aatcttatta taagttaatc aaaggtagaa
    11581 atataaccat attacatgat aataaagaaa taaaattatc aaagaacata atagaagtta
    11641 ataattgttt taaagggagt aattaatatg aaagcagtct tatttgatct tgatggagtt
    11701 ataacagata ctgctaagta tcattttgaa gcttggcatg agatagcaaa atcgctagat
    11761 atagaaatag atgaaacttt taatgaaaaa ttaaaaggca taaacaggga agaatcatta
    11821 gataaaatat tgttatctgc taataaagta gatagttata ctaaagagga aaagttaaag
    11881 gtgtgtgaag aaaaaaataa catttatata aagttaataa actcattaac atataaagat
    11941 atattaccag ggataaatga gcttataaaa gaacttaaaa aaaataatgt gaaattggga
    12001 atagcatctg ctagtaagaa tgcacctagg atattagaat ctttgggagt aaaagatgaa
    12061 tttgattgta tagtaaatcc atctttatta aaaaggggaa aaccatatcc tgatattttt
    12121 attgaaggga gcaagatgct aaatttaaaa caatatgatt gtgtaggaat agaagattct
    12181 tattcgggta ttaaagctat aaatgctgcc aatatgacat caattggtgt ggggaaaaaa
    12241 gaagtactag gaaaatgtga ccatattgta gaagatacta gcttattaaa ttacaataca
    12301 ctattacaag cttggagtaa aaagattaat ttggattaaa attatattaa aaattatttt
    12361 aaaagagagg ataaggtatg aagaataaat atgaagatgt agctcaattg ataattgatg
    12421 gacttggagg aaaagataat ataattgact taactcattg tatgacacga ttaaggttca
    12481 tcttaaaaga tgaaaagaaa gtcaatgcag ataaattaaa atctatagat aaggtagttg
    12541 gagtaaactc aacttcaact caatatcagg tcataatagg taatgaggta ggtgttgtat
    12601 ttaatgcaat aataaataag gggataaata ccacaggaga tattaaaagc gatataaaaa
    12661 ataaaaataa aaaaaatagt attttaaata atataataga tacaataact ggttgtatga
    12721 cacctatgat accagccctt acagcatctg ggatgataaa agttatatta tctctttgca
    12781 ccacttttaa ttggctatca tcaaattcaa gtacatatag agtattagat ataataggag
    12841 atgtagcatt ttactttatg cctataatac ttgcaataaa tgcagctaaa aaatttaatg
    12901 ttaatacatc aatagcagtt atagtagtag gtgtgttttt acatccaaat ttctctgcat
    12961 gggtatcaag tggggaccct atatcattta taggagtacc aattcaaggt gttatatatg
    13021 cagcatctgt tatccctgca cttttaacag tatggatgat gtcatatata gaaaatttca
    13081 ttgataaatt aacacctagc atgttaaaaa caattttaaa tccaacttta gtattattaa
    13141 taagtgcgcc aatagcatta atagtaattg gaccaatagg aaatttctta ggagagggat
    13201 tagctagtat aataaattta ctacaaggaa ggttaggatt tataatggtt tgtttattag
    13261 cagctgcaat gccatttatc gttatgacag ggatgcatca tgcattgact cctatattca
    13321 tatcagcatt tgcaactaca ggacaagaat cgctaatatt aatatctcaa gtttgtgcta
    13381 atttagcgca aggtggcgca acattagctg tagcattaag aagcaaagat agtaagatga
    13441 aacagttagc atcagctgca tggatatcag ctattatggg tataactgaa ccagctcttt
    13501 atggagttac attgaaactt aaaaaacctg ctatgttagc atctatagga gcaggaatcg
    13561 cagggttatt tgcaggaatt actcatgtga ctttatatgt tccacaaaat agtataatgg
    13621 cagtacttgg attatcaggg gaaaagggga tttcaaatat agtaaatgga cttatagtta
    13681 tatttatagc attggttgct ccatttatta tgacatatgt ttttggagtt gaaaaaaatg
    13741 ttaaataaag tacagataat gtagtagagg aagtataata ttaactattt acattaatag
    13801 aatttttatt attattgact ataacttgtt ttgtatttat attgatgcaa agaaataagt
    13861 taatattaat aagaataatt attataactg acaaaaagca acataaaaga ttgatgaagt
    13921 atatggaaat aaaaagttga caaaatatgt ctgatgtttt atcatattaa ttggaattag
    13981 gtagtaattg tttaagtaca agctaagctt attcattaat aatagcagta tcctaggaag
    14041 gagtatgccc attattatgg ataagctttt tttatgctta aaaggaggat aatcatgata
    14101 attaataaga tattaaacaa taatgttgta atcacattag atgataatga tgaagaagtt
    14161 atcgttatgg gtaaaggaat tggttatcaa aaatcaaaag gcaatctaat agataaaact
    14221 aaagtaaata aagtatttag aatatcaaat aaagaaatat caaacaaact acaagaatta
    14281 ctcaataata ttcctataga acacatgaaa ttatcaagtg aaattataga atacgcacaa
    14341 ataaagttaa acaagaagct aaatgaaagt atttacatat ccttaagtga ccatacatat
    14401 tctgcaattc aaagaatgaa agaaggaata aatgtaaaaa atgcaatcct ttgggaaatt
    14461 aaaagatttt ataaagaaga gtttgaaatt ggaatgaaag cactagatat tattgaaaac
    14521 aaaacaggta taaaattacc agaagatgag gctggattta tagcctttca tatagtgaat
    14581 gctcagttaa gtgaaggtca tactttggca tctgatatta cgaagttaat acaagaagta
    14641 cttagcatag ttaggtatca ttttaggata gaatttcatg aagaatcagt gttttattat
    14701 agatttataa tgcatttaaa gttttttgcg caaagattgc ttttagatag tgcacatgaa
    14761 ggtgagacag acaaagaatt actaagcatt attaaatcta aatataataa agagtttgaa
    14821 tgtgtagtta aaattaaaaa tttcattaaa aaacaatata actacatatt aacagatgat
    14881 gagcttatat atttaacaat acatttagct aaagtagtta aagactcaaa tatgtaagtg
    14941 aaataatttt tcagcagtat attaagaatg attgaaagga gtgatactaa caaaattata
    15001 cttatggttg gatggtaaca ttgagttggc caaaccttca aatttcaaaa aattaattta
    15061 aacgaaatat ggaggagaaa aatgggaaaa tacaaacaat tagcacaaga gatagtaaac
    15121 aatgttggtg gaaaagaaaa tattagtgga ttagtacact gtattacaag attacgtttt
    15181 actttaaaag atgaaagtat agcaaatgac aatgtattaa aaaacatgga aggtattgtt
    15241 actgtaatga aaagtggagg acaatatcaa gtagttatag gaaatcatgt tgaagctgtc
    15301 tataaagatg tagttgaaat aattggttta gatgattcaa gtacaagttc agaaactaaa
    15361 aaatcaggta atatactaga taaaggtata gatataatat caggaatttt tcagccagtg
    15421 ttaggaataa tggcagcttg tggtatgttg aaaggattta atgcattatt tgtagcaatg
    15481 ggattatata gtgatgtatc tggtggttat atggtaataa atgcagcggg agatgcatta
    15541 tttacattcc ttcctttgtt cttaggatat acttcagcta agaagtttgg attaaaacca
    15601 atgttgggat tagcactagg agctgcagta tgttatcctg ctatacaagg tagcagtata
    15661 tcagcaggag cagatgtgat gtacacttta tttaaaggaa caatgtttgc atcaccagta
    15721 tatattgatt tctttggact accaattgta tcaattgatt atactggaac agtcgtacca
    15781 attatattta tagtgtattt tgcttctagg tgtgaaaaat tatttaataa gtgtgttcca
    15841 aatttagtta agtttttctt tgtgccaatg cttacattat taataacagt gcctgttgca
    15901 cttatagtta taggccctat agcaacattt ggttctacac ttatttcaca aattgtaatt
    15961 tcaataagag attttagtcc actattggca ggtgctatag taggatttac ttggcaaatt
    16021 cttgttattt ttggtatgca ctggggattt attcctgtat atataaacaa tgttatgact
    16081 ttaggatatg ataatgttat gatgccattc tttgcctgta catttgcaag ttcggcagtt
    16141 gtgttggcaa tatttttcaa gactaaagat aaaaaactaa aagagatggc aataccaaac
    16201 tttatatctg gtatatttgg agttacagag ccagcaattt atggaatatt attgccttta
    16261 aagaaaccat ttataataag ttgtattgct agtggtattg gaggcgcttt ctatggattc
    16321 tttaatctta gaaaatttat aactggagga atgggaatat ttgaacttcc agcaatgatt
    16381 gaaccagatg ggggaatggg taacttaata gtagctcttt ctggtatagc tatatcaatg
    16441 gtcgtagctt ttgtattaac tatgatttta tataaagatg agaaagtaga agagtctaaa
    16501 aaagtaagaa ataagggtaa agaagaaata aaagaagtca agtctactaa attagaaaga
    16561 gaaatagtaa caagtcctat aaaaggtgaa gtattaaaat taagtgatat agaggatgca
    16621 gcatttgcat ctggtgtact aggtcaaggg gtggcaataa tacctagtga tggaaaagta
    16681 gttgctccag tagatggggt agttacaacc ttatttccta gtctacatgc tatagggatt
    16741 ctttctgata caggtgttga agtgcttatt catattggat taaatactat tcaattagag
    16801 ggaagaggtt ttgaggcatg tataaaacaa ggtgatagaa ttacaaaagg tcaaactata
    16861 ttaaactttg atgttgatgc aataaaagag ttggggtata gtactgtaac tccaattgtt
    16921 attacaaatt catcacaatt tttagatgta gtagaaacag aaagtaaaaa tattgaatta
    16981 gaagacaatc taattacagt cttattttag taaagattga ggtattagca taatctagtg
    17041 ctttggttat aattttagga ggctttgact atgagtttta agaatgattt tttatgggga
    17101 ggagctactg cggccaatca atgtgaaggt ggatacaatg aagacgaaag aggattggca
    17161 aatgtagatg tatgtccaac aggaaaagat agaacagcag ttattacagg gaaactaaaa
    17221 atgtttgatt ttgatgatga gcattattat ccagctaaaa ctggtattga tatgtatcat
    17281 aattataaag aagatataaa gttatttgct gaaatgggat ttaaagtata tagaatgagc
    17341 attgcatgga gtcgaatatt tccaaaaggt gatgaagaaa caccaaatga aaagggactt
    17401 caattttatg aaaatatatt taaggagtgt aaaaaatata aaatagagcc tttagtcact
    17461 attacacact ttgattgtcc aatgcattta gttaaaaagt atggtgcatg gcgtaatcga
    17521 aaaatgatag gtttctatga gaaattatgc aatgttatct ttagaagata taaaggatta
    17581 gtgaagtact ggctaacttt caatgaaata aatatgattt tacatgctcc atttatgggt
    17641 gctggtattt gctttgaaga aggtgaagat gttgaaaaga taaaatatca agctgcccat
    17701 catgagcttg tagctagtgc aattgcaact aaaatagcac atgaagttga ttcaaataac
    17761 ttaattggat gtatgtttgc agctggaagt gtatatcctt atagttgtaa tcctaatgat
    17821 gtatgggaag ctacaaagtt agatagagaa aattacttct ttgtggatgt acaatctaaa
    17881 ggtaaatatc ctaactatgc gttaaagtat atggagcaaa aaggtattac accagaaatg
    17941 gaacctggtg atattgaatt attgaataaa tatacagtag attttatttc attttcatat
    18001 tacaatagcc gttgtgttag aaccgatgaa aatgcagatg atatggctga aggaaacata
    18061 tttacatctg ctaaaaatcc atatctttca tacagtcaat ggggatggcc aattgaccca
    18121 ttaggtttga gaattacact aaatcatgta tatgatagat atgaaaagcc actatttgtt
    18181 gtagaaaatg gattaggtgc taaagatata gctgatgaaa atggttatgt agaagatgat
    18241 tacagaattg attatataag agaacatata aaagctatgc atgatgcagt ttctatagat
    18301 ggggtcgatt tattaggtta tactacatgg gcacctattg atttagtaag tgctggtaca
    18361 ggtgaaatgg aaaagcgtta tggatttata tatgtagata gagacaatag tggtaatgga
    18421 agtctaagaa gaatgaaaaa gaaaagtttt gaatggtata agaaagtaat tgcttcaaat
    18481 ggtgaagatt tatattagac aagaaatcta tgttaaataa gaaatttgta ttaaataaga
    18541 agagtataaa aattaataat aagtataatt tttaactttt ttaactatag aaaatcaata
    18601 cagaattatt ttacattgta tttatgcaat agtatgattc acaaataaaa ttttagaaaa
    18661 taaaattgtt aagaagtatg ggaagtaagt gcaatttact ttgtaataaa agatgaaaaa
    18721 ggcaagctta ggttttaagc ttgtcttttt tattatgtat aaactatgat actttaaaat
    18781 tctatgaaaa atttatttaa tagagaaatt aaaaatttaa tggtaatcaa attctttacc
    18841 aatgaatata aaaaatgtct acgtgttata atagtttaaa ctaataaacg aaaggtagat
    18901 gaatgaatat gaaataccaa catataatag aagttgacaa agaactttgc attggatgtg
    18961 ggctttgtaa aaatgattgt ccagtaaaca atataattat agaaaataaa aagtcagtaa
    19021 ttaaaaaaca agattgtcta atgtgtggac attgtgcagc aatatgtccc acaaaagcta
    19081 ttaccttgac aggatttgat gaacctccta tagaacttac aaataagcca aagctagatt
    19141 cagatgaatt actaatggca attaaatcta gaagaagtat acgtaaattt aaagacaaag
    19201 aagtttcttc agaaattata aagcaaatta ttgaggcagg taggtatacc cctagtgcta
    19261 aaaattcaca agatgtatca tatattgttt tagacaataa aaagtcaatt tatgaaaacg
    19321 aagcagtgaa attttttaga aaaataaaac ctattgcaaa tatagctatt aaatattcaa
    19381 aagaagttga gattgacgat aactttttct ttaagcatgc tcctattgca ataatgataa
    19441 ttacaaaaga taagattagt ggttcacttg cagcatcaaa tatggcatta atggcagagt
    19501 cttatggcct aggtgtttta tttagtggat atttttcaga tgtagcaaat aattcgccaa
    19561 aattaaaaaa gcttttaggt ctaaaacgca gtaatcatgt tcttacaaca ttagtaattg
    19621 gatacccaga tgttaaatat agacgtactg cacagaaaga agtagcaact gttagatact
    19681 tataagagag ggtggatttt atggaaaaag aatgtcaaca aagaatgcaa gaaataacgt
    19741 ctaaatttaa agaatgtcgc aaagctatct ctgctatggg tgatgagact agacaattga
    19801 ttttaattgc tctcttagag agtgatttta atggtatacg agtgggagaa atcacaaaaa
    19861 aaacacatct ttctcgtcct gctgtatctc atcatcttaa gatattaaaa gaagcagaga
    19921 ttgttaattt gcgtagagaa ggaacaaaaa attattatta tcttgattca aaaaaatcac
    19981 aatggaggtc actaacagag ctaataaatc ttatatttgt tggaatacaa catattagtg
    20041 aaaatgatat aagaaaaggt gattaaatta tacttgattt aatacaaaaa gattgattta
    20101 ggaactaaaa agaactaaaa agttcacttt gagacactat cttttgaact atacccatct
    20161 taggagacag ttaaaatatg taaaactgta taaaacaatg ggtatagttc attttataat
    20221 ggtttaaata ttaagaatat attattctaa atagtatctt tctctgtttt gagttttatt
    20281 tttatactca atagcttcag tggggcaacc acaaatacat gccatacaat gagtacagtt
    20341 atttttccaa actggttttt tgttttttaa attaatatta tttaaaggac atagctctac
    20401 acattttcca cagccaatgc atttatcagt ataatgaaat cctttggcac taataattgt
    20461 cttataaaaa actgtattta ttataccact tttaattgta cctcctaagc cactaggagt
    20521 aataatttca aattcatttt catttcgtat atcagaaata atcctatata tttgtttatc
    20581 tgcttcttta atcatttgtt ttgatgtttc tttgtctgtt gtagaaaata aagctatata
    20641 attctctggc attttaattt ctgccatacc ttttaattgc cattccttgt atttacatag
    20701 tttttgaata tagtttatgg attttgctgt atctccacca caagtcatta caaagtaaac
    20761 tctgttgctt cctagaaatt ttgtttcttt tataaaatcc gtaaccacta ttggcaatct
    20821 ccatgcataa gtaggacata caaatatgaa tggtttatct gttgatatta attcatctgt
    20881 tttttcctct tttataagtt gatttagtga tatcagttca tcatttaatt cttgtgcaat
    20941 tttacgagcc acatatcggc tattaccagt tccgctaaaa tataaaatca taataatatc
    21001 tccctaacta ttttttattt taactcttaa ttttttattt attatagtaa tttattgata
    21061 tattctattc aaatgtatgt aaaatttagt agttaagaaa attataacac attagtaatt
    21121 tttatactta ttggtaaaga atttgattac cattaaattt agcaacaaga catcaactgg
    21181 acttgtatat gagaagggat aataatttgc ttatgaaaga ttataaatat aaaggttatt
    21241 ttgaaggtgt agaaatattt gataatgtaa atgaccttaa cttaaatact ttaacaacag
    21301 ttagtagatt tgtgtttgtt tttttgttta cgataactta aggaaatatg ttacctgaac
    21361 aataaattta tataataaaa gtatataaat atatatatga atgttaagat tagttgatag
    21421 aatttaaata ataaatccaa tattataaac ataggaggta tgaaaaatat gattaatgag
    21481 aatttaaaaa gtctaagaaa aatcaatcag tatacacaag aagaattagc tgaaaaactt
    21541 aatgtttcta gacaatcaat agcaaaatgg gaaagtggag aatctattcc tgatataggt
    21601 agttgcatta aacttgcaaa attgtacaat gttaaactag atgacttagt taatcattcg
    21661 gaagaaaaaa caggtattat agtacctcct aaaggaaagt ttttctttgg agcagttgta
    21721 gttggtgaac gtggtcagat tgtcattcca aaagaagcaa gagaagtatt taatataaat
    21781 gcaggagata aattattggt acttggagat gaggaaagag gattaggaat tgttcatcaa
    21841 cgtgatttga taaattttat aggtcatgtg ggagtagtac ataaagaaga atgatttaaa
    21901 aaaattataa aaggaggtat gaaatatgag taaaatagta ttttttagca ttcctgcata
    21961 tggtcatact aatcctacta ttgaagtagt tcgtgaatta gtggatagag gaaatgaggt
    22021 attgtattat tcttttaatg aatttaaaga taaaattgaa ggtgctggag caaaatttat
    22081 ttgttgtgat aaataccttc cagaattact ccctggagat gagaaaaaaa ttggtaagga
    22141 ttttcctgga ttaattgaga tgatagtaga tactactatt tctttagatg aaaaagtttg
    22201 tagagaactt aaagatttta atccagattg cattgtatca gattcattaa gtttttgggg
    22261 aaagttattt gcacaaaaac ttaatattcc atatgtatgc tctacaacaa cttttgcatt
    22321 taataaacat acagcaaagt tgatgaaaca aaactttata gaaattatac gtatgatttt
    22381 tggtgtaaga cgcattaata aaaaaataaa gcttttacaa agaaaagggt atgaagtaaa
    22441 aaattttatt tctatagttt ctaatgataa tgatacggat accattgtat atacatcaaa
    22501 agaatttcaa ccaatggtag aaaccttctc tagtaagtat tcatttgtag gtccatcagt
    22561 atcaaagttg attattgaac ctaaagagag aaaaagaaag ctaatatata tttcgcttgg
    22621 tacaattaat aataaaaatg ttagctttta taaaaactgt ataaatgcat ttaaagattc
    22681 tgatgttgat gtgattatgt ctgttggtag aagtactgat attaaaagtt taggaaatat
    22741 accaaataat tttgaggtta aaaatagtgt tgaacagatt gctatacttc aaaaaactga
    22801 tgtgtttgtt actcattctg ggatgaatag tgttaatgaa agtttatatt atggagtacc
    22861 tatggtatta tttcctcaac attcggagca gagaatggta gctaagagag ttgttcattt
    22921 gggagcagga attatgttaa aagaggataa atcagaaagt attaaaaaag cagtatttca
    22981 agttataaat gacaatgaat ataaagaaaa tgcaactaaa ctatcaaaaa gcttttataa
    23041 tgcaggaggc tcaaaaaaag cagcagatgt aatattacag attatatgta atagtagaca
    23101 agttgacata taatagattt gttaaaaata tatatacata catgtaattt aatcaaagta
    23161 ctgacagtgg ggtcttttaa cctaccagtt ggggcatata tattgatgct aaatatgagg
    23221 gatgttatat tttaataaaa aagataacat ccttttttta gtgcattata tcgagtttta
    23281 aataataaat gaaggaggat taatatgaaa aaatataaag ttatttttat agtattaatt
    23341 ataggtatta taagtgtaac agggtttgtt gtatataaaa actgcattaa accatggaaa
    23401 gatgctgaaa catatattga taagtatatg gtaaaacaag atatttcaaa agataatata
    23461 aaaagtatta caaaagaaaa agccaagaag gcaagttacg aaggaatact atacaaagtg
    23521 tattacaaag atgaccctcg atatgagtat caatattttt atagtgataa ttattatgct
    23581 ctatatgtaa ataaagtaat gttacaaatt tatgatatgg aaaataataa taaactttta
    23641 agaagtaatg aggaattaaa aagtgtaaag tatccaccaa tatatcttaa atataagcca
    23701 gataaatgat ttatgacaaa atagaataat ttttattaaa atttatttaa aaatatcact
    23761 agagcgttta aataaaacta aatttaagta tttatatatt agtatatctt actagtgttt
    23821 aaacaaa
//
LOCUS       DAMJZE010000041        23757 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJZE010000041 DAMJZE010000000
VERSION     DAMJZE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.041"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            238..1212
                     /locus_tag="PC541_001503"
     CDS             238..1212
                     /locus_tag="PC541_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838971.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1328..2389)
                     /locus_tag="PC541_001504"
     CDS             complement(1328..2389)
                     /locus_tag="PC541_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2838972.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(3001..3852)
                     /locus_tag="PC541_001505"
     CDS             complement(3001..3852)
                     /locus_tag="PC541_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2838973.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3869..4789)
                     /locus_tag="PC541_001506"
     CDS             complement(3869..4789)
                     /locus_tag="PC541_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2838974.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4899..5813)
                     /gene="murQ"
                     /locus_tag="PC541_001507"
     CDS             complement(4899..5813)
                     /gene="murQ"
                     /locus_tag="PC541_001507"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2838975.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5912..7009)
                     /locus_tag="PC541_001508"
     CDS             complement(5912..7009)
                     /locus_tag="PC541_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2838976.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7242..8588)
                     /locus_tag="PC541_001509"
     CDS             complement(7242..8588)
                     /locus_tag="PC541_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2838977.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8677..8985)
                     /locus_tag="PC541_001510"
     CDS             complement(8677..8985)
                     /locus_tag="PC541_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2838978.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9248..9646)
                     /locus_tag="PC541_001511"
     CDS             complement(9248..9646)
                     /locus_tag="PC541_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838979.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9648..10808)
                     /locus_tag="PC541_001512"
     CDS             complement(9648..10808)
                     /locus_tag="PC541_001512"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2838980.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11239..12258)
                     /locus_tag="PC541_001513"
     CDS             complement(11239..12258)
                     /locus_tag="PC541_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2838981.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12252..12932)
                     /locus_tag="PC541_001514"
     CDS             complement(12252..12932)
                     /locus_tag="PC541_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2838982.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(13084..14991)
                     /locus_tag="PC541_001515"
     CDS             complement(13084..14991)
                     /locus_tag="PC541_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2838983.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(14992..15744)
                     /locus_tag="PC541_001516"
     CDS             complement(14992..15744)
                     /locus_tag="PC541_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2838984.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(16060..17214)
                     /locus_tag="PC541_001517"
     CDS             complement(16060..17214)
                     /locus_tag="PC541_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2838985.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(17217..17684)
                     /locus_tag="PC541_001518"
     CDS             complement(17217..17684)
                     /locus_tag="PC541_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2838986.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17698..18186)
                     /locus_tag="PC541_001519"
     CDS             complement(17698..18186)
                     /locus_tag="PC541_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838987.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18200..19531)
                     /locus_tag="PC541_001520"
     CDS             complement(18200..19531)
                     /locus_tag="PC541_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2838988.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19545..21110)
                     /locus_tag="PC541_001521"
     CDS             complement(19545..21110)
                     /locus_tag="PC541_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2838989.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(21280..22065)
                     /locus_tag="PC541_001522"
     CDS             complement(21280..22065)
                     /locus_tag="PC541_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2838990.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(22232..23431)
                     /locus_tag="PC541_001523"
     CDS             complement(22232..23431)
                     /locus_tag="PC541_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2838991.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         7913 a         4086 c         2460 g         9298 t
ORIGIN      
        1 ttttcatttg ctatttaaaa tcaattttaa gttttcttct tataataaac ttcctctttg
       61 tggtctcatg ttagtctcta aatttaaaat tttacaaaaa attttcataa aataaaatta
      121 tattgattgt atctaaacaa atagggcaat aaaataatta agtatagtta cttacctttc
      181 aagcaatatt agttttatgt tagaatttta ttatattgat ttacaggagg tttgcatttg
      241 aataatatta attcacttaa agtaataaaa ttatctcttg gatgttctct tgctatattt
      301 attgcttggc tattaaaatt agaatattca atggttgctg gagttatagt actacttaca
      361 gtaaaggaca ctaaaagaga aactcttaaa ggctccattg gaaagattta tggttttctt
      421 ttatgtacta ttttttcata tttatgtttt aatattttag gatataattt gacttcattt
      481 tcaatattta tatttataat tataccactt tgttttttgt taaatattca agatgttatt
      541 gctatgtgtg tagtcatagc atcccattac taccttcaag gagaaacttc tatcaaatgg
      601 attttaaatg aagctggaat atttgcagta ggaactggaa taggtgtctt aataaatatg
      661 tatattccaa caaatataca taaaatccat gaagttcaaa aaaagttaca ggaagaagtg
      721 agtattgtac ttatagacat tgcagatatt attgtaaatc ctaaaaaaga aaatggatat
      781 agtagagatt tatatacatt aaattcactt attgacagca gtatttctga aacttacgat
      841 aatattaata atactttatt aagtgatact agattcttct tagagcatat ggatattata
      901 aaaagtcaac gggatatctt agaaaatttg tatagttatg tatctcaact aaattctaca
      961 ccaccacaag ctcatattct atctgctttt atacataaga taggttatac tgaatttgaa
     1021 gctgaaacag gcaatcttct tcttgaagaa ttaaagagac taatgcttag tatgaaaaac
     1081 cagcctctac ctgttgacag aacagagttt gaaaatagag ctattttatt cctatgcttg
     1141 actgagttaa aacagttttt agttaatcgt aaacatgctc agatgcttag agataataat
     1201 ttttataaat aaaaagggga gaaatctagc agttgacctg actacaaaat atcagatata
     1261 atattaaatg cgcccctagt aagagggcgc aatgtttata acttattcta ctgtcatgat
     1321 taaatcatta ttcatatcga acatacatta tttgaccaag tccaatatta gctttttcat
     1381 cagtgatttc tattttaata gtcacatcat tttttttaca gtacttcgct aattctgcac
     1441 cagaacgatt tttaccacta aaatctaaag aaaatggctt atcatattta aaaggatatt
     1501 caacaggttt accctctggt gctaatgcaa acatcccata tggtgtgttc ataaatccta
     1561 ttacagcaat cgtaagattt gatgggtcta aatttgattc actacctgta aatgtaattg
     1621 tatttactga tgaattaact acactacatc gaacaccttc atacattaca tgtttttcat
     1681 ttatatcttc tccttttttc ccaaaatttc caacagcctt tgccccttct tgatgtggta
     1741 acatagtgtg cttcccaggt ggtgctggtt tctgtgtctg ttctggtttt accgtctgta
     1801 ctattgcacc aacttttgga tatgctgtct tatcaaatgg taatgggtgg ctaagactct
     1861 tgtatatgat atcctttcct gctgatttat tagcatcaat atctacatga tacaaacgcc
     1921 catcaccaaa acggataaga ttccactggt ggtcatttga tgtgctgtgt acataataag
     1981 attcaattcc catagcatca cacaaagctt tcatgctcct agccattcct gagcatgcac
     2041 cttttttagt aaccaatgta taggcagtat ttgctccctc tactgcattc cagtcaacat
     2101 atgttgtgtt atttttaatg tgattgtagg caactaaaat cttagcatct gtggtcatat
     2161 tctttgtgat tttcttgttc ataaaatctg ctgttgcttg tgctactgag tttagttggg
     2221 cttgtgacag ctttggacca tatacgctat tggttccagt gacatagctt tttggtgttt
     2281 ttgctgttac ctctttggct gtcataccat aaactgtcat actcatacct gagattaaaa
     2341 ctattgttgt aatcaataac ttggtagcta acttcctaat tttcttcata cttaatattc
     2401 tcctttataa aacattgtta cttcctattc tctaccatta ataaaattaa tttaggattt
     2461 aaaaaggtgg attgtcaaca tgtatatttc tccattcata cattcgtgaa atatttaaat
     2521 ttcatggaaa atctaataga tattttctag ataatttgta tacaatacaa ctcgcaaatc
     2581 agggtgtttg ctgttagttt gagaaaatga tgatgattaa tacattcgga atctttgacc
     2641 tataataaat accattgtct tcaatcatta ataatggtaa gagtaaagag aattatcaat
     2701 aatacagtta aaatcaactt tgtatacatt tcatacattc tctcccaaca acattatact
     2761 tcttataatt tgtatattca atacgttttc tgggaaaata taggatatat ctataaaaat
     2821 ataaaaaagg agttgaactt aaaatttgat taaaaaaaat caattttaag acaactcatt
     2881 tgtattataa aaatgctata tattattatt aagtaagtca atattttaga tatttatcta
     2941 tttcttattg acttacgctt atagtctaac ttatttgttt agttgatata taacctagtt
     3001 ttaagcatta ttaatattta aataatcaag tatccttttt gtttcttgta gtttctgttc
     3061 tgcctcattt aagttttctt gaattataga aatatatatt atatctatca acgtaagcat
     3121 tgctattctt gaagaaatag ctccttctct aagccttttt tctatatttg gtactactag
     3181 ttttaaatca ctaatcttac taagatgatt gtcactatat ttagttatac aaatgacctt
     3241 acatcctttt aattttgcat tttctataga ctttattact tcttttgatt ctccagagta
     3301 agatattgct atagctatat cttctttatc tacatttgaa gataccataa gttgtgtatg
     3361 actatctaaa gaggtaaatg cctgtttatt tatccttaaa agtttcatct gtaaatctag
     3421 tgcaactaaa gccgaaccac ctactccaaa tatatataca tttttagcct ttattatctg
     3481 ttcaactgct tcttttatta tatctttttc attcaacaac cttgtttggt tgatagtctc
     3541 tatgtttttg tttgtaattt tgtttataac actatctata ttacaatcat agttttcatc
     3601 tacatatgtg tattcatttt ctttgcccat caaatcttta gctatctcaa tttttaaatc
     3661 cccaaaaccc gaatatccca tttttctaga aaacctaact atacttgctg tacttgtttt
     3721 tgagtttgac gccaattctg atacagaaag cttacttata gcctctgtat tattaagtat
     3781 gtaatcagct atttttttat ctacttccgt taaatcttca tatatctgtt ttaattttaa
     3841 aatgcaactc atactctatc acctctacct atttactata atatttcttc ttagcaagta
     3901 aacatgctcc taaatctgga gtcaacactg gtctcactat accaaatctc gttttattca
     3961 aatatttttt aagtggctct aatattaaat ctcctgactt aaacactcca cctgaataag
     4021 atacctttat ctttgtacct tgttcaaaat gatgctctaa tcctattata agcctactta
     4081 attcataagc tgcatcatca aatattttac tagcagtatt atcaccttca ataactgctt
     4141 ttaaacatat cgtagcaaac tttgctattt ctattctatc tccttttatt tcatcattta
     4201 catattttat tatgtcataa tcattctcta tattaagatg tctttttatt aaatcatata
     4261 attttgtttt tgaacacctt ccatcagact gcattgtgaa catttttaag gtagccattc
     4321 ctatataata cgctgaacct tcatctccta atacatagcc ccatcctcca catctgtgaa
     4381 gattcccatc tttatctaaa gcctgtgcta ttgaaccagt acctgcaatt atatttatac
     4441 ctttttcccc atttaaagaa cctgctaatg ctatttctac atcattacct agagtataat
     4501 ttatatgact atatgcattt tttgttgcta cttctaatgc atacaatact tcttttattt
     4561 ttccatatcc agcaagtcca acaaatgcat acgttatttc ttctacattt atcttagcat
     4621 ctttacaaac ttcttccaac ccagttatta ataatttcgt tagattatca aatcctattt
     4681 gattataatg acaagttcct ttggttatgg ttcctactat atttaattct tcatctacta
     4741 atgtaaatgt tgttttcgtt cctccaccat ctacacctaa aaaatacata ctatcctcct
     4801 acaatatctt taataattct ttcaatactt aatatctaaa aataaccttc caatgctgtt
     4861 tttttaatat tgaaaggtta tcagtactat tatttatttt acaatttttc tattgatttt
     4921 aaagcttcac ttatataacc ttcttgtcta tctaggatac tctttgcact atccttatct
     4981 aaaccacttt ttatcataaa tatagccaat ttaacgctat tatctgcttc ttctaagaat
     5041 ttttctgcaa catcatattt tacatttgta gctatcatta ttattctttt tgctctttca
     5101 accaactttt catttgatgt cttaacatct accatcaagt ttgagtatac ttttcccatc
     5161 ttaaccatag aagctgttgt taacatatta cataccattt tttgagctgt accacttttc
     5221 attctagttg aacccattat tacttctgcc cctactataa tgtttattgg taaatccgct
     5281 tttttactca tttcactatt cggattcata gttattgata gtactgcaca ccccaattcc
     5341 ttagcatact ccattgcacc tataacataa ggagtccttc ctgatgcagc tataccacat
     5401 ataacatcct tagatgtaac tcctctatct attatatctt gctttccaag ttctttatca
     5461 tcttcagcac cttctattgc tttaaatatt gcttgatttc caccagctat tatagcttga
     5521 acaagctcaa aatccactcc gtaagttgga ggacattctg tggcatctag tattcctatc
     5581 ctcccactgg tacctgctcc tatatagaat aatctacctc cctgtttcat tctatctact
     5641 attatattaa cagcttttgc tatactctcc ttttgttctt caacagcata ggctacagtt
     5701 ttatcttcgt tatttattaa ttctattatg cctaatgtat cttctttatc tatttgcaat
     5761 gtattcttat ttctaccttc tgtaactaaa ttttctaaag tttttattaa catatcattt
     5821 cctcattttc ttaatattaa atttttaaac acattttaat tccaactttc ataattatat
     5881 ttttataata aattaatatt ttcataataa attattttgt tgagggttta aaattaaact
     5941 tttgccatgg tttaatataa tctaatataa ataattcttc ttttcttata tatcctacta
     6001 cattcgattt accactgttt ttcatatcag tcaaggctat ttgtaactcc cctgcataat
     6061 gaccatattc actactttca attactatat ctcctctttt tattatttta ggagtattaa
     6121 atagttcaaa tttatatcct ttatatttaa cacgagattg agtagacctt atcagactat
     6181 cagatatgtc ccctctatta aaatgtattt cttcaaagag tatcttcttc tcaacttctc
     6241 gtatattttc ctctattgct acatcaaatg taaccatatc ttttctcata gaacctaata
     6301 cctttagctc ctcatatgtt ggataacaat ttgatattat tatatcattt atattattca
     6361 tagctattat atgctttact tgaatatcta taggtagatt tctgtgcatc tcaattgttg
     6421 gaagcccatc agttacaggc catggtccaa atgtagtttc attttgactt gttacaaaaa
     6481 ctgctgttct taatccatac ttattaaatc tttctgtaca ttttataaag tgttctaaat
     6541 ttaatccact atatctatgt ggataaaagt tatgacatcc tatcaaattt tctttatttg
     6601 gttggtagtc cattattgta tctatataat tagtattatt actcatattg atttctattt
     6661 taagattttg aggattaaaa gtcattaatg cctcttcaga acctgtaaat cccatatcaa
     6721 gccttattcc gtctgccttc gtctttttaa aaaaattcaa atctttgtag cttatttcta
     6781 attcaccaaa tactctggga tttacatcaa gtattacctc aaaaccttta aacttagcgt
     6841 attcatttat acttttaaat tttttaatta tattttcttt agtatcttta actgataata
     6901 aacatgaaaa tatacgtgta aatccatttt cagcagcttt atctatataa tcatatattt
     6961 ccttagatgt tgttttttct ggataaattg atattcctaa tcttcccatc tgtgtcatct
     7021 ccttatatcc tatatgtgtt taaatttatt tcgaattaca cgaaactttt ttaaaaattt
     7081 gatgaaaaaa agtttcatat atatttataa aaaaagaaaa gtttaccttt tcttttttta
     7141 taaacaatca acaaaatatc ttcgttactt atttcataac tgttatactt tgaaatttat
     7201 actaatctaa tatagttttt aatttccaat atagtttcta attaataagc ttcttgctct
     7261 tcaacttttt cttcatgaag caacctctta tcgtacatct tgacaaatgg atagtaaatt
     7321 attattgata tagctataca taataagtta agaactgaag ctctccaatc tccaccagtg
     7381 gctaaatatg cccctactgg tcctggtaat gtccaaggag cagtaactac tactctggat
     7441 actaaattag cactcatagc aaaccatgaa actgttgcca ttactattgg tgctacaaca
     7501 aatggtatta ttaatattgg gtttaatact attggcgctc caaatattat tggttcattt
     7561 atattgaata ttgctggtat tacagttgct tttcctaaag actttccata agctgatttt
     7621 gctctaaatg caagaagtaa agctaagcct attgtagctc cagaacctcc aatccatata
     7681 aaccattgaa aaaagggttc agcagcaata cctggaactg cttgacccgc agcatatgaa
     7741 gctgtattag catctaataa ttgtatccat aaaggtcttg ccaatgaacc tattattgat
     7801 acaccatgta ttccaaatga ccaaaaaaac gttattaaaa atattagtat aattactgat
     7861 ggtaatgtgt cagtggctga aactaatgga gttactaatt tagctatgaa gccatgccaa
     7921 tcaaacccta ggaaataagt aacaatagcc attaaaatta ttattaatgc ggctggtgtc
     7981 agagcttcaa aagaccttgt gactgatgct ggaactgctt ctggcatggt tatcttgaat
     8041 ttagatttgt cagtaaatct ataaatctca acacctatta tagatgttac aatagcaaca
     8101 aacattgaac ctccaccaag attagccatt ggcatttgcc atccttgagg aattgaatta
     8161 taccaagcta aaagttctgg actcttttca actatcttta ataaatcagg ggtaagaccc
     8221 tctattgcag taggaactat tgtcaaaagg aatgtagctg tagctaataa acctcctgtc
     8281 aatccatcta acttatatga ctttgccaaa ctataaccta tgccaaatgt agaatatact
     8341 gatataataa aaaatgtcat tctatatggt agtaatattg taactgcatt ttcctttaga
     8401 tactgagtta ccccccatga atctggtaat ggaggaaatg ctaatatcaa gaaaaatgac
     8461 ccaactatta taagtggtaa tgaagctacg attccatctc ttacagctct taagtgacgt
     8521 tgttctgcca atctggccat agaagttgat aatttagtgt ctaaaaattc tgtaaattta
     8581 tctagcatct tttctctcct taattaatat ttatttttat tatcattaaa atttttataa
     8641 aattaacttt cttctaatct aacaacactg caactactat ttactcaaca gctcttgaac
     8701 ttgttttaat aactttggtc ctcctaatgg actataagct tgtgcttgta ttaatgcaca
     8761 tggtacacta acctcatcag caaaagcttt taaatagtca aatctatgtc taacttgtgg
     8821 agctaccata gcaatatccc atccattttt aacttcatcc tcaaactctt gagtaccaac
     8881 agccaaaaca tctatatcta ttccattttt tgctccttct ttttgtaatg cattaactgc
     8941 tatagcactt gacatccctg atgaacaaac aaataatact ctcatattga ttccccctgt
     9001 ttttttattt cttttcaatc attatagcat gtatttttga ttacaggaat acgtttgtat
     9061 atattttttt catattttac attttaaatt gtaattttat ttcatacatt tatatcttgc
     9121 tttaattgct ttatttatct aatcattctt ttgtctaaaa ttacacacta cttaaaatac
     9181 tcatatcttt tcttaaaaaa gaaaaaagac tatcttatta ttaacataaa atagtctcaa
     9241 tactttttta aatatgtatt cctttatata tccagaagta acctattatt gcaaatgata
     9301 aagcaaataa aatatacagt ctaaaaaatc ccaaagaaaa acctctctta gtttcaacaa
     9361 agtatcttgt atatgcaact gcaaaagatg taattaatga atttcctata acaactccaa
     9421 atctattatc tattccaaat atagataata acaaaggaaa aactacagta cttactagag
     9481 atgaaactat taatattaat ccacttttaa aacttatagt tggaatatta taatctgttc
     9541 tttgattttt ctccatttgt ttcttagtat ttttattact ttttgtactt tttgcatttt
     9601 tataattatt atttttcttt cttttctttt ttttattatt agacatgtca tttacctcca
     9661 aaaggtttag gtgttatatt ccctaaaata actttatttt tagcccctgt tgcagatatt
     9721 acattagaaa aagaattatt taatgtttca ttgcccaaga ttacaaatgc caaagcctcc
     9781 tttgcatcag aagaataccc atattcatct tgagttaata cttcaacctc taataataac
     9841 tcttttatat atcctattaa agttagatta tgagctcctc cacctccaat taataaagta
     9901 tctacattaa cctttggaag tatgaagttt ttataattat aatatatagc ttttgccgta
     9961 aacatagtta ctgttgctat caaatcatat ttatctacat gtttatattt gttaagcatg
    10021 ttatccaaat aaacttgtcc aaatacctct cttccagtgc ttttaggtgg cgcttgacta
    10081 agatacttat gactcatcaa atcttttaac atttcttcat taattttacc ttttgatgca
    10141 aagtatccat ttttatcata ctttctatta aataatcttt gacatactcc atctattatc
    10201 atatttcctg gtcctgtatc aaatgcaaat acatcatcta tgttacatga tttaggtatt
    10261 atagtaatat ttcctattcc accaatattt tgaagtgcta aatttttatt gttatcacta
    10321 tataataaaa attcactata tggtactaat ggagcacctt ctccacctgc tgctatatcc
    10381 attactctaa aattagatat tacctttgta tttgtttcat atgctataac tgctggttca
    10441 cctatttgaa gcgttgaaga tacaaaatta ttataacttc ttggtatatg aaagattgtt
    10501 tgaccatgag atgcgataaa atccaaattt gctatatgaa agtttgcttt atgacacact
    10561 gattttacag ctttactaaa taaatatcca agcttaaaat ttaagctaca gattaaatca
    10621 acactcgatt cctcttctat acagcacttt cttatttcgt cttttatatc tttaggaatt
    10681 tctagagtta taaattctat ttgcttaact ttagtatttc taccattccc actaatttca
    10741 gctagtactg catcaactcc atctaaggaa gttcctgaca ttaaacctac actatacata
    10801 gttatcatcc ttttaagttt atagttattc ctccccaaat caaggtatta agtttaataa
    10861 ttttagaatt aatcttattt catctttaat tacgataatt tactcttatg aaattaattg
    10921 agttaatctt atacacgttt tttaccttag ctaaatctta cattaattta ttttcagtgt
    10981 caatgtaata ccatgtaatt ttttttcaca aacaaaacat attctctctc caaaaaccat
    11041 attaaaatag ttactacaaa taaaaaacat accaggtacc tatctgatat gtttttcata
    11101 tataataatt attattttat aatatagata gagcatcatt taaggcacta attaaatctt
    11161 cactattttc aagaactaaa tttattctta ttgtatatgt cgatttgtat atgatgatag
    11221 attttatagt ctaataacct aactatctag gcttaaataa tccgatatat cataaaaata
    11281 gatattaaaa attgtatatg aaccattttc cgaacatacc tcaatctcat gccctaaact
    11341 atctatcatt ttctttacaa aatacatccc cattccagta gacttataca attgatttct
    11401 tccatttttc cccgtaaatc ccttgtcaaa aaccctagat atatcttcct catttatacc
    11461 tataccaaaa tcttttatag ataataccac tccattttct aacttttttg aattaaattc
    11521 aatcttacca acttcctttg aatactttat agcattatta ataatttggt ctaaaacata
    11581 tgacatgcat ttagaatctg tgtaaacatc attttctaat tcattcaaac taacttctac
    11641 attatttttt ataagcaaaa atgaattatt ttttatagac atttttatca atttttctaa
    11701 attaaattta tttataaata catcttcaaa catagtgttg cttctactac tatacaaaat
    11761 agagtttact aaaaaattta tctttgcaac ctgattctta atatccaaac tatcttctat
    11821 gctacttaac ctatctgtaa ttatacttaa agaagatatt ggaattttta tttcatgaac
    11881 ccatttagct atgtactcat ccaattcttt taatgattgt ttataagtct ctatgtcact
    11941 attatttttt atttttaagt tttctataat atcttttatt agattttctt caaaagattg
    12001 tccatcaact aaataactgt ctatttcacc actattttct atacaattat ataaatttgt
    12061 gtaactattt ctaaaattta tataatctat aacaaaaaat accattatta taaacagtac
    12121 taaaaaatct aaatatatta actctgaata tttattttta aaaatatcta tagcaaataa
    12181 gtagatattt attatactaa ctacaactaa aggtagagca atatacttag acctaccttt
    12241 caaatatttt actagcattg tattatatat cctaccccct tcttagtttt tataaaatca
    12301 tcaaaaccta gttctttaac tttacttctc aatctattca tatttacagt aagtgcattg
    12361 tctgtcacaa actcatcatt atcccataga gtcatcataa gactttccct tgatacaacc
    12421 ctattcttat tttttaataa taatgttaaa atttttattt cgtttttagt caactctata
    12481 cttttatcct tatacttatg ttttataatc cctttcccca catcaaacat caaatcttca
    12541 caatacaaaa tactattaga atcactataa ttatagactc ttcttattat accatttatt
    12601 ttggtaacca atatttctat agaaaatggt ttggtaatgt aatcatctgc tccattattt
    12661 atacccatta ttaaatccat atcagaatct cttgatgata aaaatattaa tggtacattt
    12721 gaaatagctc ttattttttc gcaccagtaa aatccattat ataatggtaa atttacatct
    12781 aaaagtacta catctggttt tttatctata aattcatcta atactacctc aaaatcatca
    12841 attaattcta ctttaaatcc ccatttttca agtgctaatg caatctcttt tgataaatca
    12901 atatcgtcct ctactaataa aattctatac atttgtatat tctccattat cctaatattt
    12961 cactatgcta tgaattatta atatcataac atattttata tgaatttatt acccaaatta
    13021 tttagttaat caacaaggag actcttatta caagaatctc ctttctcttt attaatatat
    13081 caactaattt ttatacacta cactcttaaa cttattaaca gatactgctc catatattat
    13141 gaatattatc aaacatatca ctaaacttag tatatatata ccaaataaat cggtcatcaa
    13201 taaatctcct gctaatctca atgcaaaata actagatata gttgcactta acattggcaa
    13261 agtataaaac atagctactt ctttaagtat agctctattt atatcttttt ctgtagtacc
    13321 tatttttaac aaaatcttat atttctcttt atcttcatat gcatcactat atattttcat
    13381 atatattata cttccagcta caattgcaaa gactaagaat aaaaacacaa gcactacata
    13441 tgcaaacttc atccattgac tctcaccttg agctttatat ccattttgaa tatacccatt
    13501 ttttaaactt aaattctttt caatattgtt gaacattact tgactatttt cttcttgaaa
    13561 tacctttgca ccataaaaat acacttgttg acccttatct cttagtttaa aatatgtatc
    13621 atcatttacc actataatac tttgattaag tcctgtccct agtactggaa cttttacttc
    13681 tccccttgaa atattatata actgaccatc aagttttatc ttggcttctt ttgtagctat
    13741 tgatgctatc gtttgagggc tttttaactc tattacatta tttccactaa ctaatctttc
    13801 accatactct tttaattctt tatcattgcc attcacttta agagtattac aaaaatcact
    13861 atatttcatt atagtatctg ttgtatcata agcttctttt atatctgttt tctttaaaat
    13921 tgttagttct ggatttactg agtactttat cttattagta tcttttatta tattctctat
    13981 ttgtttttta ttcacttcat ctttatccat tgaaactatg ctaatagtgt acacatttct
    14041 ttgttcttgg gcattcttat gtatacgctt catcgcaact gaagcaccaa gagttgatat
    14101 tgttgtagtt ataattatag ctattatagc ataagtttta tagtttttat ttaatcggta
    14161 gtttatattg tttattgcaa ttatattatt tccattaaat aatatttttt tatttttgat
    14221 tataatatca aatataacag gcataactga tttgaataat ccataagtac ctactattat
    14281 tatacctata acaggtagta cactagttat atctccagtt cttatattgg tagaaagata
    14341 gtagccatat cctattacta taattgatac tatagctaat atatatatac ctattcctac
    14401 ctttggtatt ttttcttgtt ttttagatat attgattaga tttattacac tacttctaca
    14461 tattgttata aaacctttta cagccattat tataaatata gaaataaaga ttaaagtagc
    14521 atatagtata gcttctatac taagtccatt agataccttt atattaaatc cagataactt
    14581 tattatcact acttgaaaaa atctagatgt tattatccct ataattattc ctgttataca
    14641 agaacttatt cctataagca tattttctac aaaatatatt tttcctatag tatataaatc
    14701 taatcccata aaagacaata ttccaatttc tttagaccta ttcttaaaaa atatatttgt
    14761 tgcataccat ataaagaaaa ataaaaatac tgatagtatc attattacag ctactaacac
    14821 tgatagtgta aattttttat tttcatcttg taacacattt atagcatcac catatcttaa
    14881 taattcaaaa ttagttagca caactactga aaaaaccata gatataaaaa atgctagata
    14941 agttcttatg ttactcttaa aattattata agcaattcca agtatactca cttacatctc
    15001 acctcccata gaggctagca tatccataat ttttccataa aattcttttc tactatttcc
    15061 tttgtttaat ttacacttta tatttccatc atttatcata tatacttcat ttgaataact
    15121 tgctgttaga gggtcatgtg taatcattat aatagtagcg ttattttctc tgtttatttt
    15181 ttctaatgat tctaaaagtt catatgctga tttagagtct aaagcaccag ttggctcatc
    15241 agcaaatatg actgttggat ttgtaattaa tgacctagct gctgcgcctc tttgtttttg
    15301 tcctcctgat aactgatatg gatatttatc taaatgattt tctaatccaa attttttagc
    15361 tatagaaaat accttagctt ctatctcctt acttttcttt tttcctaatg ctagaggaag
    15421 tgctatattg tccattagtt tcatattgtc taatagatta tagtcttgaa atataaaccc
    15481 tattttttct cttcttaatt ttgatagttc attatttttt acttttgata tattaattcc
    15541 atctagtatt atttctcctg aagtttgttt gtcaagtgta gacattaaat ttagcaatgt
    15601 tgtctttcct gcaccagatg gacccattat tgatataaaa tcaccttcat tgactgtaag
    15661 agatatattt tttaacacat ttgttttaaa tccttttatt ccataatctt ttgatatatt
    15721 ttttattttt aatatttctt tcataataat agcctccagt tgctaatttt ttaatatata
    15781 tttattttaa aggttttata taaataaatc cttaagtaaa tattacattt taactgttag
    15841 cattacactt ttgtaacata tggaagacta ttataattat agtagaaata tcttaaaata
    15901 aaagcatgat ttacttcttt gtataaacaa agaaaagaag ctcctaagaa gcctctaatc
    15961 tatacaatta ttcttctcta taaaaaacca aaatacacat tgattgtggc aaatacagat
    16021 actataaaca agtgatatta ctactatata ttaaaacaat tattcctgaa gataattcaa
    16081 taagactatc aagcaataag ctaagcatcc aaaaagactt aataccatta atgtataatt
    16141 atacccaata ttaatgctca aaaaaccagc aacagttgga ataatcagag atgaacctcc
    16201 cataaatata cttaaagcta ttaattggct actacttcta agttttttag gagaaacttc
    16261 aaatattaat ctttttgaag taattaaaat taaaggagta gaaaacactt gaaaaacaca
    16321 cagtataata atgatatttg aatcatttgt taaagcaaat aatagaaact gaactgttat
    16381 cataatagca cttaatttta ataagtttaa actgctatat ttttttaata aataagaacc
    16441 agccagatat gttggtatct ctaacaatga ctgtaaagac cactttaaag atatctgaga
    16501 atttgtagca tttaaagcta tcattttatc tattacagta cagttatttg ctaccaccat
    16561 tgagtataat aagaataaaa tacatactag taagatatat tttttatctt ttaataattc
    16621 aataatatct ttattagtta catttatttt cttattaatc ttttcaatat caggtaataa
    16681 tagtatattt ataataagta atactacaat taaaccaatc gctacagata atccaaaata
    16741 tgagaatcta agaatcaagt aagatgctac catacttcca atagcccatc caatcgaccc
    16801 aaaagtctta ataaatgaca aataattatt aatattatct ccacaaccta ttatccatgt
    16861 atcatataaa ctacagcaag tatttaataa tcctcctgat aaagcaacaa gtaataagta
    16921 tattataaaa gttgcagatt cagtagaaaa cataataact gatgcaattc cataaactga
    16981 taaactaatt aaaacaattt ttttcattgt ttgatactta tcagctaata ctccaaataa
    17041 taattgaaac aatatattgg ttattgcata gctcgataat ataattcctc tttcaaacgc
    17101 tgagtaacct aattttgata agaaaggtgt gaattttgtt gaagcaaagg caaaaataat
    17161 atatgagaaa aaatagaata tgataaattt aaatttaatc gctttattaa tcataatcaa
    17221 tcaaaattga taactttaat accactattt ccttcctgat ttaagtttga aagatgatat
    17281 tttaccgtat taggattctc tacaataact aaaggaatat ataaagtatt ttcaagtaac
    17341 ttattatcaa tttctactaa cttttccccc atattaactt cttgaccttg tttaacttct
    17401 aatttaaagt tttctttttt tgttgttgca ctatccaatc caatatgaat aagaatttga
    17461 acatcatttt gcaatttaat tcctatagca tggttagttt gagcaataaa acttattact
    17521 cctttagtcg gtgatttaag aatattgtcc tttgctatca tagcaaatcc atctcccatt
    17581 accttagttg aaaatacttt atcaggaatt tctccaagat taattatttt tccatcaatg
    17641 tatgaattaa ttgtaattgc ttcttttttt cttttaaaga acattataga cactcctcta
    17701 cataattaac tttattctaa ttatcattac aaataacgat attaagcata ctgcaattga
    17761 ggaaaaaaga gccttatagc tctttcccca ttgtagttct ttaatactgc ttatgatttg
    17821 tttaattcca acaaatttat aaatataggt aaacatgata acaatagtta ttgtgtaaat
    17881 ttggaatatc tgccctaata agactaaata gaaagcaatc atgttttctt ttatctcttt
    17941 attctttttt atattgtaaa taacatatgc actgtatact gcactaaagt tagagattaa
    18001 atttaaactt gcattaacat cattgtttaa aagttgtgaa aatgaaatat ttgtaaataa
    18061 tgatgtcaat tctaaataca ctatatataa aacaggtaat attagaaaac aaaccataac
    18121 cactgaaaga aatatatttt catcttttaa tactgctgtg atatttttta ttttatttgt
    18181 tatcatgttt ttgttctcct tatttaaaag ttggccagaa atccttatta gccacaatca
    18241 tttcatctaa gatttctttt gctacttttg cacttggaac agttttactc atagtaaacg
    18301 actgccataa tttttgataa cttttttcta tccatgcttc tacagctaat ttttcagaag
    18361 caacttgttg ttccattaaa cctttttgga aagttggaat tttacctata gacataggtt
    18421 caaccatatc ttttccaaca aggcaaggaa tttcaaccat tgcatcatct gcaaaatttt
    18481 caatagctcc attattagga acaatcagta aaaatctttc ctgagtatta aatgctaatg
    18541 cacaagctaa ttcaacaata aattgagaat gttcacttgc atcaaaccat gtatctcttg
    18601 ctgtgccatt ttttataatt ctttcacatt cttcgaatgt atccttttct cttctatcca
    18661 taatttcatt tgctcttgta taatttggat tagcatgttc aacagtttca tcagccatta
    18721 aataatattt taaataagta ttaggtaatg tagtcggttc tatttccaat aaatcctttg
    18781 ctgctctcat agtttctaac caactagatt ctttgtgttg accttgaggt gtaaattcct
    18841 catatccttt tttagcacaa tattcctgta atttaggcat taaatcattt ccttctttgt
    18901 ccttaatact tgtccaccac ccgaaatgat tcaatccata atatctaaca tcaaagtctt
    18961 tacgagaatt taaacctaaa actctagcaa atatttcctc taaacatact ggcatatcac
    19021 aaatattaat aattcttgaa tttggtctta atacacgaca tgcttctgca actatagctg
    19081 ctggatttga ataattaagc atccaagcat ttggagaata tttttccata taatcaatca
    19141 tttcgattat tggacctatt gttcttaatc cataggcaat tccacctgcc ccacaagttt
    19201 cttgaccaac aaccccatat tttagtggaa ttttttcatc ttgttcacgt aaagcatata
    19261 aaccaacacg aatttgtgcc attacaaaat caatatcagt aaatgcttct tctggatttg
    19321 ttgtataact aaatttaatt tctggatttt tctctctaac aattattgca catgcttcag
    19381 caacttttct ttgtctctca tcattattat catataattt aattgattca ataggaaatt
    19441 tagcaacact ttcaattaac attgttacaa ttccagcagt ataagtacta cccccaccag
    19501 caattagtat cctctgtctt tttctttcca tctttccttc tcctttataa taaattttca
    19561 aattcttctc ttacacttgg tactgaaagc ccaataataa tctgaatagc ttttccatta
    19621 tgcactaaac catgtgcacc tactgcctta aaatcttctt ctttagaaat aattgagtca
    19681 tctttcaagg ttaatctcaa cctcgtagca cagtttgtaa catctacaac attttctgct
    19741 ccaccaacta attttatgta ttcagatgct tgttgagagg ctttactcaa tttatttcct
    19801 ttttcatctt tagtttctaa gtattcattt ttagtatata gttttgtttc ttcactatct
    19861 tcctctctac caggagtttt catatcaaac ttcttaatta agaaagtaaa tacaaagaac
    19921 cagataaatg aaaatataat ccctacaaca acttgaacaa catatacctt ccagtgatac
    19981 attccaagag gaagccaatt taatacaacc cagttaataa ttcctccacc aaaatcacca
    20041 acaactccta gggcaaacga aatcgtttgc aagcatgcat ctaaaaatgc atgtactagg
    20101 aataaaagtg gtgctacaaa taaaaatgta aattctaacg gctcagtaat tccagttaat
    20161 actgcagtaa gagtagcagg aatcattaaa cctaatactt ttttcttttt ttctggcttt
    20221 gcagttacat agaaagcacc aaatacacct aatgaaccaa acaccttcga taatccggat
    20281 aatgaaaatc cgattggata catttccttt agagtcttag ctgaagtttg aaaatcctga
    20341 atatgagtag cccaatatac tgatgtacca cctggaacaa caacactatc ataacaaatt
    20401 ggtgcatata caaaatgatg taatccagtt ggtattaaaa tcttttgtaa gaatgcataa
    20461 caccaaactc ctatatttcc actatttata aaaaacgttt gcaaatgtct aataacattt
    20521 tgaatatgag gccaaataaa acaagttaat aatgcaactg gtatcataac aaagaaacaa
    20581 attgcaacta caagtgatga gcctctaaat aatccaataa aatctggcag ttctttatca
    20641 taaaatctat tatgaatata aatgacaata cctgatatta acaaagcacc aaccatacca
    20701 gtatctaatg ttttaatacc agcaatgctt acaagtccac ttccagcact tatttcagct
    20761 gcgaaatcaa ctccaaagct ttttccccaa aaacttaaca tagaacctac aaaataatta
    20821 aaagtcaaat acgttgctaa agcctccata caagctcttg cttgttgctt ttttgcaagt
    20881 gctattggta aagaaatagc aaataataat ggcaattgat taaataccat ccaagctcca
    20941 ctagatatta caccccaaaa ttggtaccaa atattttctg gtgtagcaag tgaacccata
    21001 attgactgat tttgaaatac aattgagaat ccaaccatta tcccagcaaa agcaaataat
    21061 agtgttggtg taaacattgc ccctccaaaa cgctgtattt tcttcatcat ataaattact
    21121 cccctcctca tatttttagt ttatctttgg ccttagatat ctattatttt aacaggttaa
    21181 taaatatata caatctttag ggtacattcg gagcttattc acagtcaaat aagtttttgc
    21241 acatttttat gttcaaattc tcataaaaaa aaagaatgac tacaatttgc aatcaatccc
    21301 ttctgtaatc attctatgcc ttttataaat actatattta ataaataata actcaatcaa
    21361 tataaaatat aacattgtca atttataact agggtgcatt tgtaaaaatg agatattagt
    21421 cgctgaaata taaattgatt catcacttaa atcagtcaat gtattatttt taaactctgt
    21481 aattgaaatt attttagttc ctattaattt taatcttcta gcaatattaa gtgtattctc
    21541 attctcacca cttagggata taataatcat aacatcattt ttactgacaa tattgtaaaa
    21601 agaatcatca aatgttcttc cctcaaaatc atatattatt tcttgacatg ataagaacat
    21661 tctctttatc tgttttgcaa ccacattttg aatatcacct gaaccataag cgtatattgt
    21721 tcttgattca taaagaagtt tacacaatgc atcataatct ttattttttt gatcttcaac
    21781 atacttaatt attgaattac atgcaatatc aaatgactga ttttctatga catgaacctt
    21841 ttctgattcc cagcttaata atactttaaa ctcactgaaa ccttttaaat ctaatttttt
    21901 aacaaatctt gtaatagttg cacgagacac attacatttc atagcaagtt catttattga
    21961 catcttacta caatgttttt tatttgcagt tatatatttc catattaata aatctgtatc
    22021 attcaattta ttataattta aaaaaatcat ttcttctaat ctcataaact attaccccct
    22081 ttaattaaaa atcgtcataa atattatacc aatattcaac attaaattac agcgtattct
    22141 tatatttact atttagccca taaaattgac tataaaaaat actgctataa ttgatgttta
    22201 aaaaaatgta gttttaattt tattctttta tctaataatt aatattttca agttctagtg
    22261 cttttcttat acaattatca tctttaaaat cagcagtcct tttacaatct tttactttaa
    22321 attctggaat agttttaaat tcctcatcac atattccatt cttatttaaa tacatacaac
    22381 ttgccattct aactattaga ccactatttt ttaaattaaa caatacaggg tctatacacc
    22441 ctccatctcc gccagtagtt tcaccaacta aagtcgcaaa tttattttct ttacaaaaca
    22501 ttgcaaatga ctctgatgat gaatatattt ttctatcaac aagtaagtat atattaccct
    22561 taaattttga atcatctgta gattttatag tataggaagt atcttcaaaa tcattaaaat
    22621 cttttataat ttctttaggt gcattttcct ttacacttgc tggtaaattt tctacaggat
    22681 ttaattttat atttcttgca tttgtatatt tttttactat ttcattatta tttctataaa
    22741 ttctataacc atttattttg acatcatttt ttatgagttt agatacgatt ccctgccaat
    22801 acctatcact tccacctaaa ttacctctta tatcaataat taaagcttta tgtttttcta
    22861 aagtgttaat ataatctcca attattttta aatcattatt tatagagcca ttctttgatg
    22921 acattgatgg taaatacata tatccaatct gacccttaat aacatccttt aaaattaatt
    22981 ctttttttac aaaaatatca tctggcattt ttattttaag attcatagag tcatatctat
    23041 ccacaacttt tttatcatta agaaaatcat accaatcatt attagagtat gatttcttaa
    23101 aaagttcata tcttttctta ttatctatta cttctgtgtg tttattattt aaatcagata
    23161 atatagatga cagttcttct ataaattctt catcattttc agtgtttttt attcgttttg
    23221 tatattcatc cttatttgct aaccaatcaa tattattaag cctcttattt acacctagat
    23281 atggatatcc tgtctttaca acattataca cataattaaa gtcatccacc ttttcctctt
    23341 cagacaactg attcttttga gaataagtag aattacctaa taatattata aatgctaaaa
    23401 taattactaa aaataatact ttctttttca tgtaattctc atccactttc agattttata
    23461 ttttaataca ttgtatcatt attttattat cacctaaaca gttaaaagaa attcttgaag
    23521 atatattcca ctattattct ctttaaaaat ttttatttgt attttcaaaa ttatttcatg
    23581 aaagttataa aataacaatt atattcaaat atctatttta ctgctatatc ctgaaagtaa
    23641 attcttatca gtgatatact aaaaaagata tgctaaaaaa tttatccatt ataatgactt
    23701 aaatttagat actttactga tttttattta ttagttgaga aagatattga taattaa
//
LOCUS       DAMJZE010000042        23331 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJZE010000042 DAMJZE010000000
VERSION     DAMJZE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.042"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            301..1533
                     /locus_tag="PC541_001524"
     CDS             301..1533
                     /locus_tag="PC541_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2838992.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            1547..2353
                     /locus_tag="PC541_001525"
     CDS             1547..2353
                     /locus_tag="PC541_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2838993.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2465..3334)
                     /locus_tag="PC541_001526"
     CDS             complement(2465..3334)
                     /locus_tag="PC541_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2838994.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3706..5133
                     /locus_tag="PC541_001527"
     CDS             3706..5133
                     /locus_tag="PC541_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2838995.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5210..5479)
                     /locus_tag="PC541_001528"
     CDS             complement(5210..5479)
                     /locus_tag="PC541_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2838996.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5482..6924)
                     /gene="nhaC"
                     /locus_tag="PC541_001529"
     CDS             complement(5482..6924)
                     /gene="nhaC"
                     /locus_tag="PC541_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2838997.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6949..8115)
                     /locus_tag="PC541_001530"
     CDS             complement(6949..8115)
                     /locus_tag="PC541_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2838998.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8340..10460)
                     /locus_tag="PC541_001531"
     CDS             complement(8340..10460)
                     /locus_tag="PC541_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2838999.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10693..11094
                     /locus_tag="PC541_001532"
     CDS             10693..11094
                     /locus_tag="PC541_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839000.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11137..11430
                     /locus_tag="PC541_001533"
     CDS             11137..11430
                     /locus_tag="PC541_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2839001.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11445..11885
                     /locus_tag="PC541_001534"
     CDS             11445..11885
                     /locus_tag="PC541_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2839002.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11977..12450)
                     /locus_tag="PC541_001535"
     CDS             complement(11977..12450)
                     /locus_tag="PC541_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2839003.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12450..13238)
                     /locus_tag="PC541_001536"
     CDS             complement(12450..13238)
                     /locus_tag="PC541_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2839004.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13566..14810
                     /locus_tag="PC541_001537"
     CDS             13566..14810
                     /locus_tag="PC541_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2839005.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15133..16230
                     /locus_tag="PC541_001538"
     CDS             15133..16230
                     /locus_tag="PC541_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2839006.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16221..16919
                     /locus_tag="PC541_001539"
     CDS             16221..16919
                     /locus_tag="PC541_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2839007.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16922..17986
                     /locus_tag="PC541_001540"
     CDS             16922..17986
                     /locus_tag="PC541_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2839008.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18169..18873
                     /locus_tag="PC541_001541"
     CDS             18169..18873
                     /locus_tag="PC541_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839009.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18866..20188
                     /locus_tag="PC541_001542"
     CDS             18866..20188
                     /locus_tag="PC541_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2839010.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20375..21748
                     /locus_tag="PC541_001543"
     CDS             20375..21748
                     /locus_tag="PC541_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839011.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22120..23058)
                     /gene="pdaA"
                     /locus_tag="PC541_001544"
     CDS             complement(22120..23058)
                     /gene="pdaA"
                     /locus_tag="PC541_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2839012.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8600 a         2900 c         3685 g         8146 t
ORIGIN      
        1 attttacata aacttggttg ttttagaaac tacttctgta acgagtacac gattttaatt
       61 aaagaatata taacaaattt gaaataagtc taatatatga cttaatagat taaattaaaa
      121 taataaattt ggactattta tctatttata acttaaatta tatcaaattg ataacaaact
      181 agatattata cttttttgta gaatagaatt acaattatat aaagaaaaat tctattaata
      241 gtgaaaaaaa agtagaaaaa agtagaaatt attcttttat aaagaaaggg agggataata
      301 ttgcaagaga gagaagaatt agttttaaat ccagttccaa aagaggaaag agttggatgg
      361 ttagctccat tgtttaatat gcttggttct aacatagcta tatctgaact aatggttggg
      421 ggaactttaa ttttaggaat gactctttct aatatgatta ttacatcaat tatagggaac
      481 ttaatattag ttgcaattat tatgatacaa ggttatatag gttacaaaga agggttaaat
      541 acgtatgtat tagctaaggg ggcatttgga gagataggag gaaagtatct tatatctcta
      601 ttacttggta taactagttt tggatggttt ggtgtacaag caggggttgc aggactatca
      661 gtacaaaaga tattcccaag tgtaaaccta acattagtta cagttatact tggattattt
      721 atggttgttt ttgcactgta tggttttaaa gctatggcta aatttaatta cttagttata
      781 ccaccactta tgatattaat ggtgtgggga gtttttaaag ctttttctac ttatggagtt
      841 gaagctattt ataattatac tcctcaaaca actatgagta tggttgaagg tcttaatatt
      901 gtagtaggtc ttgttatagt tggtgcaatt atatcacctg accagttaag atatacgaga
      961 cgagtaaaag acatttggat tatcagtttt ttaggtctag ggattatttc attattccaa
     1021 caagttgcag ctggtgtaat gtctatggga gcacctactt gggatataac agaagtattg
     1081 gctaatttag gttttggatg ggtagcgttt gttattttaa tacttgcatc ttggtctaca
     1141 aacgtatcaa atgcttattc tggtggatta gcattaaaaa ctatttttcc aaatgtaaaa
     1201 agaaatgttt tgactttagt tgcaggtcta ataggtacaa taatagcagc tactggtata
     1261 atatttaaat ttcagtcatt tttatctttc ttgggaatcg cagttcctgc aatagcagga
     1321 attatgtggt gtgagtatta ttttatacaa ggaagaactt ataagcatag agaaggaata
     1381 aattggattg cagtaataag ttggttaata ggttttgcag cgtcgtatta tagttcaaaa
     1441 ataaatttct taatacctcc gataaatgga attgtagtga gtatggttat ttattatata
     1501 ttaatgaatt gttttggaat caaagataaa taaagggggc aattacatgg gtcgtaaggt
     1561 gaaaataggt ataattcaac aacacagtgt acttggaaat gttaaaaaaa atatagaaaa
     1621 agcagtagag atgatagatg acttaggaaa acaaggggca gatataattt gtttaccaga
     1681 actatttgct acaggatata atttagagtc tttaggtgga gttaaaacat tagaattaat
     1741 aagagaacat aataagtaca tagaggaatc aatgtcagaa gctgctaaaa gaaataatgt
     1801 atacctgata tctccatatg gaaccttaga aaaagggtct acacatgttt ataattcagc
     1861 agttatattt gatagaaaag gtaagattat gggagaatac tgtaaaaatc atctgtggtc
     1921 attagaagca gtatacttta aaggagggga aaaagtagag gtatatgatg ctgattttgg
     1981 tagatttggt gtaatgatat gttatgatgc aggttttcca gaagtatcca gagaattgac
     2041 attaaaaggt tctgaaataa tatttatacc atctgcatgg agaatacaag atgaagatat
     2101 gtgggattta aatgtatctc aaagagcttt agagaacact gtatatacag taggtgtaaa
     2161 tttagtaagt aatgactcta atttaatact atttggaaag agtaaaatat gcaatcctag
     2221 aggtactgta ataactcaat tagatacata cagggaaaaa tatgtattat cagagattga
     2281 tttagatgaa ataaaaaagt atagggaaga tatcccctat ttgaaagaca gaaaaagtag
     2341 ctataacata taattctata ctataataca agttcaatat aaaatgataa aaagaggatg
     2401 tatcaatatt aagattaaat ttattatact ttgatatatc ctctttaata ttatcaactt
     2461 tgatttaaat atttttaaaa tagttgataa gaaagttttt gaacaacaac acagctttag
     2521 attcatagtt ttcgctaaac catgaaagat ttaaatatct tttaaattca atatctttga
     2581 tatgtaacaa cttaatctca gggtcttgag ataatcccca tgattttcca catataaatc
     2641 caactccttg aagtgatttt ataagagcat aaattgtata tggtgaatca gactcaaatg
     2701 ctattttggg tttgaaatta gctgattcgc acaggatatc aattacctct ctataattct
     2761 cacccttcgt tataactata aagttttcat ctgaaacttc gcttagatat acttcatcct
     2821 ttaatgataa aggatggttt attgaaactc ctaatagtat ttcttcgcat gttaaagtta
     2881 tgctattttc tgagtttagc tttgtaaatg aagaagatat gtacaaatca aaatcacttt
     2941 ttttataaga tgatggcaaa gtatgtgaca cattaaaagt aatattggga tatagttttc
     3001 tgaaattact caaaagtgat ggtaacacat tagccgctgc aggagataat atctttactt
     3061 ctcctgaatt ttccaaagaa gtgtcttgta actccttttt tgaactttct actaaatcta
     3121 aaatgctatc tactcttttt agaaacaatc ttccatactc atttaaaaca atatatcttc
     3181 cttttctgtc aaataactga actccaagtt ctttttctaa ggaagatata gttttgctta
     3241 aagctggctg tgctatattt aacttgtgtg atgcttttgt tatgtgctcc attctagcaa
     3301 cagtttgaaa atacttaaga tgcaataaat ccatagtata aaatacacct cttttaatat
     3361 gacttgagtt atatcaattt tataatcaac tccacattat acattataat ataaagttta
     3421 tataataaac tacaatatat taaaaagtta tttaaattgt tatttatatt ttgtacataa
     3481 tctaattaaa aaaagtaact ttaaatggtt aactaaggaa atattgttac aaactttagt
     3541 tatataaaaa attcgtttga tgagcaaagt attaatgtca agtaagaata aaagcttatt
     3601 agataagaaa aataatattt tataataatg atgttactta aattattatg ttatactatt
     3661 ttaaatagga aacatttaga tactataatt ttagggggag tcaagatgaa attaactaaa
     3721 tcaatatcat caatgctgtg cttatctcta gttttaagct tatcaccagt agcatatgca
     3781 aatgaaataa gtagaacaac agttgagaat actttagtag gtcaggatag atatcaaact
     3841 gctgttgaag taagtaaaaa aggatggagt agtgcaaatg aggctgtagt tgtaaatagt
     3901 gaatctattg tagatgcatt agctgtaaca ccatttgcta aattaaaaaa tgctcctata
     3961 ttattgacag aaaaaaataa tctaaattca caaactgaga aagaattaaa aagattagga
     4021 gttaaaaaag tttatataat tggtctatct agttctgtat caaatgatgt gcaaaaacag
     4081 ttagaatcta ataatttaac tactgataga gttggtggta gtgatagata ccaaacagct
     4141 ttgagtatag ctaaaaaaat agaaggatta aaagatatat cagaaatagc tgtagtaaat
     4201 gggtacacag gtcttgcaga tgcagttagt atagcttctg tagctgctac taatggaatg
     4261 gttatacttc cagtatctga tgcatctggt atatctagct ttaaagattt tatagaatct
     4321 aagaatataa ataaatcata cataataggc tctactaatg ctgtatcaga taaaataaaa
     4381 caaagtttac ctaattcaga aagaataggt ggagcagata gaaatgaaac taatggtaaa
     4441 gtaatagaaa agttttatac atctaataaa ttaaacaatg tatttgtggc taaggatgga
     4501 atgaaaaatc aaaatcaact tatagacgct ttagcagtag gagtactagc atcaaaagaa
     4561 aattctcctg tactaatcgt aggagaacaa ttaagtgcaa cacaaaagag tgtattttcg
     4621 aataaaaatg ctacctctat aactcaagtt ggtagtggag gaaatgaaaa tgcatttaaa
     4681 gaattaaaag atttacaggc aaatagtaac aataagagag taatgtatgt aactaataca
     4741 gataaggtaa atataagaaa tgatgctaca atagaagctt ctgttatagg atatctaaat
     4801 aatggtgatg aagtagaagt tttagatgtg ttaaaaactg gatgggtaga aataaaatac
     4861 aatgaaggta ttggatatgt tagtgggtct tatttgacaa ataataagcc tgataatagt
     4921 aatgaaaata taaaaataaa gtatgtaaaa gaaaaagatg gtctaaatgt taggaaagga
     4981 ccaagtacag aagatgaaaa aataggtcac ctttcatatg gaagcaaagt agaaacaata
     5041 gaaatgtttg ctactggatg ggtaaaaata aagtataatg gtggatatgg ttatgtaagt
     5101 aatgattact tatctgatac accagttatt taattataag taacaataaa aaaactgctt
     5161 taatttgtaa agcagttttt ttataaaaat ttaagtctaa taactatttc tattttgata
     5221 ccttaatcct gggatttatt attatggata ttatcaacat acaaacactc caaatccagt
     5281 gtctaattcc aaacttagat attaataaat tcagtcctgc tatggtaaat aagcagtatt
     5341 taattacaat taaaagtgta tttatagaaa gccccttcat agaaagtatc ctaaatccaa
     5401 taatagacat tctaagtcct ataaatgcca ataatatacc tgcaatatcc tgaattaagt
     5461 aatagtattc aaatttcata ttcaatcaac tcttctctga gcagtatcaa atatttctaa
     5521 ctcttcaatt tcatcatctg tgtatgtaag catagtaaaa ccagttacac cataaataaa
     5581 tgaaattata ggtgtaatcc aacttaagaa acaatatgga ataaattcta gtgggcttac
     5641 accaagagtc tgagcacaga aaacagcatt tgaattccat ggtattaatg gaccaccaag
     5701 tgtagctgta tcttctataa ttctagatag attttgtggt tttaatttaa attctttgta
     5761 tattggagac attagagttc cgacaaatac agaagtgaac atcattgatg aactaaatgc
     5821 atttgcaaag taacttacta taaaagtaga cccaaccaat ttagttcttg attttcctat
     5881 tttagttata agaggttgaa gcagtacttc taagaatcca gttttttgga gaataccagc
     5941 aataacgaaa actacaaata ctagtaaaac ggtttcagcc atactcatta ttccaccacg
     6001 atttaatagt ttgtcggcat agacaaatcc agtatctata gtaaatcctg atagcatgta
     6061 atttaaaaca tcacctattt tttctccttc ttgaaatact gctataataa gtcccatgat
     6121 agctgaactt agtatagata taatgggtgg cttttgtagc aataaaagta ataataaaaa
     6181 tattattgga atcattgcta ctaaaccaat atgaaagttt gaatttaata catccttgat
     6241 ttgatttatt tgaacatagt ctatagtatt atttgaatac ttaaatccaa ttactgtata
     6301 cagaactata catattacgt atgcaggtcc tgtggtataa aacatatgct tcatatgtgt
     6361 aattacatct gtcttacaaa ctgctgcagc taagtttgta gaatctgata gaggtgacat
     6421 cttatctcca aagaaagcac cagaaatgac tgcaccagct gttaaaccaa gaggaatacc
     6481 catactagta ccaatactca tcattgctat accagaagtt ccaagagtcc cccaagatgt
     6541 accagtagca acagaagtta atgaacatac gattaatgtt gtaagcagaa aatatttagg
     6601 ggttattatt gttagtccac tatatactac tgttggtatt gtaccagcag ctatccaagc
     6661 tgctattagt acaccaacag aaagtataat taagtttgat tgaaaggcat ctttaccaac
     6721 ttcaaatcca aaagcttcta tttcatcatt agtataacca agtttcatag ctgcaggaac
     6781 tactataagc catgaaagta aaaacattgt agttatagaa gcattaaata caactatacc
     6841 aactgatgtt agagctatta ttgcaagcat tactaacaag gaatatctaa atgaaggtat
     6901 ttttctttct gtattcattt tcatagtgca aattctcctt aaatattttt agttaattgc
     6961 attttttatt cttataagag cttcttcaag catacttctt ggacaagcta agtttattct
     7021 ttgataccca cttcctccaa tgccaaagct gtttccttga tttagagcaa ctttaccttt
     7081 ttgtacaagt atactttcta gttcctcatc acttaatcca agtgcactaa agtctaccca
     7141 taataaataa gtaccttctg gttttctaac tttaagtttt ggcatatttt catttatata
     7201 ttttatagca aaatctatat tagattctaa atattctaaa aatgattcta gccaagattc
     7261 accattatta tatgaagctt cagttgcaac taagctaaag caattatttc tctttatatc
     7321 aattctggta aaggcatcat ctaacaattt atagtctttt tcatcaggta gtattacata
     7381 tgatgattga agaccagcta tattaaaagt tttagttgga gccatacaag taattgtatt
     7441 tttttcaaat tctttagaaa tagatgccat aggaatatgc ttatgttttt ttaatataat
     7501 gtcactatgt atttcatcag atatgatttt tacattatgc ttaagacaaa tatctcctag
     7561 tttttttaat tcatcttttg tccatactct accaactgga ttatgaggat tacaaagtat
     7621 gaataatttt acatctttaa tcttgttttc aatgtcttca taatccatta tgtagtttcc
     7681 attttctagt ttttgtaatg ggcttataat tagttctcta ttgttatttt ttacaacact
     7741 attaaaaggg ctgtaaactg gttcttgtat cattatttta tcatttgctt ttgtgagttc
     7801 atttattaaa agacttatag ctgggataac acctggacta taaattaacc actcactttt
     7861 tattttccag ttatgtctcc tatctagcca gtttactata gattcattgt aagaatctgg
     7921 tcttgttgtg tagccataaa tctcttgttc tagtctattt tttaaggaat caattataca
     7981 aggtgcagcc ttaaaatcca tgtcagccac ccacatagga agtaaatcat tagttccata
     8041 ctttttttcc atttctgacc atttagatga aaaattattg cttctatcaa caatctcatt
     8101 aaaattataa ttcattgtaa atacctccag taattttaat tttatgttaa taatattaag
     8161 caaacaacgt gccaatattg ataataaaaa atatttattc acaagttaat atttaatatt
     8221 aagtaatttc agagcataga taagataaaa atattaatta tacttaaaac tttgtggaaa
     8281 aggttatttc taataaattt ttaaatatat gaaaatgtaa ttaaataggt taaataggtt
     8341 taactgttta acttgttaga cctatttata ttttttagaa attctactcc ttttgatgtt
     8401 ataattgaac ctccacgacc aacaagtatg tctacaagcc caaagttttg tagttctaaa
     8461 agcatatttc taatctgttg ttctgtcaaa aatctatttt cctctaaagc tattttacat
     8521 aagctttttc tacctattct ttcttttaaa tcataagcct tttttaaatt atccaatata
     8581 aatatgtatt catctagtga ccttttaaaa ttatagtcgt atttcatgat gtttttgtct
     8641 ttgccaatgt tgaattgata cttttttata ttatcactta ctttattaca aacggttcta
     8701 ctataatttg aatctatagt atctaatata tattcaggta aatcacatat ttctataata
     8761 tctttttcca agtaacagaa gtattctcct aagtttctta attctcttac atttccttcc
     8821 catctataca ttttaaagat ttcttttact tcctcactta gaataaaatt acatggaata
     8881 tcatatttta atgaaccaaa aatttcaaat atatcagcag gtctttccct aagtggatgt
     8941 attttaaggg gaagaacatt caacctaaaa tataaatctc ttctaaacat atttttagat
     9001 acaagttctt ttaaatttct attagtagct gcaattattc ttacatcaat gtcaatgaca
     9061 ttattagaac caatacgtcg tacttgtttt tcttgtatga ctctaagtaa tttagattga
     9121 gaatttaaat ccatttctcc aatttcgtct aaaaatatag ttccattgtt ggctaattca
     9181 aataaaccaa ttttcccacc ttttttagca ccagtaaaag ccccttcatc atagccaaat
     9241 aattcacttt gcagtagatt ttcttgaaaa gtactacaat ttactgcgac aaaaggacca
     9301 tcttttctcc tagaagcact atgaatggat tgagcaaata attctttacc agtaccactt
     9361 tcaccaatta taaggataga tgaatttgat tgagccattt tattagcaat tttttttgta
     9421 ttaatagttt gtatactaga acctaatatg tcatcaaatg tgtatttact gatattacca
     9481 gagtttacta gttgtgctct taatttagct tgcttttttt ctaattgaga aaactttgtc
     9541 atctttagta taaatccatc aaatacattt aattgtatat gtttaatctc taaatttata
     9601 tcaatatgat ttattttaat caatttctca aaaaatggag ttttattggt caatatgctt
     9661 tgaaaattga tgtcttttat gcaatcacca acaaatgagt caatcatttc atttgcattt
     9721 atagacaaaa tttttctagc catatgatta tagaattgaa ttattccatc gttatttgtg
     9781 catataattc catcatcaat tatagatagc aacaaatcaa attgtctttc aaacttattt
     9841 gcatctaaca ataactcacc tgtactataa cttgtaggaa ttattttttc tacgtatttt
     9901 tttattaggt catcttttat tagatgtttt aatttggttt ttgtagcaat ttcaacaata
     9961 caacttaaat ctattattct gtattttaaa tctataactt cacaattttt aaacttaggc
    10021 aagatttttt cgccaggagt taggattaca gcatctgtgg gagtaaactc aatctctgga
    10081 tagcagggca ggatatttat attgtctatt cctaatctat gtattaaaga aattgtttct
    10141 attgccatat ctttacttaa gttatataca taaataattt tatctttttc taatttggat
    10201 atcatatcaa tactgttctt ttcaaatgtc aacttaggaa caatgatttg agcatcatag
    10261 tgagataatt ttactatttc atcatatttt aaataagcag ataggagtat aagtttttct
    10321 ttaaaaaact ttaaatttcc ctcttcaaat gagtataaat ttatctttag attatcacct
    10381 aagaattgcc ttatttgatt ttcataaatt tctccagcgt ctttttttaa acttacaata
    10441 gctaattcca tttgtttcat tttaacctct catacaaatt tgacttattt tagattacat
    10501 aataattaaa ttagattaaa taatattatt taattattat tataaattta ttatagacta
    10561 cacattgaag taaaagaagt agctaaaatt attcctttta tttttgtttt ttgtatctat
    10621 ttggtataat agtattagag cgtataataa tttaggcaaa attttttaag gaagactaga
    10681 ggagaatata aaatgaaaaa atgtctttac tgtaataggg atataaagtc accaaataat
    10741 aaattagcta taaaatatga tgacaatatg aatatatatc catgtcgtgc agaatgtaag
    10801 gaaaaaattg aagaatatat agcaaaaaaa cctacatata agtttataaa tatattagag
    10861 gttcttttag gtgtgggcgg aatattctgc tttttgagta aatggactat tgcagcccaa
    10921 gttgtacttg gaattgatgc actggttata tttttattcc cattggcagg atttaaaatg
    10981 aatggaaagt taagtatgga acaaactaag aatcaaagta ggtctattgc aatatcaatt
    11041 ttggcattta tagtaataat aactgtatta tattttaaac aagttggtaa gtagagcata
    11101 attgtataag acatcattag agtatgaggt gagtttatgg aagaaagaaa attagcacca
    11161 aacaatgaga gggaagctct cataaaaaga ttaaaaagaa tagaaggtca agttaagggt
    11221 atacaaaaga tggtggaaca agaacgttat tgtgttgata tattggttca aatatctgct
    11281 ataaggtctg ctataaatag agtaggcaca attatcttag agaatcatat aaaaggatgt
    11341 gtagcagaaa gtattaaaca tgatgatgct gataaaactg aagagatgat aaaagagctt
    11401 atggatacta taaataaatt tacaaaataa ggaggcggtt agttatgaat caacaaggat
    11461 ttattatgga aattgttgat gatagaactg caaagttaaa aatgcaaaga cattcagctt
    11521 gtgctgcttg tggaaaatgt gctactacaa attcagcaga gtcaaaagaa atattagtag
    11581 aagtagacaa cacaattggt gcaaaagttg gtgaccatgt agaagtgagt atggataata
    11641 tgaatgtatt aaaagcagct gtaatggctt atataatacc tcttatagca cttcttgtag
    11701 gaacaatagg gacttattat atattggggg caattggaat gacaactgga gttgaagcca
    11761 taagtggatt agttggaatt tgtcttacaa taatctgtta tttatactta aagaagaatg
    11821 acaaaaaatt tagagacagt agagaattta taccagtaat aacaagaata ttaattgacc
    11881 tttaagataa ttataattaa aaaatagcaa tgtacttttt aaataaatac tcaatacgta
    11941 tttgtagagt ttaagtacat tgctattttt atatttttat tttgattttc tgtaaggaat
    12001 aaagtttttt ctgattttta aaagattcaa tgttcttaca aaagaagtta caactaatac
    12061 accaagtgct attgcagcac ttagaaatag cgcatctgta ccttttgaca tagccaaaga
    12121 ataatttttc tgaatcaaag acaacattgt attatatagc ccaattcctg gtattagagg
    12181 aagaatacct ggaattacga acacaatagc tggttgcttt agttttcttg ctaaagtctc
    12241 actgcacgct gagaccaaag cagcagcgat aaaacctgca aaaactgcat ttgtagtaaa
    12301 attaaataaa taaacatata caatccaacc aattccgcca gttactccag ctggaagaag
    12361 tagatatagt ggagcattaa agaaaacagc aaatccagca gttgcaacac ctgaaaataa
    12421 aaaatgcaaa atcatagaca tatcagtcat tagaaaggac ctcctattct ataccatgta
    12481 tcaaggacaa aaccaactcc agaagcaatt gcaataataa tcatagcagc atcaaaagca
    12541 cgagaaattc ctgatattaa attccctgat attaaatctc taatagcttt tataaaagca
    12601 actcctggga gtagtggcat tatagcgccg actataagca ttttagggtc atctaaaatg
    12661 cctatttcaa ctagtagaac tcctgaaagt gcaattaaaa aagaagatac taaacaggaa
    12721 aatgcagata ttgcacttat cttcatcgtt ttatcataag taattgcagc aaagattgct
    12781 attataaacg taagtataaa atttaataca taattccctc caagcaaaca agcaaaacaa
    12841 gcagatgcaa ttcctgtaca agagtaaaat aaccataaag gataaggttt tacatcttgg
    12901 atttcataaa gacgtgcaat ggcattatca atatcatact ctttgtcact aacaaattct
    12961 cgagaaaaac tgtttaataa agaaatctta tttaagttta tacctcttga ttgaatcgtt
    13021 ttcataaacg aaaagccatc aaatttctca tctgaaataa ttattactgt aggagttgta
    13081 aaaatattta catgataaaa ccctcttgac ctgcatattc taaggacact gtcttcaacc
    13141 ctagatgttt cagctccatt ttttatcatc agttccccta caaatagagc tagttttaga
    13201 atatttttct tatattcaag ttcctcattt tgattcataa atacacctcc aaattaaaaa
    13261 acgtaatatt gaaattatat caaattttta agtaatattc cttaatatct atacaaatat
    13321 attatctttt ttttgtttca aaatagtcac atttaaatta tatccaatta aaagaattag
    13381 acaatattat ttattatatt ttaacttaaa aataaagaaa gtaactatgt taaatatgga
    13441 aaaatggcaa aacacttgac tattaattgg taaaggacta taataataaa ttatcatatt
    13501 gataaaaacg aaaattatga aatgtctgta aaaataaaat ttaaataata actaggagga
    13561 gtaatatgtt tgagaacttg aaaaaagcgg accctgagat ttatgaaagt atgaaaagag
    13621 agttaaaaag acaacaaaga aatattgaat taatagcatc agaaaacata gtatcagttc
    13681 cagtaatgga aactatggga agtcatctta caaataagta tgcagaaggt tactcaggca
    13741 aaagatatta tggtggatgt gaatacatag atgaagttga aactcttgca atagatagaa
    13801 tcaaaaaaat atttggagca gaacatgcta atgttcaacc tcactctgga gcaaatgcaa
    13861 atattggtgt atattttgct atgttaaaac caggtgatgt tgttatggga atgaatttat
    13921 ctcaaggtgg acatttaact catggtgctc cagttaatat ttctggtcaa tattataaat
    13981 tttatgaata tggtatagat aaagacagtg gacttataga ttttgatgaa gttagaaaga
    14041 tagcacatga agtaaaacct aaaatgatag ttgcaggagc aagtgcatat cctagagaaa
    14101 tagattttaa aaagtttaga gagatagctg atgaggttgg agcattgctt atggtagata
    14161 tggctcatat agcaggtctt gtggctgctg gtcttcacca aaatccatgt gaagtggctg
    14221 attttgttac aacaacaact cacaaaactt taagaggacc tcgagggggt gttattcttt
    14281 gtaaagaaaa atatgctaaa gacatagaca aagcaatatt ccctggtatt caaggtggac
    14341 ctcttgaaca tataattgct tctaaggcag tttgctttaa agaagcttta agtgatgagt
    14401 ttaaagaata tcaggtacaa gttgctaaaa atgcaaaagc cttagctgaa gaacttatta
    14461 aaagagattt taaattgatt tctggaggta cagataatca tttaatacta ttagatttga
    14521 ctaataaaaa tataactggt aaagcagcag aaaagagatt ggatgatgct tatataacag
    14581 ctaataaaaa tacaattcca tttgacccaa atggtgctct tattacaagt ggaataagac
    14641 ttggtactcc agctgtaact actagaggta tgaaagaaga agatatggca ataatcgctg
    14701 aggctataga cctatgttta acttatgatg aagaatctaa ggctagaaca ttggttgttg
    14761 gtttaactga aaaatatcct ttatatgaag aatataatat tatggattaa ttataaagaa
    14821 caactaaata aaatacattt aaataattac aatgattgtg tatgtatttg aatatagtta
    14881 gatataaaaa aggtgtctca aaatgaactt tttagtttat gaggcacttt tttgtatgga
    14941 atcgaacata tttgtgttat ttaaataata aaaaatatgg ttatctctat ttttagatgg
    15001 tctctttgta ttttttaatt aaaactttaa gatttcttaa gaattatact aggtttttct
    15061 aaagatttaa agtctatact tataagtata gtaatcatat gaaaagaaaa gctttaacga
    15121 ggagggaatc ttatgaaggt attaattctt acaggaaaat ttggtatggg tcattactca
    15181 gcatcaaatt ctttaagtga agatataaaa gcaaaatttg ataattcaga aataataatt
    15241 aaagatatat ttgagtacat tatgcctaac tattcagata aaatgtacaa gactttttct
    15301 attcttgtaa atcggggaag tagtctttat aatttatttt ataaatgtgc tgaaaatgga
    15361 aagaaagata taaaatttac tttttcagat tattttttaa ataaattaga tacattactt
    15421 catgaagtac aaccaacagt agttatttca acttttccat tttgttctca attagtatca
    15481 agatataagg aaaaatataa ctcaaatctt cctttaataa catgtataac tgatataagt
    15541 agtcactcag aatggattag taaaaataca gactgttatt tagtagctag taaatctact
    15601 aaggaagaac ttgtttttaa aggaatagat gaatctaaga ttaaagttaa tggtattcca
    15661 gtaaaaaaag aatttaaaag aatagaacat gtaaatcatt caactaaaaa aaatattctt
    15721 ataatgggtg gaggattagg attactgcct aaatcagaac aattttacaa agaattaaat
    15781 agtttagaag gagtaaaaac tactgtaata actggtaata ataaaaaaat gtactataaa
    15841 ctatatggta gatatgaaaa tatagaggta gtaggttaca ccaatgaagt atataaatat
    15901 atgaaagatt ctgaccttat tatttctaaa ccaggtggaa taacactatt tgaaacaatt
    15961 tattcagaac ttccaatatt ggcatttaat ccatttttac aacaagagat agataatgct
    16021 agttttatat taaataatga aattgggaga atacttggta aaaataaaaa atactatgta
    16081 gatgagataa aagatttaat ttatgatgat gctactctta aagaaatgag cagtaacatg
    16141 aaagaattaa aaaaacaatt tgataataat accttagaga atattttatt ttcacttgat
    16201 gaacaaggag cgtgtagaga atgtatgtag taggtttaat tatcgttgtt gcattgattt
    16261 ttttagtcca ttcaattata cctacatact ataataaact attaaataaa gaggtattaa
    16321 aaaatatggc tggagaaaat gaaattgcac ttacatttga tgatggtcca gataagagat
    16381 atacagaaaa attactagat gtgttaaaag aaaatgatat acaagctatg ttttttgtag
    16441 tagcaaagaa tgctgaaaaa gagcctgaaa ttataaaaag aatgttgaga gaaaatcata
    16501 tagttggttt acattcattg gaacacagaa atgcatggct ttatagttat agttatgtta
    16561 aaaaagattt tattgaaagt acaaatataa tgaaaaattt aggtgtagat gtaaactact
    16621 atcgtccacc atggggtcat actaatatat tttcaaattc atttgttaaa aaatataatc
    16681 taaaaatgac tttgtgggat gtaatggcag aagattggga gaaagatagt acagtagaca
    16741 ttattataaa caagttaatg agtagaacaa aagaaaattc tataatttgt ttacatgatg
    16801 caggtgaaaa ttcaggtggt gcagtaggtg cacctgagcg tactattgaa gctttaaaga
    16861 tagctattcc aaaacttaaa gccagtggtt taaagttcgt aacaccagaa aggatgtagt
    16921 tatgataagt aaaactgaaa agaaaaataa atgcctaggt agtgtagctt ttttagtttt
    16981 actaatgggc ataacaggat attttgtttt tagagggcag tcagttgaat cattgataaa
    17041 atcattaaaa ggtgcaagcc ctatgtttat actaattgga tttgcaatga tgtttatata
    17101 tgttgcctgt gaaggtataa atatatactt aggtatgaaa gctttaaacc aaaagactac
    17161 tcttttaaaa tgtatgggat atgcttttat tggattttat tttagttcta ttacaccttc
    17221 agcatcaggt ggtcaaccag cacaagttta ttatatgaaa aaagacgata taaatatatc
    17281 atattcttca cttatactat tagtcattgt tgttattcac caagtagtta tactggcata
    17341 tagtggaata atgttcatta tggaacgtga gtttatatta aataatgtaa gtggtatgaa
    17401 cattttacta atatatggtg taataacaaa tgttgctctt gtcattggag taattgcaat
    17461 tatattttca aaaaaacttg taaacaattt tataatatca ataacaaatt tattaggtaa
    17521 gttaagaata ataaaagatg ttgaaagttc aagaaaagtt ataatatctc aaattgaaga
    17581 gtatgttaaa ggtgctcaat atataaagca gaatccaaaa cttgtagtcc aaattttagt
    17641 cataactatt gtacaaataa cagctatgtt tttagttcct ttctttgtat acaaagcttt
    17701 tcatctatca acatatactg tatttgagat attagcgata caatccttgc ttaatattgc
    17761 tgtttcttca ttaccattgc caggggctgt tggagcttct gaaaatagtt ttatgacatt
    17821 atttaagata tttttccctg gtcacttatt agttccggca atgttactta gtaggggaat
    17881 aagtttttat gcatttgtag ctataagtgg attgatatgt atagtagtac atgtaaaatc
    17941 ttctgaaaaa gtaaaagcag tgaaaaaagt tgcttatgta agataatatt atatagatta
    18001 tttttattaa tatgtcaatt tgtgtgtata ttttaattaa gctttaaaca tatttacagg
    18061 agtagaaact attaaaacgt gtgacattgc taagcttttg ctaataaagt ataatatttt
    18121 atatagtttg aaatatttaa gaaatatagc aaaaaaagga gcttggtgat gaataaagag
    18181 attccaaaaa aaattttagt agctgatgat aatcctgaga ttagggaaat cgtagaaata
    18241 cttttaactg gtgaaggtta tgaagttatt atggcaacga atggagaaga agctgtaaac
    18301 ttagtagata gtacgattga tttgattatt ttggatgttg taatgcctgt aaaaacagga
    18361 tttgttgcat gtaatgaaat tagagaaaaa acgacagcac caatattatt tttaactgca
    18421 aaaactcaag actctgataa agttattgga ttttcagcag gaggagatga ctatttatca
    18481 aagccttttt catattctga actaatttca agagttaagt ctctacttag acgttattat
    18541 atataccagg gaaaggaaaa ggaaagagag ccaaatatat taaaaacaaa agagttatgt
    18601 gtcaatttag atactcaaga tgttacacta aatggagaac ctgtgttttt gacaactata
    18661 gaatatagta tattaattct tatgttgaaa aatagaaaaa aagttttttc gtctgaaaat
    18721 ttatatgaaa ctatttggga tgagcagtat ttttatacag caaataatac aataatggtt
    18781 catattagaa atttaagaaa aaagttagaa aaagaccaaa aaaatccaca atatataaaa
    18841 accgcatggg gaaaggggta ttatattgat taacaaaatt aagccatcta agttgggagt
    18901 tatactattg atatatgtag taatctcagc tgcagtggct ttcattttaa atataccatt
    18961 ttactctgct gttgaatcta tagtaaaaga tgtatcagaa gggaaagtat attttaaaag
    19021 agaagtaaat caacttatgg ataattttca aacttatgtg aataacaatg gtataagagc
    19081 agatgatgga gatgatatag caaaatggaa tagtgataat tggtttgttt ctatgaagat
    19141 atataaagga aataaggttt tttatgatac attatactat cctaatgaag tacctgataa
    19201 gagtgaggaa aatgagtttt atagcagtcc catggctaga aactatgaga tacaattttc
    19261 tgatattaaa gctaatatag ttgtagtagc tttatttaga tttagatttc aaaattttat
    19321 agaagcaatt tctattacat ttatggcact tacatttatt ataacatttt tactgttgtt
    19381 tagtagaaag ataaagtacc taaggaaaat agaaatgggt ataaagatag tagagagtgg
    19441 cagtttaaag tatagaatac cagtaaaagg taatgatgaa ttatcatcac ttgcatctag
    19501 tattaatgag atgagtcata cattggagaa tcaacttata aaagaaaggg agatgaagaa
    19561 taaaaataaa gatatagtaa cttcaatatc acatgatgtt agaactccat taacgtcagt
    19621 aatttgttat ttagatttga ttaaagacca taaatacaat agtcctgagc aattggaaca
    19681 atacataaaa aatgtaaggt ctaaagcata tcaaataaaa gatttaacag atgacctttt
    19741 tacgcatttt gttaattctg gagaggaagt ttcaaaaggg tatgaaaaaa taaatgctaa
    19801 tgaatttatt tttcaattac ttttagatag ttcatataca ttggaggaga aaggatttca
    19861 tataacaata gaaaatgaac tttataacca atatcatatt tatgtagatg ttacacaatt
    19921 tagaaggata tttgataatt tatgttctaa tataataaaa tatgctagga aagataagcc
    19981 tatagtattt ttaattaaag tagaggattc taaattacta attatacaaa aaaatagagt
    20041 aagtaatttt tctaagaatg tagaaagttt tggaattggt ataaagaatt gtgagaaaat
    20101 agttgcatat cataatggaa gcataaatgt aaattcagat gataaagaat ttgctattga
    20161 aatatctatt ccagcaaaag aatcataaaa atttaaatta tatattgtgt aattgctatt
    20221 atatattgtg taattgctta tgtaaaattc tatgaagcag gaagcatgtg actttagtta
    20281 tgggatgttc aatattgtgt acctctaggc aagtaacaag tttacttaca agaccatata
    20341 atattattat cttaaaaaaa ggggacataa taatatggtt ttgacaggag cacattatat
    20401 ttacattcta tttatggtaa ttatattaat tactatgatt atgaaaaagg atacaatagt
    20461 accatgtata ttaggtgttt tttttatggg gttgttcttt gaaaaaaaca tttttggtgc
    20521 tataacagca gtgtttaact cttttattat atctttaaat gagttgggtc caattatctt
    20581 gattatagct attatggttg cattatctaa agccctagaa gccaataatg ctatacagta
    20641 tatggtgaga ccattttcga gagttataaa aaattctaat acagcatttt ttgtaactgg
    20701 atttgttatg ttagttcttt cttggttttt ctggccaact ccagctgttg ccttagttgg
    20761 agctgtattt ttacctgttg ctatgagagc tgggctacca gcaattggtg ttgcagtagc
    20821 attaaattta tttggtcatg gtcttgcctt gtctacagac tttgttattc aaggagctcc
    20881 aagtataaca gctggtgcag caggtgttgc agtatcatat gttataaatg atggtatgat
    20941 tttgttttgg gtgatgggaa tcgtaaccat atctatggca ttttatacat taaaaagaga
    21001 tattaacaaa ggaatgttta gagaagaact aaaaggtttt gaaagtgaag aagtagaaga
    21061 gttcaatgga aaatctaaaa tagcaactat tttagtagct ctgggatttc ttgctgatat
    21121 aattgctatg tatatatttg acctaaaagg aggagatgca tcagctcttt tgggtggaac
    21181 agcagtattt ttaataataa ttataaatgt tataaatttt ggcaaagata gtcttgaaaa
    21241 tgtatgtgag aatataattg atggttttgt gtttgggata aaaatatttg gagcaattat
    21301 accaatagca gcatttttct atatgggtga agttgcacca ttgacaggtg tatttggaaa
    21361 agtattagca ccaggttcac aaggtttatt atcagatata ggcatagctc tttctcaaac
    21421 aataccactt aataaatttg ccgtatcagg tatagaaact gtagtgggag caataactgg
    21481 gcttgatggt tctggatttt ctgggatttc attagttggt tcacttgcct ctgtatttgg
    21541 aactgctata aatgcaagtg ttggtgcact agctgcatta ggacaaatca gtggtatatg
    21601 ggtaggtgga ggctgtctag ttccttgggg tcttatttca gcagcggcaa tttgtggagt
    21661 tagtccaata gaattggcaa agcgaaactt tgtcccagtt ataaccggat tggttgtaac
    21721 aaccatagta gcaatgttta ttatttaata aatcgaataa atctttaatt gtataaatta
    21781 aaaatagatg tgtagctata atttattata attgttttac taattctaag ccatacatct
    21841 atttttattg atatttttat tagtattctt taataaaata atatttttca ttatactata
    21901 cataacatct aaatataaca tctaaaatac tgttatctat atttaatatc ttgccaaatt
    21961 ttttgatgtt ttattgccct gtcccaccaa catatataag ctatcacaag tttttaccta
    22021 tactatcacc aaacaaaaat tctaatttta atttattatt ttataaccta tattatatta
    22081 actatattag tttgtagact ttagatgtaa ttataaatat taatactcca agagttcaaa
    22141 ttcatatcct agttttcttg catttgatat aaaatcattt aaaatttctg tactagtttt
    22201 ggaaactgca tgtagtaaca agatagaacc atcatgtaga ttatccatta ttttttgcgt
    22261 tgcttcttca tgtgatggtt gtttatcagt atcccagtct ctgtatgcaa aactccaaaa
    22321 tacagtcttg tatcctaaat ttttagtcat tgccaagctt ttttctgagt attttcccat
    22381 aggaggtcta aaaaatttaa ccatatcttt cccagttaca tctttatata acttttcaac
    22441 atcatataat tcgtcattaa agttttttag attgcttgtt tttgttggca tagatggatg
    22501 ggttttacta tgatttccaa ctatatgtcc ctcagaaacc attcttttta ctaaatcttt
    22561 attttctttt atatatggag ctgtaacaaa aaaaactgct ttaacctgat tttgttttag
    22621 agtgtcgagt atttttgtag tgtatccatt ttcataacct tcatcaaatg taagatatag
    22681 tgttttttga ccatcttttg taggtccatt gtatagggca tcatagtcag aaaatttaaa
    22741 ttcaatgtcc gtatttactt ctggtgtttt atgcttattg tttggtatat aaaaccaatc
    22801 aagagtagtg ttatctaaag aagatacatc taatttatta ttatcattat tagtagtggc
    22861 ttttgatggt ttatctgttc cgtctgaaac tattacattg gaagtatctt taccactatc
    22921 tattttttcc ttatcttctt gtaactgtgt ttccttattt tgattttcgt tttgattgtt
    22981 ttgtgaattt gaacatccaa caataaaaaa aatagaaata gaaagtagtg ttaaataatg
    23041 caatactttt ctaaacattt tgaagctcct ttctaaatta ataataattg catataataa
    23101 taattatatg attaattaca attgcatata gtattattat aattggaaat tactattaat
    23161 aatagttata tctttaaaaa taaggtcaca atagtatgac aaagggtgaa taatcaataa
    23221 atttaaaaaa aaatgaaaaa aatagttgac catattatga gaagatgtta ttataataaa
    23281 tgtgctcaac aagaagtaac aaaataacaa gcacaaagaa gttaattgaa t
//
LOCUS       DAMJZE010000043        23116 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJZE010000043 DAMJZE010000000
VERSION     DAMJZE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.043"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            107..1393
                     /locus_tag="PC541_001545"
     CDS             107..1393
                     /locus_tag="PC541_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2839013.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1505..1978
                     /locus_tag="PC541_001546"
     CDS             1505..1978
                     /locus_tag="PC541_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1637..1639,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2839014.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2010..3320
                     /gene="grdB"
                     /locus_tag="PC541_001547"
     CDS             2010..3320
                     /gene="grdB"
                     /locus_tag="PC541_001547"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3057..3059,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2839015.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3576..5108
                     /locus_tag="PC541_001548"
     CDS             3576..5108
                     /locus_tag="PC541_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2839016.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5119..6264
                     /locus_tag="PC541_001549"
     CDS             5119..6264
                     /locus_tag="PC541_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2839017.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6574..7653
                     /locus_tag="PC541_001550"
     CDS             6574..7653
                     /locus_tag="PC541_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2839018.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7766..8365
                     /locus_tag="PC541_001551"
     CDS             7766..8365
                     /locus_tag="PC541_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839019.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8551..9423)
                     /locus_tag="PC541_001552"
     CDS             complement(8551..9423)
                     /locus_tag="PC541_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2839020.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9840..11243
                     /locus_tag="PC541_001553"
     CDS             9840..11243
                     /locus_tag="PC541_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839021.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11270..12814
                     /locus_tag="PC541_001554"
     CDS             11270..12814
                     /locus_tag="PC541_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2839022.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12845..14236
                     /locus_tag="PC541_001555"
     CDS             12845..14236
                     /locus_tag="PC541_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2839023.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14429..15925
                     /gene="abfD"
                     /locus_tag="PC541_001556"
     CDS             14429..15925
                     /gene="abfD"
                     /locus_tag="PC541_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2839024.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16060..16335
                     /locus_tag="PC541_001557"
     CDS             16060..16335
                     /locus_tag="PC541_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839025.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16359..17666
                     /locus_tag="PC541_001558"
     CDS             16359..17666
                     /locus_tag="PC541_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2839026.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            17686..18804
                     /locus_tag="PC541_001559"
     CDS             17686..18804
                     /locus_tag="PC541_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2839027.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19091..20011
                     /locus_tag="PC541_001560"
     CDS             19091..20011
                     /locus_tag="PC541_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2839028.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20126..21283
                     /locus_tag="PC541_001561"
     CDS             20126..21283
                     /locus_tag="PC541_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2839029.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21368..22867)
                     /locus_tag="PC541_001562"
     CDS             complement(21368..22867)
                     /locus_tag="PC541_001562"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2839030.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         8902 a         2731 c         4370 g         7113 t
ORIGIN      
        1 aaacacaata atatatcttt aaaaaaataa gataaattgt aaaatgatat aattttgaga
       61 aaaactcaaa tgcatataca caaattaaat aacaggaggt gcaagtatgc gtcttgaatt
      121 agggaaaatc tttataaaag atattcaatt tggtgatgta acagaagtaa aagatggagt
      181 attattcatc aataagcaag agatgctaca agaaattggt ggagatgagc atattaaatc
      241 tatagatata gaattagctc gtccaggtga aagcgtaaga ataacacctg taaaagatgt
      301 tattgagcca agagtaaaag ttgaaggtaa tgggggaatc ttccctggta tcatgtctaa
      361 agttgacact gtaggagaag gaaaaactca tgctttaaaa ggtgtagctg ttgtaactac
      421 tggaaaaata gtaggtttcc aagaaggtat aatagacatg actggagaag gagctaagta
      481 cactccattt tctaaattaa ataatgtagt agtaatagct gaacctattg atggtttaaa
      541 acaatacgct catgaaaaag ctgttagaat gataggattc aaagcagcaa tgtacttagg
      601 agaagtagca agaaatctta ctccagatga agtttcagta tatgaaacta aacctcttct
      661 tgaatcaata aaagaatatc cagagcttcc aaaagtagga tatgtttaca tgcttcagac
      721 tcaaggttta ttacatgata cttatgtata tggagtagat gcaaaacaaa tagtaccaac
      781 tttattatat ccaacagaat taatggacgg agcaattgta agtggtaact gtgtttctgc
      841 atgtgacaaa aacccaagtt atgttcatat aaacaatggg gttgttgaag acttatatgc
      901 aagacatggt aaagatataa actttgtagg tgtaattata actaatgaaa atgtttatct
      961 agcagataaa gaaagatctt caaactggac ttctaaatta tgtaaatatt taggattaga
     1021 tgcagttata gtttcacaag aaggttttgg aaatcctgat actgacctta taatgaactg
     1081 taagaagata gaaatgcaag gtgttaaaac agttatagtt acagatgaat atgctggacg
     1141 tgatggaggt tctcaatcac ttgcagatgc agatgtaaga gctgatgctg ttgtaacagg
     1201 tggaaatgcc aaccaagttg tagttcttcc taagttagat aaagttattg gacatttaga
     1261 agtagtagat gtaatagctg gtggttctga tggaagttta agagctgatg gaactattga
     1321 agttgaaatc caagctataa caggtgcaac taatgaaaca ggatttggtc atttaactgc
     1381 taaaggatat taagaagtat tttattaaac gattgagtaa tgttaataaa acatattata
     1441 gataattata ataagtgtta tagatatata aaaatatatt agaaatataa taaggaggat
     1501 tattatgagt ttacttagta ataaaaaggt tcttataata ggtgaccgtg atggtatacc
     1561 aggacctgcg atagaagaat gtgtaaaaac agtagaagga gcagaggttg ttttctcatc
     1621 tacagaatgc tttgtctgaa cagctgctgg ggctatggac ttagaaaatc aaaacagagt
     1681 taaagatgct gctgataaat tcggagctga aaatgttgtg attttactag gtgctgctga
     1741 agccgaagct gcaggtcttg cagccgaaac agtaactgct ggagatccaa ctttcgctgg
     1801 accacttgct ggagttgcct taggattaag tgtttaccac gttgttgagg aaccaataaa
     1861 atcattattt gatgaaagtg tatatgagga ccaaataagt atgatggaaa tggttttaga
     1921 agttgaagaa atagaagaag aaatgtctgg tataagagaa gaattttgta aattttaatg
     1981 cctaaaagca aaaggggtga aactaagaga tgggaaaact aaaagcagta cattatatta
     2041 atcagttctt tgctggtata ggtggagaag aaaaagctga tactaagcca catgtggcag
     2101 aaactttacc tccaataagt ttacaacttg ataaattatt aggtgaagat attgaaatag
     2161 ttggaacagt agtttgtgga gatagctatt tcaatgaaaa tatagatagt gcaagtgaag
     2221 aagttttatc aatggtaaaa ggttttgaac cacaactttt catagctggt cctgcattta
     2281 atgctggtag atatggggtt gctgctggaa caataactaa agttgtaaaa gatgctttga
     2341 atataccagc tttaacaggt atgtatgttg aaaatccagg agcagatatg ttcaaaaagg
     2401 atgtttatgt agtagaaact tctgattctg cagcaggtat gagaaaggca cttcctaaga
     2461 tagctaaact tgcagtaaaa cttgctaatg gagaagaaat tggaactcct aaagatgaag
     2521 gatacatagc tagaggaata agagttaact acttccatga agacagaggt tctaagagag
     2581 ctgttgacat gttagttaag aaaataaaag gtgaaccatt tgaaactgaa tacccaatgc
     2641 caaactttga tagagttgac ccaagtaaag ctgtaaaaga tttatcaaaa tgtaaaatag
     2701 ctttagttac ttctggtggt atagttccaa aaggaaatcc agatagaata gagtcttctt
     2761 cagcttctaa atatggaact tattctatag caggagttat ggatttaaca gaagagactt
     2821 atgaaactgc acatggtgga tatgacccag tgtatgcaaa tttagatgca gatagagtat
     2881 tacctgttga tgttcttaga gatttagaaa aagaaggtgt aattggaaaa ttacatgaga
     2941 ctttctatac aactgtaggt aatggtacat ctgttgcaaa ctctaaaaaa tatgcttctg
     3001 aaattggagc agcattagtg gctgatggtg tagatgcagt aatattaact tctacatgag
     3061 gtacttgtac tcgttgcggt gcaacgatgg taaaagaaat agaaaaaact ggacttccag
     3121 tagttcatat gtgtacagta gtacctattt cattaacagt aggggctaat agaatagttc
     3181 caactatagc aatacctcat ccacttggaa atccagcgct tgaccctaca gaagaaaaag
     3241 ccctaagaag aggccttgta gaaaaagcat taaatgcttt aactacagaa gtagatggtc
     3301 aaacagtatt tgaaaaataa aaataacaca cacatatatt atatagataa aaatataaac
     3361 acacactaaa aataaaaaat aactatacac aaaaaaataa tatagatttc atgaatggat
     3421 aaattggagt tattcattca tgaaatctta aaaaatgcca aaatacataa acaataatag
     3481 aatcaaatat acaataaaat acaatgatta gagcttttaa taagtttaat aagattaaaa
     3541 aataagtgtt cataaaattt atggaggtgc acaaaatgac ttatccagtt ttaaaagggg
     3601 caggctatgt acttattcat actccagata tgatagtgca aaatggaagt acttgtacag
     3661 ttgaaagagc aacaaatcca gattcagaat ttttaaaaga agtaagtaat catataagaa
     3721 gttatgaaga tgtagttaac tacatgccaa atcaagttta tataggaaat agaagaccag
     3781 aagaattaag agatttacca atgccatggt gtgaacaaaa gattgaggga actagaaatg
     3841 gtaagtttgg agaaataatg cctcaagatg agttcatagc tttaatgcaa ataagtgatg
     3901 catttgattt agtaaaatta tcacaagaat ttattgatga agtaaagcca aaaatcgaaa
     3961 ataactaccc tgaaattgca ccatttgtag gtaaattaaa gggtgatgat atagaagaag
     4021 gtaaagaatt agtagcaaca cacatagctg aaggattata ccatgatgga aaatttgttg
     4081 gttatgtaaa aagagcacat gatgtagatg ttaacttaaa tgcccataca atgtttgaaa
     4141 atctagtagt aaaagcatct ggagttttat ctgctattca aatgctaaga catagcaaaa
     4201 tagaccctgc tgaaatagat tatgttattg agtgttcaga agaagcttgt ggagatataa
     4261 accaaagagg tggaggaaac tttgctaaat caatagctga gatagcagga cttcaaaatg
     4321 caactggttc tgacacaaga ggattttgtg cagcaccaac tcatgcatta attcaagcag
     4381 cagctttagt aaaagctggt atacataaaa atgtaatggt agtagcaggt ggagcaagtg
     4441 ctaaacttgg aatgaatgct aaagaccatg ttaaaaaagg tcttcctgta cttgaagatg
     4501 ttgttggagg atttgcagta cttgtatctg aaaatgatgg tgtaaatcca gttataagaa
     4561 ctgatttaac aggtaagcat actgttggaa ctggttcttc accacaagca gttatgacag
     4621 cacttataac ttcaggtctt gatagagcta atttaaaaat aacagatgtt gatgtgtatt
     4681 cagttgaaat gcaaaaccca gatataacta agccagcagg agcaggagat gttccagaag
     4741 caaactacaa aatgataggg gcacttgctg ttaaacgtgg agacttagaa aagaaagaat
     4801 tgaaagattt tgtttctaac aaaggattac caggatgggc accaactcaa ggacatatac
     4861 catcaggagc tccttatata ggatttttaa tagatgattt aactacagga aatagaaata
     4921 gagctatgat agttggaaaa ggtagtttat tcttaggaag aatgactaac ttatttgatg
     4981 gagtatcttt tatagctgaa agaaatacag gtgttactga agagacttca ggaatatcta
     5041 aggatgaaat taagaagata atagctgaat ctatgaaaaa actagcatta gatatgttag
     5101 aagaataggg gtgaggatat gtcaaaaaaa gtaatcgcag atgtattctt agaagtagct
     5161 aatgcaatag aaagcggcga atttggcaaa aaagtaaaaa ttggtgttac tacacttgga
     5221 agtgaacatg gtgtagaaaa catggttaat ggtgcacaat tagcaaaatc taatttattt
     5281 gatattgtgc ttattggacc aaaagtggaa actgaccttg aagtagttga agttaatgat
     5341 gaaaaagaaa tgcatgcaaa aatggaagaa ttattagatt caggatatat tgatgcttgt
     5401 gttactatgc attataattt tccaataggt gtatctacag taggtagagt aataactcca
     5461 gcaaaaggaa aagaaatgat acttgcaaca acaacaggaa caagtgctac taatagaata
     5521 gaggcaatgg taagaaatgc catttatggt attgctacag ctaaatcaat gggtaacaaa
     5581 tgcccaaaag ttggaatact taatgttgat ggagcaagac aagttgagaa gtgcttaaaa
     5641 gaattaaaag ataatggata tgacatggag tttgcggatt caattagagc agatggtgga
     5701 tgtgtaatga gaggtaatga tttacttgta ggtgcacctg atgttatggt tactgataca
     5761 ttatctggaa atatatttat gaaagtattt tcttcatata ctacaggtgg agattatgaa
     5821 gctcaaggct ttggatacgg acctggagtt ggagaggact atgatagaaa ggtacttata
     5881 gtatcaagag catcaggttc tccggtagta gcaaatgctt taaaatacgc ttatgatgtt
     5941 gtaaagggag atataagtaa tgttgcaaga aatgaatttg caaaggttaa aaaagcaaaa
     6001 tttgatgaca ttatatcctc tcttactaaa aaagaagtta aagctgaaaa agtagaagtt
     6061 aaaatgccag ataaagaaat tgttactcgt caaatagctg gtgttgatat aatggattta
     6121 gaagatgcag tatctgagtt atggaaaaac ggaatctatg ctgaaagtgg tatgggttgt
     6181 acaggtccaa tagttttagt aaatgaagct aaaggagact tagcagttga aactttagtt
     6241 aaagctggat atactgcaaa ataagtacat tttttatatg taaataagaa catatagtta
     6301 taattttatt gattataatt atatgttctt tttattttat atcttgtcaa tatttatata
     6361 ttaacacaat ccatttattt gattctctag ctaatttatt atatttattc agttaaaatt
     6421 aagctttcta actaaataaa aactaatgaa gtattaaaat gaaattgata aaatatgaaa
     6481 aaattttagg aataaagtat ctgttagata aaaataagat taattagtga tataatataa
     6541 ttaagataaa atttattatt tggaggcaaa atcatgagca gagtaaaaaa tgtagtagaa
     6601 ttgttggaaa ctaaaggtgt tgatgcacta tatctaacaa agaaaactaa tgtaaactat
     6661 ataagtggtt ttcctgatga agaagcatat gcagtgattt gcaaagatgg aaacttttta
     6721 gttactgata gtagatatat ggagcttgca gaaaaggttt gcaaagattt tgaaattata
     6781 aattggcata attttgatag aagtgtggca aaggcagtta aaagtgtatg tgataaagtg
     6841 ggtataaaga aacttgggtt tgaaagaact aatatagtgt ttgataaata tgaagaactt
     6901 aaaaatctga ttgagaagga taatggagaa ttaataccta cagaaaatat tgtagagact
     6961 ttgagatatg taaaagacaa agatgaaatc aaaaatacaa gaaaagcttg tgaaatagcg
     7021 gataaggcat tagaagaatt gattccacat ataaaagctg gagttagcga aattgaactg
     7081 gctacaaagt tagagtactt tatgaaaatg aacggagcac aaaatatagg gtttgaaact
     7141 atactgatat caggagcaaa gacttcattg cttcatggta agcctagtga taagattata
     7201 gaaaaaggtg attttgtact tatagactat ggcgctatgt ataatggcta tatatctgat
     7261 acaactagaa cttttatagt tggaggagct tctgaaaagc aattagaaat atataattta
     7321 gttaaggaag ctcaaaatgt aggggttgag aacatgaaag ctggtgtaca tgcgacaatt
     7381 ccagatgctg agataagaaa agttgtcaaa aaatatgaag actattacta tcaaggtata
     7441 ggacatggag tcggtagaga tgttcatgaa gaacctttca ttggtaatta tggtgataaa
     7501 atcatagaag agggatgtat aataacaatg gaaccaggta tttatttccc tggatggggt
     7561 ggagttagaa tagaagatac tgttttgatt actaaaaatg gtccagaaag acttactaaa
     7621 ttccctaaag atttgatgat tttagacaaa taatttaatt tatatatatt tttttacata
     7681 aaatataaca taatctcact attaagctct atttacatat tatagtaaag agggcttaat
     7741 tttattgtga gaggtgatag tatatatgta taaaaaacat aaatttataa aaaatataaa
     7801 aaaagcctgg tataaaatca aattaattga tagatgcctt ataattgtaa tggcaatatt
     7861 aatgtttcaa tctatatata atttatttgt taatgaagta aattcacaag atactacaac
     7921 tattgatgta gtagtaagaa ccacttcagc agctatattt gggtattttt taagtgctaa
     7981 ttttataaag agacctttaa ggaagtatac aaatactgat gtatcttcaa atctttttat
     8041 agataataat ttatccccaa aaggtcaaga gtctaatcaa aataatataa tgaatgtcaa
     8101 aaataccatt ggatttactt cagaagctga taattatgaa aagaaaatac ctattaataa
     8161 taatgaaggt tttgaagaag gagaaactag tgaattgcaa attattatag ctactgtaat
     8221 ttgtatagta gcattaacag tactttttgt tgtaagaaat tttacaacta caacatcagc
     8281 ttctttaggt actatttcac agatgagaga ttttgtctca ggatgcgtgg gttttttgct
     8341 aggttgtcca agtaaacctg attaaaaaag tattatcaat aaaaggtatt ttaaaaagta
     8401 tcagtataat aatatttaga tttaaatgta tacaattata attctaaaca ttctatattg
     8461 tatttttata ctgatactct aaactatact atttattaaa atattttaac attgtattta
     8521 aacaaaatat tatatctgta taatataaat ttagtttata tgaatagatg ccatatactc
     8581 aataaaaagt ttcatactat gagatatgta ttgatttctt ctccaaagaa ccccaatatt
     8641 cgcagtaatt gcacgttcta atggtattga acagagattt tctgtatttt gaaatatttc
     8701 tttatacaat attgttgaag ccaagtcatt ttctagtaaa taacgtatgg tagaaagttg
     8761 atttgagtgt aaaattatat ttggcttagc tttttcaagt ctttttgtaa taatcttata
     8821 gtgataagaa ccttcagata agattacaaa aggtacattt tttaacatat aattcgatat
     8881 aatgggatgt tttgccaatt catttttggg atttgtaact aagtataatt ctgttttata
     8941 gattgtcttt gaatctatat catcataaat atcactgtca attataccaa tacaaaaatc
     9001 aagtgtagat tcttcaatca ttttagctcc aacaaatgtt ggaacttcaa aaattttaag
     9061 ctctatatct ggataaatta cattaaatct aggaagtatt tttgaaagca aaaacgtacc
     9121 taaaactgta ggaataccta ttttgagaga agcttttctc ttttttccta aatcaagtgc
     9181 gtcacattga aaatcctcaa attgttttat aaaatgtttt gacttgtcta aaaaaatttt
     9241 tccttcaggc gttaactcta ttttattacc tattctgtaa aaaagttcaa agccaagttc
     9301 tttttctaat tctttaattg ctaatgttat ggatggttga gaaatatgaa tactttccgc
     9361 agccttagtc atgtttttat atttacagac agtttgaaaa tagtataaat agtttatatt
     9421 catttggtat tagttccttt catttacata ttagtgacaa gtatataatt aacaatagca
     9481 taagttttgt atattgttgt aaaaaaatag aatagtttta aactatcata gcataagttt
     9541 ttgatatttt caatctatta agaatagtgc tattattaaa ttaacaaaag agaatagctg
     9601 gtatggttta gagtaaatca atctatatta cagttatatc aacgaatatc aaaaaattat
     9661 ctattagaaa ataaaaatta tttttacaat taaattgtta attaaatcat aaagaaaaaa
     9721 gtgatattaa caagataaaa tttttttaat cagaaatgtt aatttaataa caaaatagag
     9781 tattggggag aaaggagaaa tatatttttt aataatattt gttaatttaa taacgaaaaa
     9841 tgaaagggag tgatttagtg aagtttgaat tgatggattt tttaatgaat ccttttgtcc
     9901 ttatgtttgc tgcagtaatt acaggaattt tatttggaaa aattaaattt gggaagttta
     9961 attttggggt atcaggagca ttatttacag gattatttat tggatggctt gcatatagct
    10021 tggggaatct aataatagca aaaggggaaa cagcagcagg atataaggca gcaactgtaa
    10081 tgatgggtaa tggaattatt tcttctgact tctttgattt ctttttaata atatttgttg
    10141 cagcagttgg tctattggca gcaaaagata tgaaagctgt attaaagaag tatggagcaa
    10201 gatttgtaat tttaggagtt cttataactt ttataggtgg ctctatgact tatgcaatga
    10261 ctttactatc aagtgataaa ggaagtagtg cttatgaagt ttctggcgtt tacacaggag
    10321 cattaacaag ttcgcctgga ttagctgctg ctttagaaac agctggtaaa catgcagaag
    10381 atgtatcaaa agaatttgaa aaagcttcaa taaaagataa aaaagaaatt ttaaaagtag
    10441 tcgacccaga aggcaaattg gatgtgaata caactacatc actgactcaa gagcaaatag
    10501 ataagtatat tgcatatgct gaagctggtg ttggtatagg tcatgcagtc gcatatcctt
    10561 ttggagtttt aatagtaatc ttaggtgtaa atttccttcc aaaattattt aggatggacc
    10621 taaaagaaga aagaagaaaa tatgagaagg aaatgaagga agcaagagat agtgtttctg
    10681 gtaaaacgat accagaagtt ccatttaata taataacatt tttcttgaca tgtttagctg
    10741 gatacttagt tggaggaata catgtattta tgggaccact agggtacttc acactaggcg
    10801 caacaggagg ttcactaatt gtatctctag ttcttggata tataggtaag ataggggtag
    10861 taaatttcag aatggaagaa aaggttttaa atattttaaa acaaataggg ctagtatttt
    10921 tcttagcaat agttggatta agatatggtg gaaaagtagt tgattcaatt atgacatctg
    10981 gtatgcacct agccttagta gcaatagcag taggtgttac agctatgatg ataggattct
    11041 tagttggcaa atacgtattt aaattaaatt ggattttatt atctggagct gtctgtggtg
    11101 gtatgacatc aacaccaggt cttggagctg ctgttgatgc tcttgatagt gatgacccag
    11161 cagcaggtta tggtgctacg tatccatttg cattattaac taaagttata ttggttatag
    11221 ttttacacaa acttccaatg taaataagat tttatatggg gggatattta tgaataatga
    11281 aggagcattt gaagaattaa gtggaagatt aagaaataaa gaattgtgtg atttaataat
    11341 gaatgcagat gaagctgcta aaattataga ggatggcatg gttattggag ttagtggatt
    11401 tacaccttca ggctatccaa aagcagtacc attagcagtt tcagaaagag ctaaaagtgg
    11461 agaaaagata aaattaaccg tatattcagg agcatcttta ggtccagaag ttgatggagc
    11521 atggtcagaa gcaggtataa ttgaaagaag acttccttat caaactaatt caatacttag
    11581 aaataatatt aataaaggtg ttgttgatta tatagatatg cacctaagtc attctactca
    11641 atttttaaat tatggaacta tacctaaagt agatgtagct atagtagaag cacttgcaat
    11701 aactgaagaa ggaaatatta taccaactag tgggataggt aattctccat cttttataaa
    11761 aagtgcagat aaagtaatag tagagataaa tcttgcaaaa ccaatggaaa tggaaggtat
    11821 ggcagatata tatattacgg aaaatccacc aaatagaaaa ccaatagaaa taaatcatcc
    11881 taaggataga attggaacaa catatatacc ttgtggtttg gataaaattg caggagtagt
    11941 aattaccaat atgcaagata agacccgtcc attgggagtt gtagatgaag cttcgaaaaa
    12001 aatatcaaat aatattattg cttttttaag agaagaagta aaggcaggca gattatcaaa
    12061 gaatttactt cctcttcaat ctggtgtagg aagtgtagca aatgcagtat tatatggatt
    12121 atgtgaatct gaatttgaaa atttaacttg ttatacagag gttgtacagg actctatgtt
    12181 ggatttaata agaatgggta aagttacaat ggcatctact acatctgtaa gtccatctcc
    12241 agaaggatta ataaaatttg aaaaagatat tgattttttt aaggataaga ttatattaag
    12301 acctcaagaa attagtaaca atccagaaat agccagaaga attggtgtaa ttgcaatgaa
    12361 cactgcaatt gaagttgata tttatggaaa tgtaaattct actcacataa tggggtcaaa
    12421 gatgatgaat ggaattggtg gttctggtga ttttgctaga aatggagcaa taactatatt
    12481 tagtactgag tcaattgcaa aaaatggtga tatatcatca atagtaccta tggtatctca
    12541 cgtagaccac actgaacatg atgtaatggt aattgtaaca gaacaaggat atgctgattt
    12601 aagaggtctt gcacctagag aaagagctat aaaaataata gaaaattgtg cccatccaga
    12661 ttataaagaa caacttaggg attatttaaa tagagcttgt aaaagtggag caaaacaaac
    12721 acctcacata ttggacgaag ctttatcatg gcatagtaaa tttatgagta caggtacaat
    12781 gaaaaaggct gaaactttta aaagtgcatt ataaaatatt taataaattg gaggtagatt
    12841 ttaaatggaa aaagcagtgg aaaactttga agatttaagt aaggaataca ttaatggtta
    12901 tattgagaga gcaagaaaag ctcaaagaga gtttgaatgt tatactcaag aacaagtaga
    12961 taaaatagta aaaatagttg gaaaagtagt gtattataat gctgaatatt tagctaaact
    13021 tgctgtagaa gagactggaa tgggagtata tgaagataag gtagctaaaa ataaaagcaa
    13081 agcaaaagtt atatataaca atttaaaaga taaaaaatca gttggtataa ttgatataga
    13141 tagagaaact ggaataacaa aggtagctaa accagttgga gtagtagcgg caataactcc
    13201 ttgtacaaat ccaatcgtta cacctatgag caatgcaatg tttgcactta agggaagaaa
    13261 tgcaattatt atcacacctc atcataaagc aattggatgt agtacaaaaa ctgtagagat
    13321 gattaatgaa gaattagaaa aaataggagc tccagaaaat ttaatacaaa tacttgacca
    13381 acaatcaaga gaaaatacaa gaaatttaat ttcatcagca gatgtagtaa ttgccactgg
    13441 aggaatggga atggtaaaag ctgcgtactc aagtggaaaa ccagcacttg gtgtaggggc
    13501 aggaaatgtt caatgtatca tagatagaga tgtagacatt aaagaagcag tacctaaaat
    13561 catagcagga agaatttttg ataatggtat catttgttca ggtgaacagt cagtaatagt
    13621 agcagaagaa atgtttgata aaataatgga tgaatttaaa aataataaag gttttatagt
    13681 tagagataaa gtacaaaaag aagctttcag aaatgctatg tttgtaaata aatctatgaa
    13741 taaagatgca gtaggtcaat cagttcatac aattgcaaaa attgcaggtg ttgagatacc
    13801 agaagataca aaaataatag taatagaagc tgatggacca ggagaagagg atattattgc
    13861 aaaagaaaaa atgtgtccag ttatatctgc atacaagtat aaaagttttg aagaaggtgt
    13921 agctattgca aaagcaaatt taaatgtaga aggaaaagga catagtgtat ctatacattc
    13981 aaatacagtt aaaaatatag aatatgcagg agaaaatata gaagtatcaa gatttgttat
    14041 taatcaatgt tgtgctacta gtgctggagg aagtttcttt aatggtcttg cacctactaa
    14101 tactctaggt tgtggttctt ggggaaataa cagcatctct gaaaatcttg attataagca
    14161 tctaattaat atttctagaa ttgcttatta tatgccagaa aatgaagtcc ctacagatga
    14221 agaactttgg ggataaaatt tagacaaaaa atattcattc taaattaaag taggaatata
    14281 ttttaattta gaatgaattt aagacataaa ttaaatttaa attagtttat tggactaaat
    14341 ttaaaatatt aagagacata tagaagaagg aagtattata atttaaaata aataaaaaaa
    14401 tctataaaag agaaggagtg gttttattat ggcattaatg acaggagctc aatatattga
    14461 aagtttgaga aaattaaata ctaaggttta tatgtttggt gaagaggtta agaattgggt
    14521 tgaccatcct atgattagac catctataaa ttgtgtagca gcaacttatg acttagctca
    14581 tgacccagaa tatgcagatt taatgacagt tacttcaaat atcacaggtg aaaaaataaa
    14641 tagatttggt catcttcatc aaagtgttga tgatttgata aaaaaagtaa aaatgcaaag
    14701 actatgtggt caaaaaacag cttcttgttt ccaaagatgt gttggtatgg atgcgtttaa
    14761 tgcagtgtat tcaacaacat ttgagtgtga taaagcccat ggaactaatt atcatgataa
    14821 ttttgtaaaa tacttaactt atatacaaga aaacgattta gtagttgatg gagctatgac
    14881 tgaccctaag ggagatagaa gtctatcacc tagtgctcaa cctgacccag atatgttcct
    14941 tcatatagta gaaagaagag aagatgggat aattgtaaga ggagcaaagg cacatcaaac
    15001 tggttcaata aattcacatg agcaccttat aatgcctact ataagtatga ctgaagcaga
    15061 taaagactac gctgtatcat ttgcagttcc ttcagatgct gaaggagtgt ttatgattta
    15121 tggaagacaa tcttgtgaca ctagaaaatt agaagaaggt gcagatgttg atttaggaaa
    15181 taaggagttt ggaggtcaag aagctttagt agtatttgat aatgtatttg ttccaaatga
    15241 tagaattttc ttatgtggag aatgggattt ctctggtatg ttagtagaga gatttgcagg
    15301 ataccataga caaagttatg gtggatgtaa agtaggagta ggtgatgtaa taataggagc
    15361 agcagcttta gcagctgatt ataatggagc aaacaaagct tcacatataa aagataaact
    15421 aatagaaatg actcacctta atgaaagctt atattgctgt ggtatagcat gttcatcaga
    15481 aggtcacaaa acagaagctg gaaactatca aatagattta cttcttgcaa atgtgtgtaa
    15541 acaaaatgta actagatttc cttatgaaat agtaagactt gcagaagata tagctggagg
    15601 gcttatggtt actatgcctt cagaaaaaga ctttaaatca gaccttaaag ttggaacaag
    15661 tggaatgact attggcgaag tatgtaacaa gtattttaaa gcttcttcag tggcttcaac
    15721 agaagaaaga atgagaatat taagattctt agaaaatata tgccttggtt catctgctgt
    15781 tggttataga actgagtcaa tgcatggggc aggttcacca caagctcaaa gaataatgat
    15841 ttcaagacaa ggaaacataa atcaaaagaa agaattagct aagaaaattg cgggaataaa
    15901 aaaagaggaa gctttaaatt tataaaaagt tgatatataa agggaaatta tatattaatg
    15961 tgtgttaatt aatgaaaaaa tatgtagtat aagagatagt tataaaatta tggtggtttt
    16021 tagctaagta aagaaatttt taataaggag aatttacaca tgacctgtgg agttagattt
    16081 tgtgggggct gtaaccctag atacgaaaga gggaaggctc ttacaataat caaagagcac
    16141 ttcaatggta gggtagattt tagtatagca caagagaacg tcgagtatga tttgttactt
    16201 ataattggag gatgcgcaac tctatgcgca tcctatcaac aatataaagt gaaaaatgaa
    16261 tgtgttaaga tgtgggattt tagccatata gaaagagtta taaatgtgat tgaatcagca
    16321 atttgtgaaa cttaaaaaag ctaaatgggg ggatttcatt gagttggcaa gaattgtatc
    16381 aaagtaaatt atgtagtgct acagaagctg taaaacagat taaaaacggt gatactgtag
    16441 tatttgcaca ttgtgtaggt gaacctcctg cactagtaga agctatgatt gaaaatgctg
    16501 aacaatataa agatgttgag attaaacata tggtaagctt gggtagtggt ggatatactg
    16561 ctaagggaat ggaagctcat tttagagtaa atccaatgtt tgtaagtgga aatgtaagaa
    16621 aggcaattga aaatggagat ggtgatttta cacctgcatt cttccatgaa gtaccaaagt
    16681 tgcttagaga aaaaagatta aaatgtgatg ttgttttagc acaggtaact ccaccagatg
    16741 aacatgggta ttgtagctta ggaacatcag ttgattatac ttatgaagcc attaaaactg
    16801 ctagaacagt aattgttcaa gtgaatgacc agtttcctag aacctatggt gaggtagtcc
    16861 atgtatcaga gtttgactat atagtggaaa aatcacaacc tttatttgaa ctacaacctg
    16921 caaagattgg agaagtagaa gaagcaattg gtaaaaattg tgcttcatta attgaagatg
    16981 gctctacttt acagctaggg attggtggga taccagatgc agttatgtta tttttaactg
    17041 ataaaaagga tttagggatt cattcagaaa tgatttcaga tgggactttg gccctttatg
    17101 aaaaaggtgt tataaatggt aaatataaaa attttgataa agaaaaaatg acagttacct
    17161 tcttaatggg tactaaaaaa ttatatgact ttgcaaataa taaccctgca gtagaggtaa
    17221 aaccagtaga ctatgtgaat catccagcaa ttataatgaa acaacataag atggtttcta
    17281 taaattctgc tatacaagtt gatttaatgg gacaggtagt tgcagaggct atgggactta
    17341 gacaatttag tggtgttgga ggtcaagttg actttatacg tggagtgtct atgggtgaag
    17401 atggaaaggc aattatagct atgccttcaa taactacaaa aaaagatggt acagtaattt
    17461 caaaaatcgt atctattgtg gatgaaggtg cacctataac gacatcaaga aatgatgttg
    17521 actatattgt aacagaatac ggtatagcag aattaaaagg aaaatctctt agagaaagag
    17581 caagaaattt aataaatata gctcatccaa gtgtaagaga acatctagca gtagaatttg
    17641 aaaagagatt taaggagaaa tattaataat agaggggtgt taataatgga agcacttaga
    17701 gtagttccaa agatatttta ctttgataca tttaaagaat ttaatgaaga atttaaaata
    17761 ggaaaaaatg atttagtaat tactaatgag tttatatatg agccatatat gaaacctctt
    17821 ggaatagata caaatttaat ttttcaagaa aagtttggaa caggtgaacc atctgatgaa
    17881 atgatagact ccatgacaaa ggaaatgaag aaatacaatt ttgatagaat tattgcattt
    17941 ggaggaggaa caatagttga tatatgtaag atattagcac ttgatgttcc agaaaagtct
    18001 attgatttat ttgaaggaga agtatcacct aaaaaagtaa aagagttagt agtagttcct
    18061 acaacatgtg gtacaggtag tgaagtaaca aatgtagcta ttgcagagtt aaaatctaaa
    18121 catactaaaa aggggcttgc agtagaagaa acatatgcag actatgcagt gttaattcca
    18181 gagacagtaa aagggttacc ttacaaattc tttgttacaa gttcggtaga tgctcttata
    18241 catgctattg agtcttatct ttctccaaaa gcaagtccat ttacagagat gtattctcta
    18301 caagcaataa aaatgattat ggatgggtat aagaagatag ttgataaagg agaagaagag
    18361 agatttaatc atcttagaga ctttgtactt gcatcaaatt atgcaggaat tgcttttgga
    18421 aatgctggtt gtgcagcagt tcatgcacta tcttattcaa taggtggagc tttccatgtt
    18481 gcacatggtg aagcaaatta tcagttcttt actgaagtat ttaagatgta ttctagaaaa
    18541 aaacctaatg gaaaaattat aaaatgtaca aaaattttag cagatgccct tgaatgtgac
    18601 cctaactgtg atgtttatgg agaattagaa aagtttttaa ataaattaat agctaaaaaa
    18661 gctttgagag aatatggaat ggtggaaagt cagatagatg aatttacaga tagtacaata
    18721 gcaaatcaac aacgactact tgctaataat tatgtagagc ttagtagaga ggaaataaga
    18781 gagatatttg caaatcttta ttaatacaat tttttattta tatgtatagt caacataaac
    18841 aatgctaatt atatttatgt acaaataaaa ataagggaac ttaaatttta attaaagtta
    18901 agttctctta tttttatatg gagttaaata taagtatatt tttaatttaa aatatgctta
    18961 tatttaagtc tattacttta tttgaaacaa tttgtagaat ttcaaattcc ttgacagtaa
    19021 taagtaagta attgtataat caaactgcaa tatattaata atattagtga aaatgaaagg
    19081 attataaaat atgtataaat cagaaaattt ttcaaaaaaa tctgtattaa taaattcatt
    19141 tttaagtaaa tttttaaaaa taattggatg gggtttggta agcttattta cattatcagc
    19201 cttagtactt tcaactattt ccattataat tggatgggct gtaatacttc ctattcaagt
    19261 atgtgcagta tttttactta taggaggagt ttgtattttt aaaggtaaga gattaggaga
    19321 ccaaatttct cgttataaaa aatattgtac aattataaac aatagaaata ttattccagt
    19381 agaacttatt gcagaaaaca catctaaatc attaaatttt ataataaaag atgtacaaaa
    19441 gatgatagac aaaaactact ttataaatac ttatattgat aaaagaaata atcagattgt
    19501 gttaacaaat gaagattttg ttccaccaag atatgaagat gtagcatatg aagtcaaaga
    19561 agaagaaaag actgatgaat ccaatgaagt tgatacaatt ataaataaag gaatgaatta
    19621 tctaacacaa ataaaagaag ctaataaaaa tattaaatca gaatcaatgg gcgataaaat
    19681 tgtacaagta gaagatgtta caagtaaaat atttgatgtt gtaaaacatg acccatcaaa
    19741 acttactcag atacaaaaat ttatggatta ttatcttcca accactttaa aactattaaa
    19801 ttcatatcat acattggaag aacaaggaat tgatagagaa aatataacaa caactatgga
    19861 aagtattgaa aatacaatga atacgattgt agtagctttt gaaaatcaat tggactatct
    19921 gtttgaagat gaagccattg atatttctac agatattact gttttagaga atatgttagt
    19981 ccaagaagga ttaacgagtg gtaaatttta acttcaatag aaaaaatatt ctgttatttt
    20041 taaacaatta attagtcata aagtataaaa tgtgtattat ataagttaat cagtacttta
    20101 tgaaggaaga ggagagaaag atacaatgaa taatttagat gatataccag ttatgccaac
    20161 tttgacactt gacccatttg gagaagaatc aaacactaat gatatagata attctgactt
    20221 attaatgaaa aaggatgaaa aagacccaga agaagaaaaa ctgtctgaat ctgaacgcaa
    20281 gatggtaaag gaatttgcgg aaaaaataga tattacaaat acaaatatga ttttgcaata
    20341 tggagctgga gctcaaaaaa aggtagcaag tttttctgaa acagctttaa agagtgtaaa
    20401 aacaagagat ttaggagaag ttggagatat gcttacaaat ctagttactg atttaaaagc
    20461 attttcagca gatgaaaaag aacaaagtgg ttttttagga atttttaaaa aggcaaataa
    20521 taaaatttca aatttaaaaa ctaagtatga tagtgcagag gtaaatgtgg ataaagtaag
    20581 caaagagctt caaaagcatc aggttaaaat attgaaagat atagcaatgc ttgataagat
    20641 gtatgaattg aacttagctt actcaaaaga gcttactatg tatattatag caggaaaaca
    20701 aaaattaaag gatatgaaag aatatgaaat gccaaagttg agagaaaaag ctcgtttatc
    20761 tggttcaaca gaagatgctc aatctgtaaa tgatatggtt tcactttgtg acagatttga
    20821 aaagaagatt catgacttag aattaactag aatggtatcg cttcaaatgg ctcctcagat
    20881 aagattggtt caaaatagta ataatctgat ggcagaaaag attcaatcaa ctatagtaaa
    20941 taccattcca ttatggaaaa atcaaatagt gcttgctctt ggaatttctc acttaaatca
    21001 agctatgaaa gcacagcgtg aagttagtaa tatgacaaat gagcttttat taaagaatgc
    21061 agaaactttg aagatgggaa caatagaaac agcaaaagaa tcagaaagag gaattgtaga
    21121 tatagacaca attaagaaaa ctaatcaatc tctaatatct actattgatg aggtagttaa
    21181 aatacaacat gatggtagaa taaagagaca agaagcagaa gttgaattat caaaaataga
    21241 aaatgaactt aaaagtaagc ttcttgaatt tacagttaaa taaaaattat actaatataa
    21301 aaagagactg tctcaaaata gagataagtc tctttttcat tgtttaaatg attaaagtat
    21361 atttatatta taaatttcta ttatctttta acataacatc atgagctcca ccttcaacta
    21421 tactagtaga agatacaagt gtaagtttag catttttttg aagttcagga atgctaagtg
    21481 ctccacagtt acacatagta gaacgaactt tacttaaaga aagagttaca ttgtctttca
    21541 aagaaccagc ataaggcaca taagagtcaa caccttcttc aaaagaaagt ttcttatctc
    21601 caccaaggtc atatctttgc cagtttctag cacgagcaga accttctccc caatactctt
    21661 tcatataact accatttata ttaactttgt tagttggact ctcatcaaaa cgagagaagt
    21721 atctaccaag cattataaaa tctgcaccca tagctaaagc taaagttata tgatggtcat
    21781 aaacgatacc accatcagag caaattggaa tataaatacc tgttttttca aagtattcgt
    21841 ctctagcttg agcaacttca attatagaag tagcttgtcc acgtcctata cctttttgtt
    21901 cacgagttat acatatagaa ccaccaccaa taccaacttt aacgaagtca gcaccagctt
    21961 cagctaggta aaggaatcct tcgcggtcaa ctacatttcc agctcctacc ttaactgtat
    22021 caccatattt ttcacgaatg aaatcaagtg ttattttttg ccactcagaa aaaccttctg
    22081 aagaatctat acataaaaca tcagcaccag cttcaactaa agcaggaaca cgttcagcaa
    22141 agtctctagt attaatacca gctccaacta catatctctt agaagaatct aaaagttcaa
    22201 gtggattttc tttatgagaa gagtaatctt tacggaatac catatacata agattaccat
    22261 tatcatcaag tacaggaaga gaattaagtt tgtggtccca tataatattg tttgcttcct
    22321 ttaaagtaac atctttgtta gcataaacta tagatgacat aggagtcata aattcactaa
    22381 ctttagtatc taaatccata cgacttatac gataatcacg acttgcaaca atacccaata
    22441 atttaccagt tgaagttcca tcttcagtaa cagctactgt agaatgtcca gttttttctt
    22501 ttaagtcaag tatgtctttt aatgtgttat caggtttaat atttgaatca cttacaacaa
    22561 aaccagcttt gtgggattta acacgtgcta ccatagcagc ttcattttca atagtttgag
    22621 aaccgtatat aaatgaaata ccaccttctt tagcaagtgc aatagccatt ttgtcatcag
    22681 aaacagattg cataatagca gaagtcaaag gtatattcat ataaatatct gcttcctcgc
    22741 cctttttaaa tttagtaaca ggtgttttta aacttacatt agcaggtata caatcagtag
    22801 aagagaaacc tggtacaaga aggtattcac tgaaagtatg tgatggtgta tcaaaataaa
    22861 acgccatttt ttaatcactc cttatatttt tatacttttt ataattgata gtattaatta
    22921 taacttattc tttaatttta ttaaagtatt ttgtcaaata ttgagatttt tgaaaaattt
    22981 aaactatttt aattaaattt gtttaatata tattgcctta tagggtaaag ttgtatatta
    23041 aataatgttt agtgtttaat agtagctaat ttaaaattaa tctttagtga ttcatataat
    23101 tacatttgta tagttt
//
LOCUS       DAMJZE010000044        22231 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJZE010000044 DAMJZE010000000
VERSION     DAMJZE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.044"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            155..325
                     /locus_tag="PC541_001563"
     CDS             155..325
                     /locus_tag="PC541_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2839031.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            complement(501..1040)
                     /locus_tag="PC541_001564"
     CDS             complement(501..1040)
                     /locus_tag="PC541_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2839032.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(1179..1811)
                     /locus_tag="PC541_001565"
     CDS             complement(1179..1811)
                     /locus_tag="PC541_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2839033.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            1947..3860
                     /locus_tag="PC541_001566"
     CDS             1947..3860
                     /locus_tag="PC541_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2839034.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            3876..5252
                     /locus_tag="PC541_001567"
     CDS             3876..5252
                     /locus_tag="PC541_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2839035.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(5392..6042)
                     /locus_tag="PC541_001568"
     CDS             complement(5392..6042)
                     /locus_tag="PC541_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2839036.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            6513..8525
                     /locus_tag="PC541_001569"
     CDS             6513..8525
                     /locus_tag="PC541_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839037.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            8894..10114
                     /locus_tag="PC541_001570"
     CDS             8894..10114
                     /locus_tag="PC541_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839038.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            10151..11500
                     /locus_tag="PC541_001571"
     CDS             10151..11500
                     /locus_tag="PC541_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2839039.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            11614..12176
                     /locus_tag="PC541_001572"
                     /pseudo
     CDS             11614..12176
                     /locus_tag="PC541_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12284..14617)
                     /locus_tag="PC541_001573"
     CDS             complement(12284..14617)
                     /locus_tag="PC541_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2839040.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(14614..15297)
                     /locus_tag="PC541_001574"
     CDS             complement(14614..15297)
                     /locus_tag="PC541_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839041.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(15368..16609)
                     /locus_tag="PC541_001575"
     CDS             complement(15368..16609)
                     /locus_tag="PC541_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2839042.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(16620..17306)
                     /locus_tag="PC541_001576"
     CDS             complement(16620..17306)
                     /locus_tag="PC541_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839043.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            complement(17544..17888)
                     /locus_tag="PC541_001577"
     CDS             complement(17544..17888)
                     /locus_tag="PC541_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839044.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            complement(18219..20726)
                     /locus_tag="PC541_001578"
     CDS             complement(18219..20726)
                     /locus_tag="PC541_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839045.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            complement(20735..21397)
                     /locus_tag="PC541_001579"
     CDS             complement(20735..21397)
                     /locus_tag="PC541_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839046.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
BASE COUNT         7893 a         3140 c         2742 g         8456 t
ORIGIN      
        1 ataaaaatat aagcagatta aactttattg taaaacttta aaacaagaat atcataataa
       61 agtttttgtt gtagaattaa tttaattgtt atattcaata atatttgtta taatgttatt
      121 gaattacaat acatataaaa gggaggattt caaaatggca tataaaatta ctgacgcttg
      181 tataagctgt ggtgcttgtg aggctgaatg ccctgttagc tgtatatcag ctggagacga
      241 cgcatatgtt atagatgctg gttcttgtat agactgtggt tcttgtgcag gagcttgtcc
      301 tgtagatgca cctcaaccag aataataatt cgttattcat aaagagactg attttatttt
      361 ataaatacta atcagcctct ttttaattat gtatgattca attaatatta tttatatact
      421 ttattatgtt gctacctaat tcatcttaaa atactgttag cacatatcta atacttttaa
      481 ctttaatcta atttataatt ttaatactcc tcataaaatc tttcatattt attaaagcaa
      541 tcaagacata ctttctttcc ttcttcaatc cttatatatt gctcagatgc aaattcacca
      601 cacacttcac atttaagaga tttaaatatt cttgcttttt caggaagtct taacttagta
      661 tctgtaatct caaatatttc ttcaaaatca ctttctaata agtacttttt gtatccttct
      721 ctatccattt gaaatggctt tgactttaca tatattctta tagattttcc attttcccta
      781 ttaaagaaat gatatactga tttcccagtc atcctaagta gtaaatttcc tttacccatt
      841 gtgcaactta gcacgacttg tataccatct attccacaag catcattttc agaaatacat
      901 acaatttcct catctcctga ctgtttacat cctaaatatt ctcttgctgc aagtgctgct
      961 ttaaatccta tagctaaccc tggacattca tgaccatgaa attcaattgc ctttttccat
     1021 aattcctcca caaaaaccac tctccttaat attgtttatt tccatacatt atttaaattt
     1081 ttatcctaat atttcaaaaa gccagaggat ttaatattaa atcttctgac tttctcaata
     1141 ctcttcacaa ctgctcatta ttaatatgtt taagttgtct actttccttc tttcattaaa
     1201 tacgataagg taaataaact ttctgttaag tttacatttt caactataac acctttcgga
     1261 acttctaaat ctaaaggtgc aaaattccat acacctttga ttccagcttt tacaactcta
     1321 ttaataactt cttgtgctcc atttttaggt atacataata cagcaatatc tatattattg
     1381 ttttttataa agtcttctaa agtatctgaa tctaatactt caaactctct tatctttaaa
     1441 cctatcattc taggatttgc atcaaataaa gcttttatct cgaatccagc ttttctaaat
     1501 cctgcataat tggctatagc ttgtcctaag ttacctgctc ctacaagaac tgcgttgtat
     1561 ggtcgatcca acccaagaat tttacctatc tcagtatgaa gagcttctac attataacca
     1621 tatccttgtt gtccaaatcc accaaagttg tttaaatctt gtcttatttg agaagcggta
     1681 aaccctatta tatcactcaa ttctttagaa gatattcttt gtatatccct atctaataag
     1741 tctccaagat atctatgata ttttgggagc cttcttataa ctgccattga tatattttta
     1801 tttcccaaca tcttcacctc ctaaaagtgt cctctttttt gtttgattca caataaaata
     1861 tttctttttc aaaactaata catataacct tgattatatt atatcaaaac attataattt
     1921 tagatagttc aaggaggtag aaatttatga ttgttttgtc atgtaataac ttaaataaaa
     1981 gttttggtat agattctata ttagaaaata ttagttttac agtaaatgaa ggcgataaaa
     2041 ttggtataat cggtgttaat ggtactggaa aaactacttt atttaaaata atttcaggaa
     2101 tttatggata tgatagtgga gatatttaca cttctaaaga ttgtgaaatc ggatatttag
     2161 aacaaaatac caatttttat tcggataata ctattttgga agaagtacta gaagttttta
     2221 aaaatttgat agaaatggaa tcctatttaa gagaattgga ggttaaaata tctgaagaaa
     2281 gtacaaaaac aaactctcca attatagaaa aaatcatgga tgaatattca cataaactag
     2341 aattattttc agatttaaat ggatatggat ataaatctga agccaaagga gttctaaagg
     2401 gattaggatt tagtgacaat gatatggata agccaataag catactttct ggtggtgaaa
     2461 aaactagagt tcttttagga aaattacttc ttaagaaacc aactctattg ttacttgatg
     2521 aacctactaa ccacttagac tctgaagcaa tagaatggtt agaagtattt ttaaaacaat
     2581 ataaagggac tgtcatgtta atatctcatg atagatattt cttagaccaa tccgtaaata
     2641 gaatttttga agttcacaac aaaaaactta aagtatataa tggtaactac tctaaatttg
     2701 ttgagttatc taaaatagaa aaagaactag agcttaagaa atttgaagac caacaaaaag
     2761 aaataaaaaa gcaagaagaa tcaattgaaa gactaaaagc ttatggtaga gaaaaacacc
     2821 taaagcgtgc aagaagtaaa gaaaaagcat tagacaaggt agacgtttta gataaaccag
     2881 aagcttatag gaaaaaagct cgtatacaat ttacgccttc tgtccagagt ggtaatgatg
     2941 ttcttcaaat tagagatgta tctatgggtt atggagaaag aatacttttt aaagacttgg
     3001 atttagatat atatagaggg gaaaaagttg cacttatagg tgcaaatggt gttggcaaat
     3061 ctacattatt taaaataata actaatgaac tacaacctct tagtggcaat attaaatttg
     3121 gaactaatgt acatgtttcc tacttccatc aggaacaaaa aactcttaat ttagataaca
     3181 caataattga tgagatttgg gaaaataata ctcatcttac tcaaactact ttgagaaata
     3241 tgttaggggc atttttattt gtagatgaag aagtttttaa gaagatttct acattgagtg
     3301 gtggagaaag agctagagtt gccatactta aacttatact atcaaatgca aacttattgt
     3361 tattagatga gcctactaat cacctagata ttgattctaa ggaagtcctt gaagaggcat
     3421 taactaatta tgatgggact atatttacaa tatcacatga tagatacttt ctaaatactg
     3481 ttgttgataa aattttagtt ttagatgaaa atggaattac tgaatatctg ggaaattatg
     3541 attattatat agataaaaaa aggcaaatcc aagagatgag tattatagaa gaaaaagaag
     3601 aaaaaactag aactcaaata aaagatgaaa aaagaaaaga gagagaacaa agagaaatag
     3661 aaaagaaaaa tagaataaaa agacaaaata tagaaaaaga aattgaaaag ttagagattg
     3721 aaattgaaaa attagatata cttttatgtc aagaagaagt atactctaat ccagataaag
     3781 ctaaagaagt tagccaagaa aaaattaatt tagaaaataa ccttgcctct ctatatgatg
     3841 agtgggaaga atttatgtaa aaaggagagg attatttgca agaactattt tctttaaaag
     3901 acgaaaataa aagattttat aaaatacttc tatctttatg tatacctatt atcatacaga
     3961 acttaatttc tacttctgta aatgtaatag atacaattat gataagtagt ttgggggaaa
     4021 catctgtagc ttcagttgga gttgcaaacc aatttttctt tctttttaat atgtccttat
     4081 ctggtatcac aggaggtgct ggagttttta tttcacagtt ttatggaaag aaggatgtga
     4141 gtaatataag aaaagtcaca ggtcttactt gtgtattggc aattgtttta agttttgtat
     4201 ttgttattcc tgctttacta acaccaaagc caattataca catattttcg tatgattctg
     4261 aggtggtaaa actttgtata gattacttta gtattgcagt atttagttat ccattaattg
     4321 ctgtaagtac tgtatttagc actggttcaa gaggtgttag aaatcctaag ttaggtatga
     4381 tttgtagtgc atttgcactt gtaacaaatg ttattttaaa ttatggattt atatttggta
     4441 acttcggtct tcctgcattg ggtgtaaaag gtgctgctct agctactgtt attgctagaa
     4501 tatgtgaatt aattttaatg attacttatg tttaccttta taaaaaagac tatatattaa
     4561 agtttggtct aaaaaatcta aaagctattg ataaaatttt tataaaatct ttttcatcaa
     4621 aaagctttcc tatatttgta aatgactctg tatgggcaat tggaactgtc ctgtattctg
     4681 ttgcatatgc aagagctggt acttctgcta tagcagctag ccagatagca accagtacag
     4741 gtaatttctt cataatgaca gctgtatgta ttgcatctgg tgcatctatt atgcttggaa
     4801 atgaacttgg agctgaccat attaaaagag ccattgaata tgctaaaaag ttttctatac
     4861 ttgtattttc agctggttta atacttggaa ttattttaat tttaaatata ccattattat
     4921 taaaaatgtt tagtgtttct gatagcttag cttctgatat aactaagata ttctttataa
     4981 tgggaatact aatggctctt aaatctttta atacattggt tataattggt attttaagaa
     5041 gtggaggaga tactaaatat gctctatttt tagaattagg atgtatgtgg ttagcttcta
     5101 ttcctctaac ttttatagct gcatttaaag gtgcacctat atttgtactc gttttgctca
     5161 cttatagtga agaagtggtt aagtttatat ttggtgttcc tagagcatta tctaaaaaat
     5221 gggctattaa tatagtaaag gaaattgatt aataattata ctatacatta taagtataca
     5281 atatttctaa acagtctcat tgaaaataac taaagtattt ttgattttca gtgagactat
     5341 ttctattttt tacagaaatt attatacttt attttgatta agtaacaatt tttactcatc
     5401 ttccaaaaat tcttttactt caaatgccaa tttatgtaat tcatcatagt ttaaaatttc
     5461 atatgtatta cccatttttt ttactatctc cttttcagtt aactttagca gagaatggta
     5521 tctacttttt ctactaattg aatagatttc actgaaatta cgaatagctt ttatattgca
     5581 gacattattt tcttgtttat ttgatataca ataggcaata acttctgtgc aagaaaatag
     5641 ttctcttacc attctggttt tatataattc atatattctt cttgtacatt ttctgtgaaa
     5701 tagtgcaata aaatctggtt tgagataaaa ctcttcaaat acattttttt caaatcgaaa
     5761 taattttata ttggtctttg caaatatatc acatttcaaa ttatgttcat atatgtaact
     5821 aattttgcct ataaattcat tttcaggtac aatgtcaaca agaaatttat ttcctcctct
     5881 agtagtacac tccactaatg caactccctc tattataaag aacacttctt gtaagtcttt
     5941 atctgatgct acaaatcttt gacccttttt atattcaaca attgatattt tgtttaaatt
     6001 atttttaata attggatata tacttgtttt ttctgctttc atcaaagtta cctcctttac
     6061 aatatattgt ataaaatatt ttatttatat gttttaattt atattatttt taataaaaga
     6121 taaaattaca actaaattaa acttgacaaa ttcattctac tgacgcagaa taaacttata
     6181 catatatatt ttaatatata atttatttta atataattct atatttacaa aattatatta
     6241 tcaatagaat ttattaaaat aaattttttt gggtgtatat tataatttaa tcgaatagaa
     6301 atgaaaaata tacatacagt ttaagtttta agcaacaatt agggggattt ggtgacaaga
     6361 tttttaatta taagaaagaa agtgtcttaa tgcaactttt ataaagtcat tatagacact
     6421 ttctttttta tttgataaat ttaaattatc aaaattatat tgctttatac aactattttg
     6481 ttggaaacta tttattatat aaggagaaat aaatgaaaaa atcagtagct ctggtaaatg
     6541 atagtagaaa agatttaata gactttttag aaaataattt gaaattagtt tttggagatt
     6601 ctataaatat taatagatac ttcataaatg aaataaatga taatgatatt ataaatgatg
     6661 atgtgatatt agtaatgtca gtagaaagat tagacaaaat tataaataat atactagata
     6721 agaaaaaagt gattgtggta agaagaactt ttagggaaga taaaatttat aatctactat
     6781 ctttaccaca gggtacaaat gttctaatcg taaatgattc tgatgaaact actctagaaa
     6841 ctataagttt attttataaa attggagtga ctaatattag accaattcca tatatgaatg
     6901 ataataacta taaaaatata aaaatagcta taactccagg agttccagaa aaagtaccta
     6961 gctttatctc agatatattt gatttaggtc acagatatat agatatttct acatttatag
     7021 aaataataaa tttattacaa attgattcaa aggaaataca atctaatcta gttaaatact
     7081 cagaagaaat aataagttta gatactggta taaaagataa atataaggaa ttatttttaa
     7141 aaatagaaga attagataca atattgaatt tatcaaaaga tggtatatta tttacttcaa
     7201 aagatggtga aataaataca tataatagta aagtaaaaga tattttagac ataaatgagg
     7261 atatatatgg aaaatacatt gaggatatat ttgtagatag tttaaaagtt ttattgagtg
     7321 agaaagagat actagataaa gttgtcgtat tcaataagaa atatataaat gtaaataaaa
     7381 aaaacatata caatagagat gaaaaaatgg gcacatatta tagtttacaa gaaataacat
     7441 acataaaaaa attagaacaa aatctcacta aaaaattaag agaaaaagga caaatagcaa
     7501 aatatacatt taaggatata aaaacaaata gtcctaaaat gtttgaatgt atagatttag
     7561 ctaagaaggt ttctaaatct gacttaagca tacttatcag aggtgaaagt ggaactggta
     7621 aagaactcat agctcagtct atacataaca actcaaatag aaaaaatcaa ccctttatag
     7681 cagtaaattg tgcagccgtt cctgaaaatt tattagaaag ccagctattc ggatatgaca
     7741 agggaacttt tactggaggt ctaaaagatg gaaaacaagg attatttgaa cttgctaata
     7801 atggaacaat attcttagat gaaataggcg atatgccttt agaattacaa acaaaactac
     7861 ttagagttct tcaagaaaag cagataatgc ctgttgggtc tcacaatgta attaatatag
     7921 atgttagaat catatctgct acaaataaga atttagaaca aatgattgat aatagccaat
     7981 ttagagagga tttatattat cgattaaata caattccaat aaatatacct cctctaagag
     8041 aaagaaaaga agacatatta attattatgg aagatttaat taataagaaa ttagtaatca
     8101 caccagaagc aaaaaaactt atacaaaact atatgtggaa aggtaatata agagaactcc
     8161 aaaatgttac atcctattta aacattatgt gtgaagatat tgtattagag aaagatttac
     8221 ctccaaactt aaggtcttca gataacaaaa atacatcatt aaaactaaaa tatagtaaga
     8281 atgatatatt gaatatatta gagatactaa ttttaaataa ggaatcagat gttggtattg
     8341 gtagaggatt aattttaaaa gcactactcg ataagaacct tcaaattaca gaaggaaaaa
     8401 ttaaaaaaat atttgaatat ctaaaaaaag aagaacttat aatttgtagt tcaggaagat
     8461 atggaagtaa aataactcaa aaaggagaag atttttacaa taaattaaaa tataaaggtc
     8521 tataaaatta taatttatca aacaaagaaa tctattcagg attaaagtac tccttcaata
     8581 ggttttttta tttttcaagt accctgaaat tataatttat tttgtacata aataatagga
     8641 attaatagga ataggataat ttttttattt cctatttttt ttatataaac tagcatataa
     8701 aatatatttt cttcaaaata atatttttta ctgaaaattg aaactatgca atcatttttt
     8761 atactataca tatagataaa tgattatatt tattcaatat tcttataaaa aacaaagttc
     8821 tccatcgaaa attatggcat aataattgct tattatatta ataaaacaat aaaaagcaat
     8881 tggaggaata aaaatgcaac aggctttgaa attaaaatat caaaccaaag aattaggaaa
     8941 agattttgta atagaaagtt gtaattctat aaaaccatgg cttataaaca taagaagaga
     9001 attacataaa attccagaat tagctcttga agaaaatctt actaaacaga aggtaatttc
     9061 ttatttaaaa gaaatcggaa ttgattatat ggaatttaca aaacacaatg gaattatggc
     9121 atatatttta aaagaaagtg cagataaaac gatttgtata agagcagata tggatgctct
     9181 tcctattgaa gaagaaaaca acatacctta taaatcaata cattctggaa aaatgcatgc
     9241 ttgtggccat gatgcacata caaccatgct acttggagca tgtaaagttt tacattcaat
     9301 aaaagataaa cttaatgtaa atgtaaaatt cttatttcaa ccagcagaag aaggttttgg
     9361 aggagcaaaa ttcttagttg aagatggatg tttagaaaat cctaaggtcg attatatatt
     9421 cggacttcat gttatgccac atatcgaaac aggatttatc gaaataaaat atgatacatt
     9481 aaatgctagt gtagacacta taaaaataag tatcaaaggt aaaagagctc atggtgcata
     9541 tcctgaaaat ggtattgatg caatagttac agcttcacaa attgttacat cattacaaac
     9601 tataataagt agaaacttag aaccaaataa tgctgctgtt ttgactatag gtaaaatata
     9661 tggtggtgat gcacacaatg taatatgtga agatgtaaaa ctagaaggca ctttaagaac
     9721 cttaaatagc aaaacaagaa actttatgat tgataaaata gctaaaattg ttggagacac
     9781 tgcatctgca tttggttgtg ttgggacttt acatgtaagt gatgaaaact atcctgctgt
     9841 tataaatgaa aaagaattag tagatacagt tatatcaaat actaaagaat tacttggaga
     9901 ggaaaaattt atattgagac caaacccgtc tttaggtggt gaagactttt ctttttacac
     9961 agaacattgt aaaggagcat tcttccattt aggatgtaaa aatgaggaaa agggcttaat
    10021 ttcacctctt catacctcta gctttaatat agatgaagat tgtttaccaa taggtgttat
    10081 gatgcatgta atgaatactt tgtattttaa ttagtacagt aattaataaa agaataggag
    10141 ataaattaat atggaaaaag ctaagaaatt aggtctattt agtatgatat tattagggat
    10201 taatagcata ataggttcag gtatattctt actaccaggt aaagtttata atctagcagg
    10261 tcaaaacagt atgtttatat acatttttgc tacactctta gttctatcaa tacttttatg
    10321 ttttgcagaa gttggaagca tgtttgataa gaatggagga gcatatctat actccaaaaa
    10381 agcttttgga gattttatag gatttgaagt tggtacaatg tcttgggtta ttagaattat
    10441 atcatggtct actctagcag taggatttgc aacagcttta ggttcatttt ggccagaatc
    10501 tgccactgaa tacaaaggtt atatagctgc aatactcgta acactacttt caataaatag
    10561 cttatttgga attaaaagca caaagataat gaataatgta ataactatag ctaaattagt
    10621 accattaata gtttttataa tagttggaat attctttata aaatttgtaa atattgttcc
    10681 ttcaggaaat gtagttaact ctagtatggg tccagccatt atattagtat tttacgcatt
    10741 taccggcttt gaatcattta ttgtggcaag tggagaaatg gaaaatccta aaaaaaatct
    10801 tccagtcgca ttaataacca caatatttat atgtgcaata atttatatat taattcaaat
    10861 tgtctgtatg ggtatacttg gagatagact atttgaaaac agtattccaa ttgcagatgc
    10921 ttcaagtgta tttttaggca attatggtaa atttttcata tctgtagcaa cattaatatc
    10981 tatatttggt ataaatatag gttcatcaat agtaactcct aaatgtggtt cttctctagc
    11041 agaagaaggt tctcttcctg catttatagg aaaaacaaat aaatatggtg ctccatatgt
    11101 agcaataata atatctttaa tttgttgtat accacttgtg ctaactggaa gttttgaaca
    11161 acttgcagta atgagtgtaa ttgcaagatt tgctcagtac ataccaactt gtttatcagt
    11221 aatagtttta agaaaaagaa cagatgttaa ggcctctttt aagattccat ttggtcctgt
    11281 tataccaatg gtagctattt taggtagctt atggttgtta caacaagctt gggcagaaga
    11341 tatttcaaaa cctataactc aaaatagagt tttaatagga ttaggggcaa tgttgttaat
    11401 agctcctcta tatatattta tgaaaaaaaa taaggaaatc gagcataaaa ataaatcaga
    11461 agattctaaa ttatatattt cgagagaaaa aattcaataa tattttacaa actatttaaa
    11521 aattattttt ctaatactat agaaataatt agaaatggag atgttacgta tctaaaagta
    11581 gttgataaca cggatattgg tacttttata gatatgggac ttacaaaaga catactagtt
    11641 ccttttaaag ctaagactta tccattgttt agagatgaaa aatatttatt ctatatatac
    11701 ttggataaaa gcggaagaat agctgctact acagacatag attcatattt agaaacagac
    11761 catacttaca atgtaggaga tgttgtaaat ggagttgtat atggattcca aaccaacaac
    11821 tctgctatga tatgtgttga caataaatat gctggtgtaa tccttcataa tgaatacttt
    11881 acagaattaa aagctgggga tgttcttgaa aatttacatg ttataaaaat atacgatgat
    11941 ggtaaattag gactttctcc tagaggaaac agaaaagatg aacttgatac cttagaaaat
    12001 aagattttaa gctatttaga aggttcagat ggatatatga gattcaatga taaatctgat
    12061 cctaaagata tttctatctt atttaattca agtaagaaaa ttttaaacgt gctcttggag
    12121 tacttatgaa gaaagactta atttatcaag atgaagatgg aacttatcta aagtaaatta
    12181 tataacacaa aaatcgaagt aaattatgaa agatattttc tatttcatat ttacttcgat
    12241 tttttattta aattactacc tctatttaaa ttactactta tatttattgt gcatttacta
    12301 cctctattat gtctaattca caaatctttt taattggttt tcttactgca agcaaagtta
    12361 ttattatcat agttactaca atgataaaca gtaaatcaaa aggtatttgc cagtctatat
    12421 ggaattttaa tgttactaag gatacaaata tatatctatg taatattaat ccaagtacac
    12481 tccccaataa acagccactt acagcatatg tacttgattc tactattacc atttttctta
    12541 attgcttatt ggacatgcct attgcacgca taataccata gtaatttatt ctacttgtaa
    12601 cgcttatatt catactgttt atgatattaa acatacttat aatcgcaatt ataaatacaa
    12661 acccatatac aaaaactgcc atagtgtaaa atgaattttt agcctctaag ttaccttgtc
    12721 gcttatctcg aacctctaaa ttatcatcta gaacatttct tatattttgg attaaatctt
    12781 caggagcatc ttcatcaact tgagtatcaa ttattatata gtcttttata tctgttatac
    12841 tagtaaaagt attttcagat gttataatat ttccaatata tttgtcttta acatcaaatg
    12901 aaattgaaga aagtatacct gctacctcta tctctttctt agaatttcca tttttaatat
    12961 atatcttatc tccaatatta aacccatgac cttcttctac caatccgata ttttctgact
    13021 ctaatttatc ttcattgatt tcccctttta ctagatactt tttggcccag tcaaattgat
    13081 ttttttcata agaaattagc tgagatgttg cctccttatt attgtatgta gcctctacat
    13141 ccgtcatcat tctactgtat acttttttta ttcctttgat attacttatt tcattaatat
    13201 cgtctttagt aagaacagac ttaaagtcag cgtcaacaat agatatatga gcagcttctg
    13261 gttttatagg tttaagtgcc tgatacataa agtctataaa tacactaaaa cctaaaaaca
    13321 atacaatact aatagcaaat gaagaagtca taagtataaa agattttttt cttgaaaagg
    13381 catgatttat tcccatagaa acatcaattt tagaacttcc tgcgttagca attttacttg
    13441 cttttccact agttaaaatt tctttgaaca ttagattacc acgtatagca cttagtggag
    13501 acaccccaga agcttttttt gctggaacaa tagctgataa tattactgtt aaaaatccta
    13561 ctattattcc aaacacaata ccaataaagc taacctttaa aattggcatc gaaccaaata
    13621 actctgtatt cacatatttg agaaatatag aggatataat ggttacaata catcctaata
    13681 acaatcctag tggaatagcc tttacactta gaattaaact ttctaaaaca acaaattttt
    13741 tgacttggag ttttgatgct cccaagcaac gtaatagtcc aaaaaacttt attctctcta
    13801 ttacacccat attaaagcta ttatatatca tgataatact tgctattaac acaagtataa
    13861 aaagtcctcc tgctgttaag taaagtggtt tagaataatc agagtcacct tgacctataa
    13921 tagaaagtaa tttttcattt ttaccaattt gtttatcact taaattgaag gtattcttaa
    13981 tatcagaagt agcttttcta atattaacct tgtctttaaa tgtaatataa taataatcat
    14041 atgtttctga ttttttgata gcttgtgtac ctaatattga aagcattaat ccatgcttat
    14101 ctgaggctct taatcttgat aatgatgaat attcacctac tatcttataa tttttatttc
    14161 taccatctgc taattttatt ttgataaaat ctcctacttt tagattaaag cttttaactg
    14221 catctacatc tattaaagtc tcattctcca caacaggata acgacctttt attatttcta
    14281 gacctaattg ttttgctaaa tcttcatcta aaccttgtat caaaatctcc tttgatttaa
    14341 actctccatt atcaatgact tgtcctctac catagtgagc tatatctact ctactactta
    14401 tttctgatat agcttcttca ttaagatttt taagtactat gtgccaattt ccatcatctt
    14461 ttataaattg tactttttga cattgtatag ccatgtctgc tagtccaaaa atagcagtaa
    14521 ctaaacatac tgcaatgaca atacataaaa ttgtagttct attttttttc ttatgtacct
    14581 ttgcatattg tggtactagt tctaaatagc ttctcattct ctattcaccc ctaggtcttt
    14641 aagcattcca tcagaaacct gtagtatcct atctgctgta gaagctatat ttgtattatg
    14701 agttatcata ataatagttt ggttatattt ttctgctgtc acttttaaaa gtgaaattac
    14761 atcactgcta tttttactgt caagatttcc tgttggctca tctgccaata taattgatgg
    14821 ctttgtaatc aatgctcttc caattgcaac cctttgttgc tgaccaccag ataattgatt
    14881 tggaagatga ctttttctat ctttaagacc taaaattgtc aatacttcat ctagataaat
    14941 agtgtctggt tttttgtaat ctagtagtaa aggaaatatt atattttgtt ccacagttaa
    15001 ttcgggtata agattatatg attgaaagat aaaacctata ttacgacgtc gaaaaatagt
    15061 ctgtttttct tctttcattg aacatatttc tttaccatca acaaatattt ttcctgaggt
    15121 tggagtatca agacctccta aaagatttaa taatgtactc ttacctgagc cagattctcc
    15181 aactacagta acaaactctc cctttgaaac tgtaaatgat acatctttta atgcctctac
    15241 ttttgtatat ccatttccat atgttttaca taaattatta accacaagaa tatccatttg
    15301 ctctatctcc tttctgatga ttaatataag aataattcat acaccttact gtaagatgta
    15361 tcctatctta catattagta agctttaaaa aacttagagt aaatgtactt ccttgtaaaa
    15421 aattactttc tacagttatg gttccattgt gctgttctat gatagattta actagtggca
    15481 accctaatcc aaggccttga ttactttttg aaaacttact cctataaaat cttttaaata
    15541 tatgatgtat atcttcaata tgaattccat ttccggtatc actcactgaa ataatagttg
    15601 ttataggtgt ttcaacccat tctatcatta ttttatcgtt ctcttttgta ttatctaatg
    15661 cattttttat aaggttgctt aaagcttctg atatccattc ataatcgcaa aacaagtttg
    15721 tattttctag acctcttata gagatactct ttccttcttt ttctgctcta gtttcaaaac
    15781 tatataaaat tttagtaatc aactttttta cgttaacact agctttgtta agaattattg
    15841 tctttgaatc tatctttgta atctttaaca agttttgtat caatgattct atacgctcta
    15901 attcattttg actcttctgc acaaaattct caacaatttc actattatga ctctcttcct
    15961 gcaatatttg attatacatc atcaatgctg taagtggagt ttttagttga tgtgatatat
    16021 ctgaaattgt atttttaaga aattctttat tctgcttttc tttcaatata tgtgtgttta
    16081 gagatgttga catagcatct attgaaccaa ataactttga taaactacct tcttcataag
    16141 tatttaaccg tacatttata tttccattca taaatgaatc aattttataa ctagcttctt
    16201 ctatcttgtt ctgttgccta aaaaaataca catacaatat aaaaataatc atagtaaaga
    16261 acacaaataa atacaaaaac atagttttta tatatttacc ttgaaacaat ttcacacttg
    16321 gcataaaact atcatctgta ccaaagtcat atctagctgt atttaagatt tcttttcctt
    16381 ttaaagtata tttatcttca ttagtagtaa atactgctgg tatttttgat ttatcaacac
    16441 ccatactatt taagtatcca gctatattat aatcatgttc taacagtgca cttttataat
    16501 cattagttat atttataact actatttgac ctactactat aaacatcaat aaaacggata
    16561 taatcaccaa aaaaaatatt tttatgtctt tattagaaaa taccttcata ttaacaccct
    16621 cactttataa cattccattt gtaacccatt cttctaactg ttaatagatt ttttggattt
    16681 tcaggattat cttcaatctt atttcttaac cttcttacat atactgacag agtattatca
    16741 tctacaaaac taccatttcc atcccataac ttatccaata tattttttct tgtaagtaca
    16801 atatttttat ttttcataag taagcataat aatttatatt ctgctgtagt aagttcaagc
    16861 tcaatttcat ttttaaaaac acgattttca agtaatttaa cagtaatatt atttgatttt
    16921 agttcagtta cattttcact tgtaaaatta taacgtctaa gtaaagcttt tattcttgaa
    16981 attaactcat ttaatttaaa aggttttgtt atgtaatcat ctccacccat atcaagtccc
    17041 atcactacat ttacttcttc atcagaagca gttaaaaata tgattggtac attagaagct
    17101 tctcttaccc ttttacaaat atcaaaacca tcaccatcag gtaaggatac atccaaaagc
    17161 agtaaatcat aatttttttc tttataaatt tgcaatgctt cttctactgt tcttgctaca
    17221 tcaacactaa atccattctt ttgtatagaa tattctaatc catatatcaa acttaaatca
    17281 tcttcaacca acaatatact actcatctca atcacttcca tttttttatt ttttacttta
    17341 tatatattat atacctaact tattatattt ttactctttt aaaaatacaa taccttgaaa
    17401 tcaatgattt aaatatttta aaattatttt tatatgattt tatatagtta atttaatact
    17461 ttgaaatctt aataataaaa aaagcttaaa atacactaag tattctaagc tttattcaat
    17521 cacattttat atatttatat tatttatttt tttaaaatac tattttcaat attgcctcta
    17581 ttaaacattt cattatcatt atctaaagag atatcactta tatttctaac agttgttgaa
    17641 tagttaccac taagttctat ctcactgaaa tgcatatgca ctatcttttg tattacaaat
    17701 cccattgatg caagtaaaat agcaaatatt atatactgac taaagttact catataataa
    17761 tttataatgg tatattcatt tcctttgact gttaaatgtt ttgtagatag catagtatat
    17821 atatattcag tacaatttat aaatacatat ataccataaa tagctaacac tacagataaa
    17881 caatacattg tgattgaaat ctttgatatt tttttaaact tactatcttt acaacactta
    17941 atatttgtta tttatcataa acaaggttat tgtattatca ataattttta ggttacactt
    18001 attattagtc ttgtgttaaa acgacagtag acttaagagt agagttttat taagccaata
    18061 tttatatttg ctatacattg tacccctatt acatcataca tataacttgg ataacttatt
    18121 ttatttttat ttctaaatta tttaaaatca atatctacac cattatcgat aattgcaatt
    18181 aaattcatat aaaacctctt taatttatag gctgttcact ataaagtctt tatttcttcc
    18241 ataaaatttt cattttgaat tttattcaat gtttttagtg tagttatcat gattatagat
    18301 actaaaataa atactattag tataatagcc cctataaatt tccaatttat taaattgata
    18361 ctttcaaaag gttttattat tatacttctt acaattaaaa taggtattat agatatacaa
    18421 aagcttacta taatatatat ataactttcc aatttaacaa ttttatttat ttcatgtttt
    18481 gatattccta ttcctctaaa tagtgcaaat tccctacttc tgctattaat actagtaact
    18541 atagtattgc aacaattaaa taaagataat ataactataa ttccagttac taataccata
    18601 attagtgttt tttgattatc aattttttca tattcagctg tttcagcttt gtatggaatt
    18661 aaaattcctt tatttgtttt ttctgttatt ttgtttaact cactttcaac atatttatca
    18721 ttggctagtt tactcgtcca tatgtcaaat ctttcataag atgatattcc tgtaaactta
    18781 ttaaattgat ttgttgatat tactccgcca aattcagctc ccacttgtga ggttactggt
    18841 aatgtagtta taattccaca tactataaat tcttgagata ttgttttggt aaaattattt
    18901 gtttttgaat ctataatatc aaagtatact tttattctat ctcctttttt tatattaagc
    18961 gaatcactgg atttctttgt tatatacaga tatggttgtt tactaaattt atctatattt
    19021 tcctttccat ttatcaagaa atcctctagt atatctgtat cttctattcc taaaaattca
    19081 aaagaattat tatatacctt aattccatct tttacttctg atctagcttc aatattatct
    19141 ttattttttt tccaataatc agagttctta tttatacttt tatcatcaat ttttatatta
    19201 aaccacttat gcctatatga gtttactttt gttacaccat caattgcctt tatctcttgt
    19261 agtacatttt tatcataacc tttattattt gaacgtatag tacttatatc aatattatgt
    19321 aataaataat ctccttgtat ccaatatttt aatacaccat cacttaaaaa gttactagta
    19381 gactttgtgt atattgttgc cattaagaaa aatataatta ccattgatgc tatagataaa
    19441 tttgtacgtt tcttatttct ttgtatattt tttgatgcta tgttaccata gtctttgaaa
    19501 agttttatca taaatttatt tataaaggaa ttttgtttta tatccacttt ttcattattt
    19561 cttcttatac aaatcattgg atcagtttta actgataaca taagaggttt tgaaactgat
    19621 attattatag ttcctaaact taataatata gaaattatgt atgtgtttat atagttctgg
    19681 ttttttatag aatttatata gttgacattg tatattactc ctataacata ctcccttgat
    19741 aaactagcta ttatcccacc taaaattaga cctatagaaa tacttataat agagtatata
    19801 gatacttcta aaatcattat tttaaaaata tcatttggat taaaccctaa agccctaaat
    19861 atcccaaaat cctttattct atttgatact aatatataaa aaatattata aattaataat
    19921 atagttgcta gtgctagtac tatattaact attatataag gtatttttaa attcatacta
    19981 tcagatatag ctattaataa ttcattatta aaataaatgt tattctcatt caaatttcca
    20041 tttttaataa gtttttgtga ttgtctctgt atattttttt cctttttaaa agtaaacata
    20101 acttgatcat atttattttt agtaggaata ttcttgatag cacaatcgtt acttataagg
    20161 ccaattgatg ttccttgagc ttttaaaata ggattactat ttaagatacc agttatttta
    20221 aattcttttt ccccagtata taaatcttct tgatctatcc cttgtcttct ataatctaat
    20281 gttatccttt ttcctatagg gttttttatc tttttttgtt taatatacca tgaatctata
    20341 gcgatttcat tttcagctac tggtaattta cctttttcta gtgtgtaaat atcatttagt
    20401 tcttcagcaa ctttatcctg agatagaatt tgtagtgttt gacctttacc aatatcacta
    20461 attcctaaaa gcattgattt tccaacttta tctatacttg tgttattttc tatatatttt
    20521 atttcttcgt cttttggatt gatatattgt gcatgtttta atgtaaattc atttttagct
    20581 tgatttatca ttaagtttgt cgcatcttca cttattatat tcataattaa aaacaacgct
    20641 gttgaaatta caatacttac aattattaat aatgtatttt tcttatttcc cattatatat
    20701 ttttttacaa tataattaag tttcataaat ctacctactc tcttataaat ccatcttcta
    20761 tttctataac tctatcagca tattttgtta tatctatatt atgtgttatc attattattg
    20821 tctgattata ttttttttga gattctttta ataaacttaa tacatctgac tctgtctttg
    20881 tgtctaaatt tccagtaggt tcatcagcaa atacaattga tggtttattt acaagagccc
    20941 ttgctattgc tactctttgt tgttgcccac ctgataattg atttggcaaa tacattcttc
    21001 tatcttttat tcctagtaac tccatcaatt cattaatata agcttcatct ttatttttta
    21061 tatttatagt tggtagtttt atattttctt caacagttaa aactggaatc aaattatatg
    21121 attgaaatat taatcccaac ttttctttac gtaatgcaga tcttttcttt tcatcaagag
    21181 atgatatatt tatattatca ataatcacat caccatttgt tggagtttca agtcctgcca
    21241 ttatgtgtag taaagtactt ttcccacttc cacttttacc tactatcaca acaaattctc
    21301 cactatttat ttttagattt atttgactta acgctttcac ctctgtttcc gatttaccat
    21361 atattttatt taggtttttt atctctaaaa tactcataat ttactccttg tcttacatgt
    21421 tatttacact tataaattcg atttttgcta tggattttgc aatctctatc tatacttatt
    21481 gatatgtagc aacagaaact atttttataa ttttcaaaaa attcttccgc ctattcactc
    21541 catatagtgc ctttagttat cataccatat cttataattg aattttattt cttatatctc
    21601 ttttaccaac ctttttattt aggaaaagtt cttcaccatg aaattgtaca gtatatacct
    21661 atttctttga ttatataatt aagcttttta tttagtaacc tattttctac atcctaactt
    21721 atatttcctg tttcctcatt ttcatattta taacaatagt tacaatctaa attactactt
    21781 ttagttaccc agactttact ttacatgctt tgcctctaaa ctgctgatgt taatatcttc
    21841 ttaatttttt cacacctatc ttcaaatata aaatctttat ctaaaagatt attgtattca
    21901 gctagacata accaacaaaa tgatttcatt aaatattctt tacattcatt attacataac
    21961 tttaagttga aaaactcatt atatccaaaa ttcgttgata cccatattat taatataact
    22021 tacaatgtct ttttgaatat ctaaaaaaat acttttataa cttattttat atatcataca
    22081 ctatattatt gctttcataa tctataaagg tttcatatga tacaaacttc taaggtggga
    22141 tttttggtct ttaataacga ttataataat atttatttat atttctgatt taaacattat
    22201 tttacttgaa atgtttatta aataaatgaa t
//
LOCUS       DAMJZE010000045        22215 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJZE010000045 DAMJZE010000000
VERSION     DAMJZE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.045"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            451..1521
                     /locus_tag="PC541_001580"
     CDS             451..1521
                     /locus_tag="PC541_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839047.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1505..2374
                     /locus_tag="PC541_001581"
     CDS             1505..2374
                     /locus_tag="PC541_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2839048.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2418..3278
                     /locus_tag="PC541_001582"
     CDS             2418..3278
                     /locus_tag="PC541_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2839049.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3309..4613
                     /locus_tag="PC541_001583"
     CDS             3309..4613
                     /locus_tag="PC541_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2839050.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4676..5938
                     /locus_tag="PC541_001584"
     CDS             4676..5938
                     /locus_tag="PC541_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2839051.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6125..8095
                     /gene="uvrB"
                     /locus_tag="PC541_001585"
     CDS             6125..8095
                     /gene="uvrB"
                     /locus_tag="PC541_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2839052.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDNYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8114..10939
                     /gene="uvrA"
                     /locus_tag="PC541_001586"
     CDS             8114..10939
                     /gene="uvrA"
                     /locus_tag="PC541_001586"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2839053.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11198..13015
                     /gene="uvrC"
                     /locus_tag="PC541_001587"
     CDS             11198..13015
                     /gene="uvrC"
                     /locus_tag="PC541_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2839054.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13015..13944
                     /locus_tag="PC541_001588"
     CDS             13015..13944
                     /locus_tag="PC541_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2839055.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14156..15952
                     /locus_tag="PC541_001589"
     CDS             14156..15952
                     /locus_tag="PC541_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2839056.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16092..17873)
                     /locus_tag="PC541_001590"
     CDS             complement(16092..17873)
                     /locus_tag="PC541_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2839057.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17896..19782)
                     /locus_tag="PC541_001591"
     CDS             complement(17896..19782)
                     /locus_tag="PC541_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2839058.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19799..20296)
                     /locus_tag="PC541_001592"
     CDS             complement(19799..20296)
                     /locus_tag="PC541_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2839059.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20614..22095)
                     /gene="cls"
                     /locus_tag="PC541_001593"
     CDS             complement(20614..22095)
                     /gene="cls"
                     /locus_tag="PC541_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2839060.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8555 a         2974 c         3699 g         6987 t
ORIGIN      
        1 ttttttgggt aaatttataa aaattaaaaa atttttattt tttatgttta aaccttgtaa
       61 atttgggaca taatataatt gtaaaactta atatagcaaa aataaattac tcaatctccc
      121 ccacttaaag acctctatcc cccatagagg tcttttttac tttcaaacca aattatataa
      181 aaaataaata gttttatatg actcttcata aattaaaatt acattgtgat agcaaaccat
      241 gaatatgaaa aatatagaaa attatagcta acataagctg tatatcatct taacttcagt
      301 atatttaaca aaaaatgttt ataatttaac ctttatttta ataagtttac catatattca
      361 ctttgtctta atatatcctt tacaaattaa gagtattatt aatctataag taaatcgtag
      421 aattaaatta aatattttgg gaggataaaa atggcagata taagactaga aaatgtaaca
      481 aaatcttata ataaaaaaaa tacagttata gaaaatttaa atcttactat agaagatggt
      541 tcttttacag tattggtagg cccaagtgga tgtggtaaat ctactacgtt aagaatgatt
      601 gcaggtcttg aagatataac ttcaggtagg ttgaagattg aagaaaatga tgttacaaaa
      661 acggatgcta gtaaaaggga tatagctatg gtatttcaaa attatgcatt atatccacat
      721 atgactgtta gagaaaacat agaatttggt cttaaaaaca aaaaagtaga aaaagaaaaa
      781 agagaaaaac ttataacaga agttattgat gtagtaggat tgagagaata tttaaatgtg
      841 aaaccaggca acttatctgg aggtcaaaga caaagagtgg cacttgctag agctatggtt
      901 aaaaatccaa aggtattttt aatggatgaa ccattgtcaa atttagatgc taaattaaga
      961 aatcaaatga gaacagaatt aatacaatta catcaaaaac taaagtctac atttgtttat
     1021 gtaactcatg accaggttga ggcaatgtca atggcagata agatagttat aatgaacaaa
     1081 ggaaagataa tgcaaatagg ttctccaaag aagatatatc aaaatcctaa aaatttattt
     1141 gtagctcaat ttatagggag tccttgtatg aacatactag atataaaaaa tgatattaaa
     1201 tttggattca gaccagaaaa agcaacacta gagagtataa acatagatgg gaaaaatgat
     1261 gatgtattta gtctatttgg aaaagttgta actaaagaag tgttgggaaa tgaaactata
     1321 tattgtttat ctgttcttaa tagagagata agagtcaaaa cggaaaatga tatatttgat
     1381 attggaagaa ctttaagaat aagtgtatta gaaaaagatt tatattattt tgataaaaat
     1441 ggagaaagaa tagaagatgt aaaagtatgt aaaagtatgt attctaaaat caaaggagat
     1501 atttatgaaa aagcaattta ataaagcata tttatatata acacctgcta ttataggaac
     1561 tttattgttt atagtctatc caataattaa gacgatatct ctaagcttca aatctggtag
     1621 tcttcttagt tcaacctttg aaaatgtagg cttaaacaat tatacagaac ttttaaagaa
     1681 tccagagttt atacaaacta taacaaacac tgcaatttat acttttttta tggtgactat
     1741 atctatatca ctagccatag tacttgctgt ttggctaaat aaaaattcta taatacacaa
     1801 tttaacgcaa agtataatct ttacaccaca tgtaatttca ctagtatctg tagctgtatt
     1861 gtggctttgg attatggagc cacaatatgg ctttttaaac tggatactat cgacattaca
     1921 tttaccaaca tcaaagtggc tttcaagtga aagttcagct ttaatgtcac taattctaat
     1981 aggaatatgg aagactcttg gatataatat cttaatagtg ttatcaggtc ttaaatccat
     2041 accaaactat atatatgaag catctaaatt agataatgca aacaaaataa cgaatttctt
     2101 taaagtgaca ttgccactat tatcaccaac aatatttttt cttatgataa caacaacaac
     2161 tgcatctttt caagtatttg atgagataag tataatgaca caaggtgggc cagtgggttc
     2221 aactaacatg ttatcttatt atatatatga gtctggattt atatactatg acatagggca
     2281 agcgagtgca gcgagtatgt ttttgttagc tatagttatg attgctacat atgctaactt
     2341 tagactctta tcaaagaaag ttcattatca ataaagaaaa agtagatatt ataaggttta
     2401 tccaaaagga ggagacaatg gatattatac aacctatcaa aaaagacaaa gttgatataa
     2461 aaaaaatagc atcaaaatca atagaaatat taattctatt acttttatct gcaatattta
     2521 tattcccttt tatatggatg ctatctacat cctttaaaaa tccacaagaa atgatgcaat
     2581 taccgcccac tattatacca aatgatatag tgttagaaaa ttatagtaca gcatggcatt
     2641 caggtccatt ttttagatat ttgctaaaca gtgtatttgt aacaggaagt gttttaattg
     2701 tacaattttt agttatggtt ccagcagcat atgcattttc taagattaaa tttaaaggtg
     2761 aaaaaatgct atttgggctt ttgcttgtag gacttatgat tccaccacaa gtaacttttc
     2821 taccagtcta tatgatgatg agtaagtttg gatttataaa tacttatgtt ccacttataa
     2881 ttccatttat gacatcttca tttgggatat ttctgcttag acagaatttt atgcagattt
     2941 cagatgaaat tattgaagca gctaaactag ataatgcttc tgacttaaaa ataatgttta
     3001 gaataatggt tcctatggca aaaccagcag taataacatt tatattattt aattttatat
     3061 accattggaa taattatttc tggcccttag taatgacaaa tacagatgca ataagaactt
     3121 taccaatagg agttgcgctc cttaaatctt cagaaggaat tactgcatgg aatgtaataa
     3181 tggcaggaaa tataattcta attttaccaa taattcttgt ttatatattt gcgaataaaa
     3241 aagtaaaaga agcttttatg tattcaggaa taaaataaaa aattaaagac taaatttgga
     3301 ggatgaaaat gaagcttaaa agaaaattag caacaggatt agcaataaca ttattattat
     3361 gttctttagt tgggtgttca aattctaatg atgcaaatgc atctaaagga gaaaagactg
     3421 atagcaatat tacgaagata gatttctggg ctcctttagg tggaactaat ggagaaactg
     3481 ctcaaaaaat ggttgaccaa tttaatagtg aacataagga tgtacaagta aatatgttaa
     3541 aacagaaaga ttactatgaa aatgcaacaa aactacaggc agccttaaca tctaaagacc
     3601 aaccagatgt aacattatta gaaataactc aaacaggtac attcgcatca gcaggtgctt
     3661 tagtagatat gagtaaatat tttgataaaa catatcaaga aagattcttt tctggtcttt
     3721 taacaaattc atattatgag gataagtttg ttggtatgcc atttaataga agtacaccaa
     3781 tattatacat aaataaagat atggcaacta aagcaggtct tgacccttca ggtccaaaat
     3841 catgggaaga gcttaaggat tatgctagta aaatgacaaa taaatcagaa gatacttatg
     3901 gttttgaaac accaatagat atttggttct atgaagctat ggtaatgcaa agtggaggag
     3961 aaataactaa tggtaaaaaa gtagcattta ataatgaggc aggacaagca ccagttaagt
     4021 tctggcaaga tatgatgaaa gctggaataa tgaaaatgcc tcctggagaa gattataatg
     4081 cctgggacgt agcaaaacaa gattttgtaa atggtaaagt aggtatgata tttacatcaa
     4141 cagcagattt agcaggactt atgcaacaaa cagaaggtaa gtttgaaata tcaacagcat
     4201 tcttaccaaa aaatcaaaaa tacgcaactc ctacaggtgg agctaatcta gttatgcttg
     4261 aaggtggaac agataaagaa aaagaagcaa gtgcagaatt tatagaatgg atgacacaaa
     4321 cagataaaat cgttcagttt agttcatcaa caggatactt accaacaact gaagatgcaa
     4381 caaactcaga aaagttacaa acattatata aagaaaaacc tcaatataag gttgcaactg
     4441 accaattaca atatgctgtt gcacttccaa tgttaaatgg atacaaggaa gcaactgata
     4501 agctaatgga tgagattaaa aaggggctta ctgatttaaa tgcaaatcca aaagatgtag
     4561 taagcaaagg aacttctgca atgcaagcag taatagataa aacaaataaa taattatata
     4621 gacttgtaag ttgattaaaa gtattcaata atatataaat gaagaggtaa tgtaaatgac
     4681 aaaggttata gtattatcag tagattcttt atttgaaaaa gacttggaat ttgtaaaaaa
     4741 tctaccaaac tttaaatcta tactagaaaa ttgctccata gtaaaaaata taaattgtgt
     4801 ttatcccact ctgacatatc cgtgtcatac tagtatggtt acaggagtat atcctaaaaa
     4861 acatggtata tatcataatg aaaaatttga cccaaataaa gaaaataaag actggtactg
     4921 gtactcaaag gacataaaag caaaaactat tatagatgta gcaaaagaaa ataatctaac
     4981 tacctctagt gtattatggc ctgtaatggg agcaaatcca aatatagatt ataatattgc
     5041 agaaatatgg gctcctagta gagaagatga cccaagagaa acttttgaac gaagttccag
     5101 taaggttata atggacggta tttacaatag acattgtcat tatatcgatt ggaaattaga
     5161 acccaacatg gatttatttg gagtaaactg ttcagtagat ataataaagg aatataaacc
     5221 agatttaatg ctaatacatt tagctacatt ggaccataca cgacacaatt atggcttatt
     5281 taatgacgaa gtagataaag cattaaaaat gaatgataaa tggctaggag atattattca
     5341 ggctacaaag gatgcaggta catacaaaga tacaaatttt ataatacttg gggaccatgg
     5401 tcatcttaga gttgataaag ttataaatcc aaatgtcttg cttaaatcaa atggatttat
     5461 aaaagttgaa aatggagaag ttaaggattt tgatgcctat gtaaattctt ctggaatatc
     5521 agctcaaata atagtaaata acttagatag gttaacagag ttaagagaac tgcttgaaga
     5581 atttaaagaa gaattattca tagagaatat ttttaccaaa gaggaagcat ctaaccttgg
     5641 tcttgaaggt gattttgaaa tggtaattga aggtcttgaa ggaatttcat ttggaaatga
     5701 tttcgaagga agtattataa aagattcaga tataaaagat tacaagtttg ctgttgcgac
     5761 acatggtcat ttacctacta aaggtaatag acctccattt atagcttttg gaccaaatat
     5821 taaaggtggt gtggttatag aagaaggaga cctgagagca catgcagcaa ctatattaaa
     5881 aatgtttaat ttaaaattag atggagttga aaaagaagca tttgatttta ttatgtaagt
     5941 tgaataaagt atttattttc catgaattat agacttctat tgcaaagata gaagtctttt
     6001 tttatgtttt agatttaagt aaaatttaaa tgggtaaaat atttaaaata acttaaacaa
     6061 ttttataaag gtatgatata ataatacatg tcaggaacta atgttcgaaa aaaggagtga
     6121 tactatggat tttaaaataa aatcagactt caaacctaca ggtgaccaac cagaagctat
     6181 taaatcaata gtggattcta taaatagaaa tgagaaattt tcgacattat taggtgtaac
     6241 gggttcagga aaaacattca caatggcaaa tatcattcaa caagttaaga aacctaccct
     6301 aataatggct cataataaaa ctctagcagc acaactttac agtgagttta aagaattttt
     6361 cccagacaat gcagttgaat actttgtaag ttactatgat tattatcaac cagaagcata
     6421 tgtagctcac agtgacaatt atatagaaaa agatgcaagc ataaatgatg aaatagataa
     6481 acttcgacat tctgctacag catctatttt agagagaaga gatactataa taatatcatc
     6541 tgtatcttgt atatatggac ttggtgaccc aaaagactat aaagaactta tgttatctat
     6601 aagaccaggt atgcaaagag acagagatga tgtaataaaa aggttaatag agatacaata
     6661 cgaaagaaat gacataaact ttacacgagg tacctttaga gttagaggag atatactaga
     6721 aatattccca gctagtaatg atgaaaaagc aataagaata gaattttttg gtgatgaggt
     6781 agatagaata acagaaattg attatgtaac agggaagatt gttggtacta gaaatcatgt
     6841 tgttatattc ccagcatctc actatgtaac aacgccagaa agaattgaaa aggcaattgt
     6901 agaaatagaa aacgagcttc aagaacaaat taaatttttc aaggaaaatg ataggctatt
     6961 agaagcacaa agaattgaac aaagaactaa gtatgatata gaaatgttaa aggaaatagg
     7021 attttgtcag gggatagaga actattctag acatattaca ggaaggtcag aaggagaaag
     7081 accatatact ttaatggatt tcttcccaga tgattatttg ataattgtag atgaggcaca
     7141 tgtcactata ccacaagtaa gaggtatgta tgcaggagac aggtctagaa aaacatcact
     7201 tatagagaat ggttttagat taccttctgc acttgacaat agaccattaa attttcaaga
     7261 atttgaagga aatataaatc aaatgttgtt tgtaagtgct acaccagggc cttatgaaat
     7321 tcaacactca gagacaatag cagaacagat aataagacca actggccttt tagacccaat
     7381 tgtagaagta agacctataa ataatcaaat agatgatttg gtaggagaaa taactaaaac
     7441 tatagaaaaa aatgaaagag tgcttataac cactttaact aaaaaaatga gtgaggactt
     7501 gactaactat cttaaagaaa tagggataaa agttaaatat ttacactcag atatagttac
     7561 attagaaaga acagaaataa taagagattt aagattaggg aagtttgatg tccttgtagg
     7621 aataaactta cttagagaag gattggatat accagaagtt tcacttatag caattttaga
     7681 tgcagacaaa gaaggtttct tacgttccga aacagcttta atacagacta ttggacgtgc
     7741 agctagaaat gaaaatggaa gagtaattat gtatgcagac aggataactg actcaatgca
     7801 aaatgctatt gatgaaacaa agagaagaag agatatacaa aatttatata atgaagagca
     7861 caatatcatt cctaaaacta tacagaaaaa tataagagat agtatagaag ctactaaggt
     7921 tgcagaggaa gaagttgtat atggaatatc tgatacagat gataaagacg aaataagagc
     7981 taatatagat aaattaaaat cagagatgat ggaagctgct caaaacttac aatttgaaag
     8041 agcagcagaa cttagagata aagtgaaaca attagaagaa aaattggaaa aatagaaagg
     8101 taaaaacaac attatggaag ataaaattat aataagagga gcaaaagaac ataatttaaa
     8161 aaatatagat ttagaattac caagagataa atttattgtt tttacaggtt tgtcagggtc
     8221 tgggaagtct tctttggcat ttgatacaat atatgcagaa ggacagagaa gatatgtaga
     8281 aagtctatct gcttatgcaa gacaattttt aggtcagatg gaaaagccaa atgtagaata
     8341 tatagagggt ttatcaccag ctatctcaat tgaccagaag actacctcaa aaaacccacg
     8401 ttctacggtt ggaacagtaa cagaaatata tgattatttg agactattat ttgcaagagt
     8461 aggagatgtg tattgcccta catgtggtga aaaaataagt cagatgacta ttcaagaaat
     8521 agttgataaa atgctagatt ttccagatag aacgaaatta caaattcttt caccaattgt
     8581 tagaggacaa aaaggtacac ataaaaaagt ctttgataat atcaaaaaag aaggatttgt
     8641 aagagttaga gttaatggtg aaaactatga agttagtgat gatataatct taaataaaaa
     8701 tcaaaaacat aatatagaag ttgtagtgga cagaatagta attaaagatg gaatagaatc
     8761 aagactagca gattcaattg aaactgcagt taagctatct gatggtcttg taatagccca
     8821 aataatagat ggagaagaaa ttcttttttc aactaaattt gcatgcccag aacatggaat
     8881 tggcatagaa gaattatctc caagaatgtt ttcatttaat gctccctttg gtgcttgtga
     8941 tgtttgtaat ggtcttgggg aaagtaaaga agttgaccca gaattagtag taccaaacaa
     9001 agatttatca ataaaacaag gtgcagtggc agcatggggt tcaacaggtg ttaatgatga
     9061 cacttattac agtaaaatga taaaaagttt agcagagcat tttaatgtat ctttgacaac
     9121 accgtttaaa gatttaccag atgattttgt ggaggaatta ctttatggta aagataatat
     9181 aattgtagag tttacttatg agtcaaaatt tggaggaact agaaattata aatcatactt
     9241 tgaaggtgta atagtaaact tagagagaag atatagagaa actaattcag attacatgag
     9301 agataagatt gaagaataca tggcagagag accatgtcca aagtgtaaag ggatgagact
     9361 taaaaaggaa gttttatctg ttttagttga tggaaaaaat ataatggaag ttacaaactt
     9421 atctgtaaat gaattgattg actttatgga aaatataaat cttacagaaa aacagagatt
     9481 tatagctcat gagattataa aagagataaa aggaagagct atgttcttaa gagatgtagg
     9541 tcttgattat ttaaatttat caagaaaagc tggaacttta tcaggaggag aagcgcaacg
     9601 tataagatta gctactcaaa taggttctgc attggttgga gttttatatg tacttgatga
     9661 gccaagtata gggcttcacc aaagagataa tgataggctt ataactactc ttagacatct
     9721 aacagattta ggaaatacct taatcgttgt agagcatgat gaagacacaa tgagagaagc
     9781 tgattatgtt atagacattg gtccaggtgc aggtatacat ggtggtgaaa tagttgcaca
     9841 gggtacttta gatgaaataa ttgaaaatga gaactcaata acgggtcaat atttaagtgg
     9901 taaaaaacaa atattgcttc ctgagactac tagagaagga aatggaagtt ttatagaaat
     9961 agttaaagca tcagaaaata atctgaaaaa tattgatgta aagttcccat taggtaagtt
    10021 tacatgcata acaggtgttt caggttctgg aaaaagtaca ttaataaacg atatacttta
    10081 caaaggtgtt gcaagtaaag tgaatagact taagcaaaga gctggtaagc ataaggagat
    10141 aaaaggaatt gaaagtatag ataaagttat aaatatagac caaagtccta ttggaagaac
    10201 acctcgttca aatcctgcta cttatacagg tgtatttgat tttataagag atatttttgc
    10261 atctactaat gaagctaaag caagaggtta taaaaaaggt agatttagtt ttaatgtaaa
    10321 aggtggtaga tgtgaagctt gtaagggtga tggaataata aaaatagaaa tgcatttctt
    10381 accagatgta tatgttcctt gtgaaatttg taaaggtgaa agatacaata gagaaacatt
    10441 gcaagtaaaa tacaaagata aaactatatc tgatatatta gatatgaatg tggaagaagc
    10501 tgttgagttt ttcgaaaata taccaaatat aaaaagaaaa ttagaaactt taatggatgt
    10561 aggactttca tatattaaat taggacagcc ttcaactcaa ttatcaggag gagaagcaca
    10621 acgtataaaa ttagctgctg aacttagtaa aagaccaaca ggaaagactt tgtatatatt
    10681 agatgaacct actactggac ttcatatggc agatgtagac aagctaatcc aagtattaca
    10741 aagactagca gatacaggaa atacaattat agtcatagaa cataatttag atgttataaa
    10801 gacttgtgat tatgtaatag acttaggacc agaaggcgga gataagggtg gaactatagt
    10861 tgcaactgga acaccaaaag aagtatctaa agttgatggt tcttatacag gtcaattctt
    10921 aaagaaatat ttttcttaag ttatataaat tgtgaaaaat tttagagcag atatacgata
    10981 aactgttttg ttataagtaa attagtcaaa tataagttgt aaatagtaat acaaagtaaa
    11041 gtccatatta ctcttcatgg ttaacattgt tttcaaatca attatagaag ggaatatggg
    11101 ctttaacttg tttaaagcac tggaaattgt ataacttttt aaaattcata ttttagggta
    11161 taattttagt ataaaattta atgttttgga gggcgagatg tttgatatac aagagcattt
    11221 aaaaaaatta ccttctgagc ccggagtata tttaatgaaa gacaaatacg accatataat
    11281 atatgtggga aaagccatat ctctaaaaaa tagggtaagg cagtattttc aatcttcaaa
    11341 aaaccataca tctaaagtaa aatctatggt taagaatata tataagtttg agtatattat
    11401 aacggattca gagttagagg cattaatact tgaatgtaat ttaataaaaa gatacagacc
    11461 aaagtataat gtagtgctta gagatgataa aacttatcca tatataaaag taactacgaa
    11521 tgaagattat ccaagaatct taaaagttag aagagttctt aaagataagg caaagtattt
    11581 tgggccctat accaatataa ctgctgtgaa tgataccttg gagcttataa gtagtacgta
    11641 tccaataaga agctgtaaaa tagatataga taaagcaata aagaataaga caagaccttg
    11701 tttaaatcta cacataaata aatgtttagg accttgtaca ggaaatgttt ctaaagaaga
    11761 gtatggaaaa atgatagaag agattataat gtgtttatct ggtaaagaag aaaaattgat
    11821 ggaactttta aaggaaaaaa tgaatgagag ttctatgaac tttagatttg aagaggctgc
    11881 tgtatatagg gataagataa agagccttga agagatgata cagaaacaaa aaatagatgc
    11941 tactgtaagt gacttaaacc aagatgtggt tgcaatggca agagctcata atgaagcttg
    12001 tgtccaggta ttttttataa gaaatggtaa gattgtaggt agggaacatt ttatattaga
    12061 aggggtaatg gatagtccaa gagcttctat attaagttca tttgtaaaac aattttataa
    12121 tgaacaagag tatataccta aagaattaat tatagaagat gagatagaag atagttctat
    12181 attagaagaa tggctgtcat ctaaaaaggg gcaaaaagta acaataagag ttcctcaaaa
    12241 gggagagaag aaaagcttag ttgaaatggt taggaaaaat gctgtagaat atcttgaaaa
    12301 attttctgat atgaataaaa gaaaatatga aaaaagtatt ggagcattgg aagaattaaa
    12361 gcaaatatta aatctagaaa agttgccaat aagaatagag gcttatgata tatcaaatat
    12421 acaaggtgta gattcgattg gttctatggt agtgtatacg aatgctaaaa aagataagaa
    12481 ggaatatagg agatataaaa taaagacagt tattggacct aatgattatg attctatggc
    12541 tgaaattgta gatagaagat taaaacatgg aaatttacct gacttgatat tgttggatgg
    12601 tggaaaagga caagttagtg cagtaaaaaa agtactagaa ctcaatgatg tagatatacc
    12661 tctttgggga atgtacaaag atgataagca tagaactaaa ggattgatat gcaaagaaaa
    12721 agaaatagaa ctagataaaa caactaattt atatagattt attgctagta tacaagaaga
    12781 agttcataat tatgcaataa catatcatag aagtctaaga aataaagctt tgacaaaatc
    12841 tatcttagat gatatacaag gaataggtga aaaaagaaag aaatcattat taaatcactt
    12901 taaagatgta gatgctataa aaaaagctac tatggaagaa cttttagaag tagatggaat
    12961 gaataaaagc attgcagata atgtttataa tttttttaga aaggaagaaa attaatggaa
    13021 actagcaata aagtatcaat aagagagata ataaaagagt tgaagttaaa agtagtatac
    13081 atgcctgatg atgtagatta ttatgtctac tctcaagata taaatagacc aggattacag
    13141 tttgcaggat attttgatta ttttgcatat gaaagaatac aaatagttgg aaagacagaa
    13201 tataattttt ttggattaat ggatagtcat attcgggaag aagtactaga tagatttttc
    13261 tcatatgaaa taccagcgtt aataatttct agagacttgg aaatcaagcc agatgtaatt
    13321 gaaaaggcta aaaaatataa aagaatactt ttgagcacaa atagaaatac cactagactt
    13381 ataaatagac tgtcaaacta tttggataat aagttagcac cacatacaac aattcacggt
    13441 gtgctagtag atatttatgg tataggtgta ctgataaaag gtgaaagtag tataggaaag
    13501 tcggaaacag cattagaact gattcaaagg ggtaatagat tagttgcaga tgatgctgta
    13561 gaaataagaa aattagatga aagtatgctt gtgggtcagt cacctgaact aataagacat
    13621 tttcttgaga taagaggaat aggaataata gatgttagaa gtttatatgg agtaggggca
    13681 atcaaaaatt ctaaaatgat agatttagta gttcatttag aagcttggga tgaaactaag
    13741 tattatgata gactaggact ggataaagaa tatgaagaaa tacttggtgt taatgtagag
    13801 aaattagttg tacctgttaa acctggaaga aatactgcta tgatattaga agtagcagca
    13861 atgaatttta gacaaagagg aatgggatac gatgcagctc aagaatttac taaaaaatta
    13921 tcaaaattaa tcgatactaa gtagtactga ataatagtgg actacacatc atttaattaa
    13981 atattgatta ggattatgtc ctacacctta taaattaaaa tcaagttgta tatataatgt
    14041 ttattaataa gatatattat ataaggataa tttaaagtac agtgttagat tataataata
    14101 ctagattaat tttatatttg agtttcatta tataacaaga aagttggaat tttttatggg
    14161 aatagaatat tttagtaaac ttttagaaag tagtactgaa aaagaattgc tcttaagtaa
    14221 aaagtcaaat aacataagta cagcaagatt aatatctttt ttgatagtga tagcagggct
    14281 tgctattgga ttttataata aaaatatagt aggtattttt gttggagttt tatcaataat
    14341 aatatttata gcactcttag ttattcatag taaagtaaaa gaagaagaaa catattttaa
    14401 aagtaagagc gaagtattaa ataagtatat taagagattt ggagatggtt ggaaggaatt
    14461 taaaatagat ggtaaagaat acttaaaaga tgaaaattcg caagcaaaag atttagactt
    14521 atttggaaga gcatcattgt atcaatatat ctgtgtagct aatacatctt atgggaaaaa
    14581 ttttttagcc aaatatttgt ggaatgaaaa tccagatgag aatataattt tagaaagaca
    14641 gagagctata aaagaacttt tatcaaaaga tgatttttct attcatattc aaacactcag
    14701 taatataatt ggtaaagagc aaaaaaacaa ttcagatagt agtatagaat cgtttataga
    14761 atatggagaa aataagaatg tgtatatacc aaaatggatg catattttta cttggggttt
    14821 accaactgca acaatcttat cttttatatt ttgtatgtta ggatttttac ctgttttacc
    14881 tgttttttta ctttttataa tccagttagg gtttagtggt tttggatatc caagacttat
    14941 gcaaactctt gctccattat tttcatttag tagaagtata caagtatatg aaaaaatgtt
    15001 tgaggtttta gaaaaagaga cgtttgagag tgcatatttg aaagaattac aagataaact
    15061 ttctaaggga agtggagttt caagaggtgt taaacaatta aattcaatag gaaatgctgt
    15121 aaacctaagg tataatcaga tactatatat aattgcctgt ggtgttttga tgtggaacta
    15181 tcattgtgca gaagctttgg agcgatggaa aggtatatat ggaaatcaaa ttagagattg
    15241 gtttgaatct attggagaat ttgaagctct aataagttta acagttgttt ctcatgtaaa
    15301 ggaaaataca tgttttccag taataaaata tgaagatact ccaagattga aagttgaaga
    15361 ggtataccac ccattaatag cagaaaaaag tgtcatagca aattcgatac aattaaattc
    15421 tcagacttgt attatcacag gttcaaatat gtctggaaag acaacatttt taagaagtat
    15481 cggtgtaaac ttagtgctgg catatgcagg tgctccagta tgcgctaaga gttttgatgc
    15541 gacctgtatg gctatattta cttctatgag aatacaagat gatgtaagtc aaggaatatc
    15601 aactttctat gctgagattc ttagaataaa atcaatgatt caatatagtt taaaagaact
    15661 gccaatgttg gttctagtag atgaaatttt taagggaact aactctgctg atagaattat
    15721 tggagcaagt gaggcagtaa agaaattatc aaaaccctgg gttataagta tggtgacaac
    15781 acatgacttt gagctatgtg acctttcagg tagcggagat gttgaagttg taaactatca
    15841 tttctcagag tactatgtag acgataaaat tcattttgac tataagataa aagatggaag
    15901 atgtaaaact acaaatgcaa aacaacttat gagaatggca ggtattcttt aattaataga
    15961 taggttaatt aataaataag tctatttaga aatataattt aatttgtgaa attaataagg
    16021 attgtcttaa attacgtaaa agtattttaa gacaatcctt ttttaagaat aaattttata
    16081 ctttgttatg actacttagc ttcttcacaa gcacattctt gtggacactc tccatagaca
    16141 caactcttat caaagtaatg agtatgaagg tatttatgag ctacatcact atttggttct
    16201 ccaaagaagt ctttataaat agcttgtaaa tcaggattct catgagattt acgaatagcc
    16261 ttatttctat ctatttcata taacgcattt gcacgtctat caactatttc agtattacca
    16321 tggtgataag gttgaccagc acctccaaca catccaccag gacatgccat aacttctatg
    16381 atatggtatt tacattctcc acttctgacc ttatccataa tctttctagc gtttttaagt
    16441 gtatttacaa tacatacatt aactgtagtt ccatcaacat ctatacttgc ttccctaatt
    16501 cctttaagac ctctaacatt agtgaagttt acattatcta aagtcttatt agttatcttc
    16561 tcatatgaag tacgtagggc agcttctaaa actccaccac ttgctccaaa tatagatgca
    16621 gcacctgtag attttcctag aggattatca aaatcttggt cttgaagatt aggtaaatcg
    16681 attgcagctt cttttatcat cttagctaac tctctagtag ttatagaaag gtcaacatct
    16741 agtattccag attcacttaa ttcttctctt gaagcttcat attttttagc tacacaaggc
    16801 attacagata caacataaag ttcttcagga ttgattccta atattttagg tgcatagtag
    16861 tttttagcta tagaaccaaa cataccttga ggagacttac agctagatgc taagtttaac
    16921 ttgtctggat aattgtgctc taagaagttt acccatgcag gacaacaact tgttaatata
    16981 ggaagatttt cacctttttg gattctttct ataaattcag tagcttcttc cattatagta
    17041 aaatctgcac caaagtttgt atcaaataca tgttcaaatc caagtgcttt taaagcagct
    17101 accattttcc ctgtagagat tgaccctggt tctaatccaa actcttcacc aatagcaact
    17161 cttacagcag gagcaacttg cactactata gtcttttctt ttttatttaa aacatcccaa
    17221 agtttaggta cattatctac ttctgttaat gctccagttg gacagacaga tatacattga
    17281 ccacagaatg tacactctgt ttcaaccata tcagagttaa agaatgtact aactaaagta
    17341 ttaaatccac gattaactcc agataatgct cctactgttt gtatgtcatt acatacagtc
    17401 tcacatcttc tacataatat acacttagaa tggtctttaa ctaaagattt agtagaagta
    17461 tctttacctg caaaagattt agaaccttga tatcttattt ttcttactcc taaatcagca
    17521 gctatagtct gtaattcaca atctccattt ttttcacaga caaaacaatc ttgtggatgg
    17581 tctgataaca ataattcaac aatagttcta cgagcattta atgctttagc actattagtc
    17641 tgaactttca taccttcctt tataactgtt ccacaagctg gaactaatcc tctctctgtc
    17701 tcaaccatac aaacacgaca agaagcacaa gtgtcaagct tatctatttc attcatatgt
    17761 aaatgacaaa ggttgtgtat ttttatattt attaatttcg cagcatctaa gatagaagtt
    17821 ccactaggtg ctgaaacgtg tttgccatta atagttaaat taactaaact cattgttatt
    17881 cctcctccaa ataagttatt tcttgattat agcgttaaat gtacaattat ctatacaagc
    17941 accacatttt agacacttag aagtatctat tgtatgttgt tccttaacag aacctgttat
    18001 acaaccagca ggacacactc tagaacattt tgtacaaccc ttacagtcat ctgttataaa
    18061 gtaagaaagt aagttctgac atttaccagc aggacatttc ttatctacaa catgagctat
    18121 atactcatct ttgaaatatt gaagagtact taaaacaggg tttggagcag ctttaccaag
    18181 accacataat gaagcagttt gtatagtttc agcaaggttt tctaggtcat ctaaatcttg
    18241 tagagtacca ttaccttctg taattttagt taatatttct aataatcttg tagtacctat
    18301 acgacaaggt gtacatttac cacaagattc ttcaacagta aactcaagga agaacttggc
    18361 tatatctacc atacagtcag attcatcaag aacaagcata ccaccagagc ccatcattga
    18421 accgatagaa cttaatgaac caaagtctat aggagtgtct aaatgttcag ttggtataca
    18481 tccaccagat ggtccacctg tttgaacagc cttaaatttc ttaccttcag ggattccccc
    18541 accaatttca aatacaatat ctcttaaagt agtacccatt ggaacttcaa caagtcctac
    18601 attttctacc tttccaccaa gagcaaagac tttagtacca ctagaatctt cagtacctaa
    18661 gttggcaaac caatctccac ccttaagtat aattgcaggt atgttagcaa aagtctcaac
    18721 gttatttaag caagtaggag atttccatag accactctta gaagaactat aagttttcat
    18781 tctaggttca ccacgtctac cttcaattga atgcattagc gctgtacctt caccacaaac
    18841 gaaagcacct gcaccatatt ttaattctaa tttaaagtta aatccagtgc ctaagatatt
    18901 ttcgccaagt aaaccgtatt tttcagcttg agctatggct acttttaatc tttctataga
    18961 ttttggatat tctgctctta tgtatatgta tcctgtatca gaacctatag cgtatccaca
    19021 tattgccata gcttctagta cactatgagg gtcaccttca agtacagatc tatccataaa
    19081 tgcacctggg tcaccttcat cagcattaca tacaacaaac tttttatctg ttggaccagc
    19141 tggatttttt gaagcagctt cccatttaga gccagtaggg aaacctgcac cacctctacc
    19201 acgaaggcca gaagttttta cctctgcaat aacttcttga ggagtaagag aagttaaaca
    19261 tttgcctaga gctaaataac catcattggc aatatattct tctatactat ctgggtctat
    19321 aagaccacaa ttttttaaag ctatacgtaa ctgttttttg tagaaagaca tttcttcttg
    19381 ttcttcaact tttttactta aagattcttc ctcatataat aattcctcga caactgtatt
    19441 tctaattaag tggctttgaa ctatcttttc agcatcagat ggctcaactt ttacgtagaa
    19501 aacgttatca ggataaactt ttacaatagg accttgagca cagaaaccaa aacaacctgt
    19561 caaacgaaca ctaactttat cttggattcc tgatttcttt atttcatttt ctaattcact
    19621 aactatttct agactgtttg aagatgtaca tccagtatca caacaaacaa gtaattctct
    19681 tcttaataca ttttccttag aataattttt tcttaagcta agattaggtt tcagctcatc
    19741 agctaaaact tttaattcat caaatgaatt tactttacgc attatttatt cacccctctt
    19801 aacaattcaa ttctaattct ctataagtat ctaatatttc gcttacttgg tcaggtttca
    19861 ctttaccata aactttacca tttacagtaa caactggagc taaaccacat gcccccaaac
    19921 atcttaaacc ttctaaagaa aatttaaaat cactagtagt ttctccaact tttatgccaa
    19981 gctgtttttc aaaagcactt aagattttgt cagcaccttt tacaaaacat acagttccta
    20041 gacagacact gattttatac ttacctacag gttctgtagt aaaataagaa tagaatgtaa
    20101 caaccccata tacctttgca ggagctactc caagttttct agcaacatga agttgaactt
    20161 cttttggaag atatccaaat ataccttggg cttcatgtag aacttgtatt aacgcacctt
    20221 cctttgttgc aagagagtca ataaatatat ctagttcgtc aaataattgc ttgttgtgtg
    20281 aaataaaatc gcacataaca aatccccctt ttctttttaa agttattatt tttcataaaa
    20341 taattaacca tgagttgacc ccaacattat gtgggtgaac aaaaccaaaa caataaagaa
    20401 agaataatat acaacaattt ctttatttat aaaaacaatg caaaaaagtt acctattaaa
    20461 aataaaaact atactagtct attataatat aaaataattc agaatagtta aaaaaaataa
    20521 aaaaaatgag ctaaattcaa taaaaaaaaa ataatacata taaaaaactt taaaaaataa
    20581 accaaaatgt aaaattttta taaaataaaa gatttataaa actgctgata atagtcttga
    20641 aattgattcc ttgattttta tataagtaga cctagaatta tatatatcta atgttatttc
    20701 cttagaattt aaaatatcat tttcaaatat aattctttgt tccaatgctt tttttgaaga
    20761 gtacataaat gcattaactt cgaaattaag ctcaaaactt ctgatatcca tattagctgt
    20821 tcctatagag catatagagt catctataac tattgtctta gcatgtaaaa atgcattttg
    20881 accataagta tagattttag ctccaaactt taaaagttct ccagcatatg aatatgatgc
    20941 ccagtataca aagggatggt caggtttaga aggaatcatc actctcacat caacaccaga
    21001 taaacaggct atttttaaag tatctataaa agttgagtca agtattaaat aaggactctg
    21061 tatgtatata tactttctag ctttctgaat cattttaagg taaccatatt taatctcatc
    21121 aagttctgat atatctggac cacttgatac tatctgaatc ccaatatttt cagaaggatg
    21181 attagaattt gtatcttctt ctataaagta tttttctaaa tctaagtctt ctttagttgt
    21241 atatctccaa tcaaggatga atctcatatt taggtcaatt acgcaatcac cagtaagctt
    21301 agtatgtgta tctctccata gaccaaattt tggatttcta cctaaatatt catcaccaac
    21361 attatatcca cctacaaatc ctaccttacc atcaatgaca acaatttttc ggtgatttct
    21421 atagtttaag ttaaagttta caattttcaa gaatgatggg aagaagcttc caactttaac
    21481 accactttct ttaagtctgg aaagtgtact attttttagt gttctaccac caactgcatc
    21541 aaataaaagt cttacttcaa caccttcttt agctttgtct ataagaatgt ctattatttc
    21601 agttccgatt ttgtcatctt tgaaaatgta aaattgtatg tttatatatt ttttagcttt
    21661 tttaagttct tcaagaagac tgttaaaaaa ttgactagat tcagcataaa tccacacatc
    21721 gttattattg gtataatgag cattatttga attagccaat gcataaatca tatccttatg
    21781 ttgatgtata tctgaatcaa tttcagatgt agattggagt ttaacctgag tatctagtat
    21841 attacttttg attatcttat catccttttc tttcaatcta aacatattgt tttttgaaat
    21901 atttctgcca aaagctatat aaagtatgaa tccaagagca ggaatcaata ctaatataag
    21961 aagccatgtt aaagttgtat ttatgtttct tttttctcta aagataaggt ttagtataac
    22021 catgaaatta attatatata ttgtagtaac tgctatttca tatattgaca tttcgaaaaa
    22081 tgatatatca aacataaaat tccctccttc aaatatttat taaaataagt aattatatat
    22141 aataacacaa acatatatct attatatata atacctcaat aattaaaaag taaaataaat
    22201 aatcaaaagg taaat
//
LOCUS       DAMJZE010000046        22111 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJZE010000046 DAMJZE010000000
VERSION     DAMJZE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.046"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(217..1323)
                     /locus_tag="PC541_001594"
     CDS             complement(217..1323)
                     /locus_tag="PC541_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2839061.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(1358..2899)
                     /locus_tag="PC541_001595"
     CDS             complement(1358..2899)
                     /locus_tag="PC541_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2839062.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(3261..4007)
                     /locus_tag="PC541_001596"
     CDS             complement(3261..4007)
                     /locus_tag="PC541_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2839063.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(4219..5529)
                     /locus_tag="PC541_001597"
     CDS             complement(4219..5529)
                     /locus_tag="PC541_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839064.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(5542..6012)
                     /locus_tag="PC541_001598"
     CDS             complement(5542..6012)
                     /locus_tag="PC541_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839065.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(6016..6867)
                     /locus_tag="PC541_001599"
     CDS             complement(6016..6867)
                     /locus_tag="PC541_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2839066.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            7791..8702
                     /locus_tag="PC541_001600"
     CDS             7791..8702
                     /locus_tag="PC541_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2839067.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            8877..9374
                     /locus_tag="PC541_001601"
     CDS             8877..9374
                     /locus_tag="PC541_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2839068.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            10118..10480
                     /locus_tag="PC541_001602"
     CDS             10118..10480
                     /locus_tag="PC541_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2839069.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            10545..11162
                     /locus_tag="PC541_001603"
     CDS             10545..11162
                     /locus_tag="PC541_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2839070.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            11159..11644
                     /locus_tag="PC541_001604"
     CDS             11159..11644
                     /locus_tag="PC541_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2839071.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            11687..12205
                     /locus_tag="PC541_001605"
     CDS             11687..12205
                     /locus_tag="PC541_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2839072.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            12341..13300
                     /locus_tag="PC541_001606"
     CDS             12341..13300
                     /locus_tag="PC541_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2839073.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            13326..15029
                     /locus_tag="PC541_001607"
     CDS             13326..15029
                     /locus_tag="PC541_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2839074.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            15290..17392
                     /locus_tag="PC541_001608"
     CDS             15290..17392
                     /locus_tag="PC541_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2839075.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            17405..17845
                     /locus_tag="PC541_001609"
     CDS             17405..17845
                     /locus_tag="PC541_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2839076.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            17881..18684
                     /locus_tag="PC541_001610"
     CDS             17881..18684
                     /locus_tag="PC541_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2839077.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            18724..19320
                     /locus_tag="PC541_001611"
     CDS             18724..19320
                     /locus_tag="PC541_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2839078.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            19426..20289
                     /locus_tag="PC541_001612"
     CDS             19426..20289
                     /locus_tag="PC541_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2839079.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            20356..21345
                     /locus_tag="PC541_001613"
     CDS             20356..21345
                     /locus_tag="PC541_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2839080.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            21437..22048
                     /locus_tag="PC541_001614"
     CDS             21437..22048
                     /locus_tag="PC541_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2839081.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         8591 a         2606 c         3717 g         7197 t
ORIGIN      
        1 gctagaataa attattatgc aaaatattga agcttattat gaaaaatgtt agaacaaatt
       61 actatataaa atatcaaagc ttattatgaa aaatgttaaa acaaattatt atataaaaat
      121 actaaaacct atgtaaaaca actctaatat ataacttata aagttgagga tacataggtt
      181 tttattttta taatttaaag agttctatta tgatatttat aatttaatca tatgataaac
      241 tattttggca gtatcatata attcgtctat atttacatat tcattaactg tgtgtacatc
      301 atacatacct agacttaata tagcacaatt atatccaaga ttagctaata tatttgcgtc
      361 acttccacca ccaattacca ttggatttgg aacaacacca acttgtctta ttgcttcttc
      421 acttaacttg aaaacatgac tatctctact aagttcaaaa ctagggtaag acatattatg
      481 ttcaaaagta taagtagtat taaatttaga tgcagcgtcc ttacaacatt tttccatatg
      541 attaagctca tactctaaag tttcaggtat atgagaccta atttcagcag tcaatgtaac
      601 cttatcagtt actatgtttg tagctccacc accttctatt tcacctatat ttgaagtagt
      661 caatgaatct attctgccta tgtgcatgtt gcttattgca tgagaggcta ctaatatagc
      721 atttaaccct ttttcaggtt ctataccagc atgtgctttc tttccttgaa aagagatttt
      781 tatagattgt tgagcaggag ctttgtatgc tatcgaacca atagctccac cagaatctaa
      841 aatcaccata tctttacaag gcaagttgtc aggattaaag tttttgacac catgcatacc
      901 agcttcctca cagattgtaa aacaaagata aatatctcta tgtggtattt tttctgtaat
      961 aacatgttct aaagcttcaa gtatagcagc tattccagct ttatcatctg caccaagtgt
     1021 agtagttcca tcacttctaa ctacattacc atctagtatt ggttttacac ctaaacaagg
     1081 ttgaacttgg tccatgtgtg cacataaaca aagtggtcta cttccttctt caccttttat
     1141 ataggcaatg acattacctg tattaccacc atatttttca cctgcatcat ctataatggc
     1201 atctatattt ctttctttta agtagtttac caaccactta gacatttcta attcttgatt
     1261 agatgggcta tcaattctaa ccatatccat aaaattattt ataagtctat ttttatctaa
     1321 cataaattat ttctcctttt agaaagattt aaataaatta catgctatat gaaggaagta
     1381 ttccaggtcc tagtggaata ttaaatacca taaacaatat taatagtata gtccacacta
     1441 ttaagaatga tatagagtat ggtatcatat tagcaattac agtacccata ccagcatctt
     1501 tatcgtattt tcttgtgaat gctagtataa tagggaagta cggaaataat gggctaagtg
     1561 gatttgttat acaatcacct atacgataag ccatctgagt aagtgctgga ttgtatccaa
     1621 ccatcataaa cattggtaca aatataggtg caagaatagc ccactttgca gatgcactac
     1681 ctataaataa atttataaaa catgataata taataaatcc tatcaacaat aaaggtccat
     1741 ttattcctgc tgattttaaa aattctccgc cctttacagc tagtatcaga cctaaattac
     1801 tatttgtgaa taattgcaaa aattgtgaag cagcaaatgc tagtattata tatccaccca
     1861 tatcactcat tgcactagcc attatagaga ctacatcctt atccttcttt attttaccta
     1921 tagtttttcc gtatacaaca ccaggtatga aaaatgctag tgctattata ggtaccatac
     1981 ctttcataag aggagcattg tttgatagta aatctcctgt ttctgggtca gctaagaatg
     2041 gttttttacc gattacacta agagctacta taatagccac atatattaat atacttatac
     2101 tagctttttt aagccctttc ttttctacat cactaagact tccatcaaca tctagttctg
     2161 catttccttc atatttacca aatctaggag ctacaatttt ttctgttacc catgttgcca
     2221 aaagtattag aacaaaagtt gatgctatca taaaaaataa gttcattgtt gcattggctt
     2281 gaaatccaga atcaatcatt tgagctgctg gaactgtaaa acttgcagca agtatatcat
     2341 tgacacttat cataatgttt gcggcaaatc ctagacagac tgctgcatat ccagaaaata
     2401 aacctataag tggatgtcta ccaacaccaa gatatacaag tgcagctaat ggtggaaata
     2461 atattgtacc tgcatctgaa gctatattgg caagcatacc tatagtcatt acaacaagag
     2521 ttactcttgt ttcaggaatc ttgcttactg atgctttgat tgttgctgtc ataagtcctg
     2581 ttttgtcaca tacaccagca cctatcatag ttactagaac tagacccaaa ggagcaaacc
     2641 cttggaaatt gctgactatg tttcctaaaa gaatttgaag ctgttctaca gtcaataaat
     2701 ttacagcggt tatagtttct ccagtaccag gatgaactac tgatataccc atagaaccaa
     2761 ctagactaga tagtataagt aaaagtacac ataaccctaa aaatattgta actgggtctg
     2821 gtagtttatt accaacagtt tctattctgt ttaatgtcct gtcaaaaaag tttttcttct
     2881 taactacgtt atcattcata atatcctccc taatgtttaa aaatataatt aaaaaataag
     2941 tattgtggtt aatcttacaa tttaaattct gtttgtcgat ttaagacgtg tccacaatat
     3001 gtcctaaaaa aagtataaaa taaacttttt cctgtgtcaa tgcagttttc tgaattttct
     3061 ggttgagtgg tgttagttaa atatgcttta agtagattaa tgacgaattt tatctaatgt
     3121 atttaaataa catatgcact ttaaaattaa ttttaaaaca aaattttatg aaaagctagt
     3181 ggaagattaa gttattaaaa taagtagcgt agtaaagtgt tagatttttt aattaatcaa
     3241 ataaatttac tttgtatttt ctacaaaaat ctgtgaatat cttttgaatt atgtagagga
     3301 tataaattta ttttatttac atagattaga ttttgttcta atttactgta ttcaaatggt
     3361 tttccatgtc caggataaat cattttaggt tttgtattga tgattgtttt ccaagattga
     3421 ataaattcta gtttgctttc tgcaaatatg gtaattttgt gagtacttgg aaatccattc
     3481 attgcagaat ctccacaaaa taaaacacct tcatcaatta gtagtgaaat tgaatcatca
     3541 gtatgtcctg gtgtatctat aatttttcca tttagttctt tttcaatttc tttacgattc
     3601 ttgtcagaca caagaataca ccttttttca aattcttgtt taagtggaga aaaactatgc
     3661 tcaccttttc caaatagttt cataatttgg caaaagagaa aggcagtacg gctggtacaa
     3721 ccaccaataa aagagttttg acctttatat agaatttcta aagctttatc atgcataaca
     3781 atctttgtat taggagattc gtctagaatc tgatttagaa aaccaacatg gtcatcatga
     3841 gcatgagtta gaaatatgta tcgaatatct ttgattttta tgttgaattt agaaattcgt
     3901 tttttgaaat gtttaaaacc attttcatat ccagtatcaa tcaagacaaa gccatcttga
     3961 atagggtaca accatgtatt tataattcga tttcctgcat taaacatttt ttcaccacct
     4021 tgaattagaa tagaaattta tattaattag aaagaatata gcatagttct actgatattt
     4081 caacgtagct ctaaaagttt taaggaggat aatatatttt agtttaatct aaaaactaaa
     4141 agaaaagaag tatactgtta caaaaacaaa gagtggtata acttttacag tagataccac
     4201 cctattataa aaatagactt ataaagtttt atcgtgtttt tctaagaatg ttagatattc
     4261 ttctttagtc ataactggag taaaggtatc taatatatct ttagcatcct tagcattatc
     4321 aaaaagaagg tctacaactg taagtgccat tgctttagca ggaattatgt aagctaaatc
     4381 ttcatcaact atttcaaaat ctcttgtatg aagagctcct tttacaccac caatcatagg
     4441 gtgaagagta ggcattatat gagacacatc tccaaaatca aaagagcctg taaaatctcc
     4501 accatcaata acttttcctt ttagacctaa atcttcaaga ttgtttttaa ataaattatc
     4561 aagacttttg tatcgtaata ttggaagata tccaggaatt tcagttatct caacatcagc
     4621 tccaactgcc attccaccag ccatcaaagc cttatttatt ctttcattag catcaatcat
     4681 accatttata gttctagctc taacatatga ttccatatga acatcagcag gaacaacatt
     4741 tactatatct ccaccttttg tgatgatagg gtggaatcta actctttcag actctttaaa
     4801 agtttctcta agtgcattca cgttgtttat agcaagcatt gctgcattta aagcatttat
     4861 tccatcatgt ggagcagaac cagcatgaga ttctttacct ataaatttaa ccttttttcc
     4921 aacaaatcca ttactctcag gaccaactag agccttatct tctcctaaat cagaagcatg
     4981 aaacatcata gatatgtcta tatcatcaaa ataacctctt tttattaatt cttgttttcc
     5041 accaaaataa gttatttttc cttctttttt taattgctct ctgtattcca actcaataaa
     5101 ctcttcagca ggagtagcca taaaagatac tttaccatct aaatgttcta aaacgccact
     5161 ttttactaaa ccaacagcag cacctaacat tccagctatt tgaatattat gtccacaagt
     5221 atgagaagca cctatttcat tagcatcttt atgttcttta catgatatac catccaattc
     5281 tccaagtata gatatatgag gacctttctt gctagaattt gcatctgctt tgcatcctgt
     5341 aactgctata tttttttcta cagataaatt taattcttgc tcaaaaaagt ttgaaacagc
     5401 ttctgtagtt ttaaattctt tatatccata ttctggattt ttgtatattc cttgacctat
     5461 ttttaatatt ttttctctat tttcatctat agtttttatt actagagatt ttaattgttc
     5521 tttattcata ataaattcct cctatatttt accttgaata cttaaaacta tatgagctat
     5581 actcgcacat gcaaagaata tagttgtaaa tacaataagt gaaattataa ctattttcca
     5641 tgacatttct tttaaagctt ctattttgtt tccaacagaa attcctgcaa aagctagtag
     5701 tggagtagtt atagccataa agttaatatt attagtgtta gttaagaata cttcagatgt
     5761 tggactatat ggcatactaa gtaaaaggcc gattatagtt gcaaatccaa aagctggaaa
     5821 gattgattta ggaaataaat cttttataat catagctgcc atagcagcta atattagcat
     5881 caccatacca ggaagagctg taactataga tgtaccaacg ctaattcttt gtcctataag
     5941 aattataact ccaataacag acataaccaa taattgatat gaacttttct ttaaatttaa
     6001 attagcaaac atttattagc actctccttc tgtagattta atttctttag tttttttatt
     6061 tctcgaaaat ttaggttcta aaatcttata cataaaatta gtaaatggta aggatacaaa
     6121 aactaagaat ataattcctg ttatactaga catcatattg cttgttgcag cataggcaag
     6181 tattgtatct gccatttcag gtggtaccat ggtagataat gaaccagcag ctgctgtcat
     6241 catacttcca ctaccaacac cacaggccat tcccaaagca tatgggtgaa gaccactata
     6301 aatagatatt gaacctagta cactaaagta tatagtacca agaactgttc ccattaaata
     6361 tgtaccaagt acaccactac cttctggtga gtttatccca tatttttctg atataactcc
     6421 taaagaaggt tcacgactta tacttgcagt agctccaaca gcttctcttt tcattccaaa
     6481 cattaatgca agaggtaatg caacgattgg tgcaagtata tgaccaaatt cttgagcgat
     6541 aaacgctggt ccagcttgaa ttattttgtc aatgtttggt ccaactgtag taccatattt
     6601 tataccaagc attactagtg caattccaac aacttcacca gcaatattta tttctttttc
     6661 tccaaccact tttttaagtg ctttaatttt tttacctaat aaatctggtg ttattataag
     6721 tccaattata actgcataaa gcatagggaa cagtactata gaggcttttc caatagctat
     6781 agttctttgc cctataaatt cacatattac taccaagatt aagcaagtta aaaatacttg
     6841 aataaaatgt tttttattaa tttccatgat ttcctcccca aaagaataaa tattttactt
     6901 atctgaaatg agaaaatacg ttttcttaag tataatacgt tttcctaaat aaaatgaaaa
     6961 aactatttca aataagtttc agaaatgtca ataacatgtc cttaattaag gataaaacaa
     7021 aaatttaaca ttgtcaacga aaaatatcta aaatattaca tattttgaat atattgtaat
     7081 atagctttat tatgaaaaaa ttgtttaaat attgtaggaa taatagctaa aaagctttaa
     7141 aattagtaat ataaaattga aaaataataa tatagagttg agaaatagta aattgaaatt
     7201 ttaaaataaa tgagtggaag tagcttagta aagtatggaa atgaatatta caatagaaaa
     7261 taaataatat gtaaagactt taaattaatt ttaagtatta agtacaaata ctaattttga
     7321 tatagaaatt gaataaagta aatgataata agcaaatgaa ttaagatgat aataagtgaa
     7381 ttgatataga aataagtaaa ttaagtaaga cgataacaag tgagataata ataaatgaat
     7441 tgacatagca ataagtaaat taagtaagac gataacaaat gagatgataa taagtaaatt
     7501 gatatagaaa taagtaaatt aagtaagatg ataacaagtg aggtaataat aagtgaattg
     7561 atatagaaat aagtaaatta agtaagacga taacaaatga gatgataata agtaaattga
     7621 tatagtaata aataaattaa gatgataata tccaaagtga gaaagataat agaaagtaaa
     7681 taaaatcatg taaaaataat ttagataaga ttctaagatt ttatttattt aaaaattagt
     7741 atgattaatt tagattaaat aaatttaata attttagtag gtggtaatga atgaaaataa
     7801 atagattaac agaaataata gtaattctat taaataaaaa attagtaaca gctaaagagt
     7861 tggcagatag atttgaagtt tctacaagaa ccatatatag agatatagaa actctgtcta
     7921 tgtcgggagt accagtatat atgacgaaag gcaaaggagg agggatatct ttaatagaag
     7981 aatattctat agacaaggct atattgtcaa aaaaagataa agagagttta atagttgcac
     8041 tgaaaacatt acaggcgact aagtatccag aaataaattc agtagtaaat aaaattggct
     8101 caatatttgg agagcagaat ttttcaaact ggattgaaat agactttact gaatggggaa
     8161 gtaattttaa tgaagatgat aaatttacaa aaataaaaga agctatttta aggcgtaata
     8221 ctataaattt taattatgta aatagtttgt caagccagac aaacagaact gtcgaaccac
     8281 tgaagcttat gtataagagt aagacttggt acttgtatgg attttgtaaa ctaaaagatg
     8341 attttaggat atttagaatt agtaggataa ggaatttatc tataaaggat gaagtatttt
     8401 caagaaagat aatagaagaa gtatgtttga atgattctaa agtaattaag gaaaatacta
     8461 taacgttgaa attgaggttt aaagaaaaga tgctttttag agtatttgat gattttaata
     8521 aggatttaat aactaaaaat gaagatgata catatgatgt aattacagaa tttcctattg
     8581 gtgaatggat ttatggatat attttatcat ttggtgacaa tgttgaagta cttgaaccta
     8641 aagatgttag ggataatgta ataactagac taagggagtt gtctaaaatt tattcattat
     8701 aatataatgc tagattaaag attttattaa atatataata tcttgcattt agtatataat
     8761 atctcactga gtttaattat attaaaaata tgacataaag gtgtcgtata ttataaatta
     8821 taattgaatt agttcaaaat aaaatagatt aataaaaaca aagtgaggta taaagtatga
     8881 attatgaaat agtagagata aatgagaaaa tagttatagg aatatcgaag gagactacaa
     8941 ataaagatgg acaagcggta aatgatatag gtgaattgtg gaaaaaattt atgggcaaag
     9001 ggatatataa tgctataaag ggtaaaaaga atgacaagac aataggtcta tatacagatt
     9061 accagggtga ttttacaagt ccgtatagct ttgttgcatg ttgtgaagta aatagtaatt
     9121 ctgataaaga gaagaattta gaagaattaa acactaataa tactgttgga gaaagtatta
     9181 ttagtaaagt tataccagca ggtaagtatg caaagtttgt tatagtagga ggtcaaaaag
     9241 aagtaggaga tttttggttt gagttttggc aaatggattt tgataggact tatataagtg
     9301 attttgaaga gtatcaatgt aatacttttg atacaaagaa acaagaaatt catatttaca
     9361 taggaataaa gtagatttgt tattactata ttgaatgaaa tattgagtat agtacattta
     9421 ttatagatag ttttattatt tataactata agcagtaaat ctgtaaaaga tttactgctt
     9481 ttttagtaat taatagtatc agtatcactt tgaatcttca gacatattct tcatttaata
     9541 ttgtttattg ataaatgata cttgtaaata taatgatttg ttatttaatt taaaatatga
     9601 aaaaaagtat tataaacaca ttagaatact tctggattta tgagtataca atttgaaaaa
     9661 tataaaaaat aatgtataaa gctaaatcta atttaatcga taatacatat agagaaatat
     9721 aattaagtaa ataaacaact atctattaga tattgagaaa gtcaaatcct atatgaatct
     9781 acagaaatga ttttaatgaa tctgaaaatt ttttttatac aaacataaaa aaggtataaa
     9841 gttgttttat tataatttaa ttacttttga taacttttta tgttttatac aattatattt
     9901 ttaataactc gtcaaaacgc caaacatata ttacaataga tatataagtt tttttgttga
     9961 caaattatgt taagagaatt aagtataata tggcataaat aattactttt atttatcaat
    10021 gaaatgtatt tattgtgtag cgaaaaatag tcagatagta tagacaataa ctgaagataa
    10081 aagcgtttat tgttgtacat atggaggagt tagttttatg gaaaataaaa ttttgatagt
    10141 agatgatgct atttttatga gaatgataat taaagaagct ctgagtaaaa gtggctataa
    10201 taatttaata gaagcaaatg atggagaaga agcgtgtaat atatttaaat atgaaaagcc
    10261 tgatttgatg ttgcttgata ttacaatgcc aaaaaaagat ggtttagagg tgctaagaga
    10321 aataaaaaag ttggattcaa gtgctaaggt tgtaatgtgc tctgcaatag gacaagagaa
    10381 tatgattgta gaagccataa aacttggagc gttagatttt atagtgaagc ccttcaaaac
    10441 agaaacatta gttaaagttg tagataatgc tttaaaataa aagtgtaaat atacactaat
    10501 acaatgaaaa gcaaataaat gcaataataa agggaggggt ttatattata aattatttag
    10561 aacttgatga tgttcatata gatgctctta gagaaattgg taatataggc tctgggaatg
    10621 caattactgc acttgcatcc atgataaata gcaatgtaga agtattaata cctatggtca
    10681 agatacttga atataatgaa gcgacaaatt tgcttggtgg tccagaaaac aaagtagtat
    10741 gtattttact cgatatgaaa ggtgatatca atggaatgtt tatgtttttg ttggatgaat
    10801 caataactca acttatgcta agttctcttt ttaataaaga agaagctttt ttagatgaga
    10861 ttgaggcaat tgaaatttca gcaattaagg aaataggaaa tattatggca agttcatatg
    10921 taaatgctat tgcatctatg ttaaacatga ctataagtgt ttcaattcca gatatatgta
    10981 ttgatatggt tggagctgtt ctaaatgtac ctatgataag attttcagat gttggtgaca
    11041 aagtcttatt tatagaaaat aaatttaaaa tgagtgataa ttattttact agtcacattt
    11101 tgatgattcc agaaatgagc tcacttagag aaatactcgt gaggttggga ttagaagtat
    11161 gaatagagag atagtagtga acattgcaga tatgaagata gcctatagac caaatgtttt
    11221 agttacttat gcacttggtt cttgtgtagg agtatgtttg atagataaag tagctggaat
    11281 tggtggaatg ttacacgtta tgttgccata tagtaaagat gctattaaca ttgaaaataa
    11341 gtgtaaattt gctgacacag gaataaatga gctcattaaa tctatggaga atataggtgc
    11401 aaatagaata tatataaaag caaaaattgc tggaggtgct cagatgtttt taggaagtag
    11461 caattcagta attgcaagta taggacatag gaatgttgag gcagtaaaaa aaactctttt
    11521 aggattaaat attccaatat tagctgagga cacaggtatg aattatggta gaacaatacg
    11581 tttttacact gagacgggtg aattacttgt agattcaata aataaaggta agaagaaatt
    11641 ataatatata tttcatagaa agtgtatttt aggagggagg tattaaatga gatttccaga
    11701 tgacccatat ttagattatg gactaattga tgaaggaatt aagtatctaa agtttaaagt
    11761 agatgagcaa gattttgggg ttgaacttga aaaggtaata gaaattactg gaaatcaaga
    11821 aattatattt attccagaac tacctacata ttctaaaggc ataataaatc taagaggaaa
    11881 aatcattccg ataatagata tgcgtttgag acttaaaaaa gatgaattaa tgactacaaa
    11941 ttgcatgtat atagtagtaa ctgaaataaa ggacttggta gttggatttg tggttgatag
    12001 agttgatgat atagtaagct tagaaaaaag taaaatatca cctccaccaa gagttactat
    12061 agaatattcg gattattttg taagtggagt tggagaatta gaaggtaaaa taattacttt
    12121 actagatttg gagaaacttt tgacagataa agatgaagtt ttaatagatg aattaatcaa
    12181 tgggtttgat tgcactatca tgtaacatag ggatgtagtt ggagtgttat tataaaaaga
    12241 ttgtgatgag aacatttaag aatatagtgg tataattatt aagtataaaa attttaaatc
    12301 attaaaattt gaatatatta tgtttaagag aggtttgtat atgaaaaata agcaacaggg
    12361 aacaatttta gtcgttgatg atagtgttct gatgtgtgac ttaatagata aaacacttac
    12421 aaaagataat tttaaggtta aaaaagcatt taatgcaata gaggcaaaag aatttattga
    12481 agaatttata ccagacatta ttttgcttga tataatttta ccagataact ctggttttga
    12541 gttttgtaag gaagtaaaat ctaatgctcg tacagcagat atacctataa tttttataac
    12601 ttcaaaaaat acagatacag acatagtaaa aggatttggt gtaggagcaa ttgactatat
    12661 ggtgaaacct tttagtatga cagaattaaa agctcgtgtt atagctcatc ttgaagtaaa
    12721 gaaatctcaa gataaattaa aaaagataaa taatgaactg gaatcttctc ttgaaaaatt
    12781 gaatagactc gttgtaagag attatcttac aggtctttac aataggcgac atataattaa
    12841 tcagcttatg gagcaaagaa gaattaataa atatagtgaa acatcaatat cccttatcct
    12901 tggtgatatt gatgacttta aggttatcaa tgatacttat gggcatgaag caggggattt
    12961 cgtgttaaca gtaatatcaa gtataataaa aaagaattgt agacaaattg atactgtatc
    13021 aagatggggt ggagaagagt ttttgataat gctcacaaat actccaatag aaggtgctaa
    13081 gatactttca gaaagaatcc gtaaagagat aaaggagttt gatttcaact ataaagaaca
    13141 taaaataaag tgcactatta cattaggagt agtagaagta aactcaaata tatcattaga
    13201 agaaaacata tccttagcag ataaagcaat gtatgagggt aaaaacttag gaaaagattg
    13261 tagcgtagtg agtacaatga aaggattaaa aaagatataa acttacttta tgaaggagta
    13321 cagatatgaa aagagaagta aagctttcta aattagcaat tgtattgctg attattatag
    13381 tattggctgt tctagcaggg attttttctt ctatttcatc ttataaagca tttgaaaaag
    13441 tagaaaaaag tattaatgat atgaataatt tgaggactca aagtcagtat ataaaagata
    13501 caactagaga gataagtgat gtattaagaa gatatgtatt tacaggagaa aaaaagaata
    13561 aagatattta tgaaaaagaa ttatacggaa aaacaagaga atcagcttta aacaaattag
    13621 aaaaaattgg tcttacaaag agcgagtcaa ataaaatctt agaagcaaaa gaaatatcag
    13681 attctttagt accaacagaa caaagtgcaa taaaggcagt cgaaagtaat gataagagta
    13741 aagctcaaag tataattttt ggtagtgact atataaactc ccaagagaaa atggattctt
    13801 taataaatga atttcaagag gatatagtta cccgttcaaa ttccaaaatt gtagaagtaa
    13861 aaaaagaatt acaaagtcta atattaagaa caatatttat actttccctt ttaataatat
    13921 tgacaataat agaatataca gtaataaaat taaaaatagt gattcctatt aataaagtag
    13981 aaaaacactt ttcaagaatt gcatctgggg acttaagaac aagtatagac gttgaagaaa
    14041 gtaaatctga aatagggcta ttagtgagtt ctgtcaaaaa gatgcaaagt atgcttttaa
    14101 attacataga ccttatagac agtacattaa ctagtattgc aaaaggagat ttaagagtag
    14161 agatagacca agaatttata ggtgatttta aatcaattaa aatatcttta gaaagtataa
    14221 taaactcttt taatggtgat ttttctgaaa taaatttatc agcagagcaa gttgcaagtg
    14281 cttcagaaca agttgcagca ggtgcacaag ctctttcaca gggtgctact gaacaagcta
    14341 gttcagtaga agaattagct tccacgatta atgagatatc agatggaatt aagaagtgtg
    14401 cagatagtgc tagtcttgca tgtgaggtat ctgaccaatc tgcatcacaa gttggttttg
    14461 gaagtagatg tatggatgat atgatgacat caatgggtga aataagtgaa aaatcgagtg
    14521 aaatatcaaa aataatcaaa acaatagatg agatagcatt ccaaactaat attcttgcat
    14581 taaatgcagc tgttgaagca gcaagagcag gtcaatatgg tagaggattt gcagttgtag
    14641 cagatgaagt taggtctttg gctggtaaaa gtgcaaaagc agctcaaaat acagctattc
    14701 tcatagaaga agctattaaa acagtagaaa atggtgtcag aattgctaaa gagactgcaa
    14761 atgcattgga cttggtagta aatggtgtag aaaaaactac taattatatt aatggtattt
    14821 cttcggctgt acatagtcaa gaagtagcaa ttacacagat tttatctgga atagagcaaa
    14881 tttccatagt agtccaaatg aactcagcaa cagcagagga aagtgcagca gcctcagaag
    14941 agatgtctgc tcaggctcaa attttaaaag aattagtaga aaaatttcat ctaaaggaaa
    15001 gtgaagtaag agaatcatca tgggtttaaa ttatgaggtg aatattgaag taagagaatt
    15061 atcatgtatt taaattatga gatgtatata tattgaaata agagaattat caaggattta
    15121 aattatgaga tgtatatata ttgaagtaag agaataattg tgtatttaaa ttatgaggta
    15181 catatagtga gataagagaa tcatcaagga tttaaattat gagatgcata tataatagaa
    15241 aattatttgt aggtcgtagg tgataaatgt gaattatatt gaaccgagta tgagacaaat
    15301 gttagatatg tttatatttg agacaagttt attactcgaa caattagatg aaattatgat
    15361 tcaaatagaa aaagagaaag tatttactca taacaacatt aatgaaactt ttagaataat
    15421 gcatacgata aaaggttcag catctatgat ggggttagac aatatatctg ctttagcaca
    15481 tagtgtagaa gacttgtttt atgctatacg tgaaggtaaa atagaagttt tagacaatga
    15541 cctattattt gatttagtct ttcaatcatt agattttata agaagagata tagatgagtt
    15601 acaatcaaga ggttgtatat cttctgatta ctctaatttt attgaaggaa ttgaagagta
    15661 tgtgagtact ttggaattat caagttctat gaaatcaaag gataatgcaa atttagaaag
    15721 taaagacgaa aaagaagtaa tcgattcaaa ttttgaatac aaagaagact ctgatgtagt
    15781 aaaaataatc tttgaacaag gttgcatgat ggaaaatgtg agagctttca tgcttgtatt
    15841 tgaacttaga gattactgtg aatttataga tttttatcca aaagatgtag aaacaaatgc
    15901 agagtctaca gagtatatta aaagttatgg gttttatata caatttgtag ctaaagagga
    15961 taaggcaata gtttataaaa ctatagaaga taatctctat gtaaaagaat acataaatat
    16021 tagtaaaaaa gaaatagaaa tgaaaagtac atcaaacaat gatgcagtag aaaataaatt
    16081 aaaagataat aataaagtaa gtaaaagtta tcaaaataac tatacagaag atgagataca
    16141 agaagatgtt ttaaatgaaa aaactgatat ggaaaaagat agtctaagtg aagaaaatga
    16201 acatttacca ataagtaagc agagtattat gagtgtaaat ttagaaaaat tggacttact
    16261 tcaaaatata gttggagaaa ttgtaattat ggaatcaatg gttgtaaata gcgtaaattc
    16321 tacaggtgtt gatatagata atttcaataa ggcaacaaga caattacaca agcttacaga
    16381 tgaattgcaa gatatagtaa tgtctatgcg tatgattcaa attgctggtg tatttcaaaa
    16441 aatgcataga attgtacgag atatgaataa aaaattagat aaaaatgtag agttaataac
    16501 tataggagca gaaacagagg tggataagag tgtaattgat aatttagcgg aaccttttat
    16561 gcaccttata agaaatgcta tggaccatgc aatagagcct attgaggagc gtatagcgaa
    16621 aggtaaagaa gaagtgggaa agatagtttt atctgctgaa aatctaggcg gagaagctgt
    16681 gataagtatt tcagatgatg gtagaggcat tatgagagat aagattttgg caaaagctaa
    16741 ggaacaaggt cttcttaaac atcaaagtaa tgagtatact gatgacgagg tcaattcttt
    16801 gattatgact ccaggctttt ctacaaaaga atctataact gaattttctg gaagaggagt
    16861 aggcatggac attgttcgta aaagtattga aaaagttgga ggttcaattg aagtaaaaag
    16921 taaacaggat gaggggacaa cctttactat aaaaatacca ttaactcttt ctatagtaga
    16981 gggtatgaag tttaaagttg gagattcact atttacttta ccaatatcat ccataagaag
    17041 aacatttaaa cttacagact taaatcaaat aattacagaa ggaaataaga cagagttaat
    17101 tacaatatat ggagcatgta ttcccattat aaggttacat aaaatatata atattcaaac
    17161 aaaagtaacc aatctgatga atggtaatat gataattatt gaaaatgagc ataaagcagt
    17221 atgcattttt gttgatgaaa taataggtaa tcagcaagtt gttgtgaaac catttccagt
    17281 ttattttaac cgatttaata taaaaaataa gggtttatcg ggatgtacaa tacttgggga
    17341 tggaagtata agtttaatta tagatgcaga tagcattata gaaatatatt aggggattta
    17401 aagtatggct aaagatgaaa ttattaaaca agtattaaca ttttatgtca atgatgttat
    17461 ttatggaata gaattagaaa atgtgattga aaccataaga tttcaatcaa taacgtatgt
    17521 accatgtctt ccaacatata taagtggagt tattaatttg agaggaagaa taataccagt
    17581 tattaatatg catataaagt acaatcttcc taaagctgat tataatgaga gaacttgtat
    17641 aataattaca aaggttgatg agtatcaagt tggaattatt gtagataaag ttgttgatgt
    17701 tattcatata gaaaatctaa atttactaga gacgactaat tctaacaaca ctaatattaa
    17761 taaagatata aaagagattg cgaaagttga agataattct atattaattt tagatattcg
    17821 aaagtttttg attaatagca tatagttagt tctgataaat tagcaggagg aagtttttgc
    17881 atgataaaac tcacagatga agaatttata gttcttgtaa accatgtaaa aaaagaatat
    17941 ggtattgatt taagtaaaaa aagagcgtta attgaaggtc gcttatacaa tacaatgata
    18001 gagaaaaaac ttagttcttt tagtcaatat atgaatctat tatttaaaga taggacagga
    18061 aatgaagcta taaacctaat aaatagattg agtactaacc acacattttt tatgagagaa
    18121 cctcaacatt ttgagtttat acagaatagt atacttcctt tttgggaaga aaataataaa
    18181 ataagaaaca taaatatatg gagtgcagga tgttctagtg gagaagaagc gtattcaata
    18241 gctatgactt tggatgatta ttttggatat aataaagagt tgtggaacat taaaatcata
    18301 gccacagata tttcaactaa ctcacttgaa aaagctaaaa gaggtatata catagaatca
    18361 aatgttaaca atgttcctga gttatggaag aagaagtatt ttatagacaa caaggatggt
    18421 acatttaaaa tttgtgataa gattagaaaa agtgtaattt ttaagccgtt taatttaatg
    18481 aatgatttct catatcaaca ttttgatttg attttttgta gaaatgttat gatttatttt
    18541 gatttaaaaa caagagaaga acttataaat aaattttata atgctaccaa aaaagatagt
    18601 tttttgtttg taggacatgc agaagtaatc aacagaagta gtactaaata caaatatgta
    18661 aaacctgcta tatataaacg ataaaaaggt atttttgata tatgattttt tggaggcggt
    18721 aatgtggaac gttttaataa tatggttatt gctataggcg catctgtagg tggtacagag
    18781 gcaatacttg aaattataaa agacctacca aaatcaacac cagggattgt tgtagttcaa
    18841 catatgccag ctatatttac ttatatgtat gcacaacggc ttgacaagca gtgtattatg
    18901 aatgtaagag aagctaagaa taatgataga gtagaacaag gaaatgtgtt aattgcacct
    18961 gggggttacc agatgaaatt gtgtacggat aaacaaggat attatgtaac ttgtgaaaaa
    19021 ggagaaagaa taagtggtca ctgtccatca gtagacgttt tgtttgattc agtagcagaa
    19081 gtagcaggta aaaattcaat aggaataata cttacaggta tgggttatga tggagcaagt
    19141 ggacttttaa aaatgaaaga aaatggttca tttacaattg gacaggatga aaattcttgt
    19201 atagtatatg gaatgccaat ggtagctttt gatagaggtt ctgttatgtt acaattacct
    19261 cttggtgaaa tttcaaactg tttgataaga catattaata ttttaaaaca aaaagattaa
    19321 ttttttataa ataaaaatta atcttttatt aaattatatg gataaaaaac cctagaaact
    19381 ccaaatataa taagagagat ttatatttag gaggaaaatg ataatatgaa attttttaaa
    19441 gtcgatacaa aagctaaagt tttaagatat gtatttgttt taacattgac tataatggct
    19501 gttttggcaa ttaaacatga ctataaatat aataatttaa ggaaagatgc ttcaattaat
    19561 ttaaatagta ataaggaagc agtgactgtt atgagtaacc agtcattacc aaagggtaat
    19621 acaaatagta aacaaacttt atcagtaagt acaataggaa gtaaaacaat agtgaaaaat
    19681 gatgagtatt tagaaagtac tataaacatg cctgtaataa caaattcaaa taaaattgtt
    19741 gaaaggtcta caaatgataa gataaaaaat gacatatttg aattttataa taaatcatat
    19801 aaagaagcaa agcagtatct taaggataat cctgacgaga aaaataagtt tgttgcaaat
    19861 gtagactttg aacttaaaaa gaatactgat tctgctctta gtataaaagt gagatattat
    19921 acttatagtg gtggagcaca tggattttat caagatatag catataatgt ggatatgaga
    19981 actggaaaat ttttagaatt aatggacttg tttaaggata atactaaata taaagaagtt
    20041 atagatgaag aaattaaaag acaaatagct gaacttgaaa aaaaggatga agaaaatatc
    20101 ggaatatata attttaaagg tataaaagag aatcaaaact tttacttgca agatgagaat
    20161 ctagttgtat attttgattt gtatgatata actccatatg cagcaggaat accagaattt
    20221 tcaataaata aagcgttgat aagttctatg ctaaaaccag agtatgtaga tttatttgat
    20281 ttgaaataga agattaaatt aaatgttatg ctataatatt tagcatagag acttattaaa
    20341 attgggggta taaaaatgct acttttaaaa aaagacgaca taaaaaaagt atttacaatg
    20401 agagatgcaa ttgaagctga taaggaagct tttagaattt attgtgaagg aaagagtgta
    20461 aatcctttga gaaccaatat atctgttcca agtcaagatg ctagtatgtt atttatgcca
    20521 ggatacgtag aagaattagg ctgtggggga ataaaaattg tatctgtatt tcctaaaaat
    20581 gctcaaaaag gcaaaccagt aattccagca acagtactat tattagatgg agaaactgga
    20641 gaagttagtg cagtattgga tggtacttat gtaactcaga ttcgtactgg agcagcctct
    20701 ggagcagcta tagacgtatt ggctaaaaaa gattctaaga ttggagcttt gataggtcta
    20761 ggaggacaag gtgagccaca acttgaagcc atgatagaag ctagaaattt ggatgaggta
    20821 agagttttta gtagaaacaa ggaaagtaga gaaaaatttg cagaagaaat gaacttaaaa
    20881 ttaagtaaat ataatactaa aatagttgca gttgaatcat ctgacgaagc catagataac
    20941 gcagatgtga tagttttagc tactccatca aaacaaccag tacttaatgg aaacttagtg
    21001 aaaaaaggag ctttaataag tgcagtaggt tcatatatgc cagatatgca agagttgtgc
    21061 ccaaactgtt taactagagc gtctaagata ttttttgaat caacagatgc agttttatct
    21121 gagtcaggag atatattaat tccattaaaa gaaggtaaga taagtaaaga tgattttagt
    21181 ggtgatttag gaaatgttat aaacggaact attcctggaa gagaagatga tgatgagata
    21241 attgtattta aaactgttgg gataggtgta caagatgtcg taacagcaaa aagaatatac
    21301 gataaagcaa aagaaaacgg tattggaata gactggaagt ggtaaaatat tcaaaataat
    21361 tctatagtta gacaaattta tgttataatg ttgataaata aattttttat tattttaatt
    21421 gagggaggat gtttgtatga aaatggtaaa aaagttaata ttatctacga ttatggctct
    21481 agttttagtt ctaactatag ggtgttctaa tgcagaagaa aaagtaaagt cgaattttga
    21541 agctaattta gctgcattag aaatgtataa gtatgatgaa aaaggaattt ctgatgcatt
    21601 aacagaagct gaatataaac agattcaaac ttataacagc aaggacaagg tttcaatgat
    21661 tgctcaattg acacctcttt atggtaaaga aaatgctgaa aagatatacg aaacaattgt
    21721 taaattaatt caaggtgttg aaaagacagt aacagtggaa tcttctagta aggataagat
    21781 aactttgaaa gttgtaagta aagctttaga tactagtaaa gtaatgagtg aagcgaacac
    21841 tttagtaaaa tcttatgtaa ctgatgaaaa gaattacgat aaaaaagaaa aagaagttca
    21901 agatgatatg acaaaaatga ttatagacct ttacaataag aaaccaacta aggaagttac
    21961 aactatgatt acatatacta aaaaagatgg taaatggcaa ccacctagta atatacaaaa
    22021 gttatgtgaa ggaatatata caatttaatt tattttaaag acacaaaact atcttttaag
    22081 catataataa aatgtttgga agatagttta t
//
LOCUS       DAMJZE010000047        21906 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJZE010000047 DAMJZE010000000
VERSION     DAMJZE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21906
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.047"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            31..1143
                     /locus_tag="PC541_001615"
     CDS             31..1143
                     /locus_tag="PC541_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2839082.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1397..3421
                     /locus_tag="PC541_001616"
     CDS             1397..3421
                     /locus_tag="PC541_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2839083.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3641..4327
                     /locus_tag="PC541_001617"
     CDS             3641..4327
                     /locus_tag="PC541_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2839084.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4422..5477
                     /locus_tag="PC541_001618"
     CDS             4422..5477
                     /locus_tag="PC541_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2839085.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5592..7148
                     /gene="murJ"
                     /locus_tag="PC541_001619"
     CDS             5592..7148
                     /gene="murJ"
                     /locus_tag="PC541_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2839086.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7194..8900
                     /locus_tag="PC541_001620"
     CDS             7194..8900
                     /locus_tag="PC541_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2839087.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="PC541_001621"
     CDS             8922..9995
                     /locus_tag="PC541_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2839088.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="PC541_001622"
     CDS             10007..10729
                     /locus_tag="PC541_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2839089.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="PC541_001623"
     CDS             10745..11902
                     /locus_tag="PC541_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2839090.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="PC541_001624"
     CDS             11905..12744
                     /locus_tag="PC541_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2839091.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="PC541_001625"
     CDS             12759..13934
                     /locus_tag="PC541_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2839092.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="PC541_001626"
     CDS             13963..14712
                     /locus_tag="PC541_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2839093.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="PC541_001627"
     CDS             14734..15642
                     /locus_tag="PC541_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2839094.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="PC541_001628"
     CDS             15690..16442
                     /locus_tag="PC541_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2839095.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16396..17763
                     /locus_tag="PC541_001629"
     CDS             16396..17763
                     /locus_tag="PC541_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2839096.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17769..18860
                     /locus_tag="PC541_001630"
     CDS             17769..18860
                     /locus_tag="PC541_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2839097.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="PC541_001631"
     CDS             18944..20857
                     /locus_tag="PC541_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2839098.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="PC541_001632"
     CDS             21056..21763
                     /locus_tag="PC541_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2839099.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
BASE COUNT         8739 a         2288 c         4051 g         6828 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt tgtgctataa tgaaaaaaga tttacataat attgaattta
     1321 tgtaatataa aacaagctta agatgtatta aaaaagattt aaagatttaa agaaccaaaa
     1381 aattaggggg gatatgatga aaaaaacaac aaaattattg gcaacaggaa tgttgtctgt
     1441 agcaatggtg gcaccaaatg tagctttagc ggctgaaaat actactgcaa atacagaatc
     1501 taattcagat attaatataa atttacaaag aaaaagtgta gtgttaggaa gtaaatctaa
     1561 tgcaagtgta aaatttaaag aaaaattaaa tgctgattca ataacattaa attttatgtg
     1621 ttatgatatg ccacttgaag ctactttaaa ttacaatgaa aagacagatt catatgaagg
     1681 tgtaataaac tataacaaag acccagaata cttaaatgta tgggaacttc aaagcataaa
     1741 aataaatggt aaagatgaac agaaagtttt aaacaaagaa gacttagagt ctatgggatt
     1801 aaatcttaag gattatgatg ttacacaaga gtttataata agtgatgcaa attcaacaaa
     1861 agcagtaaat gaatatatga gaaaaacttc tgcaccagta aaaaaacttg ctggagctac
     1921 tagatttgaa actgctgttg agataagcaa acaaggttgg aaagatgggt ctagtaaggt
     1981 tgtaatagtt aatggagaat tagcagcaga tggaattaca gctactccac ttgcatctac
     2041 atatgacgca ccaatattac ttgcaaataa agatgatata ccagaaagta caaaagcaga
     2101 gttaaaacgt ctaaatccaa gtgatgtaat aattataggt gatgatggtt cagtttcaca
     2161 aaaggcagtg tctcaaatta aatctgctgt aaatgtaaat gtgactcgta ttggtggagt
     2221 tgatagacat gaaacatctt tacttatagc aaaagaaata gataagtatc atgatgttaa
     2281 taaaatatac atagcaaatg gatatgctgg tgaatatgat gctcttaata tttcatcaaa
     2341 agctggtgaa gaccaacaac ctataatatt agctaataaa gattctgtac cacaaggtac
     2401 atataattgg ttatcctcac aaggtttaga agaagcttat tacataggtg gtagtcaaag
     2461 tttatcatca aaaattatag accaaatatc taaaatagca aaaaatggta cttcaaaaaa
     2521 tagagtttct ggagcagata gacatgaaac aaatgcaaat gtaattaaga ctttctatcc
     2581 agataaagag ttgagtgcaa tgttagttgc aaaatctgac ataatagttg attcaataac
     2641 agcaggacct ttagcagcta aattaaaagc tccaatactt ataactccaa aaacttatgt
     2701 ttctgcatac catagtacaa acttatcaga aaaaacagca gaaacagtat atcaaattgg
     2761 tgatggaatg aaagatagtg ttataaattc tatagcatct agtttatcaa aacacaatgc
     2821 accaacagaa ccagataatt caggttcagc agcaggaaaa actgtagtga tagacccagg
     2881 tcatgggggt tctgattcag gggcaacaag tggattaaat ggtggagcac aagaaaagaa
     2941 atatactctt aatacagcat tagcaactac tgaatattta cgttcaaaag gtataaatgt
     3001 tgttatgact agagatacag ataaaacaat ggctttagga gaaagaacag cattatcaaa
     3061 tactataaag ccagatttat ttacaagtat acattacaat gcgtctaatg gatctggtaa
     3121 tggtgtggaa atttattaca aagtaaaaga taaaaatggt ggaacaacta agactgcagc
     3181 atcaaatatc ttaaaaagaa tactagaaaa atttaatatg aaaaatagag gaatcaagac
     3241 aagaacactt gataatggaa aagattacct atatgtgtta agaaataata attatccagc
     3301 aatacttgtt gaatgtgcat ttattgacaa taagagcgat atggataagt taaatactgc
     3361 agaaaaagta aaaacaatgg gaactcaaat tggaatagga atagaagata cagtaaaata
     3421 aaatagaaaa tattaaagca aaacatatca tctcctgtta taatgtaaaa tataacaaga
     3481 gatgatatat ttttgtaaag aatcaaattg tttacaatga tgtttcttgt attgataatc
     3541 tgctcattat tgagtataat atagaaataa gtcaaaaata agatagaaat aagttgaaat
     3601 taagaattaa aaaaattttt attccaatat ggaggtaact atgtctggat atactaatga
     3661 tgagtgtgaa atacctaaaa ttaaaagcta tccaggggca gataaagaaa ttgcaagtga
     3721 gattgactat agcatagtaa aaggaacagt tttattcgac ttgtaccaaa gaattatgga
     3781 tttagtttta tctataatag gacttgtgat aggtttacct ttaatagcaa tatttggaat
     3841 tcttattaaa atagaggaca aaggaccaat aacttataag caagaaagac taggaaaatg
     3901 cgggagaaga ttctatatat ataaattgag atctatgaga acagacgcag aaaaatttgg
     3961 cgcacaatgg gctgaaaaag atgatcctag aataactaag gttggaaagt ttattaggaa
     4021 aactagaatt gatgagattc cacagctgtt taatatatta aagggggaca tgggcttaat
     4081 aggtcctaga ccagagagac cgaactttac tgttcaattt aatgaggaaa tacctggatt
     4141 tatcaacagg ctcgctataa aacctgggct tacaggttgg gcacaagtaa atggtggata
     4201 cgaaatcact cctgaagaaa aacttaaaga agatatttac tatataaaaa ataggagtat
     4261 attacttgac tttaagatac tctttaaaac tgttaaagta gtattgacag gagacggagc
     4321 tagataattt taaaatatta gattactaat caatgaaaaa gctctttaaa aagagctttt
     4381 ttattgtcaa aaaatacaaa aaagaaaagg aatgagaact tatgattaaa aaaatttcta
     4441 ctatattatc gttagttttg ttaatctcta tttctagtac gataggagta tttgcagatg
     4501 caaatcccaa aagagaattg atagaaggga gtataccaga gatttcgaca gaattaaata
     4561 aaagagcatt taaggattct aaagaagtaa ttcttgtaaa tgaagagtca attgtagata
     4621 gtataagtgc aacaccttta gcatatgcaa aaaatgctcc aatagttgta actaagagta
     4681 aaaatcttgg tagagtaaca agaaattatc taaaagaact aggtccagag aaggttacga
     4741 tagtaggtgg tttaaaggca gtatctaaag atgctgaacg taatatagaa aaaatgggaa
     4801 tgaaagtaga aagaataaga gggaaagaca gatatgatac atctttaaag atagctagag
     4861 aaatgtatag aacagtagga tttgatgaag catttttact tagctcaaca acaggattag
     4921 agaacgccat atctgtatat tcttatgccg ctaaaagtgg aatgccaata atatgggcta
     4981 aagatgaagg gtttgaggaa caaattgatt tcttaaaagg caaaaatctt aaaaaaatat
     5041 atgcattagg tgattcaaaa gagtttattg cagagattga ttctaactta aaaaatatag
     5101 aaggaataaa gcaaataaat aagtcaagta caaatgttga tttaataaag aagttttatg
     5161 atgaaaaaga tataaagaaa atctatactg ctagattaga ctttggtagt agatctgatg
     5221 taaacgaata tatttctttg ggagtagtat cagcaaaaga aaatatgcca atactgattt
     5281 gtagtgataa cttaagtcgt gcacaagata aattcttgaa agatagcaat ataaatgatg
     5341 tagttgaagt tgggtataca gtaggtgatt atagtctatt taagagcata tttaatctaa
     5401 cattcttatc ttgtatagta ttaatactat tattactatt gattacattt agggcactta
     5461 gatatgagtc gaaatagttt ttgatttata aggaagcttt tcataggtta aatttactta
     5521 attagaataa tgtttataag cagttttaaa agttctactt ttagagttta aattagataa
     5581 ggagaaagaa gatgtcaaaa acagctaaag cagcattgtg gattatggca gccactatgt
     5641 tttcaaaagt attaggtttc cttagagaac ttgtacttgc aaatttctat gggacaggta
     5701 tgtatgcaga cgtatttgta ttaacattaa atatacctgg tttaatcata gcagtcatag
     5761 gctctgcagt tgctacaact tatataccta tgtactttga gacaaagaaa agactcggag
     5821 atgaaggtgc cttaaaattt actaataatg ttttaaatat atgctatata atggctatag
     5881 taatagctat tattggtctt ttatttacag agcaatttgt tacagtattt gcagcaggat
     5941 ttagaaacga ccctgctaag ttccaagcag caatattatt tactaaaata atgatttcag
     6001 gagttttatt cctgagtgga agtaagatat ttagttcgtt tttacaggtg aatgacagtt
     6061 ttgtaattcc tgggcttata ggaatcccat ataatattat aataatagca gcaatagcac
     6121 taagtgcagg taaaaatgta tggatactgc cagcaggagc actattggct atggcaagtc
     6181 agctattgtt tcaactgcca tttgcgttta agaagtccta caagtataag ccatatataa
     6241 atttaaagga tgagtccata aaagagttag tcaacctagt attgcctatg ttggtaggag
     6301 tagctgttgg acagttaaat atatttgttg accgattatt ggcaaccact ttgggtgatg
     6361 gaaagttgtc tgctcttaac tatgcaaata ggctaaatga gtttgttatg gcattatttg
     6421 ttacatctat aataacagtt atatatccta aactagcaaa aatgtctggt aaagataata
     6481 aagaaggatt tataagtact attgtaaaat cttcaaattg tattatattg gtggtgcttc
     6541 ctatatctat aggagctatt atattagcag agcctcttgt tagaatatta ttccaaaggg
     6601 gtaaatttga tgcgttatca acagacctta catctatagc gcttagatta tattcactag
     6661 gtttattggc ttgtggagta agagatgtct tatatagggc attttactct ctttcagata
     6721 caaaaacgcc aatgataaat ggaagtatag ctttaataat aaatatagtt cttaatttaa
     6781 tattgataag accattgggt catgcaggta ttgctatatc aactagtaca tcaaacataa
     6841 ttacagttat attattattt atatctctta aaaagaaaaa tggatatttt ggaggggata
     6901 aaattataaa gactggtcta aaaagtttgg tagcatctgg tgtgatggca gtagctactt
     6961 tattgatata taataatcta tatgcattta tgggttcagg aacaattaaa gaaattatat
     7021 cagttggagc tggagtatta ggtggagctt ctgtatatac tgtattgata gttttattta
     7081 aggtagaaga gatggattta gcttttgaat ttttaagaaa aggaaaacaa aaactactta
     7141 gaagatagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatacaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaagca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa gccagtaaga aaagtcttaa atgaatgtgg ctttgaaaat gtaatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa caatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggt tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat actatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tactagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgac aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcagcat gttttttaat
    11101 ggtagctata ttattattta atctatttcc ttcattgata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg ctcctaaaga cattaccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagacta tacaagattt gatgagaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatttt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta tagaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagtaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggttcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaggaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa taggatatgg tggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaag ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaacta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagcgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa tccagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aataatccac aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaattg cttagaaaat ataaaaataa
    19981 attagattta aaaacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatt attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtacttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat ataaatgggg agcaactgga ccaaattctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca cgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttg
//
LOCUS       DAMJZE010000048        21593 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJZE010000048 DAMJZE010000000
VERSION     DAMJZE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.048"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            61..1368
                     /locus_tag="PC541_001633"
     CDS             61..1368
                     /locus_tag="PC541_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839100.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1454..1716
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1842..2645
                     /locus_tag="PC541_001634"
     CDS             1842..2645
                     /locus_tag="PC541_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2839101.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2683..3294
                     /locus_tag="PC541_001635"
     CDS             2683..3294
                     /locus_tag="PC541_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2839102.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3326..3997
                     /locus_tag="PC541_001636"
     CDS             3326..3997
                     /locus_tag="PC541_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2839103.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4296..4658
                     /locus_tag="PC541_001637"
     CDS             4296..4658
                     /locus_tag="PC541_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2839104.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5094..5795
                     /locus_tag="PC541_001638"
     CDS             5094..5795
                     /locus_tag="PC541_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2839105.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6066..6884
                     /gene="larE"
                     /locus_tag="PC541_001639"
     CDS             6066..6884
                     /gene="larE"
                     /locus_tag="PC541_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2839106.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            6939..8087
                     /locus_tag="PC541_001640"
     CDS             6939..8087
                     /locus_tag="PC541_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2839107.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8223..9611
                     /locus_tag="PC541_001641"
     CDS             8223..9611
                     /locus_tag="PC541_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2839108.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            9640..10338
                     /locus_tag="PC541_001642"
     CDS             9640..10338
                     /locus_tag="PC541_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839109.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            10341..11774
                     /locus_tag="PC541_001643"
     CDS             10341..11774
                     /locus_tag="PC541_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2839110.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            11796..12533
                     /gene="truA"
                     /locus_tag="PC541_001644"
     CDS             11796..12533
                     /gene="truA"
                     /locus_tag="PC541_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2839111.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    12644..12851
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13104..14303
                     /locus_tag="PC541_001645"
     CDS             13104..14303
                     /locus_tag="PC541_001645"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2839112.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            14550..15596
                     /locus_tag="PC541_001646"
     CDS             14550..15596
                     /locus_tag="PC541_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2839113.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            15704..16867
                     /locus_tag="PC541_001647"
     CDS             15704..16867
                     /locus_tag="PC541_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2839114.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKLNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(16956..17336)
                     /locus_tag="PC541_001648"
     CDS             complement(16956..17336)
                     /locus_tag="PC541_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2839115.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(17339..17998)
                     /locus_tag="PC541_001649"
     CDS             complement(17339..17998)
                     /locus_tag="PC541_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2839116.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(18104..19078)
                     /locus_tag="PC541_001650"
     CDS             complement(18104..19078)
                     /locus_tag="PC541_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2839117.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(19118..19465)
                     /locus_tag="PC541_001651"
     CDS             complement(19118..19465)
                     /locus_tag="PC541_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839118.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(19683..20639)
                     /locus_tag="PC541_001652"
     CDS             complement(19683..20639)
                     /locus_tag="PC541_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2839119.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            21084..21452
                     /locus_tag="PC541_001653"
     CDS             21084..21452
                     /locus_tag="PC541_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2839120.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         8540 a         2207 c         3577 g         7269 t
ORIGIN      
        1 tatataaatt aaattatata atatattgag ttttacataa aagcaaaggt gggaataaaa
       61 atgaaagaaa ttgctgatgc tataacatca gagtatttaa gctatggtat caatgaagat
      121 attagtatct atgaaggtag attttgcata tatttagata aaaaatatag atgtaatggg
      181 aaaatttatt ataaaatgac acctccaata tctataagtt ttaaagcaga cataggttgt
      241 gttgaagaga tagacaatga agatgacaat ttagctttag attatgataa tgctattctt
      301 gaagttcatg gatataaaat tatttctata acaataaata ctttaagtga atttagtgta
      361 gaaggttata tcaatgatga ctgtataaaa tctaagaact cctatgttga atatgtagat
      421 tttaatataa taaacttaga taaaatacct ggaaaactca ttaaatacaa tgataaggta
      481 tacgcaggta gaattgaatt tgatataaat gattatgttg taactataga caagcgatat
      541 gattatagaa aagaattaaa atcagaatta aagtctaaat ctggagcaat aataactcat
      601 ataggcagga ttagaagaaa agatggtaga atttttagaa caaataatac aataaatctt
      661 ttagatagga tatcaacagc tttgagtttt atgtgtggaa gatatgtagg gttttgtttg
      721 gccaaagggt atagaagtgg taatgaagtt tatagaatat ggaatgaaaa tcaaatatct
      781 ccttttagat atgtaccaac atggtcagat acactttcaa attatcataa tatggaaaaa
      841 tatataagcc ttatgtgtaa aaagctagaa gatttttatt atggttctgc tataaagagt
      901 gttgttgatt ggtacataga atctcttgga agtgctacca tggaaaacaa tataatatct
      961 gtacaaattg cacttgaaac tttgtcatat gtaattctag tggaacaaaa taagatactt
     1021 acagatgaag catttgactg taatttggca tctaaaaata taagactctt acttgataca
     1081 tgtaagatac catatggtaa acatgaatta aatatatttg ataatataat aaaaaataaa
     1141 tttgatgatg gagtggattt agttatttat ttaagaaata gcatagttca tccaagtaga
     1201 aagactcata gagctgtgtt agaggttgaa gatatttgga atataatttc aataggaacg
     1261 agatatattg aacttgtttt actctttatt ttaggttata gaggagaata ttcaaacaga
     1321 ttagtagaaa gatgttatgg tgaagtggaa gttgtacctt ggaactagat ttttatattt
     1381 attttaaaat atatattgac atatattgcc ttatgctata aaatcaatat cataataata
     1441 attcagaata acattaaatg ctaagaaaag aaatagtaaa agtagttaat cttaaacaga
     1501 gagtttccga ttggtgagag gagcaataga ttttactttg aacacatctt tgagcaggac
     1561 tctgaaagag tagaaatact ttattaggag atttccggaa tccttgcacc cgttatagtg
     1621 ctagagtata ataattttat tgtacttaaa gaggttagtg ccgtgaggca ttaatgaaca
     1681 gaggtggaac cacgagagtt aactcccgtc ctctagcaga agctagaggc gggagttttt
     1741 taatataatt ttttaagcgc gcagagaatt atattgcaat agatacactt tttaaaaaat
     1801 aatcaaaata ttttaaaaat taaaagacga gggagaatat tatgaaaaat atattaaaaa
     1861 aagtaggaat atttacaatt atgttaggtt tattaggtgg agttgtaggt tgttcaaaac
     1921 cagataatga aaaagataag gatgcttcaa aggaatctaa aaaagaagta gttgtagggt
     1981 ttgataatac gtttgtacca atgggatttt tagatgaaaa gggagataca gtaggctttg
     2041 acgtagactt agctaaagaa acatttaaaa gacttggaat ggaagttaag tttcagccta
     2101 tagattggtc aatgaaggaa actgaattaa atgattcaaa aactgtagat gtattatgga
     2161 atggatattc tataacagac gaaagaaaga aaatagtatc atatacagaa ccatatttac
     2221 aaaataaaca gataatagtt actctaagtg actcaaaaat aaattcaaaa gctgatttaa
     2281 aagataaaga agttggaaca caacaaggtt caacagcact tgatgcagtg gaaaaggata
     2341 aagattttat gaatagctta aaaggtggag caccagttct ttatgatact tatgataaag
     2401 cacttagaga tttagagata ggtagaacta gtgcagttgt tggtgatgaa gttttaatta
     2461 gatactatat gggacaaaag ggtgaagaca aatacaaggt attaaaagac gattttggat
     2521 tagaagatta tgttgtagca acttctaaag aaaatccaga actttgtgag aaaataaatg
     2581 aaacattaaa agaaatgaaa aaagatggaa cttttgacaa gatttatgat aagtggttta
     2641 aatagaagat aagttagctg ttaagaaggg ggcactaaag gcatgttaca aggtttagag
     2701 atagttatag caatgttttg tataacattg atagtatcaa ttccattggg gattggagtt
     2761 gcatttttaa gattatcaaa gaataaatta gtaagtggta ttacacaatg ttatatattg
     2821 ataatgagag gtacaccttt acttttgcaa atgatagtca tattttacgg attacctctt
     2881 ttaggaattg tatttgacag atttacagct ggagtagtag ctttcttctt aaattatgca
     2941 gcatattttg cagaaatatt tagaggtgga atacagtcaa tcgatagagg acaatatgaa
     3001 gcttcaaaag tactaggatt tgataagttt actatgtata agagagtaat ctttccacaa
     3061 gtttttaaaa gaatactagc acctatttca aatgaagtaa tcacattagt aaaagataca
     3121 tctttagtat atatattagg attaaatgat attttaagaa tatctcaaat agcaatgaac
     3181 agggaagcaa gtctgttacc attatttgag gcaggagcga tatatcttat atttgtagct
     3241 atacttacta agggatttga attacttgaa aaaaaatatt cttattatag ataaagttac
     3301 aatattatag gaggaggttt tttatatgtt gaaaatcaaa aacttaaata aatcatttaa
     3361 aaaaaataga gtacttaaag atatttcttt tgagttagaa gaaggtcaga ttggagtttt
     3421 acttggtaag tctggtgcag gaaagaccac aatattaagg tgtataaatg gtcttgaaga
     3481 atttgatagt ggagagatta taatagataa tgaggtaata aaaaataaga gagatatggc
     3541 aaaaatacga ggaaaaatag gtatggtttt tcaaaatttc aacttatttc ctcatatgac
     3601 agtccttgaa aatataattg aatccccagt taatgttttt aaagttccta gaaaagaggc
     3661 tgaagagagg gctagagaac ttttaagatt agttgattta gaagataaat taaattctta
     3721 tccatttgaa ttgtcaggag gacagcaaca gagagttgct atagctaggt catgtgcatt
     3781 gatgccaaaa gtcctttgtt ttgatgaacc tacttctgca cttgatattg atactattca
     3841 aagagtagtc aatataatga acagacttaa agacaaaggt atgacaatat taataattac
     3901 acatgatgtt gtcttctcaa ataatgttgc agataaaatt ataagtataa aagatggtat
     3961 agtagaaaat gttcaaataa aagaaaaaat tgtttagagt tttataaata taaactaggg
     4021 tagaatttta taatataaat aaagggcaag taaaatcaaa tatactatgc ccttttgtta
     4081 tctcaaaatt taatcttata ttttatgttt aagttacatt ataatttata ataaaaataa
     4141 aaggactatt taaatatata atgtgaataa atgtttaata atttgaaata tgataatttt
     4201 ttatgttgaa agcttataat gattatgata taaattggtt atagtataca atttatgggt
     4261 ataacaataa aaaattagaa aggaattttt ataaaatgat taaattatat ggatatacaa
     4321 aatgctctac tgttaaaaaa gctaaaaatt ggcttaagga aaataattta gaatttgaag
     4381 atatagatat ggttcaaaac cctcctagta aagaggagtt aaaatcaatt tataagacta
     4441 gtggatatga tataaagaaa ttttttaata caagtggtat gaaatataga gaattgggtc
     4501 ttaaagatat tgtaaaaact gaatctgatg ataaactttt agaaatattg gtaagtgatg
     4561 gtatgcttat taaaagacca ttacttttgg atggtaaaaa tgtattattg ggctttaaag
     4621 aagatgtatg gaaaagtact ttgctaaaag aagattagga agagataggt gtgttgatta
     4681 aaattattaa ttaatacacc tataaattta tgtattaaat ctaattttta taaatattaa
     4741 ttagcaaatt caaatcaaaa aaattcatat caacaaacat attacttctt attacaaatt
     4801 atatcatatg ttttatatta aaatattata atattacaac gaattgttac aaaattactt
     4861 attattaacc atgaacattt gttttgtgct atcatattta tataagggaa ttgtcataaa
     4921 aaatatcttg ttcatacagt tttatataga aaaaagaaat gaatcttttc tttatataag
     4981 tatattttgt aaattgcctc tatatttata gtttaaataa tagtggtatt aaaaatctga
     5041 caacaaaaat aaaactttca aaaaattaaa tctaaaaaga ataggagata aatatgagta
     5101 ttgaagattt atatgatttt gaatgtgttc ctttgaacaa tgagattgat attgatgttt
     5161 tatttgaaaa gatgatagaa gatggaaaag aaaaagaata tacaccaatt atagtcattg
     5221 atgaaaaagt agggctatta agaaaaaata tagatatgat tactaaagag tatggttctc
     5281 ttgaaaacta tagagagtat tgtttaacaa aatataatga aattgatgta aatcaatttt
     5341 ttgattttag gaagagtgag atttcagaat tattaaagga gctaattgat ttagaagatg
     5401 atttttatga ttataaacat tttagaccaa ttatgaatat agatatattt catgaatata
     5461 acaaagttta catagctaaa attccaacaa ttaaaccata tgaagtattt gcatacatac
     5521 ctattggcgg atttaatgat tgtcctagag atgaagaaca tatagctatt gcaaaatatt
     5581 ggtatgaaaa gcatggaatc tttccaatcg caattggatg tgatacagtt caatattctg
     5641 taaaaaattt aactaaagat ggtaaaaaat ttgatgaatt atgtattgaa ttagtatttt
     5701 attgtgagga tattattata caagggtatg aaaccttaaa ggctcttaag gatgtattaa
     5761 aacgctctac aatagtgctt ttttggtggg attgatattc catcatttag atagtattaa
     5821 attgttagtc accaagaatc cacttgcttt agttatgggg agtgtgtcaa actaggtttt
     5881 tatacacagt gtagcatagt aatatgttta aattacaaag gttaaaaaaa tactttaata
     5941 tttttaatat tataattaat agatttaaag gtaattataa tttataaaat aaatacttta
     6001 tatgttataa tcattaagaa tgtaaaaaaa taatatatag ttttatagtt catatttttc
     6061 cagatatgga ggtaaatttt gtgaaggaaa aagaaaaatt agataaatta aaaaaaatgt
     6121 tacttgaatt aggcagtgtt gtagttgctt attctggtgg agtagatagt aattttttgc
     6181 ttaaggtcgc aaaagataca ttaggtgaaa atgttatagc tgtgactatt catgcaatga
     6241 tgcattcaag tagggaaatt gaagaggcta aacaatatac tcaaaatttt ggtgttaaac
     6301 atatcatatt aaatatagaa aactttgatt taaaagaatt taaagaaaat ggtgttgata
     6361 gatgttatca ttgtaaaaaa tatatattct ctaaaataaa agaggtagca aaagaacata
     6421 atataaagta catagtagat ggaacaaata tagatgattt aggagactat agacctgggc
     6481 ttaaggcatt gagtgaatta ggagtaatta gtccattaaa agatagtagt ttaaaaaaag
     6541 aagaaattag gtcgctttca aaaacattag gattaaatac ttttaataaa ccatcatttg
     6601 catgtcttgc gagtaggata ccttatggag ttgaaataac agatgaaaat ttaagaatag
     6661 tagaaaagag tgaagaatat ctatccaatt taggtttttc tcaatttaga gtgagaatgc
     6721 atggagatat agctagaata gaagttggac aggaagaact aggtaaattt tttgaaaata
     6781 acaattttaa taaagttgat actaaattaa aaatatttgg ctttaaatat gttacactag
     6841 atatgtcagg atataaaatg ggaagtatga atttaaatgt gtagattatt ttaaaaataa
     6901 ataagtttta tagttaatat aaggaaacag gaggttttat gaaaaattat acgttaatat
     6961 cgccatgttt ttttggaatg gaaaaaatgc tggctagaga gattactaat ttaggatatg
     7021 aaataataaa aacagaagat ggaagaataa catataaaac tgatgagttt ggaatagcaa
     7081 agtcaaacat gtggttgaga tgtgctgaaa gagtacatct taaaattgct gaatttgagg
     7141 caaaaagctt tgatgaatta tttgaaaata caaaaagaat aaactggtct agatatatac
     7201 cttatggagc tcaatttcct atatcaaaag cttcctctat aaaatcaaaa ttatacagta
     7261 caccagatgt acaagccata gtaaaaaagg ctatagtaga aagcttaaag aaaagttatt
     7321 tggaggatgg tttgcttaaa gaagataagg agaaataccc tatttttgta tttatacaca
     7381 aagataaagt aaccatttct atagatacaa caggagatgc tttgcataag agaggatata
     7441 gagaaaaggc aaataaagct ccaataagag agactttagc tgctgggctt atttatttaa
     7501 ctccctggaa agcaggtaga gttttagtag accctatgtg tggttctggt actatattga
     7561 ttgaagctgc tatgattggt ataaatatgg cacctggctt aaatagagag tttatatcag
     7621 aaaagtggag aactcttgat aagaaaatat ggtgggatgt aagaaaagat gcttttaata
     7681 agatagataa tgaatctaaa tttaagattt atggatatga tatagatgaa gaatctatag
     7741 atatagctag agaaaatgct gaaatagcag gtgtagatga atatatagag tttaatgtag
     7801 gagatgcaac tcaatttaaa agtgaagatg agtttggatt tataatcact aatcctcctt
     7861 atggagaaag attagaagat aaagacagtg ttaaacagtt gtataaggag cttggttatg
     7921 catttagaaa attaaaaaat tggtcgtatt atttgataac ttcttatgaa gactttgaat
     7981 atgagtttgg gcaaaaagca gataaaaaaa gaaaattgta taatggtatg ttaaaaacta
     8041 actttttcca atatccgggt cctaagcctc ctagaaataa taaataagtt ttacatgatt
     8101 tatttgtaaa atataaaatt ttattaaaat aagtaaaaca ttatgatgcc ttgaaacgtc
     8161 taattattgt gaggagattt ttgtgaacaa atttaaagat ttataggatt aggaggagtt
     8221 aaatgaaagt gttaaagctt attactgtag tagccctgat gatgttattt gttactggat
     8281 gtaatataaa aactgaatct ccagaacaat tagcgaagat acctgactat gatgatacaa
     8341 aaaaagtatt gtatgatggt attgaccagt tactaaaacc agattcatct atgatattgc
     8401 cttctaatac aaaagaagta ggaaaaataa ataaggtaga tttaaacagc gatggaattg
     8461 atgaattagt agtatttgaa caaaaagaag atttaagtaa taatgtgagt caggtagggt
     8521 ttgtaacctt gagttacaat ggagaaaaat acgtacttgg agaccatttt ttagaaaatg
     8581 gcgaatcaat agaatatgct aatttttatg atttggactc agatggatat aaggaagtta
     8641 tcttattggt taaatccaaa gacaaaacga atatgcatat atataaagta gtagataatg
     8701 aaataacaaa aatatatgac ttggatgcat catggcttca aaataaagaa gattttaatg
     8761 atatgaaagt aaaaattgga tatatagatg gagatgacaa gttagacata ttgatgcttc
     8821 atttgaataa taagactaat gaaatgtttg caagtgttgc aaattttgat gggaaaatga
     8881 aaatcaaaga ttctgttaag tttgaaaatg taaaaaactt aagtgaatta tatataacat
     8941 taggtaatgt tgcttcatct gttggaaatt cttctgcagc tataaaaggt attgtacttg
     9001 atattcctat attgaaagat aataactata ttacgcagat actttatatg aaagatggga
     9061 aaattaacaa agcttttagt gattatgata aaactattac taaatcttat tatatacctg
     9121 tagatgatat tgacaaagat aagattatag aaatacctat agttgcaggc agtacaggta
     9181 ataataaaaa tacttatagt tctaaatcat ctgcaactat aagttggtat agatggaatg
     9241 gaaaagaagg tgctgactca agtttaatat ttacaagtca gatatattat aattataaat
     9301 ataattttaa attatttatt ccaaataatt tatttgataa aatacttgtt gaacaggaat
     9361 ttgttggaga aaaagcagta tttaaatttt actattttga ctattcagac cgtaagaatt
     9421 tatttaatat agtagtagag agtaaaaata aacttgaaga tagaaaaaat acaggtactc
     9481 aaaattctat tgtacttcaa gagagcgatg aatatacttt cttactagtt gtaaacaatg
     9541 caaatgaaat ggataagcta gatatgacta tagacgcatt aaaagaatat ttctcattaa
     9601 tatatggtta atattatact caattggagg ttttatttta tgaaggaaaa gatacttata
     9661 ctagaagatg aaattggtat tagaagtttt gttagtataa acttaaaaag ggaaggctat
     9721 gaaatagttg aggcaggtac tggaagagaa gctatagaaa aaatgacaac agaaaaggat
     9781 attacaatag ctcttttaga tgtaatgctt ccagatataa gtggtataga agtatgtaaa
     9841 tttataaggg agaattttga ccaagtaggt ataataatgc ttactgctaa agctcaagaa
     9901 gatgataaaa tagaaggatt tatctcagga gcagatgatt acatcataaa accatttagt
     9961 ataaaagagc ttttagtcag agtttctgct cttcttagaa gagtagcaaa ggatgattca
    10021 agtgttaagt cgagtgagat agtttctcca ccatttatac ttgatataga caaaagaaaa
    10081 ttatttaaaa atggaaagga aattgagctt acaccaactg aattttcaat agtgaaatat
    10141 ctaatatcaa atgctaagca gtcgttaagt agagaccaga tattagacga agtatgggga
    10201 actaactatt tatatgattt taaaatagta gatgtaaata taagaaggat aagaaataaa
    10261 atagaagatg acccatcaaa accaaaatat atacaaacta tttggggtta tggatactgt
    10321 tttagaaagg aagaataata gtgttacatt ttgaaggtct gagaaaaaaa gtaataaaaa
    10381 actattttat aataattatt ataatggtta ctctgtttga aggattgttc atgttttaca
    10441 tacaaaacta ctattatgat tctgtcaaac agctacttga atctgaaata aaatatgctg
    10501 atgaatataa tgctattaca atggaaacta caagttttga gaaaaaagta aaaaatatat
    10561 ttgataaaca acccttaact aaaaattcag aatttggaat ttcaataata gataaagata
    10621 aaaatattat attagaccaa tatggtttta aaagtaaaga aaaagcaaac tatgaagatg
    10681 ttaacaatgc attaaaagat ataaaaacta aaaatttaac accttatact tatagaatac
    10741 cagatacagg ggagcatgtt atgagtatct ccttaccact taaggtaaat aatataatag
    10801 aaggtgttgt tagatataca gtatcattag atgctattga taatgcaata ttaaagcaag
    10861 ctacatggct tatattagca ggaatattta tacttattat agctatacta ataagtctta
    10921 aatttgctga gactcttata aaaccattac gagagctaaa gaaatttgca aatgaattgg
    10981 ctgtaggaaa ttataatata aaattagaaa agatgaagat tgtagatgat gaaattggtg
    11041 atttagcaca gacatttgaa cacatggcac atgagattga caaaagtgaa aaattaaaag
    11101 aggaatttat atcatctgta tctcatgaac taaggactcc tcttacatct ataaaggggt
    11161 ggagtgaaac cttaggttat gaaagtataa caagagaaga actagactta ggtttaggca
    11221 ttatacaaga tgaaacagaa agacttataa aactagttga agaattatta gacttttcaa
    11281 ggctttcttc agacaggata aaacttcatg ttgatatagt tgatgttgaa ggtttaattg
    11341 taggtgttgt aaatcaactt aaagttaaag cagctgaaaa agatatatca cttttatttg
    11401 aatttgaaaa tgaatttata gagaacattc aaggagataa aaatagatta agacaggttc
    11461 tcataaattt aatacaaaat tcatttaaat ttacgagtca aggtggttac ataaaagttg
    11521 tagcttctca agatgaagag ataacaacca tatcagtaga ggataatggt tcaggtatag
    11581 aaaagcaaaa tttaaataaa gtgttagata aatttttcca agaagattat aataaggctg
    11641 gtagtggatt aggactagca attagtaatg aaattgtaaa acttcatggt ggaagaatga
    11701 aaatagaaag tgaaaagaat gttggaacaa aaataacttt taatataaaa aataaatttg
    11761 ctaaacaagc ataaattctt agaaagagga ttaagatgag aaacataaaa atgatagtag
    11821 catacgatgg ttcaagatat aaaggatatc aaaaattagg tgacaataat atgactattc
    11881 aagaaaaact tgaaaatgtt ttaagtaaaa tgactaatga aacagtggag ataataggtt
    11941 ctggaagaac tgatatggga gctcatgcaa gaggacaagt ggtaaatttt agaactaatt
    12001 gtatggattc tttggataaa attcaaaaat atttatatga atatttacca gaagatatag
    12061 ttgtaaaaac tgttgaagaa gttgatgaaa gatttcattc aagatacaat gtaaaatcaa
    12121 aaacttatat gtataaaatt gacaataata agtatcatag tccttttata agaaaatatg
    12181 ctactcatgt aagtaaaaaa ttagatttag atagaatgag aaaagcaagt gagtatttag
    12241 taggagaaca tgattttacc agttttgctt cttctaaatc taagaaaaaa tcaaatgtaa
    12301 gagaaattta ttctataaat ataaaagaag atgataatgt tatagaaata tatgtagaag
    12361 gaaatggatt tttgtataac atggtaagga taatagtagg tgctttaata gatgttggtc
    12421 taaaaagaag agctccacaa gatattaagt atatgcttga atcaaaagat agatgtcaat
    12481 catcagatac tgcaccagct aaaggactat gtctttggaa agtaacatat taatcaatta
    12541 tataaaactc tctttaaaat aaaaataaaa taaattatat agttgacata aatggaaaaa
    12601 taaactatta tatattttat aagagagata taaaacttta tatttaaatc ctgtgagaaa
    12661 gagagtaact tctatgtgtt ttatagagag ctgaggatgg tggaagcttg gcaaataagt
    12721 attagtgaaa agaactttgg aggatactaa ttgaatttag tatgttagta ggtaatctcg
    12781 ccttaagaga aagagtggat aagtttatct tatcaattag agtggtaacg cggatatatt
    12841 tcgtctcttg gtttttccaa gaggcgtttt ttaatatatt taattattag tgtttatctg
    12901 gtatgttgaa catttactaa ttgattagct aagtttatca ataagagtgg taccgcggat
    12961 atttcgcctc ttagtatttt aaaatactaa ggggtttttt tagtattaaa atgattttat
    13021 gtgagtctaa attctcagag tcattcaata ttaagaattt ctataaaata aaaattataa
    13081 tttaagattg ggaggaatta aaaatgaagg aaaaagtagt attagcatat tcaggaggac
    13141 ttgacacatc aatcataata ccttggttaa aggaaaatta tgaagatata gatgttatag
    13201 ctgtttgtgg aaatgtaggt caggaagata aaatggaaga tgtatatgaa aaagctttac
    13261 aaagtggagc atcaaaggca tatgtggatg atataagtga agaatttgta actgaaacta
    13321 tatttaaagc cgtaaaagca gaggctaaat atgaaggaaa atacttatta ggaacttctt
    13381 tagcaagacc tataatagct aaaaaattag ttgaagttgc tcataaagaa ggtgcaaaat
    13441 atatatgcca tggatgtaca ggaaaaggta atgaccaagt tagatttgaa gctacaattg
    13501 cagctcttga cccgactata aaagttattg caccttggag aatttgggat ataaaatcaa
    13561 gagaagatgc tatagactat gctgaaaaac ataatataaa ggtaacagca actaaagcta
    13621 aaatatattc agtagatgct aacctttggc atgtttcaac agaaggtgga gatatagagc
    13681 acctagaaaa tgaacataaa aaagatgttt ataaacaatg tgtagaccca gaagatgcat
    13741 gtgatgtagc tgaatatgta gaagtttact ttgaaaaagg tgtacctaaa aaggtaaatg
    13801 gtgaagaatt atctccagtt gcactgattc ataagttaaa tgagttgggt tgtaaacatg
    13861 gaattggtgt tatagatata gttgagaata gacttgttgg tatgaaatca agaggtatat
    13921 atgagactcc aggaggaaca atcctttatg aagcacataa tattttagaa agtgctactt
    13981 tagacaaaga tacattacat ttcaaacaaa tggtatctta taaatacgga gaacttattt
    14041 acaatggact atggtattgt aaattaagag aatctataga tgcatttatg gaacaaactc
    14101 aagataatgt tacaggaaca gtaaaagtta aactttacaa aggaaatata aaacctgctg
    14161 gaatatttac tgaaaatgct ctttatgatg aaggaatatc ttcttttgga aatagtgaac
    14221 tttatgacca caaagatgca gaaggattta ttaatttatt cactttacca ttaaaaatca
    14281 gagctatgaa agctggaaaa taataaaata aattatttaa gagtgtataa aaactatttt
    14341 taggttttta tatgctcttt ttttatttga atttattaac ttatacagta aatagtataa
    14401 aaaaatatat tatagttcca aatggcttaa taatgtgaaa atataagacc taaaatataa
    14461 attttataaa tacaatagtt attgactctc tcattgtaag agggtttata ctgaatctaa
    14521 tgaaaattta tagtgaggag atagattata tgagaaaaat aattgaaatt aaacatcatt
    14581 cacatgatta tgagtgtatg tggaatggta tagaagattt atatataaat aaaaccggtg
    14641 aatgccttcc aaacgatttt ttctttttac tgtcaggttt tggttcattt tgttatatga
    14701 aaacaaataa agctgatttg aaaagaatgg tagctttggg agatggaagg acaaaacaga
    14761 tgtataaatt tttagcacca attgttggat ttgaatacaa acatcatgag tttaaaaagt
    14821 atgagcaagc attaaagaaa gcaaaatctg aaattgatga tggatatcca gttattttag
    14881 gagctttaga tatgtattat ctaccatatt ataagaagct atatcataaa gaacatattc
    14941 cttttcatta tatattaatg gtaggatatg atgatgaagg aatatatctg tatgattgtg
    15001 gtagaacaga attattacat ctttcttatg ataaattacg tgaaagcatg aattgcaatt
    15061 cccctggatt aagcaaagaa aatactattt gcacaataag aatgaatact tcaaagaata
    15121 aatataacat tgctagagaa gctattgcta taaagcgtga tatgttttta aatccaccta
    15181 aaggattttt gggatataaa ggttttgaaa agtttattaa agagttgcca cagtggaaca
    15241 ctgaattgga taaagatgat tatgataaga ttttgaccaa tatggttatg ttttttggaa
    15301 cagtaccaac aattccaaat gcaatcaaag gaattaaaga accagatact gttcaattta
    15361 aaggtggatt tgataaaatg agtagaatgc tcaacagtat tggggaggaa tacaaaaacg
    15421 actcttttat acaagtagct gatatttttg ataaaactgc tattataatt gagaaaatat
    15481 ccaatattat aatagattac ttaactcaaa catgtgatga tacaggacaa cttccgttat
    15541 tgttctctga ggtcttagag catatgaaaa caggatattt aatcttggat gtataaaaaa
    15601 tatattgttg accctaacat tacgtaatag tgtaatatga aaatatagct tgaatatatt
    15661 agtaggtatt gatggctgta aaattaaaat ttgaggtgtg gatatgagaa cagtgaaaca
    15721 agtttctgat ttaactggaa taagtgtgag ggcattacat tattatgatg aaataggtct
    15781 tttaaaacta aataaaatta cagatgcagg atatagactt tatgatgatg aatctattaa
    15841 aactcttcaa caaattttat tttttaagga gattgatatt cctttaagag aggttaagga
    15901 aataatgtca agccaatatt ttgataaggt tgaagctcta aaaaatcaaa aaaaattact
    15961 tattttaaaa cgaaagagac tggatgaatt gatagagctt ataaatcaaa ctctaagagg
    16021 agaaggaaat attgatttta aagagtttga tatgagcgag tattttgatg ttttagaaga
    16081 atttaaaaga gagcatagga ataaggtaat taaaatttat ggtagtgtag aaaaatacaa
    16141 tgaatatata gaaagagtta agtctaatga agagaaaatt gctaaaatgg ctataaaaca
    16201 atatggaact attgagaagt ttgctaaagc tataaaaaca aattttagca gtgatatatt
    16261 gaatttagga gaaaaatttg atagatataa aaatgattgt ttaaaagaaa aacatccaaa
    16321 attaaaagaa ttatatagaa agcttgtaga agatttaaat aggaatcctt cttcaacaga
    16381 ccttcaagaa atagcaaaag agataacaga tatatctaaa aaagattatg aaattttctc
    16441 tatggatact ggtgatgaca actggtatta tatggttcaa aattatctag taaatcctat
    16501 gtggatagaa gaagtagata aaaagtatgg aagtggagca ggtaagttta ttggacaagt
    16561 actaaagact tatctaagag atagaaaacc aaagataaat acattgtatg aaaaacttgt
    16621 agaagatttg agtagagatt gttcttcaag ggaagttcaa agcattgttg aagaaataga
    16681 caatgaaatg aaaagaagta atgaatttta taaaatagac aatggtgaaa gatactttga
    16741 ttatatgtca gagctttatt tacaagattc taactatata aaagtaactg ataagaatta
    16801 tggtgatggt gcatctaagt ttattggaga agcttttaag atttattttg ataataataa
    16861 ttgttgaaaa tttggaagta aaaaatatgc aagttttata aaagcacaaa aataaggatg
    16921 tagattagct ccatctatat ccttatttaa agttattaaa atatatagtt tacatcaaac
    16981 ttaacacttt caataatatt tcccaaatat gtttttatac tttcaacttc ttctaaagat
    17041 tttaatttat tttcagtata aattgctttg tccagaatat taaaccttaa tcctatttca
    17101 tcaaagtact catgatttaa aactttgtac caatggtttt tttctttatt ccaactcgta
    17161 gatattgaat cagcttcttt ttgagctttt tcccaatctt cattttctat agatttttct
    17221 aaaacagata tattgtcttt atatccttct gtaaaatcat atagtttatt gttaacataa
    17281 aatccaaata atacaaataa gatagtccat agtataacaa atgttgtaga tttcaatatt
    17341 aatcaccctt tttattttga ataaatattt tgtcattttc atctaaaaca caaataagaa
    17401 catctttaaa agtttctatg tgattagatt ttaaaatacc cattaaccag ttagtatctt
    17461 tttgagctat gtctagattt ttatttatta ttctaccatc cagtataagt gggataggta
    17521 aatgattaaa agctcttgga ggagtactat tgtagcttga tgctggtaca acacttagat
    17581 taccatcagt ctccaaaata gcatattgta catcttttag gttaaagtat ccttgaatcc
    17641 taagttgttc taataattca tctatactaa ttctttcttt tttaagctct ttatatacaa
    17701 tttttccttt atctattaat acagaaggtt tacctgataa aaatgaactt aattttctac
    17761 tttttaatga taagaatgaa attagagtct gcatgattac aagtccagat attgctgcaa
    17821 caccatttat gattggtata ttattatttt ccattgggac agatgcaact tctgcaatta
    17881 atagtgttat aactaaatca aaacagttca tttcagctat ttcacctttt cccatgactc
    17941 taagtgcaat tagcactgtt atgtataata taatacttct tattaatact actgtcatat
    18001 ctatcaatta ctcctttaaa aaatatttac acttagttag tatatcctat ataaaaaatt
    18061 taattcaaag tataaaaaac cttaaataat tggattcaac tatttaaggt ttttttgata
    18121 attttaatat taaatttttt atggattttc tgttactttt atttatttta ataacagatt
    18181 caacaaaact ctttttatct cctaatttta agataaaatc aggatattct tcaaattcat
    18241 tgagttttat taatttgaaa gaagaagata gatttttaaa gttaataatt ttagagttat
    18301 tcactaatac acttcttttt ttaaaataaa tttctttttt acttatattt agtatagtta
    18361 atatttgttt aagatatatt attatatctt ctggagttct atcaaatttg agttcaaaat
    18421 tttcatcttc aaagtaggga atataatata aaggtgtttt gagagcaggt aatttattat
    18481 ataattcttc aagaatatcg atatttttag gggagttaaa gaaaaagcat ttacatacat
    18541 tcattaaatc cacagcatct gaaagtgagt tatgtttaat atctccttct acagaaaaaa
    18601 tttttttcat attcagtaaa gatatttgct tagataagat tctattattg tatgtaatat
    18661 gttgagatat aaaaggttga atgtcaatca tcatattaaa taactctaag catttacctg
    18721 tatatttata tactctaaat gaagttagaa ggccagttat atcaaaatca ccccaattat
    18781 atattgtaac atcatcaaat ttacaaaacc atttataaaa atcatccata acatataaaa
    18841 gattaggagc attattgata tctagttggt tcaatttggt taaattttta catttatttg
    18901 aaagtatttt attgtatttt ggcttaacta aagagtaata attatctatt gtattaaatt
    18961 cgttatcaac taaaactcca ccaatagaaa ttaattccgc accattatat tcttttggtt
    19021 tatttttatt atcatctagt atattaaatt caaagtctat aaataacttg tatttcataa
    19081 atctaaagca catcctcagg taaattattt attgagccta agcatcataa gaatcaatag
    19141 atataatgac tctaaacatc aaaggttcat tacaactttt atttatttca taatttaatt
    19201 caaataaatc ttttaagata taatttataa gtattctttc ccaacccatt tttaaattaa
    19261 ctttagagtc tattaatata ttttctatct ttttaaatat ttctatattt gctggtatta
    19321 tactgagctc aatattactt ttaaacgggt tagactgtaa atctgttata agtatatttt
    19381 ttaattcctc tgtaagttca gaaattaaac tatcacctat gtattttttt atgatttcct
    19441 ctctaatttc ttttaaattt agcaatatta gtacctgcta cttacatagt agcttttcct
    19501 ttccttttaa tactattatt catttttgaa tcttatttag atatatttta agattaaaag
    19561 tctttagcta tataattttt tacccatttt taacttatat taacatatta caggcttata
    19621 tctccaccct gtttacattg aggatggagt atattgccta ttttgataaa aatgataggt
    19681 tgctatttgc ctattccttt ttctgataat ttttctaaca tatctttggt cattttatct
    19741 aaatcataac taaacttaaa gttccattct tttactgcac tactactgtc tagagagtta
    19801 ggccaagaat cagctatact ttgtcttaca gggtctacat catattctat cacaaaatca
    19861 ggaatatatt tttttataga atcagctatt tcctcaggag aaaagctcat agcagtaata
    19921 ttaaaagcat ttctatggat gagtttatca gctggagctt cgattaaatc cactattgat
    19981 tgaagtgcat caggcatata catcatatcc atttttgtac cttctgctat gtaagattta
    20041 tatctcttat tttttaatgc ctcataatat atatcaactg cataatctgt agttcctcca
    20101 ccaggaggcg taacataaga aataagacct ggaaatctaa cacctctagt gtctactcca
    20161 aattttgagt gataataatc acatagtaat tctccagaaa ctttagtcac accatacata
    20221 gtattaggtc tttgtaatgt atcttgagga gtcatatttt ttggagaatt ttcaccaaaa
    20281 gcagcaattg aactaggtgt aaaaaattta caatccaatt ctttggctat ttcaagacca
    20341 tttattaatc cattcatatt taagttccaa gcttctagag gtttactttc agcaactgca
    20401 gaaagtaaag atgctagatg aattatagta tctacatcat atttttttgc aatttcaaaa
    20461 aaacgattct tatctgtaac atctaatatc tcaaatatac cagattcaca tactggatta
    20521 ccttctttta ctctccttga actagcgatt acattttgtt ctccatactc atttctaagc
    20581 tttattgtta attctgaacc tatttgtcct aatgcacctg ttataagtat ttttttcaat
    20641 tttacaacct cctaagggtt taatttatgt agtactactt atttttattt tagcaaatct
    20701 tttgtttaaa tacaaagaat taaagataat aatatacgac ttaattgaat gaagtgcagt
    20761 aagtgcctaa aaatttaaag ctaatatata atggtatatt gtataacaat aaaagagtag
    20821 attaaattat tatatataga ttgtgtgact aagtcacaga aaatagagtt aatttaatat
    20881 ataataaaag catatcaagt aaagctttaa attaaagtaa ctttataaat aaaaagtaat
    20941 ataaattgat aaaattataa atttatttat agtgaatatg ggttggattt tatagataca
    21001 aaaatagtat atatgatatg tagaccatta gcaggtttaa agtagcaaaa catataaaat
    21061 aaaatttgaa aggaagtata aaaatgacaa gaccatcaat atatcatagc caaggataca
    21121 cttgtgcaga ggcattaata aagtcttata atgaggaaca taacacagat ataccaatct
    21181 caataggaag tggtatgggg gttggaatga atgttggaag tgtttgtgga gctgtaaatg
    21241 ctgctgttgt tataataggg tatttaaatg ggcgaaatag taatttagat gaaaatatag
    21301 caaggaagta ttcaagagag ctattgaata gagtaagaaa taaatacagc tcagaaatat
    21361 gtgtatctct taaaaaaaat aaagtaactt gtgaagaaat aataaatttc acatatgatg
    21421 ctctaaatga tatgctaaaa aatcaaaaat aattctaaga aacaagtatt ttacattcga
    21481 tagaatactt gtttttttat gatattttta ataagtatta gtttattata atttttaaat
    21541 caattttatt atattttaat tgaaacaatc tactttgaaa attactaact gac
//
LOCUS       DAMJZE010000049        21421 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJZE010000049 DAMJZE010000000
VERSION     DAMJZE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.049"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            300..1571
                     /gene="serS"
                     /locus_tag="PC541_001654"
     CDS             300..1571
                     /gene="serS"
                     /locus_tag="PC541_001654"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2839121.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1804..3309
                     /locus_tag="PC541_001655"
     CDS             1804..3309
                     /locus_tag="PC541_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2839122.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3423..4121
                     /locus_tag="PC541_001656"
     CDS             3423..4121
                     /locus_tag="PC541_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2839123.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4406..5494
                     /locus_tag="PC541_001657"
     CDS             4406..5494
                     /locus_tag="PC541_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839124.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5733..6923
                     /locus_tag="PC541_001658"
     CDS             5733..6923
                     /locus_tag="PC541_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839125.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7014..8516
                     /locus_tag="PC541_001659"
     CDS             7014..8516
                     /locus_tag="PC541_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2839126.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8588..9844
                     /locus_tag="PC541_001660"
     CDS             8588..9844
                     /locus_tag="PC541_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2839127.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10016..12790
                     /locus_tag="PC541_001661"
     CDS             10016..12790
                     /locus_tag="PC541_001661"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2839128.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(12855..13040)
                     /locus_tag="PC541_001662"
     CDS             complement(12855..13040)
                     /locus_tag="PC541_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2839129.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13415..14593
                     /locus_tag="PC541_001663"
     CDS             13415..14593
                     /locus_tag="PC541_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2839130.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14720..14805
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14923..17841
                     /locus_tag="PC541_001664"
     CDS             14923..17841
                     /locus_tag="PC541_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2839131.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      18027..18112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18212..18874
                     /gene="zmp1"
                     /locus_tag="PC541_001665"
     CDS             18212..18874
                     /gene="zmp1"
                     /locus_tag="PC541_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2839132.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(18996..19958)
                     /locus_tag="PC541_001666"
     CDS             complement(18996..19958)
                     /locus_tag="PC541_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2839133.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20128..21318)
                     /locus_tag="PC541_001667"
     CDS             complement(20128..21318)
                     /locus_tag="PC541_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2839134.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8452 a         2483 c         3672 g         6814 t
ORIGIN      
        1 tatataaaag ttaataacta tacaagattg ataaagaaaa agtcaaaata gataattgat
       61 tagcaaaata gatataatta taatgaagtt aaatctagca atattctcct aaaattaaat
      121 tacatttatt taattaaata taatttgaca atggacaaaa aagtgagtaa aatataaata
      181 aaataaaaat aacaggttcg gataaagaaa aaggtttctt aacctcaagc ataaagtata
      241 ttatctaagt tatttgagat taagagataa atccaatctt aggtactgag gaggaaaaaa
      301 tgcttgatat aaaaagaata agagaaaatt tagattgtat aaaaaaagca atggaaagaa
      361 ggggcgaaaa agactttaac cttgatgaag tagttaagtt agatgatgaa agaagaaaga
      421 ttcttcaaga agttgaagtt atgaaaaatg aattaaatac agcatctaaa aatataccaa
      481 atcttataaa agaaggtaaa gatgtagaaa atgaaaagat taaactaaaa gagttatctg
      541 ataaaattaa ggtaatagac caaaatctta aagaagtaga agataagatg gaatacctat
      601 taatgagaat accaaatgta ccacatccag aagtaccaca aggtgaaact gatgaagata
      661 atgttgaagt aagaacttgg ggaaagacta ctacctttga ttttgaatct aaggctcatt
      721 gggaaattgg aacagaatta ggtatacttg attttgaaac agctagtaaa ataactggtt
      781 caagatttac tctttacaaa ggattaggtg ctagattaga gagagcatta ttaaattttt
      841 acttagacac gaatacaaaa gtaaatggat atacagaagt tattccacca tttatggcaa
      901 atagaaatag ttttttaggt acaggacagt tacctaagtt tgaagaagat atgtttaaaa
      961 tagaaggtat ggactatttt atgattccaa catcagaagt tcctttaact aatatacatg
     1021 ctaatgaaat acttaaattt gaacaattac cgataaatta tacagcttat actccatgtt
     1081 ttaggtcaga agctggttct gctggaagag atacaagagg tttagtaaga caacatcagt
     1141 ttaacaaagt agaaatggtt aaaatagttg caccagaaga atcttacaat gagttagaaa
     1201 agcttactaa caatgcggaa actatgctac aattattgaa tctgccttat agggtagtaa
     1261 agatatgtac aggtgattta ggatttacgg cttcttttaa atatgatgta gaagtttgga
     1321 tgccaagtta caataggtat gttgagattt catcttgttc aaactgcgaa gatttccaag
     1381 ctagaagagc aggaataaga tttaaaagag ataaagattc taaagctgaa tatgtacaca
     1441 cattaaatgg ctctgggtta gctattggaa gaagtgttgc agctatatta gaaaattatc
     1501 aacaagaaga tggttctgtg gttgttcctg aagttttaag accatatatg ggagtttctg
     1561 ttattaagta agagttaaat tatgtatttt taagcaatac aataatatag aggtaataaa
     1621 aaataattaa tataaaagtg atacaataat atagaagtaa taaaaaaaaa taattaatat
     1681 aaaaatgtgt acaaactaat gaatattgat atagtaagct atcatatgaa agagtatgta
     1741 cacatttttt ttactaattt ttcacaatat gaacataaat aatttacagg aaaggaatct
     1801 tttatgaaaa ttcaaaatac tgagtgggga tatattgaat ggaaacatac ttatgatgaa
     1861 aataatccaa aacaagctat gaatatatac attgcagtaa ctatgcctgg taagaaacat
     1921 tttaatcatg ttcattatgg tcaagagcag atgatatata ttttagaggg agagggtttg
     1981 tatattataa atggagtttg gaagccattt tatcaaggta tgatttttta tatagaatct
     2041 ggtagcacac atgaaactat aaatacaggt gatagagaaa taaaagaact tattgtttca
     2101 aataatgtag atgatgtagg agagagtgaa gttatagata ttaatcctaa taattattta
     2161 aagaagacct tgataaatta tagtgaaagt acattaaatt tgtatgcagc agtagaatca
     2221 attcgtggtc aatttataga cccgtttaaa attccattaa ttatatatga tgattcatgg
     2281 aatatagttt taaaaaatcc ttattttcca cttttttgtt ttgaaaaatg taatcctatg
     2341 aaatttcctc aaaattgtga ttgtatgaat caaaaaagta gcaatcaatt tgtatgtgaa
     2401 tatgggataa ctatatataa tataccaata ctgtacaaaa gcaatagcat aggtgttata
     2461 agaggagggt atgtactatt atctgatttg aatttagata cagagcataa taatttatat
     2521 gatattccag agggagctgc tagaagtata aaacgcctct tgaaacaaat atctaaaaat
     2581 ataataaatt tttgctcttt taatgatata agaaaggatt tacaagaaaa agagaagact
     2641 atagcaagaa catatcatta tggagagcaa cttgaaatga atttaaaagt agctcaagat
     2701 atggttacta atttaagaat aaatcatcac tttttattta atacattaaa ttcaatggca
     2761 agcatagcac tagatgatgg tagttatgat ttgtatagtg caataataga tttatcaaga
     2821 atgtttagat atacaatgag gtctgattta agatttgtag aattagaatc tgaaatatta
     2881 tacattaaaa attatttgaa tttacagaaa ttacgttatg gagatgcact taaagtaaaa
     2941 tatcttattc cagagaagtt atataacctt tctgtgcctt ttaactttat tcaacctatt
     3001 gtagagaatg catttacaca tggatttaga gatattgaca cagaaaagcg aattgaaata
     3061 attgcaagat tagattctca gtatgctatt atagagattc ataataatgg aactatctta
     3121 gatggaaata atatagacaa aataaaggca ggtattcgta gtaataatgg tcatggtttg
     3181 tctttaatat atacaaaatt cacatctgct tatggaaata attttgatat ggatataaag
     3241 tcgagtgata atgaaggaac ttacataatg attaaaattc ctattgaaaa ttataaggag
     3301 tatgtataga acttgtgaaa aattaataag ttttataaat gttaagaaat atattagatg
     3361 tttttatgtt gttttatata tcttactttt ttatattatt aagctaaaag ggaggtattc
     3421 tcatgagaag agcactaata atagatgatg aagaagctgt tctaaaaata ataacacatt
     3481 ttatagaaat gaaagatatg ccaataaaaa ttgttggtaa agcaacatct ggtgatgaag
     3541 cagtagataa aatcattggt ttagagccag acatagtttt tatagatatt caaatgccaa
     3601 tatacaacgg gttagaagta atagaaaaaa catctcattt atctaaaaaa ataaactttg
     3661 tagtaataac tgcatttaac tattttgaat atgctcaaaa agctttgaga ttaaatgtaa
     3721 aagatatact ccttaaacca ttggatatga aagaatttac taagtctgta gaaaaaatta
     3781 ttggatatca atataccaat aatgatttat taaatgagat actggaatat ataaatctaa
     3841 actatagcaa tgacttaaag ttaaatgatt gtgctagatt atttttaacc aatccatcta
     3901 atatatctag gatttttaaa agtaatttaa atacaacttt tatatcatat ctaaatcata
     3961 taagaataga aaaatctatt gagttacttg aaaatactac aaaaagtata aatgagattg
     4021 cagaaatagt tggatataat agcctaaata acttttataa aaactttaag atagaaaaag
     4081 gaatgactcc aaaagtttat aaactgaaca ctaaaaattg aaattgataa ataaaaagtc
     4141 aatatagata attgtttgag tgtgtctgaa taagtatact ttaattatga taaaaattaa
     4201 agcgctttaa acatatttaa taatattaaa ccttataaaa gctgtaccta tttatataaa
     4261 aaaatatata taaataaaaa gaaaaagata tacaaaataa aaaaagtgaa ttaaatactg
     4321 tttcaaaaag acagaatttt catttaattt actgagagtt tatatttctt aaaggaaggt
     4381 aactttataa tggaggtaat ttaatatgtc agacaaagtg aatatgaaaa atattatctg
     4441 tattgcaggc gcttatctag caacagcaat tggttctggg tttgctactg gtcaagaaat
     4501 tctacaattt tttgcctcac aagggtatat gggtttaatt ggagcattaa tatcaatggt
     4561 attgttttca tttcttggtg ctgaagttat aactagaggt agagaattaa aacttaaaga
     4621 accaacaaga atttacactt tttattgtgg gaagatttta ggaaaatttt acgaatggtt
     4681 tagtccaata tttttatttg ggattcttat agtaatgatt tcaggtgcag gctcaacttt
     4741 aactgaatac tatggtataa atccttactt aggaagagta ttgatggctg ttgctacttt
     4801 aattacagtg tctatgggtt tagaaaaatt agtaagcata cttggacgta ttggaccagt
     4861 gatagcagta tttacaattg ctgttggatt aataagcttg tttaagcatt ttgatggttt
     4921 atctatggtt ggagaagcta tgaaaaatat tgaagttaca aaagcatcag aaagtccaat
     4981 cgtttctggt gtaatatata atacattaaa tattattgta atgattgttt ttttaacagg
     5041 aatgggagct tctattgaaa gtaaaaagga tgctttttgg ggaggtttat taggtggaat
     5101 agtgtttatg actgctggta tagttatgta tttggctatg atttcagata taggaaattt
     5161 gtatatgaaa gaaataccat cactttattt agcagataat atatctccta ttgtaggtgt
     5221 atgtttttca gttgcattaa tattaggtat ctacacaaca gctgttccac ttttatggtc
     5281 tgtaaccaat agaattgttg aagatgaaca tcctaaattt aaattggtaa caacagtaat
     5341 agctattttg gcttgtatag gaggtttttt accgtttggt aaactagtaa acattttata
     5401 tccatataca ggttatatgg ggcttttaat tttagtctgt atggtgtata aagcaattac
     5461 atgtagaaaa aaatcaacac aaagtcagga atagagccac ttattaatag tttttattta
     5521 aagaaatttt ttatataatc cgaatttaaa aagtcaaaat agttaaataa attgtaaaat
     5581 atgataatat atttaattaa aataatatag taaaatataa atataaacta aaattaaatt
     5641 tataaaaaat atctttataa agattattaa cttatataat ttgtagttaa attataaaaa
     5701 ataaaaaaat aaaattttgg agaggtgtta gtatgcatga acttatttta atctcagaaa
     5761 agtataaaga agatatttta gattttttta cagatattca tatgcacccc gaattaagtt
     5821 ttaaagaatt taggactaca aaagctataa aagatttatt agtgagttta gacatagaaa
     5881 tattagattt aggaatggaa acaggagttg ttggattatt aaaagggaag tatgatggtc
     5941 ccacagtagc actaagagga gatattgatg cattgccaat atatgaagag gttgatatta
     6001 agtataaatc cagatatgat ggtataatgc atgcatgtgg gcatgatata cacacgtctt
     6061 gccttgtagg atgtgcatat gtattgtctc atattagaga tagtcttcat ggaaatgtaa
     6121 agtttatatt tcaaccagcc gaagaagtca ataaaggtgc gaagatgcta gtggaacgag
     6181 gagtaatgga aaatccaaaa gttgatgcta tatttggact tcataatcat ccagatatac
     6241 cttgtggaaa aataggtgtt aagttaggtg ggttaatggc agcagtagac actattaaga
     6301 tagaagtaaa tggatttgga ggtcacggag gtatacccaa cagaacaata gaccctatag
     6361 tagcatcaag tgcaattata atgggtattc aaaccatagt aagcagaaac ataagtccat
     6421 tagaatcagc tgttatatca ataggtacta taaatggagg gactgcaaat aatgtaattt
     6481 cagaaaaggt agatatgaca ggcacatgta gaagctttag taatgaagta cgaaagaaaa
     6541 tatcagaaaa tttagaaaat atagtttgtg agatagctcg tggatatcaa gcaactgcaa
     6601 agctagatta tttatttgat ttaccagctg ttataaatag taaagagatg tacgcaatag
     6661 cttgtaagtc tgtgtgtgat ttgtattcag aagatgctat agttgatcca ataccttcta
     6721 caggagggga agatttttct atattcatgg aaaaagcccc aggatttttt tactggttag
     6781 gggtaggcaa caaagaacaa gattgtatat atcagtggca taatcctaaa tttaaagctg
     6841 ataaaaattc tatattggta ggtacaaatg tactatgtca gtctgttata aattatatgg
     6901 ataaattaaa aaataaaatt taagatagag cattctggag ataaagttta tgagggggta
     6961 ttagtttgag gaaaattaat tcacaggaaa aaaacaaaga tagtggatta ttgatgaggg
     7021 cactaaatgt tgtagagaga gcaggaaata aacttccaga cccagtaacc atatttttat
     7081 tactatgtat aattgttgta atattgtcag cagttatatc taatttagga gtagaagaaa
     7141 tacatccttc tacaaaagaa gttgtaaagg tagtaaatct actagagaaa gagcagattc
     7201 aatcatattt aggtagcata gttacaaact ttcagtcatt tgctccttta ggattagtac
     7261 tggttacaat gcttggagca ggtgttgctg agaaatctgg atttatggaa gtgttaatga
     7321 aaaagggtat aagtaaagtt ccacaaaaac ttgtaactgt agcaatcgta tttgcaggta
     7381 tgttgtcaca tacagcagca gatgtaggat ttataatatt accaccactt gcagcacttg
     7441 tgtttttagg aattggaaga catcctcttg taggtatgtt tgcagctttt gcaggtgttg
     7501 cagggggatt tgcagcaaat gttatgctta gtactacaga tgtattgtta gctggattta
     7561 caataccagc agctcaaatg atggacccat cttaccaagg caatccagca atgaatcttt
     7621 attttgcttt tatatcagcg atagttttaa cttttgcagg agcatttgta actgaaaaat
     7681 ttatagctcc tcgttttaca aaatataatg attcaacaaa tgataaggat atacaggaat
     7741 tgagtgaact tgaaaataag ggtataaaat acgctttatt atcattgtta gttgtagtag
     7801 tagtgatagt tgccttatgt ataggtgaca atgcatttat gaaagatcca gagactggtt
     7861 ctatactatc tagtaatgca cctttaatga aaggtatagt tcctataatc actattatat
     7921 ttttaactcc aggattagtg tatggaaaag taagtaagaa gataaaaagt gacaaagatt
     7981 tagtatctat gatgggtagt agtatgagtg atatgggtgg atatatagtc ctagcattta
     8041 tagcatccca gtttattaat ttatttaatc ttagtaactt aggaacaatt ttatcaataa
     8101 caggagcaaa attactagca gagtcgggaa tacctagcta tggattgata ataggattta
     8161 tattgctatc tggatttatc aatttatttg taggaagtgc ttctgcaaaa tgggcgattt
     8221 tagcacctat atttgttccc atgtttatgc tactagactt taatccagca ttaactcaaa
     8281 tagcttatcg tattggagat gcttcaacaa atccaataag tccacttttt ccgtattttc
     8341 cagttatatt ggcctttgcc agaagatatg ataaagatat aggtataggt actgtcatat
     8401 cgaatatgat tccatactca gtggtattta cattaataga aataataata ttgttactat
     8461 tcatgggtat tggcatacca ttaggacctg gtggaggaat atcttacgtt ttatagaccg
     8521 ttataataaa tttatcaatg aagattaaac taataaaata tattctttaa ttaagaggag
     8581 gattattatg ttaaaaacaa caaaaatttc agacccagag ttatacaaga ttgtagctga
     8641 tgagttggtt agacaagaac acaatattga gatgatagct tctgaaagta cagcaccaac
     8701 agaagtactt gaattaagtg gatgtgtatt tacaaataaa acagaagaag gctatccagg
     8761 ggctcgtttt caagcaggtt ctgaagaagc agataaactt gaaaccctag caataaaacg
     8821 tgcaaaagaa gtatttggag cagaacatgt aaacgtacaa ccgtattcag gttctacagc
     8881 taactattgt gtatattcat caatattaaa accaaatgac actgttttaa gtatgcgtct
     8941 tgaccaaggt ggacacttga cacatggtag cgcagttaat tttttacatg acatttataa
     9001 atatgaattt tatggggtag acccaaatac tggacgtata gattatgatg ctcttgaagc
     9061 aaaggcaaaa gaatgtagac caaaacttat aattgcaggg gcaagttcat atccaagatt
     9121 aatagattat gaaagaatat caaaggttgc aaaagaagta ggagcttact ttatggtaga
     9181 tatggcacat gttgcaggat tagtcgcagc gaaagtaata cctagtcctg taccttatgc
     9241 agattttgtt tcttcatcta cgacaaagac tttctgtgga ccaagaagtg gtattgtact
     9301 ttgtaaagca gaacatgcaa agaaactcga taaaggtgtt tttccaggaa cattaggttc
     9361 tatacatctt aatacagttg cagcaaaagc cttttcacta ttatatttaa gtacagataa
     9421 atttaaaaag ataatggagc aagttgtagt aaatgctcag actctagctt ctgaactgat
     9481 ttcacatggt tttagtattg taagtggagg tactgataat catatagtaa tggttgactt
     9541 aagaagtaag aatcttacag gaaaacaatt tgaaaaagct cttgaatatg taggaataac
     9601 agttaataaa aatgttatac ctgacgaccc acaatcacca tttgtaacta gtggtgttcg
     9661 tataggtctt acatcaatat ctcaaagagg tctgaaagaa aaagaagtta tacagatagc
     9721 agggataatg aataaggtag cagaaaatat tgataataaa gaagtattag atgaatgtaa
     9781 agcagaggca caagaactta tatctaagtt tccattatat ccagaagggt attttgaaga
     9841 ttaaatattt tgatttgtat agctcacaat ttataaagcc tgtaacataa aatttaataa
     9901 tatataacca ctataaggtg ttgattaatc aacaccttat tttttttgaa aagtaatata
     9961 caagctatca taagcatata attccttata gataattaac aagggggata aagttatgag
    10021 gtattataat aaacctacca aagaagtatt aaaatactta aaaacaaatc cagaaattgg
    10081 acttgatgat aatgaggtag aagaaaggaa gcttagatat ggattaaatg aatttactat
    10141 aaaagaaggt agaacatttt gggacgagtt gggagaaagt ttgacagaac ctatgatatt
    10201 aattctcata ggagcagcag taataagttc ttttgtggga gaattacatg acgcattagg
    10261 tattttaggg gctattttta ttggaatttc tataggcata ataactgaag gaaaatctaa
    10321 aaaagcagct catgccctgt caaaattaac agaaaatata gaagtaaagg tgcttagaaa
    10381 tgggaagata attaaaatat caaaaaatga tttagtacca ggagatattg tatatataga
    10441 aactggggat atgataccag cagatggaag acttatacag tctataaatt tgaaacttag
    10501 agaagatatg ctaacaggag aatctgatga tgtagctaag aatgctgatg ctgtattaga
    10561 tatggaagta gtttattcta aaacagaaat aatcgaacag gatgcaatac ctgcaaagca
    10621 agtcaatatg gtatttggag ggactcttgt agcatatggt aggggcataa tggttgtcac
    10681 tcatactggt gataagacag agatgggtaa gatagcacag aatttatcaa atgaagacca
    10741 gcaaactcca cttcaaatta aattagggaa gttgggtgct aaaatagcag gaatatcagg
    10801 cataatagct actttattgt gtatgtttat gattatacag atgcagagaa aaggtatgtt
    10861 aattttagat acaagcagta tattatcttt cttacaatca ttagaacctg ctaaaaatgc
    10921 atatatggtt tgtattgcat taatagttgc aactgttcca gaaggtttgc caactatgat
    10981 aaatattacc ttagctataa caatgcaaaa gatggcaaag ataaatgcat tagtaacaaa
    11041 gaaagaagcc tgtgaaacta taggttctgt ttctgtaata tgttccgaca aaacagggac
    11101 tttaacacaa aacaaaatga tggtagaggt agcatatgtt gatggtaaat atataagtgg
    11161 aggagagtat caaagtaata gctattttga acaaaactgt atagtaaatt caactgcaga
    11221 tatagaaaaa gaagataatt catttaaata tataggaagc gcaacagagt gtgcattact
    11281 tttatatcat aatgataaga actataatga aatgagaaaa caaacgtatt tgatatcaca
    11341 aataccattt agttcagaag aaaagaagat gtcaacatta attcgtcaag aggattcaga
    11401 tatattactt tcaaaaggtg ctccagaagt attattaaaa aaatgttctt atgtacaaca
    11461 aggaaaaaat attgttccaa taactccaaa agtagaaaaa agtattttag atgagattaa
    11521 gaagttacaa attaaatcta tgagaacatt aggatttgct tataaaaaaa tgagtaattc
    11581 taaaacagaa gttgcaatga cttcagaagg tgaattgaat ttaataggaa attcaagaag
    11641 ttatatgaag gaagataatt tagtttttag tggatttgta ggaatagtgg acccattgag
    11701 agaaggggtc aaagattcca ttgataaagc atttaatgca ggagtagatg taaaaatgct
    11761 tacaggagat aatataaata cagcaacagc tattggaaat gaattaggat tattaaatga
    11821 tgggaaaaag gcagttgaag caacatatat agatgtattg acagataaag aattgagaga
    11881 agaaatcaaa ggaatatcta ttgttgcaag aagtaaacca gatacaaaaa tgagaatagt
    11941 ttcagcactt caaaaaagtg gcgaagttgt agctgtaaca ggtgatggta taaatgatgc
    12001 cccagcactt agtcaagcag atgttggaat tgcaatgggt atttcaggaa cagaagtatc
    12061 taaaaatgca gcagatataa tattgactga tgatagcttt agtactatag tagagggaat
    12121 aaaatgggga agaggtatat atgagaactt tcaaagattt atacagtttc agcttactgt
    12181 aaatatagta gcatttataa ttgctataat atctcaactt actggtaagg atatgccatt
    12241 tactacaata caattattat gggtaaatat aataatggat ggtcctccag ccctagcatt
    12301 aggattagaa cctgttagag attatgtatt gaaaagaaaa ccaataaata gacattctgg
    12361 aataatagct aggtctatgt ttgtaaatat tattataaat gcaattctta taattacaat
    12421 tgtatttact caatcagctt ttaatatatt gggagctaca tcagaagaac aaggtactgt
    12481 tatattttct ttatttgcat tcagtgccct ttttaatgca ttaaattgta gagagtttgg
    12541 tttaaatagt acaataccaa atttctttaa aaataaactg gcattgcaaa taatagtagt
    12601 tacagggata attcaaataa tatttacaca agtgttccag agtttcttta attctgtttc
    12661 tttggacttt gatatgtgga taaagataat actatttgca tccactatcc tattgtcaaa
    12721 tgaatttgta aagctaattc ttagaacaat gaaaaatagt agaactatga actttaatag
    12781 taaaaattaa ttcttatgaa accgtaaagt actatatatt gtaactttgc ggtttttgtt
    12841 tgtttaatgt tgttttatat taccattttc atatttaatt ttttattaat ctgaacacaa
    12901 tcttccattg taattgcttc atctgtgtca acacattcaa agtcaatatc taatttttgt
    12961 aatattttat atgcctcttt ttgcaaagga gtatcaaatt taataggtga ttttgtgtaa
    13021 atatcactga taaaaaacat gatttatttc attcctttcc atttaaaatc ttatagttat
    13081 agtgtatcat aaggttataa aatatagata aatatagtat aaatttaaaa tatgtacaga
    13141 caaaacaaaa aaatcatatt ctaagtaata cttgggaaaa atgatattat cataataaat
    13201 aatctttgta aaaacttata tagaatctat taaacagaca ctaagccata aaaactttac
    13261 taaatataaa attcgtatct aaatagatat atagaataat attttactga agtagagtga
    13321 tttttaagaa ttttatatat aaattatgtt atcataaata aagatataga ttataaaatt
    13381 tgttaataaa ataaagggca gggaggtaag tttcatgttt tccaaaatcc tagtcaaaac
    13441 atttatcaga gatagcgaaa acgttcaaaa tacagatgtt agaaataagt atggttatgt
    13501 agctggtgta gtaggtatat tatccaattt acttttattt gtgataaagg tattcatagg
    13561 aatgctcaca tctagtatag ctataatggc tgatgcattt aataatcttt ctgatatggc
    13621 atcttcagca ataactatga taggttttaa attagcaagt aagccagctg ataaagaaca
    13681 cccttttggt catggaagaa tagagtattt atcagcatta atagtagcat ttatggtgat
    13741 gttggttgga cttcaatttg taaagtcatc tatagaaagg atagtaaatc caattcctgt
    13801 aaaatttgaa gtaataccac ttatactttt aattgcatct ataatgatta aaatctggtt
    13861 gagtcgtttt aataaattta tgggaaataa aattgattct tcagccttaa aagcagtttc
    13921 acttgatgct ttaggagatg tatttacttc aagttgtgta gttatatcat ttattgtagc
    13981 tagatttact aattttccca tagatggata tgttggtatc gtagtatcat tagtgatact
    14041 ttatgctggt ttctctcttg taaaagatac tataaatcct cttttagggg aagcaccgga
    14101 tgaagaaatg gtaaattcta ttatagagct attgttatct tataaatata taataggtac
    14161 acatgattta attattcata actatggagt tggtagatgt attgcatcta tacatgcaga
    14221 aattccatcc aacatagata taatggaaat acatgaaatt atagatactg cagagagaga
    14281 aatatctgaa aaattagata tatatctagt aattcacatg gacccaatat gtcttgaaga
    14341 taaagaagtt atgtcagcca aaaaagaact tgaagaaata cttaagaaaa atagtcttgt
    14401 aaaatctatg catgatttta gaatagttgg aaaaggaagc aagaagaatt taatatttga
    14461 tatagtagtt aatccttccg aattttccaa aaacatgtca gaagatgact taaaagaaga
    14521 tataacaaaa ttagttaagg agataaatcc tgaatataat tgtgtaattg ttgtagataa
    14581 ggattttatt taattattaa ttaatctcat cataaggtaa aaaatgtaaa aaggatagac
    14641 aatagtatag aggcataata taatagaagt atataattgt atttttaata taaataaagc
    14701 aattttaatt gtttttatga aatattatag agatgttgaa gtatattcta ttattgggca
    14761 ccttatggat atactgagtc agtggtgcaa ccggctatga atataaattt atttattttc
    14821 atagcttttt ttgtataatt ttttagtaaa aattacttta tatacgatat taatggaaga
    14881 ttagattata aattataggt aacctaaagg ggtgagattt acatgaagaa aggaaataga
    14941 aaggcattat taattagttt aattatgatt ttaagtatgg tggtgtctac aatatatcca
    15001 actgtatctt atgcttcgga attaggagag aatagtcaga ttcaaagtgg ttcaactaat
    15061 tcatcaactg gagaggagaa ggaaagcgac aataaaaagc cagaacagac tccagagaaa
    15121 gataaggcaa cagataataa aaaaccagaa cagaccccag aggaagaaaa accaacagat
    15181 aataaaaaac cagaacagac cccagaagaa gataagtcaa cagataataa aaagccagag
    15241 caaactccag aaggagaaaa gccaatagat aataaaaagc cagagcagat accagaagaa
    15301 gataagtcaa cagataataa aaagtcagaa caagctttag aagatgaaaa accactagac
    15361 aataaaaata cagaaaaaac tccagaggaa gataatctat tagaaaatga aaatttattg
    15421 aaagttttag aagaagaact taatgaagaa aatgaagact atggatttgt tgtaaaaatt
    15481 aataacaata caatagaaac ggaatcaatg aaaaaaatat catttaattt aacttatact
    15541 ccaacatcaa aaggaataca agcaggagat tcaataactt ttaaggtgcc agatgtattc
    15601 aataaagtta atttagatta tacaagtgaa tgctttgata aaacagaaag taatggagag
    15661 tatacactta catttagaga attacctaat ggccaaagtg taatgcaagg taaaataggt
    15721 ttggaagcat atgttaaaaa ggttgatgaa gatactaatg caaaaataca tattgaaact
    15781 actggaaaaa tagaaagtgg aagtggagat atagatgtag aaataaaacc tggagataaa
    15841 acggatgttc cagatgctaa aggaacactt aaaaagcttg ttgaaggaaa aaaatcaact
    15901 actgtattta tgccagttaa aaataaagat ataaattaca gtatacaagt aaatgaaaag
    15961 caggaagagt taaaagatat tattttgtat gatgagcttc ctgaaggatt gacattgata
    16021 aatggttctg taagtgtagt aacaagtgat ggaaaagaag ttagtgattt taatattgaa
    16081 cagagtaaaa attctatttc agttaatctt ggaaatatag ataaatcgta tactgttaag
    16141 tataaggcta gaatcagtga caaaaatgca aaacatggta ataaatataa gaatgtagct
    16201 aggattgaat ctgatggtaa gaagatacaa gaagatgatg ctactgtaag tatatttgat
    16261 agaggagatg attatcttct tactaaagga cattcaggag caacaaatat tactcaagtt
    16321 ggtcaagtaa taaactatca gatttcaata aatgatgata aatcaccaat aagtaatgtt
    16381 gttataacag ataatatacc agaaggcatg agattaacta cttcaggaga agctgggcat
    16441 gattttagag ttgtagaaat tccaatgaat gggagctgga ctccttggag taaagaaaaa
    16501 attgccaata atatttcata taaagtagaa gaaaaaagaa atgaaagtgg gcaagtagat
    16561 aaagttataa ctggatttac aattaatctc agtaaagaag aagtggaatc aaagtttttt
    16621 atagcttata cattaaaggt aattagtatt gaagatagct atataaatag agctgttctt
    16681 gatgcaaata atagtgaaat tgataaaaat gatgagataa actttaagaa aaattctgga
    16741 ttaataagcg caaaaaaaga agttgataaa aaagttttaa atagtagtga taaccagatt
    16801 gttaaataca aaatcaatat gtcaacttat ggggtttatg atgcaggaca agtaaatctt
    16861 ttagatgaag taaactcagc tctagaaata tctaatataa aatattctga taatcttgaa
    16921 cttaaaaaag aagctggaga tggaaaaaat acaataagat tagtaaataa atacgaattt
    16981 aaacagataa aagaagggga acctgtgcaa agttgggtta catttgatgc agacttcaca
    17041 aatgtaaaag ttggggagac tatacgaaat gtggctcaga ttaatggttc aagtcctcca
    17101 ggagtagaga ctactaagca aggttatgct tttgaagcta aaaaagtgga tgctttagat
    17161 aaaaatgtac tttcaggtgc taagttcaat ttagaagatg catttggaaa tatagtagtt
    17221 aaggatttag ttagtgatga agatggtata attcaatcta gtataaaaaa accaggaaca
    17281 tattatttag tggagataat ggctccaagt ggatatgaaa aactgaagga taaaattaag
    17341 gttgaaattg gtaataaaga tattggtaga atagtggata ttggaaatat agaaaacata
    17401 agacaagaaa atccaccagt aaatccacca gcaccaccaa atacagatga accaatagta
    17461 aatccaccag taccaccaag cacagataaa ccaaggaaac catcaagttc atctgataca
    17521 gaggatacta tagttataaa tccaccaata ccaccatcag aagatataat aaatccgcca
    17581 ataccagagg tattgaatcc gccagtacca ccatcagaag agatgataga aactcctgtt
    17641 aaacagatta ttccaatacc agaagtagta aagccatcag tgtcagaaga aaaaaataat
    17701 aaagttaaag atgatacttt ggtaaatcca ccagtaccac caaaaacagg ggatagtact
    17761 acaattattg gagaaatact tttggttata ggagctattg taggacttat cgtattaaga
    17821 agaaataaaa atacaaatta gaccctaaca ttataaaaaa tataagttat attaaaaaat
    17881 atcttctaaa acaatattta ataatgaaaa tattgttaga agatattttt ttttgaagat
    17941 ttatgtaaaa aaataataac tttttagaaa aaagttccaa agggatatag acaattaaaa
    18001 agatataagc tataatatag atataaaatt aaatgaggca aaactaggga aacctagtca
    18061 cgcaaagcta taggggctaa agtttagtaa accatgccag ccagttgccg aaaagagtaa
    18121 aaatttcttt ttgtatatac aaagatactc ttgggtatct tttttatttt gaaatttatt
    18181 actcccggcc taactcaaag gagggaataa tatgagacca agtaaaaaat tattaatagc
    18241 tataatatca atattcttaa taagttcagt tccagtaagt gcacatgcag atagtactac
    18301 tatacaacaa aataaagaca cacttagtca aatagtagtt tttccaactg gaaattacga
    18361 taaaaacgaa gctaatgcta tggtaaatag attagctaat atagatggaa agtatttaaa
    18421 tgcattaaag caaaataact taaaaataaa attattaagt ggaaagttaa cagatgaaaa
    18481 agaatatgct tatttaaaag gtgtagttcc taagggatgg gaaggtactg gaaaaacttg
    18541 ggatgatgtt ccaggtttag gtggaagtac agtagctctt agaataggat ttagtaacaa
    18601 aggtaaaggg catgatgcaa taaatttaga attacatgaa acagcacatg caatagacca
    18661 tatagtatta aatgatatat ctaaatcagc acaattcaaa caaatatttg ctaaagaagg
    18721 tcgttcttta ggtaatgtaa actacttagg tgtatatcca gaagaatttt ttgcagaatc
    18781 atttgcatat tactatttaa atcaagatac aaatagtaag ttaaaatcag cttgtccaca
    18841 aacttatagc tttttgcaaa atttagctaa atagagtttt aataaattta aaataaatat
    18901 aaataaagag atgctattat aatagcatct cttttatatt gcgtttaaat ctaataaatt
    18961 ttgtcttatg tataactatg atagattatc aattactact ccttatacaa ggcttcaacg
    19021 tgtttattat gcatttgagt aattatcgta ggtataatag aaattattga accaattact
    19081 atcaataaaa ttcctataac catgtttata gggataattt ctttaagtat taataatgct
    19141 agtattggag atattactgg tttaaagaag aatactaaag atgtaagact agtagatgta
    19201 gcttccattg ccataaagta aagcgcataa ccaacacctg taacaaatac gaatacataa
    19261 atcataatgg gcatattgtg taaggtatat ccactaaaaa tagggatatt tgcaaataca
    19321 tgaagcttat ttttatttaa tatatctgcg ataaaattaa ttttagtgag taaaaccaat
    19381 atcaacattt ccaaactacc aaatataaag ctaaagcagg ttacaactat tccaccaaat
    19441 tttaaggttt ttcttttacc aaatacagta tacattgaaa aaataatagc agaagataat
    19501 gttaagaata ttccactaag agttagtttt gtatgcaatg gatttattat aactataatt
    19561 ccaagaactt ctaatataag tgaaattaca ttgtgcctat atattttttc cttcaaaatt
    19621 aagtaagcta aaagcattac aaagactgga ttacaactga acaaaacagc aactacagat
    19681 gcctttgtgt ttagaacagc aagttgatac aatgacatac ttactactac acacataaaa
    19741 cctaaaaatg caaagtattt taagtctgat tttgtaatag aaagactaag agctcttaat
    19801 ctttttacag caaaaggtag taagactaaa ccgccaacaa aaaatcttga taaagttatt
    19861 tgtaagggat taaagtcatt agtgacagtt tttagactaa tttccattga actaaaaaat
    19921 aaggtagtca gtattatata aacataacct tttttcaagt caaacacctc cttgtaattt
    19981 atattagtac tatagataag aatattcaag catattggaa taatatggta tgtataaaat
    20041 atagtacata aaattataaa ctaccttaat atagaatgta agtattctat actaaggtag
    20101 ttaattattt gaattacttt gatttagtta ttttaaatta tataatttgg ctaatttttc
    20161 aaatgctggt aaagaacttt taactttatc ataagataca caatatgata ctctaaaatg
    20221 acctggacaa ccaaaagaag aacctggtac taataataaa ttaaattgtt ttgcatcatt
    20281 gcagaatttt ttatcatctt caattggagc ttttgggaac aaatagaatg taccattagg
    20341 tttaacacaa gaatatccca tatcgattaa actattatat agaaggtcaa aattcttttt
    20401 ataaatattt acatcaactt ctgcatctaa acttcttgca ataacccttt gaaaaagaga
    20461 aggagcattt acaaatccaa gtattctatt tgcaacattt aatgaagaca taacatcatc
    20521 aaaatcatcc atttgcccat tagcaacaat atatcctata cgttcaccag gtagagataa
    20581 tgatttacta taagagtatc ctataaatga atttctatag tattttagta cacaaggaac
    20641 tttggcacca tcataaatta tttctctata tggctcatca gaaattaggt atatgcttgt
    20701 attaaattct ttttgttttg aatccaataa ttctccaaga ttttttaata attcttcaga
    20761 atatatgaca ccagtaggat tatttggagt gtttattatt aaagctcttg tttttggagt
    20821 aatagcattt tttagagcat ctaaatctgg ttcaaaagtc tctatatttg taggaacttc
    20881 tattaatttt ccatcatagt tttctgtata atttttatac tcaccaaaat acggtgcaaa
    20941 agcaataact tcatcaccag gatttaaaag agtttttaaa ataatattta gaccaccagc
    21001 agcaccacaa gtcataatta aattttctct tgtaagattt aatccatctt ttttatttat
    21061 gtgttcagca atagcatctc ttacatcctc atatcctgag ttgttcatat atccatgaac
    21121 taaatttgga gattcttcat ttaaaatatc atttataact gctttaatag tctctggagg
    21181 ttcaacactt ggatttccta tactaaaatc aaaaacattt tcctcaccat aaatatcaga
    21241 aagtttcttt ccttcttcaa acatagctct tataacagaa ctatttgcaa ctaaagtttg
    21301 cattttatta gatatcatat gtaacactcc tttttataga tttatattgc ttatgtatat
    21361 attaccatat ttttactgaa tttagacaat tttcgaaaaa ttacttgttt gaaaatataa
    21421 a
//
LOCUS       DAMJZE010000050        21377 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJZE010000050 DAMJZE010000000
VERSION     DAMJZE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.050"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            182..1057
                     /locus_tag="PC541_001668"
     CDS             182..1057
                     /locus_tag="PC541_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF2839135.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1075..2565
                     /gene="cls"
                     /locus_tag="PC541_001669"
     CDS             1075..2565
                     /gene="cls"
                     /locus_tag="PC541_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2839136.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2758..3042
                     /locus_tag="PC541_001670"
     CDS             2758..3042
                     /locus_tag="PC541_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2839137.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3075..4703
                     /gene="groL"
                     /locus_tag="PC541_001671"
     CDS             3075..4703
                     /gene="groL"
                     /locus_tag="PC541_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2839138.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4965..5705
                     /gene="larB"
                     /locus_tag="PC541_001672"
     CDS             4965..5705
                     /gene="larB"
                     /locus_tag="PC541_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2839139.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5789..6523
                     /gene="larC"
                     /locus_tag="PC541_001673"
     CDS             5789..6523
                     /gene="larC"
                     /locus_tag="PC541_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2839140.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6553..6972
                     /locus_tag="PC541_001674"
     CDS             6553..6972
                     /locus_tag="PC541_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2839141.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCSES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7032..7133
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7354..8889
                     /gene="guaA"
                     /locus_tag="PC541_001675"
     CDS             7354..8889
                     /gene="guaA"
                     /locus_tag="PC541_001675"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2839142.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(8966..10012)
                     /locus_tag="PC541_001676"
     CDS             complement(8966..10012)
                     /locus_tag="PC541_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2839143.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(10089..10304)
                     /locus_tag="PC541_001677"
     CDS             complement(10089..10304)
                     /locus_tag="PC541_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2839144.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(10738..11139)
                     /locus_tag="PC541_001678"
     CDS             complement(10738..11139)
                     /locus_tag="PC541_001678"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839145.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            11304..11516
                     /locus_tag="PC541_001679"
     CDS             11304..11516
                     /locus_tag="PC541_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839146.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
     gene            11756..11893
                     /locus_tag="PC541_001680"
     CDS             11756..11893
                     /locus_tag="PC541_001680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839147.1"
                     /translation="MQREQTTLRIPEDLHKALIDLSSDIGMPITSIIIIACWLYISKI
                     N"
     gene            complement(11879..12190)
                     /locus_tag="PC541_001681"
     CDS             complement(11879..12190)
                     /locus_tag="PC541_001681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839148.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSINSLINNVVHSFCISDWLIY
                     L"
     gene            12311..12481
                     /locus_tag="PC541_001682"
     CDS             12311..12481
                     /locus_tag="PC541_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839149.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            12484..13122
                     /locus_tag="PC541_001683"
     CDS             12484..13122
                     /locus_tag="PC541_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839150.1"
                     /translation="MNNLQIFEKLEFGQIRMAEIDNKPYFVGKDVAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPSIRET
                     GQYQAQQHVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIRVIKSERPYKQLSYFEN"
     gene            13181..13351
                     /locus_tag="PC541_001684"
     CDS             13181..13351
                     /locus_tag="PC541_001684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839151.1"
                     /translation="MKNNISDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSTPAK"
     gene            complement(13354..13587)
                     /locus_tag="PC541_001685"
     CDS             complement(13354..13587)
                     /locus_tag="PC541_001685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839152.1"
                     /translation="MNIGKITKIEATCFDCGTKVTITESKFREVCDNGLPCPMCKEHI
                     SNSNFIAEQALQYNHAVNKLEKELDSCKNIHIY"
     gene            13656..13853
                     /locus_tag="PC541_001686"
     CDS             13656..13853
                     /locus_tag="PC541_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839153.1"
                     /translation="MAINDNINKILRDRNLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            13892..14098
                     /locus_tag="PC541_001687"
     CDS             13892..14098
                     /locus_tag="PC541_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839154.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            14270..14458
                     /locus_tag="PC541_001688"
     CDS             14270..14458
                     /locus_tag="PC541_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839155.1"
                     /translation="MRIIYKNKIYKVEQDKVLFRITYYDEQKNNRKFNNNKKVKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            14468..14686
                     /locus_tag="PC541_001689"
     CDS             14468..14686
                     /locus_tag="PC541_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839156.1"
                     /translation="MHMVFNLEKFKVGNAIRISCERFGFEIDCIVVVATEEELNLAYF
                     DKERGCIEYQALMPEDLRYDDYILERLG"
     gene            14702..14872
                     /locus_tag="PC541_001690"
     CDS             14702..14872
                     /locus_tag="PC541_001690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839157.1"
                     /translation="METAISIAKGQIEGANESIKELKSKENYDKESLRWWEGVKQASE
                     NILEFLEIENKA"
     gene            14954..16906
                     /locus_tag="PC541_001691"
     CDS             14954..16906
                     /locus_tag="PC541_001691"
                     /inference="COORDINATES: protein motif:HMM:NF022840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HDF2839158.1"
                     /translation="MKAILLKSLNIENFKGIKELRIDFNNITNVFGENATGKTSIFDA
                     FTWVMFDKDSKNRSVFEIKPLDKQNKVIRGLVTTVTAVLEVNNKEIKLTKKYEEKWTR
                     KRGESEATFTKNETTYMINDTPIKKSEYVKEVAEIANEEQFKLLTNPYFFSNELNWKK
                     GRELILNVCGDITTEQIIESNKDLTLLITEFEKENNIDKIIKNRKAIKNNLSKEKEEI
                     PVRVNECNKSMYDIDFEEIEAQLKAKKADLEAIEDMLLSGTKANEQILKDKEKIFELK
                     QETQSIKQSASEKGNKKRNELLKEKDELKYNIKNLRNNLVYLERDNELKETLKKRAIE
                     KTTNLRDKWTKKSQETLDLSVIQTECPTCKRPLDLEDIEEKKKEMLDNFNLNKAKELK
                     EIAELGKSKNDDVELFNTEIERLKVDINKTLEEIQEKAVLLDKIKKELESTKSTEIYS
                     ADEEKRLTEISAEIKELEEKINNKDADKNIDELKENKKKLTSEIELLNKELAKRDINK
                     ELLDRKEQLLEKEKALGIELAHQEKILNLCELFIKTKVSLLESNISSKFKNVTFKLFK
                     EQINGGIEETCEALINGVPFSNANTAGQINGGLDIINTLSNHFEVKMPIFVDNRESVN
                     NLIDIDSQVINLIVSNDNPLKIEGVN"
     gene            16907..17824
                     /locus_tag="PC541_001692"
     CDS             16907..17824
                     /locus_tag="PC541_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDF2839159.1"
                     /translation="MAERNITDVVLNRVNELKDSGELVIPPTYSAVNALKSAYLILQE
                     TLDKSYKPVLETCTPISIINSLLNMVIQGLSPAKKQCYFVAYGNKLQLMRSYMGTIAV
                     TKRLSNIKDIKAYCIYEGDKFKVKYNKENAVLEIGEYEPAFENIDIGKIKGAFAVIVG
                     NEGILHTEIMTFTQIERAWLQGVGYSNPKNKVHDNFTEEMAKKTVINRACKMYANTSD
                     DSDLLIGAFNETDRVITSEEIAKDTDKEVEKEISQNANIKSLEMPSQVDNTIFTQTKE
                     KVAINIDEDKPNLAEFEEAIQVELDTPPF"
     gene            17827..18537
                     /locus_tag="PC541_001693"
     CDS             17827..18537
                     /locus_tag="PC541_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2839160.1"
                     /translation="MIKILGSSSRGNCYLLQLENETLILECGVSYKEILKGLDFSLEN
                     VVGCLVTHEHKDHSKAITELTNNGIDVYSSKGTLEALKIENHRTKIIESEKLFKIGNF
                     KIVPFNTKHDAVEPLGFLINHSSFGNLLFITDSYYCEYNFNNLNHIMIECNYSKDLLD
                     NNKDKIYLRNRIVKSHFELSNVINFLKVNDLSNIKTITLLHLSEDNSDKDLFIREIEK
                     NVGVPVIVAEKGLEIYLD"
     gene            18695..19441
                     /locus_tag="PC541_001694"
     CDS             18695..19441
                     /locus_tag="PC541_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2839161.1"
                     /translation="MEMQTGYNRETVEKLIKRFIEYGKIKYDSTTKEILIINWSKHNF
                     SKSPKVRTCILKEVESIKNKEFQQYMYRVCIDYGYPIDTVSIDYGEKEKEKQKEKEKQ
                     KEKEKQKEKVEPLICEEQEKNELKEFKKLYEENIGVVYPVTAEWLIEISSDVDIRLFK
                     SAIEICAEKMNMNIAYLKGILKKWKDANITTYEQLESYRLQQENKKAKKVVNGQLSKN
                     KFANFEQTFTKYTENELDNIIKKSQDAKFK"
     gene            19499..19846
                     /locus_tag="PC541_001695"
     CDS             19499..19846
                     /locus_tag="PC541_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839162.1"
                     /translation="MGKIWMDAGTFLETKDIGDIECNLREMINKNKQKNLIGRINSKG
                     QGRKSKKVECTNIITGESKIFVSAVEASEYLYFTELHITRLARTGKTTKNGWKVRYIE
                     EVSNGISKCGTSN"
     gene            19815..19952
                     /locus_tag="PC541_001696"
     CDS             19815..19952
                     /locus_tag="PC541_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839163.1"
                     /translation="MVLANVEQVIKLAEKILSKKKCSVNKAIDIAIKILNRYEYEGMI
                     K"
     gene            19949..20398
                     /locus_tag="PC541_001697"
     CDS             19949..20398
                     /locus_tag="PC541_001697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839164.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNAVDCLLMLKEDFKWRKVC
                     NVGICSEEWAGNNQEIFEGDILKENYNPHNRSSYGIVKRDFNSIKLYLEWHYLKKFEG
                     EWIEIIDKTEIYHSRGYKVIGNEYENLEEVRKEFLERKEMLENERLS"
     gene            20500..20658
                     /locus_tag="PC541_001698"
     CDS             20500..20658
                     /locus_tag="PC541_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839165.1"
                     /translation="MNDRLEMINASVNYIQMICESSNIAIIAEHDRVRILDLETKEKY
                     DLLRSEGD"
     gene            20662..20985
                     /locus_tag="PC541_001699"
     CDS             20662..20985
                     /locus_tag="PC541_001699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839166.1"
                     /translation="MSKVIQCDFCKEIFEKYNGECIELYKKDCSKGVLGIDKHICPTC
                     YEKFIEGKIEKVENAKRFKDKLIDFLVEHEVCDCCACDCVFDSNDEKKCKAGIREWVE
                     SEVEE"
     gene            20987..21286
                     /locus_tag="PC541_001700"
     CDS             20987..21286
                     /locus_tag="PC541_001700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839167.1"
                     /translation="MAKLIVSIPLTKYLKDITKEWGETYITKTYGGHVYLSDEAPGEV
                     FKKEDDLNYIEFNDSDIVMRLPKEVHQHINLKKGCNKNLKDVLKEIKKIEEKTNI"
BASE COUNT         8756 a         2144 c         3950 g         6527 t
ORIGIN      
        1 tagaaattca ttttttattt aaggtaaaaa tttaaagtaa aatattattt tacataataa
       61 taaccttata aattttgaaa ttacttatct ttgtgttaaa ataagtaaaa tgcacatatt
      121 agagctaagt ttttaagtaa ataaacaaaa tgaaaatata aatattaagg agatttggta
      181 tatgaatgtt tatgaaaaag gtaatggagt tatactagaa ggagtaacag attttgaccc
      241 agtacatata tttgagtgtg gtcaatgttt tagatggcac aaacaagaag atggctcata
      301 cactggagtt gcaaagggaa gaatattaaa tgtcaaaaaa gagaatgata aaatatattt
      361 aaataatact aatttaaagg agtttaacag catttggtat aattactttg atttaggaac
      421 tgattataca gaaataaaga ataagctaaa aaatatggat gaatatttaa ataaggctac
      481 tgaatttgga tggggaataa gaatattaag acaagatgga tgggaaatgc ttatatcatt
      541 tataatttcc tctaataata ggattcctat gatacaaaga gcgatagaaa atttatcaag
      601 aaagtttggt aagtatatag gtgaatatga aggtaatgaa tactatgcat tcccaacacc
      661 agaagaacta aataaggcct cacaagagga aataagagca tgtcaaacag gttttagaga
      721 taagtacata aaaagtacta cacaggcagt tatagagaat aatgatgagg tatctgaata
      781 tacaaattta agtacagagg attgtagaaa agagctttta aaatttaatg gagtaggacc
      841 aaaagtatgt gattgtattg cattatttgg tatgcaaaaa tatgattctt ttccagttga
      901 tgtttgggtt aagagagtta tgcaagaatt ttatatagat gaagacatga gtttacctaa
      961 aatgagaact tatggaatcg ataaatttaa agaaatgtct ggatttgctc aacagtactt
     1021 attttactat gcaagagaac tcggtatagg aaaataaatt aagggggaga aattatggga
     1081 gtaataggaa caatatttct gttctattta attatatcat atctagctgg agccataatt
     1141 tctgtgatta tattgcttga aaatagagac cctgcaaaaa caatgtcttg gctattgatg
     1201 tttattatat ttccaggtgt aggtttaatg atatatgcta tttcaggtag aaatattaga
     1261 aaaagaaaat tatttaagac tcaaaaacta gctaataata taaaagaaaa gaaactattt
     1321 gatacattag aaaagataac agaaatagtt gaattagaaa aagaatctat aaaacaaaat
     1381 aagcttttaa gagatgaaga ggatggtagt tatagaaaaa gggtaataaa tatgttgtta
     1441 aagactggta tgtttccctt tactaaaaat aataaagtgg atgtatttgt agatggaaat
     1501 gaaaagttta aaagacttat agaagatata agagaagcta aagaccatat tcatttagag
     1561 tactttatca tcaaagatag tgaaataggt agggtgttaa aagaagaact tataaaaaag
     1621 gctaaagaag gcattaagat aagaatttta tatgatgatg taggatgctg gagattttgg
     1681 tttaatagaa aattttttcg agaaatgagg gaagtaggta tagaaattgc agcattttta
     1741 cctacaaaat ttcctataat aggagggaaa ttaaactata gaaatcacag aaaaattgtt
     1801 gttattgatg gaataattgg atatacaggt ggaataaata taggagatga atatttaggc
     1861 aaaaatgata agtttggata ttggagagat actcatatta gaattaaggg tatttctgta
     1921 tacatgcttc aaatgacatt tctgattgat tggtattata cgacaaaaga agtattagtg
     1981 actaaaaatt atttcccaag tgtagggaat gttggtgaaa gtatgataca agttgttgcg
     2041 agtggtccag acagtgactg ggaagatatt cattatgctt atttttctgc aatatgtcag
     2101 gctagaaaaa atgtttatat agaaactcca tattttattc cagatgaaag tctactaaaa
     2161 gctataaaga gtgctgctct tagtggagtt gatgtgagaa taatatttcc taaaattgca
     2221 gaccacaaga tagtaaatat agcatcttat tcatattttg aagaaatatt aagagcaggt
     2281 ggtaaagttt atttatataa taaaggattt atacattcta aagtcgtaat aatagatgat
     2341 aaaattgctt ctgcaggtac agctaatatg gatttaagaa gttttatgct taactttgag
     2401 gtaaatgcat ttatctatga tgaagaagtt attagggtaa tgacagatga tttctttgag
     2461 gatttaagtc actgtgaaga acttaatttg gaggtattta agaatagaaa tatcatacaa
     2521 aaaatcaaag aatctgtagc aagattattt tcaccaatat tataaaaaat tattaaaaaa
     2581 ggtattgaaa attatgtgtg attggtatat tataataatt gttagcactc tattagatag
     2641 agtgataata aataaaaagt atatatataa atctacatat ataagtagta ttaatttaaa
     2701 aataactaaa aacataggcg aactagtcta tacttaatat tttaggaggc gtgaataatg
     2761 aaaataagac cattagctga cagagtagta attaaaaaag tagaagcaga agaaaaaact
     2821 gcaagtggaa tagttttacc aggagcagct aaagagcaac ctcaaatagc tgaagttgta
     2881 gaagttggac caggtggaat agttgaaggg aaagaaataa aaatggaatt gacagtagga
     2941 gataaggtta tattccaaaa atattctgga acagaagtta agatagaagg acaagaatac
     3001 acaatactaa gacagagtga tgtattagct gttattgaat aaatatagaa taaatttatt
     3061 aggaggggtt taaaatggct aaagaaatta aattttcaga ggaaacaaga agagctttag
     3121 aagctggtgt aaataaatta gcagatacag taaaagtaac attaggacct aaaggaagaa
     3181 atgttatatt agataaaaaa tttggttctc cacttataac taatgatgga gtaactatag
     3241 caaaagagat agaactagaa gatagatttg aaaatatggg tgctcaatta gttaaagaag
     3301 tagcaactaa aactaatgat gtagctggag atggtactac aactgctaca gttttagcac
     3361 aagctataat aagagaaggt ttaaagaatg taacagcagg ggctaaccca atacttttaa
     3421 gaaaaggaat acaaaaagca gtaacagtag cagtagaaga attaaaaaat caatcaagaa
     3481 tagtagaaac acaagaggct atatctcaag ttgcttctat atctgctggt gatgaagaag
     3541 ttggaaaatt aatagcagaa gctatggaaa tagtaggtaa agatggagtt ataactgttg
     3601 aagagtctca aactatgaat actgaattag atgctgttga aggtatgcag tttgatagag
     3661 gatttgtttc tgcatatatg gttacagatg tagataaaat ggaagcagtt ttaaacgacc
     3721 catatatatt aattactgat aaaaaaatat ctaacataca agaattatta ccagttcttg
     3781 aacaaatagt tcaacaaggt aaaaagttat tgataatagc tgaagatgta gaaggtgaag
     3841 cattatctac attagtagta aataaattaa gaggaacatt tgatgtagta gcagttaaag
     3901 ctccaggctt tggagataga agaaaagaaa tgcttcaaga tatagcaata cttacaggtg
     3961 ctcaagtaat atcagaagaa ttaggttatg atttaaaaga agctgattta tctatgctag
     4021 gtagagcttc atctgttaag gtaactaaag aaagtactac aatagtagat ggttctggag
     4081 ataaaaaagc tatagaagat agagtaactc aaattaaaca tcaagtagag caaactactt
     4141 cagattttga tagagagaaa ttaatggaaa gattagctaa acttgctgga ggagtagctg
     4201 ttgtaaaagt tggagctgct acagaagtag agttaaaaga aagaaagtta agaatagaag
     4261 atgctcttaa tgcaactaga gcagctgtag aagaaggaat agtagctggt ggaggaactg
     4321 cttttgttag tgttatacca gcaataggaa cactaattga gagtttagaa ggagaagtta
     4381 aactaggtgc tcaaatagtt aaaaaagcgt tagaagagcc attaagacaa atagctataa
     4441 atgctggtct tgaaggtgct gtaattgtgc aaaatgttgt taattctgaa gcagaaactg
     4501 gatttgacgc attgaatgaa aaatatgtaa atatgataga agctggtata gttgacccaa
     4561 ctaaagttag tagaagtgct ttacaaaatg ctgcatcaat agcaagtact ttcttaacta
     4621 ctgaatctgc tgttgctgac cttcctgaaa aagaagatgc aggaatgcca ggaatgggtg
     4681 gcggaatgcc tggtatgatg taatttaaaa aatttaaaat ataaatttta atataaagaa
     4741 ctatttcgac ttagatttta aactctaagt tgaaatagtt cttttgtata tatctaattt
     4801 ttgtatatct ataaattcaa tacacatatt tagataatca gtatctagtt tgtaataata
     4861 ttttagagta gtattgttat tttttaggtt atgattttag atatatatta attaaggtat
     4921 aattgttgat agagaataaa tcaaatttaa aaggatgcga taatatggac ttaagaaaat
     4981 tgcttgaaga agttaaaaat gaaaatattg atatagattt tgcgttagaa aagttaaaag
     5041 atttacctta tgaagacctt ggatatgcaa atatagacca tcatagagaa ttaagaaatg
     5101 ggtatccgga agttatttat tgtgaaggta agagtgatga gcatatttta gggattattg
     5161 ataaaatgaa tcaaaaaagt tctaacatat tggggacaag gtgcagaaaa gaaacttttc
     5221 tgaaaattaa agaaatctat aatcatgctg agtacgaaga ggcgtctaaa attcttaaga
     5281 ttcaaaatca tgatatagag aatataggaa aaggtaaaat tgtgattgct acagggggaa
     5341 cttctgatat accagtagca gacgaagctt atcatacagc taagttttta ggtaatgatg
     5401 ttgatagaat atatgatgtt ggagtggcag gtatacatag gcttttaaat aaaagacata
     5461 aaatagatag tgctagagta atagtagccg ttgcaggtat ggaaggtgct cttgcaagtg
     5521 taattggtgg actggttgat gttccagtaa tagctgttcc aacttctgtt ggatatggag
     5581 caaattttgg aggattagca gcattgctag ctatgttaaa tagctgtgca tctggaatat
     5641 ctgttgtaaa tattgataat ggatttggag caggatattt agcatcaatg ataaataaac
     5701 tgtaaataca ttgatataaa ttaaacaaaa ctatataata aaatttaatt tctattatat
     5761 ttttactatt aaaattgagg agacatatat gagtgaaaaa atactttatt ttgatataat
     5821 aagcggtata tcaggggata tgacccttgc aagtttatta aatttaggtg tgcctaagga
     5881 aatttttttg gaggaaattc ataaactaag aatgagcgat gaatttaata taaacatcag
     5941 ctcgaaaact gaaaatggaa tagtaggtac aaaagtagaa gtaattacaa aagaaaaaca
     6001 tacacataga aatttagtgg atatatttga ggttatagat aaaagcactc taaacgaaaa
     6061 tgtaaaatct aaagcaaaaa aaatatttat ggtaattgga gaagcagaag caaaagttca
     6121 tggaactaca atagacaaga tacattttca tgaggtaggg gctatagatt caattgtaga
     6181 tatagtagga gcttctatat tagttgactt actttgtgta gataaagttt gtgctagtac
     6241 agtgtctgtt ggttcaggat ttgtaaaatg tgaacatgga attataccta tacctgcacc
     6301 tgcaactata gaaattttaa aaggtgtgcc aattaaatta aataatgtaa atggagaatg
     6361 tacaactcca acaggagctg caatcataaa agttttgtgt gatgagtttg tagatgagct
     6421 tgaattttct ccaaagcaaa ttggatatgg tataggtcat aagaagtttg aagtaccaaa
     6481 tatactcaga actgtattag gtgaaaaaaa aaagaagaat taatttatga aattagtgca
     6541 aacattgatg atatgtcttc agaaatctat tcttatttgt atgaaaaaat attagatgag
     6601 aaagcattgg atatttatac agagagcatt tatatgaaga agaatagacc agcaacaaaa
     6661 gtatcaattt tatgtagtga atcagattta gaaaaattta ttaaaatatt acttttagaa
     6721 actagtactt ttggagtgag atataatgca tataatagag aaattctaga gagaaaattt
     6781 gtaaaattag atacaaaata tggagtggtt acagtaaaac tagggtatta taaaggaaaa
     6841 ctagtcaagg ctactccaga atataatgaa tgtaaaacta tagcaaaaaa tgaagatatt
     6901 cctataaaaa aagtgtttgc tgatataaat tttagaataa aagaagaatt tagtgaaaac
     6961 ttattgacat aagtaataaa ttttgataaa ttaatattca gccttattaa ataaaagtaa
     7021 acagaaaaaa atatataaaa tataaactca ctcgtatatg ctcagaaata tggtctgaga
     7081 gtctctacca agataccgta aatatcttga ctatgagtga aattattata ccaaaagacc
     7141 tagttaagta aatgtgtagg ctgagttaaa ctccctatgt gttggctata agataaaggt
     7201 attttggaag cctgtatagt aatttcactt ttgctagaaa ttatggaagt tactatacag
     7261 gttttttgtg taaaaagatt taatataatt tagactgtat tgtgttaaaa taaatatata
     7321 aaaactatat tatacaagga gagatgtgaa tttatgaaac atgaattagt acttgtaata
     7381 gattttggag gacaatataa tcagttgata gctagaagag ttagagaaaa caacgtatat
     7441 tgtgaaatac ttccatgtac tgcaagcata gaaagaataa aagaaaaaaa tcctaaaggg
     7501 ataatattta caggcggtcc aaatagtgcg tatttagaag attctccaac aatatcaaaa
     7561 gaaatatttg aattaggagt accaatactt ggaatatgct atggtattca aataatgtct
     7621 catgtattgg gtggagttgt aagaaaaggt aataaacaag aaaaagaata tggaaaaaca
     7681 gctataactt atggaaaatc atcattattt gaaggaataa ctacaaatag tgtatggatg
     7741 agtcatacag atttaataga aaaagttcca gaaggattta ctatagttgc aaatactaat
     7801 gattgtccag tagcagctat ggaaaatgta gaacgaaacc tatatggagt tcaattccat
     7861 ccagaggtgg aacactgttt ggaaggggat aagatactta caaacttctt atataatatt
     7921 tgtaaagtaa aaggtgactg gactacagat tcattcatag aagataaaat aaaagaatta
     7981 aaagaaaaaa taggcgataa aaaagcttta tgtgctttaa gtggaggagt tgattcttct
     8041 gttgctgcag ttcttattca taaagcaatt ggggacaact taacttgtat atttgttgac
     8101 catggtttac ttagaaaaaa tgaaggaaat gatgtagaaa gaatatttag agagaaattt
     8161 gatataaatt taataagagt taatgcagaa gatagattct tatctaaatt aaaaggagtt
     8221 tctgaaccag aagctaagag aaaaataata ggtgaagaat ttataagagt atttgaagaa
     8281 gaatcaaata aattaggaaa aatggatttc ttagtacaag gaacaatcta tccagatgtt
     8341 atagaaagtg gacatggaaa tgctgctact ataaagtctc atcataatgt tggtggaatc
     8401 ccagaagatg tagacttcca agaaattgta gaaccattaa gagagttatt taaggatgaa
     8461 gttagaaaaa taggattaga gcttggtata gaagaaggat taatatttag acatcctttc
     8521 ccaggaccag gtctaggaat aagagttata ggtgatgtaa ctaaagaaaa atgtgatata
     8581 ttaagagaag ctgatgctgt ttatatggac gagcttagaa aagcaggttt atacagagaa
     8641 atatggcaag cttttgcaac attaccagat gttaagactg ttggagttat gggtgatgaa
     8701 agaacatatg cttatttagt tggtttaaga gcagttactt cttctgatgg aatgacttct
     8761 gactggtata agatgcctta tgatgtatta gaaagaattt ctaatagaat aataaatgaa
     8821 gttgatggag taaatagagt agtttatgat ataacttcta aacctcctgg aactattgaa
     8881 tgggaatagt aaataaaaaa gtcgtaagcc ttgtattttc aaggtttgcg acttttaaaa
     8941 aaatctttgt gatactaaaa tgatactata tatttaaatt atctagtttt tgggcaactt
     9001 caagttgctt atttgggtat aaatgtgaat atgtattcca agttgtttct actttttcat
     9061 gccccaatct ttctgctata gttaatatat ttacatccat atttaccaat aatgacgcat
     9121 gagaatgtct caaatcatgc actctaattc tttttacatt agataactta cagcatctat
     9181 ctagctcttt agaaagataa cttttagcaa atttaaatat tctttcatta tcttttaagt
     9241 catatagctt agatagataa tcttttatat tattatataa gaaatgtggg atagggacaa
     9301 cacgtttact tttaggagtt ttaggagaag aaacaatatc ctctccattt agtcttatgt
     9361 agcttttttc gacgcttatt ttattttcga aaatatcttt tggagtaaga gctaacaact
     9421 cccctagtct aagacctgtc caaaataaaa tttcaaatgc tagtctaggt tctgatttct
     9481 tttcaaattc aataaatttt ttaaactctt ctaatgtcca aaagttcatt tcatctgcgt
     9541 tttttttacc gatagaacct gccaaatgag caggattaga aggtaagtta taatatttaa
     9601 ctgcataatt taatacagca acaagctggt tgtttatcgt tttaatatat gtttggctat
     9661 aatcactttt taataattcg ttttgccatc ttcttatatg tgttgcagta atttcattta
     9721 tttttagact tttaaaaaaa ggtaaaattc taaagtttat taaatacttt ttagtttcta
     9781 atgtagataa ttttaatcta gaagacatat cgtgcatgta ttcttctatg agactttcaa
     9841 aactcatatc agtactcatt ttagatttat tcaaaaattc tctttcaaac tctagagctt
     9901 cttttttagt tttaaaacct ctcttaattt tctttttcct atccccatcg aagtctgtaa
     9961 aataaaagct agcataccaa ctttttcttt gttcgtcttt gtaagcaggc attttagaca
    10021 cccccaatta tgtcatttta atattacaat aattataaca tgttagatat gctatctaaa
    10081 taattgtttt attttcctct tctttttatt tcttgaagct tgtaatattt acacagttct
    10141 aagaagtctt cttctagtgc tatagataag tctaatatcg tagatatacc tatatctttg
    10201 tactcttgat tttctagttt tgatatataa cttctgttac gtctcatttt ttctgctaat
    10261 tctagttgtg tcattctttt cttttttctt aattttttca acatttatta aaatccacct
    10321 tttaattatt tttatgacat ttttataatt aatagtttgt gtaatttctt gtaaaaaatg
    10381 ttcctcatta gaacattttt tggtggaaaa atgtgttaaa atgtaagtaa gaaaagcact
    10441 ttcaaagcac tataaaaata gtgctgaaat agataaacaa ataaaagtaa taaggaggaa
    10501 agatagattc tagagggaaa tcattatcat ttttaagaac gtatgttcgg ttggtgggat
    10561 agaaaaatac tttacagggg atggtttatt tgaatgagaa tgaaaatatg agtttatatg
    10621 tcagtaagtt aaaagaagta ttgaaaaata attcagaaga gtataaaaaa ataagagcta
    10681 aaataaatga aatttataat ctaaatgaaa agaaagaata aaagggcttt agctctttta
    10741 ttctttctta gtattaactg ctttataata agcattatct atcatttttt ttattgcatt
    10801 tttatcatct gaatctaaag atgctaatct atttattagt tcgttaactt cattatcttc
    10861 ttttgagtta acttttttta ataattcatc atcttgttga gtgttaggtt gtattttacc
    10921 taatttagtt aatagctcct ttaaagttaa atttaatcca aaactcattt tttcaagcat
    10981 atctaaagta ggttcaacag ctttaccatt ccttgggtct ctgttttttt ctatcttatc
    11041 tatatatgta tggctcacac cacaaagatt agaaaattct ctaagagata agttatgttc
    11101 acttctatat tctttaataa tttctgctaa tgtttgcata atttcacacc tcatctaagt
    11161 aatagttgac ttaattatat cagaaaaatg taaactattg ttaacaaaaa taaaaattta
    11221 gtaaaaaata attgacaaga gtaaaaaaaa gacgtatact atgattaaca aaaaggttat
    11281 aaataaagtt aggagggatt aatataaaaa ataatttaca atatattaga aaagaatcta
    11341 gaatatcaca aaaaaaattc gctgataaga taggtatatc aagaccgtat ttatctagaa
    11401 tagaaaacgg taaggttaat ccgagtttag aaatagcgca taaaatctcg ctcgaaacag
    11461 ggaaaacaat aaatgaaatt ttttttgatt ttactgtaaa tcatagtaaa ctataaggtt
    11521 ttaattgtac cttgaaaact aaatataaaa tattttaaag gagcaagtat atgaaaaata
    11581 aagaaaaaga aataatcatt agaggaattt taataggaat tttttatttt ttaggaattg
    11641 tatttagtaa tacttttttt aaataataaa tagaaggggt aggtgaaata ccaactcccc
    11701 aaatcaaaat aacactttgg aagttaaata taaaatattc aaaagaggtg attagatgca
    11761 aagagagcaa acaacgcttc gtataccaga agacttgcac aaagcgttga tagatttaag
    11821 cagtgatata ggaatgccta ttacatctat tataataatt gcatgttggt tgtatatatc
    11881 aaagataaat taaccaatcc gatatgcaaa atgaatgcac aacattattt attagagaat
    11941 tgattgataa attattttct ttagctgttt tctctagcaa ctcttttagg acaccaggaa
    12001 tcctaagagt agaacgaaca gtatcatcac ttttatacga gattgattga agttcattaa
    12061 cttttgaatt tctaataata tcattaatag cgtatagaat aagcgaagat ttgagaatgc
    12121 ctgtttgata agaaattttt tctaaatttt catttaatgg tgtatatatt cttacagtaa
    12181 tagtagtcat tttgacacct ccctaatgac attatacaaa aaatatttaa aaaagtcttg
    12241 acactaaaat agtgtcataa tataatgatt atagacagta aaacagtgtc ataaggaggg
    12301 aggaataaga atggaaaaag taagaatgac agtaaggctc gtcccaaaat taaatcaata
    12361 tgtacagaat atagccaaag aatgtggaaa aagtaaaaat tctattattg tggatgcatg
    12421 ttgggagttt attgagaaaa taaaaaaaga aaataaattc aatgtagaaa gcgaggaata
    12481 aatatgaata atctacagat atttgaaaaa ttggaatttg gacaaataag aatggcagag
    12541 attgataata agccttactt tgtaggaaaa gatgttgcaa agtcattagg ttataaaaat
    12601 actaatgatg caattttaag acattgcaaa ggggtagtga aacacgaggg gtttaaaatt
    12661 aatggtatta agattgcttt gataactgag ggtgatgttt atagattaat agtaggttca
    12721 aatctaccaa atgcagaaaa gtttgagagt tgggtgtttg atgaagtgct tccaagcata
    12781 agagaaacag gtcaatacca agcacaacag catgtaataa cagaacttac aggaactata
    12841 ggagacttaa aaggaacaat aaaagaatat aaaaagttgt gtaagataac atgctctaag
    12901 aaacaacagt attctaaata cataaagaat agactggata tagacaaagc taataaagaa
    12961 tacaaccaag tgaaagcaag gctattccta attcttggag ttgagaagtg ggaagacata
    13021 gacttcgata catctaataa tttaatacaa attatagatg attgtataag agttataaag
    13081 tcagagagac catacaagca gttgagttat tttgaaaact aaatacagaa tattttgaaa
    13141 tatattgttt taattaatta ttaactagga ggttaaatac atgaaaaata atataagcga
    13201 ttcaagagta aaatattttt gcaagtgtcc atactgtggg tttgataatg aggtagaagt
    13261 taaaaaagga ttgaagccta aaatatgttg catatgtaca aaagaagttg agtatgaaaa
    13321 actggagcaa caaagtactc cagcaaaata aaattaataa atatgaatat ttttacaaga
    13381 atctaattct ttttctaact tattaacagc atggttatat tgcagtgcct gttctgctat
    13441 aaagttagaa tttgatatgt gttctttaca catagggcat ggtagcccat tatcacaaac
    13501 ttccctaaat ttagattctg ttatggtaac ttttgtacca caatcgaaac aagtagcttc
    13561 aattttagtt attttaccga tattcataaa tcaccaccta atcatatatt tataggattt
    13621 atcctacaaa tatagtatat caaaggaggg aaataatggc aattaatgac aacataaata
    13681 aaattttaag agatagaaat ttaaaagcgt ggaaattagc aaaagaaata ggcgtagatt
    13741 caggaaattt atatgcaatt ttaagaggag aaaataaaaa tccaactata gatacattga
    13801 taaaactagc tgactattta gatgttacat tagacgaatt agttggaaga tagaaattaa
    13861 atacagaata ttttgaaagt aggtgtattg tatggcaaaa gcagtagcta aagaacaatt
    13921 gttttatagg gcaaaagata tagctaagat tttagatgta tgtgaagcaa ccgcttataa
    13981 aataattgga gaattaaata aagaattaga aaaagaaggt tggaagacct ttcctggaag
    14041 ggtatcagta acttatttca aagaaagata ttgttataaa acaaaaaaag gggcataaaa
    14101 aatgctatga gagtagctaa agatatgttt cctaaagtaa ttgtataaag aggaaggggg
    14161 tgatttagtt gaatgtaaga gtactgatag cttatataca gttttgtaag caatacaata
    14221 agaaagcgag ttttgaaggt cttaaaaaat acaacaaagg ggtaattgca tgagaataat
    14281 ttataaaaac aaaatctaca aagtagaaca agacaaagtg ttatttagaa ttacatacta
    14341 tgatgagcag aaaaataaca ggaagtttaa taacaataag aaagtaaaaa gaagtgtttt
    14401 aacaagagat atagagttag ttaacttgta tttaccagca catttaaaaa taaagtagga
    14461 ggtttaattg catatggtat ttaatttaga gaagttcaaa gtgggaaatg cgataagaat
    14521 aagttgtgaa agatttggtt ttgaaattga ttgtattgta gtagtagcaa ctgaggagga
    14581 actaaattta gcttactttg ataaagaaag aggttgcata gagtatcaag cattgatgcc
    14641 agaagacctt agatatgatg attatattct cgaaagatta ggttagaggg ggtgtatttt
    14701 aatggaaaca gctatatcta ttgcaaaagg tcaaatagaa ggagcgaatg agagtataaa
    14761 ggaattaaaa tcaaaagaaa attacgataa agaaagttta agatggtggg aaggtgttaa
    14821 gcaagctagt gaaaatatac tagagttttt agaaatagaa aataaggcat aagaaaagag
    14881 ccattacgat tggctcaatt caaaaacata ataaaattta attagctata ttatagcaca
    14941 agggggaact aaaatgaaag caattttatt aaaaagttta aatatagaga atttcaaagg
    15001 gataaaagaa cttcgcatag attttaacaa cataactaat gtgtttggag aaaatgcaac
    15061 aggtaaaaca agtatatttg atgcctttac gtgggtaatg tttgacaagg atagcaagaa
    15121 tagaagcgta tttgagataa agcctttaga caagcaaaat aaagtcatta gaggacttgt
    15181 aacaactgta acagcagtgc tggaagttaa taataaagag ataaagttaa caaagaaata
    15241 tgaagaaaaa tggactagaa aaagaggaga atcagaagca acttttacta aaaacgaaac
    15301 aacatacatg ataaatgata ctccaataaa aaagagtgaa tatgtaaaag aggtagctga
    15361 aatagcaaac gaggaacagt ttaaattact tactaatccg tatttctttt cgaatgagct
    15421 taactggaaa aaaggtagag aattaattct aaatgtgtgt ggagatataa cgacagaaca
    15481 aattatagaa tcaaataaag atttaacttt acttattaca gagtttgaaa aggaaaataa
    15541 catagataag attattaaaa atagaaaagc tattaaaaat aacttatcta aagaaaaaga
    15601 agaaatacca gttcgtgtaa atgagtgcaa taaaagcatg tatgacatag attttgagga
    15661 aatagaggct caattaaaag ctaaaaaggc tgacttagaa gctattgagg acatgttact
    15721 aagtggcaca aaggcgaatg aacagatttt aaaagataaa gaaaagatat ttgagctaaa
    15781 acaagagaca caaagtatta aacaatcagc aagtgaaaaa ggaaataaga aaaggaatga
    15841 gctgttaaaa gaaaaggatg agttgaaata taatattaaa aacttaagaa ataatcttgt
    15901 ttatttagaa agggataatg agttaaaaga aactttgaaa aaaagagcga ttgaaaaaac
    15961 aacaaattta agagataaat ggactaaaaa gagtcaggaa acattagatt taagtgttat
    16021 ccagacagaa tgcccaactt gtaaaagacc tcttgattta gaagatatag aagaaaagaa
    16081 aaaggaaatg ttagataatt ttaatctaaa taaagctaaa gaattaaaag aaatagcaga
    16141 gctagggaaa tctaaaaatg atgatgtaga attatttaat acagaaattg aaagattaaa
    16201 agtagatata aataagactt tagaagagat acaagaaaaa gcagtattat tagataaaat
    16261 aaaaaaagaa ttagaaagta caaaatctac agagatatat tcagcagatg aggaaaaaag
    16321 attaacagaa attagtgcag aaataaaaga gttagaagaa aaaataaaca ataaagatgc
    16381 tgataaaaat atagatgagc taaaagaaaa taagaaaaaa ctgactagtg aaattgagtt
    16441 acttaataaa gaactagcta aaagagatat taacaaagag ttattggata gaaaagagca
    16501 gttgttagaa aaagaaaaag cgttaggtat agaactagct catcaagaga aaatactcaa
    16561 cttatgtgaa ttatttataa aaactaaagt tagtttatta gaaagtaata taagtagtaa
    16621 atttaagaat gtaacattta aactatttaa agagcagata aatggtggta tcgaagaaac
    16681 ttgtgaagct ttaataaacg gtgtaccttt cagcaatgcc aacacagcgg ggcaaataaa
    16741 cggagggctg gatataataa atactttatc taatcacttt gaagttaaaa tgccaatatt
    16801 cgtcgacaac agggagagtg tgaataattt gattgatatt gatagccaag tgataaattt
    16861 gattgtaagt aatgataacc cattaaaaat agaaggggtg aactaaatgg cagaaagaaa
    16921 tataactgat gtagttttaa atagagtgaa tgagctaaaa gatagtggtg agttagtaat
    16981 tccacccact tattcagcag taaatgcttt aaaatctgca tacttaattt tacaagaaac
    17041 acttgataag agctataagc ctgtacttga aacatgtact cctattagta taatcaattc
    17101 tcttttgaac atggtcatac aaggattaag cccagctaaa aaacaatgtt actttgttgc
    17161 ttatgggaac aaattgcaac ttatgagaag ttatatgggt actatagcag ttactaaaag
    17221 actttctaat ataaaagata taaaagcgta ttgcatctat gagggtgata aattcaaagt
    17281 caaatacaat aaagaaaatg ctgtactaga aataggagaa tatgaaccag cttttgaaaa
    17341 catagatata ggcaaaataa aaggtgcttt tgctgtaatt gttggtaatg aaggaatctt
    17401 gcataccgaa ataatgacct ttacacaaat tgaaagagca tggttacaag gtgttggcta
    17461 tagtaatccc aaaaataaag tacatgataa ttttacagaa gaaatggcaa agaaaacagt
    17521 aataaataga gcgtgcaaga tgtatgcaaa taccagtgat gatagtgatt tattaatagg
    17581 tgcttttaat gaaactgata gagtaatcac tagcgaagaa attgctaaag atacagacaa
    17641 agaagtagaa aaagagattt ctcaaaatgc taatatcaag tctttagaaa tgccttcaca
    17701 agtggataat acaatcttta ctcaaactaa agaaaaagta gctattaaca tagatgaaga
    17761 taagccgaat ctagctgaat ttgaagaagc aatccaagtt gaattggata caccaccatt
    17821 ttaaatatga ttaagatatt agggagcagt agcagaggta attgctactt gctccaactg
    17881 gaaaacgaga cattaatttt agagtgtgga gtcagttaca aagagatatt aaaaggctta
    17941 gatttcagtt tagaaaatgt tgtaggatgt ttggtcacac atgaacataa agaccactct
    18001 aaagctatca cagagcttac aaataatgga atagatgtat attctagcaa gggtacatta
    18061 gaagctttaa aaatagaaaa tcatagaact aagattatag aaagcgagaa attattcaag
    18121 ataggtaact ttaagatagt gccatttaat actaagcatg atgcggtaga gccattagga
    18181 tttttaataa atcatagtag ttttggtaac ctattattta taactgatag ttattattgt
    18241 gaatacaact ttaataattt aaatcacatc atgattgagt gtaactatag taaagattta
    18301 ttagataaca acaaagataa gatttatttg agaaatagaa tagtcaaaag tcattttgaa
    18361 ttaagtaatg tgattaattt tctaaaagta aatgatttaa gtaatataaa aacaattaca
    18421 ttgcttcatc taagtgaaga taacagtgat aaagatttat ttataagaga gatagaaaag
    18481 aatgtgggag taccagtaat agtagctgaa aaaggattag aaatttattt agactagagg
    18541 tgataaagtg gcaaaatata gaattttaca agctaacttt tgggatgatg gatttgtatt
    18601 agatttaact cctgaagaaa agtactttta taactatctt ttaactaatg gtagagcaag
    18661 tcaatgtggg tgttatgaat taccctataa aattatggaa atgcaaacag gatataacag
    18721 ggaaactgta gagaagttaa ttaaaaggtt tatagagtat ggaaaaataa aatatgactc
    18781 tactactaaa gagatattaa taattaattg gagtaaacat aatttttcta aaagtccaaa
    18841 agtacgtact tgtatcttaa aagaggttga atcaattaaa aataaagaat ttcagcagta
    18901 tatgtataga gtatgtatag actatggata ccctatcgat acagtatcta tagactatgg
    18961 ggaaaaagaa aaagaaaaac aaaaagaaaa agaaaaacaa aaagaaaaag aaaaacaaaa
    19021 agaaaaagta gaaccgttaa tttgtgaaga acaggaaaaa aatgagttaa aagaatttaa
    19081 gaagctatat gaagaaaata taggagtggt atatccagtt acagcagaat ggttaataga
    19141 aatttctagt gatgtagata taagattatt taaatctgct atagagatat gtgctgaaaa
    19201 gatgaatatg aatatagcat acttaaaagg tatccttaaa aagtggaagg atgcgaatat
    19261 aactacatat gaacaactag aatcatatag attacagcaa gaaaataaga aagcaaaaaa
    19321 agtagttaat ggccaactaa gtaaaaataa gtttgccaac tttgaacaaa catttactaa
    19381 gtatacagag aatgagcttg ataacataat taagaaaagt caagatgcta agttcaaata
    19441 aaattaaact tctaggaagt aaatatcaat atattgcttc ctagaaaggg ggtagaatat
    19501 gggtaaaatt tggatggatg caggaacatt tttggaaact aaagatatag gagatattga
    19561 atgtaatcta agagaaatga taaataaaaa taaacaaaaa aatttaattg gtagaataaa
    19621 ttccaaagga caaggaagaa aaagtaagaa agtagagtgt actaacatca ttactggaga
    19681 gagtaaaata tttgtcagtg ctgtagaagc aagtgaatat ttatatttca cggaacttca
    19741 tattactcgt ctagcaagaa caggaaagac tactaaaaat ggttggaaag ttagatatat
    19801 tgaagaggtg tcaaatggta ttagcaaatg tggaacaagt aattaagtta gctgaaaaga
    19861 tattaagtaa gaaaaagtgt tctgttaata aagctattga tatagctata aaaatattaa
    19921 atagatacga atatgagggg atgattaaat gagtctaatt gaatatagag gttatgattt
    19981 tgaaaataaa aagtggattt attctaaaac aataatgtgg agtaatgcag tagactgttt
    20041 attaatgctg aaagaagatt ttaaatggcg aaaagtttgt aatgttggaa tatgttctga
    20101 agaatgggct ggaaataatc aagaaatttt tgaaggggat atattaaaag aaaattataa
    20161 tcctcacaat agaagttcat atggaattgt aaaaagagat tttaatagta ttaagttata
    20221 tttggagtgg cattatttaa agaagtttga aggagaatgg atagagatta tagataaaac
    20281 agaaatatat catagtagag gttacaaagt aattggtaat gaatatgaga acttagagga
    20341 agttaggaaa gagtttttag aacgcaagga gatgttggaa aatgaacgtc ttagctagtt
    20401 taatacttat agtaggaagt tttatagctg gtagggttta tgagtataga ttgaatctaa
    20461 aagagtttga aaattatgat aatataggag gttttaagaa tgaatgatag attagaaatg
    20521 ataaatgctt ctgtaaatta tatacagatg atatgtgaaa gttcaaatat agctattata
    20581 gcagagcatg accgagttag aatattggat ttagaaacta aagaaaagta tgacctatta
    20641 agaagtgagg gggattaaaa aatgagtaaa gttatacaat gtgatttctg taaggaaata
    20701 tttgaaaaat ataatggaga atgtattgag ctatataaaa aggattgtag caaaggggtg
    20761 cttgggatag ataaacacat atgcccaact tgttatgaaa aattcattga aggaaaaata
    20821 gagaaagtag aaaatgctaa aagatttaag gataaattaa tagacttctt ggttgagcat
    20881 gaggtttgtg actgttgtgc ttgcgactgt gtgtttgatt ctaatgacga aaaaaaatgc
    20941 aaggcaggaa ttagagagtg ggttgaaagt gaggtagaag aataatatgg caaaattaat
    21001 agttagcata cctttaacta aatatttaaa agatataact aaagaatggg gagaaactta
    21061 tataactaaa acatatggtg ggcatgtcta tttaagtgat gaagctccag gagaagtatt
    21121 taaaaaagaa gatgatttaa attatataga gtttaacgat tctgatattg tcatgagatt
    21181 acctaaagaa gtacatcaac atataaattt gaaaaaaggt tgcaacaaga atcttaaaga
    21241 tgtattaaaa gaaataaaaa agatagaaga gaaaacaaat atatagaaaa tatctaatta
    21301 aaacagttta gagagttgca aaatatcttt tagtataatt tattgttgaa gtgttttgtg
    21361 actctcaaaa atgaaat
//
LOCUS       DAMJZE010000051        21158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJZE010000051 DAMJZE010000000
VERSION     DAMJZE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.051"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            551..1582
                     /locus_tag="PC541_001701"
     CDS             551..1582
                     /locus_tag="PC541_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2839168.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1715..3013
                     /locus_tag="PC541_001702"
     CDS             1715..3013
                     /locus_tag="PC541_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2839169.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3127..3372)
                     /locus_tag="PC541_001703"
     CDS             complement(3127..3372)
                     /locus_tag="PC541_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2839170.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3520..4386
                     /locus_tag="PC541_001704"
     CDS             3520..4386
                     /locus_tag="PC541_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2839171.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4390..4926)
                     /locus_tag="PC541_001705"
     CDS             complement(4390..4926)
                     /locus_tag="PC541_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2839172.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5117..5320)
                     /locus_tag="PC541_001706"
     CDS             complement(5117..5320)
                     /locus_tag="PC541_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2839173.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5611..6375
                     /locus_tag="PC541_001707"
     CDS             5611..6375
                     /locus_tag="PC541_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2839174.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6764..8077
                     /gene="prdC"
                     /locus_tag="PC541_001708"
     CDS             6764..8077
                     /gene="prdC"
                     /locus_tag="PC541_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2839175.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8482..8567
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9149..11323
                     /locus_tag="PC541_001709"
     CDS             9149..11323
                     /locus_tag="PC541_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2839176.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            11730..13490
                     /gene="prdR"
                     /locus_tag="PC541_001710"
     CDS             11730..13490
                     /gene="prdR"
                     /locus_tag="PC541_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2839177.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13919..15799
                     /gene="prdA"
                     /locus_tag="PC541_001711"
     CDS             13919..15799
                     /gene="prdA"
                     /locus_tag="PC541_001711"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2839178.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15880..16164
                     /locus_tag="PC541_001712"
     CDS             15880..16164
                     /locus_tag="PC541_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839179.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16202..16927
                     /gene="prdB"
                     /locus_tag="PC541_001713"
     CDS             16202..16927
                     /gene="prdB"
                     /locus_tag="PC541_001713"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16652..16654,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2839180.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            17007..17765
                     /gene="prdD"
                     /locus_tag="PC541_001714"
     CDS             17007..17765
                     /gene="prdD"
                     /locus_tag="PC541_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2839181.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17780..18247
                     /locus_tag="PC541_001715"
     CDS             17780..18247
                     /locus_tag="PC541_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2839182.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18265..18747
                     /locus_tag="PC541_001716"
     CDS             18265..18747
                     /locus_tag="PC541_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2839183.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            18767..19774
                     /locus_tag="PC541_001717"
     CDS             18767..19774
                     /locus_tag="PC541_001717"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2839184.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19894..20775
                     /locus_tag="PC541_001718"
     CDS             19894..20775
                     /locus_tag="PC541_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2839185.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8443 a         2444 c         3877 g         6394 t
ORIGIN      
        1 gtattagttg ttaaaataat atttatatta gtttttatac atacttggta taagtttttc
       61 atatattgat taaaattgat gttgatttat tatttttagt attttttcac cttttgattc
      121 atactttgta gaatggtgta ttttctaaaa ctgattataa gacaagttca ttcaaattag
      181 catttttata tcatataaat aattgttccc cctaaataag atttagatgt atagtattaa
      241 aatcaaaaag tataaaataa aattgatgct taatcataga taaatattaa taaaatatac
      301 aaggggattg tctaataaaa agataatctc cttttttaca aataattcta aattgtgata
      361 taaatctaaa aattttatct taacatttaa ccatttaatg ataataattg atataataaa
      421 tataaattaa atatatggtg tacttatttt atgtttttag caagaaatat agcatttaaa
      481 agataaattg tgcaacttaa atcaaagttt aagatgaact attaacatag atatattaaa
      541 aacttttgta atggagaata tatgtgatat gaataataaa gttaaggttg cagtattaga
      601 tacaggaata gacaaagaac atgattactt aaaagataat ttagttggag gaattgcttt
      661 tgaatgtatg catgattata tctttatatc agataaattt gatgatgaag atggtcatgg
      721 aactgcttgt gcatcaataa taaagaaaga gtatgaagat gtagagttat ttgtaataaa
      781 aattttgaga aaagatggta ttacaaatat aaaagttctt gaagaagcat taagatacct
      841 tttagacact aacataagat taattaattt aagtttatca gtcataggag tagaaagtgt
      901 taagggctta tttgaagttt gctatgaatt gtttagaaaa ggaaaaataa tagtatgttc
      961 actcgctaat gattttgact taagttatcc agctatgttt aacaatgtta taggtgtaag
     1021 aggatttact ttagatggag atgattcatt ttggtacaac aaaaaatatg atgttcaatg
     1081 tgttatggat agcaattcat atataagttg tgatataaat aattcctata gattaccacc
     1141 aaaatgtaat agttatttat cagcaaagct tactggtaaa atagctaaga tattatcaga
     1201 aaaaccaggc ataacaatat ctgatttaaa tgataagctt gaatcattag caactagaaa
     1261 tcattgggat agttgtgatt ttgatgagtg tagtagaatt gtaaacttta aattggattt
     1321 gtatgacaaa gaaaatgctc tttttatgaa agttgctgac gttgttaggg attgcttaga
     1381 tattgaagag aataatgaaa aactctttaa gggtagcctt tttaataagg agataggctt
     1441 ggtttatgat aattgtttta atctgttgaa aaagctagag catagatttg acataaagtt
     1501 taattatatg gacatctcaa aatatgactt agtttcaata tatacactga ctgaattagt
     1561 ggaaagatat aaaaacttat aaataaagat ttgtagctaa atttaatgta gaaggtatta
     1621 aattatatgt attctttgtc ggtagattaa aagtaaaaaa tatactataa ttagatagca
     1681 taagagaaaa tgccaataca gggggagtta gataatgaaa tatgaagaag cattagagta
     1741 catatcacaa actaataaat tcggtataag attaggatta gaaaatatcg gaaagttatt
     1801 agaattatta ggaaatccac aagaaacttt gaatataata catgtagctg gaactaatgg
     1861 aaaaggttct gtgtgttctt ttgtttctaa tatactaaga gagtgtggat ataaagttgg
     1921 tttatataca tctccatatt tagaaacttt tactgagcgt ataagagtaa atggacaaaa
     1981 cataccacag gatgatgttg ctagaataat tgagttaata aaagaaaaaa tagagattat
     2041 ggtaaaagaa ggctatgcat atccgacaga atttgaagtg gttactgcaa tggcatttta
     2101 ttactattca gaacaaaagg tagactttgt ggcattagaa gttggtttag gtggcagata
     2161 tgatgctaca aatataataa caaaatcact tgtaagtgtg atagtatcta taagtttgga
     2221 ccatacaggt atacttggag atacaataga aaaaatagcg tacgaaaagg ctggaataat
     2281 aaaggaaaat ggagtagtac tagtatatga ccagactgat gaagcaaagg atgttataaa
     2341 gtcagtttgt aaagagaaaa aagctaaata tatagaagtt gattttgatg atataaacat
     2401 aaaaaaatca gatataaatt ctcaaatata cgattgtact gtaatgaaag aaacttatag
     2461 agaccttgaa ataaaattga taggcgaaca tcaaataaac aattctatct tggcaattag
     2521 tgtaataaaa tatctaaaag acataaataa attagcaaat ataaatgaag aatctataag
     2581 aaaaggactt ataaatacta aatggccagg aagaatagaa aagataaagg aaaatccaat
     2641 ttttataata gatggtgcac ataatgaaga tggagcaaaa tctttggcaa aagcacttga
     2701 taagaatttt aaaggtagaa aattaacctt attaataggt atgttagaag ataaagatat
     2761 tgatagtgtt ttagagatat tgttgtcaca ttttaataag gtaataacaa caacaccaaa
     2821 taacccaagg gccataaatt cagatatatt aagagaaaaa gtattaaaat atgtagatga
     2881 tgtaacatca aagcatgaga tagaggatgc agtaaattat acattagaaa catcaagtga
     2941 ggatgatatt ataataagtg caggttcatt atatatgatt ggtacagtga gaactttagt
     3001 aaagaaatta taattttata aaataagtat acaaataaaa atataaaaaa gagttgtcta
     3061 aaaaatggac aactcttttt atataaaata agtttttaat aattttactg atgtttatat
     3121 aagtaattat atagattgac gtatagcttt agataattgg tctgggcaag aagtaccttt
     3181 tccaccacag tcaataccat ttaatttatc agcaacttca agagcatttt ggccaattat
     3241 taagcttcta agtcctgata aattaccatt acatcctcct ataaactcag catcaacaat
     3301 cacattattt tcatcaactt caaaaatcat ttctctacaa catacaccac ttggattaaa
     3361 tgttactttc attaaaaaga cctccattaa atattatttc tttttttaac attaataata
     3421 ataccaaata tatgcgtaat aatgcaacta taaagcataa tacaagctaa tgaaataata
     3481 tattattgta gtatatgtat tgtagagagg aggggttata tgaaaatcta tatttctaac
     3541 tacttatcaa agagtattag catagttgat tattctacac ttgaactaga aaaagaaata
     3601 gtattagaag ataatatata tccacaccat ttttgcatag aaaaagaaaa aaatttaata
     3661 tacataccta gttcaagtaa tgggatactc tatgtcttag atttaagtaa tgataaaata
     3721 attgatactg tctctatagg aggaagtctt agtcaagttg tacttagcga taatgaattg
     3781 tttgtggcaa atgaagattc taatagtata tatgtactag ataaaaacac attaaatcca
     3841 ataggaatta ttggagttga taatatgcca catggatttg actttgatag agaaagcaaa
     3901 aagttatatg taccgtgtat aaactcgata gtatgtatag atacaattaa taaaaatata
     3961 caaaagaaaa taaatatgga ctttagagct tggcatatag aacttgataa acaaaagaaa
     4021 gaaatgtata tatcaacact tgatggaaag cttataataa tagatgaagt aagcatggat
     4081 ataaaaaagg tattatatga atttttatta ccagtagaaa taagatttaa ttatagtggc
     4141 aaaaaagttt atgttgctga tttgggttat aataatgtaa gaattttaga ttatacaacg
     4201 agtagataca ttggaaatat tgaaataaat gggattcccc aaggattaga aatatctaaa
     4261 gatgaaaagt tactatttgt atcagataca caagaaaatt cagtaaaagt ttatgaaact
     4321 gcaaataaca aattaataaa agaaatcaag gtaggaaaag agcctacaac tataatttgt
     4381 ttgtaggctt taatgtatgt ctcttataag taaatcgata acttcggcat acatataaga
     4441 tgcattattt ttccagttat tgcaaataag ttttgcttgt ttctctgatg aaacatttag
     4501 atttaaatct attaagttag actgattttc tatgactcta aggttgacaa taaattcatt
     4561 atcactttgt tttacaaaac tagactttac ttgagtatct gctagtagat tttctttgtt
     4621 agaatctata tacttgtcta ttttttcagt aacactagta ggtattcgat taaagaagta
     4681 ttctagactt tctacgcctc tttgagtaag agcataatat tctctgtcag agtctttata
     4741 tgtagtcaaa aacttagatt ccataagctg agataaaagc tgttgtaatg aaaaataatt
     4801 catcatctca gtctctaata ctacttgagt tatttgagaa ttagttaaat ccattttaat
     4861 tttttctaat atatataaaa ttaaaagttt gtgataggct aattcttcag acgagttttc
     4921 aaacataaat atcatcctcc gtcaaactat ttctatatat aatatattaa actaatttgt
     4981 acaaaattaa aagaacaatt tgtattgtat tattaatctt taataattca tataaaaaca
     5041 attttataaa taaatataga aaaacagggc tgctaattag cagccctgtt gatacccagg
     5101 tgtcaaaatt tactatttat tttccagcca tttgtctttc agccatttca actaatttct
     5161 tagtcatata tccaccaaca tatccatttt ctctagctgt taagttacct ttgtctatgt
     5221 tttcatagtt agataaacct atttcgttag ctatttctaa tttcatttgg tttaaagcag
     5281 cttttgcttc tggaactact gttctgtttg aatttgacat agtaaatttc ctcctcaaat
     5341 aattagttta tgatgtctaa caacactttt atttatgtga tgttaatact aatttctcca
     5401 aaataaaaat aatatataca gaaaaataaa ggtaaaaatg taaaaaatat aaaaaaatta
     5461 aataaatgag tattttagag agtttaatta ggtcaatata ggttttattt ttttagttat
     5521 tgtcttataa attaaatatt acagtacttt tttgagctga aaaaaaataa attttaaata
     5581 tagaagaaat tatttcaaag ggggaatagt atgaaaagag aagttaaaag gactaaagct
     5641 ttgatatata gtgttttatt tttttcatta ttattagtgt taatatttgt ttttgtttat
     5701 ggaataaaat acatatgttc taaatctgac aattcaaaaa tgcctatata taaagttgat
     5761 acaaatgaga aaaaaatagc tttgagtttt gatgtagcat ggggaactga taatatggat
     5821 gatattttaa aaattttaga taaacataat gtaaaagcta ccttcttttt agttggtagt
     5881 tgggtggatg ataatgaaga aattgtaaaa gctatagatg aaaaaggaca tgaaattgga
     5941 aatcattcaa atactcatgc caatttgaag gaaatatcaa aagaagacat aacaaaagag
     6001 atagaaacta catcagaaaa aatatataat ataacaggaa aaaggactaa tttatttaga
     6061 cctccttttg gggacgtaaa caataaagct atggacattt gtagtgattt aggttataaa
     6121 gtaattaagt gggatgtaga ttctatagac tggaaggagc ttggaccaaa tcatgtaatt
     6181 gaaaaagtaa taaaagaatc tcaacctggt tcaatagttt tattccatgc taatataaat
     6241 gatgtagata actatttgga tactataata agtcgactaa aaaaagatgg atacagtcta
     6301 gtcaaaatat ctgatttgtt gtataaagat aattatgtag ttgattccaa tggagtgcaa
     6361 aaaactaaga attaataagt aataaatgta tattttaagt aaagttgtga taaattatat
     6421 agtatatgtt agaaaagtgt cgataaaaag acggtgtcta ttttttgaca ctttttatac
     6481 atgtatgcaa tatcaataat tatagattaa attgattaaa aaagtgtcta ataattgact
     6541 tttttttgat gggtttaaaa atttagaaga attaatgaat gctgtaagac agtatttcca
     6601 aagggtataa gtttgtttaa aagttggcat agaaattgct ttgttaataa gcgatatgtt
     6661 ctatagaaat aaacaatatg ttaagacctt ataatagaaa aatagcatta acattatttg
     6721 aacagcttat aaaaaaatta atatacttaa gggggcgtac tagttgaaca aactatattc
     6781 tattttatta aaacaacacg ttggtggtcc agataagcca gtagtaagtg ttggagatgt
     6841 agtaaaaaaa ggaactctaa tcgcagaacc aactggatta ggggcaaata tttatgccag
     6901 tgttagtgga aaaataagtg aaataaatga ccaagcgata gtaattgaag ctgatgaagt
     6961 acaagaagat acttttgaac cattaaaagg agaaggtata cttgacttaa ttaaagaagc
     7021 tggagtagta ggaatgggtg gagcaggatt ccctactcat gtaaaattaa acatagattt
     7081 aaatggtgga acaatattag caaatgcagc tgaatgtgaa cctttattag ctcataatat
     7141 aaaagaaata gaagaaagac cagaaatagt ttatcaaggt ataaaatatg ctatggaagt
     7201 tactaatgct ggaaaaggta tgctagcaat aaaaagtaag catccaaaag caatagaagc
     7261 tttcaaaaaa gtaataaagc caggagacaa catagaagtt gcagaactag ttgatatgta
     7321 cccaatggga gaagagagag caatagtaag agacgttcta ggaaaattac ttgagccaac
     7381 tcaattacct tcagaggcta atgcagtagt aataaacgta gaaacactaa ctagaatagt
     7441 agaggctgtt gagcaaaaaa gaccagttat atctaaaaat ataactgtag ttggtcaatt
     7501 aaacagtgga aaagaatcta tagtgtttga agatgtgcca attggaacaa ctgtcggaga
     7561 attaattgaa agagctggtg gaataaaagg tgaatatggt gaaataatcc taggaggacc
     7621 tttcactggt aaagctacta ctttagatgc tccaataact aaaactagtg gtggtataat
     7681 agttactatg ccatttgtta atgaaaagag aaaaatgggt cttttagttt gtgcatgtgg
     7741 acctaatgaa gaaagaatga gagatatagc aactaaaatg ggagttactg atatagtatc
     7801 agttcaaaaa tgtaaacaag ctcaagaaat taaaggtgca ttaaaatgtg aaaatccagg
     7861 acactgtcca ggacaagctc aaaaatgtat tgaatttaag aaagctggag cagaagttat
     7921 attaatagga aactgtactg actgtagtaa cacagtaatg ggttctgcac caaaattaaa
     7981 attaggtgta tatcatatta ctgaccatgt aatgagaaca gttaatcacc cattaataag
     8041 aagaataaaa ggtgacgtaa gattaaaaat aaaataatag ataaatagaa gttagacatc
     8101 tttagaagta aaacttaaaa gttacttcta aggatgtttt ttaatgtatt ttgagggcaa
     8161 tatatatatt gtaaaataaa tggaaaaaat agacaaaata tgctagatat tgtataatga
     8221 taataatagc tattacaatt aatattggac aaaaataatg atactcaagt atgtggtttg
     8281 tattaatatg catattatat taagtcaact atccgataga gtaaaaggtt tttgtgatat
     8341 ttgaagatac tctatagtat cttttttatt ataaaaacta atttttgtct tttattagat
     8401 aagtatctag gatactaatt tatattttat tgagttgtta aaaaagtgca cttaaaaagt
     8461 taaaaaagaa aaattaaaaa acctatttat agaaactgtg aagtatatct taaacctggg
     8521 cacttaaaag atatatggag ttagtagtgc aacctgctat aaatataaat aggagtaact
     8581 tttaattgtc aaaaatatat aaaataaata cataaataag taattaatag aagatagata
     8641 taaatatatt tttgaaatgc tccctgcatt cataacgagt gtagagtggc agatatctgc
     8701 ccaaaactcc tttaattgct ggaagttcct aaagctaact aaaccacaac ataatcatga
     8761 aataaggata agtgtgatag agtgtgcatt taataagcac ttgaggttac gaaagtagaa
     8821 aaaattagtt agatagcata aggttaaatc ctaagtgcct tgaaaatgga taatcagcag
     8881 gtaagcttcg aaaagaagaa acttcaacga ctagagagaa atctcgtacc tacaagtgtg
     8941 gggaaatggg gagtacctaa tttatggtta ggtaatgata tagtctgtgc ttaatagaaa
     9001 tattaagaag ttcataggag aactggttag gagtaacgac cctaattgaa cacactaaag
     9061 atgtattatt gaataagatt catataatat ttaataattt taattatatt aagtgaatac
     9121 aatatatgtt tttagtatca ggagaatttt gaaaacaaaa attaaaaaat caagtataat
     9181 cagtttagta attgctttta gtatggtgtt tactgcattt acaccaatag tatcttatgc
     9241 tgacgaagtg actagcaatg atacaatact taatggagaa gaacaatcaa atggtcagac
     9301 accagatgta gagaaaccat cagacggtca ggtaccagat ggggagaaac catcagacag
     9361 tcaagtacca gatggggaga aaccatcaga cggtcaggta ccagatgggg agaaaccatc
     9421 agatggtcag ataccagatg gggagaaacc atcagatggt cagataccag atggagagaa
     9481 accatcagat ggtcagatac cagatggaga gaaaccatca gatggtcaga taccagatgg
     9541 ggagaaacca tcagatggtc agatgccaga tggagagaag acatcagata gtcagacgcc
     9601 agatgtagag aaaccatcag atggtcagat gccagatggg gagaaaccat tagatgagca
     9661 aactccagaa gaagaaaaac cattggaaga agaaatagtt atagaggaaa tgagtttaaa
     9721 acaagatatt gataaaattc ttgatatgac tttatctcaa ataaataaaa tagtatataa
     9781 cttttgggaa gatgaagaag atgtaaaggc agatgaacaa tcagaaatta accaagtttt
     9841 tacttcagaa gactcattta ttagcttatg gtatgacaaa aaagcaaaag taaaaaattc
     9901 ttgtttacta aaagaaatag atggttcaga tagaccttat catgatttaa gatttgatga
     9961 tattacaaag actttaactt ttgactatgt aataaagggt cttgtaggag caagctcaaa
    10021 agacgacaaa tatgtaatag aaggtgaaga tggagagcaa actgcatttt gttacaataa
    10081 tcatttaaga ccaccaagta gtaatggaaa gagtccatat ttaccagcag aagattttaa
    10141 tggtcaagaa aaccaaaatg aagaagcagt taaatctata ctatatgcag gttctgaatt
    10201 tgatggattt ggatataagc aacaatttaa tttaggtgga gaagaaaatg aattgatgac
    10261 ttactcagct actcaaagtg caatttggat tattcttgga caaatggatg aacaagaaag
    10321 acttaagcag tatcaaggtt ctataaacct ttgtgataaa cttatagaaa gagcaaaaac
    10381 tgaagaggaa agagctgagt atcagaagaa gaaagaagta gctataaata taaaagctta
    10441 tttagaagca ttgttaaaag caggtcgtga agagttaaag ccaaatgata caggaaaacc
    10501 tagtttgagt aatggtttaa ctaagattaa ctttgaaaaa aatgaagatg gtacttatga
    10561 aacagaagct gtagccttag taggttatag tggtgtagta aagttaagac taccagatgg
    10621 agtaactgct tatgatggag atggtaatat aataggtaca ggtgaagtag aaatttcaac
    10681 acaacagaaa tttaaactta agagtgttgt aaaaccagat gccaaagcta atatttctgc
    10741 tgtatcttat gattatattt ttccaaaagc tattcaatat tataaggcag tgcttgattt
    10801 aggacaaaaa gaccattcat cttcattacc atctagtaag caaaatttat taagttatac
    10861 aatagagaaa aaaaatggtc aagaagttaa ctttaatata ggtctaccaa cagatgatgt
    10921 tgtaaatcca ccagtgccac caacagatga tacaaatgga cataagccaa aaccatcacc
    10981 accaatagat gatacagtaa taaatccacc agtaccacca atggatgata caataataaa
    11041 tccaccagta ccaccaacgg aggatgcagt gttaaatcca ccagtgccac caacggagga
    11101 tgcagtgcta aatccaccag tgccaccaac agaggataca gtgttaaatc caccagtacc
    11161 atcagtaagt gatacagtgg aaaaaactcc agaactatca agagatgata caataataaa
    11221 atcaccaaaa acaggtgatg aaactcagct aatgtcatat gtatttatta gtgtcattgc
    11281 aatttgtgga ttagcgtatc aatgtaaaat aaaaagaaat taatctagat tattattaag
    11341 tttaaaataa aaagtctcaa attgatttat aagtcatttt ggggcttttt tatgtaaata
    11401 taatatgcaa atatagtttg tttaaaaata tatattttaa ttatattctt aaatttatga
    11461 ttggatacag gcattcatta attgtttgat gaatagaaaa atgttttgaa ggtgaaaata
    11521 gtattgaaaa aataatatac taagggtata attcatttga tttcatttgt ataaatttaa
    11581 ttataaattt taaagtgatt atataaattt aaatgctaat tacttaataa attttaataa
    11641 taattattta ataaataatt attataatat gctagaataa atatatataa ataagttttg
    11701 atgaggtact actataaagg ggggacttta tgttttcgat accagaagta aaaaaagtag
    11761 aagaggtaat ggatacaaaa tttacaacta tagacgaaga cacaagaatt gaagatgcaa
    11821 taaaagaaat gataaaaagc aacacaaaaa ctctaatggt aatagattca tctgaccaat
    11881 taaaaggcat tatatccatg acggatatac ataatcttta tgagatgcat aaaaagtatg
    11941 aaggtcaacc agtaaaactt ataatgaaga aggatgtaat atatgtcaac gaaggtttaa
    12001 ccttagatga atgtagagat ataatgatat taaaaaatat agggattctt cctgttctta
    12061 gagataataa aattattgga gttctaaaac aagagcacat tagggactat ctatatatgc
    12121 atttagaaga ttatggatta actttgaagt atattatagg tcaaattaaa gaaggaattt
    12181 gtgctataaa taacgaaggt gttgttatct tgtggaataa gtttatggag gaaaggtatg
    12241 atataaagtc tgaagatata gttggtcgac cgatgaatga atttttggaa aatactattt
    12301 cagaaaaagt actaaatagt aaagttggaa tgagtgactt gtattttaca gataaaaaag
    12361 aaaatatgta tgcattagtt catgcaaatc ctatatttta taaagaagaa tttatcggtg
    12421 ttgtatgtac agaagttgat gttacagaag ctaagatatt agctcttgaa cttgaaaaag
    12481 taaatgatac attaaaatat ctaaagaacg aagtcaaaaa tttatctaaa ggtagttttg
    12541 ataagatttt aggtaaaagt tataaattgg aaaaatcgaa agcgattgca aaacaagtgg
    12601 ctagaacaaa tagtagcata tttatttggg gcgaaagtgg tacaggtaaa gaagtatttg
    12661 caagagctat tcatgattat agtgagagaa aaggtcaatt tatacctgtt aactgtagtg
    12721 ccataccaaa tgagttattt gaaagtgagt tttttgggta tgaatcaggt gcgtttacag
    12781 gagcaagtaa aaagggtcga ataggtatat ttgaacttgc aaaagatgga actgtatttt
    12841 tagatgaaat tgccgattta cctcttagta tgcaagcaaa gttgcttaga gtattacagg
    12901 aaaaagaaat aagaagagtt ggtggagata ccacaataaa aataaatcct agaataatat
    12961 cagctaccaa taaagatttg agtaaaatgg taaaggcaga aaaatttaga gaagatttat
    13021 actatagatt aaatgttgtt gaaataaaga ttcctccact tagagagaga aaagaagaca
    13081 taggattatt ggttcatagc tttttagagg agatatgtaa acaaaataat aagcctgttc
    13141 ttacaattag caaagatgta atagatatat ttcagaatta tagatggaaa ggtaatatta
    13201 gagaattaaa aaatacgatt gaaaatattg ttgtattgtc acaaaattct aaaatagatg
    13261 tggatgatgt accaagttat atgatggatt ctacaaataa tagtactgaa gaagaagagt
    13321 atcctttaga tttaacgaag gcaactcaaa aaattgaaat aaagaatata accaaggcac
    13381 ttaaaatgtc taatggaaat aaagctaaag cagctaagat attaaatata cccagaacaa
    13441 ctttgtatta taagatagac caatacaaaa tagatgtgtc taaaatatga cacatgacta
    13501 aattttgaca aaatgataaa ctttaattta aaacctagta aagccaacgc ttatataagt
    13561 gttgtgtata tagatgtgtt aaaaaaaatg aaaaagtgtt gattaattga cacttttttt
    13621 gtttgagaaa atgtttacaa atacaaaaaa taacatattt taaataaata tttatataat
    13681 atttggctaa aaatagcgat agatgaagaa tctattatag aaaaaatctt tttgttaata
    13741 agttggcata ggaattgctt ataatataaa gtgactttaa gtgagaaaaa aataatatta
    13801 atcataacaa attatgtagg ttttaattta cttaatttct cagttaaggg gttacctgtt
    13861 gaggaattga gatttaaaat aataaataag aaaataatgt aaggaggtat tttatactat
    13921 gtcaataact ttagaaacag ctcaagccca tgcaaatgac ccagcagttt gttgttgtag
    13981 atttgaagcg ggaacaatta tagcgccaga aaacttagaa gatccagcaa tatttgcaga
    14041 cttagaggat tctggattat taacaatacc agaaaatgga ttaactatag gtcaagtact
    14101 aggagctaag ttaaaagaaa ctttagatgc actttctcca atgactacag ataacgtaga
    14161 aggatacaaa gcaggagagg ctaaagaaga agtagtagaa gaaacagtag aagaagcagc
    14221 tccagtatca gaagcagcag tagttccagt aagcacagga gttgcaggtg aaacagttaa
    14281 aatacacata ggtgaaggta agaacataag cttagagata cctttatcag tagctggtca
    14341 agcaggagtt gctgctccag tagcaaacgt tgctgctcca gtggcaagtg cagcagcaga
    14401 agtagctcca aaagttgaag aaaagaaact tttaagaagc ttaactaaaa aacactttaa
    14461 aatagataaa gttgaatttg ctgatgaaac taaaatagaa ggaactactt tatacatcag
    14521 aaacgcagaa gaaatatgta aagaagctaa tgaaactcaa gagttagttg tagatatgaa
    14581 gttagaaata ataactcctg ataaatatga aacttacagt gaagctgtat tagatataca
    14641 accaatcgct actaaagaag aaggcgaatt aggttcaggt ataactagag ttatagatgg
    14701 agctgtaatg gtattaactg gtacagatga agatggagtt caaataggtg aatttggttc
    14761 ttcagaaggt gagttaaata ctactataat gtggggtaga ccaggtgctg ctgacaaagg
    14821 tgaaatattc atcaaaggtc aagtaacaat aaaagcagga actaacatgg aaagaccagg
    14881 acctttagct gctcaccgtg catttgacta tgtaactcaa gaaataagag aagcattaaa
    14941 gaaagttgac aactctttag tagttgatga agaagtaata gagcaataca gaagagaagg
    15001 taaaaagaaa gttgttgtta taaaagaaat aatgggacaa ggtgcaatgc atgataacct
    15061 aatattacca gttgagccag ttggtacatt aggagctcaa ccaaacgttg acttaggaaa
    15121 catgccagtt gtattatctc cacttgaagt attagatggt ggtatccatg cattaacttg
    15181 tataggacct gcatcaaaag aaatgtcaag acattactgg agagagccat tagtaataag
    15241 agctatggaa gacgaagaaa tagatttagt aggtgttgta tttgttggtt ctccacaagt
    15301 aaatgctgag aaattctatg tatctaagag attaggtatg ttagttgaag ctatggaagt
    15361 tgatggagct gtagtaacta ctgaaggttt cggaaacaac catatagatt tcgcatctca
    15421 catagagcaa ataggtatga gaggtatacc agtagttggt gtaagtttct cagctgttca
    15481 aggtgctcta gttgttggta ataaatacat gactcacatg gtagacaaca ataagtctaa
    15541 gcaaggtata gagaatgaaa tattatctaa caacacttta gctccagaag atgctgttag
    15601 aataatggct atgcttaaaa atgctataga aggtgtagaa gttaaagctc ctgaaagaaa
    15661 atggaatcca aatgttaaat taaataacat agaagctata gaaaaagtta caggagaaaa
    15721 aatagtatta gaagagaatg agcaatctct accaatgagt aagaagagaa gagaaatata
    15781 cgaaaaagac gaaaactaaa atttagatac taactaagat ttagacaata acataaattt
    15841 agatccaagt ttaaaacagt atagagaggt agaattataa tggataggtt aaaatatatc
    15901 tcatcagaga ctttttatga aggtattata gttgacatca aaggcggtgg agtgactata
    15961 gacctaaaag gtagacttgg acaatttaaa atacctaata gaatgcttat aactgactac
    16021 gaacttaaga taggtcaaga agttggattc atgctaagct atcctgaggt attaagtcca
    16081 gagcctaatc aagagtatgt agaaaatatt aggagagaaa aagaaaaaca agcagaaatg
    16141 caatcaaaac aagaagaaaa ataataaata atcgtttgaa aatagaaggg agaggaaata
    16201 tatgagcctt acaacagtac aaggacttca atctgaaata ttcgttccaa taacacctcc
    16261 tccagtttgg actcctgtaa ctaaagaatt aaaagatatg actatagctt tagctacagc
    16321 agctggtgtt catttaaaat ctgacaagag atttaatcta gcaggagata ctacatttag
    16381 agctatacct aatacagcta cagttgatga aatgatggta tcacatggtg gatatgacaa
    16441 tggagatgta aacaaagata taaactgtat gttccctata gatagattac atgaattagc
    16501 tgcagaagga tttatcaaag gagtagctcc tatgcactat gcattcatgg gtggtggagg
    16561 aaaccaacat gtcttcactg aagaaactgg tcctgctatc gctgctaaac ttaaggaaga
    16621 gggagtagac ggtgtagtta tgacagctgg ctgaggtact tgccatagaa ctgccgtgat
    16681 cgtgcagaga gcaatagagg aagctggaat acctacaata ataatagcag ctcttcctcc
    16741 agtagttaga caaaacggaa ctcctagagc agttgctcca ttagttccaa tgggtgctaa
    16801 tgctggtgaa ccacataata tagaaatgca aacacatata ttaagagata cattagagca
    16861 attagttgca ataccatctg ctggtaagat agttccatta ccatacgaat ataaagctca
    16921 cgtttaaaat gtaatttata tttgaaaata tagaaccctt ctcctacacg gttaggagag
    16981 gggttttaag aacaaaggtg gatattatgg aagaaaaaat aatgagaaga cttgttataa
    17041 aaccgtttca tatgaatgaa gttaatttcg gttcaaaaac ttctataaag aaagatgtac
    17101 ttacaataga tttatcaagt atagatgaaa taaaagaaag agaagagtta ataacagaca
    17161 taaaagtaga cattataaaa cctggagatt atgacagaga aattaatact attatggaca
    17221 ttataccaat atcaacaaaa gttttaggca gactgggaga gggtattaca catactttaa
    17281 cgggtgtata tgtaatgtta actggtgcag atgaagatgg aaatcaaatg catgaatttg
    17341 gttcttcaga aggaattttg aaagaccaaa tggtatttgg gagatatgga actccatcag
    17401 ttgaagatta tataatccat gtagatgtaa ctttaaaggg aggtttacct tttgaaagaa
    17461 cacttccact tgcagcattc agagcatgtg atgattttat acaagaaata agagcgtctt
    17521 tgaaaatgga agacggaaga aatgctactc aagttcgtga atattttgac aaaattcgac
    17581 caaatgcaaa aaaggttgtc atagtaaaac aaatagcagg tcaaggtgct atgtatgaca
    17641 atcagttatt ctcaaaggaa cctagtggat ttgaaggagg aacatcaatt atagatatgg
    17701 gaaatgtacc tatgattata tctcctaatg aatacagaga tggtgccctt agagctatga
    17761 cttaagagag gtggatatta tgggaatagg accttcaaca aaagaaacat ctcttcatca
    17821 ctttagagac ccacttttag acatagttga aagtgataag gatgtagatt tacttggagt
    17881 aatagtagta ggaactccag atggaaatga aaataaaacc tttgtgggac aaagaacggc
    17941 cgcttggctg gaagccatga gagtagatgg agctatagtt tcatcagatg gttggggaaa
    18001 ctcacatgtt gactacgcta acacatttga ggaaatagga aaaagagata tacctgtagt
    18061 tggagttaca tttaatggga ctcaagctaa gtttgttgta agcaatcaat atatggatac
    18121 catagttgat atgaacaaat caaaagaagg tattgagaca gaagtagttg gagaaaacaa
    18181 cacaaatgaa atagatgcta aaaaagcttt ggcattttta aaacttaaaa tgagaaaaca
    18241 tggataaaaa atttaagtaa gggaatgagt tttgtgggaa tttctacatc aatgaaaaac
    18301 acatcacttc atatatttag agacccgtta ctagatttga tagataatga ttctgacata
    18361 gacttgttag gagtggttgt agttgggaca tcagagcaca atgaatggaa aacattttta
    18421 gcaactagac ttggaagatg gattgaatca ttgcgacctg atggagtgat tataacttta
    18481 gatatggcag gaaaccagca tattgatttt acaaatgcta tagcagagtt tgtaaaatca
    18541 gatataccta cagtaggtct tactattatg ggagcagatg gattagtaat taccaatcca
    18601 tatttggata aagctaccat aattgattat aaaaagacaa caggctacat agaaacagaa
    18661 gtagttggag acaatcacat ggatgaagtt gatgttaaaa aagcattggc tttcctaaaa
    18721 ttaaaaatgc gtaaagacgc aaaataaacc taagggggaa tttgatatga aatttagcag
    18781 aagtatacaa gctatagact ctcatacagc aggtgaagcg actagaatag ttgtaggtgg
    18841 aatacctaac ataaaaggaa attctatgcc tgagaaaaaa gaatatttag aggaaaatct
    18901 agattattta agaactgcta taatgttaga gccaagagga cataatgata tgtttggttc
    18961 tgtaatgact caaccttgtt gcccagatgc tgactttgga ataatcttca tggatggtgg
    19021 cggatacctt aacatgtgtg gccatggtac aataggagct atgacagcag ctatagaaac
    19081 aggtgtagtt ccagcagtag agcctgtaac tcatgtagtt atggaagctc cagcaggaat
    19141 aataagagga gacgttacag ttgtagatgg aaaagctaaa gaagtatcat tcttaaacgt
    19201 accagctttc ttatataaag aaggtgttga agttgactta ccaggtgttg gaactgttaa
    19261 atttgacata tcatttggtg gaagcttctt tgctataata catgcaagtc aattaggttt
    19321 aaaaatcgaa cctcaaaatg ctggtaaatt aactgaacta gctatgaaac ttagagatat
    19381 aataaatgag aaaatagaaa tacaacatcc aactttagct catataaaaa ctgtagactt
    19441 agttgaaata tatgatgagc caactcatcc agaagctact tacaaaaatg tagttatatt
    19501 tggtcaaggt caagttgaca gatctccatg tggaactgga acaagtgcta aattagctac
    19561 tttacatgct aaaggtgaat taaaagtagg agaaaaattc gtatatgaaa gtatattagg
    19621 aactttattc aaaggtgaaa tagttgaaga aactaaagtt gcagatttca atgctgtagt
    19681 acctaaaata actggttctg cttatataac tggatttaat cattttgtaa tagatgaaga
    19741 agacccactt aaacatggat ttattcttaa ataagtaata aataaattag gaaattaaaa
    19801 tagaatagta gccacttgtc ggttgctatt ctatagcttt taggctataa gattcagaat
    19861 atatgaaaaa ataaaaaaat aggaggatgg attatggtag caatcttaaa ggcattatat
    19921 gtagtattcg cagcaatctt tgcagttgtg tttggtaaag acgtagctaa agcaaacaaa
    19981 gaggggaaac tagaagaaca aagtactcca aaagttgctg taacaggatt tataacagac
    20041 ttctttgata cattaggaat agggtcattt gctccaacaa tagcaatgtc aaaggcttta
    20101 aagttaaata taccagataa aaaaatgcct ggtacattaa acgtagcaca tactattcca
    20161 gttgttacag aagcattcat atttacatca attatcccag tagatggtgt tactttagta
    20221 tctatggttg tggcagcagc agttggttca tatataggtg caggaataat agctaagatg
    20281 gacgagagaa aaatacaact tgttatgggt gtaactttag cattaacagc aatattaatg
    20341 ttattaggtc atccttggat taacgtatta ccaggtggag gaaatgaatt aggtcttact
    20401 ggtgctaaat tagtaatagg tgtaataggt aactttatac taggagcttt aatgacagct
    20461 ggtataggtc tatatgctcc aggtatggca atggtttatt tcttaggaat gtctgcaaaa
    20521 gttgcattcc caatagttat gggttcttgt gcattattaa tgccagttgc aagtatgaaa
    20581 ttcataaaag aagatgcata tactaaaaaa gcatcagtta taatagctat atgtggttta
    20641 ataggagttt tcgtagctgc atatatagta aaaactctac caatggatat attaaaagca
    20701 ttagtaatag tagttatagc ttatacttct gctacaatga taatggctgc aaataaaaac
    20761 aaaaagactg cttagttaaa gactacttag tgtttattaa atattacagc taataataaa
    20821 gattataaac tgctttaaaa tattgaatta atattttagg gcagtttttt atttactaaa
    20881 ttaatataaa gcaattaaca tgccaattat gtaatatata tagtgattta gtagatttaa
    20941 tctaaaaaac gttgtatttt taaagaataa agcataattt aaagctaaat atttaaattt
    21001 tataatcaat aagttcaata aatgttcata tattaataca gattaataaa aatataacct
    21061 gtaataagtc taatataact tataattaag aaaaataagt ttataatgac tcaatattca
    21121 tctgaattat ttcaataaat ttaattcgtt gaagttgc
//
LOCUS       DAMJZE010000052        21110 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJZE010000052 DAMJZE010000000
VERSION     DAMJZE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.052"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..139
                     /locus_tag="PC541_001719"
     CDS             <1..139
                     /locus_tag="PC541_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2839186.1"
                     /translation="QTQNLYVVGGVACEKIGSMTKEKFTMIKGNDRFDTLYKALEFIN
                     K"
     gene            389..880
                     /locus_tag="PC541_001720"
     CDS             389..880
                     /locus_tag="PC541_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839187.1"
                     /translation="MAYENGTNLVAYFPNCIKKYEKDNKIFEKTILLLNLYMQMPTWP
                     HCIHEFKAKANEILGEDPEFIGEYGIKLKDFYNEGENVNISQNFKNDLYTFSNSTYDI
                     LDNSYIQRINPIGLYSISSLARENDFILKLFRNDKKSLELELSKSEIQSIIEHLESMI
                     EEE"
     gene            883..1467
                     /locus_tag="PC541_001721"
     CDS             883..1467
                     /locus_tag="PC541_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839188.1"
                     /translation="MKGEYNKEKEKVIKFNSNKDKSKINKYTDYYNTLNSTLMEKNKC
                     IGDNWFLATTSEEVGNMNEEEKRILETEKRILETEKRVHQNNKEVKESIDKQFNDIKG
                     IFKEYKNDLDKDRIELKNDLARERIELKSDLKEAINEHKKVTEKDVSEIKNSIKGIED
                     RIDSTNKWIIALCITTIIGIATMAITIGISIWPK"
     gene            complement(1877..2065)
                     /locus_tag="PC541_001722"
     CDS             complement(1877..2065)
                     /locus_tag="PC541_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839189.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            2318..2467
                     /locus_tag="PC541_001723"
     CDS             2318..2467
                     /locus_tag="PC541_001723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839190.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
     gene            3003..3392
                     /locus_tag="PC541_001724"
     CDS             3003..3392
                     /locus_tag="PC541_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2839191.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            3486..3869
                     /locus_tag="PC541_001725"
     CDS             3486..3869
                     /locus_tag="PC541_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2839192.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            4548..5978
                     /locus_tag="PC541_001726"
     CDS             4548..5978
                     /locus_tag="PC541_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2839193.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFLLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(6126..6476)
                     /locus_tag="PC541_001727"
                     /pseudo
     CDS             complement(6126..6476)
                     /locus_tag="PC541_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            6471..6992
                     /locus_tag="PC541_001728"
     CDS             6471..6992
                     /locus_tag="PC541_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2839194.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(7121..7357)
                     /locus_tag="PC541_001729"
     CDS             complement(7121..7357)
                     /locus_tag="PC541_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839195.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(7667..11095)
                     /gene="uvrA"
                     /locus_tag="PC541_001730"
     CDS             complement(7667..11095)
                     /gene="uvrA"
                     /locus_tag="PC541_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2839196.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            11414..13648
                     /locus_tag="PC541_001731"
     CDS             11414..13648
                     /locus_tag="PC541_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2839197.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            13904..15934
                     /locus_tag="PC541_001732"
     CDS             13904..15934
                     /locus_tag="PC541_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2839198.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            15931..16383
                     /locus_tag="PC541_001733"
     CDS             15931..16383
                     /locus_tag="PC541_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2839199.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            16385..17440
                     /locus_tag="PC541_001734"
     CDS             16385..17440
                     /locus_tag="PC541_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2839200.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            17507..17815
                     /locus_tag="PC541_001735"
     CDS             17507..17815
                     /locus_tag="PC541_001735"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2839201.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            17919..19184
                     /locus_tag="PC541_001736"
     CDS             17919..19184
                     /locus_tag="PC541_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2839202.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            19272..19988
                     /locus_tag="PC541_001737"
     CDS             19272..19988
                     /locus_tag="PC541_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2839203.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(20183..20476)
                     /locus_tag="PC541_001738"
     CDS             complement(20183..20476)
                     /locus_tag="PC541_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2839204.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(20482..20697)
                     /locus_tag="PC541_001739"
     CDS             complement(20482..20697)
                     /locus_tag="PC541_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839205.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         8019 a         2376 c         3361 g         7354 t
ORIGIN      
        1 tcagacgcaa aatctttatg ttgtaggcgg tgtagcatgt gagaagattg gttctatgac
       61 taaagaaaaa tttactatga taaagggtaa tgatagattt gatacacttt ataaagcact
      121 ggagtttata aataaataaa atcaatagag ttaatatatt ttacattttg actacaattt
      181 tatgacagta tcacaacaat ttataaaata aaaaatgata tattaaatat aaccataagt
      241 catctacaaa ctttaaaaaa tcaggtatat gaagagcagt tgagtatata gcaataagtg
      301 gtaggaggag gaataaaaaa taatagtgca atgaaagtaa ttataatgta gaatagtatt
      361 aaataatatt attttagggg gagttaagat ggcttatgaa aatgggacta atttggtagc
      421 gtattttcct aactgtataa aaaaatacga aaaggataac aaaatatttg aaaagacaat
      481 actattactt aatttatata tgcagatgcc aacttggcca cattgtattc atgaatttaa
      541 agctaaagca aatgaaattt tgggagaaga cccagaattt ataggtgagt atggaattaa
      601 actgaaagac ttttataatg aaggtgaaaa tgtaaatata agccaaaact ttaaaaatga
      661 tttgtatact ttttcaaata gtacatatga tatattagat aactcatata ttcagagaat
      721 caatccaatt ggcttatatt ctatatcttc tttggctaga gaaaatgatt ttattttgaa
      781 actatttaga aatgataaaa aatctttgga attagaacta tctaaatcag aaatacaatc
      841 aataattgaa catttagagt ctatgataga ggaagaatag ttatgaaggg tgaatataat
      901 aaagaaaaag aaaaagtaat aaagtttaat tccaacaagg ataaaagtaa aatcaacaaa
      961 tatacagact attataatac tttaaatagt actttgatgg agaaaaataa atgtattgga
     1021 gataattggt ttttagctac tacttcagag gaggtaggta atatgaatga agaagaaaaa
     1081 agaatactag aaactgaaaa aagaatatta gagactgaaa aaagagtaca tcaaaataat
     1141 aaggaagtaa aagaaagtat agataagcaa tttaatgaca taaagggtat ttttaaagaa
     1201 tacaagaatg atttagacaa agatagaata gaactaaaaa atgatttagc tagagaaaga
     1261 attgagttaa aaagcgattt aaaagaagct ataaacgaac ataaaaaggt gacagaaaag
     1321 gatgtaagtg aaataaagaa tagtataaaa ggtatagaag atagaattga ttcaactaat
     1381 aaatggatta ttgcattatg tataactact ataataggaa ttgctacaat ggctattaca
     1441 ataggtatat ctatatggcc aaagtaacac aaaaagaggt aactagaatt aaaatagtta
     1501 tctcttttta tacttaaaca aaacattgaa aaaatagtaa aaaatattaa caggatacat
     1561 aaaactatag tataacctgt aataataaaa cgaacgaaaa ttatcatact aaaacataag
     1621 acatgctata attgtattag ataaatgctt gaatatatac caaaagtact ctttttataa
     1681 gagtgcttat ttttttgaaa ttcatcaaca tataaactat caagaacatt actcaacaca
     1741 ccttaaaatt gatttaaatt ctttttcata cacaaagtta tatgatataa taaaaaagta
     1801 aatatgtaac cccaacacat ctttactaag tcaaacatta ttatatagag cattcttcat
     1861 tatggagagt gctttttcat ttctttgaat aatcatgttg attatttata atattttcat
     1921 ttaatttatc tttatctatc aaatttaatg cacaattaat acaaatgtta gttttcatat
     1981 gacttttctt atgaaaagta atatacttat tatcatcttt atttattcct ttattacagt
     2041 aatcacataa tatagtctta gtcatatttt tattcctttc attttatatt ttccataatt
     2101 aagtttaaca tatttagtat atacctatgt atatatattt aaaacaatgt tttttattgc
     2161 aatatagaat atattgtata aataaaaaaa tatggagaat ggatatttga taattgtaaa
     2221 attatgttat aatttatatg taaggaacta aatatctaag agtggcagtt tccacattat
     2281 gctctaacct actttctaat gaaaggaggt gggaagtgtg gaaatagtag cagtttatta
     2341 catagcaaaa tttttacttg atttatacaa agtaaaaagc cactcaaagt cggaagctaa
     2401 gagtggcttt atatttaatt taactattaa taaactaata gtaaaaatta aatataaaaa
     2461 gtactaaatt ttttcggaaa ctacactctc gacaatagat tatagttcct tatttctaaa
     2521 gaggtaggag aattgcagtt ctcttatctc tttttgatac gaatttacat ctttattata
     2581 tcgcattttg agaaaaaata aaactatgaa tattagaaat atattttatt ttatctctaa
     2641 gtatatatat tattagccac ctaaaaaact ttcacaaaaa caaaaagagt atatgttttt
     2701 gataactgga tgatataact tataactcat tttacaattc ttagttcata tatatttatg
     2761 ccactaaatt tctctagttt tgccgattta gttttattct acaactaaaa taatacaata
     2821 gaattagtta ttattcaaca aaattatttg aaaatttaat aattttatag atatgaaagt
     2881 tattttcttc taagaaaaca gactaaatgc gtagaatatt tggtaaaact tacctgtata
     2941 aattgaaatc agaaggtttt attattgatt tagattagaa caatttgagg gagtgacata
     3001 aaatgaaaca acaagaagta gttaataaac tacgcagggg agaattgatg ttgatggaat
     3061 acttgtggag gaaaaattct attttatcta aaaaagaaat aatagaagca atgaaagaaa
     3121 aatataagtg gaggaaaagt actacagaaa ttctattaaa aagattagta aaaatgaaaa
     3181 cattaaaaaa gaagaggata ggttttcagt ttagttatga agtgctagta actaaaaaag
     3241 aatatttaaa tgcaataaaa gaagatagaa aggtaactaa atatgatagt ttttttatac
     3301 gaatgtttac cactatgcat aaaaaagaaa agatgacaga agaacaaata aaaaaatata
     3361 tagaaaatat ggaaaaaatt ggaaaagaat aatttatata tatattaatt atttttaaaa
     3421 taaatttgtt ataatattta aatatacata aacatacata aatttacaaa aagggggtgt
     3481 agcttatggg attcaagaaa ttgccagatt ctgagttaaa agtaatgaag tttatttggg
     3541 gattagatgg aaataaggta gcatcaggag aagttgtgga agcaatgaaa caaatttatg
     3601 actggaataa aaatacgaca ttaacaatat tgtctaagct tgcaaagaaa aactatttgt
     3661 attctcaaaa aacaagcaag tgtacatatt atactatcgc tgtaagagaa aaagattatt
     3721 taaaagttga aacaaaaaaa ttctttagtt tttttcataa taattcttta aaaagttttt
     3781 tcactgcttt aaatgatgaa gaaaatttaa gtgatgataa attagatatg ttagaggaat
     3841 gggtgaaaaa ttgggaagaa gatgaataaa atgaatgtga tactaaaatg atactaatat
     3901 gttttatagc gtgttaaaaa atatagaaaa tatagaaata atagaagaaa attaatgtaa
     3961 tagataaggt tttatcagat taaaaagaaa atcaatagaa tgggaataaa atatagaatg
     4021 ccaaaaatcc acataaatac aggatttaag gtctctctta cttctatgtt atactggttt
     4081 gatactatca atatttgcca gtatcttatg gattaataag taaaaaacat cctgctgaat
     4141 gataaattca gcggggtgtt tttattttgt tttagaactc ctttgctctg aaactcgaag
     4201 tatttaacac tgatgatttt gttatctttt agttcctttt gtgtcatgct ctgaaagaca
     4261 aattgataca ataaaaaagt aatttcttaa tcattgatgg agatatgtat ttatgttact
     4321 cttgatggta atttgagtaa tattgttcat agctttttat attcaattaa ttagaaaacc
     4381 atttggtata atcataaata aacttgatga tataatagaa aagatttata attattagaa
     4441 gtatataaaa gtattttatg tctatgaaat gtatgtctat aaaacatata gatgactttt
     4501 gtatattaat gtatgaatat tgatattata gaagaattga gttgtcaatg atactaaatc
     4561 aaatatttat agaagctgga gggattatga gaaataaaat aattatagga attacttttt
     4621 tacttgtatt actaatgtct ggatgtgctt caaaacaaac cgcagaaata aacgtattag
     4681 caactactga tttacatgga gttatacctt atgagttaac ttcttatgta aaagaagaaa
     4741 gaaaaaaaga taaaaatcta actttagtag atgcaggaga tttttttgat agtggagaag
     4801 tgtttggttc tagcatggat agttatttta gtgctagata taaagatggt gataataaca
     4861 ctgaaagtta tattgagacg cctatttcaa aagatatgaa agaggtaggt tatgatgttg
     4921 cagttcttgg gaatcatgaa tttatatcca acaataaatt ttatctagat aatatgatat
     4981 ctgattttga gaagcaaaaa ataaatattt tatcggcaaa tacgtataaa aaaaatggag
     5041 acagttatac aaaaccatac ataataaaaa atattaatac acctgaagga aacgtaaaac
     5101 taggaatttt agggcttact ataaaagaag ttggtgagag gaaacagtgg aaagatggaa
     5161 aacttgttga ctctaagtct ttagaactaa aagaccaaga gggctacaat ggtgaattgt
     5221 atatgaatga tttagtagaa gatgctaaaa agtgggttag tataatggaa aaagagaagc
     5281 ctgacattat agttgctgtg gcacatacag gtgagaaacc taagaaacct aaaaacccag
     5341 gaaatagaat acaagactta gctcaacaag ttgatggaat tgatgctata gttgcaggtc
     5401 ataatcatgt tcaaattgag caacatgatt ataaaaataa atcaggagaa aatgttatag
     5461 ttactgagcc aggaaagcat ggggaatgta tttctaagat taattttaaa ttagaaaaaa
     5521 acaatgataa atggaatata gttgacaaat ctagtaaatt aattaaattt gaaaaaaatc
     5581 aagaaagtga taaatttgga gaatttattt cttcaattaa taaagtaagt aaagataaga
     5641 aagaaataag attatcagaa gtaaagactc ctgaatggga taaaatatat gtttttaaat
     5701 ctggaacatc tgcagatgat atatataaaa caataggata taaatggaga aatatatcat
     5761 ctccaaagag tgatggcatg gtgcagattg ttgtaatgaa taataataag cctgttagct
     5821 atatatatgg tgaagctgaa gttatgccaa ttaatataaa atgtgatgaa tcagagtata
     5881 aagataatgt tgtaactatt tatcctaata aaaatgataa atttaaaatt gaaaaaggta
     5941 aacaagaata tagtattaat ttaaaacata tacaatagaa acttttatct tatttaagtt
     6001 tagattgtac ttaaaataaa gtgtgtatta ttttaaaaat tgatgataat taaaatcaca
     6061 acattaagga atagtacaca ctttttatgc taaaaaatag aatgttaaaa aattaaacct
     6121 ttaaattaaa gtacattgct tatattatcc aatattatct tcaaaggttg tgtaatattt
     6181 ttatccttat gtaatattgc taacatttta ttttccatat ctatcccatc taattcaatc
     6241 aatcgcaatt ttttatgtaa gagtgaatct aataataaat tttctggcaa aatggttata
     6301 cctaaccctg cttgtgcagc tttaataagt gcaaaagaat taacactttc ccaaacagga
     6361 taagcttttt ggtttgaaag agataataca ctgtccaaag tatcgcgaat ggcacttcct
     6421 tgctcacgta acaataacgg aaacgtgcat aactgtcgta gagataaggc ttgtttagta
     6481 ttggtggtgg ttatgcaatc atacctttaa tccaagagaa agtagtaaat caaactggat
     6541 ggataagtga gaaaatattt acagatatta taactatttc acaaatgaca ccagggccac
     6601 tggctgttaa tacttctact tttgttggac ttcaaattgg agggttttgg ggagcagttg
     6661 cagccacttt aggttgtgta ttatgtggtg tagtaatatc tctattttta tataattttt
     6721 ttcaaaagca tcagaactct acttatatat ttgaaatatt aaatggtcta aaatcagctt
     6781 cattaggttt aattatctca gctgcggcta caattataat tcttgcatta tttgggacga
     6841 ataaagtgta cttcaattct atatttgaga tgctaaactg gactgcacta ataatctttt
     6901 ttgttacact actgatagta cgtaagtgga aaattaatcc tattgtgatt ataataatga
     6961 gtggtatact gggaatattt ttttatttat aagtttttgc taagtaaagg atatctattt
     7021 ttttatatct taaatccaaa tagccaataa agtaaggcta tttggatttt taataaattc
     7081 tgaaaagata atcagaaacc tatataacat ttaaattata tcactggttt tctacaaacc
     7141 aatcataagc ttcttggcaa aatttcttac tatctatata gtaactatta tgaacattat
     7201 taatttcaac aagtttcttt ttacatttta gtgaattata atagctatca aaatattggg
     7261 ttttaaaata tctatcatga agtgttttaa agacaagagt aggtatgttg actccatctt
     7321 ttatcttact atcttttgca ataaagcatt ctaacattgt attaattatc tttgtactat
     7381 ttttccaaat atgaggattt ttcttttcaa atattttata aaattcatta tcatagactt
     7441 cattataacc tttatattta ggtggtttta ctgttatgtg aaaattagga agaaacttaa
     7501 agaaaaaata taataatttt tatgttctgt tttatatatt attcatattt tatcacagat
     7561 aatatactaa agttaatttt tttattgtac aactattata ataattcttc tataatataa
     7621 gacattctat aattttatat ttaagttcat gtgtatttaa ttgtttttat ataaaatgtc
     7681 cagtaatact atttggattt atttttattt catcaggagt tccagaagca acaatttccc
     7741 ctccatttat tccactgtct ggacctatat cgataatcca atcagaagca cgtataacct
     7801 ctgtattgtg ttcaataatt aaaagtgaat gctgttcatt tacaagtttt ctaaaagtaa
     7861 ttaacaaatt ttctatatct ttaaaatgaa gtccagtagt aggctcatcc aatatgtaca
     7921 gcattcgttt tttagttttt gtccctaatt cttttgctaa ttttatcctt tgagattctc
     7981 ctccacttaa tcctaatgta ttttgtccaa gctttagata tccaagtccc acttcatcta
     8041 ataaggagag ttttctatat atatcagagc aatcgctaaa aaatgttttt gcttctgcta
     8101 cttccatgtc aagtatatct gcaattgtct ttcccttgta ttgtatagac agtatttctt
     8161 tttgatatct cttaccttga cattcactac aagttaccca ttggtctgcc atatattgca
     8221 gaataatttt tgttttacct aatccttgac agtttgggca ttgccccttt ttgctattaa
     8281 agctaaaata gctttcatcc aataattttt gttttgcttg tggagtttca gcaaatagag
     8341 aacgaatttc atcaaaaata ttgatatatg tagcaggtgt agaacggcta gattttccaa
     8401 taggtgcttg gtccattaag ataaaatcat caatagcatc aaatccagta aattcagtat
     8461 aatttttatc tgggatagat ttttgtttca atttttcttc tagagcagga agtaaactgt
     8521 gaaatacaag agagctctta ccagaaccac taacacctgt aatagagcac atacagttta
     8581 agggaatttc tacatcaata ttttttaagt tatttgcatg gcatcctttt aagcttaacc
     8641 atttacttgg tataatttta ttttgtgaac ttattttatt gtatgtactt aaatatttac
     8701 ctgttataga gtttggattt tttataattt cttgggttgt accttctgcg ataatgaacc
     8761 ccccttttgt accagcactt gggcctacat caataatata atcagcagac agtattgtat
     8821 ctttatcgtg ttccacaact ataacagtat tacccttatc tcttagctct tctatcattt
     8881 tgatgattaa attatggtct cttgggtgta aaccaataga tggttcatct aaaatgtagg
     8941 ttaagcctac taattcactt cctagctgac ttgataatcg tactctctgc agttctcctc
     9001 cagataaagt tggagctgtt ctatctgttg atatatagct aagacctacc tttaatagat
     9061 aactaactcg aatttttaat tctgttaaga tatcatttac caaagagagc tcattttttt
     9121 gaaggtgatt tggtaattca cacaaccaat tccataactg ttctatcgtc atttttgtta
     9181 gttcagggaa gcgataacct ttgattgtag tgaatctagc ctcaatgcac agcaattcgc
     9241 caccacatgt attgcaagga attttgttca tatattgatg caaggtatta ttttcagaac
     9301 tagattctct aaaaagtctt tgaatgtggt tgattgctcc tgaagcaggt cttctaatct
     9361 ttgattctcg tccctttgat tcaaagctaa attcatatat cttatcgtct gtaccatata
     9421 agattgcatc tataaaatct cttggtaatt cattccaagg tatatctagg tctatatttt
     9481 tatcttttgc tattgcatat aattcaccta ccatccaatt accatttggc tttttccccc
     9541 tcaattttcc aaaatagtaa gtagctccat ctaatattga aatagttggg tcttttacaa
     9601 ttaattgagg attaacttga taggtaaaac ctaatccatt acaatcgtga caagctccag
     9661 tatgggagtt agcattgaag gttgaagtat ttatcttaga aaaaatagtt tgacaatgtg
     9721 gacaataatt ttttgtatta ttcttttttt ctaatggaat tttgcaatat ggacagtaac
     9781 tttttccgat tgatacaaat aacattctta aataatccca aatgcttgta agagtaccaa
     9841 cagtagagcg aggattttgg cttccttttt tctgttcgat ggctatagta ggggttaatc
     9901 cctgaagtaa atcaaattcc ggtttttgaa gctgttctga aagttttgct ccgttactta
     9961 gattttcaaa ataacgtctt tttccttctt cataaatcgt atcgaaagct aaagaagatt
    10021 ttccactacc acttacacct gtaataacag ttatttgatt ttttggaatt gtaacttcaa
    10081 catcctttaa attgttttgc cttcctcctt ttaaatagat agagctttta ttatgacaaa
    10141 gtgaattttt aattgattta tttattgctt ttgtaaattt gaatttcttt tctagttcaa
    10201 gaggattgcc taattctttt atttccgaaa tacctaatat ttttaactga gaaaagattg
    10261 atttcaaata atcttcaggc aatttttcta ttaacaaatt aagggcttct atttttatat
    10321 ttccgcttga taatatctct gccatttcat ctaataaatt tttttctgct cttgttaatt
    10381 gattgtattt tttggaactt aattctgtcc ataaatccca tggaagcaat tctaaaccaa
    10441 tcatattatt aaagtcatgt agaagattac cctttacaga atgccaacct ttccaatttt
    10501 tattatatcc aaagtcatct ggatgagcat gtccttgccg acatcttatc caggcttctg
    10561 aaccagttaa aaaaccatct gtttttccga catcatgagt gtcaaaatta atttgaaaat
    10621 aatttttttg tatgtcatct atttgtgcat ctattcttat ccatctcttt gttagagtat
    10681 catagtattc tacaagccag tggtcttcat aagtaagctc actttcaaaa taattagcaa
    10741 aacctgctct cattctagcc tcaaaacctc gatatcttag gagcgataca agaagtaaag
    10801 aataatctct gcacattcca acaagccttt gttttgggtt tcttggaata gttagtgggt
    10861 tagaatttat ttcaaatatt ctttttaaca tttgaggaat tgtacgaagc aattcctcat
    10921 cagattgttg cttattaaag gatataccat ataattttcc ttggtctcca tgaattacta
    10981 ggctttgaac cacttttaat aactcaattg gagtctttgg taaatcttga tatttatttt
    11041 caaatttttt aggatttgtg atgatacttt gatttttata gttatttatt ttcatttata
    11101 tttcacctct ctaaatggat gatataccct gacatggtgt catatgcaag gacaatttag
    11161 atttttggcc aaaatttaaa aaataaataa aaaatgatac agtgataaat tgtaatacaa
    11221 atatagtttc aaattgcata taatataatt atttaataaa accattatct attgaatgtt
    11281 aatgaaatgt taagatggca ttcttgaata ttaagaaata gaatactata atatagtgtg
    11341 aaggattttt agtaaaaaaa tttgtaagtg ttaatataca ttaaaaaata ggtttataat
    11401 ttggaggaaa aaaatgaaat tatcaaggta taagaaaaat agtttagttc tgtctataat
    11461 tttatgttca cttattattt caatccttgt aattactgtt tttaataccg taaaactaca
    11521 caatatatct aataaacaat ctaaaaacta tttaaatgat gtatctacac agattgtaat
    11581 gaatgttgat tctaaaatca aatttatttt atccgactta agaataatgg cagattttat
    11641 taagcaatat gaaggtgata gtcgatatga gtatttatct aaaagaaaga ctagttataa
    11701 gtattatgat gttggaataa tagatttaga aggtaaagct gagtttttaa gtggaaaaaa
    11761 gtttgactta aagaatactg tttcctataa aaaaggaatt gaaggtaaag aatactcaga
    11821 agtaatagag tcaatgggtt ttgttcttta tagtgtacct atatttaatg ataatcatga
    11881 agtatctagt attttaatag gtgtatccaa taaagaaagt atgaatgaga ttcttaatat
    11941 agataacttt aatggaaaag gaactattga aattattaat agtaagggaa aacctctttt
    12001 cataggaaaa aatactgaat taataaagga ccttagccat aaatataaga gttctggaga
    12061 tgaaccttgg gcacaaaaga tgcttgagga ttttaaaaat gataaatcag ggaatattac
    12121 aataacctca tcaaaaggtg tacaatgctt attaacttat catccaatta caagaggttt
    12181 aaatgattgg cattttcttc tcatagtacc tgaagatgct gtacttggtg aacttaataa
    12241 attaaactct tttacaataa caatgacatt tataattaca tgtattattg gaattataac
    12301 attaatttta tatattataa gaagaaaata tgtaaatcaa atagaaaata ttgcatattt
    12361 agatagtata acaaatggta taaattcaac taagtttagt atgctagtca aacctttaat
    12421 ttcagaatca cctgatagta cttatatgat gatagcaatg aatataaaag attttaagct
    12481 aataaatgat tgtttcggta gtgaaaaggg aaatatgaca ctaaaacatc tttacaatat
    12541 tttgtataaa aacattgatg aaaatgaaga atttgtgtgc cgtcatgatg cagacctttt
    12601 ctatttgctt attaagaata gaccagtgtt agaagccctg gagttattat ataaaataga
    12661 agctgacaca aattatttta ataaaaatag ggaaaatcca tactttttaa gactatcagt
    12721 aggtatttac acaattgaaa atcataatga agatttaatt acaatacaag accatgcaaa
    12781 tacagcaaga aaaagcttta ataaaactca tcaaagtgat ttttcttttt atagtgatat
    12841 agaaagaaaa cgattaatat ctgaaaagga aatttcaaat ttaatggaaa aagcccttga
    12901 aaataaagaa ttttttatgt gcttacaacc taaaattgat ataaaaagtg gaaaaattag
    12961 tggagcagag tctcttgtgc gttggaaaaa ttcagaaaag ggcataattt atccaagtga
    13021 ttttattcct cttttcgaaa aaagtggatt tatatgtaag ttagatttat atatactaga
    13081 agaaacatgc aaattgattt ctaagtggat aaaagaaggt aaagaagtgt tgagaatttc
    13141 tgttaatgta tcacgtcaac atctgaatga taaattgttt ttagaaaaat ataagcatat
    13201 atgcaataaa tacaatgtac caacatgtct gatagattta gaacttaccg aatctatttt
    13261 cttagaaaat ccagaagcta tagatataat agaggatatt cattctaatg gatttaaatg
    13321 ttctatagac gattttggat ttgggtactc atcattagga atattaaagg attttaaagt
    13381 tgacataatt aagttagacc gttcattctt tgttagcaaa aataatatag accgtggtaa
    13441 ggtagttatt aagtctatta ttgaacttag caaaaggcta ggtatgaaag taactgctga
    13501 aggaattgaa gaattagaac aagttgagtt tctaaaaaat gtgggatgtg attatattca
    13561 aggttatgtt ttttcaaaac cattattaat ttctgatttt gaaaattttg catatgaaaa
    13621 taataaaatt aaaaatatcc attattaaat gtacaagata aaaaatttag gcaagttata
    13681 taattaaaaa tataacttgt cttttttatt tatatattta ataaaaatat atttgcagat
    13741 tttgtaaaaa taattttcaa atgttgtaag ttgtggctaa agctttattg tattattctg
    13801 aatatagaaa gatagttact gtcttagata aaaactgaaa catacataat aatataaaat
    13861 ttgctatatt aataactcta attattttgg aaggagggaa aatatgataa gtgggagaat
    13921 gttactcatc atgaattttc taaaaggaaa aaataagaca agttacaagg atattagcaa
    13981 acaacttagt atagaagaaa gaaaagttag atatgatata aataatttaa atattgtttt
    14041 gcgttcatta aacaagtcag taatacaaaa gatgaacaaa ggtgtgttaa ttattcctga
    14101 tgacttccaa gtagtatgta taaataataa cgaatatgta ttttccttaa ctgagcgagt
    14161 atctattatg aaaattatat caatgtttaa aatagataat ctaaacttag aaaaattaag
    14221 taaagaattt caggtttcta gaagttcaat taaaaatgac cttaatgttt tgtcaagtga
    14281 attagaaaga aaccaaataa ctattactta tgataaatct tttaaaataa gtggagaaga
    14341 agacattata tataagcaaa gattgaacat attaaaatcg tattcttatc tttttggtaa
    14401 ggaaaaatct gatttaagtg tatttgagaa gtatttagtg aatgtaattg aaaaaatatt
    14461 caaagggatt tatttgattg atatatataa ttggtctatg aatttattat tgcaaatggg
    14521 atggacttta aatgatgaat cttttacatg gtatgtgtca aacattttcc tttttacatg
    14581 gtatatacat tcagatgaaa aacatccttt agaatctgct tcattaagtg agccgtgttt
    14641 tgaaaataag tttgaccaag aatttgaaac aattattcaa aaaaagttga ctaaggaaca
    14701 aatttttctg ttaaagagtt ttgtattttt tacaaataaa tatgcaagtt taaacgagga
    14761 tatggatttg attagtacag aaattatagt tcaagatcta atttacaata tgtcagaaat
    14821 attagagatt agctttaaag aagatatgat tctttataaa ggattactca atcatattgc
    14881 cccattgata gagagaatca aacagaatat acagatttat gaggaattac ttcatgtaat
    14941 accagataaa tatcaatatg tacttgaagc tactaaggtt tctattcaaa aaaacagagt
    15001 tttaagtcaa ataaagaatg aaaatgaaac aatacttttg gcaatacatt tcttaggaag
    15061 tatacataga aatgagagga ataactacaa aaatatattg ttagtttgtg gatttggata
    15121 tggtgtaatt gctatgttaa aggatagttt agtaaatgat taccagataa atatagtaga
    15181 gagtattcca tcatataagt tacaagatta taaagaatgg gaaagtattg acttcattat
    15241 aacaacatca aagatagtaa tagacttgcc taaaacaatt attgagataa atccattttt
    15301 acaagaagag gattatttga agtttgaaga aaaagggata aaaagaaaga acgtcttaac
    15361 taattatatg tcaataaaaa aaaggttgga ctttttagaa gaagatgccc aaaaaagagt
    15421 attaagtatt atatgtcaag aacttggata cagtgatgag agaatattat ttagacaact
    15481 taatttaagt gatttaatag gaattgatac cataaggata attgatggag atatgaaatg
    15541 ggaagatgct gtaaaaatta gttcaaacat acttgctaat tgtgattttg tatcagcaca
    15601 atatgctgag aatattatag atattttaaa aaatgtgggt ttttatgctg taaaagataa
    15661 cgaatttgct ttactacatg gcaatgatag ttcattagtc aaagtaagtt cgataagttt
    15721 attaatatgt aaagaagcag ttagttttgg agataaaaag gttaaaatca tattttgcct
    15781 agcaagcaga gataaaaaag agcaaattcc tgcaataatt aatctaacta aaatggttta
    15841 taagactaat tttattgcta tgttagaaag tgcaaagaca aaagaagaag cagaagtttt
    15901 aattcataaa tatgagaagg aggtcactgt atgaataaat tagttaatgt aaaatgttca
    15961 tttaaaatac atgttaattc aaaagaagaa gccattatat cgttagttga tgtagtgtca
    16021 aaagaaggat atttaataga taaaaatcaa tttttaaaag atgttttaaa gagagaagaa
    16081 acattgtcga cttatattgg acatggtata ggtcttcctc atagtcaaag tgtaggtgtt
    16141 aaaaactctt gtattacaat aggaaagctt gatactccta ttgaatggac agaagaagga
    16201 gaaaaagtgg atttaatatt tttgatatca gtaacaaaag acaatgaaaa taatcttcat
    16261 ttaaagattt tatcaaaatt agcacgttta ttgatgcatg aaagttttag aaatcaaatt
    16321 agatgttcag atgaacaaac tgtttataat cttataaaag aaaaaataga ggaggaagat
    16381 taaaatgaga attttaaagt ttttgcaaaa acatcttatg acagcaacat cttatatgat
    16441 tccatttgtg gttgcaggag gaatattatt tgctttatca gtaactttaa gtggacaagc
    16501 tgctgttcct gaaacaggct ggttggctaa actgaatcaa attggagctg ctggcttggc
    16561 tttattcatt cctatattag gtggatatat agcatttagt atggcagata aaccaggatt
    16621 agctccaggt atgataggtg catatttagc aaaagaagta aatgcaggtt ttataggtgg
    16681 tatcatagca ggatttatag caggatttgt tgttttacaa ttaaagaaaa ttaaattagc
    16741 tcctacaatg agaactttag gttctatatt tatttatcct ttagttggta cattgattac
    16801 aggtggtata attgtatttt taattggaga acctatagct tcttttatga catggatgac
    16861 aaactggtta aatggaatgt ctggtgtttc taaaattcct ttaggtggaa tcttaggagg
    16921 aatgatagcc tcagatatgg gtggccctat aaataaggtt gctgctactt ttgcccaaac
    16981 tcaagttgac acacttccat atttaatggg tggagttggc gttgcaattt gtatacctcc
    17041 aattggttta ggattagcta cattattgtt tccaaaaaaa tttagcaagg aagaaagaga
    17101 ttcaggtaaa gcttcactat taatgggatg tgttggaatt acagaaggag ctattccatt
    17161 tgcaacatca gatccagtta gagtaatacc ttgtattatg gctgggtcga ttgtaggaaa
    17221 tattatggct tttttattag gatgtttaaa tcatgcaccc tggggaggtc tgatagtgtt
    17281 acctgtagtt gacaataggt tggggtatat tgcatcagta ttaactggag cagtagttgt
    17341 tgcggtatta atgaaattag taaaaaaaga cgtgaaagaa gatgaagaaa ttgaagaaga
    17401 tttagatgaa tcaattgaat taatatttga agaattataa atataaatgg aaggaatttg
    17461 tataaaaaag taaatataaa actataaatt taaaggagat aagaatatga aaattgtagc
    17521 agtaacatct tgtcctagtg gagtagcaca tacttacatg tcggctgaat cacttgaatt
    17581 atcagccaaa aaatttggta tagaaataaa ggttgagact caaggaagct ctggaataga
    17641 taatgaattg tcacttaaag atattgatga agcaacttgt gtaatattga ctaatgatgt
    17701 tgagattaga aatatggaac gttttaaagg aaagaaggta gtaagaatga gtgtatctga
    17761 gataattaag aaatctgatg cattaattaa gaaaataaaa gatacttttc aataaaaatt
    17821 gatttatttt acatataact caaagatgtt tttaaagaaa tttttattaa aagcatcttt
    17881 gaaaatatct taaaaataga ataaaaggag atatagtcat gaaaaagtta ccaataaaga
    17941 ctgtcgtgca aagattacta aacttacaag aagaggggaa aagtgcaact cttttaggaa
    18001 ttggtcctat gtcaccaaac ttgttacaag caagttttga actagcaaaa gatgatgatt
    18061 ttcctttaat gtttatcgca agtagaaatc aagtagatgc agatgaacta ggtggcggtt
    18121 atgtaaatgg ttggaatcag gaaactttta caaaagatat taagaaggtt gccgacaaag
    18181 ttggatttga tggtttatat tatttatgta gagaccatgg tgggccatgg caaagagata
    18241 aagagagaaa tgaccatttg cctgttgatg aagcaatgga gttaggaaaa aaatcttatt
    18301 tagcggacat tgaagcaggt tttgacctat tgatgattga ccctacaaaa gacccatttg
    18361 aaattggaaa agtaattcca ctagatgtag tactagaaag aactgtagaa ttaattgagt
    18421 attgtgaaaa cgagagaaaa agacttaatc taccagacat aggttatgag gttggtacag
    18481 aggaaaccaa tggaggtctg acttcaactg agacgtatga aacatttata actagactaa
    18541 aggttgaact cgataataga ggtttgccaa tgccaacatt tatagttggt caaactggta
    18601 ctttaactag aaagacagaa caagtaggaa cttttaattt tagaaatgct tatgatttag
    18661 ctcaaatggc taagaaatat ggagttggtt taaaagagca taatggcgat tacttagatg
    18721 atgtaacttt attagaacat ataccatctc aaattattgc tacaaatgta gctcctcaat
    18781 atggtactga agaaactagg gcatatttaa aattagcaga agttgagtgt aaattagaaa
    18841 aagagggctt agttgagaaa acttctaata tcagacatgt tcttcttgtt aatgcaattg
    18901 aatgtggacg ttggagaaaa tgggtagtag gagaacaaaa aaatctaact actgaagaga
    18961 tatttaagga tgaagtttta tctaatgaaa tactagatat tgcaggacat tatacattta
    19021 ataatgatga tgttaaaaaa gaaatagaag tgctttatga taatttaagt aagaataata
    19081 ttgatggtca aagatttgtt gtagaccata ttaaacgtcc tttgcgtgat tatgcagaat
    19141 gctataactt aaaaggtgtt actacgagga ttttaaataa ataataggac ttatagtggt
    19201 gagtattgtt atactaggta tttatagtta taattatact taccactatt aaatttagga
    19261 ggactaatat tttgattaat ttaatttttg atgtagatga tactttatat aatcaattaa
    19321 cgccttttta cactgcatat aataaagtgt tttcttcaat aaaggatatt tctattgaag
    19381 atttatatat gagtagtaga aagtatagtg atgaggtatt tcatatgact gaaaatggag
    19441 aaatgccaat caaagaaatg catatttata ggataatgaa agcttttgag gagttgggaa
    19501 attctattac agaaaaagat gcacaaagtt ttcaagatga gtatatatat caacaatcac
    19561 aaattacact aataccagaa gttgagagga ttttaaattt ttctaaagaa agaaatatta
    19621 atctaggaat tataactaat ggcccatcaa accatcaaag aatgaagtta aagcagttaa
    19681 atattgaaaa ctgggttgat aaaagtaata tttttatttc tagtgaggtt ggattttcta
    19741 agccagatac taatattttt agagtagctg agaatgttat gaatttagat agagaaaata
    19801 catattatgt gggtgattct tatagaaatg atgtattagg tgctaaaaaa gctggttgga
    19861 agagtatatg gttgaaccat aggggacatg agttagaaga gttgttttat aaacctgatt
    19921 ttgtaatttt ggaacataag gatttgatat ctttatttat aaaaatatgt agttataaaa
    19981 atatgtagta aaaaaatgtt aataatagaa ttgtataaaa taattaataa taactatata
    20041 aaattatatg tttagattgt gagcaatcta ttttatgtta aaaatagatt atttagaaga
    20101 aggggaaatc ttaaaaaaat ttgaatatcc cctttttatt atttaattaa aatgaaatag
    20161 ttagaaatat attttattta gtttagtcaa taacattttg ggattcttta taactacatg
    20221 ttaactgttg aataggttta tcatatggtt taacccatcc tttattaata cttaattctg
    20281 taagtgctgt gtgaccttct accatttgag ttaatgaagc tgaatatata gctcttagtt
    20341 caggtgtact acttgtcaat gcagaattaa gatacatatt ggcagatgct ttagcagatg
    20401 aaagcatact taacattatt actttatcgt ctatataagt attgttctta attagatttt
    20461 ctatcatatt tctcatgtta tctatacctc ctcagaaatt agtatttcat tttcaactat
    20521 aaattgttgt atagctttaa ttcttccttc tgttgctaat atactagatt ctgattgtct
    20581 ttttagatct tcatctgata ttaacatatt tattgctctt tgcataagta atccgtcaga
    20641 ctccattttt ataattgctg ctaaagatat tttttcagtt tctgaaagta ttctcattta
    20701 atcatctcct atattgtaat ttatgcttta atagtatgtc tttcttttca aggaaataac
    20761 cattacactt ttttacagta ttttatattc tcgttatatc aaaatttagt cgacttaata
    20821 tattaatata attttgtagg atatttatca ttaaatatct ataaaataaa ttgtttgaat
    20881 attcataaag caaaatttat ttgtatgtac ttcttcaaga gtaaatatga gttttttcaa
    20941 aaaattaatt tttatgataa ttattaaggg ctatattttt cttaaagttg tgattatcat
    21001 tattaattta caataaaatt gccgaaaagc cctctccttt aggtgtaggg atgaaaggta
    21061 aggtcttaat ataaaaaagt tagaataatg cttgtaatct aataccgcta
//
LOCUS       DAMJZE010000053        20874 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJZE010000053 DAMJZE010000000
VERSION     DAMJZE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.053"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(39..899)
                     /locus_tag="PC541_001740"
     CDS             complement(39..899)
                     /locus_tag="PC541_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2839206.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEIEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1216..2544)
                     /locus_tag="PC541_001741"
     CDS             complement(1216..2544)
                     /locus_tag="PC541_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2839207.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2692..3021)
                     /locus_tag="PC541_001742"
     CDS             complement(2692..3021)
                     /locus_tag="PC541_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2839208.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3045..4334)
                     /locus_tag="PC541_001743"
     CDS             complement(3045..4334)
                     /locus_tag="PC541_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2839209.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4350..4676)
                     /locus_tag="PC541_001744"
     CDS             complement(4350..4676)
                     /locus_tag="PC541_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2839210.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4811..5440)
                     /locus_tag="PC541_001745"
     CDS             complement(4811..5440)
                     /locus_tag="PC541_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839211.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5655..8312)
                     /locus_tag="PC541_001746"
     CDS             complement(5655..8312)
                     /locus_tag="PC541_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839212.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8671..9615)
                     /locus_tag="PC541_001747"
     CDS             complement(8671..9615)
                     /locus_tag="PC541_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839213.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9608..10633)
                     /locus_tag="PC541_001748"
     CDS             complement(9608..10633)
                     /locus_tag="PC541_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839214.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10708..12276)
                     /locus_tag="PC541_001749"
     CDS             complement(10708..12276)
                     /locus_tag="PC541_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2839215.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12389..13300)
                     /locus_tag="PC541_001750"
     CDS             complement(12389..13300)
                     /locus_tag="PC541_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839216.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13284..14270)
                     /locus_tag="PC541_001751"
     CDS             complement(13284..14270)
                     /locus_tag="PC541_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839217.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14630..15031
                     /locus_tag="PC541_001752"
     CDS             14630..15031
                     /locus_tag="PC541_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2839218.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15034..15432
                     /locus_tag="PC541_001753"
     CDS             15034..15432
                     /locus_tag="PC541_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2839219.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15784..16008)
                     /locus_tag="PC541_001754"
     CDS             complement(15784..16008)
                     /locus_tag="PC541_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2839220.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16043..17410)
                     /locus_tag="PC541_001755"
     CDS             complement(16043..17410)
                     /locus_tag="PC541_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839221.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17494..19029)
                     /locus_tag="PC541_001756"
     CDS             complement(17494..19029)
                     /locus_tag="PC541_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839222.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19052..19486)
                     /locus_tag="PC541_001757"
     CDS             complement(19052..19486)
                     /locus_tag="PC541_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839223.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19746..20657)
                     /locus_tag="PC541_001758"
     CDS             complement(19746..20657)
                     /locus_tag="PC541_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2839224.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
BASE COUNT         6579 a         3715 c         2172 g         8408 t
ORIGIN      
        1 ttatataata agtattttta ggattctgtt acataatatt atttaaatat tcttctatat
       61 ttctcttctt cttcttcagt aaacacatta aatacaactt ttaaattttt ttcaggattt
      121 tcttttagcc atagtctaac tctacttaca gcaagattag ctgcctcttt ttttggataa
      181 ccaaatacac ctgtagaaat tccacaaaaa acaatatttt ttatttcatc aatttctttt
      241 attgtattta aacatgattt ataacaatga agcagttgct tttcttgctc tttacttggc
      301 tgaccaccag atacaatagg tccaacagta tgtactacaa attttgcagg taaacaataa
      361 cctctagtta tcttagcatc tccagtatat tctaaatgcc cttgtttttt tattattttg
      421 tcacaatctt ctctaagtct aggacctgcg catgaatgta tctcattatc cacgcataga
      481 tgtaatggtt gtaaacaccc taataacttg ttatttgcag catttactat tgcatctgct
      541 cttaaattcg ttatattacc tctccatatg gcaatccctt cctctatttc ttctatatca
      601 tttacatcta ctaactctct ttcttcacat tcaatcaaca ataaattatg taacatatta
      661 agtatctctg gagcaatttc ttttggtgga tatacattca aaagtcctct aagtatatct
      721 ctaggcttaa tttcatcatt tgataaattg tatattttag aaggaacttc ttttgaaaaa
      781 tatgcaatta aagtgtttat attttttctt ctttgttcgt cagttagagg cttgatagtt
      841 ttatcaaaat cctcaaataa atttacatca tcagcataat ctctccactt catagccatc
      901 tctccattct tgtattttag acttaaaaat aattatgtat ttatgtacat atttatttat
      961 ttctataaac acctacataa atcctgttaa aagtgtaaat tttttattat gcaacttctc
     1021 tggttatttc tattgattga tagtaaatta ttgtacctaa atttaatcca actaacaagt
     1081 aactaaacct actttcctaa ctgcaaaata cactatgttt gccacttgta tctcatttgt
     1141 aaataaataa agtccctaca aatacattaa ctaggctatg tactaaactg taggaacttt
     1201 aaaaatatat tattcttaat taagttttgg ccaataattt ttatttgctt ctattaaatc
     1261 atctagtaat tcttttgcaa ctgttgcact aggaactgtc ttagatagca taagtgcttg
     1321 ccacaattta ttataagact tctcaatcca agcatcaact actaattttt caactgctaa
     1381 ttgttgttct attaaacctt tttggaatgt tggtattttc cctattgata atggttcata
     1441 cccgtctttt ccaactatac atggaatttc aaccatagca tcatcttgta tattttctat
     1501 agctccatta ttttgaacta tcaataacat acgctcctgt gtattaaaag ctagagctgt
     1561 agctaaatcc actataaact ctgcatgtat tcctatctta agcccactgc ccttagaatg
     1621 tccttgcttt tctatcaatg cacatgaacc aaaaacttct ttttctcttc catcaataac
     1681 ttcattggca cgagtataat ttacatcaga atgatttaca acataatctg gaaatagata
     1741 gtactttaag taactacttg gtattgtagt taaatctgtt tttactattt ctttagtaaa
     1801 taaattagtt tcaaaccaac tatctttctt gtcatttttc attccttctt caccatttat
     1861 aaaaccatat tcttttatat gttctactaa ttttggtaaa agttcatttc cttttttatc
     1921 tttaattgaa gtataccaac caaagtggtt taatccaaag tatcttatat ccatatgttt
     1981 acgagatttg agtcctgcaa tccttgctat attatgttcc atacctattg gcatatcaca
     2041 aatatttatt accctagaat taggtctcaa ccttctaaca gcttccgcta ttattgatgc
     2101 tggattagaa taatttaaca tccaacaatt tggagaatac ttttccatat aatctatgtt
     2161 ttcaagaaca ccttctatag acctcattcc ataagctatt ccacctggtc cacaagtttc
     2221 ttgaccaact accccatgtt ttagaggtat cttttcatct agttctctca tttcaagttt
     2281 tccaactctt atatgagcta aacaaaaatc tacatctgta taagcctctt tagggcatgt
     2341 tgttgctaaa tactctatat tagggtcctt ttcctttatt agtatttcac aagctttagc
     2401 taccttattt tgtctttctt catcattatc atataatttg attgctctta atggtaacct
     2461 atctaagtta tctagcaaca ttaatatgat ttctggggta tatgtacttc ctcctccagc
     2521 tattactact gaaaattttt ccataataat atctcctttt ctatttaaaa atttactgta
     2581 tttataaata aatttataaa tactagtact ttaaagtaaa agggagttca tttattaaat
     2641 ctattgattt aataaactat agccaaactt caagcactat ttacaagaaa tttaattctt
     2701 attaattctt gggtccatca tctcaaatat atctacaagt tcagatacta aagaatgagc
     2761 aaataatgca tttgaataat ggtcttcaga gtgcacaact aaaaaatcag tcccactatc
     2821 ttcactattc atagcatttg atagtaattc taaatgatgc tttgatgctg tttgtatttt
     2881 ctctgaggct gaatttaaaa ggtgttttgc tttattgtag tcaccttttt ttatttcctt
     2941 aagagacatc attacatcac ttttagcatc tcctgcttct gatattatac taaatacttt
     3001 ttcttccatt tcaggtacca tatccttctc cttctaatga cttgttaaaa atcaaagcta
     3061 tcatcatcta atgttgtagc taattctgca tttttattta attcttcctt ctccattatc
     3121 ttaaagaatg gataatatat caaggtcata agtattatac ttgcaaatac taatacgatt
     3181 gccttccaat ctaatgtcat caaaaatgct gcaacccctg gaggtaaaaa gcctggtggt
     3241 tctgcaatag gtaatcctac taatcctact tttaatgcca taaaattaag tgctccaacc
     3301 aatacaggtc caaatacaaa tggtacaaac atatatgggt tcataactat tggtgttcca
     3361 aatattaccg gctcatttat tccaaatagt gatggtaaaa tcacaacttt tcccatacta
     3421 ttaaaactct tacacttact tcttgacata aggtacacta atcccaaagt tatccctgaa
     3481 ccagttactt gcaagaaacc atagtaaaaa cctattgtaa acacatattt tgggtctcct
     3541 cctgctgcta ttaacgttgc attttctgcc atatatgtag ctgctattgg catccatata
     3601 cttgaagtta ttgctgctcc atgaagccca aagaaccata ataattgagt tataaatagt
     3661 attatagtta ctgcaaaaac attatctaca ctgcttatag ctggtgctaa gaaattcatt
     3721 ataatagctg gtaatggttg acctgagaca tttttagtta ttgctgctat tgctgtaaat
     3781 aacattatac atactatcat tggagttaag tttctaaaac ttttatttac tacactaggt
     3841 acttcttttg gaaatctaat tacgagattt ttatcattca taaacttagt aatatcaact
     3901 actaaaagtg ctactaatat acttacaaat aaaccttgtg catctaaata acttgtatct
     3961 aatacaccat ctactacttg tccacataca acaagttgtg caacaactgc tgtcatacct
     4021 atttctacat gattaagatt atatttttta gctagtgcct gagctataaa aaatgctgaa
     4081 tatatagata ataaactcat agtaaattta ttagggataa gtaatgtatc catattttca
     4141 gataataatg tttttgcact tccttcaggt aacatattgt ttaatgccat aaataacata
     4201 cttaaagaac caacaattat tattggggta atgcttatca taccttcttt tatagcttga
     4261 agatgccttt gtttttcaat cttttgtgct attggaagaa gaaatcttct taaattattt
     4321 tgaagtgctt ccatttttat tccccccaat tatctatttt tatataattc gattgccgac
     4381 ttcataacag aaggagcatc catacgacca taatgcttag tatctattaa accaactgct
     4441 attttgtaat tcttacaagc ttcaactaca tcatcttcct taaacctaat ttgtggtgct
     4501 acaagtacta catcggtatc attagcttca atgtatgatt ttaaattttc aaatgatgtt
     4561 gcctctatat ttatagtttt agtttttaac ttttcatgct cactacaaaa acttttcatc
     4621 ttttccacaa gtacacctgt tgaagctcca tttgcacata ctaataaaat attcataatt
     4681 accctcctac aatatctaac ctcttttatt ttctaatttt ttatcagcca attcttactc
     4741 taattcctat cagtttttac tttaattctt attattttat tttaattctt attaatattt
     4801 actctaattc ttagtactgc caatgattaa acttgaaagc cgtatctata acttcttctt
     4861 gcttatattt ttctaatatt tttaagtatc tttctttata atcattatct acttcttttt
     4921 ctggtattac tatactagct tcatttaaaa agtggaatga atcccttccc atttctaatc
     4981 ctctatatgt atgcttgtca agtgccacgt ccataacttc tggaacttta cccattgcaa
     5041 atccttttat aattatattt tttaataaat cacttgatct atctttctca catgcacaaa
     5101 gatatcttat tgcatgtatg aaaaataaag gtctgtctcc atctgcatat gaatattcct
     5161 ttcttacttg atttaaatta tttatgatta ttacagctga tgggtccccc attcctatat
     5221 cttcaactga tattgtaagt atccttctcc atagcttatc ttctaattct ggtgatgata
     5281 tatatagttc ataagcaaat tcacaagctt cttccaccat atttcttctt atagcttttt
     5341 gaaatgacga tattatttca tctgcactaa atccgtttct tgtagtcatt cttgcccatg
     5401 ggtcatttgc ctttttatct cttaatattt tatcttgcat attatttact cctttttatt
     5461 taaattttaa ttatttatat tactcattta ataatataag caagtattat gccagttttt
     5521 ttagacactt taaaacactg ttatttcaat catttttatc atcaaaactt agatactttg
     5581 tctaagtata tttatatgtg tctaatatta atattatttt agactaaaaa aaattaccaa
     5641 ataagttttt atactcattg gtaatctttt tcgattattt ctgcatattc taatattaat
     5701 aatatttgat agcactcttt ttcattaatt tctatattat attttttatc taattgagat
     5761 actatatctt ttattttgct taggctcttg tgataatcaa gtgttttttc tttatcaagc
     5821 ttttcaattt ctctagtatt tccaatcaat ccatctacca tgaaagctaa atgcatcata
     5881 agtccaatta ctttatcgta tttcttttct atattaaata ctaaaaacaa ttcatcagac
     5941 acatatttta tatctttaaa tatcctatta taattagagc tttttaagga tgtctttaaa
     6001 ccttcaacta catcatcata acctatactt atctttataa tattttttaa tttattgata
     6061 ttttcatcct tatctatatc ttttttgcta atatagtcaa ttcctctaat attaattctt
     6121 tgttcattga ttactgcaac aatattacta ctttctttaa gtttcgtaat atttaaactt
     6181 aaatcttttt ctgttttata tgggacagat ataatatcta ttccatctaa gtacctactc
     6241 atcttttctt ctaatatttt tcttgtttta ttatctacac cttctgaaac atgtgatgct
     6301 ataataattg ttttagacag attttcttta ttttgaattt cattagttcc aaaatagtac
     6361 tttctcgtgt tagttataga gttgtatacg tcatttaaag gtaatcccat attggccttc
     6421 tgaactgctt ctaacacagt taatgttgtc accataggaa ttactcttac tccacacttt
     6481 ttttctttct ttataatttc atcaaaagag tttaatgaac ccatatctgt aaatagtaat
     6541 acatctttat tgtctatatt attaattaaa tttttaaaat ctacaataac ttcatcatag
     6601 cttttatcta acttcatatc gaaagaaata gcactgcact ttccaataat atcaccaact
     6661 gcttttacca tactacttgc tgtagtttct ccatgcatag caactataat attaatgtta
     6721 ttcactattt tattttgttc atcttgttta gcttgtacta tgaataatgt aatatatgct
     6781 acagtttcta tagataaaaa taaatcaaat tcattttcaa ttattccaac tatttttagt
     6841 gctgttttaa attcacttct gttatttctt ctaatttgat tcaaatctat gttgtttgta
     6901 gagctacttt tatccaatac gctttttata taaattgcca ttgacttaaa tattgattca
     6961 cttatgtcta tttcttcatc ttctttaata atttgtctta ttgattcaca catgctttta
     7021 aacttaacat tattaaatag agaagcatct gctatattca tttctacaga tttattaaga
     7081 ttagtattta ctgcattaat aatatttata tcattgtagt taataacttc ttcttctcct
     7141 aatgacgtaa atacaaaata ttctattcca tgtgttataa cttcatctgc tttaatagac
     7201 ttctctctat aatcatcact cacttcattt atagatttat caaaatattt tttctctata
     7261 taaactggtt ctttattatc tcttttatat cttaggtatc catttgcaac agctaatttt
     7321 atatcatttt gaagttgacc tacattattt ttacaatcat aaagtaatag tgatttaatt
     7381 atttctttat gtacttttat tgaaatagaa atactttttg attcttcctt gaaaaaattt
     7441 ttgactaatt ccattctttc aatcattgtc ctatctctca gtgatggtat ttctattttc
     7501 attggtattc ttctattgaa tgtatctaat agtgttgatg tcggattttc tgtagtagca
     7561 catattatta ataccttaga ctctctttct ttcgttgcat ctcctagtct tttaaatgtt
     7621 cccttatcta taaaagtaaa caacatttct tgaccttctg ctggtagtct atgaatttca
     7681 tctaaaaata gtatccctcc atttgcaatt tccaaaagac cttttctgga agattctgca
     7741 cctgtataag ctccttgttc taccccaaaa atatgtccca taagtaattg tgcattatta
     7801 gcataatcag cacaattaaa tgttacaaaa ggagctttat tttttctaat tccactatct
     7861 atagaataat cgtacatata tctagcaatc atactctttc ctactcctgt ttctccatag
     7921 ataatagtat gaagtccatt tgtaggatag agcattgcga ccttgcattt ttctattata
     7981 ggtcttaaac tatgttcttt acctattagt gtttcaaaac tatctttttt tattttgttt
     8041 tctacttttg ttcctttttt tatgccaagc aactcacttt gtgtatcaag caattcattc
     8101 ttgttaaggt taagatttgc agggatataa tacacaggtc ttccttttat cttcgtaacc
     8161 ataccctcat tgaataattg gtttaaatat gtacttatat tgtttcttgc aattcctgtc
     8221 ttttctgata attggttagc tgtaatccct ttttcatttt ccctatatga tgattctaaa
     8281 actttcatta ctttatcttt tttatatatc attacaagct cccctacatt atagtatttt
     8341 attaactaaa acccctatac acactttatt ttttaataaa ctcttattct ttaactaagg
     8401 ttataattta tcaaactttc cttactttaa attatagaaa attattgtag aaaaaataag
     8461 attattgctc attttttcta cattactatt tttaatttta tagtactttc ttaacaacat
     8521 actttacttg tttttaattt tattatattt gaatttatta tatttgaaaa gtagaaataa
     8581 aaaaccctat tttggagtag ttttaaaatt tctccaaaaa tagggtttta gtttgtattt
     8641 caaataaata cctgttcaag tatttttata ctaaacaact tttaaatttt gttgatattt
     8701 atttatttct gatttagaag aatatacaaa atgtccatct gaaacatcaa tccaacttaa
     8761 tgacttttca tttgaacaat catatccatt agaattatac tctattctat ttcttgactt
     8821 agcatatcta gggtcaggaa caggaattgc agaaagtagt gattttgtat atggatgaat
     8881 aggattatta tacaattcat ttgaatctgc caattctact aattttccat tatacataac
     8941 tcctatacgg tcactaatat gtctcaccat agataaatca tgagctataa ataaacaagt
     9001 taaattcttt tcttgttgca attttttaaa taaatttact atttgtgctt gtatagacac
     9061 atccaaagaa gctataggct catcagctat tataaattct gggtctactg aaagagctcg
     9121 tgctatgcca atacgctgtc tttgtccacc tgaaaaatca gatggatact tatttgcata
     9181 acttctatca agtcctacta gctttagcat ctcatatact ttattcattc tatctgtttt
     9241 atttttacag ataccttgaa tttttagtgg ctctgaaatt atatctgcaa ttgtcatacg
     9301 tggatttaat gatgaagtag aatcttgaaa aataatctgc atacttttgt ttacatcttt
     9361 ttttataaat ttgtacgttt ttttatctga tatgcaattt cctttgtaaa ttacttctcc
     9421 accagtagat tcatgtagtc ttattatagt tttaccagtt gtagattttc ctgaacccga
     9481 ctctcctacc aatccaaata tctcaccctt atatatatca aaacttatat catcaactgc
     9541 ttttacagta gtacttttgt ccaaatgaaa atattgcttt aagtttttta tttccagtat
     9601 tttttctcta ttcattagaa atcacccttc cacaatttac tctaactggt acatctactt
     9661 caggtgcatc tgggtgcaat aaccaagttg ctgcactatg tgtatcatta attttaaaca
     9721 ttggtggctc tttttcataa tctatcttaa gtgcattttt attccttatt gcaaaagcat
     9781 ctccttttgg tgggttcaat aaatttggag gcattccagg tatattatat aaatagtcat
     9841 cctgcgaatc taaagtaggt aatgaaccca acaaccccca agtatatgga tgtctagggt
     9901 cataaaatat atcctctaca gttcctattt ctacaatctt tcctgcatac ataactgcta
     9961 ttctatcagc tatagttgca actactccta aatcatgagt tataaatatt attgacaaac
    10021 ctattttatg ttgtaaatcc tttattaaat caattatttg agcttgtata gttacatcta
    10081 aagcagttgt aggttcatca gcaatcaata catctggatt acaagcaagt gctatagcta
    10141 ttactatacg ttgtctcatt ccacctgaaa aatgatgtgg aaattgttta aatcttttct
    10201 caggattatc aattccaacc aattcaataa gctctatggc tctctttttt gcctcacttt
    10261 tacttatacc ttgatgaatg attacagctt ctgcaatttg ctttcctatt gatatagttg
    10321 gattgagaga tgtcataggg tcttgaaata tcattgatat attctttcct cttatatctt
    10381 ccatctcttt ttcagacttc ttaaccaaat cacttgattt aagtaaaacc tctccatttt
    10441 taatatatcc attgtatggg agtatcctca tcaaactctt acacagtaca gatttcccac
    10501 aaccagattc tccaactata gccaatgtct cacctttttt tagattaaaa gatacatttc
    10561 ttacagcctg aacttctcct tcttctactt taaaacttac agatagatta ttaacctcca
    10621 ataaatgttc caaaaaccat caacccctat gctattcaaa ccttagggat atatttttat
    10681 ccctaaggtt tctaattaat ttcagtatta ttcaattgtc cagtcagcta tgttccagaa
    10741 tatacctaca ccatgatgac ctaaaactgt atcaggtgtt aatcctttta tgtttggttt
    10801 tccaacatat attgcatcta tataagcaat aaatgtataa ggcatatcct tagtcatttc
    10861 tacttggaat tgtttatata actttaattt ttcatcttta tcttctgttt ctcttgcttt
    10921 ttgaagtatt ttatctatag taggattaga gtaagcactg taatttgcac ctttatctgt
    10981 tccaaatact ttatatgtat ggtcatctgg gtcaaatgga cttccccaac ctattaaatg
    11041 agcatcttga ttagcccaat ctgtttcagt tacaacaaca gctttagcat caacaccaat
    11101 ttcttttaac tgttgtgcac atattttagc catatcaact ctaacttggt cactctcacc
    11161 tgctgttatc tcgaaagcta acttagtacc ttctttttca tagattccat cagaacctaa
    11221 tttccaacct agcttttcaa tttcttgttt agctttttct ggattatatt caaacttttc
    11281 tatatcagga ttattatatg gtcccatttg taaaggtgaa tatgctggta caccatgtcc
    11341 taataataca ctgtcaacta ttgcttttct atctattgca tagcttaaag catttggtaa
    11401 tcctttagct tttttatctt taaagaattt agaattaaaa ttgtataata tacctctata
    11461 gtcggctgtt ttcattatat ttactttaaa attcttttcg tctttttcaa agttagacat
    11521 atctttagga gttatttgag ctaggtctaa ttctcctgat tttaattgca ttgccttagc
    11581 cttatcatct ggaacaatct taaagactac tttatctaat cctggttctt ttacaaaata
    11641 atcactattc ttaacaagtg ttatactttg acctttgtcc catttttcaa gtttaaatgg
    11701 tccagtacct attggttttt gattaaattc atcagtagct atatccttac cttccaaagc
    11761 atgttttggt aatactccta ctgttaaata atcaagcatt gctgtatttg gagcttttaa
    11821 agtaattttt atagtattat cattaaccac atcaattttt gttatatctt cataatttga
    11881 tgctatctca gaagcattat ctggattcat tatagtttct attgtaaact taacatcatt
    11941 tgctgtaaat ttttctccat catgccactt aacatcatct cttaaattaa aagtatatgt
    12001 atttgttgcc tcatcaaatt tccagtcctt tgctagacaa ggaaccacct tattattttc
    12061 atcatgagct gttaatccat taaatattaa cgaatttatt tctccatgtt catatagtgc
    12121 aggatttata cttgtatagt cattacttcc ataaacaagc acctttccat ctttaggcgt
    12181 atctgccttc ttatctttgt ctcctccact agaacaacct gccattagac taagtatcat
    12241 tactaaactt aaaactttta acttctttaa tttcatgatg ctcccccttt tgctttttat
    12301 aaaatatttt tcaaagcttt gaccccattt atttaaatag atatttcaaa tacctcagta
    12361 tttgcttatt taatactcaa atgctgattt ataaattact agatttttta ttattattct
    12421 ttcttatata attacctata tttgttatac acaccaatgt ggtaaccaaa aatatacccg
    12481 gaatcagtat aatccaccat ctatttgaaa gtaatgcttc ctctgacaaa gataacatac
    12541 ttccccaaga aacaatttct attggtaaac caattcctaa aaagctaagt gtcgattcag
    12601 taccaatagc tgcgcctatg tttgttacaa ccatgaacat tattgatgcc acaaaatttg
    12661 gaagtaaatg ttgctttaat acatagaaga atcctcctcc catgctctta gctgctagta
    12721 tgtattcgct attttttatt tgtctaactt ctgttctcac tatcttagat atattcatcc
    12781 aacttgttat ccctattact atggacatag atactggatt tgaatttcca agtatagctt
    12841 gtacaaatat tataattaat attgaaggta tactaagtat gatttctgta aacctcatca
    12901 ttgcatcatc tacaagttca gatgctgaac cacttatact accataaaca attcctataa
    12961 cagttgatat tacagtggaa aatagtccta taaataaaga tatttttcct ccataccata
    13021 tcatagaata tatatctcta cccattgagt ctgttccaaa taaaaaagtt ttatttggag
    13081 ctaaattaga acttgctaaa tccatgtatg ttggttcatg tgtcattata aaagatgaga
    13141 atacacttcc aatgactatg attgataaaa taataattga tatatatggt aattctttaa
    13201 tttttttaga taaggttttc tttgtttcta tatgttcatc ttctgctact aaaatataat
    13261 tttctcctac aatttcaaaa tcactatatt gtggcttcat tagtatcacc tctatcatat
    13321 ttcattctag ggtctatttt attgtttata atttgagcta acatattccc aaatactact
    13381 aaagcccctg ttattaagca taaaactaat aacatattat aatcatgata ctttgcactt
    13441 tcaaaacaaa gtgttcctaa cccaggataa gaaaaaactt tttcaactac ataagttcct
    13501 cctagtatat gaggtatcga aatggccatt atactaatat atgatggcat tatatttctc
    13561 aggcaatgct taaaaacaat agttctatta tttaaacctt ttgctttaca taaaagcaca
    13621 taatcttctc ttatttcctc tagtaattta tttcttatca tatatgtgta ataccataga
    13681 tggcttaata taagtactgt aagaggtaag attagatgtg atatcctact taatatactg
    13741 gactcttctc ccattgaata agcaccacta cttggaagta tacttaagtt tatactaaat
    13801 ataagtatga gtactaaggc aacccaaaat gatggaatac agttggttat tgttcctaat
    13861 ttgcatatga ttctatcaat gagcttatct tcatgtaatg tacaaaatat tcctaacaat
    13921 aaggctagca caaaggtaag tatatatcct gaaccaccta atattatagt gtttatatag
    13981 acatcattta taacagaagt aacattttgt ttatatttaa atgaaattcc aaattcccct
    14041 tttgatgcat ttgaaatcca ttttatatat tgactgtata ttggttcatc caaaccaagt
    14101 tttttcatag cattttcttt ttcttgtgta ctcattcttt ctactccatc cccatagtaa
    14161 gagattaatg ggtctcctgg agcaagtctt gccatgtaaa aaacaataaa agataataaa
    14221 aacattacta aaataaactg caacaacttt ttcagtatat cctttaacaa accccaaccc
    14281 ccctttgttg caataaaaaa aaccacataa aagcaaacgt aaagtaacgt taaaaccttc
    14341 gtggttccat tatcattaaa caaaattaaa ttttctatat ttatctatga gttaatttta
    14401 gcatcattta ttaactgtgt aaacaaactt tttaaaaatt gttaaaattt catcaaatta
    14461 ttttatttat acctaaaata aaatttttat attctattct tcattatttt ctataaaatt
    14521 ataaaatttt aggttgacta tataagaaaa gtatattata ttagtttgta tgcaaaccat
    14581 ttaagaggtg attttattga aaagtaatta tgactgtttt agaatagcca tgctcttaaa
    14641 agaactttat tctaaaacta tgtacacagt agaagaaaat tttaaagaaa atggattaac
    14701 acaccaacag attatagtta taaaactgat agctcataat caagaactta caatatcaca
    14761 actatgtgat gaaatgtctt tagctaaagg tacggtttca ggcataatca gcagattaga
    14821 acagattgga tatatagaaa aattcaagaa atctaatgat aaaagaaata catacgttaa
    14881 gtttacaaca acaggatttg aatttgccac aaattttaaa attaaaatgc aagaaagttt
    14941 tgatgatatt tttaaaaatt gtgatgaaaa cgaattgagt gacttagtaa aaaatcttag
    15001 aaatatattg gcaaaagtaa aggagagata attatgaatt ataaattaat acttgcaata
    15061 gtatttataa catcagcttt aattttctac actataggtg tatttggaga aagaaaggca
    15121 aaaattttaa aaaagaaaca tgtaattata ttttggcttg gttttatatt tgatacttta
    15181 ggaacattta caatgagtaa catagccaac agccatactt ttgaagtaaa atctgcttta
    15241 tcacaaaacc ttcatagtat tacaggtctt cttgcaattg tgcttatgct ttttcatgca
    15301 agttgggcta cttttgtcct atacaaagat gatgaggaaa agaaaaaatt ctttcataag
    15361 tttagtatag ttgtatggac tatatggtta gttccatatt ttataggtat gtttattggt
    15421 atggctggat agtaaaataa aaagtgtgta agaaatactg taggtaaatt tgtataagac
    15481 aaatttatct acatccttct ttcacacttt tacatattta tttataatac tccattattt
    15541 ctttcatgtt tcttaatacc tatatagaat atttaattgt ttctatagta ttttattatt
    15601 ttctcacact ttttcatata tatattgttt tactatttct tcatatgtat atatgaaata
    15661 ttccattatt tctataaatg ttttattatc tttctcatac tttttcatat atattgcttc
    15721 actatttttt ttacttcttt attaaatact tatgtataac atacatttaa taaacgctaa
    15781 ctattaaaca cctaatactt gagttactcc tggaacttca ctcaccaaaa cattttcaat
    15841 aatagctttg attgtcatag tcactcctgg acacccacta catgcccctt gtagtctaac
    15901 taaaaccaca ccattttcat ttacatctat aagctctaca tctccaccat ctctttgtaa
    15961 tactggtttt atcttttcct caagcacttt ttcaactttt tctctcatag ctattccccc
    16021 cagaaatatt ttattagcaa aattatacca atggtttaaa tttttgtcca tctaatgatt
    16081 tatcaaattc aatttttgct tcatcaataa ttttagtatc cataaataca tcataagcac
    16141 tacaagccag tactttagct gaatttatca tagcagaaaa tcctatattt gaacctgctg
    16201 atgaacatga ctgccatgta tgacttgcaa ttccaacagg ccaagctgcc attgcaaatt
    16261 gagccactgg tattacataa cttacatctc caacatcagt agaaccagct agtcctttat
    16321 ctttgtcacc gtcaactatt tccttgtgta tatagctttc tacaatagat ttatcatttt
    16381 gtaatgatgt tgctacacct aatctttctt ctctagtaag agtatcacac aacttttttg
    16441 caaattcttc ttcttcagga gtatttttag ggcaaccaac aaaaaccata ttgtcataaa
    16501 ctaaatcagt caaacattgg tttggtatat agtcatatat tccatctgta accttatact
    16561 ccatttcagt ttctgtcatc attgctgctc cttgagctac ttttattaat ctatcaagaa
    16621 cgtgttctgc atcctttgct tttttacctc taataaagta ccaactttca gcaaatccag
    16681 gaactacatt aggtcttcca ccaccattag ttataacata gtgcattctt atagagtcaa
    16741 taacatgttc tctcaaataa ttagctccaa cattcataat ttcaacagca tcaagtgcgc
    16801 ttctaccatt atgaggagct tgagctgcat gggctgttat ccctttaaat ttaaatttta
    16861 cagataagtt tgccagactt ccacctctcc aaggtgcatt tatatcaaaa ggatgccatg
    16921 taaatgcaca atctacatca tcaaaacagc catttataca cataactgtc tttcctccac
    16981 cttcttcttc agcaggacaa ccaaaatatt ttattgttcc attaaattta ttttcttcta
    17041 ttaatttttt tatagaaaca actgctccta cacctgctac acccaacagg ttgtgcccac
    17101 atccatgtcc agcttttcct tctaccaatg gctttctttc tgcacttaca cattgtgata
    17161 gctctggtaa agcatcatat tctccaagta tgccaatcac aggtttaccc tctccaaaac
    17221 ttgctataaa tccagtaggt aaattttcta cttcttcaat tttaaatcct tttgacttta
    17281 ggtattcttt ttgaatgcta cttgcatact tttcttcaaa ggctaattct ggattttccc
    17341 atattttctt agataaatca attaattctt ctcttatttc ttcaacatta tttatgatat
    17401 actctttcat aattttcctc caaatatatg tatttttgat atgctttaca gattatatta
    17461 tatactaaca ttctatttat tcacaatttt ttgttaatta attttattca tatgctcttc
    17521 tatggagtat atcttatctt cactttgcct cttcataaat ttaatgtact ctatcttcca
    17581 taaagtagtt tcagttttct catttaacat aaattctaat atattaattt ctctcattct
    17641 aatatcttca taaaatacta cactacccaa tattgccaat tctaattcac ttaatgatgc
    17701 aaacaacatt aaatactctt ctccaaataa acattcagat tcaagaatat cgataaagtc
    17761 ttttttagca ttgatatttt cctttattat tcttattttt tcaattatag atgtactatc
    17821 taatatctca ttgaatatct ctctaaacga ttcatcatcc aatttacttt catcatcaac
    17881 aatataatct tctaagtgtt cctttttatt tttactcaca gttaccatac ttttaaaagt
    17941 gttacttttt attgagctta gcacagattc aataaatatc ttttcatatt ttttaatata
    18001 tcttattaca tcttcatctt ctatgcttaa tctttttatt aatacttcaa ttgcttccat
    18061 aagtagtaaa gttatttttt tatttattct aatttctctg ttaatttctt caaaatcatt
    18121 ataatccaaa ttactatcta cttcagtgtt aaaactatca tctacttcaa tcaattcaat
    18181 tatattactt tcttcagagt ctataacttt atcttcagta atattattgt acatattttt
    18241 aaattgtttt tctaaaaatt catactcata ttttgaaatt tctaaattag tcacttcatt
    18301 tcctaaaata gtagaaaaaa tacaattatt cataataagt tcaaataaat ttacaagtat
    18361 aacatcacaa tctattttat aagtcatccc atagtcataa aataattttt ctctatcttt
    18421 tttcttaaat aggttacaaa atctattttc tatatctata ctctcaatat aattcttcac
    18481 ataataaata cccttaacat ccatatcatc tagtgccaat ggataatcta tacttactgg
    18541 aacgtcctga gcttcaaatt ttacaatata acttttaaag aaacttgata actccatcaa
    18601 ggtatcatta tatgctattg aattagtatc caattttcta ctcatcatta gtttataaaa
    18661 atcttttagc ctaactaact cttcctctaa tatttcaatg cctccaaggt acactttttt
    18721 aacattctta gcagtcaact ccacaactct tttttctata tctccaagct tatttatata
    18781 tgcatcaata gcataccata ttgaaatcaa cagtttttcg gcaacttctg tttttactga
    18841 tgtgctttga ccatttgtat attttttaat caagtccata agtattagag atacttcttg
    18901 ttgtatatta tatacttctc tttcatctat cactttacaa tttagacatt ccttaagtag
    18961 agatatggaa tattggttct ttattaaatt attttcgttt atattaatat tacttaaatt
    19021 atttaacata caaaatctct cctatcatat attaaacttc tccaaattta aatttctttg
    19081 caaattcgtc tgcttctcct cttacattgt ccatattgcc tgaaaatttt tcataaaatt
    19141 cacccagtat ttctataatc tcatcatcac atatcttgtc atcagtttca ttttttaaat
    19201 aataaaaaat ttcttgcata tcattaatag cctctaaata attgtccaca tttgtatatt
    19261 gtgatgtata tatattttct attaactgtt ttgtaacttt tatatctaat tctattcttc
    19321 catacccctt taaagtatta tctctcgaat ttattatatc ttttacatca gattcagaaa
    19381 gagctagacc ataatctcca gattctatat tagtttcaag cagttcattt ctttctattt
    19441 cctttaaatt ctgatttata gaactcaaaa taataggtga tatcattttt aaattctcct
    19501 tttttatttt ttccattgac tttttttgtt ttttatgcta tacttaaatt aatgtatatt
    19561 attttaccaa ccacttataa tagtagtgtt aaaagtggtt tttgtcaact caaaattact
    19621 atctcaatta aacttttcaa ttttctcaac aaaaaaatac catcatttag atatataatt
    19681 ttctataaca acttttaatt cagtagttat ttactaaaat ctaaacaatg atatatttat
    19741 taattttatt tatctttttt ggctctactt ctacttttct ttacatttga gcaatttcta
    19801 caagaaggtg aacagtattg agatttagaa tttgtaggaa tataaatttt accacagtac
    19861 ttgcatttat ttaaagaaga aggttcactt gtaattgcaa aggcatagat agtctcaata
    19921 gctgttttca aagaatcaaa ctcccatgca ataattggtt tatctaattg gcttatagaa
    19981 aatcctatct tttgaggatt aaaccctcca gacataatag taacactttc agttaataga
    20041 ttagatgtat ttttatatac acaaatttga ttaaaatgct tacacatatc tttagcaaaa
    20101 cttacaatcc aatctagttg ttcagtgtag aactttgaaa atactaaatc taaaatcaat
    20161 ggtctctttc caaaaaactt tggagattct tctctctttg atacatctat accccttcta
    20221 tactttttaa aatacacatc ttcatcactt gcaaatggta taaacatgtt tacatattca
    20281 tctgtactca atatattctc ttttgtaata taattctttt caatcatcag tactctttct
    20341 tctccaacta tatctctatt atatacactt gaatatatta aacccaataa tccatacttt
    20401 tttacaaatt caagtacttc tttctttagt ttcatttctt ttgtctcttt tgatatatct
    20461 tgtaatgcct cttgtcctat gcgaattata tctactatta tatcatctgc tacatcaaat
    20521 gggttataca tggtaaacac agcatcttct gttggaacta tatataattc atcttcttca
    20581 gtaattttat attcataatc actataccta atccaatcac ttgcaaaatt ccctattaat
    20641 tctatttttt tattcataat aatcattcac acctttcaat gtaatatata acttcatatt
    20701 actattacac ttttagttta ttccatattt tctacactgt caactattct ttttatattt
    20761 ttagcatatt aaaatttata atagccttta taatggattt ttacagctaa atttcttgtt
    20821 tattgatgac taattcataa ttaaatttat aaaaactatt tacatataaa atta
//
LOCUS       DAMJZE010000054        20768 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJZE010000054 DAMJZE010000000
VERSION     DAMJZE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.054"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(25..867)
                     /locus_tag="PC541_001759"
     CDS             complement(25..867)
                     /locus_tag="PC541_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2839225.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1128..3170)
                     /locus_tag="PC541_001760"
     CDS             complement(1128..3170)
                     /locus_tag="PC541_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2839226.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3322..4293)
                     /locus_tag="PC541_001761"
     CDS             complement(3322..4293)
                     /locus_tag="PC541_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2839227.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4294..5271)
                     /locus_tag="PC541_001762"
     CDS             complement(4294..5271)
                     /locus_tag="PC541_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839228.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5490..7049
                     /locus_tag="PC541_001763"
     CDS             5490..7049
                     /locus_tag="PC541_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2839229.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7155..8114
                     /locus_tag="PC541_001764"
     CDS             7155..8114
                     /locus_tag="PC541_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839230.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8117..8929
                     /locus_tag="PC541_001765"
     CDS             8117..8929
                     /locus_tag="PC541_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839231.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9145..10047)
                     /locus_tag="PC541_001766"
     CDS             complement(9145..10047)
                     /locus_tag="PC541_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2839232.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10402..11580
                     /locus_tag="PC541_001767"
     CDS             10402..11580
                     /locus_tag="PC541_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2839233.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            11589..12245
                     /locus_tag="PC541_001768"
     CDS             11589..12245
                     /locus_tag="PC541_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2839234.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12245..12910
                     /locus_tag="PC541_001769"
     CDS             12245..12910
                     /locus_tag="PC541_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2839235.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            13015..13791
                     /locus_tag="PC541_001770"
     CDS             13015..13791
                     /locus_tag="PC541_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2839236.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13853..15187
                     /locus_tag="PC541_001771"
     CDS             13853..15187
                     /locus_tag="PC541_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2839237.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15683..16615
                     /locus_tag="PC541_001772"
     CDS             15683..16615
                     /locus_tag="PC541_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2839238.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(17007..20546)
                     /gene="nifJ"
                     /locus_tag="PC541_001773"
     CDS             complement(17007..20546)
                     /gene="nifJ"
                     /locus_tag="PC541_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2839239.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7175 a         3264 c         2919 g         7410 t
ORIGIN      
        1 cattctgtat taaactactc tttattataa attcagtaca gatataactc ttttgatatg
       61 taatgttaaa taaagtagtt catcttgact tacatagtaa tcataattct tttctacatg
      121 agtttttatt ttatatgcac aatcataatc cttttcatac tttactttaa ttatatctat
      181 aatttcatta tttgtatcat tgattttttc tttttttact aatcgttttg caaagaattt
      241 taaatgagtc agaagcctat cataatttat atcatcttct ttaaattcaa ctctataata
      301 atatcttata atatttagta tgtcttttac aattttagta attaacagtg attcctttga
      361 acttccctta tagtttgaat ttactatatg cattgctata aaaccagctt catctactgg
      421 aaatttcaca tcaaattctt tatttatata atctacagcc catttcccaa tctcatattc
      481 ttttttatgt attcttttta tctcatgtag taaatcattc tttatagtta tgttttcatg
      541 atatctttta attgcaaatg caatatggtc agtaagtgct acataaatat attcataaag
      601 tttagtattc aaattctcct ctgcataagc aattatttca gtactaacct caaaaaattt
      661 ctcatcaacc tggcttgcta gtttactaat tttatctaaa aagctttcat cttgtattac
      721 aaaagttctt tcaattttac ttttatctat ttcttgacct ttcttttttt gaaacccaat
      781 tccacatcct gttaatatta attctttttt tatattttca tctaaaacta atacaacatt
      841 attattataa atttgttgaa taatcataat caatatctct cccaaaatac aatttccctt
      901 attcataaaa aaatacctaa attaagatac aattcacctg tataccttaa cctaggtatt
      961 gcctgcttac cagtaacaat ccagcttgtt aacgcttata tattgtattc aatttatggt
     1021 tttattctat tataaataaa tatatatgtc aagtaaatta ttgaaaacta tttcctattt
     1081 tttagtatta attttaacat caaaaatcaa attttattcc attatattta tgattctatc
     1141 atcatcattt catcatagag aatattttcc aataactctt tagccacttc tcttgatatt
     1201 tgcttagttt caactaattg ttgaacactg tctctttgac tttgtatcgc cattactctg
     1261 tagtattcta ctcttgcctc aaacttatca tcattttgaa tctttctagt aatccaattc
     1321 aaataacgat tatagaatct gattgctatt aaggcctcat gtctgttatc ttcatcagta
     1381 tttttattta taaatgatat agcagtttta gtagaaagtg catatgcatg gtgaaactct
     1441 ttgattattt ctttattctc agatactatt tttgtaattt tttcttttac ttctttcttt
     1501 acttttctat ttataatcca catgcttatt tttaatttta caattttaaa tatagatttt
     1561 tgaacagatt ttcttgattt tgtaatataa aatctataca atttaaaggc tctatcagaa
     1621 attttaccat cttctaatag tttcaaaaca gctaataatc cctgttggtc tgcttctgca
     1681 atcatcatac ttataagttt attatctgga gtatttagta ttcctttttc tgagtctacc
     1741 aactgttcat ttaaatcatg tataactggt tgtacttctt gtgctttttc attactaagt
     1801 tttaaaatgg cttctttcaa aattagttta tatgcctctt tatcagataa taaaggttct
     1861 gactcttgta cgttattttt taataatctt ggcagtaaaa ctgttgccaa tactaaactt
     1921 ataagaataa cttctgatga tataaataaa acagtatctc tcattggaaa tacatcttta
     1981 tcagccaaat aaaaaggtat agataaggca gttgctagtg taaatgtacc atgaacacca
     2041 caagtagcaa ctattagtga gtatttacat ttagaaatac tctcttttac aacttcttca
     2101 tccttaaaac ctaaaaaacc tacaaaaaaa ttatttaaag gattcttttt aggttttata
     2161 aaagaattat gccataaata tacccatata aatcttataa caaataaaat aagtacaatt
     2221 aaagtagaaa taaaagtcaa ttccaatgcc atattttcat ttttactact aagtcctgta
     2281 tatatacttg gtaacaaaaa accaagcagt gtaaagacaa atccattcaa tacatactcc
     2341 aaaacatacc aagtattgtc tgatattatc cttaatttag tagttgtatt ttgtaaatgc
     2401 tctttttcaa ttccatgagc tatccctgcc attacaacag ctaggattcc agatactcct
     2461 atttcttctg ctacaaaata tacaaaaagt ggagtcatta tttgtataac tattaccatt
     2521 ggaatttctt ccaaattcca cttgtgtaaa ctaagtctta tttttataat tatgtatcct
     2581 aaaatacttc ctactattat tcctccaaaa gctgtaatca taaattgaat ccctgcctct
     2641 tctattgaaa aaactcctgt aactgtggca agtactgcaa ccttaaatgc tactactcca
     2701 gcagcatcat ttaataaaga ttcaccttca agtatactca tcatattttt aggaaaattt
     2761 aatccttgtg taattgattt tactgctact aaatcagttg gagatattac tgctgccaaa
     2821 gcaaatgaaa cagataatgg catcccagga ataagaaaat gtataaagat tccacctgca
     2881 aacacggtta ttaaaactaa accaaatgca agaagcaaaa ttggttttct gagctctaat
     2941 agctcttttc tcgatacatt ttgtccttca ctgaatagta atggagctat gatacatatt
     3001 acaaatattt ctggattaaa tgaaaatgag aatttaaatg gcaaaataga caatataatc
     3061 cctagtacaa tttgatatat tgcaagagga atattactcc actttttgct aaatacagtt
     3121 gaaatcgttg tacaaaccaa taatcccaac aatgttacta aaattgacat aactacctct
     3181 cttttcaata ataatattat agattaaact tatatatcaa ctaacttaat attaacagca
     3241 tgaaatccca tcttaatcaa aaaaattttc agtttattct attattctta ttatatacat
     3301 caaatttaat ttttgaaata tctatactag atgacatgca acaaaatgat ttcttgatat
     3361 ttcttttaaa actggaatct cttttttgca tatttcctta caataagggc atcttgtatg
     3421 aaacttacat ccacttggtg gatttgttgg agatggtatg tctcctttta atataattct
     3481 atttttctta attgtagggt cttgtattgg tactgcgctc ataagcgctt ttgtatatgg
     3541 atgttgagaa tgtaagtaaa gttctccttt agttgcaatt tctattatag aacctagata
     3601 cataacacaa actctatgtg caatatgatt tactacactc aaatcatgag atataaataa
     3661 atatgaaagt ttatatttat cctgtaaatc cataagtaaa tttataattt gagattgaat
     3721 agaaacatct agtgcagaaa caggttcatc acaaactata aattctggat ttaatgctaa
     3781 tgctcttgct atacctattc tctgtctttg acctccagaa aactcacctg gttttctatt
     3841 taaatggtac ttagaaagac cacaactttc tacaacattt aatatattat cttttatttc
     3901 attctttggc actaaattat gttctataac tgcctcggat attatttgtt ccacatttaa
     3961 tcttggattt aatgaactat atgggtcttg aaatatcatt tgcattttgg gtcttaactt
     4021 tctaagttct tccttctcta acttatatat atctacacca ttaaattcaa ctttaccttc
     4081 agttttttca attaatctaa gaatggttct tcctgcagtt gattttccac atcccgattc
     4141 tccaactata gccaaagttt ctcctttttt taaatcaaaa gaaattccat ctactgcctt
     4201 tacacattta atttccttat caaataatcc agatttaact tcaaagtact ttttaagacc
     4261 ttctactttt aataaactct cttctttttt caattatatc tcctcctttt tatataacca
     4321 acatgcaata ctatgaccac tattcaattc tattaaagga ggtatctgag ttctacattt
     4381 atcacatact ttttcacatc tatcactaaa ataacatgaa tctggcatcc caattggatt
     4441 tggtacttgt cctggaatcg aatacaatct tttatctttg ttttttccaa gagcaggctt
     4501 tgattttaga agtccaatag tataaggatg ttttggattt ttaaataatt caacaacctc
     4561 tgccacttct ataattttgc cagcatacat cactaatact ttgtcacaca tttctgcaac
     4621 tactcccaaa tcatgtgtaa tcatcattat agatgtgttt aatttttctt ttatattttt
     4681 cattatatct aaaatctgag cttgaatagt aacatctaaa gctgttgttg gttcatcagc
     4741 tataagtaat tttggattac atgataaagc catagctatc attattcgtt gtctcattcc
     4801 acctgataat tcatgtggat atgaattata tatttcttca gctcttggta tttctaccag
     4861 ttttatcata tcgattacct ttttcttagc ttcttccttt gataaatctt gatgtatcat
     4921 aataccttca atgatttgac ttccaatagt aaacgctggg tttaaagatg tcattggttc
     4981 ttgaaaaatc attgatatct cattccctct tattcccata agttcatctt cattcatctt
     5041 taaaatatct ttgcctcgaa agattatttc tccaccttct atttttgatg ttggaagtaa
     5101 cttcatcaaa gacatggctg ttacagactt accacatcct gattctccaa ctattccaat
     5161 agtttcacct tcatttatat taaaacttac ttcatttaca ctctttacaa tccctttatc
     5221 tgtatgaaaa taaactttta aatctttaac ctcaattagt gctttactca ttattttctc
     5281 ctctcagcaa aattacttaa gataacatac accttttgat tgtattttat aaaaatttaa
     5341 attacaacat tttcttacat tttattattt ttttctatta attataataa tataatagtt
     5401 aatttctata tatattgatt taataatgat tactattata caataaaaaa tatgtgttaa
     5461 atattatttt atggaggttt tataagaata tgaaatttaa aaaattggca tcattaatct
     5521 tagtttcatc attaatgctt acatttacag cctgtgcttc caatgataaa gacaagtcta
     5581 gtggaagcaa atctggtgga actgttgtaa tacctatggc aagtgaccca gatataatca
     5641 atggagcttt tgcaaacgtt aaagaagact ctcttgcatc aaatatagtt tatgctcctc
     5701 tttatactta tgaaaaagga aatttagtaa attatttagc agaaaaagta gattttaaag
     5761 atagtaagga attaactata aaattaaaat ccaatttaaa atggcatgat ggaaagccaa
     5821 taactgctga agatgtctta tttacattta atacagtttt agacgaaaaa caaaattcac
     5881 catcaagaca atacttattg gtgggtgaga aaccagttaa agtagaaaaa attgatgatt
     5941 taacagttaa gattacttta ccaactgcaa gtgaatcttt cttatatggt atatctaaaa
     6001 tttctccaat accaaaacat gtgtttgaag gagaatcaaa tatagctaag tcagaaaaaa
     6061 ataataatcc agttggttct ggtgcattca agtttaagga atggaaaaaa ggtgaaagca
     6121 tagtatttga aaagaatgca gattattttg gtggagaatc taaggcagat tctatagcgc
     6181 taaaaataat tcctaatgaa gcatctcaag aagcagcttt aaataatggc gaaatttcac
     6241 ttatgaagac atctgctgaa ggatatgaaa aggcaaaatc aaactcaaat ttacaaactt
     6301 atacttatag tgaagaaaga ttaaactaca ttgtatttaa ccaaaatata tcaaacatgg
     6361 ctaacaaaga agtaagacaa gccttatcat atgccttaaa tagaaatgaa atgatagaat
     6421 cagcatatgg aaaagaaggt tctgtacctg ctaaatctat attagttcca gaagcagact
     6481 tctatacaga agaaggtgtt gaaggatatg accaagatac aaataaggct aaagacttat
     6541 tggataaaag tggtgttaaa attgataaat taaaaatagg ttataacaca ggtagatttg
     6601 ggcataaaaa ctatgcctta gtagctcaac aagagttgaa aaaaattggt atagaagctg
     6661 aaatagttcc ttatgaaagt aaggcattct tcaatatact atttagtaat agcactgaat
     6721 gtgatatgta tgtaaatggt tatgcttggg gattagaacc aaacccatat agaggtatgt
     6781 ttgaaactgg tcaatattgc aaccaaacta aatattctaa tgctgaaata gatgctttat
     6841 gggaaaaagg atttactgag ttaaacaagg aaaaacgtga agaaatatat aagcaaatac
     6901 aacaggatat atctaaggat gcgcctattt acactataga ttatgaacaa aatttaatgg
     6961 cagctcaaaa aaatctaaaa ggaataaaag atgctaaacc atctcctgca atattatttg
     7021 aagattggtc taaactgtat gtagaataat tgatgtaaaa ttttatatta ttaattagaa
     7081 aaggagatgt ctaaaaataa tattgtttta ttattttaaa acgagacatc tcttttaatt
     7141 aggagaattt acttatgtta aaattaatat tgaatagagt aaaagtttta atacctatgt
     7201 taatcttaat atctatatta tcatttggac ttttagaatt agctcctggt gacccagcag
     7261 atgcatatat aaatccttta atgtcttcac aggatattga aaatataagg gttaatatgg
     7321 ggcttgataa accagcatat ataagatatt taaactggtt gaaaaacact ttaagtggaa
     7381 acttaggtat ttcttatata aaccatatgc ccgttacaga acaaattatg gaaaaaatgg
     7441 gaaatacatt tattcttatg ggtacttctt tgatattttc aatattggta gccatacctc
     7501 ttggaatatt tttagctgta aataaaaatt caataactag taaagtatca tctgttttta
     7561 attatatagg tgtttctata ccttcttttt ggataggtat gatactcatt tcaatttttt
     7621 cagttaaact taacattttc ccatcaggag gtatgcatac tattggaaat gatagtattg
     7681 gagatttagc aaaacattta gttttgcctg taataacttt aggtctatat aatactgcta
     7741 tttttacaaa ttatgtagaa gctagtgtaa atgaacaatt aaaaaaacaa tatgttatta
     7801 cagcaagagc aaaaggatta tcagaaaaag tcatactatt taaacatgtc ttgaaaaatt
     7861 cactgacatc attggttaca atcctaggca tgagtataca aaaattagtc actggtgcat
     7921 ttgttacaga agtagttttc tcttggccag gtatgggaag attaatgatt gattcaattt
     7981 tttcaagaga ttatactgta attatggcaa taactatgtt atcagcacta tttttaattt
     8041 taggtaattt agttgctgat atactatact tattaattga ccctaaaata aaatctagca
     8101 aaggaggatt ctagttgtgt ttacaagtat cttaaaaaaa ttaaaaaaag ataaatcctt
     8161 tgcttttgca tgtattactc ttagcataat aatcatatta attatagctt cttttatatt
     8221 aaatatagac tctaatgcaa ttgatactac ttctaaactt caagaaccta gcttaaagca
     8281 tatctttggt acagacgaac ttggtagaga ttacttctct agagctcttt atggtggaag
     8341 gatttcactt agtgttggta ttttatcaat gcttatttct gtagtatttg gtactgctgt
     8401 aggtgttatt agtggatata ttggaggtaa gctagaccaa ttcttgatga gattagtgga
     8461 tatgcttatg agtataccaa gtttcttagt tttgattgta ttaaatactt atctaacacc
     8521 taaagtttcc actattatac taattattgg attcttaagt tggatggaag tttcaagaat
     8581 tgtacgtggc gaaacattaa aaataaagga aaatgaatac tgtctagctg ctaaagctac
     8641 tggtatcaaa actagaaata tactattaag acatatagtt ttaaacttaa aagagactat
     8701 tgtagttgct ggttcgttaa atattgctaa tgctatctta actgaatctg ctcttagttt
     8761 tttaggtctt ggaatccaat tgcctcttgc atcatggggt aatatgcttc aagatgctca
     8821 aagacatatt tttgacaagt tatatttagc tgtatttcca ggattactta tatttttaac
     8881 agtcatgagt ttgactataa taagcaaaaa attttctagt aatacttaac aaattttata
     8941 tagaaaataa aaacagtgat tatctaaaga tatacactgt ttttattttt cgtttaaaaa
     9001 aatacatatt ttgtatatta taactttaaa tactactcta ccatgttatg caaagcgtat
     9061 tattgctctc aaacatatag atgaaaaact aatagtttat tataataaaa attgatttat
     9121 ttatttgtta ttttaataac atttttaaga gttcttttta ctaaagaaat ctataataaa
     9181 tttaaataca tgttggtcta catggctata acgttctttt ttaggtctac ataatataac
     9241 tttccatgta ataggatgat taaaaggctt ttctataatt ttgctatttt caaaaaaatc
     9301 atgaactggt gatggtaaaa ttgttataaa atcagaattt gtggttgata gcaacaaaaa
     9361 atcccatgaa ccagacatta tatatctttt taacttaaca ttttcatcag taaatttttg
     9421 cattagctta tgatgtatca taaaagtagg attaaatatt gcaagtaact gctcattcaa
     9481 atcgttccaa tctatcttat ccttcttagc aagtggatga tttatattta aaaatgctgt
     9541 aagttcatct tcttgaagca catgttcagt tactacactt ttatcaattt tatgtggatg
     9601 taacaatact gcataatcta attcctgtaa gataagcatt ctatttaagt catatgcacc
     9661 cttctctact atatcaaatt ctatatctgg attatttgca actaactgtg caactacatc
     9721 tgaaaatgct attccaagta taagtggagg tattcctatt ctaacttttc ctttaaattg
     9781 aactgaatct tctcttaaat cttccatcat atttttgtat tcatttatga tatgttctgc
     9841 atttacataa aatctttctc ctgctggtgt taacccattt aacctgcctt tatacctttc
     9901 aaataaatat acatcttcac tttcttcaaa attttttata atttgactta atgctggttg
     9961 agatatgtga agtgtcttag atgctattga taaattaaaa ccagatttca ctatttcaac
    10021 aaaatatttc aactgtttta tatccatttt aaaccccctt ccccaaaagt ataatttaga
    10081 ttatattttt tcttctaatg atattttata ggaaaatcaa cataatttca ttaatatttt
    10141 aacaaaatat ttttctaaat ctactataaa tttttatcct ataaattttt cttatacact
    10201 atataaaaaa ttagacttag acaaaacgct tttttcgccg taatctataa ttagaaacag
    10261 gaaaacctaa agcattagga ataaaagtaa ttgaaatact taaaaacaag cttgtattaa
    10321 aaaagtactt tattatagtg ttccctaatt tctttattca gaaaattttt aattatcatt
    10381 caagaaaggt aactattaga tatgaaagat gtagtaatcg tatcagcagt cagaacacct
    10441 ataggaagtt ttggaggtgt atttaaaaat acttcagctg ttcaacttgg aactatcgct
    10501 gttaaagaag cgatatcacg tgtaagactt aatttatctg aaatagatga agtaataatt
    10561 ggaaatgttc ttcaaacagg gcttggacag aatgttgcta gacaaattgc tataaatgca
    10621 ggtataccaa attctgttcc aagctacaca gtaaataaat tatgtggttc agggcttaaa
    10681 agtgtccagc tagctgccca atcaatcaca tctggtgaaa atgatgtggt aattgcaggt
    10741 ggtactgaaa atatgagcca agctccttat atcgttccaa ctgctcgctt tggtagtaaa
    10801 atgggtgaca taaccatggt agatagtatg ctaacagatg gccttattga tgctttcaat
    10861 cagtatcata tgggaataac tgcagaaaat attgcaacta aatttgaatt tactagagaa
    10921 atgcaagata aattggcatt agaaagtcaa aataaggctg agaatgctat aaaaaacaac
    10981 cgctttaaag aagaaatcgt tccagtagat gttttaatac gtcgaggaaa aatagaaact
    11041 attgataaag atgaatatcc taaattaggt atgacatttg aaggtttatc taaacttaaa
    11101 cctgcattca aaaaagatgg tactgtaaca gctggaaatg cctcaggtat taatgatggt
    11161 gcggctatgt taatactaat gagccaacaa aaagcagacg aactaggtat tagaccactt
    11221 gctaaaataa aatcctatgc ttcagctggt gttgaacctg aagtcatggg tactggtcca
    11281 atccctgcaa ctagaaaagc actgaaaaaa gctggactta gcatcaatga tatagactta
    11341 atagaagcaa atgaagcgtt tgctgcccaa gcattagctg ttaaaaatga acttcaaata
    11401 gattcatcaa agttaaatgt taatggtggt gcaatagcat taggacatcc aataggagca
    11461 agtggagcta gaatacttgt taccttaatt tatgaaatgc aaaaacgtaa agttgaaact
    11521 ggacttgcaa cactttgtat tggtggtggt caaggaatat caatggtggt ttcacgatag
    11581 gaggaaatat gaataaaata gttagcattg atgaagctct atctcatgta aaagatggag
    11641 atacaataat ggttggtgga ttcatggcca atggttcacc agaaaattta attgattatt
    11701 tatgtagtaa aaatataaaa gatttgacac taatttgcaa tgatactggc tttattgaca
    11761 aaggagttgg caaaatggtt gtaaataagc aatttaaaaa gataattgct tctcatgtag
    11821 gattaaataa agaaactgga agacaaatga ataacaatga aacagatgtt gagttagttc
    11881 ctcagggaac tcttgctgaa caaatacgtg cagctggtta tggtctagga ggtatactca
    11941 caccgactgg tattggtact cttgttgagg agggaaagca aaaaattgtt gtcgatagca
    12001 aagaatacct tcttgaaaaa ccaatttatg cagatgttgc tcttctattt gcatcaaagg
    12061 tcgataaagc aggtaatcta gtttataaag gctctatgaa caattttaat aatttaatgg
    12121 catctgctgc caaaattaca atagttgaag cagatgaaat cgttgaaatt ggtgatattg
    12181 acccaaatga agttaataca ccaggtattt ttgtgaatta catcgttgaa ggaggtaatt
    12241 tataatggat aaattagaaa tgcaagaata tattgcaaat agagtatcta aagaattaaa
    12301 agacggagcc gttgtaaacc ttggaatagg tctaccaacc aaagtagcaa attatattcc
    12361 ggataatgta aacgttatct tacaatctga aaatggcttt ttaggtttag gttcagccga
    12421 agatggaaat tcaagtgatg aaactattgt aaatgcaggt ggtcaaccag ttactatact
    12481 tcctggagga tgtttctttg atagtgctac ttcttttgga ataatcagag gaggtcatgt
    12541 tgatataact gttcttggcg cacttcaggt tgacaaatat ggcaatatag caaactatat
    12601 gattccaggt aagatggttc caggtatggg tggagctatg gacctagtaa ctggagctaa
    12661 aaaagttatt gtagcaatgg aacatacttc aaaaggttct gcaaaaatat taaataattg
    12721 tactcttcca cttactgcaa caaatgctgt agatttgata gttactgaaa tgggagttat
    12781 ggaagtaaca tcagatggaa ttttacttaa agaaataaat ccagctttca cattagatga
    12841 tgttatttct gctactgaag ctcctttaat attgtcagat tcattaaatg gaaaaatatc
    12901 tgttgtttaa aataattaaa actagaaaat aaaaatctct tagacgtatt acatataaat
    12961 ttgtattgct taaaatatta atataaaaat aaaataaaat tggaggaaat gaaaatggtt
    13021 aaagataaag ttgtattcgt aacaggagct gcaagtggaa taggtaaaca aatagctgaa
    13081 tcgttcttaa aaaatggtgc taaagtaatg ttctctgata taaatcaaga agctttaaat
    13141 aataccataa cagagttaca aaaaaaaggt tatgattgca tgagtgtaaa gtgtgatgta
    13201 actaaggaag aagaaattaa ccacgctata gacaaaactg ttgaaaagta tggcagatta
    13261 gatatattaa taaacaatgc tggacttcaa catgtttcta tgatagaaga tttcccaact
    13321 gataaatttg aatttatgat aaaaataatg ctgacagcac cttttatagc tactaaaaaa
    13381 gcatttccta taatgaaaaa acaaggcttt ggtcgtataa taaatatggc ctcaatcaat
    13441 ggtgtaatag gatttgctgg aaaggctgct tataactcag caaaacatgg acttattgga
    13501 ctaacaaaag ttgctgctct tgaagctgcc gattctggta taacagttaa tgctttatgc
    13561 cctggatatg tagatactcc acttgtcaga ggtcagttca atgacctttc aaaaactcgt
    13621 aatataccat tggaaaatgt cttagaggaa gttttatacc cacttgtacc acaaaaacgc
    13681 ctattagatg ttcaagaaat tgccgactat gctatgttct tagcaagtga taatgctaag
    13741 ggtattacag gacaagcttg cttactagat ggaggatata ctgcacaata gttattatat
    13801 ttagggcttg aagaaagtat tcttcaggtc cttctactaa ggagggatat ttatgagtat
    13861 tatcgcgcca attggtataa ttattggtat catagctatt atatattttt cagttaaaga
    13921 aattcatata actatagctg ctccaatagc aactttaata gttgttattt taaataaaat
    13981 ggatgttgta acgtcaatgc taggtgctga caaaaacaat tatatgggtg cacttggaaa
    14041 ctatataatg agttattttg caatattctt attaggctca atacttgcta aatttatgga
    14101 agaaagtgga gctactgtat caatagctga ttttatttta aataaatttg gctctaatca
    14161 tccttataga gttttagttg ctatatttat agtaagtttt atattaacgt atggtggtat
    14221 aagcttattt gtagttatgt ttgcggtcat tccacttgca agaactctat ttaaaaaact
    14281 tgatatttct tggaacctta ttcagatacc actttggctt ggtattgcaa caattactat
    14341 gactataatt cctggaacac cagccattca aaatgttata cctattcaat atctaaatac
    14401 gtctcttact gcagctacta taccaagtat tgtaggtagt ataggttgtg ctacatttgg
    14461 acttgtctat atgaaatatg ctctaaacaa aagtattaga aaaggtgaaa cttatgcaac
    14521 ttacacttca gaattagaag aagaatcttt agatagagaa ttaccaggat tttttgcaag
    14581 tatttttcct ctattagcac tcgtaattat agctataact ggaagtactt ttggttcaga
    14641 atttatgaaa aaaaatgtaa tttacatagc tttaatagtt gcaattcttt tatctgcttt
    14701 tttattccat aaatttctat catctaagat acagacattg agcataggtg ctagtggttc
    14761 tataggtcct atatttgcta catcttctgc agttgcattt ggagcagtaa taatggctgc
    14821 acctggtttt gatgtattta gcaaacttat aatgtcaata ccaggtagtc cacttataag
    14881 tcttactgtt ttgacttcaa caatgtcagc aataacaggg tcctcttctg gtgcattagg
    14941 aatagtaatg ccaaactttg cagattatta tttaagtact ggtctaaatc cagaattaat
    15001 tcaccgtgtt gctacaatag cttcaaatat actaactata gttccacaaa gtggtgtttt
    15061 tcttacattc ttaagcttat ctaagttaac acataaaact ggatttaaag aaactttcat
    15121 tgtagttgca gttggttcac taatagcaga aattattgta attacattag gattactaat
    15181 gtattaatat aaaacaagtt aaaattcata atatgaaagg tggtgtctca aagtgaactt
    15241 tttacttaat gagacactct ttttatatga aataacatat gcctatcatt taaacaataa
    15301 aaaaggcctt atctctattt tgagacagac cctttttatt tcttaaagat ttaaataatt
    15361 ataatttgtt gctaatacga aagcatctac tgaataaatt taaccaaata aaaaaaaatt
    15421 tatcataact ataaatttac gttatggaat tattataaaa atattatttt ctctaactaa
    15481 gtaattccat actatacagg cttttaatac aaattgtcaa acacactgaa atatacctat
    15541 ttattccata tatatctttt ttacatatct ttacagtaaa aaaagtctta atcttatgtt
    15601 agtatagatt cacgggtaac cccctacact aaaggttaca aatcagtaac atttttagaa
    15661 tttttagatt aagaggtgtt taatgatgag aagaaatata ccaacaaaat taaaaactat
    15721 attagcttta ggagtgttaa ctttagcaac agcttcaagt gctgttactg caagtgcttc
    15781 atcacttgaa aataatgtaa atataagtaa caaaagttta gtatatagta atactaaata
    15841 caagttttct gcaaaaccaa attgttctgt aaaaccagaa cttaagcctg ataaagataa
    15901 ctcttgtaag gataagaatc ataatgacaa gaattgcaac aacactaata aacctgagga
    15961 caacaataat tcagactcta ctaataagcc tgataacaat aaacctgagt ctaataaacc
    16021 tgaagacaac aataattcag gctctactaa taaacctaat aataataaac ctgagtctaa
    16081 taaacctgaa gacaacaata attcaggctc tactgataaa cctgataaca ataaacctga
    16141 gtctaataaa cctgaagaca acaataacaa taattcagac tctactaaca aacctgatga
    16201 taataacaac tcaggctcta caagtgaaag tttttcagct taccaaaaag aagttgttga
    16261 cttagtaaat atagaaagag ctaaagctgg attaaatcct cttactcttg attcttctat
    16321 atcaaatgtt gctacaaaaa aatctcaaga tatgattgat aacaactatt tttcacacaa
    16381 ttctccaact tatggttctc catttgacat gttgaaaaaa tttggaataa gctataagac
    16441 agcaggtgaa aatatagcta tgggtcaaaa aactcctaaa gaagttgtaa acgcatggat
    16501 gaattctgaa ggtcacagaa aaaatataat gaatccaaat ttcagtaaaa taggtgttgg
    16561 agtagcacaa aaaagtggtg gttcaattta ttggacacaa atctttgtag gataaaatac
    16621 tctttttcca tacatatcaa aagagtatct ctaagtgata aaaaacacaa atgagatact
    16681 cttttgtatc tattttaaaa ttatcaaatc caaatataca tactataaat attatttgtt
    16741 ctcttaattt tagtgtgtag aacaatatat aaaaagtatt ggcaagatga aaatggaaaa
    16801 cttaaaaaag atatttaata ggtaaaaaca aaatcttaat atcttcataa aaaaaggctg
    16861 tctcaaaata gagataaggc ctttttcatt gttgaaatga ttaaaatata tttgatttca
    16921 tacaaaaaga gtgcctcatg aactaaaaag ctcactttga gacactctct tttatcaatt
    16981 tatataagca ttataatact ttaatattat tcagctaatt ttttgtatcc ttcatatctt
    17041 tctttagcat cagcttcagc tttagcaaat aatccatcag cttcttctgg gaattgttta
    17101 gctatagcag aatatcttac ttgtgcaagt atgaaatctt tgaagcttgc agttggttct
    17161 tttgaatcta aagtgaatgg gtttttacct tcagctttta cttctggatt gaatctgtat
    17221 aaatgccagt atccgcaagc aacagcttgt gcttcgttag ctacagttct tcccatacct
    17281 tcttttaatc catggtttat acatggagca taagctatta ttagagatgg cccatcatgt
    17341 ttttcagctt ctataacggc tttgataaat tggtttttat cagcacccat agcaacttga
    17401 gctacatata cgtttccata tgtcatagcc atcattccta aatctttctt tctagatttt
    17461 ttacctgaag cagcaaactt agccatagca gcagctggag tagctttaga agcttgacct
    17521 cctgtatttg aatatacttc tgtatcaaat acaagtacgt ttacatcttc accagatgca
    17581 agaacatggt ctaatccacc gtaaccgata tcatatgccc aaccgtctcc acctagtatc
    17641 cattgagatc tcttaactag ataatctttt ctatctttta tagcttttac taactcttta
    17701 gctttttcat ctgtacaatc acaagctggt ttttctaata attcaacaac cttagcactt
    17761 gctactttag aagcttctcc atcatttcta gaatctaacc actctgtaaa tactgcttta
    17821 gcatcttcac agatacccat agaaactaac tctgtcatag catctactat tttatttctt
    17881 atttgcttaa cagctgtgta cataccaaat ccatattcag cattatcctc gaataaagag
    17941 tttgcccaag atggaccttt accttcatgg ttacaagtat aaggaattga tggtgcagaa
    18001 gctccccata tagaagaaca tccagtagca ttagctatca tcattctatc tccatataat
    18061 tgtgtaacta ctttaatgta tgcagtttct ccacatccag cacatgctcc tgaaaactcc
    18121 attaatggtt gtctaaattg acttccttta acagtgttta caggcataac atcacctttt
    18181 ggagctacct catcaggatt tactgcaaat gcccaattat ctaactctgt atcaagttga
    18241 gtatctattg gcttcataac taaagctttt tctttagctg gacatatgtc agcacagttt
    18301 ccacatcctg tacagtcaag tggactaagt tgcattctat attttaatcc ttctaatcct
    18361 ttacctaatg cttttttagc ttcaaatcct tctggagcat tagctaattc ttcttcagta
    18421 actaatactg gtcttataca tgaatgagga catataaaag aacattggtt acattgaata
    18481 cagttatcta ttatccattc aggaacatca acagctatac cacgtttttc ataagctgca
    18541 gtaccacaag ggaatgttcc atcttctata ccattgaatg tacttactgg taagttgttt
    18601 ccttcttgtg cagtcattgg tcttagtata ttttttataa attctggttc tttaacttct
    18661 cctttatctg catcaacagc attttcccaa gaagctggaa cttcaacttt aactaaagca
    18721 tttattcctg catcaacagc agcatagttc atgttaacta ttttttcacc ttttttacca
    18781 taagctttaa ctatagaatc ttttaagtat ttaacagcat cttcttctgg tattatttca
    18841 gctaatttaa agaatgcaga ttgcattatc atatttattc tgtttccaag acctatttct
    18901 tgtcctagtt taacagcgtt aactgtatag aattttatat catttttagc tatatattgc
    18961 ttcatatgag ctggtaaatg ttcttcaagt cctgcttggt cccatatagt gttaagtaca
    19021 aatgtaccac cttttttaag accttttaat aaatcatatt gataaacata tgattgttta
    19081 tgacaggcaa tataatcagc ttcatctatt aaataagtag ctcttattgg agtatcacca
    19141 aatcttaagt gagacatagt tataccacca gattttttag agtcataatc aaagtaagct
    19201 tgagcatatt tttcagtatg gtcaccaatt attttgatag cttgtttatt agctccaaca
    19261 gtaccgtcag aacctaatcc ccagaactta catcttatag ttcctttaga agctattttt
    19321 attggctcag atggagttaa agaagtatta gttacatcat caactatacc tactgtaaat
    19381 ccatttttag gttgttctga aactaagtta tcaaaaacag tttttatatc tgaaggagta
    19441 gtatcttttg aacctaatcc gtatcttcca cctattatca taggagcatt ttcttttcca
    19501 tagaatacat cacgtacatc taagtataat ggttcaccag ttgaacctgg ctctttagtt
    19561 ctatcaagta cacatattct ttcaacagta gatggcatag catctaagaa atgtttcatt
    19621 gagaatggtc tatataaatg aactttaact aaaccatatt tttctccctt agcatttaag
    19681 taatctatag tttcttctat tgtttctgtt actgaaccca tagctattaa tacgtattta
    19741 gcatcttctg ctccatagta atcaaataaa ctatgttttc tacctgttaa attactcatt
    19801 ttttccatgt atttttcaac tatacctact atattttgat agtatttatt agaagcttct
    19861 ctagtttgga aatatatatc tgggttttga gcagtaccac gagcaactgg atgatttgga
    19921 gataaagcat tatctctaaa tgcttgaact gcttcaaagt ttaataaact tgcataatct
    19981 tcattttcta gtaattcaac tttttggatt tcatgtgaag ttctgaatcc atcaaagaaa
    20041 tgtataaatg gtaatctacc ttctattgca gctaaatgtg caacaggtgc tatatcagct
    20101 acttcttgaa ctgaaccaga agctaataat acacatccag tttgtctagc agccataacg
    20161 tcttggtgat ccccaaatat tgataacgct tgagatgcta aagcacgagc acttacatgg
    20221 aatactcctg gtaataactc tcctgcaact ttatacatgt taggtatcat taataataaa
    20281 ccttgagatg ctgtaaaagt agaagttaaa gctcctcctt gtaaagaacc gtggaatgtt
    20341 cctgatgcac ctgcttctga ctgcatttca acaacattaa ctgtttgtcc aaatatattt
    20401 tttcttcctt gtgatgccca ctcatcaact acttcagcca tagttgaaga tggtgtgatt
    20461 ggatatatag ctgctacatc tgtaaacgca taggcaacat gagctgcagc tgtatttcca
    20521 tcaagtgttt tcataaactt agccataata tgtatcctcc ttgatagcta caattgtact
    20581 tgtttataac aagtctattt ttttatattt acatataatt tacacaacaa aactataaac
    20641 acacatatag tcgcgcaaat actctatctt ctaatataat actacagatt agataaaatt
    20701 cctttgaaaa ttgtaaatta attacatatt taagtagatt ttttttaaaa ttaaatatgt
    20761 taagtttt
//
LOCUS       DAMJZE010000055        20718 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJZE010000055 DAMJZE010000000
VERSION     DAMJZE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.055"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="PC541_001774"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="PC541_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2839240.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="PC541_001775"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="PC541_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2839241.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="PC541_001776"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="PC541_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2839242.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="PC541_001777"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="PC541_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2839243.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="PC541_001778"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="PC541_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2839244.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="PC541_001779"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="PC541_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2839245.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="PC541_001780"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="PC541_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2839246.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="PC541_001781"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="PC541_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2839247.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="PC541_001782"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="PC541_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2839248.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="PC541_001783"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="PC541_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2839249.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC541_001784"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC541_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2839250.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="PC541_001785"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="PC541_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2839251.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="PC541_001786"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="PC541_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2839252.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="PC541_001787"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="PC541_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2839253.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="PC541_001788"
     CDS             6989..7174
                     /locus_tag="PC541_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2839254.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="PC541_001789"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="PC541_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2839255.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="PC541_001790"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="PC541_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2839256.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="PC541_001791"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="PC541_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2839257.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="PC541_001792"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="PC541_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2839258.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="PC541_001793"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="PC541_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2839259.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="PC541_001794"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="PC541_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2839260.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="PC541_001795"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="PC541_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2839261.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="PC541_001796"
     CDS             11117..11767
                     /locus_tag="PC541_001796"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2839262.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="PC541_001797"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="PC541_001797"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2839263.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="PC541_001798"
     CDS             12536..12805
                     /locus_tag="PC541_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2839264.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="PC541_001799"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="PC541_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2839265.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC541_001800"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC541_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2839266.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="PC541_001801"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="PC541_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2839267.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="PC541_001802"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="PC541_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2839268.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="PC541_001803"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="PC541_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2839269.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="PC541_001804"
     CDS             14901..15848
                     /locus_tag="PC541_001804"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2839270.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="PC541_001805"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="PC541_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2839271.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="PC541_001806"
     CDS             16330..17163
                     /locus_tag="PC541_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2839272.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="PC541_001807"
     CDS             17151..18017
                     /locus_tag="PC541_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2839273.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="PC541_001808"
     CDS             18011..18814
                     /locus_tag="PC541_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2839274.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="PC541_001809"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="PC541_001809"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2839275.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="PC541_001810"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="PC541_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2839276.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="PC541_001811"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="PC541_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2839277.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8272 a         2446 c         4321 g         5679 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaa
//
LOCUS       DAMJZE010000056        20715 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJZE010000056 DAMJZE010000000
VERSION     DAMJZE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.056"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            70..1947
                     /locus_tag="PC541_001812"
     CDS             70..1947
                     /locus_tag="PC541_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2839278.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2189..4102
                     /locus_tag="PC541_001813"
     CDS             2189..4102
                     /locus_tag="PC541_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839279.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4095..6314
                     /locus_tag="PC541_001814"
     CDS             4095..6314
                     /locus_tag="PC541_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2839280.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6423..7253
                     /locus_tag="PC541_001815"
     CDS             6423..7253
                     /locus_tag="PC541_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2839281.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7243..10710
                     /gene="addB"
                     /locus_tag="PC541_001816"
     CDS             7243..10710
                     /gene="addB"
                     /locus_tag="PC541_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2839282.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10710..14537
                     /gene="addA"
                     /locus_tag="PC541_001817"
     CDS             10710..14537
                     /gene="addA"
                     /locus_tag="PC541_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2839283.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14583..15794
                     /locus_tag="PC541_001818"
     CDS             14583..15794
                     /locus_tag="PC541_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2839284.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15781..19308
                     /locus_tag="PC541_001819"
     CDS             15781..19308
                     /locus_tag="PC541_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2839285.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19439..20692
                     /locus_tag="PC541_001820"
     CDS             19439..20692
                     /locus_tag="PC541_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2839286.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8988 a         1957 c         3680 g         6090 t
ORIGIN      
        1 gcatttgcta ctgaatctaa tgctatggaa aataacgctg atttaaatat aaacttagag
       61 aaaaaaagta tcgttttagg tagcaaatca aaagttagtg tcaaatttaa agaaaaacca
      121 gatgcagata gcattacatt aaagtataaa tgctatgaca tgccattgaa tacaactcta
      181 aattacaatc aatcaactgg ggcatatgaa ggaactatca attataacca agacccagaa
      241 tatctaaatg tttgggaact acaagggata acaataaaca gcaaaaataa tcctaaaact
      301 ttaaacagac aagacctaga aaagctggga ttaaatttaa aagactataa tgtaacacag
      361 gaatgtataa ttgaagatat aacttctaga aaagatgtaa ataaatattt gagaaaaact
      421 tcttcaccta ttacagaact tacaggaagt gatagatatg aaacagcagt taaaataagt
      481 aaagagggct ggaaaaatgg ttcagataag gtagttataa taaatgggga tgtaagtata
      541 gatggcatta tatcaactcc actggcaacc acatataatg caccaatact tttggttgaa
      601 aaaaacaatg tacctaatag tgtaaaatca gaattaaagc gcctaaaccc taaagatata
      661 attataattg gagatgagaa tgctatttct aaaactactg ctaatcaaat taaatcaact
      721 gtaaatgcta gtcaaacacg tttaaatggt tctaatagat atgagacatc tttattgata
      781 gcaaaggaaa tagataaaaa tcatgatgtg gaaaaagtat acataacaaa tgctaatggc
      841 ggagaagtgg atgcacttac tatagcagca aaagcaggtc aagacaagca accaattata
      901 ttaactgata aagatagtat tacagacaat acatataaat ggttaaagag tgaggattta
      961 caaaatgctt attttatagg tggtcctcaa atgatatcaa caaatgttat aaataaggta
     1021 aatggaataa ctaaagatag tgttactaat aatagagtat acggagcaga tagacacgaa
     1081 acaaatgcaa acgtaataaa aaaattctat acagatgatg agttagaggc tgttttagta
     1141 gctaaatcag atgtacttgt tgatgcttta gcagcaggtc cattggctgc gaacctaaag
     1201 tctccaatac ttataacacc aaagacgtat gtatctgcat accataaaga taatttagaa
     1261 gctaaatcag ctaataaggt atacaaaata ggaggaggat tgacttctaa ggtaatgagc
     1321 tctatagcat catcattatc taaacacaat acgactccaa cagaaccagg aaatagtggg
     1381 ggcaagacag ttatgattga cccagggcat ggtggttcag cacctggaaa ttcatctgga
     1441 ggaatgattg aaaaagatta caatttaaat acttcacttg caacaactga atatttacgt
     1501 tcaaagggat tcaatgtaat aatgacaaga gacacagata agactttatc tcttggaaat
     1561 agaactgctc tatctaattc attgaaacca gatttattta caagtataca ttataatggc
     1621 tcaactaata aacaaggtca tggtgtagaa gtattttata agcttaaaga taaaaatgga
     1681 gggactacta aaactgtagc taccaatata ttaaatagaa ttttagagaa atttaaactt
     1741 acaaatagag gtataaaaac aagagtactt cctagtgatt ctacaaaaga ttatttatac
     1801 gttttaagaa gtaatgatat gccagctgta cttgtagaat gtgcattttt ggataatgaa
     1861 aatgatatga gtttaataaa ctcatctgca aaagtaaaag aaatgggtac acaaataggt
     1921 aaaggaatag aagattcatt aaaataaatg gaaataaaaa tatttaaaaa cttaatgtaa
     1981 atttatagtt gtaaatttat actaaagtca aaaaatgaaa tataagtgta tcaatagtaa
     2041 gactgtatgt acatttgcac gtgtgaatgc aagtatatat acagtctttt tttgtagtta
     2101 aatgcaataa tttgaatgtg ttttgtaaaa tacacaatat gatataatta gataatatac
     2161 acttaattta attgaggaag gtgattatat ggaatattta tttgaaaaaa gtagtttaaa
     2221 agaatatgga aatgaagctt tagataaata tagagaatta ttagataaaa ctggtaataa
     2281 ttcaaggtgt attcttttgt tagtaccaaa caataacaca aagatacgat atgaaaggtc
     2341 tctaagatta aattatagtg aagaactgaa aataacaaca tatataagtt ttgtgaagaa
     2401 agaattagtt aaatactggc cactaataat agaaaaatgt gatgaaataa gtacaaaggt
     2461 tgtatctcca acatttatat caaatagttt aagtgaatat ttaatagtgc aaaaagttaa
     2521 ggaaaaaagg atacaagaag gatattttga agatattaca ggaaccaata aatccatagc
     2581 aaatagtata atgactaaca taaataagtc tgcatttaat ttaattgatt ttgattttat
     2641 tggagaaaaa atatattcat caaagaaaaa taaagatagt atatatagat tttcatacac
     2701 tcaaatggat gaaattatat catattatat aaattcctta ctatcaaatt caattttaga
     2761 taatgcatta agcatatatt tatacaatca atacctactt aatgatgatt tttatttaaa
     2821 aaacttattt acagaagtga gatatataat tgtggatagc cttgaaagct caagtaatgc
     2881 agaagttgat tttataacag aagcattgga taatacatta caaagttata tatattttga
     2941 ttatagtagg gattattctg tatttaataa tgtggatata gactatataa atgagaaaat
     3001 aatatcaaaa attaagccta aagaagaaaa tattaaaaag aaggagatta gaatagaaga
     3061 tttatactta ttacctgcaa atatagaatt aaatcaatca agtcaattat ataatgaaat
     3121 gttagattta ataagtgaaa aagttatatc tcttatagaa gaagatgtat ctccaagaga
     3181 tatagcaatt atatctccta taaacaatag tatattggag catcaaatta gagattcttt
     3241 gatagaaaaa aatatagatg tttttagtac taagaaggat aaaaaagctg tggattatcc
     3301 atatgggaat gcattggtag tggctacatg tatattttat ggatatttag attttataaa
     3361 agatgatgaa tacttaagct ttttagaaac tcttttagaa ataaatagga taaaagcatt
     3421 taagattttt aatgaaacaa gacatttaag tattgataat atagatgaac atagtcagta
     3481 tcgtgagatt ttacagtata ttgaagaaaa aaagaagagt gatataaaaa tacatgaatt
     3541 tttaactcaa ttttacatag ataaaatgtt aaatttaaaa gaaggtaaaa agaatgttgg
     3601 gttatgtaaa aagataatag ctgaaagtga gtcttttagt gaaagtttaa gtctacttgg
     3661 gatgaaaaaa gaaaagatat ttatagaagc tttaaaaagt acaataaacg attattactc
     3721 tgttgtcgat atagaagagt tgaaatccaa agacaaaata gtaataacta ctccttattc
     3781 atatatatca tcaaatatag atagaagtat acaaatatgg gtagatatag gaagtaatgc
     3841 ttggaatatg aaaatagaaa aagatataag taatttaatt gtacttagaa aatcttttga
     3901 agaaaaaaag atttatacaa atgaaatgga agaatattat aaaaaatact atctatacaa
     3961 tacagtatat aatttgttat taaatgcaaa gaaaatctat gcatttaaaa gtgaatatgc
     4021 gattaatggt tatatccaag agagtatatt atatagtata cttttaaaaa tctcacataa
     4081 aggagataaa aattatgatt aatataaatt atagagaaga ccagatacca ataataaatt
     4141 acgataatgg tactatggca gtaccagcag taccaggtgc tggaaaaaca tttataataa
     4201 caaatttagt agctaaacta ttacttgagc agaaacataa gggtggtaaa atattaattc
     4261 ttacatatat gaatagtgct gtaaataatt ttaaaggtag aataagaaaa attcttgaag
     4321 agaataaaat agatgatact aattcgtatg aagtaatgac tatacacagt ttggctgtaa
     4381 aaataataaa ggaaaagcca gaagttgtaa tgttaagtga ggattttaac atagctgacg
     4441 atttgcaaaa aagtattata ttaaatgaat gtataagtag atttagattt gaaggtggag
     4501 aaagagcttt tagatggttt ttaaaggagc aaaaagatga acgctggaaa gaaataactc
     4561 ttgaagcctg ggaaagaggt ttttttgaat ttgcaggaaa tgcaataagt gaacttaagt
     4621 ataagggaat ttctccagat aaccttgaag aaatattgtc aagaggacac aaaggaatgc
     4681 ttaaaattat tttaccaata tataaattgt atgatagaaa attaaagcaa aatggattgc
     4741 tagattatga tgatatatta attttagcag aaaaaacttt aagcttagac gaagggctta
     4801 gaaagaagtt tcaaactaga tacaaatata tatttgaaga tgagtgtcag gactcaaatg
     4861 aaattcaagg taatataata aaaattatat ctagcgaaaa taaaaaccta gttagagttg
     4921 gagatataaa tcagagtata acaggaacat tttcatcatc agaccctaaa ttttttaaag
     4981 aatttataaa tagtgcagat ttctgttata ggatggatat gtcaaatcga agttcaaaag
     5041 atatacttga tttagcaaac actcttgtca agtatgttac aaaggaattt agacagaaag
     5101 aatgtagaca tgctcttgaa gatatgcaaa taaaaactgt gccaaatgga atgggatata
     5161 aagaaaatcc taatccagaa cattataata taaatgtaaa gtggtatgag agttggaaaa
     5221 ttgaaataga gcaaactgct agatatgtta aaggtataaa aaataaatat ccagataaaa
     5281 gtataggtat attggttcca tttaatgaac aagtgacaca agttgctaaa gaacttagag
     5341 agaataatct aacttttgaa gaattaggac caaattcttt gaataaaagg aaggttataa
     5401 acaacatagc atatattatt gattttatgt tacattgtga tgatgtagaa aagttaatag
     5461 ttgtacttaa taaagttttt atagatactg ataatgaaga agaaataaag tattttttag
     5521 aagaattaaa aaactattct actgaagaaa ttatttataa ttttgaattt agtaagttag
     5581 ataatgattt aatagaattt aaagaagaaa gtgacatata tttaagtttt aaaagaggaa
     5641 tagatattat aaaacagata ttagaatatc caataataag gttagacctc ttagttttgt
     5701 ttattgggaa aaaacttaat ttagaaaaag aagataaagc tgtactggat tatatatctt
     5761 tttatataaa gtatctagta tcagaaaata taaatatgga tttagaagat gtatataatt
     5821 tattgtttga tgtaaagaac agggtgttta atcatataat agatgtggta tatgagataa
     5881 atggatatga accagagccc ggtagtataa cactatgtaa ctatcataaa tcaaagggga
     5941 tggaatggga ttgtgtgttt ttactaggtc ttgttgaata taatttccct gacaatatta
     6001 atcaaaagtt tcagagtgat aaatggtatc taaaagaaaa atataaaaac cctatggcaa
     6061 ttattaaatc agaagttgaa gctatattaa aaggaagtat ttcaacagat tatgctcata
     6121 aaacgaagat tgattctata aatgaaaaga ttagactttt atatgtaggg attactagag
     6181 caaaggaaat gttagtactt tcaggaagtg cttatagaga tgaaagtgat atagggaata
     6241 agagaaaaga gcaaaaacca tgtatatatt taagcagact taatcaacat attattgaaa
     6301 aaaggtcaaa ttaatatata ataagttatt aaaagattgc ttgaaatata tctaaaaggg
     6361 gataaaacaa tatgtacagt aagtaactac taaaaaataa agttaagaga gaagtgatat
     6421 gagtgaacga taaattaaaa tatttttctt atagtcaaaa ttcaattaac acatataaat
     6481 catgtccatt gaaatttaaa tataaatata ttgatagaat aaattggaaa aatgatgatg
     6541 ttggtagtag agagtattat gaaactttaa aaacaggaag agattttcat ttgatttgtg
     6601 aaagatattt tagcaatata cctcttggaa tctattttaa tgaggatgat aaaaatagta
     6661 aaaagttttt aaagtggatg gaaaatataa aaaaagttgt tcctattgaa aaaggaaaaa
     6721 cgtatttgcc agaatatgaa gtaaggatga cattaaatgg ggatataata caggcaaagt
     6781 atgatttagt tgtaattgga aataacaata tagaaatatg ggattggaaa accgagtcta
     6841 aaaagattga ctataaacac gtagaaaata gaatacaaac tattgtttat atgtttttag
     6901 caaaagaagt aatacctaaa gttttaaaaa tggacataaa tgttaaagat ataaatatga
     6961 gatattatca acctgaattt gatgacttac caattaccat tttatatgat gaagaaaaac
     7021 atgaagcaaa tagaaataaa attcaaaatt atataagtat gataaaaaac actaattatg
     7081 aagaacatac atacgaagac aatctataca atgatataga aaaggtatat aataaagaag
     7141 aacgtatgtg ctatagaaat aaaaagcatt gcaagtattg tgaatttaat aagttatgta
     7201 atggcaaaga aatagattat agtattttgg aggcggaaat ttatgggact taggtttgta
     7261 ttaggtagaa gtggaagtgg aaaatccacc tatatactag atgaaattaa aaaagaagct
     7321 caaaaaaatg aaacgacatc aattatactt ttagttccag aacagtacac atttgaggct
     7381 gaaaatagag taagtaaact atttttagga aaagaaaaag ataagtatct aagagttagg
     7441 gtacttagct ttaagacact aagtaatata gttttttctc aagtaggagg attgacagat
     7501 gtaaatataa attctagtgg aaaagcaatg atggtatata gagctattga agatgtaagt
     7561 gaagaattaa atgtgttttc aaaatcaaaa tcgcagtctg gttttgtaag ttcaattaca
     7621 gatatgatat cagaaatgaa acaatacaat ataagccctg aaatgttaga aaatatatct
     7681 ggagaattgg ataatgaaac tttaagttta aaattaaaag atataagtaa aatatataat
     7741 tcttttgaag ggaaattgca tgaaaattat gtagatgctc aagatatgtt aacttcattg
     7801 gcaagtaaaa ttgaattaag cagttattta gatggagctt gtgtttatat agatgaattt
     7861 acaggtttta caccaaatca gtacaatgtc ataaagagta tattaaataa gtctaaaagt
     7921 gttaatatct ctttgactgt agatgatatc aactatattg ggtacagtaa atctgatatg
     7981 ttttctagaa cgaaatttac ttattcaaaa ttgactcaat tgtgcaacga agaaggcata
     8041 aaaatattgc cacaagtaaa tttaaacaca ggtgttataa aaagatttga aaaagttaag
     8101 gaattacagc acttagaaag attttataat gcttatccat ataaaatata ttcaaatcca
     8161 actgaaaata taaagataaa agagtttaac aatttatatt cagaggtgga agagatagcc
     8221 agagaaattg tacatttagt tagagataag aatgtcagat atagagatat aaccattgct
     8281 acaagagatt taaatagata tgatttttta gtacactcta tatttaatga atataatata
     8341 ccaaacttta tagataagaa gagagaggca aaaagtaatc caatagttat tttaattatt
     8401 tcagctttag aaatgaagaa tagacgatat gggtatgaaa ctatgtttag gtatttaaaa
     8461 agtggactta taggaattga taatgatgat ataaatttac tagaaaatta tgtgttggct
     8521 aatgggatta aaggtaaaaa atggtttgat gaaaaatggg attatagaat aacccaaagt
     8581 ctttcaggtc aagaaagtga atttgaactg gaattaaaag agaaaataaa tgaaataaaa
     8641 aatagagtat tagagcctat agtaatttta caagaaaaat taagaggaaa aaatagagta
     8701 aaagaaatct gtagatatat atatgaattt ttattagata taaatatgcc agaaactata
     8761 gaaagtttaa tagtaaattt taaggataaa ggggaacttg atgttgcaaa tcaatactca
     8821 caagtttggg acatagtagt ggacatatta gaccaaatgg tggaacttat gggtgatgaa
     8881 ataatttctt tagaaaaatt tataaaatta ataaccttag gttttgatga atatgaatta
     8941 ggactggttc ctcctagtat agaccaggta cttgtaagta gtgtggatag aatgaaaaat
     9001 ccagacacga aatatttata cctagttgga actactgatg gtgtgtttcc gctgattaca
     9061 aaggatagtg gaatattgag tgataatgat agggaaagtc ttggaaacaa aggaatcgaa
     9121 gttgatattg atagtaagac taggagtttt gaagaacagt ttcttgtata taaagctctt
     9181 acttcgacat ctaagaattt aaccataaca tatccaatat cagaccatga aggtaaaacg
     9241 cttagaccat caataattat atcaagactt aagaagatat ttccaaatat agaaaataaa
     9301 agttatttag tggaagaaaa taaaagcact gataaagata tacttaaaaa aataacggtt
     9361 aaatccccta catttaatga acttataaac gtaataaaaa attatgacag tgatggttac
     9421 aatacagaag agattaatag tatttggtta gatatatata ggtactattt aaaagatgaa
     9481 atctatagtt ctataacaaa aaaagttata aaaggtctta gttatactaa tcaagttcat
     9541 aagattgaag agaaaaaaat aaggtcattg tataaaagta attctcttag catatctagg
     9601 cttgaaaaat atgctgaatg tccatttgct tactttatac aatatggtct aaaagcaaaa
     9661 aagcgtaaag agtattcatt tacaccacca gatttaggaa cttttattca taatatttta
     9721 gataggtttt caaaagaact tttacaagat aatttaactt ggagagatat tgatgaaaaa
     9781 tatatagaat taaagatagg tataatagtt gatgaaataa ttttaaagat accaggatat
     9841 atattaaata gttctgagag gtataaatac cttgcatata gattaaagaa tatgttgaca
     9901 actgctataa ctataattag tcagcaaata aaacaaggtt catttgaacc aatagattat
     9961 gaagttaaat ttggcaacaa tggaaaatat ccacctataa aaatggtgtt agaaaatgga
    10021 caagaagtaa gtttaattgg acaaatagat agagttgatg aatttgaaga aggagaaaat
    10081 aaatatataa gaataataga ttataagtca ggcaataagt ccataagtct aacagaaata
    10141 tattatggtc tacaattaca attacttgtg tatttagatg caatccttga aagtgcaaag
    10201 gatgaagata taaatataaa tccagctgca atactatatt gtagaataaa taatccaata
    10261 gcaaaattta atgaagataa agatgatgaa gaaatacaag aagcaatatt aaaagaactt
    10321 agaatgaagg ggcttgtagt aaaagactct cacatagtta aagagatgga taaatcatta
    10381 atagatggag aaaggaaaaa ctctttagta ataccagtag gtcttactaa ggatggaaat
    10441 gtaggtaaaa gtacttcagc aataagctat gaggatttca aactgcttag aaaatatgta
    10501 aggcatgcta taaaggattt atgtgaagaa atgttaagtg gagaaatacg tatagctcca
    10561 tataaacata aagatggaac atcatgtgac ttttgtgatt actctgctat atgtcagttt
    10621 gattcaacta taaaagataa caagtataaa aatttaaata ataagagtaa tgaagagata
    10681 ataaaaatga tgaaaggaga tgttaattag tgagttctcc taaatggaca aaagagcaat
    10741 tagaagttat agaaagtaga gaatgcaatt tattggttgc tgctgctgct ggttcaggaa
    10801 aaacagccgt tctagttgag cgtataattc aaatgataac aagtagagaa aatccaattg
    10861 atatagataa gttattagtt gttacattta ctaatgctgc tgcgtcagaa atgagagaga
    10921 ggatagggga tgctatagga aaagccttag atgaaaaccc agaaaataaa catttacaaa
    10981 accaacttgt tttattaaat aaatctagta ttacaacaat acactcattc tgtttggatg
    11041 ttataaaatc aaactttcat agaataaact tagaccctaa tttcagaata ggagaccaaa
    11101 ctgaatgtgc tattttgaaa caagaagcaa tagaggaagt atttgaagat ttatatgaag
    11161 aaagagatga ggggttttta aatttagttg aaagttatgc agaaagagga ggagataaag
    11221 aagtacaaga tattatcttg ggaatatatt catttgcaat ggcttctcct gaacctaaaa
    11281 aatggttgat tgactcagca gaacgattta atattgatga aaattttgat ttttcacagt
    11341 ctatttgggc tagagctata ttagacactg taaaaataga gattaatggt ctttgtctaa
    11401 atatggaaag ggcattaaaa gaagtggaat caatagaaga attagaaact tttgcagaaa
    11461 agttatccgt tgaatataaa aaaattgctg acatttcaca agcatgtaat aaatcttggg
    11521 atgaggcata taaaaagatg gcaagtatgt cctttgaaaa ttatgtaaaa ggagtaaaaa
    11581 gaatttcaaa agatgctcct tcgtatataa aagaatcaaa agaaaaggca aaaactataa
    11641 gagataagac taagaagtct ttagaaagta tagtaagtgc tacattcaat aaagataatg
    11701 attctattag agaagaaata aaatatcttt ataacatagt taaaccaata tctagtgttg
    11761 tactgagatt tgaagaggaa tatagcaata agaaaagaga aaaaggaata atagatttta
    11821 acgatataga gcattttgca ttaaatatac ttacagatgt agatgaaaaa ggtaatattg
    11881 taccttctga tatagcagtt ggatgtagaa acaagtttta tgaaatattt atagatgaat
    11941 atcaggatag taatctagtg caggaagtac tgcttaaagc tgttgcaaat acagaaactc
    12001 caaatagatt tatggttggt gatgtaaaac agagtatata tagatttaga caagcaaagc
    12061 cagaattatt tttgcaaaaa tataataact ataatgataa aaagggaagc tcccatagaa
    12121 aaataatgct ttacaaaaac tttagaagta gagaagaagt tgtagatgct gtaaattata
    12181 tatttgaaaa tataatgaat gagaatatag gagaaattga atatacagaa aaagaaagac
    12241 taaatttagg tgcaaatttt aatgtggata cagacgagaa atctattatt ggtggagcaa
    12301 cggagataca cctaatacaa aaagataata aacttgatga tgatattata aatgataaag
    12361 acgatagaat taataacaaa gaaaatgaaa tagaggaaga agaaaaatta gataatatac
    12421 aacttgaggc tagaatggtt ggaaacatta tcaaagattt aatgaaagtc aatgaagatg
    12481 gaaaaattca aaaggtctat gataagggaa tagatggata tagacctgtg gaatttagag
    12541 atatagttat tcttttaaga gctacatctg catgggctcc agtgtttgca gatgaattga
    12601 tgaatatgga tataccaacg tatgctgatg ttggagtggg gtattttgat acgattgaaa
    12661 taaaaacaat attgtcatta cttcaaataa tagataatcc aatgcaagat atacctctta
    12721 tatctgtatt aaaatcacct atatttggat ttacaccaga ggatttgatt gatattagag
    12781 ttcagagtaa agataaaatt ttttatgagg ttttaaaaag tacagcagag tatgatggat
    12841 ttacagattc tcaaaatgaa aatgagtctg agtttatacc aagtgaagag tgcataaaca
    12901 aaagtaaaga ttttttaatt aagctaaagg aatttaaaga aaagtcaatg tacatgagta
    12961 cagatgaatt tatatggtac ttgtacacaa ggactggata ctatgcctat gttggagcat
    13021 tgccaggtgg aagtcaaaga caagctaatt taaaagtatt gtttgagaga gcaaaacaat
    13081 ttgaagaaac cagtcttaaa ggaatattca actttgtaaa ttttatagag aaattaaaaa
    13141 aatctagttc tgatatggga agtgcaaaaa cacttggtga gaatgcaaat gttgttagaa
    13201 taatgagtat tcataaaagt aaaggtctag aatttccagt agtcatatgc tctgcaatgg
    13261 gtaaaaattt caatactcaa gattttaaaa agagtatttt atatcatcat aatttagggt
    13321 atggacctca atttgtagac tatgagagaa ggatttcttt tccaagtata gccaaagaag
    13381 cattaaaaag caaaataaat atagaaaatt tatcagaaga gatgagagtt ttatatgttg
    13441 catttacaag agctaaagaa aaattgatta taactggttc tactagaaat atacaagata
    13501 gtataaagag atggtcaaat ggaattgaaa gtttagatac aatatcacaa tacgagatat
    13561 taaaaggtaa aaatttttta gattggataa tgccttgtgt attaagacat agggatttat
    13621 ctaatctact agaagaagta ggattagacg cagtttttaa tgttgaacat aattcaaaat
    13681 ggtatggaaa gttgtggaat aagaatgata ttttagtgga aaagaagagt gatgaagaaa
    13741 aagaaagtat tgaagaaatt cttgaaaaaa tagatgtaaa taatcctgac agtgattatt
    13801 acggtgaaat agaagaaaaa ttaaactata tatatccata tgaatttagt acaagaaaac
    13861 ctgctactat atctgttaca gaaataaaaa agatacaaaa taattatgaa gaagagttaa
    13921 taaatactat atttgaacaa aaagtaatat taaaaaagcc tctatttatt caaaatgaag
    13981 aagaaagaga aaaaataagt ggaacagaaa gaggtacaat agtccattta gttatggagg
    14041 ttttagactt aaaaaatgta agtagtgtaa atgatataaa atcacaaata agaggttttg
    14101 tttcaaaagg tataataaca gaaaaacaag ctagtatagt aaatccatat aaaatatata
    14161 aattttttgc ctctaacata ggaaaaagaa tgttgaatgc tgaaattata aatagagaaa
    14221 aatcaattta tgcacaagta aatatgaaag acatatatat ttatgaaaag cttataaaca
    14281 atgacgataa aaaactttat gataatgaaa gtgtcatgtt aagaggtata gttgatgctt
    14341 attttgaaga agataatcaa atagttttgg tggattataa aacagatttt gtaaatgagg
    14401 aaaatataaa tcaaataata gagaaatata aaaaacagtt ggatttatat gcagatatta
    14461 ttgaaacttt aactggtaag tcagtaaaag agaaatgtat atatttattt ggagttgatg
    14521 aagctgtttg ttattagttt ttatatataa attaaaccta aaagatagag gaaggggaaa
    14581 aaatgagatt tattcataca tcagattggc atctagggaa gtccctagaa ggccattcta
    14641 gaattgagga acaagcaaag ttttgtgagg aatttataaa aatagtagaa tcaaatgaaa
    14701 tagatatggt tataattgct ggagatgtat atgatacttc taatccccca gcacaagcag
    14761 aaaaactatt ttatcagact gtatcaagac ttgccaataa tggtcagagg tgtgtattaa
    14821 taatttcagg aaatcatgat aatccagaga gactctctgc aataacacct ctagcacatg
    14881 aacaggggat acttatatat ggttatccat taagtgctac gatagaagct aagtataaag
    14941 ggtttgaaat aacatatgca actcagggat gtacaaaact gaatataaat ggagaaaata
    15001 ttgtggttgc tacattacct tatccaagtg aaaaaagatt aaatgaagtt ttttcaagtg
    15061 aagatgagtt tgagaaacaa aaaaattatt ctgaaaaggt aggagatata tttagaagtt
    15121 tagaagaaaa ttttagaagt gatactataa atatagccgt atcacatata tttgtaatag
    15181 gtggagagag tactgaatca gaaagaccta tacagttagg tggaagtttt ttagttgaaa
    15241 gaaaagattt gccagaaaag gctcaatata ctgcactagg tcatttacat aaacaacaaa
    15301 aagcttccga acgtttaaat gcatactatt ctggttctcc acttcaatat agcaaggatg
    15361 agagagcgta taccaaaggg gcatacatag tggatataaa agctggtgaa aaaccaataa
    15421 ttgaagatgt atattttaat aattacaaac ctatagaagt atttaaatgt aatggaatag
    15481 aagaagcact agatatatgt gagcaaaatc aagatagaga gatttggtca tattttgaaa
    15541 taaatactga tgagataata tcacagaatg aaattaaaaa aatgaaggaa ctattaaaag
    15601 acataataga aataaaacca ataataacat cttgttatga acaagaaagt gtagatataa
    15661 aagaaaagtc tatggcagag ttgtttagag agttctattc ttttagtaaa ggtgtagagc
    15721 caaaaggaga acttatggat ttatttctag atataataag tgaagaaggt gaatcagcag
    15781 atgagaccaa ttagattaga acttacaggg cttaatagct atattgataa acaagttata
    15841 gactttgaaa aattaattga aagaggtttg tttgggatat ttggaactac tggaagtgga
    15901 aaatctacaa tcttagatgc aataactatt gctatgtatg gaaatatatc cagaaataca
    15961 aaagaatata taaacagtgt gtgcgataag gcaattatat catacgagtt tgagatagga
    16021 agcaaaaaca ctagaagaag atacattgta gatagaacca ttgctagaag caaaacagga
    16081 acaaagacct ctaatgctag attaatagaa gtattgaatg acaataccca aaatgtttta
    16141 gctgataaag ttgtggaagt aaatgaaaaa gtagcacagg ttgtaggatt aacagcaaat
    16201 gattttacta ggtcagtagt tttaccacaa gacaaattta atgagtttct aagattatca
    16261 cgtgcagata gaagagacat gttagagaga atttttaatc ttgagaaata tggaagaagt
    16321 cttggagaaa aagtgaaaaa gagaaaaaat atacagttac aaaatttaaa agatttgaaa
    16381 tcaaaattaa gtcaatatga tggaattaca gaggaagtat acaacaatat aaaccaagaa
    16441 ttaatagagt taaaaaattt ggaaaaagat aaggataatg ctttagacct agctcaaaaa
    16501 tcttatgaag aaagtaaaac agtatatgaa gaacaattaa agttagaaaa aaatgaactt
    16561 agaaaaaatg agcttgattt aaaaaatagt gagataaaag aaaagattgc tcttgtagaa
    16621 aatgatagta acgctaaaaa ggtagaccca tacataagtt cagttcaaaa tctagaaaaa
    16681 aagatagatg aagatagctt cgcagtaagt aatttagaaa agaaattagc aatattaaat
    16741 caggagcttg aagttacaaa aaatagatat gaaaaaataa acaaaattaa gaatgaagaa
    16801 gtccctaaat tgagtgaaga aaaaataaga ttgcaacaag caataaaatt agaagaagag
    16861 ttagttttat tagatagaga gttaaaagag ctaaaagaaa gtggaattaa tttaaataag
    16921 actaaagttg aattagaaaa agtaaagcag gtgtcagagt ccagaaaaga tgcagttact
    16981 aaatctataa aagaagttga aggaaagatt gataaagtaa atataagtgc agaattaaaa
    17041 caaaaaatat ttctagcata tgaatacgag aaagattaca acaacgtatt ggaagaaaaa
    17101 aatcaaaagc taaataaact agaagaaata ctcaaagaga cagaaaatat aaatttaaaa
    17161 gttagatata ttgataaaga taagaatgat gtaaatcgta atttagaaaa tttatcactt
    17221 cacttggatg ttcttcttaa aaaatgtcct ggtaagagtg ctgatttact tttaaaatct
    17281 gaatatgtta ctgaattaag aaataaagca aataatacta aagaaaatga aattaagaaa
    17341 agcagtatac aagatgaatt aaagataata cttgaaagta agtttaatac tgaaagggaa
    17401 gtaaatttac taaatgaaaa acttgaaaat aatagaaaga acagagatga cttagaaaaa
    17461 gaattagaag aactaaagta tttaaattta gcctctgaac ttagacgtga attaaaagaa
    17521 aatatgcctt gtccagtttg tgggtcaaaa catcatgaaa atcatgatat aactaagtat
    17581 gatgaaaata tatcttttgt caaagaaaaa ctggagaaat tagaaaaaga aaaaataagt
    17641 ataagaaaca atatagagga attaaatgct aaagtaagtg ggtatttatc tatagaaaaa
    17701 atgaagacta aagaacttga agatgtaaaa ggaaaactag gggggattcc atctagtcaa
    17761 ttattaaaaa aacttgatga agaacaaaga aaacttgcat tgttaaaatc taatattcag
    17821 gagtgggaga aagaaaaaga gagtactgaa aataaagtaa cattagctaa agaagaaaaa
    17881 aataaaattg aaaaagaaga attaaaaata agagagagtc tgaataatta taagaaatta
    17941 acaagagatt taaacataga aatagaaggt ttagaaaata aatgtaaaaa acttaaacag
    18001 gaatacctag gattaaagac gattactaaa gtctctaatc tattgtcaaa agtaaaagaa
    18061 ataagagaga atgaaaaaga attagaatta ctaagtactg ggcattcaaa tttattaaaa
    18121 aatagagact cactagatat taaaataaga gaaaatgaga gtaaactaca tgaaatagag
    18181 atagaattaa taaaggctag agaattatat gtagaaaaga aattgagtag agataataag
    18241 tataaagaag ttattagcat aacgaaaggt gatttagcta aaaatctttt acataatgtt
    18301 gaagaaaata tatgtaagat attagaacaa gaagaatcaa gtaagaaaaa attggaagag
    18361 caaagattag aatatgaaaa aaacattgct gaaaagcaca atatagatgg tagacttaaa
    18421 actgctaagg aacaatataa agaccaaaaa tcaacactaa ataaactatt agcagaaaat
    18481 aaatttgaaa gtatatatgc agtaaaaaga gctttattag atactgatgc agtaaaaaaa
    18541 ttgattgaag aaatactaga gcatgaagaa gaacaaaagt tattatcatt taaaataaaa
    18601 tcatcaaaag aaaaattaaa tggaagaagc ataaagaagg agtactttga acagttaaaa
    18661 gatgaaattt ataatttgaa agtagaaata ggaaatatat ccaaagatat tggagctaat
    18721 caaaatcagt taataacatt aaaagactct ttagataaga taaatgattt taataaacaa
    18781 cttaaggtag ttgaacacaa tgtggactta ttggaagaat tagataaatg tgtacaagga
    18841 aataaatttg tagaatatgt ttctacaaac caattaaaat atatagcgtt ggaagcatca
    18901 aaacgattgg atggaataac aaaaggaaga tatgcacttg aaattgactc aacactaaac
    18961 tttgtaatga gagataactt taatggagga gaaagaagaa gcattgatac tctatctggt
    19021 ggagagacat tcttgacatc tctatcatta gcacttgctc tttcatctca aatacaatta
    19081 aagggaagtg caccattaga atttttcttt ttagatgaag gttttggttc attagatagt
    19141 gaacttttag aaattgttat agaatcattg gaaagattgc atagtaataa tctaagtgtg
    19201 ggtataataa gtcatgtcga ggaacttaaa aatagagttc ctgttaagct attagtttca
    19261 tctagtgagg ctggaatagg ttcaaaagta aaaattgaat atagttagaa aatatatgtt
    19321 aattgaatat attatctaat atttccaaat aatatgtaat tgtgatataa taaaaactaa
    19381 tattaaagtt atttataatt aatattaata taaaaacatg taagtaggag tgtggtaagt
    19441 gttggaatcg cccaataatt tgattcagat tattttttta atagtattac ttatagggtc
    19501 tgcatttttc tcagcatctg aaacggcttt aatgtcgttg agtaaaatca gaatcagata
    19561 tatgcaagat gaaggagtta aaggagctaa gttagtaagt tcattaatag aaaatccaaa
    19621 taagctttta agttctatat tggttggaaa taacgtcgta aatatagcag caacatccat
    19681 atcaacatct ttgtttatag gattaatggg agaaaaagga gtggcacttg ctacagctgt
    19741 tatgacagta ttagtattaa tatttggtga gattactcca aaaacaatag cagcaaataa
    19801 ttcagaaaag gtttctctat tagtatctaa acctataaaa gcaattatat ttatattaag
    19861 accaatagta tggatattta atataattac aaatattata tttaaattat ttggtataac
    19921 aaataaagga gctaaatcat tcataactga agaagagcta aaaactatgg taaatgtaag
    19981 tcatgaagaa ggcgtccttg aaatggaaga gagagaaatc ataaataatg tttttgaatt
    20041 tggagatatg caagctaaaa atgcaatggt acaaagaata gatatggtag ctatagatat
    20101 ggaagatagt tatgatgaga taatacaggt atttaaaact gaaaaattaa gcagaatgcc
    20161 tgtgtatgaa gaaactatag atgatattgt aggaatactt aatataaaag atattatatt
    20221 tttatctgat gaagaaatag agtcatttga tattaaaaat tatatgagag aacctttttt
    20281 cacgtatgag ttcaaaaaga taacacaact tcttgaagag atgaaacttg aaaaaagtca
    20341 gatggctata gttgtagatg agtatggtgg aacatctgga cttttaacta tagaagattt
    20401 agtagaagtc attgttggag atatagagga tgaatatgat gaagaagagg atgaaataca
    20461 agttataaaa gaagatgagt atattgtaga tggaagtact aaaattggtg atgttaatga
    20521 acttattggt gtaaacttgg aatctgagga gtttgattcc ataggaggtt ttataattgg
    20581 acacctaagc agattgccag aagaaaatga agtaattgag gttgataata taagattttg
    20641 tatagaaagt atagaaaaaa atagaattaa aaaaataaga atatatacat aatgtaataa
    20701 tgttcttaaa tttat
//
LOCUS       DAMJZE010000057        20582 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJZE010000057 DAMJZE010000000
VERSION     DAMJZE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.057"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            84..1514
                     /locus_tag="PC541_001821"
     CDS             84..1514
                     /locus_tag="PC541_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839287.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            1714..2298
                     /locus_tag="PC541_001822"
     CDS             1714..2298
                     /locus_tag="PC541_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2839288.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2402..3181)
                     /locus_tag="PC541_001823"
     CDS             complement(2402..3181)
                     /locus_tag="PC541_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2839289.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3239..4495)
                     /locus_tag="PC541_001824"
     CDS             complement(3239..4495)
                     /locus_tag="PC541_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2839290.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4753..5652
                     /locus_tag="PC541_001825"
     CDS             4753..5652
                     /locus_tag="PC541_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2839291.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5676..7124
                     /gene="ddlR"
                     /locus_tag="PC541_001826"
     CDS             5676..7124
                     /gene="ddlR"
                     /locus_tag="PC541_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2839292.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            7183..7989
                     /locus_tag="PC541_001827"
     CDS             7183..7989
                     /locus_tag="PC541_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2839293.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            8015..9934
                     /locus_tag="PC541_001828"
     CDS             8015..9934
                     /locus_tag="PC541_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2839294.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(10176..10523)
                     /locus_tag="PC541_001829"
     CDS             complement(10176..10523)
                     /locus_tag="PC541_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2839295.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(10510..12438)
                     /locus_tag="PC541_001830"
     CDS             complement(10510..12438)
                     /locus_tag="PC541_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2839296.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(12850..15039)
                     /locus_tag="PC541_001831"
     CDS             complement(12850..15039)
                     /locus_tag="PC541_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2839297.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            15370..16101
                     /locus_tag="PC541_001832"
     CDS             15370..16101
                     /locus_tag="PC541_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2839298.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            16181..16816
                     /locus_tag="PC541_001833"
     CDS             16181..16816
                     /locus_tag="PC541_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2839299.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(17067..17660)
                     /locus_tag="PC541_001834"
     CDS             complement(17067..17660)
                     /locus_tag="PC541_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2839300.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(17731..18594)
                     /locus_tag="PC541_001835"
     CDS             complement(17731..18594)
                     /locus_tag="PC541_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2839301.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            18757..19926
                     /locus_tag="PC541_001836"
     CDS             18757..19926
                     /locus_tag="PC541_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839302.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            20027..20503
                     /locus_tag="PC541_001837"
     CDS             20027..20503
                     /locus_tag="PC541_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2839303.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         7572 a         3068 c         2639 g         7303 t
ORIGIN      
        1 tatataaata tcaaatttta agtatattaa tcaattaaac ctattttaat atatcattaa
       61 aaatttagga ggaaaatata aaaatgaatt tagatttgga attttatcaa aaaatattag
      121 aagcttccca tgatgaaata tgtgtttctg atgataaagg aataataata tattgtaata
      181 aagcatttga agaaaattat ggtctaaaaa aagaagatat tttgggaaaa aatgtttctt
      241 ttttagaaga ctctggatat tcaacaaaaa gccccatacc aattgtatta aaaacaaaga
      301 ctaaattttc actggaacaa gatactcaaa cagggaaaaa gcttatcata actgctaccc
      361 ctatatttga tgaaaatgga cacttagaat ttactgttga aaattgtaga gatataactg
      421 aacttaataa tataaaaaat aaacttgaag acacaaaaaa gcaagtaaaa aaatataaat
      481 ctgaagtaga aacactatac agaactgcac ttagaattga agatactgta attatggacg
      541 gaatagttat gagaccaatc ataaacacag taaatcatgt ttctaaaacg gatgtaagtg
      601 tacttttatt gggtgaatct ggaacaggaa aaagctcact tgctagatat atacaccaca
      661 acagcaatag atctaatggc ccatttataa caataaattg tgcaactata tctccacaac
      721 ttcttgaatc tgaacttttt ggatatactt caggtgcttt tacaggtgca agtactaaag
      781 gtaaagttgg gctcgtagaa ttagctaatg gaggaactct ttttttagat gaaataggag
      841 atataccaca aaatctacag gcaaaatttt tgcaattaat acaggataga acttttactc
      901 cagttgggag cttaaaaaat aaaaaggtcg atataagaat tatatctgct acaaatgttg
      961 atttagtttc aaaagtaaaa gaaaaaaagt ttagagaaga tttatactat agattaaatg
     1021 ttatagaaat aaaattgcct cctcttagag aaagaaggga taatctagtg gaaattataa
     1081 aatattattt taataggtac tcaagtgact tcaacctaaa taaaactatt tctaaggaag
     1141 ctatggatgc tattgcaaat tatagattcc caggcaatat aagagaatta caaaatataa
     1201 ttcaaaaaat cttgcttact tgtacagata atcatataac aatagatgac ttaccaaaca
     1261 ttcttacaaa aaatatacat attaccaata atggaaataa aacacatata tcccaaatta
     1321 ataaggttat tatatctgac tcaaaatcta ctaattataa aaataaaaat tttgacactt
     1381 taattaaaga gtatgaaaaa aatataattt tggatgctta tgaaaaattt ggtagttctt
     1441 ataaagtagc caaacacctt gaaatctcac agtctaaagc caataggtta attagaaagt
     1501 atactaatac ttaattttaa atttgataaa taaaaactta acactattta catattgtaa
     1561 atttggccat ttactgttat tatgaatatg gttgaaatta tttaaataaa tactgttgac
     1621 ttagtttcat atatttacca ttaaactttt atcataaata tcatatcata ataatatatt
     1681 taataagtct aataaactat acgaggtgta aatatgttaa tgaataccca ttttattatg
     1741 gcaaaatcaa tacttgataa tatagatgaa aataaaacat tctttataag cgaaaaaaat
     1801 tttatatatg gcaatataaa gcctgatgct ttttctaaat ataaattaaa aaaacattat
     1861 ttagatgagt catttaatat gattgtttct aaaataaact atctatgcag tcttaatata
     1921 gatagtcttt ctaaatactt ttcagtaagt agacttagtc aggaactagg agtcatatgt
     1981 cattttttat gtgatttttt ctgtgtagct cacagtgaac gttgggaatt taagcatagc
     2041 ttaaataaac atgttactta tgaaagagaa cttggagcat ttgcaaaaga tgttgattta
     2101 tctaaaatta aatgtggtac tataaatagt agttttgaga agttttttac taaactttat
     2161 agtgattaca aaaagaaaac tgattatatg aatgatttaa aattttcagc ttatacttgt
     2221 aatagtgtca taaattatat acttgactct attttatcta atacaataaa atcatatcat
     2281 atagcaaatt gcggttaact taagaatttt taaacttata taaacttata attgaatata
     2341 attaaaatga ctgctctata gaatataatt ttatacttct ctaggacagt cattttttat
     2401 actaactatt tatctccatt ataacatcta tggcactttt tacactatct actacataat
     2461 ttgcactttg ctttacatct tcccttgcct tagacattgc aaaactatat ttaaatcctt
     2521 ctaacatgca aatatcattt ggagaatccc caatagtagc aacttcatct ggacttacat
     2581 ttaacgtttt tattacatgc tctatacttc ctctttttga cacactttta ggaactatat
     2641 ttaaagtata tgttccagaa aaatatattt ctaagacttc actaaactca ctttttagat
     2701 aaagttctat tctggataac tcttccaaat tttcagaaca tacgcctata tttcctataa
     2761 gattatcaaa actacttctt ttttcaaaat ctgggtcaac tataaagtca ctagaataat
     2821 gtctaaaagc agtggtatca ctacccacaa ttacatcacc cttatattta aaataaagtt
     2881 gtgcatcact tgactcaaat ctatcaatga ctttttttac taaattatca tctatcgttc
     2941 ttttaaaaat tacattataa tctttatcat atataaatga accattttca catacatagt
     3001 atccattcat atctaaacta tctttgataa tattatgtgc ttgtttaaaa attctacctg
     3061 tagcaacatt aaactgtact cccttttttt ctatttcttc aattgctact atatcttctt
     3121 ttgttatggt tccattttca tatagagttc catctaaatc acaaaatata tgttttatca
     3181 taaattaaaa gcctccaaaa acaattttat attttactaa tactttaatc aaaataactt
     3241 aaagcttttc taattgtatt ctcaattcat taagcaaatc tctagtagct cttaatctta
     3301 tctttcttct atccccatta aatatatttt tctttactat tgtttttcca tttatatata
     3361 ttgcagtata aaccagtccc acaggcttct ctttggttcc tccaccaggt ccagctactc
     3421 cagttgtaga tatacctata ttagctttaa gtcctttagc aatgccctca gccatttcta
     3481 tagctgtttg ctcacttact gctccaaact cttccaaagt ttctcttttt actcctaatc
     3541 ttttcatttt agaatcatta gaatatgtta cacaaccttc aagaaataca gaggatatac
     3601 ctggatagtt tataagagat gaagaaacca aacctccagt acaagattca gccactgcta
     3661 ttgttagatt tttttctaca agtagcttag aaacagcatc ttctaaagca gtctcccctt
     3721 cagatatgga aatatctcct tcagtatata catatttacc aactctactt tttatttttt
     3781 cttctactgg ttttattaat ttaaaagctt ctctttcatt ttcagccttt gcagttattc
     3841 ttattgtaac ttctaattct tttgcatata aagcaactgt tggattagtc tgctcattaa
     3901 ttatatcgag aatttctaat tctagatttg actcacctat tccatataat cttaaagtct
     3961 tagatactag catttcattt gtgaattgtt gtaagtaagg tttgacactc tcattaaaca
     4021 tagctttcat ttctcttgga ggtccaggca atactattat aaatttacca tcttttttta
     4081 aaattgcccc tggagctgta ccattattat tttttagtac tatagcatct tttggaaagt
     4141 acacttgttt catattattt tcggccattt ttttccctgt tttaacaaag aaagattcca
     4201 aaacctctag tgaaggttta tgtagttcta atttctgacc aaagtattct gcagcagttt
     4261 cttttgtcat atcatcacca gttggcccaa gaccacctgt agtaattaca aaatcacttc
     4321 tctttaagct ttcatcaaaa cattctaaaa gtctttgttt gttatctcca acagtactct
     4381 gatggtatac ttctattccc aatgatgcaa gttctttagc taaaaattga gaatttgtat
     4441 ttactatatc tcccaataat atttctgttc ctacacttat tatttcagct ttcaaaattt
     4501 atcactcctt gatttatagc cattaagact agtcttaagt tcataacaat aattttagca
     4561 aaaataataa cataaataaa gttaaaaaat catttaaaca tatctttaaa ctagattatt
     4621 attctaaaac cacacataat ataaacttcg tatatagtgg tactttcatc ctcaactgtt
     4681 aattcatata attaagtatg taaataaaaa gtttacttta aaataataaa aattaaaata
     4741 ggagtgtata atatgaaaat agcagttata atgggtggaa tatcatcaga gagagaagtt
     4801 tctttaaatt caggaaaaga gatatataat aaccttgata aaaataaata tgaagttgta
     4861 aaggtaataa tagatgataa aaaagatata tttacaaaga taccagaaga tatagatttt
     4921 gcaatacttg ctttacatgg aaaatttggt gaagatggat gtatacaatc aatacttgag
     4981 actatggata taccatactc tggatgtgga cctctttgca gtgggatgtg tatggataaa
     5041 aatataacta aaaaaatgct tagagattca aacctaccta ctgctccatg ggttttagta
     5101 aaatcagttg atgaaataga ctatgatgaa atagataata taggttatcc agtatttata
     5161 aaaccaaact caggaggttc aagtgtagcc acattcttta ttcattctaa ggatgaagtt
     5221 gaagaagcag ttagaaaagg tctggaagtt gatgaatttg taatgattga aaaatatata
     5281 ccaggtggag aatatacttc attcatatta aatggagaag tgttccctac aatatctata
     5341 aaatctgatt caggattctt tgattatgaa gctaaatact cagttgaaaa aggtgcaaag
     5401 gaagaagttg tttacttaga tgaagaactt caaaaaagag taaacgaaat ttctgagact
     5461 tgttggaaaa tttttaattg caaagcatat gtaagagttg atatgattat aagtgaaggt
     5521 attccatatg ttcttgaact aaatactctt cctggaatga ctcaaactag tttaattcct
     5581 agaagtgctg ctgctagagg tattaagtac agtgaactat tagataaact tatagaatac
     5641 tctttaaatt agatgaaata atttgaggtg gtaatttgat attttcaaat ctaaaattaa
     5701 atgataatga accaatttat atacaactaa aaaattatat aagtgatatg atatcaaaag
     5761 gactaattcc agacaatagt aagctccctt ctacaaggga gcttagtcag cttttacaag
     5821 ttagccgaaa ttctgtagtt ttaacttatg aagaattaaa atcagaagga ttaatctact
     5881 ctatttcagg aaaaggtaca tttgttaaat caaagaataa gtcttcaaat actacatggt
     5941 cattaaactg ggattgttta gagaatacat actctaaaaa agccaatgaa ttggacatta
     6001 taaaaagtga aattccttgg agctctgacc tgatttcctt taaaagtatt tctccagatg
     6061 gagatttatt cgacatggaa gaattaaaaa aatccttttt aaatagaatt tcactagaag
     6121 gacataaatt actcaactat ggatatgcac aaggttataa gccacttatt gactatttac
     6181 tagaatatat gacaaacaaa ggtgctgata taagtaataa agatatacta ataacaaatg
     6241 gattcacaga aggctttgat attattatgt catcttttac ccaagaaaaa gactacataa
     6301 tatgtgaaaa tcctactcat aatactgcta taaaaattat gaaatctcat aacctcaata
     6361 tcttaggtgt agatataaat gaagatggtt tggacttcaa tatgctagaa gacaatttag
     6421 ttaagtataa aaataaaata aagtttgcat acattacacc ctcttatcac aatcctacag
     6481 gaattgtaat gactcctgaa aataggtaca aattctataa tcttatgaaa aaatacaata
     6541 tagcaattat agaagatgga tttaatgaag aattactcta caatagttct catatatttc
     6601 ctatctgttc cctagataat atgaataatg gggtcgtata tattggtagt ttttcaaaaa
     6661 ttttatttcc tggaatgaga attggatgga tatttgcaga taaaaaagta atcaataagc
     6721 tagaaagtgt aaaaaggtgc agaaacatac atgtatcctt tttagaccaa ggaattttat
     6781 ttgattattt aagtaatgga ggttttgaaa aatacattaa aaaaatacgt aaattctatg
     6841 gagacaaatt taactttgca tataaatgta taaaaaaata tataaaaaca gaatatatct
     6901 tgggtgatgg tggtttgcat atatttataa aactaaaaaa tatagatact agagttttat
     6961 tagaaaaatg ttatgaaaaa aatgttatat ttatgcctgg agacttattt tttacagata
     7021 actctggttt tgatacttta aggctcggat tttctaggtt gtcttttgaa gacattgaaa
     7081 ttggaataaa aataattgga gaaactataa atgaaatgtt ctaggttaac taaatattgt
     7141 tagcctagtt tatttttatg aggtataata tatatagttt atatgaatct aatactgggg
     7201 gataatatga gtaaaaagtc aaaacgaaga aaaattaata aattaaaatt aacgattgta
     7261 ataactgtat tatttcttgc tttattatct atatatgaga tactgttctc taatcccaac
     7321 aaaaaaggta atataaagag tcaagaaact atgcaaaata aaaataatac aacagatgat
     7381 tctaaaaata atcaaagctc taccaaaaag caggaagata ataatatcaa acctactttt
     7441 tcatattctt caataccaga taatataaaa aataaaatga ttggtaaatc tatgcctact
     7501 gatgaaccta taagctttga tagtctatct tatttaaaac ttacatatta tggatttgat
     7561 gaaaaaactc atcaaggaga aatgatagta aacagtgaac tagctccaga agtagtggat
     7621 atttttaagg aattatacga aaaaaaatat ccaattgaaa aaattaagtt aatagatgac
     7681 tatgatgctg tagatgaaaa atctatgtct gataataata cctcatcatt ttgttataga
     7741 actatagctg gtacaaatgt agtttcaaat catggtaaag gacgtgctat agatataaac
     7801 ccattacaga atcctcaagt ttctggaaat gatgttacac ctaaagtaag tacagtttat
     7861 gctgataggt ctagcacaaa atttggaatg ataaaaaaag gtgatgattg ttataatgct
     7921 tttgtaagca gaggttggag ttggggtggc tattggaaaa acccagatta tcaacatttt
     7981 gaaaaataat agattagaaa taaaggtgaa ttatatgaat ttaatgacat tggaaaatat
     8041 aagtaaaagc tattctgaaa aaaaattact agaaaatata tctcttggaa taaatgaagg
     8101 ggaaaaaata ggatttattg gtgtaaatgg tactggtaaa tctaccttac tcaaaattat
     8161 tgcaggagtt gaagaaagtg agactggtaa tataacaaaa actaatggta ttaggataga
     8221 atatctacct caaaatccaa cttacaatga aaattcaact gtacttgaac aagtatttaa
     8281 tggaacttct gaagaattaa agctacttgg tgaatatcaa gaaatcctag aaaaacttaa
     8341 ttcaaacttt acagaggaat taaataaaaa attattatca gtacacgaaa aaatagatgc
     8401 cttaaatctt tgggatttag aaagtgaagc taagtcagta cttacaaagc ttggtataaa
     8461 tgactttaat caaaaaatag cagaactttc tggtgggcaa agaaagagag tctctctggc
     8521 atcagccctt attacacctt gtgagttttt gatactagat gagcctacaa accatctaga
     8581 caatgatact atagattggc ttgaggaata tcttaattcc agaaaaggtt cattgcttat
     8641 gataactcat gatagatact ttttagaccg tgttacaaat agaattattg aactggacaa
     8701 aggtagatta ttcagttatg atgggaatta ttcaatattt ttggaaaaga aaatggaaag
     8761 actttcgcta gaagcaagta ttgaagataa aagacaaaat ttaataagaa cagagcttgc
     8821 gtgggtaaga cgaggagctc aggctcgtag cactaaacaa aaagctcgtt tacaaagatt
     8881 tgatgaactt ataaatagag attcttttaa acctgatgaa aatctcgata tatctgttgc
     8941 atcaagtaga cttggaaata aaataataga aatacacaat atatctaaat catttgaaga
     9001 aaatactgtt atagataatt tagaatacac tttaactcgt acagatagaa ttggtataat
     9061 aggcaaaaat ggtatgggta aatctaccct tataaatata cttaatggag aaatagagcc
     9121 tgatagtgga tatatttcaa ttggagaaac agttaaaata ggttgttttt ctcaagacga
     9181 ctctcatatg aatattgata tgagggctat agactatgta aaagaagcta gtgactatat
     9241 agaaacttca gatggtacta ggattacagc ttctcaaatg tgtgaaagat tcctttttaa
     9301 tggtactatg caatatactt tgataggtaa attatctggt ggtgaaagaa gaagactaca
     9361 tcttcttaaa atccttatga cagctcctaa tgtgcttctt ttagatgaac ctacaaatga
     9421 tttagatatt gaaacgctta aaatattaga agaataccta gacgaattta aaggtgtagt
     9481 agttacagta tcacatgata gatatttctt agatagaatt tgtaataaaa tatttgcata
     9541 tgaaggtaat ggaaaaattc atatctatac aggtaattac agtgattatc ttatttatag
     9601 agagattcaa ggaattgaat ttgaagaatc taaaagggaa tcagttaaaa aagatgactc
     9661 tactacccca aaaaaagaaa aacctaaaaa tgataaaaag cttaagttct catataatga
     9721 acaaagggag tttgaaaata ttgactctga tatagaaaaa ctcgaatcta aaattgcaac
     9781 acttgatgaa agtactgcaa aattttcaac tgattttact aaactccaag aaattttaga
     9841 tgagaaatct aaactagaaa aagaacttga atacaaatat gaaagatggg aatatttaaa
     9901 taatctagct gatgaaatag ctaataataa gtaatattaa aataatctta agtttaataa
     9961 gttttgcttt aaatctgtaa tttataaata ttttaaagga agtgtctcaa aatgaacttt
    10021 ttagttttat gagacactct tttttatgag aaatcaaata tattttcatc atttcaacaa
    10081 tacaaaagag gcttatctct attttgagac agcctctttc ataattacct tctaatttat
    10141 atgctctaat atatctataa taattcttat tatatctata tatccagtct ttcaatatag
    10201 tgacaattaa cttcaatatg atttaaaaat actaacaacc tttcataaag tggatgagat
    10261 tcttctccat atttagcttc taggttcttg cctatttctc taacagtcct ttgaccatca
    10321 atttgtaaaa aaacaaaact ggaatattca tctagttcta tttttttgta tttaggtatt
    10381 ctagctttta actttctaaa aaacttttga attttatggt cctgtttttc aagtatagta
    10441 acaacacgat tttcagctac ttcatattca agtccatcag taattttaaa tacaatatct
    10501 aaaacatcat tactcttgct cattattttt tactttagat aaaacaatag gtaatgctgt
    10561 aagcacaact aaaactacta ataaagcaat tgccattgaa tttgttgcag caaatccact
    10621 tggtaccttt ggagttacaa ctccagttac ttgtaatatt atacctatta aaccaatgat
    10681 agaaccacca gcaacaagac cagaagataa acttatacca ttagaaactt taacttcttt
    10741 ttctttttca gttttagata ctaattcaac aaataaacga attaatgctc caactaatat
    10801 tatagaagta gttgcaattg gtaaatagaa tcctatagct atagtcatta ttggtaaatt
    10861 taatgcatat agaactattg ccataaatac tccaacaata atcataaccc aaggtaaact
    10921 acctgacata ataccagaag ttaatgttga cattaagttt gcttgtggta atgcaaattg
    10981 tacattatca ccagttgctc taagttggtc agaaagtaat aaaattactc ctacaacaac
    11041 tataacacca actatacttg caattgtaaa gtaacgttgc atttcgtttt tgctaccacc
    11101 aataatataa gttacttttt gagattgagt ataacctcca gctatagcaa tagcaacaac
    11161 tataaatgaa ccaaataaaa gtaatgattt attagcttct aaatcagtcc atcccattat
    11221 aacaaacact aatgttacaa taactagaga agctatagtc ataccagaaa ctggaagatt
    11281 tgatgttccg attgttcctg ttaaacgacc agctactatg acaaataata aagataataa
    11341 aagagatatg atagctccaa taattgccat aacaatgtta ccagaaatta agaatgctgc
    11401 taaaaatcca ataactactc cacctaataa aagtatcatt tgtatagatg aaccttcttc
    11461 tccttctcca gcacttgatt tagctttaag tgtttctttt atagaagcga ttatagttgg
    11521 tataagtttt attgcaccaa taatacctcc acaaagcatc ataccagcac ctatatattt
    11581 tacataacta ctagatattg ctccaacatc catttggtta agaggcattg caggattatt
    11641 ccataccata gctccatctt tagccatatc tgtaaagtat cctattaaag gtgctatacc
    11701 aaagtttgat aaaatacttc ctgcaaacat tgttaaagaa acttcaagcc ctactataaa
    11761 tccaattcct aaaagtagag gatttacttc aatatcaaat ttccatttgt agaatttgct
    11821 tcctacaaga ctcattacat tgtttgctac atttaagaat gaaccagtta atacagtaat
    11881 aaatccactt attacaaatc caattcccat gtacttaatt gaatctccac cagcctctga
    11941 agcaacaagt gtctcagata tagccattga ttctgggtac ataagtttac catgttcttc
    12001 aactattaga tagttgtgaa ctatagcagc aattcctata ccaaataaaa ctccaccaac
    12061 tccaacagct aagccttcta agaatgtaat ttggcttcca attaatataa ctgctggtaa
    12121 tacgaaaata aatccacttg caacagattc tccaccactt gacatacctt ggattaagtt
    12181 tttaccaaga ataccctttg aacgagcaaa tgctccaaca aaagcagaac ctattatagc
    12241 tcctggaata cctgcggcaa ctgtaagacc ggctttcatt ccagagtaag ttgtagatgc
    12301 ggcaaaaatg gcagctaaaa taataccgat aatcagtaca gctacatttc caccggttct
    12361 ggacttatct gtgatatatg gaacgtaatc ttttccacta acctcaccat aagcaccctt
    12421 aggtaatttt ttgttcataa aattttcacc ccttttcaat ttgtatatat tataacatat
    12481 atacatataa aacttaactt tttattattt tacaaatagc gagtcaaaac tactaaaatt
    12541 taaccctata ttattaattt ctacttttag atatttttat gctaattttc tttttatttt
    12601 tcaattctac taaaaactat taagatataa aataaaaaaa ttttttaatg taaaattaat
    12661 tttgtaaaaa aaatgcttta aaacacggaa aagtataaaa aatacaataa tcattcttat
    12721 aaataaattt tcaccatagt aaatatatac agaaaattct tgtttttatg caaattgatt
    12781 attttataaa aataagtgtc ataaagaaat gtctgaaatc actttatgac acctatcata
    12841 ataaaaaaat tattctggta atgctctttt atttactttt ccgattggtg taactggtat
    12901 cttatctatc ctcattatta ttgttggaat catatatgga ggtagatgct catttaaata
    12961 ttctctaata gttctttcat ttatcttacc actatcttcc tcatcaatta cataatatgc
    13021 acatatataa atatctccat catcatttgt tctcgatata caaacaacac ttttaatttc
    13081 tgtaatatta tttattatat tttcaatctc atatagctct atcctaaact catcaatttt
    13141 tacttgattg tccatccttc caatataatc taaatttcca tcatctaacc atcttactaa
    13201 atcaccagtt ctatacatta acttattatg ttcacaagtt ttgtaaggat tctctacaaa
    13261 tacttgctct gttagccctt ttttatttaa atatcctctt gatatttgaa tgcctgatag
    13321 acatagctct cctggtgctc caattggaca tagtttgttc tttttatcta atacatatgc
    13381 ataagtatta aatataggtt ttcctattgg tatttttcca tattctctgt ccacatgaaa
    13441 agaagtcgat acaacagtaa attcggttgg tccatactca ttatatagtt cataattacg
    13501 ctttctatat ccttttaatc tttctcctcc agtctgtaca actctaagtg aattattatt
    13561 gcaattttgt ataaataacc ttgcaaatgc tgttggcaat gctagtatag ttatgtcatt
    13621 ttcctcacag tattcattta ctgtatattc atcaaattta gcttcttcag gcataatatc
    13681 tattgatgca cctacaagca aataagggaa tagttgtttc attacagcat caaaactgaa
    13741 acttaagtat ataccacata tatcattttc tgtagcgctt atagatttta cgctataata
    13801 acacatattt actaaactct tatgttctat agctatggtc tttggcctac cagttgaccc
    13861 tgaagtagat atcgtatatg cacaatttga tgatgttatt ttttcagtta attcatatcc
    13921 aaacttaaac atttcttcat catttatatc gagtactatg gtattctcat ttacaacctt
    13981 acttttgagt tcatctgtag ttatcactac tttcgcatta ctatcttcaa gaaggtaatt
    14041 caatctttct tctggattac ttgtgtctat tggaaaaaat gctccccctg atttcataat
    14101 ccctattgct gtaataatta tgttaatatc tcttggtaac attacagcta ctatgtcttc
    14161 actttccact cctatatgat ttaaataacc tgcaagttta tcagttactt catccaattc
    14221 tttatatgtt aactttctat tttcatccct agcagctata ttactaggtg ttaccttaac
    14281 ttgctcccta aataaatcaa cataactttt atcatagggt acttcatatt gattattatt
    14341 aaagtcttcc aataatactt tttcttcttc cttacataca aattctaaat cttctattaa
    14401 aatatcatta tcttctatta tttgctctat tatataatca aattgattta aaattcttct
    14461 aatagtttct cttttatata tagatggttt atactttatt tttattttaa gtgccctctt
    14521 agtatatact attacagata aatcatatcc ttttttttct atgtcataat catatgataa
    14581 atcaaaactt ttagctataa atattgtgtt tataagctta gtatttagat gatttttatt
    14641 ctctatatct gataacaaat agtatccata ctctaaagac ctgctccact gcctttttag
    14701 cttccttaca acatctataa atttttcact ttgtctaact cttactctta ttggaattat
    14761 attcattgtt ttaccaaaaa ctacatcatc ttcatatata tacttttgaa gtataaggcc
    14821 atatatagtt tctatgatag agtctatgct aatattgttc actttagaat attgaattaa
    14881 cctttcatgt ttctcaatat tcatcttaaa aacctcatct actacttcat cagaacaatc
    14941 tttataaaaa aaatcatttg gaatttttgc tatattcgta taatcatcta acaaatccat
    15001 ccaataattc atagctcgtt ctaaattaac ttcatacata tcaaaattcc cccttactac
    15061 taagtctata gaaaaaattc ttttggaatt ttattttcac aatatataaa tatattaata
    15121 atacatatgc aagctctaaa aacatgagct tgcataaaat tacacttatt atgactacta
    15181 aattcgtaat atttacaatg tttaaaatat attataacac atatatgatt ttatttttct
    15241 acatttatat aatatataaa ttacaaatag ttatttaact aatatataac aatacctttt
    15301 ggaatatgat atattatatt aattaaattt ttataagata agttatactt ataggaggga
    15361 accatataaa tgaatattta tgctcatagg gggtttagtg gaaagtatcc agaaaatact
    15421 attctagctt ttaaaaaatg tttggatatg gatatatatg gaatagaact tgatgtccac
    15481 agaactaagg atggaaaaat tgtagttatt catgatgaaa aggttgatag aacttttaat
    15541 ggacatggtt ttgtaaaaga ctttacttta agaaaattaa aaactctaaa ctcatctttt
    15601 gaaggatatc aaagcaataa agaatgtaaa attccaactt tagaagaagt attaattcta
    15661 atctcaccta cagaccttac tttaaatatc gaactaaaaa ctgacaaaat aaactatccc
    15721 aatatagaaa aaggcgtttt agagcttatt ttaaagtata atatgaaaaa tagagttctt
    15781 atatctagtt tcaacagcaa tagcttaaaa aactttcata aattagaccc tagtgtaaaa
    15841 acaggattat tgtgttattt acctataaac aatgtagtca attttgctaa atttctgggt
    15901 aatagttatc ttcatccacc tctagtttta gtaaatgaat ctctaattga actttgtcac
    15961 aaaaatctac ttggtgtcaa tgtatatact gtaaatgaag aggatgatat actacactgt
    16021 ctaaaactca atgtagatgg tatttttact aactatccag atattgcttc aaatctttta
    16081 cactcaaaac aatacagcta attatacaat agtttgtact ccttgacttt tatataatta
    16141 atatgtacaa ttttattaaa tttatcttgg aggtataact atggataact tattaaatga
    16201 acttgaagct ttaaagagta atttagataa tctaccaata gaaaaaatag aaaattatga
    16261 tttgagtaaa actgctttat ttataattga tgttaataat ggctttgcaa gacaaggtgc
    16321 attatactct cctagagtag agtctttaat aaagccaatt gaaatgttta caaaaaaaat
    16381 atcaaacaaa ttaaatagag taatagcatt tactgattct catactccta aatcaataga
    16441 acttttgagt tatcctgttc actgtttgga aaatgatgta gaaagtgaat tggttgatga
    16501 acttaagtcg atagaaaacc tacaaattct tcctaaaaat tctactaatg gtttttttgc
    16561 attagaaaat ttggattttg ataatataga taatataatt atagttggcg attgcacaga
    16621 tatatgtata tatcaatttg caataactct aaaatcatat tttaatcaac ataatattga
    16681 aaaaaatata gttgtaccta tgaatttagt agatacttat gatattccta acgttcaccc
    16741 tgctgagatt ttaaacttag tatttttcaa cagtatgatt caaaatggag ttaatgtatt
    16801 aaaagagatt cgttaagctt ctaatacttt acatcccata taatacaatt atcagatatt
    16861 tatttttaat aaaatttcta atagaaaaac acagttttag tgagaagatt acaatttaaa
    16921 acactatcta ttattactaa tcttcacaat ataaaaaatc gcagtttaga tattccttat
    16981 acaaatttat aaggaataat aaattgcgat ttttattttt aagtagtaca attaattgat
    17041 actacttcag tatttaaatt cattttttaa taatcatact tattcgtagt ttcgcacatt
    17101 actaccttta tttttttatt aaacatattc ttatattcta ataaaaactc atttagttct
    17161 gaatccataa gatttatttt tctatcacta caaagcatta tcttttcttg gtcgattatt
    17221 acattaatat catttaagtc attttttata aatgttgtta tacaactctt tggttggatt
    17281 ttttctccat atttctcata aaatcctgca ctcttcttaa atgactctaa atcttctgta
    17341 gatactttgc tggaagtatt attactcata aaacctgccg tagaagggtc gattccaaag
    17401 tttctaagtt gtttttctac ttcttcagtg tccataccat atctctccat aaattgcttt
    17461 tgtattttca taaatttttc ttgagatatg ttgttatctc tcatataatc gaatattttt
    17521 cttgaaaact cagccataga catctttcca tccataacag aaaaatataa gttatatatg
    17581 tattgctcaa atttatctac atcagaatct ttaactttgt tctttagttc gttaaagtct
    17641 attattctat catcagacat ttttcatcct cctagctaaa tttttacttg tataagatta
    17701 taccatattt ctattatatt tatatacaca ctattttttt aatttaacga ttttttctgt
    17761 agcatctaat tcgcacattc tgcccaaagg aatagttatc attgggatac aatgtcctga
    17821 ctgaagatta tatatagaag gtttattgac cttatttgca aattctatta gtaattccat
    17881 caaatcattt tcttcaatac aatccttaaa atctccaaaa attataccat tacattcctc
    17941 aagttttcct gaagaatata gttgtgttaa cattctatcc agtttatatt gaggttctcc
    18001 aatttcctca ataaacaaaa ttttatcttt tgcatctatt tcatattttg ttcccattgt
    18061 ggcaattatt aaggataaat ttcctcccat tattattccc tctgctttac cttcacatac
    18121 agtataaatc ttttcttctt ttggattttc taaatataat tcatctttaa attctaaagc
    18181 atttaccaat gatttatagg taaatttatc ccatttacct acatttgctg ccattattcc
    18241 atgatatggt aaaagaccac aatttttata gaaagctaaa tttaaacctg ttatatcaga
    18301 aaaacctata aatggttttg gattttgctt tattatatca taatttattt tatccaatat
    18361 tcttgttgtt ccataacctc ctcttaagca cataattata tctacatctt tatctaaaaa
    18421 catatcttct atatcccttg ctctaatatc atcattacca gctaaataac ctttataatt
    18481 taaactacaa ctttcagaaa acttaacttc ataaccataa ctttctaaat taaactttat
    18541 ttcctccaaa cttgtatttc ttaatggccc agatggcgca attattccta acatttaaac
    18601 acctcttctt ataaaatgat atattcttct ccatactaaa tatctctaat ataattataa
    18661 gatattttat ttgtacttga tacataataa tgatttacat aaaataatat aattaatgaa
    18721 gaaaaataaa tttttaatat agttaggatg tgttttatgc ttaatataaa aactctttgt
    18781 catgagataa atgattgggt tataaatata agaagagatt tacataagac tccagaactt
    18841 ggattagaag aatttcaaac taagaaaaaa attattaaat acttaaatga aataggtata
    18901 aattatattg aatataagaa tcatacaggg ataactgcat acattaacgt gagtccaaac
    18961 tttgaaacag ttgctataag agctgatata gatgccttac ctataacaga agaacttaat
    19021 tattcttata aatctattaa tattggcaaa atgcatgcat gtggtcatga tgcacacact
    19081 gctattttac tgggtacttg taatatacta tttaaattaa aggattactt aaatgtaaat
    19141 gtaaaattct tttttcaacc tgccgaggaa actattggtg gggctcaact tatgatagaa
    19201 gatggatgtc ttgaaaaccc aaatgtaaaa tatatatttg gacttcatgt aaattccaat
    19261 atcaacaaaa atctaattga attaaaatac aacactctaa atgcttccac tgatacactt
    19321 caattaactg tacatggttc aaaatgtcat ggagcttatc cacatcaagg tattgatgct
    19381 attgttatat ctgctcatat tattacagct ctacagacaa tcgttagtcg aaacacaaat
    19441 ccaactgatt ctgtggttat ttctctagga aagattgaag gtggaatcaa ggaaaatata
    19501 gtttgtgata aagtggttat aaggggtact ttgagaacct tgactcctga aactagagag
    19561 ttttcaaaaa aacgaattag agaaatttgc gattttactt gtaaaacatt tggaggaagt
    19621 atttctgttg aaattgagga aggttatcca gctcttatca attcaaacca tctagtagat
    19681 tacgtcaaac aaaatgctgt agaattgttt ggagaagaaa atattatcct taaagactcg
    19741 cccactcttg gggctgaaga cttttcgtat tttctaagac actgtgaagg agctttttac
    19801 caccttggtt gtgcaaatcg tgaaaaaaac attacttccc ctttgcatac ctctactttt
    19861 gatattgacg aagattgcct tattactggt gtcatgctcc atgtaaaaaa tgtattatca
    19921 ttctaactat taatattttt atttattatt ttatattgac cctcacatga tgtcatgtta
    19981 taccctaaaa ttacaaatga taaattaaat aattggaggt aatattatga aagaaatatt
    20041 aataaatcct ataggtaaaa tatgtattca aggcgaagag gtctttatta aattagacaa
    20101 aaagtatgta cctgcattaa atgaactcga cgatttcagt catttaaatg tattttggtg
    20161 ggctgatggg tttgataatc ctgaactaag aagtatttta gaaaccccaa aaccttataa
    20221 aagtggacca gacgttatag gaatatttgc aacaaggtca cctataagac ccaatccaat
    20281 agcattgact gctgtacaaa ttatcaatat cgaccatgaa aatggtataa taaaaatacc
    20341 atatattgat gctaatgata atagtcctgt tatagactta aagccatata ctccaagcgt
    20401 tgatagggtt gaaaatccgt ccattcctaa atggtgtagt cactggccta agagtgtaga
    20461 aaaatcagga gaatttaatt gggaagatga aattgaattt taaattactt ttttcttgat
    20521 ttcattaact tgttttccac atttatttta tttacactta ccaagatttt atgtcacata
    20581 ta
//
LOCUS       DAMJZE010000058        20561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJZE010000058 DAMJZE010000000
VERSION     DAMJZE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.058"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            79..3027
                     /locus_tag="PC541_001838"
     CDS             79..3027
                     /locus_tag="PC541_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839304.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3005..3469
                     /locus_tag="PC541_001839"
     CDS             3005..3469
                     /locus_tag="PC541_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2839305.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3582..3884
                     /locus_tag="PC541_001840"
     CDS             3582..3884
                     /locus_tag="PC541_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2839306.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3946..5310
                     /locus_tag="PC541_001841"
     CDS             3946..5310
                     /locus_tag="PC541_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2839307.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5345..5818
                     /locus_tag="PC541_001842"
     CDS             5345..5818
                     /locus_tag="PC541_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839308.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5990..6640
                     /locus_tag="PC541_001843"
     CDS             5990..6640
                     /locus_tag="PC541_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2839309.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6814..7746
                     /locus_tag="PC541_001844"
     CDS             6814..7746
                     /locus_tag="PC541_001844"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2839310.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8020..8733
                     /gene="ispD"
                     /locus_tag="PC541_001845"
     CDS             8020..8733
                     /gene="ispD"
                     /locus_tag="PC541_001845"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2839311.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8730..9215
                     /locus_tag="PC541_001846"
     CDS             8730..9215
                     /locus_tag="PC541_001846"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2839312.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9310..9518
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9582..11297
                     /locus_tag="PC541_001847"
     CDS             9582..11297
                     /locus_tag="PC541_001847"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2839313.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11419..12864
                     /locus_tag="PC541_001848"
     CDS             11419..12864
                     /locus_tag="PC541_001848"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2839314.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13302..14783
                     /locus_tag="PC541_001849"
     CDS             13302..14783
                     /locus_tag="PC541_001849"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2839315.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15004..15159
                     /locus_tag="PC541_001850"
     CDS             15004..15159
                     /locus_tag="PC541_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839316.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15304..16701
                     /locus_tag="PC541_001851"
     CDS             15304..16701
                     /locus_tag="PC541_001851"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2839317.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16686..17099
                     /locus_tag="PC541_001852"
     CDS             16686..17099
                     /locus_tag="PC541_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2839318.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17205..17963
                     /locus_tag="PC541_001853"
     CDS             17205..17963
                     /locus_tag="PC541_001853"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2839319.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17976..18713
                     /gene="rlmB"
                     /locus_tag="PC541_001854"
     CDS             17976..18713
                     /gene="rlmB"
                     /locus_tag="PC541_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2839320.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18716..19243
                     /locus_tag="PC541_001855"
     CDS             18716..19243
                     /locus_tag="PC541_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2839321.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19308..19955
                     /gene="sigH"
                     /locus_tag="PC541_001856"
     CDS             19308..19955
                     /gene="sigH"
                     /locus_tag="PC541_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2839322.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20029..>20561
                     /locus_tag="PC541_001857"
     CDS             20029..>20561
                     /locus_tag="PC541_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDF2839323.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         8508 a         2150 c         3850 g         6053 t
ORIGIN      
        1 aaaaaaataa taagtacaaa aaaaacaata tatggcatat aagttgctat ataaataata
       61 aggagggaac aattataaat gaggcaaaga ataaaaaact taattgaaaa tgaagataag
      121 aaaaatcctt taacagatga agcgatttca agtaggttaa atattcgaag agaagatgtt
      181 acctttttga gaaatgagtt aaaaatagaa gattcaagac aaagaagaaa agttgtttta
      241 attaaagcta taaaagatat tttaaaagaa gaaaaaacta taaataaaaa tgatataaca
      301 agaagattaa acagcattgg gtttacagta tcaagattta cagtcataca atacttaaaa
      361 gagattcaag attctggtga cctaaaactt gataaagaga gtgatgataa aaaagaagaa
      421 ataatcaata atagagaaaa taaagatata gcatttgata aactcatagg tagtagtggt
      481 agcttaagta caattataaa actagccaaa gcagcaattc tttatccacc tcatggtctt
      541 catactatta ttatggggcc aactggtgtt ggaaagagtg agttagcaga gtgtatgtat
      601 aaatttgcag tagaatcatc cagattccct aagaatgcac catttatagt ttttaatgca
      661 gcagattatt cagagaatcc acagcttttg ctatcacagt tatttggaca cgttaaaggt
      721 gcttttacag gagctgatga gaacaaagaa ggattagtat caaaggcaga tggaggaata
      781 ttgtttatag atgaaataca tagattacct catgaaggtc aagaaatact atttcaactg
      841 attgataaag gtatggtcag aaagttagga gaaacaagac taacacataa aatagatgta
      901 atgattattt cagcaacttc tgagccaata gattctcatt tacttaatac atttaaaaga
      961 cgtatacctg taacaattga aataccagaa ttagttgcaa gaccattaaa tgagaggttt
     1021 gacattataa ataatttctt cttaatagaa gctcaaagaa tgggagcaaa catacatata
     1081 aagtcagatg tattaaaagc tcttatgctt tacgattgta taggtaatgt aggtcaactt
     1141 agaagtgata ttcaagtagc ttgtgctagg ggactattaa accaacttac aaacaaacta
     1201 aaagaagtaa atataagcat atcagatgta cctggatatg taaaacaagg tcttataaaa
     1261 atcagaaatt gtagaggaaa aatagaaaat tatgtagatg gggatttaat tatagactca
     1321 aatcttcaag gtgagataga aaagaaaaat gaagatatat atacttttcc agatgaaata
     1381 tacaaactta ctgaaagaag acatattgat ttattggaac aaggtttaga ttttgatgtt
     1441 ataaatagaa tcattggagg agaattagaa agttgcattc aaaaatacat aaaacaagtt
     1501 aaaaaagttt ctattagtaa caatcttcca gatataagtc caattgttgg aaaatatatc
     1561 gtagagctaa cttatgaagt aatcaaaata gcatcacaat acctaagttc actagaaccg
     1621 aatctacatt tatgtttagc agttcaccta aaagcagctt ttgagagatt aaaagaagga
     1681 aaagtaatat caaatgctca tttgaaagaa ataataggaa attatccact agaatattct
     1741 gtagcagaaa aggtagctca atatttaaag gaaagatata acataaactt accaaaagaa
     1801 gaaataggta taatagcaat atattttaaa atgagtagta gaagagatat gtctgaacaa
     1861 aaaaagatag gtgtacttct tatggctcac ggaagagtag catcttctgt atgtgaggtt
     1921 acaaataaac tattaggaat taatcacgca aggtatttag atatgccatt tgagaagaaa
     1981 ccggaagaaa tgattgaaga agctactaca ataataaaag atattgatga aggtagaggt
     2041 gtgattatat tagtagatat gggttcactt tcatattttg gagatattat ttctggaaag
     2101 actggaatta atataaggac aatatctaga tttgatacat tgctttctat agaggttata
     2161 cgaagagcag ttttaccaga tttaacaata gacgagatag ttgatgaagt taaaggtata
     2221 gagccacaag taaaagaaaa ggttagtaaa tgtaataata caaaagtaga ttttagaaaa
     2281 cctataattt taacattatg tattactgga ttagggggag cttgtaagat aaaaaaaatc
     2341 ctagaggaaa atattaaaag tataactgaa aaggttgata tagaacctat aggtatgatt
     2401 gaatatccaa gtcttgattt agagataaaa caaataagca atgataaaaa tgtaatcgcc
     2461 atagttggaa caattgaccc tcagatagca aatatccctt tttttagtct agtagacata
     2521 aatgataaag caaagttaaa tcaaataaat aacctaattg atacaaatca aattggtgaa
     2581 acaaataaaa aatatgaatt atcagattta atacataaag aagatatatt tgtggattta
     2641 caggttaaat caaaagaaga agttctaaaa tatatgagta aaaaacttag agagaaaggt
     2701 tatgtgacaa aaaatttcta taaaaaagta gtggaaagag ataacttatt tccaactgaa
     2761 ttatcagatg aaatagcaat acctcacaca gatggaatag atgtaattag gccagctata
     2821 agtatagcca tcttaaagaa gcctattgtg tggagtaaaa gtaaagtaaa aataatactt
     2881 ttattagctg tagaccaaaa gtgttttaat ccattaagca ctctactctc atttatagaa
     2941 acagatgaat ttaagaagat aaaaaacatt aaagatagag attttattag ggagatgata
     3001 ttagatggag tcaatgaaaa taattagtag caatttaatt tttaaaaata ttgaagtgtc
     3061 taacaatgaa gatgcattaa agtttttggg acaacgtttg tttgacgaac agtatgtaaa
     3121 agaaagctac atacaagctg ttgtagcaag agaaaaaaag tatgctacag ggttaccaac
     3181 agaaatatac ggagtggcaa taccacatac agatatagta catgtaaatg agccaggaat
     3241 agcaattgga atactgaata aacctgtaaa atttataatg atgggtacag acgatactga
     3301 aattgatgta aaagttgtat ttatgttagc agtgaaagag ccacaagaac aattgcaact
     3361 attagaaaaa cttatgacta tatttcaaga taagaatata ctaaataata ttgttgactt
     3421 aagtgaagaa agtgtgagtg atttattaaa ctctaaattg aaaaattaaa catgtaaagt
     3481 atattaaaaa tgtaaaatta aacatgcaaa gtatgttaaa aaatataaaa agattattaa
     3541 agatttctaa ctatagatat ctttaaaata aaataatttc aatgggggtg actaatgtgg
     3601 ctaaaaagat attagttgca tgtggaacag gagtatgtac ttcaacaatt gctataaaca
     3661 aattaaagaa agcacttcaa gacattggta aattggatat ggttaatatt acacaatgta
     3721 aagtagtaga agttgcatca aaagcacctg attatgattt aataatttgt actacacaag
     3781 tatctagtag tataaaaaca ccagtgataa atggattacc ttttttaact ggtgtcggaa
     3841 tggataaatt aatcaatgat gtattagaag agttagaatt ataattaaaa aaattaagga
     3901 acatggtctc taaaatcata agttttagag acttaaaatt aaataatgga gggtctttat
     3961 atggagatat taaatttcat tgttggtcta ggtgcacaag taatgatgcc aataataata
     4021 tgtatttttg gtttgatatt aggtacaaaa ctaggaaaat ctttaagagc tggtttaact
     4081 gtgggagttg ggtttatagg tcttaacact attatagctt tgctaacaga taatttaggt
     4141 ccagctacac aacaaatggt taagaattta gggctaagtt tatcaataat agatgtagga
     4201 tggccagcag catcagctat agcttttgca tcaacagtag gggctttaat tattccaata
     4261 ggtcttgtgg taaatatagt tatgctaatt accaatacta ctcaaactgt tgatgtagac
     4321 atatgggatt actggcattt tgcttttaca ggagctttag tggcaggggc tactcaaagt
     4381 gtaatgtggg gagtatttgc agcagtagca aatatggtaa ttgtattggt aatggcagat
     4441 ttaactgctc caggtataga ggaatattta ggtatgccag gtatatctct tcctcatgga
     4501 tttacacagg catttgttcc aattgctata gtagtaaata aattattaga cttaatacca
     4561 ggcataaata agattgagat aaatgctgat acattgcaga aaaaatttgg attatttgga
     4621 gaacctttaa taatggggtc tgtaataggt gtaataattg gtattgctgc gaaatatgat
     4681 ataaaaggaa tattacaatt aggtgtaact atgggagctg tattaatatt aattccaaag
     4741 atggcagcat tattaatgga aggattatta ccagtatcag aagctgcaca ggaatttatt
     4801 gagaagagat ttaaaaacag aggcaagatt tatataggct tagattcagc agtaggaata
     4861 ggtcatcctg ttacattatc agttgcacta gtgttggttc ctttgactat acttatagca
     4921 gctatattgc caggaaataa agttttacca tttgcagatt tagcagtaat tccatttgca
     4981 cttgtattaa tcgtaccaat tacaaaggga aatgtgttta gaacattaat aataggaata
     5041 attataatta cttctggatt attaatcgca actaacttag ctccattatt tacccaaatg
     5101 gctttaaatg ctagttttaa aatgcctgaa ggagcaacta tgatttcaag tatttgtgat
     5161 ggtgcgaatc cattaagttg ggtatttgta aaagttatga actataaagt tattggagga
     5221 gtagtttttg gagttatagc tttaggaatg gctatttaca ataggaatag aataataaat
     5281 gaaaataaaa agctatctat agaagaataa ggttaaaaca attttattaa ggagtgtata
     5341 ggttatgaaa aaatataaat atgattctaa aagatactta ctcagtgtta caataccagg
     5401 attttttatg attttaatat tgatttatgc actgtttaat aactatacaa attttaattt
     5461 gaatatatat agtttgttaa taattgtctg tacttatggt atattcaata cttttatatc
     5521 atcaagtaat cctaaaaaaa ttattattga tagcaaatgt atacatttta cctcatttat
     5581 gactacacat aagtatgaaa ttaaagatat agaaaagata agaataagag aattttataa
     5641 taaaaaaatt tatctaaaaa ttaatgatgg aaaacttttc aaaggtaggt attggataag
     5701 aacaaatatg tttaataata gtatagcatt atatagctat ttcattgagt tggaagagtg
     5761 cttatatcca gattcactaa aatttagaaa taaaaaattt gagaataaga acatataaaa
     5821 ataaaaaatt gaattgaagc ctagcttaat cttttttgat aagatataag tttgaacttt
     5881 atactaaatt tttagaattt tcaatcaaga caataacata atagctaagc aattagaaca
     5941 caactaagat gtataaggca tgtatatgat gaatgggagg atttaattaa tgttattaga
     6001 gaaattaaga aaagaagtat tacaagcatc tttagacctt ttaaactata atttagtgac
     6061 attaacagga ggaaatgtaa gtgggagaga tgaacaaact ggctatatag caatcacccc
     6121 aagtggaatg gattatagaa atcttacacc aagtgatatt gttatagtag atgtagatgg
     6181 aaatattata gatggaaaat ggaaagcatc agtggattta tcagaccact tgtatatata
     6241 taagcataga gaagacataa atagtataat tcatacacat tctacatact caagttgttt
     6301 tgctatttta aatgaaccta tagaatgtgc aagtacaact cttgcaaatg aagtaggggg
     6361 ttcagtacca gttgcaaaat tttcgccacc aacatctaaa aagatgggaa aatgtgttat
     6421 tgaagcaatt ggtgacaaaa gagcatgtct tttagcaaat catggcgtaa ttgcagttgg
     6481 tccatctgta ggacatgctc ttacagccgc tgtaatgtta gaagattcag caaaagtata
     6541 ttacttagca aaatcaattg gcacaccagt gctactgcct gatgaagaaa ttcaaagagc
     6601 gagagatgta ttttttaatg tctatggtca ggataaataa attagataaa attttaaaag
     6661 taatatgaaa atatagatat tcaatgatat taaggagatt tataatgata taataagttt
     6721 aatagttaag aagtaaaata tgaaatgtaa tagtatataa attcatttaa gactaaaaag
     6781 aataagataa aataaaaagg agtctgtatt tctatgtatt acttattaaa caaaagcaca
     6841 aatccttact ttaatttggc attagaagaa tatctatttt taaatgataa atataatgat
     6901 gatattataa tcatatggag aaatgaagaa tccattttta taggtaaaaa tcaaaatcca
     6961 tatcaggaag tatatcatga tgttattgaa aagggagaaa ttcctatttt aagaagaata
     7021 tctggaggag gaactgttta tcatgactta ggtaatatca atatgtcatt catacaaaaa
     7081 gataggcaac ttcatgagat agattttttg gagcatacta agtttatgca aaagatgctt
     7141 tcaaccttag gactagatgt atctataact gaaagaaaag atttattttt aaatgggaaa
     7201 aaaatttcag gctctgcaca gtctataaaa agaaaaaatt ctctttatca tggaactctt
     7261 ctttacgatt cagatttaaa taaattaact aaatatttaa attcaaataa agcaactgaa
     7321 tcaaatgcaa ctaaatctgt ttcaagtaaa gttacaaata ttaagttttt actcgaaaaa
     7381 gatataaatt actttttaga ttattgtgtt gaatatttaa aatctaatat aaagaatata
     7441 aaagaacttg aattggatga agaagatatt aaaagtgtat acaatatagg agaagataaa
     7501 tataaaaaga tagagtggac atatggtaaa acgccgaaat ttcaagtagt gctaccattt
     7561 aataatgaaa gtaaaataaa tatacatgtt aacagatgga aagtatcaaa attttttatt
     7621 tcttatgatg ataatgtaat taatatggac aagttgttag atttgaattt ctttaaagaa
     7681 gaaataaaag agagtatttt agaaaattat ccacattaca tagatttaat caatttaata
     7741 ttttaatata aaacctctaa tttgataaaa ttgtcttata tcaaactaga ggtttttaat
     7801 attcttgcat attaacaacc attacaaaat tgtaaaatta aaaacaacac tattgaaaaa
     7861 tcaatatata ttgtaataaa tatatgtgtt tttcattagc aaatagacat taactattta
     7921 tacaactgaa aatttaatat ataattaatc agatggttaa aataaaaata ttaaaagttt
     7981 taataaatga tggttaaaaa taaaatacga ggtgatacca tgtatagtgt tataattgtt
     8041 gcagcgggaa gcggaaggag aatgaactta gatataaaca aacagttcat aaaattaaga
     8101 gaaaaagaaa ttattgctca tacaattcaa gttttttatg aaaatataaa tatagatgaa
     8161 atagtggtat gtataaaaaa agaagaagaa gattttttta aagaaaatat aataaataag
     8221 tataacttta aaaatataaa aatagcatat ggcggaaaag aaagacagga ctcaatatat
     8281 aatggtttaa aaaaattaga taaaaattgt gatatagtac ttatccatga tggagcaaga
     8341 ccatttgtcg accatagaat aatcaatgaa tctataaagg tagctaaaga aaaaaaggct
     8401 gtggttgtag gtgttccagt aagtgatact ataaagatag tgtcagatgg aacagtacaa
     8461 gaaactccag aaagaaattt gctgtgggca gcacaaaccc ctcaaacctt tgaatataat
     8521 ctcataatag atgcttatga acaagcatat aagaacaatt attatggtac tgatgattca
     8581 atgttggtag aaaatatagg tcaaagtgtc actatggtaa tgggttctta tgaaaacata
     8641 aaaataacta gtccagaaga tttaaatata gcagaacaaa tattaaatat ggaaaaaaga
     8701 gatgaagtta acagtagaag gagaattata tgagaatagg attaggatat gatgtacaca
     8761 aattaacaga ggatagaaaa cttataatcg gtggtgtaga gataccacac gataaaggtt
     8821 tgttgggtca ttctgatgct gatgtattga tacatgcaat aatggattct attttaggtg
     8881 ctctagcatt aggagatata gggaagcatt tcccagatac agatgaagaa tataagggag
     8941 cagatagtat gaaattatta gaacatgtgt ataatctaat aacaagtaaa ggatataaaa
     9001 ttggtaatat agacagtact ataattgctc aaagccctaa gatggctcca tatattgaga
     9061 gtatgagaag taatatatca aaagttttaa acacagacat tgataatata aacataaagg
     9121 caacaacaga agaaggtctt ggttttacag gagcaaaaca gggaatagct tcacaaagta
     9181 tttgtttatt attgttgaca agtcagaata attaatatat tattagtaat aagtttaatg
     9241 gatatcaagt aataggccta tatagaatat ctataatgta tgaatttaag tgaaattaat
     9301 ataaatataa aattagctat gataaagaaa tagtaaaatg atgaaatctt cacagagaga
     9361 aaacggtggt gagagttttc aagaggaagt ctttgaacct gtcttttagc ttaaaggtat
     9421 aaaaatcttt acggtaggaa ccgttatatt cccaaaagag agctattcat atagctaact
     9481 agagtggtac cgcggaaatt aaacctttcg tctctatact tgtatagaga tgagaggttt
     9541 tttatatttt caggaagtta atattcagga ggaatctatt aatgaaaatg tcaaaaatgt
     9601 ttatgccgac attaaaagaa atcccagcag atgctgagat tacaagccat caattaatgg
     9661 ttaggtcagg catgataaaa aaaatgactt caggtgttta taaccaatta ccaatggggt
     9721 taagagtgtt caaaaaaatt gaacaaataa ttagagaaga attaaataaa aaagattgtc
     9781 aagaaatact ttgtgcagcc ttacttcctt ctgaattatg gaaagagtct ggaaggtgga
     9841 ctgctatggg tgaagaaatg tttagactaa aagacagaac agaaagagag tactgtcttg
     9901 ggccaacaca tgaagaagca ttcactgata taataagaca agaaattact tcatataaac
     9961 aattgccact gaacttgtac caaatacaag taaaatatag agacgagaga agaccaagat
    10021 ttggtgttat gagaactaag acttttacaa tgaaggatgc ctatagtttt gatgtagatg
    10081 ataaaggttt agataagtca tatcaagata tgtttgatgc atatgtaagt atatttaata
    10141 gatgtggatt ggagaatagc cctgtacagg cagattcagg agctatagga ggttctacat
    10201 cagccgaatt tatggttaag tcagaagttg gtgaagatga agttgtattt tgtagtggat
    10261 gtgattatgc agccaatgta gaaagagcag aatcttgtaa tctagcatca caaaaagaag
    10321 aaatgaaaga attagaagag gttcatacac caggagcagc tacaataaaa gagttagaag
    10381 aatttttaaa aacttctcca gataagtttg ctaaaacttt agtatatgag gcagatggaa
    10441 aaacagttgt agtcgttgta agaggagata gagaagttaa tgaaatcaaa gtatctaatg
    10501 ccataggttc tgttatagaa tttgctcttg ctacagatga tgtagtgaga aaagtaacta
    10561 atgcagaagt aggatttgca ggtccaatag gtataaatgc tgactatgta tttatagata
    10621 aagaaattgt tgaacaaaga aacatagttg ttggggctaa taaaactgag taccatataa
    10681 aaaatgctaa ctatggaaga gattttgaag gaatagttgg agactttaga aatgttcaag
    10741 aaggcgacaa gtgtatagta tgtggtaagc cacttgaaat agctagaggt gttgaagtag
    10801 gacatatatt taaactagga actaagtact cagaatctat gaatgcaaac ttcatagaca
    10861 aagatggaaa atcaaagcct atagtaatgg gatgttatgg aataggtgtt gaaagaacag
    10921 cagcagctat aattgaacaa cataatgatg agaaaggtat aatatggcct ttgtcagtag
    10981 ctccatacca tgttgtgata gttccagcaa atatgaaaaa tgaagaacaa atatcaatag
    11041 ctgaaaatat atataatgat ttgcaagcta tgggtgtaga agttttatta gatgacagag
    11101 atgaaagaat aggtgtcaaa ttcaatgatt cagaattaat aggaatacct atgaggatta
    11161 cagtaggtaa aaatattaat gaaggtaaag tagaatttaa acttagacat aaagaagata
    11221 aagagataat tgacatagaa gaaataaatg aaaaagtaaa ggcagagttt atcaggaata
    11281 atgttaggtt aggataataa cttaaataag atattgtata gataatcaaa gttgtacttt
    11341 atagacacca ttttatctat atataaggat ataaaatggt gtttttttat aaaacaaata
    11401 aatgaggagg taattaaaat ggcaaaaaat gaaaaacaat ttgttgaaga aataacaaag
    11461 atggaagatg attttccaca atggtacaca gatgtaataa ctaagactga cttagttgat
    11521 tatgccccag taaaaggttt tatggttgta aagccatatg gatatgcatt atgggaaaag
    11581 atgcaagaat ttatggataa aaaatttaaa gaaactggac ataaaaactg ttattttcca
    11641 ttattaatac cagagagttt attaaacaaa gaagcagaac acgttgaagg ttttgctcca
    11701 gaagttgcat gggtaacaca tggaggaaat aaaaaattag aagaaagatt atgtgtaaga
    11761 cctacttcag agactataat atgtacaatg tatgcgaaat ggttaaaatc atatagagaa
    11821 ttaccatatc tttataatca atggtgctca gttgttagat gggaaaaatc aactagacct
    11881 ttcttaagaa catctgagtt tttatggcaa gaaggtcata cgttacatga aacagcagaa
    11941 gaagcacaag aagaaacaat acaacaatta gaagtatata aagcgctatg tgaagaatta
    12001 cttgcaatgc cagttgtagc tggtcaaaaa agtgaaagtg agaaatttgc aggtggtgaa
    12061 agaacatata ctatagaagc tatgatgcat gatggtaaag cattacaatc aggtacaagt
    12121 cacttcctag gtcaacactt tactaaggct tttgatatta cttttgcaga tagggaagga
    12181 aatttagcta atccatacca tacatcttgg ggagcatcta caagacttat aggtggactt
    12241 ataatgactc atagtgataa cagaggttta gtattaccac caagagtagc tccaattcaa
    12301 gttgttatag ttcctatagc tgctaaaaaa ggaaatgtaa tggagactgt tgataaaata
    12361 tatgctgatt taaaagctaa gggagtagct gtagaagttg atgatagaga taactataca
    12421 actggatgga aatttaatga gtgggaaatg aaaggtgttc cagtaagagt tgaaattgga
    12481 ccaaaagata tagaaaataa tcaagctatg gtatttagaa gagatacttt agaaaaggat
    12541 tctatgcctt tagaaggatt agctgatgct atatgtgatt tatttgatgt tatacacaat
    12601 gatatgtttg aaaaagcaag aaaacataga gaagacaata catctatagt tgaaaatatg
    12661 gatgagttta gaaaagcttt agaagaaaag ccaggattca taaagactat gtggtgtgga
    12721 gatgcagaat gtgaagctaa gattaaggaa gaaacaggag caactataag atgcttacct
    12781 tttgaacaag aaaacttagg tcacaagtgt gtgtactgtg gaaaagaagc agatagtatg
    12841 gtagtaatgg caaaagctta ctaaaataca tttaaataaa aactggggta gaaagtattt
    12901 aatactttat cccagttttt tgttttttat gagtaataaa atgtatttgt aactatgtca
    12961 taagataagt catagatact tatattaaaa agaatataat agagaactat ttttaataat
    13021 tacatattaa tgaaagcaaa atattttgtt atatagattt tcaataaagc aaacaataaa
    13081 tataaatgaa tattaataag gagtgctttt aatttttaga aaataaatga cttaaaagaa
    13141 agatttatga aattaaaact aggattttct gatataataa acatgtgtat ttttaaataa
    13201 aaaggatatt attattagat ttgattaaaa ataaacttgc aaaacaatgt actagtttaa
    13261 tattatagtt atattgaata aaatatcagg aggaagtaaa aatgaatgta agagtaaggt
    13321 ttgcaccaag tccaacagga tttgtacata taggtagttt gagaactgct ttatataact
    13381 atttatttgc aaaaaaaatg ggcggagaat atatattaag agtggaagat acagaccaaa
    13441 gtagattagt agaaggtgct atagagaata tgcttaatgc tatgaaatgg gctggggtca
    13501 accatagtga aggtgttata ttagatgata gtggaaaagt agtacaaaaa ggagaatatg
    13561 gtccatatat tcaatctcaa agattagata tataccaaga atatataaag caacttttag
    13621 atagtggaaa agcttattac tgtttctgta caaaagaaag actggatgaa gtaagagatg
    13681 ctcaaagagc agcaggagaa acagctaaat atgatggtca ttgtaagaat ctttctaaag
    13741 aagaagtaga agcgaatata aaagcaggaa taccatatgt tataagatta agattgccag
    13801 agaatcatac aattaagttt acagacttag ttagaggaga tatggaattt aatactaatg
    13861 acttagatga ccaagtatta atgaaaacag atggatttcc aacttatcat tttgcagtag
    13921 ttgttgatga ctatttgatg aaaattacac atgttataag aggagaagaa tgggtatcat
    13981 ctactccaaa acatgtttat ttatatgaag cttttggatg ggaagcacct gtatttgtgc
    14041 atttaccaaa tatacttaat aaagaaaaga aaaaattaag taaaagacaa ggtgatgtag
    14101 ctgttgaaga ctttaagaaa aaaggatatt taccagaggg attagtaaac tatgtagcat
    14161 tagttggttg gtctcctgaa gataatcaag aattgtttac aatggaggaa ttagaaaaag
    14221 ccttctctgt agaaagagta tctaaaagtg gtggagtatt tgatactgaa aaattaaatt
    14281 gggttaatca gcactatata aaagatggtg atgatgctta tctaacagac ttagcaatac
    14341 catttttaat agaagatgga tttataacag aagaagaagc tactaataaa tatgatttct
    14401 taaagagtat gatatcagta cttaaagaga aattacaata tgtaaaagaa gttactgaac
    14461 atgcaagtat attttttgga gataaaatag aggtagaaac tgaagaggga agagagttct
    14521 taagattaga acatatacct actttaatag atgctttgag agaaaaaata gaaaaaacag
    14581 aggtattaaa tgctgagttt gttcaagcta tgcttaaaga aatacaaaaa gagtacaaaa
    14641 taaaagggaa aaatttattt atgggctcaa gaataatttt gacagggcaa atgcatggtc
    14701 cagatttacc aaaagtaatg gaagttttag gaaaagaaac ttgcttaaat agaatagctt
    14761 atgtaaaaaa taatatatta tagtcataaa agtccgctaa tttagaagcg gacttttttt
    14821 gtgttagtat ttggtgaaaa tatctgctta agaaaatgaa ggtatttaag gtaataaatt
    14881 taatatataa aaagcaaagc aaaaatataa ataaaatatg aataataaac taataaaata
    14941 ttaagcatag aagaagggaa tctattattt tagtttgatt taattattaa gggggtaatt
    15001 actatgtata gtaatattga tgatgttaaa aaggaattaa aggagctatg cttagaatat
    15061 gttacaattt tagaaaaatt aaaagatgaa aaaatgatta cagaagaaac ttttgaaaag
    15121 tgctcttctc aaaaaaaaat atttttagaa gaacaataga ggatggtaat agtattatta
    15181 ttatgtagtt tgaaagatag tacgaaaaat gatagtaata ctattattta ttgcaatttt
    15241 caactataat ataatatata atgttaagaa gataatttat aaaaatacag tgaggtgatg
    15301 agtgtgaaag tttataatac attgactaga acaaaagaag aatttgttcc attagaagaa
    15361 ggaaaggtaa aaatgtatgt gtgtggtcca acagtttata actatataca cataggtaat
    15421 gcaagaccat ttataatatt tgatactcta agaaggtatt tagagtatag aggatatgat
    15481 gtaacttatg tacaaaactt tacagatgtt gatgataaga ttataaatag aagtcatgag
    15541 gagggaatat ctcctgaaga agttgcggct aagtatataa aagaatattt tgtagactgt
    15601 gatgggctag gaataaaaag agctactgtt catcctcagg ttacagataa tatacaacag
    15661 attatagagt ttataaaaga gttggaagat aagggatatg catatgcagt aaatggtgat
    15721 gtatattttg atacaaataa gtttgaaggt tatggaaaac tttcaggtca aaaacaagaa
    15781 gatttagagg caggggctag aattgaggtt aatgaccaaa aaagacatcc tatggacttt
    15841 gttctttgga aggcaaaaaa agaaggtgag ccaggatggg atagcccttg gggagaaggt
    15901 agaccaggat ggcatataga atgttctgtt atgtctaaga gatatttagg tgaaacaatt
    15961 gatatacatg caggtgggca agatttaaca ttcccacatc atgaaaatga aatagctcaa
    16021 agtgaagcta gaagtggaaa aacattctca aaatactgga tgcataatgg atatataaat
    16081 ataaatgatg aaaagatgag taaatcaaaa ggtaatttct ttacagtaag agatatatca
    16141 aaattatatg atttagagat agtaagattt ttcatgttat cagcacatta tagaaatcca
    16201 gtaaacttta gtgatgagat gttaaatcaa gctaaagcag gtcttgaaag actatataat
    16261 actaaagaaa aattagaatt tacgctaagt aatttagtag aatctccttt aacagaaaaa
    16321 gaagtagagc ttgttaaaga attagatgac tttagacaaa aattcataga tgctatggat
    16381 gatgatgtta atacagcaga cgcagtaagt gtaatatttg aattagctaa gttaataaat
    16441 tctaatgtag atgaaaattc ttctttggag tttgctaaaa aatgcttaga tgaatttaat
    16501 gaacttacag gagtattaaa tattgtaaat aagaaaaagg atactgtatt agataaagac
    16561 attgaagaat tgatacaaaa aagaactgat gcaaagaaaa ataaagagtt tcaattagct
    16621 gatgatatta gacaacaatt actagatatg ggaattgtat tagaagacac aagacaaggt
    16681 gttaagtgga aaaggatata aaaatggaaa aaactgaatt agttactatg tcaccattag
    16741 ttttagcata cttaggtgat acagtgtatg agacatatat aagagaatat ttaattagac
    16801 agaatacaca aagaaaagtt aatgatttgc ataaactagc tataaagtat gtaaaagcta
    16861 aagctcaggc aactataatt catgaagttg aaattgagtt aactgaagaa gagagtaaaa
    16921 tttataaaag gggaagaaat caaaagtcaa atacttctcc taagaatgca gatattatag
    16981 actacaaaca tgcaactggg tttgaggcat tagttggata tttgtattta aataatgaaa
    17041 tagagagact tcaatatatt atcaataaag gaatcaaaat tattgaaaga gatatgtaaa
    17101 tattaaagag tgttgtctta ataataagta ataaggcaac acttttttta taactaatgt
    17161 ataataaata tatagaaatt agtaatgaga aaggataaaa agttatgaaa gtaaaattaa
    17221 tatcacatac acctgaacca gaaaaagtaa tagctatggc agctaagcta tgttattcac
    17281 cagttgggac agacgaaata gaaaaagact taacagatga aagtatagag aaatttttaa
    17341 atatgttatt aagtataggt catgggtcaa tattagaaca tgcttcattt acattttcaa
    17401 tagaaggtat ttcaagggca tgttcacatc aaattgttag acatcgtata gctagttttt
    17461 cacaacaaag tcaaagatat gttaaattag aacaatttga gtacataatt cctccagaaa
    17521 tagaaaaaat agaaaaggca aaagagttat ttatagactc catgaaaaaa gaccaagaga
    17581 attatgataa gttagttgaa atattgtttg aaaatcatta taatgaccta ataaaaaacg
    17641 gtaaaaatga gaaaacagct aaaagacaag ctgagaagaa agcaatagaa gatgctagat
    17701 acgtatttcc aaatgcatgt gaaacaaaga tggtatttac tataaatgct agaagtttgt
    17761 ttaatttctt tgaacataga tgctgtgaaa gagcacagtg ggaaataaga aatttagctg
    17821 tggagatgtt aagagaggtt aaaaaggttg ctcctatatt atttaaaaag actggtccaa
    17881 gttgtgtaaa tggaagttgt ccagaaggaa ctatgacttg tggagatata gttcaagtta
    17941 gagaaaaatt taaggccttg taggaggtga ataatttggc gagtatagaa ggaagaaatc
    18001 cggtaataga agcaataaaa agcgacagag aaatagataa aatattaatt gcaaattcag
    18061 ctaaagaagg ttcaattaag aaaataatag gaatggctaa agataagaat ataattattc
    18121 agtatgttga taaacataag ttagatgaag taagtacaag tcattcacat caaggtgtaa
    18181 tagcttatgc aagtgagtat aagtattatg aattagatga gctaatagat ttagcaaaga
    18241 ataaagatga agacccattt ttcattatat tagatgagat aacagaccca cataatttgg
    18301 gttcaataat aaggacagca gatgcagtag gagcacatgg agttatcatt ccaaaaagaa
    18361 ggtctgtaca cataactcct gtagttgcca aagcatctgc tggagcagta gaatatatgc
    18421 cagtttgcaa ggtaaccaat atagttaata caataaaaag actcaaagaa gaagggctgt
    18481 ggattgctgc agctgatatg gatggagaaa ctttttataa acaaaatctt actggtcctc
    18541 ttggtgtagt aattggaagt gaaggttttg gaatatctag attggttaaa caaaactgtg
    18601 actttatagt aaaaatgcca atgataggta atgttacatc tttaaatgca tctgtagcag
    18661 gtggaatact tttatatgag atattcagac aaagactgga taaaagtaaa taggcatgaa
    18721 aaaaaatata aatcattact taataataga tggctacaat attataaatg cgtgggacaa
    18781 tttgaaagaa ctggctaaag aggacttaga agattctcga gaaaagctta ttgatgacat
    18841 tatagaattt tctgagttta tgggttataa gactataata gtttttgatg cttataatgt
    18901 taaaaattct cgagaaaaag ttgaaaaaag aaaacacata acaatagttt atacaagaga
    18961 acaccaaaca gcagatagtt atatagagaa atttataact tctttatcaa agtatgatga
    19021 tgtaaaagta gccactaatg actatgcaga acagcaaatg attttaggaa aaggtgctac
    19081 tagaatgtct gcaagagaat taaagttaga gttagatcgt tccaagaata aaatgaagga
    19141 aaaaaatatc ggtttgagga aaaaaattca gcgaaattgg ttggaggaaa ggttagataa
    19201 agaaacattg tcgaaacttg agaacattcg tagaaagcgt tgaaattact aggttcttta
    19261 gactataata tacctataaa tttgttttat atatggggga gatttacatg ttggtagcaa
    19321 aagaaaaaag ttatgagtta gtggacaatt gtcagcagga tgagtataac attgtattaa
    19381 gagcaagtga aggggataaa atagcactag agtacattat tactaaatac agaaattttg
    19441 taaaggcaaa agcaaagtca tattttttaa ttggagcaga taaagaagat ataatacaag
    19501 aaggaatgat aggcctttat aaggcagtta gagattttga tggaagtaag accaattcat
    19561 ttaaatgttt tgcggagata tgtataacaa gacaaataat aactgctata aaaactgcaa
    19621 caagacaaaa acatatacct ttaaattcat atgtttcttt gaataaacca atatatgatg
    19681 aagagtcaga cagaacactt ttagatatta tagctacaag tatagtaaca gatccagaag
    19741 agcttataat tagtaaagaa gagttaaaaa atatagaatc aaaaatgaac gaattgttaa
    19801 gtgacttgga attagaagta ctagaattat atttaaatgg aaagtcatac cagtttatag
    19861 cggacaagct taaaagagat gtaaagtcaa tagataacgc tttacaaagg gttaagagaa
    19921 aattagaaaa acatcttgaa aacagaaatg attaatagta aaatacttac cataagctta
    19981 ttgtgagcaa aattaattta tatcgaaaga taagggagga aattcaaaat ggctaaagct
    20041 aaatacgaaa gaacaaaacc tcatgttaat atagggacaa taggacacgt agaccacggt
    20101 aaaactacat taacagcagc aataacaaaa acattatatg acagatatca attaggagaa
    20161 gcagtagatt tcgcaaacat agataaagct ccagaagaaa gagaaagagg aatcacaata
    20221 tcaacagcac acgttgagta tgaaacacca aatagacact atgcacacgt tgactgccca
    20281 ggacatgctg actacgttaa gaacatgata acaggagcag cacaaatgga cggagcaata
    20341 ttagtttgtt cagcaacaga tggaccaatg ccacaaacaa gagagcatat actattatca
    20401 agacaagttg gagtaccata tatagtagta ttcttaaaca aatgtgacat ggtagatgat
    20461 gaagagttat tagagttagt agagatggaa gtaagagatt tattaacaga atatgatttc
    20521 ccaggagatg acactccaat agtaagaggt tcagcattaa t
//
LOCUS       DAMJZE010000059        20362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJZE010000059 DAMJZE010000000
VERSION     DAMJZE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.059"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..464)
                     /locus_tag="PC541_001858"
     CDS             complement(<1..464)
                     /locus_tag="PC541_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2839324.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDY"
     gene            complement(543..1406)
                     /locus_tag="PC541_001859"
     CDS             complement(543..1406)
                     /locus_tag="PC541_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDF2839325.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            complement(1667..1924)
                     /locus_tag="PC541_001860"
     CDS             complement(1667..1924)
                     /locus_tag="PC541_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2839326.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(1928..2218)
                     /locus_tag="PC541_001861"
     CDS             complement(1928..2218)
                     /locus_tag="PC541_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839327.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            complement(2249..2815)
                     /locus_tag="PC541_001862"
     CDS             complement(2249..2815)
                     /locus_tag="PC541_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839328.1"
                     /translation="MTFKELVNKVRNLVLEAKKVTIEDTENKFTSENVEEALKECINR
                     ADEAFQEADSGKELIATAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            complement(2849..3031)
                     /locus_tag="PC541_001863"
     CDS             complement(2849..3031)
                     /locus_tag="PC541_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839329.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAIED
                     YIIKNSGVVEGAEPTI"
     gene            complement(3031..3336)
                     /locus_tag="PC541_001864"
     CDS             complement(3031..3336)
                     /locus_tag="PC541_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839330.1"
                     /translation="MTEYVEVGRRIFFDEEGKIIFYEGQSKGNVPDRKNIKKIEYIDL
                     EYDYVDYDKYKIIGIDIRTKQPILEEIPVYMSEEGKRIQELENQILIAENEKSGGLL"
     gene            complement(3360..3965)
                     /locus_tag="PC541_001865"
     CDS             complement(3360..3965)
                     /locus_tag="PC541_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839331.1"
                     /translation="MIEKLNENASLSDLITTFENVKDELQIGKNNIASTLGNPFVGTD
                     KLSVTKTKIQTLKNTLAISLSMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGE
                     KTFQLYQVDNNTPKTCSANAISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITA
                     VPQPSNFGEMYMPNTFDCVVGHTCILPVKDYKIPHFFYAIA"
     gene            complement(4176..4310)
                     /locus_tag="PC541_001866"
     CDS             complement(4176..4310)
                     /locus_tag="PC541_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF2839332.1"
                     /translation="MWYKIIKKYYEDELWTKEQVKTAVEKNKITTIEYKNIVGEDYIV
                     "
     gene            complement(4311..4676)
                     /locus_tag="PC541_001867"
     CDS             complement(4311..4676)
                     /locus_tag="PC541_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839333.1"
                     /translation="MIKIWDKKEKINGVSAEEILKGNYDFQTSEVFLILDDYNRVTNI
                     ESVNTIKSIYKLDKNLTALETAEKYLEMHKKQEEDIKAHEELEKNSKNATIATHEEVK
                     ALREETATLTFAMMEKELI"
     gene            complement(4694..5827)
                     /locus_tag="PC541_001868"
     CDS             complement(4694..5827)
                     /locus_tag="PC541_001868"
                     /inference="COORDINATES: protein motif:HMM:NF025330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HDF2839334.1"
                     /translation="MTIKLEEDSSIKEVMDSFENIKNDLQNAKDNLASTLGSPFVSTD
                     SFSTTKTKIQTFKSTLATNLTKKGVSTSSNETVQNMVSKVDDIELGNTDIPNWYKPKN
                     IIFDGVTSALQQYPPCVAVENYIYFLCNNSLGLSLLRYDTLTNVNSVLAKPSVCDDAL
                     MVYYDGYIYRIGGYSNSSANSTCQKYNIKTNTWSYISNMITPRGGTCGNTYSNEIHVL
                     YGYTDTNSNSTASAISEYYKISTNTWSTKTSFPPSRDRRLFVCAKTGTYYFCITGATD
                     PNNIWETYINRYDAGNGALTKIEGWYKSFATSINDMLYTVYYGDTYLYVKGYNQNGRV
                     FSKEYVKKNSTFPYNPLLKAVSVKNKYIYYYENNMYKCFIPEL"
     gene            complement(6020..7165)
                     /locus_tag="PC541_001869"
     CDS             complement(6020..7165)
                     /locus_tag="PC541_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HDF2839335.1"
                     /translation="MATKLEENAKLREVIDTLETTKDDLQTVKNNIADVLGLPFTGND
                     KLETTKNTLSSIRSTFVSNLSKKGVSTVSNAPFKTLAENVGKIEQGNMIVPPWYVPKN
                     IFIKALSTAVKDKTPCVSAEKKIYFINGYTGTSGLNNLCYDTVLNTTTTKNIPSSFVE
                     SALFFYDGYIYKIGGFSGYSAPTAICQKYNILTSSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSTYKIVVFGL
                     KSSISSHYFSTINYDPVSGTYSYAGEGFDSNTFAVQLGSTLYVSDYGYKAGSGKCGVV
                     IFSSSGIKKLTFDKGAVPNYSYFGASVDGRQIYFILNSEITCFIPEL"
     gene            complement(7182..8258)
                     /locus_tag="PC541_001870"
     CDS             complement(7182..8258)
                     /locus_tag="PC541_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2839336.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEARSELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDRFTSDKLDGVLE
                     EIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(8270..8887)
                     /locus_tag="PC541_001871"
     CDS             complement(8270..8887)
                     /locus_tag="PC541_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2839337.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(8880..9929)
                     /locus_tag="PC541_001872"
     CDS             complement(8880..9929)
                     /locus_tag="PC541_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2839338.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(9930..10349)
                     /locus_tag="PC541_001873"
     CDS             complement(9930..10349)
                     /locus_tag="PC541_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2839339.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(10354..10614)
                     /locus_tag="PC541_001874"
     CDS             complement(10354..10614)
                     /locus_tag="PC541_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2839340.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            complement(10628..12520)
                     /locus_tag="PC541_001875"
     CDS             complement(10628..12520)
                     /locus_tag="PC541_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2839341.1"
                     /translation="MADDLVLANDRDVRLIIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(12513..13214)
                     /locus_tag="PC541_001876"
     CDS             complement(12513..13214)
                     /locus_tag="PC541_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2839342.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWLICKKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLKLG"
     gene            complement(13230..15623)
                     /locus_tag="PC541_001877"
     CDS             complement(13230..15623)
                     /locus_tag="PC541_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2839343.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITTGTVLIIQGLGWITNAALNVANVFAQNWSIIAPAVYG
                     VVAAIAIYKGILLASAIVTGVSSFVNALYALGAYKACAALAAQELALFGKISAQTIEA
                     MVTAQATAAQYGLNAALYACPLVWIVLGFIAVIAVIFMVVAAINKFAGTSLTVLGVIV
                     GAVFAAVATIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DSVNNAGNALGKAFVDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNRWVGDAPEKVKLERMGYKDIGAEFQKGNALGTKWQNAISNKLKDTFDINK
                     MLEDAKDKLGLKDLWDKDNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(15688..16203)
                     /locus_tag="PC541_001878"
     CDS             complement(15688..16203)
                     /locus_tag="PC541_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2839344.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREEE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     repeat_region   16334..17221
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16399..16427
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(17297..17446)
                     /locus_tag="PC541_001879"
     CDS             complement(17297..17446)
                     /locus_tag="PC541_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839345.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKREK"
     gene            complement(17498..18313)
                     /locus_tag="PC541_001880"
     CDS             complement(17498..18313)
                     /locus_tag="PC541_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HDF2839346.1"
                     /translation="MMNELMNFEGKDIEVFEFEGQILFNPKHVAECLDISDVNSSIRK
                     FNDNQVVKLTNSKMHNMQFRKLHNTGENFLTESGVYKLIFKSKKEEAERFQDWISDEV
                     LPAIRQTGAYITNNADPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLTA
                     KTLYKKAGIDLPIEINEEEHYFDTKQIASKLKLYSKSNKPAQMAVCEIIKKIDLEDSE
                     VKGVWETNGSWTGTVNKYTESVIDKIRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            complement(18431..18613)
                     /locus_tag="PC541_001881"
     CDS             complement(18431..18613)
                     /locus_tag="PC541_001881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDF2839347.1"
                     /translation="MSNKLGRPPKENSKKLRFEVRLNQEQADILNECAENLKISKTDV
                     VIRGIELVKEKIDKNK"
     gene            complement(18800..19324)
                     /locus_tag="PC541_001882"
     CDS             complement(18800..19324)
                     /locus_tag="PC541_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839348.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
BASE COUNT         6256 a         3738 c         2272 g         8096 t
ORIGIN      
        1 tcataatctt ctttattatc acagaagaaa ctttcaatta atactgctgt aggatttgaa
       61 ctatttaaga tatataatcc tttatctaat ttagctcttc tatttctaaa tactgtacct
      121 agcttattac atattctagt tgcatattct aaacctttat tactataata tagaacttct
      181 gaaccttttc cttgaccatc acttgcattt agatgtagtt ctataagtaa atcatatcct
      241 ccactattaa ctctaggtat tttataagtc ttttcttctg ctttagtttt aaactgcttt
      301 tcagggcata ttattacatc tgccttatga ccctcttttc taaatgtatc tgctaatact
      361 ggtgcaagag atttattgta ttggtattcg ttaactactc catcagcaga agtgcatgca
      421 ccacttttta aaatactgtg tcctactgta atacatattt tcattattta ttttcctcct
      481 tcttttcaat aaaaaaacac ttacatatag taagtgttta taaacttctt aaagtaacta
      541 ttttatttag ccaatatctc tatccaatta ttaggaaaac caataagctt taagtctata
      601 gaattattat aatcattaat taacttttga agttttataa aaaaactatt ccaaattaat
      661 ttatccatag tcaaatgctt catagcaagt atataagaaa atatcttttt attatccaag
      721 ttatactttt tatattcgtt ttttattgtt ataatacgga aattattatt atatattcta
      781 ccataatgag cgcattgatt tctgacctgt gttaatgatt gtaaccaaga tttaactaat
      841 aaagtattca ctctacacaa attatttttt atgtatctag tatcttctgg caacatgttt
      901 gaatataatt ttgacaacat accaaaagtc attatttccg ttgcaaccca aatgggaagt
      961 tttccttcat atttttcttt atggtgtata ataaaaagct tatctgaatt attacttttt
     1021 tctctctcta atgctgtcaa aaaattaata tagaattttt catctttgaa actctccctt
     1081 tctagatatc cacaagcacc atgtttaatt gctaatgtat atgcaatgta tgtcctaaat
     1141 gctatttcta tgctccctaa caaatctgtt aataatatcc taaattcctt attaaaccta
     1201 tatatatcat aaacttcttc aaacgtagtt ccttctttat atgagccatc atcattctta
     1261 aaacttagaa gatatgctgt aaacctataa taattgacat tacttaatac aaatttagca
     1321 tcttctttat catttattat taatcctcta ctttttaaaa tatctatttg ttcatcaaat
     1381 gttttttctt cttttacttc aaccattgtt ttcacctttt catagatata cttttatata
     1441 ataaaaccca ccaattaata ttagtgggta aatttatctg tcccgcctat ttgagctata
     1501 aaagaagctt ggcgggttcc ttcaaaaatc aatgtcccgt atatttgagc atataaatgc
     1561 tttacgagtt ctgtactatt attatatact tattagctat aaaagtatac tctatttaat
     1621 gaaatttttt aaatttttgt gagtaataat atttttgtta atttcattac ttttcaccat
     1681 ccttcaactg tttgtaagtt tgatttatac ctattgcaac tccccaacaa attacacctt
     1741 gtaagactgc aacaggattt aatcctagca tccatattga gaaacctatt ccaagtatca
     1801 gtaacactac tggaatgtat ttattatcta attgtttata tttcttgaat ccaacaccta
     1861 aaacataaag agcagctact aaaattagca actgctctgg tataaaactt attaaattgt
     1921 ccatctttta ttttcctcca attctattta taatttcatc aattctaaga tgtgcttgtt
     1981 ttgtacttgc ttctactttt gcaactcgtt cactgatatc taagaaactt gtattaaact
     2041 ttgctacatc atgctttata tctcttacat tctcacacag aaaagttatt tgttgttcta
     2101 attttgtagt tgtttctgta tcatcttgta tctttctgtt tgagctattt ctaaatgcta
     2161 agtaggatat tacaactcct gcaactgcac ataatagatt tatgctaatt tcttccatac
     2221 atcctcctag ttttaataat ataatttatt atgcgaaaca agcaatataa taacctgtaa
     2281 aaggatatgg ctcataacta gtatcatatg aatgaactga aaaaccagtt ttattgctgt
     2341 ctatagtaat ccatgcacgt tcagaacctc ctgtattact tcctaaagaa ctaccactgg
     2401 gattataata atatacactt cttctaaagt aaacatagac aataaggtaa cttgcactct
     2461 tgccaaaatt agcagaaaaa ggattaccat catatccatc aataggacca tatctaatag
     2521 aatatttgct tttcaattga gtttctaaat tgcttatatt gctcttaaat cctgtaatat
     2581 agttcgcata atcttgaaat gtttgttctg atgtagcagg agagccgata gcagttgcaa
     2641 ttaactcttt tccactatcg gcttcttgaa aagcctcatc tgctctattt atacattctt
     2701 tcaatgcttc ttctacattt tcacttgtaa acttattttc tgtatcttct atggttactt
     2761 tctttgcttc taatacaaga tttctaactt tattaactaa ctctttaaaa gtcatttagt
     2821 caccttcttt caataaaaaa agaaccctct atatagttgg ttctgctcct tctactactc
     2881 cactattttt tataatataa tcctctatag cctttctgta ctctgtgtta gtcacgtcat
     2941 ctaactgaaa ttctctattt ttcaatgggt ttaaccctcc gtttaatatt ctctctgcta
     3001 atattcttac tacaacatta tttatattca ttataatagt cctcctgatt tttcattttc
     3061 tgcaatcaat atttgatttt ctaattcttg tattctcttt ccttcttcac tcatatatac
     3121 tggtatttct tctaagattg gttgcttagt tcttatatct attcctatta ttttgtactt
     3181 atcataatca acataatcgt attccaaatc tatatattct atttttttta tattttttct
     3241 atctggaaca tttcctttcg attgtccttc atagaatatt attttgcctt cttcatcaaa
     3301 aaatattctt cttcctacct ctacatattc agtcatagtt tcctccttta acttattttt
     3361 taagcaattg catagaaaaa atgtggtatt ttgtaatctt tcactggcaa aatacaagtg
     3421 tgacctacta cacaatcgaa tgtgttaggc atgtacattt caccaaaatt gctaggttga
     3481 ggcactgctg ttataatatt acgaaaagat gagtaaaaaa caaaactaca caaacctata
     3541 ttgtaagcat ttctatagac aataataaat tttggagtaa aagctaagtt aaaacttatt
     3601 gcgtttgctg aacaagtttt tggcgtatta ttatctactt gatagagttg aaaagttttt
     3661 tctccaactt gagtacattc tccttgtgca ataaaaatat ttgcgattcc agctacttta
     3721 ccaattagtg aatgaagtgt ttcagttgat tgagcagata tatttttcat agacaaacta
     3781 attgctagtg tatttttcaa ggtctgtatt ttggttttag ttacacttag cttatcagtt
     3841 ccaacaaaag gatttcctaa tgtactagct atattatttt taccaatttg caattcatct
     3901 ttcacgttct caaaagttgt tattaagtca cttagactag cattttcgtt taatttttct
     3961 atcataaaaa tatcaccttt ctatttttag taactgtttg tcaagaaatg gttacttttt
     4021 aaaatagaaa agtgattgat tttaagtaat tttttcaaag tatatatagt ataaataatt
     4081 ttatttttaa tagaaaaaca agcaaaataa acaagtataa agctttttag taactctttc
     4141 ttgacaaata gttactatat aaaattaaaa aaggactata caatgtaatc ctctccaacg
     4201 atatttttat attctattgt tgttatctta ttcttttcta ctgctgtttt aacttgctct
     4261 ttagtccata attcatcctc atagtatttt tttattattt tataccacat ctatatcaac
     4321 tccttttcca tcattgcaaa agttaatgtt gctgtttctt ctcttaatgc ttttacttct
     4381 tcgtgtgtag ctatagttgc atttttagaa tttttttcta gttcctcatg tgcttttata
     4441 tcttcttctt gtttcttatg catttctaaa tatttttctg ctgtttctaa agcagtcaaa
     4501 ttcttatcta gtttatatat agatttaatt gtattaactg actctatatt tgtaactctg
     4561 ttataatcat caagtattaa aaatacttca cttgtttgaa aatcataatt accttttaaa
     4621 atttcttctg ctgaaactcc atttattttt tcttttttat cccatatctt tatcattttt
     4681 tattttctcc tttttataat tcaggtataa agcatttata catattattc tcatagtaat
     4741 agatatattt attcttcact gaaactgctt taagtagtgg attgtatgga aatgtagaat
     4801 tttttttaac atattcttta gaaaatactc ttccattttg attgtatcct tttacataaa
     4861 gatatgtatc gccataatac acagtatata acatgtcatt aatacttgtt gcaaaacttt
     4921 tataccaacc ttctattttt gtaagagctc catttcctgc atcatatcta tttatataag
     4981 tttcccaaat attatttggg tctgttgctc ctgttataca aaaataatag gtgcctgttt
     5041 ttgcacatac aaataatctt ctatctcgag atggcggaaa gcttgtttta gttgaccagg
     5101 tatttgtact aattttataa tattcactga tagcacttgc tgttgaatta ctatttgtat
     5161 ctgtgtaacc atacaaaaca tgtatttcat tagaataagt attcccgcat gttccacctc
     5221 taggtgtaat catattagaa atatacgacc aagtatttgt ttttatatta tatttttggc
     5281 aagttgaatt tgccgaacta ttgctatatc cacctatcct gtaaatataa ccatcataat
     5341 aaaccattaa agcgtcatca caaaccgatg gtttcgctaa gacactatta acatttgtca
     5401 atgtatcata gcgtaaaagc gataaaccta aactgttatt gcaaagaaaa tatatatagt
     5461 tttctactgc tacacatgga ggatattgtt gtaatgccga agtaactcca tcaaaaatta
     5521 tatttttagg tttataccag tttggtatgt ctgtatttcc tagttcaata tcatcaactt
     5581 tacttaccat attttggacg gtttcatttg aagaagttga aacacctttt ttagttaaat
     5641 tagttgctaa tgtactcttg aaagtttgta tttttgtttt agtagtacta aatgaatcag
     5701 ttgaaacaaa aggacttcct aatgtactag ctaagttgtc tttagcattt tgaaggtcat
     5761 tttttatatt ctcaaaagag tccataactt cttttatact tgaatcttct tctaatttta
     5821 tagtcattaa attcctgcct ccttttttaa gtatcctttc gtttagaaat ggttacttaa
     5881 aaaagtaaaa gggacagaaa tttaaactat tctaaaaaca gctttatatt ttaaattaat
     5941 ttactttaat atacaatgta gtaaattcaa agattacaaa agtattaagt aaccatttct
     6001 aaacaaatag ttactaattt tataattctg gtataaaaca agtaatctca ctatttaaaa
     6061 taaaatatat ttgcctacca tcaactgaag ctccaaaata actataatta ggaacagctc
     6121 ctttatcaaa agttaatttt ttaattccac tactagaaaa aataacaact ccacatttgc
     6181 cacttccagc tttataacca taatctgata catataaagt tgaacccaat tgtacagcaa
     6241 aagtattact atcaaagcct tctcctgcat aactataagt tccacttaca gggtcgtaat
     6301 taattgttga aaaatagtga gaagaaatag aactttttag cccaaacaca acaattttat
     6361 aagtgctgac tttagcgcaa acaaaataat gcgattgttt tgaatctatt atagttgatt
     6421 ttgtagacca agtgtttaat gttgtattat aatattcaac aactctgact gcttgagatg
     6481 aagaagaatc ttctccgcca tacaaaacat gtatttgatt tgaatacatt tcacaacaag
     6541 cacttcctct tgcggtaggc atatttgcta tttcagtcca agaactagtt aaaatattat
     6601 atttttgaca tatagctgtg ggagctgaat atccactaaa accacctatt ttatagatat
     6661 atccatcata aaaaaatagc gctgattcta caaaggaaga aggaatattt ttagttgttg
     6721 ttgtgtttaa aacagtatca tagcaaagat tatttaaacc tgatgtacct gtataaccgt
     6781 ttataaagta aatctttttt tctgccgaaa cacatggtgt tttatcttta accgcagtag
     6841 acaaagcttt aataaatata tttttaggaa cataccaagg tggaacaatc atgttgcctt
     6901 gctctatttt accaacattt tcagctaacg ttttaaaagg tgcattactc acagttgata
     6961 cacctttttt acttagattt gaaacaaaag tacttcgtat tgaacttaat gtattcttag
     7021 ttgtttctaa tttgtcattt cctgtgaaag gcaaacctag aacatcagct atattatttt
     7081 tgactgtctg caaatcatct ttcgtggttt ctaatgtatc tatcacttct cttagttttg
     7141 cattttcttc taatttcgtt gccatttttc actctccttt attaaatcat atctattaaa
     7201 ttattcacta tagttattcc tttagttctt tgaccactta tttctaccat tatttcctct
     7261 aatactccat ctaacttatc acttgtaaat ctatcattag catctgtaat acttatactg
     7321 gtatctataa gctgtatact gctaatagaa tcctctattt tcttagacga ataagtagtc
     7381 atttcagaca ctctgttatc atccacagtt gcattaataa aatgagtttc tgcatttcca
     7441 tttatcacat aaacgtttaa ttctgacctt gcttcacttc taatttctat agaattatca
     7501 tctataattt taaagtttgt aactacattt tcttttgtag tagcatctat aatatttaca
     7561 actattctct gtgttaataa actatgtgtt acagttgctt tgaatccact ttctgcatcc
     7621 tcaacccaat catcaattgc gaccctttga gtgtatgcaa catttgagcc acctgcgatt
     7681 aattggtcga ttttagtatt taactctgtt ttagcagttt ctatgttgct tgttaattct
     7741 gttttagttg tatcaatttt agtattaaca gtacctattt tagtttctaa gtcttgtata
     7801 tctttgagtg ttgcaaagat tattgttggg tcaattttaa gttctatatt atttacatta
     7861 gatacaataa gcacagtttt aaccttcatg tctaccactg caccttgttc tatagaaggt
     7921 ttataacact ctttgtattt agaaatggca attaaattat tttcatcatc taaatatcct
     7981 atttctctta tcataaaccc gcctacactt gatggtatta aactctctaa tattatacaa
     8041 tttggtgcag tttcatctgt agttgtattt ccaatattgc cttcccatac cacgtttttg
     8101 agagctgtct gactctcagt tggagtatat tcactccctc ctccatcacc aagttgaatt
     8161 tttacaaatc ccactttatt acctgtgaca cttgcatttg ctatctttgc tttccctaca
     8221 tctgtaatta tagtgtaata acttttatct atagccaata tatcacctcc taaaatattg
     8281 ttatctcttg gtatccaact ccattgccag ttaatacatc aatttctcca taagtttcta
     8341 tatctggtgg actccaaggg tatatagtta tttcttgacc tattagggtt gttataccaa
     8401 aattcatata attgtcttta cttacaagca ctctagtgta atctaaagtc atgttgcaag
     8461 gtttaatact acttacaaaa gaatgaacct cctcaaacca atcttgattt ctagcatcac
     8521 tttcaagatg tatattataa gtagcattat taatagttaa ttcataattg ccttctccaa
     8581 ctatactatc tagccagttc cttaaaaatc tctctgagta aggtagttta cttatatatt
     8641 tactaaaaat cctaaacctt ctatcttcta aactctcatt acttttagga gttatagaca
     8701 ttatcttttc ccatcttttt ataccacttg gagttaaatc ctctaaaaac tggtcacttg
     8761 ataggtcatt taatttttca tgtagtgttt ttatttcttt gttttctaca ttaaatactt
     8821 ttatatattc ttctttatct tgtagaattt gtggtaagta atttattaga ttaatctctt
     8881 tatccaacta cctcacctct cactactata ctgttactat ctattgttag attagattta
     8941 acctcattta tcattgtatt tgcaatgtct aatactccat caatactaag taatctagtt
     9001 tcaatttgag atatacggac tattaagttt tcttcatctt cccaactcat gttaagttca
     9061 tttaaatagt cgtctattgc ttcttctgca attgatttta tattctccca agtgtagcca
     9121 tttttgtatg ttatctctgc tgatatattt atagttgtac ttgtaactcc ttcaactgtg
     9181 actcggtgtc caattggtgc taatccaaga ccttctcctt ggttttgtag aggatcaatt
     9241 tcttcttgaa ctaaattaac taaatcacta gatggaactt tgaagttaga attaattatt
     9301 actaacttaa cagtacctcc accgtcccac acaggataaa ccttaactcc tccaacatct
     9361 tgtattttgt taacttcatc tttataattt tgcatatttc caccaaaact ctgtgagttt
     9421 aggctatcat aatatctttg tcttaaacta tcttcacttt cttcatcttc tccattaatt
     9481 agtatttcag ttaactcagc agtttctaat ttgtctacat attcaattgg aatcaactga
     9541 ccaagttcaa atacaggtcc agtagtttca catttcattt tatatgtttt ttcagatatt
     9601 ctctcaattg ccacataatt gtattctcct aagttgaatc ttgaatcaat aggaatatct
     9661 atattaaaaa ctccttttgc aattgtattg gttgcaggta aaggtgtaat acctctttct
     9721 ttacatctct tctctaaata gtaataacta gcagtatcta caaaagtttg gtctagtaat
     9781 tcatccatgg caatgtatgt ttctgtaagt tctatagcaa caggagcaag agcattatat
     9841 attatagaac cttccctttt atcaaaagta tctggtacac tatctaacat tcttttaatt
     9901 atattttcaa atgtcattaa ctcaaacaat tatacactca ccaccttctc tgcttttata
     9961 tttccatatt tactgtaaac tgtgaactta caatgtactt taccctttac attttgaaac
    10021 tcaaaattat ctacattttc aaccctatca tcttgaatta gtgcttcttt gattcgtctt
    10081 tcaagttcgg ggattacaaa ggatataggc tctccaataa ggtcgttcaa ctcgactcca
    10141 taattccaac tatatattag atgttggtat ctctctgtgt ttaaaattaa aaagatggtt
    10201 tgttttaatg cttcaacatc atcacaaata ccatctatct tagatttttc tatatttaat
    10261 ttaaaggtct tacttggttc ttgccttaca tcaaaattaa ttattgatac atcttcaatg
    10321 tcataatcta aattatcgct tggtaacact tcatcacatc ctatccaata ctaaatattg
    10381 ttgtcctcct tgcatacgaa ttaagactac tttatctcct atttttttat ctgtatatct
    10441 tttaaatgta tctgtttgta ttagaaaaat ttcaccaata gatagttttt gttctatctt
    10501 aactcttaga ggattaatac tttctattgt tccaaataca accctcattg ggttgcttgt
    10561 ttctactgca tccattgcag ctttttttat tatctgtaat aaatcttggc tcatattgtc
    10621 acctcactta tataaatctt ctcacatgtg tgtatgcttt tccttttcta taagaattaa
    10681 cagactctat ttttaccaca tctcctgttt gtggtgaatg aattatttga ttgtttccaa
    10741 tatacattac aacatgatta ctacttcctc caccaattct acataataag tctcctgctt
    10801 tccacttgct tctatctttt aaatctactg cttttcctgc tttactttgt gcagaagcgg
    10861 tacgaggaat ttttatacct atttgtttat aacaccattg agtaaaccca ctgcaatcaa
    10921 aattattagg accttctgct ccatacacat aattacaacc cagtttactt tttgctatac
    10981 taattaattt atcttcttta gagttattat ttgtactact ttggttatta ccttcaactt
    11041 gatatgtttg ttcttcatct ccacctataa ttatatagcc attctttcta ccaaattttt
    11101 tacattcact agcattagct aatagtatat ctatatgata tgttccgttt gtttcaacat
    11161 atattcttcc tccattatct ttaactgtat atactttgtt gtcataggca gtaccaggaa
    11221 gtataatttt tactttatct ccatattcaa aaactggatg tttctttaga aaatcatcag
    11281 tataccaagt tttcttaact ccttctcgat tcattggacc agcaacagtt cttgatttta
    11341 catcaagtgg ctttccattg caatctgttt ttccgccttc cattgcattg ttccctggat
    11401 aatatgcagt aaatatagca ggaacttttt tacctgtatt ttttttcgta atactttgtg
    11461 caggaccatt tttcttttca tctttattat tagtatttcc actactatat gagcttgaag
    11521 aatatgaagc aaattcgtct ccatcaacaa gggtcaaatc cataaagtgc gaattatttt
    11581 caaatgtatg ttttactttc tcaactaaca tataattttg taattcaata tctcccaaat
    11641 ctaaaaaaac aggtactaaa caacctgctc tcactctaat atcaccaagt acatttttta
    11701 aacttaatga cttagttttc ttattatata gttttagaag tatatcacac ttttgtttta
    11761 tctctgcttc actcatgttt ttgtctactg tatcaaacat ttgaagtatt ccccaactcc
    11821 tcatatgcgt tgagtcttga gcaatataca catctctttt tcctgtttct tcattatctc
    11881 tcacaagttt aatctttgtg taagtatcac tatctattga tgaattatag tcaaaatctt
    11941 ctattacatc attgttcata acagtatcta atttcattga tgcaacattc tttaatgtta
    12001 ttcttccaaa atcatcatat aacacataca tttctttttt ctctcttaga gtatcatcta
    12061 gtgctgttag tatcatgtca aagagtgttt tattttcttc gactctagat agtttatact
    12121 tagtatcttc tatgacattg tattttaaat taaaatcttt agccaacatt tttacaagtt
    12181 cacttgctgt tttattgcta tacacataag tatctttgtt cttaaaatat cttaactggt
    12241 cgtaagcaac aattttaatg tgattttctt tatctctttt cttctgaaat atatatccat
    12301 agaatatacc tacacctttg taatatagtc ttacagagtt accttcacaa aactctaata
    12361 tatcatccat gactattgta aattcaagct tagatggtgt tcctcgtctc tctatttccc
    12421 atgtgatacc atcaatgaca gcaggttcat agaaatcttc ccaatgtgct atgattaatc
    12481 ttacatctct atcattcgcc agaactaaat catcagccaa gttttaacac ctgccctttg
    12541 taaatagtgt atttacttaa gtttttgccc ttatttgcct tatccatcat agatttattt
    12601 agttcgtata ctttcttata taatgaacca ttaccaagtt gcttcttgca gattaaccaa
    12661 aggctatccc ctgcttttac tgtatatgtt ttaccatttg gcttattgga tgaatctggt
    12721 ctaaattctt ttggtttcat gactggaggg ggagtcctac catagtttgt cttttcagga
    12781 gttgcaggaa ctaacttttt agttgagtaa tctctatatt gctttaactt tattgcaact
    12841 tttgtatctg agccattttc tgcatcttct actatgttat attcctcaag agatactttc
    12901 atattagtgt taaatagtac tttattacct aattccctcg atacaataaa ttgaaatggc
    12961 ttacaatcag tttttagtaa ttctagttta cttaaaaaga attgaacatc tctaaattga
    13021 cctctacaaa aaggtaattt attatgtgta aattctgctt caaaacttat ttcagataat
    13081 ccttcttttt ttaatatatt tacttctcca acatttatta aatcaactgt cttgttttta
    13141 tttgtaactt taatctctaa ctttggcgga ggtattggta attgtactcc atctaaataa
    13201 aagtcataag ccatttatat ccctcctctc taaactattc cctcagctga tacaaccata
    13261 gcgtcgttta atttttctgt tagtacattt actattccat ctacatctgc ctcactattt
    13321 atgttgtttg tattgttcat gtcaatttta atgtttactc ctgtgaatct atttattgtt
    13381 tcttgctctg caatatctct aagatatttt aagtcttctt gacttttatc cattgtttta
    13441 gccattttgg cagtatttcc cgccgtgtcc tttacatttt ttcctaaatc tccaccaaat
    13501 cctccaagat tattaagtgg attgtcttta tcccacaaat cttttaatcc tagcttatct
    13561 tttgcatctt ctagcatctt attaatgtca aaagtatctt ttaatttatt agatatagca
    13621 ttttgccatt tagttccaag tgcatttcct ttttgaaatt ctgctccaat atctttgtat
    13681 cccattcgct ccaattttac tttctctggt gcgtctccta cccatctgtt taagctatca
    13741 atctgttgtt tgatgtaact attatctgcc ttaactggcg taaacgttgc ctctcctact
    13801 ttaccaatat ttatccctgg tattttattt attaggtcaa ttaacttatt tacccctctt
    13861 attgctatat ttgctccatc cacaaaagcc ttaccaagtg cgtttcctgc attatttaca
    13921 gaatcattca atgatgccat tttctcaatt ataaaaatta caccttttgc aattgcttgt
    13981 ttcatcaaat atacacactg attccatcca ttcgcaattc cttcatttac atctacacat
    14041 ctattaaata gccatatcat tatattttgt attgttgcaa ctgctgcaaa tactgcacct
    14101 acaatcaccc ctagaacagt caaagacgta cctgcaaatt tatttattgc cgctactacc
    14161 ataaatatta cagctattac tgctataaat ccaagaacta tccacacgag cgggcaagca
    14221 tacaacgctg catttaggcc atattgtgct gctgttgcct gggctgttac catcgcttct
    14281 atagtttgtg cagatatttt accaaataaa gctaattctt gtgctgccaa tgctgcacaa
    14341 gctttatatg cccctaaagc atacagggca tttacaaaag atgatactcc agttacaatt
    14401 gcacttgcaa gtaaaattcc tttatatatt gcaatagctg cgacaactcc ataaacagca
    14461 ggtgcaataa tactccaatt ttgagcaaag acattagcaa catttagtgc agcattagta
    14521 atccatccca atccttgtat tatcagtaca gttcctgttg taattccttg tacaaaatct
    14581 gaaaaaaaat cactatttaa tatatttttt ataatattta atgtatcata aatgtttgaa
    14641 gatattgcaa tcataacatt ccgcatagat acaacaatct cactaaaacg tacactttgc
    14701 aaagctccgc ttatcttact gagagtctca gaaaatatca tataagcatc atttttcatc
    14761 attgtgagag cttgagaaaa agtgattggc atacttgcaa atttcttttc tatctcatca
    14821 gaagctttaa acagtgcatt tcttataaca tctgctgtta ttgctccgct acttgataat
    14881 tccttcaatt ggtctttagt ttttcccatt gcatcagcta ttttagtggc taataagggt
    14941 gcattttcca ttatggaacg gaactcatca ccttgaagtt ttcctgcacc catggcttgt
    15001 gttaattgat acattgctgc actagcttca tttgctgatg ttcctccaat tacaaaagct
    15061 ttattcataa gttctgtaaa ctttatcacc tctgctgaac ttccaaaagc atctcctgca
    15121 agtattccaa gtttagcaat ctgtgctgct gtatctgcat aactagctct tgcactttgg
    15181 gctgacaaat aaatcatttt attaagctgg tctgttgtct gtaagccatc attcattaga
    15241 tttaaacgtg cttttgtact tgcaattgta tctgctgctt ttgtaatact ttctattcct
    15301 tttattccaa tgtatatccc agcaatactt tttaatttag acactaatag agaacctgct
    15361 tcactacctt gccttatttt attattaaag ttttcctgct catttgtatt tcttccaatt
    15421 ttctgttcta tccttgtgag aatactttct atgttattca aactttgttg agaagcttgt
    15481 atattacctg cattgagtgg attattcaat ctgccttgca atctctctaa actattaatt
    15541 gttgtattaa tagaattagt catattacga aatgctggtg tcattccgtc gaaaatcttt
    15601 attgaagttt gtattgtagc catttcctca ctctcctttc ataaatttcc atataaaaaa
    15661 acacctacat tagtaagtgt ttattattta tgcccaaaac tgtttaccac agctcaaaca
    15721 tgtaactcta actttctttg ctcctaaatt tccagctact aaaccaatgc ctcctgctat
    15781 agtagcccct gctacagctt ttcctatgcc aaaacctttc ttatgagcag acaatgaaac
    15841 agagccacac ctagggcaac gagcaacatc atcttcaagt acattagttt gtttatactc
    15901 ctcttccctc aatatctctt tttcctcttc gatatttatt ttttcactta attctttaaa
    15961 agctggatat ataatatttt tacactcttt tatgtccagc cctagtaact tcattgcaat
    16021 atcaattgct aagattttat ctcctttagt ttctttataa atattagtca gatttaattc
    16081 ttttccatga acatttataa ataaatcatc attgttaact ttatctgcta atgtagctgt
    16141 tccgcaattt gagcaaaatt tgcctgcacc agttatctca taaccacaat ttgaacaaaa
    16201 cacaacaatc acctcacata atacttattt tatagcacta ttatactata tacgtaaaat
    16261 ttttacatta ttatcacctc ctttcataaa aaaaaacact tactcatttg taagtgtttt
    16321 tgaattattt ttaattttaa gtccacatag ttaatataaa acgagcatca tttatcattg
    16381 ctaatcgtgc atttgtacat ttacatacca catagttaat ataaaaccat acactagatt
    16441 ctaccccaaa aatgaagctc aatatattta cataccactt agttaatata aaacgcgtta
    16501 atgcagtacc ataacgatat gggactaact aatttacata ccacatagtt aatataaaac
    16561 cgtataggaa tgaatgcagg tagtgcattt tctagtacat ttacatacca catagttaat
    16621 ataaaacgca acccctaaag accttctaaa aaaattactc ttagatttac ataccacata
    16681 gttaatataa aacacaggtg tatttaccta tagaacaaca agagttacat ttacatacca
    16741 cttagttaat ataaaacatt caagaagtaa ctgtaattgt tcatctgtaa aataatttac
    16801 attccatata gttaatctaa aacctggtct taaaaacata catgttgcaa aagtgaatga
    16861 tgaatttaca ttccatatag ttaatctaaa acgaacacta acaaaaaagc taaaagtggt
    16921 cactatatta tttacattcc atatagttaa tctaaaacga atgatacatt agaatatata
    16981 ttttgtagtg aatgatttac ataccactta gttaatataa aaccatccta cttagatact
    17041 gcacaaatag tatcgcgaat ctttacatac cacttagtta atataaaatt gaccagtacg
    17101 aatgcgaggc tagcaatgat aaacgacttt acataccaca ttgttaatat aaaaaccttg
    17161 agtgacgcag caaaaggggc aaaggataca gcatttacat accacttagt taatataact
    17221 cctacctata ttatatcatt ttttactaca taaagcactt gaaacagtaa aatattcaag
    17281 tgctttattt gtctatttat ttttctcttt ttttcctaca ttcttctttc actaaactta
    17341 caaatctata aaattcatct ggattttcat ttttcatttt ttcaaagata tttccaagtt
    17401 ctttgataag ttctattcta tctatatcaa gtaaattctc atgcattgta ttaatccccc
    17461 caaaactaaa ctaaaatatt taatacaact gataaattta ctcaatttta taaaccacat
    17521 gataattctt cttctcacct gcaatcttgg taggtctatt attctcctct atccaatttc
    17581 ttattttatc tattacactt tctgtatact tatttacagt accagtccaa cttccattag
    17641 tctcccaaac tcccttaact tcactatctt ctaaatcaat ctttttaata atctcacaaa
    17701 cagccatctg agctggttta ttactcttag aatatagttt taacttagat gctatttgtt
    17761 ttgtgtcaaa ataatgttct tcttcgttta tctctattgg caaatcaatt cctgcctttt
    17821 tatataatgt tttagctgta agtaacttag atttattgtc aaaacctgca ccatccaata
    17881 gttcttttaa catagatgtg ctgttataag ccagttgtaa tttttcaatc tcacttgctt
    17941 tttctcttag tttttcgggg tcagcattat ttgttatgta tgcaccagtt tgtcgaatgg
    18001 ctggaagtac ttcatcactt atccaatctt gaaatctctc agcttcttct tttttagatt
    18061 taaatataag tttataaaca ccactttcag ttagaaaatt ttctcctgta ttatgcaatt
    18121 ttctaaactg catattatgc attttagaat tagtcaactt aactacttga ttatcattaa
    18181 attttctaat actactgttc acatctgaaa tatctaaaca ctctgccaca tgctttggat
    18241 taaataaaat ttgcccttca aattcgaata cctctatatc ttttccttca aaattcatta
    18301 attcattcat aataaattcc tccttaaatt tgattgtaag aagtacctta ctatgataga
    18361 atatatttca tagaaggtaa cttctttggg aaacagtcgc aagtgctttg gtcggtgcag
    18421 cggctgtttt ttatttgttt ttgtcaatct tctctttcac caattcaatt cctctaatta
    18481 caacatctgt tttagatatt ttaagatttt cagcacactc atttaatata tctgcttgtt
    18541 cttggttaag tctaacttca aatcttaatt ttttggaatt ttcttttggt ggtctgccta
    18601 atttattgga catcttatca cctctctttt tattgtccgt acttaaatta taatatagta
    18661 cgtacaataa ttcaagagtt ttttactaat tttttctaat tattttactc aaccgaccaa
    18721 ttttgagcaa aacaaaagca cctaccaaaa agtaagtgct ttctatataa cagatttaat
    18781 tttaaaataa agtattcacc taatttccag acgagaaatc caaatcatca attaaattta
    18841 cattattttt tatattattc caatcatcta aaaatacatt ataagtgagt gtcacatcaa
    18901 caataaagct tattctcact gaatggatat cattcatatc ttttatattg tttacaaatt
    18961 ctttagtaaa gtttctacac tcattttcat cattaaacct agaattttcc acttgaatac
    19021 taactatata tccctcacct tttgttgggt ttaatatgtt aacataatat gttgttttat
    19081 cttttaagtc ttctggtatc atagagtcta cttttgcttg catctcttgt ttataaatat
    19141 cttctttttc tctttcgcta attatatttt cttttgtaat atttccttca tttacttcat
    19201 ctctattttt tgaatctcgt atcgcttgat gaatcatcat tgatactgta aatacaacaa
    19261 agtatattaa aaatatcact aaaattttct tcaacaaact taatttttta aattttcccc
    19321 acataatgaa ctctccccta ttaaattctt tattcaaata taattctatc taaaaataag
    19381 aacttagtct attgatttaa aatattttga gcaaaataaa agcacctacc aaaaataagt
    19441 gcttctttct tctatttagt ttttctccac attgttaata taaaagtttg gagttttaaa
    19501 attatcattc ctatcttttg ctttatttac ataccacata gttaatataa aattgaggta
    19561 gaaaatgata aaaagacttt tagagtaatc tttacatacc acatagttaa tataaaacct
    19621 gttaatgtaa tcatttcacc atcataagtt gcgaaattta cattccatat agttaatcta
    19681 aaacaataat tggtgatggt acaggatata caaatgcaac atttacattc catatagtta
    19741 atctaaaaca gaaaattcat tgtacaattc ttctttgtat tttttaattt acattccata
    19801 tagttaatct aaaaccccaa aataaactta gtatttccaa tatctacaca tacacacctc
    19861 tctcaaattt gcagtgaacc atgagtagtg caattgataa catttatcac acaccctcaa
    19921 taccttgtat tccaattgtt aaacttcaat ttatcgcaaa tattgctcac tgcaaaatct
    19981 ctacattttt attatatcat aaaaatatta tttttgaata tctgtaccaa tttgtggtat
    20041 aataaaagca aggaaataat ttactttata caagagtagc tatttccatc aaaattgatt
    20101 taaagaatta tttttttaaa tcacccttat tggcgtctgg gtgatttttt attttgtcat
    20161 aaatataagc tgatataaca ccagctagta tgcttaataa aaaaactatc atataatttc
    20221 acctccttcc ttatttggaa tttggcgttt aatatgaaaa taatcaccct tcgcactttc
    20281 gattattatc cttgctacaa ttattataac atataattat tacatatttt tccatttttt
    20341 ttatataaac aatgaaattc aa
//
LOCUS       DAMJZE010000060        20243 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJZE010000060 DAMJZE010000000
VERSION     DAMJZE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.060"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            132..587
                     /locus_tag="PC541_001883"
     CDS             132..587
                     /locus_tag="PC541_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2839349.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            636..1220
                     /locus_tag="PC541_001884"
     CDS             636..1220
                     /locus_tag="PC541_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2839350.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1225..1548)
                     /locus_tag="PC541_001885"
     CDS             complement(1225..1548)
                     /locus_tag="PC541_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839351.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1700..2218
                     /locus_tag="PC541_001886"
     CDS             1700..2218
                     /locus_tag="PC541_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2839352.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2394..3062)
                     /locus_tag="PC541_001887"
     CDS             complement(2394..3062)
                     /locus_tag="PC541_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839353.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3177..4592
                     /locus_tag="PC541_001888"
     CDS             3177..4592
                     /locus_tag="PC541_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2839354.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4815..5273
                     /locus_tag="PC541_001889"
     CDS             4815..5273
                     /locus_tag="PC541_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839355.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5270..5854
                     /gene="clpP"
                     /locus_tag="PC541_001890"
     CDS             5270..5854
                     /gene="clpP"
                     /locus_tag="PC541_001890"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2839356.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6166..7242
                     /locus_tag="PC541_001891"
     CDS             6166..7242
                     /locus_tag="PC541_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2839357.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7309..9384
                     /gene="bclA3"
                     /locus_tag="PC541_001892"
     CDS             7309..9384
                     /gene="bclA3"
                     /locus_tag="PC541_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2839358.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            9553..10314
                     /locus_tag="PC541_001893"
     CDS             9553..10314
                     /locus_tag="PC541_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2839359.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10623..10901
                     /locus_tag="PC541_001894"
     CDS             10623..10901
                     /locus_tag="PC541_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839360.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11194..11769
                     /locus_tag="PC541_001895"
     CDS             11194..11769
                     /locus_tag="PC541_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2839361.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12208..12918
                     /locus_tag="PC541_001896"
     CDS             12208..12918
                     /locus_tag="PC541_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839362.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            12912..14228
                     /locus_tag="PC541_001897"
     CDS             12912..14228
                     /locus_tag="PC541_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2839363.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            14240..14380
                     /locus_tag="PC541_001898"
     CDS             14240..14380
                     /locus_tag="PC541_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2839364.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            14439..15020
                     /locus_tag="PC541_001899"
     CDS             14439..15020
                     /locus_tag="PC541_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2839365.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            15167..16876
                     /locus_tag="PC541_001900"
     CDS             15167..16876
                     /locus_tag="PC541_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839366.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            16891..17763
                     /locus_tag="PC541_001901"
     CDS             16891..17763
                     /locus_tag="PC541_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2839367.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            17764..18552
                     /locus_tag="PC541_001902"
     CDS             17764..18552
                     /locus_tag="PC541_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839368.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(18648..19481)
                     /locus_tag="PC541_001903"
     CDS             complement(18648..19481)
                     /locus_tag="PC541_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2839369.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(19549..20178)
                     /locus_tag="PC541_001904"
     CDS             complement(19549..20178)
                     /locus_tag="PC541_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2839370.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         7903 a         2315 c         3379 g         6646 t
ORIGIN      
        1 gattaaagaa aactaaaatt ttaaggaaaa ataacatatt tgtattaaat tttattgaaa
       61 tgattaaatt tatttactta atgtaaatta ttagctaaaa ttgtattaaa taattaagga
      121 ggtgagtagc tctggatgaa atagatttaa aaataattaa ttctctaaaa aagaatagta
      181 gggcttcaac ctctgaaata agcagacaaa ttaatttatc agtaccagca gtctcagagc
      241 gtataaggaa aattgagcag tcaaatttaa ttcaaaagta cactattcaa attaatcggg
      301 aaaaaacaaa atataagctt ttggctttta tatttatcaa tactgattta tctaaaagta
      361 tgaaagaatt taagaaaact atattggaat atgatactgt attagaatgt catcatattt
      421 caggagagta tgattactta ataaaagtat tagcagaaga tacaagtaat cttgaagagt
      481 ttatatcagt gactttgaag gaaattgggg ggattcaaaa agctaataca accattgttt
      541 tatctacaat taaagaagaa tttaatacaa taaaagatat tttttaaata atttactagt
      601 aaagtgttaa ttatatttta ggaggaaaat agaatatgaa agttttagtt tttttagcaa
      661 aaggttttga aactatggaa tttagtgtat ttgtagatgt tatgggatgg gctcgtaacg
      721 attatgggca tgatattgat gtagtgactt gtggatttaa aaaacaagtt atgagcacat
      781 ttaatataca agtattagtt gataagacga tagaagaagt atgtgttgat gattatgatg
      841 ctctagctat tcctggggga tttgaagaat ttggatttta tgatgaagca tatgattcat
      901 cttttttaaa tctaataagg gagtttaatt caaaagaaaa aataatcgct tctatttgtg
      961 tggcagcatt gccagttgga aaaagtggtg ttttaaaaaa tcgtaaagct actacatatc
     1021 atttgaaaaa tggtaaaaga caaagacagt taagtgagtt tgatgtaaat gtagttaatg
     1081 aaccaattgt tgtagataaa aatattataa cctcatattg tccagaaaca gctccacatg
     1141 tagcttttaa gcttttagaa atgttgacat ctaaagaaca aatggatgaa gttaaattag
     1201 caatgggatt taaactgtaa tggtttatag attttctaag taatcagaac cccagtcaca
     1261 catggaatct aaaattggaa taatactctt tccaaaatca gatagcgagt attctacttt
     1321 tggaggaaca acaggataaa ccactcttat aattaaacca tctgattcta attcacgtaa
     1381 ttgttgagtt aacatcttag gcgttgcttt aggaattaat ttttttaatt cattgaacct
     1441 caatatagtg tctttaagat gccaaagtat aagtgtctta tatttaccac caatcaatgc
     1501 aagtgttgct tctattgggc aactgtaatt atctaaacat tttttcattt ttgggctcct
     1561 ttatttattt taaagtatct ttttaggtac tatattacaa aaaagtgcat tattgtaata
     1621 tagtaccata gtgataaaat tatagcatag gttaaataaa tcatcaactt agcctaataa
     1681 aacaatagga ggtaaataaa tgaatttcgt tgaacttgca aaaaaaagat attcttgccg
     1741 taattatcaa gatagaaagg ttgaaaaaga aaaacttgag aaggttttag atgttgcaag
     1801 aatagctcca actggtggta atcgtcaacc tcaaagattg attgttattc aggaaaaaga
     1861 aggtataaat aaactttcta aggctgccaa tatatatgat gctccattag caattcttgt
     1921 atgtggagat aaagacaaag tttggacaag accttttgat gggaaacaac ttacagatat
     1981 tgacacaagt atagttacag accatatgat gttacaggca actgaacttg gattggcaag
     2041 tgtttgggtg tgctatttta atccagatat aattagagaa gagtttagtt tgccagataa
     2101 tctagaacca ataaatatat tattgatggg atatgaatct aagataccag agagcccaga
     2161 gagacatgaa aaaactcgtg ttcctttaag tgaaattgtt tcttatgaaa ctctttaatt
     2221 ataaaattaa gttattcata ggataaatgt aataaagtgt ttattataaa caaaattcaa
     2281 aataaataat atctacatat aattaatttt agtgaagtat atctagttta actagaatgt
     2341 atacttcact aatttattta ttcaaaatat ttatgaggat tttaatatat taactaaaat
     2401 atatcaattg aattaacttt taaatctgga aaagcacttg aaatatagtg ttttatgtca
     2461 tttaaatggt cttgaatttg cttttctgat attttatcag gtccatatac aaaatacata
     2521 atatttttag tatcttttgt tatagctgtt cgattatcag ggccattaag aatactagat
     2581 aatataccaa tattatctga taaaaataaa tcatcttttg ttaaaatatg ttctttacca
     2641 cctataccat aaaaatgttc acttccattt gctaattcta ttttcaaagg taattccatt
     2701 ttatcaaggt catgacctgc tgttagaaga agatttttca attctgctaa aaacattgac
     2761 tcaattgtta cacctacatc tgggatacct tttgatttta atagaataga ttctaattga
     2821 agcaatacag ggtaagtttt cttgaatttt ttataatact ttatataact tgaaacaggc
     2881 tctgttttaa ttttttcttt acgattgtat tctgggtgtt gttctataat atttttaatt
     2941 gtagagtttt taagaactaa aaaattttct ttattaggtt ttgaatgtgt aatatttata
     3001 accataactc caaatttaat attgggataa atttctttaa ctttatgtga tatttctaac
     3061 ataatataga ctccttttta ttttatattt tatgatataa tcttagcaaa ttaaaagtgg
     3121 attttgtagt tccattttta tagtaatttt atagaaccac ttttggaggt attagaatgt
     3181 ggattacgat taatagaaat agtaatatat cattatcaag acaaatttat tgtgagataa
     3241 aagatatgat tctggatgga agcttaaagt ttgagcaaaa actgccatca tctaggactt
     3301 tatcaaaaga actatctgta tctcgtaata cagttttaga tgcatataat caattaattg
     3361 cagaaggata tttggaaaca aggaatggat atggaacaat tgtagctaaa ggaatatcag
     3421 ggcataaagt tgattttaag ttaaatgata cttttagaga tgaaaaagat ttacatacta
     3481 ataaacaaaa aaattatata gattttcgtt caggagttcc agaattaaaa ctatttccac
     3541 gcaaagaact ttcaaagcta tataaacaaa tactgtatga tttatcagat tcggatttca
     3601 gatatggaag tacagctggt gtatgggttt taagagtgga aatatctaag tatttatttc
     3661 gtactagagg aattagatgt aatcccaaaa acattatgat tgtttctggt tcaacacaag
     3721 gtttatcttt aatttcacag cttttataca aaagtaataa aaaggttgct gtagaagacc
     3781 ctattcataa aggcttatta aaggttattt catctattgg atattcagtt gttggaatta
     3841 aagtagatga taagggaatg aataccaact taataaaatc aataaagggg atttcttttg
     3901 tttatactac accttctcat caatacccat taggagggat tttacctatt caaagaagac
     3961 tagagcttat taaatgtgca acagagaatg attactatat aattgaagat gattatgata
     4021 gtgaatttag atatgagggt caaccaatta gttctcttta tgaacttagt ccaaataaag
     4081 taatttacat aggttctttt agtaaaatat tatctccagc tattcgtgtt ggatttatgt
     4141 tattaccaga caatatttta gaaagctata aaaccttaaa aatgtactct gatgttcata
     4201 cagaaacaat attgcaacat gtattagctg aatttattgg aaatggaggt ttagagaagt
     4261 acatatttag aatgaagaag ttgtacaata gaaaacgtaa gtacctcata aaagttttaa
     4321 acacggaatt taaaaatgaa tttaaaataa aaggtgaagc agcagggctt catatagttg
     4381 ctcatttcta taatgttaaa tttacggaaa gtttaattca taaaattttt gaagcaggta
     4441 taaaagttta tcctatacaa caatatgcaa ttgagaatat attagaatat gactatgaaa
     4501 ttatactagg ttatgctcat cttagttttg atgaaattga aaaaggtgta aaaatactca
     4561 gcaaaataat acatatgaat caaggtacat agataaaaca tgtagcaatc tacatataat
     4621 agatgtaatt aagtttaaac tttaaaagaa atatatatta atatttttgt ataaaaatac
     4681 ctgatatttt tttatcgata ctttattgct atacactaaa ttaagatagc aatttatatt
     4741 aagaaaaaac tttttgcata ggttataata cttaaaagga gaggatagag ttgaaaacaa
     4801 atacagatgt tattatggag ataattgatt atatagaaga aaatcttaca gaaaatctaa
     4861 atttagataa aatagcttat attgctaatt attcaaaata ccatttacac agaatgttta
     4921 caaatgttgt aggatgtact atccatcaat acatacagag gaggagactt actgaagcag
     4981 ctagacaact tatatttaca gacaaatata ttatagacat agctattaca gtaggatatt
     5041 ctacacatca atcttttaca ctagcattta gaaatttata taaagaatct ccacaagtat
     5101 atagaaataa agatgaattt tatcctattc aacagagatt tgttttaaat ctaaatcatc
     5161 aaattcaaag tgaaaaatgt atttcttatg caaatatgaa ttataaacaa agaagagatg
     5221 aaatcaattc ttatctgaac tttaatatta attgtgaggt gaaagtagca tgagtttagt
     5281 gccacatgta attgaacaaa caggtcaagg ggaaaggtct tatgatatat attcaaaatt
     5341 attaaaagat agaattatat ttataggaga agaaattaat gatgctattg caagtttagt
     5401 agtagctcaa ttactgtttt tagaatcaga agacccagat tcagatataa ttatatatat
     5461 aaatagccct ggtggctctg ctacatcagg atttgctata tatgatacta tgaattatgt
     5521 cagatgtgat gtatcgacta tttgtattgg tatggctgca agcatgagtg cttttttact
     5581 tgcaggagga acacatggaa aaagatgtgc tttacccaat tcagagatta tgattcatca
     5641 gccaatggga ggagctaaag gtcaagcaac agatgtaaaa atagctgtag ataatatatt
     5701 gaaaataaaa gaacgattag ataaaattct ttctgaaaat acaggtaaat caatagaaga
     5761 tattaggaga gatacagata gagataattt tatgactgca ctagaggcta aagagtacgg
     5821 gctgattgat tatataatga ataggaatga ataaagtatc aaagtatcat attaaaatgt
     5881 ctgttttaca aatatatata taatattaaa taagagtgtc tcaaaatgaa ctttttagtt
     5941 tatgggacac tcttttttgt atgaaattaa atatggtttt atcatttcga caattattac
     6001 caaatttcaa gtttaataaa ttctactata aaaaatacat aaaagataag gttctttagt
     6061 aactttctgt cgtcaaaagt ttttataaat ttaataacaa aacaagagaa aataccatat
     6121 attacttatg tctgcaatat atagaagaca aaggagttgt acattatgat tacaataagt
     6181 ttgtgcatga ttgttaagaa tgaggaagaa gttataggca gatgcttaga atgtgtaaag
     6241 gatatagtag acgaaattat aattgtagat accggttcta cagattccac aaaagaaata
     6301 gtctcaaaat atacagacat ggtgtacgat ttcgaatgga tagatgattt ctctgctgca
     6361 agaaattttt cattttctaa agcttctaaa gactatacta tgtggcttga tgcagatgac
     6421 ataattttag aagctgatag agaaaaatta ttaaagatta aagaaagctt ggatacttcc
     6481 attgatattg ttatggcaaa atacaatgta tcctttgatg aaaatggtaa cccaactctc
     6541 tcatattata gagaacgatt gtttaaaaga agttctaatc ataaatgggt tgaccctatt
     6601 catgaagtag tacctctttt tggagaggta ttttattctg acattgcaat atctcataaa
     6661 aagcttcata gacaggaccc tctaagaaat cttagaattt ttgaaaaaat gatttcagaa
     6721 ggaaaaacat tggagccaag acaccaattc tattattcta gagagttgta ctataatgct
     6781 aggtataaag aagcaataga aggttttact aagtttttag actcatcaag aggatggatt
     6841 gaggattgta tcagtgcttg cagagattta gccacatgtt attacttaat taatgatgaa
     6901 aaaagtgcct tatattcatt gtttagaagt ttcgaatttg atgagccaag agcagaaatt
     6961 tgttgtgaca taggaaagca tatgtttgat agacaaaaat ataaagaagc cattttttgg
     7021 tataaggtag cattaacaag agataagaat gatactgatg gaggctttaa gtcagatgat
     7081 tgttatggat atataccatc tatacaatta agtgtctgtt atgacaggtt aggagaatca
     7141 gataaagcta tttattatca tgaaaaaaca aaagaaatca aacctaatga tagtgctgtt
     7201 ttgcacaatg aaaattattt tagtaaattt aaaaatagtt gatgggtcac aatcatacta
     7261 aatttgacat attatatata gagcttaaaa gctcaaatat atcaggaggt gcttttaata
     7321 atgagtagaa ataaatattt tggaccattt gatgataatg attacaacaa tggctatgat
     7381 aaatatgatg attgtaacaa tggtcgtgat gattataata gctgtgattg ccatcattgc
     7441 tgtccaccat catgtgtagg tccaacaggc ccaatgggtc caagaggtag aaccggccca
     7501 acaggtccaa cgggtccaac aggtccaggt gtaggggcaa caggcccaac aggaccaacc
     7561 ggtccgactg gtccaacagg aaatacaggg aatacaggag caacaggatt aagaggtcca
     7621 acaggagcaa caggggcaac aggcccaaca ggagcgacag gagctatagg atttggagta
     7681 acaggtccaa caggcccaac aggagcgaca ggagcaacag gagcagatgg agtaacaggt
     7741 ccaacaggtc caacgggagc aacaggagca gatggaataa caggtccaac aggagcaaca
     7801 ggggcaacag gatttggagt aacaggtcca acaggcccaa caggagcaac aggagtagga
     7861 gtaacaggag caacaggatt aataggtcca acaggagcaa caggaacacc tggagcgaca
     7921 ggcccaacag gagcagtagg agcaacagga ataggaataa caggtccaac aggtccaaca
     7981 ggagcaacag gagcagacgg agcaacagga gtaacaggtc caacaggggc aacaggggca
     8041 acaggagcag atggagtaac aggtccaaca ggggcaacag gagcaacagg atttggagtg
     8101 acaggcccaa caggtccaac aggagcaaca ggaataggaa taacaggagc aacagggtta
     8161 ataggtccaa ccggagcaac gggagcaaca ggagcaacag gcccaacagg agtaacaggg
     8221 gcaacaggag cagcaggact aataggacca accggggcaa caggagcaac cggagcagat
     8281 ggagcaaccg gagcgacagg ggcaacagga gcaacaggtc caacaggagc agatggatta
     8341 gtaggtccaa caggagcaac aggagcaaca ggagcagatg gattagtagg cccaacaggt
     8401 ccaacagggg caacaggagt aggaataaca ggagcaacag gagcaacagg agcgacaggt
     8461 ccaacagggg cagatggatt agtaggtcca acaggagcga cgggagcaac aggagcagat
     8521 ggagtagcag gtccaacagg agcaacagga gcaacaggaa atacaggagc agatggagca
     8581 acaggtccaa caggggcaac aggtccaaca ggagcagacg gattagtagg tccaacagga
     8641 gcaacaggag caacaggatt agcaggagca acaggagcaa caggcccaat aggagcaaca
     8701 ggtccaacag gagcagatgg agcaacaggg gcaacaggag caacaggtcc aacaggggca
     8761 gatggattag taggtccaac aggagcaacc ggagcaaccg gagcgaccgg agcaacaggt
     8821 ccaacaggag caacaggagc aacaggagca acaggtccaa caggtgctag tgcaataata
     8881 ccttttgcat caggtatacc actatcactt acaactatag ctggaggatt agtaggtaca
     8941 ccaggttttg ttggctttgg tagttcagct ccaggattaa gtatagttgg tggagtaata
     9001 gaccttacaa acgcagcagg aacattgact aactttgcat tttcaatgcc aagagctgga
     9061 acaataacat ctatttcagc atacttcagt acaacagcag cactttcact tgttggctca
     9121 acagttacaa ttacagcaac actttaccaa tctactgcac caaataactc atttacagct
     9181 gtaccaggag cgacagttac actagctcca ccacttacag gaatattatc agttggttca
     9241 atttctagtg gaattgtaac aggattaaat atagcagcaa cagcagaaac tcgattctta
     9301 ctagtattta ctgcaacagc ttcaggtctt tcattagtta atactgtagc aggatatgca
     9361 agtgcaggaa ttgcaataaa ttagattaga taagaaaagg cttctaatat cgtattgata
     9421 ttaggagctt ttttaagaat gattgatgaa catagtttta atattaatta gtgcgcatat
     9481 tataaaatac tattatataa tataaaataa taacattaaa ttggaaatat agaattatct
     9541 aggggtgata ctatgaaaaa taagattgaa atatcaatga acaactttgt aaaaaattat
     9601 cataaatcaa ataatataac tacaaagtgg caagaacttt tgtttgctta tgcatctgct
     9661 gaagatgata tgtttcagaa gctaaaaagt gttgtaggaa catctcatgc tatgccaaaa
     9721 gattttctgg aaaatggaaa aactataatt acatatttta taccttttga tgaaagtgta
     9781 aataatagta atattggagg gtttgaatgt tctgaaatat gggcaagagc gtacatagaa
     9841 actaatatgt tgatttcaaa tttaaatgat ttcttatgtg aagaactaga gaagatgggg
     9901 gctaattcat atgctattcc tgggtcgtat aattttgaca gtgaaaaatt ggtaagtgac
     9961 tggtcacaaa gacatgtagc attcatagca ggtcttggaa cttttggttt aaataatatg
    10021 cttattacag aaaaagggtg ttgtggaaga ataggaagca taatcacaag tttagaatta
    10081 gagccaacta aaagaccaga aaatgaatac tgcttgtata aacataataa tagttgcaaa
    10141 aaatgtgtca acaaatgcgt gaaagaagct ttaaaagaag atggatttaa taggaataag
    10201 tgttatgaaa tgtgtatgat aaatgaaaaa aaatacactt atataggtgg atgtgatatt
    10261 tgtggaaagt gtatagttgg agttccttgt tcagtaataa acccatcaag ataatggatt
    10321 taagttgact aataatttta aagatagttt ataatgaaaa aattttagtt atcaaaataa
    10381 atataatata aaaactaaat aaatgtgttt aaaaaatgga taaataattt gtgttgattg
    10441 acaaccagtt atactgttga tagacttata tgtatcgctg tgatatataa ggagggttga
    10501 gattttatgt ttgatttaat ctttattttt gcatagcaag ggctattgca actatgaaat
    10561 ttaatatata aatagccctt tgctattcct aatgaaaaat tactatttaa taggagagca
    10621 aaatgagcta taaaaaagca atacatatat taccagaaga attacttgaa ttaatacagg
    10681 aatatgtaga tggtgaatgt atctatattc ctagaaaatc taataataaa aaagaatggg
    10741 gaagtaatac acctactcgt gaggagttgt caattagaaa tatgcagatt tacgaagact
    10801 atcaagttgg atatgatttg caatatttat cggaaaagta ttatttatct ttgaaaagta
    10861 tccaaagaat tgtattacaa gaaaaaagaa aagatatcta aggatgagcc agtatagttt
    10921 tataattata ctggctcatt ttcttttgat aaaagtgttt gaggtatagt ttcttttaag
    10981 tggatgatat aatttaagta atttcaataa cttatacagt agctaacaaa gctggttgta
    11041 aagtatctaa taagaagtag tagcttttta tatgtttgaa attaaaattt attaaaatta
    11101 ggaggtcttg ttttgaaaaa aatacttgat tatattccac cttttcataa ctttctatta
    11161 atttagtaat cttatagaaa ggaaattgca tttatgaaaa aaattacttg ggaggtggaa
    11221 tatatctccc agttacctgt aattaggtat tgtaaaaaat gtggaaaaaa gactgaatat
    11281 atttgttcag gtttatttag ggttaatgct caacataaat atttggatat atggttaata
    11341 tacaagtgtt caaattgtga ttcaacatgg aactcaacta tatattctcg tgttaatcca
    11401 aaaagcttaa atccagaaat actagaacaa ttccatacaa acgattataa tcttgttaag
    11461 caatatgcta tgaatatgga acttttacat agaaatggag cagaagttgg tattccagag
    11521 tataaaatag taggacaaga aattaatatt aatgacctta cacaagttca tattattagt
    11581 aaatatccat gtcagttaaa ggtatcaaca ttattacgtg agaaactagg tttatctagg
    11641 aaggtatttg acgaaatgtt ggattgtggg attattagaa gtaactcagg agttgattta
    11701 aagaaaggta aattaaatta tgaaattatg ttagatatag gcaaggagaa gcaaaagtct
    11761 tcaaattgat tagaataact aacttaatat attttcagga acatcattta aaaaaatagt
    11821 ggctttaaaa aatttaaagc cactattttt taatatgttt tatattaata tttaaaatat
    11881 agtgtgtatt cttcaaaatt atagcgataa ttaaaatatt actataaaaa ggaaggtgca
    11941 tactttttat ttaacaaaaa ctagatatta aaaacttttc gaggaatatt ttattaaaat
    12001 ccctaaatat atttaatata tattaaaaaa ttgtattata atgaaaatca aaatagattg
    12061 caaatccaaa aagtaatact taatgtccaa aaagctaaaa ttaaaatcta aatagctatt
    12121 tactacagaa gtatgttaaa atatgtaaaa tgaatagaaa tttatatatt tttattaaaa
    12181 atatataaga aaggatgttt aagaattgtg attaatatag gaatatgtga tgatgaacta
    12241 cattatcgaa taaaaataaa agatatatta agtgaaatat taagttcata tcctataaat
    12301 tataacatat atgaattttc agcaggagaa gagttactaa ataactatcc taaagattta
    12361 gacatattaa ttatggacat acaaatgaaa acaataaatg gtatggatac tgctaggaag
    12421 ataagagagt ttgatcataa gttagaaata atatttgtta cttcatttgt agagtttatg
    12481 caagaaggtt atgaggtaaa agcatacaga tatatactaa agccaataaa taaagaaaaa
    12541 atttctaaaa gtgtattacc ttgtataaat gagatgatga aaaagagaaa taattactta
    12601 actataaatg taaaaaatta tgtagaccga attaaaatag attctattac ttatatagaa
    12661 acagatagac cgaatatatt gatttataca catgatgata tgtatattac taaaatgagt
    12721 atatctaaaa tagaaaagat attaaatgaa tatggatttt ttagatgtca taatagctat
    12781 atagttaact taaaattagt agaatcaatg agtggaagta ctgttatagt agatggaaaa
    12841 agtattccaa taagtaaata tagggttaaa ggcttgaagc ttgcgataac taatatatta
    12901 ggggacatag tatgctaaca aatttaagtg taggattttg gaatattatt aatttagttt
    12961 caggtactat agattggatt atattttttt tagtaatcca cttagttgga aagaggaaaa
    13021 tatctaatat aagatatata tttacaacaa taattataat tatatttatg gcatacataa
    13081 atgtagttaa agtgtttcca aatattaaga ttcttctatg tatggtaata ggaatatcat
    13141 tttatataac atcatatgaa gataaaatct ataaaagtat aatagttagt ttattgtttt
    13201 ggctaggatt aatggttgct gaaggttctg cagtaggtct agtagtattt ataaataaat
    13261 taaataatat taacataaca caacatgaaa acttatttag aatacaagct attattatgt
    13321 caaaggtatt tttatttata ggttttatat tgtttaagta ttttaaatta tctttagatt
    13381 ttaaacctaa agatatgata ctaataggaa tacctatatt atctaatata gttagcttat
    13441 tacttatatt tgaatataat ttaagagtca atacaattac tacagaaaac ttatttatat
    13501 ttatatttgc aattttattg attttatcat caagtataat acttttaata gtaataggga
    13561 aaattgtaca agacgataaa ttaaatctag agtatgaact tataaatgaa agaataaaaa
    13621 caaactacag gaattatgaa aatataaacg aagttcatga tagattaaaa tatgtttatc
    13681 atgacttgaa aaaccatatg atttgtataa aaaattatga cactaaagaa gaaattatat
    13741 catatataaa taacttagag tttcagataa gtgaatttga acattttaaa aatactggaa
    13801 ataaaacatt agatttaatt ttagcagaaa aaataagtat ttgtaaaaag tatgatattc
    13861 aaattgaaga taacataaat atatctaaat taaactttat taaaaataat gatatatgtt
    13921 caatatttgc aaattcacta gataatgcaa ttgaagcatg tatggatata aataatgaga
    13981 tagaaaaaag aatagaagtt aaagctacat atataaataa atttgcaata ataaaattta
    14041 taaatactaa ggtaaatgat attaaactta ttgataaacg tattcaaact agtaaggatg
    14101 atgataagat acatggaata ggacttgcta gtattaaata tatagtaaat aaatatggtg
    14161 gtgaaacaat agttaattac tcagataatg agtttatatt aaaaataatg ataccaataa
    14221 aaagttagga gagtacaaaa tgaaaaaaat agcgttaaac ttacttaaaa acatctcagc
    14281 attaagtttt gggatagctg tattatcagc aaacagtgca agttcttggg tagcacatca
    14341 ggcaaaagag ccacaagctt tacaaaaact aaaaaaataa cattaataaa aggacctctt
    14401 cttattaaag gagaggtttt acatgttgag gaggaaatat gtttaaaaga ttatcatata
    14461 aatttgccaa tatattagtt aataatgaga ttgtagaaag tgaagatttt gaaatatata
    14521 gatatggatt tgaaactttg atttatttca tagttaatat tttggtagca ttatttatag
    14581 gaattatttt tgatagattt atacatacag ttatattttt aagttgttat tgcactttaa
    14641 gacagttcac aggaggttat catgctagga attataaaga gtgtacatta acatttgcag
    14701 ttatttattt gataactatt ttttcagcta ataatataga tataaataaa tataagtatt
    14761 tattagtatt attaatgatt attagtatac taacaattta caagttagct cctttagaac
    14821 atagaaacaa acctcttagt gaaagtgaga aaaagcatta tagaaagact gtgcaaaaaa
    14881 tactattcgt aataatatgt ttaattattt tatgtaaaat acttaatatt tttcagcaat
    14941 atgtgattta tgcaataatt tctatatatt ggattgctat attaatatat attggaatga
    15001 aggttaatag tgatcagtaa gattataaaa cattttctaa attaattaac ttcactaatt
    15061 aatattaact ttaaaaaaat aatgtcaagg taagtatttg aacttattaa atatagttga
    15121 atctattgta ggttgtatca aaagactggc taggaggggc ataaatatga gtagtattaa
    15181 ggctatatca gactttataa ccaaaataat tagaaaaaat attttatttg caataacaat
    15241 tattattata aattaccatc tcatacaacg atggacacat atatccaata gaggaatttc
    15301 atttaatgat tacttatcct tgagtgtata tacttgtatg acaattagta ttttaaccat
    15361 gttaaatgta attttttata tgagaaaaag ttatgatact gtaaaaatat tatgtagaaa
    15421 taattttgat tattttttag gtatttctgt tgctgtaact agaaacaact ttatattttg
    15481 ttgcatacca attatatatg ctatagcata ctcaagttta ataagaacat tgtcaattgt
    15541 aacgattaaa tcaactatat ttatattgat atttgaatgg tttttaccac ttactatttt
    15601 atctatagtg atatcattta tttcagttat tataaataat ttaatactaa agattacctt
    15661 atcatcagta tttttatatt taacatcaac taatatgttg gatagagtta ttgatattaa
    15721 agaaccattt tttaaagcat tatttaaatc acttaacata tttgaagatc aatcatatgc
    15781 ttactattct gattttgtag ggaaagttaa taattcagca tatgttatag ataagattat
    15841 acctacacta tttgcaatta ctttaataat agtagcatat ctgatattaa atgatattag
    15901 aaagaaacag attaaatcat taatagttat agtagtgtac agtattttat ttattggatt
    15961 aaattatata tcaatagata tgtcaacata tagagatgag gagcgtagtt atggagagta
    16021 tttaaagaaa tctaatatta gtgatattaa aataaaatcg catgatatga agattgactt
    16081 taaaagcaag tcaaatataa aagatagaat tgttttatta aacacatcaa atgaagagtt
    16141 aacaaatatt aatatgattt tagatgaaat atttgatata aaatatataa aaataaattc
    16201 ggaaaatata aaatttaaca aaaaaaagga tttaattgaa gttaatttaa aggaaagttt
    16261 agagcctaat gacgaggtga cattagatat agagtatgaa ggctatatta atgtgttgac
    16321 atcttggaat caaaataaat atatagctac taaagatgat attatgcttc caccaggttc
    16381 tttagcgtgg tacccaaaag taaatcaatc aagttgtatg gcttataaaa taaatttaag
    16441 ttctaaaaat aatgtctatt caaatttacc tttactgaat aagactagta attttttaaa
    16501 aaaagattat atatttgaag gaaagtcaaa tgatattgca atatatgcag gtgagtttaa
    16561 agaagaggta aaagatgata taaaaataat ctatccatca gatagtaatg taaatatgga
    16621 tatagaggaa aaaattaaat ttttaattga aacaagaaat gaaatattag gtgatagcaa
    16681 agcatctgta gatgctaaaa aatatgtaga tgaagtagat aatgttatgg aatcaataaa
    16741 aagaaaaaaa attaatatga tattagttgc tgacttacct agcaatgaat ttttatatga
    16801 agactcaaag ggagagaaaa ttagcataac tatttatcat tggctttcag gaaatatcct
    16861 tataatagat aaataataaa ggaggttaat atgataaaca agttagaaat aagaaatcta
    16921 aattttaatt acaaaaataa aaaagctttg gataatataa atttaaccct aatagatgga
    16981 gtagttgcat tattaggtcc taatggtgct ggaaaatcaa cattaatgag gttattagtg
    17041 actctttatg aaacttcaat tggagagata gaattaaata atataaagta tagcaaaaac
    17101 aatgagaaaa tcaaggcaaa tgtaggatat gtacctcaag attttgatat gtataataat
    17161 ataaatgggc aagaatactt agaatttgta gctaaaatga gaggtgtatc taagaatgat
    17221 ttaaagaaac atatacaaaa agtagtatca aaagttaatc tggacaagtt tataaataaa
    17281 aaaataggta catactcagg tggtgtaaag agacgactag gtatagcaca ggctttaata
    17341 ggtgattcta agttaatagt tatggacgaa cctacagttg gtcttgatcc agagcaaagg
    17401 aatgaattta gaagattgtt accagaaata agtaaaaatt ctatagtatt aatttcaact
    17461 cacatagtag aagacataca atttaactgt gacaaattaa ttatattaaa tcaaggcaaa
    17521 atattatatg atggtacaat taataagttt attgatatgg ttgaggtgta ttcggtagta
    17581 gtttctaatg atgaatttga atatttagaa cgtaatattg atattattga ttataggaga
    17641 gttaaagatg gtgtgcaagt aaagtatata ggtaaaaata cagagaaact taaagatagc
    17701 ttaaggatag atacaacttt acaagatgct tatataaact ttttacataa ggagttaaaa
    17761 taaatgtgta tattggagtt aataaaattt gagcttaaaa aaatagagct taaacatgtg
    17821 ttaatatttg taattatttc tatcttatgc atatttatta caggcatatt agattataat
    17881 gagttaatat tttataatga tgtacctata atggatacat attctatagc aggatggtta
    17941 ggaattataa agatatcaga tgtttttata ccaataatgt cagcaatcac aataatttat
    18001 gtgttttcaa aagattattc gggtaatata aatgagatac ttactttata taataaaaaa
    18061 aaatataatc aatttatagt atttagatgg tgtgttatat tagctatata tactgtatta
    18121 ttattaattt caattacaat agtatataca tttacgtcag tgaaaggttt taacatagac
    18181 aaaatagatt ttgtttatag tgtaaagcca atagatgtgt ttttaaagac atttcctaca
    18241 ttattatggt atacaacatt tccactaatg atattaacat taacaaaaaa tagatttaca
    18301 acaatagcaa tattaattac atatacattt gctgatatat tttttatttt acatatgtac
    18361 ccatttgtta gtatgattaa tgtaaatgga ttttatattc aaaagatgtt ttatatgcct
    18421 aacaatatag atgttagatt taatgaatta aagaattatt tctttataaa tagaggttct
    18481 ctagtagtta tttctataat aatagtttct tatatagcaa gaaaatcaac ggttttaaaa
    18541 aagagtaaat agtttaatat attttttata atgttatatt tatatagaat agtgacttta
    18601 aaaaatttaa agccactatt ttttaatatg ttttatctat ataactatta tttttctagg
    18661 ttttttaata tttcgtaata gtgtgttgtg gctatttcac ttgactcgcc ttttttacaa
    18721 aaagataatt tagatagttt tggaactaca cgttcaacga caattttatt acctatcatt
    18781 ttagattcaa gtatcttcaa tgcttctaaa aatctattat ccttttttgc acaattataa
    18841 aatgataaaa cgtagacata aaggaatagg ttataattac ggaaaggata ttcaacttgc
    18901 ataaataagg ttccaattcc atagtgacaa gggccgattg gttttttaat agtccaatgg
    18961 tcaagtagaa aatctacagc tttgtcaagt tttgattctt tatttagata attactaaaa
    19021 cggaatgcat ttaatatatt aagagttggt agtggatttg agtattcagt ttctggacct
    19081 cgaccaaaac taaatttatt gcaacgccac ccaccatcag tatactgtgt atctaaaaaa
    19141 tgttgaaacg ttttttgcat tctaatgtca gatgcatatc ccatatgaca gagtacgtta
    19201 gcagcatttg cagtatgaca agggtaaata cctccatttg gatatagttt gaaacgtcca
    19261 tcttctcgcc atgtgctaaa tatcaagtca gcacattctt taagtaaagt atcatcaggg
    19321 ttcacaccta attctaatag ataaagtaca ctatcaagag tagagaaagg agaacctttt
    19381 attaaacgct tatcaggtgt agtccaatag tctgcaccat tatcatacct gtgtgataat
    19441 attatttcaa tatctgataa agattgattt tctacattca ttttttactc catttcatat
    19501 tatattctat taaatctatc aaaaacttta ttataatatt atttatattt ataattgatt
    19561 tattttattt aaaattgcat ttatagctgt atcaggaaca actgctaaat cttctgcatt
    19621 gaagtaataa actatattag aatcaaataa tatatttgaa tttggaggaa attcatcatc
    19681 tccaaaccaa agtataaata ccatagatgt attacccata tatgtaaacc tataagctaa
    19741 atctcccatg tttacttttt cagctccaat tttctccata actaacttat atttgtctat
    19801 ttgagatgta aaaacttctg aaagcctata tagagtcctg tttaaaaaat ttggataata
    19861 aacattgcct cctggtattt ctttaaaagt tacatattta ttagttctag gaataccttt
    19921 agcattgatt aaatatctaa ctatcaagat tttaagctca aagttattgc aaaggctacc
    19981 attactattc caaacttctc cacttggata gtctataata tattgtctat caaaaaaatt
    20041 gagtgtgaat ctatttaaat cattattgaa aggtatatta cttaaattac taattgtatg
    20101 agggtctaat tctttgagtt tttttcgaac atagtcaaat ggaacattat aattattttt
    20161 tgttatcttt tcatccataa ttttttcctc caatttaaac aattaaaagt atatttataa
    20221 aaagttttct tattattaat ttt
//
LOCUS       DAMJZE010000061        19753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJZE010000061 DAMJZE010000000
VERSION     DAMJZE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.061"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            115..1308
                     /locus_tag="PC541_001905"
     CDS             115..1308
                     /locus_tag="PC541_001905"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2839371.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1514..2632
                     /locus_tag="PC541_001906"
     CDS             1514..2632
                     /locus_tag="PC541_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2839372.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2763..3008
                     /locus_tag="PC541_001907"
     CDS             2763..3008
                     /locus_tag="PC541_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2839373.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            2995..3741
                     /locus_tag="PC541_001908"
     CDS             2995..3741
                     /locus_tag="PC541_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2839374.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4026..4901
                     /gene="hslO"
                     /locus_tag="PC541_001909"
     CDS             4026..4901
                     /gene="hslO"
                     /locus_tag="PC541_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2839375.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5292..6092
                     /locus_tag="PC541_001910"
     CDS             5292..6092
                     /locus_tag="PC541_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2839376.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6620..8860
                     /locus_tag="PC541_001911"
     CDS             6620..8860
                     /locus_tag="PC541_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2839377.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9274..10809
                     /locus_tag="PC541_001912"
     CDS             9274..10809
                     /locus_tag="PC541_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2839378.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10825..11586
                     /locus_tag="PC541_001913"
     CDS             10825..11586
                     /locus_tag="PC541_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839379.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11924..12793
                     /locus_tag="PC541_001914"
     CDS             11924..12793
                     /locus_tag="PC541_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2839380.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13122..13373
                     /locus_tag="PC541_001915"
     CDS             13122..13373
                     /locus_tag="PC541_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2839381.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13485..14264
                     /locus_tag="PC541_001916"
     CDS             13485..14264
                     /locus_tag="PC541_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2839382.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14399..15049)
                     /locus_tag="PC541_001917"
     CDS             complement(14399..15049)
                     /locus_tag="PC541_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839383.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(15046..15549)
                     /locus_tag="PC541_001918"
     CDS             complement(15046..15549)
                     /locus_tag="PC541_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2839384.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15598..16113)
                     /locus_tag="PC541_001919"
     CDS             complement(15598..16113)
                     /locus_tag="PC541_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2839385.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16393..16920
                     /locus_tag="PC541_001920"
     CDS             16393..16920
                     /locus_tag="PC541_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2839386.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            16904..18307
                     /locus_tag="PC541_001921"
     CDS             16904..18307
                     /locus_tag="PC541_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2839387.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(18645..18956)
                     /locus_tag="PC541_001922"
     CDS             complement(18645..18956)
                     /locus_tag="PC541_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839388.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(19012..19605)
                     /locus_tag="PC541_001923"
     CDS             complement(19012..19605)
                     /locus_tag="PC541_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2839389.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         7515 a         2150 c         3506 g         6582 t
ORIGIN      
        1 aaaaataaat tttatgagaa tttaataact aataattaat atatttaggt tataataaat
       61 atgtgcgtaa aatctaacaa taagcaataa taccgtaaat ttaggaggaa taatatggat
      121 actttaaagg aactttttaa aataggaagt ggaccttcaa gttctcatac tatgggacca
      181 caaagagcag cagagagatt taagaatgaa aacccagatg cagaaagttt tagagcaata
      241 ttatatggta gtttggcagc tacagggaaa ggtcacttaa ctgactatat aattgaaaaa
      301 actatagcac ctaaaaaagt cgaaattgtt tgggaagaag atattataaa agattttcac
      361 ccaaatggga tgaagtttga agctttagat aaagataaaa atgtaacagc tgaatggact
      421 gtatattctg taggtggagg gactatagca gaagaaggtc aaagaaacag taaaagcaat
      481 agtatatatc acttagatac tatggatgag atagtaaaat ggtgcaaaga aaataataag
      541 actttggtag attttgtgtt ggaatgtgaa ccaaaagaca taaaagatta tataaaaact
      601 ataaaagatg ctatgagaaa atctattgat gatggactaa gtacagatga aataatacca
      661 ggcaagttat tattaaaaag aagagcaagt acattttata atgcatataa aaaagataag
      721 agcttctcaa cacttgtata tgcatatgca ctagcagctt cagagcaaaa cgcttctggt
      781 aatataatag ttacagcacc aacttgtggt tctgctggag taataccagg aatattcttt
      841 gcaatgcagg atttctataa ttatgatgac gaaaaaataa ttgaagcact acttgtagct
      901 ggaataatag gtaatataat aaaaactaac gcttcaatat ctggagcaga ggtagggtgt
      961 caaggtgaag ttggagccgc ttgttctatg gcagcagcag cagtagcata cttaaaaggt
     1021 ggaactatag accatattga atatgcggca gaaatagcac ttgaacatca tttaggaatg
     1081 acttgtgacc ctgtatatgg atatgttcaa attccatgta tagaaagaaa tgcaatggca
     1141 gctcaaagag cttatgatgc agctaattat gcattattaa ctgatggctc acattctgtc
     1201 tccttagacc aagttgttga aacaatgaaa gaaactggta tagatatgat ggataagtac
     1261 aaagaaacag ctaagggtgg acttgctaaa catttcttct cttgctaaaa ataaaatttt
     1321 ccaataataa ttcctctgtt ttgatttatg aaacagggga attattatta atggtgaatt
     1381 atttaataat gcaaaatatt tttatattca atttagcttt ttatttttaa ttagtataac
     1441 atgctatata atattttaat tataaagaaa tatagaaata ttttttaata aattagaaat
     1501 tggaggagat tacatgagag aggcatcata tttacaagaa caactttata aacataggaa
     1561 gtcattaaat cctattgcag agattgggag aaaagagtat aagacttcaa aatatataag
     1621 agattatctt gataaattag gaattgagta tgaagtttat ttagaaacag gtatagtagg
     1681 tgtaatagaa ggtaaaagac ctaaaaaaga tattgctttt agagctgaca tagatggtct
     1741 tcatagtgaa gaaggggtaa aacatttatg tggtcatgat ggacatgcta ctatattact
     1801 tggattaatt gagtatttaa atgataataa agaagactta aatgataata tagtatttat
     1861 attccaacca gcagaagaag gtcctggggg agcaaaagct ttaatagatg aaggagtgct
     1921 tagaaaatac aatattgatg aaatatatgg gcttcatata tatccagagt taccagaagg
     1981 ttatgtaggt acaagagagg gctattttat ggctcaaata ggagatattg acatagaaat
     2041 agtgtctaaa agtggacatg gggctatgcc acaaaatggt attgatggta tagtgatagc
     2101 ttcaaacttt gtaagctcac ttcaaactat agtgtctaga aatataagcc caatagacaa
     2161 tgcagtgttg actattggta gaattgaagg gggagctaga agaaatataa tagctgaaaa
     2221 tataaagatg gaaggtacta tgagatgttt cagcccagaa gtgtatgaga aaatgaagct
     2281 tagagttaga gaacttgcta gagggtttga attggcttat aactgtaagg ttaatataaa
     2341 tattatagat gattatattg ctgttaaaaa tgacaaggat ttgtatcaag agtttgtaga
     2401 ggctattgga agtgatactg ttgtagagtt agagccactt atgatatcag aagatttttc
     2461 ttattatcaa aaagaagttc caggattatt ttttatgttg ggttcaagaa acgaagaaaa
     2521 aggatttgta aatggtcttc ataatataaa ctttaatttt gatgaaaaga tatgtgtaaa
     2581 tgcattgaat gtttattcaa aattattaaa atacaaagaa gcaatagatt aggaagataa
     2641 gttataaaga ttaaaaagca taaataccta aatttaaaaa aagtgtatta ataaataccc
     2701 cttacatatt tgtataaatt tattttaatt gacaaaaata caaataagaa ggggtgattt
     2761 ttttgaaaga aaaagtacaa agagaaaaga agataactaa tggcaagaat actaaaatat
     2821 tgacaaatga tgatataatg aaatatgaga tagcctcaga actaggtctt atggataaag
     2881 ttggtgaacg tggatgggca ggacttacag ctaaggaagc aggaagaatt ggaggtatgc
     2941 ttacatcacg caaaaagagg cttaaaaaga ttgtggagga gcagaataaa aaggatgagt
     3001 caatatagtg attttgcgtt tgtatatgat gaacttatga atgaagttga ttataatggt
     3061 tgggttaaat atatagaaga tataatagat agtgaaggtg taaaagtaaa aaatatattg
     3121 gaattggcat gtggcacggg taatcttaca attccattga ctaaaaaaaa ttacgatata
     3181 gctggtatag atatatctga tgagatgctt agtgtagcaa gagaaaaggc agaaaaagaa
     3241 ggagtagaat tagttttact tcaacaagat atatcagagc ttgattttga aatatctgat
     3301 ttggactgtg tactttgtgc ttgtgatgga tttaattata taacttatga tgatgatttg
     3361 gaaaatgtat tttcaaagac atatgaatta ttaaagaaag atggaatatt catatttgat
     3421 ataagttctt tttataaatt agccaacata ttgggaaata acatgtatgg agaaaataga
     3481 gaagatatag cgtatatgtg gcaaaattac tttgatgaag aagagaatct aattgaaatg
     3541 gaacttacat tctttattaa agatgaatgt ggaaagtttg aaagatttga agagatacac
     3601 caacaaagag catatactga agatgagata gttgatatgc ttgaaaaatc tggatttaag
     3661 gatataaaat attatgggga ttttacattt gacaaaccta gtgaagaaag tcaaagaatc
     3721 ttctttgtat gtaaaaaata agatattctt gactatataa gataattatt aaaaataacc
     3781 atgatataaa aatatataaa atgcagtttt atagattaaa agtaaagtga tgatatttta
     3841 tgatttttat tttctaataa ttataaagtt ttatagataa aaattaaaat ataataacta
     3901 ttgcttagta aaattaaaat aaaatgggtt ttgcccaagg ttaagataga taagtattta
     3961 tagattaaaa ttaaggtagt aatataataa aaataagaca attaacggga catggaggaa
     4021 tattaatgag agattatgta ctaagagcga catcaggaaa tggtcaagtt agagcatttg
     4081 tcgcaactac tagaaataca gtagaagaag ctagaagatt acatgaaact acaaaagtag
     4141 caacagcagc acttggaaga acattaacag ccacatctat aatgggatta atgatgaaaa
     4201 atgatagtga taaattgact gtaataataa aaggtggagg tccaattgga actattatcg
     4261 ccacatcaga ctctaaggga atggtaaaag gatatgtagg aaatccacaa gtagaggttg
     4321 aagactatcc aaatggaaag ttaaatgtag cagcagcagt aggtacagaa ggtgttgtaa
     4381 aagttataaa agacttaggt cttagagaac catataatgg gacttatcca ttggtaagtg
     4441 gagaaattgc agaagacttt acttattatt ttgctgtatc agagcagact ccatcagtag
     4501 tagctttagg tgttcttaca aaagaagatg aagtagagtt tgctggagga tttatagttc
     4561 aattaatgcc agatgcagaa gaagagacta ttgctaagtt agaggaaaat gtagctaaat
     4621 taccttctat aactaacatg ttaaaagaag gaaaatcacc agaggatata ttaaatattg
     4681 ttttggatgg tttggaacct aaaatacttg atacctgtga agttgggttt atgtgtgaat
     4741 gttcaaaaga aagagtaaaa actgctttgg ttgcaattgg taaaaaatca ttggctcaaa
     4801 taatagaaga agataaaaaa gcagaagttg gttgccagtt ctgtaataag aagtatatgt
     4861 atagtgaaga agagttgctt gaaattttaa aggaaatgta gactataaaa atagttgtat
     4921 tgatataagt aatgttatag taatatagta aagtcgtatt gatatagtaa ttaaagtata
     4981 atttattaat aatagacata tttatattaa aaaataagtt taataaattt ttaagcttaa
     5041 taaattttaa gaattaagaa gctttctggc tgtaatgttt gcaatgttgc agttagaagg
     5101 cttttttatt ttattatagt tcgtgtcaaa gattgtatga aacaataaaa tattgtaaaa
     5161 taatttgtat atttagacct gattgacaca aaggtttata agatatttga gttgattata
     5221 cgaaattcta tgaagtagta accctgtgac tttaatcatg ggaggttcag aagaaggaga
     5281 aaattttaaa tatggaaagt aaaacgaagt acataggaat aagtatatta atattattga
     5341 tgatattttt aattgcaaag aattttaacg gaaaacttga aatacctatt cctgagagta
     5401 atagtagaaa atttaaatca gcagcagtgt tttatcctca acatcaagat gatgaggttt
     5461 tatggggagg aagtgccatt gtagatgcta taaaacagtg tggtgttgat aatgtatatg
     5521 tggtattagt atcagatggt tctggagtta acgttttcaa agctaacaca aaatttagaa
     5581 atttaactag gaagcaaaag gaagaactta gaaataacga gtttaaatca gctttgagag
     5641 agcttggagt gaaacctcaa aatgtgatta tacttgcaga tatagataaa aaggaaggca
     5701 cacattatga attaatggaa aagactatat tggattttga acataagttt aaaggcaatg
     5761 tgacccatat agcccatcac tataagtatg atgaccatat aatgcataga aagaatgggg
     5821 aagttttaaa gaaacttaaa cgtgaaggta agataaaaga tgacttgtat tttgttaagc
     5881 caaaatatgt aaagtatata ccaagaaatg aaagagctat ctataaagca atggattttg
     5941 atgactacaa taaggttaag agtgcttgtt atgaatataa ggttgtaaat tcaaagcgta
     6001 aaatgtatgg tataggatat atttcggcac atagttattt tgaacatctt ctaaatgacc
     6061 ctgaacttac gtctgtatta agtgaagatt aatttcttat aagtagtaag ttatgtttga
     6121 taagttgatt ggaattttct atagttttga aggtattgga aatttagttt tatttaaaga
     6181 tttaaagata gaggttttta gtgtttaaaa ttttaaagtt aagttgaata attgtataga
     6241 attatttgtg aaagaagaaa cagtgtaaaa catagtatct tatttatggt tttaaaatca
     6301 taggcaagat acttttttta tgtaactaat accattttga gattattaac ttaagaattt
     6361 tatataattt aacactcata tataatgatg atatttaggt agaatagatg taattacgct
     6421 taaataaaat aactgtattt tttataaaaa tccaaaccct acactatact accagataaa
     6481 atctacatgc tataatttga cctgtgttta taaaaaaagg agtaatctaa taaaatagta
     6541 tttcacttat gtataactaa caactattat tacgattttc tctaaataat ttaaagaaaa
     6601 ataatgcagg aggtaaaata tgaacaatga atttataatt ttaaaaggtt gcaaagagaa
     6661 caatttgaac aatatttcct taaaaatacc aaaaagaaaa ataaccatat tcacaggggt
     6721 gtcgggttct ggaaagtcat ctattgtatt tgagacaatt gcaaaagaat cacaacgcca
     6781 actaaatgaa aggtttagta catttgtaag aagtttttta cctaagtatg gagaggttaa
     6841 agctgattgt atagaaaatt tgtcaacacc aattatcata gaccaaagta gacttggtgg
     6901 aaattcaagg tcaactctag gtactattac agatgtaaat tcctttttaa gagctttata
     6961 ttcaaggttt ggtagtgagt atataggtaa tgccaatatg ttttctttta ataatattga
     7021 tggtatgtgc ccacactgtc atgggcttgg aaaaaagcta gttcctaata tgaaccaaat
     7081 tcttgatatg aataaatcat taaatgaagg tgctatattg ctttcagggt ttggagtggg
     7141 ttcttggcat tggaatatat tcgctgaatc aggatttttt gatattaata agaggatatg
     7201 tgattattca gaagtagaat tggaaaagtt tttgcatggt aaagcagaaa agattaagat
     7261 agaaaatgct ggtcaaacta acatgacata tgagggatta atggttaaat ttaatcgcct
     7321 atacttaagt agagagggag aaatatctga agctactaag aaaaaattga gtaaacttct
     7381 tatagaagat agatgtccta tatgtgatgg aagaagactt aatgaaagag tatataggtc
     7441 tttaattaat ggttataata tagcagattt aactagtatg caaattgatg aattggctga
     7501 agttattaaa agtatagatg agccagaagc acaaccttta attaaaggaa taatagaaaa
     7561 gttaaacagt atcatagata taggtcttgg atatttaaca ctagatagag agacatcttc
     7621 tctatcagga ggggaatctc agcgtataaa gatggttaag tatttaaata gtaatcttgt
     7681 tgatttaatg tatatttttg atgaaccaag tgtaggactt catccaaaag atgtgtataa
     7741 attaaataat cttttaaaaa aattgcgtga taaagggaat acaattatag ttgttgaaca
     7801 tgacccagat gttattaaga ttgcagacca tattgtagat gtaggaccaa aggcaggtaa
     7861 gtatggtgga gaagttgtgt atgaaggcag ttatgaaaat ctacttacaa gtggtacttt
     7921 gacagggaat gctttaagta aatttcttcc tattaaagaa agtgtaagag aacatagtgg
     7981 atatttagaa gttaaaaatt gtaataaaaa caatttaaaa aatgtatcta ttaagatacc
     8041 taaaggtgta ttgactttag ttactggagt agcaggagca ggtaaaagta cattgattaa
     8101 ggatgaattt ttaaagcaaa atcctagtgc tgtacttata gaccaaagcc ctgtttcagc
     8161 aaattcaagg tcgagtttgg caacatatag tggaattatg aacaacataa gaagcatatt
     8221 ttcaaaaaca aatggcgtaa atgcttcttt attcagttca aattcagaag gagcttgtga
     8281 taactgtaag ggttctggaa tagtagaaat gaatcttgct tttatggaga gtataaagag
     8341 tacctgcaat atttgtgaag gtaaaaaatt taaaaaagaa gttttagaat ataggtttca
     8401 agggaagaat ataattgaag ttcttgaaat gtctgtttta gaggcaattg agttttttaa
     8461 tttaaaacag attaaaacga aattacaatg tattgaaaaa atgggtattg gataccttac
     8521 tttaggacaa actcttgata ctttgtctgg aggagagtgt caaagattaa agcttgctag
     8581 tgaattaaat aaggaaagtt cagtgtatat attagatgaa ccaacaacag gtcttcatat
     8641 ggctgatatt gagaatttta taagtataat tgaagagatt gtagataatg gaaatacggt
     8701 cataataatt gaacacaata tagatgtaat taaaagagct gattggatta ttgagctggg
     8761 acctgaagga ggaacaaaag gcgggagagt aatatttgaa ggtattccaa aacaattatg
     8821 taacaataaa atatcattaa cagcaaaata tatagtataa taaataaaat ataataaaat
     8881 tataatgata ttttaataaa tataatgggt atataaaatg ataattaaac ttaacagtaa
     8941 taaataatgt gagaaatatt caaatttaat taaataaagt gtcagaaatt tgtttaaatt
     9001 ttagtatttt aacggttctg accttttttc ttatttttat ggagaatatc gaggatggag
     9061 tctctaatag ttactttttt gtgtctactt gcaagtttga ttaaaaaatg gctatacaca
     9121 cttgtgactt cagtcatggg aggttcagat tataatctta atatataatt tgtgaaataa
     9181 tttgatgacg atttgctgat gtataaacaa taaagattaa ctacattaaa atttaaatct
     9241 tgttaagtaa aacttataaa aggagagaga tggatgataa atgatattct aaccttatta
     9301 tctgaaagaa gtgatttttt tataaagcta tccatagaac atttgatgat ttctattatt
     9361 tctataataa ttgcgattgt tttgggtggt ataattggta ttattgtaag tgaatttcat
     9421 aattcttcta aacctatatt gggaactatc aattttctat acaccattcc atctatttct
     9481 atgctaggat ttctgatacc attttccgga attggaaata ctactgctgt tattgcactt
     9541 accatttatg cattattgcc aatggttaga aatacatata ctggaattac taatgtggat
     9601 aaaaatattc ttgaagcagc taggggaatg ggaagtacag attttcagat attgtataaa
     9661 ataaaacttc cgttagccct acctgttatt atgtctggta ttagaaatat ggtgattatg
     9721 acaattgcat tagctggtat cgcttcattt ataggtgctg gaggcctagg tgtagccata
     9781 tatagaggaa tcacgacaaa taacacaata atgacaatta ctggaagtat acttattgct
     9841 ctattagctt taatatttga ttttatttta gggctggtag aaaaattttt tcaacgaaaa
     9901 agaaaaaaga aaaataggaa aaaagttttt gccattctag gaagccttat tcttatagta
     9961 attatcttta cgtcttattt atttaatcct tcaaagactc aaactattca tattgctact
    10021 aaacctatga ctgaacaata tattcttggt gagatgttgg atattttaat tgaaaaaaac
    10081 acaggtctaa acgtggaaat tacacaaggt gtaggagggg gaacatctaa tatccagcca
    10141 gctatggaga atggagaatt tgacatttat ccagagtata caggtacagg ctggaatatg
    10201 gtacttaaga aaaatgaatt atatactgaa agtatgttta gtaaactaca gtcggaatac
    10261 aaaactaaac ttaaaatgca atggactggt atgtatggtt tttctaatag ctatgcatta
    10321 gcagttagaa aagaagttgc agataaatac aatttaaaaa atataacgga cttaaaagat
    10381 gtatcttcta aattgacatt tggagctgaa tatgactttt atgagagaga ggatggatat
    10441 actccattgt gtaatacata tggactgaac tttaaagata cagttgattt agatattgga
    10501 ttaaaatacc aagctatcaa tgaaggtaag attgacgtaa tgcctatatt tacaactgat
    10561 ggtcagtata gtgtttcaga tgttgtagtt ctaaaagatg ataaaaattt ctacccatct
    10621 tatcaatgtg gaaatgttgt tcgtttggaa gtattagagg aaaatccaga actatcaaag
    10681 gtatttaaga tggttggaga tattttgact gataaagaaa tggctaaaat gaattatgac
    10741 gtagaaaaca acaatttaga acctagagaa gttgctcgtg agtttctgaa taagaaaggt
    10801 cttttataaa ttggaggaaa tataatgaca cctattatac aatttaaaaa tattaaaaaa
    10861 caatataatg ataaaactat aatagataat cttaatttag atatagaaaa aggagagttt
    10921 ttgactgtaa ttggttcgtc tggttctggt aagacaacac tcttaaaaat gattaatggt
    10981 ctgattttgc cagatggagg aaatattttg ataaaccaaa cagatatcaa aaacgaggac
    11041 ttaattaaat taagaagaag aataggatat tgtgtacagg gaagtgttct ttttcctcat
    11101 atgactgttg aagaaaatat ttcatatgta ccaaatctcc taagtaaaaa gaataaatta
    11161 gaagtaaaaa gtgctgtaaa caagtggatg gaaatagtgg gattgccaaa tgatatgaaa
    11221 gtgcgttacc catcagaatt gtcaggtggt cagcaacaac gtgtagggat tgcaagagct
    11281 ttagcttctt ctccagaaat tttattaatg gatgaacctt ttggtgcagt tgatgagatt
    11341 acacgaaagc aattacaaaa agagataaaa gaaattcata aaaaaacagg agttacgatt
    11401 atctttatta cgcatgatat ttatgaagct ttgattttgg gaacaaagac tttggtatta
    11461 aatcatggag ttgttcaaca gtatgatact cctgagaata ttttaaatac tcctgctaac
    11521 caatttgtag accagttatt gaatataaga aaatctataa tagatgatga taatttaaaa
    11581 gaataaattt aaatttaaaa tcaaattgtt gtgataaaaa ctattgattt agagctaact
    11641 tcaagttgta tacttgaagt ggaaaagtta gattaaggta tagaagggca tttagcagtt
    11701 ggctgtagac gaggtaactg ctgaaggtgc tttcaataaa tggctagaac ctgtatctgt
    11761 tgaagattat aataaattga aataagaagc tcaatataca gagtagatag gcacttcaaa
    11821 tagaagtgtc ttttgtgtgc cataaaataa atcaaaatat tagcaaagaa tgtgaaaaac
    11881 aaaataaaat aatatgctat ctttaaaata ggagggatgc tatatggata acaatttgat
    11941 taccattgaa aaaactttag attatataga aaaacatctt ttagatgaga tgttagattt
    12001 aaatgtaatt tctaaagaga taggctattc aaaataccat ttacatagaa tgtttacatc
    12061 agttgtcgga tttagtgtac ataattatat acaaagacgt agacttactg aagcagcgag
    12121 aatgctaatt tttacagaaa aaccaattat tgagattgct ttaatctcgg ggtatgatac
    12181 acaacgctct ttttcaagag ggtttaaaaa aatgtttaaa cgtagtccta atttttttag
    12241 aagacatcaa gattttttac cactacaatt aaaatttgat attcataata gaaaaaaatt
    12301 acgtggagat atggttttag atgttaaaaa agttaattgt gcagatataa atattgttgg
    12361 ctattctggt aaagtaagaa aaggatttca tattattgga aaatgctgga gggatttgca
    12421 taaaaataaa gagatgatag aaaataaggt agatttaggt tttttaattg gagttagtga
    12481 ttatagcaat tttaaagaga ttgagaatac tcctacattt acatacattg caggtgctca
    12541 agtagctact tttggcaaat tacctaatgg aatggaaaaa ttcgtattac cgaaaagtaa
    12601 ttatattgtt tttactttta ttgggaaaaa tgaggatagt atgcaaaatg ttgtagaata
    12661 tatctatcaa gaatggttcc ctaattcaag ttgccagttt aatgaaaata atttatatga
    12721 ctttgtaaaa tatggagaaa aaactgatga gaatggtttg agtgaaattc agttttgggt
    12781 tccaatcatt taaaagaagg tattatgtat aaagtagatg gatttatata ggcaaagttt
    12841 tacagcagta tgtatttata aataatttat agtttgtagg aaagaaataa ttttactata
    12901 agattatgat aaaatacaca atactatgac aaaatagtgt taaataatag atttaatttt
    12961 gcataggaaa agttctataa tggtgtgtat tcataaataa tttataatct gtaggaaaga
    13021 aatagtttta ctataagatt atgataaaat acacaatact atgataaaat agtactaaat
    13081 aatagattta attttgcaaa ggataaggtg ttttaataaa aatgaatgta gaaaacgaat
    13141 tacttaaaaa cctagataga ttgcatacaa cagagttagg cgttgtaaga attaagaaaa
    13201 atctttcttt ggagacaaat gatgtagtga actggtgtaa aactaaaata gaatctccaa
    13261 atgccattat taatagaaag ggaaaaaact ggtatataag tgtatatgat tgtattataa
    13321 cagtaaacgc tcacagttat acaattatta cagctcataa agaaaagaaa tgaattgcaa
    13381 aatacaaagg tcaaaagcat tttccactgt ttgtagtacc tatgaagaag tatataaaat
    13441 aatattactt ttgaataata atttaaattt tggaggttga aaaaatgaat gatactgtaa
    13501 aagaaaaatt ggcagagtca ctaacttctg atactactga tattttacct tatttgcctt
    13561 atttattaca agatttttgg gaattaggaa gttcaccaga acaaatgtta caattattta
    13621 gaaagcatat accttttaat gaggatacaa aagttttgga tttagcttgt ggaaaaggtg
    13681 ctgtatctgt gaagcttgca aaagagttga atatacatat caaaggtatt gatttgatac
    13741 ctgaatttat taattatgct agaagaaagg ctattgaata taaggttgat gctttgtgtg
    13801 attttgaaat gaatgatgct aatagttctg taaaaattga gcatgattat gattgtgtga
    13861 tttttggagc tgtaggggat attttaggta attataaaga aactataagt aagctattac
    13921 ctacaataaa aagtaagggg ttcattttaa ttgatgatgc atatgtagta gataaatcta
    13981 caaatgaaca gcttaatttt gagagggtgt atcccactta caatgagtgg tgtatattgt
    14041 tcaaagaatt aggtctgaag ctaattgaat gtataccatg tttagaactt gaaaatgatg
    14101 ttgaagagga aatgaattgg attataactc gtgcaaatga gctaattaaa aaatatcctt
    14161 ctcaaaaaga tatgtttgaa caatatgtta ttagccaaaa aaatgaatat actgatttac
    14221 agcaggacct tgttggagca acttggattt tacaaaaaga ataagataaa tattagacta
    14281 gataatcaat gttatagtga attatcccat gtctagtgaa tataaataac ataaaaaacc
    14341 gtcaagatga atagtttatt cgtgcttgac ggtttttagt atttgttgaa gtaaaatttt
    14401 atttctttag tatatcactt ttagtcaatc catttgattt agcttgagta ttccattgat
    14461 gattccccat aagtttatat aggaatctag ggaagttagg tgaaataaaa atagtacttt
    14521 tgttggaatc atctatatgt atattatcac taagtatatt ttttgaaagt tcttttaatg
    14581 caattcctaa atctttttta ggtcctttac ccaaaggtac ggattctagt gaaccaaaca
    14641 tctccccagc tccaattcca ataccttgaa cccatgtaaa gttgattttt ttacaccaat
    14701 gctctatcat ttgaattgcc aaatgattat gttcaccttc attgaatcca cagttaacta
    14761 ttgcatacac tggaataggt tttttatttt gtgtttttaa aaatttttca catttctcta
    14821 aaaaatgaag taaatgagat ggaataccat ctacataaag aggagatgcg attattaatc
    14881 tatcacaatt aagtatggca ttaagagcgt caggatactt agatgtgcta tctactttaa
    14941 atgtaaaaac tttattatca ttttgaataa gttgttttaa ttcagataaa aaatactgtg
    15001 aattattttt atttactttt ggactaccat ttatcattac tatattcata tggattcacc
    15061 tcttatatct tgtatatttt catagaaaca aacagagtgt tcttttatat tgaaatttat
    15121 agcatttcca tttactaatt tcgtagcagt ttctttttca gcttctgtaa tacaatctcc
    15181 atagaatata gcttttaaat ttatctgttt atcatatctg cttttatgac gcatcatatt
    15241 atcttttatt acaaagtatg gaagaatata attaatgctt ttatcaagta cgtttttgac
    15301 aaaagggcta aaccctccgt aacagcatct gcttattata ataattccat cagagtcatt
    15361 tataagttta cccatgttgc tgtaataatc ttttacaaca cattcagaag gagttttaat
    15421 ccagcaacca aaacaaccaa tacatttgtg gatttctttt gtgtttggaa gtatttttat
    15481 attgtcatca agtttgggta ataacttttc taattctttg tcagttaaat catgtattaa
    15541 aagcctcata aagtccatct ccttttatta tgtatgttta cttctaaaaa ttatatctta
    15601 tagatttttc agtaaatctt tacaccaatt aataacaaaa ttataatagt caactccata
    15661 aatcattgtt gcatacatat atgtatattg gtcaacgcaa tgattttctt ctgcatcatt
    15721 tttttgttta cttaatttat ttagaagtaa ttctacttca gttattaaca tttttaagtt
    15781 tgtttcaatt atttctttgg gtagatactt gtaaaagaat atattaacaa ggtggtcttg
    15841 ctttgtttta gaaaaattta taggtttctt taaccattca agaaatactt ctttaccagt
    15901 attagttatg gaatataatt tttttaattt gctaccatct ataacttcgt gatagtgtat
    15961 atatcctttt gtttctaatc ttttaagtgc aggatagata cttccaaagc tggcgtcaaa
    16021 aaagtacgag gtactttctg acattatttg ttttaagtcg tatccactta attctttttc
    16081 cattaaaaat cctaaaataa tgtattctaa catattacct cctggtatca attagatata
    16141 tatcgattcg atatatttat tattacagat aataaatctt acgtcaagat gctttttaag
    16201 ataagctaaa aagaattagg agttattgat aaatatgcaa aaaaattaat ataaaaggta
    16261 attaaatatc aatgaagatt taaaaatagt taaatcaaag taagagtcta atatataaaa
    16321 tttatagtaa ctttatggtt taaatataaa cttgcgtgct attgacaaag tataaaatta
    16381 gcagtaaaat aattgcatga gcaaatagat tattgcgaac gcaaaaaaat aaggacaata
    16441 gatattaaag gagagaatat gagagagcta ttaaaatggg tttccattat aaatagatac
    16501 aatacaaatt atattgacag gaaattggcg gatattggaa tcaataatag ccaatatttt
    16561 tatgtattac acatatgtaa taatccaggt attacacagg acactatatt tgagaaaatt
    16621 cttgtgaatc ctagtaatat aacaagagct ttggcaaatt tagaaaaaga aggctttata
    16681 acaagagaac caggtgtaaa ggataaaaga acatggcatt tatatcctac agaaaaatct
    16741 ttaggatgtt atgatgaaat actaaaaata acaaatgggt atgttgagga gttgttgagt
    16801 tcatttgaga aagaagagca agagttgttt atgtcaatgt taaagaaagt tgcatttttg
    16861 gctattgata tgaatgaaga agcaaaattg gaggcgaagg aaaatggaag aaaaatataa
    16921 ggagccattg gaatatgaaa aggtttcaaa gttattaact aagtacgcaa ttcctagtat
    16981 tatagcaatg ttagtaagtg ctttatataa cattgttgac caaatattta ttgggcaagg
    17041 tgttggtgta ttgggtaatg ctgctacaaa tgtcactttt ccactaacaa caatatgtac
    17101 tgcaattgca ttattgttag gaattggagg agcgtctaag tgttcattgg aattaggtgc
    17161 taaaaattca gaaaaagcta caaaagcagc aggaaattct atatgcttga tggctgtttt
    17221 tggaataagt ttatttatag tagtatcaat atttttaaca ccactattaa aattctttgg
    17281 ctctacatca gaaatactac cttatgcaca cacatataca aaaattacat caattggatt
    17341 accatttctt ataatgtcca ctggaatgag taagttgata cttgcagatg ggagaccaaa
    17401 agcttctatg ttttgtatgt taataggagc agttattaat acaattttga atccattatt
    17461 catttttgtt tttaatatgg gaattgctgg gtctgcttta gcaactgtta ttgggcaact
    17521 tatatctttt ggaataagtg ttcattatat aaagaatttt caacatatta aattacataa
    17581 gaaaagtttt aaattagata gtataattta taaacatata tttgcgctag gttcaagctc
    17641 atgttttaat caattagcaa tgactgtaac acagatagtt atgaataata cattggcata
    17701 ttatggggct aggtcagttt atggaagtga gattccactt gcttgtgtcg gtatcattat
    17761 taaagtaaat atgattttta tatctataat tattggtata tcacaaggta tgcaaccaat
    17821 tatagggtat aattatgggg ctgaaaagta taatcgtgta aaagaagcat ataagtttgc
    17881 agtgggaagt gcaactatta tatctgttat tgcattctta tgtttccaat tcttcccaag
    17941 acaaattacg tctatatttg gtaacggaaa tgagacttat tttatatttg cagaacatta
    18001 ttttagaact ttcttgttct taacatttat aaatggtgtt caaatagtat catcaacttt
    18061 ctttacatca attgggaaat cagtgttggg actttttact tctttgactc gtcaaatttt
    18121 atttttaatt ccattgatag taattttacc aatgtttatg ggaattgatg gagtaatgtt
    18181 tgcaggacca atagcagatg cagcagcggc aatggtatgt atatactttg cagttcatga
    18241 aatgaaatca ttgactataa agcaaaataa tttgatgagt aaccataata agattgcaga
    18301 ggtataagac aatatttaag aagtatagaa aattaattta aaaaatataa taaattaatt
    18361 taggaagtgt agaaaattaa tctaaagaag tataataaac taatttagga agtatagaaa
    18421 attaatttaa agaagtataa taaactaatt taagaaatat agaaaattaa tttaaagaaa
    18481 tacaatacat taatttttaa gtaacattaa attaatgtaa gtaaaataat gttcatatga
    18541 atatttaaag gtgcattttc tgaagctaag tatatgttgt agagtgttat gtctctctat
    18601 cctgtgaagt ttaatagctc aagaaaatgc actatttatg atgattattt tgttttattt
    18661 ccaattttgg aaataactat tctaattaaa tctaaaatta taccataaat aagaaaagaa
    18721 agtatagatg ctatgtacca ataaatcgaa attgaccctg taattgttct acatgggatt
    18781 tgcatacctg aatccataaa aacaaaaaaa ctatcaactc tggaaataat agacaaaata
    18841 ggatttaaac ctattagaac aatacttttt gagtcttgac caatttgatg catataaatc
    18901 tcaaataatg caattaaaaa aaaccaagat gtaaaagatt taataatttt tttcatcaaa
    18961 atacctcaca taatataaat tctgtttgca ctatttgctt actacaacta tttatctcca
    19021 ttcagtcaca tcacttaatt gtaatctagg tcttcttgat ggttcttcat caggataacc
    19081 taaagatatt gctgccacta attgactatc tgtatttaac cattcacata attcacgata
    19141 tgcaaagtat acatcacaaa tccaaagact acctagacct aattccacag ctgtaagcga
    19201 catattctga attgcagctc caattgattg catatttgcc atttcataga atcgttcttc
    19261 aggggatagc ttttctaaag gtgatttacc taatatattt aaaataaaaa ttgttactgg
    19321 agcttgtttc atgatttcaa ctgtgtaatt tgcaccagcg atatgttgac gacttcctgg
    19381 aagaagccca ttatcattaa tttcattttg aatacctttg ctcatggctt taagcataga
    19441 ttctttttcc ttttcagtga tgacaacaaa acgccatggt tgcctgtttt tagatgaagg
    19501 tgcatttatt ccagcttcaa ttattttctc tatagtttca tgtgaaatgc tttgattttt
    19561 atattttctg atacttcgtc tttttgatat agaatcactt atcattttaa gtaacctccg
    19621 tttatttttt ggaaaaatct atttaatttt tattttctat tttaattatt ttagcattaa
    19681 tcagaaaaaa agtcattgat tttagtatac aattattaat gattatatta agaattgtat
    19741 caaaaagtat gtg
//
LOCUS       DAMJZE010000062        19367 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJZE010000062 DAMJZE010000000
VERSION     DAMJZE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.062"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..1013)
                     /locus_tag="PC541_001924"
     CDS             complement(<1..1013)
                     /locus_tag="PC541_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2839390.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNV"
     gene            complement(1061..1921)
                     /locus_tag="PC541_001925"
     CDS             complement(1061..1921)
                     /locus_tag="PC541_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2839391.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2190..2399
                     /locus_tag="PC541_001926"
     CDS             2190..2399
                     /locus_tag="PC541_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2839392.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2399..2665
                     /locus_tag="PC541_001927"
     CDS             2399..2665
                     /locus_tag="PC541_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2839393.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2694..3266
                     /locus_tag="PC541_001928"
     CDS             2694..3266
                     /locus_tag="PC541_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2839394.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3501..4691)
                     /locus_tag="PC541_001929"
     CDS             complement(3501..4691)
                     /locus_tag="PC541_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2839395.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4951..5382)
                     /gene="dut"
                     /locus_tag="PC541_001930"
     CDS             complement(4951..5382)
                     /gene="dut"
                     /locus_tag="PC541_001930"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2839396.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5495..7723
                     /locus_tag="PC541_001931"
     CDS             5495..7723
                     /locus_tag="PC541_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2839397.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7834..8319)
                     /locus_tag="PC541_001932"
     CDS             complement(7834..8319)
                     /locus_tag="PC541_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2839398.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8571..9794
                     /locus_tag="PC541_001933"
     CDS             8571..9794
                     /locus_tag="PC541_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2839399.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9798..12932
                     /locus_tag="PC541_001934"
     CDS             9798..12932
                     /locus_tag="PC541_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2839400.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12966..14264
                     /locus_tag="PC541_001935"
     CDS             12966..14264
                     /locus_tag="PC541_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2839401.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14553..14798)
                     /locus_tag="PC541_001936"
     CDS             complement(14553..14798)
                     /locus_tag="PC541_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2839402.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(15012..17639)
                     /locus_tag="PC541_001937"
     CDS             complement(15012..17639)
                     /locus_tag="PC541_001937"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2839403.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17700..18542)
                     /locus_tag="PC541_001938"
     CDS             complement(17700..18542)
                     /locus_tag="PC541_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2839404.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18549..19187)
                     /locus_tag="PC541_001939"
     CDS             complement(18549..19187)
                     /locus_tag="PC541_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839405.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         6750 a         3086 c         2681 g         6850 t
ORIGIN      
        1 acatttaggt tattacattt atttatatat attttttcat tatctaagtc ttcatcaaac
       61 atttttatta tattttttat ctcatacttg tactcatctt cattcattct ataaagcatt
      121 aaatcttcta gtacatcttt catgtcgaag tacacatctt ctaataaata aaaatagcct
      181 tcctctatat ctttcaattt atctatagta tccatttttt tacttatatc tttcataatt
      241 tctaaatttt gtatatttac atctatgatt gttaataagt acattctcac agtttttatt
      301 atttcaacag aaaaatctac aatattataa tttccttctt ttttatataa tgactcctta
      361 aatttgttaa aacaattatc taaattagaa tttatatcgt taaatggtgg tcttattatg
      421 tcacgtatta ttaaaagatt ttgtatatct acattatttg tgattgcctc atccaaaata
      481 aataaattat atgcatcatt taatgggtca tgggcatctc cactaaactc taagttaaca
      541 aattcaagta aatttttcaa tgatatatag ttcatacatc tcataccaca atttatatat
      601 ttttctttta tatctacaaa taaatctcta atgttattta caaaccttgg atggttattt
      661 tttttagcac ttctcatatc tgtgttattg aaacatgtca aatccaaatt accaaaagta
      721 tatattcctt taatatcaga aaatatgcca agccattttt taaactttct catgacctcc
      781 tcataacttg gtgcactctt caattcttca gaagatatat gtgttaattc ttgtatatga
      841 ggatacaact cctcattact tactggtctt attaaagatt taaatttatc aatatttttt
      901 gttttttcat catacacaac tgctccaatt gcaattatat cagaattaaa tactgctctt
      961 tttgatttag aatttggcat attcatttca aaatctatat atactctttt caatataaac
     1021 accctttcca attgtttata gtgattcttt ttaactattt ttattcaaaa ttttctaaga
     1081 aagaatgttt ctccccatct ttttgccaac caagtactga atcaagattt acattttctg
     1141 ccgccttaaa tatagatttt tctacatttt taaaatttga ttctaaactc tttattaagt
     1201 cttttatctc atatctttta ttacctgttt ttttagcagc aaccatacat gcacaattta
     1261 atggccatat accactattt tgaataaatc ttataatatg ttcttctctt atataataaa
     1321 gtggtcttat aatttttata ccttcaaaat tagtagaatt aagtttagga agcattgttt
     1381 taaaatttcc agcacacaat aagttaagca ttgttgtctc tattacatcg tcataatgat
     1441 gacctaatgc aagtttgttg caccctagtt cttctgcctt tgaatataat gctcctctcc
     1501 tcattcttgc acacatatag catggataat cttttgctat ttcatctgct atctcaaata
     1561 tccttgaatc aaataaatgt attggtatat ttagatactc acaattatct ataagtaatt
     1621 gtcttatatt tgcatgatat ccagggtcca tagcaataaa ttcaacatca aaattaactt
     1681 gtccatgttt ttttaactct tgaaacattt tagccataag aatactgtct tttccaccag
     1741 aaatcgctac agctatctta tcaccttctt caaccaattt ataatctctt attgccttca
     1801 taaacttaga ccataaattt tttctatatt tttttattat acttttttct atatctctta
     1861 ctggaagtct ttcctcaaaa ggaactataa tttcacagcc ttttcctgat agttcactca
     1921 tctaatcacc tcgtatattt atgttaataa tattttacaa aaaagtctac cttcatccac
     1981 aaaatactat aatttttact ttaattttac taaataattt atagtttaca ataacacata
     2041 aacttataat aacataaata acctctttct tttattatct tttttatttt tctcatttaa
     2101 tattttatta ttagtaacga tttttaaatg attgaatatt atacactaga tagagttacc
     2161 tatctattta caacaaggag gttttcatct tggaaaataa gactagagat atcaaaaaaa
     2221 attgtggtcc atctttggct agaccataca ttaataatca aattttcaca gaaatgtact
     2281 gcctttctga tgcattaaaa tttggaacta tatttccaga actaaactta ctagagtctg
     2341 aaatgtacaa caaagaatta tatgctaagc caaaaaaata tagaggaggt agaagataat
     2401 gaaagactca tctagagctg aacttatgag aaaagttcag gaaacatctt ttgcatgtgt
     2461 tgatatgaac ttatatttag acaatcaccc tgatgataaa aatgctatca acacttataa
     2521 ttcattatgt aatcaatttg cgcaagctag atatgcttac gaaaataaat acggtccttt
     2581 aactaacttt gggtatgctc caagtagatg cccttggcaa tgggctgacc aaccttggcc
     2641 ttgggacaga gaatttaatt attagacatc aagtattgga ggattgaaaa ttcatgtgga
     2701 tatatcagaa aactatacaa cacccagtta atataaaaac ttgtgaccct agaatggcta
     2761 aatttcttat aactcaattt ggtgggccaa atggagaact tgctgcatct ttaagatatt
     2821 taagccaaag atatacaatg cctactggta atatgcgtgc acttttaaca gatattggta
     2881 cagaagaact agctcacgtt gagcttatat gtactatggt ttatcagtta acttctgatg
     2941 caagcccaga agagttaaaa gctgcaggtc ttggttcaaa ctatgctcaa aatggatatg
     3001 gaatttatcc aacagattca aatggtgttc catttgatgt aagacctata gcagttatgt
     3061 caaatcccgt aaccgattta catgaggata tggcagctga acaaaaagca cttgcaactt
     3121 attatcaact tataaaccta acagatgacg ttgatgttat agatgtatta aaattcttgg
     3181 gtcaaagaga aataattcac tatcaaagat ttggtgaagc tttaatggat gcttacgagt
     3241 tagaagaatc tcaaaaaatg ttctaaaatt tttatttgta taactattat tatttgataa
     3301 tttataattt actgttgtgt taaaatttat aaaaaatata aatataattt attttctata
     3361 aacagagaaa ataaataaaa agcaggtatc aatcaaatag taacatgtat tatactatac
     3421 attactatca atattgtacc tgctttcata ttataagcaa taaagaaaaa tctatttatt
     3481 aggttttata ataaaaacaa ttataaaact cttcttgcat ttacatatct acttgaatag
     3541 tagctactat ttatattaga tattgttact ttttttgatg aacctgaaga tgcatgtatc
     3601 atatctccat ttcctatgta tattccaaca tgacttacag aaccattatt tgaaccttgt
     3661 gtatcaaaga atactaaatc tccacttcta aggtctcctc tatttacata tgttccatac
     3721 ttactttggt ctctagatac tcttggtata ctaactccta cagatttttt cataacgtac
     3781 tgtgtgaatc cagaacagtc aaaactattt ggaccttctg ctccccatac atatggtttt
     3841 ccaagtagtg tctttgcaaa acttattaca ctatcagctc cttgacttgg agaattagtc
     3901 gaataagatg gaattgaagt atcaacagaa cctttttcag caaggtattg acttgacaca
     3961 tatccagttt gattgccata acttactttt gtccatcctc cactttcaga aatacttcca
     4021 acctcagtac cataacttaa tgtagctatt ttagaatgac ttgtacctgg tcctgttcta
     4081 acgttcaatg actttgctgt tacaaccttt gtacccttta caaggtcatt tgaactgttt
     4141 tcagcatttt catttgtaga gtcatttaca ctagttccta aatatttact tgaaacataa
     4201 cctactctac cattagaact tatttttgac catccatttg actcagatat gtatccgact
     4261 tcagtcccat atcctagtgt tgatatttta gaactacttg tacttggtcc tgttctaaaa
     4321 tttaatcctt ttgctgttac aacttttgtc gacttaactg aagtatcact actttcattg
     4381 ctacttccaa ggctatttat tgctacataa tttccatgca cataacctgt gtttccatta
     4441 tatttaactt ttacccaaga accttctttt cctacgtact caactttatc acctttgtaa
     4501 agtttcccca ttgattcatg gtttgtacct ggtccttttc taaaatttac cgcattccct
     4561 gttattgttc tgctttctat actcacactc tctacttcag actcattagc atatgctaaa
     4621 ttccccatag taggcattat tatcgcagtt gccattatac tggctgctat tctcttttga
     4681 ttaaaattca taactttcca ctcatctcct cttttctact atctttattt cacttcaaaa
     4741 tataaaagaa ataattgaca ctattaaaat atcaattatt tcttttatat taatattatt
     4801 acaaatttgt aactatttgt ctatagtaat atttgcttac cataaattac catgtataaa
     4861 ttttaatctt taatagcctg taatttagta atatagccat cttttacctt ttttaaaaat
     4921 ggctatattt tttaactttt ttgtaacttt ttaaaatcca gttgagccaa acccaccttt
     4981 tcctctttcg gagtcactta attcttttac ttcttctatg ttaatatcat agataggttt
     5041 tactatcatt tgagcaattt tcatatgctt ttcaacctta aaatcatttt taccatgatt
     5101 tattaatata attttaagct ctcctctgta tccttcgtca atagttcctg gtgtatttaa
     5161 tacagtaaca gaatgcttta aggcaagccc acttctaggt cttacctgcg cttctgtcat
     5221 agtaggaagc tctatacata tcccagtttt gataagttta gtctctccag gaggtattac
     5281 aacctcttct atagaataca aatccatacc agcatcacct ttgtgagcaa aattaggtat
     5341 tatagcatca tcatttaatt tttttacttt taaattgtac atcttatatc tcctttcaac
     5401 tttctataaa tattataact taaatttcga cctcttgaag ttttttattt ttttgtggta
     5461 atataattat tagtgtttta aaggaggatt ttttatgaaa cttgtacttg ctgagaaacc
     5521 atctgtggca aaaactatag catcattttt aggtgcaaag accagacaag atggttactt
     5581 tgaaggaaat gattatataa ttacttatgc agttggacac ttagtatcat tatatgatat
     5641 gaaggattat gataaagata aatactctgg ctcttggaaa atgaacaatt tcccatttgt
     5701 tcctgaggat aagtttaaat ttaagattga tagttctaaa actaaacaat tcaacactgt
     5761 aaaaaagctt ttgaatagaa aagatgtaga atatgtaata aatgccactg acaatgaccg
     5821 agaaggagaa cttatagcat ttttaatatt tttactggca aaaaataaaa aaccagtaaa
     5881 aagaatcttg gtaaatgaat ggacaccaga agatattaca agaggtataa aaaacctaaa
     5941 agatgaagat gaaatgagaa atcttcaagc tgctggatat actaggctta ttactgactg
     6001 gttgattgga ataaatttca cttctgttgc tacattgaaa tatggaaatg ggaaactatt
     6061 aaacataggt agggtaattc ttccaacagt taaattagtc tatgatagag atatggaaat
     6121 actaaacttt gttcctaaaa cctattatga aatagaaggg cattttaaag ctgaagctgg
     6181 tgaatataaa ggtaaatatg ttaagggtaa agaaagtaaa tttgatacac ttgaagatgc
     6241 aaataaaatt atagcttcaa ttacatctga aactggtaaa atactagata aaaaagttac
     6301 tatgtcaaag gaatatgcac caaaactttt tagtttgaca tcacttcaag gatatataac
     6361 ttctaagtat tccaatttta catctgataa ggttttaaat gtctgtcaat ctctctatga
     6421 gggtaatggt aagggtggat acataactta ccctagaact gattccattt tcttagaaga
     6481 aagtttggct tcaaaagctt ctcaaacttt agataaatta aaggttggtc ttgaatatga
     6541 aaataaaatt aaattttcta aaactaaaag agtttttgat tcttctaaag tagactccca
     6601 tagtgctata attccaactt atataattcc aaaaagttta tctaaggatg aacaattagt
     6661 ttatgatgcc ataaaagata gatttgtagc aaactttatg cctcctgctg aatatgagaa
     6721 tacagaaatt aagactgaag tagataattg tactttctta acaaaaggta aggtgcttaa
     6781 atcaaaagga taccttgagg tatataataa ggaagaaaaa aacgatttac ttccacttgt
     6841 aaataaagat gatgttgtag atgtgttaga aataaaacca ttaactaagc aaactactcc
     6901 acctaaacca tatactgagg acacactttt aaaggctatg aaaaattgtg gtaaaaatgt
     6961 gcctgaagaa gatacaacag tgctatctgg atattcgatt ggaacatcag ccacacgtgc
     7021 tgatgtgtta aagaagataa gtcaagtggg ctatgtaaag aaaaaaggta aatcttattc
     7081 tataacagac cttgggaaaa atttagttga aatttttcct gttaaagact tatttgatgt
     7141 agattatact ggaaaacttg aaaaaagttt aagcgatatt caaaaaggaa agtatacaag
     7201 aaaagaatat cttactaata tcattagttt tatatatcaa aatgtcaata ttataaaaca
     7261 agatgcttca aaaaatataa acacagaagc atatactttc gattcaaaaa ctaaaaaatt
     7321 tactaaaaca aaagaaaaaa aacagaaaaa agatgtaact aatactgaaa aagaagttgc
     7381 tatatcaaag gattctaata acaaaaaaga agttaaaaat ggtgatgaaa atcaatctct
     7441 tggcaaatgc cccatttgtc aaggtgatgt actagaattt gacaaaggct ttgcttgtaa
     7501 aaatcataaa gaatgtaaat ttgttatttg gaaaaatgat aagtatttaa gtctttataa
     7561 aaagaaagtt aacaaaacaa tggtcaaaaa tattttaaaa aaaggtgaaa ctaaagtaaa
     7621 atctttaact gctaaaaacg gtaataaatt tgatgctaca ttaaaatatg ataaaaatcc
     7681 agatactggc tatttcaatt ggaaaataga gtttgacaac taaatattca taagaaaaga
     7741 aatagcagta gtataataat ctatattata ttactgctat ttcttttctt atgaagtttt
     7801 aactttttta atagtgactt ttacaatcac actctaacct aatttcttta ctatttctga
     7861 taactttaat gtatgttcat ctaataaagt tagttcctct tcagaaagat tagataattt
     7921 ttttactaga gattctttga gaatagcttt tactgcatca aataatttaa atgctttatc
     7981 tgttaattca accctaagaa tccttctatc ttttgggtca ggaaatttat tgactaaacc
     8041 atgttccact aatctgtcaa taatcggagt catatttgat tttaaaattc ctaaattaga
     8101 agctatttga gaaattgatg atgaatttga tgtcataaga taaagaatta ccttagcatg
     8161 tgatggagcc attggacatt cttcaaaata agctttcatt atatcagagt tattttgaaa
     8221 cttttttaac aaatcgcttt ctttaaatat attattttca agcacaaata aaaaatctaa
     8281 aaaagagtcc gctattttaa gtatattgtt gttatccata tttgtaacat cccttcaata
     8341 gataatataa taaaacattg ttgtttaata tagtataaat tataaactat taataaattt
     8401 aaaacaagaa aaagttattg actcataata attataaatt tataatcaaa ttagagaatt
     8461 ataaattgcc aataaaggga agttttttaa ttagtctaaa actaaactta aattataaga
     8521 cactttaaat tctacaacaa gctagtgtgc aatatataat aggaggagat atgaaaagat
     8581 atttaatagt tttcttaatg tgtagttcac tgtttttagt tggatgtgga aaaacagaag
     8641 aatctgaacc tgagaaacca atagcagttt ctgttcaaaa agcaataggt ggagaaattg
     8701 aaaacacaaa ttctttctca ggaactacaa aggttaaaga tgaaacagct gttactgcac
     8761 agactgttgg cacagttcaa gaagtgtatg taaaacttgg gcaaaatgta agaaaaggag
     8821 atgaattatt gagtataagc agtccagagc tagaaaatag tgtaaagcaa agtaaagcct
     8881 cactagattt agcaaaagca agttattcaa gtgccactgg tggttcatta gaggcacaag
     8941 taaatcaggc aaaaacagct cttgacaatg caaagattca atatgaggag tctcaaagaa
     9001 attatgacaa taataagatt ctttatgaac aggaagtcat tagtttagac caatttaaaa
     9061 aaatagaatt ttctttagag caaacaaaac aacagttaga ttctgctcaa agagcatacg
     9121 atacagcaac ttcaaaaagt atacctcaag ctaaagcact tgctaaaaaa cagcttgacc
     9181 aagctcaagt atcatataat ttagcaatga gtaatttaga caaattaaca ttaacatctc
     9241 cagtagatgg tacaataaca gctaaaaact ttgattcaaa ggaaatgata acacaatcac
     9301 aaccagcttt tattatttct aatcctagta tactagaagt tgatttaaat gtagcagaat
     9361 cagatatagg taaatttaag aaagatggaa atgtagatgt tataattgaa gaccaaagga
     9421 ttttaggaaa aatagattat gtaccttcag tagttgaccc tcagacatct ttatatcctg
     9481 ttaaagtact tgtaaataat acaaacaaca aatttaaggc aggtatgtct gctcaagtaa
     9541 acttaagtgt tgaaaaagaa aatggtgctg ttacagtacc taaaaaagca atatttgaag
     9601 aaaatggtaa aaaatatgta tacatagcaa caaaagacaa tatagcaaag aaacacttgg
     9661 ttcaaactgg aattgtaaca gaggataaaa tagaaattaa aagtggtgta agtgataaag
     9721 atacagtgat tattggaggg ataagtctta tttctgacgg aactaagata tttcctgtag
     9781 aaaaggagaa gtagactatg aatttaacac agacttccgt taaacgtcct ctcacaatca
     9841 taatggtctt tttagtatta atagtttttg gtggtattgg atataaaaaa atgtcaataa
     9901 atctaatgcc tgatatagaa ataccagttg taatggtcat gactacttgg acaggtgctg
     9961 gtcctcaaga cgttgatgaa caagttagtc aaaaagttga tgaaagttta tctgccgttt
    10021 caaatgtaaa atctactata tcaagttcac aagaaagtgt ttctatggta gttgctcagt
    10081 ttgaatttgg aactaatttg gatgaaataa tgaatgatgt taggtctaaa gttgatgcat
    10141 tacagaccag cctcccagat gatgcagcta aaccaacagt tttaaagctg gatatgaatg
    10201 cacaggccat aggtcaactt gtcatatctg gaggaaatga aaatagttca caagccttaa
    10261 gaaaatacgc tgaagatgta attcagccta aaatagaatc catagatggt gttacttcag
    10321 cagaccttaa aggtggaaaa aaagcacagg ttaatgtaat agctgaccca gctgttcttt
    10381 caaattatgg ggttagttta tccactataa aaggtgtctt atcctctagt aataaaacat
    10441 tcccttatgg ttcaattact caaggtgagg ataaaatagt tttaagagcg attgataaat
    10501 tagaatccct agatgatata aaacagattc aaattcctgt aaaaggagga aatacagtta
    10561 gattagataa tctttgtagt gtagattatg gatatgcaga caaagatagt atttatagat
    10621 acaatggaaa agacagtttg gtaatagata tttctaaaca acaagatgcc aacactgtaa
    10681 aagttatgca gagtgcatac aaatacgtag atgaattgaa taaggaaaat cctcaattta
    10741 aaattaaaat tgctaatgat acaagttcat acataagtga atcaataaac agtgtaatga
    10801 gcaacttgct tataagtgct gttatcgcat ttattgtaat atttgctttc ctaaaaagtg
    10861 taagagcttc actagttgtt gcagttgcaa taccaatttc tataataggt gctatagcaa
    10921 ttctctactt tactggtgaa agtttaaatc ttattactgc aagttcattg gtaatttcag
    10981 ttggtatggt tgttgataat gctacagttg taattgaaaa tatatttaaa tataggaaaa
    11041 gtggcagatt gagtcttgat gattgtgcta ttgagggaac aagaactgta acaaatgcaa
    11101 tacttgcatc aacattgacc acaatagcaa tattcttacc tatcatgttt acagaaggta
    11161 tagctaaaat aacttttggg gcattaggta aaacactaat agcttcacta gcattctcct
    11221 ttataggtgc aattacactc gttcctagtg tatttgcaaa attaagcaga ggaaaaaata
    11281 gtcaaaagat gcaagaaaaa gatagtccta tatttgataa ggtaagtgaa gcatataaaa
    11341 aattattaag tgtatgtttg agacatcaac gcttagttgt aatatcaagt ttacttattt
    11401 ttgtagcttc cttatttgga atgggattta taggtatgga ctttatgcct gcagcagaca
    11461 agggtgaatt atctatttct atagaacttc caaaaggtct tagcctagaa tccaatgatt
    11521 actatgtatc tatgactgag aaaaaagtct cagatattcc agaaattaaa actttaataa
    11581 caactttgac atcagattca aataatagta aagcaagtat agcaattgaa ttagaatctc
    11641 aaaaaaatag aaaaagaact actgatgaaa tagaaaaaga gataattgat agagtggcta
    11701 ctgtaccaga ttgtaaaata aatgtaagtc aaaacagtag tgttatgggt ggagctggag
    11761 gagcagactt cacattagaa atgaaaggtc ctgatttaga tgtattaaaa atactgggaa
    11821 aagaagctga aaaagaattt agctctataa aaggatttag aaatgttgaa acttctctta
    11881 cagatacaac acaagaagct caatttacta tagataaaca taaggctcaa gaatatggtg
    11941 taaataccgc tgaaattgca ggcatgttac gtacagcaat aaatggtgac tccgtaacca
    12001 ctgctactat tgatgattac aaagtggatg taaacctaaa atttaaagca gatactattg
    12061 atagcttaga agatataaag caactaaaag taaactctgc aactggaaaa gaagttcctc
    12121 ttggtgcttt ttcagatata aaaatggcag atggtctaca atctatatct aggactgatg
    12181 gagatttttc tgtaaatata actgctacac ttgacaattt agatgctggt actgctacaa
    12241 aactagcact agaaaaaacg aataaaatgg taattcctaa agattactct ataggtgttg
    12301 gtggtgaaac agaaatgatg aatgaatcta tggcaagtct tggattttca atggtcatag
    12361 ctatagcatt agtttatatg gtaatggtag ctcagtttga atcatttagt aaaccattta
    12421 taatcatgtt ctgtgttcca tttgcattcg ttggagttgt gctttcgctt ctagttagtg
    12481 gtcaaagttt aagtactgtc ggtatgctag gtataatctt acttatcggt attgttgtta
    12541 ataatggtat agttcttatt gactacatag agcaacttag aaaaacaaat cgtaacaatg
    12601 atttaattgg tttggtttca attggttgtg cagcaagatt aagaccagtc ttaatgacta
    12661 cagcaacaac aattttaggt atgttgccaa cagcactatc tcttggtgaa ggtggagaaa
    12721 ctatgcaacc actagccata gttataatgg gtggtctaac tgtatctaca cttataactt
    12781 tggtgcttat acctactata tacttaatat ttgataaatg ggaaaataag ttttcagaca
    12841 aaatgcctaa aatatttggt aagataacag ataaaatagg aagtataaaa cgtaaaataa
    12901 taaagaaacc aaaattgaaa gctaaagttt aaatagaatg gagggtgatt gtattgaata
    12961 aaaaaatgag caaaatagtt gcaattggct gtggtgttgg tataataact gccaatttaa
    13021 tcccagttta cgcagataat aatacttctt ctataaacaa agttgataat tctattttaa
    13081 gtgactcaaa taaaagaaca tctgaaaagg aaattttgac tgctagagaa gctgttagtg
    13141 ctgcacttaa aaatagtgaa aaattaaaaa tgaaatcaga agaaataaaa atgttaaaag
    13201 aaaagttaga agtacaagat gagtttgata gttttactgg aagtgacaac tcgtttccat
    13261 acgaccaaat tggacttctt aaaaatcaaa gtgaacaggc taaaggcttt atggaggacc
    13321 aaatagcaaa tgatattact aataaattta acgatttagt ttcaagagaa aatgagctag
    13381 acaagataaa aaacaatctt gaaataaaaa ctaaagaaat taaagatatg aaattaaaaa
    13441 aagatttagg gttagtaaca tctttagaaa ctgaatcagc tgaacttgaa cttcaaacac
    13501 tacaaaatac tcaaaaagct aaacttcaag aactaaaaaa caatcaagac tactttaagt
    13561 tgttgactaa tatagattta aataattatc aattggataa agaatataga tttgaatctt
    13621 ttagagtagg tggctcagtt gattcatata tggaaggtaa ggttaatgaa tatttaaaat
    13681 acgaccaatt gattttagaa cgtacagaag aaagctttaa tgataaagat gagaacaaag
    13741 cagatttacc agatagacct aatcttacaa gaccagttgc tcctacaaaa ccagagcaag
    13801 gtaacctaac tgatgatgaa tataaggttc ttacggataa gtatgaaaaa gactacaaga
    13861 gatatgaaaa agaagttgaa gcatacaatc ttgaaagaca gacttatgct gctggcctta
    13921 ctacttatgc aaattatctt caacaaaaat ttaataccga aaatggtcta gtaacattag
    13981 aagacagtaa aaaagctctt aaaaaagggt taattgatag ctatgctcaa ttacttgctt
    14041 tagaagatac aattcagatt actaaaaaac aacttgattt atcagaaaaa caattaaaaa
    14101 atactaaatt aagatatgat ttaggattaa ttactctaac tgattataaa aaacaagtgg
    14161 taagtaatga agatgctaaa aatagctatg acactcttat tgtgaattat aactcactaa
    14221 aaaatggtat agaaaaacct tggatactta atactggaaa ataattctgg taaatagaaa
    14281 gattacttta acatacgttt gattaaaaaa tagttatgca cactcgtggc tttagtcatg
    14341 gaagattcag aaggcaaata atagaaatag attagtaaaa atatatttgc ttataaaact
    14401 gtataatttt atagaggcta tctcaaaata gagataagcc tcttttttat tgtttaaatt
    14461 ataaaaatat atttaattcc atacaaaaag agtgccttat gaactaaaaa gttcattttg
    14521 agacaccctc aaaagcttaa ctctaaattt aattattttt tatcaaatgt ctcatctatt
    14581 ttacatctca ccatgttaac aaattcagaa atattatatc ctctaagttt gcatccacat
    14641 ttaggacaat aatccaattt cctagatacc aaagccctac aactaggttc tggacatctt
    14701 atttttagac tctcatctgc tactttaaat ccacatcgtg ggcatatact acctggacgt
    14761 gatgatactt cctttccaca tttaggacac tttgtcaact ttatcccctc cagtaaactc
    14821 aatttaattg atagttgaaa tttctctcta caataatata tgcaatataa ttaaatacgt
    14881 ttcacttaca actatataag ttaaattttt ataataaaaa tcccctatat tcaatttaca
    14941 aatacttaat ttgatacatt gaacaatagg ggattttatt tttatttagt tacatatttt
    15001 gcagtctatt attatctagg attttttata gatgcttgag ctgctgcaag tctagcaatt
    15061 ggcactctaa atggtgaaca agatacatag ttaagtccta cactataaca gaactctact
    15121 gaagatggtt ctcctccatg ctctccacat attcctagtt ttaattctgg tttaacttct
    15181 ctacctaatt tagctcccat cttaactaac ttaccaacac cattttggtc tagtacttgg
    15241 aatgggtctt tttctaatat ttcttcatct acatattgac ctaagaattt accagcatca
    15301 tctcttgaat atccaaatgc catttgagtt aagtcattag taccaaaact gaagaagtct
    15361 gcttcttgtg ctatttcatc agcagttaaa caagctctag gtatttctat catagtacct
    15421 acagtataag gaacttctat accttcttct tttattatag cgtcaacagt ttctactatc
    15481 atttttctta ttatttttaa ctcatttact tctccaacta atggaaccat tatctctgga
    15541 gaaactttaa cacctgcttt gattgcttct atagcacctt gtattatagc tttagattgc
    15601 attacacata tttcaggata agttattgca agtctacaac ctctgtgtcc aagcattggg
    15661 ttaaactcat ctaaatcaac tattctcttc ttaatatcac ttacttttat tcccatagat
    15721 tttgaaagtt cttctatagt ctcatcatca tgaggcaaaa attcatgtag tggtgggtct
    15781 agtaatctta tattaactgg ttttccatcc ataactttaa ataactctac aaaatcttct
    15841 ctttgcattg gtaatatttt ttcaagagct ttttctcttt gttctactgt attagataat
    15901 atcatttctc taactgctgg caatctagct tcatcaaaga acatatgctc agttctacat
    15961 agacctatac cttctgcacc aaactctata gctgcacgag catctctagg attatctgca
    16021 ttagttctaa ccatcataca tttgtgtttg tctacccagt tcattaattt ttcaaaattt
    16081 ccagttaaag atacttctgt tttcttaaca tctcctaggt atacatttcc tgtagaacca
    16141 tctatagaaa tgtattctcc ttcttttata actacatcat caagtgctct tatttcttta
    16201 ttaaactcat caacttttat ttctccacat ccagcaacac aacatttacc catacctcta
    16261 gcaacaactg ctgcatgtga tgtcattcca cctctagctg taaggattcc ttctgcttta
    16321 accattcctt ctatatcttc tggagatgtc tctagtctaa ctaaaactac tttttctcct
    16381 ttttcatttg cttttactac atcatcagca ttgaaataaa ctttaccact tgctgcacct
    16441 ggtgatgctg gtaaaccttt tgctataacc ttagcttctt ttaatgcctt atcttcaaat
    16501 tttggatgta ataattggtc taattgatta ggctcaattc tcatgatagc ttctttttca
    16561 tcaattattc cagcttcaac taaatcaacc gctacattta ttgctgctgc tgctgttctc
    16621 ttaccatttc tagtttgtag tatatataat ctttcatttt ctatagtaaa ctctatatct
    16681 tgcatatctt tataatgacc ttcaagtata tgagtaattt ttacaaattc atcgaataca
    16741 gctggcatta catctgcaag agttgatata ttttgaggag ttcttatacc tgcaacaacg
    16801 tcttcacctt gtgcattcat taaaaattca ccaaataatt tattttctcc tgtagctgga
    16861 tttctagtaa atgcaacacc tgtaccacta gtttctccca tattaccaaa taccattgat
    16921 tgaatattta ctgctgttcc taagttatga gctatatcat tcaatttacg gtatacgatt
    16981 gcacgaggat tattccatga tctaaataca gcctctattg caagcattaa ttgctctttt
    17041 gggtcttgtg ggaaatcatt ttttatttct tttttataaa ttgctttaaa ttcttttact
    17101 atttctttta aatcatcagt tgtaagttct gtatcaactg tgtatccctt tgcttctttt
    17161 actctgtcta atacattttc aaatttatat tttggaactt ccatagcaac atcagaaaac
    17221 atttgtatga atcttctgta actatcatat gcaaatcttt cattttgtgt agcctctgct
    17281 aagccaacaa cactattgtc attaagacct aggttaagaa ttgtatccat cattcctggc
    17341 atagagaata ctgcaccaga acgaactgat actaataatg gattcttatt gcatcctaat
    17401 gttttattta agtctttttc taaagtagct aaatgctctt ctatttcttt tattatctct
    17461 gctcttatac tttcattgtt aacataataa tcattacatg cttctgttgt tatagtgaat
    17521 ccaggaggga caggtaagcc tatcttagtc atttctgcta aattggcacc tttacctcct
    17581 agtaaagatt tcatgtcttt gcttccttca ccaaaactat aaacatactt agtttccatt
    17641 gttaaatccc ccattaatct ttttatttta atttatatca tttttattaa tagacattat
    17701 taaatttcta tttctttgta tatctttaga ccattttctt tcattatgtc taaaattata
    17761 cctgctgttt cttctattgc cttatttgaa acgtttatta ctgggcatcc aattcttttc
    17821 attatctctt ctgaataatc taactcctga agtattcttt ctaaattagc atagtttgct
    17881 ttactggaca atcctaaagc ttttaatctt tgggttctta tctcattcag cttttcaggt
    17941 gaattagtaa gacctattat ctttttagta tctatctcaa acacttcttt aggaattggt
    18001 atttctggaa ctaatggaac attagctact tttatatttt tattagctaa atacatactc
    18061 aaaggagttt tagatgttct tgatattccc actaaaatta tgtctgcttg aagaacacct
    18121 ctagggtctt ttccatcatc atatttcact gcaaattcta tagcttcaac tctcttaaag
    18181 taagattcat ctaattttct aattatacca gcttctcttt taggttttct tcctgttctc
    18241 ttgcttattt ctcttagtat atctgtcatt aaatctatac aactaagacc ttctctttcg
    18301 caatatcttt caacaatact gcataattct tcatcaacta atgtatatac aattaaagcg
    18361 ttttcagctt ttgcattctc caaagtttca agtagtactc ctttattaat catgtatgga
    18421 aatcttttta tttcataagt ttcattcaca taaaactgag acatacatgc ctttgcaact
    18481 tgttgtgcag tctcccctac tgaatctgat actgcatata taattaggtt tttcaccagc
    18541 atacctcctt aattgtcaac tatttctaag aatagcttag ttatgttggt ttttgatatt
    18601 ctcccaacaa ctctcatatg atttttgtct tctcccttat attcaacaat cggaatcgaa
    18661 tctacttcat gttctattat ttttcttgaa gctaataaaa cacttgctcc tttagttaaa
    18721 gtgactacat ttggagttct tgtcattatc atacctattg gcattttatt tatatcagaa
    18781 cctcctatag ttgcttttag caagtctttt ctggaaacaa ctccacataa ctcttcattc
    18841 tcatctataa taactatact ccctacatca gataaaaaca tagtgactat tacatcatat
    18901 atactttcat ccttcttagc tagtacaggc atgctcatta tatcctctac tgttttttct
    18961 tttacacttt taccaattaa atttatttca ctaactcctg aataaaaata tccaactttt
    19021 ggtcttgcgt ctaatattcc tgtcattgtt aatatagcta aatcagatct aagtgtagca
    19081 cgagtcacat tcaagctgga ggcaatactt tcactcgtta ttggttcatt ttccttaact
    19141 atatctataa tttttagttg tctttcatta agctgaataa taatcacctt ctttcattgt
    19201 gctatacttt ttatcttttc gcatcttata tagtatatta tataactata tataccattc
    19261 tgtaaacaga ttttttattg aatacgatat attttttact aagttctctc gcttaaagat
    19321 atttattata tattaatggg ggatatttct ttaattaata gaactat
//
LOCUS       DAMJZE010000063        19300 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJZE010000063 DAMJZE010000000
VERSION     DAMJZE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.063"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(70..330)
                     /locus_tag="PC541_001940"
     CDS             complement(70..330)
                     /locus_tag="PC541_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839406.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(593..946)
                     /locus_tag="PC541_001941"
     CDS             complement(593..946)
                     /locus_tag="PC541_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839407.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(963..6284)
                     /locus_tag="PC541_001942"
     CDS             complement(963..6284)
                     /locus_tag="PC541_001942"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839408.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(6301..7284)
                     /locus_tag="PC541_001943"
     CDS             complement(6301..7284)
                     /locus_tag="PC541_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2839409.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(7285..7983)
                     /locus_tag="PC541_001944"
     CDS             complement(7285..7983)
                     /locus_tag="PC541_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2839410.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(7980..9032)
                     /locus_tag="PC541_001945"
     CDS             complement(7980..9032)
                     /locus_tag="PC541_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2839411.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9025..9453)
                     /locus_tag="PC541_001946"
     CDS             complement(9025..9453)
                     /locus_tag="PC541_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2839412.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9453..9779)
                     /locus_tag="PC541_001947"
     CDS             complement(9453..9779)
                     /locus_tag="PC541_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2839413.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9795..11324)
                     /locus_tag="PC541_001948"
     CDS             complement(9795..11324)
                     /locus_tag="PC541_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2839414.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(11606..12028)
                     /locus_tag="PC541_001949"
     CDS             complement(11606..12028)
                     /locus_tag="PC541_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839415.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12028..14481)
                     /locus_tag="PC541_001950"
     CDS             complement(12028..14481)
                     /locus_tag="PC541_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839416.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(14481..14648)
                     /locus_tag="PC541_001951"
     CDS             complement(14481..14648)
                     /locus_tag="PC541_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839417.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(14675..15121)
                     /locus_tag="PC541_001952"
     CDS             complement(14675..15121)
                     /locus_tag="PC541_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2839418.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15247..15675)
                     /locus_tag="PC541_001953"
     CDS             complement(15247..15675)
                     /locus_tag="PC541_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2839419.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15689..16753)
                     /locus_tag="PC541_001954"
     CDS             complement(15689..16753)
                     /locus_tag="PC541_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2839420.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16758..17201)
                     /locus_tag="PC541_001955"
     CDS             complement(16758..17201)
                     /locus_tag="PC541_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839421.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17450..17890)
                     /locus_tag="PC541_001956"
     CDS             complement(17450..17890)
                     /locus_tag="PC541_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839422.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17995..18192)
                     /locus_tag="PC541_001957"
     CDS             complement(17995..18192)
                     /locus_tag="PC541_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839423.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18361..18681
                     /locus_tag="PC541_001958"
     CDS             18361..18681
                     /locus_tag="PC541_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2839424.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18763..19269
                     /locus_tag="PC541_001959"
     CDS             18763..19269
                     /locus_tag="PC541_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839425.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT         5955 a         3463 c         1986 g         7896 t
ORIGIN      
        1 atagggtact atctacaata ctactttatc ttttcctaac aatatataat ctggtgctac
       61 tctttcatgt tatacaccgt tttgtaacat accttttata ccagtaacat cttccttaat
      121 ttcctctaca tctgttttca ttatacccat ttgaacaagt atatttttgt ttatttcttg
      181 ttgttgtgta gataattcta taaaattttc tacagttttt ttatacatgt ctctatcttc
      241 ttttttctcc tgcatagtat ttttaaataa tagagcacat agtataccta ttgcccctag
      301 actactcaat tcggttatta atttatccat tatagcctcc tattgtctaa attttttata
      361 cttatagctt taatttttgt tatattctta catcatgcaa tattatattt tcaggattat
      421 caatttaatc ttctgtactt taaattgtag gtgcaattta tagattgaat aaataataaa
      481 tatatataca atttctaata taacaaaaaa taagtacaag gcttacttat taaagttaag
      541 accttgtact ttacataaaa aaaataacta atacatagtt attctaaatt tactattcaa
      601 ctggtatttc taattctttg catctaattc taacttgctc tctaattact gctggtactt
      661 cttctattgt ttttttgcct cttaaaatta aagctacata tacttctacc attccactct
      721 cacctccaga ttttcttaca ctaaaagagc ttatattact tgcttcattg acagaacttt
      781 gatttaacaa catctcatat atttctgcat ttgctataaa attatttgtt atatcatcat
      841 cttgctttac ttgacctata cttatgtctg aaatgtcttt atactgcatt tctacttttc
      901 taccttgttt gatttgttct attttatttt tatcgaattt aaacaatttt cacacctcca
      961 ttttaagctt gtggctcgtt gctttcagca tcatatctaa aaatcataga tggacgtttt
     1021 atagctgtcc atttatctcc tttttctact atgattttag gctgagcact ttttatgttg
     1081 gcttcttcta attcatagat ttcttggtaa cttgagtctg attttttgat ttcaactagt
     1141 ttcatgtctt tgaataagtc agatttattt atttttactt gaactttagt tttatttatt
     1201 aattgatata taggattgtt ttccatcatg tcatccttta atccctcatt taatgttact
     1261 attaaatcag tgttattttc agtaatgttt atgattgaat atactttatt gtttatcatt
     1321 aaaatagaat ccttttttac tttaagccct gtttctgatt taaatataaa catacttgaa
     1381 tataaatcgc ctgtaggact actggcttta atcagtatct tattttctcc taatactatt
     1441 ttattttgtg gtatctcaaa tataatgcta tttatttcca aattagtctt agtagatatt
     1501 aattcatcat ttataaacat ttcaagtttt tctataacat tattagggtc ttgtactaca
     1561 actctcactt gagatattga tttttcaact atttctatag atgctttact tgtatctact
     1621 tttgttatca tgtatattgt atcatctgaa agctctatat ctaaaatatc atttagatgt
     1681 tcaggaattg gtattttagt aaattcatat aaatcagtaa ttgatataga tattttatac
     1741 atagatttat ttttaccagt tatccataga ctattatcat ttttaattac tagtatgtgg
     1801 tcatcattat gtgacttaat ttctttaaca ttagttaata ctttagtaaa agttcttatt
     1861 atcttatctt tttcactttc tcctagtcca aataatccat ttgtattgta cccttgagcg
     1921 tacaactctc cctcattagt taaaacaagt gtcattgagt tatatatact tatatcttta
     1981 acagaaggta atccagtaac cttttgtaac tctcttacat cattattgtg gccaactccc
     2041 aattggtact cagtattctt tccatatgac catagagtat tatctgtctt aataatatag
     2101 gagtagttat ctccacaagc aacttttttg acatctgata tatttacttc agtaagagtt
     2161 aataattcta tatcttcaag ggtttgatta atttctttat atgaattact tcctattcca
     2221 tataacttgc catccttagc aacagccaat gaatgtgttt ttgatatatc aatatcagat
     2281 acctcaaatg gcaatattat tgaagtatac ttaaaattat ctttaaatat gtttttataa
     2341 tttatatact tattatttgt ttttatatac ttatcattaa acattactag cttttcattt
     2401 ttcttattaa aaactatttt atctgatgtg ttatctgttt taaaatatga atgtagggaa
     2461 ccttcaagag cttcattttt atcattatca tatctatggt gaatacctaa atgattatat
     2521 ctattatctc ctgttgaaga ataaaatttt ccatatatat ctttaataaa tatagtattg
     2581 taactagaaa atacagtatc aacatctttc tttatagata ctttataata tggaactgac
     2641 atatccctat aagattcatc agatatacca taagtatctc tacctgatga tatcaaattg
     2701 ttattattat ctatataaag tgtattatta gctgttacaa aaacatcttt aacatcttcc
     2761 acactatttg tattattatt atttgctata cttccatatc cactaagacc tccaacaaat
     2821 agtttattgt catttgttaa aatggctgaa gtatatccat taactactat tttttttgca
     2881 tttataatat tattaattac tcgtataccc tttatatcat tagtttctgt tgaaaagaat
     2941 accctctctt ttttccctgt agtataaata acattgtcat ttgttataaa aaatgtagct
     3001 tcacttcctg ctgttacaac agattttaca ttctcaatat ttatttttgt aaatgtactt
     3061 atacttcctt cagtaaaccc aaaatatcca tttacaaatc cacatacata acatgtatta
     3121 tcatttttaa caattattga tacatcatta catatataca catctctaac tccatttata
     3181 ggtatacgtg taaatatatt cctacttgta gtatcaccca atcctaactg tccttttcca
     3241 ttattacctg ctccatataa aagaccatca ttagttaata atagagatgt gtttccatat
     3301 acagctacat attttacatt ttcaacattt attttagtaa atacatttct atttacatta
     3361 tctcctattc ctaactgacc agaattattt aaaccaacag cataacaggt attatcattt
     3421 tttatcgcaa atacatgttt actattgtca gtaaatctat ttacatttat agattttata
     3481 ttatcaatat ttattttagt aaataccttt ctatttatct catctcccac tcctaattga
     3541 cctacatcat ttaaaccaac actgtaaagc atattattat tagttaataa atatgtagac
     3601 ttcgaatggc tatatatttc ttgtatatta gaagatattt cactactgct attcttttta
     3661 ttgtaattta tcttattatt taaatttaaa ctactattaa ctgatatacc atgcgtaccc
     3721 ttagagttat atccacatat aaataagctc ttatcagtta atattgcaaa tctagtttta
     3781 tctctactta aatcataagg aggtataaat atatttttta tatttttcat taccttttta
     3841 taattagtgg cattatatcc atcttgtgta tctgaaacat ctagttcttt tgcactttca
     3901 gatatattcc caaaaccata taacgtgtta gaagataaat acaatagagc tttatataaa
     3961 aatatggctt tatcagaggt aataggacct gaatttatat tagatgctgc aatattatta
     4021 gatgattgat tgttaagata attccttcca tattgatgta tataagatga acctccagaa
     4081 ataagtaaat tatctggatt tacatttttt aaattacttg ttgattctcc ataatacaaa
     4141 taggcaaaat ttgtagaata tgatgattgt gtacaccttg tgggataata gttatatatg
     4201 taattactaa tatatccagc atgtgtaatt ccttgttgtt gaactctata agtagaatca
     4261 cttggctttg tccataaagt accatggtat ccagttaaaa aagaaccata atttgtacat
     4321 aatatagcca tatattcata tataataata ttattaattt tagtaatatc atttgtacaa
     4381 gaatttgtac tataacttgc tcctgaagga tttacataaa accagttatt tattaatgtt
     4441 aaatttatat tgactccatc attaggatta acacccaata aatagtttgt atttatacca
     4501 gttgcatata cctttttttc gtcagccaca ataaatgttg cattatatga actatatgca
     4561 tctgttacat tgctaacacc aatctcagta tattgacttg ctgatttatt actaagtcca
     4621 ttaacataac ttgaactctt tccagaagta tatatttgct tattattagt tacaattaat
     4681 gaaaaccagt ttgataaatc agtatttata acaacttttt ttacattatc tatagatatt
     4741 ttcttaaata tatctgaatt agaaggaaga acacctgcta ttgagttact atttggccca
     4801 gaataataac aagtgtcatc atttttaatg tagaatattc ctccagaaat attttttgat
     4861 gctactatag actttacatt agagatactt aattttgtca actcatttaa ttgacttggt
     4921 aagtcagaac ccattccttt gtatctatta tttcctagac catatacatc tccattccct
     4981 aaaataacca gagtacaata aggactaaaa acgacatctc ttacagaatg tatatctagt
     5041 ggaagttcaa tatcctttat ttccatttct tcttttaata aagttaattt atcctcagat
     5101 ttaattaaat ttatataatt actataacta tatttttcac tatccatttt cttaaattct
     5161 acaatgtgca tatcatcaaa aaacgaacct aattgtccat aagtattatc tcctgtacaa
     5221 tatgaatcat tatttgaatc aattagtatg ctatgtgata aaccaatgaa aatattctta
     5281 gctacaatat tttttaaatc tataaatgaa ttatctatat tctcagagta atttgctata
     5341 cctaactgat atatatcatt atttccagca accttcaaaa tacctttatt atctataagc
     5401 atagtattac cattaccaca tgcaatagtt tttatattta cttcttctat cttagtaaat
     5461 agtgatgatt gaagtgcatt tccaaaacat ttttcctttc cacaaccata tatatcatta
     5521 ttatacgtca ttaacacagt gtgttcagaa ccacaagcta tagtcttaac atcattaata
     5581 gaaactaagc taaataaatc tctattattc ttatctccta aacctagttg accttgatta
     5641 ttaaaaccag tagaataaca tttattgtca ttctttatta taaaagctga tttattatat
     5701 gagaatacag cctttacatt atcaacatct acttttataa atgtatttac atttgcagat
     5761 gctccattag ttcctagacc taattgacca tacaaattat ttccagcaac atataaagtt
     5821 ccatcattgt ataaaagata tgttgattga agtccacatg caatatcctt tacgcctttt
     5881 atttctggaa ccttctcaaa tgtggaaact gtttttccta ctttttctaa tcccaatccc
     5941 atttgaccat ctgtatttat acctgcgaca agtaattctc catctgaagt aagtataact
     6001 gcatgagatt ctccacatgc aactttttta atattagtag atatatttgg tattttcgta
     6061 aataattttc ttgttgtatt gtcttctccc aatccaagtt gaaagttact attatctcca
     6121 catccataca agatattatt attttttaga ccaaatgtat agttacaaca tccagtaaaa
     6181 aaattaataa agtcataatt atcataccct tcatcaaatt catcaactac catattttta
     6241 tcgccaaaga aattacctct ttgaagtagt ttagttcttt tcattataaa aacctcctaa
     6301 ttatcttatt tcatacgaat atttatttaa agttatatct gtctggccaa ttgaatatgc
     6361 cctattagat tttgtttcaa caaagttatt atccatttca tgtatatatc taactctatt
     6421 tgcattaaat ggtagaacca cattcttaaa tgtaacacta ccaagttctc cagttataca
     6481 ctttctattt aaatctaact taaggccttc tatagctatt atgtttttag cattagttaa
     6541 ggtatcatac caatatccag cttcatctac atctatttct gactctaatt ctaagtatat
     6601 taaaatatct aatacttttt cagataattt attgtaacta ttttttaata cagatagttc
     6661 agtttgtatt tgacctccta ttacatcatc aatagatata tttttaattt tttctactac
     6721 ctcatttata gctcctgata agtttttagc ttctgtagat aagagttctt ctccaatttt
     6781 tacttttatt tcttttatat ttttatcaac cttatcatct aaatcttgaa tatccttttg
     6841 agttacaaaa accaacgtcg ggtctacttc taatgtaaca tttgaagtat tggacaattg
     6901 taaaatcatt tttacaacca aatctttaat actgccatct tcagcacgtg gcttatacgt
     6961 ctctggatac ttacctatag ctattatatt attctcatta tccagaacag cagcttctct
     7021 tatcataaat ccaccaactg ttcctggtaa aatagtctct ataactatcc aattaggatt
     7081 tttttcatca attcttagag aatttatatt tccttcccaa actacattct ttaaagcagt
     7141 ttgttcctct gttggctcat aatattctcc attactatct ccaacttgaa acttggttaa
     7201 tccaacaagc tctcctagtg cagttgcatt tgctattttt tctctaccaa ttttggttaa
     7261 tattatataa aatttttctt ccaattatac cacctccgat ttaggataaa ctactacatt
     7321 ttctacgcct gtatcatttg caatagctat attaatctta caattatttt caatgcattt
     7381 aatgcttaaa ggataaacct ttatttcttc tctacttaac attccagctc cacagtttac
     7441 aacagcgcta cattttataa ttggttcaaa gtatggatga acttttactt cttcactaca
     7501 taccatacct ccaccacaat agaccatact tttatctagt attattggtt ctaaatagtg
     7561 taataagtga gcaggtttta ctctttcaat catatcgcta caatctaaaa gagttttata
     7621 gtcacaatta tttgctataa aactcaatac gaatgtaaat tcatcactat aaacttttat
     7681 atctgtttct gattttgtat atgcctcaca tatacttttt ataacttcaa tagtactagt
     7741 acctctgctt ctcattttag cttttatatt gctacgtctt gtgtcaaaat caagttcttt
     7801 tttttcaatg cagagtatgt cttcccacat atctaatccc catgttgcag tgtcaacaaa
     7861 taattggtta aaggtatcat caatatcttc tttaagaata tttaattctg tatcgtatgc
     7921 accttgtatc tcctctacaa tgtaatttct atcaaatgat ggtagtttat caattagctt
     7981 cattgatttt ccacctcact aaaattaaca gttgttacac taggtatttt atcttcatta
     8041 atagttatat tatctgtact tccatttata agtaggttac ttaaatcgtg tacaccagta
     8101 gtatttataa gtattcccat tacttttgta tagattatct ctcctctaat atctctaaag
     8161 tatgtattta tactttctag gaaagattct tttacattgt ctaatgtata tccatcttct
     8221 aatttcatta ctgcacttat atttatttct attggtaatg gtgtcacaac tgttatagtt
     8281 ggtccaatag gcttctcttc atctatatgt tgctgacacc tttcaattgt ttctgtatca
     8341 acagcttgat tatttttccc aaatatcaag accttaactg ttcccggacc atcccatctt
     8401 ggataaacct taacattata gactccatct acttccaaag cccattcttc atagtgagct
     8461 ttatttccac ttgtagcttg atttctttgg attttataaa atctttcttt tagttcttca
     8521 tctgtctcta tatctgtacc accttcaaaa cttcttgtgt tagtaatctt tgttactcca
     8581 gatatattat caactagttt aaattcacaa tttgtaggta aattatattt cttaccaact
     8641 tccagagctt gaactgggct attgtcacct tcttcactac caatagttac atcttttatt
     8701 actacaaata gtaaatctct atatgatatt attgtgccat tatttattac agttcctttc
     8761 tctccaataa attcaacttc tccatttgac tctgtaccta actttctata tacaccaaat
     8821 tcattgactc ttttatcgag aaactggtta tatgtgtctt gtataaaagc cattttatgt
     8881 atctttgaaa gttctagata tatctttgaa agttctagat tatttccaga taccatgttg
     8941 tttagaaaag aaccttctcc tttataaata tcaagattaa tattttcaag agttctattt
     9001 tttattactt catatgtctg gtcactatac atttatttcc acctccccat agatagtgga
     9061 tacttttaca tttgcactca atacgtctct gttaaagtta gcacttttta cgtttacttc
     9121 taatatatat ggatttatta gaagggcctc ttttatgaat ctactagctt cactttctgt
     9181 aagacctttt gtatattttt gtcctattag ttctgaaagc tctgtcccat attctaaaga
     9241 gtatatctca tgttcatatc tatttgtctt tatacacttg tatatccaaa cttttaaggc
     9301 ttcttttttt tcaattattt taaagtcacc tttttctaaa ataggttcat ctttttcaaa
     9361 atcccatgcc acttcacgaa agattggcaa ttcttctgtt ttaggtaaga tataatcctc
     9421 tgggacacct ataaaaggaa atattgtact cattataagc tcaccaactt acttacaaca
     9481 gcaaattttt cgcctattct aaacattatt accatatccc cagattcaaa attatctata
     9541 aaaggatttt ttacttcatg ctggtgttct tgatttgttt ctgtgtcaaa tgtttctagc
     9601 tgtctatcaa tcatccaact atctatcaaa atatcttctt tttctaatat gatgttattt
     9661 gtctctattt ttaaatctgg taatttactt ttaatttttc caataaaaaa agaaggttga
     9721 ttatgatact ttccttcttc tcttattatt cctataaatt catttattgg attagccatt
     9781 atatcaccat ccttttataa tactctccta gctgtattaa aatctcttct actacttaac
     9841 ttacttattt taactacatc acctttttgt ggagcatgta aaaattctcc attacctata
     9901 tataatccaa catggctgac tggattatga aaaaatacta aatctcctgc ttgaagattt
     9961 ttttgttcta ctttcttgcc ttttttagat tgttgatttg acgttcttgg caaactaaca
    10021 ttaacttttt taaaacaata gaccattaaa ccagaacagt caaaactact tggtccattt
    10081 ccaccccatt tataaggttt acctagatgt ttttttgctt ctgatattac tattttagct
    10141 ttatcagata cattaccaga ataaccacca ataataattt ttccttttct tcttccaaat
    10201 ttactagctt cttcaacatt tccaaataaa atatctatat ggtatgttcc attcttttct
    10261 atagttattg caggtcctac gtcatttact ttatagactc catcacgacc agaaactcct
    10321 gtaattacct gtatttcatc tccatagttt aataaaggat gtttatttaa aaactctttt
    10381 gtataataag tttgttcata tttaccaacc ataggagcag cgcaagtttt tttagatggg
    10441 tcaagttttt tctttctaca atctgtatct ccaccttctt cttttctagg acagtaagct
    10501 gtaaattctg ctgtaaactc ttttcctcct gcataatctt ctccccctaa gttactttct
    10561 tccttaggtt catcctgtcc tgctgacttt tcatccataa gattttgaaa attaagttca
    10621 agctcaattt gatattctcc attttgccaa gtatgtttgt ctgtatctat ataaaatagt
    10681 cctacaagct ttgtataaga atctttaact tttactcctc taccagttat acaacttaca
    10741 tctccataac ctttaagaga acagcttttt tctatcccat taaactcgct atcaatatct
    10801 acatcttgat tttcttgttg ctgaattact ttttgcatta ttacatttac ttccttaaaa
    10861 atttcattat ctattttttc gctaatcttg cttccatact ggtctaccac tattacttta
    10921 ttttttacat tttccatgct ctccgaaaag gtggtattta taatattaaa tccctcttca
    10981 aacataacat ttaatgtaac agttcctttt tcaataacat taaacttatc taaattggcc
    11041 tctatcatat actttttctt tgtcttttta cttgcttctg tatatgcact cattattgtg
    11101 tcataaccat ttactcctat aaacatcttt gtatacttga cattggtctt tgctattgtt
    11161 ccaactgaaa gcctattttc agcaaatact tgctttgcta tgtcttcaac taacttatct
    11221 ttaaaattgt atgacacttc actttgtgta agtaaaaatc ccatatcttt agatacaaaa
    11281 ctaatttcat tactgcttga atctttagac ctattaatta tcattcctct aaagagttct
    11341 ttctcatcta catagaaaca gactgtacta gctattggta tatctatttg ttggaaattt
    11401 acatcaaatg atgattgtat tattgaaaac tctagtgtcc ttgatggaga tttataatca
    11461 cctgaccatg ataccttgtc tactatgtca gttatgtcat atatacttcc gttttttatg
    11521 tgtacccaaa tcttaataat aaaaaatcac ctgccttttc atttaattct ataagcagtt
    11581 atttataact tattatctat aatagttatt gttttggagt tacttttggt atctttattc
    11641 ttttatactc ttttaaatct aatgtaaaat atacatctcg agtcccatct ctttcttcat
    11701 aactaaaccc ttcaattatg acttccatat ttatatttgt ttccgtaatt gtaaatctta
    11761 atataaaacc ttccttcatc catttttcta tcttatttac acaatcatat ggtgatggaa
    11821 aacctgtata gtcacagaaa gtatagtctt tatttggaaa aaaacttgat attgaaatgc
    11881 ttttaagagc tacaccacca aaagttgcta tttcacctat tttgagtata ctagaagagt
    11941 ttacagcagc ttttccatta atactaaagg aagatggaaa tactggaaat ctaaatctat
    12001 cttcagcttg tctaagccac atttccatta taagacaccc cctaactgtg ccaattggat
    12061 acttgcaact aattttgatg ttatcttttc tatatcagct tcttctctaa tcactattgt
    12121 atcagctaac ttagctatgt ttatatttcc accatttttt ctgcttctat attgatttgc
    12181 ttcttgttta gttaaaacca tttcaccttc atggagtctg gtttgatagt cattataagg
    12241 aacatagttc aatccaaatg cattattacc tttcttacct gaataagctt taccagaatc
    12301 accagccttt gttccagcat atggattatc tttgtaattt tttttactct tttccactag
    12361 atttaaaact gttcctgtga tgcttccatt ttttatctta ttaatcatag tttgccacca
    12421 cttgcatata tctttaacta ttttacatac tttatctaat atgttaaaaa aagctcccaa
    12481 tattggctct ataattttcc atccagtttc caacagaggt cctaaggtct cccatacagt
    12541 tttccaaata acacccagtg tctctataaa attgtttatt tgaggtgcat attcacctaa
    12601 aaaagtaaaa attcttgtga taataggact aacagcttca actacaggtc cgattagttg
    12661 gaatgctgta gttactattg taccaaatat acttattaat ccttgtgcaa ttggtgattt
    12721 attgtttata ctatctagta aattatttac taagtttact actggaagta atgaatctat
    12781 taatgctgta aaatcgatat tttgtaatag tgtacttcct gcgttaacaa ttgcttttgg
    12841 tgcattccct atcattgttg ctgcatgatt ttcaaaacgt tcttgcttat tttcaaccat
    12901 cattttacgc ttttcagcct cattagaaat tcgatattga gtagaatcag aaaactcttt
    12961 tccatttttt acacttctag attgcatctg gtcatatttg ttgccaataa ttatcttacc
    13021 attcattaca ttgcctattt taccagccat tttttcagat aagccagcta aattgcctaa
    13081 tccactttct ataaaagttt ttattttctt tagtccttga cctaaaatat catcaaaagg
    13141 tttaacaata ctcttcaagc cattgttaat gcttgtttga gctcctgata ttatacctgg
    13201 tattgtatta ctcatttctt tagctaaccc catctgcttt acacttatgc cttccaatgc
    13261 ttcatcaaaa gttttataat tttctcctac cagagtatta agagattcca tgtttccttt
    13321 tgctgcactt aagaaagctg ctgatacatc ttgttcacta cttgtattca tatttaaagc
    13381 tctaacatct accatagctt gagttatatc tttttctttt ttcccaccag taatattact
    13441 aatcatttta gctttagttc ccattgcaac aacatcttca gggtcaaaag gcgtatcatt
    13501 tgccattctt actaaactgc cataataatc atcaacatct atttgattaa cttttccagt
    13561 atttcttttc attacacttt ctagaacaac tttctgattt tgttcctgac caagtaaatc
    13621 aacacccttt ttcacagcga cacctatagt agcaccattt gtcaaatcag atatcttccc
    13681 ttttatcgaa ccaagtattc cacttgccat atcctttaca gctattgcac cactccatac
    13741 cttacctgca aataatctca atttgggaaa tactttactt ataatgccac ttgccatatc
    13801 ttttatagct attacaccac tccaaaaagt atttttaaac aacattaact taggcaaaat
    13861 tctacccata gcactactca ccatatcttt aagattaata cttccttgat aaggacgttt
    13921 ctctacttgt tttaattttc ccaagatact acttataact ctcttagtca tatcttttgc
    13981 ttttattacg atattccaaa ccttgccacc aatctttttt aaatttccaa gtaatttatt
    14041 tttagttttt tctacttcat cacttttagc tttaaatatt aatataatca taatatttga
    14101 attaaactca tttttctttt gaatcaactt attaaaataa tcaataatca tagctatagt
    14161 tattgctagt atcataatac ttccatttaa acttccaata actcctgaaa cagcatttat
    14221 gctagaactt aattttgtca tagttgaact tacactacta gcagtagttt gaacttttct
    14281 taacgaggaa tttaaactgt ttactctatt acttgcactt ccaatagaat tttttatgct
    14341 actagccatt acattagtgg aaagtgaaac agccacagtt gctctcctaa aattatcagt
    14401 tactgatata atttgattca tttttggtgt gtaattatct gtaaggtaca tatttatacc
    14461 ttcttctctc acatttccca ttacatacca cctcctgtca aaggacacac tccatatcca
    14521 tgtttcattt tttcaacttc ttcctcaatt tctaaggcga aaaaagcttc aataagcttt
    14581 aattcgcctt tatccattgc ataaaaaagg gacggtctta tacctttttt cttccaataa
    14641 tagtacatta ttgtagtcct ggtatccgtc cctattagtt ttttacctct tctatcactg
    14701 catttttacc ataacccata acatcttgaa gtattctata taagttggta atttcacctg
    14761 gtaataataa ttttcttgct aattcttttg gtgttggagc cttaaattta tccattagct
    14821 ccttattttt aaataaaagc tttccatcca aatcacaaac accttctata attgtaagca
    14881 tctgcatctc cggtaagtct atatccgttt tcatattttc atcaatttta agacaagtat
    14941 tttgtatttc ttcatcttta cttattgtta aagctctaca cattacttta aattcctgcc
    15001 caaatatagt tgataatctc ttaagttcaa ctatagtact cggtctttct aagttttctg
    15061 tatctgaatt taaaagtaaa tctactatgt ttatattttt tgataatcca ttttcactca
    15121 ttattatttg ctcctttatc aatcaaattc aatcaactaa cttttttact tattaactat
    15181 ctgaatttat taagtatata tttactaaat atcagtataa aaccaaatat aaaactaaat
    15241 ataaaactaa attatatcaa gaaaatcata ctcagtaaat gtgaaaggtg cttctatctc
    15301 tccttttacg ccaacctccc aatcagctaa agttaaatca tcaaatgcta tattctttac
    15361 tgctattctt tctgctccat aagaatctgg gtcatttaat ttagatatag caacaaacct
    15421 aggttcagaa cctctcttta tcttttctcc aattaacttg tgcattcttg aactaacgtg
    15481 gtatagagtt attgagcctt ttcctttata tcctatatac tttgtatcag tacccatttg
    15541 acctgctatt ataatatctt cttttgtaaa ttccatcttt gcttgaaatt tctttacttc
    15601 tgctactttg tttccatcaa gccaaagttc tccccaagta ccactcatta catttctagc
    15661 ttccatattt gccatattaa taatcctcct atatttctat tgataaatct atatcttcca
    15721 tagcatcaag tacttttatt tttgctttta aaaatacttt agaacctgtg ttagcttctt
    15781 ttatttcttg taatgtcata taagataaat ctactccttt tgattttaaa tacgattttt
    15841 gtgcttcaaa atctatttca acagtagaat cagattctat aagtgctgat ttttctaatt
    15901 cttctaaata actttttata gctactatca ataaacattt gttgtcataa ctgttagtaa
    15961 cctttcctat atagtcatct attatcacct ttcttatgtc actatgtata atatctaaag
    16021 tgtcaactat ttttattttc tggaacattt ctcctttttc tgctgttaat tcagttaaag
    16081 aatttactcc tctagcaatt cttatagctc ctgcttcttt aataagtata agctctcctt
    16141 tattaacctt tgattctgca tcaactttcg tcatcttagg tatatcgact acatcactaa
    16201 gtttagtata agttactgat tgacttaaag gtgtacctgc tataagtcca gccaccctac
    16261 ttgtaaactc atcaacactg tatttctttt ctccaactaa cacatcttct gtagtaaaat
    16321 taattatccc ttcatgattt cctacaactt ttcctaatac agctttaacc ttaaccttat
    16381 ctatatctct aagtttaatt atccaatttt ttatagcagt cttatcagct tctactgctt
    16441 ttggcataca tagataatta aattccttag tctctaaaaa atctaatgca gtttgaatat
    16501 ctgcttctcc ttctattaca taaactaata acttatttgg agtgttaaca tttcccatca
    16561 aagctaaatt tatatattct ttattttcag cagataaatc aactggtata tcctcttttt
    16621 catgtatttc attaagacct agtgccttag catctttaag taccattgca attattcctc
    16681 tagctgaacg ttctttaaca gttgtagcta gctccttaaa tgatatgttg atacttggta
    16741 atcctatagc catactatca ctctcctaaa tttaaattga tttcttccat attttcatat
    16801 actacatctt cttcatatac actatccaga tagtttagtg taaatgtaaa gttcaataca
    16861 taaattgact cttctttttt aatactttgt tctagctttg ttatatttaa ttttctgtct
    16921 gttaccttta tatttctgtt gaataactta tttaattcat ctgaaacttc atacattcta
    16981 atactctttg actgtggttt tggtaagtat tttatagaga taacaatatc tttgttattc
    17041 gtatttaaca ttacaggtgt acaaataact gatagtatac ttacaaaaaa acaagaccct
    17101 tcagagcctt gcacactttc atcatcaata aaaatacttt cagtaaagtt ttttttgaga
    17161 atctcaataa ttgtttctaa tatttccttg tatttaagca aataatcacc acactatcac
    17221 taaatattct tgcttaatct gataatctta ctataaatga tataagtcat ttttaaatat
    17281 atatctcatt tattcctgtc aagtatcttc tatattttta ggtaatatta ttcttttcaa
    17341 tctatcacct ccttcaatat ggatttcttt cacaataaca ttatctcaca tattttattg
    17401 cctattgttg taatggtgta tataaagtgt tgtaaaattg tcctattttt tataatatct
    17461 cactcaattt aaatatcatc tttttcttta aattgtagca agatgttcta tctaagtgta
    17521 gtttcataga aatatatgtc atattgtttt ttgtcttact attataaaaa agattaaaaa
    17581 attccatttc tatatcattt agacacgtta gagcattttc tatcttctct ttttctattt
    17641 ctatatcttc tttttccatt tttaattttc ttaatctttc ctctcttttt aacacctcat
    17701 tttccacaga agagcttatg ttatatgtgt tactagtctt ttctgtatat accattgctc
    17761 cacatccaga gtattcattc tcacatcttt ttatgtccaa gttaatattc tttatcttta
    17821 tatccaaata cttataatta tgtaatcttt tatctgtttc tttaaataat atatcttttt
    17881 tgttttccat agtacttccc cttttttata tacttattat tagcttcaaa tttacaataa
    17941 tctctttagt gcacagtttt ttactaatct cacaaccttc aagaacatat tgttctataa
    18001 ctgtataaaa aaaacttctt ctatattttg ttctaaaata ctagaaatct tcttagccat
    18061 aagaagtcca ggatttttta tgcctctttc tatttgagat atataatcat ttgtaattcc
    18121 agcctttctg gcaagttgaa tttgtgtcat acctttgcat tttctataat atttcaagtt
    18181 atttttaaac attgttttat acctccatta ctattaagaa tattttgttc ttcctcactt
    18241 agattatata gaattatttg ttctttatca atagatttta gaaaaaaata ttcttttata
    18301 ttttattagt tttatttttt agaacaattt gttataatct atacataagg tggtgttata
    18361 atgtttgcca aaagattaag agaacttaga aaggaatttg gattgactca aagagaactt
    18421 ggagaaaaag taggcgtttc ccaaagagtc ttagggtact atgaaacaga aaacagattt
    18481 cctgatgaac acattttaaa taaattggct gatgtattta atgtatcagt tgattacctt
    18541 cttggaagaa ccttagttaa ggaaaatatt gatacagtag ctgcacatag aaaaaatcca
    18601 catgaagagt tacctgaaga agcacaagaa caacttaatg attatataga atttttacta
    18661 aataaatata aaaaaaaata aaccatatga tgagcagtct atttttctgc tcttttaata
    18721 taattcaaag gcaaacatac attctatttt agggggattt ttatgaataa tttggataag
    18781 ctttttgaat tagcttctca agaagagata ataattcatt ataccactta tattgcaggt
    18841 gatttagaag gactatatat aaacaaacat ggtattaaaa ttatatcatt actcagtaat
    18901 ttaaaacaaa actcaaaaaa attgacatcc atcttagctg aagaacttgg acatcatttc
    18961 actagccttg gatactatgt atcctcttat aacgattatt atacaaaaat tattatagat
    19021 aagtgcgaaa ataaagcctt aaaatgggct tgtgagttct taattactga agaagatata
    19081 ataaacataa ttaactctgg catcacttgc gtttatgaaa tggcagacat acttaatgtg
    19141 gatattactt tctttcaaaa aagattagaa tttctatcat taaaaaaaca gtctttacaa
    19201 cttggaaata ataaatattt aatattaact aatttgccgt atttctacat atttgatcca
    19261 atttcataat aaaaatagta gtaattttaa ctggcttaac
//
LOCUS       DAMJZE010000064        19277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJZE010000064 DAMJZE010000000
VERSION     DAMJZE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.064"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            335..913
                     /locus_tag="PC541_001960"
     CDS             335..913
                     /locus_tag="PC541_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2839426.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="PC541_001961"
     CDS             952..1530
                     /locus_tag="PC541_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2839427.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="PC541_001962"
     CDS             1616..2245
                     /locus_tag="PC541_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2839428.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="PC541_001963"
     CDS             2235..4994
                     /locus_tag="PC541_001963"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2839429.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="PC541_001964"
     CDS             5081..6643
                     /locus_tag="PC541_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2839430.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="PC541_001965"
     CDS             6644..7780
                     /locus_tag="PC541_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2839431.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="PC541_001966"
     CDS             7844..8920
                     /locus_tag="PC541_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2839432.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="PC541_001967"
     CDS             8898..10136
                     /locus_tag="PC541_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2839433.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="PC541_001968"
     CDS             10190..11278
                     /locus_tag="PC541_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2839434.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="PC541_001969"
     CDS             11306..12097
                     /locus_tag="PC541_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2839435.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="PC541_001970"
     CDS             complement(12212..12766)
                     /locus_tag="PC541_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2839436.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="PC541_001971"
     CDS             12962..14164
                     /locus_tag="PC541_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2839437.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="PC541_001972"
     CDS             14221..15201
                     /locus_tag="PC541_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2839438.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15444..16397
                     /locus_tag="PC541_001973"
     CDS             15444..16397
                     /locus_tag="PC541_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2839439.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16387..17343
                     /locus_tag="PC541_001974"
     CDS             16387..17343
                     /locus_tag="PC541_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2839440.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17340..18095
                     /locus_tag="PC541_001975"
     CDS             17340..18095
                     /locus_tag="PC541_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2839441.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18187..19152
                     /locus_tag="PC541_001976"
     CDS             18187..19152
                     /locus_tag="PC541_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2839442.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7616 a         1963 c         3452 g         6246 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat
    15241 acaaataata tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta
    15301 gaagtttcaa ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa
    15361 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
    15421 taaaagattt ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt
    15481 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca
    15541 aaaatcagca taaacttatg atatttatca tgagtagaat accaagatta ataagtatat
    15601 tgattgctgg tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata
    15661 aatttgtatc acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat
    15721 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
    15781 ctcttgtagg gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa
    15841 tctttgtacc attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta
    15901 tcgcgtataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
    15961 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
    16021 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag
    16081 gtcttagtta caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat
    16141 gtgtagtcgt tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat
    16201 ccttgtacat gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga
    16261 tatttgtact tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta
    16321 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
    16381 atgtgaatga agcttagtct caatcaaaaa atttactttg taagtatatt actattcata
    16441 tcaatggcac tttttataat ttatggaata gacatgaatc atttagaata tgcattatca
    16501 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
    16561 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
    16621 tcattgtatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
    16681 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
    16741 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
    16801 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac
    16861 cctaatgaat ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact
    16921 aatgtattgt taatagcatt tataataatt attgccataa taccttttat atatgatgaa
    16981 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
    17041 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
    17101 gcacttattg gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt
    17161 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca
    17221 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
    17281 gtaataaatt ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga
    17341 tgatagagat aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct
    17401 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
    17461 gtacagtact atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag
    17521 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
    17581 aacaatctaa caatataact ttaaagctta caataagaga attagttgga tttggaagat
    17641 ttccatatag tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag
    17701 aatatatgaa gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa
    17761 gacaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
    17821 aaccactgaa caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc
    17881 tttgtgatga attagataag actatagtat tagtaatgca tgatataaat tttgcatcat
    17941 gttattcaga taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag
    18001 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
    18061 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg
    18121 ctatatgatt ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat
    18181 attgatatga ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca
    18241 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
    18301 actatgaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta
    18361 gtggtatttg attattcggc tttagataca atggatgcat taggtgtagc tgagacttta
    18421 gtaggacttc caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat
    18481 gcagatttag gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat
    18541 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
    18601 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac
    18661 aaaatagcaa aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa
    18721 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
    18781 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
    18841 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
    18901 attacttttg aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
    18961 atagctactg gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat
    19021 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
    19081 tggtatgtaa atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa
    19141 gcggtaaatt aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata
    19201 aactaggagt ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac
    19261 aataatatta tctataa
//
LOCUS       DAMJZE010000065        19220 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJZE010000065 DAMJZE010000000
VERSION     DAMJZE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.065"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            35..373
                     /locus_tag="PC541_001977"
     CDS             35..373
                     /locus_tag="PC541_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2839443.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(500..1789)
                     /locus_tag="PC541_001978"
     CDS             complement(500..1789)
                     /locus_tag="PC541_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2839444.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1869..2573
                     /locus_tag="PC541_001979"
     CDS             1869..2573
                     /locus_tag="PC541_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2839445.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2665..3711
                     /gene="selD"
                     /locus_tag="PC541_001980"
     CDS             2665..3711
                     /gene="selD"
                     /locus_tag="PC541_001980"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2713..2715,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2839446.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3729..5141
                     /locus_tag="PC541_001981"
     CDS             3729..5141
                     /locus_tag="PC541_001981"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2839447.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5255..7138
                     /gene="selB"
                     /locus_tag="PC541_001982"
     CDS             5255..7138
                     /gene="selB"
                     /locus_tag="PC541_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2839448.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7243..9981
                     /locus_tag="PC541_001983"
     CDS             7243..9981
                     /locus_tag="PC541_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2839449.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC541_001984"
     CDS             complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC541_001984"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2839450.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11217..12578
                     /locus_tag="PC541_001985"
     CDS             11217..12578
                     /locus_tag="PC541_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2839451.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12666..14555
                     /locus_tag="PC541_001986"
     CDS             12666..14555
                     /locus_tag="PC541_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2839452.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14570..15025
                     /locus_tag="PC541_001987"
     CDS             14570..15025
                     /locus_tag="PC541_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2839453.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15084..15401
                     /locus_tag="PC541_001988"
     CDS             15084..15401
                     /locus_tag="PC541_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2839454.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15426..16688
                     /locus_tag="PC541_001989"
     CDS             15426..16688
                     /locus_tag="PC541_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2839455.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16793..17857
                     /locus_tag="PC541_001990"
     CDS             16793..17857
                     /locus_tag="PC541_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2839456.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17911..19089
                     /locus_tag="PC541_001991"
     CDS             17911..19089
                     /locus_tag="PC541_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2839457.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7728 a         1996 c         3364 g         6132 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga aaaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt atctcagtac ttttagattt ataaggataa gtatagtata aattttttat
      481 tataatttcc aactacttgt tactttttca tatttttttt acttctagct ttttttcttt
      541 ttttcttctt tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa
      601 taatcttaac aaaagtcatt aaaacattat tttcagtcaa aaatggaaga ctgctatcaa
      661 gattattttt tgcaattaag tctatactgt tttctagttt accattatta tatacattta
      721 aagtaccaac cttagctcct ttttttatag gcaaatcaat tttatttaac tctgttttgg
      781 tatcataatt gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt
      841 cataaatcaa ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt
      901 tatcaacaat agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt
      961 ctaaatataa atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt
     1021 ttacagcact cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat
     1081 caactatatc atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg
     1141 tcaaaaactt attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa
     1201 atttaagtga cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag
     1261 ctatgagagc catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat
     1321 tattaaaatg agtattagta gctccaattt tttttgcctc agagttcatg aggtcaataa
     1381 atttttgtac attgccacca ccaacatatc tagctaatac aaaagcagca tcattagcag
     1441 aatgaaccaa aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat
     1501 acatactaga gccatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt
     1561 taacctcctt tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct
     1621 tagaatttcc atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat
     1681 gtttagcaac tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg
     1741 aagtaaatat caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc
     1801 aaattttaaa ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg
     1861 attaatatat gaaaaaaaag gtaaaattta tttcgttttt tgttttattt attttaataa
     1921 tatcatcaat aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta
     1981 aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata
     2041 ataatcacag taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta
     2101 tatacataag tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac
     2161 tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca
     2221 atctatcagt aagattagaa gatgaacaac attacataat acctaaaata ggtgaagcaa
     2281 catctgttac aagtaatgat gattccaata aactaaatca gaagaatgag tcaagaggtg
     2341 agcctaaaaa taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag
     2401 cgacgataga agagttggat tctctacctg gggttggaga agctacagcc aataaaattt
     2461 tacaacacag agaagaaaat ggacagttta gttcaattga ggaaataaaa aatgtaaatg
     2521 gtattggaga taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa
     2581 tacaaaactt acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg
     2641 aaatttagta aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca
     2701 acagcaggtg gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa
     2761 ttgcctaaaa atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct
     2821 gcagtttata aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca
     2881 atggtagatg acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta
     2941 tatgctatgg gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat
     3001 gatatggatg tacttgcaga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga
     3061 gcacttttgg ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt
     3121 tctgggattg taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag
     3181 ttaattataa caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg
     3241 gtagaacaac atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca
     3301 gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta
     3361 cttggtcata ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa
     3421 catgtaccaa ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt
     3481 atgtataaaa atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt
     3541 gcagttcaag atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa
     3601 gaagaacttg cagaggagct tgtaaaagat atgaaattaa atggagcaat agaagctaaa
     3661 attataggtt ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata
     3721 gagaaagagt gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg
     3781 aagtattaag tgataataga attgtagaaa taataaatga atatcctaga agtttagttt
     3841 tagaatctgt gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag
     3901 atgcatcaaa ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag
     3961 ttaagttaaa ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc
     4021 atacaaattt aggaaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg
     4081 cttcaaggta ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact
     4141 ctcatttaac tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata
     4201 ataatgcagc ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg
     4261 tatctcgtgg agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc
     4321 ttagtggtgc agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca
     4381 aggaggccat tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa
     4441 taatgggatt cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata
     4501 aactacctgt aatagaagac ttaggaagtg gagtttttat agatttatca aaatatggtt
     4561 tatcttatga accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta
     4621 gtggagataa gatgcttgga ggtccgcaag caggtattat agttgggaaa aaagagtata
     4681 tagaaaaaat gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat
     4741 gtgctcttga agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac
     4801 ctactttaaa aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat
     4861 ttgaaaaaat aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac
     4921 aggtgggagg agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc
     4981 ctgaacatat gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa
     5041 tagccagagt gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat
     5101 ttgatgtaat agttgaggaa ttgagaaaag catttaatta aataaatttg agttaataaa
     5161 tattaaattt agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg
     5221 actttagtca tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc
     5281 aggtcatatt gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga
     5341 tacgttagat gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga
     5401 tttaccaaac aatagaagag ctggaattgt tgatgtccca ggacacgaaa aatttataaa
     5461 aaacatgtta gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga
     5521 aggggttatg ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa
     5581 tggaatagtg gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa
     5641 agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt
     5701 tgattctata tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga
     5761 tgatatagaa gataaaaatg aaaactctcc agcaagactt aatatagaca gagtattttc
     5821 aataaaaggg tttggtactg taataacagg cactttaata gaaggaaaga taagtataga
     5881 agatgatttg gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca
     5941 tggagaaaat aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt
     6001 aaaagtggat gatataaaaa gaggagatgt tttagctgca ccaaactcat tggaggaatc
     6061 aatgatgtta gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg
     6121 ggatagatta agattgtatc atggaactag agagatactt tgtagagcag ttccactaga
     6181 taaagaatta attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat
     6241 agtatctaag aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg
     6301 tggaggtgta ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat
     6361 agaagcatta aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa
     6421 aaggaattta aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga
     6481 agaagatgta aaaagagcgc ttgaaacact tatatcagaa gataaagtat ttattatagg
     6541 aaatatgtat atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt
     6601 aagtgagtac cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc
     6661 taagatagag agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac
     6721 agaaaatgct ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt
     6781 aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt
     6841 ttcttctata ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt
     6901 ggagtcaatg ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga
     6961 tatttatgaa aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac
     7021 attaggagaa tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga
     7081 aaacttcgat agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagca
     7141 gaaaatgtct gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata
     7201 attaattagt ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct
     7261 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
     7321 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
     7381 atatatacga tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa
     7441 gtagattcat tttctacatt tttaggatta atttttatag ctactggagt attggagtgt
     7501 atatatttat ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca
     7561 atatcagcaa taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt
     7621 aaggataaca aacaaattta ttcaagtgta attttattta tagcaactgc tacattaaca
     7681 ataacatctt tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa
     7741 tatgctttag gagttataat atctatattc ataataatta tttctatcat tagtggaatt
     7801 gaattaaatt caagttcaaa taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac
     7861 agaataatag taataataat cttatcaaga gttccaaatc tattgcatat agttattgat
     7921 aatagaaatg tagagaggat attatcacaa ataataataa atgtagcctt atattattta
     7981 tataattaca tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat
     8041 gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag
     8101 acagaaaaga tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct
     8161 acattagatg tagcagtaaa tagcatagta gtatttagca aagatggaga aattacatat
     8221 gcaaataaga gctttagaaa tacttttgga gactataaag aacaagataa attgactgat
     8281 aaaataaaaa acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag
     8341 aatgcagaga aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct
     8401 ttaattattg cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa
     8461 aaggagtacg aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt
     8521 attggagatg gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt
     8581 agggaaattt ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa
     8641 gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt
     8701 tctcagtata ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct
     8761 cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat
     8821 atattacctg atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag
     8881 tcgtttttag atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa
     8941 ctattaatga atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt
     9001 ttaaatgaaa atagaagtat acttttactg gaacatgaat tgataaatag ttatgatgat
     9061 atagtagaag tagaattaag tgcattacct tttgctatat acaatactag atacataatg
     9121 cttattataa aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt
     9181 gaaagattta aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaacta
     9241 aaaactccac taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat
     9301 gaaaagataa aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt
     9361 tcttataggc ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg
     9421 ttctatacac taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg
     9481 aatatagaag agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa
     9541 gaaattaatg tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca
     9601 aactgtatta agtttactga taatggaggc tggatatatg taagtattca ttataaaata
     9661 gataaagtaa tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagag
     9721 ttgatttttg aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt
     9781 ggaatagggc ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta
     9841 gtaagtgaag tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt
     9901 agtgaacatt acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa
     9961 ttttctgata tatattatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg
    10021 tttaaatatt taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat
    10081 ttttagctag gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac
    10141 taaaatctcc aaagtcgttt ggcagtataa aatgcatacc cttttttaaa ttatattcta
    10201 cagaattgtg tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat
    10261 tgtgtgtaaa attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg
    10321 acacaaatgt tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat
    10381 ttatatgagg aacattcgta acatctattg atttttgaac atgaagttca cgcttattac
    10441 ctgaattgtc agttctatca taatcataga ctctataagt agtatctgag ttttgttgag
    10501 tttctaaaat caatgttcct ttgcatatag catgtatagt gccacttgga acatagaaaa
    10561 agtctccctt ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca
    10621 tatttacaaa ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag
    10681 aacaatctat tacataccaa cactctgtct tacctaactc tccattttca ttttttttag
    10741 catattcatc atttggatga acttgaactg ataaattatc atttgcatcc aatatttttg
    10801 taagtaaagg aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca
    10861 attcagatag ttttttacct ttatattttc cattttctat taagcaatct ccatttttat
    10921 gagagcttat agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat
    10981 ctttaagtgc agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt
    11041 ccataatcct gttcctccta tgatattaaa tttatcctac aaataattat atataaatag
    11101 attataaaat caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt
    11161 attaaataag ataaactatg atatataata taaagttcta aattggagtg ataattatgg
    11221 agttgtttaa ggcagaaagc aaaaaatata aaaaagaatt tttaaaatta ttggagcaat
    11281 gggttagtat accttcattt tatgatagaa aaactgtatc taaagacatg ccttttggaa
    11341 aaggagtata tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag
    11401 taaaaaatat agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gagtatgtag
    11461 atatatttgg acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta
    11521 aactaaatat tataggtgat aaattagttg ctagaggtgt atctgataat aaagggccta
    11581 tgatagtgaa ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa
    11641 aggttagatt aatagctggg ggaaatgaag agagtggatt taaatgtata aagcattatt
    11701 atagcaaaga gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa
    11761 atggagaaaa aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat
    11821 atataagtgg tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag
    11881 aaaaattggg taaagataat atatgttttg atattaacaa tatatcgata aattatgata
    11941 atggcaatta tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa
    12001 atcctatatt agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag
    12061 atttatataa gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg
    12121 aggggaaatg tggaatttta tcaatggtgc caacaataat aaatatagta gatggaaaat
    12181 tagaagtagt tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa
    12241 aatttaattt gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt
    12301 taaagcaagc aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat
    12361 atataaagta ttctggagat ttaaaaaatg atgttagagt aactagtgca ggaagttatg
    12421 cttctgaaat gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca
    12481 atgtccatag tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat
    12541 atgcagaggc aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag
    12601 acatattata tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg
    12661 atacaatgat aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat
    12721 atatgacatc aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat
    12781 atatagcaga tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag
    12841 gtataggata caagttaagc ggttctaaaa acaatatagc cagagctgtt aaagaagcaa
    12901 ataaatatat tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat
    12961 gtatgcttat taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa
    13021 gtatagccgc tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact
    13081 tagttataaa atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa
    13141 ggcaattaat tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt
    13201 tagatgacat tagtgaaaac gatatacact gtatagaaag tattttagaa aagcacttaa
    13261 gagaaaaaga tacaataatt tcagataaag attttaatct tttgctttca aaaataatcg
    13321 tttctgtatt taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt
    13381 atagatttca taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg
    13441 gatttaaact tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagcct
    13501 ataaaggtac acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtagtgtta
    13561 taagtagtgc acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg
    13621 agtttatggt tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg
    13681 taaaatcaaa taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt
    13741 tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg
    13801 agcttggata tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta
    13861 gtaagaaaat ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa
    13921 agctaaaaca gaatataagt gatttaagtg ttgtaggtgt ttatccagtt agatatctcg
    13981 atatggctat aagtcaagat gttgatttga tagtatctac cgttgaatta aaaggatatg
    14041 aaaaacctgt cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca
    14101 aagcctttta tgaaaaggaa gaaagaagaa agataataag caacatgttt gatgaaaaag
    14161 cttttttcag tataaaggct aatactaaag aagaggtcat aatttcactt tcaaataaac
    14221 taaaagaaag agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata
    14281 tttcatcaac tgaaatagga aatctagtag caatacccca cacaatagtg aagggagata
    14341 aaaaatctat aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg
    14401 ttcagctagt atttatggta ttttttaata ctaaagaaaa acataatttc tcgatattta
    14461 aataccttta taattttata aaagatgaag gcggagtaag aggaattatt aaaatatgtg
    14521 actttaataa attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa
    14581 catatttaac aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt
    14641 aaaatttata tcaaaaaaag ctaaagactt aaacttggca gaagatgaga gcttagtata
    14701 tgaaggttta atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat
    14761 acctcacact aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga
    14821 agatgttgtt tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc
    14881 aggaaaaagt aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat
    14941 aaataaagaa tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt
    15001 aataaatgaa gctttaggtt tataattaaa cactaaggtt ataaaataaa aaaataatta
    15061 actaaaaata ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgtccaa
    15121 tgggactagc tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag
    15181 gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag
    15241 atgatatatt aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg
    15301 aaagatttga tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag
    15361 aaggtgttat aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa
    15421 atagaatggc aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa
    15481 ataatgtagg aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga
    15541 aagaaataag taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg
    15601 gtggacttat aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat
    15661 ctgtaggtat agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt
    15721 taaatatagc acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac
    15781 tgtttgcagc attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta
    15841 taggtggata tatagctaat aaacctgtag gtgtaccaca attcgttgat ggacaatgga
    15901 ctgaagttgt tccagttgca tcaggatttt taggtgcaat actaatagca tttataatag
    15961 gatattttgt aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta
    16021 aaacaacatt tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta
    16081 taataactcc atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag
    16141 gagcagctgg agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag
    16201 gaggaccaat aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac
    16261 ttccaataac agcaagaaca atagctatag ttgttcctcc aattggatta gggttaacaa
    16321 cattattaga taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag
    16381 gaaaaacttc tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac
    16441 ttgagagacc aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta
    16501 caggtataat tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc
    16561 tagtagataa cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag
    16621 taggaaatat attctataga aataaattaa taaaagatgg taaacttgtt gtagattata
    16681 tcgactagta tttaattttt actatctata aataactttg gctggtaata tggttttaat
    16741 caatattacc agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa
    16801 gatagaaaaa gtaagagagt attttagaga atatgatata gatggatttc ttataaatag
    16861 tcctacaaac aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac
    16921 gaaagaatca caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc
    16981 tagtttgttt aatgtagtat taatggacaa aactagaact cattttgata taattaatga
    17041 tatttgtaga gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga
    17101 tttatataga agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac
    17161 attaagggaa acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt
    17221 tgatgctaca ttttatcata tagtagactt tataaaggtt ggtatgactg aaaagcaagt
    17281 tgaaaatgaa attgttagaa ttataaaaga tttaggtggt caaaaagaat ctttcgatac
    17341 aatagttgca tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaagttat
    17401 agagtatgga gattttgtta cctttgactt tggtgctaag tataataatt attgttcaga
    17461 tattaccaga accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat
    17521 agtaagaaaa gctaatgaag agtgtataag ggttttaaga ccgggaatga ctactggaga
    17581 gatagataaa gttgcaagag acataattgg ttcatatgga tatgctggta attttggtca
    17641 taatctgggt catggtgtag gaattatggt tcatgaatat cctgcattag ctcctgaatc
    17701 aaatgaagta ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag
    17761 tcttggaggc gtaagaatag aagatgatgt acttataaca caagatggat gtatgagatt
    17821 gactacatct actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta
    17881 taacaataag aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga
    17941 gtaagtgaaa agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt
    18001 agagaagatt ttataccaat gtggattgct gatatggatt ttaaaatacc aacagaaata
    18061 gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat
    18121 gaattttatg attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa
    18181 gaatggatta cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt
    18241 tgtaagaaag gtgatagtat aatattaaat acaccagttt atgacccctt tgaatcatca
    18301 gcaaaaaaac aaggtgtaaa tgtaatttgt aatacattag atgttgtaaa tgatagatat
    18361 tatataaatt ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg
    18421 ttttgtacac cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagggta
    18481 gcaactatat gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat
    18541 attcattatg gtacatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt
    18601 attctattga catcaccaaa taaaggattt aatcttggtg gattaaagac ttcttattca
    18661 atagttataa ataaggatat aagggacaaa tttagaaata aactaaagca aaattctata
    18721 acttctccaa atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag
    18781 tggttaagtg gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta
    18841 aataaatata ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat
    18901 ataagtggat taggtattag tgcaagtgag tttacagata aattggctat agatacagga
    18961 gtattattag aagatgggag tcattttgta aaagatggtg agaagtatgt tagaataaat
    19021 ttaggaactc aaaaggaaaa tgtaattgaa gctttaaata gaatggattt atttttaaag
    19081 tctttataaa aatttggttg aattagtatt ttatataaat ttaaactatt aatatggtat
    19141 atacataagt agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt
    19201 aaatttgcat tagtcatgtg
//
LOCUS       DAMJZE010000066        19020 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJZE010000066 DAMJZE010000000
VERSION     DAMJZE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.066"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(45..581)
                     /locus_tag="PC541_001992"
     CDS             complement(45..581)
                     /locus_tag="PC541_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2839458.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(599..1912)
                     /locus_tag="PC541_001993"
     CDS             complement(599..1912)
                     /locus_tag="PC541_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2839459.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2267..2707
                     /locus_tag="PC541_001994"
     CDS             2267..2707
                     /locus_tag="PC541_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2839460.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2891..3079)
                     /locus_tag="PC541_001995"
     CDS             complement(2891..3079)
                     /locus_tag="PC541_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2839461.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4101..4496
                     /locus_tag="PC541_001996"
     CDS             4101..4496
                     /locus_tag="PC541_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839462.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4539..6638
                     /locus_tag="PC541_001997"
     CDS             4539..6638
                     /locus_tag="PC541_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2839463.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6863..12073)
                     /locus_tag="PC541_001998"
     CDS             complement(6863..12073)
                     /locus_tag="PC541_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2839464.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12088..14583)
                     /locus_tag="PC541_001999"
     CDS             complement(12088..14583)
                     /locus_tag="PC541_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2839465.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15244..15531)
                     /locus_tag="PC541_002000"
     CDS             complement(15244..15531)
                     /locus_tag="PC541_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839466.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15551..15826)
                     /locus_tag="PC541_002001"
     CDS             complement(15551..15826)
                     /locus_tag="PC541_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839467.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16461..16640)
                     /locus_tag="PC541_002002"
     CDS             complement(16461..16640)
                     /locus_tag="PC541_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839468.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(16925..18400)
                     /locus_tag="PC541_002003"
     CDS             complement(16925..18400)
                     /locus_tag="PC541_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2839469.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         6323 a         3328 c         2080 g         7289 t
ORIGIN      
        1 taaattttct tttttaataa atctttttct tttaataaat ctatttaatt ttcttcttct
       61 atatcagagc caaagggaat ccatgcatta ccaaactctt tatctttaaa taaataagct
      121 aatccagtta tttgcttcaa tctaagaatt tcatcaactt ccatacccag gtgctttgat
      181 atccattcat cagattttcc aatttcacaa agttcagtta taattttact cattaaatcc
      241 acattatgag aacctcttgc acgattgtgt cgaatagtac ttgccattct ttcatcaaga
      301 gatttatcaa ttactgaaac aggaagaagt ccattttctc tatcatatat atcctcattt
      361 tcaagcatta ctctatatct atgaaaacca tctacaataa tatacttatc cttttcctca
      421 ttataataac agacaattgg cattgtatac ccatcttctt ttatactttt atacaacaac
      481 tgcatttctg gtggggctac tgcatttgga ttatagtcgt tagattctat cttttcgatt
      541 ggcactgcga tgattccata tacaggactt ttatattcca tagcatctta actcctatct
      601 aatgaatttt ttcatatttc ttcttaatat attccgttct aattctctgt tcttttgtag
      661 gaccaaatcc cataaatcta cacatatggt cattttttaa aatacaataa cacattcttt
      721 tccaacttgg tatatctttg gtaaatttaa tatcatccgt atcatcaggt atatcatcca
      781 aaaatatgac tctactcttt ttatcttttg tatgatttga aactccattt aattctattt
      841 tataaccttt tctcaacaat tcacgtataa ctatttctga caaaccacca cctgtattct
      901 gccaaaactc aatagatgtt gaaaattttt taatatagct attccttata ttttcaggta
      961 aggtatcaag caaaaaatat gtgaaacttt tccatgtgtg tccatatggc aatttaacac
     1021 tacgatatcc catagctttt gttttgccat aaatagaagt aaaattaact ccttgtactc
     1081 tagcaaccaa tttcacccat acatctgggt ccaatattct gtataaattt aaactctctt
     1141 ttgctgaatc attaaaaggt gatgccactc tcatttttct tggagctact ccagctttat
     1201 agaataagtc ataaatttta ttatagtcat agtcaaattt atagtgtgct aaccatatat
     1261 cttctatttc ccaatcatat ataggagatg ccgtccagac atttttaaat tccttagtta
     1321 tccagcactt tcctttatat ccatgctgtt tattcacaat agaacaataa cgttgtagag
     1381 attctgctgc tcgtgttcct aataagcaaa caacttttgc accttcatgt atatctctaa
     1441 tccatctacc aaattgtttt gccaaacttt cttgtctcat tttatattta taataagtaa
     1501 aaggattatt tttttgattt attatgtatt ctttgactgg catttccctt acccataact
     1561 cttctttctc atcatcccat ggataccaat acatttcata atttcctaca gcagttcttg
     1621 tagccattgg taaacacacc caatatggtt caatatcttc aatattatct tcaaatactt
     1681 tttcaacata ttcagtagtc aaactatatt gagcctcaaa atcttgatga aatactccta
     1741 ttttggaact tatattattc tttctcttat aatccaaaac taaatttagc agtaaaccac
     1801 tatcttttcc tccgctaaaa gatacataga tataatcaaa ttcattaaaa agataagaaa
     1861 gtctttcttg aaaagcttca aatacatcgg attctaaata tctcttatcc atttgaatta
     1921 tcccctttca attagaagtt tataaaacct tacaatgcaa tattattatg cttttttagc
     1981 agtaatcagc ataaattata cattatttaa atacacaaat ccaactaact acatataatt
     2041 ataatgtaat tatacttttg tattataaac tatattttat cattatttta catttttttc
     2101 caatatatat aaatatcata tttttctatt agctaaaact aattacttta tatttctacg
     2161 tattagtata ataatacata aaaacttgaa taatgcataa aaacttgaca tactgttgtc
     2221 ataattactt tgctataatt aaaagtaaaa atggaggtaa attctaatgt atgaacgtat
     2281 gcttgataaa agcaataaac cctgtttgtc ccaaataata gaatatatag gtaaagatgg
     2341 atatgcacga ttgtgtaagt tggaaactta cttgaaaact caatataatt tatcaagaga
     2401 acttcgattt ccttttggaa acaattatgg ttggggttac aaatatagcc ataaatcttt
     2461 tcatttattt tatgcttttt ttgaaaaagg tgcatttact gttctgattc agataggtga
     2521 caagcaagtt ccagcagtag aaaaaacgtt ggtagctttg acaccaaaag ctaaagaact
     2581 ctgggtcact cgttatcctt gtggcaataa tggaggctgg attaagtatc gtattttaga
     2641 agatgcagac ttacttgata ttattcaata tataagagca aaaaaatctc cacctaaaca
     2701 aaattaactt ttattttttg tgatttaaat atagtgtata taaattccat tctttccata
     2761 ttaaaaaata atataaaaaa tatctattag tataacttat ttagtatgtc ttattctact
     2821 ttttacagat aattaaaaag ttttatcata aataaatcat aactagacat cctagtaaat
     2881 caaaattaat ttattctttt ttattcgaat ttttgataat agtccctata catatgccaa
     2941 ttatcattcc aaaaattaga ccataacaca ttgtaatacc tacatttcct ttagaaatac
     3001 ctatagcaca acctatagcc attccaagta tcatgcctat aaacatacat ataataacta
     3061 aattgttgtt tttattcata gtttttctcc attcaatata ttttaaattg cctagctcgc
     3121 cttcttagct taaaacaaag aaatataaat taactttctt tcattctaat atctgcctgt
     3181 ataaatgtca aacaacacat ttcttgtcat aacctcctct ttttgaagta agtacaatct
     3241 cccagacgca ataatatcaa tttcctaaaa ccatttaagt aacttttata tttacataat
     3301 atttacaatc ataaactata ttgttattca tttatacatc ataaacactc cctcctaatc
     3361 attattatac ttaaaataat gttcatttat aataatatat ctaacgcact ttaattgaaa
     3421 aaactaaaac tttgcctttc attcccatat ataaataagt atactttttg atgctctatt
     3481 acaaatcaac taaattaatt gatataaatt atcattttct gttgaaaata ccattcgcac
     3541 atgctatact taattgcata aatggtaata aatgtcatat gattttacaa atctattaat
     3601 aacaattatt acttttactg tattttaaag tttttggact aaatatcaag aactttctaa
     3661 aatacaaagt acaaaaaatt tatataagtt ataaatttat atgaactaga taagctcaaa
     3721 caatatgtta tgtataaact gtgtagtatt cggttataaa tttatataaa ttatataagc
     3781 tagataaagt attacctcaa ctatataagc tagatatata aattgcttat ataagctaga
     3841 taagaattat ttcaactata taaactacat atataaatta catacataag ctagatatat
     3901 aaactgcata tataagctat ataagaatta tttcaaccat ataaactaca catgtaaaat
     3961 tcatataaat ttactaatat aaatcaaata aaattactaa actctctaaa ctaattttgt
     4021 aaaacatact ctatttttaa catttgttac cttatacata agtacaaaca aaatctaact
     4081 aaatgaaagg ataatgtaaa atgaatattt taaaaatgaa aaaaacaggg atatttttag
     4141 gaggagtact ttttggtact gtaggtgtta agatactttc aagtgattgt gttaaaaaaa
     4201 cttgcactcg tgcaacagca ggagcattga aagttaaaga ttctgttctt gaaacaacta
     4261 cgaaaattca agaaaatgta gaagatattt tagctgaagc aaaagaaatc aacgagcaag
     4321 aagacttatc tacatctaag gtaaacgttg tacaagatac tcttaaatgc gaacaagaag
     4381 ttcttaaaac tgaaactata aaaactgtag aagatactaa ttctaatagc attaaatgtg
     4441 aagaaatcaa agataacgta attgaagaaa aaaatagtac ttgtacaaca gaataaattt
     4501 aatgattatt atgattaata atatcaggag gaagtaaatt gaatttttat ataaaacacg
     4561 aaataagagg tcgtatacgc tttgacttac aacttggcaa attaaacaac aagcagtccg
     4621 atactctctt atattattta acttctataa atggtattac caaagcaaaa gtttacgata
     4681 gaactggtga tgcagtcatt tattacactg gtgaaagaga atacataata agagaaatca
     4741 ctaaattcag ttttaacaat agcaaaatag aaaacctagt tcctgaacat accagcaggg
     4801 aattaaacaa ttattataga gacaaattag caaataaact gctagtcaaa agtatctcca
     4861 aaatattttt tccaaagtcc ttaagatttt ttttaattgg acttcattct ataaaatatt
     4921 ttaaaaaagg tctctcttcc ctgctaaata aaaaaataga agtttctgtt ttagatgcca
     4981 ctgctatagg tatttctatt tttagaaaag atatcaatac ggctggttca gttatgtttc
     5041 ttcttggtat cggtgaacta ttagaagaat ggactagaaa aaaatctata gacgacttgg
     5101 ctcaaagtat gtcactaaac attgaaaaag tatggttaag aaaaggtgac actgaaatct
     5161 taattccaat ttcagagata aaggaaggag atttagtaag tgtaactatg ggaaatatga
     5221 ttccattgga tggagttata gtatcaggag aaacaatggt caatcaggca tcacttacag
     5281 gtgaatccct tgccgttaac aaaaaagaag gctcttacat atatgctgga actgtaatag
     5341 agcaaggcaa tattgttatg tgcgtcaagg aaaaagctgg tactactaga ttccagaaaa
     5401 tagtaactat gattgaggaa tctgaaaaac taaaatcttc tgtagagagt aaatttgaac
     5461 atctagcaga tacattagtt ccatacagct ttttaggaag tatattaacc tatgcaatta
     5521 ctagaaaccc tataaaatca ctctctatat taatggtaga tttctcttgt gcattaaaac
     5581 tctctatccc tatttcagta ctatcagcca tgagagaatg taacaataac aacattactg
     5641 taaaaggtgg aaaattcctt gaaggtgttg catgtgcaga tactattgta tttgataaga
     5701 caggcacatt aacaaaagct caaccaactg tttcagatat aattacattc caaaattata
     5761 ataaggagga tatgttaaga cttgcagctt gcttagaaga acactttcca cactccatag
     5821 caaatgcagt agtttacgaa gctgaaaaac aagggcttag tcacaaagaa atgcatacag
     5881 aggtagaata tattattgct catggcattt caacaaaagt tgatgataaa aaggttgtaa
     5941 taggtagcaa acattttgta tttgaagatg agaagtgtac tatcccagac ggtgaggagg
     6001 ataagtacaa caatctatca gatgagtatt cacacctatt tatggcaata tcaggtaaat
     6061 tatcagcagt tatttgcata aatgaccctt tacgaaaaga agcaaaatat gtaatttcaa
     6121 atttaagaga atgcggaata aaaaagattg tgatgatgac tggagatagc gaaaaaacag
     6181 ctaaatctat agcaagtaaa gttggtgttg atgaatatta ttcagaagtc ttaccagaag
     6241 acaaagccaa ttttgttaaa agtgaaaaac tcaaaggtcg caaagtaatt atgattggtg
     6301 atggaatcaa tgattctcct gctatttcag aatcagatgt cggaattgca atgagtgaag
     6361 gtgctgaaat agcaagagag atttcagata ttaccatatc agcagataat ttgaataatt
     6421 taatcgtttt aaagcagatt agtaataagc ttatgaaaag agttgattta agttctaagt
     6481 ttattattgg ctttaattta ggtctgattt tacttggtgt aggtggattt gttagaccaa
     6541 gtacatctgc ctttttacac aacacatcta ctgtcggaat aagtttaaac agtatgacaa
     6601 atttattgga aaacaccaat acttataact accattaatc tccattacat aaaacatatg
     6661 atataaaaaa tttataattc atctttttaa aagaatatgt cttaaaatta gcttttttct
     6721 aatcaaaatt atataaagca tactttacaa attttaaact caacttattt ctatttttta
     6781 caaaaaagga gatgattcaa attttcattt tgaaacatcc ccttttgtaa atttctttaa
     6841 aatttatata ttaattatat tttcaaccaa taattgtatt ttcaaccaat ctttcttcat
     6901 agcattttaa atcattttgc tttagataat taaattcaga ctctacataa ataccacagc
     6961 ctaccaattc aattgtcaaa ttaggtatat tttcctttag tatactagtc tgctcataaa
     7021 taattctttc caataaatca acttgtaata tagaaaaatg cttcataatc tccccatcac
     7081 tatctatatc tttcttgatt tcataccaac tctttgacaa aattcccttc aaatcaatat
     7141 accctagact cctcaatata tctatactat cgctccatgc atacttacta ggtttatcaa
     7201 agtatgattc tagtttcttc ttctgctcat ttgtaagtga atcataagaa agcttcttat
     7261 taaaatgtgc cttcaaagaa atcttagcca tagaatatgg acttctacca gagtttaatc
     7321 ctgcattttt gaacttttca attcctttat cttctactga agaccatatt gagtttattg
     7381 aatctgaatc attgtattta atatcaagac catgtgtaat atttatatag ttttgtggaa
     7441 atacatattg ataatagaca tcttttttat catcatatag agagaagtat ccactatcat
     7501 ataaatgaat atgcaccttt acttttaaac tatcctctaa aatatttcgt ctaaggttcg
     7561 ggatatttgc atacatggtt acatatttta tggcatcaaa gtttttattt aatggaactt
     7621 caaaactaat ttcatctcct gtcaaaagct ttttcacttc ttcttcatta caatcatata
     7681 gagaatatgt attttcaaat cccacacgtg atttcccatc taaactgcct gttaatgcta
     7741 ccttcaaaat atggttatct gttttttgcc attggtaagc atcttggtca agaaactctc
     7801 taatctgtct attttgctct ttcgcctgat ttataacctc gttttcatct tctgtaatcc
     7861 ctacatggca tctatttaag atatttacta tattgcttcc aaatgggaat gtccatagtt
     7921 gaaaagctgc tgcctcaacc tgacacaaat atctaacctg atttaattca atttgatgga
     7981 taagtttttt ctgatattca ttcatctgat taccactatc tgacatttga taaatcaatt
     8041 ctcccattgc aagattatct tttttcttat caatcttatc agtaatgata atttgagttt
     8101 ccatcatatc ctgtactttt ttaaatatta tagaaatctc ctctgagttt tccattttta
     8161 agcttccaaa cttcgtgctt aattcagtta aaacttcctt catctttaat ttacttatct
     8221 cattttcaaa aatatcttga tttttaactg tactatcgaa tttttctaca aatggtataa
     8281 cagattcttt caaagtttta tctttaaatt ccaccatttg agtattcaat tcatctaatt
     8341 tcttaatatt attttcttta tctttaattg cctctttaac tacctctatt ttactctcaa
     8401 aactagattt tacattaaga caattattaa caccactaat cgcagtattt ataagagaac
     8461 aagcagaact tccaatactc aatgcatttt taattttaga tgtcttttgc tgtctttcaa
     8521 tagcttgttt acttttctct agtgcagcta acttatcttt ttgctgttta ttattgtaat
     8581 ttgcctccaa catacctatt tcttttttct ggatttttgt gtaaatttcc ttatactcgc
     8641 tttcacttaa cccctctttt gctctttgaa tatcgtcagc tttgtctgca tagttatcat
     8701 catctataga cttaactata atctccttaa tttggtcaaa tttagttctt ctttcttcat
     8761 caacacctgc caaattataa gtcattttat ctaaagcatt ttttctatct tcacttagct
     8821 gttctccaag tacaaatctc ataccatcag aatccaattt aaaatctgaa aaatcaacca
     8881 tcctgttagt ccattggtat tcctcttctt tagctgaaag attttccttc aaagattttg
     8941 cactgtcaaa tgcccctaga caagcttgac caacaccact aaatgcagtc agtaaactca
     9001 atgcgctttg tattccagaa agaattaact gtgttttcat ttgttcctca agttttgcct
     9061 gtttcttttt tagttcctct atgtcttctt gttcatcttc tttcatcttt ataatttctt
     9121 gaatcaacag gttcattttt gcattaacag aaagcatttt attctcaagt tcatttttta
     9181 aaacctctat ttgttgattt ccagattcaa tttgagttag gatactttcc ttatatgtat
     9241 tgaaatatcc ttcttttaac tcatctttct ttccttgttc caattctctt aaggtcttat
     9301 ccagatagtc tgtcaaagaa cgaatatctg taacacgatt tcctccaatt tcactttctt
     9361 caatcatctg cacataaaat gctgtcttct gctcaagtgc aaataaaatc atactccatt
     9421 cgctaaaatt ctgatttttt cctacaaaag attctattct atcagactgc atcaatttat
     9481 ttataaggta ttcatagatt tcctttgcct ctttataaaa ttctttcttt atattcatgt
     9541 catccataga aagtactgct ttatttttct tctcaaaact atagtacttc tgaagtattt
     9601 ctctatttaa ttgcatctct attttctcaa ctgcataaaa aatttcataa atatgtgaaa
     9661 attctaaaat atcagcttct aaaattccta ctgcacacaa cagtctaaga tttctataac
     9721 ctttaattcc agaaaaacca aatctctcga aaaattcata gaagaatgta tctattgaca
     9781 aagaatactc ctgctttaac aattcctttt cttttagtgc atctccagag tgaccggctt
     9841 ttccctctgc tccggctgaa cctgaatctt cacgtttgct aattgtccag tccttatgtt
     9901 cttcataaag agtatttaat ttctctccat catctgctgc ttgcttctga tttaaagcat
     9961 gattaaacac atatctatca aaataatagt agtttctatc actattatcc attggaggag
    10021 aggtacctat atatcttatt gtttcttcat atatacctct aattccacca agacctcctg
    10081 caccaccagc tcctccagag cctccttttc ctgctaaacc atgatttcca tcttttttac
    10141 ttatagcact ttttaataaa ggagcttttc caaggagata aacctgtcca gacttgcttc
    10201 caagacctgc atttccacca ttacctcctg tgcctccagt tccaccttgt tttccttttt
    10261 ctgcttttac aacatagtat tttgtacctt taacattagc aaattctttt gactcttttt
    10321 tagaacctat tttagcattt gccccagctt ctccagttat ccctgtatct cctttttttc
    10381 catcttgtcc atttccacca cgtccaccat ctacgtaaag tttaagttct cctctttttt
    10441 ctatttcttt aaaagataat atgatatcac ctgaagtacc tgcattattg ccatttcttc
    10501 cattcattcc gtcgtaaata gaactttctc caaccaattt ttctgcatcc ttaccagata
    10561 cgttgattga ataagtccct tctggtatat gtaatttttc tgtaagtagt acaatattct
    10621 tacctgagaa gacttctttc tccatattac agtcaatttt caactcacaa tctccataca
    10681 tatatataga tgacaaaata ttaagttgat aaccagatat tattttagtc aaatccgata
    10741 aattcacttt aggtatagga atatatatag aaagtacatt ttctttctca tatgatttta
    10801 tatatgtaca actttgaatc ttctctatcc tatactctgc atcttcaagc tttttggctt
    10861 cttcttccct atattcttct tctgctatcc aatatttgat atcttttaat gtatcttcta
    10921 aagtattttt tagcaatgat tttaacaact cacttagacc aacaatatga gtctcactac
    10981 agtaactatt taactctaaa agaagacgtg tttcttctaa tatattcact ttctgtacgc
    11041 catcaccata tgaaagttct tgtacaaact ccataagctc tttcatatta ttagtcctac
    11101 tacataaatc tgtatacatc ttatctaatt gaatcttttt atcttttaga ctatttatag
    11161 aagatagaat ggaaatagaa tcttccatac cttttttaac actttctata taaggaacta
    11221 aaagtttttc taggtcttta taggttacat caacagcttt ttgtatttct attttacaag
    11281 aagctgtaag catcaatccc aaatctgttt tttcaaatgt ttcaggtaga tttttcattt
    11341 catccaacaa cttttctggc tgtttatcta gtggattaca taatttaata gatacaccat
    11401 ctgataatgc tacatttaat aagtacacta attccttaca accgtcgtaa tctttctctt
    11461 gactatctaa gtcttctttt gctttttcca atatatttct tattttttct tctgttacat
    11521 taccgattag tgttttaggt attttattaa taagaaagaa gaaattttct ttatactttg
    11581 cataatcacc aagcatatct ttcaaatttt tgcctgtttc tatcaataat gtatgtctag
    11641 agtccagtag ggattgatag tctaagataa ctaccatccc tttcaccttt gaagatgtgg
    11701 aaatagtttc ccttacaagc atagcaccag caatcatata ctctgctcca cggttgtcaa
    11761 aaaaacctgg gcaatcacat aatgaaaaat ctctatttgc aatatctaca acttctggac
    11821 ataaagtctt agaatataca ccatttccaa tttcgacagc tgaatctttt gcagttatat
    11881 atatttgtcc agtctcttca tccgtttttt cttccatcac acagcctttt aaataattta
    11941 tacaagtact ctttccaacg cctgaagcac ctaggaataa caaaattgac ttttctttta
    12001 agtcatattt tctaagctcg tctcttcctt cttccaaaag gtttacgatt tcactataaa
    12061 gtttatttgc catacatcca tccctttcta tatttcggaa caatagcctt gatatttttc
    12121 tttaataaga ttctcttcta cctttgaaac agctgttgat aacttagtat ttggagcatt
    12181 aaatcctcca ttgtctcctc tatctccata ttcagcgtat tctcttgctg gtcctgtttt
    12241 accttcattc cagcttccac cactaaattc tatatgatat ttaattatac tatctaactt
    12301 ataatcccaa cttgtaaaat atcctgtata gtatacagtc ttatctatac cacttggaga
    12361 aaatgaaagt gtattatatc cgtgatagcc accacttcct ccatatccac ctaaacctgg
    12421 tctaccattt ggacctgctt ctccatcttt cactgttata tcacaatgat taattattct
    12481 atttttttgt tcctctgaat ctgaaatgaa gtaaatatta cctttcttac cagcatttcc
    12541 acctcttcca gcatttccac cagctccacc tttttcacct tttttgccac ttgtaccttt
    12601 ttcaaaatac cctttaaaca tctgtccgtg gaaataactt gtatatcctg catcagctcc
    12661 attacttcca gaagctcctg tttcaccatt ttctccatct attcctttag aaccatttcc
    12721 tccatttgca atcagtgtta gacgataatt tctaatttct tctttaatat ataagtaaat
    12781 attacctgca tctctaccat cttcaccatt tttcccatac atagaattat ctacaagttc
    12841 tccgctcttt ccacttacat taatttttac attactacct acatcaagat tgttagaagc
    12901 aagaataata tttttctctg atgcaaagtt cattccaagg ttatcatcca tataaatagt
    12961 atctttagca aagaacataa aagtagaaac attgtcatct acttttctat ttttgaaata
    13021 ttccaataca ctgctaatta ctaagtttgg aattaatgct cttatttcaa tacctttctt
    13081 atcattaata ttaattgttt ttactataaa tggatttaaa atgtatttac caataaattc
    13141 tctaattatc tcattacctt cttctccata tgcacttaat cttgttatac tgtcttctaa
    13201 ctcttcattt tgtgtaactg taacatttgt caaaattgaa ttaaaatcac ttacactata
    13261 tacataatcc tttttatttg caacttccat taatttttct ctatttggta atgtaaatac
    13321 ctgtttttgc acttcataag aaactaaaac tttttctaaa ttctttctta ttataatgaa
    13381 gttttgttcc attatcttct tacttgcctt ttctataata aattccatat gtacagaagg
    13441 taataaaatt tccccagttc catgttctgt atatttactt aaagcatcaa ttatatctaa
    13501 gtttcctttt atcttatttt gtaatttcat gcaactatct tgtaagaata atccactttg
    13561 tttggctaat acgttgataa aactctctat agtaagactt gttgtacttt ccaaccagtt
    13621 tttaaatact tttacaacct catcatattt tgctatttct tgaatcattg ttatacttcc
    13681 aataatattg ataaaatctt ctgtatacgc cttaacataa tcttctaggc tagatacgat
    13741 attgttttga atttctttta tcaacgtatc taaaactaat cttgcatctg cactaattgg
    13801 atattggaat gctgatgtct tatctttaac ttcatctaat tctagtactt gctttaataa
    13861 gccttccctc tcatctgatg caagtggctt acaaagatga atattttttc cattatcttt
    13921 tagcaattct tcaaataacc aagctttttc ctctatatta ctgtctaaat tttcgcataa
    13981 ttgttcatat tttcttttta taacattata tatttgtttt tctgttcttc ctaagaaact
    14041 ttttgaaatg aaaaaagtta tactaccctt aaaattttct ttatgaagac acatatttaa
    14101 ctgttggaac aggtctagta cttttgtaag tctagaatct aatatatctg cttctggtat
    14161 caatacaatt actgcacata tagatgcaga atttttaaat acttcaccta atgaaactgc
    14221 tgcactaata tcatgggcta ctcccctatt gtcgctaaaa cctgcagtat cacataattt
    14281 tgcatccatt ccttctattg gttttctcat atttacaatg ttcgggtata aagtttgaga
    14341 aacagctcct gaaccaatct ctgccacagg gtctaagcaa tcaatatatg atataccagt
    14401 atcttcatct tctacttcca tcattacaca tcctgcaaga tagttaattg cagtactttt
    14461 gccacatcct gtcccaccaa gaattagaat taaattttga ttttcagcct tatttattgc
    14521 tacttttcct tcatttaata aaagtgttat ttcttctaaa agttgattgt tattttctgg
    14581 catttatttt cccccttggt tatatatttt atttaaataa agtttaatcc cttaagtaga
    14641 ttgcaaagtt tttagactat atttaaattt gttttttatt ttttattttt aataaatttg
    14701 cattttcact aaataattat tcttctttgt ttattaaaat aactgtgttg tataatataa
    14761 tctttatcaa cacagttatt ttatttaatg cgcttatttt attgtatttt atgtaataca
    14821 gttgtttttt atattatatg tccattattt ttctatggtt attacatata gatttttaat
    14881 tatatttgta ccattaattt aaacattcct atactactgc ttaagtttta cacctatacc
    14941 agcttgaata ttttattttt atttgcttaa atagttcaac tatactggtt taaataggtt
    15001 atctttattt gcttaaatag ttcaactata ctggttaaat aggttatctt tatttgctta
    15061 aatagttcaa ctatactgat ttaaataggt tatctttatt tgcttaaata agttcaacta
    15121 tactgattta aataggtttc ctttagttta aataattcac ctatactggt ttaaataggt
    15181 ttcctttagt ttaaataatt cacctatacc tacttgaata gttcactttt atttgcttaa
    15241 attctatacc aatactggct taaacattat actaccatga ttgtattgtc tatcttcaac
    15301 tacaataaaa acaggttcta aatctttagt agatgttatt tttaattcta aaaaaatatt
    15361 atcagttggt aatattttta catcataatt atttaaatta ttattcgtga tactattaaa
    15421 tacatcacct tgacgttcaa aaaaaacatt atcaactatt acaaaataaa catcttcttt
    15481 tctactattt tcaacctcta tagtattttg tttagttata tttagtctca tcttccacct
    15541 cctattaata ttacttaatt atattaatac ttgtaattgt tgcattatgt tcatcacaag
    15601 gaagaggttt aaaaacaact atctctgcta aataattctg tggaggattt gtaactgaag
    15661 tagcattgca aataaattta aaatttgttc ctgctacaac ttgagtagaa acaataagtg
    15721 gttcataacc aaccccttct aacattccta cagcttcatt aaatactttt aaatcttctt
    15781 cttcgacagc tctgaatttt gaccatccac caacaaaaag actcatataa aaacctcctt
    15841 tatatatata tttatttata taaagtagta ccaattttct caatattcaa tattttctgt
    15901 gtaactggtc aatgaaaatg cataactgga taaaaatact tataaaataa cttaacttta
    15961 catataaata gcttacttta catgtaataa gattattcta caatatgaaa tgcatcttgt
    16021 tttgaatata ttctaaatac aataatggat attttcaatt attataatta gaaaatatcc
    16081 attacttaac ttgttttatt taaaatcaca tagagacttt attatttttt ttattgcatg
    16141 aatagctatt tcaggtatca taaggtgata taccccctcc ttgaaaccac aaaaaacatg
    16201 acaaacttta cgaatcttgt cttaaaaaac tacaggtaca ttccttattt gtggattgga
    16261 gtagccataa caagcaaatt ttacaatttt tatgatgaca aaaatcaata aaatcgctta
    16321 gtatataaca cgaaatacta cttgttacta ttaagaatag ttagatagga agtatgcatt
    16381 atacaaataa aggaatcaat aaagaaagtt aaaacatccc tacaaattaa aaatctgtta
    16441 catttttcta tatttaaacg ttatttactt acaattggag aaataacagg ttttccttct
    16501 cggtcaagta aaggcgtaaa tccggctgca ttgccataat agtggaatac atagttcaca
    16561 cctgtttctt tatccaccca aatctccgtt gcatttattt tcccttgtga atatactttt
    16621 tcaaatctat cttttcccat tttataatcc tccatatttt tgattggtct atgccttctt
    16681 cacttaagta gaaaaaatac ataacttatt tttttaattc taatatgagc acgtaaaaat
    16741 gtcaaacagc ttatttaatt tcataacttc atgttactgt tcgtaacctt gtctgtttac
    16801 ttacactgca ttacagcacc attttatcct ttacattatt gtattgcaca cagtaaaaat
    16861 gcttacttta tgggattttt caactaaaaa aaggatttta tctccacaaa acatttacta
    16921 tttgttacaa tatacctaat tttaatctca tatcaacatt taatgaagat ataacctttt
    16981 tcaaatattg taaagactcc ttaaaaatat tagaaaaaga ttctgcaaat ctctcaacat
    17041 ctcctttttt taaataccta agtatctcaa aacatacatc aaaacgtgtt ttcaaaattt
    17101 cattatattc ttcatgaaaa aatctctcta aatagattcc aaatatcata tattgtttta
    17161 aatgcaaaaa tgtttcctgt aatacataaa tttgtgtata atctactatc ttacaaagta
    17221 aaacaaaatc tggataaacc ttacactctt cacaattatt ttgtaagtac cttaaatgag
    17281 tttctatttt atctacagct atcaaatcca ttttatttcc tagtaaaagt actacatcct
    17341 caagagtaat cgtaacaaac tccatagcat ccaaaagcaa actcaacctt ttagctcctt
    17401 cttctgtatt tatataatca tcatcatatt cagaaaaaag cgttacatat gttcccttgc
    17461 cattaattgt tttagcaatt cccaattcat ttagctcatt ataggcactt ctaactgttg
    17521 gaagtgatac attatatcta ttaattatcg aagtaattga tggtagacaa tctcctattt
    17581 taatattgcc ttcaaaaatt tgacaaatta catctgaaac aattaagtca tataaataac
    17641 atttttccaa cgttacagaa ttaaaactac ttgtatctat actttgtgga gcacgatttt
    17701 ctaccatttt aagtatggat tctctacaag ctttatacat atatgttaat agttttttaa
    17761 attcaattag attcccatcc aatccatata agtaagtttt tcttacctgc tctatgtatt
    17821 tattacgaaa aaagtctata caacaattat ccaagttatc caagttatcg aaattatagg
    17881 ccaattgtga aataatgaca tcatgctgaa taatctcaca aaaatggtga atcaggaaat
    17941 tatttgtttt acttattagt ttatttatat attttagcct ttgttcttta aagactaaat
    18001 aattagaact atttttttcc atacaatcga taatctcgtt taattcttca aagtctttag
    18061 atgtaaatct tttaatattg tacatagcaa tagaaggaaa tattaaacta aaaatcttat
    18121 atatttctga tatttgtaaa atcttctcat ataataagtc agattctatt gattgtttac
    18181 tcgtactctg gattaaaact ggtctagaac cagcttttgt ctctataaag cccttttctg
    18241 agagcatttt tattacagtt tttatagtat tttttgatac catatattct tctgacatgc
    18301 tttttataga atcaagccta tctccaatag taattttacc agtataaatt tgttgaatta
    18361 tatgctcaaa aataattaaa tattgtggtt ttctcttcat atttttcctc ttcgataaat
    18421 tagatttatt gttttttgta aggtatattt acttttattt aatagggcaa aatagttgct
    18481 tattttttcc tcataatcta taattaatat aaaccattta tatatatggt ctaagtttta
    18541 taatactgta atgcagtaaa atagcatgtt gtagctatac tacagcaaat taacatacca
    18601 ttgtagtata ttaattggaa taattttaag tacttcaaaa ttccagcaaa acatatatgt
    18661 gtttttgata ttatgtatat tcgttaatag aatagctata cttgttttac ataataaatt
    18721 atatgatact tgctatatgt aatcactata tttaatacac ataactatta tacttgctat
    18781 atgtaattac tatactttat acacatagtt atcatatatg ctatatgtaa ttactatact
    18841 ttatacacat agttatcata tatactatat ataattacta tacttggaca catagttatc
    18901 atacatgcta tatgtaatta ctacacttgg acacataact actatattta ctatatgtaa
    18961 ttatactata cttggacata tagttatcat acatactata tgtaattact atacttgaac
//
LOCUS       DAMJZE010000067        18966 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJZE010000067 DAMJZE010000000
VERSION     DAMJZE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.067"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(372..1439)
                     /gene="rsgA"
                     /locus_tag="PC541_002004"
     CDS             complement(372..1439)
                     /gene="rsgA"
                     /locus_tag="PC541_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2839470.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1436..1837)
                     /locus_tag="PC541_002005"
     CDS             complement(1436..1837)
                     /locus_tag="PC541_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2839471.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2634..2730
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2784..2960
                     /locus_tag="PC541_002006"
     CDS             2784..2960
                     /locus_tag="PC541_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839472.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3370..4008
                     /locus_tag="PC541_002007"
     CDS             3370..4008
                     /locus_tag="PC541_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839473.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(4481..5839)
                     /gene="rlmD"
                     /locus_tag="PC541_002008"
     CDS             complement(4481..5839)
                     /gene="rlmD"
                     /locus_tag="PC541_002008"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2839474.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(6014..7774)
                     /gene="pyk"
                     /locus_tag="PC541_002009"
     CDS             complement(6014..7774)
                     /gene="pyk"
                     /locus_tag="PC541_002009"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2839475.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(7826..8785)
                     /gene="pfkA"
                     /locus_tag="PC541_002010"
     CDS             complement(7826..8785)
                     /gene="pfkA"
                     /locus_tag="PC541_002010"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2839476.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(8994..12563)
                     /locus_tag="PC541_002011"
     CDS             complement(8994..12563)
                     /locus_tag="PC541_002011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2839477.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(12720..13667)
                     /gene="whiA"
                     /locus_tag="PC541_002012"
     CDS             complement(12720..13667)
                     /gene="whiA"
                     /locus_tag="PC541_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2839478.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(13684..14109)
                     /locus_tag="PC541_002013"
     CDS             complement(13684..14109)
                     /locus_tag="PC541_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2839479.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(14178..15284)
                     /locus_tag="PC541_002014"
     CDS             complement(14178..15284)
                     /locus_tag="PC541_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2839480.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(15296..16153)
                     /gene="rapZ"
                     /locus_tag="PC541_002015"
     CDS             complement(15296..16153)
                     /gene="rapZ"
                     /locus_tag="PC541_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2839481.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(16184..16897)
                     /locus_tag="PC541_002016"
     CDS             complement(16184..16897)
                     /locus_tag="PC541_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2839482.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(16901..17815)
                     /gene="murB"
                     /locus_tag="PC541_002017"
     CDS             complement(16901..17815)
                     /gene="murB"
                     /locus_tag="PC541_002017"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2839483.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(18088..18858)
                     /locus_tag="PC541_002018"
     CDS             complement(18088..18858)
                     /locus_tag="PC541_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2839484.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         5981 a         3532 c         2115 g         7338 t
ORIGIN      
        1 ataaatatat tatttgtatt ttataattca tatattttta ctttataaat tagcaatatt
       61 gcagttttgc tcttaagaaa catctctatc atacataagc atatactcta ttatgtatta
      121 gtataaatat gccttttctt atcttacata agacctcttt ataaaggagc ctatcgtaac
      181 aaatatgtat acattatcac atgtatatat catcatataa aaaatcaaac tttattaaaa
      241 aaattaataa attgctttaa ataataccac aaaccaaatt tgccatcaaa atcacattaa
      301 agtaacaaat tgatggcaaa tttaatatat taatagtata tagactcata gaatgaacaa
      361 aaaaactaca cttatcttct aatcttctta tttatttttg agatattttt aaatttcttt
      421 tctttttttg ctaagtattc gcttttattg tctgcaaata ctgcttcagt tttaagccta
      481 cgataggatt cccatctttt aggcgaaagt tcttgatgag caatagcttc ccagatagca
      541 cagcctggtt ctgaggtgtg agtacaatta cgaaaatgac attttccgaa ataagcttct
      601 atatctgcaa aacttttatc taaaccttct gaaacatccc acattcccaa ctctctcata
      661 cctggtgtgt caataacaat accaccacct gaaattaaaa atagctctcg tctagtcgtg
      721 gcatgtcttc ctttatcatc atctatgcga atttcctttg tatttaaaaa ttctccaccc
      781 atcagacaat taattaatga agattttcca actcctgaag aaccaagtaa ggcaacagta
      841 cagcctggtt taatatactt agaaatctgt ttataaccat ctggctctat agatgaggta
      901 acaactacat ctacacccaa ggctatagaa ttaactactt gtaatttacg atgcacattg
      961 tcacataagt ctgatttagt aagtactact actgggatag ctccactttc ccatgttaaa
     1021 gacagatacc tctccaatct atgaatgtta aaatccttat tcagtgacat gcacaaaaaa
     1081 acaatgtcaa tattagctgc aaccacctgc tcctcattta cctttccagc agccttacgc
     1141 acaaacaagc tttttctatg aagcacatga tgaataatag cattaccatt tttattattg
     1201 caacgatcca acataacgaa atcaccaact gtgggaaaat cagattgtat agtagcctca
     1261 aaacgcagct ttcctgaaac ctctgcaaac agtacaccat ttacacatac agcttgataa
     1321 aagttctttt cctgcgagat aatccgagca aggtataaat catgatattc catactttga
     1381 gtaataaaat ttctatttag accatagtct tgtaaattga tttgtttcat accattcatt
     1441 ctgtttcctc cttttccaac ttttcaatga ttctttctag tgggaaaggg ggctgtgcca
     1501 gtggttttaa tgcaattgac attaatttca ctgaattatc atcagctgca atacgattag
     1561 aactaagatg tccacagcgt gtacaacgat gaataattgc ccactcacca ttttttctca
     1621 cccacactcc aataggttcc atattagcat cacaatctgc agcacggtca ccaggaatga
     1681 tatctacatg aagactatgt aaacaatgtg gacaatgatt tctatgttgt gtaccagctc
     1741 cttcaggaac tacaaggcaa ccacaatttt tgcaaataaa gttatccata caggaatttt
     1801 cctgataata agctcgtttt ctatttttat aattcatttt tatttcctcc gtatcgtgta
     1861 ttttctacaa aaaaagtcca caggtattta tatctgcctt tttatgtggt atacatattt
     1921 acctacacaa aaacagaaaa cccctgtgga ctagaaaatt tagtatcttc tatttcaaca
     1981 taattatatt caaaataata ctgtgtaaaa ttaataaaag tgtagcagat taaagcccac
     2041 tacaccacaa tttaacaaag taatatgtaa tattattatt tgttgtctaa tgttagttat
     2101 ctataaaggc agagtaagac acaacaatca aatgtatcaa aaatacactt aaaaattctg
     2161 attaaacaga acccatatgc gtctccaact atttatttgt tggttaccat tatatataac
     2221 tctgacatca ggcaagacac tccttcacta ataattatta ttgaaatttt atcataaaat
     2281 ataagtatgt tcaagtataa tttaatatat tcatatattt atgtttgtct cattttctta
     2341 ttctataaaa tctagtggta ttttgaaatt taatgttcac ctagtattaa taataaaaat
     2401 taactgctat tatttgtttt ctcattttat gcctgttgct caatttatct cacttaaatt
     2461 aataacaact gtcttttcat acaaaaccta caatcaagtt tttccatata ttctacatgc
     2521 atagtctatt ttttttgata taaaataacg ttcatatttg taaatttatt taattttcaa
     2581 ataagtatat tgaataataa gcaattctat tgtattattg taattgtaga ataaaactaa
     2641 atcggcaaaa ctagagaaat ttagtgacgc aaagctatag ggactaacgc ttataaaata
     2701 aactatgagt tatgtcagcc agttgccaaa aagagatatt ctttttgttt ttatgaaagt
     2761 tttttagggg gataataata tatatgttta gagataaaat ggatagatgt acacatatgt
     2821 tgactgctta tattggtagt tcatatgatt attgtgattt tatagataca caactagatg
     2881 attttatact agagtacgga gaaaatgtgg tagaatcttg tttgcatcaa gtgatggtat
     2941 tagtaagtaa gtataattaa atagatttgg ttttctagag aacccttttt agttttatct
     3001 actttaaata gccttatgtt agaaagctaa ttaagataga tttctagtat tcataatttt
     3061 attttttatt taaaatacat ataataaaga tgtaaatttg tatcaaataa taaatatgag
     3121 aacttaaatt ctcttatcta tgtgaaaata aacatctctt aaaatgaaat tctgtaaata
     3181 taaatgattt tttagtttga aggattagat taacaaaatt ataaatttgc aaataagcta
     3241 tattaaaaaa tataataatt ttttatataa cttattcttt tataactttt aattttaaaa
     3301 gaggagaatg tatataagag tattcaatgt actagaaaaa atcaaaagaa atattagaag
     3361 aaatggatat tgagtaatca aagaaaagat tttgatatta caaatgatat gtttcaagaa
     3421 agcattccta tcacaacaaa aattattctt gaagacatgc cttcaaatga tgaattaaat
     3481 catgtttttt caaaaggttg tgaacgaaaa atgaaaaaga gaaaaattgt attaatcaca
     3541 ctactattaa taggtgtttt gttattagga agtatcctat ataatctttt tttaggaaaa
     3601 acagctaata tttcaatgct aaaggaatct tggaattttg atataccaat acctaacaaa
     3661 gaaattgaag tatttgatac tcaagatagt attaatggag atggacaaag ttattttatt
     3721 caaggatttt ctgaaaaaaa tttcaaaaaa gtttttaatt taaaaggtgg tatagttgta
     3781 agcaaagata atattaatga aatagaaaaa tatattgata aatttaaaag agattctgtt
     3841 aacattaata aaagtaataa aaataaaatt gaagaagatt ttaaaaaata taagttagaa
     3901 gttaaaaaag atgataagta tatatataag agaaattatg agaactacgt agttttaatt
     3961 ataaaaaaag atgaacaaaa attatacagt ttaatatgga atcagtaggt acatatgtat
     4021 tattaagaat tataaataat aaaaataaat tttagtcaat atacaaaaac cttttaaatt
     4081 gtctggaaat cacctcttca tttgttataa aaacatacaa caaatgaaga ggtgatttta
     4141 tggttttaaa aaaaataatt ataagtggat atgtgctttc acatgtttat caacatttgt
     4201 aatgcctata tttgctaatg aattataaaa atattaaata atattcaact atagattatg
     4261 taaattactc tatgaaaaaa atgagttgta taaaatctgg ctactgtaga ttgactattt
     4321 cttttaaaat tacaattata ataagtttta tagaattaaa aataaaactt tagagatttt
     4381 tgataacatt gtagactaaa tttgccaccg aaatcgcttt acagtggcaa atcggtggca
     4441 aatacaatat attattagta tataatctat ataaacatta ctattgtttg attatcttag
     4501 ctacagtttc cacatgtgta gaatgaggga acatatctac aggttgtgcc tcaacacatt
     4561 taaatccgtg aacatttaga taatctaaat cccttgctaa agtagaagga ttacaagaaa
     4621 catagacaat tctttccgga ttcattgaaa caatagtctc taataccttt tcgtcacatc
     4681 cctttctagg tgggtctaca acgactacat tggcagtctt accctctcta tacatcttag
     4741 gtacaatttc ctctgccttt ccaacataaa attcagcatt atgtatattg tttaatttag
     4801 catttatctt agcatctttt atggcatcat ctataatttc tattccataa acctttttag
     4861 ccttttgtgc caaaaataaa gatattgtac ctatcccaca gtaaatatca aaaacagtat
     4921 cagtatcatt taaatttgca tattcaagag ccttatcata caatacttta gtttggacag
     4981 ggtttacttg gaaaaatgat aatggtgaaa tctcaaacac taagtctcct atataatcgt
     5041 ttatttttcc atctccatat ataactatat tttctcttcc tagaatagca ttcgtatctt
     5101 tttgatttac atttacaacc aaagtcttaa atccaggtat attttcctta agcactgaag
     5161 ctaattcttt aagatatgga agtttgtttc catttgctac taatacaacc attacttctt
     5221 tagttgtaaa acctattttt gttactaaat gcctaatcaa ccccgtgtga gtcttttcat
     5281 catatatgct taccttatag gcccttatat atgtttttat tattttaaca attttatcat
     5341 ttatctcatg ttgtattacg catttttctg tcgatattac atcatgagtc ttctttttat
     5401 agaagccaat aattggaata ccattattct tttgtattgg aaattgtgct ttatttctat
     5461 atctatatgg attatccatt cctatagtgt catgaacttc tacatcctct aacttaccta
     5521 ttctcgaaag tacttgttta acttcatttg ttttcatttc aagttgttta ttataatcta
     5581 agtcttgtat ctgacaccct ccacaatccc taagattgtc actacacact cttttcaccc
     5641 tgaatggaga cttttctatt atctcaacaa tatctcccac agcataattt ttctttactt
     5701 tattaatcct aactttaact ttatcatgta ttaatccgcc ctctacaaaa actgtaatac
     5761 cattgtactt tcctactcct actccgcctt gaccaatgtc tactatatct acaatataca
     5821 tctcgttttt cgaaagcata aatcaccctc caatcaatct attatattta aatctttaca
     5881 aacttttttc tttaaattta tattaaaata ttgctattaa ttaaactata ttttttacta
     5941 agctacaata ttttatttta ccaaaaaaaa aggagcttat aaagcccctt agtaaataaa
     6001 tttattattt ttcttataaa actctagttg aacctctata taccacgcct cttgatgcat
     6061 ccacagttac aacttctcca tcttttacta gatttgttat atcagctgca gacacaataa
     6121 ctggtatatc taaatttata cctacaatag ctgtatgtga tgtcattcca ccattctcag
     6181 ttactacagc tcctgctttt tctatataat tgttcatttc agcatcagta gcagtagaaa
     6241 caagtatgtc gccttcatta aagtctacac aagcatttcc attctttatt atacgaactt
     6301 taccttctac tgtttgagta cctacaccta taccttgcac gatttcttcg cttattacat
     6361 gaacctttat taaattagta gttccactta ctcctactgg tactccagca gttataacta
     6421 ctaattcccc attatctata taatcagctt gtctagctgc ctcaattgcc ttttctataa
     6481 cttcatctgt atttcctgca acagaagact taactggata tactccccat gtaagagcta
     6541 atttattcat agtcttttca ttatttgtag tagctattat tggagatttt ggtctgaatt
     6601 tagataccat tctagcagta tatcctgaag aagtagatgt tattatagct gatgcattta
     6661 aatctactgc tgttgtacaa gttgcatggc ttattgcatc tgttacagta acattgttag
     6721 taccattttc ctttagtaat ctatcataat ctaaagtctc ttctgttctt tttgctattg
     6781 tagccatcat ttttactgcc tctacaggat attttccagc agcagtttct ccagataaca
     6841 ttatcgcatc tgttccatca tatatagcat tggcaacgtc tgtaacttct gctcttgttg
     6901 gtcttggatt tcttatcata gaatcaagca tttgagtggc agtaacaact ggctttgcta
     6961 attcattaca tttttttatc atcatttttt gaactattgg catctcttca gttggtatct
     7021 caacacctaa gtcacctcta gccaccatta taccatcaga aacttttaat atttcatcta
     7081 ggttctctac accttcttgg ttttctattt tagatattat ttgtatatca gttgcattat
     7141 tgttctctaa tacttctctt attgctagta catcagatgc ctttcttaca aaagaagcag
     7201 ctatataatc tattccttga cttattccaa actctatatc acttatatct ttaggagtta
     7261 ttgctggtaa atttatctta acaccaggta catttactcc tttatgattt ttaactattc
     7321 ctgaattttc aacaacacaa actatatctt ctccatttat ttcttttact cttaagccaa
     7381 ctaaaccatc atctattagt atagaatctc ctgatttaac gtcttcagca agacctttat
     7441 agcttactgt acacatttct ttagtcccca taatatcttt catagttata gtgaactttt
     7501 gcccttcttc taaaaatacc tctgggtctt caaagttacc tgttctaatt tctggacctt
     7561 tagtatctaa taatatagca actggcttat ttaatttttc tctaactttc tttgccatat
     7621 caattctttc tttatgttct tcatgcgaac cgtgagaaaa gttaaatcta cacacattta
     7681 gaccattttg cataagttga gttaaaactt cctcactttg tgatgctgga cctagtgtac
     7741 aaacaatttt tgtcttcttt acattattta acatatttgc tcctcctgag atattattcc
     7801 acagatttat ttttaatact aaaacttata tagataatat ttttgccatc tcataagctt
     7861 ctttatcaaa tactttcttt tgagctaaag cttcacttat ttctaaatca attattttgt
     7921 tttcttttat tcctacaact ctagctgatt ttccatctaa taataattct actgctctta
     7981 ctccaagtct acttgctagt attctatcag atacagttgg gcttcctcct ctttggacat
     8041 gccccaatac agtgactctt aaatctgctc ctgactcttt tttaagttcc ttttctaaat
     8101 cactagcact tccgactcct tctgctaaaa cgattatact atgtctctta cctctttttt
     8161 gagtagtctt taatcttaga gcaacttcat ctactgtaat ttctacttct ggtactatta
     8221 tagtttccgc tcctcctgca agacctgcat ataaggctaa atctccacag tgtcttccca
     8281 taacttctac tatgttaact ctttcatgag atgatgatgt atctcttatc ttacctatag
     8341 catctataat agtattcatt gcagtatcaa atcctatagt ataatctgta tatgctaaat
     8401 cgttatctat agttcctggt atgcctatag ctgggaatcc taagtcacta agtttttggg
     8461 ctcctgcaaa tgaaccatct ccaccaatta caactaagca atcaatttta tactttttaa
     8521 gaatttttac tgcttttaat ctaccttctt ctgttttaaa ttcttcacat ctggaagatt
     8581 ttaatatagt tcctcccctg tgtataatat ctccaacaga agataagttc atttcagtta
     8641 aatcttcttc tagtaatccc ttatatcctc tattgattcc atatactttg catccataat
     8701 atattgctga tcttacaacc gctctaatcg cagcattcat tcctggagca tctcctccac
     8761 ttgttagtaa tcctatagtt ttcatttttt acctccttgg attttatgtc aattttttat
     8821 tataatcatt gttattgtat tctaatggac tttttttgtc cctatatcac tttttaatcc
     8881 catcaagact aaaaaaatta aatgtcctta aatgtccatt ttgtgatatt atatcacaaa
     8941 acggacaata tttttcatat tattataata tatttagatt tttatataaa aaatcaagct
     9001 tttactttta tgttatcttt aggaagtaac tcttctaagt tatttataaa ttcatcacat
     9061 atacaagtca atgccccggt cattttatat gctttacgtt taccatttat attttcatcc
     9121 atagggtata agtatatact gtcattacct ggatattgtt tggctatttc tacaatatta
     9181 ttagttgtat cagtatcaga catactatct attttcaaat acaatttttt accactttct
     9241 ggcttaatat tttctacatt tctagttctt cctgaattgt agtttctatc tgcaaagtca
     9301 gtatcatcat ttatatcttt tatctctcta gctataagtt ttgcattttc gccttctttt
     9361 atacttaatg ttccttttac atatattata ttatcttcat tcaaaatgac attatacttt
     9421 tgtaataatt gaggaaaaac tattatttct atagcaccat acaaatcttc taattcaata
     9481 aatgccataa tttcatttcg ttttgttgtt ttaatagttt tagttacaat cattccaccc
     9541 attataacat ctgtttcatt catttgtagg tatgactctt cttcctcttt taatgaattt
     9601 aattttccat tatccataga tgttttttct tgtagttctt tttcaaattg tgataaagga
     9661 tgtccactta catacattcc caagacttct ttttcaaggt taagtctctc tctttcttcg
     9721 tactcttgcc ttttaggcaa actgtatatt tgttgaaaat cactatcttc ctcactatct
     9781 ccaaaagcat caaataaaga aatttgacct tgaacatttt tctttctatc catagataca
     9841 ctttctaata aactttcata accagccatc agactagctc tattttcact tatattatca
     9901 aaagctccac attttattag actttctatt actctcttat ttgtatcttt ttggtctaat
     9961 cttttgacta aatctactaa atctacaaat aatccattag attctctttc agcgattata
    10021 ttgtttatta tatttacgcc aacatttttt acggctgcta gaccaaatct tatattgtcg
    10081 ccttcaactg aaaactttga aaaacttttg tttatatctg gaggtaatac atctatttct
    10141 agtgctttac attctcttat atactcaact accttatcag tatttcccat tacactggtt
    10201 atcaatgctg ccataaattc aactggataa tatgttttta gataagctgt ctcatatgct
    10261 aaaaccccat atgcagctgc atgtgattta ttaaatgcat atctagcgaa gtctatcata
    10321 tcatcaaata ttttattagc tatttcctct tctacaccat ttctgataca ccctgcaatt
    10381 tctatattac catcttcatc aaatttacca tgaataaaat attgtctttc ttcttccatc
    10441 acgtccatct ttttcttact catggctctt ctaactaggt cacttcgtcc gtaactataa
    10501 ccacctaaat ctctaacgac ctgcataacc tgctcttgat ataccaaaca accatatgta
    10561 acttccatta taggctttaa tttttcatgt agatatgtta catcttttgg gttgctttta
    10621 ttttttatat atgcaggtat agaatccatt ggacctggtc taaataggga tattcccgct
    10681 acaatatctt caaaattgtc tggttttagc tgtttcataa aacttctcat gccagcactc
    10741 tccaattgaa acacccctag tgtatttcct gatgaaagca tttcatatac tttcgggtcg
    10801 tcataatcca tctttgaaaa gtctattttt tctgtgtaat tttttataac tctatttttt
    10861 tctattaaat caagagtatc tcttataact gtaagagttc ttagcccaag aaaatccatc
    10921 tttaaaagcc caagttcttc aagtgttgtc atagtaaact gtgttgtgat tgcatcttga
    10981 tgtttgtaca aaggtacata ttcatctacg ggattttttg atataacaac tcctgctgca
    11041 tgagtagaag catgtcttag cattccctct atctgttttg atatatttat tatttctttt
    11101 gtttccgggt cttggtcata caattcttta agattaggat ttgtatctag ggccttatct
    11161 attgtcattc ctaaagcaaa tggtatttcc ttggctacct tgtcaacttt attgtagcct
    11221 atatccaata cccttccaac atctcttatt gcagcttttg cacccattgt tccaaaagtt
    11281 attatctggg ctacatggtc atcaccatat tttctcttta cataatctat aacttcttct
    11341 cttctctcat aacaaaaatc tatatctata tctggcatgg atatacgttc tggatttaaa
    11401 aaacgctcaa ataatagaga gtactttatt gggtctatat cagttatttt aagtgtatat
    11461 gctactatcg aaccagccgc actacctcta cctggtccta ctataatatt attttcttta
    11521 gaaaaattta taaaatccca aacaattaaa aaatactcta cataacccat tttttcaatt
    11581 acatctaatt cataatttaa tctctctagt atctcattac taggattatc atatctctct
    11641 tttaatccat taaaacacaa ctcccttaaa taagtttctg gtgtatatcc ttctggaaca
    11701 tcatacttag gaagatgtat agtattaaaa tcaaactcca cattacacat atcagctatt
    11761 ttcacagtat tgtcaatagc ctctaatgca tatggaaata attcttccat ttcttctctt
    11821 gattttaaat aaaactcatc actaccaaat ctcattctat ttgggtcatt taatgttttt
    11881 cccatttgaa tacacataag tacatcatgt attttcgaat cttctttatt tacatagtga
    11941 acatcattag tagcaactag tggtatacca gtctcttttg ataattttac taaaccagag
    12001 tttacttctt tttgctcagg taaattgtga tcttgaatct ccaaaaagaa attttcttct
    12061 ccaaatatgt ctctgtagtc aagagcaaat tgttttgctt tttcataatt tctattcatt
    12121 aaagcacgtg ctacatctcc tgcaaggcat gcagaaagtg ctataatccc tttgttatgt
    12181 tttttaagtt cttccatgtc aactctaggc ttatagtaaa aaccttctac gtatgaagtt
    12241 gaaactattt ttattaaatt tttatatcct tccatattct tagctaacag aactaaatgt
    12301 ccttgatatt tgtcatagtt tgggtcttta tctctcaatc cacgtgcagc tgtatagact
    12361 tcacacccta taattggctt tattccttct tttatagctg ttttgtagaa atctataaca
    12421 ccaaacatac aaccatggtc agttatagct attgagctca tgtttagttc cttagccctt
    12481 tttataagtt ttttcaccct cgaaaaccca tcaagtaaac tatactcagt atgtacatga
    12541 agatgagcaa aatctttgtt cattaaatca cctcctgctt tataatttta attttaccac
    12601 aaatatatgt cctaaaacaa aaacagagac ttttatctca aaataggaat taacttcaac
    12661 gtataccatt atatgttata aattaatgct tattctgaga ttgtcccctc aatttattgc
    12721 tatgattttt ttttcaaatc tgttgcaatt tcttctattt ttctaagtct atgattgact
    12781 ccagattttc ctattggtgg ttctaacatc tctccaagtt ctttgagagt catatcctca
    12841 tactcgattc taatcttagc tatctcttgt aaattttctg gcaaactgct aattcctata
    12901 gtctcttgta tgagcttaat attttctact tgtcttactg ctgcatttac tgttttagaa
    12961 aggttagctg tctcacaatt tactatcctg tttacattat ttctcatttc ttttactatt
    13021 ttagtatttt gaagacttaa aagagcgtgg tgtcctccta taatactcaa caaatctgat
    13081 atttgttcac tctctttgat ataaacaacg tgattatttt ttctagccac aattttacta
    13141 ttaaaaccta aagagtttat aagttctttt agagaatctg caaactcctc attattagtt
    13201 acaaattcca tatgatagtt cttttctggg tcgcttatag aacctccacc aagaaaagct
    13261 cctcttatga aagctctctt acatccttca tgcttaatta agttctctga aattttgttt
    13321 atagtaaaaa atccttcttt atcttcaagt atacctaact tttttaataa catttctgaa
    13381 cccatttcgc ttgttaccat taatacataa ctgttatttc tctttagcat ttgattctta
    13441 tttactgaga ttgtagtatt gatattaaaa ttctttttta ataatttaaa tactttacgg
    13501 gctattgaat ttaactctgt tgttatcttt agatttagct tcttatatcc aacaatttgt
    13561 atacttcccc ctgatttaac tagagctgac aattcagcta tattacaaca ttcattccca
    13621 gatataactc ttgctagttc atttttggtt tcagttgaaa aagacatatc tatcacctat
    13681 taactaattt ttctttaacc ttttttttat ttcttgtaag gcaaccttta ttatctcctt
    13741 ttcatcatca tacctagcta agtctactac tctatctagg tggataaatt tagcatcaat
    13801 aaagccttcc tctttttgtg gaatagtatc catattcttt gcttgcatca aataccaaaa
    13861 aactgtttta tgaactgctc tattttcatc ccagttttct ttgaatgtat agtgtatttc
    13921 acctaaatat ttcaatatat ctgcttttac tccagtttct tcacttactt cacgtaatgc
    13981 tgcctcttgg ttattttccc cttcttctac ttttcccttg ggtaacaccc aatctccatt
    14041 gaacttcctt agtaaaagaa tcgtattgcc aaacaggact acaccaccag cactaacctc
    14101 ttctctcatc gatgttcact ccttaaaact aacataatga taaggatttc tacacaattt
    14161 tttaaaatcc ttttttacta acttttgttg taattatgat ttagggctaa ctcaataact
    14221 atgttagata tatactttgc atcatgtctt atataattat tttttatttc aattaaattc
    14281 ttctctacag tttttatacc catctctttt aatttatctt tttgcttttt atctaacaag
    14341 acttgctctg ctccatcttg tttatataag tcaagcattt cttctggcaa tatctcatta
    14401 tttgcgatta cataatctat taaattctca tcagtgtgtt ttactatagc attaacatgc
    14461 tctaatacat tgtacccttc tgtctcacct ggttgagtca ttatatttgc tatatatact
    14521 tttggagcta cagacgattt aatagcgtct acaatcccct ctacaagtaa atttggtatt
    14581 atacttgtat ataaacttcc aggacccata attataatgt cagcatcata aatagatgca
    14641 ataacatcct ttaatggttt tacatctttt ggatttaaaa aaattttatc aatagagctt
    14701 ttttgaattt ttgactcttc aggtatacta gattctccat ttattatatt gccattttct
    14761 aatttggcaa ctaaatttac atcttccaaa gttactggta aaactcttcc tgttatagca
    14821 aaaatttcac ttaatttata tacagctttt tcaaagtttc catacaaccc attcatagca
    14881 gccaaaaaca aatttccaaa actctgacct tttaaaagcc cttctgtaaa cctatattgc
    14941 ataacttcat tcattgttgg ctctatattt gcaagtgcta ataaacagtt tcttatgtct
    15001 cctggtggga gcattcctaa gtcttctctc aatacacctg aacctccacc atcatcagca
    15061 acagttacaa ttgcagttat attttgcgta gtgtgtttaa gacctcttaa aaataccgat
    15121 tgaccagtac ctccacctat cacaaccaca tttggttctc tattttcaac aatagacctt
    15181 tgaaatagtt ttactttttc attttttgct tttttatata taaataacga tacaattaat
    15241 gcaatccaac ctaaaattcc tacaataata agtaatttag ccatgtttgc ctccattatc
    15301 gcaacataga gtctctatgt ttctttacaa ctctatatcc cttattagat aaatcctccg
    15361 ctataagatt tgttatagta actgacctat gtcttccacc tgtgcatcca acaccaatta
    15421 ctagatgttg ttttccttcc tctatatatt gaggtactaa aaaatgaatc atatctaaaa
    15481 gtttcacata aaattcttca ctgattttag aattcataac ataatctcta acttctttat
    15541 catcaccagt tttagctctt aactcttcaa cataataggg atttggaaga aatcttacat
    15601 caaatactaa atctgcatca gcaagtatac catgtttaaa tccaaaagat actacagaaa
    15661 tagttaggtt tggattatct tcaccagaag aatatatttt ctttatttct tccttaaggt
    15721 ctttaggttt catgttagtt gtatcaatta tacatgtaga taactccttt aaaggttcca
    15781 ttatctttct ttccattttt ataccttctg gaatctgcat atcttttgcc aatggatgat
    15841 ttcttcttgt cattttatat ctctttagca atacatcatc attacaatcc aaatatacca
    15901 tttcatattc ataattttct ttttctaaat aatttaagct ttcatgaagc gcttcaaaaa
    15961 actttctccc tcttatatct atccctagag caactttatc aatacttgaa tttggttgat
    16021 aacataattc tgcaaattta gttatcaaag caggaggtag attatctaca caataaaatc
    16081 ccatatcctc taaagctctc attgtctcac tttttccaga tcctgaaaga cctgtaacta
    16141 taacaaattt cattgctaac tcctcccttt tatcttatgt ttattatatt ataatgtttt
    16201 ctactaacga tatatccaga ttagagtcag atatagtctt catagctagt tcaaaatcac
    16261 caactctttc tggacaatgt gcatctgagc ctattataaa tttattttct agccctttta
    16321 ttttatttaa ttgatttaca ttcaaaaaag aatggctact atttatctca agtctagtat
    16381 ttgtcttttt tgctgtcttt gctacttctt ctatatacac atcgccttta tctcctggat
    16441 gagtaattat aaaaatatca ttattactta tcgcatttat tacagctttt gtattatatt
    16501 cttttgcttt ttcagtttta aaaatatagt tattacaatg atttaataag cttcttaatg
    16561 atgttggagt agaaccaaaa tgatatccag ccataatata atcaaaatct ctaataaatt
    16621 tatcatccat atctatattt cctttatcat ctaatatgtt acactccata cctaaaagta
    16681 tatttatatc agtgtactta tcatttaaat aatctacttc ttctctcatt ttgagaatat
    16741 cttttttctt tacaccatat gcaatatgtt tataactatg gtcagatatt cctattgttt
    16801 ttattccttt agaaatggct acctttacat tatcctcaat agtccctttg ccatgactat
    16861 ataatgtatg tgtatgataa tcagccagta ttttcatttg ttaatcctct ccaagtattt
    16921 tgacttcttc ttccaacata accccaaact tggcatatac agcacttttt ataacatata
    16981 ttaaatccaa aatatcttta gcacttgctt ctccttgatt gactacaaat ccacaatgct
    17041 tctcagatac ttgtgctcct cttaaagtaa gacctctcaa tccagtttct tctattaatt
    17101 tacctgcaaa atatcctgtt ggtctcttaa atgtacttcc tgcactagca aaatttaatg
    17161 gctgtttagt tattcttctc gtcgctaaat catccattaa actttttatt tcatcataat
    17221 ttccttcttg taattctaat tctgctgata aagctatata tccgtttttc gatattatac
    17281 tttttctata cccaaattcc atctgttcat ttgataattc aaatatattg ccttccatat
    17341 ccatcagtct tactgacttc actatatcct tcatttcacc accatatgct ccagcattca
    17401 tggctaatgc tcctcccaat gtgccaggta ttcctgatgc aaactcaaac ccttttaatt
    17461 cttgcttttg gagagctttc cctaaaactg ataataaagc tcctgactga gcagtcatcc
    17521 tagtatcatt tatctcataa gaattgaaat tatcagctaa ttctattacc actcctctta
    17581 ttccaccatc tttaattaaa atgtttgaac catttccaat tattaaatac ggaatagact
    17641 ctttttttac caacttaagt acatttttta attgttcttc agttctaggt ctaactaata
    17701 tatctgctgg tcctcctacc ctaaatgaaa tatgtttttt cataggttca tcgactttta
    17761 tatcctcttt acttaatata tttaataaat ttatatatat atcttgatta ttcattgtat
    17821 ttcctcccaa attatactga actttagctg tcattattat ttaaattaca ttaatatata
    17881 gtattaacta tattcaataa tatatgattt tgcttatcat atggatttta tattttaagc
    17941 ttcttaatta caaactatat tcaataaata tataataaag taaaaaaaaa aaaataacaa
    18001 catataaaag aatataaata atttaaaaaa agcatcaata tcactgatat caatgcttta
    18061 aaaggcaatt taattcgtat tatttatcta actatttttg acatagcaaa gataataaat
    18121 ccctccaact actatggaac ctccaacgat atttccaagt gttacaaata ccaaattagt
    18181 tataaattga accatagtta tatttgctcc taatgccatt cccattggta taaaaaacat
    18241 gtttgcaaca ctatgttgga atccacataa tacaaatagc attattactg tccaacatga
    18301 agccaatttt gacattatat cttgagatgc tgtagccatc catacagcta aaacgactag
    18361 aatattacaa agtattgctt ttaaaaacat tgatgctata gttgaattaa ccttagcttc
    18421 tgccacagct atagcttttg tagctgcatc tcctgtcaat gtagaagaat aaaacacaag
    18481 tgcaactagc aaaattgaac ctactaaatt tgcaaaataa accagtcccc agtttcttaa
    18541 catttttcct aatgttattt ttttgtccat aagtgcaata gccattaaag tgtttccagt
    18601 gaataattct gctcctgcca ctactacaag cataagacct actggaaaga ccatagcact
    18661 taaaaagcta gctacccctg ggtctatctt gccaaaagtt tgactaacta tcatactccc
    18721 caatgcccca aaacctataa atgctcctcc aaataaacct aatagaattg cattttgtgt
    18781 cgaaatattt gcttttttaa tgcctgcact tatagttgct tgtgcagttt ccccaggtgt
    18841 caagaatcct ttactcataa atccttcccc cttaaaaatt atactttccc ttgtcttttt
    18901 atggcaataa ttatataata acaatatgat atataataaa ttttattaac ataaaaaata
    18961 tactat
//
LOCUS       DAMJZE010000068        18966 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJZE010000068 DAMJZE010000000
VERSION     DAMJZE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.068"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(159..2129)
                     /gene="ftsH"
                     /locus_tag="PC541_002019"
     CDS             complement(159..2129)
                     /gene="ftsH"
                     /locus_tag="PC541_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2839485.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2163..3551)
                     /gene="tilS"
                     /locus_tag="PC541_002020"
     CDS             complement(2163..3551)
                     /gene="tilS"
                     /locus_tag="PC541_002020"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2839486.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(3734..4162)
                     /locus_tag="PC541_002021"
     CDS             complement(3734..4162)
                     /locus_tag="PC541_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2839487.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4179..4985)
                     /locus_tag="PC541_002022"
     CDS             complement(4179..4985)
                     /locus_tag="PC541_002022"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2839488.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5187..5672)
                     /locus_tag="PC541_002023"
     CDS             complement(5187..5672)
                     /locus_tag="PC541_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2839489.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5723..6382)
                     /gene="spoIIR"
                     /locus_tag="PC541_002024"
     CDS             complement(5723..6382)
                     /gene="spoIIR"
                     /locus_tag="PC541_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2839490.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6527..7207)
                     /locus_tag="PC541_002025"
     CDS             complement(6527..7207)
                     /locus_tag="PC541_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2839491.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7191..8081)
                     /locus_tag="PC541_002026"
     CDS             complement(7191..8081)
                     /locus_tag="PC541_002026"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2839492.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8199..9749)
                     /locus_tag="PC541_002027"
     CDS             complement(8199..9749)
                     /locus_tag="PC541_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2839493.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDNIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9843..10109)
                     /locus_tag="PC541_002028"
     CDS             complement(9843..10109)
                     /locus_tag="PC541_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2839494.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10257..11117)
                     /gene="yabG"
                     /locus_tag="PC541_002029"
     CDS             complement(10257..11117)
                     /gene="yabG"
                     /locus_tag="PC541_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2839495.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11201..12139)
                     /locus_tag="PC541_002030"
     CDS             complement(11201..12139)
                     /locus_tag="PC541_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2839496.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12249..12410)
                     /locus_tag="PC541_002031"
     CDS             complement(12249..12410)
                     /locus_tag="PC541_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839497.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12420..12917)
                     /locus_tag="PC541_002032"
     CDS             complement(12420..12917)
                     /locus_tag="PC541_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2839498.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13032..13742)
                     /locus_tag="PC541_002033"
     CDS             complement(13032..13742)
                     /locus_tag="PC541_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2839499.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13918..14448)
                     /locus_tag="PC541_002034"
     CDS             complement(13918..14448)
                     /locus_tag="PC541_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839500.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14465..14695)
                     /locus_tag="PC541_002035"
     CDS             complement(14465..14695)
                     /locus_tag="PC541_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839501.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14710..16104)
                     /locus_tag="PC541_002036"
     CDS             complement(14710..16104)
                     /locus_tag="PC541_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2839502.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16230..17483)
                     /locus_tag="PC541_002037"
     CDS             complement(16230..17483)
                     /locus_tag="PC541_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2839503.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17534..18688)
                     /locus_tag="PC541_002038"
     CDS             complement(17534..18688)
                     /locus_tag="PC541_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2839504.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
BASE COUNT         5872 a         3617 c         1862 g         7615 t
ORIGIN      
        1 cattttattt aaataatttt aaaataataa aaaaactgaa agtatcaatt aaattaatac
       61 atgcaaatat aatttgctta ttaatttaat attatacttt cagttttagt atttaatcca
      121 catcagataa taatcttaaa atgaccaagg tcttaaaact atttatcttt atcagattta
      181 tcttctaaat ttttatttat atcaataaca ttgtttcttt cttcttcatc tttttctaat
      241 tttatagtta attgttcttc aacttcttta gaagaatttt cctctgtaac tgcattttca
      301 atttcattca gaggtaactc tttattaaat gcttttacaa attgttctgc atctaaagtt
      361 tcatatataa gtaaagcttg tgctacatac tctaatctat ccatattttc ttgtaatata
      421 cttcttgtct tatcatatgc accatcaact atacgctttg tttcttggtc tatctcaaaa
      481 gcaacttctt cagaataatt tctcttactt gaaaaactat tacctaggaa tacttcatca
      541 tcactgtcaa atgacattgg accaagttta gaactcattc catactttgt taccatactt
      601 cttgcagtag ctgtaactct ttctaagtca tttgaagcac ctgtagatac atccttaagt
      661 actagctctt ctgctactct accaccaagt aaaactataa tattttcttt catttcattt
      721 tttgtagcat agaacttatc ttcaacagga agttgcattg taaaccctcc agctcttcct
      781 cttggaacta ttgtaacttg atgaacagga cttacttctt caagtacatg ggcacaaact
      841 gcatgccctc cttcatggta tgctgtaagt cttctttctt tctcacttat tactttcgat
      901 tttttagcca cacctgctat aacctttgtt atagcttctt ctatagtttc catcttaatt
      961 ttcttttctc tttttctagc tgttaatatt gcagcctcat tcattaagtt ctctatatca
     1021 gcaggagtaa atcctggtgt tcttctagcc aagacatcca tttttacatc atcacttaat
     1081 ggtttatttc ttgagtgaac tttaaatata gcctctctac cttttacatc tggtgtacca
     1141 acaacaactt gtctgtcaaa tcttcctggt ctaagtaaag ctgggtcaag tatatctggt
     1201 ctatttgtag ctgccatgat tattatacct tgatttaccc caaatccatc catttcaact
     1261 agaagttgat taagagtttg ctctctttca tcatgacctc caccaagacc tgcacctctt
     1321 tttctaccaa ctgcatcaat ttcatctatg aatatgatag ctggagcact tttcttagcc
     1381 tgttcaaata aatctcttac tcttgaagca ccaaccccaa caaacatctc aacgaaatca
     1441 gaaccactta tactgaaaaa gggaactcct gcttctcctg caactgctct tgatagatat
     1501 gttttaccag ttccaggagg tcctaccata agcattcctt ttggtattct tgcacccaat
     1561 tcaatatatt tcttaggatt ctttaagaaa tctactacct cctgcaaatc ttctttttcc
     1621 tcatctagtc cagctacatc tttaaatgta actctagttt tttcatcatc tttatgaacc
     1681 ttagcttttg atttgccaaa actcataacc tttccgcctc caccttgaga ctgattcata
     1741 aacacgaacc aaataatcac catgaagaat attaacagta atgtaggcaa catttcgaca
     1801 aaccaaggtg tagatggctt agcttcacca ccaaaagtta gtttcccagc cttagcttgg
     1861 tctaacacct catctgcaag tttatttccc atcacttctg ttggaacata tgacttaaac
     1921 tttgtatttg tatcttttat agtaccttct acagaagttt gatttacaaa gtataatctt
     1981 gatatattct catctgttaa ttctctatat acttcagaga attttaaatc ttttattttt
     2041 tctgtaggct tacctgagaa ttgaactata cctactataa ttataaaaac aagtagataa
     2101 aaacctgccc cttttagcaa cttattcaat atagaagccc tcctttcata cattgctttg
     2161 ccctataatt tgttaaaatt aatttttaat acttcttttg tgctttcatc aattttataa
     2221 ttttcactga ttttataatc tccaacacat attattcctt caccatctgt gattatagga
     2281 attttacatc tttcttctct tgggattttt aaatctatga ataaatcttt cactttttta
     2341 cttcccatag caagctttat cttatcacct tgccttctgc ttcttattac tataccccct
     2401 tttaccttat taaaatcaaa ccctttagat gaattgtcta atttcatact cttataccta
     2461 tctatgctca ttacttgggt ctcaataatt tgttttaatt cttttatttt aataaaccca
     2521 ttactaggta cattgtaata aaattcaatt tcctcattaa caatttcctc attcgttaat
     2581 attatactgt ctttttttct ataaacaaat atatttcttg gaagagttaa cattttattt
     2641 acttttgaat tatcttctaa agacattata tcttctatat gtctttggtc aacaaaatta
     2701 gtatctccta gtatatgttt tatggaattt ctaagaactc taactttgat agcatcatgt
     2761 aagcaaacaa aatcgtctaa atttatctct acaaagcctt tttcttttat atttgaaact
     2821 tctctaaatt tagcttctgc ctctttctca atataatcgt tatcactttt taaactattg
     2881 ctcatcctca ctatagattc tattacattt gaattaaaat tatccttcat atatggtaaa
     2941 aggtctaacc ttatcttatt tctagtgtat atattttcta aatttgtttt atctatcctc
     3001 ggatttaaat tataagcttc acaatactcc tctatctcat ttctttccac gtctaatatg
     3061 ggtctaatta tgcaattatc tctaatataa tcaattccct ttaagccttt taaacctgtt
     3121 cctctcatga tacgcatcat aaccgtttcg gcttggtcat tgagattatg acctattgct
     3181 attttattgg ctttaatctt atccttaatt tcataaaaca tttcatatct tagcttcctt
     3241 gctccttctt ctaatgataa tccctcattt tcacaatatt ttggtacatt aatagacttt
     3301 acaaagaata taattcccat atcctcacaa agtttagata catataatgc atctttttga
     3361 gcttctatcc ctcttatttg atggtttaaa tgtgctgcat atatttctat attaaaatct
     3421 ttttttaatc tatttagtac atgcaataaa caaactgaat caggtcctcc agaaagacct
     3481 aaaactattt tatcgccttt ttgtattaaa ttatgtttat ttatagtact tagtacctta
     3541 tcaaatatca tatttatccc tcttacactt agttttattc acttttaagt atactttcta
     3601 taaaaaaacg ctaaaccctt gtatcaattt taagaaatta aagtttaata taaatataat
     3661 aataccaaat aaattaaata aaaggtagtt ttttcactac ctttataaaa tttctggaaa
     3721 atagccatag ttattattta gtacctatgt atatatctat tttattccta cctaaaaata
     3781 aatcttgtac ctttatatta tagtctattt tcaattttat gtgttcccct tcttgtatat
     3841 ttatatctaa ttctcttgta tcaatatcaa ttaataaagt accttgtggg gtcacatact
     3901 ttgtagtact actttctcca catttaaaca gcattgttga gttaatagca ccaaactttt
     3961 tgatactaac ttgattttta agtatcttta tagtattagt tatttctata tcttcttctt
     4021 tttccttgta aataacatat attccatcat ttttataaaa aattttacca aaggcagtca
     4081 tctctatggt gtccatattt cctttttcat catattgtct agtatttata cttattttta
     4141 cactcaacga tttttcctcc atagattctt catataaact agtatttttg tatttctact
     4201 ttttgaatct tatcaatttt tttctttaag aactctcttg ctatatctga aaatttttca
     4261 ggtatatctg atacataata ttgataagtt ccatgaattt ctgtattgtt tataatattt
     4321 tgcacctcta aaatcttttt taaatccttt gcagtctctt tagctggatt aacaagtttt
     4381 atatgctctc caacttcttc acctattgtt ctctttaaaa taggataatg agtacatcct
     4441 aacactaaag aatctatatt tttttctttc aattcttgta aatatatctt tgctgtcagt
     4501 ttagccactt ctgtatttgc ccatccttct tcaacaatag gcacaaataa aggacatgct
     4561 ttattaacaa tttctatact ctcatctatt ttagatattt ccaagttgta tgcttttgaa
     4621 cttatagttc cttcagttcc tattattcct acaattttgt tttttgtaga acttacagct
     4681 gttctagctc ctgcttctat aactccaatt atcggtatat catatttttc ttgagcttct
     4741 tttaagcttc ttgcagtagc agtgttacat gctataacta tagcttttac accttttgtt
     4801 tttaaaaagt ttatagcttg aaaagtatat ttaattatag tttctttgga tcttgaacca
     4861 tatggtattc tggcagtatc tccaaagtat attatatcct catttggtaa tatcttcatt
     4921 atctctttta gaactgttaa tccacctaat cccgaatcaa aaactcctat tggtttattg
     4981 ttcatcttta tacaacacct actttacatt tttgtgttta acaacaactt atttatcgct
     5041 aaaatttata tttatattca taattaatat attattacct aaatgattat tatagtttat
     5101 tttacacaaa aaataaagga gctatcttaa attagaaaat aatttctaca aaaagacaac
     5161 tcctttcact acttaaaaat ttaaatttat tttgcaaaca catgatttcc tattctcata
     5221 taaggattca atctatttat ccatttgcaa gtggctattt ctggattcca aaaatatact
     5281 gctttattag ttgggtcagt tccataaaga gcttcctgag ctgctttacg tgctgagtct
     5341 gttggtgcca tctttattga accatcaagc acaactgaga aagcattttt ttgatatatg
     5401 acttcttcta ttgtgtctgg aaatctaggg tccaatactc tatttattac tacagctgct
     5461 acagcaacct gtccctcata gctttctcct ctagcttctc ctgcaaccaa cttagataat
     5521 aacatcaagt cattatcaga taaatttatt acttctttct ttgctatatt tttttcttgt
     5581 ttgtttactt tcttaatatt ttctgttggt tcatatgcaa atacctcttt aataggcata
     5641 aatataaaag tcaaagctaa tagtgtagcc atcagtttac tccacctgtc catgattaaa
     5701 accactcctt acattcttat ttttaaaatt ttttctctaa atccttgact acttcaagta
     5761 ctttaaactt aatttttact ctacttgttt tttttggtgt atcttgtttt attaacttat
     5821 attctttatc agttaaaact tccttcaact tatcatctgt agatttatct atcactccat
     5881 tagattcatc aacaaaacat agtggaggga acataacaca ccaccagttt tttccttcac
     5941 ctttcccaat aataatcttt aatgctttat attcaccagc tggaagaact atattagaat
     6001 attgtttagt agggaaattg ctatattgta tacctacctt tacactgtaa ttatatccat
     6061 ttttaagaat aatattttta cttatttctt ccagactact atattccttt tttataatag
     6121 cttcactttc ttttatactt tttgaatttt gaagctttgg ctgtaaatat tttattactt
     6181 catctctaac ttttagttta agttcttggt cttcatctgt attgctgttt gctataacat
     6241 gaaatcttat taatttatct ttataatctt ttgatatatt gtctactttt tttacctcac
     6301 catttattac tattgtcatt attgatatga ctgatataag acttaatatt aatagtccca
     6361 atctaatctt aatttttctc attgaaaatt ccccctcaag ataaatttat atcatcaatt
     6421 atgtccaatt aaatttaatt tattcataat attttttaat actaataaaa aaagctatct
     6481 taaaatgatt acaatcattt taaaatagct ttttaaacct tatattttat aagtctttac
     6541 taatcatagc cttatctatc ataaactgct ctgctttttc tatatgctct gtagcatact
     6601 tcttggatag ctcactatca cctttaatta tagcatcaag taaatattta tgctcttcaa
     6661 caatattatg tgatgtatca taagcagaca tataggtaac tctaaatcta tatacttgtt
     6721 cccacaatga gtttattaat tggattaatt ttttattatt agttgcttta aatatacatt
     6781 catgaaattc tacatcaagt tttaataacg catctacatc ggattccaaa gtgctttttt
     6841 taaattcttc tgatatatct ttaagctttt taatatcttc atcactaatt ctttcagcgg
     6901 caagatatgc tgctaagcct tctaggcttg ctcttacttc taatacatcc ataatatcct
     6961 ttaatgacat atttgccaca tacgcacctt ttctaggcaa cataacgact aaaccttcta
     7021 attcaagctt tcttattgcc tctctcactg gagtcctact tactcctaat tgttctgcta
     7081 gttgcacttc cattagcctt tgaccaggct ttaattttcc ttctaagatt gcttctctta
     7141 aattctcaaa gactacatct cttaatggct tgtaattatc caagttcagt ttagttaaat
     7201 tctccacaaa tttcaacccc tttttgactg ctattaacaa catacacctg tttatatttt
     7261 tttaataatt ctttttttcc aattagagca tcttctttat ttttaaatag tccaaaaact
     7321 gtaggaccac ttccactcat catagaacct aaagcattat ttttcatcat tacttgtttt
     7381 atatcactta tttctttgtg tttacctata gtaacatttt caagaatatt taccatgctt
     7441 tctgatacag cttttatatc ttcagatttt aaacactcta tcaaatattt attatttggt
     7501 ctttttttaa tattattcaa atctaatcct tgataaactt ctttagtaga aacgaataag
     7561 tctggtttgc atatcaatat atttgtatca catggtaaac ctttgatatt tgttagcttt
     7621 tctccaatac cttgagctaa ggcaggtctt cctgatatac aaaaaggaac atctgcgccc
     7681 aaattaagtc ctatttcttt taattcatct tcagataatc taagttccca taggtgattt
     7741 aaacctacta atactgcggc tgcattagaa cttcctccag ccatccctgc tgctacagga
     7801 atattttttt ctatgaatat ttctacacct ttttttatat aaaatttatt tttaagtatt
     7861 ttggcagctt tataaacaat attatcttca tctaaaggaa tatctaaact agtgcttttt
     7921 acctttattt catcttcgtc taattctttt atctttacta tatcgtataa atctatcgtt
     7981 tgcattatca tttcaacaaa atgataaccg tcttgccttt ttcccaagac atcaatggat
     8041 aaatttattt ttgctctact ctttagtctt ataaaatcca ttctatcctc cttgtttaaa
     8101 gaataatgca tcactaacaa taatattata ctacaaaaaa taaaataata gggtattata
     8161 ataccctatt atacttaatt actttaaatt ttttaatatt aatctactaa tacgactttt
     8221 ttttctaaaa taagacactt cccaggctta attggttcat caagttttat atcattaagt
     8281 tctgcaattt cattttctgt tgtattgtat ttttttgcta tattccagaa agtatctcct
     8341 tctttgcata tatatacgat tatgctagga gctttagata aatcatatac accttggtct
     8401 tctcccttga taataaaatt ttctgctttc ttatctaatg cttcagtaaa tctcttaatt
     8461 ttgataatca agtctatctg gtccctattc aaatcaactt cgactttatc aatacatgct
     8521 gtattaaaca cagatgctgt atcagttaga ttatccattt ctatatcatg ttcaaatgga
     8581 atttcctcac taattttata aaccaatttt aaaccttcaa ctgggacaaa taaaatttca
     8641 acttttatga ttccttgtat aatgctttta tttccttcaa tataactatt ttctatagac
     8701 atggttggac aaacgcttac aatatctttt atttgtatat tatcattatc atttcttatg
     8761 ccttctctaa ccataaatgt ttcttcacta cgtcttaaag tcttatttaa ttgaataggt
     8821 ttgtggtcaa attttattat cttttgaggc gaatatgcat cttgtaatac ttctcttgta
     8881 acttcatctg taacttttac cttacaacaa gccatacaat ctatttctaa aagacctgtg
     8941 ttgctttcac tattttgctt gaaaatatga ttaaaatctg acatagacaa caatacttct
     9001 tcagtcatac catcactaac tccagggact tctacaaatt gagtaaactc aatacctact
     9061 ctatccaact ctacaagttc accctcatat gtacaagcta acggatttat ttctaatact
     9121 ccaccaataa taactttatt gtcagtcact ctactttctt ttactttaac acatggattt
     9181 aaacttatta tagattgaat ttcctctgtg tttattgtta tagtatctct tatagaactt
     9241 tctgcttttt ctattccaac tatatcttga aaacaaattt cctttctgtg cttttgtatg
     9301 cattccactt gcgccacatc ttttacaatg tctaaccttt gcttttcaaa caaacttccc
     9361 cttatattca taagagctcc tactttaatc tttctctcat tcattatagt acaatccata
     9421 tgctctactt ctgaaaataa catgtattcc atatcttgaa taacattgtc tttttctatt
     9481 acttcgttta tatctacttt tccgtctaca ttagaaatag tatttttatc atctgcaata
     9541 tagattacat tataattaaa actacctctg caaagtatct tcccatctgc catttcaatt
     9601 tttttaagtg atatgtatcc ttctgttttt acaatttcat atacatctaa ttttttgtct
     9661 ggcacaactg cctccgcttc aatgaatgtt tgaaattttc caaagtctat tctattgtca
     9721 actttaatta catctttaat taattccata gtctacctcc ttaagtatta taaaaatatc
     9781 acactatatt tatatttaag gagcaaaaaa aaaattacac ctatttttaa ataggtataa
     9841 ttttaactta tttgtaattt atcttgatct ctaaaaactt gcaactttac attagatgtt
     9901 aataaatctg agtaactata agatactcta ggatatccat tactttcatc atctaacttt
     9961 ataacaaaca cgcttggata aactttttct agtataccct cttttgtaat aatctgcttt
    10021 cttcctttat tagctttcaa caatattttc tttcctatat gtctctctaa gcttactctt
    10081 atcttatcta gagtttgaac agtagccaca atatcacctt ctttaacgtt tatatgttta
    10141 cataatatca catataaaca tttttgtcaa atgtttaaac taattatttt atatattttt
    10201 tgcaaaaatg tcaatactat ttttgaagat atacagtata attatgttaa tttttgctaa
    10261 tgtaatattg tttttggcat tgcccatcta aattttcctc ttgtttcaga accaccaatt
    10321 cccttaacac ctgtaatagt ctgctctata acctcatcaa taggaactac tacatcaatt
    10381 cttgaacaag caattttctc cactataaaa acagggtcta atgcatgtat cattattctt
    10441 ccaggcgaac ttgcatagtt tgctcctgca cttattattt tttcataatt tgattggcat
    10501 gctcctgcga atatgacaag attatccaaa tttggttgcc atttacgtgc ttcttttact
    10561 gttttaacaa aatttgaaga gtttctatag ttactcatat cttctatatc tcctcttttt
    10621 attgtcattg catcatgacc tgttattact agtatatccg gattatattt ttctagtagt
    10681 gctcttactt ctttatactg attttgctct gaaacagcta cacctactgc aggtattcca
    10741 agttgagcat atacatccaa gcaaattttt aaatattctt tatctccatc tatctgtagt
    10801 acttttccag gcattccata tgtgttagga tttaattgaa gttttggaac tgctctagtc
    10861 attctttttt gtctttcttt agcttttctt actgacttat ataataaatt ttctacattt
    10921 ttatctatta atatatcttt tatatcaggt gcttttacta attctaggtc atcaatataa
    10981 gcatctgcta ttactctaaa tgcaattccc tttagtatag ctattttctc attattttca
    11041 tctacaccaa aagagactat tttaaatact atatctttgt tatgtgactt tctagctaca
    11101 atgtctccta ccttcatagt taaacctcca ataaattttt atcttagttt atactatgtt
    11161 cgatataaaa ctttgtttcc taatgtcaaa aaaacatttc ttataatact tcttttatca
    11221 ctcttaaagc atttttatag tatatctttt ctatatcatc ttcactaaac ccttcttttt
    11281 ttagaggttc atacagttta cccatctgag atatatcttc aatctcaact tttgaatcta
    11341 ttccatcaaa atctgaccct agagatacaa catctattcc tccaacattt acaatatgtt
    11401 ttatgtgtct aaccatatct tcaagtttac tttcactttt atcacttaaa aataagtgga
    11461 agaagtttat acctgtaact ccacctttat ttgctaatac tttaatcata tcatcagtta
    11521 aatttctaga atgattcatc attgctcttg aattagaatg tgtagcaatt attggtttag
    11581 aagaaatctt tgcaatttca taaaatccac catctgaaat atgagaaaca tcaaccaaca
    11641 ttcccaattc attcatctta tgtaccactt ctcttccaaa atcagtaagc cctttttctc
    11701 tatattctac tttattatga ggaaagctca attcattcac atgattccaa cttattgtca
    11761 tcattctaac ccctaagtcg taaaatttct ttaaattttc taatttacct tcaagtactg
    11821 caccttcctc aatagataaa agagctgtaa gtttaccttc tgattggttt ttcataattt
    11881 cttcatagtt agtagctaaa gctatttcat cactattttt tttcatttcc tcatgaaatt
    11941 tatttgccat actcatgcaa taatcaaaag gatgttttac ttcttcagta tcaacaaaaa
    12001 gagcaaatgt ttgagctaat gaatccccct ttttcaatct gtctatatca acagaatatt
    12061 tattagactt aagttcactt gtttctacat tttccattaa ttttgctata gtatcacaat
    12121 gtaaatcaag aaatttcata tttatacccc ctgtatattt tatatttata tctaaaaata
    12181 gaaatagttg tccttataga caactattga caactatttt ttaatattct ttttaatatt
    12241 acttaatctt attcttcttt taagttaggt attacttttt taacttcttc aatttgatgc
    12301 catgttttat ctatattatt ttcactaata tattttttta aatcagttaa agatgtaaaa
    12361 tctttaactg ataaattgtc atcatcatct gcaaatctta aaataaacat gagactccct
    12421 tactttccac aacatttctt atattttttt ccgcttccac atgggcatga atcatttctt
    12481 cctatttttg cttctttaac tattgtttta gacttcttat aagcctttat tatttctttt
    12541 ctttggtctg ctggtagtat atcttcccaa ccttgtaaat tatatagcca atgagcctca
    12601 acagttacca tattgttata taatttttcc caatctattt ttatagaaac ttctttatca
    12661 gcttctactt cttctaaaac tatatcttct tttaagcttt cacttattcc atctatgaat
    12721 cccatgaaaa attcattagt agttccaaat ttttcagcca attcacttac tttaccttca
    12781 acaacatcca ttttattatt taatatttca ttgtatattc cagcttcaac tttcaaatat
    12841 tcttcccaga attttacttc tgcttcttga ctttcatgat tatcacttaa atttctccat
    12901 tcagtatata aactcatact ttttcctcct aagtatacct atatttttat agtttttata
    12961 gtataaaatc ttttagttag attggaagtt tcttcttttt gattatacca tattctattt
    13021 aaagttaact cttatattat atcataatta aacaattctt ttaatacttt tatagcatac
    13081 tcttggttta taaaataagg gcttgtcgca attgttatta ctttggcttt tttaattaag
    13141 ttttttatct tgtaaaattt gagttcaaat ggtatatagt ccatatcact tgaaaatata
    13201 tccaaatcta tatcaagaac aaactctgat tctacttcta aatcaaatcc atatagacta
    13261 tctattatta ttaattctga aaatatgtta tattttaaag caggttttat aaaacttccc
    13321 acatttaaaa cttcatttgt atatctaaat acatctttta gattatttac atctacatca
    13381 tagttctcag gttctctagt atccttatgt tggtctacat gcactaactt gcatcctttt
    13441 gtaaactcac cctttaacaa agattttgcc caaaaataaa atgcatggtt atgattgtca
    13501 aacacatata tctttttatt tcccaggaca tattcgacca tattctccaa tccttttgct
    13561 tttatttcct tattttcatc tacttcatta aatactacct cattgcctaa ccttacatca
    13621 tttaatgtac catttattag ttttggaacg tatatgctct tattttctct tttatcatat
    13681 gaaaatacat tatttcctac tggtttttct atataaaatc cactatattc ataattttcc
    13741 ataaaacttt atttcctcca ttgttatata aatatataag ttataatttt aaaaattaaa
    13801 atgcacttat tgtaaatcat atcatctatt atagaaaaca tcttatataa ctaaatataa
    13861 tatcttctct caatatgtat tatacaataa gtgcaaaata tatttatata aaatatacta
    13921 cattcctata acaaaaccat cagcttcaat tggaaggtaa ctaactcttc tattcaactc
    13981 cacctttcca aaaaatacta cccaatatgg tctaaaaata ctttcaattt taactatttc
    14041 aatctctggg attcctccca taaacctatg agttaatctc atagccattt tatttgcctt
    14101 tgcttttact cttcgttcgt ccttatcact atactgcaca atattacaat ccacattttt
    14161 tacaacttca tatgtaggaa aatcttgtac tatactacca gaacctgtag aaccattcac
    14221 taaaattttt aattcattta tatcattaga atatctccta ttcttgaaaa tggatttcgt
    14281 tttgtgaatt ttaagtgtga taattttaaa ctctatataa tgaagtctaa cttcatgagc
    14341 atatttgttc tttaatatta gcttactaaa taaagaattt tttccacttt tagctatctc
    14401 tatagcttct tcttttgtaa ttcttatagg taacgctctt atatccataa aatcattctc
    14461 tttcttattt ttttcttgtc atttttaaaa ctctatatat ataatcaaaa aatattacaa
    14521 ttacaactgc cataactcca tatggaatta tttttccaac tataatatca tttctagtca
    14581 atataccata aaatataaat cctgttatta aaaagtacaa taatactaga aaaccgtcta
    14641 ttccaagcca ttttatttct aaatcttttt ttaccttaga cactaattta gacatagcag
    14701 tttctccttt tatagattat tttgtgtatt taaattttca ccgattctat taccaatcat
    14761 ccatgcaata aatgaaaaag gtatagctat cacaagaggc tctaactcaa agctctgaac
    14821 tggaaatatt ttaaaataac caaaagtcac tccaccaaca atcattccta aaataccacc
    14881 catagatgtt cttcttccat ttccaagaag cccacctact ataggtacaa atgccgaacc
    14941 catccaaata cttcctacaa atatacatgc atcatataca ttttcaaatc taaatgctac
    15001 gctaagcccc ataactgctg ctaaacatac acctattcta gtaaacttta ctaattgttt
    15061 ctgagataac tctttttttg taattcttcc atatatatca tttgctaatg tagccccagc
    15121 tgctagataa tagctatcca atgttgatat tatagaacct aaaattccta ttataaatat
    15181 tactcttatt ccttgtggta atgtatctaa aactaaagtt atatatccag cagctggagc
    15241 catttctgga taagtagctc ttactataag tcctgtcata gttaacacca tgtcaaaaca
    15301 tatccatagt gaaaaacagg ttaataaggc tcttcttcca accttaggag aatctgctgc
    15361 tgaaaatctt tgataaaaag ttgggtctga ataagctcca aaagaatata caattaacat
    15421 caatgctttc atagctgaca taccttttgt agggtgcata agctgtggtg catctttgag
    15481 tgcttctgta agaccttgaa accctcctat ttgttcaaat tgacctggca taaccatagt
    15541 taaaccaaat accatacaaa aaaacaatac catatctgta atggcaactc ccataagccc
    15601 accaagtact gtaagtacaa cgactactcc taccattatt aatcctatta attctttatc
    15661 tattttccat gctacgttac ctatagttcc catagcagtt atttctccaa gttgtgcaca
    15721 atatataaac atcaaaattg ccccaattat agcaacatgc ttgccataaa aactttccag
    15781 taaatcagga actgttatat tggttcttat atgaatcctt ggaccaaccc acaatgctaa
    15841 aggcattctc ccaatatgtg cagctattga ccaaactgcc catgcagata ttccatatat
    15901 agccgcatac tctacactac ctactgtacc aactcctcca taccatgatg caaccaaagt
    15961 tcctactatt aaggaccatg gtaaacttcg atttcctagg taataacttt ccatatcttt
    16021 tacatttcta gaaaaataat aacctattcc taataatata aatgcaaata taactataat
    16081 cccattttcc caaaatgtta acatatctaa tctccacatt cttatatttt aatttttaac
    16141 tttaatatct aaatatgaac aactctaccc atttccataa aaataggtag agttatataa
    16201 taattataag tatttaagta atcttgttat taagcaattt tattgtttaa acctttattt
    16261 tctcttttat ttattttatt taaagcataa gtagcaaata tccctataac tgcacatact
    16321 acagataaaa taaatatata tgtatatcct gtttgtccat atttatctaa ccaattacct
    16381 gcaatagtgt aataaaacgc atctggagaa aatcctataa atgaagcaaa tccaactact
    16441 gctcctgtag tttctaaaga tatattagac tctgaaacag atgcaaagta tatacctcta
    16501 aatccaaata aaattacact tagtattatc atgtttatta cagctatcca tataaatcct
    16561 gtactcttag gaataaccaa gtaagcagct gtacttactg tcattaatat aaatcctaca
    16621 aacattactc ttatagatga accaacttta tctgttataa caccagcaac tggagatgct
    16681 cccattttta taacataagt tcttattatt gataaagcag aaactagagt cattggcatt
    16741 acataaactt ctgataagta tggacttaaa taagttaaac ttgagaatac taaataagtt
    16801 gaaaatatta taagaccaat taaccaagcc tttggtattg taactacttt taacaatgac
    16861 ttaatactta atccttcatc tgatgctcca ctatcttttt tctcttccac taagaaaaat
    16921 agaagtatac cacatattat agatgctgca gaatataacc atactacata tttgaatcct
    16981 actgttattt cagcaaattt accaaagaaa taaagaccta taaaagaaat taatgtacct
    17041 gatacacctg ataacccttc ataaaaacca aatattcttc cttgctctga ctcacttcct
    17101 tgcattctaa caaccttaac agaggctgag aagaaggtta atattgtcgt aaatccccat
    17161 aatacaaata ttaaaagtat catactatat gatgggaatg tagagaaata aaatccaagt
    17221 atccctgaag caattaaaga aaaagttatt aattttctaa ctgagaatct atctgcaaac
    17281 catcctccta taaaataaga taccattcct actaatccat ataaactcaa taagttccct
    17341 atttgtgtat gacttaatgc taaagcttgt tgcattgggt cataaaatga tgatttcata
    17401 tatggtaaac tatacatagc tgttgttcca aatgccaata tgaatattat caaaaatctt
    17461 ttaacattat ttttgctttc cattgattct ctctccttaa aattttattg tttataatct
    17521 cattcatata attttatttt aatttttcta ttgcttgttt aaaatcattt attatatcat
    17581 ttgaagattc taatcctaca gctattctta ttagattttc cccattcatt tgaactaatg
    17641 tatgctcttc ccctaagctt gttgctattg ttgcaagctc taaagagttc attaatcttc
    17701 tactagcttg gtctcttgtc aatccactga taccatcctt aagtttaaat gaaactacgc
    17761 ctccacctaa accattcatt tgagttttac aaagctcata ttgaggatgt gattttaatg
    17821 ctggatactt taccaattcc acattctctt cttcctctaa aaactctgct actgctaatc
    17881 cattttcaca atgctttttc atcctcatat ctaacgtttt catacccctt aaaatcatcc
    17941 atgcactcat tggtggtaaa gatgctcctg taaaacaaca taaacttggc catctaacta
    18001 aatctattaa ttcttttgaa cctgctactg ctccccctaa agtatctcca tgtccattta
    18061 taaacttagt aagactatgt attactaaat ctgcacctaa atctattggt ctttgtacaa
    18121 ttggtgtccc aaaagtacta tcaactatag ctaagctttt attttcatga gcaatttctg
    18181 ctattgtttt tatatctact aagtctaatg atggatttaa aggactttct atataaacta
    18241 ttttagtatt tggtctcatt gcttctttta cattttgtgg atttactgca tctacaaatg
    18301 taacttcaac tccaaattta gataacaact cagacatgaa taatttagtg tgtgaaaata
    18361 ttccatctga actaactata tggtcaccac tttttaccaa tgctaatata gtagatgtta
    18421 ttgcccccat gcctgatgta ctagatatgg cactttctgc attttcaagc atagccaact
    18481 tagcttctaa ctctgtaaga gtaggattac actctcttgt atacacatat ccccatgtca
    18541 tacacaactc ttcaaaatgc tctactgtct ttgcagcaaa tgttgctgtt tgacatattg
    18601 gtgtagccaa tgcacctgtt gttgggtctg cactatgtcc ccaatgtatt atttttgtct
    18661 ctaaatcttg attaatattg ttactcattt tgatttcctc ccattttgac ttatttatac
    18721 aacgttttcc ctgccaaatt tcatcaattt agcttagtct taatacattt tttcaagtat
    18781 acttttttat ttgttataat ttctaaaaat atgacactta ttttgttttt ttacagttct
    18841 aattataaac tctatagtaa ctatagagtc aatatgtttt tgatattttt tttacaatgt
    18901 ttaaaatagt caatacaaaa ataggtatat cttagaataa ctaaagtcat cctaagatat
    18961 acctat
//
LOCUS       DAMJZE010000069        18936 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJZE010000069 DAMJZE010000000
VERSION     DAMJZE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.069"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(66..2090)
                     /locus_tag="PC541_002039"
     CDS             complement(66..2090)
                     /locus_tag="PC541_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839505.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            2480..3148
                     /locus_tag="PC541_002040"
     CDS             2480..3148
                     /locus_tag="PC541_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839506.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            3150..3635
                     /locus_tag="PC541_002041"
     CDS             3150..3635
                     /locus_tag="PC541_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2839507.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            3696..4817
                     /locus_tag="PC541_002042"
     CDS             3696..4817
                     /locus_tag="PC541_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2839508.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            5098..6288
                     /locus_tag="PC541_002043"
     CDS             5098..6288
                     /locus_tag="PC541_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2839509.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    6422..6677
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6736..7737
                     /locus_tag="PC541_002044"
     CDS             6736..7737
                     /locus_tag="PC541_002044"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2839510.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            7841..8230
                     /locus_tag="PC541_002045"
     CDS             7841..8230
                     /locus_tag="PC541_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2839511.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            8391..9599
                     /locus_tag="PC541_002046"
     CDS             8391..9599
                     /locus_tag="PC541_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2839512.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(9685..10761)
                     /locus_tag="PC541_002047"
     CDS             complement(9685..10761)
                     /locus_tag="PC541_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2839513.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            10990..11517
                     /gene="hpt"
                     /locus_tag="PC541_002048"
     CDS             10990..11517
                     /gene="hpt"
                     /locus_tag="PC541_002048"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2839514.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            11606..12538
                     /locus_tag="PC541_002049"
     CDS             11606..12538
                     /locus_tag="PC541_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2839515.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            12600..13826
                     /locus_tag="PC541_002050"
     CDS             12600..13826
                     /locus_tag="PC541_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2839516.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            13910..14131
                     /locus_tag="PC541_002051"
     CDS             13910..14131
                     /locus_tag="PC541_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2839517.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            14320..14490
                     /locus_tag="PC541_002052"
     CDS             14320..14490
                     /locus_tag="PC541_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839518.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(14583..15044)
                     /locus_tag="PC541_002053"
     CDS             complement(14583..15044)
                     /locus_tag="PC541_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839519.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            15228..15695
                     /locus_tag="PC541_002054"
     CDS             15228..15695
                     /locus_tag="PC541_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839520.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            15738..17501
                     /locus_tag="PC541_002055"
     CDS             15738..17501
                     /locus_tag="PC541_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2839521.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            17509..18117
                     /locus_tag="PC541_002056"
     CDS             17509..18117
                     /locus_tag="PC541_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2839522.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            18189..18758
                     /locus_tag="PC541_002057"
     CDS             18189..18758
                     /locus_tag="PC541_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2839523.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         7261 a         1994 c         3199 g         6482 t
ORIGIN      
        1 atagaaaaat aaaaaattat aattatatat aaattttgat aaagtaaaga tgaattacta
       61 taggtttaaa ttatattatt ttctattatt tttatgcctt tctcagttat tgatgagcct
      121 tttcttccct tactagaaat taagtatcca ttgcgtgtca aatattctag catatttctt
      181 acttcgtatt ctgtgacatt atattccata aatttcatta actttgtcct ccccattggt
      241 ttcatgtgca atgattttag gatagaaatg catatgtttt tgtcatattg gtttaaatca
      301 ttgaatatgg acatattact tgacatatga ttattattta attttgaaga tatgttttga
      361 ggtaaatcat ttattgtaag ataattggaa cccatgtaag ccatatattc aacacaattt
      421 ctcagttctc taatattccc catccaagtg tatgaattta aagcttctaa aagactctta
      481 tcgagtttta tgtttttata gggaagttct tttaaaaatg attctaaaag aagaggtata
      541 tcttctaatc tctttctaag aggaggtaaa tctaaagtga acatacttat cctataaaat
      601 aaatccatcc taaatttatt ttctataatt aatttctcta aatctttatt tgtggcagct
      661 aatattctca catcaattgg aataatactt gtaccaccaa tttttctaat ttctttttct
      721 tgtaagactc ttaaaagttt aacctgtaaa tcatgtggca tatctcctat ctcatctaaa
      781 aatatagtgc cagtatgagc taattcgaat aatccttttt ttccaccttt ttttgctcca
      841 gtaaaagaac catcttcata tccaaatagt tcactttcaa gtaattcact tggtaatgaa
      901 gcacaattta ttgctacaaa aggtttattt tttctatttg aatgattgtg tattgactga
      961 gtaaaggctt ctttacctgt tcctgtttca cctgttatta atataggatt atctgtaaga
     1021 gccatttttt ttgccttttt aatacaatct tttataatag aactatttcc aataatatta
     1081 ttaaatttat atttagctac ataacctctt ttgacagaat cacttcttat tttttgttct
     1141 aatatttcta tattgtttac atcttttaat gtaattaagc aaccctctat atttttataa
     1201 agataaaatg gttttttaga aagtatgaat tttttatcta tttcgtctat gtatataatc
     1261 atattatcta tatttaagtt ctttgtaatt ttatctaaga aactctttgg catatattcc
     1321 attaaggaag gtgattttat aagtttttca tctagttcca acatattaag taaagactta
     1381 tttacaaaag taaccttatt aaaggtattg aaaaatatta gaccatcacc tatttcatca
     1441 atggtcatgt aaagtatagt ttttaattta gatatattat ctaatgatgt atttaagaaa
     1501 tcatgactta gtgtctcctt ggacaatttg tataattttt ctataaattt ctcatttttt
     1561 agttttaata ctactaataa tgacatatat gtatctaatg agatttttct ccaacctaaa
     1621 tcaacaatat tttttatgta ttttggtata aatctaatct gacctggtgt tatagctgta
     1681 tctaaatcat atatttcatc acaatctgga tatgtcggta caaaatctat atgatttata
     1741 ccaattttat atagataagt agccatctca aatgctgaag ttttattggt tgtcataagt
     1801 aacgattttg tacctggagg cagtttcatc attttttcat atccaagttt tgaaagtgct
     1861 ttcgttccat gaataatttc aatatcttcc ttaaaatgtt ttattaaaaa attactagtt
     1921 aaaggtgata gagaaagcac tacatctccc tctaaatttt cttttattcc atcaagtata
     1981 ttttgtgttt ctatgattat ttcgtctcca aaaaagtttg ttaattcttc tttgtagtgg
     2041 tcactattca atttatcata tgttataagg attaacttct ttttatccat tttatttctc
     2101 ctaacaaaat agtatttatt tttaaagtat ttaaagtata aggcaagtta tattatacaa
     2161 caaatagtag tataaattta tatatttgat taaataatag gtttttttag gattttatcc
     2221 tataaaaacc tattttgaaa ttatttttgc aagataagga aaatagcttt ttatattgta
     2281 atgtatattt taaataatta ataaaaaaat attatatttt taaacttgaa aagtaagtaa
     2341 ttataagcta tttaatcact ggaataaatt tacaataaaa aaataaatac aaaggcttta
     2401 tcattaaggt attggcataa gttttgctta ttaaacaagt atactaataa aattcattta
     2461 aacaggaggg agaatttata tgagtaaatt agaatcaaca acagaaaaag ttgaggtaaa
     2521 atggtatggg tatgtagcat taatattggg agcattattt ttttcaggaa tatttaaaga
     2581 tgcaccagga ccattaaaag tattggattt taacaatgta ttaggttctt ttggaacatt
     2641 gggaacaatt aatgatggtg taggtacatt agctgcaaac tttagaggtg atggaggtac
     2701 tggccctcga gatggatggc tatatgcatt gacattaata ccatcagtta tgtttgcact
     2761 tggagtagtt agagttatag accacttaga tggaatgaag gcagctcaaa agcttcttag
     2821 tccactatta aagccattat tagggttacc tggatttgca ggactaactt tgatagctag
     2881 tttacagtct acagatgcag ctgcatctat gactaaagaa cttaaagatg atgggtatat
     2941 agatgaaaaa caaaaagctg tattttgtgc atttcaattt tcaggtgcgt ctgcaattac
     3001 gaatttcttt gcttctggag cagcactatt tccatttata ggagatgtac caatatttat
     3061 accacttgct ttaatattaa ttatgaagtt tgttggggcg aacttactta gattatattt
     3121 aaataaattt gacaaggggg aagcttaaca tgagtgaggt agcaaaaaaa gaagtaaaag
     3181 ttaaaaaagg aaatgtgata gatgcgttta ttggaggagc tagaaatgga ttccaaataa
     3241 gtacaaattc aatggctcca aatgtcatct tcggttttgc tataatttct gtgttcaact
     3301 taacaggtct tttggatgta atagggacaa tatttacacc tgtaatgagt atctttggac
     3361 ttccaggtgt tgcagcaaca gctataatga caatatttat atcaatggga ggagcatctg
     3421 gagtaatcgc aggtttattt acagcaggtc aaataagtca agcacatgtt gcaattctat
     3481 tgccaatgtt attgctttca ggagcgcaga tacaatttat ggggagactt ttaggaacag
     3541 ctgagttaaa aactaaatat tatccacata tgtttgttat agcaacttta aatggttgta
     3601 ttgcaatgct tataatgaat tatttcgtag tataggtaat tataataaaa atttaatata
     3661 aatggaaagt ttaaatttta aggaggagct agattatggg aactaacaaa gattatattg
     3721 agtatgtaca taaagtgtat gattatctac acactatacc agaaaaagga tttgaagaat
     3781 ataagacatc agagtttgtt ttaaaagagc ttgaaaaaat ggggtttgaa aatattaata
     3841 aaggagttgc aggaactggt attatagtaa ctatagattc taaaaaagaa ggaccagtat
     3901 ttgctataag agcagatatg gatgcattgg aatttatcat agatggagaa aagaaaatga
     3961 tacatgcttg tggtcatgat ggtcatactt ctatgctttt aacagctgca aagttaataa
     4021 aagagagaaa tttagtaaaa agaggtaagt taaaaatact ttttcaacca ggtgaagaaa
     4081 aattatatgg agcattaaga gtaattgatt ctggtttgtt ggatgatgta gaagaaatgg
     4141 tagggatgca tttaagacct ggacaagatt gtaggttggg aaaggcgata cctgcaatgt
     4201 accattgtgc tagctgtata atggaattta cgattaaagg tatcacttgt catggttcta
     4261 aaccacattt aggagtaaat gcagtagatg ctgtctgttt agcagtgaat gcaataaatg
     4321 caataagagt aaatccagct ataccaaatt ctataaaagt aacaaatata tcagttaagg
     4381 gagaaactta taataatata ccagatgaag catttatggc tttagatata agggcagaaa
     4441 gtaatgatat aatagaagaa ttaaaagaaa aagcaataca tgctgtaaaa actgttgtaa
     4501 gttctataaa tgcagaagct gtactaattt ccaaagatgg agtaccagca gcagaatttg
     4561 atgaagaact tgtaaacttg gctgaagaaa ctataaaaga tgtattggga gctgatgcat
     4621 ctataggtat atttaaaaat tctggtggag aagacttcca ttattataca caaaaactaa
     4681 aatgtaaaac tacatatatt ggattaggag ctgatgctac accaggattt cataatccaa
     4741 atgtaacatt tgatacgaaa gctttagaat atggtgtaga tatatggtgt agattggttg
     4801 aaaagagatt gggataacat ccaaactaat aattctacat ttaaaattat aattatagaa
     4861 gttatagaaa aagattgctt atgttaatta gaaaataatt aatatgagca atcttatttt
     4921 gatataatga aactgatttt ggaatattga aaaaatatga tggaaaagta attatatttt
     4981 aaatctattg gacaaaaaag tgatattatt ataatattaa gaattgtaaa ataataccaa
     5041 agtgcatcta gtaatagact taacaattat aaaaatttta ttcttaggag ggaaaaaatg
     5101 agtaagtata attttgacag agtaatagat agagtaggaa cagattgtgt aaaatgggat
     5161 tttagaacta attgttcaac aaaagcacaa aaagatggtt tgcctttttg gattgctgat
     5221 atggattttg aatgtgcaga accaataata gaagcattac gtaaaaaagt tgaacataaa
     5281 atatttggat atagttcaaa tgatagtgat aaatacttta atgcagtttg tgattggtat
     5341 aaaaggagat ttaactggga aataagtaga aaagatatta tttttagtcc tggtgttgta
     5401 cctgctgttg ctattttagt aagaatatta actaattcta atgaaggtgt aatagtccaa
     5461 aaaccagtgt attacccatt tgaagctaag ataaagagca ataataggga cgttgtaaac
     5521 aatcctctga tatatgagaa tggtacttat aggatggatt atgatgattt agaagaaaaa
     5581 gctaagtgta gcaacaataa agtactaata ctttgtagtc ctcacaaccc tgttggaaga
     5641 gtttggagag aagatgaatt aaaaaaggtt gtagaaatat gtaaaaaata tgacctatgg
     5701 ataatagctg atgagataca ttctgattta attagaaaag gatttaaaca tactccttta
     5761 cagtcattat gcccagagta caaggataaa atagttacat gtatagctcc aagtaaaaca
     5821 tttaatctgg caggaatgca gttgtctaat ataatagtga ataatgatga actaaaaaag
     5881 aaatatcaag aagaggttac agctgttgga gttgcaactt caccaaatcc ctttgctatt
     5941 gtagctacaa tagcagcata taatgaaagt gaagactggc ttaatgaatt aaatgattac
     6001 ttagataata atatacaatt tattgatgaa tacttaaaag aaaacctacc aaaagtaaaa
     6061 cttgtttatc cagaaggaac atatctagca tggttagatt ttactgctta tggattaaat
     6121 gaagtagaac ttgaagattt aatgtttaaa aaagctaatg tattatttga tgaaggttat
     6181 atatttggaa aagaaggaat aggatttgaa agaataaatg tagcttgtcc acagtcttta
     6241 ttaaaagaat gtatggacag attaaagact atatttgaaa atttataaag tataggtaaa
     6301 aatttataaa gtgtttaaaa ataaaaaaat aataaacttg acaaatttta actaatcact
     6361 tatcatacta ttatgaataa atagaaaaca taagagagct aaattttatt gaattaaact
     6421 cttaaagact ttgaaaggaa gagtagttat agaatgtaat taaagcgagt tggtgatggt
     6481 gtgagaccaa tatgaagtct atagtgaaga acatcctgga gttgctaacc gaaattttaa
     6541 aagagtaggc ttagtcggaa ccaaaccgtt atcatattgg ggtgtatcga gtaatatctg
     6601 tacattgtga gctgggtttg aatttatttc aaactaatca gggtggtacc gcggaaaagc
     6661 aacctttcgt cccttttata tagggatgaa gggttttttt aatggaaaaa tatataaata
     6721 tagggagtga atgatatgag cacaataata ccaaaggatt ataaaagtag tttgaacgta
     6781 attgatacac aaatagctat aaaaaaatta aaagattttt ttgaaatgag actaagtaat
     6841 gaattaaatc ttataagagt ttcttctccg ttatttgtat tgccagaaac aggtgcaaat
     6901 gataatttaa atggagaaaa ggctgtaagc tttgatatac catttatgaa taaaaatgca
     6961 gaaattgttc aatcacttgc aaaatggaaa agattagccc ttaaaaaata tgggtttgaa
     7021 gttggtagtg gtttatatac agatatgaat gcaataagaa aaaatgaaga attagacaat
     7081 atacattctc tttatgtaga ccaatgggat tgggaattgg ttatagataa aagtagtaga
     7141 aatgaaaaga ctctaaaaga tgttgtaaaa agattgtatg gagtatttaa agatactgaa
     7201 atttttgtat gtagtatgta tgaaggtata aaagaaatat taccagaaga aataactttt
     7261 ataacatcac aagagttaga agatatgtat ccagaattat caccaaagga aagagaagat
     7321 aaaattgtaa aagagaagaa agcagtattt ataactcaaa taggaaaaac attaatatca
     7381 ggagaaaaac atgatggaag aagtcctgat tatgatgatt gggaattgaa tggggatata
     7441 ttattttaca atcctgtttt agattcagca ttagaattat cttcaatggg aattagagtt
     7501 gatgaagaat ctcttgatag acaattgaaa cttgctggat gtgaagagag aaaagagttt
     7561 gactatcata agatgctttt aaatggcgaa cttccgtata caattggtgg aggaattggt
     7621 cagtcaagaa tatgtatgta tttcttacaa aaagctcata taggagaagt acaagttgga
     7681 gtatggccac aggatatgat tcaaaattgt agtagtgcag gaattgaatt attataattt
     7741 aaatataaac taattgataa aatttatatc taatatgttc atcaaattaa ttatatcggt
     7801 tataatgtag aataacctat aattataaga ggtgataaat atggagtttt taaaaataat
     7861 cattaatata gttttagata ttttaaaaaa aattttggtc agatttaaaa atgctaaatt
     7921 tggtttggtt tttgtatttg atttacttaa actacctgat tttatgacag ataaaagaat
     7981 caatatagtt gataaaataa aggtaatttc tgtattaatt tttactatat cctactttgt
     8041 atctggggtt gatataattc cagagatgat tgcaggggca tttggtttta tagatgatgc
     8101 tatagtttta atttggagta taggtatagt gaatgaagaa attaataagt atagagttat
     8161 cgcaaaaaaa gataaacatt caaatataat tgaaaatgta gaattttcta taaaagatga
     8221 agaagaataa caatttatat atgttattct tttttttatt aacaaaaaat ggttaaattg
     8281 ttgacggaga ggtaaaactc tattatcata ataagtggat atccaatatt taactaagta
     8341 ttcaatgtgc aagaatgctt aatttaaaaa agttatggag ggtaaatatt atgaagaaaa
     8401 aaataggttt agtgcccaaa ctaatcatcg gtatcatagt cggtatttta atcggaagtt
     8461 atgcaccaga aataatagta caaatattag taacagttag tactttattc tcagcattcc
     8521 taaaatttgt tataccattt ataattatag gttttgtaac agcaggtata gctgatttag
     8581 caacaggtgc aggaaagtta ttggggataa ctacgggaat agcatatggt tctaccttaa
     8641 tagcaggatt attatcattt gcagtatcta cattaatctt cccaaatttt attgatgcaa
     8701 gtgttgcaag tcaaattgga gacccagagg caggaatgtt agctccaata ttcacaattc
     8761 cattagagcc tatggtagat gtaacagctg caatcgtatt tgcatttgta atgggacttg
     8821 gaatatcagc tttaagaaat catggaaaag gagagacttt atttaatttc ttccaagaat
     8881 ttcaaggaat cgtaacgaaa acgttgtcaa cagtgattat acctttatta cctttataca
     8941 tagcaggaac ttttgcaaat ataactatag caggggaagt atggacaata ttaggggtat
     9001 tctggaaagt atacttagta gtaatacctt tacattttgt atatatggca tgtcaattca
     9061 cagcagcagg tgtattttca ggaaaaaatc cagtaagaat gttaaaaaat caagtacctg
     9121 gatatttaac agctatagga actcaatctt cagcagcaac tataccagtt aatgttggtt
     9181 gtgcagaaaa aaatggagta agtaagcaaa taagagaatt tgttgtacct ctttgtgcaa
     9241 caatacattt atcaggtagt atgatatcaa taacaggatt tacaactgca gttttaatga
     9301 tgaatggtat gccacatgga ataggtgtat tattccctta tattgcaatg ttaggtatag
     9361 caatggttgc agcaccagga gcaccaggtg gggctataat gagtgcactt cctttcctac
     9421 caatggttgg aataccatca gatggtgggc ttgcaagttt aatgatagct ttatacttaa
     9481 ctcaagacag ttttggtaca gcatgtaatg tatcaggaga taatgcaata gcagtagtag
     9541 tagataaaat aaatgatgga atgagtaaaa aaggaaataa agaagagcaa agtgcttagt
     9601 tttttaataa tcaaaaatga gttatctcaa atagagatag ctcatttttt atggtataaa
     9661 aaattataaa ataaagtgat ttttttattt aaaaattgct ttaagagatg atattaataa
     9721 tttattttca ttaggtttta atatacatac tctaaagaaa tactcatcta gaccttcaaa
     9781 tgaacaacaa tctcttatta ttattttttg tggaagcagt tgctctcgaa gagatttagc
     9841 agtcaattcc tttgtcttga ttttacacaa tatgaaatta cctttagtat tgtatatatc
     9901 taagcttttt atattcttaa gctctttaag caggtaatct ctttcagtgt tcattaagga
     9961 aatagtattt gatataaaat ctaaatcact aaacatgatt tcacccattt tagaagctat
    10021 tatgtttata ttccataaat ctagattttt atttatttca tttttgacat tagtgttaga
    10081 tataagacca tatccaagcc ttattccagg cgttgaaaaa aattttgatg tacctcttat
    10141 aacgaataaa ttagaataat catcaactaa ttgagtacaa gagtatgtat ctgtatcagt
    10201 aaactctaca taagtctcat caatcattaa aaatgagtct gtatttttta aaatttctct
    10261 cacttctact ttagtaaatg caaatccagt tggattacta ggattacaga ttactaccaa
    10321 atcataatct ttagcattga tagtttttat aagattttct aaatttatat gaaaattatc
    10381 ttcttctttt gcaaagtatt tttctataga acaattaatt ttagataact ctttttcata
    10441 ctctgaatag gctggagaga gtaaaagagc ctgtttaggg ttaattgtat gtataaatga
    10501 tgaaattagt tctgtagctc cactgccaag tactatatta tctatagaac atttgcagta
    10561 attggatata gaagttttta agtctatgta atcagggtca ggatacatag ataccatatc
    10621 tatattgttt acaatgtatt gtttggctaa acttgatgta ccaaaaggat ttatatttga
    10681 gctaaaatcc ataaattctt cctttgaaaa gccatactta ctagataaac tatataaatt
    10741 agctccatgg tttgtgctca taaaagtacc tccaaaaata aaattatata atcgataaaa
    10801 ataactgatt aatatatgta catatagatt ataatacaaa ggaatcgtta ttccaaattt
    10861 ttaatgtaaa aagcaaaaaa gtatggataa gctgttaatt taggtttaaa aatgatgaaa
    10921 aagattttaa ttgatatttt ggtatgctat aatatataag ttaatagaag gaaagcggga
    10981 ggatacaaaa tgtacaaagt tacaggaaaa atgttgacag aagaacaaat aaaagaaaaa
    11041 gtttatgaat taggaaaaaa gatagaagaa gactttaaag gcgaagattt actagtggta
    11101 ggtatactta aaggagctag tgtatttgtt tcagatttaa taagatgtat agatttggat
    11161 gtaaatatag attttatgag tgttacaagt tatggaaact caacagagtc ttctggaaca
    11221 gttaaaatat taaaagattt agatgtagat attgaaggaa agaatgtgtt gattgtagag
    11281 gatataatag attctggttt aacattaagt aatctagttg cagctttaaa aactagaaat
    11341 ccaaaatctt taaaactatg tactctactt gataaacctc agagaagaaa agctaatata
    11401 cctgtagact atgttggatt tgttatagaa gacaaattta ttgttggtta tggaatagac
    11461 tatgctgaaa agtatagaaa tttaccttat ataggtatag ttgaggatgt agaatagatt
    11521 taaatgtgaa tcaaaagcac taatttctaa gggagttagt gctttttagt taattatata
    11581 aaattttagt aaaggagaca tctatatgga tattcagtta aatatattat tatttttcca
    11641 aaatttaaga aatccaattt tgaactttat atttcttgtc tttacaataa gcacagaagc
    11701 accgttatta atattaatta caacaattat atactggtgt ataaataaaa aatgtggaca
    11761 gaaaatactg tttgctataa ttggaaattt tgttgtaaat ttaggcatca aagagtttgt
    11821 aaaagctccc agaccaatag gaataaaagg tcttgaatca ttgagagttt ctactgctgg
    11881 tggttattct tttccaagtg ctcatactca aactgcaaca acattttggg ttagtattat
    11941 gactatattt aaaaagagat atcttcattt aataggtgca gttatggttt taggagtagg
    12001 attgtctagg ctgtatttag ctgttcattg gccaatagat gtgatagcgg gttggatatt
    12061 aggaatattt tttacagttg tatttgtaaa aatatttgac tatattgatg ataataaaaa
    12121 ttatatttta cttgtagttt tacttatacc atttataata gtggctatat ttctaaatag
    12181 ccctgaatat tttgaaaaat ttggtataat agtaggattt gtatttggat atatggttga
    12241 ggatagattt gttaaattta atacagataa taataacaaa aaaataaatt ttagcaataa
    12301 aaataagggg agtaaaaata taattttttc aagaatatgt aggtttttgg ttgggatagt
    12361 aacaatagga atgttatata ttggcattaa gttgtttatt tcaatgttaa tagtcacttt
    12421 aaatataagt gactctacta tgagtataat ttttatgaat tttattaaga atacagtagt
    12481 tgttttttat ggaatagcag gagctccagc attatttaaa cttttaaaat taaattgatt
    12541 ttaaggtata gagtcaatag tttaaattaa aaaaattaag ggtagaaaag agtgattatt
    12601 tgaagaaggt agtaataata ggtggtggac cagcagggat gatagccgca tcaactgcat
    12661 gtgagaaagg ctatgatgta acattaattg agaaaaatca taaattagga aaaaaacttg
    12721 caataacagg aaaaggtaga tgtaatatta ctaacgcttg tgaaatagag gaattaatag
    12781 aaaatgtacc tacaaatgga aaatttcttt atagtgcttt ttatactttt acaaatgatg
    12841 atgtaataag tatgtttaat aatcttggag taaagactaa aacagaaaga ggaaaaaggg
    12901 tttttccaga gagtgataaa gcatttgata ttgtaaatgc tcttgaaaga caacttaaaa
    12961 gtaagaaagt aaatatatta ttaaattcga aagtagaaaa aataatatct aaaaataata
    13021 aaattgaaaa agtaatttta aacgataaaa aagaaattaa gtgtgattca gttgtagtag
    13081 caactggtgg attaagttat ccacttactg gttctactgg tgatggatat aaatttgcaa
    13141 tttcacaggg gcacacaatt atagatacaa aaccatcttt aattggtata gaagtacaag
    13201 aaagttttac aaaagattta gaaaaattat cattgagaaa tgttgaaata agggtgttta
    13261 atagcaaaca aaagaaagtt tatagtgatt ttggagaact tgaatttact aaatttggtt
    13321 tagatggacc tataatcaag tctgcaagtt gtagaatgaa agatacaagg aaagaaaatt
    13381 acactatatt attagattta aaacctgcat tagatgagga aaaattggat aaaagagttc
    13441 aaaaagattt tcagaagtat acaaataaaa aatttgaaaa agcattagat gatttacttc
    13501 caaaaaaact aatacctata ataataaact taagtgaaat taatccaaat acggttgttc
    13561 accaaatttc tagagagcag agaaaaaact tagtccattt attaaaaaat ttaaagttta
    13621 cagtaaagag atacagacct attgaagaag ctataataac ttctggtggt gtaaaagtaa
    13681 acgaaataaa ttcaagtaca atggaatcaa aattagtaga aggtttattt tttgctggag
    13741 aagtaataga tatagatgca tatacaggtg gattcaattt gcaaatagct ttttctactg
    13801 gatatttggc aggttttaat tgctaaaaat tacctgaatt aaaaatgttt aaaatcacat
    13861 aatattatca gcaacacaaa ataaattaaa cagaaggaag gtaatcaaca tggcaagtaa
    13921 caataacaac aacagaacag tagtaccagg agcaaaagag gctttaaacc aaatgaaatt
    13981 agaaatagct aatgaacttg gtatgagtaa ttatcaacaa gttgataaag gaaaccttac
    14041 tgcaagagaa aatggttatg taggtggata catgactaaa aaattagttg aaatggctga
    14101 acaacaaatg gctggaaaaa gcaacagata gttataaata attatttata actattggct
    14161 attagtttag ctagagctat ctcaaatata gaaagtaatt tctaaattga gatagctttt
    14221 ttttattaaa ttattaaata gatatatttt tatgtaataa attttatgta tttggataaa
    14281 ataatacaaa tgaatatgtt acataaggag tgatgtaaaa tgagtagtgt aaattttgag
    14341 gatttaaaaa acaaatttat taatagtgac ttagatgaaa aaataagaat atatacaaca
    14401 acagaaggtt tatcagtaga acaatttaaa gagctactaa aatattatcc aatacaacat
    14461 ttatctaaac tagaaaaagc acttggatag cagtatttaa tccaaaatta aaaaggcaat
    14521 ttatttaata ttaaaaatat tttttattaa ataaattgcc tttttataat aggtttaaat
    14581 tattatactt ttgatatata attgtagatt ctctcatttc caagttcaat agacccagta
    14641 ttaataagag taactattcc ttctatatct ctagcagtta agtatttata tggaattaat
    14701 aaattataac cagtattact tagtaagttt tttaattggg ttatttgaga gatattgaaa
    14761 cattttgcag ttttagtaga tttttttctt aagttagata aattttcatt ttgaatattt
    14821 attaaagttt ctatatagtt tctataagat atcttaaagt ttgtctcatt gactattttt
    14881 tcattaagta tactactgat aaaatcttga gacaatatat tatctgcaaa tttatctgct
    14941 gttaattttc cagatgttat gcctttttga tattttctaa gaattttttt agtatagcag
    15001 tatattttat ttctcgtttc taaatcgagt tgttctattt gcacaaaacc acttccttct
    15061 aaattataaa aatatttatt aaaaatctat acttaactaa tcaagcttca ataacttatt
    15121 tacaataaat actaaataat aaaatatttg ttatttttta ttattaccaa gtataaaaaa
    15181 tattataatt gtaaaactta caaatatatg taaaatattt aggagtgatg aaaatgttaa
    15241 atgggataat aaagcttttt tattctgaaa ttgtaaaaac aaagattaat aacttagatt
    15301 caagtggata ttctttaaga gatgaatgta aagaaatcag aaatataact ctaaagatag
    15361 atagtgtatt ttttaagctt ttaaagttag cttttggttc aatattgctt atggtagctt
    15421 ttaatttaaa catggtttta ggtataggag tgtttttagt tggtgtagtt tatatactat
    15481 ataaaagaaa tatggaagaa caagtgaaag agcatataaa taatgtgaaa aataatatcg
    15541 acatctctaa aataactttt atggatgaaa aagggagaac aggtctaaat gcattaatta
    15601 cattacttct tataggatta ttgacaggat ttaactggat attagttgct agttttgtcg
    15661 ttgtttttgt ttttactata aagaatgtat gttaaaatac taataaatag tttaatatat
    15721 agggggagaa attgagtatg actaatggca gttataaaat gaagaattta gtggaaatat
    15781 ccgctatggt gactgaatct acagattttt ttaatataaa agatgacata atagaaaaaa
    15841 tgttagaagt tgttcaccct acaaaggcat gtgtgaatct attttataaa aatgattcta
    15901 aatatgccta cttagtatgt tcgcaaacct tagaatatgt acctcaaatt tttccaataa
    15961 atagtcttaa aggtgcaaaa attgattttg acacatatcc agagtatata catgaggctg
    16021 taaatgaaaa aaagataata tatgtggaaa atgtatttga agattctaga gcagaaggtg
    16081 aacgagattt agcaaaagct gaaggttata ttgggagaat agtttttcct tttataataa
    16141 ataacgtagt tgttggattt atgacttgtt ttttaaaaga ggatgattat ataacagaac
    16201 aagatataga ttttatatca tctgttgcat cactcttaag tttatcaata gaaataacta
    16261 ataaaaataa taatgtacaa attttaattg ataaactaag aggctcaata agtgccataa
    16321 atgaagctac taaaaaacta taccttaata agagtattga tggctttttg gaaaatttaa
    16381 gtaaacaagc gtgtaatatt actaatagta aagaagcttt aataattata aatagtcatc
    16441 ataataagga tgctatagtt agttgttata atgttgagca aaaaaagaaa acaaatttat
    16501 atcctatgat tgatatgttt ttaaagaaag agtcattggg ggggtatcta aataatttaa
    16561 aaccatgctc aaaaaaggga ataaatttgg aaagttatat atattacaaa cttcaggata
    16621 aaaatgatac tataggatgt atagtttgtg caaatagcaa aaactatact agtgatgatt
    16681 taaatattct tagtatattg tccaaacaag tttctgttgg tatgcaatta tatgaacata
    16741 atgaaaagga agtaaaacac aaagttcttg aaaacgagtt gaacatatta aataaacaac
    16801 aacagcttat tatggataaa ggtaaaatgg attgtaatga tgagaaagaa ctttattttt
    16861 accataaacc agcaagggta gtaggtggag atttttatta tgctacaaaa gtggatgacg
    16921 aacatatagt ttatattgtt gctgatgtaa tgggacatgg aatggtatca aattatgttg
    16981 ttgcaatgat aaaaggtgct tttaaagtat tatgcaatca atacaataca ccaagagaaa
    17041 taatgacaaa tttaaatagg atgttatatg atgaatttga caagatggga gtatttacaa
    17101 cttgtttgat aggtattata gatactaaag aaaatatgat tactgtgtct aatgcaggtc
    17161 actatagtcc tgtagttgta aagaaagatg gcacaataga aaataattta aattgtaaaa
    17221 agggcatacc tattggaata atggaagatg ctacttatga aaataatact ctttcaatgg
    17281 aagattatgc aatggtatgt atgtacactg atggaatttt agagataaaa aattcatcaa
    17341 aagaagagta tggtataaac cgattagaaa actttctaaa agaaaatttt agattaaatc
    17401 aacagttaat agtagaaaat ctaaaattgg atttaaagaa cttctcatca aaagataatt
    17461 atgatgatga tattttaata gttatgttaa aggataggta gattgattat ggattcaaac
    17521 gataaaaaag gacttaaaag tccagagatt ataataataa atggaagtcc aagaattaat
    17581 aaaaattgca gttttatagc aaatgaagta aaagctatgc tagaagagtg tggattaagt
    17641 tgtaaaatat ttaatatcta caatatggat attgaatatt gcacagcatg tggattttgt
    17701 gaaaaaacag ggtattgcaa gtttaatgat gatatgaagc cattatataa aatgtttgat
    17761 gattcaaaag gaactatagt aataagtcct gtacattttg attcaatatc agctaaatta
    17821 aagactgttg ttgatagaac tcaagctata tatgcaagta aatatgtatt aaataaacct
    17881 tcaattgata gaagtaagaa acggatagga atgtatattg cagtgggggg gagtaaatca
    17941 tatgattcac agtttaaagg tggacagatt gtgatggatt ttttctttaa gagtataaat
    18001 actaaacttt tacacaatat atatttaaac aatacagatg atgtatctta tatagataat
    18061 caagaattta aagatataat atataaaaat ttaaagatat atattgattc aatataattt
    18121 gattttgtag gtaatattat gtataattgt attgcaataa tttaaataat taaaaggagg
    18181 gaatttcaat gaaattacca atagcattat ctaataaaca tgttcatttg agtcaagctg
    18241 atatagacgt attatttggg gcaggtcatg agttaactaa atttaaacca ttatctcaac
    18301 cagggcaata tgcctgtgaa gaaaaagtgg atttagtagg accaaaagga actttaaaag
    18361 gagttagagt actaggacca gctagagctc atactcaagt tgaaatatct ttagcagatg
    18421 gtttcccatt aggagtaaaa gcaccaataa aagaatctgg tgatatagct ggaactccag
    18481 gtgttaaatt agtaggacct gctggagaag ttgaaatgaa agaaggagtt atagttgctt
    18541 ctagacatat acatatgagc aatgaagatg ctgctaaatt tggtgtaact gataagcaaa
    18601 tagtaaaagt taaaacatct ggaccaagag cattagtatt tgaaaatgta ttagtaagag
    18661 ctagtgacag ctttaatcta gaaatgcatg ttgatatgga agagggaaat gcagcaggcg
    18721 ttaaaaatgg agatttagta gaattaatag ttgattaata ttataaatag actttatgct
    18781 agtagatatt ttatttgcta gcatattttt aaatgtaata aatttctata ggataaattt
    18841 aatcgattag attaatttat aaaataaagg ggtattataa aaaatgtaat aaaaacttac
    18901 aaaaatatga tgtaatatgg taaaattata tatata
//
LOCUS       DAMJZE010000070        18894 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJZE010000070 DAMJZE010000000
VERSION     DAMJZE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.070"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(208..1785)
                     /locus_tag="PC541_002058"
     CDS             complement(208..1785)
                     /locus_tag="PC541_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2839524.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="PC541_002059"
     CDS             complement(2082..4493)
                     /locus_tag="PC541_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2839525.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4683..5084
                     /locus_tag="PC541_002060"
     CDS             4683..5084
                     /locus_tag="PC541_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2839526.1"
                     /translation="MILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFLLSNSL
                     SWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLMIDLFS
                     LNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(5149..7266)
                     /locus_tag="PC541_002061"
     CDS             complement(5149..7266)
                     /locus_tag="PC541_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2839527.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(7297..8004)
                     /locus_tag="PC541_002062"
     CDS             complement(7297..8004)
                     /locus_tag="PC541_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2839528.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(8111..9982)
                     /locus_tag="PC541_002063"
     CDS             complement(8111..9982)
                     /locus_tag="PC541_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF2839529.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(10326..12671)
                     /gene="secA"
                     /locus_tag="PC541_002064"
     CDS             complement(10326..12671)
                     /gene="secA"
                     /locus_tag="PC541_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2839530.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
     gene            complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="PC541_002065"
     CDS             complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="PC541_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2839531.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            complement(15380..16978)
                     /locus_tag="PC541_002066"
     CDS             complement(15380..16978)
                     /locus_tag="PC541_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2839532.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            complement(17197..18798)
                     /locus_tag="PC541_002067"
     CDS             complement(17197..18798)
                     /locus_tag="PC541_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2839533.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         5560 a         3554 c         2015 g         7765 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 aaacacttca cttaaatata tatattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatggtt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tgtccctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctatg accttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccgtcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc cgttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcgct atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tcttttaaaa gagttttgtc ttcataagaa atccagaaaa agcctttttc tgagttaaat
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggtctaac acttggtttg
     3661 aacttatctt ttgaataatt atcatcccat cctacaattg aaacagaatg atttaaaggt
     3721 tcacttttat cattacaatt atacgaattt tcatcatcac ttaagtaggc tgaatagtgt
     3781 gcatatcctg atgtcactgc accatattgc attattgcat ttttaacaga agttttatct
     3841 ttactaattc ttacaacatc tgttacatta aattgtgtag gtgcagtatc catattttga
     3901 ggcctagttg ctccttcatc ttctgattta aaattatatg gaatgtcttt ttccagctta
     3961 ggtcctgccc aggctgtaag atatccaatt gcagttacat tcgaactacc tgtcatatca
     4021 gttaagttcc atccatgtat tcctccagtt gaccaccatc taaaatgctc ttctgaaaga
     4081 tcataagttc cataaccttt taatttcaaa tatgcttcta gtgttgacat tccagaaaaa
     4141 gcccaacatg tattaagttt tccttggtct tttgctggtg tagttaaatc taattttctt
     4201 ggatcatatg atacacttga tttatatgat gctggaagac tacttcttac attagtttta
     4261 agtgttgttc cttccatttc ataaggagtt ggtattactc cattatattt tgctatatct
     4321 ccatttttta catcttccaa gtattgaaga tatgattcag aatactctgc agtttctact
     4381 ccagtcagaa ttttattttt ttctgctgag acaggtattg ttgacacaat taaaaaacaa
     4441 actgtcaaca acaccagtag ctttgacaat ttttttaatt tatactttct catatttacc
     4501 cccttgtatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 ttataaaaat aggaattata attatacata tataaaatac tattttatac tgcatattta
     4621 ttgattaata tgtggtatac taattacata ataatatata ccaaataatg gaggaaaatt
     4681 ttttgatatt aaaaaaacat ctagaaacaa tattatatct tttttttggt gctttaacag
     4741 ttttaataaa cattattact tacctatttt ttacaagaat tattaatttt acttttttat
     4801 tatcaaactc tctatcttgg tctatagcag ttttattttc atatataaca aacaaatttt
     4861 ttgtattcaa aagtgaaaaa actgagtttt tatttctatt aaaagaattt gtatcttttt
     4921 tatcttgtcg tttattttct ggttttatag aaatattact tatgtattta atgattgatt
     4981 tattttcttt aaatgatatc ttagtaaaaa taggcactaa tataattgtg ataattttaa
     5041 acttcgtctt cagcaaggta gtagttttta agcatagaaa ttaacattat tatatttatc
     5101 tttatgcaga ataaggagtt gcctaaaaaa aacaactcct tattagcttt aagaaacaat
     5161 ttctatttta tctgttgctc cccatttttc aactttatta ttttctcctg atgctaattc
     5221 tacataaagt ccatttacct tatttccttc taaaattata tcactattag aagtactttt
     5281 ccaactggag tctatttcat ctcctacatt catagcttct ggttttgtag tctttattct
     5341 atagtaaact ttattaccat tgtatccagt tacactaata gttactttag aattttcatc
     5401 aacagaaaca gttgatttta ttccatttgc tactatttta aaaccatcat ctgtcgcacc
     5461 agctcttccc catcttgaaa ctttctttct tgtataatca atttcaacaa cttctatata
     5521 tgatccattc ttagcattta cttttaatgg ttccgtatct tcaaccaagt cccaatcatt
     5581 tatgttaatt ctatcaccaa ccctcatagc tgatggttta tatgacaaaa ctctataata
     5641 aattttataa tttgaatttt taggtttctg aggtacagtt aacataattt gattattctc
     5701 aagaggagaa catgcactta tctttggaga taatcctgct gctgtatcac tatcatcatc
     5761 tttatctgta ttattagtgt taggtttatt gccacttatt cctggttctt tatttccact
     5821 aacagtatca atctttcccg aatttcttat attagtactt gaagaatcat tatctatctt
     5881 tgttatagtt ccatcatttc ttatatttac atcttttgca gaactaaata ttgttacaat
     5941 ccatatttgt ccattatttt cattttcaat cttgcaacca ttatcatcag aaatgtccat
     6001 ttgatttata tatcctgaat tagttaaagt tccatctttt atatctttaa gtatcaatgt
     6061 tttacttata tcacctgtat tattcaactc tccatttggc atatttatag ttattgactg
     6121 actatattct ccatataaat caacctttat agctttttca gtctctaatt taaaagattc
     6181 acttattttt ttatcaggtc taaactttat aatatcatta gcatccactt tctttaaagc
     6241 agaatccaat tcttcaattg tttcaactgt atatttagtt tcttcttgca tatccaatat
     6301 atctttatag acattttcat ttcctattcc accaacctga gtaactgtac taaatatctt
     6361 agtatttagt acatctttct gaatagaatc aattttattt cctactaata ccactggaga
     6421 attgtttttt gcagccaaca ctccaactga tagtgaatct attaaatcac tttgtttttt
     6481 cattccatcc ttggttatat atgcatttct taaatctgtt tctttataaa attcttttat
     6541 aacattagca tttgtttcat ttctattatt tcctgatatc ctcttagaat ttggtaaatt
     6601 tttttctatt tcattagaaa tggaatttaa tcctccaata atataagaat tttttatatt
     6661 ttgggctttt ataaatttat tagctacttc aactccaagg tttgaatctg aaagtataat
     6721 tggcatgtaa ttttgagctg ctattgaacc aacacttaca gcatctgcta atcccttttc
     6781 tccatttact acaattatat cagaaatatc tttttcatta ttcaattttt cagccaattt
     6841 taaggatgtt tcatatctat cattcccaaa aattctttca actgttattt tctctgattt
     6901 taattcattt tctacattta cacctagtgc atttgaacct cctactaaaa atacattttt
     6961 tactccaagc ctttttagtt ctgattttgt cctaaaatcc aattctttac ttccagatag
     7021 taaaataggt gcatctttca atttagcaaa tggagttgca gataatgcat ctgctattga
     7081 gttatcattc actaatacag cattatcaga tcttaaccaa ccgttttggc ttatttttat
     7141 tgctgtttcc catctacctg aacctattaa actgtcttta ttagatgcat ttaccacatt
     7201 actaggtaaa acaattgata aaaatgctgc tgttaataaa gatattattt tttttgatat
     7261 ttgcattaat tatctcctcc taatttaaat ataattttat ggagcatgta catactgtgt
     7321 tttatttaat tctaaatcat caaaatgaga ttttactgaa tggtatccta tagcataatg
     7381 tccactttca ggattccatt tcttatatgc attcatcgca cttaataact tctcctcttg
     7441 actaggtaat accttccaga cttctacatt tggattaact tttttaaaat ccttatagtc
     7501 atatggttct acataaaatc ttaaatcttt tatttttcct tctctatata actctagtgc
     7561 agcttctcct aatgctctat gatcttcatg tattccacta gctttaaacg gagtaacagt
     7621 ttttactttt gcttctggat attcttctaa gtatttaagt attatttcct ttgccttttc
     7681 tttgcttaac tcaccatcat gagctctatt atcttctata tgtatattac tttcttttac
     7741 acccattgct tcacagctat ccttaaattc ttcatctcta tcttttgaaa attcactttc
     7801 tgataaagag tatttatgaa catctttaat atgataagaa catgaccctc catcatccaa
     7861 aacttttctt attatagatt tactaccatc tgtgcacaat atgacatgtg tatcactttt
     7921 ttcaacatgt tcaattatag cacttcccat acttaaggtt tcatcatctt gatgtggtgt
     7981 aaaaaacaaa gttgcatcta ccactactac atcctcctac tttataaaat gttataattt
     8041 acaataaata gcataattat agaaaaagat tcctgtatta attaaggaat cttctccaca
     8101 gtataagtta ttaccaacct agtattttag ctatcttttg tattactgtc aattcaactc
     8161 cattacctat ttgagttact tctgcaccaa gatttaattt tttactaagt gcattctttt
     8221 gtgagtctgt tatatcagtt ttagctagaa gtattggacc tttacctgct gctagaggtc
     8281 ctgctgccaa tgcatctact agcataccat tatttccata tccatctttt gctatatata
     8341 gtttatctaa tttgccataa tatgtttcta taactttagc attagtatcg tgtcttgttt
     8401 ctccttcaac tctttgaact tttctatctt tatcaatatc tgcaagttga ttttcaattt
     8461 ggctagatac attattagag ccaccaacta tgccaatctc ttttccatcc atcaatttga
     8521 tagcatctgc tgatagatca ttccaaccat ttactattat aggagcctta agttcagcag
     8581 ctttagcagc tatactcata gcatcagcct ccccttttgc gcctactaca aatgcatctt
     8641 taaaagaccc catagctttt gctactgcta cagaagtcat atgtctatca tctccagcta
     8701 gtctttcaac attcttagtt actgattcta attgcttttt agctgtattt gatactgctg
     8761 attctccacc tactatatat attttagctg atgggctttt cttaattata tcttttatat
     8821 aatctaaagt tacttttggt atttcattat cagatgctaa taatataggt gcttttttag
     8881 attgagcaag tggtgtagct gctaatccgt caactagttt atttgaatta actataacta
     8941 tattgtcagt atattttgtt tgttttgcta tagccactgc agttgcatat ctatcatctc
     9001 cagctatcaa tttaaccttt gaattacctt ctaatgcagc tttggcatct tttaaatctt
     9061 tttcattggt actttctaca gtaagctctg ttgcttttcc agatgcatca gtatacttaa
     9121 tagtaaattt atattttcct gtttctgaat cttttgctga aatatcacta attgactttg
     9181 atgtaccatt tgctaccata ttacctttaa cagtcaatct atatccatca tataaatctt
     9241 gtggtgaaac tgtatttagg tcactatttt ttatagttat ttcggccaat tggacatcat
     9301 caatattttt agctcctgct acacctagtt tatcgatttt atttactcta aatccttctg
     9361 cagaagggtc taaatttgtt ccagttgaat caactggatt tgctgttaaa tcaacttttt
     9421 catcaccaat acctacatat atagttttag aagttactgt ttctgtttta tctcttaaag
     9481 ttataactaa cttatcttta gcactatcat atgaagcttt tacatctgca actttataaa
     9541 ttccattggt ttcagtttta gctttttctg ttatagaatt agcctcagcg gaaaggtctg
     9601 ctgaattttc atatttttca gttgcttctg caactacttc atttgcacta tttgttatat
     9661 gtcctttatc tgttatagtt acaataagtt ctttattttc tcccaaagca tttactaatt
     9721 tctctaattc atctatatta gttattattt ttaattttga taaagtctca ccaactttta
     9781 tttcatatat tggttgtcca acttgagaac ctcctgtata cttttgagac attaaacctc
     9841 taactttcga aactaattct gtagcctctt tcttagttat tgtttctttt tttacctgtg
     9901 tagtttctgc agcaaaaact ggagctactg atgtacttat cattgcagca gccataatta
     9961 cagaaagatt ttttttattc atggtatttt ccccctaaaa aattcaaatt tttattattt
    10021 tttccttatt taccaattat aatatatatt tgatattatt tcaataaatg agacttattg
    10081 taataattaa ccaaaagata taaacttttt cttatatttt atttttttaa tatttacgta
    10141 tacaaaaaag taacaaaatt gtaatttttt ttaataatcc agtttataac taatagactt
    10201 ttcatcttct ttatagtata atattaaatg gaagaaagaa atttcaagca tctctttttt
    10261 ccgtatttct cacatagaaa aagaggctga ttatcagcct ctttttccta tacagtttaa
    10321 atttcttaat taaatttata taaatactgt atagtcgtct ctcttatatt tttatttaat
    10381 gattcaaaca tctcataacc ttccatagca tattctttga aaggatcttt ttgagcatat
    10441 gatttaaatc ctacatattg ttttaattgc tccatagcat ctatatgttc tacccaatat
    10501 tgatcaacta cttttaaaag aacaattttt tccatttcgg ttattttgtc aaggcctact
    10561 accatctttt ttaaatcata aactcttttt gatatttcat aagtactatc aattatatcc
    10621 tcaatacttt tattatctaa gtcaggtatt agtaaggtat cagctggcat aaatgtatta
    10681 taaagatgct taaaatatcc atagtaatcc ctctttcttt ttgttaaata ttcagcagca
    10741 gcttctttta ttatatcttt aaccatgttt tgaatatcta cttgtatatc ttcatcattt
    10801 agaactttat ttctttcgtt atagattact tttctttgtt catttatagt atcatcatat
    10861 ttaagaacat ttttccttaa ttcaaaattt ttaccttcca cccctttttg agctctctct
    10921 attgcctttg taagagattt actttcaata gaattatttt tatttgaatt tggtcgtttc
    10981 attagtttct ctatactttt tccaccatac aatttgatta catcatcttc aagactaaca
    11041 aaaaaccttg atgttcctgg atcaccttga cgtccagaac gccctctaag ctgattatcg
    11101 attcttctag attcatgtct ctcagttcct attacataaa gtcctcctaa ttcctttact
    11161 ctactttctt cttctttatc acctgctcct aaagcaatat ctgttcctct acctgccata
    11221 ttagttgcaa tagttactga acccaactta ccagcctttg acactatcaa tgcttcttta
    11281 tcattttcct tagcatttag aacttgatgt tttattcctt ctttcttaag cataccagat
    11341 aacatttctg atttctcaac agaaacagta ccaataagaa ttggctgttt tgttttatgt
    11401 atccttatta cttcttctac aacagcttga tacttttctt tctctgttct aaacacttta
    11461 tcatgaagat ctgctcttat aattggtttg ttagtaggaa tttgtacaac atttaattta
    11521 taaattgatt caaattctcc ttcttcagtt ttagcagtcc ctgtcatacc agaaagttta
    11581 ttatataatc taaagaaatt ctggaatgta attgtagcca tagttttaga ttcatctttt
    11641 atttctaccc cttcttttgc ttctatagct tggtgtaagc catctgtata tcttcttcca
    11701 tccattactc ttcctgtaaa ttcatcaact atcattactt cttgattaac aacaacataa
    11761 tcaacatctt tttccataag tttgtgaccc ctaagagcct gatttatatg atgatataat
    11821 tctatatttt ctatatctgt aagattttta atcccaaaaa atgattctgc cttatcaata
    11881 ccactttcct ttagagctac ggtcttatct tttctatcta attcataatc ttcttctttt
    11941 attgttttta taaatgaatt tgctaattca taaagttttg aatcttcatc accctctcca
    12001 gcaattataa gtggagttct agcttcatct ataagaattg agtcaacctc atcaactatg
    12061 gcaaaattca actctctttg tactttatca aactttttag ttaccatatt atcttttaaa
    12121 taatcaaatc caaattcatt atttgtacca taagtaatat cacaattgta ttgaactttt
    12181 ctaacttcag gtataagatc tgcaataatt actcctacag acatacctaa aaactcatac
    12241 actggttgca ttagctcttt atcacgttgt gctagatagt catttactgt aacaacatgc
    12301 acacctttac ctgttaaagc atttagataa acaggtgcaa cttctaccaa agttttacct
    12361 tcaccagttt tcatttctgc tatttttcct tgatgaatta caatgcctcc aattaattga
    12421 accctatatt gacgcattcc taaaactctt tttgaagctt ctcttgccac tgcaaatgct
    12481 tctggtaata aactatctaa agtttctcct ttttttaatc tatctctaaa taggtctgtc
    12541 atttttttta attcgtcatc agacatattt tctatatctt tttctaaaga ttctatttta
    12601 tcaactatca cattaagcct tcttatttcc tgctcatctg ctttatccaa tattgtatcc
    12661 aaaactgaca tatttaattt acctcctttt ttatcataca attactatta taccttaaaa
    12721 ttatagaata gtagacaatt atttttattt atagtataat aatcatactt aaataaatta
    12781 catctttaat tatttaggta tttctcacaa aaaaaggaac tatttttagt tcctttttta
    12841 atttcttttt ataaaaaaga gaacttcttc aaagaagttc cctcttattt atagtttaat
    12901 tttatcttat aattctaata aatcttttat tctcttaata actgaatctg ctattccttt
    12961 accaacttga gtcaatttct tagaatcatc agaatttgta actttactta ttgctactga
    13021 ttggtcagaa gataaactat cagttgctaa aactattgga gcttcattgt gacctgctac
    13081 tgctgctatt gctaacgcat ctactaattg atcttctttt gtagaaccat ctttagcaac
    13141 atagaagttt ttaacacctg tgctaattac actgtcacta ttatcttttt caaaataagt
    13201 ttttataact tctgcattag tatcttgtct atcatctcca ctaactcttt ctggactctt
    13261 acctgtagca tcatctatag catcttccat gtcttcagaa acactagatt ttccacctat
    13321 tatatctaca taagcacttc ctataaagtc ttttgcatca gttgataaat cttttccttt
    13381 tccatcaact actactattg gcatttcttt attagaagca actgatgcta tactcattgc
    13441 atctgctaat ccagtaccac caactacaaa tactttatta tggtttaaac ctatttcatc
    13501 agcaatttct aaagatgtag catatctatc atcaccagat aatctttcaa cttttaatcc
    13561 catatctttt aattcatttg ctacttcttt agatacagaa ttttccccac cagctatata
    13621 aacagtcttt ttagatgtta taccagtttt atcatctaat cccattactc tctttatttc
    13681 agattttact gatgaatcta acttatcttt tgaagttaat aataatggag catctttaac
    13741 agcagctaaa ggtgaagcta ctagaccatc tactattgat tgtgatccta ctaatactac
    13801 ctcattagct tctacataat ctcctttaat tcctccatct gcattataag agtctttact
    13861 tatttctatt gcagtctcta ttctatcatc accagctaat acagaattat ttgaataaga
    13921 attattataa gttcttaatt catctaaagc tgattttaag ttattcttat tagtagatct
    13981 taatgttatt atagcaggag tatctactct atcacttgta ggtaattctg gcttatagtt
    14041 agatgatgca tatgttgaag ctgtatttaa tcttttacct gttggataaa gaactacctc
    14101 ataataatca ttgtcagttt tttcaaaatc acttgctgtt actgtcttat atgcatcttc
    14161 taattcagta tctttaaata catatttttt agctaaagct tctgcttttt cttttgcatc
    14221 tccagatatt gtaactgttt catctgatgc tgctataact gttacatttg attcaactgc
    14281 tccaactttt tctccagttt tttcaaaact taattttgat gtaccatcag tagtattaac
    14341 cgttataact ggtttactaa aatcatatat ctctttagta ccatcaagag atgctactgc
    14401 tgtatctaaa gtaggtgcta ctgctgcttg tgtatcatat tttaatttaa ttgcaccagc
    14461 tactgcatca gcatatgttg ctgtaatagc tactgttcct gtagcattat taccagtctt
    14521 atcagtaaat tttgaagttt tgactgctgc ctttgtagct gctaaaactt catctttagt
    14581 agctccatct acataagctg acgtagttgt atcatatttt tgtaacttta atttagcatc
    14641 attttctatt gaatttccat cttgatctac aatcttaact gttgttgcag taccaacttt
    14701 agtaactaca gcattactat caagttcctt taataatgta tcatcatatt ttttttctgt
    14761 tccatctgta atttttacag atatttttgc atattctcca gcttttaaat ctttaatttc
    14821 atctttaaca taacttattt gacttgcttc taaacttgat atagataccc cttttggatt
    14881 tccttttttg tcaaaaaagt taactgttat gcctgttaca ccatctgcaa catatttttt
    14941 aagtattctt tctatatcac tatacttact actagataca gtggtatttc catttggaaa
    15001 actatcctct gctgcagcga aaactggtgc tgctgatccg attactgtaa cggcagacat
    15061 tacaactgcc aaatttttct tattcattcc ttaaattcct cccaacatta tattaattac
    15121 atctctttaa ttatgtacat taaaattcca tgttcatata aaccaaatat ttctcacact
    15181 cttatgatag actatataaa atagaatttc aataatttat gcaaaattaa tagaattttc
    15241 tttcaacatt tttccccttt tttttgctat ttcatatttt ttaaattaaa ttttcaattt
    15301 taattttatt aatacattta tttatctgat ttaatcaata tatattaata ttttctacca
    15361 taaaatatta atatatactc taactattta aaaattccat tgtagcatta acttctcttt
    15421 gattagaaac atacattcca aagccaaaac tataaaactc ttcctttaac atattatcat
    15481 tatacttaga agacttcttc cataaatcaa atacagactt agcaatctct tttgcatctt
    15541 tagatgtata tacagactta acattcatag gtaaatatgc aatattttca tcagttctca
    15601 ttccaaagcc agaaaatact tcagctgcat ttttcccatc tatttcatga gcaaatatct
    15661 tcttctcatc catatactta gaccatgcat ttgccattcc agctaaagaa tctaattctc
    15721 ttaaactttt cttacctttt tcttttctat atgaattaac tagattatac atttcattag
    15781 ataccatagc cttaaactca taactattag ggtcttttgt acttgtgttt ataattactt
    15841 tgacatttac attaatctca cacttttgcc caaaattatc aaaagcactt acatttaaaa
    15901 catacgttcc tgccttactg gtatccacag ttccctcaat attaggcttc aaattatttc
    15961 cataataatc agtggcaact atattcaaca tactattatc aaacttatcc cctacagaaa
    16021 tctctacatc attagcagtg attgtaggca ttgtattttt atcagatgca gaactaatac
    16081 attggtcagc aaccttctta tccattccac ctaaagaagt aacttcatca gcacctctaa
    16141 gcacactctt atcacttcca tcattaacaa gtactatagg tttgtcctta gccaacggag
    16201 aaccagccac agcatcaact aacttatacc cttgagaaac ataaaacttc tttgttcctt
    16261 tataaaagcg ttgtataacc ttcttattag tttcaaatct atctttacca gcaatcctaa
    16321 cagaattagt cttactcaca agttcattac tcattatctc ctctgaccca agagaataac
    16381 attgaacgcc atcaacctta aatggtatac tctttccatc agtaagtata acaggaactc
    16441 catctctagc agctacagaa gatacactca ttgcatcagc ctcacctgtg tatccattta
    16501 caagaaaaac cttatcacca tcatttactg gtttaatagc tgaaatctcc ttagctataa
    16561 gatagctagt ctttattcta tcttccccac caattctttt aacttcaatc cctttattct
    16621 taagttgatt ttctacagat ttacctattg tatcctctgt acctataaca taagcattct
    16681 taacatcctt tagtcttttt tcaacatctg ttggtatctt atctctttgc actaacatta
    16741 taggagcttt tacaactcca gataatccac ttgcacttaa tccatctact atagaattat
    16801 cagtatttac taatatgact gtatcatata tttgtttatc tgctatcaat gcagaagtct
    16861 catatctatc tttcccttgt atcttttcta tactcgaaag tgcattgata tttggtgctc
    16921 ctgccattat taatgatatt gtcaatccta ttgataatac tctcttattt accttcatcg
    16981 aactcctcct actaattttt ttatataatt cagctaatca taaattacac cctcagtagt
    17041 gattccctca aaatttataa actactaaaa caaaaaacac ctaaaatacc ataacctgta
    17101 aagcaataat aaaacagatt acaaaaagta ctttagatgt gattttcatt ttataatatc
    17161 agtaaattaa caaaaaaaca agatttatgg aattttttac aagctacctt cattgccatt
    17221 taaaaactct tgagtagcat gcacttgacc atcagataat atataaagcc caaatccagt
    17281 actataaaac tcatcactaa gcatgtttgc attgtattct ggagatttct tccacacttc
    17341 aaatatagcc tttgccaagt cttttgcatc ttgagttgtt tgaacactct tagaatctat
    17401 ataaatatat gctatatttt cctcacttct cattccaaac tcagaaaata cttgtggagc
    17461 atttttgcca tctatataat gttcaaaaac tttgttatcc atcatatact tagaccatgc
    17521 atttgccata ccttctagct tactagatac taccaaaggc tctttacctt tttcttttct
    17581 ataggaattg attaagttat acatttcagt agataccatt tttttaaatt ctgggctgtt
    17641 gtaatcatga gatttatcat ccaacacttt tatttctact ctttttcctg tactttttcc
    17701 ccattcgtct gtagctttta atgtcaatat gtatgtacct actctatttg tatctataaa
    17761 cccatcaact tctattggta ataccttccc agtgttatct ttagctacta tatttaactt
    17821 gccagtgtca aacttttcac ccttatatac ctctgtactt ccaactgtaa ttgctggggg
    17881 ttgtccattt gactttgaag cacttataca ttgttgtatt accttctcat ctatttcacc
    17941 tacagaggtt atattcttag ctccttctaa tacagtttta tcgcttccat catttactag
    18001 taccattgga gagtttttag taagtggtgc agctgctatt gcatctacta attggtatcc
    18061 atctgataca tagaatccat ctgcactatt aaaaaagtag tctattacgt ttttgttagt
    18121 ttcaaatcgg tcagttcctt ctattcttac tgagtttgta tttttaacta gaggattact
    18181 cattatctcc tctgaaccta tacaatatga ttgaatattt tttacatcaa aaggcacact
    18241 ctttccatct gtaagtatga ttggagctcc atctctagta gctactgatg atacactcat
    18301 tgcatctgct tctccactat atgcattagt tagtagtact ttatcaactt tttttatagt
    18361 tgctatttct ttagctatta aatagcttgt ttttagtcta tcttctccac caattctttt
    18421 gacttctata tttttagaat ctagttcttt ttctactgat ttacttatag tatcttctgt
    18481 acctattata tatgcttttt tgatattttt tagacatcta tttgtgtctg ctggtatctt
    18541 attttgttgt gtaaatagta ttggagcttt tgtagctccc gataatccac ttgcacttaa
    18601 tccatctgca agtgattttt ctgtatttac taaaattact gtttcataag tctgtttatc
    18661 agctatcttt gctgctgttt catatctatc tttaccgtat attttttcta ctactgatga
    18721 tgcatttaca cttttgaagt ttactaagat taatgatact gctagaccta atgataatat
    18781 ttttttgttc attttcatat ccccctgatg atttttatat tttatattta tattgtgatt
    18841 attttccatt tattttctct ttattttcca tatattatag agtatatatg atat
//
LOCUS       DAMJZE010000071        18635 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJZE010000071 DAMJZE010000000
VERSION     DAMJZE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.071"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..538)
                     /gene="yqeC"
                     /locus_tag="PC541_002068"
     CDS             complement(<1..538)
                     /gene="yqeC"
                     /locus_tag="PC541_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2839534.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKN"
     gene            complement(721..1998)
                     /locus_tag="PC541_002069"
     CDS             complement(721..1998)
                     /locus_tag="PC541_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2839535.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(2137..4443)
                     /locus_tag="PC541_002070"
     CDS             complement(2137..4443)
                     /locus_tag="PC541_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2839536.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(4436..5230)
                     /locus_tag="PC541_002071"
     CDS             complement(4436..5230)
                     /locus_tag="PC541_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2839537.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(5337..6695)
                     /locus_tag="PC541_002072"
     CDS             complement(5337..6695)
                     /locus_tag="PC541_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2839538.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(8042..9091)
                     /locus_tag="PC541_002073"
     CDS             complement(8042..9091)
                     /locus_tag="PC541_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839539.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQ
                     EMIVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEI
                     NVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITV
                     MKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMK
                     EIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
     gene            complement(9321..10406)
                     /locus_tag="PC541_002074"
     CDS             complement(9321..10406)
                     /locus_tag="PC541_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2839540.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(10442..11755)
                     /locus_tag="PC541_002075"
     CDS             complement(10442..11755)
                     /locus_tag="PC541_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2839541.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(11756..14047)
                     /locus_tag="PC541_002076"
     CDS             complement(11756..14047)
                     /locus_tag="PC541_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2839542.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(14037..14831)
                     /locus_tag="PC541_002077"
     CDS             complement(14037..14831)
                     /locus_tag="PC541_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2839543.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            complement(14870..15316)
                     /locus_tag="PC541_002078"
     CDS             complement(14870..15316)
                     /locus_tag="PC541_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2839544.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(15388..16794)
                     /locus_tag="PC541_002079"
     CDS             complement(15388..16794)
                     /locus_tag="PC541_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839545.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            complement(16812..18182)
                     /gene="hydA"
                     /locus_tag="PC541_002080"
     CDS             complement(16812..18182)
                     /gene="hydA"
                     /locus_tag="PC541_002080"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2839546.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(18315..>18635)
                     /locus_tag="PC541_002081"
     CDS             complement(18315..>18635)
                     /locus_tag="PC541_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2839547.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         5584 a         3634 c         1978 g         7439 t
ORIGIN      
        1 tattttttaa gttttcaata cttactgtag aactactatt aatactactt gtatctaaat
       61 ttgctatttt tttaaatatt tctactctat gtatattctc ttcattaata ttcattccaa
      121 atgatgttat atctaatatc cctatggttt ttgtagttct atgatatact actggttctt
      181 tagcattcca acctttaagt ttttttctct ttgagccatc tccctctatt aaaactaaat
      241 caaattggtc tacaatttca tccaaaatag aggaatcaat acttactaat ttattttcat
      301 tatttatata actgccacag actgttattc catgacaaat agatggaata acagttccat
      361 ctattgtaat aattatattg tcataatcgt ttggtacata tattttagtt gttgttgtaa
      421 gaagaacttt caatttattt ctataaaaat ttgcaaaata gtttataaac gaagtttttc
      481 ctcctgctcc aactacagtt attatttcat tcttttctat tatatttgat agttccacac
      541 tttcacctct tataaccagt tttctaatct cttaattatt atagttaggc ctttttagta
      601 aaagtgattt ttgaaattcc ataaactcga aagtaaatag attctgttgt aaacaacaag
      661 gcggatccaa ttcgccgcta agctgtaagc ttacgctatc agtttatctt ttaaatataa
      721 ttaagatatt cttactctct gtggttgctt taatatttga ggattattct gtaaattaga
      781 taaattaact acttgtgatt tttttagtat attaaaactc gcatttacat ctgcatttat
      841 aatgattcca acactggatt tgtactgtcc acgtgttatt ctttcccctt taaagtcata
      901 ttttttaaca ttgtccacat tgatatcagg tagaaaatca tcagcaaaaa aatctgattt
      961 actagtatag ctttcttctt gcctaataaa attgatattt tttattttac acagatattc
     1021 taattttcct tttatctctc caaatgggat tcttataaaa tttctatata tttttctctc
     1081 catattatca cttttttcta acttagcatt atatcctaca attaaatttc caatattatt
     1141 ttttacacaa taatcaatta tataaaaaca ggttttattt acatagtctt taacttgatt
     1201 atttctctta ttccaaactt taaattgttt tcttgttatt gcagttatat tcttttttaa
     1261 ttttaaggct tctattttat cattctgttt gtttgcccac tgattcacag attttagcct
     1321 ttttccatct atgataaaac tatcacctaa attggtaaca catgtacata aattttctaa
     1381 acccaagtct atagctagtg catttttttg atttgaaact tcctctgatt ttggtatttc
     1441 ataacaatac tgaatttcaa agaacctagc tttatgttta ggtattattc ttatttcttt
     1501 aatttttttc tctagtatat tttgtggaac ttttatactt actttcccat atagtttttt
     1561 aaatgctgga gacataggta catttaaaat cccatcttct ttgattctta tttgacctat
     1621 tattatgcta aaaaatcctt ctttatctaa atatttaggt gattttatcc ctctaaagtc
     1681 atattttcct tctttggcta atttaattaa tccaaaaaat gacctaaacg aactgtctac
     1741 ttcttttaat atttgttgcg ctatattact attgagaagc ttatagttct cattttcttt
     1801 gcatacatga taattagctt catagttaag atgttctcct tgattgaaat aatgctgtct
     1861 tacattatat gtaccaacat tatataagtt cttagctaag aagcacatct ctcgtagagc
     1921 ttgatattct tttttagtta agttttttaa ttgttgtctc tgagttccat agtgtatatt
     1981 ttttattctg tttgacatga actcacctcc tttagaatat actctactag acattatata
     2041 cggtattttt acaaaacacc atatataatg tccatttaat tactttattt tacaaaataa
     2101 tttaaattta cttgctttta tgcatcttat tatttattat aattgtttat tattattttt
     2161 catacccata taaacttttt caaaagtcat aggaagctct cttaaacgaa ctcctgtagc
     2221 attaaatatt gcatttgcta tagctggtgc tggtgtatgt ataactattt ctcctaatga
     2281 tttagcacca taaggacctc ctgggtcgaa attctcagca aaatctacca atatatttcc
     2341 aacttctttt tttgtaggaa ctttatactg taggaaacta tctgttaata atctaccatc
     2401 tttatcaaat atagggtctt catacattgc aaaccctata cccatcatca caccaccttc
     2461 tacttgtatt ctagctaaag ttggatttat aactgttcca caatcaggta cacatgcaaa
     2521 atttatgact ttatattcac ctgtattttt atctaattct atctctgcaa atcctgctat
     2581 aaatggtttt atttttttca acatgccaaa agattctgtt gcttccaaaa ctactggtac
     2641 atcatcacta gcaaatccta ttcctccaac agattctcta cccaattcct ctaatagaac
     2701 aaacttgcta ttatcgtttt taacaaaaac cctgtctttt cccaactcta aatcttcttt
     2761 tttaaatcct agctttgact ctgcaactga aagtaatata tcttttaatt tatttgctgt
     2821 ctttattgtt gcatttcctg taaccgttgt cgtacaagaa gcaaatgctc ctgtatcata
     2881 gggacataag tctgaatcac ctgtataaat agaaattcta tctacagtag tatttaaagc
     2941 ttccgcagca atttgagctt gaattgtatc agccccttga cctaagtcag caagaccaga
     3001 taatatttta tatgtgccat cttcatccat tcttatagtg actgttgcca tatctacacg
     3061 cggtatacca gaaccatgat ttgctactgc catacctact gctcttactt tgttgtttcc
     3121 tatatcttta catggatatt tatcatccca attaattaat ttctttccct tttctataca
     3181 ttgtctcagc tcacaactta atatttcgtc ttcagctgct tttccattgg tatacctatc
     3241 tattatattt ttaattctaa attcaagtgg gtccattcca atcttatgtg ccaattcatc
     3301 aatagcacta tctattgcaa atattccctg tgtagcacca taccctctaa gtgctcctgc
     3361 tggtaaccta ttagtatata ctgttctagc attaaatcta acagcatcaa tatcattata
     3421 tattggtaag gtattgtgtc cagattcgga agaaacagag acaccattcc caccataagc
     3481 acctgtattg tttaatgact ttagctctat tgcttttaaa tttccttctt tatctgcacc
     3541 aactttaaca tcaaaaaaca tttcatgtct actattagaa cctacaaaag tttcacttct
     3601 tgtaaataca aattttgctg gtctaccagt tttcatagta acaaatgcag taaatatatc
     3661 tgttgctgct atattctttc ctccaaatcc accacctatt ctaggtttaa ttactcttat
     3721 ttttgaagac ggtataccca atacttttct aagtattctc tttacattgt gtggtgattg
     3781 taatgatgaa actacaacaa ttctatcatt tatatcttta tatgaatatg acctatgagt
     3841 ttccatcatg caatgcgatt gactttgggt tttgtatgaa gattcaacca caaaatcact
     3901 ttctttaagc ttagcttcaa catctccaaa ttcaaaaaat tcttttgata ctatattatt
     3961 ttttgcatcc aatccaaaag ggtatggctc tactatgtct ttttctggat gtataacaac
     4021 ttcatttcct tctgcttctc ttgcatcaaa cacaggcttt aaaacctcat aatctacctt
     4081 tatcattttt aaggctttaa gtgctgtttt ctcatcttca gcagccacta aagctacttc
     4141 atctccaata aatctaactg tatcttcaag taaaactcta tcataaggtg atgcttctgg
     4201 ttctgtttga cctggaacac aatatctgac ctgtggtaca tctttatacg tataaatacc
     4261 cacaacccct ggaatttttt cggcttttga tgtatctata ttctttatct tagcatgagc
     4321 atgtggactt ctaagtagtt ttaatgtcaa aacatcttta tgcataatga ggtcttcagt
     4381 atatagaggt tttcctgtga caattgctgt actatctatc tttcttatcc ccttattaat
     4441 cattatacat tccctcctca atagcattat acatcctttt aactaatgca cttgccatat
     4501 cctttctgta ctctttacta gctctcatat tacttccata agtaagttcc ttactagcaa
     4561 caatagatgc cttatctata tctttttctt tatttaatat attactcgct tcaagtgcta
     4621 atctagcttt ttgaggtctt gccccaattg ctattttgta agtatcattt tcttttagca
     4681 ttgctgaatt taatacagaa aaatctcctg tgcattttct tattgagtca aaaacagcta
     4741 ttgcatcttt ttttggtagt attacttcga ccaatatatc cctacttaat tcactatcca
     4801 aaaactcact taactctagt attcctttat tgtataatct caccttcgct ccaacaacca
     4861 aaagtgctgg tattaagtca gaaaagccat actttgaaaa tacacttgca ccaattctag
     4921 ccccttgtct aaattgaaca ccaactatat tagaaacagc gcttgaaagt attccactac
     4981 agtaattttt tatagtttta tcaatttcta aacttctaag tgaagtatct gcacctatca
     5041 aaatattttt tttatcctct tttatataat ttagatttat atcttttaaa tctatcgctg
     5101 ttcctatatt tttattactt aattttataa atccacatcc acctaaaata acattatttt
     5161 ttttagaaat taaaatttta tatgcttctt ctaaactctg tggaactgaa tattccttta
     5221 tagtaaccat cttatccccc catcattaac ttattaaaac acaaaagttt ttaataaatc
     5281 ttttatttat atttataatt gtagtatatt taattcacat atattaaacc tttacattat
     5341 aaacttttat ttttatcctc aaccttatca cttttatttc caggaaattt taccatatat
     5401 aaagcttgaa ttattatggc taccattgct cctgaaacta cacttgaatc tgcaagtaat
     5461 gtctctataa attttggaaa tttttcaaga acttgaggag ctaccatgaa tccaatacca
     5521 gaagccatag aaagacctaa tatcatgcta ttttcttgat taaatccatc catactaaca
     5581 agtcttaaac cagatgttgt aagagttgaa aatataacga gtgttgctcc tccaaccaca
     5641 cagggaggaa ttgttcttat taaattagct aataaaggcg atattcctgc caaaaataat
     5701 ccaattcctc caatagctag tacaaattta cttacaacct ttgtattgga aacgattgca
     5761 gaattttgtc caaacatccc cattggagtt gcagaaaata atgcacctat tgtagagagt
     5821 actccatttc ctaaaacagc agaagaaagt tcttcatctg ttacttgtct attaagccct
     5881 cctaccgcag aaagtgtaca agcacccatt atatcagcta tctcaactaa atatattatt
     5941 gaaaacatta caacaacttc aagtcttata tctagcccaa aagcaagtgg ttttggaagt
     6001 gcaaccaaag taaactcctg aattgaagaa aaatttacta tacccaaaat taatgataaa
     6061 atgtatccaa aaattatgct aactaatatt gatgcatctt taaataatcc ttttccatac
     6121 ttatttaatc ctaatatcat aagagataca gatattccaa ctataaaatt aattggattc
     6181 ccaaaagtct cattgcccat acccccagct aagtttttta tagctattgg aaaaagtcca
     6241 agacccatac atgcaactat tgttcctgat acagctggag taaatataaa tcgtattttt
     6301 ttaagaccaa aaccaattat aattattact ataccagcta ttatttgaga gccaaataat
     6361 actggtagtc catacttact tgcaacagat accccagctc ctataaacat agctgtagct
     6421 cccattataa ctggtattcc tcctcctaat ttaaaacctt taaacaaagg tattggatat
     6481 aattgtaata aagttgataa tcctgcaaaa actaatgcgg cttgaatcaa taatgttgac
     6541 atcttatgat taagtccagc tgcatttgca atcaatatcg ctggtaaaca actacctatt
     6601 accattgcca ttaagtgttg taaccctaaa ggtattgctt cctttagtgg tggtattccg
     6661 tctaattcaa attttgatgt actttttgct ttcataataa aaactcccgt tacataaaac
     6721 ttattgaatt atataaatac ttaaaaatct aatatattaa attaatgatg ttcataaata
     6781 tcaactatac tccaacatat atttaagttt ttagtcttaa aaaacagatg caataaattt
     6841 aattattaac aaatacacgt attctttaat tttgtataca ttatatacca tgctttacaa
     6901 atataagagt attatagcta tgaattttta attttttata gtaaatttaa ttcaatttaa
     6961 atctttcaat cttaatcatt ataagttata agttttcata agtatttaca tgtttcaagt
     7021 tttaaacatt tacctataaa cgctacttta tagatgtcac ttcaaatcca gtgacctcat
     7081 actttcatat gagcgtagac tatttcttca tcttcagcat tacctgctaa gagcaacccg
     7141 tttccactat caatcgctta tagtgtactc tctctcgaga tagtcgtttg accttcctat
     7201 ttctaggctt ggcgaccaaa tgtccatttt taagtactta ggctctcacc atataccatc
     7261 ctcactattg tttctggttt tcaccacatt cataaaagtt tctttctatc ttttattgta
     7321 gttgcaaggc tttaggattt actggtttta aagttgtgtc ctacatagat ttctctatgt
     7381 acggggccaa aagtacaatt tatttaacat actttgcttt accccctgta aataatatta
     7441 tttactcttt cactgttttt gttattaaat taattaaata tcaattaatc taagcataaa
     7501 ataattattt aatttaaact caaaatattt atgaatattt tataatataa ttctcataag
     7561 acagaaaaac ttaataagct atgtatctca gttgtaccaa tgtactctcc tttactatat
     7621 ttgtatctgc aacataaaat atacaagttt ctatctattt atagatatat atatctataa
     7681 gcaaaatagt aatattattt ttatagtaaa aattttattc atattactat ttataatttt
     7741 tatagagcaa ttttagtgcc aatatttgca tacatttgta tagtataaaa tttttatgtt
     7801 ctcattgata aaattttact tatttcaagc gttctattag attttatcat ctaaatttta
     7861 ggttttattt ttataaatta attgtattta cataaaaaaa aagacctaaa aacaaatttt
     7921 taacggtctt ttctcaattt tttattttca tttctaaaat ttataataaa tagtaagaac
     7981 actataaatt cttttatata aaattcttag tttgtggatg ataaattagt gaattaaaat
     8041 actactttac tgattttagt ttgacaatat cactccaatt tttactagtg gctaactcta
     8101 ctaaatttaa gtctcttctt atttcagcta tatcctcttt tatagaatta gtgtcacctt
     8161 ttatatatgc tatgttatgt tctatattat catgagtagc tttatttatt tcagaattat
     8221 gtaataaact tcctaaaata ttattctgtt ctatctgtat ctctttcatt gatttatctt
     8281 cacattctag actatcaatc ttaatttcaa ttccatataa tttttcattt gctatggata
     8341 cctcgtcctt aattccattc atatcttcat taatgttatt tattttattt tgcataaagt
     8401 ttacttctga tttaacttcg gaaacatctt ctttcattac agttatttct tgtctcactt
     8461 ctgacatgtc ttttttcatt acatttatct cttgttttac ctctgatgta tcttctttca
     8521 ttacggtcat ttcttgtctt acttcggaca tatcttcttt cattatattt atctcttgtc
     8581 ttacttcgga catatcttct tttaatacat ttatctcctg ttttacttcc gacatatctt
     8641 ctttcattac agtcatttct tgtcttactt ccgacatatc ctctttcatt gtatttatct
     8701 cttgtcttac ctcggacata tcctctttca ttatatttat ctcttgtctt acctcggaca
     8761 tatcttcttt cattacaatc atttcctgtt ttacttctga catatctcct tttaatacat
     8821 ttatttcctg ttttacttcc gacatatctt ctttcattac agtcatttct tgtttcattt
     8881 ccgacatatc ttcttttaat acatttattt cctgttttac ttccgacatg tcttctttca
     8941 ttacagtcat ttcttgtttt acttccgaca tatcttcttt taacacattt atttcttgtt
     9001 ttacttccga catatcattt tttatgctaa ctacatcttc atttatagta tataataact
     9061 ctaaaatctt ctcttgcact tgcatcacca tccctattaa ttattataac attttacaca
     9121 aatgctatac aatgttaaca tatatatcag aattattaaa attttaatta aacaaaattg
     9181 attcacttta ttacttcaaa actaattaat atctaaaata actacaaata aacaaaaata
     9241 aggtcaacaa caaaaaaggc ttatctcttt gagatagcct ttcttatttt tatttaaaac
     9301 tttgtttttt ataaaataat ctatacattc agctctgaac atgaaatttt tcttccaagt
     9361 gcttttggtt tacaaactga tatacaaagt ccacaatatc tacactcttc ttcattcaat
     9421 tccatcttat cattcaattt tctcgcttga tatgcacaaa ctctctcgca catcttacat
     9481 ttagtacatt tttcttcatc ataagtaaac tcaaagctaa gtctttcatt aacatctccc
     9541 atatgattat gtactatacc acaagcattt tctatagtat tatatcctaa tctattaaga
     9601 ttagatttta aatcatcatg tattttagat attacttgtg caccttttaa aattactact
     9661 gaacatatac caacaaagtc agcacctgcc ataatcattt ctaaggcatc atctccactt
     9721 acacatccac ctaagccaat tattggtata ttaacttgtt tttttatttc atatattctc
     9781 tgtaaagtta ttggcttgat agtttctcca cttaaatatc ctattccatt tccacctaat
     9841 aaaggttgcc ctgtttctat gtctattcta aatgctggtc ctacagagtc acaagcagtt
     9901 attgcatctg ctcctgcttc ttctaatttt ttagcaaaat cgcctatctc atctatcata
     9961 ggaggaagtt taactattac aggtatattt actctttcct ttgcatcttt aagcattggt
    10021 atcaaatctc tatagtcata agatactaat tcaataaagt cagctcctgc atttgcaact
    10081 ttctctacta attcgctact ttcttcaatt gtatgaccta ttgaagctat acagacagtc
    10141 ccatctcttt tcatttgtgg tatttcaaaa tctatccatt gctctggctc aaaatctgtc
    10201 catttttcat tatttaaaag agcattagct gtatttctaa ccatatgtgg tgctggattt
    10261 atagctcttt cttttcttat agtcttagtc acaactgccc ctgctcctgc atcacttaat
    10321 atcttgcatc cagctgcact atatgttaat ggtcctgaac ctattataag tggtgatttt
    10381 aattctttac ctaaaaaatt atacattatc tgcttcccct tttcatatat atttacattc
    10441 gttaaaattt ccaaaattca caagcaactt ctttcatttc tttaacaata gcttttttat
    10501 ctaaatcaac taactgtttt tcttttaaaa tatgtctacc atctatgtat acatttgata
    10561 taaattcttt tttaccctga actactatct ggtcaaatat attatcaaga gtcacaggag
    10621 ttttaaattc atcttccata actattatat cagccttatt accaactgct attttaccag
    10681 aattttgaag tcctaataca taagcaccat gctctgttgc cattctaaat actaaatttg
    10741 ctggcattac tgatgcatct tcttcaactg atttatgcat taaaaatgca cctctcataa
    10801 cttcaaaaaa atcatttata tatccatcag tacccagtgc cacttttatg ccttttttaa
    10861 tcatttttgg tactggagaa aaacctccac ctacttcaca gttactaaga ggcatatgaa
    10921 ccacttttac gcctttttct tttaatatat ctatttcttc atcatttact tttacacact
    10981 gagatgctaa tacagtttca tccaatatat ctaaatcatt atagtagtca actgcttttt
    11041 tgccaaagtg tttttcagca tagtttggct catatatact ctcacataaa tgaaattgca
    11101 ttggtgcatt taattttttt gcatcttctt tagctttctt aataaatcta tcagaacatg
    11161 tgaagcttgt atgtgtacac attattccat ttattaattt actattttct ctactccact
    11221 ttattaaatt agaattttca tctaaacatc taagtccatt ttcaaagctt attctttcac
    11281 aactttctaa ggataaaatc gctttcatgc ctatttcttc tagtattttt ccttgctcaa
    11341 ttaaagttcc ttcttctgta tttggagctt ctagggtatc acaaaaagct accacaccag
    11401 attctataag ttctattgct gatgctttag ttgttgcctt cacttctttt aatccaattc
    11461 tattttctat aaatggccac caatagtcat ttaaaaaacc ttcaaagtca gtaaaatgta
    11521 catttgccgg tattccccta gaaagaactc catattgatg catatgacca ttgacgaatc
    11581 ctggcattat tatagcatct gatttatcta ttatttcttc aatattttta tattttattt
    11641 caacttcttc atttggcaat atatctttta taatgtcatt ttctactaca acagcagaat
    11701 ttgtatatat ttcatcagca gaagatatta agtatttact tttaatagca atcatttatt
    11761 tacctccctg atttaatttt ttcatggcca tataaacttt ctcagaagtt ataggaagtt
    11821 ctcttatacg aactcctgtg gcattgtata ttgcatttgc tattgctggt gcaggtgtat
    11881 gaataactat ttctcctaaa gatttagctc catatggacc tgttggctca taattatcta
    11941 caaaatcaac aattatgttt cctatatctt tttttgttgg aactttgtat tgtaatagat
    12001 tagaagtttg aagctttcca tcagaaccat gtctagggtc ttcatacatt gcaagcccta
    12061 taccttgagt tacaccacct tcaacttgta ttctagcaag tgcaggattc ataactgtac
    12121 cacaatctgt agaacaagca taatttatta ctttaaactc ccctgttgtt ttatctaact
    12181 ctatttcagc aaatcctgct aagaatggtt tagcactctc ttgtatacca taagattcag
    12241 atgaatataa aacttcttga tttccaccac caacagattc ttttcctaat tcacttaaca
    12301 aaacaaactt ctctacatct tcactataac aaactctgtc ttcatatagt attaaatcat
    12361 ctattggtaa atcgagtttc ttctgtgcta cttttaatat cttttgtttt aaactttcag
    12421 cagattttat tgtagcattt ccagttacat aagtagtaca agaagcgtat gcaccagtat
    12481 catatggaca catatctgta tctcctgtat aaacggaaat tctatcaata gatgtattca
    12541 atgcttttgc agctatttgt gcaagtattg tgtcagaacc agttcctaag tctgaagacc
    12601 cagaaaacaa cttataagtt ccatcttcat ccatcctcat atttactatt gccatgtcta
    12661 tgttggctat accagaacca tgagttccca cagacattcc aacagccctc accttattgt
    12721 ttccaatatc cctaacaggg tacttttcat cccatccaat aagtttttta cctctaatta
    12781 tacattcatc aagtgcacaa cttcttattg gaaaattcat tattccgcct tcagtttgtt
    12841 tttttataat attctttaac tttatttctg ttgggtccat attaagttta tgcgctaatt
    12901 catcaattgc acaatcaagt gcaaatgtac cttgtgttgc tccatatcct cttaatgctc
    12961 ctcctggaac taaattagta tatacagttt tcccatcaaa ttttattgca ggtacattat
    13021 tatatgttgg aagtacatta tgacctgatt cagagcaaac tgaaggtcca ttatcgccat
    13081 aagcgcctgt attgttcaat gctttcatat ctatagcttt tatgtttcct tctttatctg
    13141 ccccaatttt tatatcaaaa aacatctgat gtctagtatt agtcattgca aaagtttctt
    13201 ctctagtata aattagtttt gctggtcttt ttgtcatcca agttacaaat gatgcatata
    13261 tctctgttac agctatgttt tttccaccaa aacctccacc tattcttggt tttattactc
    13321 tcacttttga aagaggtatt cccagtgctt tagaaacctg tcttctcata tgataagctg
    13381 attggtttgc agaagttact actaatctgc cattcgtatc tatatacgta taagctctat
    13441 gagtttccat catacaatgg gcttgtgatt gagtcatata agaatgttct actattacat
    13501 cactttcttt aaactctttt tctacatccc ctttacccat tttttctctc gatacaatat
    13561 tttttgtagc atccaatcca aaatcaaatg gacaaaacaa atcatcttct tcatggatta
    13621 aaattttatt tccttccgat tctcttgcat ccaatatagg agtcattact tcatattcaa
    13681 cttttataag tttcatagct ttaatagcag ccctttcatc ttcagcggct attatagcaa
    13741 cttcatctcc tacatatcta actacgtctt caagtattaa ttggtcatga ggtgatgctt
    13801 ctggatatga ctctccaacc attgaatatc tataatttgg aacatctttg taagtgaata
    13861 tgtttactac accttttacc tttaatgcat tttttatatc tatgtttttt atcttcgcaa
    13921 aagcatgtgg acttctaaga agcttaattg ttaaaacatc tttatgaaaa attaaatctt
    13981 ctgtatatat tggttttcca gtaacaatgg cttcactatc tatttttctt acctctttat
    14041 ttaccatcac actcacctcc aatactatta tacattctca ctactaaggc ttttgtcata
    14101 tcttttctgt attctttact tcctcttata ttacttccaa atgaaagttc ttcacttgat
    14161 acttcaccag ctttttctat atcattttct aaacttaata tttcacttgc tttataggca
    14221 atttttgctc ttcttggtct agcgcctatc gcaattttat aaatacttcc ttcttttaac
    14281 attgctccat ttaatactgc aaaatcacct gtacattttc ttatagaatc aaatacacct
    14341 atagcatctt ttttaggtaa tataacttct attaatatat ctttttcaag ctcattttct
    14401 aaaaaatcag ctaagttcat aacccctttt ttatataact taacctttgc atctaccact
    14461 agtaatgaag gtattaaatc tgaaaatcca tacttagcaa atacgctagc tcctactcta
    14521 gctccacttc taaattgaac tcctactata ttagaaacag cgtttgaaat taccccatta
    14581 cagtaatttt taataacctt atttaattct aaagttctta aacttgtatc tgctccaatt
    14641 aatatgctat catcagtttc atttatataa tctaaagcta agtcttttaa atctattgct
    14701 gaaccaatat ttttactacc tagttttaaa aatccacatc ctccaagtat tatgttgttt
    14761 tttcttgata ttagcagttc ataagcttca tctaaacttt ttggaactac atattccttt
    14821 atagttatca tactctctcc ccatatcaac ttaattttca cttaaaagtc taagtgttta
    14881 aatatttatg tattgctctt aattgacctt tatatcctgt acatctacaa aggttaccat
    14941 ttacataatc tataacttct tcttctgttg gattttgaag ctctcttttc attgccaaga
    15001 ccatcattat aaatccagga gcacaaaatc cacactggtc agccccttcg tctgcaagaa
    15061 aatctccaaa cttctttgct tcatccaaga caccctcaag tgttgtaata tgctgacctt
    15121 ctactctagc agtaagataa ttacatgaaa gcacggcctt gtcatttacc atcactgtac
    15181 ataacccaca tgagcctgta tcacaccctt ttttaacact tacatatcca ttttctctta
    15241 atgtatctgc taaaaaatca tctggtctta tacttaattc tttttcttta ttatttatgg
    15301 tacatttgat aatcaaataa atcacctcaa aactcatatt tttaagctaa agttttacta
    15361 tactttttta tgaataaatt acatttatta ataaaggcta ttccatttat gaccttccat
    15421 tctatttttt atagttcctc ttatacaaag tctctcagct gtatcttgag catcttttaa
    15481 tgctttctct tcattagctg tcaatatttt tgaatcttcc attattatat ttccatctac
    15541 tataacactc tctatatttt tcatactaga agaatatact aatgtagata ccggattatg
    15601 cataggtatc gcttttggtg aaagcattgg attaaatatt agtaagtctg cttttttacc
    15661 tatctccaat gaacctatct catcttccat tccaatagct cttgctccat ctatagttgc
    15721 caactcaaga actttttctg ccgacattgc taatgggtca cacttattaa ctttatgttg
    15781 aagtgctgtt aatttcataa gttcaagcat atcttgagag ttattactag cggctccatc
    15841 tactcctaaa ctaactgtta ttcctttttt aagcatttct ggcactggtg caacacctga
    15901 agataagtac atattacttg ctgtattatg agacactttc atatcgtatt ttttagtaag
    15961 ttccatatct ttttcagtta gatacacaca atgtaccata agcacatttg gtccaagtat
    16021 tcctaatttt tctaaaacat ctatgtcata ttgcccatgc aattcttttg ctgcttctct
    16081 atcaaaagga gtctctgata tatgtactgt aaataaagca tcatcatatt cttttactac
    16141 tctccatagc atttcaagca tttcttgaga atttgaccat attgctgcag gagctactcc
    16201 tattttgatt cttccatttt ctgtattatg gtgtttttca aacaatcttc ttacatcttt
    16261 ttcaaccgtt tctacatctt gcattattcc aggatgaact ccaaattgag ctcctgtatt
    16321 catacatcct cttccaagta ttcctcttat tcctaattct ttataagcat ctattattcc
    16381 atcacaaaga cctggcttac tatgaggata catgtagtca accattgttg ttataccact
    16441 tctaagtcct tcaatacatc caagcattgc agcatcataa gtatctttgg gctctaaata
    16501 gttagctgct ggaaatgtca tagtttctaa ccagtcttta agtaccatat catctcccaa
    16561 tccttttaat aaagtttgga ataaatgatt atgtgtattt ataaaacctg ggaataaaac
    16621 tttaccttta gcatctataa tttttttaac atcagtatat ttactttcta tctctttaga
    16681 atttccaata tcagcaatct tgttatcttt tactactaat gccccatcaa aaataacttc
    16741 tctctcttta ttcacagtta ctattatggc attcttaata agtatatcca tcattcttat
    16801 ttccccccgc attatttagt aatttttctt ttaatatatt gacctgttgg atgttcttta
    16861 acattacaac tttgtactac atgatttcct ctcaaaaata catcttcaac tttacattta
    16921 accttaaatc cttcatatgg agtataatct acattttgtt tttgagtttt ataactaatt
    16981 gtttcttctt tattgatatt taaaatgact atatctgcat cacttcctac agctatctca
    17041 ccttttttag gatacattcc aaatattttt gcagcattcg tagaagtaac ttccacaaac
    17101 ttatttacac ttattctatt ttctaagacc ccatatgtat ataaaagtgc taacctatgt
    17161 tcaacaccag gtgctccatt tggtatctta ctgaaatcat caattcccaa gtctttttgc
    17221 cccttaaaat taaatgaaca gtggtctgtg ccaactgttt gaatatctcc actagacaat
    17281 ccttcccaaa ggtaatttat gtcttctttt tttcttaaag gaggagacat tacatactta
    17341 gcaccctcaa atttatcctt attatataaa ttgtcttcta ataatagata ctgtgggcat
    17401 gtttctacta tcatatctat atttttttct ctagctattt ttacagtttc aagtgactcc
    17461 ctacaactta agtggactac ataggatttt gaattagata atttagttat atcagcaagt
    17521 cttgaaacag actctttctc aactatgtta ggtcttgtaa gtggatgata ttttggctcc
    17581 aaattaccat tttgtatatt ttcttctatc aatacatcta gtaaatcccc attttcacaa
    17641 tgaaatccaa ctatacaacc taaatccctt gctttcttta aaaccctgta tattgttcca
    17701 tcatctactt tcatgccatc atatgccata tacatcttaa aagatactat cccatctgaa
    17761 ataagttttt ccatatgatt aaatgtatct ttatttaaac atgtaattgt catatgaaaa
    17821 ccataatcac aataacaatt tccacttgct tttttatgat atgcttctac accttgcagt
    17881 agattttccc cctcattggc ttctgcaaaa tctagtattg tagtagttcc tccagctata
    17941 gctgctcttg ttcctgtttc aaaattatct gcagttgtaa tagaacctgc attcatatca
    18001 aaatgagtat gtgtgtctat tccaccagga attacaattt tacctgtagc atcaataacc
    18061 ttatcaccat ttatttctaa gttatttcct atttcaacta tcttttcatc ttctatcctt
    18121 aaatcactta tataagtctt atcagaggta attatagttc cacctcttag tattgttccc
    18181 atatatacct cctaactttt aagctttaaa gtattttcta agtattgtaa aactttattt
    18241 aaaattgtat tttctaaagg tgctatattc agcacctcta gactatattt aaataattta
    18301 ttgtgaacta aatattattt tccgtataca gattttaata ttccatagta accttctgtt
    18361 cctataaata actgttcttg ctctatatac tcatctattg tgtgagcaag attttcctta
    18421 gaaggaccaa atcctatagt ttttattcca gcttcacctg catagtgact tccattcgta
    18481 cagaatgagt attgagttat ttctgaatct attccagctt cttttaatcc cttatacgct
    18541 gcttgaacaa attcatcttc ttcgtcatat aaccaacctg ggaagaatct ttcactttct
    18601 atagtatttc ctgtatagca atcagctttt tcaac
//
LOCUS       DAMJZE010000072        18630 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJZE010000072 DAMJZE010000000
VERSION     DAMJZE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.072"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(166..1527)
                     /gene="rlmD"
                     /locus_tag="PC541_002082"
     CDS             complement(166..1527)
                     /gene="rlmD"
                     /locus_tag="PC541_002082"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2839548.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            complement(1554..2276)
                     /locus_tag="PC541_002083"
     CDS             complement(1554..2276)
                     /locus_tag="PC541_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2839549.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(2276..2605)
                     /locus_tag="PC541_002084"
     CDS             complement(2276..2605)
                     /locus_tag="PC541_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2839550.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            2935..3861
                     /gene="fba"
                     /locus_tag="PC541_002085"
     CDS             2935..3861
                     /gene="fba"
                     /locus_tag="PC541_002085"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2839551.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(3996..5627)
                     /locus_tag="PC541_002086"
     CDS             complement(3996..5627)
                     /locus_tag="PC541_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839552.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(6110..7147)
                     /locus_tag="PC541_002087"
     CDS             complement(6110..7147)
                     /locus_tag="PC541_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2839553.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(7166..7951)
                     /locus_tag="PC541_002088"
     CDS             complement(7166..7951)
                     /locus_tag="PC541_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2839554.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(7984..9117)
                     /locus_tag="PC541_002089"
     CDS             complement(7984..9117)
                     /locus_tag="PC541_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2839555.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(9165..10292)
                     /gene="hadC"
                     /locus_tag="PC541_002090"
     CDS             complement(9165..10292)
                     /gene="hadC"
                     /locus_tag="PC541_002090"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2839556.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(10292..11518)
                     /gene="hadB"
                     /locus_tag="PC541_002091"
     CDS             complement(10292..11518)
                     /gene="hadB"
                     /locus_tag="PC541_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2839557.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(11533..12333)
                     /gene="hadI"
                     /locus_tag="PC541_002092"
     CDS             complement(11533..12333)
                     /gene="hadI"
                     /locus_tag="PC541_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2839558.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(12372..13571)
                     /gene="hadA"
                     /locus_tag="PC541_002093"
     CDS             complement(12372..13571)
                     /gene="hadA"
                     /locus_tag="PC541_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2839559.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            14248..15246
                     /locus_tag="PC541_002094"
     CDS             14248..15246
                     /locus_tag="PC541_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2839560.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15608..16135
                     /locus_tag="PC541_002095"
     CDS             15608..16135
                     /locus_tag="PC541_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2839561.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            16162..16758
                     /locus_tag="PC541_002096"
     CDS             16162..16758
                     /locus_tag="PC541_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2839562.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16823..18406)
                     /gene="asnB"
                     /locus_tag="PC541_002097"
     CDS             complement(16823..18406)
                     /gene="asnB"
                     /locus_tag="PC541_002097"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2839563.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5786 a         3424 c         2289 g         7131 t
ORIGIN      
        1 tctatttcta taattaaaac tttttttatt tcgtattccc acactccata tatataacta
       61 cagacacatc aaaacacctt ttcaattctt tattcctaat tttattaccc tctaatctaa
      121 atattatatg tctatgaaac tgtctaaagc tcaagatttc tagcattatt ttcgacgtag
      181 caacactaca gtctcacaat gtggcgtatt tgggaacata tccatacact taaccatctt
      241 aatctcataa ccatacccct taaaatcaac caaatcaaca accaaagact taggattaca
      301 agaaatataa acaatctcct tagcaccaaa tccacaaata tccctaatag catccttatg
      361 aatgcctggt ctaggagggt caacaataat caaatcaggc ttgtccttta aatccttaac
      421 aaccttagcc acatccccag caataaactc acaattagta agtccattca acttagcatt
      481 ctcattagca gcaacaacag cttcctcaac tatctcaata ccatacactt tcttagcagc
      541 accagccata acctgtccaa tagacccagt accactatac aagtcaaata caactttatc
      601 attgtaatct ccaataaaat ccctagcaat actataaaga acctctgctc ccttagtatt
      661 ggtttggaaa aacgaaaatg gtgaaatctt aaacttaaga cctaaaattt cttcttgtat
      721 ataatctctt ccatacaata ctcttaactc atcacattgg actgcatctg ctaaaccatc
      781 attaatagta tgaagtacac ctactaactc agcttttaaa tcaatcccta aaagcatatc
      841 tttaaactca tacatatcaa aatcttcttg agaagaagtt actatattaa ccataatctc
      901 attagtatga atcccttttc taactacaag atgtctcaaa tatcctttgt gattcattgt
      961 tctataataa ggaagctttt tttcattgaa aaactctaca ctagaaacta atatctttga
     1021 gaagtcttca tcaaccaaca tacactcctc aacagtttgt atatctatat gctttccttt
     1081 tttatgaaga cctaatgtca aaggtccatt ttttacttca tctccaaaag tatattccat
     1141 cttatttcta taatatttgt tttctggact tccttctatc cctaaaaact gaaatccaaa
     1201 taaatcttgc tttaaaaata aatccataac ttgtttttct tttatttcta attgtttttc
     1261 atatggcaca gacaatagtg tacatcctcc acactctcta aagtgcttgc aagtttcttc
     1321 agtttcaagt ggtgagttct ctaataattc tatcatctta acttcagctt tcttacttct
     1381 aactttttta actccagctc tcaccttttg accacttatt ccacccttca tatggataac
     1441 tctatctcca acttggctta aagatgtacc accaaattcc attttatcta cttcaaattc
     1501 tataacatct tttttcttca cagacattcc tccaatttac tatagtatct ttattatctt
     1561 tcttcaatca attttacttc ttcatctgtt aactctctat actcacctaa ctctaaactt
     1621 tcatccaatt ttaaattgcc catagacaat ctttttaagt atactacttt tttgcctacg
     1681 ctttcaaaca ttctttttac ttggtgaaac ttaccttcat gtattgtaag ctctatttct
     1741 gattcatcat cactttttaa tatatttaac tgtgatggca tagttttata tccgtcatca
     1801 agcactacac cttctaaaaa agcttctaca tcttcttcag taactactcc atcaattttt
     1861 gcataataag tcttaggcac atgcttctta ggagataaaa ctctatgaga aagctttcca
     1921 tcattagtta aaactaataa tccctctgtg tctttatcta accttccaac aggaaatggt
     1981 tcaaaagcta agtatgataa atcaattaaa tctaatactg ttgggtcata tttatctgta
     2041 gttgctgata tatatccatc aggtttattc atcatcaaat atatatattc tctatatata
     2101 acttcttctc cattaaataa tatctcatca ttttcagtgt ctacttgagt tcctggattt
     2161 gaaacttcac ttccatttac tactacagac ccttgtttac aatacttctt tatttcactt
     2221 ctacttccat aacctaaatt tgaaagaatt ttatctatac gttgcttttt agccattaaa
     2281 aatgctctcc attctcataa tatttttctt tttcaaatac tgtaaatgct aaatctatac
     2341 tttctacatc taaattattt cttatactat cagttattat tcttgctact atatcttgta
     2401 cttcttgtcc tctatcaaac caagctactt caacaaaagg atatacatct gctattttac
     2461 catctcttat tgcaactgac tttatacatt ctatttcaaa gtagtctctt ggacatttta
     2521 cagcttcaac taaatcattt atcatttttt cacttatttt acatatatca ttttcattaa
     2581 ttcctcttat ttttatctgt ggcatagttt atctccttta ttttaaattt ttagtgaatt
     2641 atcatccact taattccttt cgataacgtt tgaagtggta acttcttggt caaggcaact
     2701 attgttatca gtatctccaa actaataagg tagttcctac ccttatagtt agcattatat
     2761 agctagtttt cctaaattgt ttatattctt tattatttta tttaatccta ttgtcttcta
     2821 tttaacatta tacttgtaat ttatagttat tacaatttac tatatacttt ttttgattta
     2881 acgttataat ataagtgatg ggtaaatttt aaaagttaat aggaggagtt taaaatggca
     2941 ttaattacta caaaagaaat gtttaaaaaa gcttacgaag gtggatttgc tataggtgca
     3001 tttaacataa gtgacttaga acaattacaa ggtgttttaa aagctgctaa agctaaaaat
     3061 tcttatgtta tgatacaagc ttctatgtca gctgttaaat atgctggacc tcatacatta
     3121 gttgaaatgg ttaaggctgc ttctgatgaa ataggtgtag atgtagctct tcacttagac
     3181 catggtccaa atatggatgc tattaaaact tgtattgatg ctggtttctc ttcagttatg
     3241 atagatggtt ctcattttga ctttgaagaa aatgttagaa taactaaaga agctgttgaa
     3301 tatgcacact ctaaaggtgt tgttgttgaa gcagaacttg gtgttctagc tggaacagaa
     3361 gatgatgtta cttctgatgt tcacaagtat actcaaccag ctgaggcagt tgagttcgtt
     3421 gaaagaacag gtgttgattc attagcaata gctattggaa cttctcatgg tgctttcaaa
     3481 ttcaaaggag aagctaaatt aagattcgat atactagaag aaatccaaag caaattacca
     3541 ggtttcccaa tagttcttca tggtgcatct gctgttgacc aaaacgctgt tgctacatgt
     3601 aatgaatttg gtggaaatat agctggtgct aaaggtgtac ctgtagatat gttaagaaaa
     3661 gcatcttcta tggctgtttg taaaataaac atggatactg acttaagact agctatgact
     3721 gctgctatca gaaaattctt agctgaaaat ccaaaagaat ttgacccaag aaaatacatt
     3781 ggtgctggta gagatgctat acaagctgta gttgaaagca agatagatga tgttttaggt
     3841 tctgctaact ctataaacta atataacttt taaatattta taaaagaaaa gagatgactt
     3901 aaagtaatta aatttatttt taagccatct cttttaagtt gtataaatta tgtatcttat
     3961 aatattttaa ttatattgtc tttttaaact gacagctaaa cgtgaatatg tccactcttt
     4021 atctttctat ggatggtaga aggatatata cctataactt cagcagcctt tgttattgaa
     4081 ccatacttat ttatagcctt acttatcatt atttggtcca ctattttata tgcatcatta
     4141 agattcataa ttccatttac cactattggt gataaatcat ctgtttcatt atcaatcata
     4201 tctaaattta tcagcatgtt aaattcatct tctccaatta catttttagc tgataatact
     4261 acaaatcttt caatgatgtt ttttaattct cttatgtttc ctggccaaga atattcttct
     4321 aataactcaa taactttagg cgaaacttct acttctctat tgtacttttc attgtaaagt
     4381 tttaagaaat aatttactaa tggtactata tcctcttttc tttctctaag aggaggtatt
     4441 tttactggaa taacacttaa tctatagtac aaatcttgtc taaatttaag gttatttcta
     4501 aggtcttcat gagaaatatt agtagcactt atatatctaa catctatttt tataggctta
     4561 ctaccaccta ttctagtaat agtattttct tgtataaccc taagtaattt tttttgcatc
     4621 tgcattggta actcacctat ctcatccaag aaaatagttc caccattcgc ctcttcaaaa
     4681 agtccctttt tacctttttt cttagctcca gtaaaagaac cttcttcata tccaaaaaac
     4741 tctgactcta ataattcatt tggtatagct ccacagttta tagctataaa aggtttgtct
     4801 ctccttgaac tatacttata tattgttctt gctattattt cttttcctac acctgattct
     4861 ccatttataa atatagaaga atctgaggct gcagctctct taacaacttt ttttatattg
     4921 tccataatct tactttgact tatatactct gtgtcttctg atatatcact atattttatc
     4981 tgagattcaa gcttaataac ccttgaaata tctctccctg ttagtactcc tcctgtaagc
     5041 tttcccttag aatcccatct tggaactgcc gtatatgcaa taagcttccc atctgggtaa
     5101 acgatatttc tataaatctt tttcttcact ttaagcatgt ttaatattat tggttcttca
     5161 actaatcctt gtctcacaag gtctgttata tttctaccaa gtacctcagt tcttttaaaa
     5221 ggtaatatct catcactgac agtgttcatc ttttcaatta ccccatcttt gttaaacaca
     5281 acaacaactt catctatatg ctctattata tcttcaatta ctttatcaga tattagagac
     5341 ttctcaaata aataaactat attattttca ttattattat ctttcatatt gtatatatgt
     5401 gcagtcatcc ttaactctct gaaagctatt ttctttttac tatctgtcag tatttctgtt
     5461 ttgtcaaaat ttgaatctaa ttcatttata tttctaggct tttttatatc tagaaaacta
     5521 aaaatactat ctattagatg attacagtat attatttcgc ctgattcttt acataaaatg
     5581 gctggatttt ctacatgttc tataagccct attaaattat tatccattaa tattcgcacc
     5641 tttcattaag taatttattg aactacatac aaaatttatt gtttgcttac aataaatacc
     5701 atcagcacat ataatcaact ccttataata ttcgtctttt caagtagttt atttcattaa
     5761 aacgtaacta agatataatt ttaatgatta tagtttgtta aaaatttcat tttgctttta
     5821 attgtatttt atcacatatt tagtaattat ttgtctattt aatacaattt aattaaattt
     5881 aagatttatt taaaaaaaca aagataatcc ttacagaata taactcacaa tttgtaagca
     5941 atatacatat aaggattatc cagattatct tgtattataa aattttattc aaacagactt
     6001 tttaataact gacaaataca cactagcaat aacttatcaa agggttaata attatgttac
     6061 taactaataa gacttattca atcagtaata tttatatagt ttgtatctat taagctttta
     6121 tagcttctaa taaatcatca acgctatcta atttttctat aagcattgga actatttcat
     6181 gtaagtctcc aactactcca tagtcagcga tttctaaaat tggagctgct gggtttttgt
     6241 ttatagcaat tatgaaatct gatgattgca tacctgctaa atgttgtatt gctcctgata
     6301 taccacaagc tatatatagg tttggtttaa cagtagttcc tgtttgacct acttggtgag
     6361 aatggtctat ccatccagca tcaacagccg cacgagaaga accaactact ccacctaatt
     6421 tgtcagctaa tttcttaagc atttcaaatc cttctggtcc acctaatcct agaccacctg
     6481 atactataac atttgcatct gtaagagata ctaaatcttt cttagtttta actgtttcta
     6541 aaacagtagt tcttatatca tcttgagtta ttttgtagtc taatgctata acttcaccag
     6601 ttcttgtttc gtctttttca gctttatcca taacaccagg tctaactgta gacatttgag
     6661 gtctatggtt tggacaaatg attgtagcca ttatgtttcc accaaatgct ggacgagttt
     6721 gttttatttt cttatcttct gggtctattt ctaacttagt acagtcagct gttaatccag
     6781 ttccaactct tgaagctatt ctaggtgcta agtctctacc tatatgagtt gctccgaaaa
     6841 gcattatttc tggttttatt tcatctattg catcttttat tacttttgta tatgcatcag
     6901 ttgtatattt ttctaaaaga gcatcatctg caacataaac tttgtcagct ccaaaagtga
     6961 ttaattcttt agctaatcca tcaacatctt ttcctaataa tattgcacaa agttctgcat
     7021 ctacttcttt agctaatttt cttccttctc ctaataattc tataactact ggagctattt
     7081 ttccttctct ttgttctgca aatatccata cgtttttata agaacttaaa tcttttatat
     7141 cattcatagt ttgtactcct ccaacttata taatgtgctt ttgttttaga ccgattatta
     7201 ttttactagc tatttcatca gctttacctt ctaagatttc tccttttcct cttggttcag
     7261 gagtaaatga tctaaatact ttagttggtg atgcttttaa accaacttct tctttattta
     7321 catctaaatc atcaattgtc caaactttta catcatgttt atatgctttt actattttat
     7381 caacagacat gtatctaggt gtatttaact cttttactgc tgttaaaagt acaggtgtac
     7441 ttacttttat taattcgtat ccatcttcta attgtctttg aactattata tcttttcctt
     7501 ctattttgaa atcttgtaca tatgtaactt gaggaatgtc taatttctct gcaatttgtg
     7561 gtccaacttg tgcagtatct ccatctattg cttgtcttcc tgcaaatatt atgtcatagt
     7621 cacctacttt agatattccc gctgcaatag tatttgatgt agcccaagta tcagctcctc
     7681 caaatgctct atcacttaat aatattgcat catcagcacc catagctaaa cactctctta
     7741 gcataaagtc tgcttgagga ggtcccattg ttataactgt tatagttaca tcatcatgct
     7801 catctttaat ttttaaagct tcctctaatg catttgcatc atctgggttt aatatacttg
     7861 gaactccatc tcttataaga gtacctgttt ctttatttat tcttacttcg ttagtatctg
     7921 gaacttgttt tacgcaaact aatattttca aagtctatac ctccaatttc attagattat
     7981 ttattatttt aatacgtttg atgaaattac cattttttga acttctgatg ttccttcgta
     8041 tattgatacg attctagcat ctctgtacat tctttcgatt tcataatctt ttataaatcc
     8101 atatccaccg tgaagttgta atgctttata agcaacttca tttgcaattt ctgatgcata
     8161 gtatttagcc atagcagatt cttgagcaac tctcattcct gcatctctct tttgtgctgc
     8221 atcataaact aatcctctag ctgcacaaac ttttgtttcc atgtcagcta tagtaaattg
     8281 agtattttgg aatttagcta taggcttacc aaattgaact ctttgttttg tatatttaac
     8341 agcttcatct aaagcacctt gagctattcc tagagctaag gcagcaacac ctattctacc
     8401 aacttctaga gtacccattg ctatcttaaa tccttgacct tcttttccaa gtaagttttc
     8461 ttttggaact ttaacgtttt caaatattaa gtcagaagtt tcagttcctc ttatacccat
     8521 cttatcttca tgagctcctg ttgagaaacc atcccattta ctttctacta taaacgctga
     8581 tgttcctcta agtcctttac ttctatctgt aactgctatt actatagcta catcacagaa
     8641 tggtccatta gttatgaaag tttttctacc atttaatata tagtggtctc caactaattc
     8701 tgctgttgtt tgttgtccac ctgcatcaga accagctcct ggctcagtta aaccaaatgc
     8761 tcctattaat tcacctgttg ctataccttt taagtatttt tgtttttgct cttcagtacc
     8821 attgtaaagt attggtaatg aagcaagaga agaaccagct gtaacaaaag tagctgttga
     8881 agcacatctt ctagctattt cttccattac tatagcgtaa gaaacatatc ctgctccccc
     8941 accaccatat tcttctggga tattactgct tataaatttt gcatcagcta attttttgat
     9001 taattcctta ggcattacac ctgttttatc catttctgct ggtaaagtat ctaattcttt
     9061 actaacaaaa tctcttactg cttttcttaa aagttcttgt tctttgttat ataacatagc
     9121 ttcctccaat tttcatattt tatagtaaat ttattttaat gaacttacgc taaactcatc
     9181 atctcagcaa aagtttgaat acgagttctg gcttgttcat tattctgagt ttgttggtcg
     9241 atttcaacat ataaagtagg tattccacta tcttctatat cttttctaac taaaggataa
     9301 tcgtattctt ctgggtcaca gaatttcatc atacagaaga taacaccatc tatatctttc
     9361 tttttaactt catctactat aagtgaccca cgttttttct tagggtcata agctaatgaa
     9421 catccttcta tgtttgacca ttgtcttgct aatctttcta acgcatcatc acctgctggt
     9481 acatctgttc tgaattgtct tgtttcttgt gctaagtcgt cagctacaac tgatatattg
     9541 ttgtcttcta aaatgtctaa tatatcttta gaatcagcta atatacctgt taataaaact
     9601 ttctttccag aacagacttc ttctggcata gcatttaatt ttgctattaa atctttcact
     9661 agctcagtat gttcttcttt tctcataaag aacccactct taattactaa gcttcttatt
     9721 gatggtttta tagtattaga atgtttataa gctacttcaa caaagtctct catagttttt
     9781 ctatgttcat tgtaaacttc tatactttca tgaatttttg cttcttctat ttcatatcca
     9841 caaatttctt caagttctct ttttacgcct ttgtactcac tgattaagta ttttacacca
     9901 gcttcaagtt ttctattttg tgggtgtact aatgatatat attttatatg aggtactgct
     9961 gatttccagt tttgtcctaa acaaattagt gtatcacaca tacctggtat aataactcca
    10021 gataattcat cataagcacc ttttaatcca tattctaaac atgattgcat tattgaacat
    10081 gcaaatgcag ggaaatattg tttagctaaa tctaattctg tttgccctcc ccatatacca
    10141 actggaagca ttccagctgc atgtataatt tcttctgggc aataaactgg gaaacaacct
    10201 atagcttttt taccagtttc acttttatat ttttttacag ccgcatttgg attttcaact
    10261 acttctttca ttttagataa aatagcttcc attatatctc acctctatta agtttttttc
    10321 tttcttccat tacttcaact aaaccttgaa ttcttgtttc aaattgagca ttagtgaaag
    10381 ctctagggtc tgcttggtca ccatcaaatc cagcatatgg taatccagtt tcttcagctg
    10441 ctcttctttg catttcatat tgtattaaac tcataagttt acagcttctg ttcatatgat
    10501 agaatgctcc atcacattta ccctctacta aagaatcaac tctatatttt gtcatacggt
    10561 ctaggtttac attgttaaac atagtactat atgctactgc cattccatct aaatcattaa
    10621 cttcatattg taatgcccaa gcatgtgggt aaacactacc tgtcatgtta actccaaatt
    10681 tagctaatgt tttcatcttg tatcctatat atggccaaca aggtatacct tccatcatta
    10741 ttctgtattt ttcttcccct ctgaaagaag atttaccagt tttcatgttg tcctctaatt
    10801 cttctataag taacttaaat gcttctgtag tttctttttt acctctagca caaactatta
    10861 cagccatgta agtaaataag tcaaatccat tcattggaga aggagaagaa tctgctggta
    10921 aactcataga atacttccat agtcttccgt tttcagctga tattttcatt acttcttcaa
    10981 acttcttagg gtcaaatttc tttcctgata taatttctag ttgttttata gcttcttcaa
    11041 attgagcttt aatataatct attcttgatt gagtaacttc gtcttcatta ttgaaagttg
    11101 tatcaatcat tattaaaggt atatctaatt ctcttgaaat attttcatac cattttataa
    11161 cttggttaca tatattgtta cagcaaagta ggaaatctgg agctggcata tccaaagctt
    11221 cacatccacc attttctaaa agaccaaaat ttgttcttgc atatgcacat aggtcaatag
    11281 aatatccttt agattcagct atttcacata attctaaaga acctttttta gctgctactc
    11341 cagctgcttg gttttctgga tataatacgt ttaagtcaaa tacttctgct aactcttgag
    11401 gaaatactga tgttgaccaa cctacaggtc ttccttcttc tttagcttta aatgcatttg
    11461 catattgttc agctaataaa tcattaatta ctactctagc ttcttttttt tcagacattt
    11521 tcatccctct ttttatatat ttttcacttc tttttgtgat tcttttgctt catcaaaagc
    11581 atataacgct gctcctaaag caccagttaa ttgaggtata tcaggtacaa taatttctgt
    11641 gttgatttct cttgccatag ctcttacaat accactattt ctagcaactc caccaaccat
    11701 aactacattt ctttgtactc ctattctttt tactaggcta gaaactctct ttgctactga
    11761 agtatgaata cctgccacta tatcttcaat ttttgcattt tcagataaat gtgatataac
    11821 ctcagactct gcaaatactg tacatgtact gcttattgat acttcatttt gagaattcat
    11881 agatatactt ccgagttcag atacatcaac ctctattatt tttgccatta catctaaaaa
    11941 tcttcctgta cctgcagcac atttgtcatt cataagaaag tttaatagtc ttccattatt
    12001 atctaatttt aatacctttg catcttgacc acctatgtca ataatggttc ttgtctctgg
    12061 aattatgaaa tttacccctc tagcatgaca gcttaattca cttatttgct tatcagcatc
    12121 tgaatagttc attcttccat atcctgtaac tacaactttt ttaatatctt ctcttgcaag
    12181 acctgtcttg ccatataatt tttctaaaac tcttgatggt ccagtagtcc cagtaccaga
    12241 ggatattgtt tcagaagcta caatatcttc cccattcttt aagattactc cctttgatgc
    12301 agttgaacct atatctaatc ccattgtgta catctttagc attctccttt taaaaaatct
    12361 agaattacta attaatatct tacaacttta ctatctttaa agttatttat tttctcttca
    12421 tcgtagccta aagattttaa tacttctaca gtatgttgac ctacttttgg tgctggtgta
    12481 tattctttaa ttccttcatt tctaaacata actggagtat taactaagac acctgtattt
    12541 ccgctatcgt atgttttctt aaataagaag tcatttgccc aagcttgttc gtcatctaat
    12601 aaatcttcac agctttgaat tttttcaaat ggtaagtctg cttcttctaa taaagctgac
    12661 cactcgtcta atgttttttc taacatagct tctccaacta tcttaactaa atcttcaaca
    12721 tgattaacca ttgaatctat gttattatat ctatcatctt ctaatatata ttctctattt
    12781 ataaccttac agaatttgcc taaccacttg ttgtattgta ttaaagctaa ttgaatccat
    12841 cttccatctt tacatttata tgtagtcatt aatgggctgt ttggattttc tcttgataaa
    12901 ggcatttcat ttccgtattg tgctgttgtt atcattgttc ccattccata tatagctgta
    12961 tggaaaagac ttactgttac tctctcacct ttaccagttt gagctttttt atgtaatgct
    13021 gctaaacttc ctgctgctag tgctagacct gcatagtggt caccaaatcc tgctgctgta
    13081 tttgctggag atgttccttt ttccataaca gattggctaa ctcctcctct tgcgaagtat
    13141 gcagtatagt caaatcctgg tttatctttt aaaggtcctt tttcaccata tcctaatatt
    13201 tgagagaata ttaatcctgg atacttatct tttatttggt cataagctat acccattttt
    13261 tctaatgctt gaactctaac attagttaca aatatgtctg cttctgataa taatttatgt
    13321 aatatttcta ctccttcttt tgattttaca ttaatactta cacccttttt atttccattt
    13381 tctaattcaa acatagggtt ttcatcatct gatgctggag atttaaatgt tccacccata
    13441 actcttattc catcaccttc tataggttca atcttaataa cctctgcacc ccagtcacct
    13501 aacatttttg cacaacatgg tgctgcgatg aaacttgaaa gttctactac tttaactcct
    13561 tctaaaagca taacgttttc ctccttaata gtatcaatta taaaatttcg attaaatcac
    13621 tcataaaata cttttttctg atattaactg tctgatgtca aagttcctaa tcttatctaa
    13681 aataacttaa aaattattaa aattattttt tattgtttta ttgtcttaca cctatattgt
    13741 taagcattta tcgtgccaac tttatattta atcaaaatat cgcttgattt gaatgttttt
    13801 taacaatatg caattgaata atctttttca tatcgtggca tcttgccact atccattgca
    13861 ttttgctatt ctttctatgc ttataaaagt tttttttata tattttttta tgaaaaagct
    13921 tgacatttca aaaaaagttt tcattgctaa actcaaaaac acatgttatc attgtaatta
    13981 atcagcaagt tagttaacta atttcaaact tagataattt tttaataata tttaatctaa
    14041 aaaataacaa tttctttgat aaattttaga gtaattatta cattaaataa aaaattacta
    14101 aatatcaaaa taacacaggg aaaacaattt caaaactaaa tttttaacct taaaaatttt
    14161 gtataaaaaa tataacctta tatgctacat attatttcta aatgaaaaat taccataata
    14221 atttaataat tacaggaggg tattgatatg aaaatactag tatttggagc acgcgattat
    14281 gaagaaccag taataaaaaa atggtctgaa gaacataagg atgttcaagt ggatatttat
    14341 cctgaaaaca tgactgaaga aaatgtagtt aaagctaaag ggtatgatgg tatatctata
    14401 caacaaacta actatataga taatccttat atttatgaaa ctttaaaaga tgctggggtt
    14461 aaagttatag cttcaagaac tgcaggggtt gacatgatac attttgattt agttaatgaa
    14521 aatggactta tcgttacaaa cgttccttct tattcaccta atgcaatagc tgaattagct
    14581 gttactcaag ctatgaacct tttaagaaag actcctctag taaagaaaaa agtctgtgaa
    14641 ggtgattacc gttggatagc tgaacttctt ggaacagaag ttagatctat tacagttggt
    14701 gttataggta caggaaaaat aggtgctact tctgcaaaat tattcaaagg cctaggagct
    14761 aatgtaattg catttgacca atatccaaat agtgatttaa atgatatatt aacttacaaa
    14821 gattctttag aagaccttct aaaagaagct gaccttataa cattacatac tcctttactt
    14881 gaaggaacaa aacatatgat aaataaagat actctagcta taatgaagga tggagcttac
    14941 atagtaaata ctggccgtgg tggtttaatt aatacagggg atttaataga agcactagag
    15001 tcaggaaaaa ttagagctgc tgcccttgat acatttgaaa ctgaaggatt gttcttaaac
    15061 aaaaaaatga atcctggaga attaactgac ccagaaataa ataaacttct ttctatggaa
    15121 caagttatat tcactcatca ccttggtttc ttcactagta cagcgattga aaatatagtt
    15181 tattctagtt taagtagtgc tgtagaagtt ataaaaacag gaactgctac taatagagta
    15241 aattagtatt ttataaatat attttttcat taattagcta ttatctaaca aaatagcctt
    15301 taatttccca aatataagag agtcagctca gtatatattg agtagactct cttatattat
    15361 tttttatatt aaacttgtac tcttcttatt cataccaaat taaatttata ttaacttatt
    15421 ctaactactc ctactctata cattttacct atttttactt ttctaattat atacatttga
    15481 atgggtacaa tctgtatttt acaaaaaaaa cacctaacaa actatgaaaa ttgtatgatt
    15541 aatattaata tatttataag aaaacataac aattatgtta gctctggatt tttagggagg
    15601 gagtatattg gaaaaaatga ataaagttac ttctaaaaaa actacctata ttgttacttc
    15661 agcattattt gcatccataa tatgcctgac aatagcatat atattacata ttccagttgg
    15721 tggaaacaat ggatatgttc atataggaga tgcttttatt tatctagctg caactatttt
    15781 gccaacaaac tatgctatag ctgcttctgc aattggtgct ggtctagctg atttatctac
    15841 aggtgctgct atctgggtaa taccaactat aatcataaaa ccaatacttg tattattttt
    15901 cacttctaaa agtgacaaga taataaataa gagaaatata gttgcttcgg ttgtagcagg
    15961 tatagtagga ttagttttat acatgtttgc tgaaggtata attattggaa gttttacaag
    16021 tgcatttgta atgagcttac ttggcttatt acagccaata ggaagtttta ttgtttttat
    16081 aatcttaggt atggcacttg ataaacttga ttttaaaaaa cgttatttta attaagaatt
    16141 taaatttatg gagggaaaat tatgaatatc ttatatacaa ttgaaaatag tatctatgta
    16201 aatataacta atacatgtcc atgtagttgt gttttttgta taagaaacga aaaagatgaa
    16261 gttgcaaata gtggtagttt atggttggag catgagccaa gtgtagatga agtaaaagaa
    16321 gccttcaata aatacaattt agacgactat gatgaaatcg tattttgtgg atatggcgaa
    16381 ccattaatga gaataaatga attgatagag atagcaaagt ttataaaaga aaaaagtagc
    16441 ataaagataa gaataaatac aaatggtcta agtgatttaa tacataacaa aaaaacagct
    16501 attctgttaa aaaatgttat agatgcagta tcaataagtc ttaatgcccc aaataaagaa
    16561 gcttataata gagttactca accaaagttt ggagaaaaat catttgatta tatgttagat
    16621 tttgcaagag attgcaaaaa atatataaaa gaagtagctt tttcagtagt agatgaaata
    16681 tctccagaag aaatagaaga atctaaacaa ctggcaaaaa aacttgatat accattaaga
    16741 gttagacata agaattagca agtaaaatta taaaacatta agaatcacct gaatggtatt
    16801 tcaggtgatt cttcctcttg tattaacatt ttacaaaata gatttcatac cacactagga
    16861 atgtgtaaat catccaaatc tttctccaat tatcagagtt tccactaatg tagtcattaa
    16921 aaatctcatt aatctcatct atgttaaaga atttatctgc tatctcacta ttgaatttat
    16981 ctcggatatc cttattatat cggtcatcag ccatccatat acgaattggc acaataaatc
    17041 ctaatttatt tctaaaagca atctcttctg gtaatgtttt tgatgcagct gtacgaaagg
    17101 caattttatt ctcttttcct ttcactttat attctgatgg tattctggat gcaatatcaa
    17161 atattcttgt atctgtaaga ggcattctga cttccaatcc tactgcagta ctcattttat
    17221 ctatattaaa ataaatatct ccttccaacc atatttgaat atctacattt gacatgttag
    17281 taagtgtatc tagttcttct gtttcttcat aaattccttt taccacttca ataggaagca
    17341 catcaggatt gtatttctta agaatttttc ttttctcacc ctcattcata atgtttgtat
    17401 ttccaatata aaatgtcttc aaattctctc cataacactc tgccttatgg tatatattat
    17461 atccaccaaa aaactcatct gctccttctc cagagtaaca gatatttgtg ttttcagctg
    17521 ctgccttgca tccaatagca aaaacgatag cagatgcatc tccagttggt tgctccatat
    17581 ggtacattac ataaggtact gacgcaaaat actcttcagg tgtaattata tatctcgaat
    17641 ttttacaccc aagaatatta gctgtctgtt gtgctaattt tgattcatca aaacgctctt
    17701 cctcataacc acatgaacct gttttttcta tatctgacat tgccaatata taagatgaat
    17761 caacacctcc tgataggaat aactctgcag tttcattctc tgattttacc tctggcataa
    17821 ttttctgaag tgttgtatga atttcatccg tccattcctc aatagacttg ctgtggtcag
    17881 gatgaaattc aggtttccaa taacgaccaa tacatactgt tttattctga tagacaaggt
    17941 aatgtcctgg cataagcttc tttactccac ggaagaatgt attttcacct gctacatatg
    18001 tgaaagacat ataaatctgt aacatgtctt catttaactc tttcacaaat cctggttgct
    18061 ccataatctt acgaatagat gaaccataga gaagcttccc attttcagtc tcataataat
    18121 agaaaggttt taccccaaac tggtctctta ggcagaagag tttcttttcc acagtatccc
    18181 ataatgcaaa agcaaacatt ccatgcatgt gatttgccat atcatatccc catgtcttat
    18241 atgcttccac taaaattgct cgttcacgct catctctaca taatgaagaa tcgattccaa
    18301 gttcttcaca aagaactttc cagtttcgaa tatgcccatt gtattctaga atttttatca
    18361 tatatgtgtc ttttcgtgtt aaatactttt cttctaaatt tatcatatgt gtatcctttc
    18421 gtattaaaac atttaacttc taaatttatt aaatgtttat atatatttat tcacttataa
    18481 tcactgaaaa atcgactttt ttcaaagtaa attctttttg aattagtact aatctgctct
    18541 ttattaatgg cataaatatg attatcaagt attatcagtg ttataacatt gaatttttta
    18601 ttcttcataa atattgcccc ctttatatta
//
LOCUS       DAMJZE010000073        18536 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJZE010000073 DAMJZE010000000
VERSION     DAMJZE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.073"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            141..1301
                     /locus_tag="PC541_002098"
     CDS             141..1301
                     /locus_tag="PC541_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2839564.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1643..1804
                     /locus_tag="PC541_002099"
     CDS             1643..1804
                     /locus_tag="PC541_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839565.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            1997..2626
                     /locus_tag="PC541_002100"
     CDS             1997..2626
                     /locus_tag="PC541_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2839566.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2865..4124
                     /locus_tag="PC541_002101"
     CDS             2865..4124
                     /locus_tag="PC541_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2839567.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4383..5378
                     /locus_tag="PC541_002102"
     CDS             4383..5378
                     /locus_tag="PC541_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2839568.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5624..6691
                     /locus_tag="PC541_002103"
     CDS             5624..6691
                     /locus_tag="PC541_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2839569.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7225..9135
                     /locus_tag="PC541_002104"
     CDS             7225..9135
                     /locus_tag="PC541_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2839570.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9198..9821)
                     /gene="ytaF"
                     /locus_tag="PC541_002105"
     CDS             complement(9198..9821)
                     /gene="ytaF"
                     /locus_tag="PC541_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2839571.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10009..10653
                     /locus_tag="PC541_002106"
     CDS             10009..10653
                     /locus_tag="PC541_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2839572.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10867..11925
                     /locus_tag="PC541_002107"
     CDS             10867..11925
                     /locus_tag="PC541_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2839573.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12029..13213
                     /locus_tag="PC541_002108"
     CDS             12029..13213
                     /locus_tag="PC541_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2839574.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13420..14133
                     /gene="rpsB"
                     /locus_tag="PC541_002109"
     CDS             13420..14133
                     /gene="rpsB"
                     /locus_tag="PC541_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2839575.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14212..15123
                     /locus_tag="PC541_002110"
     CDS             14212..15123
                     /locus_tag="PC541_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2839576.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15208..15912
                     /locus_tag="PC541_002111"
     CDS             15208..15912
                     /locus_tag="PC541_002111"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2839577.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15938..16495
                     /gene="frr"
                     /locus_tag="PC541_002112"
     CDS             15938..16495
                     /gene="frr"
                     /locus_tag="PC541_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2839578.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            16498..16701
                     /locus_tag="PC541_002113"
     CDS             16498..16701
                     /locus_tag="PC541_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839579.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16730..17464
                     /locus_tag="PC541_002114"
     CDS             16730..17464
                     /locus_tag="PC541_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2839580.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17477..18256
                     /locus_tag="PC541_002115"
     CDS             17477..18256
                     /locus_tag="PC541_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2839581.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7618 a         1847 c         3222 g         5849 t
ORIGIN      
        1 tgtcaatagt taggtatata tgtatttaat tagcatgata tagataaaag tatttatttt
       61 aattgtctat tatctgaaaa agtctttact tagaaatcat aaaaatcaaa ttaattagga
      121 aaagaaaggg tgtgtataat gtgattgaga ctggatttgt atattgtagt ttcttggttg
      181 tattcgtagg tgtaattttt tttatggttg ataaattgaa atggaaggta tttgatgtta
      241 ttaatccaat gcttttaata tacttaggtt cagctatact tggatcattt ggtttatttg
      301 cacagaatga agatgttgga acgtatcaaa acataataac agtgaacttc cttccattaa
      361 tgttagtgtt tttgttaatt caatgtgatt taagaaaaat attaaagatg gggcctaaaa
      421 tgttattatc atttttctgt gctacattta catttatgct tggatttgtt atagcattca
      481 taatttttaa aaattcaatg ggtgcagatt catggaaaat tcttggggca gcatctggtt
      541 catggatagg tggctcatca aatatgattg cagcagctaa tgctctagga gttgcagaag
      601 aaggacttag ttatgcaata cttatgggtt ctataggata tacagtatgg atgtctgttt
      661 gtctaatgtc tgtgaaattt ggacctaaat ttaacaaatg gacaaagtgc gatacaagtt
      721 ttatagatga ggttacatca aaacttgaaa aagaaaataa agataataca gcacctacgt
      781 ttgtagaact tatgatttta ctttctgttg ggtttggagt tgcgtctttt gcacaaattg
      841 tatccaagat acttccaact tcaggtatac ttaatgaaac tatgtgggta gtacttgttg
      901 catctacagt atcaatatta ttgggtatga caaaaatatc aaagcttaaa gggagtacac
      961 tggtaggaaa tgtgtttatg tatttactcc ttgcagctac aggtagtaaa gcaaatttct
     1021 ttggattgtc agaagcacct gtttacataa tgtttggatt ggtagtagtc cttgtacatg
     1081 ctatttcatt tatagggctt gctaaactat ttaaacttga tttatttact tgtgaagtag
     1141 gaagtatagc taatatagga ggagtaacta cagctccaat gattgctggg gttcacaatc
     1201 ctgtattaat accagtaggt gtacttatgg ggcttctagg aaatattata gggacttatt
     1261 gtgctcttat ggtaactcaa atattacata tgttagctta ataaaaatat ggtataaaat
     1321 taataattta aataagaaaa gtcatttcaa tttaagcaga agcttttact gaaatggctt
     1381 ttttgtttga caagataatc tatttcaaga agatgttttg acatatttag tgtataatat
     1441 tgtaaaattt actaaaaacg agatatattt aatttttata ttgaatcgaa cgtctgtatg
     1501 atgtataata aatatacaga acgaatgttt gcagaacggg tgttttttga atttatttta
     1561 ataatttatg aatatatatc agaatgcata aatatattta aatataaaga ttaaaaagaa
     1621 tttaaaggag tgttgataac ttatgataat tccatcaaga agaatacaag atataacatt
     1681 aacaaattta aaaaatggtg aagtaacttt aattgaattg gatgaaatat acaagaagat
     1741 gggattttta tttgttgtta gtgaaggcaa attgaaaaag ataaaaaagg agaatcatca
     1801 ttagaaatga atgaaataat aaatattata atagtttata aaacctaact tatgtagaat
     1861 ataaatactt aagttaggtt tttttgtgat aaagtttata agtactcaat tttataagat
     1921 aaataatggt cttaagtgaa aagtaaataa gtttaaattt tagtaagtat tatattgcat
     1981 aagaggtgat attattatga aaatagtaac ctacaatatt cataaaggta tggattcaaa
     2041 taatagatta actctaacta aaatggggtt atatttaaaa caattggatt gtgatgtaat
     2101 ttgtttgcaa gaagtattat atcctcaatt tttagcttta aaagctgttt taaatatgga
     2161 tggtgtcttt gcgacaaatg taaaaaaagt aaatatgata tatggtattt gtacatttac
     2221 aacatctaaa atgttaaaca gtaatcattt tttcttaact agcaaaaaag aacaacgggg
     2281 agctctttgt ataactatag atgcatatgg aagaattata aatgtaataa atactcactt
     2341 gggacttgat aggcaggaga gagcaaaaca attagacgaa ataatagact ataggaacag
     2401 attagtgggg atagttattt tatgtggtga ttttaatgag aaaaatgtct ttttaagtat
     2461 gtttaatgat atggctattt cactaaacaa gtcttactta ccaacatttg aaaaatcaaa
     2521 ctctagaata gattacattt ttgttaataa aaatactgaa ctaaaaggat acacagtaga
     2581 aaaaatttac ctgtcagacc attatcctgt aataggttac atttaaattt caatataaat
     2641 gaaattagtt gttataaaat aaacaatttt ttgttaagaa atagtaaaag aaatataata
     2701 aaaaatatca aaaataaaac taattataag acataaattt acaataaagt tacatttgat
     2761 taaattcctt gtttaacaaa agctattcaa atataatcta aggtagtttt gaaatgggaa
     2821 ctctattatg caaatttaag ctagtgttaa aatgaggtga agttatgggc agaagaacaa
     2881 caagagtttc aagattaggt aaagaaaaaa aacgaagtgt tttaattgta ttggcagctt
     2941 tagttctaat aatgggtgga tttacttatt tttttaacag taaatttctg tacaatggga
     3001 aaatcgctaa aaatgtatat atagaaggag taaatgtatc tgatatgact aaagcagagg
     3061 cattaaaagc tataacagat aagtatactc cagaagattt aaatcttgct tatgatggga
     3121 aaaaatatac aatatcacca aaagatattg atttaaaata tgatacagaa gatgttgtaa
     3181 aagatgctta tgaaagtaca aaaaaagggt cctattttca aaacttaaaa aaatatattg
     3241 atataagagt gaataaggca aatatgaaga taaaagctga atatgatgag gcaaaactta
     3301 gttccaaagt gtcttctata gcagatagta taaatgttaa gatgaaaaat gcttctatta
     3361 gtgttggaag tggtggacta agttatacag actctgttgt aggtagagaa tttgatttgg
     3421 cagcaaataa agagtctata tataatatga taaaaaataa agagcataaa actttagaac
     3481 ttaaagtaaa tcttcaaaaa ccagatataa caactgaaca agtaaaaact gtaaattctg
     3541 ttataggtca atattcaact acttattcac aagctgtaga gggtaggtca tataatgtag
     3601 gattatcagc aagaaaaact agtgatgtat tattaatgcc aggtgaagaa ttttcataca
     3661 ataaacttac aggacctagt aataaagcaa atggttacaa agatgcacct gttatagttt
     3721 atggtaagct agaacaatca gcaggaggag gtgtttgtca aacatcttca acagtttata
     3781 atgcggctct tctttcgggt atggagataa ctcaagttac aaatcattca tctgcatcta
     3841 cttatgtgcc taaagggaga gatgctactg tgagcgatgg aggattaaac ttaaagttta
     3901 aaaatccata taagcatcct gtatatatta aaaattatgc aggtggagga agtgtatcat
     3961 cagtaatcta tggtaattct ggtgacaaac caaatatatc tatagaggta aaacaaactg
     4021 gtcagaataa atattctaca tatcgaatat ttaaagattc aagtggaaag gtcataaaaa
     4081 aagaacatat atcaaatagt tcttataaag aattaaaaaa atagataatt ttttagagaa
     4141 atatttgtaa tataaaaggg agtgtctcaa aatgaacttt ttagtttatg agacactctt
     4201 tttctgtgaa atcaaatata ttttcatcat ttcaacaata caaaagaagc gtatctctat
     4261 tttgagacag ccttttttgt aaaaattaac actattagtt cttgttttaa tattacaatc
     4321 attaatatat agaaaatatt cctatatatt ggtataattg taaatagatt tagttatata
     4381 aaatgtatga ggaggtgaca tggttgaatt ttataggcaa tagatatgag gtagtaaatt
     4441 ctgacgacgt tttagaaatt aacaaaatct ataaagctag agatgttttt tacaggaaaa
     4501 atgtgttaat aaaagttata aagcacaata attatatctg tgaagatttt gtttcaaatt
     4561 taattgatga aagtactgct ttagatgaga taaactctcc ttatatacta aaaataatag
     4621 atgttgggat tcactgtaca gaagaaacaa cattatatta tgttgtaagt gaagacttta
     4681 atggtatcgg actagatgaa ttaattctag gtaattatct tcatttagaa gcaattgtaa
     4741 acataatgat acaagtttta aaagctttag aaatgataca taggaatcat tcatatcatg
     4801 gtagtttaaa atcgagtagt ataatagtag atacagaata caatataaaa atttgtgatt
     4861 ttggaattac aaaagcaaat aatggggtta atacaagaag ctatggaaac aggagatatt
     4921 tatgcccaca tcaactatgt ataaattata ctgataaaga aagtgatttt tttgcaacag
     4981 gtcttattct atttgaatct atatttaaaa aattgccttt tggagaatca aattcagaag
     5041 aaaaaatgct acaatctatt gataagggat tagattggaa tagtataaaa gcaattaatg
     5101 gaaatactga acttataaat gttataaaaa ggcttcttgg aagaaataat aagtatacta
     5161 gagccaatga tattattatt gatttaagta aagtaatgta tgaaaaagca tatatagaag
     5221 ttgaagatga gaaagaagaa gagcagaaac aagacaaagt tatacaagtt aaaaaagaat
     5281 ataaaagtaa aatgagaaaa aaattacata aaaaacttgt tgttgccggt ctagctatag
     5341 tcatgatttc aatgattatt attagttcca taatataaaa attgtatatt aagtatttta
     5401 aaatcaatgt ctataaagga tatacataga aattttagtg tagtcttaaa ttatgaaagg
     5461 aagtggttat tatgttaaca aaaaataatg aggtattaaa cgtctaggag tattttaaca
     5521 aatagccacc tgcttttaca gtttagaata aaaaagctgt ggctaaaggt cacagctttt
     5581 tgctactttt taaaggcagg agaatattaa aaaggagcaa attatgagca atataaaaat
     5641 aatagcaagt aataaatgtt ggattgaaga tatagcaagg aaacaacttg aagatatttc
     5701 aaagttaaat ggaattttaa gagtagtagg attacctgac ttacatgcag gtaaaatacc
     5761 tgtaggttta gcagttgaga ctaaaaatat tatttatcca catataattg gaaatgatat
     5821 aggatgcgga atgactttat ttaaaactgg ggttctaaaa aagaaattta aaaaagacaa
     5881 atggattaag tcacttagta agattaaaga tttgtctgat atagaaataa aaaacacata
     5941 taaagaagaa tgtccaattt taaatttagg aactataggg agtggaaatc attttgcaga
     6001 aattcaatgt ataagtgaaa tttataataa agaacaattt gaacaattaa aattttcatc
     6061 tgatgatatt atgatgttag ttcactgtgg gtcaagaaat tatggagaag atattttaaa
     6121 aaaattttat gataaagatg gacttgaagt tgaaagtgag aaagctatag attatattaa
     6181 aaaacatgat aatgctttaa tgtgggcaga gcgtaatcgt gaagctatca gtaaaaaaat
     6241 tatgcagtca ataggtacat ctggagatgt tgaaacaata ttctctataa atcataattt
     6301 tatagagaaa agggaagata aatttataca tagaaaaggt gctgtttcaa gtgaaagagg
     6361 agctgtgatt ataccaggtt caagaggcag tctatcatat atagttatgc ctactgaaaa
     6421 tacagagata tctttatatt ctctatcaca tggagcaggt agaaaatggt ctcgttcagt
     6481 ttgtaaatct cgccttaaaa gtaagtatag taaagatact ataaaacaaa ctaaatttaa
     6541 aagtcagatt atatgtaatg atgtaaattt attatttcaa gaagctccag aggcatataa
     6601 gaatatagag cagattattg aaagtttgat agaatatgaa cttattcaag tagtggcaac
     6661 tatgaagcca ttgattactt ataaaggata atttttaaag ttaaaaaaga ttaaaattat
     6721 attataaata gctaatttta tggaacattt aaaagtgttt tattattagc tattttttta
     6781 ttttgtagag acgataattt atagagctaa attctttgat atataatgat tacaaagtta
     6841 gactgtattg gaaactgcac atacatgaat tgatgacaat ataagtgatt cccaaatata
     6901 aatgaaagta taagcttatt tttactataa aaattttcac tgtacaaaaa cattaatgac
     6961 attattttta ttgacataaa tatataattt atggaataat attagaagca ctgatataag
     7021 tgtttaaata agaatagaaa aggagaagtg ttttatgaaa aaaaatacta acattaatga
     7081 gtccggagtt aaacttctta ataaataagg agtttgatat ataataactc cttatttatt
     7141 aagttgttcg caaaataact attatcaaag taaatgatag ggtgatttaa aagacgatga
     7201 cttataaata aaggagaaaa aataatgaat agtaatacat ttgaaagatt acaactaaat
     7261 gaagttaaag acttaataaa aatacattgt gtaagttcgc taggaaaaaa tttaatagat
     7321 aaattaactc ctagtgggaa tattggagta gtaagaagga aattagcaga aaataaagaa
     7381 gctagaaaga taattgaaaa tagtaatcac ataccacttg aaggattgtt taatgtaggt
     7441 tctacaatag ataaaattga aaaaggaatg ataatagaac ctgttgaact tgtaaatatt
     7501 gaagattttt taagaggttg taggaagatg aaagctttta tgttagaaaa agaattttat
     7561 tcacctacat taagttctta tgccttaaat ataacagaat gtaaaagtat agaagatgag
     7621 ataaattact gtataaaatc taataaagtt gattctaatg ctagtaaaga attaaaaaag
     7681 ataagacgaa atatagaaat tacagaagga aagataaaag atagattaaa taaatttata
     7741 acatcaactg taaataaaaa gtatatacaa gagtttataa taagtaaaag aaatgataga
     7801 tatgtaatac caattaaatc ttcttataaa aatgaagtta atggtactat attagataca
     7861 tcatcaaaag gaaatactgt atttattgaa cctattagtg tttcaaattt aagtacagag
     7921 ttaactatgc taaaagctga tgaaactatt gaagaataca aaatattatc ttatttaaca
     7981 gaacttatat ttgagaagat aagtcagata aaattaaata tagagatact ttcagaatac
     8041 gatatggtat ttgcaaaagc taagtatagt caaaaaataa aaggaattac tccaaaaata
     8101 aataataatg gctatataaa aataattaaa ggtaaacatc cacttctaac aggagatgca
     8161 gtacctttag attttgaaat aggcaaaaat tatagaagtc ttataataac aggtcctaat
     8221 gcaggaggaa aaactgtaac ccttaagact gttggtttac taactttaat ggttcaatgt
     8281 ggatttgata tttctgcaaa agaaggtagt gaatttagtg tatttgaaaa agtttttgta
     8341 gatatagggg ataatcaaag tattgaaaat gccttaagta ctttttcatc acatataaaa
     8401 aatattgcag agattatgag tttatcaaat aattctactc ttgttctgtt tgatgaaata
     8461 ggaagtggaa ctgaacctaa tgaaggagct ggtttagcaa tatcattatt agaagagttt
     8521 tacaaaatgg gatgtataac tatagcatca actcattatg gagagataaa aaagtttgct
     8581 acattgcatc cagaatttga aaatgctggt atgatgtttg ataaagaaac gttagaacca
     8641 ttgtataagt taactattgg aaaatctgaa gatagtaatg ctctttttat ttctaaaaaa
     8701 atgggaataa aaaatagagt tttagataga gctaaagaat atgtaattga tagaaactac
     8761 aatttagatt tagttaaaag aagtaagcta caatctaata ttgaagataa aaatagttct
     8821 gaagtcattt taaactatac agattataat gtaggggata aggtaaaatt actagatgaa
     8881 gatgattttg gaattgtata taaacctatg gacaagttta ataatgtaga agtattgttt
     8941 aatgataatt ttatttcagt aaatataaaa agattacaac ttagcataaa agcatcagaa
     9001 ttgtatccag aagggtatga tttagatgta ttatttacta gttttaaaga aagaaaatta
     9061 gaaaaagata ttcaaagagg ttcaaaaaaa gctcttaaaa agattcagaa agaaattaga
     9121 gagaatagaa aataaatata aaaagcacaa aagaaagcaa tctaaagaga aaagtttatc
     9181 tttagattgc tttcttatta acatatcttc ataaatccaa tgactaacaa gattaaacca
     9241 cttaaccaag ataagtttaa atttacgttt ttaacaaaca tatttcctag taaacaacca
     9301 agtaatactg caattatatt tgatattaaa gaaaatagaa taacttgtat ataattcaca
     9361 cctacaaggc cactaccaaa accgatagct aatccatcta aacttaaagc aagtgcaagt
     9421 gtaaatgctt cagcagattt aagcacttta gatttacttg tatctaagac atcaggttcg
     9481 atacttacat taaaatttaa tttaaggtct aacatattaa aattataatt ctttgagtgt
     9541 atagattttt tcttaaaata agatttaaaa aaactttcaa ttagtctagc acttcctaat
     9601 aaaaataaaa taaagaaaca tattaacatt gttatatttt gaggtaaaaa gtctctaact
     9661 attgtacctg caaaaagcga tattgctaaa atagctgaac aaactacgct gataattata
     9721 tttgaatata agggaacttt aactttattt acgccatatc caaagcttgt cacaaaggca
     9781 tctatacaaa gagaagtaac taataaaatt gattcaatca cagtagatta acatcctttc
     9841 tcttaaattt tgtacttata ttagttatag tattgtaaaa atggataaat tgttacagat
     9901 aatccaataa attgttgata aaattccaac atcatacaac acctaaataa caaaagaatc
     9961 atatcatata ataatataaa accaatcata ataaagcggg tgaaaataat ggaacaaaat
    10021 aataaggttg ggaatttcgg tgatttgttg aagattttat tagatgaaaa agaattatca
    10081 atgggagaac taagtaaaaa aagtggaatt gataaatcta ctatatctcg tatagctaat
    10141 aataaacaaa agccaaatat aaatcattta gaaaagatgg caatacattt aaatattaac
    10201 ctagaagaac tgttgaaagc ttcaggatat gaatttagaa acagcaataa tcaaatattt
    10261 aatgcagata gtgattttag taactttgat gatattcttg gatttgcaaa tttaatcaat
    10321 gataagaact ttaatggaaa tattgaaaaa gagttaagta agtgtaagtt atatgtccaa
    10381 acagatgaag gtaaaaaatt actttttgat aattttaata aaaaaataga ttctattgaa
    10441 aaacaaggtc aatttacaga tagattaaga caaatgtatg ctgatttttg tacagatgga
    10501 cttacaataa agaaatattt attgattgga agtatgctac tttattttgt tatatcaaca
    10561 gatgtgattc cagacttcgt attcccaata ggatttatgg atgatttagt agcattaaat
    10621 atagttacaa aattacttaa aaatgataag taagttttag aatcatattt aatatataaa
    10681 taaatcaaag tatataaaat ttattatttt ttatatgaaa caaattaaaa gcacaatgaa
    10741 gtagattaaa aatttaaaac taagaatcta atatatattt tgtaactatt taagtatata
    10801 aaataaaata atatccaaac taatacaaaa tagtaataat tgttttgtac tggaggatat
    10861 atatttatga attgtaaatg gatatctaaa atagatgaga gaaaaaaatg tcatagagaa
    10921 gcagattctt ctggatattg tatctttcat aaagaaaata agtctgatga agaaatacag
    10981 ttgatgatgg atactattca taaagaagag ataagtgaat ttaatggatt tgtttttgaa
    11041 aatgaattta atgcggaaga aatattaact tacaattata aaatactgga tttttctgaa
    11101 tccatattta aacaaaaagc aaattttaaa aaatatattt ttaaaaagaa tattattttt
    11161 aactatacag aatttagaga taaagtttta tttaatggtt gtgtattctt agaaaactgt
    11221 gattttaata gaagcatatt tagtaaaaac tatataaatg accgtatatt tgaaaaagtg
    11281 aaatttaaag gacctgattt ggtagttaat aaagtagaaa attttccaag aatggatgga
    11341 ataattttta gcatgtgtac aaaatttgtg ttaaagaatg ttgaatatgg aaaaagtgag
    11401 tatgagcatg gcaagataaa ttatagaata gctagaaatc aagcaacaaa aattggagaa
    11461 tatgagatga taggatttta ctattataag gaaagaattt atagtagtaa aataatgaaa
    11521 cgttctaatt atcctacttt cagtgattat ttagttgaaa aattttttga ccaaatagca
    11581 cgttacacaa caggttatgg agaaaagcca tggaatatat tattagttat tatagcaata
    11641 ataagtgttt ttgctttatt atacttattt gtaggcatag aaagttcaaa tagcacactt
    11701 gtagctttag atataaataa tataggtgat tactcactaa gtgagatttt caaaatgtat
    11761 atggatttat ggtattttag catggctaca tttagtacag taggatatgg agatatggtt
    11821 gctacaagtt tgattgggaa agcattggca ggtatagaag tattttttgg agttacaata
    11881 ggagcaattt gggcatctgt gattattaaa agaatgataa ggtaatattt gtgtaaatat
    11941 ggataggtat taattactag atataaattg gactaaatag ggaaaattta aatataaata
    12001 tacttaataa tccaagaagg tgaaaaattt gaaaaatgca ctaaaaaaaa ttatatcatt
    12061 agccatgatt ctagttttta taataccaaa ttattcatat gcagatgata aatctgtaga
    12121 caagtattca aaaacatcta tacttataga ccaagaaaca ggtagagttt tatatagtaa
    12181 agaacctgat atgaaggtac cattagctag tttaagtaag atgatgactt ttttacttgc
    12241 aatagaagct attgaaaata aagaagtaaa agaaactgat aaagtaaaaa tagacaaatc
    12301 tattgcatca gtaaaagggt caagttataa acttaaagat ggtgaagaag tgccacttat
    12361 agagcttatg agaggtctta tgattgtatc aggaaatgat gcggcaattg ctatagcaaa
    12421 acatatttgt aaaacgaaag aagcatttgt agatagaatg aataaaaaag caaaagaaat
    12481 aggaatgagt aatacacatt ttctaaatcc aaatggtctt ccaatatacg atttaagcaa
    12541 ccccaaaaaa ccagcaaaag aaaatatctc tactgcaaaa gatatagcta ttcttggaaa
    12601 atacatgttt gaccattatg aaaaacaagt aacagctata actgatatgg agacatatac
    12661 aaattcagat agaagttttg aaaaaagcaa tacaaatgca ttattaagaa ttataccaga
    12721 agtagatggt ataaaaactg gatatactgg caatgctgga tattgcttat cattttctat
    12781 gattgtaaat aaaaatgata aaaatgagaa aaaacgtaga gtaatagggg ttgtattagg
    12841 tactaaccat aaaaataaaa gaacctctgc ttcattagct ctactgaaat atggaaaaga
    12901 aaatttcaat atgaaaaaag taattgataa agatagcttt attggtaaga aatatataaa
    12961 aggaatgaaa gagttagagg ttacattaaa agcaaaagat gaactttata caatattaaa
    13021 agatgatgaa agtataaaat cagaagttaa gattaaaaat atagagtacc ctgttaaaaa
    13081 aggtgatact atgggtatta taaaatatta tactaattca ggtgacttac ttggaagtgt
    13141 agatatagta agtaataata gtatagataa cgtatcatta aaaacaaaat taaaaatgaa
    13201 atttgcaaat taaattcaaa aaatggtata aaatacacaa aaaaatataa aaatagaaaa
    13261 tatggaaaaa atattgttat ttcttcacct ctgtgatata ctactagagg tgaaaaaata
    13321 aaataaatca cacacagcat tgggatcctt aaaaggtgcc tattacatgg ttttttaggg
    13381 agaagatcaa gctggaggta taaaaccagg aggtaagata tgtcagtaat atcaatgaaa
    13441 caattattag aagcaggtgt tcactttggt caccaaacaa gaagatggaa ccctaaaatg
    13501 gctaagtata tattcacaga gagaaatgga atatatataa tcgacttaca aaaaacagtt
    13561 aaaaaagttg aagaagctta caagttcact aaagaagtag ctgaaacagg aaagccaatc
    13621 ttattcgtag gaactaaaaa acaagctcaa gatgcgataa aagatgaagc tgaaagatgt
    13681 ggaatgtact ttgttaatga aagatggtta ggtggaatgt taacaaacca caaaactata
    13741 aaaacaagaa taaataaatt aagagaatta gaaaaaatgg aagaagaagg cgtatttaat
    13801 gttcttccta aaaaagaagt tataaaatta agagctgaaa aagaaaaact agaaaaatac
    13861 ttaggtggaa taaaagacat gcctgaatta ccaggtgcaa tgttcgtagt tgacccaaga
    13921 aaagaaaaca tagctattca agaagctcat agattaggta taccagtagt tggtatagtt
    13981 gatactaact gtgacccaga acaattagac tttgctatac caggaaatga tgacgctata
    14041 agagccgtaa aattaataac tggtgctatg gcaacagctg taattgaagg tagacaaggt
    14101 gctgaggaag aagtagctga agaccaagaa taataatttg acaattaaat ggtaagggtc
    14161 gcccttacca tttataatat ggaatagaat aagtaggagg aaacttaaaa aatggctaat
    14221 ataactgctc aaatggtaaa agagttaaga gaaagtacag gcgcaggaat gatggactgc
    14281 aagaaagctt tacaagaagc tgaaggaaac atggaaaaag cagtagattt attaagagaa
    14341 aaaggattat caaaagctgc taaaaaagct ggtagagttg ctgctgaagg tctagttgca
    14401 atagaaatga atgatgataa tacagttgct tctatggtag aagttaactc agagacagat
    14461 ttcgttgcta aaaacgaaga cttcaaagtg ttcgttaaag atgctgcttg tatggcatta
    14521 gctactgata aagaagatat agcttcttta ttaggtgaaa ctcataaaga gggaataact
    14581 ttacaagaag ttttaaataa tagagttgca aaaataggtg agaaattaga ctttagaaga
    14641 tttgctaaag ttgtaactaa tggacaagtt gctggataca ttcacggtgg tggaaaaata
    14701 ggtgttcttg ttgaaatgga aacagaagct agagatgcta aagttttaga attaggtaaa
    14761 gatgtagcta tgcaagttgc tgctatgaat cctaagtatg tttcaagaga tgaagtagat
    14821 gctgaataca tagcacatga aacagaagta ttaactcaac aagctttaaa tgaaggaaaa
    14881 ccagctaata tagttgaaaa aatggttaaa ggaagattag aaaaagaatt aaaagaggtt
    14941 tgtttattag agcaaacttt cgttaaaaat ccagatataa ctgttaaaca attagttgct
    15001 gatgttgcta aggcagtagg ttctgatata aaagttgtta aagttgtgag attcgaagtt
    15061 ggtgaaggta tacaaaagag agaagaaaac tttgcagaag aagttgctaa gcaacttaag
    15121 taataaccaa agagagaaca cgcaggtgtt ctctttttta aaaaatagaa aaaattggat
    15181 atgaaggatt gaaataggag gtttctgatg gataaaccaa tgtacaaaag ggtattgtta
    15241 aaattaagtg gagaagcatt agcaggagaa agaggttttg gtataaataa tgatgtagtt
    15301 aatgatattg ctattgctat aaaaaaaata caagaaatag gtgttgaagt agctgtagta
    15361 gtaggtggag gaaacttctg gagaggcaga accagtgaag gaatggacag aactactgct
    15421 gactatatag gaatgttagc tacagttatg aatgcaatgg cgctacagga tgcactagaa
    15481 aatatagatg ttgctacaag agttcaaact gctatagaca tgagacaaat tgcagaacca
    15541 tatataagaa gaagagcagt tagacactta gaaaaggaaa gagttgttat atttggtgca
    15601 ggaacaggaa acccttactt tacaacagat acaactgctg ctttacgtgc tgctgagatg
    15661 gaagcagaag ttatattgtt agctaaaaat gttgatgcag tatatgacaa ggatccaaaa
    15721 gtacatgcag atgctaagaa atttacagaa ttaagttaca tggaagttat acagaaagaa
    15781 ttaaaagtaa tggattctac agctacatct ttatgtatgg ataataaaat tccaataaag
    15841 gtatttgagc ttacaacaga gaatataata agagcagtaa aaggcgaaaa tataggaact
    15901 actgtaaaat aatgaagaat gaaggaggag taaaattatg aaactggaaa tacataagca
    15961 attagaagag aaaatgaatg ggacaattga tgctttaaag tttgaatttg gaactataag
    16021 agcaggaaga gctaatgcac aaatgctaga caaaataaga gttgattatt atggaactcc
    16081 aactccaata aatcaaatag gtgctatatc tgtaccagaa ccaagaatat taatgatatc
    16141 tccttgggat aaatctgcta tgcatgaaat agaaaaagcg atagctaact cagacttagg
    16201 attaaatcca tcaaatgatg gtgaggttat aagactttct gtacctgctt taacagaaga
    16261 aagaagaaaa gaattagcta aaaaagctag taaagctgca gaagaattta aagttagaat
    16321 aagaaatgaa agaagagacg ccaatgagaa aattaaaaag atggaaaaag gcggagaatt
    16381 aactgaagat gaattaaaaa aagcacaaga tgaagttcaa aaaatgactg ataaatttat
    16441 aaaagaaata gatgcattat tatctaaaaa agaaaaggac ataatggagg tataatattg
    16501 aaaaaatatt ataacctttt aggtcttcat gtagatgagg taaaacaata ttttgatgag
    16561 caaaatataa attataccgt aaaatctatt gaaggtaaaa aggataaaga aaaacttgta
    16621 gttcctagag ttataaaaat atctgagata ggtgatagtg tagaactgat aatcacatac
    16681 ttttctgact ccttagttta aggagtcaga acaatattgg aggcaaaata tgaataacaa
    16741 cattatatat gatatagact taaataacat acctactcat attgctatta taatggatgg
    16801 aaatggaaga tgggcaaaag ctagatttct tccaagaact gcaggacata aggctggagt
    16861 agaaactata agagatatag taaaagaatg ctcaaaatta ggtgttaagc acttaacatt
    16921 atatgcattt tcaacagaaa actggaaaag accgaaactt gaagttgaca cacttatgaa
    16981 cttattatct acatacttaa gaaatgaaat agctgaactt caccaaaaca atgtaaaagt
    17041 aactgctata ggagatataa gtgctttacc aaaaacttgt ataagagaat taaactcagc
    17101 taaggaactg acaaaaaaca atacaggagt taacttaaat ttagcattaa actacggaag
    17161 tagagcagat ataaaaaatg ctcttataga tatagtaaaa aattgtgaaa gtggtaaaat
    17221 agatataaac aatattgatg aagatattat aaaaaactat ttaagcacaa agtctatacc
    17281 agatccagac ttggtaataa gaacaagtgg tgaacaaaga ttgagtaact ttttactttg
    17341 ggaagttgct tattctgagt tttactttac tgatatacat tggcctgatt ttaaaaaaga
    17401 agaactacag aaggctatat acgtatatca aagaagggat agaagatttg gaggacttaa
    17461 gtaaggagat tattttatgc ttacaagaat tattgcttca ctagctctgg ttccattatt
    17521 tctatttgtt gtatatggag gtattcctct atatatagct gagacagcaa tagtttatat
    17581 tgcattgcat gagttttata aggcatttaa aataaaagat gtacatccaa tattcattat
    17641 aggatattta ttttctattt atttagctgt aaaaaatatt tttaatttac cattagaata
    17701 tacttatgca gtaattttta ttttattttt agcaagtata atttatatgt tgatgggcaa
    17761 aaacaatgtt atagatgttt cgattacgtt tttaggagtt ttttatatag gagtattttt
    17821 agattttata attataacta taaatggatt tgaaaaaggt agtatatatg tatggcttat
    17881 atttgtgata tcttttatga cagatatatt tgcatatttt agtggttatt tacttggaaa
    17941 acataagcta attcctaaag ttagtcctaa aaagactata gaaggagcta ttggaggaat
    18001 aataggaagt acactttgtt gtattttatt tggatattta tttggcattg atttgcttca
    18061 attagcgata ataggaagta taggaagtgt aatagctcaa ttaggagatt tatttgcatc
    18121 ttctataaag agatatgtag ggataaagga ttatggaaag ataatacctg gtcatggggg
    18181 tatattagat agatttgata gtgttatatt agttgcacct tttgtatata gtgcgattaa
    18241 attttttatt agataaagat tggttggatt taaaagagca atctataatt gctagtaatg
    18301 aaaatagcta tgtaactttt aaatgcagag atattctgca gttattagtt acatggctat
    18361 tttcattgtt caaaaattta gaagaccttg gggaattgta taagaatagg tattacttta
    18421 taatagtata ctatatttgt agtgagtata ttttttatac tgtaactatc attaaaaaat
    18481 atttttttgt ttaataattt cgatattttt agctttatta tatatttata atatat
//
LOCUS       DAMJZE010000074        18523 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJZE010000074 DAMJZE010000000
VERSION     DAMJZE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.074"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..591)
                     /locus_tag="PC541_002116"
     CDS             complement(<1..591)
                     /locus_tag="PC541_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2839582.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC541_002117"
     CDS             complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC541_002117"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2839583.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2758..3297)
                     /locus_tag="PC541_002118"
                     /pseudo
     CDS             complement(2758..3297)
                     /locus_tag="PC541_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(3374..5179)
                     /locus_tag="PC541_002119"
     CDS             complement(3374..5179)
                     /locus_tag="PC541_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839584.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5575..5952)
                     /locus_tag="PC541_002120"
     CDS             complement(5575..5952)
                     /locus_tag="PC541_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2839585.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6857..7342
                     /locus_tag="PC541_002121"
     CDS             6857..7342
                     /locus_tag="PC541_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839586.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7542..8081)
                     /locus_tag="PC541_002122"
     CDS             complement(7542..8081)
                     /locus_tag="PC541_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2839587.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8074..8487)
                     /locus_tag="PC541_002123"
     CDS             complement(8074..8487)
                     /locus_tag="PC541_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2839588.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8523..9644)
                     /locus_tag="PC541_002124"
     CDS             complement(8523..9644)
                     /locus_tag="PC541_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2839589.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10232..11884
                     /locus_tag="PC541_002125"
     CDS             10232..11884
                     /locus_tag="PC541_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2839590.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12183..12932
                     /locus_tag="PC541_002126"
     CDS             12183..12932
                     /locus_tag="PC541_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839591.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12945..14972
                     /locus_tag="PC541_002127"
     CDS             12945..14972
                     /locus_tag="PC541_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839592.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            15116..16699
                     /locus_tag="PC541_002128"
     CDS             15116..16699
                     /locus_tag="PC541_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2839593.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16671..17291
                     /locus_tag="PC541_002129"
     CDS             16671..17291
                     /locus_tag="PC541_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839594.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17480..18406)
                     /locus_tag="PC541_002130"
     CDS             complement(17480..18406)
                     /locus_tag="PC541_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2839595.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         6348 a         2915 c         2227 g         7033 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcatttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt ttattttgaa tttttcattt gctttacttt
      781 tttatttgct ttactttttc atttatttag atttttcatt tgttttagtt tttattttat
      841 ttaaagtttt cacttattta agtttttatt ttccttaaat ttttcattta tttgttaaga
      901 ttttcttttc cttaagtctt ttatttactt tggcattcac cattccaaac tatatctcta
      961 tatttatcag ggtcagtatc tccttctgta cttattaata atactttaga gttctcatct
     1021 agtttcaaag cttctcttaa gtcttttaaa tcttcacttt caaggattga aactaatgct
     1081 ccagttgtaa ctgctccaga ttctccagag attactttct catctcctct tagagggttt
     1141 cctaatattc tcataccttt agcagatacc cagtctggtg ctgaaacgaa tgctgatgaa
     1201 taatttttta acacttcaaa acctatagtg tttggttctc cacaagcaag ccctgccatt
     1261 atagttggca tatctcctcc aacaaatctt ggttttccat ctccagcttc tgctgattta
     1321 tagtaacagt cagcctcatc agcctctact actactgtta ttgggcattc atcatatatt
     1381 gaagcaacat atccttgaac tgcaccagca agtgaaccaa ctcctgcttg aacaaatacg
     1441 tgtgttggtc tatctactcc atattctttt aattgttcta tagcttcttt agccatagtt
     1501 ccatatcctt gcattatcca tgctggtatt tcttcatatc cgtcccatgc tgtatcttga
     1561 accattactc catggtcatc agcttctgca taatcattag ctaatcttac tgcatcatca
     1621 tagtttaagt ctgttatact agcatctgca ccttctcctt tgattttatt taatctaaat
     1681 tcagaagaac cttttggcat taatacaaca gatttttgac ctaatttatt agctgtccaa
     1741 gcaactcctc taccatgatt tccatctgta gctgtaaaga aagttatttc tcctaatttt
     1801 tcttttattt cttttgaagt taatttatca taccctaact cagatatatc tgtatctaat
     1861 ctttgagcta agtatctacc catagagtaa gaaccaccta aaactttaaa tgcgtttaaa
     1921 ccaaatctat aagattcgtc tttaacatat attccgctta cgcctagtga ttttgctaag
     1981 ttatctaatt taactaaagg agttttttca tattgtggaa agctttcatg aaaagcttta
     2041 gcctttgcaa tctcttcatc ttttaaaaat tcaattcctt tttctttttc ctctgattta
     2101 ggtaagttat ttaatttcca ttttatttct ctcatttatt ttatattccc ctttcaaatt
     2161 tttcggtgtc attttaattt tttatctttg tgccttatag caaagcaatt accgtgccaa
     2221 tattatttat tctaaaaaca atttttttat gttggaatta catactttta taaaatttct
     2281 ttaaagttta taaattttga gtgtgaaaaa taagatttat aaaagaaaaa attctcatat
     2341 tgggaaattt ttatcaatac gagaattatc aattcgagaa atcgtttatt tatcttaatt
     2401 cttcctttat tatgctctta ttttttatat gtctattgtt ttgtcagata tattactact
     2461 tttattttct ataaatatat ggctataacc ttcttacgca taaactctat aaatttatat
     2521 actttatatt ctttattacc caaatattct ttattaccca aagcctccaa aatggcttaa
     2581 atcttttcat agcttcttac agaagtttac atgggcgtcc ccccaataag caaaagtgca
     2641 atatgtgtac cattgtgccc tacttgcgac tattgagctg tcgaaattcg caacttcgca
     2701 tttatctgat gaaatgcact atacaaaata ttcataacat ccacaacaga gttaaagtta
     2761 cctattcata ttcactgtaa aaatttcttc cacaggaaat gtaattttaa ccaatcctat
     2821 tgagacaaac tcaccactta tttttttgaa ctcacgcagt aatacatcac ttgcccgaaa
     2881 aaaagcctca ctttcatcat ctccagtaag tgctattgtg caatggggta cccaacgatt
     2941 ggggtggtac cattcccaac catttgaatc aaaatcttgt agtacctcat ataattctct
     3001 ctgaaactgc tacatactgc tattcattat aggtgataca aacaccgttt tagtatcatt
     3061 aaacatgcct actgaaccaa tgtaagcagg tattacctta tggttttgtg caaacccctt
     3121 tagcttgtct atacaacaag cctcatccac atcattgaaa caagccaaag taagatgagg
     3181 tcttgttttc cattctaaat acttcgtact aattttttca tcagcgattt ttttagataa
     3241 atcaaaaagt tgcttttccg tttttttatc ataatacagc tctatcgcat attgcataat
     3301 aacacctcca ccataaagta aaaataccta tctataaaaa cacacaaata cctatcattt
     3361 taatattata ttactactgt tcctcttcca attttctata tagtgtagcc aaacctatgc
     3421 ctaagtcttt tgctatcttc ttcttcgtct ttgtatctct tccatactta ttcaacaact
     3481 tgtttatgta agtcaactct aattctttaa ttgaaagtat cctttcttga ttctctacta
     3541 tccctccaac tatatcatct tcaaatatta tatttacatc cttattttta taaatatttc
     3601 cattaatatt ataatagttt aaaatatcta atggaagcat atctttatat attattccat
     3661 catctccaac caaattcatc atatattcaa ctgcattttc cagttccctt atatttcccg
     3721 gccaattata gttaatcaac atattcatta cattttcttc tattttattt aactgaatac
     3781 ctaattctaa agaatattta gaagcaaact tcatcattat tgcctcaata tcatcctttc
     3841 tttccctaag aggtggcaaa tttataggaa ttacattcaa tctatagtat aaatcatccc
     3901 taaacttacc ttcatttact aatttcaata agtttttatt tgtagcagca atcactctta
     3961 tatctaaatc tataagttta tttgacccaa ttctcgcaaa ttttctctct tgtaaaactc
     4021 taagtaactt tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga aatataacac
     4081 cttcatttgc aagttcaaat ttacctactt tacctccact actagctcca ctaaatgcac
     4141 cttttatata gccaaatagc tcactttcaa ggagattttc tggaatagca gcacagttta
     4201 tcgcaataaa tggcttgttc catctgctac cttcagcatg tatagcccta gcaattagct
     4261 ctttacccgt accactttcc ccagttataa gtacagttga atttgacttt gctaatttct
     4321 tagttctctc ttttactttt aacattgctt ctgaatttcc aattatagac tctatatcac
     4381 tatttcccca gccactattc acttttccat gtcctttatg attgatatat gtcttgtcaa
     4441 atattattat tttgtcgtat tgatttatat atggatatac cggaattatt ttggcattta
     4501 tattataaat tttgttatca atatcaattt ctagtaattc atgtccattt gaggaagatt
     4561 caacagatac tatatttact atattttcta ttatattttt atctatatct aattttttta
     4621 gagctatatt gtttatataa gatattttgc tatttatgtc tagtattata acgccttttt
     4681 caacattttc aatgatttct atcatcatat ccatttttct actgctattt tttgacgctt
     4741 catgttcttc tattttcata gaaattaaat cgcatatatg attgacaaat tttaaataat
     4801 tatctatttt tgcacttatt tctacttttt tagttttatc tgtactaata gcaccaatta
     4861 ctcctatagt tcgattatta tactttattg gaactgctat tattacttta tttaggcatt
     4921 tcatgtaatg ggaacattct atacaaagct gattatcaca tatatccaat acgactaatt
     4981 cctgacctgt ctgtataaca ttgtcataaa taaagccctc agtaacaacg ctttcattta
     5041 tctttgattt atacagacct gtaccactaa ttcttattaa tctatcgtca actatttcaa
     5101 tatctacatc taacacacta gctactgttt cagtgtattt tatcatttct tgttgaaatt
     5161 cttttaattt acttaacata gtttccccca ttagaattta atttaccaaa ttatcagttt
     5221 agctatattt taacatatag tcactcttta aaatatatat aaaacattac ttatttatat
     5281 atttgcgtta aaacagacaa tattaattta taaaaagttt gttttatata gtaaccttaa
     5341 ttataatatg cagacaataa aataaatcac aatacaataa taaaaaaaag ctttcctcat
     5401 ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc aatagttaaa
     5461 tatttagata gatttttata tagacttttt taatataaac ttttttaatt gtagactttt
     5521 tttattagtt tttaattttt attagttttg ttattggttt tattgttata atacttataa
     5581 tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa cttgtacaca
     5641 tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa tatcattaaa
     5701 atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac ttgtaccagc
     5761 ttgttctaat attgctttta cattatctaa agaagctttt gttgcttctt ttgggtcaga
     5821 tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa ctatgtttcc
     5881 aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg tagctattac
     5941 tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa ataaaatttc
     6001 ctaaacacca atattaatac gaacctttag attaaacttc aaatctaaaa cttcaaataa
     6061 tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa gcaattaacg
     6121 tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt acttctgaat
     6181 tttttcgtaa aaaaacgctc taaaattact tttttatgat gtcaattttt ttctcatatc
     6241 tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa gttatttttt
     6301 aaccttaaat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt
     6361 gcataatcaa gttattacat atgaaagaac tatagttgac aatcccacta ttataaataa
     6421 taaaaccttt acccatacct tatttaccct agttatcata gagcctattg aaaatataac
     6481 agctaaatat aataataacc aaaaaattat caatatctac cactgttgct gaaaatccaa
     6541 aaaactacct tttgatagta ttacttaaca atctaattaa tctcataata aatttacaac
     6601 ttataaataa attgttatat tggccgtatc tcaacaactt tctcctttcc tagttttaaa
     6661 tcttttatag aattgtacca cgtctaagta aattttcata ctattaagtc ttatttctaa
     6721 taaatcatta gctctcattc tatgcccaat aatgtaagat tgcagtaatt ttattgattt
     6781 taatagtttt atttaatata cttatattaa ttattttcat atgaaattat tatttatata
     6841 gggggatttt tttaatatga gcaaaagtga attaacagca gaaacaacag aagaaatgtt
     6901 agaagtacta agtggtaaag attatgatat tgcatgtcat ttacatgaac ttggtaaatc
     6961 attagattgt aaaattgaac caaaaacagg tgctcgttct tacaaaatag tatattcaac
     7021 taagaaacca aaacgtagct tatttactat tgaatgtaat gaaaaaaaat ggagagttaa
     7081 agcaaatctt tttcatctaa atacatataa agatgctgtg gaggaatgct ctaaaactat
     7141 taaagatagt ataactaaaa ctcgtacttg taagaaatgt aattcaaagt gtattggagg
     7201 ttcttcgttt gaattagatg gaaagtctta cctgacttgc ataggaagtg gtcattattt
     7261 tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa ttaattacta aagaaaataa
     7321 tattatgcag gaatctgtat agttttttaa gggtaattat taaaatatat gagtaacaac
     7381 agatacttaa ataggcataa aaaaataaag ggtataataa ggatatagaa taaaaataaa
     7441 tgatatagca caaagaggct gtctcaaaat acagataagc ctctttttat tgttgaaatg
     7501 ataagaatat attcaatttt atacaatttt atacaaacat actacgccaa cttacccatt
     7561 tcatatgcct gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca
     7621 ccatatacta tacctttttc cttagaacca tttagacatg atgtaaatcc tctaaatcct
     7681 tctattgttc tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa
     7741 tctttatcac aaatttcagt atatctagag caagttctat caatcatagt tttcatttgc
     7801 gcattcatag tataaaaata aactggagtc gctaaaacaa tcacatctgc ctcaatcatt
     7861 ttttcaagaa tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa
     7921 gcaccacatc cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga
     7981 tttttagatt ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct
     8041 tttctaggac tagctgatac tattaataca tttttactca taagatactg cctccatttt
     8101 cttatttcta ctgctcttta atactttttc tttctcaata ataccttctt catatctatc
     8161 aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg
     8221 gtttctctgc tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa
     8281 catagctaca tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat
     8341 gaaattaacc cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt
     8401 tacaggtggt attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta
     8461 ttttttacta acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg
     8521 aattatttct caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga
     8581 ggacagcttt cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta
     8641 gtataattat tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct
     8701 gcattgtaca acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa
     8761 tactgacagg cagtacaagg aactaaaatt gattcgttga taatctctac tgcttcatct
     8821 ataatattat attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct
     8881 aataattgtt ccatatttga catcccactt aagaccatca taacattatc tttacttgct
     8941 gcaaaacgta ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc
     9001 tctgcttttt ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca
     9061 ggcttattat gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc
     9121 caatctaaat aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact
     9181 ttatccaata attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct
     9241 tttttctttt cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac
     9301 acgcctatat tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc
     9361 tcattaaaaa tggtttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta
     9421 gctatagtaa aactctctct tttgtgtctt tttactagag attctctaag tgctatctca
     9481 ctttttccca tgtgatacac ataggctgta tcaaaatatg taaatcccct ttctaaaaaa
     9541 gtgtctacca ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt
     9601 attggtaaac gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct
     9661 ttaaaacttg tttgtttttt ctctctactt taactattct tacaagtata taattcttgt
     9721 ataattatac tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga
     9781 caaatattat aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc
     9841 aattctcaaa cttcacaaac tccaacatgt aaagaaatat atactttaag aaattcagtc
     9901 cattaatatt actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaagat
     9961 ttagaaatag gtcttgtgtc tattttaaaa tatataaaac agtggtaaag acttatttat
    10021 aagataaaaa atgaatagtg tatacccttt ttaaaaagtt ttttcctttt tatatttgtg
    10081 atacaatgtt aaatataaaa aggaaaattt tgtattaaaa aaatcttatt tgttgatttt
    10141 gtaacttcaa actcagcatc aatttgattt taagattata ttttattaag tatgctaaat
    10201 aacaaatttt taaagattgt gaggtaaaaa tatgaataag aaaaatttag ctatattaat
    10261 atcaggtatt atgttttttt ctagttcttc agcaattttt gcagacaata ctacaaagca
    10321 agaaagatta ataggtaaaa caagatatga aacagcagta gaagtaagta aattgggatg
    10381 ggttcaatca aaaacagcca ttatagttaa tggtaattct attcaaagtg cgctttgtgc
    10441 aaatccattt gctaagctaa agaatgcacc tatactttta gtaaacaaca acagtattga
    10501 aaattcaact aaggctgagc ttaaaaggct tggtgtagat aatgtgtaca ttgtagatag
    10561 tggaaatagc atttcttcta aagtagaaaa tgaaataaaa tcacttaaca taaaaataaa
    10621 taaaatagta ggaaacaata tatatgaaat gtctacaaat gtacttaaag aaatagataa
    10681 gataaaaaag gtagaaaatg ttgcagttgt aaatggacta aagggactat ctgatgcagt
    10741 tagtatagct tctccagcag caatacataa tatgcctata atattagtat cagatgaaaa
    10801 cagcaatgat agtagtataa atttcttaaa caataggtct ataaaaaaat cttacatagt
    10861 tggaggaaat aagattttat cagacaatac agtaaagaaa tatccaaaca gagaaagaat
    10921 cagtggttca gatagaaaca atactaatgc aaaaattatt gataaatttt ataaagataa
    10981 aaaaataaat aatgtatttg tagctaaaga tggtattaac caagaaaatg cattagtaga
    11041 ttcattatca ataggagtac tagctgctaa agaaaattct cctataatga tatctaatgg
    11101 tagccttgta aatagtcaaa aagaatttat taaaaataaa gatatagatg aaataacaca
    11161 agtaggagga ggatataatc aaaaagcatt tgaagatatt ttaaaactaa aaaatgtgaa
    11221 cataagaaat aaaactaaaa aaatatcttt aatacaggct aaagaaatat taaaacaagc
    11281 tcttccagga agcaatattt tagtttgtta ttatgatgaa gatgataatg aatatgactg
    11341 caaagttata aaggataata tcctatatga gatagaaatt gatgcctact ctggtaaaat
    11401 aatagaaatt gaaaaagatg atgacttaaa tgataataat ttaggtacac aaaatatatc
    11461 attggataaa gctaagtcta tagcattaga tgctgttcct aatggtaatt taatctattg
    11521 taaatatgat gctgaagata acgaatatga ggctaaagta ataaaaaata agaaaacata
    11581 tgaattagag ataagtgcat ttgatggaag aattgtagat attgacatgg acaatgattc
    11641 tgatgatgac tttgatgata atgataagaa taactctaat actaatgaca acagttctat
    11701 tattagtgct gaaaaagcta aatcaataat gctagataaa gtgcctggtg ggaaaatagt
    11761 aaaatttaag tatgatgaag atgacaaaga atatgaaggt gaaataataa aaggaaaaac
    11821 tgaatatgag ataacaatta atgcaattac tggtaaaata ttagaatttg aaatagatga
    11881 ttaataaaaa atatatattc tattaattat tacaagcaat aaagccataa atattgaaaa
    11941 tttcaatatt tatggcttta ttttattttt tttaatttaa atccacatta tgccattata
    12001 tatacctatg cactctttta aattaaaatc ttgtccatca tcccaaacct aataaagtta
    12061 gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg tgatttactc
    12121 atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag gagacatata
    12181 caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa gggcaacagc
    12241 cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt ataatgggag
    12301 cttctggctc tgggaaaaca actctactta atgtattatc tacaattgat aaaccaacaa
    12361 gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact tgtgctgctg
    12421 attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta gatagtctta
    12481 caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca gataaaatag
    12541 aaaacatggt aaagagtcta gctgaacgat ttggcatagc ttctcaatta aataaatacc
    12601 caaatgaatt atctggtggg caaagacaga gagcttcagc agttagagca atagtaaaac
    12661 atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat tcagctactg
    12721 aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt ttaatggtaa
    12781 cacatgatgc ctatgtagca agttttgcaa atagaatttt gatttttaaa gatggtcata
    12841 tcattaaaga attaaataaa caacagaata atagacatac attttatgag ttgattattc
    12901 aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac attttttata
    12961 cggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta ctttcagtct
    13021 cttttgttat tactgtattt ttcgcattta cttcattttc tgttaataag ataatgctag
    13081 aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg atatttttga
    13141 tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga cgtcgtacaa
    13201 aagaattagg tatatatgct ttacttggct atcgtaagtt tacaatatta tcgctactta
    13261 cctttgaaaa tgtattaatt tgtagtggag cttttattat tggattggta ttaggagcat
    13321 tggcacataa gggtattgtc tttggaatta cagcactact taatttatca attgataatt
    13381 cacagatacc attttttaat ataaatgcaa taaataaaac aattatcttt atagcattag
    13441 ttgttttagt cttagctgta tcaaatggta aattttttta taagactcca cttgtaaaat
    13501 tgataagata tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt ccagccttta
    13561 tggggtttat tatgatttta agcgggtatt gtttatcact agatattctt cgtggagcta
    13621 aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg attatcattg
    13681 taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa aatagtaaaa
    13741 aaaataaacg tactttctac acagatatga agataatagc tattccaaac tttatctatc
    13801 gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct actactttga
    13861 cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt tcgcgtattg
    13921 caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat gtaaaacaaa
    13981 ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca aatatttata
    14041 aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca tctaagaata
    14101 atttatccaa tagtagttct tctaaagaag ttatttcaag agaatcagga tttgaatgta
    14161 tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag ctactaaaac
    14221 aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca aataatgatg
    14281 gatatagtga aaatggaacg atttatccat tacttgttaa tggtaaagaa acacctgtag
    14341 ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt ggaacactta
    14401 tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg acaagaattt
    14461 taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat atttcagatt
    14521 atttagatgg aagcccttat ttacaaggat attctcacag gataaatgat ttattatcaa
    14581 ttaatagttc tacatttctg ttaatcggat ttcttgtggt attattcttt attgctgttg
    14641 gaagtatttt atactttaac aatatatcag caatttcaga ttcaacatct gattataaaa
    14701 ttttacagaa aatgggttat actaatatac aaattaaaaa aattattaaa aaacaagtat
    14761 tgccattttt taatattccg tttttacttg gtatagtgga ctgtatattt gctaccttgg
    14821 tttataagac tgctttgatg cagaatttac ttggaaattc actttcacaa tacatacctg
    14881 tgattattgc aattttctta acttctgtag tatatttgat atattacata cttactatta
    14941 aatcctgttg taaaattgta ttaaaaaaat aatatttgcc aaatcgtcaa gcttaaaggt
    15001 gtgacgattt ggtatacttc atttataagt atttgatata atttaaaact ataaattctt
    15061 aagtttgatt tagtcataaa attaatataa ttaactcata aggaggtgta taagattgac
    15121 aaattttaat ttaaaaatac tatttttagg aaactacatt ctatcacttc tcttcatggg
    15181 aatatctaca agcactgaaa cttatatgaa tattcaaagc attattccct taataatttc
    15241 aacattatta ctcatagaca tcacatatat aacctcttgt aatgtccgag aaaataacat
    15301 tatttcattg ttttgtggat tactagcact agacagttgg tatttactct tatcatttgt
    15361 aaccacacca ataggcacta ttatttttaa aatattaagc acagttatag tgtatgtctc
    15421 tattaaattt atatttttat ttttgtttca aggtagtgga tacaatttcc gtaagataac
    15481 agacatttta ttgcttatca cctgcattgg tgcaattgta ggagcattta tttctaacag
    15541 aacgtatgct tgtatgtacg gaatacagtt tgttgttagt ataataagct ttttctatat
    15601 tattttttat catttgaaaa gggttgtttt tgtcataaaa agcgaaaaaa aacctatatc
    15661 gatttcagta tttataacaa ttataggatt ccttacttat tattttttta cactaagaat
    15721 atcaaatcat attggtaatt ttggaatcta tctaactgta ctggtatttt ttatgagtat
    15781 tcatggtatt gcacttaaag aaaatgatag ttttcctatt tcaacagttt ttaacataaa
    15841 acaaagggct atatttcttt ttataagtat tttagtaatt agcttggtcg tactatttgc
    15901 caatgggaat ttttctataa tattaatagc tataaatgtt ttatttatgc tcattttcct
    15961 ctgtaacatt attttggagt ttaaccttag agatgaaaaa aataaagtag ttaaaaatag
    16021 caagtatact tttgcattag aaaaattaca acaagaagaa caactaaaaa cagagttttc
    16081 taatttttta catgatgaca tattacagaa cctgctttca ataaaaaaca tgatgagtaa
    16141 gtcatatcgc ccagatgttc aagatattat atatgaaaca cttaacaatc taaatacatg
    16201 tataaggaag caaatgcaag actatcatcc tgttatttta aaaaatttaa cagtaaaaga
    16261 aaattatcag aatttaattg aaagtgtttc ttcatccttt ccacaaaaaa atatttgtgt
    16321 ttcatttaag tgttctgata ctttattctt agttgaacca tatgatatat tgatatatcg
    16381 actgattaaa gaattattga caaatgtgta taaacattca aatggtagcc atgcatggat
    16441 agctttagca ctagaaaaaa atataataaa attaaatatt agtgataatg gtgtggcaaa
    16501 tacatctttt aaaacgcctt tagatataac taaaattact tttgatacaa taaaacacaa
    16561 agggattata tcaattaaag aacaagtaga aaatctagga ggaacgataa gtgtttctga
    16621 taatatacct catggagttt gtgtacagat tacaatccct atgaaaggag atggttctta
    16681 taaatatttt gttagttgat gaccatatgc tttttgctaa aagcttgtca attgcactag
    16741 aagaatacaa agaaatcgaa cagttttatt ccacacagga catccataat ctttcagaaa
    16801 tgattcaaga taaaaaaata gacattgtct taatggatat aaaccttaga ggcttaagtg
    16861 aatgtgatgg cttaaaagta gcttataatt tattagagac taattcaact attaaaataa
    16921 taatcttgac tggatacgac ctcccagttt ataaacatga ggctaaaaaa ataggtatct
    16981 atggattcct caataagaat attagccctg atagtttaat tgattcatta ttaaaagtct
    17041 atcaaggggt aacttgtttt ccatctgaaa atactgaagt aattattgaa gatttaacta
    17101 ctatggagaa aagaatttta cagcttatct gtagtggtaa aaaaagaaaa ataattgctg
    17161 atgaattgta tataagtgaa agaacattat caaatcattt acaacacata tatgacaagc
    17221 tagatgtttc atcatcagtt gaggctatta caaaagctat taaaatgggt tatatttctc
    17281 cagtatatta aaaaaagtga gtgtctcaaa ataaatcttc taactcataa agcactctcc
    17341 tttgtattaa atcaaatata ttttcatcat ttaaacaatt aagaagaggc ttctctctat
    17401 tttgagacaa ctccatttta atacactatt tatatttata tataaacata ttttaaacaa
    17461 ctaaaaagca actaatatct caatacatat cttcataaca catatcacca taattacaag
    17521 cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac agaattgttg
    17581 gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc tctataacct
    17641 tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt gccttagtta
    17701 tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt ataataattt
    17761 catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca tccgaatttt
    17821 caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat tcttttttct
    17881 ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct ttgtctatac
    17941 tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac tcatttctat
    18001 gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat ttctctatat
    18061 ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca gaacaaacat
    18121 aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc aatataccta
    18181 aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca aggtcagatg
    18241 cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt aatctttgta
    18301 tattttcttt tctcattaca tagtgattaa accttattct atttacatct cttttttcat
    18361 taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc ccactcctcc
    18421 aatttaaata gataatttgt tgtaaagttt ttagattttt aaataactta ttaaatgatt
    18481 atttttatta aaaagtattc tttgatattt tcattttaaa gtt
//
LOCUS       DAMJZE010000075        18464 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJZE010000075 DAMJZE010000000
VERSION     DAMJZE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.075"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            291..635
                     /locus_tag="PC541_002131"
     CDS             291..635
                     /locus_tag="PC541_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2839596.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            666..2267
                     /locus_tag="PC541_002132"
     CDS             666..2267
                     /locus_tag="PC541_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2839597.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2286..3386
                     /gene="dprA"
                     /locus_tag="PC541_002133"
     CDS             2286..3386
                     /gene="dprA"
                     /locus_tag="PC541_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2839598.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3419..5506
                     /gene="topA"
                     /locus_tag="PC541_002134"
     CDS             3419..5506
                     /gene="topA"
                     /locus_tag="PC541_002134"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2839599.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5671..6456
                     /gene="codY"
                     /locus_tag="PC541_002135"
     CDS             5671..6456
                     /gene="codY"
                     /locus_tag="PC541_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2839600.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6563..7828
                     /locus_tag="PC541_002136"
     CDS             6563..7828
                     /locus_tag="PC541_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2839601.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7859..8629
                     /locus_tag="PC541_002137"
     CDS             7859..8629
                     /locus_tag="PC541_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2839602.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8738..9184
                     /locus_tag="PC541_002138"
     CDS             8738..9184
                     /locus_tag="PC541_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2839603.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9202..10395
                     /gene="nifS"
                     /locus_tag="PC541_002139"
     CDS             9202..10395
                     /gene="nifS"
                     /locus_tag="PC541_002139"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2839604.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10449..10889
                     /gene="nifU"
                     /locus_tag="PC541_002140"
     CDS             10449..10889
                     /gene="nifU"
                     /locus_tag="PC541_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2839605.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11178..12257
                     /gene="mnmA"
                     /locus_tag="PC541_002141"
     CDS             11178..12257
                     /gene="mnmA"
                     /locus_tag="PC541_002141"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2839606.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12400..12638
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12706..15345
                     /gene="alaS"
                     /locus_tag="PC541_002142"
     CDS             12706..15345
                     /gene="alaS"
                     /locus_tag="PC541_002142"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2839607.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15370..15627
                     /locus_tag="PC541_002143"
     CDS             15370..15627
                     /locus_tag="PC541_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2839608.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15637..17808
                     /locus_tag="PC541_002144"
     CDS             15637..17808
                     /locus_tag="PC541_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839609.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18014..18439
                     /gene="ruvX"
                     /locus_tag="PC541_002145"
     CDS             18014..18439
                     /gene="ruvX"
                     /locus_tag="PC541_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2839610.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
BASE COUNT         7574 a         1882 c         3493 g         5515 t
ORIGIN      
        1 atagttatga ccggatacaa tgtattttca acatttttca aatagcttac aactaatata
       61 aataaagata aatatcgtat aaaagcaact aatttttctc tgttcattaa aggcactcct
      121 ccaaaaaaat aaacttttcc tatgaaaact agaataatat cataaaagaa taaagtcaat
      181 agaagtattt attaatattg atggaaataa aatcaatcca aaaacttgaa tagtaaaatt
      241 gtatactgac cataatttaa aagtgaaaat ttaatgtatt ggagtgaata atgaataata
      301 aagaaaaagg tgattttgga gaaaaagtag cagttaatta tttgctatca aaaggagcaa
      361 agatactaga aaaaaattac agattaaaga taggggaaat agatataata gcaaaaatgg
      421 aagatgaaat tatatttgtg gaggtgaaat caaggtctaa cataaagttt ggctaccctt
      481 gtgaatctgt aagcttcaaa aaaagaaaaa agataattgg agttgcaaat tattatatta
      541 taaaaaataa cttaaataac actcccataa gatttgatgt tatagaggta tatttattag
      601 aaaaaagaat aaatcacata atgaatgctt tttaaaaaag ataatgtttt tggaaaggat
      661 taaatatgtt aagtattatt aattcaagca acttagtagg aatcgacagc tttttagtta
      721 aggttgaagt agatgtaagc aatggaatac ctagttttaa tattgttggt cttccaggca
      781 aagaaattaa agaagcaaga gaaagagtaa agtcagcaat tcttaatagt ggatataaat
      841 ttccaagcac gagaatagtt gtaaatttat caccagcaga tataaaaaaa gaaggagctt
      901 ttttagattt atctataagt ataggccttt taagagagtt aataaaaaaa gatgaaaatt
      961 acataagaga aagtatgttt ataggagaat tatcattaga tgggaaaata agaaaagtaa
     1021 gaggtatatt gcccataatt atgggtgcaa aaactcagaa cataaaacgt atttttatac
     1081 caattgagaa cataaaagaa agtctatttg tagatgaaat tgatataatt cctattaaaa
     1141 gtttaaaaga atgtgtcgat tttttaaacg aagagataaa agtcgataaa gtaaggataa
     1201 tgtctttttt ggatgataag tcaagaaaag agaacgaaga attagaaaaa gataatagtt
     1261 atatagattg caaatatact aagattaata atgaagaaag taaatatgat gaagacttta
     1321 aagatgtaaa aggaaattat tttgtaaaga ggagtgcaga aattgctgct gctggaaatc
     1381 ataatatgtt tatgattgga cctccaggct caggaaaaac tatgattgca aaaagagtaa
     1441 ggacaatttt accagatata agtgtagaag aaatgataga agtaagtaag gtctatagta
     1501 tattgggtat gataaatgaa actaaaggaa taattgataa aagacctttt agagcacctc
     1561 atcatacaac tacaaagcaa tctttaattg gaggaggaat ggatgcaaga cctggagaaa
     1621 ttgctttagc acatagaggt atcttatttt tagatgagat agccgaattt gatagaaaaa
     1681 tacttgagac tttaagacaa cctatagaag atggatatgt aaatatatcc agagtaaagt
     1741 acagtgcaaa atatccttgt agagtactgt tagttgcagc tatgaatcca tgtccatgtg
     1801 gttattatat gtcagagaca gagtgtagat gtagaagtaa cgaaattgat agatacataa
     1861 ataaaatatc aggtccattg ttagataggt ttgatatttt tgtagaagtt aattctataa
     1921 aatattcaga ttttaattct ttaaaacaag aagaaagctc tcaaaaaata aagagaagag
     1981 tagaaaatgc tagaaaaata cagataaata gatttaagaa agataatata aaaaataata
     2041 gtgaaattaa agcttacaat ttatttaaat attgtaaact ggaaaaagaa gcttcaaaaa
     2101 cggctgagat gatatttagt aaatacaacc taagcagtag aagttataca aaacttttaa
     2161 aaatggcaag aacaatagca gatttagaag aaagagattt gataaattca cagtgtatta
     2221 tagaagcttt ttcatttaga aaggcttatt atagttattt taaatagttt tttgaggtgg
     2281 attttatgga aaaaagagat acttatttat ggttaaaatc aattggtgga ataacaacaa
     2341 aaacaattga aataatagaa aatgaaatag taaatattga agatatattt gatttttctg
     2401 aaaaagaaat atataattta aaaaatataa gcttgaatat tagaaaaaat atagtaaaat
     2461 atagaggtca cgcttattta gaaaatataa aagaactact ttacaaaaaa gcgattaaat
     2521 atatttgtaa atatgataaa gaatatcctg aaaatttaaa gaatatatac aacgctccaa
     2581 agttattatt ctacaaaggt gacataggct tagttaataa taattttaat atagctatag
     2641 taggttcaag aaaacctaca gcctatggaa ttaattgtgc aaaaacaata agttgtcaac
     2701 tatcccaata tggagttaat atagtaagtg gattagctat aggaatagat gcatactcgc
     2761 acataggttg tatgagtgga aaatctaaaa ctatagcagt acttggttca ggagtagaca
     2821 atcctcttcc aaaacaaaat ttacatttat caaataaaat attagaaaat gggggattat
     2881 tattatcaga gtataatatt aactcgacag tagccccgta ccatttttct aatagaaata
     2941 gaattatcag tggtttaagt gatggagtag ttgttgtaga agcagcaata aaaagtggtg
     3001 cactaatcac agtagatttt gctcttgaac atggaaaaaa tgtttttgca atacctggta
     3061 atataaattc tcaaatgagt agaggttgtc ataaaattat aaaagaaggt gcaaaattaa
     3121 ttgaaaatat agatgatatt ttaaatgaat ataacatatt taatattatt gataaaaaaa
     3181 taaatcaaaa atatgataat ataagtctaa atgccaagtc taagcaaata attgaagcta
     3241 taaaaagaga aggaaacttg catatagata gtatttgtga ttatactggt atagaaataa
     3301 agtatgtaaa ttcaataatc aatgaattag tactaaatga gttagtagta gaaatgaata
     3361 ataaaacgta cagtttaaat gtataaatta agattaggta gtgggggtgt atggttttat
     3421 ggccaaaaca ttagtcatcg tagaatcgcc tgcaaaagct aaaactatag aaaaattttt
     3481 agggaaaagt cattacacag ttaaagcatc agttggtcat gtcagagatt tacctaaaag
     3541 taaattaggt gtagatatag aaaataattt tgaacctcaa tatataaata taagaggaaa
     3601 aggcgatgta ataaaagaat taaaaaaaga agctaaaaaa tctaaacaag tatatctagc
     3661 aactgaccct gatagagaag gtgaagctat atcatggcat ttagctcata tactaaattt
     3721 agatgaatct gaggattgta gaatagagtt taatgagata actaaggatg caattaaaaa
     3781 agcaattaaa catcctagaa atattgatat aaatttggtt gatgcacaac aggcaagaag
     3841 agtgctagac agacttttag gttatcaaat aagtcctata ttatggcaaa aggttagaaa
     3901 aggtcttagt gcaggtagag tgcaatctgt tacaacaaag ttaatttgtg atagagaaaa
     3961 agaaataaag gcatttattc caaaagaata ctggacaata gatgttgagg ctaagacagt
     4021 aaaagacaaa gatataactt tgaaatttta tggtaaaaat gatgaaaaga ttgaattaga
     4081 aaatgaagaa gctgtaaatg aaatactaaa agaaatggaa ggtaaagatt taaaggttga
     4141 gaagatagaa tctaaatcaa gacgtaaatc agctccaaaa ccatttacaa caagtatgct
     4201 tcaacaagaa ggagcaaata agttattttt tactactaaa aaaacaatga tgatagcaca
     4261 agaattatat gaaggtatcg acatagaagg tgaaggtact gttggtctta tatcatatat
     4321 aagaactgac tctaaaagaa tatctgatga agcaaaagaa aaagctaagg gttatatcgt
     4381 aaacaattta ggagaaaact actataaaaa cattggtgat aaaaaagaaa aaaaggatac
     4441 taaaaaggtt caagatgccc atgaggcaat aagaccaacc tcagttgata gaactcctga
     4501 gagtataaaa gcctcattga gcaaagacca gtacaagtta tacaatttaa tttggagaag
     4561 atttgtagct agtcaaatgg aagattcagt gtttgatata ctaaatgtag aatgtaaaat
     4621 aggcgatatt gtatttaagg caactggttc aaaaatgaaa tttgatggtt ataccaaggt
     4681 atataatttt tcagaaagag aagacaaaat tttaccaagt atagaaaaag gagatatatt
     4741 aaatatagaa aatatattgc cagaccaaca ttttacacaa ccaccagcaa gatatacaga
     4801 agctagttta gtaaaaacac tagaagaact aggtataggt agaccaagta cgtatgctcc
     4861 aacaatagct actatactga atagagagta tgttgaaaag caaggaacaa gtctatgtcc
     4921 aacagaactt ggaataatag ttactgatat tttggaaaat aatttccata aatttataga
     4981 tgtagatttt actgctgata tggaaagtaa acttgatgtt atagaagaag gcaatacaga
     5041 ttggaaaaag attgtatcag aatcatatgc accacttaaa gaggctattg aagaagctct
     5101 tgaaaatata gaaaaagtaa atatggatga agaaactgat gagatttgcg agaactgtgg
     5161 ttctaatatg gttatcaaat atggtagatt tggaaagttt atggcttgta aaaactatcc
     5221 cgattgtaaa acaactagac caattatgaa taaaattggt gttaaatgtc caaaatgtga
     5281 agaaggagat attatactta gaaaatctaa aagaggtaaa gctttttatg ggtgttctaa
     5341 ttatccaaac tgtgactttg tagcttggaa taagccaaca ggagagtttt gtgagaagtg
     5401 tggttcatat atggttgaaa aagtgactaa atctgaaaca aaaacagtgt gttctaataa
     5461 agaatgtaat aaagagacaa aagagcataa tgacggaaaa gagtgaaaat tgaaaaaaat
     5521 cacataattc tcttgaaaaa ggttccaatc tatgataatc tttcaagtgt aaacaactaa
     5581 tagaaaaata aattttcatt aaatgtgaaa ataatattag aaaatataaa aggttattaa
     5641 aaggcctaaa tttcatgagg agatgattaa atggcaagtg aagtgttaca aaaaacaagg
     5701 aaaataaata aaacattaca aacaagtggt ggaagcagtg tctcttttga tttactggcc
     5761 ggagcattgg gcgacgtttt aagttctaat gtttatgtag taagtgcaaa aggtaaagta
     5821 ctaggtcttc atttaaatga tgttcaagac agttcagtta tagaagatga gtatactaag
     5881 caaaagaaat tttcagatga atatactcaa aatgtgttaa aaattgatga aacattagaa
     5941 aatttaaatg gtgagaagat attagaaatc tttcctgaag aacatggaag attacaaaaa
     6001 tatactacag tagttccaat attaggaagc ggtcaaagat taggaacatt ggtactttca
     6061 agatattcaa attcattcaa tgatgatgat ttagtaatag ctgaatacag tgcaactgtt
     6121 gttggtcttg aaatattaag agcaataggt gaggaattgg aagaagaaat gagaaagaaa
     6181 gctgtagttc aaatggcaat aggcactctg tcctactccg agcttgaagc agttgaacat
     6241 atttttgctg aattggatgg aaaagaaggt ttacttgtag caagtaagat agctgataga
     6301 gttggtataa ctaggtctgt aatagtaaat gcacttagaa aatttgagag tgcaggtgtg
     6361 atagaatcaa gatcattagg tatgaaaggt actcatataa gaatacttaa tgacaaactt
     6421 acagatgaat taaaaaaatt aaaaaacaat caataatcta tattttatag gtttagatta
     6481 gaaaaggtat ctatttcaaa tagatacctt ttttagacga attattaagg taatgataag
     6541 tatattaaaa aggagtgaga aaatgccttt agcagataaa ataagaccaa aaacaatgaa
     6601 tgatgtaata ggtcaaagtc atataatagg taatggaaaa atattgtcta agatattaca
     6661 atctaatttt ttaccaaata tgatattttt tggtcctcct ggtgttggta aaactacagt
     6721 agcggaaata atagcagaaa gaagcaataa aagcttctat aaaataaatg ctactaattc
     6781 atctttagaa gatataaaaa aggtgatatc agagcttgga agtataaata atgtaaatgg
     6841 agtcctatta tatatagatg aaatacaaag ttttaataaa aaacagcaac aatcaatatt
     6901 agaatttatg gaaaatggga gtataacttt gattgctagc actacagaaa atccatatca
     6961 ttatgtatat aaagctcttt taagtaggtc tactgtattt gaatttaaac ctttagaaaa
     7021 acttgatata gaaaaaggac tgaaaagagc tgttgaagta ttaaatgaag atagctatat
     7081 ggatattgag tgtaataatg atgctattga agatatagca attctttctg atggagatat
     7141 gagaagagcc ttaaatattt tggaagtggt agtctattca actaaaccaa ataaggataa
     7201 tataacttat atagattcag atgtaataag ggcatctaca tttaataaaa ttataaacta
     7261 tgacaaaaat ggtgatagtc attatgatat tttaagtgca tttcaaaaat ctataagagg
     7321 aagtgaccca caggcatcta tacactatct agcaagactt ataaaaggtg gggatttgat
     7381 aagtatatgt aggagacttt tagtaatagc tagtgaagat ataggtttag cttatccaaa
     7441 tgctattgta attgtaaagg catgtgtaga ctcagctatg caacttggat ttccagaagc
     7501 gaaaatacca ttagcagaag ctactattct tttggctaca tctccaaagt ctaattctgc
     7561 atgtatagca ataatgaaag ctatggatga actggataaa gagtttgtta aggatatacc
     7621 aaatagtata aaggatgctc actattctgg ttctaaagaa ataggtaggg gttgtggata
     7681 caaatatcct cataatttta aaaatcatta tgtaaagcaa cagtatttac cagatagcat
     7741 aaaagatagt atttattaca taccacaaga aaataaaact gagaaaaata taaaaaaata
     7801 tttagaatat ctaaatagtg atttttagac tgaactttat gtatcggggg tagatattat
     7861 gattataaaa cttaactcaa cttatcacag tagagttatg aaatacttaa aaaaagaacc
     7921 tgagtacaat ttgtttataa taggggatat tgaaagatat ggatatggaa ataatttttt
     7981 aaatatatgg gcagatgtgg gtgaacatgg tgaaataaag gcaatattac ttaaatattt
     8041 tgaatttatg atgttttatt ctgatggaga gtatgatgtt gaaggttttt ataatctact
     8101 tcgtaatact aattacgaag aaatatctgg gaaaatatgt gctgtagatg ctttagcaaa
     8161 gagattagga cttaataatt taaaggtagt tgatttttgc aaacttcaaa ctaaaaagtt
     8221 tttgatagat aataattgta atgcaaaggt taagagaatt aggcttggaa atctaaaaaa
     8281 gacagtaaaa ctttatgact taatagatga atttcacagt acaactttag agaatttaaa
     8341 aaatggacta aggacaggta gaggatattg tatagagatt aacaaacagg tggtgtcaat
     8401 ggcaaagagt acttcagaaa ataggactca tgcaatgata attggagtag gaacacatcc
     8461 taaatataga gctaaaggac ttgctacaaa atgcctaatt aaactatgtt cagagttact
     8521 gagagaaaac aaaattcctt gtttgtttta tgataatgaa gaggctggaa agatatataa
     8581 aaaactagga tttgaaaaca ttggaaaatg gggaatttat tcaaaataaa aaattaaaat
     8641 taatcttgac aaaattagta gggtataata aaattatctt gaaagtaatt cctactaaaa
     8701 cagtatgaat tagatttcta tatagaggtg ataatatatg aagttgtcta ctaaaggtag
     8761 atatggacta aaagcaatgt ttgaattagc attaaatcaa gacaatggac cagtatcatt
     8821 aaaatttatt gctaagaagc aaaaaatatc agatcagtac ttagaacaaa tattttcatc
     8881 tttaaaaaaa tcgggtctag taaaaagtgt gagaggtgct caaggaggtt atttgctttc
     8941 aaagaatgct gaagacataa cagtaggaga tatacttgta gtattagaag gaccagttgc
     9001 attatctgac tgtgtactag atgaagatgt atgtgaaaac tcaaacatgt gtgtaacaaa
     9061 aatagtttgg gaaaagatga aaaagggtat agaagatgtt atagattcaa ttactcttaa
     9121 agatatgata aatgactata acaaaaataa attagaaaat gatataacaa atattaaaaa
     9181 atagatttgg agatgaatgt aatggaaaaa agaagattgt atatggatta ttctgcaaca
     9241 acacctatta aaaaagaagt attggatgca atgatgccat acctaacaga ttattttggt
     9301 aatgcatcta gttttcacac attcggaaga gaagcaaaag atgctttgga taaagcaaga
     9361 gaacaagtag cagcccttat aaatgctgaa cctagtgaaa tatattttac agcaggtggt
     9421 tcagaaagtg ataactggac actagagggt gtagcttatg ctaataaaaa caaaggtaat
     9481 catataataa cttcaaaaat agagcaccat gcaatacttc atacatgtga atacttagca
     9541 aaacatcatg gatttgaaat aacttattta gatgttgata gcgaaggaaa agtagactta
     9601 aagcaactag aagattcaat aaaagatact actatactta taagtataat gtttgcaaat
     9661 aatgaaatag gaactataca gcctattaaa gaaatatccg aaatagctaa aaaacataag
     9721 atattattcc acactgatgc agtacaagct acaggtaata ttccagtaga tgttaaagaa
     9781 ttaggaatag atttgatgag tatgtcttca cataaaatat atggaccaaa aggtgtagga
     9841 gctttataca taagaaaagg tgtaagactt cataactttg tccatggtgg agcacaagaa
     9901 aaaagcaaaa gagctggtac agaaaatata cctgcaatag ttggatatgg aaaagcagca
     9961 gaattagcta aggaaaatat gcaaaatcat gtagaaactt taactcgtct tagaaataag
    10021 ttaatagatg gtgtgttaga aagaatacct tacacaagag taaatggtag cttagaaaat
    10081 agattgccag gaaatgcaaa ctttgcattc caatttatag aaggtgaagg aatactttta
    10141 ttattagata tgttaggaat tgctggttca agtggttctg catgtacttc tggttcatta
    10201 gacccttcac atgtacttct tgcaataggt ttaccacatg aaatagcaca tggttcatta
    10261 agacttacag ttggagattt tacaacagat gatgatatag attatatact agagaactta
    10321 ccaaaagtaa tagaaagact tagaagcatg tcaccacttt atgatgatgc gaagaaacaa
    10381 ggtttagtaa aatagacata aagataaagc ataaaaaata gacataaatt gagtaggagg
    10441 aaaagataat gcaatatagt gataaagtta tggaacattt tatgaatcca agaaatatgg
    10501 gagaaattga taatgcaagt ggtgtaggtg aagttggaaa ccctacatgt ggagatataa
    10561 tgaagatatt tttagacata gatggagatg taataaagga tgttaagttt aaaacatttg
    10621 gctgtggttc agctatagca agttcttcta tggcaactga aatgataaaa ggaaaaacta
    10681 taaaagatgc attagaactt acaaataaag ctgttgcaga agctttggat ggtttaccac
    10741 cagtaaagat gcattgttca gttttagctg aacaagctgt aaaagcagct cttatagatt
    10801 atgctcaaaa gaacaatatc catataccag aattggatgg atatgttata gatgatgctc
    10861 atgaccatga tgttgaagaa gaagaataaa aataagtgat tgtgttatga gaagtttaca
    10921 atgattaaaa agaagtacta atgatataat taggacataa aaaatagctg tttttaaaat
    10981 agctattttt tattgcctaa attaagtatt ttaacttgat ttagaggtta attagttaac
    11041 aaaataatcc ataagattca aataataaag atgattattt ttttagaaaa tagaacaagc
    11101 tatattttaa ataagtacaa tacttgctct atatggtata ataataagta atgcaaaaga
    11161 tgaggtgaaa tttgtttatg aataaaaaag taatgatagg tatgagtgga ggagttgata
    11221 gctctgtagc tgcatatctt ttaaaacaac aaggatatga tgtcattggt gttactatga
    11281 agttgtggca agatgatgat gttgttgaaa ttgaaggtgg ttgttgctct ttatctgctg
    11341 ttgaagatgc tagaagggta gcaaataaaa taggtatacc attttatgtt ttaaatttta
    11401 gagaagtgtt taaagaaaaa gtaatagatt attttataga tgaatatttg gaaggaaaaa
    11461 ctcctaatcc ctgtatagct tgtaataaac atataaaatt tgatgatttt tataagaagg
    11521 ctagacaaat aggttgtgat tatgtagcta caggtcacta tgcaaagata gaaaaggatg
    11581 aaagtacagg aagatatttg ctaaaaaaat ctgttacaga taaaaaagac caaacttatg
    11641 cactttataa tcttacacaa gagcaactag agcatacttt attgccaata ggtgactatg
    11701 aaaaagatag agtaagagaa attgctaagg aaatgggtat ggcagtacac aataagcctg
    11761 atagtcaaga aatttgtttt gttaaagata atgactatgc aaattatgta aaaaaacatt
    11821 ctaaaaagcg tattgaagaa ggtttttttg tagatactaa aggcaatata cttggaaaac
    11881 ataagggtat attatattat actataggcc aaagaaaagg gcttggaatt acttttggaa
    11941 aaccaatgtt tgtaatagac ataaatccaa taaataatac catagttctt ggagacaatg
    12001 aagatttatt taaaaaggaa cttattgcta aagatgttaa ctttatatca atagatacgt
    12061 tagaagaacc cttaagagtt caagccaaaa taagatattc agcaaaacca tcaccagcta
    12121 ctattcatag agtaggagaa gatactatta aaatagtttt tgatgaagct caaagagcta
    12181 ttactaaagg tcaatctgta gtaatgtatg atggagatat tgtagtaggt ggaggtataa
    12241 tcgagaaaag tttataaaaa catctaaaaa attaaaaaaa taagcaataa agtatattaa
    12301 gagtaaataa agtttataaa ataaatttat tgtatatgaa aatatagtat tgattaattt
    12361 tgtaaaattt agtaatataa ttagtatatt aattaaaaaa caaatacagt gataaggaat
    12421 agtaaaatat ttgatgtctt acagagagag ggaaatgctg gaagccctta gaatatgata
    12481 tttgaagcag ccttttagta gcaatcttga acaatttatc ttttataaag aagatttaag
    12541 tagagattga cggtagttac gttatagcta aggttttccg aagatatcat actttgtatg
    12601 ataattaggg tggtaccgcg attgtcctcg cccctataaa tttatttata gggacgaggt
    12661 tttttattta aacgaaaaaa taaaatttat taggaggaac atgatatgga aaagatggga
    12721 ttaaatgaaa taagaagtaa gtttttagaa ttttttgagt caaaaggtca ttatgtagca
    12781 aatagttatt ctttagtacc aaacaatgat aagagtttgt tacttataaa ctcaggtatg
    12841 gcacctttga aaaactactt ttcaggtgta gaagtacctc caagtgtgag aatgtgtaca
    12901 tctcaaaaat gtataagaac aggagatata gaaaatgtag gtataactgc aagacatgct
    12961 acattctttg aaatgatggg taatttctca tttggagatt actttaaaag agagtcaata
    13021 aaatggggat gggaatttgt tacagaatgg ctaaatatac cagaagataa gatatgggtg
    13081 actgtatatg aagaagatga tgattcttat gatatatggg caaaagaaat gaatttccca
    13141 gaagaaagaa tggttagact tggaaaagat gataatttct gggaaatagg tacaggtcct
    13201 tgtggtcctt gttcagaaat atattttgat agaggagaag aatatggatg tgacaatcca
    13261 gactgtaaac caggctgtga ttgtgatagg tatttagaat tttggaacca tgtatttact
    13321 caatttgata gagatgaaga aggaaattat agtctattag aaaacaagaa tatagataca
    13381 ggtatgggtc ttgaaagaat gggatgtatt atgcaaggcg tagacactat ctttgaagtt
    13441 gatacaatta aatctatact agaagcagtt gagaagttaa caggagtaaa atatggagag
    13501 aatccaaaaa atgatatatc aataagaata ataactgacc atataagagc tgtaacattc
    13561 ttagttagtg atggagtgct tccatcaaat gaaggaagag gatatgttct tagaagactc
    13621 cttagacgtg ctgctcgtca tggtaagtta ttaggagtaa aagaattatt tttacagaaa
    13681 ctaatagatg aagttataaa agtaaatgat aaagcatatc cagttttagt tgaaaaagaa
    13741 agttatatta aaaaagtagt tggaatagaa gaagaaaaat tcaatgaaac tatagaccaa
    13801 ggaacagaaa tattgaactc ttatatagaa gtattaaaaa atgaaggaaa gactgtccta
    13861 agtggtcaag aagcttttaa actatatgat acttatggct tccctataga cttaactaaa
    13921 gaaatattag aagaagaaca tctatctgta gatgaagaag cttttaatga agaaatggaa
    13981 aagcaaaaag aaagagctag aaatgctaga ggaaatatgg atggagaaag ttggaaggaa
    14041 gaccctctat caaaattaga atctacagtt gacagtacat ttaatgggta tagtgaaatt
    14101 tatggtgaag gaactattga agctatagtt aaagatgatg agctagttca aagtgcagaa
    14161 gaaggcgata aggtatctat agtactagat aacactactt tctatcctga aggtggagga
    14221 caagttggag attgtggatt gataactaat gaaaacttag ttttagaagt attaaatact
    14281 aaaaaaggtg ctaataatag tataaaacat ataggtataa taaaatcagg aagaataagt
    14341 aatggggaca aagtgaagac actcgtagat agagaaacta gaatgtcagc tgcaagaaat
    14401 cacagtgcta cacatttact tcataaggca ttaagagaag tattaggaga acatgttaat
    14461 caagctggtt ctttagtaac tccagaaaga cttagatttg atattactca ctttgaagcg
    14521 atatcaaatg aagaattaaa agtaatagaa gaaaaagtaa ataatgttat tttatcatct
    14581 ttagacataa aatgtgatat tatgaatata aaggaagcta aagaaaaagg agctacagca
    14641 ctatttggtg aaaaatatgg tgatgaggta agagtagttt ctatgggaga ttattctaca
    14701 gaactttgtg gaggaactca cttgacaaat acttctcaag taggaatgtt caaaatatta
    14761 tctgaaggtg gagtagcagc aggtgtaaga agaatagaag caataacagg aaaagctgtt
    14821 tatgaatacc taaaagaaag agatggaatt atctctgaag tttgtgtaaa cttaaaatct
    14881 aaagaagata atttaattca aagaataagt tctttattag aagaaaataa aaacttatct
    14941 aaagaattgc atgatatgaa agctaagatg agtttacaat cagttgattc tatttttgat
    15001 tctaaagttg aagtaaatgg agttaatttg ataactaata aatttgaagg tatggatatg
    15061 gatacactaa gagaaacagc agataatcta agagataagc ttggttctgg tgtagtagta
    15121 cttgcaaatg tagtagatga taaagttaac tttgtagtta ctgccactaa agatgtatta
    15181 gataagggaa tacattcagg taatattgtt agagaagttg caaaaatagc tggaggaaaa
    15241 ggtggaggaa gacctaatat ggcacaagca ggagctagtg atgtatctaa ggttgaccaa
    15301 gctttaagct atgcaagtga ggttattaag acacaagtta aataacattc actcaaggag
    15361 gtttttagtt tggaaaaaga tttagactac actatgaaat ttgaaggaat accagaagat
    15421 aggatgagtg taggagatac tattgatttt gtatataaag cattagttga aaaaggatat
    15481 aaccctataa atcaaattat agggtatctc ctttctggtg attctagcta tataactagt
    15541 cataaaaatg caagagctat aattaaaaaa tttgaaagag atgagatact tgaagaagta
    15601 atcacgcact atctgaatag aaagtaggtc aaataaatga gaaaaaagat tatgtcgttg
    15661 tttgcaatag tcattatgat ttttgtatca actgctagct atggttttgc tgaagaaaaa
    15721 ggcaaggtca tatttattga tatgaatagg accaatcttg gaagtatgat gaatattcca
    15781 atcttgaaaa atgagataga aaagagaggc tatgtagctc tcatgaatat aagaggggat
    15841 caaggtactg atgatagaag gtcttatgca agtatgggtg caggaacaag agctagtgta
    15901 actactgaag actatatcaa ttttcaaaaa gtagaccaag atactgctaa agcttttgaa
    15961 gtcgcaacag gtcaaaaagc taaagaaata aatgatttag gcattaatag atctataaat
    16021 gaaaatcttg agtatggaga atatggagca aatttagggt cattaggaaa aactttatca
    16081 gaaaatggtt taaagacatc tgtaattgga aatgctgata taattgaaaa taatcaatta
    16141 gttaaaaaca gaaacttatg tttagtagct atggaccaat atggaaggat tgattcaggt
    16201 aatattgata atattaataa gaaagattct aaaatgccat ttggaatatc tacagattat
    16261 gataaattac tagatgaaac taaaaaatcc tataaaaata gtgatgctat atttgtagaa
    16321 cttggagata catatagatt agatatgtat agattaaatc taaatgataa tacttatgat
    16381 ataatgaaga aaaatattgt aaaaaatata aataaatatc ttgaacaagt attttctatg
    16441 gttggagaaa atgacactgt ttatatagca agtgcatttc caagtgacct agattataag
    16501 aataagagaa gactttctcc aataataaaa tttagtggag aagggaaagg tctactggaa
    16561 tctgccacaa caagaaaggc tggaattgtt gccaatttag atgttggagt agatatacta
    16621 ggtacatttg gtcttgaaaa taaagagatg gtaggtagaa gttatacttt agtaaataaa
    16681 gaggacaata ttagctatct aaaatcactt tatgaaagga tagtgtctat atcttcaata
    16741 aggtcaacta ttataaatgg ctttgtaggc gtaatatctg cttcttgggt aattgccatg
    16801 tttgcgattt tctttagaaa ttatatgcca cataaagagc gtgtattcaa agtacttaag
    16861 gagtttataa aattaggcat aataatgcca ctatcattta tgttagctcc tttgatgaat
    16921 tttaaaactc caatttcaat atctttggga atatttgcaa ctacgctaat attatacttg
    16981 ttaggtagaa aattatttaa ggacaatgat ataaagcaca tgggattttt tgcaacactt
    17041 actattttag tcatagttat agattctgta tttggaacat acttaatgca aaataacata
    17101 atgagttatg atgctgtcat aggagctagg tactatggtg taggaaatga atatgaaggt
    17161 gtaactatag caagtgcagt atttggctta gcagtattat tacattataa gaaggtatct
    17221 aaattacttg ttgtaatttt ttcattaata atacttataa ctagtgcata tccttcaatg
    17281 ggtgcaaatg ttggtggagc tatatcagaa tgtgtagcat atttgttgtt tataatgctt
    17341 atatatgaca ttaaaataga ttttaagaaa gctgtattat tagctgtttc agctgtagtt
    17401 gtcgtttttg catttgcagc tttagatata ctttcaggta gtgaatctca tttaggtatg
    17461 tttgtacaga gaattttcct aaatggacca ggagagatat tacaaacatt tggaagaaaa
    17521 attcaaatga atatgcaact agcacagaca agtgtatggg taaatatttt acttgtagga
    17581 attggagtaa ttgcagtgct tatatttaaa ccatctagac actttagaaa gataatgaat
    17641 aattttccaa tcttgtttaa agggtttatt gcatctatgg ttggttgtat aattacactt
    17701 ttagttaatg attctggaat agttgctgca tctacagcat ctatatatat acttatacca
    17761 ctaataataa taagtataaa tatgataata tttgaaaaaa ataactaaaa aaatttttac
    17821 tgttcatata ggatttaaat aaaaaatgca atatgattat taatgtaaac tggatgctta
    17881 cataaaaaat catattgcaa tttttatgat tgattatgta aaattatatg aagcagtaag
    17941 cctgtgacct tagttatgag aggttcagaa tatgtattta atctatagaa tataaataga
    18001 taaaggagga actatgttag atggaagaat aatgggtctt gatgttggtg ataaaactat
    18061 tggagttgct gtgagtgatt tgatgggatt aactgcacaa ggtgtaaaaa caataaaaag
    18121 agttggcaag aaaaaagata ttgaagagct aaaagcaata ataaaagaaa aacaagtaaa
    18181 taaaattgta tcaggattac caaaaaatat gaatggaact ttaggtccac aaggagaaaa
    18241 agtaatcaag ttttgtgaat tggtaaaagc agaaacaggt attgatgtag agttttggga
    18301 tgagaggctt tctacagtag cggcagagag gtctttatta gaagcagatg ttagtagaca
    18361 aaaaagaaaa aaggttatag atatgttggc agcagtaata attttgcaag gatatttaga
    18421 ttttaaaata aattcataaa agaactataa aattgaatac ttat
//
LOCUS       DAMJZE010000076        18269 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJZE010000076 DAMJZE010000000
VERSION     DAMJZE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.076"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            174..1547
                     /locus_tag="PC541_002146"
     CDS             174..1547
                     /locus_tag="PC541_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839611.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1803..2870
                     /locus_tag="PC541_002147"
     CDS             1803..2870
                     /locus_tag="PC541_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2839612.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3014..3322
                     /locus_tag="PC541_002148"
     CDS             3014..3322
                     /locus_tag="PC541_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2839613.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3315..4745
                     /locus_tag="PC541_002149"
     CDS             3315..4745
                     /locus_tag="PC541_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2839614.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4823..5197
                     /locus_tag="PC541_002150"
     CDS             4823..5197
                     /locus_tag="PC541_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2839615.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5191..7395
                     /locus_tag="PC541_002151"
     CDS             5191..7395
                     /locus_tag="PC541_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2839616.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7395..8804
                     /locus_tag="PC541_002152"
     CDS             7395..8804
                     /locus_tag="PC541_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2839617.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8789..9853
                     /locus_tag="PC541_002153"
     CDS             8789..9853
                     /locus_tag="PC541_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2839618.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9883..11304
                     /gene="nhaC"
                     /locus_tag="PC541_002154"
     CDS             9883..11304
                     /gene="nhaC"
                     /locus_tag="PC541_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2839619.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11733..12197
                     /locus_tag="PC541_002155"
     CDS             11733..12197
                     /locus_tag="PC541_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2839620.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12431..12793
                     /locus_tag="PC541_002156"
     CDS             12431..12793
                     /locus_tag="PC541_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2839621.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13388..14248
                     /locus_tag="PC541_002157"
     CDS             13388..14248
                     /locus_tag="PC541_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839622.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14506..16620
                     /locus_tag="PC541_002158"
     CDS             14506..16620
                     /locus_tag="PC541_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2839623.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHAVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            17161..18114
                     /gene="blaCDD"
                     /locus_tag="PC541_002159"
     CDS             17161..18114
                     /gene="blaCDD"
                     /locus_tag="PC541_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2839624.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         7287 a         1972 c         3370 g         5640 t
ORIGIN      
        1 tatataaata aaattttaaa tatacttaaa aatatattaa tataatgtac ataaaaaata
       61 tcataaatat atgataagat aaatacaata gcaacatttt attaattttt tgggttttta
      121 tatatttagg tactggggtg atatagttga accaagagat attttttaaa ataatgaatg
      181 aaatattgga aaatattgat gaaggggtac attttgttaa taaagacggg aaaactataa
      241 tctacaataa ttctgtacaa aaaatggaaa aaatgaacag acacgatatt ttaaccaatt
      301 cattttttga tatagtcaac aaaatgaaaa taaacaggag tacactatta gaagttttgg
      361 aaaaaaggga agttatcaaa gacaatattc aaaaatatct ggataaaaat ggaagagaga
      421 taacagcaat taatactaca ataccaattg atattaaagg cgagtttgta ggggcacttg
      481 agatatcaaa gaatatgaca acgatagaaa atttactaaa cagaaattat aaaaaaaatt
      541 tagtcaataa agttagtatt agacaaaaaa agggttatga atttgatgat attttagggg
      601 aaagtaattt gattaaagaa gcaatagcta aatctaagaa agcttgcaag tctgatgctt
      661 cagtatttat atacggtgaa actggaacgg gtaaagagtt atttagccag tccattcact
      721 atggaagtaa taggaaaaac tatccattta tagcacaaaa ttgtgcagct ttgccggaat
      781 cactattcga aggtattctt tttggaacta gcaagggagg atttacagga gcaatagata
      841 ggccaggctt atttgaacaa gctaacaaag ggacattgtt attggatgag ataaattcca
      901 tgcctattat gttacaggct aaactattaa gggttttaca agaaggttat gtaagaaggg
      961 ttggaggaac taaggatata cctatagatg taagaattat tgcaacgaca aatgaaagtc
     1021 caaaggtaat tttaaacgag cataaaatgc gaaaagattt atattatagg cttaacataa
     1081 ttcatataaa aattcctcct cttagagata gaaaagagga catacctcta ttgtgcaaaa
     1141 aatttatttc aaagtataat aaaaagttaa ataaaaaagt agtaggaatt tcagatgaag
     1201 ctattaaaat tttaaaaaat tacgattggc aaggtaacat aagagagcta gaaaacataa
     1261 tttattgtac tatgagtatg atggaagatg aggtacaaat agaaacaagt atgattaatc
     1321 taaacgacta ttacaatctt aaagagaata aagagagtaa caattattct ttaaacgatt
     1381 tggaggacaa aacacttagt aagattatca gtgagataga agaaaagtat atatatgaat
     1441 cattagaaaa ggcatcttac aatataacta aagctgcatc aattttaggt atgaataggc
     1501 aaaatttaca gtataaaata aaaaaatata atatcaaaat attataaata agcaagataa
     1561 aaaaacaaaa tcaaagtaaa ataaaaaagc aaaatagagg agcaaaatta ttataaaaga
     1621 gcaaaatatt ttgctctttt ttttatttct cataagaaat attatttttt atttatgaaa
     1681 atacataata aaataattat agtttcaacg tttcaataaa tatttacttt aattttaaag
     1741 ttggcacgat ttatgcttta actaattatt gagaatatat aaatttttag gagggtgtat
     1801 atatgagaaa agtaagagta ggaatatggg gatttggagc tatgggaata ggtatggcta
     1861 atatgattct aaagaaagaa ggtatagaaa tagtatctgt ttgtagtaga agtacttcag
     1921 gaaaaagtat gtataatgtt ttaggaattg aaagaggaga aagaccagaa gttattatca
     1981 ataaaaatta tgaagaagtt tttagagaaa aaagtgtaga tgttgttttg ttagcaactg
     2041 attcatttac taaaaaggct tttgataaaa taattttctt attaaataga aaaataaatg
     2101 ttatatcaac agcagaacaa atggcatatc cacaagctga tgatgcagat ttagctaaaa
     2161 aaatggatga agttgcaaag gaaaatggag taagtatact tggaacagga ataaatccag
     2221 gatttgtatt agatttacta gtattggcgc tttcaggcac ttgtgaagaa gttactagta
     2281 taaaagctaa gagagtaaat gatttatctc catttggaaa gtctgttatg gaggaacaag
     2341 gtgttggagt aacaagggaa gaatttataa aaggtgtaga agataagact atagcaggtc
     2401 atgtaggttt tgtagaatca ataaatatga tagctgatgg attaggttgg aaattagata
     2461 agatagaaca aacaaaagag ccaataatga ctacagtaga tagaaaatct aagtacggag
     2521 aggcattggc tggaaatgtt gctggttgta gacaatgtgg atatggatat gtaaatggag
     2581 aagttttaat tgaaatggag catccacaac agataatacc agaagcagaa ggtattaaaa
     2641 caggagacta tgtatcaata aaaggtatac caaacattga tttacaaata aatcctgaaa
     2701 tacctggagg agtaggtact tatgcaatga tagtaaactc tattccatta ataataaatg
     2761 ctagaccagg tcttaaaact atgttggata taccagtacc tagagcaata atgggagata
     2821 taagaaatca gatagaagtt gaattggaag aagaatcaaa ggctaattag taagataaca
     2881 gctaactaat tagtaagata gcaactagct aattagtaag ataacagtta gctaattggt
     2941 aaaataatgt ttaatttaaa ataacagtaa attaattagt aaggcaatag tcaattaata
     3001 aggggatgaa aaaatggatg ctaaaattaa tgattgggtt attatacaca atatagtatt
     3061 gactccagaa gaaagagctc cacaagttcc agaggatact aagaaagtat ccttagaaat
     3121 gtgggttaaa ggttttatac aaagtgatgc ttctataggc gacttagtag aagttaaaac
     3181 aattacaggc agacttgtaa aaggtaattt acttaaaata aatccatatt atactcatga
     3241 ttatggtaaa tgtataccag agttactcca aataggaatc caagctaaag aaatattatt
     3301 tggaggtgta tataatgaat aattcaacat caatgaaaga tatgagttat caagctgtta
     3361 tgggaagaaa taacgaaata atgaaaaatg caatcggatt ggattattct tattttgaac
     3421 aagagggaat agggtttgat tatgagaaaa tgatgagtga aacaggatat acattacaag
     3481 atattgaagc aattcaatca cagtatgctg ttggaaatac tcctttaata gagcttaaaa
     3541 atttgactaa attagctaga aaatgtgcta aagaaggtaa aggagctaga atatttgtaa
     3601 aagatgaagc aatgaatgca tctggtagtt ttaaagcaag aagagcagct actgcagtat
     3661 atcatgccaa acaaatggga tataaaggtg taatagcagc tactagtggt aactatggtg
     3721 cagctgtagc atcacaagcg gcaatgcagg gattaaaatg tataatagtt caagaatgtt
     3781 atgattctaa tggtgtaggt caacctgaaa ttatagaaaa agcacgaaaa tgtgaggcat
     3841 atggagcaga agttgttcag ttaactgttg gaccagagtt attttatacg ttcttagttt
     3901 tattagaaga aacaggctat tttaacgcat cattatattc accatttgga attgcaggtg
     3961 tagaaacttt aggttatgag ttagctatcc aatttagaga aaagtataaa aaagaccctg
     4021 atatagttgt ttgtacaaat gcaggtggtg gtaatcttac tggaactgca agaggattga
     4081 taaaagctgg ttctattaat actaaagtag taggagcaag tgtagattta aaaggattac
     4141 acatggctag tgataatcaa tttaataaaa aatcatttac aacaggacat acaggttttg
     4201 ggataccata ctgtacatgg cctgataggt cagatgtacc taggtctgca gctagaccac
     4261 ttagatatat ggatagatat gtattagtaa aacaaggaga agttttttat acaactgaat
     4321 tattggcaca gctagaaggt atagaaagag gtccagcagg aaatacatct ttagcagcag
     4381 cattctcttt agctcaagag ttagatgagg ataaagttat agtggtacaa gaaacagaat
     4441 atacaggcgc aggtaaacat atatgtccac agcttacttt tgcaagagaa aatggaatag
     4501 atataaaatt tggaaatcca agagaagaaa ttgcaggagt aaacttaata ttaccagaaa
     4561 gaccagaatt attgaaatgt gttgatattg atatgaataa aataagaaag tcatttataa
     4621 aaaattgtat atctaataat cacattgata atattgataa gctaagtaat cgagatatag
     4681 aatttttaat ggaagaagta aaaagttcaa gagattttgt tgttgatgta ttaaataatt
     4741 tatagtaatt attgtttttt aatttaatat aaaataaaaa ttcagaaaat tcgattaaat
     4801 caatacgaat ggaggaatgt tcatgaaaaa aagagaggat gattttgaag ttagaagaaa
     4861 acatcttcaa gaactgagtg atgatgagtt aaaagaaagg ttttggagtt tagctaccca
     4921 gatagtagaa ccaatgatag aacttggtaa aaagaataca acaccatcaa tagaaaggtc
     4981 tgtcttgttg agaatgggat tttcttctct tgaagtaaaa cctattttag aaggggttat
     5041 ggaaagaggt cttataggta aaggagcagg gcatgtagtt tataaattgg ctaaatctaa
     5101 aaatataacg gttagagaag caggtttgtt attagttaag ggtgaatatt gggatgaagt
     5161 tacatgttta tttagagaag gggtagaatc atgttaaagg aaaataaaaa attggatata
     5221 gactatatat taaaagattt ggataaatat aaaccaaaac gaagaggttg ggtttggaga
     5281 gaacacttgg aaaatcttga aatgggacct tttaaatata aagactgtac aaaaccttta
     5341 aagaagagtg tagggctacc atcatctaag tatttcaata atatagaccc tcaaccaagt
     5401 ccagttatta ctactgaaat agcttctggt agatttgagg atgatataag aagaatgaga
     5461 atggcagctc atcatggagc tgaccatcta atggttatca gaactgctgg acaatcacat
     5521 tttgatgggc ttatagaagg aactccacaa ggtataggtg gagttccaat aacaagaaaa
     5581 caagtaagag ctcaaaggaa ggctcttgat tttatagaag aagaagttgg tagacctatt
     5641 aattaccatt catatgtaag tggtgttgct ggtcctgaag tagcagtaat gtttgctgag
     5701 gaaggagtca atggagcaca tcaagaccca caatataatg ttctgtatag gaatataaac
     5761 atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa taatggcttt tgccaatata
     5821 gctcaaattg atggagcaca taatgctaat gctactgcta gagaagcatg gaaggtaatg
     5881 cctgaactta tggttcaaca cgctttaaac tctatatttt cagaaaaaat aggtatagat
     5941 aaaagtaata tatgcttatc aacagttcca ccaacagcac ctccagcacc atgtttaaaa
     6001 atagacttac cttacgctgt tgcacttaga gagttattta gtgattataa gatgagagct
     6061 caaatgaata ctaaatatat ggaatcatca actagagaag caacagttac tcatgtatta
     6121 aacttattaa catcagtatt aactagagct gacatacaat caactataac acctgatgaa
     6181 ggtagaaatg taccttggca tatatacaat atagaagcat gtgatacagc aaagcaagct
     6241 ttagttggta tggatgggct tatggatatg atagaactta aagatgtggg tgaattaaga
     6301 gacaaagcaa gagaacttaa agaaagagct gtattatata tggaagaaat atctgaagtt
     6361 ggtggatatt ttgaatctgt ggaacaagga ttttttgttg attctggaaa ttatccagaa
     6421 agaaatggtg atggaatatc tagaaaaata aaaggtggag ttggtgaagg tactgtttat
     6481 gaaagagaag aagattattt agcacctgta actgctcact ttggatacaa taatgtagct
     6541 caatatgatg aaaatgctat agataatcca tcaatgttaa tagatggatg tacatttgaa
     6601 aatccagaca aaataatata tattgatgag cttgacgatt ttgataatgt agaaaataga
     6661 ctaaaagaat catatgagta cagaaatgga actaaaatta agcctgaaat ggaatggtgt
     6721 gcagatggaa ttgtaatgat aacaatgatg cttcctactg ataaaagaac agcagagttt
     6781 gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa ttgaggtcat aagtagagaa
     6841 gttatgcatg aatctgaagg gactagaata gaagtgaaag gaagagttcc gtttgatata
     6901 gatttaaaca atttagtaat accagaagaa cctaaggtac taactgatga agaaataaga
     6961 gcagatatag aagaaaaacc tatgaaaata gtttcagcta cggttggaga agatgaacat
     7021 tcagttggtt taagagaaat aatagatata aaacatggag gtattgaaaa atatggcata
     7081 gaatgccact atctagggac ttctgtacct gtagaaaaat tggtagatgc agcgattgaa
     7141 ctgaatgcag atgcaatact tgcatctaca ataataagtc atgatgatat tcactataaa
     7201 aacatgaaaa aattacacga ttattgtgta gaaaaaggta taagagataa agtaatgatt
     7261 gcgtgtggag gaacacaagt aacacctgaa atagctgttg aacaaggcat agatgctgga
     7321 tttggaagaa acagtaaagg catacatgta gccactttct tagttgaaaa aagaagagaa
     7381 atgagtaata aataatggaa aatagcaagg tttctttaaa aatagatgtg ttggtagctg
     7441 aaattggaag tactactaca gtggtaaatg ctttccacga tattaatact aacaatccta
     7501 tatttttggg tcaaggtcaa gctccaacaa cagtgtttga aggtggcgat gtaagaaatg
     7561 gcttatcagg tgctattaaa gatttagcta ataaattatc tgtagatgat atagagtata
     7621 atgatatgtt tgcaacttct agtgcagctg gtggattaaa aatgactgtt catggattag
     7681 tctatgatat gacagtaaaa gcagctaaag aagcagcttt aggggctgga gctgttattc
     7741 gtcaaataac atcaggtaga attaaaagaa ctgatttaaa taaaataaaa gaaataaacc
     7801 caaacataat tttaatagct ggtggagtcg attatggaga aagagataca gctatttata
     7861 atgcagaaat gatagcttct atgaatctag gcataccagt tatatatgct ggtaatgttg
     7921 aaaatcaaga ggaaataagg ttaatatttg aagatactaa ttataagctt tatataactg
     7981 aaaatgtata ccctaaaata gatttattga atatagaacc tacaagaaga atcattcaaa
     8041 gtgtatttga agaacacata actacagcac caggaatgaa atacattaaa gaaatggtta
     8101 atgaaaatat tactccaacc ccaggagctg taatggaagc ttctaaactt ttatataaaa
     8161 atattggaga tttattaact ctggatgtag gtggagcaac tactgatgta cattctgtta
     8221 cagatggaag tgactatatt aataaaattt tggttaatcc tgaacctact gcaaagagaa
     8281 gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa tatagtagaa gttataggta
     8341 aagaaaactt acaaagtgaa ctatctatag atattgatgc agtaatagag aattatcctc
     8401 caataccaaa atcaaaagaa gaaatattat ttgttgagag gttgacaaaa gaagctgttg
     8461 ttaaagctat gctaagacat tcaggaaaaa taagaaatat ctatggaaca actggaaaag
     8521 tcaaaattgc agaagggaaa gatttaacag aagtaagata tatagttggt acagggggag
     8581 cacttactag gctccctagt agaattgaaa tattagataa tatgctcaaa tacaataaaa
     8641 ataatgaatt actatttcca aaggaaaaaa caaagatatt aatagataat gactatatta
     8701 tggcatctat gggtgtttta tctaaaaaat atgaagaagc atctcttaag ctcctgttaa
     8761 aaagtttgaa ttttgaggag gaaagattat gtatcctagg ttagaaatag atatagaaaa
     8821 gttaaagcat aatgcaaaat taatttcgca gatgtgtcat gaaagaaata ttaagatatc
     8881 ttttgttact aaatcatttt gtgcacaaaa agaaatagta gaagaaatat gtagtgaagg
     8941 aatagaccat atagcagatt caagaataca aaacttaaaa aatttacaag atataaatct
     9001 acctaagata ttgattagaa tcccaatgtt aagtgaatta gaagaagtta tcaattattg
     9061 cgatattagt tttaactcag aattaggaac aataaagaaa ttaaatgaat tatgtgaaag
     9121 taaaaatatt attcataaga tagttcttat gtttgactta ggcgatttac gagaaggata
     9181 cttttatgaa gaagactttt ttagtaatgt aagagagata gtaagattaa aaaatattga
     9241 aataattggt atagctacaa acttaacctg ttatggagct attataccat ctagggaaaa
     9301 tataggcaga ctggtaagta tagcaaaaag aatggaagaa aaatttggaa taaatctaga
     9361 aatagtatct ggaggtaatt caagttctat tcatctgttg ataaataata atatgccaga
     9421 aggtattact aatttgagaa taggcgagtc cattctactt ggtagagaaa cggcatacgg
     9481 tgaaaatata aatggaacat atcaagatgc atttaaatta atatgccaag tagttgaatg
     9541 taaagaaaaa ccatcagttc ctattggaga aataggagtt gatgccttta gaaataaacc
     9601 tgtttatgaa gataagggaa tcttaaaaag ggcaatcata gctataggta aacaagatat
     9661 aaatattgac tctttaattc caatagacac tgatataaaa atattagggg caagttcaga
     9721 ccacatgata ctagatgtaa gtaacacaaa atatgattat aaattaggtg acaatttaga
     9781 atttttatta acttatgggg gtatcatgtc atctagtacg agcaaatatg tttcaaaaaa
     9841 aattatagtg tagtgtttaa ataattagga ggaaactatt ttatgaaaga gcgagaatgt
     9901 agaaaagcaa gtctgtttga tgcaatgata ccaattactt gtttgatatt atttttaagt
     9961 ctaggagtat tagtgtatgg ttcatcacct catgtaccac taataggagc agcagcagta
    10021 gcaggacttg tggctgtata tagacttgga tttaaatgga aagagttaga attaagtatg
    10081 tttaatagta taaagatggc aatgcaagca atcttaatca taattataat aggcgtactt
    10141 ataggaactt ggatagtaag tggagtagtt ccatgtatga tttactgggg actaaaaatt
    10201 ttatcaccaa atattttttt agtagcatca acattagtct gtgcaatagt atctcttgcg
    10261 acaggttcat cttggagcac aatgggtaca gtaggagtag ctcttttagg cattggtcaa
    10321 agtttaggta tgccaattgg attaatcgta ggttctataa tatctggagc ttactttgga
    10381 gataaactat cacctttatc tgaaacgact aacttagctc cagcaatggc aggaacagat
    10441 ttgtttacac atataaaata tatgctatat tcgactatac catcattatt aatttgttta
    10501 gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag cactagatat aaaacaaata
    10561 gaattaatta ggtctacatt agattctaca tttaatactt tatcaccagt attattatta
    10621 gctccagcaa tagtaattgg attggttgta tttaaagttc ctgctatacc agggcttata
    10681 gtaggtgctg tattgggagt attgtttgca gtagtttttc aaggagaatc tatgaatacg
    10741 atattagatg cagcacaaaa tggatatgtt tcgtcaacac aaatacagga ggtagatgct
    10801 ttattatcta aaggtgggat tatgaatatg atgtccactg tatccttaac aatatgcgct
    10861 ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg aagtagtagc atcttcttta
    10921 ctaagattag caaagggtgt atttggaaca gtattatgta ctatggtaac ttgtacaatt
    10981 accaatataa tagcaggtga acaatatcta tctatagtta taccaggaag aatgtataat
    11041 aaggaatata aaaagagagg tatacatcct aagatgttgt cgagagcttt agaagatagt
    11101 ggaactttaa cctctccatt agtaccatgg aatacatgcg gggcgtatat aacagcaaca
    11161 ttaggtgtat ctgcactaac atatggtcca tacgctttat taaatattat aaatccaata
    11221 gtatctttag tattaattgc atgtaagttt aaaatagcaa gaatagaaga tgaacctgaa
    11281 acttgtttaa attttgatgt ttgataatag ataacttatg taagaacgta ggaactaggg
    11341 gggtagcctg tggggaatta gtaagacata ttttagtgtg cgaaattcta taaatcagta
    11401 atcttgtgac tttagccatg ggaggttcag attagtaaat ttatagtttt atattagtct
    11461 atattttgat cttttaattg tttgtataaa ttttttatag aaaaagattt cttttagact
    11521 tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt ataagagcac tgtgtgacaa
    11581 gaatgatgag attatttttg ctatactttc gatataagaa atggaaggag gttatgtttt
    11641 atattcatat gaaataatta gttacacagt aaattttcgt ataatttggt ataaaaagat
    11701 tagaaatgaa aataaagtaa tggaggaata aaatgactag aatatccaat ataacaatag
    11761 ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac aattgacttt atggtagagt
    11821 tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa atatctggag gagagaggaa
    11881 ttttatcttc aggagcaccg attgtatgtt ttcataacat ggatttagca aaattggatg
    11941 ttgaagtagg gtttcctgta gccacttata tagatgggaa aaatgagatt ctacaaagga
    12001 ttgttcctgc acagaaaata ataactgcaa ttgatttagg accttatgaa ttacaagacc
    12061 ctactttaga agacttattt tcatgggcaa atagcaatgg atataaactt catggcgata
    12121 tatattacca gtatttaaat gatatcaatc gtccagctag tgaatatctt acgaaaatga
    12181 tgttacccat tatatagaaa gttaaagatt atcgtttgtt agttgataat tagaagaaca
    12241 tttactgcta ctacataaat tttatcctaa ataatatcaa ggtgatttaa tttatgtatt
    12301 ctaatgtaac cccaatatat tgttaaggtc tacttaagat gctcaaaagg ctatgcttaa
    12361 tttattgtta tataatttgt agagtggtat aatttagagg taatattata tagatatata
    12421 gggggaaaat atgaaaatat atgatatatt aacacgtttt tatattcaag atatagaaaa
    12481 agccatacct ttttatgaaa atcttttaaa agaaaaatgt tctttgcgtt tttcctataa
    12541 agaagttggg ttggaattag cgcaaatagg aaatgtttta ctattatctg gttctgatga
    12601 tgctcttaaa ccatttatag aaacaaaatc tacttttatg gttgattctg ttgatgaatg
    12661 gagaagttat ctattggata atggagcagt tgtagtaaga gataagaaaa aagttcctac
    12721 aggatataat atgacattga gacatccaga tggtacaatt atagaatatg ttcaacatac
    12781 aaagcaggat tagttaaaaa cagattttac tactgagcaa ctaattagaa tctattctta
    12841 gacgtacaat ttattatttt tggtattaac agattataag tcaaattata atatttcaaa
    12901 atagaataga aatttattta cataaaagag agtgtttaaa aatgaacttt ttagttcatg
    12961 aagcactctt ttttgtatga aatcaaatac atcaatatat aaaataaact gatgaagagc
    13021 ttaaattaaa ataaaaagat tcataacagg accccattaa actttaagat agggtaagtt
    13081 tgtaatcata ttaaataatt gtaaaaagct gtttttagtc gtataatata aatatttatc
    13141 atttaattaa ggaatataaa aggaatctaa taagaagaaa ataataaata actttttaga
    13201 tttactaaat gaaaagttat tgaaaattaa aggattagag tctttaatca gaaagtaaaa
    13261 agtaatatat taaatttatg aattgaaaac ttaaacaaca aatttgttta taaaatagca
    13321 agattcatta aatataacta agtaataatt gatataatgt tgcatggata aaaggaggga
    13381 agctgcaatg tcttacaaag taattaatgt cataactgct attgactata tagaggagca
    13441 tttatcagaa aagctggatt tagatatagt tgctaatgct gttcattact caaaatatca
    13501 cctgcataga actttcacaa cttctgttgg tctaacaatg catgattaca taaagcggag
    13561 aaagttgaca gaagcagcaa aattattggt attttcaaag aaaccaatta ttgaaattgc
    13621 attgatagct ggatatgaaa gtcaacaggc attcacttct atatttaaag ctatgtataa
    13681 aaaatctcca aataaatata ggaaagaaca agaattttat cctttgcagc taagatttgt
    13741 tttgaaaaaa gataacctat tttcaaagaa tgtactagag ttggaaaaag aaattaaact
    13801 tgcaagtatg tctgacatac cattatggat gaatttagtg agattagtca tagatggatt
    13861 tccaaatttg caagaggaag aatatatata tcaattagaa cagtatattt tagaagaaag
    13921 agctttaata ttaaaactta ataatgtcgc tattgcaaat atggtgttta atagagaaac
    13981 tagaagtatt gatttttttg gaatacatcc acagtatcgt aattcagaca ttgcacaagt
    14041 gtttttgaag aaagttattg aagatttctt gatagataca gatatttcaa ttactacctt
    14101 tcgagagggt gacaaagctg atacaggaca tagagatatg ataaagaagt tgggctttgc
    14161 agaagcagaa ttgcttgtgg aatttggata tcctactcaa aaatttattc ttccttgtgt
    14221 agaagaaaga gataatagac aaaattaatt gtagattaaa tgaaaagttt accacatatt
    14281 ttttgtttga attattttat atataagagc atttctgctt ttaatgtatt ttgcttgtaa
    14341 gtgtacttat ggtaataaat atagaaagat ttagtttttt aaaaaaggaa ctttaaagct
    14401 aaaaagctaa catttaagtt aaatagcaag aatgattaaa aactccaaaa cacaattaag
    14461 tataatgatt cagtgtaagt acttaattaa caaaggagga tttaaatgtc tgaaaataaa
    14521 caagtagtaa attgcgatat tattgcatct aattctaaac ataatatgtg tgaatatgat
    14581 gcagagttat taactttttt tccagatgag attgcacatc taatagagat aaataacaag
    14641 ctagacaatg cttttaagaa agctgaaaat cttgttgaca agcttgataa ggattatatg
    14701 gacgctaaaa tgtatatggt aaaaaatcgt ggtgaaatag acccacatga aatgtttcaa
    14761 aatgaacaag gactaaaaca aatagataac tatggggctt ttatggtaaa agttcgagat
    14821 aagattaata aaatcaaaga ttctccatac tttgctagaa tagattttag gctgaaagat
    14881 atggatgatg aatctaagta ttatattggt agatttgcat ttgattatga agatgagctt
    14941 ataatacttg actggcgttc tccaattgcc agtatgtttt atgattatga aatcggaaaa
    15001 gctggatatg atgctccaat aggatgggtg gatggagaaa taacaagaaa acgacaattt
    15061 aaaattaaaa atggaaaatt agaatatgca ctagaaagtt ccattaatat acaagatgat
    15121 attcttcaaa aagaacttag tcacacttca gatgaaaaaa tgaagtcaat catttcaaca
    15181 atacaaaagg aacaaaatca aattattaga aatgataaag ctgatacact gattattcaa
    15241 ggggtagctg gttctgggaa aacatctata gcattacaca gaattgcttt tcttctttat
    15301 cgatttaaag ataaaatatc agcaaataat gtaattatcc tatcacctaa taaagtcttt
    15361 ggagactata tttccaatgt ccttcctgag cttggagaag agccattatg tgaattaagt
    15421 tttgaaaata tagcagaggt acaattagat agagttatta attttgaaag tgagaaagac
    15481 ccattagaaa taaatgatgc taaatggtct gaaagagtgc gttttaaatc tacacttgat
    15541 tttgtaaaac ttattgatga ttatataaaa caaatgccaa ataagatttt tattccaaaa
    15601 gactacactt ttggaagttt tacagctaaa agtgactgga tacagagtag atttgaggca
    15661 tataacagat atccagttaa gaagagatta gagaaggtag ctgaagacat tcattataaa
    15721 tttgaatcag ataatattat ggaagaagat ttaccaaagg taaaaagtat acttaaaagt
    15781 ttgaatggaa tgttgacaat aaaaaatact cttacactat ataaggattt ctttaaacaa
    15841 atgaatatat caaacatgtt tgtgatggca acaaaaaaaa ctcttgaatg gtcagatgta
    15901 tacccattta tatatattca tgctgcatat gaaggtatac aagaagataa gattataaga
    15961 catgctgtta ttgatgaaat gcaggattac acacctattc aatatgctgt aataaactta
    16021 ctttttaagt gtaaaaagac tattcttgga gattttggac aattggttaa tcctaatcac
    16081 actcatacac tagatgatat gaaacagcta tataatgatg gggaactagt tatgttgaat
    16141 aagagttacc gttctacttt tgagattatt aatttcgcca aaaagattca agacatatct
    16201 tcattagagc ctattgaacg acatggtgaa gaaccagctt tattaaaatg taataacgag
    16261 caagatgaaa taaataagat aaaaatagaa attgaagaat ttaaaaagag tgataatgcg
    16321 actcttggta ttatattaaa gacagataat gacgctaaag ctgtatatga tgcgttaaga
    16381 gaaaaatata gtgttcactt aatatcttct gaaagttcta gctttacaaa aggagtatcc
    16441 ataacttcta ttaaaatgtc aaaaggttta gagtttgatg aagttattgt accttcagta
    16501 aataataaaa catattatag tgattatgac cgcagtttgt tatatattgc ttgtacacga
    16561 gctatgcata aacttaagtt gacatatact ggtgaattga ctcaacttat agatatgtag
    16621 attatatact tattatgtaa gtgtttactt atataaatat aacctgtcaa taaaattcat
    16681 taataaatca ataaaaacaa tatttgtaaa agtaaaagct ataaattata tattaaattt
    16741 tataatttat agcttttata atactgctta tcttaatcct cccaatagca aaataatttt
    16801 aaacatgcca tattttataa atgtaataac aattatacaa ttaaaataac tttcccaaac
    16861 taacttgaca tttaaaactt actattgtaa tatttaaata ttacaatagt aagttttaaa
    16921 tgtggaatca attattatat cacaaattaa aaggaggaca ggatgttatt aatagttact
    16981 taattatctt cgcaataggt ttaatcaaca taattgtact aggtgaagaa aatataagag
    17041 atacagctat tcatttagtc ttgtttctta taagtagaac ctagattgga gtatattaca
    17101 aataatgtct actattttaa tagtaggtta taaatttaaa tatgggagat gatataaatt
    17161 atgaaaagaa aaaagaattt tatttggata gctatcttat tggttggtgt agttatggta
    17221 atgtattatg tagggaaaaa acataacgat ataaatcaaa aaactgataa gaattatata
    17281 aaatctgaat taaataagtc tgaattaaat aaatccaaaa ttgaaaacaa taataaaaac
    17341 aaaaaagaaa aaattaatat ggttgactat agtgattgtt ttgaaggtat tagtggagga
    17401 gctatttttt ataatactaa aaataaagaa tataatatat ataacaaaga attaattgaa
    17461 acaagaaggt caccatgttc tacttttaaa atagtttcaa ctttaattgg tttagaaaaa
    17521 ggggtaataa actcaaaaga atcagttatg ggatatgatg gtacagagta tccaaataaa
    17581 aattggaata aaaatcttag tttagaagag gcatttaagg agtcttgtgt ttggtactac
    17641 aaaaaattga tagataaagt tgatgctaaa tctgtccaaa acattttaga tgatttaaag
    17701 tatgggaatt gcgatattag cgaatgggaa ggagatttaa aaaatggaaa gggacattta
    17761 aatggatttt ggttagaatc ttcattacaa atatctccaa aagaacaagt gcaaacaatg
    17821 gcaaaaatat ttgagggaga tacaaacttt aaaaaagagc atattaatat tttaagagat
    17881 attatgaaaa tagatgtaaa tgataaaaac ataaatgtat atggaaaaac tggaacagga
    17941 tttgatgaaa aaaataaacg tgtggatgca tggtttgttg gtatgctaga gcgtgaaggt
    18001 gacacctatt attttgctat taaatcagat gattccaata aagaaattac agggccaaag
    18061 gtaaaggaaa ttgcaattaa tataattaag aaatattata gtgtaaggga ataaacttaa
    18121 ttttatttgt aaatattatt tacctatagt ctgtaatatt tattaaataa ttttactaaa
    18181 ttgagtctat atagagcaaa actcctttag tacataaaat acttgctatt gattattaaa
    18241 ttgaatacct atagatttaa agttaatac
//
LOCUS       DAMJZE010000077        17742 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJZE010000077 DAMJZE010000000
VERSION     DAMJZE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.077"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            484..1344
                     /locus_tag="PC541_002160"
     CDS             484..1344
                     /locus_tag="PC541_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2839625.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1334..3205
                     /locus_tag="PC541_002161"
     CDS             1334..3205
                     /locus_tag="PC541_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2839626.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3225..4679
                     /locus_tag="PC541_002162"
     CDS             3225..4679
                     /locus_tag="PC541_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2839627.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5704..6912
                     /locus_tag="PC541_002163"
     CDS             5704..6912
                     /locus_tag="PC541_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839628.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6943..8322
                     /locus_tag="PC541_002164"
     CDS             6943..8322
                     /locus_tag="PC541_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839629.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8428..9618
                     /locus_tag="PC541_002165"
     CDS             8428..9618
                     /locus_tag="PC541_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2839630.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9746..11074)
                     /locus_tag="PC541_002166"
     CDS             complement(9746..11074)
                     /locus_tag="PC541_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839631.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11692..12906)
                     /locus_tag="PC541_002167"
     CDS             complement(11692..12906)
                     /locus_tag="PC541_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2839632.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            13332..14048
                     /locus_tag="PC541_002168"
     CDS             13332..14048
                     /locus_tag="PC541_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2839633.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14183..14809
                     /locus_tag="PC541_002169"
     CDS             14183..14809
                     /locus_tag="PC541_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2839634.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(15009..16553)
                     /locus_tag="PC541_002170"
     CDS             complement(15009..16553)
                     /locus_tag="PC541_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2839635.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(16579..17220)
                     /locus_tag="PC541_002171"
     CDS             complement(16579..17220)
                     /locus_tag="PC541_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2839636.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            17490..17681
                     /locus_tag="PC541_002172"
                     /pseudo
     CDS             17490..17681
                     /locus_tag="PC541_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         6559 a         2085 c         2927 g         6171 t
ORIGIN      
        1 aaatgaaact aaatttgaag ctttacctat taatatatct taatagtgat taaataaaac
       61 taaacttaag actttatatg ttaatatatc ttaagaattt atttgttaaa atacagttca
      121 ttatatttaa aagcattact atgataattc ataatagtaa aatagataat ggttcagaca
      181 atattataaa tactagtgac ggtaaaatag tatcaattta gtatataaac cttgtcagat
      241 aatcgcataa cagagcttgt agttatagaa taaaaaaata tctaggaaac cgttgacaga
      301 tttatatgga tagtataaca tatagaatat gaaattaaac aaaaaagaat tttaattatg
      361 gcgacgtagt cataattttg agtatgttac tgattagcag gcagaactca agtatatatg
      421 tgaaaaatag agggatactc tatacatata tgcttgagtt ctttttattg aagggaggaa
      481 tatatgaaaa taattcagat tttaaataat aatgtagcct tagtaaaaag aggtaaaaat
      541 gaaattattg tatttgttaa aggaattggc tttaaaaaaa gagtgggaca agtaataaat
      601 gaaaatgaaa tagagagaac atatatacta gattcatacg atatgctaga acactttagt
      661 tatctactac aaaactcaga ctccaataat atagttctta ttaatgaaat aattgaatat
      721 ggtgagaaaa ctttaaatat aaaggcaagt gattatctaa gcttaacctt actagaccat
      781 ttagagtttt tacttaaaag atgtaaaaag aatcaattta taaaaagtcc tttggtatgg
      841 aatgttaaaa gattttatcc aaagcatttt gaaattggaa tgcatgcact aaaattaatt
      901 ggtgatagta agggaattaa tcttcctgat gatgaggcag tatcaatagc acttcatttt
      961 gtaaatatgg aggtaaataa agagtctcat gatagtacaa ttgtagagtt gagaacttta
     1021 gcagatatag tttctataat aaaatatcat tttaatgtag agctagatga aacttcaaca
     1081 aattatatga gatttactac tcatctacag tactttatcc aaagaattat gtcagaggaa
     1141 gcttcaatag atgaagataa ttctatagat ttatataaac aagtaagtca actttatcca
     1201 aagtcatttg gaagtgttca aaagataaga aaatatataa aatcacaatt taatgtcgat
     1261 atatctataa gtgaagaaac ttatttaatg ttgcatataa atagggtgac tcaaagactg
     1321 gaggcaaaat cagatgaaat ataaagattt aaatgaaaat ataataaaac ttataggggg
     1381 aaaagaaaat ataaagtcgg tagcgcattg tgttactcgt ttaagattta ctttaaatga
     1441 taggagtatc gctcaaacag aagaaattaa aaaattaaaa ggagtaattg atgtagtttc
     1501 taatgatgtt gcttatcaaa ttataatagg tactcatgtt gtagatgtat ataacgagtt
     1561 tatgtctatg ataggtttaa gttcaagtga atcaagtata aaaagtgaaa gaaaaaaggg
     1621 aattagaggt atcaaaggta tatttaattc catctttgtg gtagtctcag agactatgac
     1681 ttcagtaatt gaagtactac ttgcagctgg tatacttgca agtatattag cacttttaaa
     1741 tatgtctggc atacttcaga gtgacagtcc tacatatcaa atattagata cattaagaag
     1801 tgctacattt cacttcttac cagtattaat tgcaatagca tcagcaaaaa gacttaatgt
     1861 aaatccatat atagcagtag tattagcagt tacgttatta tcttcatcaa tagatggtgt
     1921 agaaggatta agtttatttg gtataaattt acagtctaca acttatgcaa acagttttat
     1981 tccaatactt ttaggagtat gggcaatggg aattataatt gaaggtttga aaaaaatatt
     2041 acctaaagca ctacactatt ttttagttcc agtattatct ttaataataa ctctatcagt
     2101 tacattaact gtatttggac ctattggtat gtggattggt gatggattaa actatgcatg
     2161 tatgttttta attgatacat tgggtaattg gagtgttgtt gcactgtatg cagctttaca
     2221 accactctta atagtctttg gtgcagcaaa ctttactatg cctatatctt taaattctgt
     2281 gacaacattg gggtatgacc caatttttat gggcgctgca acaatatctg atattgcagt
     2341 ttgtggtgca atgtttggat atttcttaaa aactaggaat aaagaacaaa aacaattatt
     2401 tggtacagta agttttagtg cacttatggg agttacagaa ccttcagtgt ttggtgtgtt
     2461 tatgaaatat agaagaccat ttgtagcagt aatgataggt ggaggtatag gtggtttaat
     2521 agcaggttta gctcaagtta aaacatatgg aatggtttgg ggattagctg cattaccaac
     2581 gtatcttgtt ggaggagagg tatctaactt tacatttatg ataataagtg tagtagtatc
     2641 atttgtagca gcaacagcag cttcctattt actaggaata ccttcagatg aaaaagaaga
     2701 agttaagggt gataatgaaa aagaattaga actaataatc aacgagaaca attcaagaac
     2761 tattagtatt ggaaatgttg caaaaggtca ggtaattgca cttggggaag ttaatgacaa
     2821 agcattttcc tctggagcat taggtaaagg ggttggagtt atacctagtg aatcaaaatc
     2881 aactgttata tctcctgtag atggaactat aactatagta tttccaacta aacatgcata
     2941 tggtatagtg actgatgaag gattagaaat attaattcat attgggatag atactgtaaa
     3001 cttagatgga aaattctttg aaagtcttgt aacacaaaat caaagggtta aaaaaggaga
     3061 tgctttagca gtatttgaat ctgataaaat tatagaggaa ggctttgatt ctactataat
     3121 aactgtagtt acaaatacta gcgattattt agatgtaatc tcatcaaatg atgaatcaga
     3181 agaattatta actgtaatag tataaatttt aggagggaaa attcatgaat attaaagata
     3241 catttttctg gggaggaagt attgcagccc atcaatgtga aggttcatgg gatagtgaca
     3301 acaaaggacc tgcaattatg gattttgtaa ctaagggctc ttatgaaatg ccaagagtaa
     3361 ttactgataa aatagaagaa aaattggatt atccatctca taatggaata gatttttata
     3421 atagatataa agaagatata gctttattta aagaaatggg atttagtgca ttaagaatat
     3481 ctatagattg gtcaagaatt tttccaaatg gagatgatga aaatcctaat gaattaggga
     3541 ttaaatacta tgaaggtctg atagatacat taatagaaaa taatatagaa cctattgtaa
     3601 cactatatca ttttgaatta cctatgaatt tagtacataa atatggttca tggaataaca
     3661 gaaaacttat agatttgtat ttaaagtaca gtgagactgt aataagaaga tttgatgata
     3721 aggtaaagta ctgggttaca tttaatgaga tgaaccatat tgaccctcaa acagagcact
     3781 cggatatttt tacttatatg atagcaggta ttaagtatag tgaaatagaa aataaggttc
     3841 aaacattggc aaatgtaggg tataatatga ctttggcggg tgtaaaggtt gcaaagttgg
     3901 ctagagaaat caatcccaac aataaagttg gatgtgtatt tggactaaat ccaatttatc
     3961 catacaattg taatcctagt aatgtgttaa atgcattttt agataatgac cgtgattatt
     4021 atcaaattga tgcaatgtgc aatgggaagt ttccacaata taaattaaaa gaatatgaaa
     4081 aaaataatat aactattgaa gtgactgatg aagacaaaaa agcatttaaa gaaggtaagt
     4141 tagattttat aggacttaat tattattttt caagtgtatc tactccaaaa gaaaatttat
     4201 caggtgataa atctttattt ggtgggattc aaaatccata tttggaacaa agtaagtggg
     4261 ggtgggcaat agaccctata ggtttaagat ttaccttgaa ctatatttac agaaagtatc
     4321 aattacctat cttaattaca gaaaatggtt taggggcata tgataaaatc gaagaagatg
     4381 gaactataaa tgatgattat agaattgaat atttaggtaa gcatctatct caaatgaagt
     4441 ctgcaattga agaagataat gttgaatgtt ttggatattt gatgtgggga ccaattgatt
     4501 tagtcagtgc aactacaggt cagatgagta agagatatgg atttatctat gttgacttag
     4561 atgatgaagg caaaggaagt tttaagagat ataagaaaaa atcatttgat tggtacaaag
     4621 aagtaataaa atctaatgga gaagttttag atttttctac ggatatagta gtagggtagg
     4681 aataagataa atagaagtta ggaactaatg ttaattagtg tctagtaaag catttaaaat
     4741 agagtatagg gtctttaaag taaagtgtgt attcttctta aaagtaatgg caactaaaat
     4801 tactataaaa aggagaatac acatttttta tgtttatact taagtaaata tttagacata
     4861 ttcatagtat aatttactca ttatataaaa atagtatgat ttactcacta tataacttta
     4921 taaattttct ggaaaaatgt cagaatatat tgacaaagaa aagatttaaa attataatct
     4981 atattaagaa tagtttgaca atataaaaac aagtattttt ataaatagtg aaattatttt
     5041 tattgtcaat ttaagaataa tttaggacta ctataaagat aaaggtatag atgtcattat
     5101 atgataaatt atacttgtat agaaagaatt ttaaagtatg gcttatcatt gatttaatat
     5161 aataaataat ttttgtttct agtaatagtt cctaggaata agatatctaa attcgtttag
     5221 aaatagtcta atttacaaag gaggggatgc actaatagat aaaatttcta tataaagaag
     5281 acttaaagtg aagtatattc tgataatatt ggtttaatgg gtatattatt atttcgtttt
     5341 agatatttta agaataattt aagacgttta taaaaatgta ttctatatct aatatttaaa
     5401 taaatataag tcttccaaca tgtatactaa tatagagagt agttgaagta caattctaac
     5461 tcgtaaaatt atgtagaaag taaaaatcaa aaagaaatct agtaagttga taactatagt
     5521 taatatttag agagttaact ctagttatat ttatttagat attttaggaa taatttagga
     5581 cgtatataat atcatcaaaa aatatcaagc tataccatat gtagttaaga ataactgcat
     5641 aatatacatt ttataattat tgttttattg ataaaaaatt attatattta ggaggaaaca
     5701 acaatggaaa atttttggaa tccacttact tgctttactg tatgtgtaat gatttatgct
     5761 tttagtgagt ttttatctaa gaaaacaaaa ggtgcaattt caacattgtt atttgtatgt
     5821 attatatttt tactaggatt ttggacaaat attttaccaa aagacattac aggagcatca
     5881 gggatattag ctgcaatgag caactttggt atagctttat taataactaa cttaggaaca
     5941 cttataaatc ttgaagactt atgtcatgaa tggaaaacgg ttgtaattgc attagtaggt
     6001 attgtaggaa taggagttgg ctgtttaaca gtgggtagta tgttatttgg cagagagtat
     6061 gctttaatag cagcacctcc aatagcaggt tctactgttg ctgggattat cgtaacaact
     6121 gttgcagaag ctgcaaatag acctgaactt gctgcatttg taattctagt attatcattt
     6181 caaaagttct ttggtattcc gataactaca tattgtataa ataaagaatt aaaatttaaa
     6241 agagataacg gggatttttt gaaagatgag tcaaaaagta attttaaact tccaagtatg
     6301 agaattttta aagaagattt aggtaaaaaa tcatctactc tagtgtatct tgcaaagctt
     6361 tcatttgttg ctatgattgg taattttgta ggattggcta ctttaatacc aggatttacc
     6421 cctgcaaact attatttaaa tcctaatata gcatgtttgt tagtagggtt gatttttaca
     6481 cgaataggtt ttttagaaaa aggaatttta gataaatctc aatctaatgg aattataatg
     6541 tttggttgta tgttgatgtt acctggagga cttgctaagg taactccaac agcattgttt
     6601 gggatgattg ttccaactat aggaatactt gttgtaggtt ctgtatttat aatattattt
     6661 actgcaattg taggaaaact attgggatat tctccaagaa tgtcagctgc aattggtgta
     6721 acttgtatgc tagcttatcc tgcaacacag ataatttcca acgaaggtat tgatgctatg
     6781 gaaggaactg aagaagaaaa acaaagggca acagattatt tattaccaaa gatgattata
     6841 ggaggatttg ttactgtaac cattgcatca gtggcatttg ctagtataat aggtccaatt
     6901 atatttggtt gatagtaaat atacttatag gaggaatagg aaatgatagg aagtatatct
     6961 ataaatgaga tagataaaaa aagattcaaa cttgatgatt taagtaagaa aatatgggaa
     7021 aatccagaaa aagcatttaa agaatttaaa gcatgtgaaa atacagcaaa ctttctaaga
     7081 agtgaaggat ttgatgtgga agtaggtgta ggaggtttag ccacggctat tagagctagt
     7141 tttggttcag gtaaaccagt tataggtttt atgggagaat ttgatgcatt acctggttta
     7201 aatcaaaaag tttcaacaaa gcaagaagca tttgaattgg gtgcatatgg tcatggatgt
     7261 gggcataatt tattatgtac agcacatgta ggggcagtag tagggcttaa aagagagatg
     7321 atagaaaata acttaagtgg gactatagta ttttatgcat gtccaggaga agaacaatta
     7381 acaggtaaag gatttatggc tagaggtgga gcgtttgaag ggttagactt agctataaat
     7441 tttcatccaa ataaaattag tgaagctaca attggtactt caacagctgt taactctgta
     7501 aagtttcact ttaagggaaa gactgcacat gcagggtcag accctcaaaa tggaagaagt
     7561 gcactagatg ctgtggaact tactaatgtg ggagctaatt atttgagaga acatgttact
     7621 tctgatgtaa ggattcatta tacaattaca gatggaggag tagcacccaa catagttcct
     7681 gataaagcat cagtatggta ttatacaaga gctttaagta gagaagctgt agaaagtaca
     7741 tatgaaagac ttgtaaaggt agcaaaagga gctgcaatga tgactgaaac tgaagttgaa
     7801 gtagagtttt taggagggtg ttataatact ttaaacaatc atgttttagc aaatcttgta
     7861 tcagaatgta tgaatgagat taaacaagag ccttggactc gagaggaatt ggattttgca
     7921 gctgaattag atagacaaag cccacaacaa tataaggcta tgataagtaa atataatttg
     7981 ccagaaggaa ctcatctata ttatggtgga ggaaatgtta caaactttaa tagttatggc
     8041 tctactgata ttggagatgt aatgcatata gttccgacag catacttttt tacagggtgt
     8101 acgaatctag gagcaccagg tcatagctgg cagtttacat cttgtgcagg aagttctata
     8161 ggagaaaaag gtatgatata tgcatcaaag attatggcta tgtttggagc taaaatatta
     8221 aataatccag agatagctaa aaaggcaaaa gaagaatttg ataaatctat gaatggtaaa
     8281 acatataaat gtcctattcc agatgatatt ccaactcctt aaaaagacat gaaatttaat
     8341 atataaaatt ataattttta tatcagcatc atttcattac taaaagaaag tgagattttg
     8401 ctgactatct gattaggagg aaggaatatg aaaaattttt ttaaagaagc gcagtcaata
     8461 gaaggggaaa ttattagatg gagaaggtat ttacatgaaa atcccgaaat tggttgcaat
     8521 ctaccgaaaa cagtaacctt tgttaccagt actttagaaa aaatgggata ttctccgaaa
     8581 ttgacgtcta ataatggaat agtagctgtt ttagacagtg aaaatggtgg aaagacctta
     8641 ttattaaggg cagatatgga tgcacttcct attagggagg agacggattt accttttaaa
     8701 agtaagaata aagatgcatc tcatagttgt ggacacgata atcacacagc aatgctctta
     8761 ggagcagcta aaatacttat ggataataag cacttactta aaggtagagt aaagtttgta
     8821 tttgaaccag atgaagagac taccagaggt gcaaaatcaa tgattgatga tggtatatta
     8881 gaaaatcctc aggtcgatgc agctatggca tttcacagta tggtgggtaa atttcttaac
     8941 acaggtcaag tagcttattc acgtggtcca gctatggcat ctgcggatat atttgaaata
     9001 acagtagaag gaaaagcaac tcatggagca agccctgaat ttggagtgga cccaataaac
     9061 ataatgtcgc atatacaaat agcattaaac actatacttt caagagaaaa acctcagaaa
     9121 gagcctgtag ttttaactat tggtatgttt aatgcaggaa acgtaccaaa tgctatacca
     9181 aataaagcat ctatgtcagg aactttaaga acatttgacc aatttgttag agaaaaatta
     9241 aaaaatagaa ttattgaaat atctacagga atagctaaat catttggagg aaatgctaaa
     9301 gtggagttta aaatggggac tcctgcagtt ataaatgatt tggatgtagg agatgagatt
     9361 gtatcttatg ttaaagaagt tataggaaat gaaaatgtaa tagactttcc atctgcaatg
     9421 ggttcagaag atttttcaga ggttttacta agggttccag gagtattttt atggattgga
     9481 atgggaagta ttagtcaggg atataatttt ggtatgcata atcctaaagc aatttttaat
     9541 gaagagggat tagtttatgg ttcagcttta tttgctcatt gtgcagtaaa atggttagaa
     9601 aataatttta gtagataata acataaaata ttattaaata tcataaaagg tatatcttaa
     9661 aaagatgcaa agatatacct tttagaaagt catatataat tttacttaat attagatgat
     9721 aaaatatttt tatgcatttt ggattttaat atttattttc tgatataaat aatttgtata
     9781 ccctttcagg tctgcctttt gttgagtttt gtttttcata taatatagta gcgtttccag
     9841 actttaagag actacttaga aatctattag cagttctaac tgttacattt aaacatgaag
     9901 ataattcctt aggagtaact tcatctcttt gtagaatttc agtaacagat ataatctttt
     9961 gaatagtaag agtagacaat ttagaacttt tggcaattgt atttatatgt ggagttactt
    10021 tatttgaaac agtaagtaac ttatcacaca gtaaaggtcc tattgtctca taattttcgt
    10081 taactaagaa actgtaattt aaaggattta ttttagattc cttattagca cttatggcac
    10141 tagacatagc ttgctttata tcattaccaa ttccatatcc aatacatact ttaatatttg
    10201 tactattttc aatagattct tttatataac atgcagtaaa atttttagtt aatgatttaa
    10261 tcgtttcaca tttagtgaaa attttaaaag aattactatt tttttcaatt ataaaatcgg
    10321 ttaatttatc ttttttaata ctatttaata ttttataaac ttggtctaga gtagagtcat
    10381 cataatttgt atctttaact gttatatata ttacacttgg ttgatttccc ttcattctat
    10441 gtaaatgaat atcttttatt aagttattaa aagtattttt taataaatct atatcagggt
    10501 atatgaaatg aaagtttact cctgcatttt gtaacatagg aacaatacta ctaaaacgag
    10561 tagtagaaaa atcgattttc ccttcattcc atagattaat atgttttttt gcaatctttt
    10621 tttcaagttt tgctatgtca gataaactca ttttagatgc attttcatca atactatcca
    10681 ttaagtctcc aaatgtgcca tttgataaat aatatgatat atcttttgga ttatctgccc
    10741 agtctgttaa gtcaaagtat gctctggaaa aatctaatgt attatattta aagataagct
    10801 ttagaaacat atcatagtaa ctttgtaagt cagtaccaaa atcaactaga ggttttttaa
    10861 tagttccagc cttttttgta atagcttttt tggggatggg gccactaact gcaaatccat
    10921 caaacctatc ttctatttct aaatataaat ctccaacatg agaaaaatct ctatagatat
    10981 atatttcaat ttctgcatta atatttaact tttttataga attatctaca aactctttta
    11041 aaaattcggt tgttatgact gctattttca tcatagatta cttccttttc aattattata
    11101 attattttat attaaatatt acattaatct attgttctag ttaaaatcgt atttcaatta
    11161 aattcaatta aaataaataa ctctttataa aaagcaatta tttaggtaga ggaatatttg
    11221 ctaactttaa tagtctttgt taactttaat tttatttaag ttattatagt cttactaatt
    11281 ttataattgt agatttaact actttatgtt cttttattgt ttatgtagtt gatttagaag
    11341 ttattttaac catttatttt aattgaagtt gtttgacttt taaatccttt gtgtaatctt
    11401 tgtttttcta gccatgataa aagtatttct atttgtatca ctatatataa taatatcatg
    11461 acaaaatatt tattgtctaa ctaattataa tctatccaaa atgaaaatgg gtgtgagata
    11521 ttattacaac aaattgtatt agtattaaaa agattaaaaa attaaaaaac taaacagtta
    11581 aataattaaa cgattaagtg actgtcatat aacaatttat aattttattt aacagccact
    11641 taggtattta agagcgatag aattgagtgt atatcaaatt ataataattt cctagtaaat
    11701 aattgaaaaa taaggtagtc catttttata tttgatatct ggtttacctt gaataagtgg
    11761 atatagataa tttagggctt catcagttat ataattacat tcttcattta tccactcttt
    11821 aggaaaatat tttgtcttgt tagctatttt agaaatatca gtatgactaa cttcatactt
    11881 ataaggatta ttgcatgttc tattaatgat agccataact cctgtatatc cttttttaga
    11941 tagttctatc ccatatgcac caacttcagc tgcttgtttt aaatcagtat ctgaagctaa
    12001 atgcatagca catctttgta ttacaccaag ttctaaggtt tttactcttg ttgtaattga
    12061 tgatgatact ataagattct ttagatagtt gcttactcca cctaattggg aatgaccaaa
    12121 tttatcatga gcagaagttt tttgttcgca tataaagttg ccgtatttat cccttatacc
    12181 ttctgatgca actatataag ccttattttt ttctttaaat atttttctaa catcttctat
    12241 aaacttatct tcatagaaat caatttctgg aaggtatatt aaatcaacaa gtggagtatc
    12301 attaagtcta gctagtgaag aagaagctgc taaccatcca gtatctctac ccatagtttc
    12361 tactataaat ataccattat ttttgtatat agaaaaatct aagtatgttt ccattattag
    12421 tgtagaaata agctttgcag cagaaccaaa tccaggacag ttgtcagttc ctaataaatc
    12481 attatctatt gttttaggaa tacctataaa ttttatttta caagtagtgt ttttgcagta
    12541 ttgagaaaat ttcatcactg tgtccataga atcatttccg cctatataaa ataatgtatc
    12601 tattttatat tcatctagta tatttataac tctatcataa tcataagaat ttattttata
    12661 atcagccagt ttgtatctac aagaaccaag accagacgaa ggtgtttgct ttaaatttaa
    12721 tacattatct ttgtttaagg agtctgaatc tattatattt ttgtttaata taccttcaat
    12781 cccattgata ccaaaatata ctttatcaaa gtatttagtt tctaaatttt tttcataaat
    12841 accaactgca gaagcattga taacagctgt agggcctcca gattgggcaa taatacagtt
    12901 tctcatttta catctccctt tcattaatat tgttatgaat ggtacttttt atcgagtaaa
    12961 tttatattta tagtctgatt atatattaaa tgaactagct atatcaattg agttagtata
    13021 gacaataaat gccataagaa tgtggtaaaa aactaacttt ttatatacaa ttaatgtcta
    13081 taatttacca caatataaag gtacttttaa ggagtagtca aagtgttgtg aaaaaaaata
    13141 tatcagtgta atattaattt acaaagcaaa ataagttcac agttaacaaa agcagaataa
    13201 gttcataaaa gaaatatata cagcaataaa agtaatgcta aataaaagta atactggatt
    13261 aaaaaatatt aaaaaaatat taaaaactaa tatttaatac taattaaagt aaaatatgga
    13321 ggtatacaaa tatgaaacac ataaaaatag cagcaggatt agcacatgta aattacggtc
    13381 atatatcagc cgtagttaaa gaagcaatgg atgcaggtgt agactacgta cattcagatg
    13441 cagcagatat gcatgactta aaaaatatgc aacttatggg tggtcatcaa ataatagaag
    13501 caataagacc ggtgacagat aaaccaatag aatgccatat atacacaaga gattgtgata
    13561 gattatttat agaaaaaata gcagcagcag gatgtaactt gttgataata ccagcagaac
    13621 attttttagg tgctcctctt gcatacataa taaattactg tagagaattt ggtatgaaag
    13681 taggattaac aattggatgt tacacaccac tatgttttgt tgatgaagct atatatgata
    13741 tagatagatt gcaaatagtc gttcatggtg ttgatgaaac tgatggaaaa gataactggg
    13801 gatggagaaa gagtgctgtt gatttagtaa aaagagctag aaaaatgata gacgaaaaga
    13861 atcctaagtg tgaattagca atagatggtg gactaagagc agacaatatg gagccattaa
    13921 tagaatgtaa tccagacgtt gtaatattat cttcagcaat attcaaggat aaagatggta
    13981 taacagcagg tgttaagaat tgtagaaaag ctatagatga agcagctaca aagtttggtt
    14041 tagaataaga taataaacta tgataaaaga gatgctttct taatgtttta aggaagcatt
    14101 aattttatga aaaactacag gaagctagac taatttaatt gattttttat actgaaacaa
    14161 tataatcata ggaggttatt aaatgaaaaa agaaatgata aaagattatt tactgataac
    14221 aatatcagca tttttaatgg cattagcagt taattttttc tttgcagaac atacattagc
    14281 tccaggaggg ataacaggat tatcagtcgt acttagcaca ttcttatcaa tgcctgttga
    14341 gaatatatca ttaggaatat ctatgccact tttagtaatg ggattattat ttttagggaa
    14401 aggttttgga ataaaaacat tatatataac tcttatgggc ccattattct taaaagtaat
    14461 accacaaact cacataactg acaatctatt agtagcagga gtgataggtg ggttattggt
    14521 aggaactgct atagggatag caataataag gggctgtagt actggtggaa ctgatttagt
    14581 tgcaatgctt attaataagg taattaagtt tctgaagttg ccagttatat tatttatgct
    14641 agatggattt ataatagtag cgtctggaat aataagtaag aattttatga tatctatata
    14701 ttcattgata tctttattac tcataataaa aacaatagga tttgtaacta caaagtttgg
    14761 taagaatact gaagaaatta ataatataaa taatgcttgt gctcaataaa aataataaaa
    14821 tttactttaa atataaaagt ttaaaaaata taaaagctta aataaaaaga ttaaatataa
    14881 aaaatgaagg tgtctcaaaa tgaatttttt agttcatgag gcactttttc tatgaaatca
    14941 aatatatttt tatcatttgg acaatgaaaa ataggtttat ctctatgttg agacaagcct
    15001 atttttgttt atttataaga taatatttta tttttttcag aatatagttt ttttataatt
    15061 gtactaagtc tagaaatatc attcttactt aaaagagcat ttatatttat cagttcagca
    15121 ttatgttcaa agtctacagg agcagtggtt agtattaagt ccacatcatc aaagttcgta
    15181 aatgaaatag aagatagagg tatagattta actatattaa tctgattaaa ttcaaaactt
    15241 aacttttgga ctaataaatt agatacacca tcactacagt gacatattaa aatagtattt
    15301 aggggagatt tgcttctatc tattgctgat attaaatata aagcaagata tcctatttcg
    15361 tcatcattga cttcataatt catcaactgt ttgataacag tattagactc tttagcagcc
    15421 ttataagtgt aaggaaatgt tctctttata tcatttaata tgggatttgt aatagtaaac
    15481 ccatagttta ttctgtgtag aactgattta aggtgattag caagagactt tattaatatt
    15541 aaatcatcct ttaagtttaa atttagtata cttccccaat gaacagctat tgcttcagat
    15601 agattatact ctgtaatttc ttcattttta agaggttcaa gagtagtagt tttctttatt
    15661 gatttaagta atccttctat atgaagaagg atataacaga cctcttcatc aataaattct
    15721 acgtcaaaag cttcacttag gttagcacat aaaatttttg atttttcaaa gtattcatgt
    15781 tctaataatt ctctaaaaaa accttcagat aagttaatat atcttttaat tgaaattctt
    15841 ttaatacata tagctatatg tattaagaag tttatataat actcatcact aaggttactt
    15901 ttttctactt gtaaggtgtg tagagtgatt tgagataatt tctcaaaatc atagttaaat
    15961 agctccttaa atatttttgt tgatggggta tctttgcatt cattagattt cgagaataat
    16021 atttctttaa gttcagtata acttttatta accgtagcaa gttcaaaaat tgcttttcga
    16081 atatctttct cttttccctc taaatatact ccattattgg tttttctaac tagagtcact
    16141 ttaaagtttt gcaaaaaatt ttgaataact accaagtctt tatgcacagt agctctacta
    16201 acatgtagtt cattgcaaat atcacgaatc ctaaacttag aatttttaat aaataattta
    16261 cttagaatat acctttttct atcttcagga gagtatgcat atatacaatt tgatttatca
    16321 ttgatatctc taataacttt aagttttata tcttcattac ctagaataat aacccctgaa
    16381 ccagtttttt tatctaggga taaattaaac tctaaaagcc attcatctaa aataacaagg
    16441 tcattacgaa tagttttatt actaactttt aattcttcgg caatagtatc tacagtaata
    16501 tagtctgagc tgttgagtaa taattcagca atatctaata atcttttgtt caatgcaaac
    16561 cctcctaaat cttagttact atttattaac cttggaccaa tctctttcaa aagtattggc
    16621 accatctatt gtaagctggt taaaagaagc ttttttaaat aaatcatatg ttacagctgc
    16681 ataatcacat ccacaaagag atgcttgagt taagtggaat acatttttaa tagaagctgc
    16741 aacaacttta gtattatatc catttattga tataaggtta catatttctg taaggttttt
    16801 tataccatca ttcccatttt catcatttct acctataaaa gggaataggt aatcagcacc
    16861 tgcatttata gctaatgatg cttgatttac attgaaaatc aaggtacatg cagtttttac
    16921 acctttacta tttaatatgt ttataagttc taagccaact tcagagaaat ttatttttat
    16981 tatagtgttt ttatttatat ttaatatctt atcacatgaa ttaattaatt cgtataaatt
    17041 atttccaatg acttcagcag ttaatatttc tggatttaaa ttagcacatg tctcaataaa
    17101 ttcatggaag tttactttct ctttaagata catagatgga ttagcggtta ttcccttaaa
    17161 tccatacttt agacactttt gaatttcatc aagattagca ctgtctatca aatatttcat
    17221 aatttaattc tcctttaagc tatttgtcat aaaaaagtgt atatactcta tttttattat
    17281 taagtcaatt atacaataag attgattata aaattataac atgggtactt aataaaattt
    17341 acataatgat atggtaataa taaaacattg attgtctaat aattaccata agctttagta
    17401 aataagagtt aacagtgaag gtattgcctt aaaaatccta tgatatgtat aatttgaata
    17461 tgaaggcaat aatttaaggg aggaaaaata tgattaactt ttatcaacca tcaagaataa
    17521 attttggaca aggaacttta agtgaattac ctagaataat aggaaaatat ggaaagaaat
    17581 gtttgttagt tactactcca aatgtagagc cattagacaa gttatataaa agagttacat
    17641 tgaatttaat agaaaaaggt atagaagttg tacactttga ttaaaaaata gctacgcaca
    17701 ctcgtgactt tagtcatgag ttaatagcta aaatagtcag ca
//
LOCUS       DAMJZE010000078        17719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJZE010000078 DAMJZE010000000
VERSION     DAMJZE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.078"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            102..656
                     /gene="tcdR"
                     /locus_tag="PC541_002173"
     CDS             102..656
                     /gene="tcdR"
                     /locus_tag="PC541_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2839637.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            974..8074
                     /gene="tcdB"
                     /locus_tag="PC541_002174"
                     /gene_synonym="toxB"
     CDS             974..8074
                     /gene="tcdB"
                     /locus_tag="PC541_002174"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2839638.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8196..8696
                     /gene="tcdE"
                     /locus_tag="PC541_002175"
     CDS             8196..8696
                     /gene="tcdE"
                     /locus_tag="PC541_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2839639.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8775..8936
                     /locus_tag="PC541_002176"
     CDS             8775..8936
                     /locus_tag="PC541_002176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839640.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9001..9207
                     /locus_tag="PC541_002177"
     CDS             9001..9207
                     /locus_tag="PC541_002177"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839641.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            9424..17556
                     /gene="tcdA"
                     /locus_tag="PC541_002178"
                     /gene_synonym="toxA"
     CDS             9424..17556
                     /gene="tcdA"
                     /locus_tag="PC541_002178"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2839642.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         7071 a         1893 c         2797 g         5958 t
ORIGIN      
        1 aatttatttg ccgattatat aattataatg actgatttaa ttccaatgtt gtcaaaattt
       61 tcaaataaat catcattata aatataagag aggatgattt tatgcaaaag tctttttatg
      121 aattaattgt tttagcaaga aataactcag tagatgattt gcaagaaatt ttatttatgt
      181 ttaagccatt agtaaaaaaa cttagtagag ttttacatta tgaagaggga gaaacagatt
      241 taataatatt ttttattgaa ttaataaaaa atattaaatt aagtagcttt tcagaaaaaa
      301 gcgatgctat tatagtcaaa tatattcata aatcattact gaataagact tttgagttgt
      361 ctagaagata ttctaaaatg aagtttaatt ttgtagaatt tgatgaaaat atcttaaata
      421 tgaaaaataa ttatcaaagt aagtctattt ttgaggaaga tatttgtttt ttcggatata
      481 ttttgaaaga attatctggt attcaaagaa aagttatttt ttataaatat ttaaaaggat
      541 attctgatag agaaatatca gtgaaattaa aaatatctag acaagctgtt aataaggcta
      601 aaaatagagc atttaaaaaa ataaaaaaag actatgaaaa ttattttaac ttgtaattaa
      661 tgagtttaaa gaaatattta caatagaaat caaattttat aattaacttt attgtaaaat
      721 taataactta atctaagaat atcttaattt ttatatttta tatagaacaa tgtttacata
      781 tttatttcag acaacgtctt tattcaatcg aagagcaaat taatcaactg agtgtcttaa
      841 atttaaaatg ttaggaagtg aatgtatatg aaaacctaag tagatattag tatattttat
      901 aaatagaaag gaggatatat aaaagagttt tagcatttag atgtaaaaat attatagtaa
      961 aggagaaaat tttatgagtt tagttaatag aaaacagtta gaaaaaatgg caaatgtaag
     1021 atttcgtact caagaagatg aatatgttgc aatattggat gctttagaag aatatcataa
     1081 tatgtcagag aatactgtag tcgaaaaata tttaaaatta aaagatataa atagtttaac
     1141 agatatttat atagatacat ataaaaaatc tggtagaaat aaagccttaa aaaaatttaa
     1201 ggaatatcta gttacagaag tattagagct aaagaataat aatttaactc cagttgagaa
     1261 aaatttacat tttgtttgga ttggaggtca aataaatgac actgctatta attatataaa
     1321 tcaatggaaa gatgtaaata gtgattataa tgttaatgtt ttttatgata gtaatgcatt
     1381 tttgataaac acattgaaaa aaactgtagt agaatcagca ataaatgata cacttgaatc
     1441 atttagagaa aacttaaatg accctagatt tgactataat aaattcttca gaaaacgtat
     1501 ggaaataatt tatgataaac agaaaaattt cataaactac tataaagctc aaagagaaga
     1561 aaatcctgaa cttataattg atgatattgt aaagacatat ctttcaaatg agtattcaaa
     1621 ggagatagat gaacttaata cctatattga agaatcctta aataaaatta cacagaatag
     1681 tggaaatgat gttagaaact ttgaagaatt taaaaatgga gagtcattca acttatatga
     1741 acaagagttg gtagaaaggt ggaatttagc tgctgcttct gacatattaa gaatatctgc
     1801 attaaaagaa attggtggta tgtatttaga tgttgatatg ttaccaggaa tacaaccaga
     1861 cttatttgag tctatagaga aacctagttc agtaacagtg gatttttggg aaatgacaaa
     1921 gttagaagct ataatgaaat acaaagaata tataccagaa tatacctcag aacattttga
     1981 catgttagac gaagaagttc aaagtagttt tgaatctgtt ctagcttcta agtcagataa
     2041 atcagaaata ttctcatcac ttggtgatat ggaggcatca ccactagaag ttaaaattgc
     2101 atttaatagt aagggtatta taaatcaagg gctaatttct gtgaaagact catattgtag
     2161 caatttaata gtaaaacaaa tcgagaatag atataaaata ttgaataata gtttaaatcc
     2221 agctattagc gaggataatg attttaatac tacaacgaat acctttattg atagtataat
     2281 ggctgaagct aatgcagata atggtagatt tatgatggaa ctaggaaagt atttaagagt
     2341 tggtttcttc ccagatgtta aaactactat taacttaagt ggccctgaag catatgcggc
     2401 agcttatcaa gatttattaa tgtttaaaga aggcagtatg aatatccatt tgatagaagc
     2461 tgatttaaga aactttgaaa tctctaaaac taatatttct caatcaactg aacaagaaat
     2521 ggctagctta tggtcatttg acgatgcaag agctaaagct caatttgaag aatataaaag
     2581 gaattatttt gaaggttctc ttggtgaaga tgataatctt gatttttctc aaaatatagt
     2641 agttgacaag gagtatcttt tagaaaaaat atcttcatta gcaagaagtt cagagagagg
     2701 atatatacac tatattgttc agttacaagg agataaaatt agttatgaag cagcatgtaa
     2761 cttatttgca aagactcctt atgatagtgt actatttcag aaaaatatag aagattcaga
     2821 aattgcatat tattataatc ctggagatgg tgaaatacaa gaaatagaca agtataaaat
     2881 tccaagtata atttctgata gacctaagat taaattaaca tttattggtc atggtaaaga
     2941 tgaatttaat actgatatat ttgcaggttt tgatgtagat tcattatcca cagaaataga
     3001 agcagcaata gatttagcta aagaggatat ttctcctaag tcaatagaaa taaatttatt
     3061 aggatgtaat atgtttagct actctatcaa cgtagaggag acttatcctg gaaaattatt
     3121 acttaaagtt aaagataaaa tatcagaatt aatgccatct ataagtcaag actctattat
     3181 agtaagtgca aatcaatatg aagttagaat aaatagtgaa ggaagaagag aattattgga
     3241 tcattctggt gaatggataa ataaagaaga aagtattata aaggatattt catcaaaaga
     3301 atatatatca tttaatccta aagaaaataa aattacagta aaatctaaaa atttacctga
     3361 gctatctaca ttattacaag aaattagaaa taattctaat tcaagtgata ttgaactaga
     3421 agaaaaagta atgttaacag aatgtgagat aaatgttatt tcaaatatag atacgcaaat
     3481 tgttgaggaa aggattgaag aagctaagaa tttaacttct gactctatta attatataaa
     3541 agatgaattt aaactaatag aatctatttc tgatgcacta tgtgacttaa aacaacagaa
     3601 tgaattagaa gattctcatt ttatatcttt tgaggacata tcagagactg atgagggatt
     3661 tagtataaga tttattaata aagaaactgg agaatctata tttgtagaaa ctgaaaaaac
     3721 aatattctct gaatatgcta atcatataac tgaagagatt tctaagataa aaggtactat
     3781 atttgatact gtaaatggta agttagtaaa aaaagtaaat ttagatacta cacacgaagt
     3841 aaatacttta aatgctgcat tttttataca atcattaata gaatataata gttctaaaga
     3901 atctcttagt aatttaagtg tagcaatgaa agtccaagtt tacgctcaat tatttagtac
     3961 tggtttaaat actattacag atgcagccaa agttgttgaa ttagtatcaa ctgcattaga
     4021 tgaaactata gacttacttc ctacattatc tgaaggatta cctataattg caactattat
     4081 agatggtgta agtttaggtg cagcaatcaa agagctaagt gaaacgagtg acccattatt
     4141 aagacaagaa atagaagcta agataggtat aatggcagta aatttaacaa cagctacaac
     4201 tgcaatcatt acttcatctt tgggggtagc tagtggattt agtatacttt tagttccttt
     4261 agcaggaatt tcagcaggta taccaagctt agtaaacaat gaacttgtac ttcgagataa
     4321 ggcaacaaag gttgtagatt attttaaaca tgtttcatta gttgaaactg aaggagtatt
     4381 tactttatta gatgataaaa taatgatgcc acaagatgat ttagtgatat cagaaataga
     4441 ttttaataat aattcaatag ttttaggtaa atgtgaaatc tggagaatgg aaggtggttc
     4501 aggtcatact gtaactgatg atatagatca cttcttttca gcaccatcaa taacatatag
     4561 agagccacac ttatctatat atgacgtatt ggaagtacaa aaagaagaac ttgatttgtc
     4621 aaaagattta atggtattac ctaatgctcc aaatagagta tttgcttggg aaacaggatg
     4681 gacaccaggt ttaagaagct tagaaaatga tggcacaaaa ctgttagacc gtataagaga
     4741 taactatgaa ggtgagtttt attggagata ttttgctttt atagctgatg ctttaataac
     4801 aacattaaaa ccaagatatg aagatactaa tataagaata aatttagata gtaatactag
     4861 aagttttata gttccaataa taactacaga atatataaga gaaaaattat catattcttt
     4921 ctatggttca ggaggaactt atgcattgtc tctttctcaa tataatatgg gtataaatat
     4981 agaattaagt gaaagtgatg tttggattat agatgttgat aatgttgtga gagatgtaac
     5041 tatagaatct gataaaatta aaaaaggtga tttaatagaa ggtattttat ctacactaag
     5101 tattgaagag aataaaatta tcttaaatag ccatgagatt aatttttctg gtgaggtaaa
     5161 tggaagtaat ggatttgttt ctttaacatt ttcaatttta gaaggaataa atgcaattat
     5221 agaagttgat ttattatcta aatcatataa attacttatt tctggcgaat taaaaatatt
     5281 gatgttaaat tcaaatcata ttcaacagaa aatagattat ataggattca atagcgaatt
     5341 acagaaaaat ataccatata gctttgtaga tagtgaagga aaagagaatg gttttattaa
     5401 tggttcaaca aaagaaggtt tatttgtatc tgaattacct gatgtagttc ttataagtaa
     5461 ggtttatatg gatgatagta agccttcatt tggatattat agtaataatt tgaaagatgt
     5521 caaagttata actaaagata atgttaatat attaacaggt tattatctta aggatgatat
     5581 aaaaatctct ctttctttga ctctacaaga tgaaaaaact ataaagttaa atagtgtgca
     5641 tttagatgaa agtggagtag ctgagatttt gaagttcatg aatagaaaag gtaatacaaa
     5701 tacttcagat tctttaatga gctttttaga aagtatgaat ataaaaagta ttttcgttaa
     5761 tttcttacaa tctaatatta agtttatatt agatgctaat tttataataa gtggtactac
     5821 ttctattggc caatttgagt ttatttgtga tgaaaatgat aatatacaac catatttcat
     5881 taagtttaat acactagaaa ctaattatac tttatatgta ggaaatagac aaaatatgat
     5941 agtggaacca aattatgatt tagatgattc tggagatata tcttcaactg ttatcaattt
     6001 ctctcaaaag tatctttatg gaatagacag ttgtgttaat aaagttgtaa tttcaccaaa
     6061 tatttataca gatgaaataa atataacgcc tgtatatgaa acaaataata cttatccaga
     6121 agttattgta ttagatgcaa attatataaa tgaaaaaata aatgttaata tcaatgatct
     6181 atctatacga tatgtatgga gtaatgatgg taatgatttt attcttatgt caactagtga
     6241 agaaaataag gtgtcacaag ttaaaataag attcgttaat gtttttaaag ataagacttt
     6301 ggcaaataag ctatctttta actttagtga taaacaagat gtacctgtaa gtgaaataat
     6361 cttatcattt acaccttcat attatgagga tggattgatt ggctatgatt tgggtctagt
     6421 ttctttatat aatgagaaat tttatattaa taactttgga atgatggtat ctggattaat
     6481 atatattaat gattcattat attattttaa accaccagta aataatttga taactggatt
     6541 tgtgactgta ggcgatgata aatactactt taatccaatt aatggtggag ctgcttcaat
     6601 tggagagaca ataattgatg acaaaaatta ttatttcaac caaagtggag tgttacaaac
     6661 aggtgtattt agtacagaag atggatttaa atattttgcc ccagctaata cacttgatga
     6721 aaacctagaa ggagaagcaa ttgattttac tggaaaatta attattgacg aaaatattta
     6781 ttattttgat gataattata gaggagctgt agaatggaaa gaattagatg gtgaaatgca
     6841 ctattttagc ccagaaacag gtaaagcttt taaaggtcta aatcaaatag gtgattataa
     6901 atactatttc aattctgatg gagttatgca aaaaggattt gttagtataa atgataataa
     6961 acactatttt gatgattctg gtgttatgaa agtaggttac actgaaatag atggcaagca
     7021 tttctacttt gctgaaaacg gagaaatgca aataggagta tttaatacag aagatggatt
     7081 taaatatttt gctcatcata atgaagattt aggaaatgaa gaaggtgaag aaatctcata
     7141 ttctggtata ttaaatttca ataataaaat ttactatttt gatgattcat ttacagctgt
     7201 agttggatgg aaagatttag aggatggttc aaagtattat tttgatgaag atacagcaga
     7261 agcatatata ggtttgtcat taataaatga tggtcaatat tattttaatg atgatggaat
     7321 tatgcaagtt ggatttgtca ctataaatga taaagtcttc tacttctctg actctggaat
     7381 tatagaatct ggagtacaaa acatagatga caattatttc tatatagatg ataatggtat
     7441 agttcaaatt ggtgtatttg atacttcaga tggatataaa tattttgcac ctgctaatac
     7501 tgtaaatgat aatatttacg gacaagcagt tgaatatagt ggtttagtta gagttgggga
     7561 agatgtatat tattttggag aaacatatac aattgagact ggatggatat atgatatgga
     7621 aaatgaaagt gataaatatt atttcaatcc agaaactaaa aaagcatgca aaggtattaa
     7681 tttaattgat gatataaaat attattttga tgagaagggc ataatgagaa cgggtcttat
     7741 atcatttgaa aataataatt attactttaa tgagaatggt gaaatgcaat ttggttatat
     7801 aaatatagaa gataagatgt tctattttgg tgaagatggt gtcatgcaga ttggagtatt
     7861 taatacacca gatggattta aatactttgc acatcaaaat actttggatg agaattttga
     7921 gggagaatca ataaactata ctggttggtt agatttagat gaaaagagat attattttac
     7981 agatgaatat attgcagcaa ctggttcagt tattattgat ggtgaggagt attattttga
     8041 tcctgataca gctcaattag tgattagtga atagataaaa atatgttaaa tatatcctct
     8101 tatacttaaa tatataaaaa taaacaaaat gatacactac ataaagtgtt ctatctaata
     8161 tgaagattta ccaataaaaa ggtggactat gatgaatgca cagtagttca cctttttata
     8221 tttctaatgg taacaaaata tttttttata taaacctagg aggcgttatg aatatgacaa
     8281 tatctttttt atcagagcat atatttataa agttagtaat tttaactata tcatttgata
     8341 cattattagg atgtttaagt gcaataaaaa gtcgtaaatt taattctagt tttggaatag
     8401 atggaggaat cagaaaagta gcaatgatag catgtatatt ttttttatca gtagttgaca
     8461 ttcttacaaa gtttaacttt ttatttatgt taccacaaga ttgtatcaat tttttaagac
     8521 taaaacatct tggaatatct gaatttttct ctattttatt tattttatat gaaagtgtaa
     8581 gtatattaaa aaatatgtgc ttatgtggat taccagtacc taagagatta aaggaaaaaa
     8641 tagcaatttt actagatgca atgacagatg aaatgaatgc taaggatgaa aagtaagtaa
     8701 tggtagatat aataaagata ttaacaaata aaaagtgtta tccaaataag aatagctgaa
     8761 agttatcata attcatgaaa ctaataatga aaacgaggga gcagatgcca agagacacac
     8821 aagtattaaa tacatataat ttcgaagcaa gtgttcatta ctatatagat gacaaggtag
     8881 tatatcaaac attggttcac aaagatggtg catggtcagt tggtaaaatc tattaagcta
     8941 cattagttac agatatcaca aactataata gttaaacata gaaatatgtg taaattgtga
     9001 ttgaaattat tcaaaaacac aaaaatacgt gatgaaggac aaaatgatat agaaaataag
     9061 tatcaaacct taataaatga tttaattgat agtttaaaag ttataggaaa aatatataaa
     9121 gaaataaaaa cattaaaaaa atataagata tgtttacaaa ttactatcag acaatctcct
     9181 tatctaatag aagagtcaat taactaattg agtatcttta aattgaaatg ttaggaagtg
     9241 atttaaatat gaaaacttaa attataaaaa atcaatatta atttattttt aaaaaataga
     9301 aaggagtgta taagatttat tttcaaagtt taaaaacaag aaaatcaatt taaatttcag
     9361 aaggaataaa tgtggttata gaagtggatt tattatcaaa aataataata ctaggaggtt
     9421 tttatgtctt taatatctaa agaagagtta ataaaactcg catatagcat tagaccaaga
     9481 gaaaatgagt ataaaactat actaactaat ttagacgaat ataataagtt aactacaaac
     9541 aataatgaaa ataaatattt acaattaaaa aaactaaatg aatcaattga tgtttttatg
     9601 aataaatata aaacttcaag cagaaataga gcactctcta atctaaaaaa agatatatta
     9661 aaagaagtaa ttcttattaa aaattccaat acaagccctg tagaaaaaaa tttacatttt
     9721 gtatggatag gtggagaagt cagtgatatt gctcttgaat acataaaaca atgggctgat
     9781 attaatgcag aatataatat taaactgtgg tatgatagtg aagcattctt agtaaataca
     9841 ctaaaaaagg ctatagttga atcttctacc actgaagcat tacagctact agaggaagag
     9901 attcaaaatc ctcaatttga taatatgaaa ttttacaaaa aaaggatgga atttatatat
     9961 gatagacaaa aaaggtttat aaattattat aaatctcaaa tcaataaacc tacagtacct
    10021 acaatagatg atattataaa gtctcatcta gtatctgaat ataatagaga tgaaactgta
    10081 ttagaatcat atagaacaaa ttctttgaga aaaataaata gtaatcatgg gatagatatc
    10141 agggctaata gtttgtttac agaacaagag ttattaaata tttatagtca ggagttgtta
    10201 aatcgtggaa atttagctgc agcatctgac atagtaagat tattagccct aaaaaatttt
    10261 ggcggagtat atttagatgt tgatatgctt ccaggtattc actctgattt atttaaaaca
    10321 atatctagac ctagctctat tggactagac cgttgggaaa tgataaaatt agaggctatt
    10381 atgaagtata aaaaatatat aaataattat acatcagaaa actttgataa acttgatcaa
    10441 caattaaaag ataattttaa actcattata gaaagtaaaa gtgaaaaatc tgagatattt
    10501 tctaaattag aaaatttaaa tgtatctgat cttgaaatta aaatagcttt cgctttaggc
    10561 agtgttataa atcaagcctt gatatcaaaa caaggttcat atcttactaa cctagtaata
    10621 gaacaagtaa aaaatagata tcaattttta aaccaacacc ttaacccagc catagagtct
    10681 gataataact tcacagatac tactaaaatt tttcatgatt cattatttaa ttcagctacc
    10741 gcagaaaact ctatgttttt aacaaaaata gcaccatact tacaagtagg ttttatgcca
    10801 gaagctcgct ccacaataag tttaagtggt ccaggagctt atgcgtcagc ttactatgat
    10861 ttcataaatt tacaagaaaa tactatagaa aaaactttaa aagcatcaga tttaatagaa
    10921 tttaaattcc cagaaaataa tctatctcaa ttgacagaac aagaaataaa tagtctatgg
    10981 agctttgatc aagcaagtgc aaaatatcaa tttgagaaat atgtaagaga ttatactggt
    11041 ggatctcttt ctgaagacaa tggggtagac tttaataaaa atactgccct cgacaaaaac
    11101 tatttattaa ataataaaat tccatcaaac aatgtagaag aagctggaag taaaaattat
    11161 gttcattata tcatacagtt acaaggagat gatataagtt atgaagcaac atgcaattta
    11221 ttttctaaaa atcctaaaaa tagtattatt atacaacgaa atatgaatga aagtgcaaaa
    11281 agctactttt taagtgatga tggagaatct attttagaat taaataaata taggatacct
    11341 gaaagattaa aaaataagga aaaagtaaaa gtaaccttta ttggacatgg taaagatgaa
    11401 ttcaacacaa gcgaatttgc tagattaagt gtagattcac tttccaatga gataagttca
    11461 tttttagata ccataaaatt agatatatca cctaaaaatg tagaagtaaa cttacttgga
    11521 tgtaatatgt ttagttatga ttttaatgtt gaagaaactt atcctgggaa gttgctatta
    11581 agtattatgg acaaaattac ttccacttta cctgatgtaa ataaaaattc tattactata
    11641 ggagcaaatc aatatgaagt aagaattaat agtgagggaa gaaaagaact tctggctcac
    11701 tcaggtaaat ggataaataa agaagaagct attatgagcg atttatctag taaagaatac
    11761 attttttttg attctataga taataagcta aaagcaaagt ccaagaatat tccaggatta
    11821 gcatcaatat cagaagatat aaaaacatta ttacttgatg caagtgttag tcctgataca
    11881 aaatttattt taaataatct taagcttaat attgaatctt ctattggtga ttacatttat
    11941 tatgaaaaat tagagcctgt taaaaatata attcacaatt ctatagatga tttaatagat
    12001 gagttcaatc tacttgaaaa tgtatctgat gaattatatg aattaaaaaa attaaataat
    12061 ctagatgaga agtatttaat atcttttgaa gatatctcaa aaaataattc aacttactct
    12121 gtaagattta ttaacaaaag taatggtgag tcagtttatg tagaaacaga aaaagaaatt
    12181 ttttcaaaat atagcgaaca tattacaaaa gaaataagta ctataaagaa tagtataatt
    12241 acagatgtta atggtaattt attggataat atacagttag atcatacttc tcaagttaat
    12301 acattaaacg cagcattctt tattcaatca ttaatagatt atagtagcaa taaagatgta
    12361 ctgaatgatt taagtacctc agttaaggtt caactttatg ctcaactatt tagtacaggt
    12421 ttaaatacta tatatgactc tatccaatta gtaaatttaa tatcaaatgc agtaaatgat
    12481 actataaatg tactacctac aataacagag gggataccta ttgtatctac tatattagac
    12541 ggaataaact taggtgcagc aattaaggaa ttactagacg aacatgaccc attactaaaa
    12601 aaagaattag aagctaaggt gggtgtttta gcaataaata tgtcattatc tatagctgca
    12661 actgtagctt caattgttgg aataggtgct gaagttacta ttttcttatt acctatagct
    12721 ggtatatctg caggaatacc ttcattagtt aataatgaat taatattgca tgataaggca
    12781 acttcagtgg taaactattt taatcatttg tctgaatcta aaaaatatgg ccctcttaaa
    12841 acagaagatg ataaaatttt agttcctatt gatgatttag taatatcaga aatagatttt
    12901 aataataatt cgataaaact aggaacatgt aatatattag caatggaggg gggatcagga
    12961 cacacagtga ctggtaatat agatcacttt ttctcatctc catctataag ttctcatatt
    13021 ccttcattat caatttattc tgcaataggt atagaaacag aaaatctaga tttttcaaaa
    13081 aaaataatga tgttacctaa tgctccttca agagtgtttt ggtgggaaac tggagcagtt
    13141 ccaggtttaa gatcattgga aaatgacgga actagattac ttgattcaat aagagattta
    13201 tacccaggta aattttactg gagattctat gcttttttcg attatgcaat aactacatta
    13261 aaaccagttt atgaagacac taatattaaa attaaactag ataaagatac tagaaacttc
    13321 ataatgccaa ctataactac taacgaaatt agaaacaaat tatcttattc atttgatgga
    13381 gcaggaggaa cttactcttt attattatct tcatatccaa tatcaacgaa tataaattta
    13441 tctaaagatg atttatggat atttaatatt gataatgaag taagagaaat atctatagaa
    13501 aatggtacta ttaaaaaagg aaagttaata aaagatgttt taagtaaaat tgatataaat
    13561 aaaaataaac ttattatagg caatcaaaca atagattttt caggcgatat agataataaa
    13621 gatagatata tattcttgac ttgtgagtta gatgataaaa ttagtttaat aatagaaata
    13681 aatcttgttg caaaatctta tagtttgtta ttgtctgggg ataaaaatta tttgatatcc
    13741 aatttatcta atactattga gaaaatcaat actttaggcc tagatagtaa aaatatagcg
    13801 tacaattaca ctgatgaatc taataataaa tattttggag ctatatctaa aacaagtcaa
    13861 aaaagcataa tacattataa aaaagacagt aaaaatatat tagaatttta taatgacagt
    13921 acattagaat ttaacagtaa agattttatt gctgaagata taaatgtatt tatgaaagat
    13981 gatattaata ctataacagg aaaatactat gttgataata atactgataa aagtatagat
    14041 ttctctattt ctttagttag taaaaatcaa gtaaaagtaa atggattata tttaaatgaa
    14101 tccgtatact catcttacct tgattttgtg aaaaattcag atggacacca taatacttct
    14161 aattttatga atttattttt ggacaatata agtttctgga aattgtttgg gtttgaaaat
    14221 ataaattttg taatcgataa atactttacc cttgttggta aaactaatct tggatatgta
    14281 gaatttattt gtgacaataa taaaaatata gatatatatt ttggtgaatg gaaaacatcg
    14341 tcatctaaaa gcactatatt tagcggaaat ggtagaaatg ttgtagtaga gcctatatat
    14401 aatcctgata cgggtgaaga tatatctact tcactagatt tttcctatga acctctctat
    14461 ggaatagata gatatatcaa taaagtattg atagcacctg atttatatac aagtttaata
    14521 aatattaata ccaattatta ttcaaatgag tactaccctg agattatagt tcttaaccca
    14581 aatacattcc acaaaaaagt aaatataaat ttagatagtt cttcttttga gtataaatgg
    14641 tctacagaag gaagtgactt tattttagtt agatacttag aagaaagtaa taaaaaaata
    14701 ttacaaaaaa taagaatcaa aggtatctta tctaatactc aatcatttaa taaaatgagt
    14761 atagatttta aagatattaa aaaactatca ttaggatata taatgagtaa ttttaaatca
    14821 tttaattctg aaaatgaatt agatagagat catttaggat ttaaaataat agataataaa
    14881 acttattact atgatgaaga tagtaaatta gttaaaggat taatcaatat aaataattca
    14941 ttattctatt ttgatcctat agaatttaac ttagtaactg gatggcaaac tatcaatggt
    15001 aaaaaatatt attttgatat aaatactgga gcagctttaa ctagttataa aattattaat
    15061 ggtaaacact tttattttaa taatgatggt gtgatgcagt tgggagtatt taaaggacct
    15121 gatggatttg aatattttgc acctgccaat actcaaaata ataacataga aggtcaggct
    15181 atagtttatc aaagtaaatt cttaactttg aatggcaaaa aatattattt tgataatgac
    15241 tcaaaagcag tcactggatg gagaattatt aacaatgaga aatattactt taatcctaat
    15301 aatgctattg ctgcagtcgg attgcaagta attgacaata ataagtatta tttcaatcct
    15361 gacactgcta tcatctcaaa aggttggcag actgttaatg gtagtagata ctactttgat
    15421 actgataccg ctattgcctt taatggttat aaaactattg atggtaaaca cttttatttt
    15481 gatagtgatt gtgtagtgaa aataggtgtg tttagtacct ctaatggatt tgaatatttt
    15541 gcacctgcta atacttataa taataacata gaaggtcagg ctatagttta tcaaagtaaa
    15601 ttcttaactt tgaatggtaa aaaatattac tttgataata actcaaaagc agttaccgga
    15661 tggcaaacta ttgatagtaa aaaatattac tttaatacta acactgctga agcagctact
    15721 ggatggcaaa ctattgatgg taaaaaatat tactttaata ctaacactgc tgaagcagct
    15781 actggatggc aaactattga tggtaaaaaa tattacttta atactaacac tgctatagct
    15841 tcaactggtt atacaattat taatggtaaa catttttatt ttaatactga tggtattatg
    15901 cagataggag tgtttaaagg acctaatgga tttgaatatt ttgcacctgc taatacggat
    15961 gctaacaaca tagaaggtca agctatactt taccaaaatg aattcttaac tttgaatggt
    16021 aaaaaatatt actttggtag tgactcaaaa gcagttactg gatggagaat tattaacaat
    16081 aagaaatatt actttaatcc taataatgct attgctgcaa ttcatctatg cactataaat
    16141 aatgacaagt attactttag ttatgatgga attcttcaaa atggatatat tactattgaa
    16201 agaaataatt tctattttga tgctaataat gaatctaaaa tggtaacagg agtatttaaa
    16261 ggacctaatg gatttgagta ttttgcacct gctaatactc acaataataa catagaaggt
    16321 caggctatag tttaccagaa caaattctta actttgaatg gcaaaaaata ttattttgat
    16381 aatgactcaa aagcagttac tggatggcaa accattgatg gtaaaaaata ttactttaat
    16441 cttaacactg ctgaagcagc tactggatgg caaactattg atggtaaaaa atattacttt
    16501 aatcttaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa aaaatattac
    16561 tttaatacta acactttcat agcctcaact ggttatacaa gtattaatgg taaacatttt
    16621 tattttaata ctgatggtat tatgcagata ggagtgttta aaggacctaa tggatttgaa
    16681 tactttgcac ctgctaatac tcataataat aacatagaag gtcaagctat actttaccaa
    16741 aataaattct taactttgaa tggtaaaaaa tattactttg gtagtgactc aaaagcagtt
    16801 accggattgc gaactattga tggtaaaaaa tattacttta atactaacac tgctgttgca
    16861 gttactggat ggcaaactat taatggtaaa aaatactact ttaatactaa cacttctata
    16921 gcttcaactg gttatacaat tattagtggt aaacattttt attttaatac tgatggtatt
    16981 atgcagatag gagtgtttaa aggacctgat ggatttgaat actttgcacc tgctaataca
    17041 gatgctaaca atatagaagg tcaagctata cgttatcaaa atagattcct atatttacat
    17101 gacaatatat attattttgg taataattca aaagcggcta ctggttgggt aactattgat
    17161 ggtaatagat attacttcga gcctaataca gctatgggtg cgaatggtta taaaactatt
    17221 gataataaaa atttttactt tagaaatggt ttacctcaga taggagtgtt taaagggtct
    17281 aatggatttg aatactttgc acctgctaat acggatgcta acaatataga aggtcaagct
    17341 atacgttatc aaaatagatt cctacattta cttggaaaaa tatattactt tggtaataat
    17401 tcaaaagcag ttactggatg gcaaactatt aatggtaaag tatattactt tatgcctgat
    17461 actgctatgg ctgcagctgg tggacttttc gagattgatg gtgttatata tttctttggt
    17521 gttgatggag taaaagcccc tgggatatat ggctaaaata tatgtttgat aaaaaattat
    17581 tcctgtgcta ctaagaaatt atttttatat aataaatatt gagatttaat taaaagtcat
    17641 gtgttattgt aatacatgac ttttagttaa aatttttcta tcatttaata atctattatt
    17701 cttgactatt ttataataa
//
LOCUS       DAMJZE010000079        17517 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJZE010000079 DAMJZE010000000
VERSION     DAMJZE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.079"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(208..435)
                     /gene="acpP"
                     /locus_tag="PC541_002179"
     CDS             complement(208..435)
                     /gene="acpP"
                     /locus_tag="PC541_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2839643.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            607..1032
                     /locus_tag="PC541_002180"
     CDS             607..1032
                     /locus_tag="PC541_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839644.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            1080..1286
                     /locus_tag="PC541_002181"
     CDS             1080..1286
                     /locus_tag="PC541_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839645.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            1468..1647
                     /locus_tag="PC541_002182"
     CDS             1468..1647
                     /locus_tag="PC541_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839646.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            1681..1881
                     /locus_tag="PC541_002183"
     CDS             1681..1881
                     /locus_tag="PC541_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839647.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            2134..3150
                     /gene="ccpA"
                     /locus_tag="PC541_002184"
     CDS             2134..3150
                     /gene="ccpA"
                     /locus_tag="PC541_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2839648.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(3273..3671)
                     /locus_tag="PC541_002185"
     CDS             complement(3273..3671)
                     /locus_tag="PC541_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839649.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            3883..4794
                     /locus_tag="PC541_002186"
     CDS             3883..4794
                     /locus_tag="PC541_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839650.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            4909..5089
                     /gene="ssrS"
                     /locus_tag="PC541_002187"
     ncRNA           4909..5089
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC541_002187"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5272..6489
                     /gene="cdeC"
                     /locus_tag="PC541_002188"
     CDS             5272..6489
                     /gene="cdeC"
                     /locus_tag="PC541_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2839651.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(6590..7912)
                     /locus_tag="PC541_002189"
     CDS             complement(6590..7912)
                     /locus_tag="PC541_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2839652.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(7963..8757)
                     /locus_tag="PC541_002190"
     CDS             complement(7963..8757)
                     /locus_tag="PC541_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2839653.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            8867..10312
                     /locus_tag="PC541_002191"
     CDS             8867..10312
                     /locus_tag="PC541_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2839654.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            10341..10550
                     /locus_tag="PC541_002192"
     CDS             10341..10550
                     /locus_tag="PC541_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2839655.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            10570..11964
                     /locus_tag="PC541_002193"
     CDS             10570..11964
                     /locus_tag="PC541_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2839656.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            12204..12956
                     /locus_tag="PC541_002194"
     CDS             12204..12956
                     /locus_tag="PC541_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2839657.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            12982..13395
                     /locus_tag="PC541_002195"
     CDS             12982..13395
                     /locus_tag="PC541_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2839658.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            13399..14142
                     /locus_tag="PC541_002196"
     CDS             13399..14142
                     /locus_tag="PC541_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2839659.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            14142..14612
                     /locus_tag="PC541_002197"
     CDS             14142..14612
                     /locus_tag="PC541_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2839660.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            14629..15387
                     /locus_tag="PC541_002198"
     CDS             14629..15387
                     /locus_tag="PC541_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2839661.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            15371..16213
                     /locus_tag="PC541_002199"
     CDS             15371..16213
                     /locus_tag="PC541_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2839662.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(16373..17278)
                     /locus_tag="PC541_002200"
     CDS             complement(16373..17278)
                     /locus_tag="PC541_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2839663.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT         6800 a         1966 c         3029 g         5722 t
ORIGIN      
        1 acaaaatcaa gatggaacta tagatataat aataaagaat gttacgtttt atgaagaaaa
       61 gtaatttata tacttagtat ataaattaaa tataagagcg tgtattttat atattgtaaa
      121 taaatgtaat ctagtctgtg cgaactctaa tataatttat tatgtagaag tttacacaga
      181 ctatttatat ttgtagacaa aacgatacta tttgttttct tcgatatatt tacatatatc
      241 accaatacat ttaaagttct cagcttcctc gtctggtatt tctattccaa attcatcttc
      301 aagggccatt ataatttcaa cagcatctaa tgaatcagct tctaagtcgt ccattaaaga
      361 tgcttccata gttatctcat ctaaattgtc tacccctaat tgttctgcta ttatttcttt
      421 tattttctca aacattttaa gcctcctaaa gcagttttaa ttaattttaa tgttagttta
      481 atataatatc aagaattttt caatatggaa tctataaaaa ataataaaat tttataaatg
      541 tagtaataaa gtattatttg atgaataaac tttattgata acaaatataa cttaggagga
      601 aatgctatga aatatgtagg actattacta acttcagtag gaatgttttt acttatagca
      661 gtaaattttt attataattc aataacttta gatatgcaac gtatagagga ttatgtaatg
      721 gagaccaatt taatccttga agatgtagct gaaaaggaga gttatgtatc taatgaaaaa
      781 gaagactaca tatcaaggtt aatgcatgta aagaaaggaa tagaaaattc aaaaacatcc
      841 ttcttgatag aaagatataa ggagtacaaa ataaaatcaa tagaaagttt aatctatact
      901 atatcagagg agaaaaaaga ttatctggat gaagttgata gatacaataa acttggtgaa
      961 aaagaaataa ataaactaat caataaaaac tttttagaag taacttatct gtctattact
     1021 acatatattt aattaaagag acaaaactta gtctcttaat gattcaagga ggattagtta
     1081 tgacaaaatt agaagacaat atggaacgtt tctttggagg atgcgaagga ttttttggga
     1141 ataaaatgtt gataataata gttatagtat tcttactatt atgcacagac attttagaag
     1201 acttattatg cgatgacaat atatggatat gggttatatt aatagtattg ttattattta
     1261 acttcgatga tggttgttgt tgctaaaaaa tattgattat taaataggta aaaccagtaa
     1321 cttatacaag ttatggtttt acctttttta gttcaaatta aaaaatttaa aaaaatagta
     1381 aaaatacatt agcaaaatgg aaaccaaatg catagtatat gttaggagcg aaaagctcaa
     1441 caaaatatca acaggaggta acgtataatg ttagatagat tctttggaga agaaggatgt
     1501 tttggtggag gaggatggtg gataatagta ctattcttcc tattcttagc ttttggagat
     1561 agctgggcag aatttgacat aatggcatgg ataccattcc taatagtttt attaatactt
     1621 tgctgctgtg gtggatgctt agaataagaa cacagttatt aaaaaaagga gggctttgtt
     1681 atgttagata gattttttgg agaagaagga tgctttggtg gaggaggatg gtggataata
     1741 gtactattct tcctattctt agcttttgaa gattgctgga gagacattga cataatgtca
     1801 tggatacctt tcttaatatt attgctaata acttgttcat gtggtggatt ctttgatggt
     1861 gaagtaggtt gcggctgcta atacttaaat cattagaata aaaaaatgct ggtattttat
     1921 accagcattt ttttatttta ttttttaata tgatttaaat taagaatgaa tattattaaa
     1981 tttgttgaat atagggtata atataaaata taatgtattt aaatgtactt gcttttatgg
     2041 taaaataaaa acttatataa atgtagttaa aataggaacc ttcaaaaatt tggttcagtt
     2101 ttagataaat ttagtaaaat aaggaggatg aagatgaaag gcaatataac gataaaagat
     2161 gttgctaaac aagcaggagt gtcaatatct actgtatcta gagttataaa tgattcaaaa
     2221 cctgtaactg atgaagtcaa acaaaaagtt ttagaggtta taaaagagac tggatatata
     2281 ccaaatccac ttgctagaag cttagtaaca aagaagagtc aattaatagg ggtaatagtt
     2341 ccagaagttt cagattcttt tgttaatgag gtgttaaatg ggatagaaga ggttgctaaa
     2401 atgtatgact atgatattct tttagcgaat acatactctg ataaggaaca agaacttaag
     2461 agtataaatc tattgagagc aaaacaagtg gaaggtatag ttatgatttc atggatagtt
     2521 gaacaagaac atatcaacta tatacaaaat tgtggaatac cagcgacata tataagtaaa
     2581 actgctagaa attatgatat atatacagta agtactagca acgaagaagc tacttttgat
     2641 atgacagagc atcttataaa gaaaggtcat gaaaagatag cttttataat gacgagtaaa
     2701 gatgatactg ttttagaaat ggaaagactt gctggttatg agaaagcact ttcaaataac
     2761 aatatagaat tagacaagag tttgattaag tatggtggaa ctgattatga gagtggatac
     2821 aatagtatga aagaactatt agatgatgga ataatacctc atgcggcttt tgtaacaggt
     2881 gatgaggctg ccataggtgc tataaatgct atatgtgatg ctggatataa ggttccagaa
     2941 gacatatctg ttgcaggatt taatgatgtt aagatagcta gaatgtatag acctaaactt
     3001 actacagtat atcaacctct atacgatatg ggagcagtag caataagaat ggttataaag
     3061 ttaataaata aggaattaat tgagaataag aaaatagaat taccttatag aattgttgat
     3121 agagaaagtg ttacagaaag aaaaaaataa aactattaaa atcaatcagg tagtgcctaa
     3181 ggcactacct gattttttat gtcaataata tcaaatttaa taatagatta actaaaaata
     3241 attatctaat tacttaaata gtttattttt atctattcat gagggttaga ctttttagtt
     3301 ttcttaaata aacttttaga ttttttttgt tgactacctg gtgtattttt tggtgcaggt
     3361 gaatttgact tacctttagt aggtttagcc ttcttagttt tagctggttt agtatctctt
     3421 tggttttctg catctgagat tttttccatt atattatcca gttttttgcg ttgattttca
     3481 tctaaaaatg ctttgaattt tgatactact tcatctctgc catctaggtt cttacctatt
     3541 tttataagtt cctccataag ttcattttct gattttcctt tgtaactatc agccatcttt
     3601 tctattttat ccttgtcaat atttttttgt ttagccattt tttctaatga ctctgtatct
     3661 aattttttca taaaaaaacc cctttctata aatatatatt gaattatatt catactattt
     3721 atatatagaa ataatattga agtggtgaat tgtgagtgat atataatata aataaaaatt
     3781 cttatagata atatatatgc actgagcttt aaaaatggaa cattaaaata tgaaaaacat
     3841 actttaatat agggcaatta tataatataa gggggaattg tcttgaataa cttactattt
     3901 ttgattggag tgattgcatt gagtaatggt aatatctcta tatttgatga taaaaataaa
     3961 tcaaaaggaa ataatagagt tagaaaaaac aaaaaaaatg caaatgataa actttcacag
     4021 tctacacatg gcaatttaag gtttgatgca aatgatataa aaagaggttt aaggctcatg
     4081 gatttaagtg aagaagattt agaaatggga atggaaataa taacaagaac taaaaaatac
     4141 atgtcaagag atgaaagaaa aatactaata aaaatagaaa gtgttttaga tttagttaga
     4201 ggaataaaaa aattaaataa tatagatatg gtagatacag aagatgaaac ggattttttc
     4261 agaaaaatgg atagtgatga taaaaagaat atgatgataa aggaaattat agatgtattc
     4321 ccagaaaaaa gaaaaaattc agttgaaaaa gctataggaa tgaaaaagaa gatagattta
     4381 tttgcagaat tatttttacc agatgacttt ggtgaaggtg gatttagttt gagctctttg
     4441 gcaaatataa acaatcttgg tagtatgaat aatctcaaat tgttaggaag tcttttaaga
     4501 ggtgatgata gtaataatga tgatgaagac aataatgatg agttaaatta tgaagacgaa
     4561 gaattagatt gtgaagacaa agaattggat gatgattatg atgagttaac agacgaaggt
     4621 gaaatggagg ataatgaaga tgaattagta gaagataaga acacaggcta tgaaaatata
     4681 gaaaatgatg aagatatagt atatgatgag gaactataca acattaatga gcaagaatcc
     4741 gaatacaatt ataatgaaaa aaatactaaa actagagcta agaaaaaacg ataattaact
     4801 aaatacataa agaaaaaagg aagaaaaagt aatgatagaa taagattatc tcaattacta
     4861 tataaaacca ctttaaaaaa ttcaaaattt attatataat aataatatga aatcctgaaa
     4921 catacgataa ggctttttta aattcaacct acaaatgaaa tacgggagac cgtgtctggt
     4981 aaattataac atgcctagtt agtttggacg ttaaaggtta ctggcaagtc acccacctgg
     5041 gagtgcctag ggtgtgaatt tacttagcca ccggtgtttc gggacaaaaa aagtgctact
     5101 atttcaaata gtagtacttt ttttattaat atgcattttt ctactaaata cgcctaatta
     5161 ttgataaatt caaaaagcaa gtaacaatta agattataaa agcatagtat taattaggct
     5221 tcaaaaagcc aaagacttaa taagaatcgc ttacagggag ggcaatgaaa aatgcaagat
     5281 tataaaaaaa ataaaagaag aatgatgaat cagccaatgt ctacaatgaa tgaagaagaa
     5341 gtgtatacag atgaaataaa ttcagaagac atgagaggtt ttaaaaaatc acaccatcat
     5401 aatggatgta atactgataa taagtgtgag tgccatgatg attgcaatcc atgcaaccca
     5461 tgtaatccat gtaaacctaa cccatgcaat ccatgcaaac ctaatccatg tgatgacaat
     5521 tgtggatgcc atgacaattg taaatgtgat tgtgaaccat gtgaaatgga ttcagatgaa
     5581 tgttttgaaa acaaatgtgg accagaatgc tgtaatccta tatctccaag aaacttctct
     5641 gtatcaaatg cagtgccatt tgcaatagag gctaatagaa tatttgatac tatgcaattc
     5701 caaacattta cagatgcaac aggaccaaat ggagagccat taacttttga aacagaagta
     5761 gtagaagtat ttggttcagt tccaagtgca ggtcaagcaa gtgtaactat agaaaaaata
     5821 tgcttaagta atgatggaat cgttatagac acaggaatga caactttaga agatttcgat
     5881 ttagacccat taggagatat agtaggaaga aactgtgaaa caacttttga atttgcagtt
     5941 tgtggagaaa gaaacgctga gtgctgtaga caaggaaaag gcaaatcagt agcttataaa
     6001 caaagaggat taactgtagc agttcgtaat ttagtactag agctaagagg tagatgtgga
     6061 tgtacagagt tcgttgcatt agctttccca gcagttagag caggaggtgg atgtaagaga
     6121 agagttgatt atgtagaatt tacttttaac acactttcag caccaatatg cttgccagct
     6181 gacggaagag ctgttacttt aagacaagaa tatcaaacta acttaactgt agattgtata
     6241 ggaaaatcta tattaaaatt agaatgcaac gaatgttgtg aacctttcta tgaattaatt
     6301 ataccaaatg atatagattt agtactttgc ttacaagaaa cagttagcac attaataagt
     6361 gaacaaatag tagttttagc atcaccaaat ccaatccaac caagacttgt tgatactttc
     6421 tctaaagtat gtgatttttc gcaatgtgga cctaatcatg gaagtggaaa gccaagttgc
     6481 cacagataga taggtaagat atatagacag tttaaattat aaataaaaat catctaagga
     6541 aggctaccaa agtgtataat ttacattggt agccttcacg tttttataac tagcgtatag
     6601 attttagtga tttaaaaagc actatagtat gtaatagaca agtaaagatt gatactatcg
     6661 tcattgtgta tatgtatgca taaatattaa gtcctggaat tggaagaaat acatataaag
     6721 ctattagttg aattaacatg ccagtaatag tatttatact tgaagctaat tcttttctta
     6781 ttgaatgtaa aatgccagat agagtttgat ttagggacat aaatagtgga actaaaaaca
     6841 tagcttttag atattctcct gcaagtgtat ttttaaatac taatatacat aaatcttttc
     6901 caaagaaata aaaaaatatt gatgatagta tgcctacaaa taaagtaaga agtatggagt
     6961 atttgatttt tttaattaca ctcttacgct ttcttagagc catttcttgt gaaatagttg
     7021 gtattaaatt tacaataagc gctgaaccaa cagtaaaagg taaaaatata aaaggcatta
     7081 ccatcccact gacaatacca tacatactta aagattgttg ataagtcatt cctgataatg
     7141 ctaatcttga tggtatcatc atagaactta tagattgcaa aagtatgttt gacattctat
     7201 tacaagttat gggaatagac atttttaatg tttccataga agaattataa aaatctttta
     7261 tgcttataat atacctattg tcaaatgatg aatctcgcca taagcaaata agcataaata
     7321 taatattact caactcaccg attgatatac ctaatagagc tatataacaa ttcatagatt
     7381 tattatttat gtacataact agtaaaaaaa caaataatat tttaccaatt tgttcaatga
     7441 tttgtccaat agcagggatt tttacattct ttatgccata gtaatatcct cttagtacgt
     7501 ttgaaagagt tattataact atagcaggac aaacagctag tataaataga tttaatttag
     7561 catcttttaa aaatttaagt gataaaaaag aactattgaa agatgcaact atagatataa
     7621 ttagtgctac gaaaaatgat atatataaag ttgatataaa taaagcatta gtattgtgtc
     7681 tatcgtttaa agcttttctc tttgcaacta gacaactaag tgcagtaggt atacctgttg
     7741 ttgtaacagc taagcaaatc atcaaaaagt taaaaatcat atgatagata cctaaacctg
     7801 tttcacctag tgctcttgat aagaatattt tatataaaaa accaaatatt cttacaataa
     7861 aattggatat aaataaaata actgttgata gtactaaata aggtactttc aaaaaaatca
     7921 cctccttatc ttttaaatat attcacaagg aggtgtaaaa atttataatt ttataaattc
     7981 tggtttcaat ttattaaatt tagatacata tttaaatccc atatttttta gtaatgaata
     8041 tatgtagtca aactcacatg caacttgact tatatgatgt gaatctgaac ctgtggttat
     8101 gatttcccct cccaagtctt tatacatttg aagtatatac ttagatgggg ttaggtctgg
     8161 aagattatat ctataacaag aggtatttat ctcgatacct ttgccatcat aaatagcttg
     8221 tttaagagtt tcttcaatta tatcagaaaa tagtctatca tctaaaatag tgtcataagg
     8281 tgcatacctt ttaatcaaat ctaaatgacc taagacagag taatctttat aattcttaac
     8341 aatattatat aaataaagat agtaattttc ataaacttca tgttgagttt tatctttaaa
     8401 ataattacca aggtataagt ccattgtatc tatagcatgg atagaacata ttataaagtc
     8461 caatggatat tgatgtgctt cctttttgta tgtatccatt aattgagttt gaactccaaa
     8521 ttcaattcct ttttttattg atattttatc tttatatttg ctttgaagag tctctaaact
     8581 cttaaaataa tcttcgtaga caattgaaaa agaatcatca gcatatacat catagtcaac
     8641 atggtctgtg aaacaaattt cctccaaacc gagttcaata gattttttta ccatttcttc
     8701 cattgtagat tttccgtctg tagagaaact tgagtgcatg tgatagtcgt aaaacataaa
     8761 tatttcccct ttttataaaa attttggtat tatatataca tactaaataa tatcaaatat
     8821 acataaaagc atcaataaaa tataaataca atgagcggtg ataaatttgg aaaaatacag
     8881 aatagattta aatgagaaaa aatcaactaa atatatacag atatttaatc atataaaaga
     8941 attgataata aataaagagt tagtagaaca tgaaaaattg cctccaataa gaaaactatc
     9001 aaatttttta caagtaaata acactactat agttaaggta tacgagcttt tagaaaatga
     9061 aggatatgta tataaaatta tagggagtgg tacttttgta tcaagtttaa attcttataa
     9121 aagcggtata gataacagga aaaataagat tattgtgcaa agagaagatt taattcattt
     9181 tgacaatggg aatccatcta atgatatgtt tcctatcgat tcatttaaaa atgctgtaaa
     9241 tatggcatta tctaaagatg gtagttcaat atttgaatat gatgaaggtc ttggctctga
     9301 agaattaaga gaaaaaatgg tagagtatct aagtgatgga aatataaaaa ctactaagga
     9361 aaatattcaa ataatatcag gtgctcaaca aggtatagat atagtttgta aaggacttat
     9421 aagttattca gatgttgttt ttatggaaga accttcttac aatggagcaa ttgaagtgtt
     9481 taaaagtaga ggtgcaaaaa taatatctat acctatgtta gatgatggaa ttgatatagg
     9541 tattttaaaa ttaaagttgg aaaaaataaa accaagactt atatacatta tgcctaattt
     9601 tcaaaatcca actggaatat cttactcaac atataaaaaa aagaaattga tagaattggc
     9661 agaagaacat gatttttata taatagaaga tgattttata agtgattttg tttttgaatc
     9721 aaaggacaat agaacaatta gaagttatga tgataaaaat agagtagtat atataaaaag
     9781 cttttcaaaa atacttatgc caggtcttag gataggaatt gtagaaatgc caaatgagtt
     9841 gttaaaaaaa gttttatggg ctaaatattc atcagatata tcaacaccag gtttaattca
     9901 aaaatcaatg tattactata tgaataattt tgattggaag aattatttga gtcatataga
     9961 aaagatatat actgataagt ataaattggc aaaaaacttg attaatgaca aattgagtgg
    10021 taaattaaaa gttagaaaat cttgtggagg aataaacttt tttttagaat taccaagagg
    10081 atatacatct caagcattta caaattttat gttaaacaaa ggagtgtcaa tgcttccagg
    10141 gacttatttt tttgacaatc tagtggatga tagattcttt aggataaata tagctcgccc
    10201 aagcatggaa gaactagaaa agggaatatc tataataagt gataatatag atgagttttt
    10261 ttgtgaatat aaaaacaagt tagatataaa aagcaataaa ttattttatt aaataaatag
    10321 aggtttttta ggaggaaaaa atgtttgaca agaagaaatt agataggatt aatgaattag
    10381 caaagaaaaa taaagaggga atcctttctg ctgatgaaat caaagaaaga gaaattttaa
    10441 ggaaagaata tttagaaaac tttagagccc actttagatc aagactagat agtgtcaaag
    10501 tagtttcacc agaagaatat gagcaataca tgaaaaacaa taaaaattag aacttagtag
    10561 gaggaaaata tgaacttaaa gtacgaattt aagaatgctt gggattttga aagaaaagaa
    10621 aatactattg aacaaattat gcaatattct agcaattata tggagttttt aagtaagtca
    10681 aagactgaaa gactttcagt aaaagaaatt ataaaattgg ctaaagaaaa taactatatt
    10741 tcaatagatg aagctatgga aaaaggaagc ataagttgtg gtgataagat ttatgttata
    10801 aataaagaga aggcagtagc actttttgta ataggtaaaa attatataga aaaaggtatg
    10861 aagattatag gaagccatat agattcgcca agattagatt taaaaccaaa tccactgtat
    10921 caagagagta atctaggttt ttttaagact cattattatg gtggaataaa aaaataccaa
    10981 tggacggcta ttccacttgc tttacatggt atagttatat taaatgatgg aacaaaagtg
    11041 gacatatcta ttggggaaga agatagtgac ccagtatttt gtgtaactga tttattgatt
    11101 catttggctg gagaccaaat gcaaaagaaa ctatcagaag gaattagtgg agaagcttta
    11161 aatatattaa ttggaaatat gcctttagaa gatgaagaaa aagaacctat aacagcaaat
    11221 atattaaaga ttttaaatga gaaatacaat atagttgaag aagatttatt aagtgctgaa
    11281 attgaagtag ttccagctgg taaggctaga gatttagggc ttgataggtc tatggtgttg
    11341 ggatatgggc atgatgatag ggtatgttca tatgctgctg taaaagctat actagaaact
    11401 gaacaaccag aatttacttc agttgcatta tgtgtagata aagaagaaat tggttctaaa
    11461 gggaatacag gtatgcactc aaagtttttt gaaaatactg tggctgaact gattgcttta
    11521 gaaggagatt attgtgatat taaagtaaga agagctttag caaatagtaa ggtattatca
    11581 gctgatgttt ctgctggata tgacccaaat tttggagaag catatgaaaa gagaaattcg
    11641 gcatatatgg gaaatggagt agttttaact aagtatacag gttcaagagg aaagagtgga
    11701 tgtaatgatg ctaatgctga atttatgtca gaagtaaaga gaatttttaa taaaggaaat
    11761 gtaatatggc aaactgctga acttggaaaa gtagaccaag gaggaggcgg aactatagcc
    11821 catatattgg caaatcaagg tgcagaggtt atagactgtg gtgttggagt tttaaatatg
    11881 catgcaccac atgagattgt ctctaaagta gacatatatg aaatgtataa gggatataag
    11941 gcatttttta atataaattt ataagtaaaa atataaatgt aataagtgaa taaaggctgt
    12001 tgtataaaca atttaatctg ttgtacaaca gtcttatttt tatgtaaaaa tagcaatttg
    12061 tcacactaga gaaaataaaa gaaaatactg tcaaattaaa aattatttaa aaaaaaattg
    12121 acaacgattt catcttgtta taatatgtaa taaagaactt gttatataat gttataaaaa
    12181 tgttacatag ggaggtgagc aaaatggatg aaaagagaca aaaaaaagaa atggatttag
    12241 aagtaaattc ctttgttcct ttatatcaac aactatatga caacataaaa aaacaaatag
    12301 catctggcat atataaacca ggagataaac ttccatctga aggagactta tgtaaagaat
    12361 ttaatataag tcgtataact gtgagaaatg cacttaatga acttgtaaaa gaagatatat
    12421 tatgtaaaaa acgtggtaaa gggacttatg taacaatacc agagagaata gaagcaacat
    12481 gtgcaggaaa tagttttact aattcttgtc atcgtattaa tgcaaaacca agtaccaaaa
    12541 taatctctgt tttaataaaa aaagcagata agcaagtagc tgaagcttta agtattgaac
    12601 tagaagagaa ggtaatatgt attaaaagac ttagattaat agatgaagta cctgttattt
    12661 ttgaagttga ctattttaga atagattaca tgtttttatt aaaagaagaa ttagaagata
    12721 aatctttaat ggaggttata tctaataata taagcacatt acctaaacga gtagaaaata
    12781 tatttgaagt aaaacattca aacaaagaat attctgacca tcttaaatgt gcaagtaata
    12841 tgccactatt gaaagtaaga caatcggtgt atacagaaaa taatgatgtc ttatattaca
    12901 atgagcaatt tataagaagt gataagtaca aatatgcagt atcagcagaa atataatcta
    12961 taatttagta ggaggatgag tatgaaaaag ataatattag catctcatgg agacttttca
    13021 aaagggcttt taaatgcagt aaaaatgata gtaggagatt tagcagattg tgtcagttcg
    13081 tatggtttgt accctggaca aagtgcttca gattttgctt tagaattaga aaaaacaata
    13141 tcagaagata aagaatgtga atatataatt ttatctgact tatatggagc aagtgtatgt
    13201 acagcaatgt taagattaac taatctacat aatgttaggt tattttctgg aatgaatttg
    13261 aatatggtat tagaacttct tacaaggttt tctgatgaat taacagttga agatatagac
    13321 caattagtgg aagaatctag aagaggaata caaagcgtga cattacaaat taaagaagaa
    13381 gaagaggtat tttagtttat gaaaaaaaag ggaatattat cagtatttaa aagtaaaaaa
    13441 ccaattatag caatgataca cttaaaagga gatactccag aagatatctt tgaaagagct
    13501 aaaaaagaga ttacaatctt tgaagaaaat ggtgtagatg gaataatgtt agaaaattac
    13561 tatggtaatt attatgattt agaaagaata cttgaatatg tatcaaaagc taatttatca
    13621 ataccatatg gagtaaactg tttaaatgtt gatactatgg gatttgaact ggcaacaaaa
    13681 tataatgcta gttatataca ggttgattct gtagtaggtc atgttaaacc tagagatgaa
    13741 gctactttag aagagttttt taaattacaa cgttcaaaat gtccagcgta tttaattgga
    13801 ggcgtacgtt ttaaatatca gccagtatta tcagaaaatg atgttgaaga agatttaaaa
    13861 ataggaatga caagatgtga tgctatagca gttacagaaa atgctacagg tcaagaaaca
    13921 tcaatggaaa aaatagaact gtttcgtaaa aatttaggag acttcccatt ggttatagct
    13981 gctggagtta cattagaaaa tgcaaaaaaa cagcttaaat taggagatat ggcaataata
    14041 ggaagttact ttaaagataa ttacaaagat tttggggatg ttagtgtaga acacgtaaaa
    14101 acattcatgg atgaaatcaa aaaaattagg gaggaattat aatgattaaa ttagtaagag
    14161 tagaccacag acttatacat ggacaagtag catttacatg gacaaagttt ttaagtacag
    14221 actgtatatt gatagcaagt gatgacttat taaaagatga attaagaatg gcaggactta
    14281 aaatggctaa accatctaat gttaagttag taatgaaaag catagcagat tctataaaag
    14341 cacttaactc aggtgttact gataaataca acttattgat actttgtgaa tctgtagaag
    14401 atgtttacag acttgctaaa gaggtaaaag ctattaaatc tataaacctt ggtggaacaa
    14461 aatcagatga taatcgtgaa aatatatcta aagcagtgca tgtatcaaaa gatgatataa
    14521 agatgattaa agagttagat tcagaaggcg taaatgtatt tgtacaatta gttcctgatg
    14581 atgatgctac aaatgtaatg aaattaatat aattttaggg ggatgaaaat ggaatttaca
    14641 caagttattt tgataactct aattgcattc tttgcatata tgcatagttt tgtaggttct
    14701 acaatgcata atagacctat agttgttgca ccacttgttg gtttagcact aggtaatctt
    14761 catacaggta tagtaatagg ttcaacatta gaattagtat ttatgggtgc atttcctgtt
    14821 ggggcaagta atcctcctga ttttgtgtct ggttctatta ttgccacagc gtttgtaata
    14881 ttaacgggtc aagatgtatc agcagcagtt gtacttgcag ttcctatagc aactctagta
    14941 ttgcttatag ataacttctt aatgacagta gtacttacat ggggagctca tattgctgac
    15001 cgttatgcag aagaggggaa tattgaaggc gtagaacgtg tgcaattatt atttggtata
    15061 ggaaataaat taatattagc aataatagta ggtataggat tttcattagg tgttccagta
    15121 atagaaaaaa tactttcatt tataccatca tatgtaactc atggaatgga tgttgcagca
    15181 ggcgtgattc cagcaatagg ttttgccatg ttagctagaa tgatgcttaa taagaaaaca
    15241 gtagcatttt tattattagg atttatattg gttgcttacc taaacataac agtaacagga
    15301 gttgcattgt ttggattagc aatagcttta atatatgtta actttgtagg agagaaggag
    15361 gtaatagtag atgacaacga gttctaagaa attagagaca atttcaccag atagtaaaat
    15421 aacgaggaaa gacttttgga aatgttttag aaggtcattg acattagatt catcttggaa
    15481 ctacgaacgt atgcaaaata tagcatatgc atacatgatg gcaccaataa tccgtagatt
    15541 atataaagat gataaagaaa agaaatctaa ggctttaaaa agacatttag aatttatgtc
    15601 agttactcct catataagta ctcttttggt aggtatatct ggagctatgg aagaagaaaa
    15661 tgcaaagaat aaagaatttg atgctaatag tataaatgct gttaaatcaa gtttaatggg
    15721 tccagtttct gggataggag attcattttt ctggggaaca ttaaagctta tagcagctgg
    15781 ggttggtata gctttagctt ctcagggtaa cataatggga ccgattttat tccttttaat
    15841 aataaatgtt cctcatttta ttatacgcta tatatgtcta gataaaggat ttaagtatgg
    15901 aactcaattc tttaaagatg tgagtggttc aagtatagtt tcaaaagtta tggaagcagc
    15961 ttcaatgtta gggcttatgg taattggagg tatgactgca tctaatgtaa tgttaaaact
    16021 tagtgttaat gtaggtagtg gagaatgggc agagcctatt caaacttact tagaccaaat
    16081 aatgccatgt atgcttccag ctatgatatt cggtattatg tattggttat taggaaaaaa
    16141 ggtaaagaca actacgatat taatttcagt tatgattatt tgtatagtat tggctgctat
    16201 tggagtagta taaataaaaa aatcccaagt tcatttgaac gagggatttt ttgactaaat
    16261 taaataaaaa agaaagatgg ttcataataa tttataagca tttatgtgat agttataatt
    16321 agcttattaa ttagttataa actcaaaatt aagcactact ttattaaagt tattatatta
    16381 taatttcgaa atactcacat aatctgttta gaaatttatc ttctaatggg gaaaaagttt
    16441 tctttttaga gtaaatgaag aaaaactttc tagtaaaatc aacatccttt attttataac
    16501 attttatttt acctgcattg agataatcga tagctgaaat gtacgatata aaggacactc
    16561 caagacctaa tctaaccatt tccttaatag cctcatttga ttctacatga gcgattacat
    16621 ttaatttact tactggaaag ttatttttac taagtgtgtt tagtattaag tttcttgtac
    16681 ctgacccttc ttttctcata ataaaattta gataagccaa ttctcctata tcgatacaat
    16741 tatttttatt atcaatttta aaatctgaag gagtgataag aacaagttca tcatctaata
    16801 agtctaggta ctctatttga ggattattga ttttagaacc aactaatcca aagcttattc
    16861 gttcatttaa aatttctgat atagcatatt gagaatcata atggcttatg gaaaatttta
    16921 catctggata actcatagaa aaactcttca taaaatcagg taaaatataa gtttcaggta
    16981 tggaactaca tgctatatct ataatacctt ctatttttcc tgaatattct tttatatcat
    17041 aaattgcacg tttgcaatta ttcaatatat atatagcatg ttcataaaga atttcaccag
    17101 attttgtgag tgtaataact ttgttgcttc tatttataag aacagtttct agctctctct
    17161 ctaaattctg tatgtgtgcg cttacagtag gttgagttaa aaaaagttct cttgcagcct
    17221 ttgaaaaact ctgatgtttt gcaacagcaa caaatacttc aagttgttta aaatccatta
    17281 tttaatcctc cttaaattat ctaaaactat tataacatat ttgataaatt aatagtcaaa
    17341 ggtgattttt aaaatagaaa acctttaaat attcatggag tttttgattg tttaggaata
    17401 atttggtata atgttgtaag gtacttacaa taaaaatatc gtaaattgaa ttttcaaaat
    17461 tcaactttaa agtgtttttt attacagaaa aattacaatt agcttcttaa tcttatt
//
LOCUS       DAMJZE010000080        17395 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJZE010000080 DAMJZE010000000
VERSION     DAMJZE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.080"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            121..867
                     /locus_tag="PC541_002201"
     CDS             121..867
                     /locus_tag="PC541_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2839664.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            928..1602
                     /locus_tag="PC541_002202"
     CDS             928..1602
                     /locus_tag="PC541_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2839665.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            1970..3316
                     /locus_tag="PC541_002203"
     CDS             1970..3316
                     /locus_tag="PC541_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2839666.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3343..4743
                     /locus_tag="PC541_002204"
     CDS             3343..4743
                     /locus_tag="PC541_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2839667.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4953..6389
                     /locus_tag="PC541_002205"
     CDS             4953..6389
                     /locus_tag="PC541_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2839668.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6733..7677
                     /locus_tag="PC541_002206"
     CDS             6733..7677
                     /locus_tag="PC541_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2839669.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7846..8778
                     /locus_tag="PC541_002207"
     CDS             7846..8778
                     /locus_tag="PC541_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2839670.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8883..9350
                     /locus_tag="PC541_002208"
     CDS             8883..9350
                     /locus_tag="PC541_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2839671.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9594..10448)
                     /locus_tag="PC541_002209"
     CDS             complement(9594..10448)
                     /locus_tag="PC541_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2839672.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10614..12578
                     /locus_tag="PC541_002210"
     CDS             10614..12578
                     /locus_tag="PC541_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2839673.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12578..13039
                     /locus_tag="PC541_002211"
     CDS             12578..13039
                     /locus_tag="PC541_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2839674.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13042..13362
                     /locus_tag="PC541_002212"
     CDS             13042..13362
                     /locus_tag="PC541_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2839675.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13378..14478
                     /locus_tag="PC541_002213"
     CDS             13378..14478
                     /locus_tag="PC541_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2839676.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14598..17198
                     /locus_tag="PC541_002214"
     CDS             14598..17198
                     /locus_tag="PC541_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2839677.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6922 a         1787 c         3008 g         5678 t
ORIGIN      
        1 taatttcaaa ttaataaata attagatttg ataaacaaaa aataaaaatg ttgattatat
       61 taataaactt taatataata tatttatgaa aaatgtaaac tgtgaaatgg aggtagtaac
      121 atgaaaattg aaaatctttc tattgatgat attaaacgag gctatatatt taatgatgat
      181 ttaaattgtt atagttgcat taaatgtaca aaaacatatg aggtaggaga aatatataaa
      241 ataggtaata gattctttga agcttcaaaa gctattgaac ttcatatgga attagaacat
      301 agtgattatt tagagcaatt gcttaatagt gattcaaaat acaacacatt gactgaaaat
      361 caaaaacaat tatttcaatt atttattagt gatttaccag ataaggagat agcaaaacag
      421 gttggagtat ccacatctac tatacgccat caaaaattta ttttcagaga aaaagcaaaa
      481 caggctaagc tctatttagc actatatgaa tctgtatttg aagataaatc taataaaagt
      541 agtactataa taccaatcca tgaacatgct cgaatggtag atgaaagata tgtagtaact
      601 gaagaagaga gaaaacacat tttggataca tcatttgaaa gtatgaatcc tttaaaatta
      661 aaagtatttt cttcaaaaga aaagaaaaag gtcgtaattt tatctaagat tttggaacaa
      721 tttgaacgtg ggaagaaata ttcagaaaag gaagttaatc agattttaga atctatatat
      781 gatgattttg caacaattag aagatatcta attgagtatg gttttatgag tagaaataaa
      841 gaatgtacaa aatattggtt aacataatta aaataataaa ttttactaat gtgtaatgtt
      901 atttttatct agggggagga gtcaaatatg aatgatgaat taaaatctat tttatacaat
      961 aaaggtgtgg acattgttcg ctttgttgat atatctgaat ttccaataaa tcaaacgcaa
     1021 ggtttttcta aagcaatttt attttgtata gggttatcaa aaaaatttat tacagatata
     1081 tacaataact tacctacaga tagtgatgag tttttagaaa aagaggagaa agcagaagaa
     1141 ttagctgact ggatttcaga atatattcaa aataaaggat atagagcgta ttctcaatcg
     1201 gagaaaaaca atctagaaca tggatatttt gaaaaagcct atattaatcc agaaatgcaa
     1261 tcagggatta gtcctttacc acataagaca atagcaaata ttagtggcat tggatttatg
     1321 ggaaaaaata atttgtttgt aacagaggaa tacggatgtg cttttagcat gtgtacagta
     1381 cttacagatg ctccaatttc tgttgaaaga tatcctttga ttgattcaaa atgtatggat
     1441 tgtaatgttt gtgtagaaaa ctgccctgca aaagctatac atggaaatga gtggactttg
     1501 ccaggaaaaa gagagtctat tattgatgtt tcaaagtgtt tttgtgtatt gaagtgtatg
     1561 atgagttgcc catggtcatt aagatatgca aatcaaaaat aattttacta gagaaggtat
     1621 taaattgaat attataaaat ttttaaaatg taagattata taagattttt caaaataaaa
     1681 atttgtatag aacactcaga aatgagtgtt tttttattgg aattaaatta tattgataca
     1741 aagtttaact attttaatca ttatataaaa tttgtattaa aaaattaaga ataacttggt
     1801 aatagtgaaa atataatgag attataattg atttataata taactataat gatgttgtaa
     1861 aatgaaaatt gtcatttcaa aattcccaaa aagagtaaaa gtcacttcgg ttatagtcaa
     1921 aaaatttttt cagtgaaaag aatttagatt taaaagggag gaattaatca tggaaattga
     1981 aactaaaact ccggattgga ttaatgtact agaattaaac agtaaaatag atattactgg
     2041 gaaatatgat gtaagtaata ttatacaaga tattgtctct gataagtcac tagaaggttc
     2101 cattatatat tttccaaaag gtaattattt atttgagaaa ggtattaaaa ttagtcaaca
     2161 aataacactt caaggagatt cttattatgg aggagataat caagtatcaa atttagataa
     2221 aaaacaatca atcataggaa caaccaattt tataactcga ggagtttcta atatgagtat
     2281 tattgcattg acaggaacaa gtcaatgtat caaaaatatt aatttttatt atgatagtca
     2341 tgatatagag aaaataccgc ctaaaaatgt atctgctatt acagaatatg gagaaacaca
     2401 aggactatct cattttgaac acctgtttat ttctggattt tcagggatag ggattgaaat
     2461 tccatactat tcaactggca atgatataat tgttagttca tgtggtcttg gtatgaggct
     2521 tggagagaag tcaatgcttt ctagtagcaa gatttacgaa tgtaaaaatg gaatggagat
     2581 tactactggt gttagcttaa ataatgtgag aatagaagaa ataaaagaaa taggtattaa
     2641 taataaagga tttggtttta acctgattat gaatcttatg gtcgaccaat gtggatattg
     2701 tggatttatg tttgaaaaaa tgtgctatag ccagataact gccagaatta ctagatgcgg
     2761 tcagtattat aaaagtgttg attataatgc ttatgagaat atggaagata gaaaagaaaa
     2821 agcttattcc attttttatg gagatatttt agaaagttgc aatattgtat tgatgaatag
     2881 aaatattgat accttagaaa gtgattcact taatgaacat aagatatatg taatcaaggc
     2941 aaatgaaaca aacaatgtaa tgctaacatg ttatgctgaa gcagataact ttatacaatg
     3001 tgcaaagggc aacttgctgt tagaaaatgg tgaaaatact tataaatttt ataatggtga
     3061 gatttgttct gtaggaggtg ttcaaatttc tgatatagat gaaaaagatt taatcaaaat
     3121 aatagagaac actttgtata tagatgataa aggtaagtat ttaatggttc ctagattaaa
     3181 aaagaatact gtaatatatt cagtactaga ttctacagaa gaaataaata aattacatgg
     3241 tggaatatgg gaacagattg atgtaaaagt attagctggg gaatccatgt actattataa
     3301 aaagataaaa gattaaatta tcaaactagg aggtagtgat taatggaaga gtctataata
     3361 attaaagata taagtggctg gattaatgta cttgattatg gaatttgtaa tgatgaaaca
     3421 aaagatgtaa gttttaagtt gcaagagatt atggatgctg taccagaaga ttctattata
     3481 tattttccaa aaggaaaata cttatttgaa agtggtgtta agattaagaa acgattaacc
     3541 ttatgtggag attcttatat tttagcgaat gttcctaaaa atactgctgg agtaacacag
     3601 tttactttta aaggaaagaa aaatcctaaa agtgaaaata gagcaatcat aacagcaaaa
     3661 agtgtaagac actgtattaa gaacatttgc ttttattcag atagttgtga tagagatatg
     3721 acatctaatc atgaacctcc aacagatgga aaacctagat ttcatcataa tgtaattata
     3781 aattatagaa atatatctgc tgttatatgt gaagataaac agggagaacc aggtcattat
     3841 gaaaatctat cttttagtgg tttttctgga attgcacttg atatgcctaa taattctaca
     3901 gcaaatgata ttacaatttt tacatccgga cttggaatta gtacagggga aaattcaaca
     3961 attgctaaca gtaaggtttg gggctgtgtt gatggaatga taatttctac tggaactttt
     4021 ataaatggta tgagggtaga agaaataaaa aaagttggaa ttaaaaatat tggtagaggt
     4081 ttaaatttta ttacaaatat cacaattgac caatgtggat attgtggagt ttggtttgat
     4141 tctatatcca atgttcagat atcaggaaat atcacaaggt gtgggcagta ctattatgat
     4201 gtaagctatg ataattattt aatgttggat aatagagtta aagaagcata ttctttattc
     4261 tatggtaatt tattggagtc aagtaatata gttttatcaa ataccaatga agataattgg
     4321 gaagatttca aagaagataa gcataaggtg tatcttatag aagcacttaa aaccaagaat
     4381 gtactattaa catgcaatat agatgctact gactggattg ttaagtcaga aaaaggaaat
     4441 ttaacgtaca ataatatgcg acaaactgtt atattttacg atggaaaact aagtagtatc
     4501 aatggaattg gtatatctga tgaagattat ggggataaag tcaagataaa aaaaggaaat
     4561 atatatataa atagtaatga taaggatatt aagatgcata aaccagaggt aggtgacatt
     4621 atgtcaacaa ttattaaaga taaaagtatt ctttctgctc attatggggg aacatgggag
     4681 aaaattggtg aaaaaataga gtttggaata actgtacact attataaatt gatagagaaa
     4741 taagtaaata cgtagtattt gaatagatgt attttattgg agagttagga ctatagtttt
     4801 aatataaatg gtttggtaat agcatggatt gaataatata aaaataatat aaaaaattaa
     4861 tttgattatg taaatttcta tgaagtagta aaactgtgac tttagtcata ggaggttcaa
     4921 accaagtaaa agggaaagga atatataata ttatggaagg aagaactggg attaaagatg
     4981 gaagatggat taatgtagta gaaagaggta ttaaaaatga tggttcgtac aacataggtc
     5041 ctgctttgca ggatattatt gatgaaatag aaacagattt ttgtgtctta tattttccaa
     5101 aaggtaaata cctatttaca agtggtgtta aaattaataa agaaattgta ttgcaaggtg
     5161 attctaattc atctacaggt actacacaat ttataactcg tggagtagaa aatatgagta
     5221 ttattacatt gacagggaag aaacaatgta ttaaaaatat aaatttctat tctgatagtt
     5281 gtgagataca ggtaaataaa gaaccaccga caaaaggtaa tcctaaatat cattatgaga
     5341 tgattattaa taagaatgaa aagaatgttg agatgaataa tgtatcagca atcctgtatg
     5401 ataacagtaa gaatatgatt aaaggtctag gtcattatga aaatatttat ataagtggtt
     5461 tttcgggtac aggaattagg ataccatatt actcaattgt aaatgatatt acagtttcat
     5521 cttgtgggct gggtattgat acaggaatag acacaattat ttctaatagt agaatatcta
     5581 agtgtcaaaa tggaatgagg attagaactg gaactagtat aaataatgta aggattgaaa
     5641 aaatacagaa agttggtctt ataagtagtc aagttaaggg gtatgagggg tatggaagtt
     5701 ataaaattaa taatattacg attgaccaat gtgggtattg tggcttttca tttgattcaa
     5761 tgaaagatag ttatttttct ggtattataa gaaggtgtgg tcagtattat tataatacag
     5821 attatgacac atatttaagt attaaagata gagtagaaga agcatattct ttattttatg
     5881 gaaattattt tacagaatgt aatattgatt tagttagtga taataaggat aactgggatg
     5941 atggcttaga atataaacat aaagtttatg tatttaaaac ccttcaaata ccaaaattaa
     6001 cattgagatg caatatagat gcctatgatt atatcgttaa atcggtacat ggaggaggta
     6061 atttaatgtt aaaaaataac tttaacaatt atatatttat tgattcatct aatccagaag
     6121 ttattaatgg aattaatttt aattatcaaa agaatggaaa cttgttaagg ttaaataatg
     6181 gtaaattaag ttataaaaat ggaaaggaag aaacagctat attcagacct aattatggtc
     6241 ttattgtagc atcaacagat tcagaatgtg aacaaataga ggctacctat ggagaaaaat
     6301 gggagaagct tggttctgaa gtaattggag aagaaacggt atattattat aaacatcatg
     6361 aaccagataa aacaaaccaa atacaataaa atttgtatta tataatgata taaagtttta
     6421 atatatagtg tttaccttta atgtaatatt ggtatatttg tatagtatta aatttgttaa
     6481 aaagtaaaat tgtagggtgc ttgaaaatag cacctttttt agtttcatta taaaattagt
     6541 tttgaaagtt taggaataat gtatatttat ttttaaagaa ttataacctt attaaggata
     6601 ttttaaatgc tagaaatgaa tcagtaaatc atactggaat tatcttaaat gttttgatta
     6661 aattaagtat ttagagctat aattatatta aaaatatatt agaacataat tatattacta
     6721 tggaggttaa atatgatagt aagtgtgagt agaagaactg atatacctgc tttctatagt
     6781 aagtggtttt ttaacagact tgaagaaggt tttgtttatg taattaatcc aatgaatcca
     6841 aaacaggtta gtaagataga gttaaatcca catactgttg attgttttgt attttggaca
     6901 aaggatgtaa ctccaatgtt atatgattta aataaattaa gagactataa atattatttt
     6961 cactatacta taacatcata tggaaaagaa gtagagacat gtattttaga taaaagaaag
     7021 gtaatagatt catttaagga attgtctaga aaaataggca aagaaagagt aattttaaga
     7081 tacgacccga ttttcttaag tgaaaagtac acagtagatt atcatatcaa agcatttgcg
     7141 agtttatgta atcagcttga tggatttaca gaaaaatgta taataagttt tatcgatttg
     7201 tataagaaaa caaaatttaa tactaaagca ttacatataa agccaataga aattacagaa
     7261 atagaaacat tatcaaaaga gatttctaag attgctcata agcatgggat aatacttgaa
     7321 atgtgttctg aagaatataa tttaagtaaa tttgggattg gaaaaagtaa gtgtattgat
     7381 gatagattaa tatcaaaaat tataggatgt gaagtagagg taaaaaaaga tagtacacaa
     7441 agagatattt gtggatgtgt taaaagtgtc gatataggac aatacaatac ttgcagacat
     7501 tattgtctgt attgttatgc taactttaat tatgcaaaag tagaagaaaa ctgtaggctt
     7561 tataaggaag ataataaatt actcatagat actttaagag atgatgcaaa aataagcatt
     7621 agagatatga agactattaa agtagataag aataaacaaa ttagtatatt tgattaaaat
     7681 aaatatacta attaccttaa tatattaaga atatagatac ttttgataac aaaattaaac
     7741 ttaatagata ttttctatat aagaaagatg gtcataatat gtcaatttga ccatcttttt
     7801 gatataatta aaacacaaat aatttagtaa caaaggaggc agaacttgaa tttattaaaa
     7861 aaatatccta tacctattgc aagtttgatt ttatcaattt ttactttagg taacttatta
     7921 caatcttata gtgaatcatt acgtaatgta attggattta taggatttat tttgtatgca
     7981 atttatatag tcaaaataat attacttaga aagggtgtta aagagcaatt agagaaccct
     8041 ttaatatcaa gtacatttcc aactataaca atggcaacaa tgatatttgc tacatatata
     8101 aaaccattat ctttagaatt aagtattatg atttggagca taggaattgt gggtcatctt
     8161 gtgttaatag tcttatttag taagaagttc ttagttaaat tctcaataaa aacggtattt
     8221 ccatcttggt atatagtata tgttgggata gttgcagcta gtgttacggc accagcattt
     8281 aatcaacttt tgattggcaa aattgccttt tggataggat ttgtatctta tatagttctt
     8341 ataccagtaa ttttaaagag agtatggata atcaaggaaa tgcctgaacc atccttacca
     8401 agtatagcta tattagctgc accagggtca ctattattgt ctggatatat taattctttt
     8461 tcagataaaa ataatgtagt attatacttg ttattcatat tatcaattgt attttatatt
     8521 atagttttgg gatatttacc gaaattatta aggttgcctt tttatccgag ttttgctgca
     8581 tttacattcc ctatggcaat aagtgcaatg ggtatgaaac taatgaatgg ttatttaact
     8641 aaatcaggtc attttataag ttggatttca tatgcttcta agttagaaga gataattgct
     8701 acagtgatta ttttatatgt tttagttttg tttattatat atcttagtaa atctgaaact
     8761 acatctaaag ctaaataaga attattaaat atgtgtattt aataggaata ttgatataaa
     8821 tatatgaaga taagataaac catagaaact taatttagct taattttatt ggaggaaaac
     8881 aaatgaaaac aaaagaagaa tatttaaaag aagttagaaa catatcagaa gaatacttta
     8941 gaaaaggaga gtttttttgc tctgaagcag ttctccagac aataaatgat gctttaggcc
     9001 aacctttaag tccagagata actaagttag catcaggatt tccgatagga cttggaaagg
     9061 cacaatgtct ttgtggagca atatctgggg gagagatggc tttaggtata gtatatggaa
     9121 gagttcatgg tgaagctatg aatccaaaga tgtttgagca tgctaaaggt ttgcatgatt
     9181 atattaaaaa agagtatggt gcaacatgtt gtagagctat tacaaagaaa tgggatggtg
     9241 ataactttat gtctcccgaa agaaaagagc actgtattaa aataacaggt caagttacag
     9301 agtgggttgc agataagctt atagaagatg aaaaacttaa tatcaaatag tgtaatatta
     9361 gttggtgaat ttataaagtt aaatttactt atagtataag atacttgata ttctcgtatt
     9421 gatagaaaaa tttatagcat atgtatttaa tgaaatatac ttatatttag agtgtggttt
     9481 aatttcataa atacatatgc tattatttta agtatataag caaatttatt taaataaata
     9541 gaaacagtta atttatatgt acaaaactat atatgattag tgtaaataaa tacctatttt
     9601 ataatactca acataaagac accagacatg attagtatta taccaataaa aatagcaagc
     9661 cattcttttt tgttcttctt ttctttaaaa aatataatcc ctcctaaagt ggctactatc
     9721 acacaactct gagagataga aaaacttatt gtaactccca atgtattagt cgttatgaac
     9781 atacctaaat ttgctattga ccacaatata ccagtaatta aattgaataa aacattttcc
     9841 ctatatacaa tctctttaga aaaatacttg acaataatag atgaagttat caacatacca
     9901 acagattgag gtaatataat tctatatcct tcaattaaaa atatttgatt agttacaaca
     9961 tataatgata aaaatagaga tgataataat acaactccaa cacattttaa catattttta
    10021 tctgtagatt ttttagtttg ataatttgtg agaagtatac caagtattat acataatatt
    10081 gataaagaac caatcagtaa cattttagaa ctggtccatt cattaaacaa gattacagct
    10141 aataatgtcg taaaagttaa ttgtgaacca tttgaaattg gcattacttt agatacacta
    10201 gcaatttgaa ttgctttaaa ctgcattaat tgtcccaaac tccagaatat gccagaaatc
    10261 atacttatac aaaatgaaaa aaatgtaatt tcaggatgta taaccagagt tattatagtg
    10321 gaaaatataa acgcacttat ggatgtacct aaaagctgtt catgaggaga acttttcttt
    10381 agatttgcaa ctatgggcat caatccccat cccattacag gaagtaaagc aataattatt
    10441 gtcagcatta aactatttcc tttcatatta aatttagaca gcttttaatt tatcatctaa
    10501 aattaaagtg ttcaatacat aaaaattacc gtatttaatc aggaaatatt agtagttttt
    10561 tagaaaaaca ttttccatta aaataaagac aaagggaagg agtaaaattg aaaatgttaa
    10621 ctgatatgat tcaaaatcgt gaaattaaaa taataaagtt tttattaaaa gaaggtcata
    10681 caactgtaaa agcaatagca gatgatttag atgtttcaga aaaaacagtt tcaaaatctc
    10741 ttaaagaagt agaagcagtg cttaaaaaga taggtctttc tttaataaga aaacctaaga
    10801 taggtgttta tattgatgga gatttaaaaa aaatacttcc ttacctagat aataaaggaa
    10861 gtcaaattcc tacaaccaga gaggagagag ttatctatat atattcagta cttttaaaga
    10921 gtaatgatta tattactata caacaacttt ctgatgaact atatataagt cgtggaacta
    10981 ttgaaaggga tttagcagaa gttgaaaaac tgctaaaaaa cgaagggatt tccatatata
    11041 agaaacctag taagggaata aagttaaata taagtgaaag agataaaaga caacttactg
    11101 ctaagtttat acataaattt tggagtagaa actggtatat taagcaagag caagaaagct
    11161 tttatcaagc atttgaaaag atacaagcag atgtaaatgg tattttttcg gaagatgatt
    11221 tatcaataat tattgatatt cttaggaatt tcagcagaga aagaaaattt gagttctctg
    11281 attatgaatt tcagtcatta gttattcatt tggcaatagc tgtagagaga attagaaaag
    11341 gcgaatatat agaagaaagt ttgtatactg gtaatataga aaataatcaa atgtcaaata
    11401 ccaattattt agcaaaacaa ttagaagaaa aattttcaat attattacca aaatctgaaa
    11461 ttggatatat aaatttacat ttagtagcgt ctgaccaaca tagagatact aatgaactaa
    11521 aaaatacttc aactaatcct tgtgagacaa gtacaaataa tatatcaaat cttcgggaaa
    11581 ttataaaaaa tacattgtat gagacagctt atgacaagga tttaattgat ggattaactt
    11641 cacatgtaca ttcatcaata aatagactga aatatggact tagtattaaa aatccatatg
    11701 taaatacaat caaacaaaac ttttctttat catttgagca agctttagat ttaaagaaag
    11761 taattgaatt tatatatgat attaggataa atgatgatga atgtgcttat atagcattac
    11821 attttgaagc ttttagagaa agaatgaaag aacttccaca aaatattaga gtaattctag
    11881 tatgcagtac aggattaggt tcatctaggt tattgtctgc aagaataaaa aaatactttc
    11941 caatgataaa gattgaaaaa gtactttccg tacaagaatt agtatccagt aaaattgatg
    12001 cagatttagt aataagtact atttatttag agttagaaac aattccaacc atagtagtta
    12061 gtcctatttt aaatgaaagt gatataagaa ccttggaaag aaatatacaa aatgtttcaa
    12121 gttatacttc taagaagtat aaatctttca gaaacttaat ctttgaagaa aatatattgt
    12181 tgaatattga agcttcaaac tatgaagatg caattaagta tataacagat aacctaataa
    12241 aaaaaggttt tgcaaaagaa ggtatagcta aaagtgcaat tgagagagaa aatttatctt
    12301 acacttcttt ccaatcaata gctacacctc atgctaatcc tagttatatt aaaaaatcta
    12361 atatatctgt tgcaacgtta aaaaatccta ttacatgggg aaatgaaaag gtggaagtta
    12421 ttttctttat ttcattagaa aatgacaaaa acctagagct tgatgaaata tatgaagttt
    12481 tttatgactt cattgacaat aaaaaaaata taaatgacat attaagtgca acttccacaa
    12541 aggaagtgta cgatttgcta gtggaggaga gtatatagtg aaaatatcag atgtattgaa
    12601 aaaagagcaa attgttttta atcttgatac aaatacaaag gaagaaacaa taaaaaaatt
    12661 aagtagtata tttgttgaaa atgggattgt ggacaatgaa gaagaatatg ttaagtctgt
    12721 tttggaaaga gaagaacatt caactacagg tattggtaat ggtattgcta ttcctcatgg
    12781 gaaaagtaat gctgtcaaaa aatcttctat aatatttgca aagactaagc ataagattga
    12841 atgggatgct ttagatgaaa aaccagtaga tagtattttc ttattggcta taagcaatat
    12901 tgataaggat agcaatcatc ttgtactatt atctaaatta gcaactaaac ttatggatga
    12961 tgacaatgta gatgcactaa aattagccaa ttcagaacaa gaaataatca atatatttag
    13021 tgaagaagga gagatgtaga aatgaaaaga aaaattatag cagttacagc atgtgcaaca
    13081 ggagttgcac atacatatat ggcagctcaa gcgttaaaga aagcttcaaa aaagatgggt
    13141 caccttataa aagttgaaac acaaggagct acaggaatcg ataatgagtt aactacaaag
    13201 gatgcacaaa ttgcagaagt agtaatattt gcagtagata ctaaagtcag aaatgctgaa
    13261 cgttttgagg gaaaagaaat actagaagta ccagttaatg cacctataaa ggatgcagag
    13321 aaagtaataa atgatgcact aaagttaatt gacactaaat aaatggaggg aaatgtaatg
    13381 ttgaaaaaga tagtctctga gttaaaaaag catgtcttaa ctggaatctc atatatgatt
    13441 ccattagtaa ttgcaggtgc tatgataatg gcgatatctc gtgtaggtgg ttctttctat
    13501 aatataccag atatatggga tgctaaatat gcagaaagtg ctagtagtat tgttagatta
    13561 ttacatgatt tagatgggtt tggaggtact gcattaggat taatgtttcc tgtaattgct
    13621 gcatttatag gattttctat agctgataaa ttagctattg taccaggtct tgttggaggt
    13681 atgattgcta aagatatagg tgcaggattt ttaggagctt tggcagttgg tcttatagca
    13741 ggatatacat gtttgtttat taaaaatcat gttaaattac caaaatcagc agcttcaata
    13801 gtaccaatat ttatagtacc agtatttggg acattaatca ctgttgtatt aatcaattat
    13861 gtgataggta taccttttgc cacattaaat actggattag aaaattggtt aaatggatta
    13921 tctggtacaa atcagatact aatggcagct attatagggg ctatgatggc agttgactta
    13981 ggtggaccag ttaataaagc agctttaaca acatcacttg cattattaac aagtggaatt
    14041 tatgcgccta acactgctgc aatggtagga atagttatac caccactagg attgggactt
    14101 gctactatac tagcaaaaca aaaatataat aaacagttac gtgaagctgg aaaatcatct
    14161 cttataatgg gtttaattgg agtaagtgaa ggagcaattc cttttgcggt agaatctcca
    14221 ttaaaagtaa taccttcatg tgtaattgga acagcaattg cttcagctat ggcagttggt
    14281 ttaggttcag taaatgcaac tccaattagt ggattttatg gatggtttac agtagagaat
    14341 tggccaatgt atgtgttatc aatagcagtt ggaacagtaa ttgtagctgg attagttata
    14401 gtattgcgtg gaaatccagt gattgaggat gatgaatttg aagatgatga ttttgatgaa
    14461 gcagagtggg aatcttaaaa tttaataact atttaagacg tgttataagc tatattcaat
    14521 atgaatctta tactaaataa agtgttaaac attcagttta gacttaaata taaaagataa
    14581 agaacagagg agaataaatg ataaaagcac atattgttaa tcatacacac tgggatagag
    14641 aatggtattt tacttctgga gatgcattag tacttagtga acaactattt acagatgtaa
    14701 ttgatgaatt agaacgtaat ccagatgtaa gttttgtact tgatggacaa ctttctattt
    14761 tggatgatta tgtacaatta cacaaagaaa aaatagaaat cattaaaaag ttaataaaag
    14821 aagatagact acatattgga ccatggttta ctcaaactga tgcattcttt gctagaggag
    14881 agtctatttt aagaaactta atgattggaa tttttgaaag taaaaagtat ggtaagtata
    14941 tgaaaattgg gtatttgcca gatacatttg gatttaatgc acagatgcct atactattaa
    15001 agcatgtagg tcttgataat atattctttt ggagaggtat acatcttggt aaacaggttc
    15061 aatcaccata ttttaagtgg aaaagcttaa atggagaaag ttatatatat gcagtaaata
    15121 tgccacatgg atatggaact ggtatgcttt tagagccttc attaaagtat gttaatggac
    15181 gcttagaccc agctattgat tttattaaat catatactga tgtagatgag gtgttaatac
    15241 cttctgggaa tgaccagtta aatataatag gtgatttcaa aaataaaatt aaagcgatta
    15301 atgatatcgg aaagtattca tatataacga gttcatatca agagttttta aaatatataa
    15361 agtccataga atcactagaa agctatagag gagaatttag agaacctgta ttagcaagaa
    15421 tacataaatc aattggttct agtagaatgg atataaaatt agcatgtgat aaactagaaa
    15481 acaagctgat taaaagaata gagccattat tagtaattgc taaaaagagt aaaattgaaa
    15541 taagcaatca gttacttata aatacatgga aaaaactttt ggaaggacaa gcacacgata
    15601 gtttagctgg atgtgtaact gatactgtaa cagatgatat tcttcaccgt ataagagaag
    15661 caaatgaaat atgtgacagt attgaaaata ctattgtaaa aaagatttca gaagggctta
    15721 aattatctaa aaatgatatt ttagtattta atacagaagc taagcgtttt aatggatata
    15781 aagagataca agttgtaagt gatagtaaaa atatttattt taaagataat ttagatgcta
    15841 ctataattga agaaacttat gtaaaaccga gagaaaatgt attagaagaa acacctgctg
    15901 gtaatatatt tattgaagaa ccaggatatt atatattaaa agtaagaata aatgtacagc
    15961 ttcctgcact tggttataaa gttgttagtt ttgaatcaag tgataaagaa atggtgtcac
    16021 ttaataaatc taatgatgct tttatatcta atgataatta taagataata tatgaagatg
    16081 gaagcttaaa tttacaactt aaagatggaa cttacatatc aaacttttta acattaaaag
    16141 atagtggtaa tgcaggtgat acctatgact tttctccact aaaaaatgat gaggatataa
    16201 aactttcgtt tgataaagtg tatacagaaa aagctttagg atatgaaaaa atggtggtta
    16261 aaggtactac tttactacca cttacgttag aagatagaaa aaacaaatat cttaatggga
    16321 aattaaatgt aaaagtaagt attgtcttat ccaaagaaaa tccattgatg gatgtaaaac
    16381 tttgtgttga taatacgata tataatcata gactacgtgt tcatataaaa actgatataa
    16441 aagataataa aaacatagca tcattgccat ttggatatat aacaagagaa aatggagttt
    16501 tagacaactg ggaagatatt tacagtgaaa tgcctataga tttagaacct ttggaaagta
    16561 atattactct aacaaaccaa aatagaagct gtactatatt tactaaagga ataaaagagt
    16621 atcaacatat tgaagatgaa attgcattga ccttacttgc tactacagat gaactaggaa
    16681 agcctgattt attatataga ccaggacgtg cttctggtga tacaactaaa aaaggtcaca
    16741 taagaattaa aacagataaa gctcaaatgc ttaagaaatt agaattttca tttgcaatat
    16801 atatggattc taaatcattt gatgaattgg aaattgcgaa tttaacccac aattatctaa
    16861 gggaatgtgt aaattatcaa ttacaggatt acaatttctt tttatatcgt atagataata
    16921 agattcaaaa atcaattgtc aataaaactg taagtagaga attagaaata atatctttac
    16981 cagaaaacta tcttatatca gcatgttatc cttcttatta cagtaaagat aaatttataa
    17041 taagagtgga aaatccaagt agtaataaaa tgatattgga tgagcaaata tttaaaaata
    17101 gaaatgggaa aattgtaaat gcaatagaag atgtagaaga ggaacaggta tatgaaatag
    17161 caccttttga cgttatttca atattattgg atttataagt gtaaaagtaa gtttattgtt
    17221 taaagtctga cagatattaa tctgccagac tttttaattt gtataaattt gggtaaatat
    17281 taatgaatgg ttttagtatt taagtatgaa tttagtattt aatatagaat ttattgaagt
    17341 agaataatta gtttaacagg ataagcagtt taaataaaga attattatat tagag
//
LOCUS       DAMJZE010000081        17258 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJZE010000081 DAMJZE010000000
VERSION     DAMJZE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.081"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(143..511)
                     /locus_tag="PC541_002215"
     CDS             complement(143..511)
                     /locus_tag="PC541_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2839678.1"
                     /translation="MKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(1039..1461)
                     /locus_tag="PC541_002216"
     CDS             complement(1039..1461)
                     /locus_tag="PC541_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2839679.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1939..4842
                     /locus_tag="PC541_002217"
     CDS             1939..4842
                     /locus_tag="PC541_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2839680.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4875..5321
                     /locus_tag="PC541_002218"
     CDS             4875..5321
                     /locus_tag="PC541_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2839681.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            5444..5746
                     /locus_tag="PC541_002219"
     CDS             5444..5746
                     /locus_tag="PC541_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2839682.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5927..7333
                     /locus_tag="PC541_002220"
     CDS             5927..7333
                     /locus_tag="PC541_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2839683.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7677..8735
                     /locus_tag="PC541_002221"
     CDS             7677..8735
                     /locus_tag="PC541_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2839684.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8834..9520
                     /gene="araD"
                     /locus_tag="PC541_002222"
     CDS             8834..9520
                     /gene="araD"
                     /locus_tag="PC541_002222"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2839685.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            9538..10185
                     /locus_tag="PC541_002223"
     CDS             9538..10185
                     /locus_tag="PC541_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2839686.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(10358..11725)
                     /locus_tag="PC541_002224"
     CDS             complement(10358..11725)
                     /locus_tag="PC541_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2839687.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11915..12003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12431..14551
                     /locus_tag="PC541_002225"
     CDS             12431..14551
                     /locus_tag="PC541_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2839688.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14655..15908
                     /locus_tag="PC541_002226"
     CDS             14655..15908
                     /locus_tag="PC541_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2839689.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(16062..17123)
                     /gene="ytvI"
                     /locus_tag="PC541_002227"
     CDS             complement(16062..17123)
                     /gene="ytvI"
                     /locus_tag="PC541_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2839690.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         6789 a         1964 c         2844 g         5661 t
ORIGIN      
        1 ctattaaaag acatataaaa taattaaact taaaagtata tttgcttatc tttttagtaa
       61 ataaaatgaa aaaaagaatt agtggactta tgtaaatatt aaatttaata acttctataa
      121 ataattccat aaaatacgtc tcctattttc ttttttttag ccattgttca agttctttta
      181 tgtcatcatc tgatatttca tttccacaac ctaatgatga tataaagcta tgaatagaat
      241 ttttatgtat aagctctaaa aaatcctttg tttcaaattt tagatattcc tcatgtttaa
      301 ctttaatagt gtataaagta tttctaccat ctttttcaaa ttctaaaaaa cctttatcac
      361 ttaatctaga aagtacttta ccagtagtac cttttgtcca tccatgagaa atttccatat
      421 aattagctac ttctctggct ggtattttat ctatgtcttt atcccatatg aaattcataa
      481 ctaacaactc ggattttggt atttttttca atataatcac tcacctttaa cttgattata
      541 ttagacaaat gtctaatagt caatataata tatcatcatg tataaataat ctttatatta
      601 ttttatgctt gagaactaag tagtatagat ttatagataa aaaagatatt gagagtttta
      661 tggaagaggg gaatatattt tctattagag ataagaatat gacatatatt ttttattgat
      721 ttttacaaaa atactatata gtgaataaat taatatcttt tctaaattag aagaggagaa
      781 tcttattaga taaagattat cctctttatt gaaagtttaa gggatattag agattattaa
      841 tttttctctc tttttttaac taaaagcaat aatacataaa attattattg ctaatgatac
      901 tcttaatgtg gtatatagtc atagtgtcac atgtttatca atagtttttt cattaattat
      961 tgtaacgaat agtaggactc ataaaatact tagaaatatg tttttagata gattttaaat
     1021 atataatcta acttatatct ataaattaac actattattc tccaattcca tttctagttt
     1081 ttttataaat ttattaaatt cttctggttg cctttttcca aacaatactc ttacaagagc
     1141 tgaattgatt tttccaaata tattcctctt gctctcactt tcaataaaaa taagttttgt
     1201 attttctaag tcaatatgta ctaattcata tctagtaata gtactttctc ttttattgct
     1261 tgtttttatc tcatatactc tatttttttc gaaattagtt atttcaacct taaatctttt
     1321 attatttttt tgctgtacaa atgttcctac tgcttttttc tcattaaaat taggaaaatt
     1381 tagtcttgca gattttgtaa atatattaaa tatgtctttt gcagaatatt ttaataaaac
     1441 ttcagtttta aatgtattca tatcgttttc ccttctgtat ataaatttaa tatttataag
     1501 ttaattttta agttaaaata gtataatatg ccatattaaa tcatgttaaa actatttata
     1561 tagctaaaat cttacctata taagtatgta tactatcata taaaaataaa atcttaaaat
     1621 agaaagggtt aaaaacacag tttattataa tttttattgt tgagtagata cgtactatta
     1681 tatatgattg agcaagtttt tcaatcataa atttataaag tattttgcat taaaatttaa
     1741 acaaagaatt aaattattta agattatgaa taactaatac atttagtaaa ttactaaata
     1801 tacaaaatgt aaatacttat caaaatgtat aaatacttat gacatctagt tacttacagg
     1861 gtttaagtag aaaaaaatat caaagtaagt atggcatgat agttgcttat atatttatca
     1921 agaaaggagt tattaagcat ggagttgaga gataaacttg taaacatcat aaatacagaa
     1981 aataaaaaag atccattaac agatgttcaa atagcaaagt ttttaagtac aactagagag
     2041 aacataacta atttgagaaa agaacttaat ataagtaatt caaggcaaag aagatatcct
     2101 tatttaaaat cagcaatttc ggcaatatta caaaagaata agaatatttc tatatcagaa
     2161 attacaaggg aattgatgac agaaggcttt aatatatcga gaagagttgt ggaggaactt
     2221 ttacctaaag aaagtttaga aatggatgta gtagaggaag atgaagaaga atcgaatgac
     2281 ccatttgaaa ctctgatagg taataaaggg agtcttagaa atgctgtaga gcaggctaaa
     2341 tctgctatat tgtatccacc aaaagggtta ccaacgttga ttattgggga aagtggagtt
     2401 ggtaagagtt tattttctag acatatgtat gagtttgcaa agcaaaaaaa gatagtaaaa
     2461 gagagctcca attttgttgt atttaactgt gctgattata gtgacaatcc acaattatta
     2521 ttatctttgt tatttggata taaaaaaggt gcatttacag gtgctgaata tgatacacca
     2581 ggtcttgtag aagaagctga tgaaggagtt ttatttttgg atgaaataca tagattgcct
     2641 ccaaaaggtc aagaaatact attttctatc ttagataggg gaaagtttag gagattaggt
     2701 gaaactaatg ctgaaagaaa agtaagtatt atgttgattg gggcaactac tgaaaatgta
     2761 gagactaatt tattattaac attcagaaga aggataccaa tgctaataac tttgccacca
     2821 ttacatgata gattactaaa agagaaaata gaccttatat acaatatctt tcaacaagag
     2881 tgtaatagaa taaatgcaaa aatatttgta gataaaaatg tgattgaaat acttgcacta
     2941 aaaaagttta gtggaaatat aggacaatta caaaatatga ttcaggttct ttgtgcaaga
     3001 gcttttatga agtttataaa ttccaaagat gaagatagtg aatctatagt agtagtagat
     3061 ataaatgaag ttttaaagct taaagattcg tttaaagatg ctgcctttca agagattgaa
     3121 tatacagaga taagaaaata tttgaaaaat gctatattta taccatttaa tttagaagaa
     3181 tcatcagtaa aaggaagtct tttaagtgat gaatatatat taccagagga catatacaaa
     3241 acaattgaaa agaaatatta tgacctaaaa tccctagaca ttgaagatat agatatagag
     3301 aatattttat ggacttttat tttaaataga tttgctgact taaagtttaa tcttgatagt
     3361 aatgaagaaa ttttttcaat gagtgattta aatagttttg taagtgatag tttagttaaa
     3421 ctggtaaaag aacttatgaa tgatatagtt gaaaaaaata tgaatttaga agtaaataaa
     3481 aatatgttca aatatctagc aatacactta gaagaagcta taaaaagaat taagctaaat
     3541 caaaagataa taaatgtaaa tttagaaaaa ataaagattg acttttcaaa agaatatgaa
     3601 attagtaaaa gttttgcaat aaaaattgag gaatcaattg gtattaagat ttcagatgat
     3661 gaaattggat ttataacgct ctacataaag tctgctctaa aaaatgaagt gaaaaaagat
     3721 aaagtagggt taataataat ttctcatgga cgtatagcta cagagactgt aaatgtagtt
     3781 aaagaacttt taggtgtaaa gtttcctgtg gctatagata tgcctttaga tgaaaaacca
     3841 ataaatatat acaataaggc agtagaattg tcaaaaataa tagaccaagg gaaaggcata
     3901 ttgtttttgg tggatattgg ttcacttaca aacataggtc aaatagtaaa taagagaacc
     3961 aatataagta ctaaaactat tgatagagta gacctcctaa tggcactaga agcaactaga
     4021 aaagtttcta tgggagagga agaacttgat gaaatatttt tttctttgct aaaagatagg
     4081 atgggatata actatgaatt agaaaaacac atagataaat caaatgcaat tataacatta
     4141 tgtcttactg gagaaggaac tgctaagtat ataagtaaaa cgttggaaga aaagtatgat
     4201 aacactaaat gttatcaaat gagtgcattg gatgaaagtt tattttgtaa aattgaaaat
     4261 ctaaaagaga caaacaatat attagctatt gttgggacaa taaaccctaa gataccaggt
     4321 ataaatttta taccctataa taaagaactt ttcaaaaatt tggatatcta tctatcaaaa
     4381 tcaaaacaac aagatgacag accaattaaa tcgtatgaaa gaatgcttga tgaggattta
     4441 gtgatttttg aaccagatat atattttaaa aaggatttat tagaatatgt atgttcaatt
     4501 cttataaata aagaatatgt agaaaaagat tacttagatt ctgttttaca tagagaagaa
     4561 atgcttccaa catattctaa aggagctata ggtgtgcctc atggagattc ttctactgta
     4621 aatagtacaa ggtttgtttt tgttaaacta aagaatccta ttgattgggg tgtaggtaat
     4681 gtaaatttca tatttatgcc agtttttcag gctaatgata aagagattgt taaaaatgtt
     4741 cttgagatac taaaagatgc agaatttatg aacaatgcca ataagtgttt tgataaagat
     4801 gcttttagaa ggattatatt tgataaattt aaacatctgt agccatagca aaaaaattga
     4861 gaggagttag agtaatgaat gtagatttaa tatcattggg tttagaatct aatgattatg
     4921 aaagtgttat tgaagaatta ggaagtataa tgtgtaaaaa agaatatgta aaagaaacat
     4981 acataaatgc tgtcttggaa agagaaagga cattaccaac aggtctagat attggagaca
     5041 tgtgtgttgc tattccacat acagattcaa agcatgtaaa tgagtctaat gtagctgtag
     5101 ggatattgaa aaatcctgta aagtttaatt caatgataga cccaaaagat agacttgatg
     5161 tagaacttgt atttttatta gcagtaaaga acccagactc acaagtaaaa ctgttaaaag
     5221 atttaatgtc agtttttcaa aatataaagt tattaaaaaa tataaaaaac gcatcaacaa
     5281 aagaagaagt agctaaatta ttggatttta tagaaattta atgtaggaga attttataaa
     5341 taaaaaatta aaatttaaac aatcaaagta agtattattt ttttaatagg tttaatctta
     5401 tataaaataa aaatttagag tgaaaaatta ggaggaaatt attatgaaac aatttaatgt
     5461 attatcagtt tgtggttcag ggacagttac atcatctatg gtagcaggta aattaaaaga
     5521 agtactaggt aataagggga tttctattac tacaacagaa gctagaccaa cagaagcact
     5581 aaatttagca caatcaggaa gatttgactt tataacattt acgagtccac ttcaaacagg
     5641 cgattatgga ataccaacta taaatgcatt tgcttgccta actggtatag gggaagaggc
     5701 attctttgaa gaagttttga ctgttattga agggatagat aagtaagttt tggagacgtg
     5761 aatttatagt atttaaatca atgatttaga tactataata agctaatatt aactaaatat
     5821 ttgtggaaaa aacattttat atgttgtcat aaattaaaaa attataaaaa tagtcactta
     5881 agtattaaat taagcttata aatatttata agtaggaggg aaaaagatgt tagaatttat
     5941 gaaatctata atcgatacat ttggttctgc aattatagtt ccaattataa tattcataat
     6001 cgcaaagata tttaaagtaa ctactaagaa atcgtttctt tctgctgtgt atgcaggagt
     6061 tgctttacaa ggttttacac tgattttaaa ttcatttaca ccagtaataa caccagttat
     6121 caataggatg gttgagagta caggagtaaa cctaccagtt tttgatgttg gttggcaagc
     6181 aacatcattg gttgcatttt caacaagtgc aggtatgata tatttaggtc ttggtatatt
     6241 attacaaaca attttatttt tgataaaatg gacagatgtg tttcaaccat cagatttatg
     6301 gaataactat tcttatatgg tatggggagc aatggttata ggagtaacag gaaattttcc
     6361 tttaggtata gcatgtatgg ttttattaaa cctatatagt ttattaatat cagagttagt
     6421 tgcaaagaga tggtctagtt actatagata tcctaattgt acaataatag ctatgcataa
     6481 tgttgaggct tctgtgtttg cagtatttgc agaccctata tacaataaat tgggattaaa
     6541 taaaataaaa ttaaatccaa aagaattaga gaagaaatta gggtttttag gtgaaccaat
     6601 tactttaggt ctttttttag gtatgtttat aggtatatta ggtaatatga cgagaataaa
     6661 tactatggaa gcttggggag aaataatgaa agtaggtata tctacaagtg cagtgatggc
     6721 aattttccct aaagtagcaa gtatgtttgc tcaagcattt gcacctataa cagaagcagc
     6781 tagaaagatt atgcaaaaag ctggaaaccg tgagtggtac atagcagtaa atgatgctgt
     6841 tggatatggt gaacctgcaa ctttaatatc aggtcttata ttaattccaa taatgcttgt
     6901 aatagctatg gtacttcctg gtaataaggt attaccagtt gtggatttat tggcaattcc
     6961 atatatggtt caaggtttag ttgccataca taatggtaat ataccaaaag tattagtttc
     7021 aggtataatt tggtttggat taggattata tgtttgtaca tctacagcac cattatttac
     7081 agatatggct acaaatatag gtgtagctat acctgcagga gcaatgttaa taacaagttt
     7141 taatatacta ggaaaacctt tgatgggatt agttttcttt gcatttctaa gtgcaaatcc
     7201 aatttatata ggtctttcag ttgtaattta ctttgttcta tgggcattgt ttagaaagaa
     7261 caaaacaagt attcttgatt atttagaaaa acaagcactt aaaaatgtag aagaagagcc
     7321 tgtagcagtt taagtttata aaaatgaaat aaaaataatt aaaggagttt ataattgatt
     7381 ttatataagt atcagataat acctgagaaa tatttaaatg aaatatagac ttctttttat
     7441 ttgataatat ttgtaattat aatgcattgt atttttatag ttgagttttc tgtataacct
     7501 gctatttaaa acatactctt ttatatttat acagtaatgt ggtttaattt tataaaatgt
     7561 aatctctact ggtaaaagac tttgtatata aggcatttgc ctgaataaac catttatctt
     7621 ttgtatggca tagatattgc tatatagaag tatatagcat gaaaggagat atttatatgg
     7681 gaaataaaat gagagcatca gtattgtata atgtaggtga tgtcagatat gaaatggtaa
     7741 atataccaga aataacagat acgcaagtgc ttgtaaatgt aaaatatgtt gggatatgtg
     7801 gttcagattt acctaggtct atggtaagtg gtttgagtgg aaatacaaaa tatccattga
     7861 ttttagggca tgaattttct ggtgaagttg ttaaaattgg agaaaaagta aagcatatta
     7921 atgtaggaga tagagtagct gttgcaccat tagtaccttg tggaaaatgt gattattgta
     7981 atgaaggtaa ttttggcttg tgtgatgatt acaatataat tggaacaaga gtaaatggag
     8041 cgtttgcaga atatgtaaga gttccagaag aacatatttt aaaactacca gatacattgg
     8101 attatgaaac tgctgccgga attgaaccag caactatagc ttatcatggg atttcaaagt
     8161 ctaatattag agtaggagat agtgtagttg tgttaggatg tggtccaata ggtcagtttg
     8221 ttatacaatg ggcaaaggtg tttggtgcat ctaagattat agcagttgat atttttgatg
     8281 aaaaattaga actttctaaa cttctaggag caaactatat acttaactca aaagaagtaa
     8341 atgtaatcaa agaaataaaa aaaataacaa atggtggtgc tgatgttgta atagaaactg
     8401 ctggtagccg atttacacaa gaacaatctc tatttgtagc aaaaaagaga ggaaatatag
     8461 tgtttgtagg tatatcacac acagaattac cacttagtgc agacgctact gagtgtatat
     8521 tacgtggaga acttaccttg aaaggttctt ggaattcata tacaagccct tatcctggaa
     8581 gagcatggac tgcaacattg gattttatgg aaaaaggtga cattattttt aaacccatga
     8641 tttcagataa aataggtctt aatgaagtgg gtgacttttt aagtaaaatg agcaagagag
     8701 aaattaattt caataaaatc ctagtagaaa tataaaaatt taataaaaaa ttctaaaagc
     8761 aattaaattt tataactatg acattaaata aaataaaaat aattttctta aataaaatat
     8821 aggaggaaga aaagtgttag aaaatttaaa agaagaagta ttaaaagcaa acttagaatt
     8881 acctaagaaa aagctaatta cttatacttg gggaaatgta agtgggatag atagagaaaa
     8941 aggattagtg gtgataaagc caagtggtgt agagtatagt aatatgactg tagaagatat
     9001 ggttgtagtt gatttagagg gaaatatagt tgatggcaaa ttaagacctt cttcagatac
     9061 cccgactcat ctagttttat ataaagagtt tgaagaattg gggggaatag tacatacaca
     9121 ttcaagttgg gcaacaattt gggctcaaat aggaaaatct ataccatctt gtggtactac
     9181 tcatgcagat tatttttatg gaagtatacc atgtaccaga aaaatgacta gagaagaaat
     9241 ttgcaatgaa tacgaaaagg aaactggaaa tgtaatcatt gaagaattta tgggaagaaa
     9301 tccaattcat tgtccaggaa taattgtaaa tgaccacgga ccttttacat ggggaaaaga
     9361 tgcaaatgag gctgtacaca atgcagtagt tttagaagaa gtcgcaaaga tggcttatta
     9421 tacagagtta atgagtcctg ataatattat ggataaggtt cttatgaata agcatttttc
     9481 aagaaaacat ggaaaaaatg catactatgg tcaaaaatga ttatctggag gtgaattatg
     9541 ctagactctt taaaaggtaa agttgtggaa atagcaatta aagcagagca ttatggttta
     9601 tgtaaagaaa agactggtaa ttttagtatt agagataagg atactggata tatacttata
     9661 accccatcag gtgtaggaag agaagaatta aaagttgaac atatttgtgt attggatttg
     9721 gatggaaatg taatagaagt tgagaaaggt attaagccaa gtagtgaggt attgatgcat
     9781 attgaaatat acaaaacaag agaaaatata aatgccatat cacatactca ttctttatat
     9841 gcaacagcat tttcagtagc aaagaaacca attataccaa ttgtatatga atcagttaat
     9901 tatggcggtt atgtagatat tgcttcatac gaaagaccag gaactttaaa attagctaag
     9961 agtgtttcaa aattactgat agatacagat gcatgcttat tagaaagaca tgggttgaca
    10021 actgtaggta atgatttaga cgaagcatta ttaaaatcaa gatatgtgga agaggtagca
    10081 gaaatatact ataggtcttt agtattaaat caatttaaag aacttgaagt agtagatgtt
    10141 gatgagttta aatcttggaa atacccagaa gaaataaagt tttaatctaa ataattttca
    10201 ttgaagtggt taattaaaat atatgtatta tttatatcaa ttactatgtt ttattatgtt
    10261 aattaccgta ttttgcaaat atatttatta cattttttca aaaaaatata gaatccgaaa
    10321 gccatataaa attgacttgg gattctatac tttttattta tgcatttttc tttatctgat
    10381 tttctggaga atttatatgt tcaaaatatt ttttagtttc agatactatt ataggtgata
    10441 atgcaagtaa agctataagg ttaggtattg ccataagccc atttacaata tcagcaatta
    10501 tccaaacaac ttctaacttt aagaaagcac ctaataacac catagccaca aatacagttc
    10561 tatacccatt catgcctttt acaccaaaca gaaattcaaa acatctttct ccataataac
    10621 tccatcccaa aattgtagta aatgcaaata atgttaaact aactgtaagt aatattggtc
    10681 caaatgtagg aattgctccg ttaaaagctg cttgtgtcat aacagctcca tttaggtctg
    10741 aacaccacac tcctgaaata acaagagaga atccagtaag agtacagatt ataatagtat
    10801 ctataaaagt acctgtcata gatataagac cttgttccgc tggccattca gttttagcag
    10861 cagctgcagc tattggagca ctacctagac cagactcatt tgaaaataca ccacgagcga
    10921 taccatttct aattgccagt gacatagttg ctcctaaaaa accaccagta gcagcagtag
    10981 gtgtaaatgc acttttaatt attaacataa aagtttcagg gattttattc caaaaagata
    11041 ttaaaatgat tccacagatt acaacatata taactgccat aaaaggtact attttagtag
    11101 ctacattaga tatactttta agtcctccaa atacaactaa agctacaaat actgctaata
    11161 caacccctgt aattctaggg tctatcccga aactattatt tattgcatct gttattgaat
    11221 ttacttgagt aaaagtaccg attcctaaaa gtgcaactag tataccactt atagcgaaaa
    11281 atattgcaag aggtctccat ttttctccca taccatttac tatgtaatac attggcccac
    11341 cagacacttg accattttta tctttagttc tgtattttat tgcaagaaca ccttctgaat
    11401 acttagtagc cattccaaag aatgctgcta tccacatcca aaaaagtgca ccaggtccac
    11461 cagctttgat agctgtagca acaccaacaa tattaccagt accaactgta gcagctagag
    11521 cagtacataa ggctgcaaaa cttgaaacat caccactacc tttgttttca gcagagaata
    11581 ttaacttaag agctcttggc agtttagtta tttgaacaac tccaagctta acagtaagta
    11641 gtataccagt accaactaat agtaccagta ggggtggtcc ccaaataaaa gcatcaactt
    11701 tgtttaaaat atcaattaga ttcataaaaa cactccctca ttttttttgc tttacataat
    11761 caatagaatt tttaatgaag taattgacag gaaataagta taatttatta ataatttcta
    11821 gtgtaataaa aaaagctaaa tctaaaaaag attcagctct ggatgtacac taattagcaa
    11881 ccaaaataaa agtattagta aatactgttt ttggtagata atccctgtcc ttttgcctga
    11941 gagattgagc ataaaagcct tgccccttcg gcgcccataa tgggtctctc cagagttccg
    12001 taaattttac agtcctactt ttaaaagtac ctgagagtgt tactccttcg gtgtgctata
    12061 tagcaactct tctataaaat cttaaccgga tattgatttg tcatagctta ttgtattaat
    12121 aatattaata taagttttta tgttttgtca actaaaaaat taaaaaacaa agaaaaaata
    12181 taaaaatgta agcctattta cgaattataa acttagaaag atgagtaata tacgtgaaaa
    12241 tgaattttgt aatacacata caaatgaagt gtaaaataag ttttaaataa aaaaataata
    12301 aaatcttaaa taattaatta gatatgatta aattatggta atattatagt tacgatatta
    12361 agaaaagttc tatttagtta gattttaagc gtaatatgag attatgtata gagaaaggat
    12421 gtatttttag atgggaaaaa tattagtgct agcagaaaaa ccttctgttg gaagagatat
    12481 agcaaaagtt ttagggtcaa aaaatgaaaa aaatggttat atagaggggc caaagtatgt
    12541 gataacttgg gcactaggtc atttggttac acttgctgac cccgaaagtt atggagaaag
    12601 atataaatca tggagcttag aagacctgcc aatacttccc aagcacctta agactgttgt
    12661 aataaaaaag agtggaaagc agtttaatac agttaaatcg caaatgaata gaaatgatat
    12721 agatgaaata gttatagcaa cagatgcagg tcgtgaagga gaacttgtag caaggtggat
    12781 aatagaaaaa tcacaggtaa aaaaacctat aaaacgtctt tggatttctt caagtacaga
    12841 caaagcaata aaagacggat ttgcaaaatt aagaagtgga aaagaatatg aaaatttgta
    12901 ttactcagca atagcacgag cagaagctga ttggattatt ggaataaatg caaccagagc
    12961 tttgacaaca aagtacaatg cacagctttc ttgtggaaga gtacaaactc ctactcttgc
    13021 aatgttgctt aaaagagaag aagaaataag aaatttcaaa cctaaagagt attatggatt
    13081 agaattaatt gctacaaagg gaaattcaga tataaaattt atttggaatg ataaaaacaa
    13141 taattcttca acttttagta aagaaaagat agaatccaca ttaaaaaaag taaaaggtgt
    13201 agacataaaa atatctgaag taaacaagtc atataagaaa aaatattcac ctgcactcta
    13261 tgatttaaca gaattacaaa gagatgcaaa taaaatgttt ggattttcag caaaagaaac
    13321 tttatctata atgcaaaaac tgtatgaaca tcataaagta cttacttatc ctagaactga
    13381 ttctagatat ttaacatcag atattgttgg aacattaaaa gatagaataa aagctgtaaa
    13441 tattagtgat tattcaaaga tttgtaatag attattaaaa agcaatatta tagctaataa
    13501 atcgtttgtt gacaattcaa aggtaacaga ccaccatgca ataataccaa cagaacagaa
    13561 ggtattcatg agtgatttta gtgataaaga gcgaaaaata tataatcttg tagtaaaaag
    13621 atttttagca gtactatctc ctccttttga atatgagcag acaactataa gtgcaactgt
    13681 agaaggagaa atatttaagg ctaagggaaa caaagtaata aatttaggtt ggaaagaaag
    13741 ttataaagat attgaagatg aagatgagta tgaaagtata ccaaatgttt tagaaaaaga
    13801 tgtacttaag gtcaagggat taaagataac ttcaggaaaa acaaatccac caccatactt
    13861 aaatgaggga acattattga ctgctatgga aaagaatggt ctagggacag ttgctacaag
    13921 agcagatata atagaaaaat tattcaattc atttttaatg gaaaagaaaa ataaagatat
    13981 atatataaca tcaaaaggaa gacaattatt agatttagtt ccagtagact taaagacacc
    14041 agaacttaca gcttcatggg aaaagaaatt attggatatt tcaaagggaa aacttagtaa
    14101 aaatacattc ataaatgaca tgaaagatta ttcaaaagat attgtactag aagtaaaaaa
    14161 tagtgataat aaatttaaac atgataactt aactaaaaat agatgtccag aatgtggtaa
    14221 atttatgtta gaggtaaatg gtaaaaaagg taagatgttg atttgtgaag atagagagtg
    14281 taatactaga aaaactatat cacaagtaac caattcaaga tgtcctaact gccataaaaa
    14341 attagaactt cgtggagaag gtgaggggag actcttcact tgtagttgcg gatacagaga
    14401 aaagttgtct tctttcaaca agagaaagca agaagaaaaa cagaaggcat caaaaaaaga
    14461 tgttaatcaa tatttaaaaa atcaaaataa aaatcaagag acatttaata atccatttgc
    14521 agaagcatta gctaagctaa aaaacaaata attttaaagt tattattata cacaattata
    14581 actttaactg tatattagtt atatgtactg atataatata gtgtatagtt taggcatgaa
    14641 agtagggctg ttaaatggat ttaagtggca tatttaaata ctattgtaaa gagtgtgaaa
    14701 atacatggaa taattcgagt gttgaattat ttgagaatat agaaacgtat agtaaagatt
    14761 cacaaaaaaa gagggaaaaa gaattagata aattgctaaa tacaatatca gttcatttag
    14821 agaggtatcc aagtgatgct gtattgagaa aaatgtgggt aaaaaagggc gaggttttct
    14881 tacaaaagac attggaaaaa gaaaatattt ttaagttaga aaaaatggat gtagaggata
    14941 gaaaaaaatt tttagatata acaaaacagt ttattagaga tgctagaaaa tttgatgatg
    15001 atttacctat aggtgatatt atgcaagcta tgaggaatgt atggatttca aatgcattac
    15061 aattattatt tggtaaagaa gtatattatt caaaagctaa ctttgcatat agtatgttat
    15121 atccatatac tgataattat ttagacaata caaatataga taaaaatgat aagattttat
    15181 ttaataactg gttagaaaaa aggctcctgg gagaacacat taaatctaag gattatcatg
    15241 aaagtaaagt atctcaaatg atagattata ttgaaagtgt ataccctaga gaaaagttta
    15301 cagaagttta tgaatcgtta ttattaatat ttaaaagtca agtaaatagt ttaaaacaac
    15361 atggtaagga aaatcatttg tgtaaagaag atttattatc catttctatt gaaaaaggag
    15421 gttcatccgt tttagtagat ggatatttaa taagtggatt gatgacaaag gaagaaatag
    15481 agttttgtat aggatatgga tttttattac aaatatctga tgatttacaa gatataaaag
    15541 aagatttaaa atacaaccat aaaactatta ttacagagat gtcaaaagag ggtactttag
    15601 ataaagttgt aaataaacta ataaatttta ctattgagtt aatagatagt tttaaaatta
    15661 ataataaaaa taaatctgta atagctatga taaagaatga ttgcttaatg ttaattttat
    15721 tttctgtagt ttataatgct gaattttttt ctgtaggata tataaaagaa gtagagaaat
    15781 ttattccata tacaatagat tattcattag agattgaaga aaaaataaaa gaaaaattta
    15841 aaaatataga tgttttaaat aatgaaaatg aatataaaga aatgattgat attatttgtg
    15901 cagagtagaa aaaaagggag tgtcttagaa tagacttttt agtttatgag gcactctttt
    15961 tgtataaaat caaatgtatt ttcatcattt taacaattaa aaagagctta tccctatttg
    16021 agaaagccct ttttaattta aataaaaatg tcaaggttaa actatttaaa tgcagtaata
    16081 atcccattat catgtaagtt ttttatgatt gtagctgcga tagggaatcc aaataatcca
    16141 gctattccaa atagttgagc tccaacaaac atacacatta atgttattaa aggatatagg
    16201 ccaatttggt gtcctactac ctttggttct aatatttgtc taacaacggt tataattatg
    16261 tatagtatta aaagtcctat tgctaaatta atatttccat taatgagtga gataatcatc
    16321 caaggtgtca aaatacttcc tgtacccaag actggcaaaa tatcaacaag tgctatgatg
    16381 acagatatag taaatgcatt ttcaaccttt aggatagtaa gtccaataag tagttctatg
    16441 aatgtcactg atagtagtat tgcataggct tttagtagtt ttaaaagagc attaatactg
    16501 ctatttttta tatttattat cataaattga cttttttctg gaaattgttt taagataaag
    16561 tttactattt tttgatagtc aaaagtaaag aaaaaagaag caattaaagt aataataagt
    16621 tttaacataa atgcaggtgt tttacttgca atactagcaa tagcattgag tgcatttgtt
    16681 gaaatagaag ctactaaaga catcatagat tgacttatat tatcccaacc aagtattaat
    16741 gctaggttaa cattaggtgt actagcatct atctttgaaa ataaagtatt tagcgttggt
    16801 tgtatacttg atttatatat ttcaggaagt ctaaaaaata catcacttac acttgcaaat
    16861 atcttcacac caaagctaac taataaaaat atacacaacc cataaaaaat taaaagtatt
    16921 actatagaaa taagtgtacg atttaaattt gtattatttg taataagtct tataataggt
    16981 cttaagatgg atgcaatgat aaaactgatt acaaaaggca ttaataatgg caataagaat
    17041 tttaatacaa tatatacaat acttgttatt aaaactacat atgtaaattt aattataaaa
    17101 ttcttttgct tttgaatatc cataatataa aacctccaat gtatttgata ttattctaaa
    17161 tatggtcttt tttacagaaa atatactact gtatattgat tataaactat aaagcttatg
    17221 taaagtaaat aaattatgta aaatttaggt taattttg
//
LOCUS       DAMJZE010000082        17093 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJZE010000082 DAMJZE010000000
VERSION     DAMJZE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.083"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(187..711)
                     /locus_tag="PC541_002228"
     CDS             complement(187..711)
                     /locus_tag="PC541_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2839691.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(776..1528)
                     /locus_tag="PC541_002229"
     CDS             complement(776..1528)
                     /locus_tag="PC541_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2839692.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1559..2857)
                     /locus_tag="PC541_002230"
     CDS             complement(1559..2857)
                     /locus_tag="PC541_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2839693.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2874..5138)
                     /locus_tag="PC541_002231"
     CDS             complement(2874..5138)
                     /locus_tag="PC541_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2839694.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5393..6211)
                     /locus_tag="PC541_002232"
     CDS             complement(5393..6211)
                     /locus_tag="PC541_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2839695.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6204..6902)
                     /gene="cbiQ"
                     /locus_tag="PC541_002233"
     CDS             complement(6204..6902)
                     /gene="cbiQ"
                     /locus_tag="PC541_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2839696.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6905..7189)
                     /locus_tag="PC541_002234"
     CDS             complement(6905..7189)
                     /locus_tag="PC541_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2839697.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7179..7931)
                     /locus_tag="PC541_002235"
     CDS             complement(7179..7931)
                     /locus_tag="PC541_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2839698.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8113..8305)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8437..10209)
                     /locus_tag="PC541_002236"
     CDS             complement(8437..10209)
                     /locus_tag="PC541_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2839699.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10223..11152)
                     /locus_tag="PC541_002237"
     CDS             complement(10223..11152)
                     /locus_tag="PC541_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2839700.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11373..11558
                     /locus_tag="PC541_002238"
     CDS             11373..11558
                     /locus_tag="PC541_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2839701.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11551..12252
                     /locus_tag="PC541_002239"
     CDS             11551..12252
                     /locus_tag="PC541_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839702.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12254..13177
                     /locus_tag="PC541_002240"
     CDS             12254..13177
                     /locus_tag="PC541_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2839703.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13334..14257
                     /locus_tag="PC541_002241"
     CDS             13334..14257
                     /locus_tag="PC541_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839704.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14250..14987
                     /locus_tag="PC541_002242"
     CDS             14250..14987
                     /locus_tag="PC541_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839705.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            14998..15771
                     /locus_tag="PC541_002243"
     CDS             14998..15771
                     /locus_tag="PC541_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839706.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16312..16920)
                     /locus_tag="PC541_002244"
     CDS             complement(16312..16920)
                     /locus_tag="PC541_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2839707.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5514 a         2957 c         2156 g         6466 t
ORIGIN      
        1 aattttttta ataaaacttt attttttagt taaaatcata caatatatag atttccacaa
       61 tacaaataaa ttaaaaagtg aaatgtattt gtctaaaaac ataataacat ttcactttat
      121 cctcattttt aagtaatata tatttttaat ttttatttat aactaaatct agtttttata
      181 taaattttag tttttttcta catctgagta atctattcca aaagtttcta cagtaacaga
      241 tttcataact tgtggagttt gtggcttatc agctgaatct gttctaactg atgctatttc
      301 atcaactgta tccatacctt ctatcactct accaaaacct gcatactgac catctaagtg
      361 tggtgagttt ttatgcatta tgaaaaattg agaaccagct gaattaggtg ccatagttct
      421 agccattgat atgactcctc tttcatgttt taaattgtta gtaaatccat taccactaaa
      481 ttcacctttt attgagtgac ctggaccacc cattcctgta ccatttgggc atcctccttg
      541 tatcataaat cctggtatta ctctatgaaa tattattcca ttgtaatatc cttcatttac
      601 taatgataca aagtttttaa ctgtatttgg agctatattt ggatataact caattttgat
      661 ttcttttcca ttttccattt ctatagttac tataggattt ttattttcca taatttatta
      721 ccttcctttt cattagttaa attacataat tacatttatt atattatact ctaaattaaa
      781 gtctatcaag tatatttttt cctatttcat atccttcttg ataaatagtt ttacaaaact
      841 catcatcaaa gttaagcgtg tctttaggag caaattgagt attaggtcta actactatta
      901 ttttatcttc atcatatact tttttaatat cttctggcaa ctcataatta tattttgtgg
      961 atattaaaat aattttatcc acattatccc caattaatgg ttcaattaat gcactcctaa
     1021 ttaaacctcc atctaatttg aaaccatcat aaattccttc ttgcacatct ttcacaaatt
     1081 gttctttaaa tccaagagtt gcgtttggat ttgatggtaa caacccactg tatgaaatgc
     1141 tgataatggc ttcattacga ggcatttttg atacgtcaac aatcttttct gatactactt
     1201 tattatcaac ttcaacataa ttaacataaa agttcatttc ttctatatta ctatcatcta
     1261 gttcccttag gttgtctata gcaggagaat tatccacagt attatttact atttttacat
     1321 ttccattctg gatatctttt atatttgtcc acattttctc caagttatca acattccctg
     1381 tgtacaaata atatccattt attgctccta ttgatgtccc tgaaatcgaa taaaatttat
     1441 ttattcctcc atcatacaag gctttaacta ctcctgcttg ataagcccct tttgctcctc
     1501 caccttctaa acataatcct aatttcatag tctcatctcc aaattgttta tcttatattt
     1561 atatttcaaa aaacttacta aatgttttat atatactata gtggtcctca acatttccta
     1621 attctcttat agaatgcatt gctaaaattg gacttcccac atccacagat ggtatatcta
     1681 tatgagtaga agatattgga cctatagtac ttcctcctct ttcatctgac ctattaacaa
     1741 attcttgata ctcaacatta gcttctctac atatattttt ataaatagct atagaatatc
     1801 catcactagt atatgcctga ctagcattta tctttataac cggacctttt cccattattg
     1861 gtttattagt tgggtcatgc ttttctggaa tgtttggatg aatagcatga gctaaatctg
     1921 atgatatcat aaaagatgaa taaatagact caaagaatcc ctctctatcc ttacccattg
     1981 atatgcaaat tctctccaat atatttaaca acatgtttga atctgcacct tgtttagtag
     2041 agctaccaac ttcttcattg tcaaatacag ccattacatt tacaccatgt tgacctttag
     2101 tattaacaag agcattcaaa ctagcatgta ccattgcaag attatcttgt ctcccaatag
     2161 atataaattc atcattagcc cctattaaac ttcctttttc aaattcatat aaaaataaat
     2221 caaaatctat aatatcgtcc atatttacat ctaagttaga tgctatttgc tttataagaa
     2281 aattatcttt ttctaatgta tcatttaaca tcccaactaa aggtaacata tctttttgct
     2341 tattatatga gtgaccatca tttatacttc tatttaaatg tattgctaga tttggtatta
     2401 tacaaattgg ctttttaaag tctataagcc tttcatttgg ttttaatata ctatcacctt
     2461 ttaaaactac tctaccagct atcccaagag gtctatccaa ccatgtactt aatattgctc
     2521 ctccatagca ttctgtattt aacttcaaat aagatttttc agcttctatt tctgcatttg
     2581 gtttaatact aaatgtagga gaatctgaat gagagcctat tattctaaat ccatctcttt
     2641 ctataccttc tgaatttact acaaaagcta ctatagctga taaattctta gtaacataat
     2701 actttccacc aactcttaga ttccatcttt gattcaaaac taattcttca aatccatttt
     2761 tatctaagat ttccttagta ctctcgactg catgaaaaga cgacggacta tcatatatga
     2821 agtctattaa gtttttagca aagtttatac tatccattcc tcatttacct ccattataaa
     2881 atatttagtg ttgtatattc tttatttatt tttttaattc cctgttggtc aaaagctata
     2941 gtaacatctg taccaattat agaaaccact gtcccaaccc caaatttagg atgatgtacc
     3001 ttagaaccaa gttttatatc atctggatta gtttctttat tagaatcttt tattgtagca
     3061 ttaactttat ctgcaacttt agatttgctt atagtactca tatacttttt agtatattta
     3121 tctaacatat tataacttgc tttagaatag cttagttctt tttttacttt gtacaatctt
     3181 tctatacatt cttctggcag ttcttccata aatcttgaag ctattgcaac atttgtttta
     3241 ccatataatg ttctcttctc tgtcaatgac atatatagct cttctttagc tctagttata
     3301 ccaacataac aaagtcttct ctcttcttct atttgtgaat cactcataga ttttatagct
     3361 cttgatatag gaaataaccc ttcttccata cctgttaaaa acacaacagg gaactctaaa
     3421 cctttggatg tatgaattgt cataagcgaa accctatcat tgtcttcttc atcatttgat
     3481 tcagatgtaa gtgctatact tgttaggaat gtctctaagt ctttattttc ataagtatca
     3541 tcattactac tttcaaactc caaagctata gaaataaatt ctctcaaatt atcaattctg
     3601 tcttgagctt cttcaccttt gcctgttaaa tctttttcat ttttatttct tatctctaca
     3661 agctcgtcca tatatcctgt agtatccaag actttttcta taagtttgct aacaggataa
     3721 acttctttta tagttctaag agtccctata atatcaacaa atccacttat acttgccctt
     3781 gccttagtag atatatctga atttgtttct atatcaatta atactgagta tatactttcc
     3841 tgttttaagt tagctctatc ttcaattttt tctatagttc taagccctat acctcttcta
     3901 ggaacattta taatcctttt tatagatata tcatcttgtg gattttgaat aactcttaaa
     3961 tacgccaaca aatcctttat ttcttttctc tcatagaatt ttgtaccacc atatatattg
     4021 tatggaattt gacttctatt taaagcgtcc tctacaggtc ttgcctgtgc atttgctcta
     4081 taaagtactg caaaatcctt ataaggtcta ttttcttttc tagctatttg tgcaattgaa
     4141 tcagcaatga aatcagcctc ttctatttca ctaccagtta attgtatctt tataagttct
     4201 ccttcttttt tttcactcca aagctttttt ctttttcttt ctatattatt agatataact
     4261 ttattagctg catctaatat aacttgtgta gacctataat tttgttccaa ctttacaaca
     4321 tgtacattgt cataatcttt ctcaaattca agtatgtttc ttatatctgc acctctccat
     4381 ccataaatac tttggtcatc atctccaaca acacaaatat tttgatgttc tcttgctaat
     4441 aattttatca attcatattg agctttactt gtatcttggt actcatctac cattatgtat
     4501 ctaaatctac ttctgtaata tgctaacact tcatcattag ccttgaaaag ttctacagtt
     4561 ttaagtatta aatcatcaaa gtcaagtgca ctatttcttt ttaaacggtc ttgatatagc
     4621 gcataaatat ctgcaatctt aaccattcta ttatctgcca tattgatatc tttaaactgc
     4681 tttggagtat aaagtttgtc ttttgctcct gaaattgcac ttataactgc ttttggttca
     4741 aacactttat cacttaaatt taattctttt agacagtctt taaccaatgt aacttggtct
     4801 gctgaatcat aaattacaaa acttctattg taaccaatct tatttatatc ttttcttagt
     4861 atacgaacac agcaagaatg gaatgtactt atccacatat ccttagtttc aggacctata
     4921 ttttgttcaa ctctctctct catttcattt gcagctttgt ttgtaaatgt tatagctaat
     4981 atatttggtg cttgtacacc tttatcttct ataagatatg ctattcttgt tgttaatacc
     5041 ttagttttac ctgaaccagc accagccagt ataagtactg gaccttcagt cttttctact
     5101 gcttctcttt gagcaggatt taatgtatct aaattcatat ttctcctcct gttaacagcc
     5161 ataaatatat tttaatctaa aatatgaatt acttcaaatt ataattatgt attatattaa
     5221 tagattattt ccaatatatt acaattaaat aacatttttc catataattt atttcattgt
     5281 ttaataaaaa aaataacctt atgatttggg gaatcataag gcacctttaa ggggtctagg
     5341 ttaagggtta ttatatataa atctattttt ttaaaatagg tttagggctt aattatttat
     5401 ttgtattaag ttctctttcc ttccaatact tatacaaatc ttcttctttc ttaaataagg
     5461 gtaaattcat atttctatgt acttttacta accatgggga ttctaggcca gcttgtataa
     5521 tattattttc atttataaac acattttctg cttttccttc atcaatcaaa atccccttat
     5581 ccaatacata tatgtaatca catatttcat acattaaatt catatcatga ctggatatta
     5641 ctatttttat attattttta tttaatccct ttattatatc tacaatagac ctagtactta
     5701 ctgggtctaa accagctgta ggttcatcaa gtaatacaat ttcattttcc attgctataa
     5761 ctgatgctat tgccactctt tttttctgtc catagctaag aaaatgtact ggcctgtcta
     5821 taaaatctat tccattaaca gctactagag ctttgtttat tctctcttta attgtttttt
     5881 catccatacc aatgtttctc attgcaaagg ctatatcatc atatactttt gaataaaaaa
     5941 tttgtttttc tgggtcttgg aagacaatac ctacattttt tctaaggttg tataaacttc
     6001 tcttatcata ttttaacttt tcttctttaa aaagaatttc tccagatgta ggctttaata
     6061 tacccattaa attcataaat aatgttgatt ttcctgaacc atttgaacct attataccta
     6121 taacatttcc ctttgaaaaa tccatattaa tgtttaatag tgcattggta tttttttcat
     6181 attgatacgt taaattattt attttaaaca tcattatccc ctactatgtg aaatttacca
     6241 tcatacaatt ttatgtctaa tgatatacac atatcatcat accttgtcat aagtcttttg
     6301 tacagcatac ttccaagaat acctaaggat ttatatgaag tttttaaatt tatatatcca
     6361 aatcttagct cttgtgactt gtatatttct gaaaattctt ctaaaaatat gaatataaac
     6421 ctatatatta acattgataa ctcgacaaaa gtgtcaggta aatgaagttt tttaaaaaag
     6481 aatattaatt gattaaaagg tgttgtaagt atacaaaaat acacacaagt caaacaagac
     6541 atcgctctaa ataacacatg aatagacatg ttaatagacg cctttgatat tcctacatta
     6601 aaactaaaaa attcaaaact atataataaa tctgctttat taaaagatat atttacaaga
     6661 gttattccta tactaagaaa taaaaagtac attggtattc taagtaattt taaataattt
     6721 tttaaatcta ttccagccac acaaacaacc aaaatactca ttaaaatcat aattccactt
     6781 aaaataaata tattttgtat taccatagaa gcaatcagaa aaattactcc tatggctact
     6841 tttttgtttg gatttacttt actcaatcta tttgtatatg catatttatc aattataagc
     6901 atgattaact tctctttcct ttttgaagac ctaatatgta tcctattaca ccagccccta
     6961 aagctgcttg tgcagagaat aataaacttt ctatttctcc acttggtggc tcatatggac
     7021 tgctaaacca tggtttgtaa tttggattta tcttagtaat ttcactttca gcttgaccat
     7081 ctgctcctcc atattcagca cctgagttga caagtaatgg gaatataata agagctacta
     7141 ctaataaaag taataatatg ttagtttttg tttttgtttt agcactcatt agctttaact
     7201 cctttatcat tattttctag tatgcttttt tctccgtttt ctgtaattaa attatataca
     7261 attacagtaa gtataccttc tgctattgct attggtattt gagtcataaa gaaaattcct
     7321 aaaaatttaa ttgctgaagc cattacccca cctgatgggt ctgggaaagc cagtgctaat
     7381 tgtattgaag ttattgtata tgtggctaag tctcctattg ctgctgcaag aaatactgga
     7441 atagcattat ttccatcttt tttctttaaa gccttaaata ttaggaatga tacgattggc
     7501 cctattatcg ccattgaaaa agcattagct cctaaagtag ttattcctcc atgtgctaat
     7561 aacaatgctt ggaatattaa aactatagtt ccaagaacaa acattacgct tggtccaaac
     7621 attattgctc caagacctac accagtagga tgtgaacaac ttcctgttac tgaaggtatt
     7681 tttagtgctg ataatacaaa cacaaatgct ccacataaag ctaataatac tttcttttta
     7741 gggtcttgct ttactatttt acctattgat tttaatccga ataagaaaaa tggtataaat
     7801 ataactcccc atgctataga ccattttact ggtaagtaac cttccattat atgcattgca
     7861 tttgatgtaa ctggagttaa aatgattaac ataagtgcag ctataacccc taatttaata
     7921 ttctgtttca tcttagtatc cctctctccc tttctaataa ttaaaacata ataatacatg
     7981 tctgatttat ttaataaaat aaaaaagcct aactcgatgg ttaggcttct aattttccac
     8041 aatctaaata caaacacata aaacaaacat atctgtataa ttattataag aaactttctc
     8101 catccccgaa gaaattactt taatatttta ggcaggtctc ctgacttagc ttcatcctac
     8161 tccttttcct tcccatgaga tataaaaaat atcatcatag tggctaacaa aagtttcgtc
     8221 tgcatcacag tagcgggggc tgtaaaggaa tttaaccttt ttccctatta atcttgctta
     8281 aagaacctat aatattatat atttaatttt taaattattc ttaaatcatt ttctatataa
     8341 atcattgtat accttttctg tgaaaatttc tacatttttt ataaatttta ttcattttta
     8401 tatttttatg tatataaatt cttttttgta agtttattaa tttctaatta tcttctttaa
     8461 atacattgaa actacatcag aaaatctagt tatatctctt gaatatataa aatcatttcc
     8521 atatattaat ctttcagctt caagttctga ttcttttcct gtaaatactc ctaatactaa
     8581 tatattttgc tttctagctt ttctcacttc attggctgta tctctcaaac caactatacc
     8641 cctataagcc ttttctcctc ttaatgttct ctcactactt tttcctatct ttacatcatt
     8701 tggtcttcca tcgctgagaa ctatcaggat tttattttct tcatctctat ttattagacc
     8761 actacatata gattttattg ccaaaccatc tctattatta ccagcacaaa aatattcaaa
     8821 tatattttca ctatttttta tactatcatc ataatcttta aatcttttta atattgtata
     8881 gtctaaaaaa ctagaaaatc ccatcactct atttggtatg ccaacttctg ttagagcatt
     8941 tgcaattatg taagcttgaa ttgccacatt tccctgattt ctactttgag agccacttgc
     9001 atccaataat atatcaataa cgtatttccc tttttcattt tctatatctt tataaaatat
     9061 tttattatta ttacttctac caactctcca agctctattg gctaatatag taccacaatc
     9121 agaatatact ctacttttag cattttcttc tattaaaatt cttgacagac tttcttttaa
     9181 tctctttatt tgctgtctat acattctagc atgttctcta aattttccta aattattttc
     9241 tttttgtctt gttacatatt ttagttgaaa tacattatta cactcacttc taagaactcc
     9301 atctgtaaag tgtattctac aaccttcatg aacatttcta caattttgtg tctcaatttt
     9361 tttaatttca cctttactca aataagtttt tccataataa tgttctattt ttgaatatat
     9421 cttatttgca gtgtcttcat caacatatat aactcggtct gctgaattgc tacatttaga
     9481 atcatcaaaa tcccctacac cttctacaag catggatgaa gatattgaat ctatatcact
     9541 ttcaataata ttttcttctt catcactata aagttcttca tacatgaaat cagcaaatgt
     9601 atcaaaattt acttctacac tttctacatt attttcatca ataagactat tttcttctcc
     9661 atttaatatg tattcaaaat aagttaaata tataacatct aatgctttta aaatctccat
     9721 aatatcttga ttggtatcta tatttcttat atcattaact attcttctag tcatactatc
     9781 taatgctggg tgcttgtcca tactataaag tacaaatgtg tatttaattt tatccataat
     9841 attatcattg tgtattttgg taaaacgtga aacaatatct ttataagcat tccttcttat
     9901 gtcttctatc cctggcctgt ctttaatgac cttttcttca actattgtat ttaatataac
     9961 ttgaattaga cctagtataa catctttgtt gtatcctttt cttattctat ttgtaagata
    10021 ttttttaact atatcccaat ctacatactt tcttctagca cctgcaatta ttgcgaaata
    10081 tagacctatt tcttttgaag agtaatcttt ttctgatata tctatacttt cattgtaatc
    10141 accagctatt gtccatgata ggttattagc tctattttca aattcataat catcatatat
    10201 ccatttcata atctcaccta tattaaatat gacttattgg aaacacatct atactctccc
    10261 atttattagg tattctagtt ttaattacat ctccaaccat ttctttctca tatacatcaa
    10321 aagtctttcc tgtaagtccc atattaattg caagagttgg ttttaatcct cttcttatag
    10381 tcttaagaga tgccataaga ccacgtaaat caatagcttt actagatatc tctccatttt
    10441 gagacttcaa ttgtaaatct aaaaatattc ctgcaaagtg tattaatttt tcttcatcag
    10501 catctgaaaa ttcatttttt aaaatcatca ttaacttatc ttcttctata ggaggaatat
    10561 ctattaccat aaatctagat accagtgctt cattaagttc ttttgttcct gcatattcat
    10621 aattcatagt cccaataaat cttgttgctg gatgtagatt gactctctca tacccaggta
    10681 catcaattat tcttctatag tctaaagctg aatgtaccac aactatagca tcattttttg
    10741 ccatattaat ttcatcaaac accccaaaac ctccattaat agcacactca taaactgagc
    10801 ctcttctaag tttaacttca ttgtctataa atgtatctgt acctataagt gttgaactgt
    10861 ctgtatttat atgaaatgat gtattccact gaggtctatt aaatatttcc cctagattat
    10921 cagcaagtaa gttttttcct gttgcctttg gtcccgataa taatatattt tcttcttcta
    10981 aaatagctgc tatacacata gacaagatat ctttaccata aaaataagtt ttagactttg
    11041 ttactctgta ttctaaatct ttttctaaac cataataatt tctaaaatac agtacatctt
    11101 caataagttt ttcatctacc ccttgcaccc ttagattatc tattatatcc atatttttac
    11161 cccttttata taaatagtat ttgtttcatt tcttatcacc attttagcat aaaaggatat
    11221 aagctaaaat tgatttttta atctccccat tttaagtaaa accatgcacc attttatcac
    11281 tacaaaatta agagtactac ttactgatat ttcttgattt tgttcgtcta tacgtatata
    11341 atatatgctg ataaattatg aggaaaggat gattgaaata tggatgccca gaagtagcaa
    11401 aaaaatgggg tatttctgaa agaagagtac aaaaactatg tgaagaaagt cgcataccag
    11461 gagtttcaaa actcgggtat atgtggctga taccaaagaa cgcagaaaag cctgttgatg
    11521 gtagaacaaa aagaagaaag gaagtacaat atgaatagcg ttttgattat agatgatgac
    11581 aaagaacttt gtgctcttat gaaaaaatgt gttgagcaag aaaatttatc tgctattact
    11641 gcacattgtg gtgcagaagg tctacatttg gtcgataaaa atagacacac actctctctt
    11701 gtaattttgg acataatgat gccagatata gacggttttc aagttctgaa aaaaattaga
    11761 gaaacaagta atattccagt aattatgctt actgctaaaa atgacgagga tgataaagtt
    11821 tccggactac gtttaggtgc tgacgattac ctaacaaaac cgttcagcat aaacgaactt
    11881 atggctcgtg tcaattctct tattcgtcgg tatactatgt taaaccctgc ttttataaca
    11941 gacacagatt gcataataat aaatggcatg attattgaca agacaaaccg aattgtttct
    12001 gtcaataata ttcctgttca acttacaagc aaagaatttg atttacttgc ttttctggct
    12061 tctaataagg gacgggtgtt taccaaaaaa cagatttata cacaagtatg ggaagaagaa
    12121 tatgcttttg acgatagtaa catcatgtct ttcatcagta aattgagaaa aaagattgaa
    12181 ccagacccca accatccatt ttatattttg acagttcgtg gtgtcggtta tcgttttaat
    12241 aaggaggctt aacatgaata caagtgtcta tctgcttttg atattggttc ttagcctgat
    12301 tattatagcg taccttgttt ctgtcctgct acgtgtccga atacaactta cacttataaa
    12361 agatgcttta gaggatataa aaaatggaaa ccttaatcga cgtattttaa cagatgaaaa
    12421 cgacattaca aaacaaattt gctatgatat taacgaaata gcaatcaata gccaatttca
    12481 acttattcag caaaaacaat cagaacaagc ctacaaacag ctaatgacaa gtctctccca
    12541 tgatgtaaaa actccacttg cttctttggt cggttatttg gaagcagtag aaagtaaaat
    12601 tgttgtagga gaggaaaaag acgagtatat ccatgttgcg tctaataagg ctcattactt
    12661 aaagaatttt gtagaaaatc tatttgaatg ggtaaaattg gattctaagg aacaagtttt
    12721 tcattttgat atttttgact tgaatgaatt atcccgtaat attatatctg attggatacc
    12781 tgttttagaa agcagtcatt ttgaatatga atttgatata ccagaaatag agtacttctt
    12841 acgtatagac gcaaatgcat atacccgtat cattaataac ctattacaaa atattattac
    12901 ccatagtagt ggcaacaaaa tgacattgag aatttttgaa aacaaagagc aagctcaaat
    12961 tataataaca gataatggga aaggtatatc ttcagacaat ctcccacata tttttgaaag
    13021 gttgtaccaa tgcgaccatt cacgagcttc aaagggaaat ggtttaggct tggcgattgc
    13081 aaaggaactt attaacgctc ataagggaaa tattaccgcc aacagcactc ccggtatggg
    13141 aacagaattt acaatattgc ttcctaaagc cctgtaaaag tacgaggctt tcttactcta
    13201 aaacaagata aaagcaagat tatggcaagg ttttggcaag gttcatgttt tataattcta
    13261 attgtagttg attttgacgt tgtatcaact atcttttgta gaaacaaatt tattaaaagt
    13321 gaggaaatta tatatgaatg attttgtgat tgaaacaaag caacttacaa aaatttatgg
    13381 gaatcaaaca gtagttgaca aagtcaatct ccatgcaaaa aaaggccgaa tatatggtct
    13441 gttaggacgc aacggagctg ggaaaaccac aattatgaaa atgattttag gattgacttc
    13501 aatcacgtat ggcgaggtag atgtatttgg agagaatatt aagggacgtg aaaaacaagt
    13561 atatccccgt atcggtgcaa ttatcgaaac gccaggattt tatcctaatc tgacaggaac
    13621 ggagaacttg gagatttttg caaaactgcg tggtacagcc gcccctaatt ctgtaaaaag
    13681 agcattggaa attgtaggct taccttataa aaataaaaag ctgtttagtg aatattcact
    13741 tggcatgaaa cagcgacttg gtattgccaa cgctatcctg catgacccag aactgttagt
    13801 tttagatgag ccaaccaatg gtcttgaccc tattggtatt gcggagatga gaaagtttat
    13861 tagggacttg agcgttgagc atggaaagac aattcttatt tccagtcata ttctttcaga
    13921 aatctcattg ttggctgatg atattggaat tatcgaccac ggtgttcttt tggaagaaag
    13981 tagtatgagt gaactagaaa agaaaaacag taaatatatc ctgctacaag tttcagatat
    14041 tccgaagaca tcacttattt tagagcgtca atttaacacg aaggattatt ccgttcaaga
    14101 tgactacacg ttacaccttt ataacactaa tctggatatg ggtgaaatca ataaagcact
    14161 tgtaatgcag aatgtaactg ttattagttc tcaactttgt aatgatactt tagaggacta
    14221 ctttaaaaaa atcacaggag gagagggcat tgcttaaact tattcaaaca gaatttctta
    14281 aactgcgtcg taggaaactt atttggctga tgttgttgtc tgctctcatt atgccgtttt
    14341 tcgcactact atacttcaat tattttggta aaacaggtgt tgagccaata aagttttata
    14401 aatggtctgc atttggttat acactttgga ttattttgcc tgttgttttg ggtatatttt
    14461 gcaccatgct tatacacgaa gaaaaccagt atgatatgct caaacagctt tggattgtac
    14521 ctattagtaa aatgggatac ttttttagta aatttttcgt tgttttgatt tactctattt
    14581 gttttatgct gattacagcc gttgcttctg tgttatttag tgtactttct ggatatgtcg
    14641 tatttagctg ggcaagcatt ttatatttgt tgaaaaaatg tttagaaatt ggtgtaatca
    14701 ctgcttttgt aatgttgccg attttggcaa tcgctacatc tcaaaaagga tacattctcc
    14761 caatttgtat cacattggta tatgcctttt ccggttttgt tataatgaca ataaatatgt
    14821 atttacaccc attatccagt atgggagtta ttatcatgag aaataaagac attccaggtc
    14881 ttatattttc acaggaaatt aatgtcccat tagcatttct ttgtatttgt atttggagta
    14941 ttgtatcagt attgtttgca aacactgcac taggaagaag aaagtgaggt gtagataatg
    15001 tggaaattac tttgggctga atgtcaaaaa attcgtcgtt caaaaattgt ttggattaca
    15061 gtttttgcat ccattatgat agcagtcata gtttttgcag gagggcagga tgtatataat
    15121 gacccagatt tacattatgg cttaaagagc gtttatgacg gttcacggta tatagacaat
    15181 gcaggttggt atatggacgc agtacaacct tggtcaactt tttttgttct tcctgctgtc
    15241 attgccctat taggaagcta tattatttgt cgtgaagaag aggaggacac tattaaatcc
    15301 ctgcgtctta ttcccataaa tgaaacaaaa ttaaccatag caaaaatgat tattactttt
    15361 atattcagta tacttcttta tttgttactc tttactataa catttttgac tgaatccgtt
    15421 ttacattttt ccgatttatc gacaaaactg gttttaagtt gcatgaaaga gtattttctt
    15481 gatggcatag gtgtattctt tgcaatttct cccattattg ccttggtatc gcgcatgaaa
    15541 aaaggatatt ggcttgcatt agtattcaca gaagtttatt ctattgctgg actatttgcg
    15601 ggtatgtcaa atgtcttaca aacctttttt cctattgcgg caatatttaa tatttctgga
    15661 tactatataa ctactggaga aaaaattatg gggagtatca taagtttatt actttgtgcc
    15721 tgcctttctg catttatact taaagggcta aaacacaatg aaaaaaattg aaataggtat
    15781 atcaaaatga aaaagataaa aaaactgatt attctaattg tattcttgtt tctttttggc
    15841 atgttacact gttatactga atggaaattc aacaataacc cacctaaagg ggtaatagaa
    15901 agaataaatg aatggactga taagattgga ctggattagt aacttatgtt aattatagtg
    15961 aatgtaatat tagcaagtta ttgtttcgta ttttaatact ataactttaa atatttggtt
    16021 ttaatcttcc aaacgaatat aaaaaaactg tcacagaggc aacatatttt tgaatattta
    16081 taaagtggta gttttgattt tcaaagctgc cgccttttct tctatataag caaacttatc
    16141 ctcaacaaag tgtaaaacat ggtacatttt aagaaaaaag taccacagac acaagcaata
    16201 aaagaaacgc cgagaaccta caaaaacgta gtgcttatgc agatttacga cgccttctat
    16261 tttctcaacc gtcttaatga ccttgtacaa aagaaatttt tctaatattt tttataatct
    16321 tatcttgtga tgttttttat gtataaatga cttaataaca aatccaataa aaattatgga
    16381 tattggtata attagaacga ggaatttata atcattataa tgtgacatta ttcttaccca
    16441 actctcagaa aaaatatatc ctatactgat taatgaagta ttccatatgg ttattcccac
    16501 gatagatgat attacaaaag ttataatatt ttgacctgct atacctgcta taaaagatat
    16561 gtatgttcta cataaaggga ttaatctaca tacacaaaca gaaatagaag agtatttgtt
    16621 aaaataatct tgagatgcaa atattccttt ctttgccttt ggacatatct ctattatttt
    16681 atttatgata gatttacctc cccaatttcc cactgtataa caaaatatcg aaccaagtat
    16741 acctgcaatt atacttaaaa ttaaaattgt cagaaatcca aaatgatttc ttgcagcaat
    16801 tgctccacat aaaggtaaaa caacttcgct aggtaatgga aaacaagcat actcaatcat
    16861 aattaataca aatacagaga ttaatccata ttgtaaaata aaactattga ttacagacac
    16921 ttacttatct atctcctttc ttatttaaag gttttaaggg tacatgttct aaggattctc
    16981 accctatatt taaagaatca atctctttaa actattatat gtatttacac aaagcattta
    17041 taacatactt taaaaatagt accttaaaat aagaaactta ataaataaaa aaa
//
LOCUS       DAMJZE010000083        16941 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJZE010000083 DAMJZE010000000
VERSION     DAMJZE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.084"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(163..2301)
                     /gene="vanT"
                     /locus_tag="PC541_002245"
     CDS             complement(163..2301)
                     /gene="vanT"
                     /locus_tag="PC541_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2839708.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2319..3125)
                     /locus_tag="PC541_002246"
     CDS             complement(2319..3125)
                     /locus_tag="PC541_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2839709.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3122..4222)
                     /gene="vanG"
                     /locus_tag="PC541_002247"
     CDS             complement(3122..4222)
                     /gene="vanG"
                     /locus_tag="PC541_002247"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2839710.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4483..5565)
                     /locus_tag="PC541_002248"
     CDS             complement(4483..5565)
                     /locus_tag="PC541_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2839711.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(5615..6316)
                     /locus_tag="PC541_002249"
     CDS             complement(5615..6316)
                     /locus_tag="PC541_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2839712.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6750..9281)
                     /locus_tag="PC541_002250"
     CDS             complement(6750..9281)
                     /locus_tag="PC541_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2839713.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9389..10006)
                     /locus_tag="PC541_002251"
     CDS             complement(9389..10006)
                     /locus_tag="PC541_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2839714.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(10368..11252)
                     /locus_tag="PC541_002252"
     CDS             complement(10368..11252)
                     /locus_tag="PC541_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2839715.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(11443..12693)
                     /locus_tag="PC541_002253"
     CDS             complement(11443..12693)
                     /locus_tag="PC541_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2839716.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(12915..13679)
                     /locus_tag="PC541_002254"
     CDS             complement(12915..13679)
                     /locus_tag="PC541_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839717.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(13673..14398)
                     /locus_tag="PC541_002255"
     CDS             complement(13673..14398)
                     /locus_tag="PC541_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839718.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(14400..14768)
                     /locus_tag="PC541_002256"
     CDS             complement(14400..14768)
                     /locus_tag="PC541_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2839719.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(14944..16488)
                     /locus_tag="PC541_002257"
     CDS             complement(14944..16488)
                     /locus_tag="PC541_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2839720.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         5825 a         2979 c         1685 g         6452 t
ORIGIN      
        1 aaaaaggtga tgtttcaaaa taaaaatctg aaacatcacc tttttataac ttaaattgtc
       61 atgtaagtta taaaaaatat atattagaca taatattact aaaaatgtct agtcagagta
      121 aaataaatta cacagtacaa caaatattac tatttaattg agttacttct ttataacatt
      181 taaccttctt cctactctgc tcaataattc attcgatata ctacatgagt tatttgcaac
      241 ttcagaagca gataattcag ataaattatc tcttcctatt attattgccg tattaccaac
      301 ttctacatta ggtatatctg taatatcaac agcaagttgg tccatgcaaa ttcgacccac
      361 tattgctgct ctacaaccat ttattattac gtaacttttt ccacaggata aatttctagg
      421 tataccatcg gcatagccaa tagataatat agcaattacg ctatctctgc ttgcaataaa
      481 gactcttcca tatccaaaac tatcatcttt ttgtattttt ctaataagaa ttacttgtga
      541 ttttaaggat aaaacaggtc gtaaatcaag ttgcaattta gtcgtatcat ttggagaact
      601 taatatcccg tataatgcaa ttccaattct tacataatta cacttaagat tgggataatt
      661 aaaaaaacca taactacttt gaatatgtac tttaggaatt tttatacctt tttttaacag
      721 aaaatcaatt aacttataaa aacattcaat ttgtttattt gtaaagtcta catcttcatc
      781 tttcagacta tctgaaacac acaagtgtgt aaatatgcca catatatcta agtattttaa
      841 tttaaatgcc tttaaaattg acctcacatc attaacatca aaacccagtc tgtgcatacc
      901 tgtatcaatt ttaatgtgtg attttatgtt gtatttctgt ttgtttaaag agatagcatg
      961 attaaggtca ataacactct gtgtaagatt gtatttatga agttcctttg cccttattgg
     1021 gttagtatat ccaagaatca atatttctcc tagtattcca tatcgacgta aatcaatccc
     1081 ctcatctatt gttgctactg caaatgcttt tacacctatt tggtttgcac aagtcgctac
     1141 ttcaaaagca ctatgaccat aggcattagc ttttacaact gccatcaatt cacaatcttc
     1201 tggcatagct ttttgtatca tctttacatt atgttccaaa ttatttaaat caatttcaat
     1261 ccaagctcta ttggtattta ccttatgttc tcctttcaag tcttttttta gaggtttaag
     1321 aatcaatata ataattatag aagctataaa agatgctata cttaccacta tataatgaat
     1381 aaggctattg tcaaccaaga cagtttgtat tccgaatatt ttagaaaaca tacgaataac
     1441 aacaatcatc ataggatgta taatataaat aattaatgat gatgttcgca ataacctagc
     1501 tcgagaacct ttcaaaaatg ttaaggctgt aaataagaaa tacatacatg gcaataacat
     1561 aatatacatg ctatcatgac gctgtacccc tagtttatgt aaaaacattc cctcacataa
     1621 cataagtaag aatgatataa gaaatccaat caagctattt ttagttgaca tataattaga
     1681 tttattagat attattccac caagtatgaa aaatacaggt gcaaaaaata taccatttcg
     1741 tgtataatct gatacttcaa aaatataccc atataaattc tttaatagtg gtattttttc
     1801 taaaaaacca taataactat caccaaacat accaattaca taaataacaa tagtgattac
     1861 tagtgagtac tttaaaccta gtctttttac aagaaagtat gcaatacttg caccaataat
     1921 tgatgcaggt agataccata aatgatataa agtcccatca aatacaatgt ccttaataat
     1981 attgggcact aaattattca tggaaaaata tccagtgtaa atatttaagg gaatataaat
     2041 tacaatagct atcccgtaaa taaataatac attttttaga aaagatttca gtttattaca
     2101 attataatta tatgttgaaa ttagaaaaaa gcctgatagc ataaaaaaga atggcacagc
     2161 aatacgagca attatactag ttaacacaaa gtcagccatt ttactatatg ataataatgg
     2221 agatgtatga attgcaatta taagtaatgc agctataatc ctaaaataat caagccctgc
     2281 ataagattta ttcctaatca ttcttgttcc tccatttctt agtcctttct atttctccaa
     2341 aatgtcacaa taaaaccatc cttattgtta cccgatattt gatatacaca ttgttcaggt
     2401 atagagattc tcacttcttc tttagtatga ataggcacat aaaagatttc tataattttt
     2461 tctttatctt ctatttttaa acaatttgta tacgaaaatc tcttaatata ttctatatat
     2521 tcttcaagag cgagattcat tttattgata atctcagaat gcggatatcc cacataacga
     2581 aaatgccatg gttcttgaga tattcctgtt atttcttctt ttttacactg ataacgctct
     2641 ataaaaccat aatgtagtgc tgccttacga aactcattac aaataccatt ataaggaaat
     2701 tcagggcaga taaaatctat aacttctttc tttaacccta aatcaatagc aagccctgtc
     2761 tgatgttcac tgtgatttgg aagtgctaca aactttttag taaagtcttt tccattgtca
     2821 cgcagagaac ttgaatatat atcttgttgt tcctctgcag tacgatatcc acttacaggt
     2881 actatttcat ttattccaca tatacttttt aaaatatttt gtaaaatgtt tgtagcttca
     2941 cgtttcataa gaatacttgg aaaccttgta tctacaggaa ttaaatctat atcttcagat
     3001 gttttaactg gtaaaaaagc atttactaga attagattcc catcataaac tttatcttta
     3061 gtcaataaga tagtatttgt cattttatct tctttatttt tatctaataa aatattattc
     3121 atcattttat gtacacctca attatcttgt ccaatatttc ttcaaaagac aatccaatac
     3181 ctttcatcat atttggataa cgactatgag aagtaaagcc aggaattgta ttgacttcat
     3241 taaaaataat ttcaccagat ggagttaaaa acatatctac tctagcaaat ccagaacatc
     3301 caagagttct ataaataatt tttgctgtct cttgtattct tttctcacaa tccaaatcaa
     3361 ttctagctgg catatgaatt tttgaacttt tcaatgtata tttttctgta taatcaaaaa
     3421 atccttttga caattctatt tcatctactc ttccaacaag taagtcatcg tttcctaaaa
     3481 ctgcacaccc tacctcaaaa ccatcaatat tttcctcaat tattacttca ttatcatgtt
     3541 caaaagccaa gtctactgct acttgtaact cttctttttt ataaatttta ctaataccaa
     3601 atgatgaacc tgcacgcaca ggttttataa ataatggata agttaagtct tttgacagtt
     3661 cagccacatt aaatttatct gagtatttta gtaatattga ttttggtact ttaatacctt
     3721 ctgaatgaac aagtttatgt gatttgtctt tgtccataca taatgctgaa gaaagagtac
     3781 cacatcctat aacctgtata cctgccaatt caaataaccc ctgcactgtt ccatcttctc
     3841 cattttttcc atgcagtaca ggaaacacaa aatcaagttt agtgtattta atatcatttt
     3901 cacatgattc aatagcacta tttggaatat gattctgaga aacagcatca ggaattaaaa
     3961 attcaataat accatttttg acacggttct gcgaaacaat aactggaatt atatcagtat
     4021 ccctttcaaa ccaagtgtta ttctggatat tattataatc tccaatataa tgataccaat
     4081 taccctccct tgtaattcca attggtataa tatcatattt ttccgtattc aaattctcaa
     4141 gtactgcaaa agctgattgt aatgatacat catattctgt tgagtttcca ccaaatataa
     4201 ctgcaatttt tttattattc atctttgatt ctcctttgtt gatttattta tttttataca
     4261 atctacagaa acatgttaaa atactttttc tatttcaaat ccaatcaacc ttcacttaca
     4321 attgcatctg ttccacataa tatattttca catttttcta ttgatataat tactgtatca
     4381 aaataaaagc actcttgttt tgatacagta attatatcaa taacaagagt gctctttatt
     4441 tatttatcct aaagaatttc ctattttttt atacttaaat tcttacaaat ttcctacaat
     4501 cggaataata accttaaaga taattatatt gttctcgcta tgagctgtta ttgtcccgtt
     4561 atgaagctcc actatttctt tagctatagc aagtcccaac ccagctctac cactgtttga
     4621 attacgagat gtatccaacc tgtaaaattg ttcaaaaata cgctcaagtt tttcctctgg
     4681 aatcgtattg ctatgattta taaatttaag ctttatataa tcatcctcct gtacagctgt
     4741 tatttcaata gtagtatctt caaaactata ataaacagca tttcgtaata agttatcaaa
     4801 tacacgttgc attttattaa catcacacct tatcatcata tttggtgtaa cattcagtgt
     4861 atattttaaa ttcttactca tcaaagtagg tttaaactca tatgttagtt gttcaagcat
     4921 acgagttaag cttactgtac tgtattcaag tgatatattt gataaattaa accttgtaat
     4981 ttcaaagaat tcattaatta aatcttcaag acgctctgcc ttatccaacg aaatagataa
     5041 atattttttt cgcagttctt cagaaatctg actttcatct cgaagtagtg tcagataccc
     5101 tataactgat gtaagtggtg tttttaaatc atgtgcaaga taaacaataa gttcattctt
     5161 acgttgttct gccatttgtg tctcaaattt acgttgttca agtatatgtt taattgagtt
     5221 tattttcttt tctattgcca ataactctgg agataaagcc acttctccaa catcttcttt
     5281 gataagagag tcaatacctt gatttatttc actaaaatat tttgtaaacc aatttaaata
     5341 aacccggaat attataaaaa acacaatcac catagctagt atcatgaaga gctccatgct
     5401 attacggaaa atctgctgat ataaattcaa tgcagcttta tattccatac gaaaagcatt
     5461 ttcaaagaat gatactatcc agatagaaaa cttaccatac aatataaaag aatataaaat
     5521 ccatatgaaa ataatggcta taaatgccat taaagctgtt ctcataaaaa ttttatttct
     5581 cacttgagta aagtcaggat tgactttaat atttttactt ttcaattgta taacctactc
     5641 cccatacagt ttttataaat ttcgggtttt tagaaggttc atgaattttt tcacgtaatc
     5701 gccctatatg agccataact gtgttattat tatcaagata tttttctccc catacagctt
     5761 caaacaattc ttcagaagga accacttttc cttgatgttc acataaatac caaagaatag
     5821 aaaattcaat cggtgttaat gctacttctt tcccaaataa actacattta tgtgtttctt
     5881 tattgataat caatccttta atatcatact cattaacaat tatcgactgt tgttcataag
     5941 aattattata tcgcatatat cttcttaatt gtgtctttac tcttgcaaca acttccaaag
     6001 ggttaaatgg ctttgttata taatcatcag cacctatggt taaacccatt atcttgtcta
     6061 tatcttcaat tttagctgtt aacataatga taggataata gtatttttct cgaattttct
     6121 ggcaaatatg aaatccatca atatcaggaa gcataatatc taaaatagcc atatccaaat
     6181 ttttagattc aatacattct agagcttcct tacctgtata aaacttgtat actgtaaaac
     6241 cttcattttt taaatatact tcaattaaat ctgctatttc tttttcatca tccacaatca
     6301 aaatattttc gttcatagat tatttttcac tctccttatt atcgaggtca tcatttataa
     6361 tattggtaaa tttacatatc aaataattac aagcatattt gtaactaatt tttataagaa
     6421 aagaactact acatcagaat gtgttgatgc tactattgga acacaaaaac tgagattaat
     6481 tgttattata aaaactcccc taacacgtcc ttattatcgc ataacaggta ttaactttca
     6541 ataaaaatat ctatcagtca aacacaattc aatttattat accagagaca atccatataa
     6601 aaaacaatta aacctatgta tgtaaccaca gacattacta aaatttccat gttattttat
     6661 ataaaaattc aaaaaaggag ttgaatttaa aatttgatta gaaaaaaatc aattttgaga
     6721 caactccttt ttattttaat gtatttaaat tatagtatat cagccttcat aatatcctca
     6781 aaatcacctt tttcagatat taatcttatc aatttaacag tacagcttaa atcatttata
     6841 agtatagctc caactaatga accatctttc ataaataatt ttttatatat tctttgacct
     6901 tcatcttttg atgaaatttc tttatcaaaa ttttgtatat taccaatact aaatactttt
     6961 acattcattg catctaatga aactggatat acttcacttt caaatgtcaa attatctcca
     7021 cttgcatttg cccctgctat ttttcccatt tctactgaag atggccatat tgctaaacta
     7081 atatttccca cttgtgcaac atctccacaa gcatatatat ctttaacatt tgtctgtaat
     7141 gtcttatcta caacaatacc tctatcaatt tttacagatg tatcctgaac catttgagtg
     7201 tttgctctta caccaattga aaatactacc atatcacagt cgacactatc tccatcttta
     7261 aatacaactt tttgagcttt tccatcacct tcaataccat caactgctac acccaatctt
     7321 acatctattc ctgtatctct tacacattgt tctaatattt tactaccttc tttatcaagt
     7381 tgttttgata gtatgctatc catggcttca actactacta cttctaatcc ttttagtcta
     7441 aattcccatg cagcttcaag tcccaataaa ccgccaccaa tcactacaac tttcttagat
     7501 ttttctagtg cttcttttac actatataaa tcttttatat ttctaagagt aaacacattt
     7561 tctaaatcat ggcccttaat tggtggtata aagtttctac ttcctgtagc tattacaagc
     7621 ttatcaaatt taatagctcc atcattcact ataatagttt tattcacttc atctaattta
     7681 tctacattcg ttcctaatat tactactata ttatttttat cataccaatc tttatcttcc
     7741 atgtagaaat ctgaaccaag ttcttcattt ataccatcag aaagtgcagg tctatagtaa
     7801 gtcaactcat cttcattaga aatcatagtt atcttacaag ttttatttct ttttcttata
     7861 gcatctgctg cataaaaacc agctgctcca tttcctacta ttacgaaata ttcatcagta
     7921 tcttttctaa aagtaacttc atcttcttca acttctataa attggtcttc cttagctcca
     7981 catacagggc atacactagg agcactatca ccttcaaaaa cttcattaca aattacacat
     8041 ttaaatttct tagttttctt tttacttcca aaagtagaat ttaacttatc taggaattta
     8101 cctgtataag aatataattt atcaatctct tcatttgatg gtttaaagtt aacagcaaaa
     8161 ggttttactg ctgtcattct aagctgactt attctctcca tagcattttc tataccttct
     8221 ccactccatc catatgaacc aaatactgaa gcaacttttc caccatgaag aactgggttt
     8281 aacgatatta atatatccca tataggcttt aacgcatctc ctaatattgt tggtgttcca
     8341 aataatacac cttgtgatac tgctattttt gccaaaatat cttcttgctt atgctctatt
     8401 acatcaaata aatttacttt atagttgcta tttttttcaa tatatgcttt tatagcttct
     8461 gccataatct ttgtatatcc atgtgcagaa acataacaaa ttgtaacttc tttttctaac
     8521 tttatttgtt catttacact ccagttgtaa taaaggtcta taatttttct aggattttca
     8581 gttaatactg gaccatgacc tggacataca gtatctatat ctaaatcttt tatcttttct
     8641 atagcagtta ccatagatgg tttaaatgga cccattatac aatcataata gtatcttaaa
     8701 gcatccaagt aatccttttc ttcattttca tcaagtgtat ttactatctt atcattacta
     8761 tagtgactac caaatgaatc acatgttaca agagttttat cctcttttat atatgtgtat
     8821 atggtatctg gccaatgaag cataggtaca gagaaaaatt ctaaagtttt gtccccaatt
     8881 gataatgtat ctccatctgt aactgcaaca tattcaaagt ctttgtttac gatttcctta
     8941 agatatttaa ttgctgtttc tgaagctact acttttgcat ttttagctaa atccaaaagc
     9001 ttttcaacgc ttcctgcatg gtctggttca gtatgactta caacaatata gtctattttt
     9061 tcaaaatcta tatttaaatc atttaatctt tcaatataat tgtcaaaatg cttatcttta
     9121 actgtctcaa ataaaactgt tttttctgtt ccttttaata catatgaatt ataagtagtt
     9181 ccataaggtg tatacataat tatgtcaaat actcttaaat cagggtcaag cgaacctacc
     9241 caatataaat tttcttttat ttttagtgat ttcaatgaca taccgtactc ctcctaaaat
     9301 agttatttag tctctttata ccctgcttgt tatattttaa acaaaattta ttcaaaaaag
     9361 aaattatttt aaagatagtt taaatcttct aatcttcttt ttctaccttc aatacagttc
     9421 cctttttaga atctactttt atgtcatatt catttttatc tttatctatt cttattttat
     9481 aaaaagtaac atctaaatct ttgtctaaac tccattcttt aactattcca ttttgttctt
     9541 ttaatgcaat ttccatagct tgttgaggtg tcattatgtc atttaaatct aatttttctt
     9601 ttctagcaac tccatttaaa tcttctgaat ccaacttttc tgttttatct tctaatacat
     9661 cttttgtatt agcatcaatt ttcatcttat attcattatt atcatctaca ccttccacag
     9721 tataaacaaa tttatttaaa tctctctcaa gacttaaatc tactatatca gcatccttat
     9781 atttatcttt aaatatgttt attgcatctg tgtatggcac tttaaaatct tgttccatta
     9841 aattttcatt atttttttca tcttttaaag cgttatttga tgcatttgcc tccttttttg
     9901 tattatcttg tgcttgtcct gaatccttag ttggactccc actacatgca gttaataaac
     9961 ccgctataag catacttacc actaataaat acgttttttt acacataaaa accctcctta
    10021 aatatattaa tttattctat attatgccct ctaaaccctt tatttaccct tttatattaa
    10081 aaattcctcc tattttatca attttaaaac tttatattta ctaaaattaa agttacataa
    10141 aaattatact gtctcaaaat ggaaacaagc ctctttttca ttgttgaaat aataaaaata
    10201 tattttattt cgtacaaaaa gagtgcctca tgaactaaaa agttcatttt gagacactcc
    10261 cattttttta tatttttact tacaaaataa cagtacttat taatttttta caaaccttat
    10321 aagtatatct atatgatatt cttaaattta aacactatat taaaaaatta aattttgttt
    10381 aattctttta ccgttaaatc cattttagga tatggaatcg taattccttc ttcgtcaaac
    10441 ctaactttaa cctcttctag taaatcaaga taaattgtcc aataatcttc tgtcttgcac
    10501 caaactctag ttgcaaattt aatagcatta tctccatgtt ctactactcc tataaatggt
    10561 tctggttcat ttattattaa tttatgatta ttaacaatat ctttaagtac tcttctaaca
    10621 tgtagtatat catcttcata ccctacacta aaagtcaaat caactcgtct agtattcttt
    10681 gctgaatagt ttattagact atcatttgat acagaaccat taggtataag tatctgttta
    10741 ttatctggag taaccaattg tgtataaaaa agacctattt gttcaaccgt tcctccatga
    10801 cctgctgctt ctacgtaatc tcctacttta aaaggtctaa tcaatagtat aataaatcct
    10861 ccagcaaaat ttgatagact accttgtaaa gccaaaccta ttgctacccc tgctgatgct
    10921 atcacagcag caaaactgga caaactcatt ccccaatatt ccagaacaat gattataacg
    10981 gcaactttaa gtacactcaa aagaagagac tttaaaaact ttcttaaagt taaatcaata
    11041 tctcttttac taagtaataa cataacatgg tttacaaatt tttttattat tttaaatcca
    11101 atagaaagta taagtatgcc tattataagt tttactccgc ttgtagtagc ccattctagc
    11161 agactttcca ttatacctcc catatccatc ttattaatat ctaaattatt taaatctaac
    11221 ttgcttatat catctatttt cgtcgtatcc ataaaaaact ccttacctta tattttagaa
    11281 ccaatattat agtttaaagt tcttattttc tatttaatat aatacaatac catttgtaca
    11341 tagtaaacaa gatttatatt ccaattatac atattaggaa aatgcaaact atttgttata
    11401 tatatgcaaa atataatact aaattaaact attaataaaa cactatatag ccttaaaatc
    11461 atcatttttt acccctatat gagatgatac tatagttata atacttagta ctataaatac
    11521 tacaaaacct tttttatatc catcaactat attatatgaa tttacttgta aagagatagc
    11581 tataatcaaa ttaataattc cacttcctat aaattcaaaa gtattaataa cactagttgc
    11641 cagtccagaa taaatttctt tatttttata cttggcatca ttaaaaacct gtagatgtga
    11701 cataactata catcccataa taaagaatga tattggtaaa actataattg gtggttttac
    11761 ctggtataca actacaatta caatccatat aaacaaatct atcattgctc caaattttat
    11821 tatattaaat ttgctagacc ttattttagc aaatacaaaa tccataatta ttgaaccaaa
    11881 tataaatcca tatgtaaaaa atgatactat aaacgcagaa aaactcttac tcacaccata
    11941 aacgtccatt atgtatctta ctccccacaa gcttataaat gcacttgtaa gaccaacaaa
    12001 agagaacatt atcattgaat tataccatgt tgatttgttc tttataacag acttaatacc
    12061 atcaacaata ttaaccttct ctgacttttc atagggttca gtatctacat tgaatccata
    12121 ctcttttggt gtatctctaa caataatata cataaaacat ccaattacaa caccaattat
    12181 accgattaat aaaaatgaat ttctccaacc aacaagttca gacaaaaaca caagtggaaa
    12241 agtagcaaga actccaccta aatttcctac aaatgaaaat ttagctgttg cagaagcaaa
    12301 ttctgactta ttaaaccatc tcccttgaat ttttaaaata caaagtaata ttactgatgt
    12361 tcctgcccct acaattacac gagaaatata tgctaattct atactttgaa tcaaaccaaa
    12421 taaaattgac cctaaacttg ccattattat tccaagactg ctaatttttc ttgctccata
    12481 tttatcaatc aaaattcctg caggtatttg cataaatgca tatgcatata ggcaaaagga
    12541 tgctatattt gaaatttgta tagaattaaa acctaactca tttgctaaat tgtctgaaac
    12601 aacagcagtt gacatcctta aaaataatac taaaacaaat gaaaatgtaa ttatacccca
    12661 aacactccac ttagcctttg tataactttt catatcttct ccctcaaatc catatatttt
    12721 taaattttct tgatataact tatataaagt gtatagttat tcttacaaat ttttacacgc
    12781 aatatcatta tacaacatat gtatatattt aactagaaat aaaacactat tcatattata
    12841 tacatgaatt aactacataa ataataagag ccatctttta acattactca aaagatagct
    12901 ccatataaaa acatttattc taaatttaat tttttatcta atataaattt tgtagcgaaa
    12961 aataacgcta ttgcaactac aagactacca agtattgcaa gcataaaact aggactctga
    13021 taaagatgat ataccatatt tgtatcttca tttggtaaga tgcttccaac tgcatctcca
    13081 acaattgata ttactatatt tatagctata aaagaagcaa atgccactat gtttctatgt
    13141 ttattgaatt ttggcaattg tcccattgag attgatgtat ataataacaa aataaatgat
    13201 gaatatgaaa taaacattac tactactaat aataaaatat ctaaaaagtt ttcagaaatc
    13261 catttaaaac tagtgttaaa caaatctaaa atttcaggta gtaaaattcc agaagtacca
    13321 aaaagcataa gaactccaaa agtgaatact gcaactataa aactaagtac agcgtatata
    13381 agtgctgtta tacatttaga taatataagt gaactagtgc taacaggaag agtaaacatt
    13441 aaataacctt catcccccaa aagatttttt ctaaaccttt gaattgtaac tacaatagtt
    13501 aaaactccta atgccataaa aagacttgtt aatactagta ttcctattcc ctgtacctga
    13561 agtatatttg tattcataaa tattccatta aatacagcta ctatcaatat ggctatatat
    13621 aacggtataa atattcttcc acttgctttt aactcatatt ttaataattt acctaacatt
    13681 taaattcctc cctaaataat gcatctatag attttccttt ttcttctctt attgcttcaa
    13741 cgtctccttg taacactatt tctccttttg atatgaatat tatctcatca catatgtttt
    13801 ctatttcact tattaagtgg gtagcaatca gtaaagtact atcttcagaa taatttttta
    13861 atatagtctt aagtatatat gaccttgcag ctgggtcaac accacctata ggttcatcca
    13921 atatatatat gctcgcattt cttgacatta ctaaaataag ttgaactttt tctttagttc
    13981 ctttagacat tgtctttagc ttttcattta catcaatatc taagctttta atcatctcat
    14041 ttgctcttct aacatcaaaa tcactataaa aatcatagaa aaacttaagt aaatctgata
    14101 ccttcatcca atcatttaaa taagttcttt ctggtaaata tgaaactatt tttttagtat
    14161 ctatggatgg tttcattcca tttatcataa tttcaccatt atcaggctgt aataatccat
    14221 tcattagctt aatcattgta cttttgccac ttccattagg tcctaacagc ccgactattt
    14281 ttccctttgg tatatttaaa tcaatatttt ttaaaacatt cttagttcca taactcttgt
    14341 ttacaccttt aaattcaact attccatctt gagaaacatt gcttatattt ttatccattt
    14401 tataattctc ctttcagatt ttctgtaata agttttagca tttcatctct atcatatcca
    14461 agcttttcta aggttacttt taatgtattt atctccacat tagctatttc ctctttcata
    14521 gcttttattt tttccttatc atctgttaca aatcttcctt tagttctctg actataaact
    14581 aaaccttgtc tttcaagttc tgttagagcc ttttgcattg tatttggatt aacttccgca
    14641 tcttctgcta gtgctctcac tgtttctaat ttagatccta ttttaatttc ccctgaaatt
    14701 atttttaact ttaaatgttc tactaattgt atatatatag gtttgttatt atcaagttcc
    14761 cattccatgt tcatgcctcc ttattatatt attgtattaa gttgttaata caataatata
    14821 ataatacaaa gtatatgtca attacttttt agtaaaagta taaaaaattt tacaaaaaaa
    14881 atgaacacta cattagagtc catttttaac acaatttatt aaattcttta tattacagat
    14941 taattaccct cttaccaagt tagatacaat taaatgttca aattcttcaa ttggcattgg
    15001 ctttccaaac aaatatcctt gtgccatatc acactttact tcttttaaaa gtttaaactg
    15061 ttcttctgtc tcaactcctt ctgatatgac tttcataccc aactcttttg ccatttctat
    15121 tactttagta acaattattt tgcttctttt actattataa gtttccataa taaatttttg
    15181 gtcaagtttt aagatatcca taggaatttc ttttaacata tttaatgatg agtaccctga
    15241 accaaaatca tccatagata ttaaaaatcc tatttttttt aaattattca ttatatcaat
    15301 taaaatatca aaattatcaa atatgatact ctccgttaat tcaagttcta tatactttgc
    15361 aggtatattg tatttatcaa ttatattttt gtatgtttct ataaaattat tgcaatataa
    15421 atgtatcctt gaaacattaa ctgaaacagt aactggattt attcctgcat caatccattt
    15481 tctaaggtat atacatactt cttcaagcac atataaatct aaatttataa caaaaccgtt
    15541 tctttcaaaa aatgggataa attcatttgg aggtattagc cctttatcag gacgtttcca
    15601 cctaacaagt gcttctgaac cttgtatctc tcctgtatta agttctatct taggttgtaa
    15661 gtatataata aattctttat tttctagact tgaaaacatt gtattttcca tctctttttc
    15721 ttttatctct tggtcatgaa tcttattatc ataaaatgca tatgaacttt tatgtcctcc
    15781 ctttgccatt ttatgaggta tattagccct atctatgatt gagttgatat ctctatcttc
    15841 tggaagtata aaatagattc cacaacttaa aactaactta tatttttctt cttgattatt
    15901 attaaacaag tctaattctt caaatatagt ttttaacctc tttgttatat cttcttcttc
    15961 tatatatttc ataattataa taaaattatc cgcagatatt cttgcaaaca tttcctcttc
    16021 aaatatattg ctcttaataa tctttgttaa attacgcaaa atttcattcc caacttcata
    16081 tccaaataaa tcatttatat atttaaattt atcaatatct atatataata aagcatactt
    16141 tacttgagta tttttacata gtattttgtt agcatcaatt acaaatttat ctatagtatt
    16201 tgctcctgta agattatctg tatatgctat cctttgcaat ttaattttaa aatttaattt
    16261 atcatatatt atatagaata taagtattac acatattata gccataataa gtattatttg
    16321 tattgcatgt ttgctaataa gctgattatt agacacaata gactcaaagt tatttatgga
    16381 gttgttttta tagagtttgc ataaagaata gactggtgtt gtgtatgtaa tttgtaaaat
    16441 tatacaaata aataaaaaaa acatcaattt tttttgtaaa ttcctcataa aaatttcctc
    16501 ccattaaaac acctctattt tattagcaaa taagtacatc tcaaaagttt ccatttcttg
    16561 taaaatggat agttttgaaa agaaaatatt aaaattataa tataaaaata aaatttttca
    16621 gacaatttaa acaaaaataa tcacctttta ataaataatt atatgccaaa ataatccaat
    16681 ttattttcta ttatacctat atgaaatata taaatcaata gttaagacaa tgaatatgct
    16741 acattctata attatagtag cagaaaattt tacaaaatta aagtagatat aaactatcca
    16801 tttttacaag aaatgactac tttttattaa aatacagtat ttgcaacata ttcacacttt
    16861 attttttcat ttttttctta ttacaatggt ttcgacaaat cagtaattaa tttttataaa
    16921 tgaataaatc aaataattcc t
//
LOCUS       DAMJZE010000084        16722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJZE010000084 DAMJZE010000000
VERSION     DAMJZE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.085"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            181..2529
                     /locus_tag="PC541_002258"
     CDS             181..2529
                     /locus_tag="PC541_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2839721.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLTDLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSSS
                     TNEESSEPTSTPSKEKLTGVDRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDNNKEVNNTLPSVT
                     KIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVV
                     LVGNGLDNSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
     gene            complement(2571..2756)
                     /locus_tag="PC541_002259"
     CDS             complement(2571..2756)
                     /locus_tag="PC541_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2839722.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(3274..3450)
                     /locus_tag="PC541_002260"
     CDS             complement(3274..3450)
                     /locus_tag="PC541_002260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839723.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            4167..4844
                     /locus_tag="PC541_002261"
     CDS             4167..4844
                     /locus_tag="PC541_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2839724.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            5013..7049
                     /locus_tag="PC541_002262"
     CDS             5013..7049
                     /locus_tag="PC541_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2839725.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(7240..7806)
                     /locus_tag="PC541_002263"
     CDS             complement(7240..7806)
                     /locus_tag="PC541_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2839726.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            8094..9029
                     /locus_tag="PC541_002264"
     CDS             8094..9029
                     /locus_tag="PC541_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2839727.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            9494..10708
                     /locus_tag="PC541_002265"
     CDS             9494..10708
                     /locus_tag="PC541_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839728.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(11128..11649)
                     /locus_tag="PC541_002266"
     CDS             complement(11128..11649)
                     /locus_tag="PC541_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2839729.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            12005..12802
                     /locus_tag="PC541_002267"
     CDS             12005..12802
                     /locus_tag="PC541_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2839730.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            12912..13502
                     /locus_tag="PC541_002268"
     CDS             12912..13502
                     /locus_tag="PC541_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2839731.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            13568..15265
                     /locus_tag="PC541_002269"
     CDS             13568..15265
                     /locus_tag="PC541_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2839732.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            15479..16318
                     /locus_tag="PC541_002270"
     CDS             15479..16318
                     /locus_tag="PC541_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2839733.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         6692 a         1744 c         2653 g         5633 t
ORIGIN      
        1 gcattagatt ttattaatag ataaattaaa aagtagcaac taaaagtagt tgttatcttc
       61 taatgcctca ttcataataa aaaaataatt tattatataa taatattaaa gtttgtttct
      121 aaggttaata ttatgtgata caattcaagt gggtaaatat tatttgaatg aggtgtgggg
      181 atgaaaaatt taagcaaaaa agcagttatt ttatcacttt cattggtagt tgtatcacct
      241 ttggtacata agataaatgc acaagatatt gagaaaaata gggaagttag atatgaaaca
      301 aggaaagcta ataacttaga aaatatgaca atggaagaag cttttccaga tgaaaatttt
      361 agaaaagtaa tttgtgataa actaagtatt ttagatgatg gaaatccaat tggaatttca
      421 caaaaagcga ttatagaatc tacaaaaggt ttaaccttag ggagcaaatc aataaaaaat
      481 ttatctggaa ttaattattt tattggatta gaaaatttta gttgtagagg aaataattta
      541 acgagtctag atttaagtag caacataaat ttaaaagaat tgtattgtaa cgaaaatcaa
      601 ttaacaagtc tagatttaag taataataga gagttaacaa cgttgcattg tggagaaaat
      661 aatctaacag acttagttat agaaaattct aaattagacg aattgaactg tagaaaaaat
      721 aatttaaaga gtttagatat aactaaagca actaatttga caactctttt atgttttgaa
      781 aatgaattga ctagtttgaa cttatctaag aatcagaaat taaaaatttt gaaatgtgat
      841 tataataatt taaatgattt agatatatct acaaatttag aattacagga tttaaactgt
      901 tcttttaatc aattagaaaa tataaattta gataataata aagaacttgt taagttaatt
      961 tgtcacaaca ataaattgca aaatttaaat ttaaaaaatc atgaaaagct tgtaaaacta
     1021 tattgtaatc aagaacaatt aagtacttta aatgtggaaa attgtattaa tttggaagat
     1081 ttaacttgta gtagaagtaa tctgaagaat ttggatataa gtagcaataa gaatttaaaa
     1141 tatctagagt gtgtagcaaa caagttaaca aatttgaata tagagaataa tatagaactt
     1201 ttagaattat actgttatga caatgaaata aaagctttag atataagtaa aaatattgac
     1261 ttagaaaatt taacttgtta taaaaataaa ttagatagtt taaatacaag taaaaataaa
     1321 aatcttaaat acttatattg ttcacaaaat gaattaacta gcttggatgt aacaaaaaat
     1381 acagaacttg taaggctata ttgtggggaa aatagattaa ccagcttaga tgtaagcaaa
     1441 aacacaaaat taaaagaatt agattggtca aatcaaaaaa aatcaacatc aacaggaggt
     1501 tcgtcaggga gtggaggagg ttcatcatct acaaatgaag agtcctctga acctacttca
     1561 acaccatcta aggaaaaatt aacaggtgta gacagaaatg aaacatcagt gaaaataagt
     1621 caaaaaggat ggaataaagc tgataatata gtgttaataa atgactctag catatcggat
     1681 gctttatcag caacaccatt tgctaagtct aaagatgcac caatattact tactaaaaat
     1741 aataacttaa ataaattaac agaaaaagaa ataaatagat tagaagcaaa aaatgtgtac
     1801 atagtgggcg gattaaagtc tgttgatgaa aaagtagtgt ctgatttaaa gaaaaaagga
     1861 ttaaatgtaa ttagaatatc tggaaatgat agatatgaaa catcaataaa acttgcaaaa
     1921 gaattagaca aaaattctaa cctgtcaaaa gtagtagtgg taaatggaga aaagggacta
     1981 gctgatgcag taagtatggg agctatatca gcaaaagagg aaatgccaat tcttcttact
     2041 aatcaaaatg atgatatgaa agatataaaa gacttaatag ctaataaaaa catatcaaaa
     2101 tcatatgtaa taggtggaga atctttattt aataataaag aagtgaataa tacattacca
     2161 tctgtaacta aaatagcagg ttctgataat aataaagaag tgaataatac attaccatct
     2221 gtaactaaaa tagcaggttc tgatagaaca gaaactaact caaaagtaat aagtcatttc
     2281 tatagcaaag atacacttaa tgacttatat gtagctaaga atggaatgaa taaacaagac
     2341 gatttagtag atgccttatc agtaggagtg cttgcaggta aaactgaatc tccagtagta
     2401 ttagttggaa atggattgga taatagccaa aaagagttaa tcaagaataa aaagtttaaa
     2461 aatataactc aaattggtgg aaatggaaat gaaaaagcat ttaatgaagt agaaaactta
     2521 gttaaataga ttgaaaggta gtaactggaa atagttacta cctccttttt ttattcattt
     2581 tcagtttttt caataattat ttttccatcc tcaaaagtaa caagaacatc cctttcatca
     2641 ggagagatat tcatctcttt aatccatgac attggaagta tcaatctggg tgtaaaagag
     2701 cctttgccac ttttattaaa tccaattcta agttttcttt gctccaataa tatcattcct
     2761 ttctactatt aataaatgcc agcaataata aagtaaatac caaagcaata agagctttaa
     2821 aagtaaaact tattattata ttaataatat ttaaaactat agtgatacca agaataatca
     2881 tacaaattct ataaaattta ttattcatat taaattagaa aatgtgttat aatattagaa
     2941 agaagctacc tagaaggaag gtattttcct cctaggattg aaacttataa ttctttaatc
     3001 aatcttagta ttgcgagtac tagattaatt aaaactaatg tgaaagagaa agcattttta
     3061 attttattaa atttgttttc tcttttattt tttcttcttt ctaatctccc cactttctta
     3121 cctcctttct atatttatat tatatcatca cgtgacgtga aagccaataa atattccata
     3181 tttttcaata aagttataaa atatattaat ataaaataca aaatataata aaaataagtg
     3241 gatgtttaca ttatacaccc acttatttta gagttattcc tcatcagatt tttttattat
     3301 aatctcatct tccttaaaaa taactacagc cttacgtttt tcccttgtaa atcccatttt
     3361 ttcaagttgg cttaaaggta aagaaatttt agccgaataa gtacctgaac cacctttact
     3421 gtaaactatt ttagattctt tttgctccaa actattttcc tcctttttta tattgtaatg
     3481 taacaaatat taacaataaa atgcttacta ttaatcctat tatattgttg atattaaaat
     3541 gtaaaaataa aagtattatg tctattacta tagttatagc aactataata agacagaatt
     3601 tataaacttt atcactcata ttttatagaa aatgtgttat aatatttaga aagaagtcac
     3661 taggaagaag attaaattct tcctagctta caaaactata aatcttttaa taatttaatt
     3721 agtttatcaa ttaaatttat taaaatgggt aagatagaga gagcagtttt aactttggcg
     3781 agtttgcttt ctctatcttt ttttcttctt tctattctac tcattttcct tttcaccccc
     3841 tttctatatt tatattataa catgccgaac cggcgaaaac aatatttatt tccatatttt
     3901 tcaataaagt tatattgata aactatattt tttacaaata aaaataaatg gtgacaaaac
     3961 caaacttgcc acccttttgc caccgaaaat aaataataga ataaatttaa attaaacata
     4021 ataaaacata aacaacaaaa ctgaccttaa taaaacattg aaataccaac acttgtaaaa
     4081 aataacaaca ataaacaaat aacctagaat aatattacaa cttaagtaaa taataatttt
     4141 gaataataat ttacggaggt attcatatgt ttaaacacga taaagcatta ttaaaagaag
     4201 taaaagtaga aagaccaaat cctcaatatg ctgttttaat gcaagaacag ttgggtggtg
     4261 caaatggaga gttaaaagca gctatgcaat acttgtccca aagttttaga ataaaagacc
     4321 ctcaaataaa agatttattt cttgatatag cagcagaaga gcttagccat atggaaatgg
     4381 tagcacaaac tataaattta ttaaatggac atgatgttga ttataatgca gttaatacag
     4441 gcgaaataga aacacatgta ttaactggtc tatcaccagt cttaataaac tcatcaggag
     4501 ctccttggac tgctaattac gttacagtta caggggattt agtagctgac ttactttcaa
     4561 atatagcttc tgaacaaaga gctaaggtag tttatgagta cttgtataga caaatagatg
     4621 ataaatatgt aaaagaaaca atagatttct tacttaatag agaagaagca cataatgctc
     4681 tatttagaga tgctttaaat aaagttaaag atacaggttc aaatagagat tttggagtta
     4741 cagaagattc taaattatat ttcgatttgt ctactccagg gccaaatcac gatactaaga
     4801 tagatattaa tcctccttct tttgaaaaac ctttaaaaaa ataatttgat attttttatt
     4861 ttgttttagt agttataaaa attatttact agaagaatta attatatgaa aaattaaata
     4921 tgtatagtta aaaatgggta tacacttatt ataagtgtat atctatttgt ttattgcaaa
     4981 acatataata aaataacaaa tgaggtggta ttttggacag taatagaaaa cctaataatc
     5041 taataaatga aaaatctcca tatcttttac agcatgctta taatcctata aactggtata
     5101 gttggaatga tgaggctttt aaaaaagcaa aagaagagga taaaccaata tttttaagtg
     5161 taggatattc aacatgtcat tggtgccatg ttatggaaaa agaatctttt gaagatgaag
     5221 aagttgctga aataatgaat agaaactttg tggcaataaa agtagataaa gaggaaagac
     5281 ctgatgtaga tagtgtgtat atgacagttt gccaagctat gacaggtagt ggtggatggc
     5341 caatgaccat aataatgaca ccagataaaa aacctttttt tgcgggaaca tatttcccaa
     5401 aatattcaag gtataataga cctggagtta tagatttatt agagaatgta tctgaaaagt
     5461 ggaatacaag cagagatata ttaataaaat ctggagatga aataatagaa gctcttaagg
     5521 atgattttgg tgtaaaaaat actgagggtg atttatcaaa agagatgttg agttcatcag
     5581 ttagagtatt taaggctatt tatgatgaaa agtatggtgg ttttggtaat gctcctaaat
     5641 ttccaagtcc tcagaatctt atgtttttaa tgaaatatta tagtatagaa aaagacaaag
     5701 atgtgttaaa aatggtggaa aaaactttag atggaatgta tagaggaggc ttgtttgatc
     5761 atattggatt tgggttttca agatactcta ctgataaaaa gtggttggct cctcattttg
     5821 aaaaaatgct atatgataat gctatgctta ctatagcttt tttagatgcg tataaaataa
     5881 caaagaaaga gttgtataaa gaaattgcta taaaaactat agattatgta gttagagaga
     5941 tgaaggataa agaaggtggg ttttattcag cacaggatgc tgatagtgaa ggtgaagaag
     6001 gtaaatttta tacatttaat cctcttgaga taattgaagt tttgggtgaa gaagatggaa
     6061 ttttctttaa taattatttt gatataacta gtagtggaaa ttttgaaggc aaaagtattc
     6121 ctaatcttat aaaaaataaa gaatatgaga gacataatga aaagatagct gaccttagta
     6181 aaaaggtatt tgaatataga aaagaaagaa catctttgca taaagatgac aagatactta
     6241 catcatggaa tgcattgatg atagttgctt taacaaaggc atatagtact ttaaaaaatg
     6301 acatatattt agagtattca aataagtgtc ttaactttat aaataataat cttgtaaatg
     6361 agtcaggtag attattagct agatatagag atggaagttc cgactattta gcatatttag
     6421 atgattatgc ttttttaatc tgggcgtata tagagcttta tgagtcaaca tttaatatga
     6481 aatatttaga aaaggcttta aatttgaatg aaagctgtat caaccttttt tgggattatg
     6541 aaaaaagtgg attttatata tatggtaaag atagtgaaaa tttaatagca aggccaaaag
     6601 atttgtatga tggagctatt ccatcaggaa attctgttca attatacaat cttataagac
     6661 ttgcgaaaat tactggagat aatagacttg aagaaatgtc atataaacaa ttaaaactgt
     6721 atgtggataa tgtaaagagt tctccagctg gctacagctt ttatatgctt tcattgatgt
     6781 ttgaacttta ttctactaag gaaattatat gtatttttaa ggaagattct gatttaattg
     6841 cctttaaaga attgataagt gaaaatttta taccaaatgc aactttttta gcaaagaaat
     6901 acaatgaaga aaacactatt ataggttttt taaataacta tagattgaag gatgataaga
     6961 ttagttatta cgtgtgtcaa agtaatagtt gtagtcaacc aattaatgat ttacaaaaat
     7021 taaaagatat gattttagga gtagaataat cataatagtg aaaaaggaga tgctttaaaa
     7081 taaaagtcta atcatctcct ttttttgtac aaaaaacaga aaaagagttc ctgagaacac
     7141 ctaatctatg taatgattgt tttgttaaaa aataaaaatg acattatagt tatataaaat
     7201 attgtactat aaaaaagtag tattatagat gcgatagtat taatcgtttt tatttactct
     7261 ctttcttctt tttgcaaatt cttcaagtgc ttcagattta attttatcta tttcttcata
     7321 ttccataaaa ttagcatctt cttcaaagcc atcttgttct tcaccattaa aacgatacat
     7381 tttttttagt aaactccata aggtctctaa ttcatctttg gtaaattcgt gaaatacatc
     7441 tgccataaag tttattccag tcctactaca tgttaccata accttttttc ctaagtctgt
     7501 aactttaaca ttaattgctc tcttatcatg aggacttgga attacatcaa catatccatt
     7561 tttttcaaga ttagcaacta gtctatttat attttgttta cttgtaccca tttttcttgc
     7621 aatattattt aatgttgttt cttcctctgg taaatgaaga attgataaaa tagtcatata
     7681 ctgtcttgaa gtaagtatac caaaatattt atctccttct atttgtattt tgtttgtaag
     7741 ggtaaaaaga gttgcatatg tttgattcat taaaaagagt tcttttagtt catttgaata
     7801 atccatgaaa tatatctcct ttatttttag atgttgtttt gtcgaaaaca gaatatcatg
     7861 aattaattct aatgtcaata tggtgtcaaa atatattaaa gtattacttt tttatgtaac
     7921 tattaattaa ttaaattatt gtataatttt atgtaaaaac ttctatgtac caatgataac
     7981 ttctatgagg aattaataat tcttaaattt tttagaaaga ttttgacttg aaaatacttg
     8041 taatataata tataaattaa tatactgaat aaaatcaatc tggagggata attatgaagt
     8101 gtacaaactt tactttcaaa ggagaggaag gtttagatat atatacatat aaatgggaag
     8161 atgaaaatat aaaaaaacca aaggcagtta tccaaattgc acatggaatg gctgaaactg
     8221 cacaaaggta tgagactttt gccaaggtac ttactaaaaa tggttatata gtatatatca
     8281 atgaccatag aggtcatggg aaaactgcaa agataataga aaatgttgga catttagctg
     8341 aaaaagaagg atttagatgt cttgtagagg acatgtatac tttaacaaat attataaaga
     8401 aagaaaatga agatctgcca atttatctat ttggacacag catgggttca tttgcaagtc
     8461 aaagatatat aatggattac agtaataatt tatctggtct tatactatgt ggttcaaatg
     8521 gaaagcaagg aatcattcta aatcttgctc atctgataat caatcgtgag attaaaaagt
     8581 atggaagacg ttctaaaagt aataaaataa ataatttgat atttggcggc gaaataataa
     8641 gaagaaatga aaagactaag tttgactggt taagtagaga taaagaacaa gttgaaaaat
     8701 atataaatga cccattttgt ggagtagtgt gtagttgtgg ctttttttat gatttagttc
     8761 aagggttaaa agaaattgag gataaagaaa acttaaaaaa agtgccactt gatataccta
     8821 tttacataat atctggagat aaagacccca taggaaaaaa tggtaaagga gtcttaagac
     8881 taagggatag atatataaaa ttaggagtaa aagatgtaac ttgcaaactt tacaaagatg
     8941 gaaggcatga attgttgaat gaaataaata gagaagaagt ttttgaggat ataatttgtt
     9001 ggttaaataa taaaatagaa atattgtaat aaaaaaatat aatgatatat ttactttaaa
     9061 tttatttata tataatattt gaaaagtgga ttatgtatat gcaaagtaga aaagagagag
     9121 cccagaagtg attttttgct ataatgccat ttattgaata ggtgggtgtt attataaata
     9181 aattattaac actcagtatt attatcattg tatatggaat ttattttatt taaaattcct
     9241 tgaaagttta aatatagtaa gttgtagtaa agagaaaata taaatattta aatgctattt
     9301 aatgtgaatc tattaattag gttatactta aacttaatta atagatttta tatttcaaga
     9361 caatgagata gggttttaga ttaaaatata accaatggtt aaattacaga cattttgtta
     9421 actttccctc gatttactag taaatatttt ttttatatac ttaaattaca acagatgtac
     9481 tagggaggta tatatgcaaa ttaaaatagg taaagtcata cagtgtctta gaaaagaacg
     9541 aaatttaact caagaacaat tagcaaagtt tataggtgtg tcaacaccag cagtatctaa
     9601 gtgggagagt ggaaattctt acccagatat agaattattg cctttattag cagacttttt
     9661 taatgtgtct attgataagc ttttaaatta caagattgat ttaagtgaag aagaagtaat
     9721 gaaaatttat aaagaattag aatcaggctt tgctagaatt gaaattgatt tatcaacaga
     9781 agaaccaaaa gaagaattta gacaagattt agaatctgta aaaaagcttt ccaatatgta
     9841 tatagagaag tatcctaaaa gctatttatt gaagctaagg atatgttcgc tgtatcagat
     9901 gtattcatat aaatttggta agagtgaact taatgacaga gtaaaagaaa caacgaatat
     9961 tcttgaagat atagtgagaa atacagatga tatccaaata aaagaaacag ctcttattat
    10021 attatcaaac gcatattgta tgttggagga ttatgaaaag gcagagttgt atttaaatat
    10081 gatacataag tcaatagggg atacaagtgt taatttagct atgatatatt taaagcaaaa
    10141 tagacttgaa gaagcagaaa tattgcttca aaataaattg tttagtaatg tatttaatat
    10201 aagcatggat tgtaagggaa taattaatgt atacaaaaat caatacaagg aattaaagaa
    10261 aaaattggag aatagaaatt ttaataaaaa tgcaattgag agagaaatgg agtatataaa
    10321 aaataagttg ctggggtatg caaatctttc tttagaaata aaaaaaatgc tcagtgaaga
    10381 taaaggtgca ttttttagta tgtatatgga ttatatggaa ttatctctaa tttttttatt
    10441 ttttaacatg aaagaagaag caaaaaaagc tttatatagt ctaaaagaaa tattagaaaa
    10501 atatcctata catgaaagtt tggatgtaag tcaaatgaga ttttttgata aggtagagtc
    10561 taaaaattta tatactttta atatatatac aaacttgtta attgtactta atgatgacag
    10621 ttataatgag cttagagaag aaccaatttt taaggatgta attgaaaaga ttttagatat
    10681 ggaaaaaatg ttgaaaaata aagaataagt aaaaatttaa tataggactt aaagattaat
    10741 caaattaaaa gaaagtgttt caaaataaaa atttgaatca ccttttttgt cggtcttaaa
    10801 cagttattta gaaaaataga ggtttcaaaa tagaaatctg aattatctgt attattacac
    10861 aaaaatagaa acctaaattt ataaaaatag aataaaattt gttatataaa atgtaatttt
    10921 gtctataaaa tctcacctgt tttcttaact ttaagcgtga aaaagaggat gtatcatgaa
    10981 ttttttagtt catgatgcat cctgctttgt atgaaatcca atatatcttt attatttaaa
    11041 caatggaaaa gagtcttatt tatattttga gacatctctt ttatttaatt atgaagtttt
    11101 atacagttca ataaatagga attatattta gaaactcatg acattatttc ttagatagtg
    11161 attaaattta tcatattcca ttttagataa cttttctggg taagacatta caatcaggtt
    11221 atgcccatta aattttttat ggtatggtgg atgaatgtgt tcataattat ttgtcttata
    11281 ttttactctt tcatagaacg ccttgcgata aattgaaatc tcatcaattg gaaggtcaat
    11341 ttctaaaata atagtttttc ctttcttatt taaaagctct aacgctcttt gcccatatct
    11401 attattgcga atttctggaa aaatgcagaa atgttctaca tagataaagt tatctgtttc
    11461 ccaacataaa attatgccga caaactggtt tttatcataa attaaattga attgatattc
    11521 tttatttttc aaaatttctg cttgcaaaga agattttctt tgtttatgaa atgggaaact
    11581 aattttatat aattccatag ccttgtaata taatttattt tcactggttc ttaaccgttc
    11641 aaatttcact ttactcctcc taatcacgaa aattccaatt tatttatgag ttcattttac
    11701 catagtcagt atttgcatac aatgtgtttt aagttgagtg tcatttcttt cttttattta
    11761 tgcttatatc tcatagtcta agagataatt gcttaagaca taactaaaaa ctattatatt
    11821 aagctttgat aatctaaaaa cagtatattt atggactttt ctaggtctaa aagtaatctt
    11881 aataatttta gaagttacaa aaaagttaca taaatttgga aagctataat atacatatat
    11941 aatataaggt taaaactgta atgaataata ttaatttaaa atttgaacat ggaaggagat
    12001 tattatggag aaaagagaaa agaggataat aatatcatct ttattaagtg tatcaatttt
    12061 aatgggtttg gcaagtatat attctatatt aaataaagaa gacataagtt taaccgttaa
    12121 aggtcaagag caaaaagtat cttcctttaa gaaaacagtt gaggaacttt tggatgaaca
    12181 gggtgtaaag tataattctg aagataagat taatccaagt ttagatacag agttaaaaga
    12241 tgatatgaaa ataaaagttg ttaaggtaac taaaagtaaa aaagaagaga ttgaaaaaat
    12301 tccatttgat acaaagcatg taaatgatag taatttgtta aaaggaaaat ctaaagttta
    12361 tcaagaaggt caagaaggag aaaaaaaact agtctataat ttaacttacc atgatggcaa
    12421 gttagtcaaa aaagtcttat caaaagaagt aatatctaag gagccaacta caaaaattat
    12481 aaaatatgga actaaagaaa aagtactaat agcatcaaga ggagcaaata taagaggagg
    12541 caaacatatg aaagtggttg ccactgcata tgcaggagat acaataacat ctactggtac
    12601 aactccaaga tggggtgtta ttgcagttga cccacgtgta ataccatatg gaacaaaagt
    12661 atatatacct aaactgggta tgacttttgt agcagaagat tgtggaggtg caattaaagg
    12721 taatagaata gacattttta tgaacagtga aggaaaagct tcaaattggg gaaggaaaag
    12781 tatagatata tatctacatt aaattagtga atagaataac tttacttgaa ggacaaaaaa
    12841 gtataaaata tgaattaact aaactgttag attattctta aaacataaaa atatatctaa
    12901 ggggggataa gatgaaaata gtctgcttag gagacagttt aacgtatgga tttggagtat
    12961 cacgaagtaa ttcatggaca aacattgtaa ataaagagac tcgattagaa atagtaaata
    13021 agggtataaa tggtgataca acaagtggta tgttagctag gtttaatgaa gatgttgtaa
    13081 aaaactctcc tgatatagtt tttataatgg gtggcaccaa tgattttatt gctggtgcgg
    13141 gaaatgaaat tattaattct aatataatgg ctatggtgca tcagtcatat tcaaaaaata
    13201 ttatacctat aataggtata ccattaaaac cagatattcc aaatgttagg gaagattgga
    13261 gttgttttac tgattttaat atagtatctc aaaacttaga atcatatagt tattggataa
    13321 aaaaattttg tatgacattt aatactaatt tcgtagattt ttattcagaa tataataaaa
    13381 atatggaact agaaggatat aaaaaattat attttgatgg gttacatcca accaaagagg
    13441 gtcatagaat aatggcagac atttttatta attcaattaa taagtatata gaagagttat
    13501 agattacatt aaaatgcttg tttatgcgtc agttgcaata aatacataca gtagagaggg
    13561 gtttattatg gattatacta aaacttatga agagtggata aagggttcat attttgatga
    13621 agatacaaag ctagagttgg agaatataaa aaataatgaa aaagaaatag aggatagatt
    13681 ctataaggat ttagaatttg ggactgcggg tcttagagga ataattgaag caggaacgaa
    13741 tagaataaat aagtacacag ttagaagagc tacttttgga ttggcaaact atatattaga
    13801 aaacacaaca aaagaagaaa caagtagagg agtagtaata gctcatgata atagacacaa
    13861 gtctagacaa ttctgtatag aatctgcaaa tactttggca gcatgtggta taaaagcata
    13921 tatatttgat agtttgagaa ctactccaga attatctttt gctgttagaa gcttaaatgc
    13981 aatcgctggt atagtcataa cagcaagcca taatcctcca gaatataatg gatataaggt
    14041 atactgggaa gatggagcac aagttatgcc agagattgca aatgcaataa cagagaaagt
    14101 aaatagtata catgattata gtacaatacc aaccttaacg gaagctaata aaaatttggt
    14161 agttttgttg gacgaatcac aagatactaa atttatagaa gcagtaaaaa gccaaattat
    14221 aagaaaagat ttagttaaaa atgtaggtaa aagctttaag atagtttata cacctctttg
    14281 tggaacaggg aatgttccaa taagaagagc attgaaagaa gttggttttg aaaatataat
    14341 tgtagttcca gaagaagaaa atccagaccc aaattttgca gggcttgatt atccaaatcc
    14401 agaagagaaa aaagctttaa atagaggtat attactcgca aaagaaaaag gagcagacct
    14461 tgtaatagca actgacccag attgtgatag agtaggtgta gcagtaaaaa ctactacagg
    14521 tgagtatgct ttacttacag gaaatcaaat tggtggtatg ctgacacatt atataataga
    14581 aggattaaaa gaaaataata aattaaaaga aaatcctaca ataataaaaa ccatagttac
    14641 atcagaattt ggagcagata tagctaaagc aaataatgtt gatgtattaa atgttcttac
    14701 aggttttaaa tttattggtg aaaaaataaa attatttgaa caaaataaaa atagaagtta
    14761 tgtatttgga tatgaagaaa gttatggtta cttagttggg actcatgcta gagataaaga
    14821 tggtgtagtt tcttcattgt taatatctga aatggcagca ttttactatt caaaaggtat
    14881 gagtttatat gatggcttaa tagaacttta taaaaagtat ggatttttca aagaacagac
    14941 tatatctttg actttaaaag gtatagaagg tgtagaaaag ataaaagaaa taatatctta
    15001 ttttagagaa aatcaaattg attatataaa tagtattaaa gtagttgata aaaaagatta
    15061 taaaaatggt atagataatc ttcctaaatc tgatgtttta aaatactttt tggaagatga
    15121 atcttgggta gctataagac catctggaac agaaccaaaa cttaaatttt acatagctgt
    15181 aaaaggagct agtgatattg aagcagataa aaagattcaa ggtttgaaaa agtatataga
    15241 tgacatggtc gaaaaattaa aataaaatat tattatttta taagataatt ttgtaataat
    15301 atttatttag ctgaaaatta taatttactt tcattgagta gaatatggaa atgttagtag
    15361 aattgttatt ttcagctttt atttgtgtta tttttactta aataattata ttatagaatg
    15421 aatctgagta taattaatat tgtataatga aattaatttg atagaaaagg ggatatttat
    15481 gtttggtata gataagaaat tgattgaata tataattgca aaatccaatt acagtgaatt
    15541 aagttcaaag tcagcagaag tatctttctt cttaatgttg tccatatttc cttttttaat
    15601 atttacaata agttctatag cttatatacc gattcttcat ttaaataagt atatagcttt
    15661 gtttagaaat atgatgccag aaggtgcttt tgctgtgtta tcttcaatca tagtttctgc
    15721 aatagacaat agaaacctga aatttttagc agtcagtttt gtacttacta tgtggacatt
    15781 ttcaagagca gtaaaagctc ttattaaagg aatgaataga gcctataaag ttaaagaaac
    15841 taggtctttt tttaaaattt tatctatatc atttttattt acaataatgc ttttggtcct
    15901 tattttttta tctatgatat ttttagttta tggggaaaaa ataggttatt ttatttttaa
    15961 tcttgtagga ttggatgaga tatttataaa aatatgggat attctaagat acactgtagg
    16021 tataataaca ataatagtga tatttacact tttatataaa tacactccaa ataaaaagtt
    16081 aactattaaa gaatctgcgc caggtgctat ttttgctact tttgcttggt ttttagtatc
    16141 atttttatat tcttattata caaattacta tgctaactat gaagtaatct atggaagtat
    16201 agctgaaata attgtactta tgacctggat gtattttagt agttggtcta tcgttattgg
    16261 ctatgaggta aattcaagat tgtattttag aaagataaga catgaaatgt taaagtaaag
    16321 tatttaaaat aaaagagagt atttagtatt ataaatgtaa ttgaattaat aaagaaatat
    16381 tgacatttaa gttaaaaagt ctagaatttt tctgggcttt ttttattttt agtataactt
    16441 gacaaatttt atttaatggg atagttttaa tttataatca tctttaaaaa gatgactatt
    16501 gataagtaag agataaaatt ggtaagtatc aaaaaatata ctaaataaaa attatagtcc
    16561 taatgagtta atttctgtat tttcaaataa tgcagtattt agtcatgtgt ttttatatgc
    16621 gcctgtattt ctaattacca atatattaga ataagctact gtaattagat ttagaaaaaa
    16681 gcgacataaa taattataaa caaataataa attacataaa at
//
LOCUS       DAMJZE010000085        16634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJZE010000085 DAMJZE010000000
VERSION     DAMJZE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.086"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            118..2181
                     /locus_tag="PC541_002271"
     CDS             118..2181
                     /locus_tag="PC541_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2839734.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2178..4043
                     /locus_tag="PC541_002272"
     CDS             2178..4043
                     /locus_tag="PC541_002272"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2839735.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4167..4981
                     /locus_tag="PC541_002273"
                     /pseudo
     CDS             4167..4981
                     /locus_tag="PC541_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5006..5494
                     /locus_tag="PC541_002274"
     CDS             5006..5494
                     /locus_tag="PC541_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2839736.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5607..5768
                     /locus_tag="PC541_002275"
     CDS             5607..5768
                     /locus_tag="PC541_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839737.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            6008..6235
                     /locus_tag="PC541_002276"
     CDS             6008..6235
                     /locus_tag="PC541_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839738.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6232..9171
                     /locus_tag="PC541_002277"
     CDS             6232..9171
                     /locus_tag="PC541_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2839739.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9171..9845
                     /locus_tag="PC541_002278"
     CDS             9171..9845
                     /locus_tag="PC541_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839740.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            10006..10422
                     /locus_tag="PC541_002279"
                     /pseudo
     CDS             10006..10422
                     /locus_tag="PC541_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10382..12829
                     /locus_tag="PC541_002280"
                     /pseudo
     CDS             10382..12829
                     /locus_tag="PC541_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12969..13391
                     /locus_tag="PC541_002281"
     CDS             12969..13391
                     /locus_tag="PC541_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839741.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13929..14963
                     /locus_tag="PC541_002282"
     CDS             13929..14963
                     /locus_tag="PC541_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2839742.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15050..15253
                     /locus_tag="PC541_002283"
     CDS             15050..15253
                     /locus_tag="PC541_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2839743.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15424..16584
                     /locus_tag="PC541_002284"
     CDS             15424..16584
                     /locus_tag="PC541_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2839744.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         6796 a         1616 c         2912 g         5310 t
ORIGIN      
        1 ttttattcgt ataataatag aaatatttta gcataagatg tataataaag gaaaaaatac
       61 agaattaaaa aacataagat agataagtgg acttagggga gagaaattat tatgcaattg
      121 acaaaaataa ttcttagtaa ctataggtgt tttggagaag aacctacaac tattgaaatt
      181 aataatttaa ctggatttat aggtcataat agcagtggta aaactgcttt attaagtgca
      241 cttatgaaac tttttggaga aaagtctagt gatagaatta ttgaaagatc agatttccat
      301 atacctgaaa gtattgatcc agatagtatt tctaagaatg aattatatat tgaagcacaa
      361 tttgagtttc ctgaattgga gagaggtaaa gacagcatat ctataccagt attttttaaa
      421 aatatggttg ttagtgaacc aggtgggaaa ccttatttaa gaattagact tgaagcaaat
      481 tgggagaaaa gctcaaatcc tgaaggaagt attgattcaa atgtatacta tataacatct
      541 acaacagagg agataaatcc tgaagataag cgtaatatca aaagatatga gcttgatcaa
      601 ataaagatga tttatgtacc tgccattagg agaccagatg aacagttaaa aaatgtctca
      661 ggaacaataa tttcaagatt attaaatggt ataagttggt cagaacaaac aaaggcaaga
      721 attaaaacga aagcaagtga aacggaggaa attttctttg aagaaaaagg ggttgaatca
      781 ttaaagaaat cactcgaaac tcaatggaat agttatcata aggatattag atattctaat
      841 ccaaagataa acttcaatag tactgattta gagacaattt taaaaaaagt tgaagtaact
      901 ttttcaccta cagggacaca tcgagattat cgggttgaag agcttggaga tgggttacgt
      961 tctttatttt atctttcact tgtaaataca tttttagaaa ttgaagaaga aattctaaaa
     1021 tctattaatg atggtaaaga agaagatata tttacattaa taccgcctgt attaacaatt
     1081 gttgcggttg aagaaccaga aaatcatatt tcacctcatc ttttaggaag ggtagttgaa
     1141 aaattaaatt ttatttctac aaaagaaaat tcacagacaa tattgacatc acattcacca
     1201 gcaatagtaa aaagaattga accaacagac ataaggtatt ttaggatttg taaggaatat
     1261 gaatgtacaa aagtgaagtc aattatatta ccaaatgaag aaaaggaagc agaacaattt
     1321 aaatttgtaa aggaatcagt attagcatat cctgaacttt attttgcaaa acttgttgta
     1381 ttgggcgaag gtgatagtga agagatcatt attaagcaga tgattgaatt aaaagcagaa
     1441 tctgtagatt catcagaaat ttcaatagta ccattaggtg gtaggcatgt taatcatttt
     1501 tggcgtttat taaatgattt agaaattcca tttattactt tgttagattt agatagagaa
     1561 cgagatggag gtggttgggg acgtataaag tatgcaatac aacaattaat aaatattggt
     1621 catgataaga atgtattact ttatacatgt gaaggaattt cacaagataa tgaatttaaa
     1681 gagatgcata cttgggatgt aactgatact gaaaatatgg ctaaatggat tgaaatgcta
     1741 aaaggttatg atgtttattt ttcagctccg ttagatatag attttgcaat gttacaaagt
     1801 tttaaagata aatatatttc agcattaaca agtgcggaag gtcctaggat aaaagatcat
     1861 ggaaaaatac aagatattgt gatcaatgaa gatgctgaag atgaatcatt gaaagctgct
     1921 tataaagata gaatagatag tgatattaaa tcaaccttaa aacaagaagg tggtgatgga
     1981 tctacttata ctgagggaga gaaagaacta atgatatggt attcatattt ctttttagga
     2041 agaggtaaac caactactca tttatcttta ttttctaata atactgatat agattttgaa
     2101 agttttccat actgtctaaa ggagtttgta aatagtatta ttgaaaagat gatagatgat
     2161 tcagggggtg gaagaaaatg aagaggaatt gggttagtaa agaagattgg agtccagcgg
     2221 ctggattcag actagaagtt gaagcgatga atacagttat taatgataag aatacacttg
     2281 ttgtagctgg tcctggcgct ggaaaaacag agttattagc tcagagagcc tgctttttac
     2341 tagagactaa tacctgtaga tatcctaaaa aaatccttgc tattagtttt aaaaaggatg
     2401 ctgctgaaaa tttaaaagag cgtttagaat tgagatgtgg gaaagaatta gcttcaagat
     2461 ttgaatcaaa gacctatgat gcttttgcaa aagaaatcgt agatagattt agaaatagtt
     2521 tagatgaagc atatagacct ttaaataatt acaatatagc tgaaaatcgt gatgttagaa
     2581 gagcatttga gatagcagga ttaaatttca gaggaaatca aactgaattt aataaagtat
     2641 atggaaataa actatcgata aataaattgc ctttacatac agattttgta gataagattt
     2701 ttatagatgc ttggaatgta ctacttaaag gcaacaaaga tactaatttt gtaagttcac
     2761 ttacttttgg tatgatatca cgtttagccg aatatttgat aagaaccaat aggtatatta
     2821 agaaagcaat tgaaatgact tatagtcatg tgtttttaga tgagtttcaa gatactaaat
     2881 ctattcaata tgatcttgtt aaagcttgtt tttttaaaac agaaacaata attactgctg
     2941 taggggatgg caaacaacga attatgttat gggctggtgc tagaaagtca atatttgaag
     3001 attttcaaaa tgatttttta agtcaaaaaa gaacattaat tatgaatcat cgttcagcgc
     3061 caagacttgt agagattcaa aaagcaatgt ataacagttt gaatgaagag gcaattgatt
     3121 taaaaactaa tgaaaagtgg aattatgaag atggagaagt gtatttaaga ctttttgagg
     3181 attatataaa agaagctgat atacttagtg aggaaatagt cgaattggtg gaaacaggta
     3241 tagaaattaa tgaaatatgt atattagtaa aacagagcgt agatatttat tcaaaagaga
     3301 ttataaataa actttctgaa cgtaatatta aggcgcgtaa tgaagcaatg tatcaggaga
     3361 tattaaaaga agatataata aagacactgg tttcgctttt taggttgtcg ttaagtgata
     3421 gaaatcccga tgattgggat tatatttacg atattctagg tgaattatat ggaatagacg
     3481 aagggtataa ccctaaaata ttaaatgatt tcattaccaa agtagaatta atgattaatg
     3541 atctcaaggt aaatttaaat attactgatg aaaaacaatt ttcagattta gttgattcta
     3601 aggttgaata tatttcatat gaaaaatttg catcaaagtt tcaaaaatat aaatctaaag
     3661 attattttga tgatttggta gataaattta aaaagctggt atggattgaa tataatgaaa
     3721 ctaaagattg gattaaagcg attgataact ttgaaggtaa aaattcaatt ccaataatga
     3781 caattcataa aagtaaagga cttgaatatg atactatctt ttttgttggg ttagaagatt
     3841 cagcattttg gaattttaaa aatcaaccag atgaagatag atgtgcattt tttgttgcat
     3901 tatcaagagc taaaagaaga attgatttta catttagctt agatagacca gcaggatttt
     3961 cgaattcaca gaagcattat tgtattaatg aattctacga attgtttgat aatactgaga
     4021 ttgtaaaaaa gatagagtat tgattaagat gataaaaact ttagtatatt atattttaag
     4081 atattagtta tataagtaga cattgaattt atgtatttga tgtctatttt ttatataaaa
     4141 atatattaaa gaaaggatga atttgcatga agaaaaaaac aaaaatatct aaaaagattg
     4201 taagttttat tatggttttt ataactatag ttactactat acttacatca tcagtttcag
     4261 cttatgctag cgagttggat atgagtgaac agacaggctt taattattta ggtattagtc
     4321 caattacagg aaaaaaatta atcataacat ttatcgtatg aagatggata ataaaattgt
     4381 attttgtgta gagccaggaa ttaaaacttc aagtggagga ggatatatat cggaggagtt
     4441 aggagataaa tttatatcta caacacttcc aaattatcat aaaagaaaag ctgaaatact
     4501 tgagaaggta aatagacata atatattacc ttcatggaat aatgaaaaag ttgcagttaa
     4561 aagtggagaa acaattgagt tagaagataa gaatggtatt attagagaaa tgcaattaga
     4621 atcaaataat actggaacag atatattact tgaagaaaat actttgaaaa ttacagcaaa
     4681 taaaaattct aattcaggag ttattagtta tataaagatt cctaaaaatg aggttggaat
     4741 atcaatagtt tatcgtaagc ataactatca aagcttatca gaatttcata tggaaagttc
     4801 gatgagtgca aatttaaata taaatgtaat caagttagga attgttaaag taaaaaagat
     4861 tgatgaaggc acaggaaagg aattagcaaa tgcaaaactt agatttgagt atgatggtga
     4921 tagtaaagaa attataacgg gttctaatga aattgctagt atagaagata ttattggttg
     4981 attaaaaagt gaagtatact tttgtatcta tgaaaaagac tataagcaat atataaaatt
     5041 agatatacct attgatgaag caaaaataaa aaatagattt gaaataaggt taaaaaatga
     5101 aagagcatat catgcagcag ttgatttgtt agcaaataga gatgaagaaa agacagcatt
     5161 ttcaattatt aatcgttata tacgttttgt tgataaagat gatgaaaaaa ttagaagtga
     5221 atggaaaact aatgatcagt ggagttattt tattggtgaa ggtagagcga agttaaaact
     5281 aacaacaaat ccagaaccat ataatattca aaaaacttta aattggttat caagacaagt
     5341 tgcaccaacg ttaaaaacaa ttaaaaggtt agatagtttg aacaactcaa cattaattga
     5401 gggcttaata aggtatgcaa gactaacaga taaacataaa aaaataattg aacaaaattc
     5461 agtaaatgtt gaagacatta tttatgatga ataaaaaaat gtaattagta ttgcagattt
     5521 taatataaaa gaagggagat atacgaatga attttggtca aaatttatat aattagtttt
     5581 tatcaaatgc acaatcattg gtattaatgg ctatagctat aattggtgta tatttaggat
     5641 ttaagagaga gttttcaaaa cttataggat ttttagtagt tgcattaatt gcagtagggc
     5701 ttgtgtttaa tacagcagga gttaaagata tattattgga gctatttaat aaaataatag
     5761 gtgtgtaagg attaaaagag aatagaaaat tatgagtata tgatgaaggg ttctaataag
     5821 ttttattaaa atatatattg aaagacaagt agagctattt cgagttatta catgtaatgc
     5881 taatatatat aactgcattt tggaaattaa atacataatt agaaatagca aattttatag
     5941 gtgacttatt aatatgttta cttattgtat actatttgat atatttaata tgaggtgatt
     6001 cttgaaaatg gacatagtga aagtttttgc taacaatgtg agaaaatata gaaataaaaa
     6061 aggtctatca caagaagctt ttgctgaaaa agctggattg catagaactt atatttgtgc
     6121 tattgaaaga aaaaagagaa gtattgcttt agataatgtg cagaaaattg ctgatgcgct
     6181 tgagattgag acttacttac tatttataga aggtgaggag aaagaatgtc tatgaaaaaa
     6241 ttaccaatag aaaagcttat agaaaattta ggtttttcag ggtcaaagaa tcttgtgaat
     6301 atagatatgc ttacaaaatt agatatatct aaacatgatg taaaaacttt aaaagttata
     6361 aatccttatg caatttattt agtaaatagt aagccattta tattatttat agagtcttct
     6421 tttgaagaaa aaaaattaca acaaattagt aagcttatat ggaatgctca aataccaatt
     6481 gcttttatat gcgattctaa ttctgttaaa atatttaatg gaaaagcttt ggatttaaag
     6541 tcatatttaa ttgaggaaat atataatcaa tctattgagg atttttcgga agaatctgag
     6601 ttttcttatt tgaaaatatc tgatccatta ttttgggatc aatacactaa acaatatacc
     6661 aaaacgcatt taaatgatta tttgctagat aatattactt cattgacaaa taaattaaag
     6721 aaaaaatata aggtgaaatt tgcaacgaag cttgttctaa gattaatatt tattagatat
     6781 ttaatcgata gaggtgtcga tatagcttat aaaaatttta atcatgatat taaagcttca
     6841 caaaaagaac tgctaaagat tactaaaaac aaagttgaat tatatgaact gtttaaatac
     6901 ttaaaagaaa agtttaatgg taatttattt gagttaaatg acgaaattaa taatacttgt
     6961 ttaacagatg aagtgtttat tttactatct gattttttat caggtaaaat aagtattgac
     7021 aatggacaaa tgtctttatt ttcaatgtat gactttaata tcataccagt tgaactaatt
     7081 agtaatatat acgaaattct tttaggaaaa gaaattcgta ataaagataa cgcattctat
     7141 actccgaatt acttagcaga atatatttta gataaaacaa cacttgaatt tctgaaaaat
     7201 aataggcaat acaaaattct agatccttct tgtggatcag gagtattttt agtaaatagt
     7261 tttagacgta ttattgatat gaatttgggt gataaacttt tttgtgaaga tgataaactt
     7321 ttaaaagaac ttttattatc taatatttat ggaattgata tcaatgaaga tgctatcgat
     7381 gttactattt tctcgttata tctaactatt ttagattaca aagaccctaa gatattatca
     7441 gaatttaaac tgcctaattt aaaagggtgt aatttaatag cgagtgattt ttttgatgat
     7501 aataaattgg agagtttaaa gaaaattaac tttgatttta tcattggaaa tccgccttgg
     7561 ggaagtgtct cagatggatt acatttagat tattgcaata aaaatggcta taatgatatg
     7621 caacaaaaca aagaaatcag tagaagtttt atttttagag caaaagattt tagctctaga
     7681 aatacagtat gttcttttgt agtgcattct aaaatactat atactcaaaa aaagccatca
     7741 aaaaaattcc gtgaattttt gcttaaaaaa agtcaaatat ttagtgtagt tgaaatgtca
     7801 tctgtaagaa aattagtgtt taaaaatgca gacgctcctg cagcagttat atcatttaag
     7861 tataacgaaa gtgaagaaaa tttaaataat agaatcatct atacatcttt aaaaccaaat
     7921 gtatttttta aattatttaa cattattgta attgaaaaat atgatgttaa atatatttca
     7981 caaaaactat tattggagaa tgattgggct tggaaaacta ttgtgtatgg attcagtaaa
     8041 gatatagata taattatgaa tatcaaaaaa aaatatcaaa ctgtaaatga aattattttg
     8101 aaaaacagtt ttgttcatgg aacagggata caaatcaatg gtaatgaaag ccatgatgcg
     8161 acacatttaa aaggaaagtg gtatgttgat tcaaagaaag gaatagattc attctatgtt
     8221 gatataaata aagggaaaga acttactcaa gataaaatga gaagacctaa ggctaatcag
     8281 caagaaatat ttattcctcc atatgtatta ttaaaaaagg gagttgacac gaaaacatat
     8341 agatatagag cagcattttc aaatgatgca tttgtatatt cagatgcagt tacaggaata
     8401 tgtggaaaaa aagataattt aactacactt tattgcttga ctggtatgct taactcttct
     8461 ttttactcat acttaaatat aatgctaggt acatcaattg gaatagaaag agaacaagga
     8521 ttttcaacgg aaatttttac gtatccagca atagaaagtg atgaaattgc taatctggta
     8581 gtaaaaatac aagaagcgac taaattagaa aaaacaacta tgttcttgaa tgaaaaaaat
     8641 agttgtaagt atatcaaaca attagataag ttaattttga agtcttttaa tttaaatgat
     8701 gataaattta ttgactatgc aataaatatt caaattcctg agcttacaaa ttctaagtca
     8761 actaacatat atagaaaaat aactaaggaa gatttaatta attatagtga atgtttcaaa
     8821 gaacagtttt catcgatata tcgtagggtt gggaaaagta tttcaatttc ggtatatcca
     8881 aaaatattaa atcgttttac agtatttgaa ctagaagtaa cagatgaaaa aacagataga
     8941 gagattatgg ttaaagcaga agctgataat aatatggagt tgtttacgaa aataattaga
     9001 tatgattata atgatgtttt tcatcaaatt agagatatta ttcattttgg agaaaattca
     9061 ttttatatca taaagcctaa ttgctataaa tattggcatc ctgctatcgc agaaatggat
     9121 cttgctgatg ttttggatca gattttatct gatacagggg gtgaagaata atgggaggat
     9181 ttaaaaattc ttcactaaac ttttatttaa agcaagggct ttatgaaata attatagatg
     9241 gattaataga ctgttgttta cttatgagca aatcatgtat tgaacaatct agaaaaatat
     9301 taaatcacga agaaaagata agaacgcatt tagttgaaaa ttatttagaa aatgatattg
     9361 aaagaagaaa aacatcttta tctaaacaac atctaagatt tgaagtagaa gcacaagaaa
     9421 attatgacaa caaaaatgaa tcttatgagg gtagggttga tataaaagtt atatctaaag
     9481 acactttagc ggatagaagg gcttattata tcattgaatg taaaaggata gatggtaagg
     9541 aaactttaaa taaaaaatat ataaccgaag gtgtacaacg atttattact gagaaatatt
     9601 cttcgcatta tggcaaaaat ataatgcttg gatttatagt aaaagatata aatatccaga
     9661 gcaatataca aaaaattaat tcactgaatc agaacatatt atatacatat atagatacag
     9721 atatgttgac agttagaagt gattctgaaa ataattatgc tgtttgtgaa agtaaatata
     9781 aagtaaaatc taaactacta ttgttaaatc atatttttta taacttttcg agtataataa
     9841 gataatctta tgaatataaa atattactat gttaaaatta tagtttggtt ttgataatat
     9901 tataaaaata agtttaagaa taaaatataa aggaaaaaag gggcgtagca ctatttttta
     9961 gtgtgacagc ccctttaatt ttaaaatcgg taaggaggag tgtatttgaa aaaaattaag
    10021 tcatatacag ggattttgaa tgtggagaag gttatatatg caataaatga ttttcagctt
    10081 ccatttccag ttacttttac gcagatgaca tggtttgtat taacactttt tataattata
    10141 ttatttggta agttacctcc attatttatg atagaaggag catttttaaa gtatcttggc
    10201 ataccaattt ttataactta gtttatgtct caaaagactt ttgatggaaa gaaaacatat
    10261 ggatttttaa agtcaataat actatattca ttaagaccta aaataacata cgcaggaaaa
    10321 aacattaaat ttaaaaataa aattattgat gaaaatataa ctatagtaag gagtgaatta
    10381 tatgttcccg ataaaatata tagagaacaa tcttgtattt aataaggaag gagaatgttt
    10441 tgcatattat gaattaatac catataatta ttcattctta taagctgaac aaaaatttat
    10501 tgtacatgat aattttaggc aattaatatc acaaagtaga aatggtaaaa ttcatgcact
    10561 tcaaatagcc actgaaagtt ctataagagt tacacaagaa aggttaaaga aaaatattac
    10621 aggaaaatta aaagaaattg cttatgaaaa aatagacgag caaacagagg cacttgtttc
    10681 tatgattggc gataatcaaa ttgattatcg tttttttatt gggtttaagc ttttagttac
    10741 agagaaagaa atgaatataa agagtataaa aaagtcaata ttagctaatc tatcagattt
    10801 tatatatgat gttaataata aattgatgga tgactttgtc agtataaata atgatgaaat
    10861 tatgagattt ttaaaaatgg aaaagttatt agaaaataaa atctcaagaa gatttaaagt
    10921 tagaaggtta gataaaaatg attttggata tttaattgaa cataattatg gaaagaaagg
    10981 cacagttttt gaagattatc aataccattt acctacaaag aaacttaaaa gagaaacatt
    11041 agtaaagaaa tacaatctta taaagccaac tagatgtctt atagaagaga agcaaagata
    11101 cttaaaaatt atacatgagg attcagaaag ttttgtagct tattttacta ttgaaattat
    11161 tattggagaa atgaattttc catctagtga gatattctat tatcagcaac aacagtttgc
    11221 atttcctatt gatactagta tgaatgtgga aattgtaaca aataaaaagg ctttaactac
    11281 agttagaaat aagaaaaaag aattaaaaga tttagacaat catgcttggc agtcagggaa
    11341 tgaaacagta acaaatgtag tagaagcatt agaggctgta aatgagcttg taaataattt
    11401 aaatcaaaca aaagaatcaa tgtataagtt aagctatgta attagggtat cagcagatag
    11461 cattgatgaa ttaaaacgcc gttgtgatga agttaaagat ttttatgacg atttaaatat
    11521 aaaattagta cgcctaatgg gagatatgat aggatttcac aatgaattta taccttcaag
    11581 caaaaggtat ataaatgatt atattcaata tgtaactagt gattttctag caggattagg
    11641 gtttggagca actcaatagc ttggagagaa cgaaggaata tatattggat atagtttaga
    11701 tacaggaaga aatgtctatc ttcaaccatc tcttgcaagt caaggagtaa aagaatctgt
    11761 aactaatgcg ttggtagcag cattctttgg atttttagga ggtgggaaat catttgctaa
    11821 taacttactt gtttactatt cagtattgtt tggagggcaa gctgttttgg tggatccaaa
    11881 agctgaacgt ggcaaatgga aagaaaattt accagatata tatcatgaag taaatattgt
    11941 taatttaaca agtgatatag aaaataaggg aatgctagat ccttatgtaa ttatgaaaga
    12001 agtaaaagat agtggaagtc tagctattga tatattaact tttcttacag gaataagtag
    12061 tcatgatgga gagcagtttc ctatacttag aaaatcaatc ataaatgtta ctcaagacaa
    12121 atgcagagga cttttgaagg taatagatga attaaggaaa gataattctc aaatatcaaa
    12181 taatattgca gatcatattg aaagttttac agattatgat tttgcacaat tacttttctc
    12241 agatggaaat attaaaggct caattaatct agaaaaacaa atgaatataa tacaagttgc
    12301 agatcttgtt ttaccgaata aagatacttt atttgaagaa tatacaacaa tggaaagtct
    12361 ttcagtagct atgttgatag taattagtac ttttgcatta gattttatcc atacagatag
    12421 aagtactttt aaaatagtag atttagatga agcttggaca tttcttaatg tagcacaagg
    12481 aaaaacttta tctaataaat tagttagagc aggatgtgct atgaatgcag gagtgtattt
    12541 tgtaactcaa aatgcaggtg atgtatcaga tgaaaattta aaaaacaata ttggattaaa
    12601 atttgcattc agaagcacag atatcaatga aattaagaga atacttgaat tttttggagt
    12661 tgataaggaa gatgagaata atcaaaaaag gttaagagag ttagagaatg gacagtgttt
    12721 aatacaagat ttatatggac gagttggggt aatacaaata catctagttt ttcaagaact
    12781 ccttactgca tttgatacaa gaccacctat aagaaaagag gtggattaat gaaatcgaaa
    12841 gtatttagaa atttaagctg gataactatt ttaatagtag tacttttact attttttatt
    12901 tatataaatt tatagattta gagaaacaaa tagatacatt agataagaaa agaaaaaaac
    12961 tcatagatat gagattagaa gatataattg ataaagaatc atatgaagaa aagtatgatg
    13021 atttaacagt tgaaataaag aaacttagta aagagaaaaa aactcttaaa gcatcattaa
    13081 taaatgaaaa gagtataaaa gcaagactta gagaatttag aaagatactt gaaagaaatg
    13141 aattgttaga aaaatttgat agatatgtat ttgcaagtat agtagataag gttatagttg
    13201 gagttattaa tgaagatggt atagttgatc cttataaaat aacttttgtt tataaaacta
    13261 gctttgatga taaacaagat ggacataaat ttataaataa gagtccgtta gaaacgtatt
    13321 caaaagataa caacgataat aaaaagaagt gttcatatac taataatgag acattgcgag
    13381 actgtagtta aacttattag agaataacaa ggtttcagac ttttatattg tgtcttaaaa
    13441 gtagatttgc cacctatttg ccaccataat tatttttggg tggcaaattt attctacaat
    13501 gatttcaaaa atatttaccg tctggtttct catgttgtta gttacatgta aatatgtatc
    13561 catagttgtt gaagttttag catgtccgag tatatgctgg atatctttta tattagtacc
    13621 attttctaat aacatagtat cagggttttt aaaataaagt gtgtattctt ctaaagtagt
    13681 gataattaaa atatcaaata tcactataca aaggaaagtg cacgcttttt atgtttaaca
    13741 aaaacgaaat attaaaaaac ttattgaaga atatgatgtc aaaacaacta cagatattca
    13801 agatatgcta aaagatttat ttacaaatac aattcatcaa atatttacat tgtcgaattt
    13861 tagcggttta aacataatat attgactgat ataataaaat catagaatat tttaggggga
    13921 aatagattat gtttaagaaa aagttaatag ctgttgcaat gagtataact atgattagtg
    13981 tagggagctt ttcgtatgct cattcaggaa gaactgattc atcaggaggt catcgtgata
    14041 ataaaaataa atctggatta ggaagttatc actatcattg tggtggttat ccagctcatt
    14101 tacataataa tggtggttgt ccttatactg gaggaggaag cagttctgga acaacaactt
    14161 cggttaataa tgaagaaaag caaaagaggt ctgttggaga aaaaggatat aatcaagggt
    14221 atgaagatgg gtataaagga aattatagtt catcaaatta tagtggagat tatagtgata
    14281 catatgaatc aaagtactca gaaggatatg aaaaaggtaa ggctaaatta gaagaggaag
    14341 aaaaggtggc taaagagact ggatataatt tagggcttac aggtgctaaa tctaataata
    14401 catatgaaaa agaagtttta aaaaatgctt atgataccgg atattcaact ggatacaatg
    14461 aatataagac taaaaagata gaagaatata aagctaaagg gatagaggat agcaataaag
    14521 ataaggaaaa aatgacattt gaagaaaaca tagattctga atttatagat gcttataata
    14581 atgcttatga cgaaatacaa gaacaattaa aaaatgatta cactactcaa ggatttgaga
    14641 gtgctatcaa aggagaaaga tttgacacat caactatagg taatgttaaa tatgctaatt
    14701 ggtttaaaga aggatatgat aatggtaagg ttaagttacc taaagcaaaa gaaagtgtat
    14761 ataatcaagg ttacaatgaa gaagatttta gtataccaga tgaaatgaag tctattgaaa
    14821 ctaagttaaa aggtgtatat gatgaaggtt tagaaaaaag agaagaagag aaaagtagaa
    14881 atgctactta tggggttgga gctggaacag cagctgtagt tgctggagta gtatataaga
    14941 agaaaaaacg taaaaagata tagatgctag atataccaat gatgacaagt gtataacaaa
    15001 tataatttta agtataaata tttaaaatat agaagggaag gttatatcta tgggatttaa
    15061 ctttaggaaa agcataaatc taggaggagg actcaaatta aatttaggta agaagagtgt
    15121 aggtataagt gctggtgtta aaggtgctag agtgtcagtt aattcaaaag gaagaaaatc
    15181 agctacattg agtatacctg gaactggaat atcatataca aaaactagta caaagaaacg
    15241 taaacctaaa taaatatttg atatactaag aaatagactg ttacaaatag tctatttttt
    15301 agtatataga tttaaaaagt gacaaaattt tgaaatataa actaatttca taaaggaaaa
    15361 tattatttta tgtagaatta taatatttaa gtaatattat aaaatttata ggaggatttc
    15421 attatgaaat taagcaaaat actattggca atagggatgt ctgtttcatt attgattgct
    15481 agttgtccaa catcaaacgc tcttagttct attaattcaa taaaaggtac aaataagtat
    15541 gaaacagcag gtttaatagc agataaacaa aattatgata ttgcaatact tattaattct
    15601 gaaaatggat tatctgatgg attgtcagca agtggattgt caggagctaa aaatgcccca
    15661 atattgttaa ctaagaaaga tacaatccct tcagaaacat taaaaagatt agaaaaggta
    15721 aaagaagtat atctaattgg aggaaaaaat tcgataggag catcagttca gaaaatatta
    15781 agtgataaaa aaataagcat aaatagaata agtggaactg atagaataga tacaagtcat
    15841 aaagttgcaa aagaaatagg aagagtaagt acggttaaaa aagtaatatt aactaatgct
    15901 tttaaaggcg aaccagatgc tatgagtgtt gcttctatag cagttagaga taaatctcca
    15961 atagtattaa ctgatggaaa tactacaagt tttgctacag atggattaga aacttatgca
    16021 attggtggaa aagtcagtat gagtgataag ttaataaatg aaactaaagc aacaagaata
    16081 ggtggagtag atagatacga tacaaataaa caaataataa ataaatttta taatggctca
    16141 aaagaatttt acatagcaag tgggactgat ttagtttatt ctttagttgg ttctacaatt
    16201 gcacaaaaaa cacctatagt gttagttgga aatggaagta ataagagtat attaaaaaat
    16261 gcaagtaaaa taactgctat tggaaatatt gatgatgttg ttataaaaca atgtttaaat
    16321 ataactaata atgttgggga ttcaaataca ggtattgtaa aagatactaa ttcaaataat
    16381 acgaacaaac caagtaatga gcctagctct aataatcctg gattaactga taaaagtaca
    16441 gtatatgtaa ctcctaatgg aaaatcatat catctaacaa aaaattgttc tacactaaaa
    16501 agaagtaaaa caataaataa agtaacatta aaacaagcta agtcacaagg taaaatagat
    16561 ccttgtaatg tttgtgtaaa ataatgtatt agccaaatat atattaaaaa agacaattaa
    16621 ttaagattgt cttt
//
LOCUS       DAMJZE010000086        16634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJZE010000086 DAMJZE010000000
VERSION     DAMJZE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.087"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            8..1132
                     /locus_tag="PC541_002285"
     CDS             8..1132
                     /locus_tag="PC541_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2839745.1"
                     /translation="MTFLFVKYIIKQSTVLSIVSTLLVYLIEQLVYGFTAPLNYLIFT
                     SDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLII
                     LFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQ
                     ALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKA
                     QDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDF
                     DLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGI
                     GLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1171..1656
                     /locus_tag="PC541_002286"
     CDS             1171..1656
                     /locus_tag="PC541_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839746.1"
                     /translation="MYLKRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(2311..2535)
                     /locus_tag="PC541_002287"
     CDS             complement(2311..2535)
                     /locus_tag="PC541_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2839747.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2632..3885)
                     /locus_tag="PC541_002288"
     CDS             complement(2632..3885)
                     /locus_tag="PC541_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2839748.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4145..5134
                     /locus_tag="PC541_002289"
     CDS             4145..5134
                     /locus_tag="PC541_002289"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2839749.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5206..5946
                     /locus_tag="PC541_002290"
     CDS             5206..5946
                     /locus_tag="PC541_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2839750.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6146..7297
                     /locus_tag="PC541_002291"
     CDS             6146..7297
                     /locus_tag="PC541_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2839751.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7322..8077
                     /locus_tag="PC541_002292"
     CDS             7322..8077
                     /locus_tag="PC541_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2839752.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8269..10656
                     /locus_tag="PC541_002293"
     CDS             8269..10656
                     /locus_tag="PC541_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2839753.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10887..11120)
                     /locus_tag="PC541_002294"
     CDS             complement(10887..11120)
                     /locus_tag="PC541_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2839754.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11409..11999
                     /locus_tag="PC541_002295"
     CDS             11409..11999
                     /locus_tag="PC541_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2839755.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            12113..12958
                     /locus_tag="PC541_002296"
     CDS             12113..12958
                     /locus_tag="PC541_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839756.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12955..13638
                     /locus_tag="PC541_002297"
     CDS             12955..13638
                     /locus_tag="PC541_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839757.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13635..14351
                     /locus_tag="PC541_002298"
     CDS             13635..14351
                     /locus_tag="PC541_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839758.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            14448..14711
                     /locus_tag="PC541_002299"
     CDS             14448..14711
                     /locus_tag="PC541_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839759.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14683..15138
                     /gene="def"
                     /locus_tag="PC541_002300"
     CDS             14683..15138
                     /gene="def"
                     /locus_tag="PC541_002300"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2839760.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            15190..15651
                     /locus_tag="PC541_002301"
     CDS             15190..15651
                     /locus_tag="PC541_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2839761.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            15701..16603
                     /locus_tag="PC541_002302"
     CDS             15701..16603
                     /locus_tag="PC541_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2839762.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
BASE COUNT         6626 a         1762 c         2686 g         5560 t
ORIGIN      
        1 ttttcttatg acttttttat ttgtaaagta cataataaaa caatctactg ttttatctat
       61 agtatcaact ttgttagtat atttaattga acaattagta tatggattta cagctccatt
      121 aaattacttg atatttacta gtgatataaa tacagatatt tataaatttg atataaatat
      181 gggactatct tctttaatta ctattataat agctggattt atatattggt atagttctaa
      241 aaagtttaat attaaggtca tgaactttga taaatatata gcaattttga tgattccatt
      301 gttgcttata atattattta tgcaatcatt tgaatatagt tcaaatataa atatagatac
      361 aagtcttggg attgtaaagc tgctattaaa tacttctata actgaagaaa ttcaagcttt
      421 tcttttttct ataattggaa ctatatgtgt atttttttct ctatttacct ttaagaaact
      481 aattcaagct ctcgaagatg acaaggagag ggcaattatg aatcaacaga ttcatgctca
      541 aaaaaactat attgaagaag cgaaatcacg attatcacaa actatatcct ttagacatga
      601 ttttaataat catttagcta ttgtcaatgg attgttgaaa aaagaccaaa ttttaaaagc
      661 acaagattat ttaaataagc ttgaaaaagt aacaagtgac ctatcatttt cttgtaatac
      721 acagaatatt gttattgatg ctcttttcaa caataaatta agtatagcaa aacaattagg
      781 catagaggta gattgtgaag taattatacc ccaaagcact aacatgaacg attttgacct
      841 atgtatagtg tttgctaatg ctattgacaa tgcaattaaa gcttgtagag ttgtagataa
      901 acaaaatagg tatttaaagc tttccactaa gttagagggt ggatttttta tgatagaaat
      961 aaaaaatagc tataatccaa taaataatta tacaagtggc tcaggtatag gtcttttaaa
     1021 tataaaagaa attgctaaaa aatatcaagg ggctataagt atagataaga catcagacta
     1081 ttttcaaata aatgtattgc tagttatttc attacattgc gctagtattt gagaacaatt
     1141 tcattgaaaa tagtatttgt aagctatatt atgtatttga aaagagaaag tttgatatat
     1201 agaaatgagg aggttactaa aatgaaagtt ggaaatataa atggtcaagc accaattaca
     1261 aatggtttag atagttgtca aagtgaggta caaggcttag aacaaaagaa aaaagcatta
     1321 caggatgaaa tagagaaagt aagaagtgat aaaagtattg atggtgaaac ggccaaatta
     1381 aaaatagaag ctttaaaaaa agaaatggaa gagattgaaa ataaaattca agagttaaaa
     1441 ggaaataaag gaaaaaaaac aaataaagaa gaaaataacg tttcagaagt aaataataat
     1501 ctattagaga gaaaaaaaga accttctaat aatgaaaatg taaaacaatt atatgatgtt
     1561 tttgaaagaa gcaatgtaga cgaacaatgg gataatactt atcgtgttct ccaagatgat
     1621 aaaaacttaa aagtaaaatt taatagacct atatagttta tttataaaaa tagttaatat
     1681 tttaattatc accacttttt agaagaatac acactttatt ttaaagaccc acatatttat
     1741 ttacagttag cacatttata tgtggtttta taactattat ttacaagtct atattgataa
     1801 cttttacata gtatatgatg tttaaatact tattcaaatt tatctataca taaacaaaaa
     1861 aagagatttt aaaatctctt tttagattgt tgacttatgg ctttttgatt taataaaatc
     1921 aagaagccgt ttgaatttta tatgttggct gatacccttc tgatattgaa acaccatttt
     1981 tgtttccaaa atcgtttaaa attaagaact tattatgaat gactatttca ttatgaacaa
     2041 taagcaaata acatgaatct tcaatcaatg ttttaacagt agcattaaat gcttgcttat
     2101 atagttctat cgtttgtttg aactgcccat taaagtaaat atgagtcaat aacataacta
     2161 tatctacaca aattaatata aattttttat actgtacatt atctaactgt tgattatctg
     2221 tattacaaca tgattgaatt taaaaagcca tcaacacata gaatgttgat ggcctgaaaa
     2281 tagattttaa aatctctttt ttattatata ttaattattt tttaaattta tattttttgc
     2341 atcagatttt ctaatagcta agtcaaaccc tctaaatcta atcactatag ggtcattaag
     2401 aggagctatc tttttcactt caacttcagt gccatctatt agtcctaagg ctagaagtct
     2461 cttcataagt ttttcatttc cagctatatt gtctataatt cccttttgtc caagttttaa
     2521 gttgtaaaca gtcattatta acacctccta atgaaaatca ttatcaaact ttatatttat
     2581 aatatataac aaaaagagca aattgtcaat atataacaaa aaataaacat actaagttag
     2641 tatgtttata aaaaatagta tacatgggat actaatagca tctagcaaaa aaccacttac
     2701 taatggtaca actaaaaagg cttttggaga atcaccatat ttttgtgtca aagaactcat
     2761 atttgccaga gcgttcggag tagctcctat tccatgtcca atcaaaccac ttattatgat
     2821 ggcagaatca aaatcttttc caagtactct aaagcaaata aatattgaaa ataggattat
     2881 aaagattact tgaaaagtta ctattaagag aataggaggt aataatgtag ataattcaaa
     2941 taaatctata ttcataagtg ataaagttaa aaaaattccc aatgaaagtt cttttaaaaa
     3001 atctataagt ttaaaattta attctagtat atttatctta tcatttaaat ttctaaaaat
     3061 aacagccgaa aacatacaag ttacaacaga gggtaatagt atattaaacg ttatatagaa
     3121 gcatctactt ataatttctc ctgtatttat acaaataaat atgagaagaa cctgttccaa
     3181 aaatacagat ggtgttattg tttcggagaa gttattattt tttatcctat tcttatttct
     3241 aaaaaattta cttccaaatc tctttagatt tctattatat ctaggaatcg acaagtctat
     3301 atttgatgat ggttttaatt tatatttatt tattagaaat ttagctacag gacatcccaa
     3361 tataccaccc ataataagtc ctattgttgc agcagataat ccaaccccaa ctgctccttc
     3421 tataccaaga ttttctattg taagtccaaa tgcagcagaa taaccatgac caccttccat
     3481 agatacattt ccacacataa gccctaaaag aggttcaagt tttataatct ttgatattgt
     3541 aatagctaat atattttgac aaaatgccaa tactccacac aaaatccaat atctaaataa
     3601 tacactacca cctttcttta taagatttat gcttatttca agtcctatag ttgtaaaaaa
     3661 gaaggttatg aaatttggca ttaaatttcc acttatagat atatctgcta tattaaaata
     3721 tttaaaaaat aaatttagaa aacaaaataa tagacctcct attactggtg caggtataca
     3781 gaaattattt aaaaagttaa ctttattctt caataaatta ccaattaaaa agaagaatat
     3841 agatagtgtc aaggtttgca ctatatcaaa acttaaggta atcaaaataa gtcctccttt
     3901 gtaattcaaa gttttaatta tataacttaa attcttatgt attgaaattt tattatcaaa
     3961 tgtatattaa taaatgtata ttaaaaagaa tcagtagata taattagata gtcataaatt
     4021 aaattaaaat attttaatga gatatgactt ttttagaatt aaaaaccgta gtatattgat
     4081 ataatagaaa taagacttaa aaatctgttg atagtattat ttataagtac agtaggagga
     4141 taaaatgttt gaaaatttac ctaagataga tttacattgc cacttagatg gaagtgttag
     4201 agtagaaacc atgcttgata tagctattaa agagaaaata gatttacctt caaataatat
     4261 ggatgaaata aaaaaattag caaaggtttc ttttaattgt acttcactag atgaatattt
     4321 agaaaaattt gatttaccct taaaagtaat gcaatctaag gaaaatttaa aaagaattac
     4381 ttttgaactt ttggaagatg caagtagaga aaatgtaaaa tatattgaaa ttagatttgc
     4441 accattactt cacacccaaa aaggtatgag tgttaaaaat ataattgagg gtataattga
     4501 aggaattaga gaagctgaaa gtatttatga tataaaaggc aatttaatat taggttgtat
     4561 gagaacaatg acctcaaaag aggcattatt agtaattgaa gaagggaaat catttgtaaa
     4621 taaaggtgtt gtagcagtag acttgtgtgg tccagagaaa gagggatttt gtaaggaata
     4681 taaagatgtt tttaaattag caagagaata tggatataaa gttacaatac atgcaggaga
     4741 agctgcaagt ggagaaaatg tattggatgc tataaatatt ttaaaggcgg ataggattgg
     4801 tcatggggta aaaattaaag accataaaaa ggcgtataat ttagttaaag ataaaaaaat
     4861 attgcttgaa ttatgtccaa ctagtaatgt acaaactaag acagtagatt cttatgaagc
     4921 acatccattt tatacttttt ataaagataa tttacatgta agtataaata ctgataatag
     4981 aactgtatca gatataaatt taagttcaga actaaatgtt atatttgata cttttaaact
     5041 aggattagaa gactataaaa taatttatag aaatgcagta gaagctagtt ttgcagataa
     5101 agaaactaaa gagtatttga atagtttaat ttaatagata aaaatattta aactttaaat
     5161 taagctatta aattgataaa aatacattgt attaggagaa aaaatgtgaa agattatgaa
     5221 gatgttgttt atattaaact agtcaaaaaa gaacttgaag aatggaagaa aaatatatta
     5281 aagaaacctt ctatatttga caaagcatca aagggtatac agaataaatt taatggagta
     5341 ttgccagata aatatcatga aataattact tcttctataa aagtaatgac aaaaaccgta
     5401 ctgtttggat ataaatatgc tacaaagcca tcacttagaa atatgtcatt agaagaaaga
     5461 gaaaaattag ttttagaaaa agctaaaact tacaagacta cagcaatgat tgaaggggca
     5521 ggaacagggg caggaggaat cctcttaggt ttatctgatt ttcctttgct tttaggtatt
     5581 aaagtgaaat ttttatatga tgtagcttca atatatggat atgatgttaa agattataga
     5641 gaaaggattt atatattaaa tatattacag ttggcttttt ctagtcaaca gcatataaac
     5701 aaaactttta aattgatgaa taactgggat gaatatgtaa atagtctacc agaagacatc
     5761 aatgattttg attggagaac atttcaacaa gagtataggg attatttaga tttagctaag
     5821 tttttacaac taatgcctgg aataggagca tttgtaggat tttatgtgaa tggaaaatta
     5881 attgataaat tatgtgaaac tgctgtgtct gcttatagat tgaggattcg agagcttaag
     5941 ttataagaga aattataaca taaaaaatta gttatacaat atatctatat gtatctaatt
     6001 ttgcattgat aaaaacaatt atggataaaa ggtaaagcat gatagaatct aatgtagagg
     6061 tttgtatttt aatggaaaaa tttagttttt attgcttaag atacaaattg aattacgaag
     6121 agaagtaatt gggaggaata ttttcatgac aaatttgcca aaacaatttg atagcttcaa
     6181 tgaagctaga caaaaaggat ttataaatgc aaaggaactt aaggaaagtg ggaaaaagat
     6241 ggttggagtt ttctgtacat ttactccagt tgaaattcca atggcagctg gagcaactgt
     6301 agtaggtgta tgcggcgtta gtgaagagcc aatacctgat gcagaaaggg tgcttccaag
     6361 aaatctttgt cctttaatta aatcaagcta tggacatgca ataacagata cctgtcctta
     6421 tttctatttt tctgatttgt tgataggaga aaccacttgt gatggtaaga aaaaaatgta
     6481 tgaagaactt gcaaaagtta agccaacata tgtaatgcat ttgccaaata cttcaaaagg
     6541 agaatttgcc tataaacttt ggaaagatga aatgataaga ttaaaagaag aggttgagaa
     6601 atctttaggt gtaactataa ctgaagaaga tataagaact gctataaaag ataaaaatga
     6661 agaaagagaa cttttaaaag agttttatgc acttggtaaa ttacaaccat ctgcacttac
     6721 aggccttgag ttacataatg ttttatatca agcaggtttt aaatttgacc gtgcagaact
     6781 taaacattct ttaagaaaag ttatagatga tatgaaagaa agatatgaaa aaggtgaatg
     6841 tcctgttcaa aaagataagc caagaatatt gataacaggt tctcctattg gtggaataag
     6901 cgaaaaaata gttaaaactt tagaagatgc aggggcatca gtcgttgcat atgaactttg
     6961 tggagctatt agatcaaacg atttattggt tgatgaagaa atagaagatg tatatgatgc
     7021 tttaacacaa aaatatataa atataggttg ttcttgtatg atgaataatg acaatcgtat
     7081 tgaattatta gatagaataa tagatgaata taatgtagat gctgtaatag atgtagtttt
     7141 acaagcatgt catacattta atatagaaag ttatagaata agagaatttg ttacaaaaga
     7201 aaaaaataag ccatttatgt ctcttgaaac tgattattca aaatcagata ctgaacaatt
     7261 aagaactaga tttgaagcgt ttgtagagat gttgtaggaa taaataaaag taggaggaac
     7321 tatgtttagt ataggagttg actcagggtc agtggcaaca aagggtgtgc tatttgatgg
     7381 tgaaaaaatt attaagaaaa taattatacc aactggatgg agccctaaaa gtacatctaa
     7441 acaggtctat gagttgctaa gtagtgaaat tgacaaaaaa gatataaaaa aggtggtagg
     7501 tacaggttat ggaaggggag taatggattt tgctgataag aaagtaacag aaataacttg
     7561 tcatactaga ggtatttact ttttaaacaa gaacataagg actattttag atgtaggtgg
     7621 tcaagatagt aaagttataa atttagatag agatggaaat gtatttaact ttataatgaa
     7681 tgataagtgt gctgcaggaa ctggtcgatt tttagagatt acttctaacc ttttaggaag
     7741 cgatattgaa agtatagata cattagctaa aggatatgaa ccagttaata tatctagtat
     7801 gtgtacagtt tttgctgagt ctgaaattgt aagtcttcta gctcaaaaca tttctactgg
     7861 agaggtagca gcagggatac ttaaatctat tgcaaataaa tccacctcaa tgttagcaag
     7921 aggtgaggta atagatgaag ttgcatttac tggagggctt gcaaaaagta aagaactagt
     7981 aaaaatgata gaagaaatac tgggtaaaaa aatttttatt gcagaagata ctcaaataat
     8041 aggagcttta ggagctgctg taataggatt tagataaaaa catataaaag gaaaatgaga
     8101 agctgaattt attataaata aattcagttt tttattttat tttatttatt tataaaggaa
     8161 aactttggta taattgattt aaatgattgt atatttgtaa atacttgata tttagtcata
     8221 tataacatta taattttaaa aattagctca aaataagggg gagaaagcgt gaaaaaattt
     8281 acttcaaaaa aagtaacaag gtttgttgcg ctgtttttaa tatctatttt agtaatgtca
     8341 acaattcctg tatcagctga caatagcaat acattgaatc agataaaaaa agcagaatac
     8401 agtgagacgt ataaacaata tctaaatgat gctaaaaatg gacgtacaga aaaatataat
     8461 gggattatac caaatccata tcagttggaa ggtactcaaa tccaaagtaa aggaagaaca
     8521 gctccaacat cttatgaccc tagaaaatta ggtcttatga catcaataaa aaatcaagag
     8581 gatttaggaa tctgttggga ttttgctgca atggcaacct tagaaagttt cttaaagtta
     8641 aataattatg gagactatga tttatcagaa gaacacttaa gatggtgggc ttcagatggt
     8701 gaatatggat ggtctgtaaa tgatatgaat ggtgctttaa attatgaagc tatgggatat
     8761 ttaacatctt ggtcaggtcc taaactagaa aaagatattc catacaatgg tagagtatct
     8821 aaagcacaag gagcaaaaaa accaactaat atgaatacag cacctactgt ttttaatgtg
     8881 acagatgctg tgtgtgtaag taatgacata aattctacaa aaaatgctat attacagtat
     8941 ggagcagtaa catctggtta ttatgaagat attaaatatc aaagtgatga ccaaaattca
     9001 tattattgtc caacaaaaag ttttaataca aatcatgcta tttcaattgt aggttgggat
     9061 gataactatt ctaaagataa ttttaatgca aatattagac catcaaaaaa tggtgcttgg
     9121 ttaataaaaa atagctgggg agattataat tctgagggtg gatatttctg gatatcttat
     9181 gaagacaaga cacttatgtc tgatatagat aatttttcaa tcaaaggtgg taaaaaacca
     9241 aatgatgatg agaagatgta tcaacatgac tatgcaagta ttgtaccttt aatttcaaaa
     9301 aagataacag ctgcaaatgt atttgatttc aatagaggcg atgaaactct aaaatctgta
     9361 atgttcttaa ctgaaagtat aggagctaag tatgaagtat attatgctcc agtagtaaat
     9421 ggtatacctc aagaaaacaa tatgaaaaaa ttaaaagaag gtactgccca atattcagga
     9481 tacataacag ttccaattga ttcttttgat ataccagaag gaaagggtgc aattgtagtc
     9541 agtatagagg ggaaaaatgg agagtcaact ataggttctg agtcaaatgt accaggatat
     9601 gatacattta aagcaaaagc taatttaggt gaaagttata tcatagataa tactggaaaa
     9661 ttctttgata taaatagaga tagtaacttc tatccgtgta attttactat aaaagctgtt
     9721 acagaaaagt cttctggtga gagtatccca aatgagtctt taattggtag tgatagatat
     9781 gaaactgcta tcaaagttag tcaaaatggt ttcaattctt cagaaaatgt agttttagta
     9841 aatggcagtt caatagtaga tgcattggca gcaactcctt ttacatctgc tataaattca
     9901 ccaatattat tgacacaaaa agaagcttta aattcaaaaa ctaaagctga gatacagaga
     9961 ttaggtgcta agaaagtata cctaataggt ggagaaaatt ctataagtaa agaaattgaa
    10021 caacaattaa aaagcttaaa tatatcgata gagagaatat caggaagtga tagatacaag
    10081 acaagtttat tattagctca aaagttaaat ggtataaaaa atgtatctca aatagcagtt
    10141 gtaaatggag tgaaaggatt agctgatgca attagtgtag gcgctgcagc agcagaaaat
    10201 aacataccaa tcatacttgc aaatgaaaag agcgaattgc aaggagctga tgaatttcta
    10261 aattcattaa atatagagaa atcttatatt attggaggaa cagcatccct atcaaacaat
    10321 ctagaaagta agttaaaaaa tccaactaga ttatctggta gtagtagaga cgaaactaat
    10381 tcaaagataa tagataattt ttataaaaaa gatactttaa aaaatgcatt tgtagttaaa
    10441 aatggaatca agaatcaaaa tgatttaata gatggattgg cagttggtcc tttaggagca
    10501 aaaactgggt caccagtaat tttagttggt gataaattag ctgatagcca aaaagaagtt
    10561 cttaaaaata agactttaga aaaagtaact caagttggtg gaggagcaaa taaaaatgca
    10621 tttaaagagt tagtaaaatt aaaaagtgct aaatagagta cttaagtatt aacatatatt
    10681 agtgtgtatt agtaacttat cttaataaaa gctgaaaata attacttcaa attattgata
    10741 aataagataa tcaatgattg taaagttttg ttttcagctt taaatttatg tagtataagt
    10801 ttaaaaacat aaaaaagggt tactttatat agaaatatag aaaatcttac attttatata
    10861 taaataaccc atatttaatt aactttttat agtgttctaa tagatattat tgaacttaat
    10921 ggtaaatata atgtgtaagt ttcatttaaa gttggattag attcatctat atatcttgag
    10981 caaagtgtaa ttgataagct atctgcttct ataatttcac catatagagg acaactgcta
    11041 aagtaagaat gtacttcaac atatttattt aagttttctt gaacataatc gtaaaaacgg
    11101 ttttttgtag tagtagccat aaaatcaact cctttatttt ttattaaagt tgaaactttt
    11161 aaaaagataa atatgattac ttctattaga aactaagtta aaaaataagc aaatagatat
    11221 gaaaattaga ataatatatt atttatatct taattataac actagataat aaaaaatgct
    11281 atataaaatt cagaattttc gaaagttttt aagcttattt ttaaatatac taataatact
    11341 attgacatta gatagaaaaa gtattatatt gactatataa attattttag tcaaactgga
    11401 gggaaaagat gcctaaatcg tattctgata aagaaagaga gtatataata aaaagactta
    11461 aagaagaagc aagattgtgt atggagcaat atggtattag aaaaactact gttgatgagc
    11521 ttgtaaaacg ggtaaaaata ccaaaaggaa ctttttatct attttttcaa tcaaaagaat
    11581 tgctcttttt tgaggtatta agagatatac atgactctat acaaaaagaa atcttatatg
    11641 agataaatag agtagatgaa agtattacat gtgaacaatt gacagatata tttatgaaat
    11701 tctataggat ggttgatagt acttcaattt taaatcttat gataaatgga gaatttgaga
    11761 tattggtgag aaagttacca gattctataa ttgaagaaca ctttagacat gatgactttg
    11821 aaattggaga gattatatct tgcataccaa atgcaaaaaa taaagacata gaaagcttta
    11881 gtggagcatt tagagcagtt tttcttacca tgctatataa gcgcgaggtt ggaagtaatt
    11941 gttttgaaga tgctttaaga ttaatgataa acggattagt tatacaactt atggaataga
    12001 atttaaattt tgtttgctaa taacttttat taaaaattat taacaaacaa aaaaattata
    12061 ttttattgac catatagtca aaatagtcaa aaatttgaaa gggtgatatt gaatgattaa
    12121 agttgatgat ttatcattta gttatacaga gagagatttt ttacaaaata ttaactttgg
    12181 agtaggaaaa ggggaaatac taggattttt aggaccatca ggtgcaggaa aatctacatt
    12241 acaaaagatt ctaattggaa tgataactaa ttatggagga agtgtcattg taaatggagt
    12301 agagagtaaa agacactcaa ataaatttta tgaaaatata ggagttgatt ttgaatttcc
    12361 tagtttatat gaaaagttga ctgcaataga aaatttaaaa tattttgggt ctctatattc
    12421 taaaaaacta ctatctattg atgagttatt aaaatcagta ggactagaaa atgaagcaaa
    12481 taaaagggta tctgagtatt caaaaggtat gaaatctcga ttaaatttta taaaagcttt
    12541 attacataat ccagatattt tatttttaga tgaaccaaca agtggtcttg acccatcaaa
    12601 tagcaaggtt atgaaagata taattttatc agaaaaatct aaaggtaaaa ctattatttt
    12661 aacaactcat aacatgttag atgcaacaga gctttgtgac agagtagcgt tcattgtaaa
    12721 tggtaaaata tctgctttag atacaccaca taatcttatt atgtcaaaag gagctattaa
    12781 agttagatat acttactttg ataatggaga aaaaacttct gaatgttttc tgaacaatac
    12841 tgctaatgat aaaaatttga atatgcttat tgagaaaaat aaacttttat caatacatag
    12901 tagtgaacca acattaaatg atatttttat tgagataaca gggaggaatt tacaatgagg
    12961 ctaaaaaatc ttattttagg agatataaag tttcaattta agtatggttt ttatttttta
    13021 tatctttttt taagtattat atatatttgt attatcaatg tatttccaac ttttatgaga
    13081 gaaaaaatag ctataattat gatttattct gacccagcag caatgggatt gttttttatg
    13141 ggagctattg tacttcttga gaaaagtcaa agggttttaa attccttagc tgtatctcca
    13201 gtaaaagtat ctgagtatat tttatcaaag gtaatttcat taggagtaat atctagtatt
    13261 gtagctatgt ttattgctat tacattaaat ttagataata ttataatatc aactataggt
    13321 accttttttt cttctattat attttctttg cttggattga tacttgcaag taaagcatct
    13381 agtttgaatc agtttattgt tttaagtatt cctattgaga ttatatgctt tattccacca
    13441 atactcaatg tacttcttga cacaaaatca tatgcaaatt tgtacccttt taatatatgt
    13501 atttctctta ttagtggaga taaaaatttt ataatgatta atattttaat tttaatatca
    13561 attataataa taatttattt tataacttat tattttattt gtagcagttg gaaaaaggta
    13621 ggtggagtaa aactatgata aagattataa atgcgtttaa acaagagttt gaacaaatta
    13681 agagagatgc tatgcttttt atagtatgtg tttctccaat attatgtgga gtatttataa
    13741 aatttggaat accattaata cagaatatat cacttaacaa gttttattat caattaaatt
    13801 tagagccgta tttcttgatg tttgatttac ttttagcttt tattacacct tttatgttct
    13861 tttttgcttc tacaatggtc atattgggag aaattgatga cagcatttca agatatttaa
    13921 tagtaactcc gcttggtaaa actggttatc ttatttcacg atttggaata ccaggtatct
    13981 tagcatttat tattactatg attctactta tctttttttc tctaactaaa atttctttct
    14041 tgttaaactt atcaatttct ttattagttt tattacaagg aattattata tcaatattag
    14101 tgatttcttt atcatcaaat aaactagaag gaatggtaat tacgaaattc tcaggtgttt
    14161 ttatgatggg tattttagca ccatttttta ttttaaataa agcacaatat attctgttct
    14221 tcttaccaac tttttggata tcgaaggcat ttaaggaaga taattatgtc tatatgttta
    14281 tttcaattgt tatatcatta atatggattt tattattatt taaaaagttt agtaaaaaaa
    14341 tagttaaata agaaaattta atatataaat gtaaggttga cttgacagta atgttgaaaa
    14401 atgttacatt taagatgtaa agttaacctt acatgaatgg agcaaatatg aaaaataaaa
    14461 taaagatatt aagagaaaaa ttaggattga cacaagaaca actagggaga ttagttggaa
    14521 catctagaca ggctataaat gctatagaaa ctggtaaaaa tgaaccttca atatggttgg
    14581 catatgatat ttcaagaatt tttaatgaac caattgaaag tgtattttta tttgaagaaa
    14641 gtgaaagaaa atcaagagca cagataagta ggggagaata ttatggctat aagaaagata
    14701 agaacatttg atgatgaaat tttaagaaaa aagagtaagt atgtagaaaa cgtagataat
    14761 aagataagag aaatattaaa tgacatggca gagaccatgt acaatactcc aaatggagga
    14821 ggattagctg catgccaagt aggagtatta aagcgattag ttgttataga tttaggagaa
    14881 ggacttatta agcttgttaa tcctgaaatt attaaacaag aaggggaaca aattgttgtt
    14941 gaaggatgtt taagttttcc tgaagtatgg ggtaaattaa aaagaccaaa aaaagttaca
    15001 gtgcaagctc taaacgaata cggtgagaaa atagaaatta aaggttcagg ttttatggca
    15061 aaatgtcttt gtcatgagat agaccattta aatggtatag tttttacaga taaaattatt
    15121 gaacatgtaa aattgtaaga tagattggtt attgaattta taaaatattt tatagtaagg
    15181 agttaaatta tgagattatg gcataaggat ttaattgatg tattacctaa aaatcaatta
    15241 gtaagtcaat ggagagagct acttgctata aaaggttcta tagataaaaa aggtactcca
    15301 aaccatctat tagtgaataa agtattgaat tatagtatag atgaatttaa attttataca
    15361 aaaattgtac atgatgaaat gttaaaaaga aattataaac ctaatgaact taaatataca
    15421 agtatattaa aatggaaaaa tagaaatttt gcaaatgata tatctaatga acatagttta
    15481 aatttagaaa atttatatga tgattggcat aataaaatgt atttaaagca atgcttatat
    15541 aatttagaag aaaaagcaac atgtggagga ataccaataa atgaatggaa tatattactt
    15601 tgtaaatata gtaaagatta tgaattgtgg tctggaaata tcatgtttta attactatag
    15661 attaacatta tagtattatt gtatataaag aggtgattat ttgtataata aaataaaata
    15721 tattccaata aattgtgaaa tagcttgtaa taaattaaaa aggagtatgc catataaatg
    15781 ggatttaaat atttatcgtg gatgtgagca tggatgtaaa tattgttatg ctatttactc
    15841 tcataagtac atcaactcca ataattattt tgaagagatt tatgtaaaaa caaatatagt
    15901 agaaaaactt gagagacaac ttaaaagcag taaatggaag aaagaagtaa tcaatatagg
    15961 aggagttaca gatagttatc aaccaataga agctgactat aaaattatgc cagaaatatt
    16021 aaacctgcta ataaaataca agacaccagc tattatttct acaaagtcag atttaatttt
    16081 aagagattac gatttaattg ataagctatc taggattaca tatatcaatg tagcatctac
    16141 aattaccaca gtaaatgaag atacacaaag gcttattgag ccaaatggtg tggattcaat
    16201 gagacgattt aaaatgttaa aagaatttag aaaaactaac gcatcagttg gtctacatat
    16261 tatgccaatt ataccatata ttacagatga ttttgataat ataaattcgc tatttagaca
    16321 tgcaaaagaa tgtaatgtac attatgtttt acctgggact ttatacttaa ggggtataac
    16381 tagaggtgtt ttttttgact ttgttaaaaa agagtttcct gaattatttg ataaattatc
    16441 aatactatat agtgaaggtt cacttaatag ggagtataaa aatcaattgt ataaaatggt
    16501 taatgaactt agatataaat attctctatc aagcagttac tctaaaataa tgaaggaaaa
    16561 gttaaaaaac tcaggagatg tacagatatc tttttttgat tagttttata taaaaaaatt
    16621 ttttttacta tgat
//
LOCUS       DAMJZE010000087        15978 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJZE010000087 DAMJZE010000000
VERSION     DAMJZE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.088"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC541_002303"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC541_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2839763.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC541_002304"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC541_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2839764.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC541_002305"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC541_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2839765.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="PC541_002306"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="PC541_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2839766.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="PC541_002307"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="PC541_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2839767.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="PC541_002308"
     CDS             2662..3168
                     /locus_tag="PC541_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2839768.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="PC541_002309"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="PC541_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2839769.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="PC541_002310"
     CDS             3982..4770
                     /locus_tag="PC541_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2839770.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="PC541_002311"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="PC541_002311"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2839771.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="PC541_002312"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="PC541_002312"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2839772.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="PC541_002313"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="PC541_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2839773.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="PC541_002314"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="PC541_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2839774.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="PC541_002315"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="PC541_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2839775.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6257 a         1989 c         3249 g         4483 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAMJZE010000088        15837 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJZE010000088 DAMJZE010000000
VERSION     DAMJZE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.089"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            54..650
                     /locus_tag="PC541_002316"
     CDS             54..650
                     /locus_tag="PC541_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2839776.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(748..1707)
                     /locus_tag="PC541_002317"
     CDS             complement(748..1707)
                     /locus_tag="PC541_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2839777.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1741..2472)
                     /locus_tag="PC541_002318"
     CDS             complement(1741..2472)
                     /locus_tag="PC541_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839778.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2465..3235)
                     /locus_tag="PC541_002319"
     CDS             complement(2465..3235)
                     /locus_tag="PC541_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839779.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3320..4030)
                     /locus_tag="PC541_002320"
     CDS             complement(3320..4030)
                     /locus_tag="PC541_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2839780.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4210..4362)
                     /locus_tag="PC541_002321"
     CDS             complement(4210..4362)
                     /locus_tag="PC541_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2839781.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4388..6544)
                     /gene="feoB"
                     /locus_tag="PC541_002322"
     CDS             complement(4388..6544)
                     /gene="feoB"
                     /locus_tag="PC541_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2839782.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6644..6865)
                     /locus_tag="PC541_002323"
     CDS             complement(6644..6865)
                     /locus_tag="PC541_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2839783.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6878..7090)
                     /locus_tag="PC541_002324"
     CDS             complement(6878..7090)
                     /locus_tag="PC541_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2839784.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7411..10308)
                     /locus_tag="PC541_002325"
     CDS             complement(7411..10308)
                     /locus_tag="PC541_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2839785.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10635..11243)
                     /locus_tag="PC541_002326"
     CDS             complement(10635..11243)
                     /locus_tag="PC541_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2839786.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11503..12048)
                     /locus_tag="PC541_002327"
     CDS             complement(11503..12048)
                     /locus_tag="PC541_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2839787.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12166..13989)
                     /locus_tag="PC541_002328"
     CDS             complement(12166..13989)
                     /locus_tag="PC541_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839788.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(14002..15777)
                     /locus_tag="PC541_002329"
     CDS             complement(14002..15777)
                     /locus_tag="PC541_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839789.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5255 a         2840 c         1563 g         6179 t
ORIGIN      
        1 tagtataatc taattacttg atattaattt tcatttgaag gaggttatct tttatgtctt
       61 gttgtaattc acaattttgt tgtaaaaata aaattgaatc atcatatata aaatggcttt
      121 tagaagtttt aggtccagtc tttttaggct ctaaacctgc tgaaataata aatatttcgt
      181 ttactgatgt aaatagagag gaaaaaatta atgatattta taaatatcta tataagtgta
      241 agaaaataga ttttatagtt attgataaag aaaaaaaggg attaaaaatc ttatttgtaa
      301 ataaaaaagc attatcagaa aaattaaaat gtaaaaaaac agtaaacttt ttaaagtttt
      361 taggatacaa acaaaatacg aatgttaatg cttatttaga gcatttagta gataaactta
      421 agagtgatgt ttttcctgat gaaataggta tttttttagg atatcctctt aaagatgtga
      481 ttggatttat gggatatagt aattatgaag tttctatgat taaatattgg aaagtttatg
      541 gtgacacaaa acaatccgag gacacatatt cgaagttttt acttcaccgt aagaaaatga
      601 gaaaattact agactacata agtgtagata aaatagtaag ctgtttttaa ttgtgttttt
      661 gtataataat aaaaatacac cctcttttac catacagttt ccataataaa taagggtgta
      721 ttttccactt taatatttta aagtattcta attttgattg tacaacatat ctttaatatt
      781 tattttcttt tgttgcattc cattagacat caaaaattcc tgtgtatctt ctaattcttt
      841 tatatccttg tccgttattt tagaactaaa gtcataccaa gaatacattt cttttgtttc
      901 atctaaagat aaaccaactt ctttagatac aacatccatc acttcatcct tgttttcatt
      961 catatatttt aaagtttctt catgaacctt cataaatctt tttactaatt caggatattt
     1021 ttcagcaaag tcatcactta cagcagtaac tattattcca cttactaatc cttctccatt
     1081 tacaatcatt ttggcacctg aatttatcgt tttcaatgcc actggacctg caagcattgc
     1141 tacatcagca cttttacttt ctaatgcaga agcagcacta ggaatatcca tatttacata
     1201 ttcaacatca tctatagtat agccttcttt agcaagtgca gcaatcaaca gttgatgaag
     1261 tatagtccct tttggaccta caattttttt accttttaag tcttttatag atttaatatt
     1321 atcattatta gttataagca tatatccttt tggtgaacgg ctataaatat tcgtaatttt
     1381 aagaccaaca ccatttgatg cagctattat tgcagacgtt cctcctaatg catgtaaaaa
     1441 gtctagctca cctgctgcta atgcttgagt ttgctctggt cctgttgtca attcatggaa
     1501 attgactttt atattgtcct ttttaaattc ctttccaaat aaatcatcct ttttttctaa
     1561 tatactagga acattaagtg gtgattttac ataagttaaa tttatttcct taggtaaact
     1621 agcctctttt tttgcaggtt catctttctt atcatttgtt gttgaacatc ctacagtccc
     1681 aaccaacacc attattaaag atgtcattac tgctaaaatc tttttgtttc ttttaatcaa
     1741 ttaaaactcc cccaattccc ttaatatttc ttttttcaag tttatataat agtttttagt
     1801 taaatctcta ttatattcat cttcgacact aaattgttta atccttttat ttttactaaa
     1861 aacaattatt ttctttgcta tttccatagc ttcttcaata ttgtgagtta caaatactac
     1921 acctttttta gtatttttat ggatatttac aacttctttt tgcatttttc ttcttgtgaa
     1981 gtaatctagt gctgcaaatg gttcgtccat cagcaaaata tcgggattga aggaaagtgc
     2041 tcttgctata cttactctgt gagccattcc tcctgataat tcatttggat atgagttttt
     2101 aaacttttca agcttcatca tttttaaata cttgtctaaa tcaactttat ttctattatc
     2161 tttttcagta tgtaaaagta tattttcact tacattcagc catggcataa gtctactttc
     2221 ttgaaatacc attcctactt ttggagtaca ctttttatca ttattaaaaa aatatacgtt
     2281 tccactagta gcattttcaa gccctgctaa aattctaagt agcgttgttt tcccacatcc
     2341 actttcacct aatataacag ttatctcttc actagatata tttaaagata tattatcaag
     2401 aactaaatgc tctttattat caactaaata tttcttatat atattctcta gcaaaaatcc
     2461 acttctaacc atttaaatca acctgcttcc ctttgctaaa ttttgttatt atctttgaaa
     2521 atagataatc tgaaagtata cctaaaaatc caataacaaa aattcctacc aatacaacat
     2581 cagttctaga catatctttt ccatcagaaa tcaaatatcc tagtcctgaa gaagctgcaa
     2641 taagctctgc tccaattata gccctaaaac tatacccaag ccctaaacta agtccaacaa
     2701 caatatccag catagaatta gggattatta ttttataaaa aattctcttt ttagataaat
     2761 taaaagattt acctacttct attaatttat tgtcacaatt ttttatacct ttcaatgtat
     2821 ttaaaaatat tggaaaaaag gatgccaaaa taataattat aattttagat tcttcaccta
     2881 taccaaacca taaaataagc attggcacca aagctagtgg aggtgtactt cgcataaatt
     2941 caaatagtgg tttgaaatat tcatacattt ttttatttgc tccaaataaa actccaagtg
     3001 gtatagccat caatgaactt attaaaaaac ctattaatat ccttcttaca ctggcataaa
     3061 catttaagaa aatagaaccg tcatatatca tttttataaa agtatctaag actatttcag
     3121 gaggaggcag aatatagcta ctccatatac ccaaaacaca tactatcttc cataaaatta
     3181 aaattattaa aaaaatataa tatcctttaa gccttttatg aattttctca aacatataaa
     3241 actcctttta acatatctat ccctgctacc ctttttattt tttaatatct taaataataa
     3301 ttaagctata tcataagatt taatctatgc caagtcttaa tccacctgaa ctcattctat
     3361 cagaaggtgt agaatctgtt tttaagccag acaatctttc agatggttgt ataatgacta
     3421 tttgtccagg ttcaacaaat tccatatgat aagattctcc tgaagtctga ccaataaaag
     3481 tcttccatga tagttttgat actttaggat atggttctct tccagtccaa gctactaatg
     3541 catctgggtc aacacaacaa ggtccttcaa gaattagagg attaccatct gttattatag
     3601 caacatcttg attttgacct gttttattta ctaaatgtgt agtaaaaaga cctttttgag
     3661 atattacccc tgaaccaatc aattttacac tgtaatttaa ttgttcagaa aatgccaata
     3721 agttttcact ctcaacataa atggaatcac ctgggtcaat tgatattaca tctacatggt
     3781 aagcattttg agctaggtaa atttcacctt caccttcaac caccattatt ggcatttgtt
     3841 ctcctgtcaa tgacctctga acatgaccta ataatgctct tcccaatcca ccgccattac
     3901 ttggacctaa cagcaatttt tcaaaattaa aatttccttt aaaggctacc atagcacctt
     3961 tttttgcaaa aaatttacca cttccccttg ctatacacat aagctcgttt gtaacatcaa
     4021 atttaaacat aatattcact tatattgctt aatttagcaa taatccctcc taatcatata
     4081 tttatatata attatacagt aaaattacag catagtctaa cctacttcaa atatgaaatt
     4141 tgttatttat acaaaaaata agaacctgat aataaagcaa tttaactttt tatcaggctc
     4201 ttatcctatt tatctcttag cgctatgaca actcttacta cttgagcatc cagaacaacc
     4261 acatccacaa ctgctaccat tcttttttga atttttaacc atatacatta cagctagtgc
     4321 cattaatact actacaatag ctgctataat aaatgtagcc atattcatca ctccttattt
     4381 atatgaatta atttttcata taacttaatt tacccatttt ttcttttttc atatttttat
     4441 ttgaataagt taaaactaca aagactactg ctactgctat aaatatagat attattgccc
     4501 cagcaatact tcctccagta cctaaaacta aacttccaac ttgatttata agaagagcaa
     4561 ttatatacgc tgttaaagtt tggaatccta atgtaatcca tgtccatttc catgaaccca
     4621 tttctctttt tatggctcca atagctgcaa aacaaggtgc acaaagcata ttaaatgcca
     4681 taaatgcaaa cgcactagct actgtaaaca tacttgcaac tgaagcaaga agtgttgggt
     4741 cttgctctgt tgcatctgat ataccaaata aaactccaaa tgttccaact acattttcct
     4801 tagctactag acctgtaact gttgctactg atgattgcca atttccaaat ccaagaggag
     4861 caaatattgg tgcaactata tttcctaagc tagctaatat actatctcca gcatctacca
     4921 tttgtaatga ccaattaaat gattgtaaga accagattac tccacaagca acaaatataa
     4981 ttgttcctgc tttgattata aatgcttttc ctctatccca catatgtatt aaaacacctt
     5041 ttgcacttgg aatatgatat tgaggtaatt ccataacaaa tggagatggt tctcctgcaa
     5101 ataaactagt tttctttaat ataattccac aaatgattat cattgcaata cctaagaaat
     5161 acattgatgg agctacccat gaagcacctc caaataatgc ccctgcaaat aatgctatta
     5221 ttggaatctt agctccacat ggaatgaatg tagttaacat aatagtcatt tttctatctc
     5281 tatcattctc tatagtacga gttgacataa ctcctggaac tccacaacct gaacttataa
     5341 gcattggtat aaatgatttt cctgaaagac caaacttacg gaaaattcta tccattataa
     5401 aagctacacg tgacatgtac ccacaatctt ctaatattga taataataag aataaaagca
     5461 tgatttgtgg tacaaatcct agtactgctc caactccacc aattagacca tcaactacaa
     5521 gtccttgtaa ccactctgct acatttaaag aagctagaaa attacttaca ttgccttgaa
     5581 taatttctcc aaacagtaca tcatttgtcc aatcagttgc tatagtaccc aatgaactta
     5641 ctgctatata gtaaactccc cacattataa gtgcaaaaat aggtaatgct agtatacgat
     5701 ttgttacaat cttatctatc ttatctgata cagtttcctt accttttcga ttcttcttta
     5761 ttatacttga aattattgaa ctaataaatt cataacgatt ggctgttatt atactttcac
     5821 tatcatcatc caattcatct tcacaatttc tagttatttc ttcaatacta ttcaatattg
     5881 ttttagaaat atcaagtttt tgtataacat tttcatctct ctcaaatagc tttatagcta
     5941 accagcgtgt ttctacatct atatatggag tcttttcttc tattattttt tcaatttttt
     6001 ctattgcatc cttactttca tcaacaaagg gtaatttgaa atttaattta ttgttactac
     6061 ttgcaagttc aactgccttt tcagcagcct ccataattcc ttgaccttta actgctgtta
     6121 cttctaccac tgggcaacct ataacttcag atagttcttt aatatttatc ttatcaccat
     6181 tcttatttac aatatccatc atattaagag ctataacggt cggtatacct agctctaaaa
     6241 cttgagttgt aagatataaa tttctctcta tatttgatgc atctacaata tttattactg
     6301 catccggctt atcatctagc ataaaattac gtgtaactac ttcttctaat gtatatggag
     6361 ataaagaata aatacctggt aaatctacta tttccacatc tttatttcct tttaattttc
     6421 cacctttttt ttcgactgtt actcctggcc agttaccaac atattgtgat gagcctgtaa
     6481 gcccattgaa cattgttgtc ttaccacagt taggatttcc tatgagacct atttttattg
     6541 acatttccat tccctccatt attatttaaa tacatcactg tttgtgatac agaaaatcat
     6601 tctcattata taattacatt aaccctactc taattaatct ttattattcc acaataattt
     6661 tttctgcatc tgacttacga attgatagtt cataatctct gactgttacc tcaattgggt
     6721 caccaagagg agctactttt cttataaata tattaacacc tcttgtaatc cccatatcca
     6781 ttatacgtct tcgtgtagct ccttctccct ctaacttttt gacttttaca gtctctccac
     6841 acttaatatc cttcagtctg ttcatgtcat tacctcccta tattattatt ctacttgcca
     6901 tttttttatc taatgcaact ctagtatctt ttacgtctat tattaagtta ccatctatct
     6961 tagaaactat tttaacattt gtacccacta caaaacctat actatttaaa aaagtcctcg
     7021 tcttatcact tcccgaaatt ttctttataa ctacttcttc acctgtattt gataatatca
     7081 atggcatcat ataatatccc tcctcttaaa gtaatttaaa tctacaagca atcgttatct
     7141 tatatataaa ttacaatacc ttaaatctta atctattaaa ataacatcct attttttcct
     7201 tctttttaca attcctaatt ttgatttaag tctatcacaa tagtatattt tttgcaagtg
     7261 attattgtat tcttttgatt ttttttctca tttgatttat tttattaata aaatcgtaaa
     7321 aattaataca aaaataaaga tgctctaaaa tagtatactc aactaatatt aaagcacctt
     7381 tattatatat gtcattattt acacattgct ttattctttt ttaaaagcga ttttttcaaa
     7441 ctcttttaca ggcattggct tagaaaacaa atatccttgt accatatcac acccaatttg
     7501 ctttagaaat tctacttgcg aaatagtctc tacaccttct gatattactt tcattttcag
     7561 ttcctttgcc attttaatga cattagatat aacaattttt tcattattac tctcatcttt
     7621 ttctgtaaaa aatgctctat ctagttttaa tatgtcaaca ggcaattctt ttaaaagatt
     7681 aagagaggaa tatcctgtac caaaatcgtc catagagatt acaaatccct tttctttcaa
     7741 attgttcatt gtatcaagta atccttctat attatctaat actgctgttt ctgttagctc
     7801 taattctata agtcttgtcg gaattttgta tttatctaca attaatttaa actcctctat
     7861 aaaattacta tttacaaaat gcattcgaga caagtttaca gatataggta ctactttttg
     7921 tccttcatcc atccattctc taatcttttt acacacttcc tcaaatacat agaaatcaat
     7981 atttacaata aaaccattcc tctcaaatac aggaataaat tctattggtg gtattagacc
     8041 tttttgtgga ttatcccaac gaacaagagc ctctgcccct ataatttgat aatctgaaag
     8101 gctatattta ggttgaaaat aaacaataaa ttctccatta ttaagagcat ctaccattga
     8161 attttcaatt tctttctctt ttaatatttt tttgtgcatt tccttatcgt aaaaagcaaa
     8221 tgaattttta tgaccacctt ttatagtttt tcttgcagta ttagctctat ccataatagt
     8281 atttatgtca ttatcacttt tacttatttt atatatacca caatctaaaa tcaatttata
     8341 atagtttatt ttaggattac taagtatttg aaccttattg taaatattgt ttaaccttgt
     8401 tttaatatca tcatctgtct tatacttaat aagaagaaca aaatggtcag cagaaactct
     8461 tgcaaaaatt tcatcttcct ttaattcact tgcaatagta tttgatatat gtatcaaagt
     8521 atcatttccc atatcatatc caaacatatc atttatatat ttaaatttgt caacatctat
     8581 ataaaacaac gcatattctt ctggattatt cttagcaaac agtttattag aatttatttt
     8641 aaatttatct atactatctg ctcctgtcaa tgggtctgta tatgctatct tccttagata
     8701 atcctctttc ttctttttat tataaattat atatttaata gcaattaaga ataaaataca
     8761 tacaaaagaa ataatttgta ttgaatattc tttaaaaatc acaccaaatg gtactttata
     8821 aggtttgtca atagtattag aatatataca atcacttaat tcttcttcag taattttgtc
     8881 tattgattta tttaaaatag acactaatat agggtcagtt ttatttgata cccctatacg
     8941 catcccatag cttgtatcta aaacagatat aacagataaa ttctcactct ttccttctct
     9001 taaaagttca tcaaactgat aagaactaag tatcattaag tttgctttac cttcatttat
     9061 agcgtttaga caactttcta cactcttata agatgttgtc ttagcatttt caaaactctt
     9121 ttctatataa gaatcaatag acttataact actagttaaa gcaatattta aatcattatt
     9181 gagattggta tccaattttc ctaccataat cattggaata tttatgtatg gatttgtcaa
     9241 tttcatatta aatcgtttta ccttatcatt ttcattgatt gagccacata gcatagttgc
     9301 cttaccactt tttatgtaat caagactctc attataattt tttgttttaa tatattcaaa
     9361 ttttatacca catttttttg atataattga cattaaatcg gaacttatac ctttaaatga
     9421 attgctttct tcatcataat actctacagg agaccactct gaatcatata ccatagtaag
     9481 ttttgggttt gctttaataa aattcatttc ttgccgagta aatgctatgg aattattgac
     9541 ctctcttttg aaatattttt tatataattc caactcataa tacatatcat ttatttttaa
     9601 ttcttttaaa gcataatcta attcttttac taaattaggt ttatcttttg cagtagcaaa
     9661 ataaaaaggt tctactgaaa atttgctaac tattttcatt cctttagcat ttataatact
     9721 tccacaaaca aatgcatctg cctttttttc attgacggac tcaattaatt catcaataga
     9781 attacagtat atatattcaa ctgaaaaatt attttcttct ctatacttct caaatgcagt
     9841 attatgaaag tttcctctaa gtacagctac tctcattccc ttaaaggaac ttatatcttc
     9901 ataaaacata ttattgttac tatcctttgt atatagtgca ctatattcat atcctgaact
     9961 tagtttagga aaattaaata gtttatttct ttcatcattc ttctgtaaag gtccgagtaa
    10021 gtctatttca cccttttcta gtctgtctaa acattcttcc caagaccctt ttacaaattc
    10081 atattcccat cttgtatatt ttgagatttc tcttagataa tcataaccat atcctgtata
    10141 agaatgctct tttgatgagg atattattcc gtagtcatca caatatccaa ctcttatttt
    10201 ttttgaattt tcagactcat cagcaaagca tagtatattg ttaaaacatg atataataag
    10261 taaaaatatc aaagaaagta tcaagaagtg ctttttaaat gctttcatat ttaaaaacct
    10321 ccaatttgcc aacttacttt aacttatttc gttttctagt tgtaactact tttattgtaa
    10381 tggtaaatat atcaaattgc aatattctca tataatattc ttattcaact ataatgaatt
    10441 tattattatc tatttaaata cactatctaa tattagtttc ttataagtca taaaagcaca
    10501 actaacacta taattctagt gattaatttt atttcaaaaa atataataac actactaata
    10561 ttttaaatat caatagtgtc ataaaaactt ttcagattaa ttaaatcttt ctatatattt
    10621 tataataatc taaatcatgc taaatattta gacagaaaac ttttttcttt aatagtaaat
    10681 acttttgttt gaatatcaga ataattattt gaatatctat ctataaaacc atgatttgca
    10741 tctaaaatct caatttggat attttgtttc ctagaaagtt ggtaagaaat agaatcaaca
    10801 tcaaatgaat cgtgctttgc aaatactaat aaggtaggac attttggaat tacatccatg
    10861 taatctctaa tccgagagcc ataacaacaa attatgccat cacaaaaaga atcttcactg
    10921 catctccatg caatagttgc tcccacgcta tacccagcaa caaatacata tttatactct
    10981 gattttaaat catttataag cttttcaact tttttataaa tatcaaaacc aacattattt
    11041 ataaaaaaat catatgcctc ttggactttt gaatatggaa aaactctttt ttctcccaga
    11101 agttcaggac aaaatacatc atatccatat ttataatact tttgacaaac atcttctata
    11161 aagctattaa tcccatagat ttcatgcaaa acaacaatag ctacatcact tttatgtata
    11221 tactttatca gattatccac cacctttaca aaacagcaaa ttttttatac ttagtttaat
    11281 atattcttct ttacatgtca atttactttt ctaaaatcaa atttattttt ggattaatca
    11341 tttattttta aaagttgtgc attattacta ttaaattatt ctctattatt aaaaatataa
    11401 tcgttagaaa taaaaaataa agtacaaata attaaaaaat atctcactca taactttaaa
    11461 aattataagt gagatactcc taaaatctac tttatattaa atttattttc caaaatatct
    11521 atttaataat cctaaaaatg ctttaccatg tctagcttca tctttacaca tttcatgtac
    11581 tgtatcatgt atagcatcta atcctaattc ttttgctttc ttagctaatt ttaattttcc
    11641 atcagttgca ccatactcag catcaactct aactcttaag ttttcttttg tatctgcaac
    11701 tacaacttct ccaagaagtt ctgcaaattt agcagcatgt tcagcttctt caagagctat
    11761 tctcttataa gcttctccta cttctggata accttctcta tcagcttgtc tactcattgc
    11821 taaatacatt cctacttctg tacactcacc aacaaagtta gctcttaatc cttcgattat
    11881 ttcttcatct acaccttgag ctactcctat tctgtgctca tcagcccaat ttttttcgcc
    11941 tttcatttct tcaaatttat cagctccaac tttacatact ggacattgtg ctggtgcagc
    12001 atctccttca tgtatatatc cacatactgt acaaacaaat ttcttcataa ttactttcct
    12061 cctaaatttt atatatcaaa ttaaattttt tatttattaa gttagtctat atatacccgc
    12121 accatatacc tttaaacaat ttttttaaaa gttttttcta aatacttatt ttccctgtgt
    12181 caataatatg tttctataat aatgtccctt taattttatc aattctttat gatttcctga
    12241 ttccacaatt ctaccatttt ctaaaactat tatattatca caatttgata tggtacttaa
    12301 tctatgagct ataattatac ttgtctttcc ataagttaaa ttatttattg cttcttgaat
    12361 tttagcttct gttgctatat ctatgttact tgtagcctca tccaaaataa gtattggagc
    12421 attttttaat actgctctag caatacttat taattgtttt tgaccttcac ttaaatcttc
    12481 acccctatcc gataatacag tactatatcc ttcacgcatt ttaactataa atttatgtgc
    12541 atttgctatt ttactagcat atataacttc ttcatcagta gcctccagat ttccatacct
    12601 tatattttcc ataactgtat cattgaatat ataaccttct tgtggaacta ctccaaaaca
    12661 agttctcaaa tagtttctgt taatagagta tatatcctta ccatctaaaa gtatacttcc
    12721 ttcatctata tgataaaaat ttgtaattag atttataata gttgtttttc cacttcccgt
    12781 ctctcctatg atggcatttg atgtgccagc tttgattttt aggtttatat tatctaaaat
    12841 tttctttttt gaatcataag aaaaacttac atctttaagc tcaatttctc cctttacaaa
    12901 atgagagttt aaattaccat ctttattatc gttaatctca cttttaaaca tgttatgcac
    12961 ttcttgctct tcttgttcat ctataatttc aaaaactctt tcacatccag ctatggctga
    13021 aagtaatgta ttaaatagat ttgctaattc atttaaaggt cttgtaaatt gtcttacata
    13081 acttaaaaaa ctagctataa cccctatggt tataatatct ttagatgcaa gaatgcctcc
    13141 tataatacat atagttgcaa atcctatatt actaatcaca ttcataatag gcattaaaag
    13201 agaagtgtaa atttgcgatt taagggaaat atttagaatt ctatcattga cttctttaaa
    13261 atcatttaca gattttcttt cataattaaa agattttact acatttatgt ttgatatcat
    13321 ttcttcaata tgtgcattta gttttcccaa ctcaacttgt tgttgcttaa aaaaaactct
    13381 agttctcttt gcaataaaat tactcacaaa cattactatc ggtattaata ttacactgca
    13441 aaaggttaaa attgggctta atacaaacat cataataaaa gtcatcaata tgtttaagat
    13501 tcctgtaatt aactgtacta atgaatttga tattcctgta ctgatattat caacatcatt
    13561 tgtcactcta ctcataatat ctccagtaga acttttatca aaaaaggtta ttggtagatt
    13621 tttaaatttt aaaaataaat tacttctcat ttctttaact atactttgag ataatctagc
    13681 tgtacatatc cccttagaca gtttaactga aaactcaatt atataaaatg aaagtaaaat
    13741 aattattcct tttaagaaaa tggattttga ataagtatcg atatctatta tgtcaactac
    13801 ctttccaata gtatatggta ttacagttga tataattgaa tcaattatta ctagcaaaaa
    13861 aataaatcta gtaatcttct tatctttttt aaagtacata aaaattcttt taaacgtttt
    13921 tttaaaatct ttaggctttt cttttttctt taatctactg accttctgat ttccagtact
    13981 taaattcaat atacttcctc cttaatttac atcttcactt actaaattct gcaacgcaaa
    14041 aattttttta taaattggag aactaaccag taaatcttca tgtgtagcaa atccaaccac
    14101 ctcaccctca tccaagacta aaatcttatc acagtcaatt atagaactaa tcttttgtga
    14161 aatagttatt acagtacatt tcttactttc cttttctctt aaattactta tatactcttt
    14221 tatattcttt cttataattg tctctgtatt aatatccatt gcactaaaac aatcatcaag
    14281 aattaatatt tgtggttttt tggctatagc tctagctata gtaattcttt gtctctgtcc
    14341 tcctgataaa tttttacccc tttggaataa aacttcatca taagtatttt cgagttttgt
    14401 tataaattga tttgcatttg ctatttcaca tgattttaca acatcctctt tgcatatatc
    14461 tctatttccc ataactacat tctccaatat actttttcca aacacactac tattttgaaa
    14521 tactacacct attcttgccc ttaaactttc ttcagaaata ctgtatatac tacgtcctcc
    14581 aatcttaata tcacctttat caattatata taatttttga attaaattta ctaaagaagt
    14641 ctttcctgaa ccagtttgac caatcacacc aatattttga ccttcttcta ttttaaagga
    14701 tacgtttttt aatatttttt catctgtatt tttgttatac gaaaaatcta cattttcaaa
    14761 aattatatca cagtttacaa actttaaatc tttttctcca acatctaaac tttcttctct
    14821 ctcaattact tcttcattct catcaaccaa aacctcactt attctctcat aagatgcttt
    14881 tgccctaaca aaaacattaa aaacatgtga aatcattata agagatgtta atattctagt
    14941 catatagttt acataagcaa ctataatccc aattttaatt tctcctgaat aaattaaatg
    15001 ccttccaatt agtaatatta ttattatgga aacattaatg attattgtaa ttgtcggtct
    15061 aaaaaatgac atcatagttg tagcttttct actattttta aaaagttcat catttaactc
    15121 ttcaaagctt tggttctcat aatcgtatct acaaaatagt ttaactactt ttattccact
    15181 taaaaactgt cttaattttg aatttacttt atctgtactt tcttgtacat ttttaaataa
    15241 tctataccct atcttcatat taagcaatat tataataaat ataattaaaa ctgcaattag
    15301 aataattatt gacatcttta aatttaattt tatagctata attaaacttc ctatacacat
    15361 tattggtgac tttataaaaa ctctcatcat tccattaacg aattgttgca tttgattgat
    15421 atcattagta attcttgtta ttaatgaagc tctaccaaat ttatctattt gttttaatga
    15481 gtatgtattt atcttttcaa atacatcctg tcttaaatca taagaaaaat gctgagaaac
    15541 tcttgccgag attacacttc ttattattgc aaatattgca cctaaaaaag ttatacctat
    15601 cattatagct cctgttgata gtatgtagtc taagttctta tttgaaaccc ctatatctat
    15661 tatttttgcc attatagttg gttgagctaa atcacaaaaa gcttccaatg ttaaaaaaaa
    15721 tactgctgtt agaaataata ctatatattt ttttatgtat tttttaaaat acctcattca
    15781 atcatccctt catagaattt tctcattacc aaaagtatat cataatggct aatttaa
//
LOCUS       DAMJZE010000089        15735 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJZE010000089 DAMJZE010000000
VERSION     DAMJZE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.090"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            231..1310
                     /locus_tag="PC541_002330"
     CDS             231..1310
                     /locus_tag="PC541_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2839790.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC541_002331"
     CDS             1634..2002
                     /locus_tag="PC541_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2839791.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC541_002332"
     CDS             2119..2922
                     /locus_tag="PC541_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2839792.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="PC541_002333"
     CDS             3314..4204
                     /locus_tag="PC541_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2839793.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
     gene            4767..5210
                     /locus_tag="PC541_002334"
     CDS             4767..5210
                     /locus_tag="PC541_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2839794.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5382..5552
                     /locus_tag="PC541_002335"
     CDS             5382..5552
                     /locus_tag="PC541_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839795.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5925..7022
                     /locus_tag="PC541_002336"
     CDS             5925..7022
                     /locus_tag="PC541_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2839796.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            7227..7715
                     /locus_tag="PC541_002337"
     CDS             7227..7715
                     /locus_tag="PC541_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2839797.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7801..8208
                     /locus_tag="PC541_002338"
     CDS             7801..8208
                     /locus_tag="PC541_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839798.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8209..8427
                     /locus_tag="PC541_002339"
     CDS             8209..8427
                     /locus_tag="PC541_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839799.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            9584..9889
                     /locus_tag="PC541_002340"
     CDS             9584..9889
                     /locus_tag="PC541_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839800.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            10297..10986
                     /locus_tag="PC541_002341"
     CDS             10297..10986
                     /locus_tag="PC541_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839801.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            10986..11903
                     /locus_tag="PC541_002342"
     CDS             10986..11903
                     /locus_tag="PC541_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2839802.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            11976..12902
                     /locus_tag="PC541_002343"
     CDS             11976..12902
                     /locus_tag="PC541_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839803.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            12899..13579
                     /locus_tag="PC541_002344"
     CDS             12899..13579
                     /locus_tag="PC541_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839804.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            13677..14012
                     /locus_tag="PC541_002345"
                     /pseudo
     CDS             13677..14012
                     /locus_tag="PC541_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            14996..15646
                     /locus_tag="PC541_002346"
     CDS             14996..15646
                     /locus_tag="PC541_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2839805.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT         6254 a         1554 c         2628 g         5299 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata ctctaggtaa ttgctagagt tagtattata gatgaatatt
     4441 agtattgata ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata
     4501 ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata ttctaggtaa
     4561 ttgctagagt tagtattata gatgaatatt agtattgata ttctaggtaa ttgctagagc
     4621 tgatattata atttattatt agtattaata ttctagggaa ttatcactat attttaaaaa
     4681 gtaaaaatat atatcaaaat gaattaaata agtactgtaa gaagtttgtt aaattaaggt
     4741 ttgaataaag tgagggtgga gaacttatga tagaaataaa aactatagaa ataaataaaa
     4801 aaagtcgaat tgacctttta atgttagccg acccatgtga agaaatgatt gataaatatc
     4861 tcaataaagg aactatgtac gcattgtatg ataataaaga acttacttgt atagcagttg
     4921 taaatgaaat ttccaaagaa atatgtgaat taaaaaacat tgctacatat gagcattttc
     4981 aaaatatggg ttatgcctca aagatgattt atcatctttt agatgtttat agtaaaaaat
     5041 attcatcaat ggttgttggt acatctgaaa gcggagttcc tttttatgag aagtttggat
     5101 ttgtttattc acataaaatt aaaaactttt ttgtagataa ttatccagaa ccaatctttg
     5161 aaggagaatt acaatgtgtt gacatgttgt atctatcata taacttttag ataggataat
     5221 atttttaggt taatataatt aattaattca tataattatc ggaattataa tttataaatt
     5281 atgtataata agagtataat ttatttagaa gctgttatat tgtattatat tcaaataaga
     5341 tatataataa cttgtttatt ttgaattttg gaggattaat tatggattca aataataata
     5401 ctataaaatc aactgttaaa aagggtattt cttttggttc ttgtttagca atgattattt
     5461 cttatactgc atggaaatct attccatggg ctatttttca tggcttaatg agttggatat
     5521 atgtacttta ttattgggtt aagtatgcat agattaaatt aataaataag tatgtaatat
     5581 tgtaaagttt aagaaacaaa atgtaaggga gtgtctcgaa gtgaactttt agtttataag
     5641 gcactctttt ttgtattaaa taaaatatat tttcattatt taaacaatga aaaagatgag
     5701 tatctctatt ttaatacata cccttatatt cattcattta tcaacaatag ttttaaaaag
     5761 aaagctatct aaaatctatt gtaatctaag tgcttctctt taaagtctta actttagtaa
     5821 tgatatataa gtttataagg ttcttatttc aataatgcaa attttactta gaagttcaga
     5881 ctttggtatg aagaaatggg taataataaa aaagaggtgt tgatatgaaa gaaaaattag
     5941 aagaaaaatc taagattatt aaaacatcaa aagagtataa acgaactata attgaagaaa
     6001 aggacagaga atggaccaaa aaattaaatg acatacttag tagaggtgat ttttctaatg
     6061 aaaaattaga taaaatcaag gatttaattc caaaagaaat attgactagt gaaaatgttg
     6121 gagaagttgt aactgaagat gggtatgcta agcctgagta tgatgaggta tttaaatcat
     6181 tgtttacttt aaataatgat tacgatttac ttgcaaattt tataaatgat atcctcaaag
     6241 atgctccata tgctaatagg aatatcaaac agttcactca aataaaaaga ataattaagg
     6301 ttgaaaccga cccaacaatt aattacattg gtgagaaaag acctagactt gatattttgg
     6361 ctgaagatga agaacgtaac catataaata ttgagatgca aagagctctt gaagaggatt
     6421 atttggaaag agctgaatat tatcttagtc gtgtacatgg tagaaagtta gaagaaggta
     6481 aagaatataa agaaattgga aaaactgttg gaatacatat actaaatcat gtaaaatata
     6541 atcacataga agattatgta aattgcctga gattaacaat ggacgggcat ccagatattt
     6601 attctagtaa aactgcatta tattttatag aacttccaaa aattcgtaaa tccagttgta
     6661 tagcaaacag agttctaata tggggtaaac ttattgataa tccttctcat atagatatta
     6721 gaatactatc taaaacggat tatgttatta aaagagcact tgatagatta aaagaattag
     6781 gtagtaatga ggactattta ttaaatttaa agagaggtgc gtatattatg aataaaagta
     6841 gaaattttaa agaagaaatt tttcaagaag gtattgaaaa aggtattgca aaagggaaaa
     6901 gagaagagaa atttaatatc atcataaaat taaaaaataa aggatacaat ttgagtgaaa
     6961 tatgcgatat tatagatgat ttgaataaat cagaggttga aaaagtctat aatcaaaact
     7021 aatactatga ttgagatggc actagataaa tacaaagctt cctcttttaa atttaaatta
     7081 atataaaact ttgttattta aatatggtag atataaagag atatataaat aataggtaca
     7141 aagaggtgta taaataaagg agataattca aaataaaagt ttgaaccatc tccttcttat
     7201 agaaaaacaa aggggttgat ttatatatga aattttatac tatacagact ttagaatttt
     7261 ggaataacaa taaaaacaat aaatatctat caaatgatta ttcttttata ttagaagact
     7321 ttgatattcc atacaaatgg atgtatagtc aaatgacaaa tagaatatat gaatttgatg
     7381 atagtatgat atgggtttgg acgaagagac ctgatttaag atgtagtggt tatttagaaa
     7441 aaggaaaaaa aggtgtttta cttgaaatag aaataagtga agacaatgtg ttattatcag
     7501 aatttgattt atggcattta cctttaatgg atattactgt agagctttat gaagatgaac
     7561 aaattgaaaa gaaggtaagc tgggaaagaa tatttgactt agaactgtgt tcaaaattaa
     7621 atgaagttaa tatagaagat ttgacattgc aaggagtgac atctaaagtg cctctatcta
     7681 atgtaaaatt aataaaagaa tttgttgcaa aataatattt ttattgaaat ttattcaaaa
     7741 aagttatata tttataacaa gaggtgttat aaatacataa ctttttaagg gggattttat
     7801 atgtctaaac aacaaactgg tattaaatca acacttattt cagaaatatt gggaatttct
     7861 tattgtattg gagctacatt agtaaataag tattttttta aaggaagtag tattgaatat
     7921 cttcctataa ttatttgggt attagctatg ggagctcagt gggtctataa taaaaagtat
     7981 gatgatttaa ttgatgaggc atcaaaatgt atactatcaa gagttaatga tattgcaata
     8041 aaagtattgt ttttttctgt agctatagta tctatatttc ttgtaacttt ggacataaat
     8101 gtgtcaaatt taaatatagg aatgtcttta ttagtcattt tgtttattca atccttgttg
     8161 aagttaattc tatttatata ttttgacaga aaggggatat acaattaggt gtcagtatta
     8221 ctaaagacaa aaattaaatc ctatagagaa aagaacctta tgacacaagg tgaacttgca
     8281 aagtcagtag gagtaagacg tgaaactatt gtacatttag aaaatggtcg ctacaatcct
     8341 tctttaaaac tggcaatgga tattgctaag gtatttgata caactgtaga agagttgttt
     8401 gaatttattg aagaaaataa tatttaaatt tcatgtatta cttatgcatt agagaatgaa
     8461 ttaattatag gtaaaaaacc aagcttatag attagagaat aaatgactta tagatgaaaa
     8521 accaagctca tagattaaag aatgaatgat ttatagatga aaaagaaagc ttatagatta
     8581 gagaataaat gatttatagg taaaaaccaa gctaatagat taaaaaatga atgatttata
     8641 gattaagaag taagctaata gattagagaa taaatgactt ataaataaaa accaagctaa
     8701 tagattagag aataaatgac tcatagatga aaaaccaagc taatggatta aagaatgaat
     8761 gatttataga tgaaaaagaa agcttataga ttagagaata aatgatttat aggtaaaaac
     8821 caagctaata gattaaaaaa tgaatgattt atagattaag aagtaagctt atagattaga
     8881 gaataaatga cttataaata aaaaccaagc taatagatta gagaatgaat taattatagg
     8941 taaaaaccaa gctaatagat tagagaataa atgacttata aataaaaacc aagctaatag
     9001 attagagaat gaattaatta tagataaaaa tcaagctcat agattaaaaa ataaatgatt
     9061 tatagatgaa gaactaaact tatagattaa aaaataaatg acttatttat agatgaaaaa
     9121 ccaaacttat agattaaaaa ataaatgatt tatagatgaa gaactaaact tatagattaa
     9181 aaaatgactt atttatagat gaaaaaccaa actcatagat taaaaaataa atgacttata
     9241 gatgaaaaat caagctcata gattaaagaa taaataactt attagatgaa aaaccaagct
     9301 tatagataag aagtccatgc ttataagcca gaaaatgaat actttaatag attaaaaaat
     9361 acatatttgt gaattataga atgaatgact ccataaacta gaaaatttat aataatatct
     9421 atataaaaat tagaagtatt ttatcacaaa aaaattaagt taaataaata tgaaattact
     9481 tccaaatata cttccaaaaa catgtaatcg catatataat ataattataa aaataataaa
     9541 tatttttata gtagtagtta attattaata aggaggggtc aaaatgccta gcatgattat
     9601 ggaagttgta ggagtactat ttatagctat tggattaatc ttgacattca ttgaaataaa
     9661 aggaaatttc ccttcttttt atggttttgg tcaaaattct atagggaaaa atgctgtagg
     9721 tattatagca gcaggaattg ctttaatttt ctttagaaat ggcatagata gcatagattc
     9781 tatagctact tatggtgtat ttttagcaat agaacttgtt gtaatagttg catatttctt
     9841 aataagaaaa aaatttacta aagaagataa acatttaaga actaattaac taaattatct
     9901 actataaaat tggaaaatta aaaacaaata aaagtttgtt tttaattatt taaaatccaa
     9961 atttgattat ttttaaagaa actatgttct gtataaaact agaatgtggt ttctttttta
    10021 tgtataaaat attttttagc aagagttaaa aggtgtttta taatataaaa tagttaattg
    10081 tagatgttaa taaaagaaaa ttatgtatat ttcagataaa gataagagag tatactatat
    10141 tttaattatt ttttcaataa agcttattga aaagaacttt aacttagaag agtatcacca
    10201 aattgcagat acaatatcat aagcagttat ttattgacaa ccctttttaa taaatatatc
    10261 atttgaagta tactgacgaa aaggagttga ataataatgg acatcttatt ggttgaagat
    10321 gataagaata ttcgagaaat ggtatacgat tttctgatta gtgagaatta ctctgttaaa
    10381 actgccattg atggaaatac agctattgaa atatttaaaa attattcatt tgatattatt
    10441 ttactggact tgatgttacc aaagtctagt ggttttgaag tattaaaata tatacgaagt
    10501 gtgagtgttg ttcctgttat tattactact gcgaaagata gtgacacaga taaaatgcta
    10561 gggcttaatc ttggtgcaga tgattatatt acaaagcctt tttcaatcgt agaattgctt
    10621 gcaagaatta aagctaatat tagacgtgca acagtgtaca gcagttcaag aaactctgaa
    10681 gatatggtta ttcagtataa agatttgatt ataaatatat caaaatatgt agtttcaaag
    10741 aatggaattg acttaaactt aacacataca gaatttgaaa ttgttaaatt actagcgact
    10801 aatcaaggac gtgctttttc aaaggaacaa ttatacaacg ctatttggaa agaaccttat
    10861 tatggaaatg aaaatgtgct aaatactcat attaacaggt taagaaataa attgtctgat
    10921 ggtatagaaa aaaataatta tattaaaact ctttggggaa ttggctacaa aatggaggaa
    10981 gaataatgga gttattttta gttgtaattt cgattgtact atttgctctc tacttaaaag
    11041 aacgaaataa atcaaagaaa atggaaagtg attataagta tatcaattca agacttcgtg
    11101 acataacaaa tgatgatgaa attaattata ttttagtacc atctgatatt tcgattgtaa
    11161 aagaaacagc agtagatata aataaacttc ttgaaaagtt ttacagcagg caaattaatt
    11221 ataatagaag tgaaaaagcg atgttgcaaa tatttacaaa catatcacac gatttacgaa
    11281 ctcccatcac agtattaaag ggttatattg aaatgttata tcttcaaagt caaaaagaag
    11341 aattatcttc ttcaatgaga gcaactgtgg aaaaaatgca aacaaattca aatgagcttg
    11401 ttaattcaat aaataacctg tttaatatgg caaaaatcca atctggagat atgatattaa
    11461 atcttcaaaa atccaatcta acacaattat gtcatgagat tattttagag ttttatgatt
    11521 tgctagataa agaaaacttt aatgttgaag ttaatattga agatagacct atatatgcaa
    11581 atgttgacac agaagcatta caacgtatac tcaaaaattt aattgataat gctataaaat
    11641 atggttcaga tggtagattt ttagggattt cactatatga aaaggaaaaa catgttttta
    11701 tagatattga ggataaaggt tcaggtattt ctgaaactga aaaagagcat attttcacaa
    11761 gagcttatac tgctgacaga aaaaaaggca atggtttagg tttggctatt tctcaagggc
    11821 ttgtaaactc tatgggaggt tcaatttatg taaatagtag acctaatgta aaaacagttt
    11881 ttacagtaaa attgaaaagt tagaaaaaag ttagatttaa aagagagaaa agataatttt
    11941 attttgtata ctgtttttag aaaggaggga gtatgatgaa ctatatcata gagaccaatc
    12001 aattaaagaa aatatataaa gataaagctg ttgttaatgc tgttaatatt catgtgaaaa
    12061 agggagaaat ttatggattt gttggaccaa acggggcagg taaaagtaca gtaatgaaaa
    12121 tgttgctaaa tcttgttaag ccaaattctg gagaaatagt tatgttcggt aagaaagtgg
    12181 cagaaactga ttttgaaata ttaaaaaaga ttggtactat tattgaaaat ccgtatttat
    12241 atgagaattt gactgctaaa cagaatcttg atttacactg tgaatatatg ggttattata
    12301 acaaagaaca tatatcagag gtattggaat gtgtcggatt gtcaaagcaa tcaaacaaga
    12361 aagtttcaaa atattcttta gggatgaaac aacgtcttgc aatagctaga gcaattctta
    12421 ctaaaccaga attattgatt cttgatgaac ctataaatgc actcgaccca gaggggataa
    12481 gagaaatgcg agaattattc cgtaagttaa atacagatta tggaataact atatttattt
    12541 ctagtcatat tctttcagag gtagaacaaa ttgcggacac tattggaatt attcaagatg
    12601 gtaaacttat taaagaaatt tctatgtcag atatacacaa atatcaaaca gattatgttg
    12661 aagtagatgt agataatgtg gagcttgctg gatacttgtt agaaaaagaa tttggaataa
    12721 aagactttaa aattacttcg gaaagttgta ttgaaatata tgatttacga aaagatgtaa
    12781 aagaaatatc caaaatattt attcaaaatc aaattggtat aaatagcatt ggacgtaaac
    12841 aaagttcttt agaagattac ttcttccaaa ctacaggtac aggaggtaaa gaaacactat
    12901 gatacattta atgaaactag agctaaaaaa agttgggcta aaaagatatt ttttattttc
    12961 aatgctgggg attttcttat gtatgttttt tgtctttact ggattgaatg ataaatctac
    13021 ttcaatatat gattatgatg ttacatttag gacaatcagt ttatttttct gtttttacta
    13081 tattatatta tttgctgtgt tgaatgtggc atatataata aatgaatata caaataaaac
    13141 aatcttgatt ttattctctt atccacttga ccgtaaaaaa cttattttat caaagttact
    13201 tttgattacg actcttatga tatcatcaat gatagttgga tatatctgtt gttttacatt
    13261 cgttgttgtg ttggataagt attttaattt agttattgga gattttcata tttcaatttt
    13321 atcttattgg ataccaggag caattaaaag tattattgta ttttgctcat taggtatatg
    13381 gacattcgtt gttggaatga taagaaaatc aatacctgtt acccttgtca gttcaattat
    13441 ttttatcaat ttaagacaat ttattttagc tggtacaaat acaactcaag acagtttgat
    13501 ttctgttttt attatagttg gtataactgt gattggtata tattatatac taaatcataa
    13561 agttactgaa attgactaga tactgtggat aggaaggtag ttattaattg tatcttctcg
    13621 ggataataat gatttgctat tatatcagct attattaata ttattagagt tttaataata
    13681 ttgcacaacc atttgaagtt gagccaaagc ttgatgaatt aattgaatgg tactatagtc
    13741 agagtgaagt atctattaaa gttataactg aatttcatta tagatttgaa ttgatacatc
    13801 catttcaaga tggtaatggt cacataggta gatttgttat gctaaaacag atgttagaaa
    13861 ataatcttcc tataaaaatt gtatcttggg atagtgagga attgtataga aactccttaa
    13921 attcttgtag tttaggtaat tatgtccctt taattgaata tttatcaagt ttagaagatt
    13981 ttagagaagt atataaaatg ttatggaaat aattgtttgc agcaaaaaaa gtttatatat
    14041 aaatgtattt ttatctatgc tatttaacgc taatttctta gttattctta gcttggacta
    14101 tacccactat tttaactata taaaatagtg gatatagttt aactatctct ttataactat
    14161 ttttatgtat cttagttgtt tcttaatatg gggacagttc ataaaactaa aagtgatttt
    14221 taattttatc atttataatt taccattaca tcagaaaaat gcttatcatt agaattttca
    14281 taaaaggtac ctattttttt caattaactg aatcaattct attagtctta atctttcata
    14341 tttcaaattg ataatataag aaaataaaaa ataaaaaatt ttaaattttt tatttattgc
    14401 gaaaaaaata aaaaggtgct atactttttg taacatatta ttcaaattat taagagatag
    14461 taaacattta tagtttaatt tgtagtacac tagcatgatt tgcagaacaa tgaattaatc
    14521 tatagtataa tagtttaatt taaagtacaa cgatttaatc tatagtacaa taacttgatt
    14581 tgaagtacaa cgatttaatc tatagtacaa taacttgatt tgcaatacaa tgaattaatc
    14641 tatggtataa tagtttaatt tgaagtacaa tgaattaatc tatagtataa tagtttaatt
    14701 tgaagtacaa cgatttaatc tatagtataa tagtttaatt tgaagtacaa tggtttaatc
    14761 tatagtataa tagtttaatt tgaagtataa cgatttaatc tatagtacaa taacttgatt
    14821 tgcaatacaa tgaattaatc tatagtataa gagcatgatt tacagtacaa taatttaaat
    14881 tgtagtacaa aagtactatc aaataagaat ttctacttta aaaatattac taaaagtaat
    14941 taaagtccag taattcaata taatatggca aaagaataga aataaggtga gagtagtgaa
    15001 agtaaagtca aatttagatg aaattttata tcataagatt attgaaagtc ttattcgtgg
    15061 tgaatactct gtaggtcaaa aaatattact aaatgacctt tgtgaaaaat ttgaagtaag
    15121 cagaacacca gttgtacaag cagtaaaaat gctaaataaa gatggagtgc ttactattat
    15181 gacaaatggt aaagtgtacg taccagagta tgaatatgat atggtaaaac aagtttgtga
    15241 aacgagaaca ctgattgaaa catatgcttt agaaaaaatg atgcaagaag aggaagaggt
    15301 ttttcaacaa aagctagatg taataaaaag atactctgat aaatgcgagg agtactatcg
    15361 tcaagaaaaa tctgtagaac ttgcactagc agatttggaa ttacataaag ccattgtaga
    15421 aggggcaaat aatgagattt taaatgatgt atatgttggt atacaaggaa gatttatagt
    15481 agtgaactat ttaattcgac cattaaaaaa tcgtaattat gaaggaacag tacaagacca
    15541 ttttgaaata ttaaaattca ttgaagacag agatacaaaa aaagcagtag ataaactaag
    15601 aaatcatatt caaggaacta ttaaaagatt ctgtgaagat gaataataag ttttgaagtt
    15661 actatatata cttatctatt aagttgctaa atgtagtcta agtataatct aattatagtt
    15721 taaacataat ctaaa
//
LOCUS       DAMJZE010000090        15730 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJZE010000090 DAMJZE010000000
VERSION     DAMJZE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.091"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            148..1365
                     /locus_tag="PC541_002347"
     CDS             148..1365
                     /locus_tag="PC541_002347"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2839806.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1460..1840
                     /locus_tag="PC541_002348"
     CDS             1460..1840
                     /locus_tag="PC541_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2839807.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2124..2606
                     /locus_tag="PC541_002349"
     CDS             2124..2606
                     /locus_tag="PC541_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2839808.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2627..3442
                     /locus_tag="PC541_002350"
     CDS             2627..3442
                     /locus_tag="PC541_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2839809.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3493..5043
                     /locus_tag="PC541_002351"
     CDS             3493..5043
                     /locus_tag="PC541_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2839810.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5140..6492
                     /locus_tag="PC541_002352"
     CDS             5140..6492
                     /locus_tag="PC541_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2839811.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6623..7381
                     /locus_tag="PC541_002353"
     CDS             6623..7381
                     /locus_tag="PC541_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839812.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7428..8147
                     /locus_tag="PC541_002354"
     CDS             7428..8147
                     /locus_tag="PC541_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2839813.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8271..9671)
                     /locus_tag="PC541_002355"
     CDS             complement(8271..9671)
                     /locus_tag="PC541_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2839814.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9675..10583)
                     /locus_tag="PC541_002356"
     CDS             complement(9675..10583)
                     /locus_tag="PC541_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2839815.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10844..11491
                     /locus_tag="PC541_002357"
     CDS             10844..11491
                     /locus_tag="PC541_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2839816.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11816..14455
                     /locus_tag="PC541_002358"
     CDS             11816..14455
                     /locus_tag="PC541_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2839817.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14513..15616)
                     /locus_tag="PC541_002359"
     CDS             complement(14513..15616)
                     /locus_tag="PC541_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2839818.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5921 a         1900 c         2747 g         5162 t
ORIGIN      
        1 tattgtatgc aattgttgta tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat
       61 cgtttacatg ttgattttta aaaaaaaaag atatagaatt atatagaaag taataaacaa
      121 aaattggatt tacgggagtg gaatgaaatg atgaatgtta ccttaacaga tgtgaaagag
      181 gctagagaga ccattaagaa catagtaaaa agaactgact tacttgaaag tgttaaactt
      241 agcgaaaaaa caggagcaaa tgtgttctat aaatgtgaga atcttcaaaa aacaggttct
      301 tttaaattaa gaggagcttg taacaaaata gcgagtttaa cagatgaaga aaaggcaaat
      361 ggagttatag cttcaagtgc agggaatcat gctcaaggag ttgcattggg agcaaaaatg
      421 actggtataa aatctacaat agtaatgcca gcaactgcgc ctttagcaaa agttagtgca
      481 actaaaggtt atggagcaga agtagtttta aatggtgcgg tttatgatga tgcatatgca
      541 aaagctgttg aaatacaaaa agaaacagga gcaactttct tacatccatt caatgataag
      601 tatgttatag caggacaagg aactatatct cttgaaattt tcgaacaatt agacaataaa
      661 gttgatacta tactatgccc agttggtgga ggaggaatca tctctggtgt tgctgttgca
      721 gcaaaagcat taaatcctaa tgtaaaaata gtcggtgttc aaactgcaaa tataccttca
      781 atgaaagagt caataaaaaa tggaaaagta acaacagcat ttaatgatac tacaatagct
      841 gatggtatag ctgttaaaac tccaggagat ttaacttttg aaataataaa tgaattggtt
      901 gatgaaatag tagtggttga agagacagaa attgcagaat caatattatt tatgatggaa
      961 agtcaaaaaa tagtttcaga aggtgctggt gctgtatgta cagctgctat attaagtggt
     1021 aagtatgtac cagcaaaaga tgaaaatgta gtttgtataa tatctggtgg taatatagac
     1081 ataaatacgt tatatagaat aataggtgtt gctttagcta aagaaggaag aagatattca
     1141 tttagtacaa ttatggaaga taagccagga aactttgctg agttaactag aataataagt
     1201 gagaatggtg gaaacatttt aagtgctaat caaggtaaat tatcagcagg ggaagctcta
     1261 ggaaaacaaa gtgcggaatt tatattagag acaatagatt atgaccatat agctagaata
     1321 aagaaagcta tagaagaaaa aggttttaaa attatagaat tataataaaa agtgtatata
     1381 atatacaaga aaaatagtat aatagtgtat gaagatacat tttatgtgta ttagattaac
     1441 gaattaggag gattttaaaa tgaaacatga agtaatacat actaatgatg caccagcagc
     1501 tttaggacca tattctcaag ctataaaagc tggaaacttg ttatttgtat ctggacaagt
     1561 tcctcttgta ccagagacta tggaagtagt tgaaggagat gtacaagctc aaactgctca
     1621 atctttaaaa aatcttaaag ctatattagc tgaaagtgga gctgattttt caaatgtagt
     1681 taagactaca gtatttataa aagatatgaa tgaatttggg gctataaacg aagtttatgc
     1741 tgaatatttt ggagaaaata aaccagcaag agcttgtgta gaagttgcta gattaccaaa
     1801 agacgttaaa gttgaaatag aagtaatagc tgtattataa aaatacaata tttttaattt
     1861 gaaaaggctg aaaaactata ttctagtgat tcagcctttt tataaaaaaa taaatcacaa
     1921 taaagaacat gataaatatt gtcgaattaa aaaataaaat taaaataaat attgcaatat
     1981 ttttgagaag gttttataat gagtatataa tttgaaacgt gtaactatta aattaggcat
     2041 gagttcttgg atatttttat cctgaggatt catgcctttt ttgtattgaa attcaaaaat
     2101 ctatttaata aggaggaagt actgtgttaa attttttcaa gaaaaataaa agctataagt
     2161 tacatgcagt agtgagtggt aacagcatta atattgagaa agtaaacgat tctgtgtttt
     2221 caaaaaagtt aatgggagat ggagttgcaa ttattcctaa tagtaatgtt gttgtagccc
     2281 cttgtaatgg taaagtaact gtgcttacag aatcaaagca tgcctttggt atggtctcag
     2341 atgagggtgt tgaaatatta gtccacatag gaattgatac agttagttta cagggtgagg
     2401 ggtttaaaaa tgaagtgtct caaggagata ctgttaaaaa aggtagtcca ataataagtt
     2461 ttgaaagaga aaaaataaat tctcagggaa ttgattgtac aacaataata attgtattaa
     2521 atcatagcga attttcagaa ataaattgca tggtagaaaa tgaagttgtt gcagggcaag
     2581 atactgttat tgaaattatg aaataatagg aagggattaa ttcaatatga gagtagtaaa
     2641 aatctttaat aataatgcat tatctacagt aactaatgat aggaaagaag ctattttact
     2701 aggtcttggt ataggtttta ataaacgtcc aggagataaa gttaatgaag ataaaataga
     2761 aaagatttat tatgttcaag accacatgca aacaaaattt ttagaattat taaaaaatgt
     2821 tagtccagaa gtgatggatg cttcacaaca aattataagt cttataccag gaaatgaggg
     2881 aaaatttaat aataaaggga tattatcttt agtggaacat attagttttg caatagatag
     2941 aatgaaaaat aatgtttttt tacctaattt aatgcttttg gatataaaga tgatgtattc
     3001 aaaagagttt gaattaggag taaaagcttt agaaattata tatagagtat gtcatatcca
     3061 tttgcctgaa gatgaagcag gttacatagc tttacatttt gtcaatttac aatcaaatga
     3121 taatttggca tatgatactt taaaatttgt aaaaggtagt attgatttga ttaaagaatg
     3181 ttatggatta gaattagacg aatcgagttt aagtacatta cgttttagaa ctcatcttaa
     3241 atttcttgct caaagaattt ttaagaatga aatttgtcag gatgataaaa tgattgaaat
     3301 gtatgattat ttaattaaca atcatcctaa aaataaagaa tatttagaaa agttaaatgt
     3361 atatattgaa aaggagttta ggtataaatt agataaacct gaaaaaattt atctactact
     3421 acacctaaca aaaatcttat aagaatgaga tgaattagta atagaaatat ataattttag
     3481 ggggggataa gattgaaaga aaaactactt ggaggactgg aaacatttgc aaaggcaatg
     3541 gttcagccat taatgtattt atcgagtgca ggaatattaa tggtggtagg tgtactactt
     3601 acaaatgata tgttattagg agtacttcca ttttttaaat gggagccaat tcaaatattt
     3661 ggaagcctaa tttataactg tataatggct attattaata atcttgctgt tatatttgct
     3721 ataggtattc ctgctgcact agcaaaaaaa gagaagcaca aagcagcgtt aataggattt
     3781 atgagttata taatatacct tacagcttcc aacactatgt taacatcact aggtcaacta
     3841 gcaaaagaaa gcaagatgct tggactgata gggactggac aagctactat tttaggtatt
     3901 caagttgtag atacaagtgt atttggtgga attattttag ggtgtatagc tggatatgta
     3961 tttaatcgta caagcaacaa aacatttaaa ggagctttac agatatactc gggagcaaat
     4021 tttagtttta tatgcatgtt ctttgtatcc attttaattg gagttgtgag taactttata
     4081 tggccattag ctcaacaagg aatagcttct cttgcaaatg tcatcaaaga tacaggtaca
     4141 tttggtttat tcttatatgg ttttttagag agaatattag taccaactgg acttcatcat
     4201 ttaatctata caccatttca gttttctgat ttaggaggag tgcttaaagt tggtgcagat
     4261 acagtttcag gagcctatgc tatagttatg acagaaatga atttaccagt tgagcgtttc
     4321 tcagatagta tatactacat ggcaactgga tttacaaaga tgtttggtta tataggtata
     4381 ggagctgcct ttatttatac tgcatataag caaaataaga agaaagttaa ggcaacgatt
     4441 attccactaa taattacggc agtattagcc tctgtaacag aaccattaga ctttatgttt
     4501 gtattctgtg caccattttt attctttgta catgcagtta tagctggtct ttttatagct
     4561 ttattaaaaa tatttgatgt tactgcatta tgtggaggga acttattagt atcaattata
     4621 atgaatacta cagtaggcgt agaaaaaaca cactggccag taatgatgat attaggactt
     4681 gcacaaatta ttgtatattt tgtagtattt agttttttaa ttaagaaatt taattataaa
     4741 actccaggtc gtgaagatga gagtacagct acaggcgaag aacaatcaaa acaacttaat
     4801 ttagatgcag ggatagaaaa tataattgat gggttaggcg gaaaagaaaa tattaataca
     4861 gtagaaaatt gcatcactag attacgtgtt aatgtaaaag atgaaagtaa gattaatgaa
     4921 gatataatta atttaacgcc aaatagcggg attgtaagga aaggaaaaga tatacaaata
     4981 atttttggac ttcatgtgca tgaggtaaga cgtgcagtag aagatttttt agaagcttat
     5041 taaagatata tatttatata gcaagggtaa ataacagctt taagtgctgt ttactattta
     5101 ttaaagatac atataattta tagaggagga ataattatta tgattattac tattgttggt
     5161 ggaggaagca catttactcc aggtattgtt aagtcaattg cattaagaga aaaagagtta
     5221 gagcttgagg aaattcgctt atttgacatt gataaagaac gccaagataa agtagctgta
     5281 gttgtcaact ggatactaaa agaagatgtt aaaacagatg ttaaactaac tgtaactaat
     5341 tgtgaaaaag aagcgtatac agatgctaca tttatatttg cacaaatgcg tgttggtaaa
     5401 tatgaaatgc gtgagcagga tgaaaaaatt ccattgaaat atggttgtgt aggtcaagaa
     5461 acttgtggtt gtggagggat ggcatacgga ctgcgtacaa tacaaccaat gataaaggtt
     5521 attgatgatg tagagaaata tgctaaaaaa gattattgga tattaaatta ctctaatcct
     5581 gctgcaatcg tttctgaagc ttgtcgtaag ttaagaccaa atgcaaggat tataaatatc
     5641 tgtgatatgc caatagctat tatagatatg atagcggggt cattaaatat caatgacgtt
     5701 cataatatac gctatgatta ttttggtcta aatcattttg gctggtttac ttcaatagat
     5761 tataaacata gagacttaat ggaagagatt aaggaataca taaaagaaaa taaaatatta
     5821 ttaccaaaat cttatataga aaacttaaat aaaagtcgta gtacaggaaa tcgccatgct
     5881 gtaagtagct ggatacatgt ttgggaatct gtttatacaa tgttagaaag cttcccaaaa
     5941 tatctgccaa acacttattt aacatactat cttttaagta aagaaacagt ggaacattca
     6001 aacataaatc gtactcgtgc aaatgaggtt atggaaagcc gtgaaaaaga cttatttgaa
     6061 ggtgtgagac attacttaga aactggagaa atttctgaaa aagcatttta tgcaggttca
     6121 catggagatt ggattagtga tttggcagtt tcaattaaaa atgatacacg tagtcgtttt
     6181 ttagttataa ctgaaaatcg tggagctata ccaaacatgc catatgatgc aatggtagaa
     6241 attccagcat atattggtaa agatggtcca gaagttattg ctcgaggtcc aatacctcta
     6301 tttcaacaag gattaatgat gcaacaactt aacagtgaaa aattgatagt agaagcttat
     6361 atagaaaata gttatgaaaa agctttacaa gcattcacat taaataaaac tattccaagt
     6421 atgaatgttg ctaaagaaat tttggatgaa atgatagaag ttaataagga atactggcca
     6481 gaattaagat agtaggataa aggaaagggt ttttatttaa taattagata tgttttcaga
     6541 gtcttttatc aaaatgtatt cacaaacata tggcaatata ttaaaataaa gagtgatatt
     6601 tttagaagaa tggggtgatg caatgaacat tataaataaa attttttggg aagaagatag
     6661 gattggcatg attacaaata atttagaggc agatacacat agccactgta tgttacaact
     6721 gttcttaggg atagaagaca gcattgaaat aacagtcaat gaaaaattag ttaaatgtaa
     6781 ttgtatcata gtagataaaa atatctctca ttcattttca gcaagaaaaa aagtttatta
     6841 ctctgctatt attgaaccta catctatata tgcagaacag cttacctcaa aaatgaatga
     6901 ttttggctac tggatatgtg acaatgatgg attagagaaa ttacgtcaac aaggaacttt
     6961 tttgatagat aactctagta aagaacaata tttaagattt atggaaatgc ttaataacta
     7021 tttgaatata cctataactc tcaaacatta tgatgacaga attactgaat tgctcaattt
     7081 attacgtact tgtaattgtg ataatcatac tatttcaagt tttgcagata aagtgtccct
     7141 atcagcaagt agattatcac atctgtttaa agaacaaata gggatacctt taaaaagcta
     7201 tatcttattt catcaaatgg agtgtgcttt tagagaatta ctttctggga aaaatgttac
     7261 cgaagcttct atgactgctg gattcgatac tccatcacac tttgcaggga cagttaagcg
     7321 aatgatggga atgcctgttt ctttatcttt gaaagatagc gaatttttga aagtatatta
     7381 aatattctcg ttatataatg aagttgtact aagaaagggg ctttgttatg aataaagaat
     7441 atttagagga aacaaaaatg ttaaattata atgaaccaca acttaagctt ttggtatcat
     7501 caaagaattg gttggaatta gatgattttc ataaaataaa atctatttat gagtttgtac
     7561 agaatgatat tttatttgga tataatgctt ctgatatgtt aagcgcaaca caggtattaa
     7621 atgatggtat gggacaatgc aatacaaaag ctactctttt aatggcatta ttacgtgcag
     7681 tgaatatccc ttgtaggctt catgcttttg atgtaacaaa agattttcaa cgaggagcta
     7741 catccaaatt gatttcacta ttagcaccaa aatatattct tcatacttgg gtagaggtat
     7801 tttatcaaga taggtggatt gctcttgagg gtgtcataac tgataaaaaa tatttagaag
     7861 caattcagaa gaaatttttt aatcatggtg gaacatttaa aaagtatgca atagctacaa
     7921 atgatttgaa aaatacatct attgattggg atggtaaaga tacttttatc caaaaagaag
     7981 ctatcgtgta tgattatggt atttttccat caccagacgt atttttttct actcattcac
     8041 aacatatgtc aaaattaaaa aatttcatat atgtacatct tatcagaaaa ataatgacaa
     8101 aaaatgtatg taaggctaga aacaattata ttgataaaaa cgaatgagtg tttaagaggt
     8161 aactatatag gaaatatata gaataattaa aactgtactg gttaaaatga ataaaaagga
     8221 gttatcacag agcaaactta aattgttcat aggataactc cttttctgta ttaacttcta
     8281 ataatttgat gtctttttat aaaacatata gatacaatta aaccagtaaa tgcaagagta
     8341 gctgctattg aaaatgcaac atctattcca tgaatgttag acagcatgtt tgaactcata
     8401 tgagctttct tagtagcact tgtcattata gtaataagta tagcagaacc tatagctcca
     8461 gatatctgac ggagtgtgtt attaattgct gtggcatgag atataagttc tctatctaat
     8521 gttttaattc cccatgtagt taatggcatt aaaaccattg aaataccaat catacgaata
     8581 caatacatca atgacacatg aattaaagat gtatctctgc ctaaaaaagc gaaggataaa
     8641 gttcctaaaa acaaacatcc tgttcctaat attgatagag catgaggtcc atatttatct
     8701 aaatgacgtc ctgcaattgg attgagtaca accatcaata tagcaccagg taacattaat
     8761 gaacctgaag ataatgcaga aaatcctctt acagattgaa cgtacagagg aatcattaat
     8821 gtagcagaca tcattgaggc atatacaatc ataattaata ttgtagaaat aagaaatggt
     8881 ttatttttaa aaaccctcaa ttctaaaaaa ggttctttag attttaattg acgcaatgta
     8941 aataaaatta atgacataat tcctattaat attggtaaat atgtggcaat attagtccac
     9001 ccataattac cttgattagt aaatccaatt aataaaccac caaaacctag agatgatagt
     9061 actattgaag gtatatctaa ttttagtttt tgagtttccc caacatttct taatagaata
     9121 aatgatagta taacatctaa tatagcaata ggggaaagaa acagaaataa actatgccaa
     9181 ccaaatgaat ctactaacca tcctgataaa gttggaccta ttgctggagc aaatcctact
     9241 gtaataccaa ctaaactcat agctgcacca cgtttttcct cagggcataa atagagtata
     9301 ataacttgta aaagtggcat tagagaacca gaacccattg cctgcaacac acgagcaatc
     9361 aacatagttg aaaaattatt agaaaaaagt gcaataatac atcctataga aaagatagac
     9421 attgatgtaa taaataactt tctagtacta aatcgattta ttaaataagc tgttgtgggt
     9481 acaataatac ctgcaataag tagataaatt gttgttaacc actgaccaat atcagcagta
     9541 attttaaaat ctgacattat attaggtaat gctgttgaca atagtgtttg actgagagaa
     9601 cttataaaag caccagtcat tacaattgct attattaaat ttcctttttt attgttactt
     9661 tgggtttcca taatttataa cgtctccttt tttaattgat ttttaatgct gttcaataaa
     9721 agtacttttg gtcttaaagt gtcttctttt tctaaatatg caatcattgc atccataatt
     9781 aaagaatcat attgttgatt ttttccttcg acgtataatt tttttattgc ttctagtaat
     9841 ttaggatttt caaggagaga agtatcagtt ttgattagtt cttctatatc ttgtatcgtt
     9901 ttaaaaaata gcgatttatt atgaagggaa aagtaaactt catcttcttg tttatgtaaa
     9961 aacattgatt tttcaattaa aggatttaga ttatccatat tttctacaac taaatctaga
    10021 atttgtatag taaatttggc aataaagtat acatttattt ctcttttttg tacatgaata
    10081 atgtatataa actgatgaat tagagcatcc aataagaaaa ctaaatcaaa tataattttt
    10141 tctattttat caccatagtt atcaaataag cttagtttgt attcattcaa taagttagct
    10201 cttgattttg tttgcaattt acttgtagcc ttacgttgct cttcagaaaa gttttctctt
    10261 atataggtat ttatggtagt tctagaaaaa atatttttcc atacagcaat tatctttctg
    10321 tttaactttt ctgatgatgg aatgtcatta ttttcattta tggaatgtat aatttctaaa
    10381 aacttttcat cataataatt gattatttcg cagataaaac tttccttagt ttcaaaatgc
    10441 ttgtaaaaag tagctttaga cattttacac tcttttacaa catccgatat tgaagtattt
    10501 tgaaaacctt tttggtcaaa aattttttgt gcaatagtta taatgagcct ttgtttttct
    10561 gtcactttat tatctccttt cacgcaaaaa cgtttagtac tatatagtac ctacaagttt
    10621 tgataaatat attatacatc atttggaaaa aaaatcaata tatagactgt aatatgaaat
    10681 ttttgaaaaa tttgttgtta tttttagtcg tataaaaaag aaacttaaga gtttcttaag
    10741 aattgtttaa agaagttgca ataaattagt ggtatgatta tagtatgaaa gagaatgtct
    10801 tgaaattttt ctgtatcaaa ttccaattat ggaggtgtag tatatgaaaa agaaaatttg
    10861 tgtagcaata atttttgctc tatttatagt ttttgtagaa atgtttattg atagctctag
    10921 cgaaggaagt aaaattatga cagctgaatt aagtaaatac aggaaaggcg taaatgtatt
    10981 atcaataaac aatattatag tagcgaataa aaaatatagt ttgcccaaag attattctcc
    11041 tcaggaaagc agtgaagcga gagatgcttt ttataaaatg aataaagatg ctcaaaagtc
    11101 aggattaaat ttaaaagctt ttagtacata tagaagttat gaatatcaag atagattatt
    11161 taagtcatat gtaaaagaac atggagaaaa agaagcaaat agattttcag ctaaacctgg
    11221 agagagtgag catcaaactg gacttgcttt tgatattgga ggagatgacc agtcttgttg
    11281 ggcaaataaa aagttcaaca atacaaaaga agctaagtgg ttgtatgaaa atgcatataa
    11341 atatggtttt atacttagat atccagaagg taaagagaat ataacaggat atatgtatga
    11401 atcttggcac tatagatatg ttggaactga acatagcaaa aactttgcaa tgaataattt
    11461 gactttagaa gaatatttac atataaatta agagatggtt tgttaaaatt aggttaaaat
    11521 aaaaaataac ctagactatt agctgtgtaa agtgatataa tgtaaataca atttaagata
    11581 actactcgaa ggggagtagc ttacacgaaa gtggtaatta atcgtcaaga cagtagaaat
    11641 actcggttaa ttagcaaggg attccttgat agcaagacct tcaaaataag cattaggtat
    11701 gtttgttttg gggtcttttt ttattcaagg aatttttatt taaaaaaatt taaatttcaa
    11761 gtatacataa gagcccccaa acataaaata aatttattga agaggagggc aacaaatgtc
    11821 ttacaaaagt acttatcaag aagtaattaa aaatttagac tcaagtttat ctggtttgag
    11881 taaagaaaga gcagaagagt tgttagaaaa aaatggagct aatgaattaa aagaagcaga
    11941 taaggttcct acttataagc ttttcctaga aagttttaaa gacccattag taataattct
    12001 attgatagct gctttagtac aaatattttt aggtgaaaca gtggagtcta taataatttt
    12061 tgcagttatt attataaact ctgttctggg tgttgtacaa acaaaaaaag cagaaagttc
    12121 attagaaagt ttgaaaaatt tatctgcacc aaacgcaaag gtaataagag acaataaaaa
    12181 aatgacaatt ccagcaaaaa atctagtagt tggagatatt gtattcttag aagctggcga
    12241 ttacatacca gcagatggaa ggcttataga agcacaatct ctcaaggttg tagaagggat
    12301 gttaactgga gaatcagaac cagttttaaa gcatatagaa aaaataagtg aagatgtaag
    12361 tttaggtgac cagaaaaata tggtatttag tggtgctgta gttgtttatg gtagaggttc
    12421 ttttgtagtg actggaacag gtatgaatac tgagatgggt aaaatagctg ggcttttaga
    12481 aagtgctgaa aataaactta caccattgca acaaaaaata gaggatttta gtaaaaaact
    12541 tggtgtaggt attgtaatac ttgctctttt aatatttata attcaagtag caagaaatta
    12601 ctttatggct ggaaatggag aaatgacaca aattatttta gattcattca tgttctctgt
    12661 agcagtagca gtagcagcta taccagaagc tctatcatca atagtcacta tagtattggc
    12721 tgttggaaca aatagtatgg caaaaaaaca agccataata agaaaattac cagcagtaga
    12781 aactttaggt tcaacaagtg taatatgtac tgataaaaca ggtactctta cacaaaataa
    12841 aatgacagta gttgattatt atatgtatgg aactaataat gatgagttag ataaagaaaa
    12901 agtaaactat tcttatcatg ctaaattatt gactatggtt tctacattgt gtaatgactc
    12961 tcatataact agtgatggta aagaagttgg tgacccaaca gaagtggctt taataaatta
    13021 ttcaagtaaa aatgatttag attatgataa attaagaagt aagtatatca gaataaatga
    13081 aataccattt gactcagaca gaaagcttat gtctactgta aatagtatat atggagatta
    13141 tataatgttt acaaaaggtg ctccagatat tgtgtttagt agatgtaaat atgctttgaa
    13201 aaatggttca aaagtagata taacagatga gataatagaa gaatataaac ataaaaatga
    13261 agagttctca aatagagcac ttagagtatt agcttttgca ataaaagatg tcccagaaga
    13321 agattttata ccttcaattg atgacgaaca tgacatgaca ttggttggaa taatggcaat
    13381 gatagaccca ccaagagaag aagtaatgga tgcagtaaaa gaggcaaaga gtgctggtat
    13441 aaaaactgtt atgataactg gtgaccataa aacaacagca gcagcaattg caaaagagat
    13501 tggtataatg gaagaaggag acttggctct tactggaaaa gagcttgatg ctttgagtga
    13561 agaacaacta tatgaaaaac tagaaaatat aagtgtatat gccagagtct cgccggaaaa
    13621 taaaattcgt atagtaaaag catggcaaca taagaataat ataacagcaa tgactggtga
    13681 tggtgttaat gatgcacctg cattaaaaca agcagatata ggaatcggta tgggaagtgg
    13741 tactgatgta gcaaaagatg cttctgctat ggtattggtt gatgataact ttgcaagtat
    13801 agtaaatgct gttgaagttg gtagaacagt atacagtaat ataataaaat caattactta
    13861 tctatttgca ggaaacttag gagctatagt atccatagta tttgcagtgt ttgcaggttg
    13921 gtcaaatcca tttacagctt tacaactttt gtttataaac ttagtaaatg actctttacc
    13981 agcaattgca ttagggtttg aaaagtcaga aaaaaatata atgaataaac ctcctagaca
    14041 cccggatgaa agtatactag caggaggaac attgaaggca gttgctataa gaggttgtat
    14101 aataggtgta gttacaataa ttgcacaata tataggatta caagtatctc cagaacttgg
    14161 agtagcaatg gcattttcaa cattgatatt agctagaata atgcaaacat tacctgcacg
    14221 ttctaataat caaacagtaa taaaattagg ttttttcagc aataaatacg ttataggagc
    14281 agttttagtc tgtcttgggc tttactcttt aacactaata ccagtgctaa gaggaatatt
    14341 ctctatacca acaacgtttg gattgtacca gttggggatt tgcctagggt tagcattaat
    14401 ttcatcaata ttaatggaac ttaacaaatt aataaataga agtgaagtaa aataatcaaa
    14461 aaattaaagg gcatattgat tttaacaata tgccttttat aaatagtcta atttattcta
    14521 tattatcgat tatttggaat gcttttataa cttcaggtaa ttctttttct tcaggcattc
    14581 taaatcttat aaaattttta tcaagtccag taaaatcact tccagatata actcttattt
    14641 ttaatttagc aaactcttgt tgtaaatcta tgtccttgtt tttatgttca actgtcataa
    14701 ttgatacagt atcactagtt tcagctatat taaggttttt ccatggaatt aataattgat
    14761 tttttatatc ttttgttttt tcttttaatt cttctataaa ttgaacatca tctaataaat
    14821 ttgctgcaat actacgagat atttcagaca cttcgtaagg agtagaaatc tttttaatag
    14881 ggctaacaag ttgttctgga agaactgcat atcctgctct tagtcctgct aaaccaaatc
    14941 cttttgaaaa tgtttttaaa gcaattacat tatcataatt atttagaagc ttgacagctg
    15001 aattttcttt tggcatgtat tcaccataag cttcatctac cataactgct atatcatatt
    15061 tagctgcttc ccttactata ttttcaattg atgataaagg tataatttgt cctgtaggat
    15121 tgtttggatt gtctatgtaa attactttat attctggatt tattttttca ataaattctt
    15181 tttcattaaa cttgaaatta tcctctttct ttaaaagaac ataatcataa gttgcaccat
    15241 gcatttttat atctgtctca tattctgaga actgaggtga atatcctaaa actttatctc
    15301 ccttttcaat aaatagtcta tttaaaaggt aaataacatg tatagaacca tctgccaaac
    15361 aaattctgtc agtatctaat gctataaaat ctttccaata atcgactata ttatctttta
    15421 aagcaattga atgaggatat cctcttatca tatcaaattt taaagcatta aaagcttcta
    15481 cagctttgtt tgagtaagac acggtattta taccttcacc acagtctatc tcgaatacat
    15541 cttctgaatg tccagattct attgcataac tttttttcaa ctgcatttca atagctttgt
    15601 taatttttag tttcatactt tatcactcct tatttagtca ataataaatg ttataaaagt
    15661 tgctagattt aaaatattat gaaaatatat aatagtaaaa atgaatataa atattataat
    15721 agctcacttt
//
LOCUS       DAMJZE010000091        15718 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJZE010000091 DAMJZE010000000
VERSION     DAMJZE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.092"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            319..2553
                     /locus_tag="PC541_002360"
     CDS             319..2553
                     /locus_tag="PC541_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2839819.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2584..3429
                     /locus_tag="PC541_002361"
     CDS             2584..3429
                     /locus_tag="PC541_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2839820.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3669..5402
                     /locus_tag="PC541_002362"
     CDS             3669..5402
                     /locus_tag="PC541_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2839821.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5734..6214
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5800..5822
                     /rpt_unit_seq="ataccacttagttaatataaaac"
     gene            complement(6744..6848)
                     /locus_tag="PC541_002363"
     CDS             complement(6744..6848)
                     /locus_tag="PC541_002363"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2839822.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7473..7718
                     /locus_tag="PC541_002364"
     CDS             7473..7718
                     /locus_tag="PC541_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839823.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8471..8617)
                     /locus_tag="PC541_002365"
     CDS             complement(8471..8617)
                     /locus_tag="PC541_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839824.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8650..9036
                     /locus_tag="PC541_002366"
     CDS             8650..9036
                     /locus_tag="PC541_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839825.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENI
                     CDFVTTPIKKVVQLLSFIQNKKLNYY"
     gene            9903..11258
                     /locus_tag="PC541_002367"
     CDS             9903..11258
                     /locus_tag="PC541_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2839826.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11376..12044)
                     /locus_tag="PC541_002368"
     CDS             complement(11376..12044)
                     /locus_tag="PC541_002368"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2839827.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12111..12539)
                     /locus_tag="PC541_002369"
     CDS             complement(12111..12539)
                     /locus_tag="PC541_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2839828.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12646..13239
                     /locus_tag="PC541_002370"
     CDS             12646..13239
                     /locus_tag="PC541_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2839829.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13358..14293
                     /locus_tag="PC541_002371"
     CDS             13358..14293
                     /locus_tag="PC541_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2839830.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14564..15508)
                     /locus_tag="PC541_002372"
     CDS             complement(14564..15508)
                     /locus_tag="PC541_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2839831.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         5969 a         2069 c         1972 g         5708 t
ORIGIN      
        1 aattaattat aaaaaaactc taccttacta actatacagc tcacaatcta tctatttatg
       61 aaaaaatatg atataattgc attcaaaaag gaaatctttc aatatactag aatttaagca
      121 ttaactaatt ttatctaata ttattactaa actaaaaaat atttgtattt gtagcattgg
      181 agcaaataac ttaatgtata ttcattttaa atacttattt aaaatttagc atttaaatta
      241 tttacaaatt aacatttaaa tacttctagc ataaatacag ttgtcatcaa aatttaaagt
      301 atcttatgga ggaataccat gcaaaatcat aattatataa aaatgttaaa taaaaaggta
      361 gaagtattat ctaaggaatt agatagtaga aattttaaat taaaaacctt aaatgaaata
      421 gatttttttt ctaaaatacc aaacagagaa tttatgtacc aacatattga tgactgtatt
      481 tcgaaaaata tacatatagg aatcatgtta ttaaatatcg ataactttaa attatttaat
      541 gatatatatg gatacctagt cggagaaaat ttattaatta ctctaactca aaacctattg
      601 cactttattc cagaatcaaa gggaatagtc gcttatttaa aaggagaagg ttttttaatt
      661 gtacttccaa atgtatcaaa taaagagtta aataatatgg gtgcaactat tatagagagc
      721 gcccgagaat taaaaataag acttcctaat gaaaaagaaa tatactccaa tgttacattt
      781 agtatcggca gcactatctg gaatggtgaa ccaaacatga ctactttgat gcttttcaac
      841 caagtgtata gtgctttaaa aaaggctaaa agtgaaggta aaaatagata cattctattt
      901 gattttaaag ataatagttt ttacaataaa ttagaaaatg ataaggagtt cttatcagaa
      961 atatcacatg ttctacaaac taaacaaatt gaagtatttt accaaccact ttataatata
     1021 aaaaataata ctatagttgg atgtgaggca cttgcaagat ggagacaccc tattaaagga
     1081 cttcttactc cagataaatt tattccttta tttgaacagt ttggtctgat aacggaatta
     1141 gatttgcatt tatttgaaga gaactgtaaa aatataagaa gatggattga taacaatgat
     1201 gtttttgttc caatttgttg taacttttct cgtcttcatt tttttaaccc taattttcca
     1261 agtatgctaa aaggtattac tgaaaagtac agcgtctcta catcaaattt tagcattgaa
     1321 ataacagaaa atatattaat agaaaataca aatacaatta ttcaacaact agctgaactt
     1381 cgttcttatg gtttttctgt atcaatggac gattttggtt ctggatattc atcttttggt
     1441 atgttgcaaa atctaccttt tgatataatc aaaattgata ggatattttt atctcataat
     1501 ttaaatgatt ttaaaaatac aaccattttg tactctataa taaacattgc aagagctctt
     1561 gggatgctta cagtatgtga aggtgttgaa acagaacaac atgtagaatt tattaaaagt
     1621 ataaattgtg atatcgccca aggttattat tatgcaaaac ccatggagtg caaagaattt
     1681 gaaaaattac tatctaaaga gaataattca gagaaaatgt ttataaaata ttctgatata
     1741 ggaaaatctt taatagagaa gatatttgat ggatttttta ttatgcaaga tatgaaaaaa
     1801 ctaagagcta atgtcactga agacattcaa tggtttgatt tttacaacaa aactgaaatt
     1861 acatcttttc ctaaagtaat agagcatttt gaaaaacata ttaaaggtaa aaaattttct
     1921 attctttaca aaagtataac cccacataat actgaatcag attcattatt aattagtgga
     1981 gaagccgttt tatcagatga agcttctgaa catcaaaaat atagcaattt ctactttagt
     2041 gcaaattgta taatttcaga aaataaatta ctcttagcaa aattacattt ggatttaatt
     2101 aaaactgctg gctatataaa tgaatttgaa tcatattcaa tacctaaaat gacaaataca
     2161 catcttgatg aaagtaatat tttatcacaa ttttattcaa tgttgcctgt aggtataatt
     2221 cgctacgatt tatttggtga tatgattatt acttatatga atgaagaaat gtttgatatt
     2281 ataggttata caaaagaaca gttttttggt gagatgggcg gaaatttaag agcaattgtt
     2341 catccagatg atttagactt tgtttataaa aaaagtttgg acatgattga cacaaataaa
     2401 attgaaccat ttttatatag attcataaaa cgagatggtc aaattgtcac agttatgtat
     2461 acacaatgta acctttctgc aattgatgga aggtcgattg cccagggtat gtatgtagat
     2521 gtaaataaaa taaaaaattt aacaaaaatc tagtacaaag tattttattt tggagaaatt
     2581 aatatgttta aagaaatttt tttaagaact tttccaggat ttctaattat aagagattct
     2641 aactacagaa ttatatttat aaatgataat ttaaaaaatt taattaaaag ttatatgcaa
     2701 gataatccac ttggaatgac taatatagaa atagctaaga aactccctga taatattgct
     2761 aaatttttta cggattctca caatattcaa ttagattggg aaaaaaatta tccttatgat
     2821 aaaatttcaa attggatttt agagtttaaa aaagatacta caagctattg gaatgtttta
     2881 gaatacaaag tagatgttga tgaaaaaacc tacataatta ctatggccaa tgatataaca
     2941 aaactctatg aagaaaataa gagaaatctt cactactcaa ttactgaccc attaactgga
     3001 gcatataata gaaaatattt aaatgataga tttgacatat ttataggaga ttatattgta
     3061 ctaattgact tagataattt caaaatgata aatgactatg aaggtcataa tgtaggagac
     3121 aagattttat gtgattttgt ttcactttta aataaagaat taataaattc tacctctatt
     3181 atacgattag gaggagatga atttattgtt atattctcta gtgatgttga caaaaattat
     3241 gtttattcac agctagaagc cttaagagaa aattttttaa aagtattctc taagtataag
     3301 tatctttcat ttagttatgg tgttgataca gtaaaaagaa atttaaaatt gacaatagtt
     3361 gaattggata aaaaaatgta taaaaacaag gagaaaaaca agaaaaaatt tgataaaaat
     3421 gattattaac attgttattt taaaataaat taaaattatc tttctttttt taaacttgta
     3481 cattagttta taaatatgta tctatacaag atattgacat taagtactga agcatatatt
     3541 gtttcagttc ttttttgtta aaattaactt atttaacaaa gatatcatag tttaacaaag
     3601 cttttaattt aaatatattg atgtataata taattaaaat ataatagcaa aaagaaagga
     3661 ggaatatcat gagcatactc ctaaaaaaag cacccaaatt agcaaaacat attataacaa
     3721 gtttttatat aaatcgagat atcaatgaag tattaaaata cctttgtgaa aatgttacat
     3781 ggattggacc aggagaacag gaatttctca cttcttttaa tgaaataaaa aattattttt
     3841 atgcaggtca agatgaaatt ccttcttgtg atataaataa tgatatattt gaaactgtaa
     3901 gtgaagatga gaatagatgt atggtattag gaagatatac tgttcgaact aaagaaaatg
     3961 ctcaaatgat tttagaggta aatcaacgtt gtacatttga gataattgag gatagaggaa
     4021 aattattaat aaagcacatg cacatatcaa acccttatgg tgaaatgcaa cttgatgaat
     4081 actttcccac taaaattgga actcaaagtt atgattattt gcaaagatta ctaaaagaaa
     4141 aaacagaagt tattgaaatg attacaaata atatcaatgg tggcttaaaa ggtagcaatg
     4201 atgatagtac atactcattt ttctatgtca atgagggatt gcccaaaata ttaggataca
     4261 catatgatga gttcatggaa atgagtggtg gttcagcagt gggtgctgta tatccaccag
     4321 acttacctaa agcactagaa gattgtgaac gatgttttgc aaaaggacct acatattcat
     4381 ctgaatatag aatccgaaaa aaagatggaa cattaatgtg ggtattagat tctggaatga
     4441 aatcgttaaa tagtgatggc atagtaaaaa ttaatagtat tattactgac ataacccaac
     4501 taaaaaacat agaatctgaa ctaaaattag aaagagagcg atatcgtatt gctttgcaaa
     4561 atattacaga tatcatgttt gagtatgata ttgaaaacga tacccttata aaatatcaac
     4621 gtgtagaaat tgacaaaaaa attgaattag aaaattttga aacaaaaaat tactccaaac
     4681 tattagaatc tggtaaaatt attcaccttg atgatattgg aaaactacta gaagtacttc
     4741 atggaaatct tcgtgaaaca attgagataa gacaagtaaa ttcattaact aaaaatgaat
     4801 ggagatggat tcgtgttcaa tgttcagtta tatatgatag tgaccataac cctatcaaaa
     4861 caataggcgt tttaaaagac ataactgaag ataaatctaa attagaaagt ttaattaatc
     4921 aggctcaacg tgacccacta acacaattat ataatcaaag agtatcacaa aatcttattg
     4981 aagagtatct atgtagttct gattccaaaa ataatgatgc tctattagtt attgatattg
     5041 atgattttaa aactgtaaat gatacttttg ggcatctaga gggaaatgaa gttttagttg
     5101 ctgtatcaaa aatactatta cataacacac atgataaaga catagttgct cgaattggtg
     5161 gagatgaatt tacaatattt ataaaatcat taacaaagga tttaataata aaaattacta
     5221 atgatatttt aaatgatgcg agtaaaataa aagtaaagga caatcataaa ataacattaa
     5281 gtattggtat tgccttcact gatgattcaa ctaaattata taaagattta ttttctaaag
     5341 ctgataaagc tctttatcta tcaaaagcag atggcaaaaa ttgttatagt acatacgaat
     5401 aataaagcta tttatacgac caagaatgaa cctttaaatt taatttcata gtctaacaat
     5461 caaagagttg aagctaatct tatagctagt caactagttc ctagaaaaaa tttaaacaat
     5521 atatttattg atactgctca tttttctaaa gagcaaattt atataaaaaa ttaaactttt
     5581 atatacatac agatactaat gataaacagt tatcattagt atcttctatc ttaaaacaaa
     5641 atgataattt atctatataa aaaagtgctt tgttgacaga gttcactttt ttatataggt
     5701 acagaattta agtataaata catgttttat tctatacatt ttagttaatg taaaatttct
     5761 tgattgtact ctctaacttt tcaacattat actatttata taccacttag ttaatataaa
     5821 actataaaag tgttgcaatt actgggtttt ataaagttaa tttacatacc acttagttaa
     5881 tataaaactt tagcacctta tttatctata gagctacaga aagtatttac ataccactta
     5941 gttaatataa aacatgtatt tatttgttct tgtactattg ttttactcta tttacatacc
     6001 acttagttaa tataaaacaa tatcaaaaca aataaaaatt tttaataaat cattatttac
     6061 ataccactta gctaatataa aacattctgg tactgttgct atttatgttc cgtattcaga
     6121 atttacatac cacttagtta atataaaact ttggagttgt tacagattat ttgccttatc
     6181 tctctattta cataccactt agttaatata aaacccccaa ataaactggg cattttcaat
     6241 acttacacat atgcaactct cttaaatttg cagtgaacca tgagtagtgc aaatgataaa
     6301 gcttatcata taccctcaac accttatatt tcaagcgtta agctctactt tgtaacaaat
     6361 atcgctcact gcaaaactat ttacattttt attatatcat aaatatatta tttttgaata
     6421 tctataccaa tttgtggtat aataaaagca agaagaacta caatctattt agacgtagag
     6481 tgaagttcaa atactaatgt ttattctttt tgaacttgat ttttagttca aaactaaagt
     6541 cactcctgcc tgtgtgaact atacccgctg ttttatacag ttaaaacatg taaaaatagt
     6601 tataaagtac tagtgtaact cattagtact ctttttatta tattcatata attaatatct
     6661 atattttaaa gcttactatc taaaaaagag cacgtcaaat aaaaccctag atatttaatt
     6721 tttttagaga tacttaaaat aaattatgct ctctctgtac ttaaatattt cctaaattca
     6781 aatggagaca tagaatttaa tctattttga tatctaaaat tattatagta gtatatataa
     6841 tcatctattg ctttaactaa ttattcatat gtatcaaatt tccttaaata gtacatttct
     6901 acctttaaaa ttccccaaaa tccttccata ggagcattgt caaggcaacg accaactctt
     6961 gtataatata tgtatatcta acaccattaa gaaatatata ttttataata tatatttctt
     7021 catttaatgc acttagaaaa gcaaagaaaa tacagattag tacacttttt agagaaaaat
     7081 aaaattaata catctaaaat attaaaaaat actttagata atctaaagac acatctcaaa
     7141 gtttttaact aaattataaa actaattaca aatgctatgt aacgataata aataaaaaat
     7201 ttacttcgct taagcgacgt gtcgttgaaa tatttcatgt tgccaactga ttcataagtc
     7261 gataagaaaa gaacactaat tatttagagt tcttttctta gctacaagtg gattataata
     7321 cttttaaatc tgtgagaaaa tcatatataa taatgatatg aacaagtaaa gaaaatacaa
     7381 aaatgacaaa attataaata aaatattata aaatcataag gtcataattg ttatattgta
     7441 gaaacaaagt aaagagagag ggtggagaat aaatgaatca aaaaaaaata aagattagta
     7501 ttaaaatggt aattataggt atattttctg taattattgc ttttgtatta tccagattat
     7561 tcgttgagta tattgatgaa ttattaaggt ctaattggca atatcagcta tttgaaagtg
     7621 agagttcata tataatattt gccaattgta ttatgttctc gaatatatta ttagaaatat
     7681 ttttaatata tatttgtaga aaatttagaa aaatctaata tggtcaacgt atttcgaatc
     7741 gaatacttca tgattattta gtctattgtg ctagttaaag tttaattccc atataaagta
     7801 aattgatttt agtaaaacaa atcaccccta agggttagtt tatggataag tcatccaaat
     7861 attaactctt taatttttgc tatagattca tctttgctca taactttact tataaaaaat
     7921 gaaatattat aagctagaac ttttattcta attctagtca taaatcctag catagagtta
     7981 ctttttttat attataaata aaactataca acaaagataa tatttagtaa aaataacttt
     8041 cattttaact aaacttaaaa ttcaattact ctctaaattg acgtttagga attagttcaa
     8101 aataaaaaag agtgtatata ttgaaattac tacatttcaa tatatatact ctttttaaat
     8161 ttctcagttt tccatggaac tttactttat ttatttctgt attagttgta ttttttatgc
     8221 atttttcttt aaaatcatat tttctgattc aactcttata ttataaaatt tctttactaa
     8281 attattctct ttttctatat cttcatatat attttttaat cgccttttac ctaatctttt
     8341 atctaacatt actaatgatt taactaatat attatatgat tttatcgcat cttctattga
     8401 gatatttaaa catatattta ctgcttcata aaaatcaaat tcattaaaaa atccattttt
     8461 atttacaatc ttatatccct ttgcatctct ttctaaatac tcttcctaaa tactcttcaa
     8521 aactttcatc atctaatcta aatcctatat cactatgaaa aataggtttt gcatttggat
     8581 atagttctat tgttttatca aaagtttcga ataccaagtt attattattc gattttccaa
     8641 tagtccacta taaacaataa taagtttgtt gctccaaata atgtaacact tgtagaaaga
     8701 tgttacagat atataaaaga tagagaatgt agtttttctc ctaatactat tagaaataga
     8761 aaaaatatga ttaaaaatca aattgaagtt ttctttaaag atatgaaatt aatagatata
     8821 acaccttcta ttttacagtc ttatataaat aacatataca atgagcacat gttaaattca
     8881 ataaaaaacc aagtcgattt tataaaatca gtattgaaag aatcttatag attaaaagaa
     8941 atatcagaaa atatttgtga ttttgttaca accccaatca aaaaagtagt tcaacttcta
     9001 agctttatac aaaacaaaaa gctcaattat tattagaaaa atcaaaaaat attcctatag
     9061 gtataccaat ttttttgatg ctaactcttg gattacgttt tggtgaagct gtatcactta
     9121 tatggtcaga tgtagacctt gataaaaaat ctcacatgta aatcaaacat tagtttatgt
     9181 atacaataaa ataatcttca aagaccctaa aacccctata tcaaaaagaa aattatttgc
     9241 tcctgatgaa cttataagtt tgcttaaagg ggaaaaatta aaacaaaata agttaaaatt
     9301 acagggtata ttgaaaaatg aatttgattt gatatgctta aataaaagat tcaacccgtg
     9361 agttcaacca acttttgttg ttcattttaa aaaactatta agagataata atcctagaga
     9421 tataaaatta catgaattaa tacatgcaaa tgcaactttg atattacttt caggtactaa
     9481 cataaaagct atttataaaa tattagtaca tgcagatata aaaatagcta tgaatatata
     9541 ttccatattc tcacttatta gatgaaatga ataaagaaac atctgaaaat ataagcaaaa
     9601 tactattcaa ataatgttca gttttatgtc aattaaatgt cactcaaagt gctttttttt
     9661 catttgtcag ttaaatgtca cttaaagtac ctttttccta tttgtcagtt aaatgtcagt
     9721 aatatataaa taagatatat ttataagacc cttaaaatca ctattattta ataaaattta
     9781 taattatata ctatttgctg ttgacattgg tattatacca tagtttatca tttatattag
     9841 aaataaaatt attaattaat agtttcgtaa ttttataata gatttacagg gagtgatagt
     9901 aaatggatac aaaaatatct ataaacgaaa acatgagtct aggtaaaaga ttttttaaat
     9961 acctagcacc atctgtagtt gcaatgtggg ttttctcact atatacaatg gttgatggaa
    10021 tatttgtaag taaaggagtt ggagaattag ctttagctgc tgtcaatata tcaatgcctt
    10081 ttattaattt tatatttgca gtttctctat tattttcaac tggtgcatct accataattg
    10141 caatctatct ggggaaaaaa gatattaaat ctgctaacga agtcttttca tttaacttag
    10201 tatcaataat tatattatca ataattatat tagctataac tttttttaat ttagatagat
    10261 tagcactatt tttaggagct acagaatcta caattggtat ggttaaagat tatttaggaa
    10321 taataatatt ttttaacgga ttctttatag tgtcttattc tttagaagtt ataataaaaa
    10381 ctgatggctt tccaatatta gctactgttg gtgtaataat atctgctctt acaaacatca
    10441 tacttgatta cttgtttgta attgaatttg gatggggtgt aaaaggtgct ggtattgcaa
    10501 ctggtttgtc tcaagtcttt tctactattt tcttcttaat acatttttta agaaaaaact
    10561 ctactttgaa tttttcaaaa tttagaattg attttaagac acttagaaaa attgtattta
    10621 taggttttcc tgattcaact actgaattaa gttgtggtat agttgtttta ctttttaact
    10681 taagtcttac taaatatatt ggagaaaatg ctcttattta ttatagtgtc ataaattata
    10741 taaatacatt agtacttatg actatgatgg gtataactca aggtatgcaa ccacttacta
    10801 gcttttatta tggtgcagga aatatagata atgtaaaaaa attattaaaa atggggataa
    10861 aagccacaat aatagcatca gttgctgtat ttgcaatttg tatggctttt tcaggaccaa
    10921 tagtctcttt atttatacat ccagaagaaa caatgctatt caatgaagga gttcgtgtat
    10981 ttaaaatatt ctctatatct ttcttattgg taggaattaa tgtaataatt tctggattct
    11041 ttgtatcagt agaaaaacca tcaatttcta ctgttatatc tcttggtaga gggcttgtaa
    11101 tagttgtttt aagtctaatt tcaatgatac taatatttgg tggacaagga atatggatga
    11161 ctactattgt ttctgagttt atatgtctta ttttatcatt agtttttcta aaaaagaatt
    11221 tttctacatt agactctaat ctaaataagg ttgcttagat gataagagtg acaatatatg
    11281 aaaatatcta aataaaaaat acctattata caatcttgca aatattaaac ctttgcaaaa
    11341 attaggatta tataataggt attttattta tgtaattatt ttatttctaa atatccatag
    11401 ccatctttta ttaaagttcc ttttacttca tattctacag gttttttaaa tattggacca
    11461 tctacgacaa atgtatgata ctctccatgt tctccacatg ggtctgcacc agcatttttt
    11521 atcttctcca caagttctct agttaatttt tttcctaaaa attcctctcc taagtgcttt
    11581 aagtttactt ttgttacaac actacaaaat ccactgtcta taaattcata tactaactcc
    11641 tctctactct cttgccataa tggtaaatct actttcatac caactgcttc acatctatca
    11701 gtatcccatt ttctatgact ttctatatct atatctccat atgcacatat agttgctccc
    11761 atgtttttag cttttattaa agccctctca aagctctctt catatccttc tacaccacac
    11821 tcagcaacta ataaaggtaa tcctatattg ctacttactt gcttaagcat attatattct
    11881 aaattatgtg tccatgactt tccatgttct tcacttatag ttgtcaagag tgccacaggc
    11941 ttatatcctt ttttcaacat tcgatttaat gctaatatac tatccttacc accactaaat
    12001 gacattacaa atttttcatc agccaatttt aacacctctt tcatctaact ttgttttatt
    12061 aacataatta taccaaattt ttgagttgca tagcaaatta tattaaattg ttaattcact
    12121 ataaagaatg tccctaatat taaaagtata acccctatgt atttgtataa atgcattgtt
    12181 tcttgtccca aaattacgct atcaataaaa aatccaacta aaacttggga tacaagagtt
    12241 aatataagtg tatttgatac tcctaaattt ggaattgcct ttactgtaaa atatattatt
    12301 agacctccaa atactcctcc taataacaac ttataattaa aaccatttat agatactaga
    12361 cactttaagt cacctgtaac tattatactt gccaaaaaaa atattgctcc tacagttaag
    12421 cttataaagg tggccactaa tgctgttgta ctttttccta gctctccatt tataaaagct
    12481 tcaagagctg tagaaccacc tgctaataat gcaaatatta ttgcccataa ttttgtcatg
    12541 gacaaacctc cttctatata attctattaa tagaagttta tgatacaacc tattaattaa
    12601 ttactaagta tagtttaatt tttatacact aggctttatt tggatatgaa tttctgttat
    12661 aattgttcta tcgattttgg aggtgatttt atgtacacaa attttaatat cgattttaat
    12721 aactttaata aaaaagaaaa tgccattaaa tttatgttaa tgggagtatt actcattata
    12781 ttaggattat tatgccttac atttaagaca ttaggtataa aattaatttc ttggactttt
    12841 ggtattgcac ttttattctt tgcatattta aatttaaaaa atattaatga actaaaaaga
    12901 tatgctacta aagaagaaat taaaccctct attaatatcc aatgggttct aataattgca
    12961 tgtatattac tttttgtatt tcctcaaaaa atacaaagca tattttcatt atttttagga
    13021 ttttatttaa tctttaatca actagtagca cttgtaaata gtaagaataa tccttattct
    13081 aaatttacaa cttggaatat agttaagatt ctatttggta tatgccttat attatctcca
    13141 ctttttttat ctagatttat agtatcgata atgtcttttt tcataatatt atttggacta
    13201 gttctatttt tctctggaaa tacagcgaga aaatattaat tattttctcg ctgtattttt
    13261 atattactta aaatagaatt tgtttaagtt atctatttgt ggtaatatgt tttttgtaca
    13321 agctcagcgt acaattttat tagaaatagg tgattttttg gataaattta aatatgcttt
    13381 tgataataaa agatatcata cttggaatta ctatcttaga aatacttttg gagaaaaagt
    13441 ttttaaggta tctataaatg caggatttac ttgcccaaac attgatggaa gtcttggtta
    13501 tggcggatgt acctattgta gcaaagaagg ctcaggtgat tttgctggaa accctaaaga
    13561 taatcttatc agtcaatttt ataatataaa agaaatgatg ttaaaaaaat ggcctcatgc
    13621 aaagtatatt ggctactttc aagcctatac aaatacgtat gctcctcttg aagttttaaa
    13681 agaaaaatat gagactatat tagaacttga agatgtcata ggtctttcaa tatccacaag
    13741 acctgactgt ttgccagatg atgttgtaga atacttgtct gaactaaata aaagaaccaa
    13801 tttatgggtt gaacttggac ttcaaacaat tcatgattct acatctaaaa ttataaatag
    13861 aggtcatgat tataaaacat ttgttgaagg tgttgaaaaa ttaaagtcta aaaatataaa
    13921 ggttgtcgta cacattataa atggtcttcc tggtgaagat tataatatga tgatggaaac
    13981 agcaaaggca gttggtaaaa tgggtgtaga cggtataaaa atccatctat tgcatgttat
    14041 aaaagatact cctatggaaa agatgttaca aaatggtatg ttaactctta tggaacaaga
    14101 tgaatatatc aatcttgtct gtgaccaatt agaaatactt cctgaaacta tgataatcca
    14161 tagactcact ggtgatggta agagggatga actagtaggt cctatatgga gcctaaaaaa
    14221 atgggaaatt ttaaataaaa ttgatgatac tttaaaggct agaaattctt atcagggctg
    14281 taagtttgtt taatacaaaa taataactat agaaatggtt atacaaatat agtcctttct
    14341 atagttattt agtatttaca aaacaaaata ttaatttgta catttataca atatataata
    14401 aaaaatagat agtctaaact gtatctactt atattttatt tgtactgtcc actttattct
    14461 ataaaattta taaatagtta ttttttaata ccaaatataa tttggtataa tttttgttgg
    14521 aatctacaat gtataccaat aaatcctttt taagtatata attctaaata ctttcagttt
    14581 tttcaatctg ctttaacttg ttggacttat aaaatattct tatttcatca taagaaagat
    14641 taagtgctac tactgcagat attaacatca acatcactcc aaataacttc cctaaggaaa
    14701 aactttctcc taatatagtg caaccaatta ttactgaacc aactaattct actgatgcca
    14761 caacacctgc aactgacaac tctacacctt tagatatacc tgctgcataa aaaatatatg
    14821 caagtgcagc tggaacaatg cctaacatta gcatacaaga taaaacatct aaatccttag
    14881 cataatttaa gatttcccaa ggtcttgaag aaggaatcat aaatatagct ccaaataaga
    14941 aactgtatac taatattgtt gaacttgaaa attcctttaa agcattttta ctaatagttg
    15001 gcataagtgc atatgctatt gctgataata ctccaagtaa aagcccaaaa gcgtttaaac
    15061 cttgaaaatc caaacgtcca ccagtcactg ccataattgc accaataaaa caaagaataa
    15121 gtgaaaataa tttgcttcta gtaatatttt ctttataaca taattttgaa aatattgcta
    15181 aaaacagtgg tgatgtatat agtaaaactg ctgcaattga tacacccgca atgtcaattg
    15241 ctttaaaata acataaatta aacattgcct gacatataat tcctattatt actgaatata
    15301 taattccttt tttgtttatc tttaagattt gtggtgtcct tatagatgaa tatacgccta
    15361 aaactataaa tccaaaaaac aatcttgtaa atgctacttg ttctgatgtc agtccatttc
    15421 ccattaaaaa ctttccaaat aatccaagtg tagcccataa taaccctgct attgctataa
    15481 atacataccc tttacttttt tctcccattt taaattccct tctattttta tttagtcaat
    15541 ttacactctt ctttttaagc aaaaactatg ccatcttaaa tcaatatata tcgactctaa
    15601 aaccctagtc tttccaataa tactaataaa ttttcaattt ttttatattt atttttcgct
    15661 tactttaagt tgatttagac ttattttaat tgtaaaatga gttatatata acttattt
//
LOCUS       DAMJZE010000092        15493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJZE010000092 DAMJZE010000000
VERSION     DAMJZE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.093"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            192..1268
                     /locus_tag="PC541_002373"
     CDS             192..1268
                     /locus_tag="PC541_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2839832.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1371..2213)
                     /locus_tag="PC541_002374"
     CDS             complement(1371..2213)
                     /locus_tag="PC541_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2839833.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2397..2969
                     /locus_tag="PC541_002375"
     CDS             2397..2969
                     /locus_tag="PC541_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2839834.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3132..3404)
                     /locus_tag="PC541_002376"
     CDS             complement(3132..3404)
                     /locus_tag="PC541_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839835.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3531..4403)
                     /locus_tag="PC541_002377"
     CDS             complement(3531..4403)
                     /locus_tag="PC541_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2839836.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4699..7806)
                     /locus_tag="PC541_002378"
     CDS             complement(4699..7806)
                     /locus_tag="PC541_002378"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2839837.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7899..8131)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8306..8833)
                     /locus_tag="PC541_002379"
     CDS             complement(8306..8833)
                     /locus_tag="PC541_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2839838.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8868..9653)
                     /locus_tag="PC541_002380"
     CDS             complement(8868..9653)
                     /locus_tag="PC541_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2839839.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9663..9920)
                     /locus_tag="PC541_002381"
     CDS             complement(9663..9920)
                     /locus_tag="PC541_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2839840.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9939..10397)
                     /locus_tag="PC541_002382"
     CDS             complement(9939..10397)
                     /locus_tag="PC541_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2839841.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10437..11141)
                     /locus_tag="PC541_002383"
     CDS             complement(10437..11141)
                     /locus_tag="PC541_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2839842.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11151..11786)
                     /locus_tag="PC541_002384"
     CDS             complement(11151..11786)
                     /locus_tag="PC541_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839843.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12167..13207
                     /locus_tag="PC541_002385"
     CDS             12167..13207
                     /locus_tag="PC541_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2839844.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13670..15025)
                     /locus_tag="PC541_002386"
     CDS             complement(13670..15025)
                     /locus_tag="PC541_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2839845.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15051..15320)
                     /locus_tag="PC541_002387"
     CDS             complement(15051..15320)
                     /locus_tag="PC541_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2839846.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         4921 a         2691 c         1794 g         6087 t
ORIGIN      
        1 ttattacttt tatagaattt tcacaataaa aagtcatttt acttccgtat ttaatgcaaa
       61 agtagaaatt aaacttaata taaaaaatat ataacaaatt ttttttatat ttctagaaga
      121 aaagtatact cttttttgtt taataatgct aaaattgatt tgtattgaat tttgattagg
      181 aggaaattat tatgaaagct caaagattaa atgctgtact tgaacaaatg aaaaaagatg
      241 atatttctca aatgttagta agtgacccta cttctatatt ttatcttaca ggagtactaa
      301 tccatccagg cgaaagacta ttagcattat acttaaattt aaatggcaat aataaattat
      361 ttataaatga attattccca gtttcagaag atttaggtgt tgaaatggtt tggtttaatg
      421 atactcaaaa tccagtagaa ataataactg aacatataga caaaaatgct actatgggtg
      481 ttgataaaaa ctggccagct agattcttat taaacttaat agaattaggt ggaggaagta
      541 agtttgtaaa ttcatcttat ataatagata ctttaagaat gtgtaaagat gaagaagaaa
      601 aagagttaat gagaatagct tcaaaattaa atgacaaggc tatggaacaa cttaaagcta
      661 ctgtttctgg tgaacttaca gaaaaacaac ttgttggtaa attatctaaa atatatgaag
      721 acctaggtac tgatggattt tcatttgacc ctattatagg ttttggacct aatggagcta
      781 atccacatgg tgaacctgga aatgcacttg tgaaaccagg agatgcaata atcttagata
      841 taggttgtat aaaagataac tattgtgctg atatgacaag aactgtattc tataaagaaa
      901 tacctgaaaa aggtagagaa atatttgaaa tagttcttga agctaataag agagctgaag
      961 ctattgtaaa accaggtgtt agattctgtg atatagatgc tgctgctcgt gactacataa
     1021 ctgaaaaggg atatggacag tacttcactc atagaactgg tcactctata ggacttgaag
     1081 ttcatgataa aggagatgta tcttctataa atacagatac tgtacaacca ggaatgatat
     1141 tctctataga gccaggtata tatttaccag gtgagttcgg agttagaata gaagatttag
     1201 tacttgttac tgaagatggt tgtgagatat taaacaaaca tgacaaagaa atatgtgtag
     1261 ttggttaatt ctaaaatcaa tttgataaaa ttaaaaagcc atataaaaat gttttaaaca
     1321 tttttatatg gctttaattt ctcaatctat gatttatttc aatccattat ttatattgca
     1381 gttattaaga ttgcaccctt tgaagtatat gtccccatca ctgaaccaat ttcacagatt
     1441 acaacatctt tgtattttac atccttttcc ataacttccc ttaccttttg agccttttct
     1501 gggcaattag catgtcctat aaataaaacc ttttcttctt tatctttaat ttttgactct
     1561 actaaagtaa tcagcttttt caaactgttt ccttcacctc ttgccttatc tataggagtt
     1621 acaacgccat ctgcaacttg tattatcgcc ttaaaattta aggtatttat aatttttcct
     1681 gctattggat ttattctacc acccttaata gcattttcta aagtttcaag tgttccaaaa
     1741 aatggaggat tatctttata tttatttatt attgaaacaa tctcttcaat ttcttttcct
     1801 tcttttatta atattgctgc ttttaacact aattgtccta ttccaattga cccactaagt
     1861 gaatctataa cctcaacttt cttgttattt ccttcctttt caaacatatc ttttgctaaa
     1921 agtgcactat tataagtccc agataatcct gaacttatat taatcactaa tacactttct
     1981 tcctgacaat ggtatgcttc taaaaattta tcaggagaag gacatgatgt ttttggtagt
     2041 actttactct cactcatttt tctatagaaa gtttcattat ctatttcctt tccagatata
     2101 taactttctt ctccaaaaga tacatttaaa ccaacaactt ctatgttgta tttttctatt
     2161 atatcatcag gtaagtcaca tgcactgtcc actatcaatt ttattttatc catattaacc
     2221 tcactataag atattatttt atttattcag tcggtcgtac gaccgattta attattattt
     2281 tatatattga atgaaaaaaa gtcaatagat atagattctc gctttattga caataaatat
     2341 ccataagctt ataatgttat taatttgtta taaatataca tggaaaggta ggttttatga
     2401 aaacaaataa tatgaaacag gctattttat catctgcaac caatttaata gccaaaaatg
     2461 gagttcaaaa tactagcctt gctgatattg caaaagatgt aaatataagt aaaggtactt
     2521 tgtattatca ctacgcatct aaagatgata ttatatatga tattgcggat actcatttag
     2581 aagttataac tagtgctata ctaaactgtg taaaaaatgt caaatcaaaa aattctcaaa
     2641 tagaaatggt taacttaatc ttagagaaaa tatctactat agaaagtcgt ggtagggtac
     2701 atatgtatct tatttgtgag gcaattactt caaataatga tttaaaggaa agaataagat
     2761 taaaatatat tgaatggaga actactctta aatctgaaat tgttgaatca ctagaaaaat
     2821 ataatagtag cgaaaatgaa caggatgctg aatcattttc ttttttactc atgtcaatag
     2881 ttgatggttt agttgtccaa agtttattaa aaacagaaaa aataccttat gaaaatatag
     2941 cttctttttt agttaatcat tggatttaaa cttaatactt agataattaa taaaagtgaa
     3001 ctatttaagt tatctacttt aacagttcac tttcttattt attctcaata atttttactt
     3061 aaaacattca atttttattt aaaccttcat ggaatcacaa aataaaaatc tcttataaat
     3121 ttaaaatgtt tttatttatg ttcatgtgta tgcccatctg aatgttcatg accatgtgta
     3181 tgtgtatgct catggctatg ctcatgagta cctgaaaaac catcatcatg attatggtcg
     3241 tgacctacac catttacatg ctcatgttca tgcccatgac tgtgagaatg ttgatgtgaa
     3301 tgttcatggt tatctcctcc acagcatcct tctcctccag tcccacaaca cccacttgta
     3361 tgtggattat gatgatttct tactacaaat ctctctctta acataattct tatacctcct
     3421 agtatattat tgagtacttc atgtacccca ttaactacag tatacatcta actaggtata
     3481 atctcaatac atatggcatt ttttaacata tataattaac atatataatc ttaaagaaat
     3541 ttatccttaa aacctccacc ttttacttct tcaacgttat tgacagaaat aaaagcttca
     3601 ctatccatat cataaattaa atctttaaat ttacctattt catttgcaga tattatgcaa
     3661 taaatcatct ttttcttatt ttgtgtatat gcaccttctg cctccaaaaa agtgactcct
     3721 ctacccattt tattcattat cactttggat atttcatcat atttatttga tattaaaagt
     3781 atagattttt ttctatcaaa gcaatctttt cctatatcca tagtaaaaga aattatatac
     3841 attgtaatta aagtgtacat caccaattta gctccaaata aaaaagctcc tgcacatact
     3901 acaaaaaaat taataaataa aaatgtattt ttcataggta tatcatactt tcttttcatt
     3961 atggctgcta ttatatcaag tcctccagca gtagctcttg ctttaaaagt taaccccata
     4021 cctataccag ttaatgcacc accataaata cactgtagca tagtatcatc tattgggaaa
     4081 tatttagcaa tatcttgtgt tagccccagt gctagagaaa atagtaacat attgacaaaa
     4141 cttattagca aaaatttatt atcaaaatac tttctagcta taataaatat tggtatgttc
     4201 atcaagaaac ttgatattcc ctgattaata ccaaataatt tgtataatat aacacaaatt
     4261 ccagctactc ctccacttaa taacttggct ggtgctagat ataaatttat tcctaaggtt
     4321 gatagaatca tacccaatat aataagacaa tattgtaaaa atatattatt atcaatactt
     4381 agctttttac tttcaccata catcaaaacg cacccctttg cataataaat aaaccaccct
     4441 gcataacaca cgataaattt acaaataaat ttatcagaag tagtttccta aaaatattat
     4501 tcatcctcac ttaaacgttt attcattaat attctatcac atgacataaa taatttatat
     4561 gatttatata gatttattta ttgtaaattt tggaatatta aaacatttta ttgaatagat
     4621 aattatatta cataaaaaat ccctatagaa atctataaac taatttctat agggattttg
     4681 gttaactaac tattattttt atttttttat aacttttata cctgtcatat ggtcatttaa
     4741 gttttgttcc tctaaagatt tatcatctac tctgtttatt tctaaagcta aagtttcaga
     4801 cttgatatat tcattaaaat ggtcaacagc cttagcaatt tcatcatcac cacagtattc
     4861 tattactata ttatctaata cttcaaagtt attagatttt cttaattgct gtactttaga
     4921 tataaattct ctagcataac cttcattaat taactcttca gttagcttag tatctagtat
     4981 aacaaaaaga ttgttttcaa cagatacatt aaatccttcc ttagcagtta tacctattaa
     5041 tactaaatcc ttattaaatt caaaagcttc accatctaaa tcaactgtta aagtctcacc
     5101 tttttcaagt ttattagcag tttcatgagc atttaattta cttaatgctc caacgaataa
     5161 attcatctta gaacctaata atggccctgc ttccttaaag tttggtttta aactaaagtt
     5221 catatactca tctaaatcct tagcaaatat tacctcttta acattaagct cttcttttat
     5281 taaatcaact acatcactta ttgtatcttc aaacttacca tctactaaca ctttttgtaa
     5341 tggttgacga acttttattc tttctacttc tcttgaagct ctacctaaag taactaagtt
     5401 tttagctaaa tccatttttt cttctaattt agtatctact aactcttcat tacatttagg
     5461 atattcagct aaatgaacag atacttctcc tgttaagttt ctatacattt cttctgacat
     5521 atatggtgca aatggagcta tagctctaca aagaccatgt aacaattcat aagttgtatt
     5581 gtacactgct tttttatctt ctgttaattc agttgcccag aatcttcttc ttgaacgtct
     5641 tatataccag ttagataaat catcatttat aaaatcttgt atcattctta aagttttatt
     5701 taactcaaat atagctaaat tttcttctac atctttcatt aaattattga acttagataa
     5761 tatccatcta tcaagttctg gtctatcttt atattctaca aaaaattcag ttggatttat
     5821 atcatctgtg tttgcatata aagtaaagaa gttatataca ttcttcatag tacctataaa
     5881 tttactttgg atttctttta atccatccat atcaaattta gttggagtcc atggtggaga
     5941 aacatatagt aaataccatc ttaatgcatc tgctccatat tggtcaaata attccattgg
     6001 atttactgta tttcctcttg atttactcat tttcttacca tctttatcaa gtacaagatc
     6061 tggaactaaa acactcttat atggtgcttt tcctaataca aatgttgata ctgcaagtaa
     6121 tgagtagaac caacctctag tctggtctat accttctgat atatagtcag ctgggaacaa
     6181 ttggtcaaag ttttccttat tttcaaatgg atagtggtgt tgagcaaatg gcatagcccc
     6241 actatcaaac caacagtcta taacctctgt tactcttgtc attggttttc cacatttttc
     6301 acattttaag tgtatatcat ctacatatgg tctatgaagt tcaactgttt ctggatttac
     6361 atcttctata gctttttcag ctaattcagc tcttgaacca actgaatctg tatgaccaca
     6421 ttcacatctc catataggaa gtggagttcc ccaatatcta gttcttgata ttgcccagtc
     6481 atttagattt tctaaccagt ttccaaatct cttctctcct acaaaacttg ggaaccattt
     6541 tacaccatta ttattttcta ttaatttatc tttaagttta gtcatctcta tataccagct
     6601 tggttttgca tagtatacta atggagtttg acatctccag cagtgtggat aattatgaac
     6661 tactttttct ttgctatata atttattttc agaagctagc cattttatta tttcaacatc
     6721 tagcccatct tccattacaa atctaccttc ccaaggagta gatgtaaatt taccagtttc
     6781 atctactggt tgaataactg gtaaatcata tcttttacct gtgttgtagt catcttcacc
     6841 aaatgctggt gctgtatgaa ctataccagt tccatcttca gtagttacat aatctcctac
     6901 tgtaataaaa aatgcttttt tgtcaacttc tacaaaaggc attaattgtt catattctaa
     6961 accttctagg tctttacctt tcatttcttc taaaacttca taatcttcac ctagtacttt
     7021 atcagctaaa ggtttagata cataatatac ctcatcacct tttttaactt ttaaataatc
     7081 tacatcagca tttactgtta atgaaacgtt tgatggtaaa gtccatggag ttgttgtcca
     7141 tgctaagaag tattcatcag catctttttt cttaaattta gctataactg tattagtttt
     7201 tacttcttta tatccttgtg caacctcatg actagcaaga ccagttccac atcttgggca
     7261 ataaggaagt attttatgtc cttcatatat atatccttct ttgttgaatt tgtttagtat
     7321 ccaccataca gactctatat agttattatc taaagttata tatggattat ctaaatcaac
     7381 ttcataagcc attctctcag tcattattct ccattgtttt tcaaatgaga aaactgactc
     7441 tctacatttt tggttaaatt cagctattcc atattcttct atttgttgct tgctagtaag
     7501 acctaactct ttctctactt gtatttcaac tggtaatcca tgtgtatccc aaccagcttt
     7561 tctctttact tgataaccag acattgtttt atatctacaa actgaatctt taagcgttct
     7621 agataaaaca tggtgtactc caggatttcc attagctgtt ggaggtcctt catagaatac
     7681 gaaacttggg tcatctttcc ctttttctaa agttttttca agtatattta tgtctttcca
     7741 ataatcaaaa acttgtgctt ctgcttgttt tacagaactg tctaccaatg gcttaaattt
     7801 cgccatgtaa atcacccttt cctactaaat attataattc caaaattttt tattctttca
     7861 cataaattta aatactaaaa aactcgcccc ctttaaacca aagggacgag ttataaatcg
     7921 cgttaccacc ctaattctat atgtaaacta aatactagct acatataaca ctctaatagt
     7981 ataacggtac taaccggcac agcttagtag aatttaatcg ttcagcctgc agctcaagag
     8041 tgatttttat ctttataaat tcattggttc tcagcatacc caattctctg taaaattatc
     8101 taaagataaa tgtctctatc aacgctttta aaaatttatc tgtgtttata tgaaattcta
     8161 taacaaataa tttgaatagt caatatattt ttgaaaaata taataatttc tatttttctt
     8221 acatttttat cctattttta atctttgtta tagatttctc aaactcacac aattattcta
     8281 cataaaaaat aaacaaatta caagcttatt ctaaagttgt agcagcttca tcattactat
     8341 aattatacac tgttgttcct tcaaatgcat ttacacattt ttcatctaca tcataaaaaa
     8401 tctcatcaac acttcttatt tcatcttcta acagtgattt aaatttattt ctaaatatct
     8461 taaattcttt gactaaagca tcatactctt ttcttatctc tataacacgg ttattagctt
     8521 gttctataat ttgtctagct tttaaatcag cttcttcaac aattatctta gcctttttat
     8581 tagccgcgct acaagtgtct tctgctgcac tttgagcagt tattaaagta gcttttagtg
     8641 tttcttcaat attttcatac ttgtttactt ggtcttgata taaccctaat ttttctttaa
     8701 gagctgtatt ttccctacat agagattcat aatcttcctt gatgatatct aaaaattcat
     8761 caacctcttc atctctatac cctcttaaac cttttttaaa ctctttattt tctatctcaa
     8821 ttggagttag cattacattt cctcccagct ataatttaaa ttagtcctta tacaattaat
     8881 cttgcttgaa cttttatttt tcctttttta gtaatatctc ctatttgtgt taccttagct
     8941 cttcctttgc ctctaataga aatcaaatca tcgtttttta ctattttaga agttgaagtt
     9001 atcttttcat aattgacatg gactttttct ccatttatgt attttgcact atcttgtcta
     9061 gatatattgt aaataccact tatcacacaa tctaacctat ctgaagatac tgtaaatgat
     9121 acttctttat attttgaaga actattaaca atattttctc tggaaatttc ctttacaatt
     9181 acattatttc ttgaaacctt ttctaagttt acaattataa aatcgcaaat atcactactt
     9241 acaactactt gacaaaagct atcatgtata attatatctc caattttttc tctttttata
     9301 cctagaccca gtattgaccc taagtaatct ttatgagata tatttttaaa tttgaaattc
     9361 ccttctattt gtaaaaatct aagagtatct tctatatctt catattccat gtaaaatgga
     9421 taaataaata cagtgcttct ttcagcttgt tcataacctc catccacact gtattttatt
     9481 tcatttgtag aatttaatat tgctacagca tttttcactt catatggatt aagaaactcc
     9541 gtagacttaa catcatagtt ttttatacaa ctattagcct tgtctatcac tttaaacatt
     9601 ttatttttca agtctatatc ctttatgtga tttgttagtt ttaatttatc catcctatca
     9661 ccttagaata taaccaatac taattgtctt aggaacatca ataatactat tgcaattaaa
     9721 ggagaaaaat ctataggtcc atttgtatat ttatacatta tagaccttat tggcccttct
     9781 attggctctg taagattttc caaaatatca aaaattttac tttgtcttcc atttggaaac
     9841 caagtcatca agctttttat aactatcatc catgctatta tatctaaaag ataataaagt
     9901 gcaattctta ttgttcccat atattaaccg cctttttact atttattttg ccaagggaag
     9961 aatgctttac tttctaattc ttttttcata tttgcatcta tatccacatt attaggagct
    10021 agtataaata ttgatttaga aaccttttgt atacttccat ctaagacata aactgcccca
    10081 ttcataaagt taaatataga tttacgaact tcagaattag tcaatccttc caaattcact
    10141 ataactgccc ttctttgttt tagatggtct gctatagttg cagcttcatc ataaacctta
    10201 ggttcaacta ttacaacttt catttggcta gttgtatgta aatttacaat tttatttgct
    10261 tttgcagtac taaagccact gttcatttct tcttcttgaa ctatatcgtc catgccactt
    10321 tcatattcat cttcaatgta atcttcttct tcatcaacta tccaattctt aaatttagat
    10381 attattccat ttgacattat taacttcctc cttaaaattt attcaaattt gagtaattat
    10441 tttttattgt agtttctttc tccaaagatt gatgttccaa ctctaacaat ggtagcccct
    10501 tcctctatag caatttcata atcgtgactc attcccattg acaaaacatc catccctaca
    10561 ttagggatgt ttagttcttt tatttgtttt gataaatctt taagaccttt aaatacacct
    10621 cttatctcat cttcatcttc tataaaaggt gccattgtca ttagaccctt tatttttata
    10681 tttttatatt tttctgacac ttctttgaca aagtctacaa cttcttctct atcaagtcca
    10741 tgcttagact cttctttaga tatattcact tgtactaagc aatctatttc tctgtttatt
    10801 tgttctgcac gtttttgaat ttcttcgcat aatgcaattc tatctaaaga gtgtatcata
    10861 caaactttat cgattatgta cttaacttta ttggtttgca aagttcctat caggtgccag
    10921 tttaatttat cacctataat atcatacttt ctagctaatt cttggggttt attttcacca
    10981 acagaagtga ttccttccct cattgcctca ttaactaagt ctacatcaac agtctttgtg
    11041 actgccaaaa gggtaatctc tttgctgtct ctatctactt tttttgcagc tttatcaatt
    11101 ttgtttctta tctcagacaa attatcttta atagaaccca ttattatcac ctactttatc
    11161 cttatatttg tgtttatact gttaggtctt aaaattattt tgtcatatag attaacactt
    11221 gtaactccat ttatgtcatt tttataataa tccacaaata cagaagattc attctcataa
    11281 gttattcctt ctaatatgac aaattttaca ctttgtgatt gttcatcaat tacatataca
    11341 ccacgtttat tgtcttttgt ttttatagaa gttttaggta tttttaaaca ttccatctga
    11401 ttatatatta tatcaaattc ttcgacactt gtatcctgat ttgcaacaat ttggttactt
    11461 atttttaatt ttaccattat tttattatta ttttcatata ttttgtaaac ttttgcatct
    11521 agtttttgct catgatattc aattattaca ttttgatttt tttcaaagtt attagaatct
    11581 tctttactga tgtatgtaga tagataatta ctttggtcat tggcatcttt ttctatagtt
    11641 ccttttacat gaatctttgc atcaactttt tcatttttta acaccaacgt atctacattt
    11701 ttgctaatta tcattactaa tatattaaat actaaatata taagaatacc aagtatcact
    11761 aatctggtat attttacctt tttcaaagac tctcccccta taaaattact ttcattataa
    11821 gtacttcttc taaaaaatgt ttattccttt tttataagat aatattttac gttctaataa
    11881 ttattttttc catattaaat ttatttttta tcaatcttcc tatacaagac tgcattattt
    11941 atatataatt gtattataaa gtataagttt aatttacatt ttatacgtta agcttacttt
    12001 atatattttt atagttattt ttatttttag atgcaatata aaaccaatta agatttaaaa
    12061 taacttttgt tatgttgaca tttatttagg taatcattta tgaattagga taacgttatc
    12121 acaacaaaag tttatgtcct aaaaaaaata caaggaggat tttcttatgt cagaaatttt
    12181 attaggaaca ggtttacttt tattggttct atgttgtttc acaattttta gttacaaggc
    12241 cccatatggt atgaaggcta tgggagctct agcaaacgct gcatgtgcta ctttcttagt
    12301 agaggcattc aacctttctt tacttggtga tgttttaggt atggaatttt ttagaagtgt
    12361 tggagctgct aatggtagtc ttggtggagt tgctgctgct atacttgtgc ctttagcctt
    12421 aggtgtatct ccagtttatg cagttttaat aggattatct tgtttagatt tttctatatt
    12481 atctggattc gttgctggat actttgtttc tttcttaatt aagtttatag agaaaaaagt
    12541 gccacctgga gtggatttag tggttgtaat tcttctatgt gcaccactat ctcgtcttat
    12601 agcatcaaat gtagacccat tagttaatag tacactacta caaattgggc aagttttgat
    12661 agaagctacc aacattagcc ctataataat gggaattata ctaggaggtc taataacagt
    12721 agttggaacg tctccactta gttcaatggc tcttacatct ataattggtc ttactggtct
    12781 tcctatggca ataggagcat taggtgcttt tggttcagct tttatgaact caacactatt
    12841 tgcaaaattg aaattaggtt ctaaaaaaga tattatatct gttgctattg aaccattaac
    12901 ccaatcagac ataatttcag ctaatccaat acctatatat tctactaatt tctttggagg
    12961 agccttttct ggtatcattg tagccttgat gggtcttgta aacatgacac caggtacagc
    13021 tacacctgtg gctggttttg ctataatgtt tgcttataat ccagttcaac aagttcttat
    13081 aacagctggt ttatgtgctt tattcaatgt aattgcagga tttttaggtt caaaaatatt
    13141 cagtaccttt aaagttctta ctgctggtga aatacgtggt actgatgtag aagaagaaat
    13201 tgcttaaaaa acaataagat ttataaattt atatctcaag tagtagagtt ttatattgta
    13261 ggcaatttta ttaattgcaa acgttttatt ttttatttat gtttatgatt attatgtgtg
    13321 ttgaatattt agataaattt ttatttatta tttttattta gatttttgtt tagtttttta
    13381 tttggttgat atattaggaa taaaaattag tatagtttat tactagttag atggcataac
    13441 tttttagata cataaaaaat tttatattaa ttagatatta ttaaaataaa agcgatacat
    13501 gaatttttaa atgtccatgt atcgctttat ttttgccata atcttaataa ctgattttta
    13561 aagtttatta tcttaacaaa gttcattatc ttatataata tttaaatgca taggcttctt
    13621 taatttaacc atctaaatat ttaattaaat attattatat gtaatataac taatttttaa
    13681 atgaatgtat aggagctgga atccttcctc ctctttgagt aaataattca gatgaatttg
    13741 tatttatagg cattactggt gctcgtccta aaagtcctcc aaattctacc atatcaccta
    13801 catcacatcc tggggctggt ataattctaa cggctgtagt cttattatta atcataccta
    13861 ttgctgcctc atcagctatc atggctccaa gcgtacctgc tgtagtattt cctggcacag
    13921 ctatcatatc aagacctaca gaacaaatag cagtcattgc ttcaagctta tctatactta
    13981 aagcaccttt aataacagca tcaatcatac ctgcatcttc agatactggt ataaacgctc
    14041 cactaagtcc tccaacatgt gaacatgcca ttactccacc ttttttaaca gcatcattta
    14101 aaagtgctaa tgctgctgta gtcccatgag taccaacaac ttcaagaccc atttcctcta
    14161 atatatttgc tactgaatca ccaatagcag gtgtaggagc tagtgataag tctattatcc
    14221 caaatggaac atccaatcta ctagaagctt cctttgccac aagttgtcca actcttgtaa
    14281 ttttaaatgc agtcttttta actgtttctg aaacaacatc aaaaggctct ccctttactt
    14341 tctctaaagc tctcttaaca acaccagggc cacttacacc aacatttata attctatcag
    14401 cctcaccaac accatggaaa gctcctgcca taaatggatt atcttctact gcattacaaa
    14461 atactactaa ttttgcacaa ccaaaacctt ttgcatcttt tgttaattct gcagtttctt
    14521 ttatgatttc tcccatttct ttcactgcat ccatatttat accacatctt gtagaaccta
    14581 catttactga agcacatact ttattagtac ttgctagagc ttttggaatt gacttaatta
    14641 gtatcttatc accttttgta tatccctttt gaactaatgc tgagaatcca ccaataaaat
    14701 ctatacctaa atcctctgca gccttatcca atgtctgtgc aaactttaca tagtcatttt
    14761 catctgttgc acctgcaata atggaaattg gagttactga taccctttta tttacaattg
    14821 ggataccaaa ttcacgctct atatcttttc caaccttcac caaatcttta gcagaactca
    14881 taatcttatt gtatattttt tctctagctt tatctccatc agggtctata caatctaata
    14941 aagaaatccc catagttata gttcttatat ccaactgttc ttcatctatc atctttattg
    15001 tttcaagtat atttcctaca ttcactatta tcacctcccc ttactgtcta ctacagatta
    15061 tgcattgaat taaatatttc ttctcgctga atttttacat ccactgataa tttcttacct
    15121 ctttcaacca agtcctcaac cgttttctca aaagaattta atgatttctc taaatcaact
    15181 atcataatca tagtaaaata atcctgtaat attttttgtg atatatctac tatgttgata
    15241 ttttgcttgt ataattcatc actaattcct gccactatac ctggtttatc ctttccaatt
    15301 actgttaaaa ttgctttcat aataatatcc tcccttattt ttttataaat cttatatttt
    15361 tacaaattga actatgtttt aacatatatt tcatttattt gttagtaaac cactttttat
    15421 gtatatattt aatatatttt aatacctata cattcatttg tcaatttttt ttcaatattc
    15481 tattattttt ata
//
LOCUS       DAMJZE010000093        15460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJZE010000093 DAMJZE010000000
VERSION     DAMJZE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.094"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            25..876
                     /locus_tag="PC541_002388"
     CDS             25..876
                     /locus_tag="PC541_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2839847.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1009..2622)
                     /gene="ggt"
                     /locus_tag="PC541_002389"
     CDS             complement(1009..2622)
                     /gene="ggt"
                     /locus_tag="PC541_002389"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2839848.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2847..4784)
                     /locus_tag="PC541_002390"
     CDS             complement(2847..4784)
                     /locus_tag="PC541_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839849.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4771..5538)
                     /locus_tag="PC541_002391"
     CDS             complement(4771..5538)
                     /locus_tag="PC541_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839850.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5826..6500
                     /locus_tag="PC541_002392"
     CDS             5826..6500
                     /locus_tag="PC541_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839851.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6524..7528
                     /locus_tag="PC541_002393"
     CDS             6524..7528
                     /locus_tag="PC541_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2839852.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7640..8869
                     /locus_tag="PC541_002394"
     CDS             7640..8869
                     /locus_tag="PC541_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2839853.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8877..9551
                     /locus_tag="PC541_002395"
     CDS             8877..9551
                     /locus_tag="PC541_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839854.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9554..10786
                     /locus_tag="PC541_002396"
     CDS             9554..10786
                     /locus_tag="PC541_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839855.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11072..12337)
                     /locus_tag="PC541_002397"
     CDS             complement(11072..12337)
                     /locus_tag="PC541_002397"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2839856.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12390..13538)
                     /locus_tag="PC541_002398"
     CDS             complement(12390..13538)
                     /locus_tag="PC541_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2839857.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13629..14174)
                     /locus_tag="PC541_002399"
     CDS             complement(13629..14174)
                     /locus_tag="PC541_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2839858.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14397..15341)
                     /locus_tag="PC541_002400"
     CDS             complement(14397..15341)
                     /locus_tag="PC541_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2839859.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         5401 a         2418 c         1948 g         5693 t
ORIGIN      
        1 atataaacac aggaggtaca ttctttgaaa aacattattt cagcaaaaga acttatatca
       61 aaactagaaa aaaatgataa tctagttgta atagattgta gatttgatct tatcaataga
      121 acttatggta ttgattctta taaaaaagga cacatcaaag gcagttttat cttagatata
      181 gacaaggatt tatcatctcc tacaaaagaa catggtggta aaaatccttt acaagaccct
      241 ttaatactaa aagaaaaatt agaaaaaatg ggtgttgata atgacactac gattgtaact
      301 tatgatgatg gagatttaaa tggagcttgc agattatttt tccaattaaa acatttgggg
      361 cttaaaaatg tctatgtact agatggggga ataacatctt tcgtaaagga aggtggagaa
      421 ttggaagaga aagtgaatat accaagttgt acaggtaagg aaattagacc aaatataaac
      481 aatgatttag ttgttccaat ggaatatgtt aaatctaaat tatacaaaaa agatactgta
      541 ataatagatt gccgtgctaa tgagagatac cagggtttag ttgaacctgc ttactcaaaa
      601 gctggtcata taccaagtgc aaaaaattat ttttgtaagg atttaataaa aagtgacttt
      661 gaaaatggct cacttaaaga tatagaattt ttaaaaaaat tctttaaaga tttaaataat
      721 tatgatgaag taattttatc ttgtggttca gggatatctg cttgtgttaa cagtcttgct
      781 cttagagaac tggatatacc tcacaagatt tatattggta gctttagtga ctggattagt
      841 tatgatgaca atgaaatcaa aactggtcaa gaataaggct cataatctat ataaaatcag
      901 catatgatag cttaaatttt gtaaaggcgt cagtcattgt agactttcgc ctttacttat
      961 tatttcatat actctttaaa tttattatat atattttagt tgataatatt aataaactgc
     1021 aatatgacca tcacatcttg attccgtccc acaaatataa ctttcctgtt ctgttttaaa
     1081 tattatttga cctcgtccca tgtcgacagt atcatgaatt acttttattt catgaccctt
     1141 ttcaattaat gaatctatta tatgctttgg catctctggt tcaacttcta tttcttttcc
     1201 ttttatccac tgccatcttg gagcatctag cgcctcttgt ggatttaatc caaaatcaat
     1261 catatttgtt aacacttgaa tatgtccttg tggttgcata aaagctccca ttacaccaaa
     1321 tggacctatt gctttatctt ctttacctaa aaatccaggt attatagtat gataaggttt
     1381 tttaaatggt tttactacat tgtggtgttt agggtctaaa ttaaaattat ttcctctact
     1441 atgtaaagca atcccagttc ttggtatcac aattcctgaa ccaaaattta tgtaattgct
     1501 ctgtatatat gaaaccatat ttccatcctt atctgcagta caaaggtata ctgtaccacc
     1561 acaatatggc tctcctgcag ttggatacaa agcctcttta ttgtcaatca ataaacttct
     1621 ttttttagca tactcttggc ttagtaattc ctgaatcttt accttcatat gctctatatc
     1681 agttacatat gttttagaat ctgcaaaagc taattttaaa ctctctataa ttttatgtat
     1741 gtcttctaca ttttctatat ttccttctaa ttccaattct tttaatatgt taagtgccat
     1801 taaaactgtt attccatgac catttggtgg gatttcaaat attttatatc ccttatattc
     1861 tgtacttata ggttctaccc atgttggata aaaacactct aagtcactct ttcttatagc
     1921 cccattaaat tttttagaat attcatctat tttatcagca agtcttcctc tataaaagct
     1981 ttcagcctga gtatttgcta tctcttctaa tgtacttgcc tgttcttcac atataaatat
     2041 atctccagcc tctggagctt taccatcttt agaaaatgta tcaaaccatg gtttgaactc
     2101 ttctccaata agttcttttt catagagttc atatgatttt ttccatacct tagctacatt
     2161 aggagatact acgtatccct ctcttgcata gtttatcgct ggagataagc attccattaa
     2221 gctaagttta ccatattttt tatttaattc tgaccaagct gctggtattc caggtacagt
     2281 tactgctcca aacccatatt ttggcatttc tttcatatta ttataatttt ttaattccat
     2341 attttctgga gcaaagccac tagaatttaa accatacaat cgtttttctt cctctatcca
     2401 aacaagggca tatgcgtctc ctccaatccc attgcttgta ggttctacta ctgctaatgt
     2461 ggcggctgtt gcaactgctg catcaacagc atttccaccc ttttttagta tttcaagtcc
     2521 agcttgagat gctaatggtg ttgatgttgc aactgctcca tttttagcat acacgacatt
     2581 tcttattgat gagtatttat gttggtaggc attaaacttc ataaatttca gctcctcgtc
     2641 attttttatt tgatattctg cttagattta ttatataaca tcaagtaatg taactgatac
     2701 aaaatgatat aaaatataga ctttttccta actttaattt tgtctttaaa taaatatctt
     2761 aaactcaatt ctagtatttt ctatctgcac ttaaataagt tattttattc aaaacatata
     2821 tctaattctt ttgacttaaa aataatttat tcttttatca tgtttagagc atattttaaa
     2881 gttaaaaaat aatatataaa atatcctata aaataaatga ctagagttat gataattggt
     2941 acaagtaatg accttggtat aaacttttgt gctatagaca tagcaaataa attatgaact
     3001 gtacctacaa ctaatggcat taaaaatata acttttaact gttttgatat tattttctca
     3061 atatctttat tatttgctcc aagttttatt aacataatat atctttcctt atcatcatat
     3121 atatttgata attgtttaaa taagattata ctacctgtac ataaaagaaa tactacagac
     3181 aaaaatatgc ctataaacag cattagacca cttaatttta tcaagttatt ataactttca
     3241 atacttgatg taaaattaaa aggataagta aatttcatat tttcacttac tatatttttt
     3301 aattttaaag tcaaatccag ttcttttttt tcatttttta tatcaattaa tcttattaat
     3361 ctagaatttc catgtaattt taactgatta aatacgtcat cttttgctac aattaaatca
     3421 aacgtagctt gtgaatttat ttctggctcc aagatgcttt tttgtacttt aaacagattt
     3481 atatcatact catttgtatc tttaatatgc aagtttattt tttcaacctc aaagtcttta
     3541 aaaaatattt ttttgtatga atcagaaaca tagtacacag attcctttga agttaattct
     3601 tcataattgt tattctgata ttgtcttaat tttttaaaat cactttctct aataatatct
     3661 acagacgttc tatcttctat ataactattt ccatttttat aatacatttc aatatttata
     3721 tctttgtcta taagttctat tgttttatca aagattatct tattatcttt tttatattta
     3781 tataataatt tgtcaatttc gttatttaca taagaatttc ctaaatttat ggtataactg
     3841 tatttataat tttcacttat gttcctatct atatcgtaat aaagtgatat ggtaaaccct
     3901 agcatagtta cacttgtagc tattaatatt gctattacag ccaatacact actattgctc
     3961 tttaatttaa atccaagttc agaaaatgca attaaattct gtcccttata ataaaaactt
     4021 tttctcttct taacagcatc aataatcata tacatcgttg atgaaaaaaa taaaaaagtt
     4081 ccaggtatta ttgtaactaa tataaaaact gaaagcataa tattttcagc aataaaataa
     4141 ctaattgaca ttaaataaga aatagctatt aataaaacac caagtacaca ttttaacttt
     4201 ataaattttt taaacttata cttttctgga tatttattaa ataactctat aattctctta
     4261 tttctgataa ctatagtatt tcttattcca ataactatga atattattaa aaataccgct
     4321 agtgtctgaa ctaaggcctt tatacttaag ttcatattta ctactatcat ttcattcatt
     4381 aattttacaa gcatcatagt cataagcttt gaaaataaca gtcctaaaaa tatacctatt
     4441 agtattgcaa ctatacccat caaaaaagtt tcatataaaa atatttttcc tatctgattt
     4501 ttcctcattc caagtaaact ataagttcct acttctcgcc tacgtctatt gaaaaaaaat
     4561 gagtttgaat aatatataaa taaaaataca aataccacta ttacaattga acttgtatta
     4621 atactcatcc tagttttctc tccaagtgaa ctcaacgctt cattatactc tatagattta
     4681 aaactaaaaa atacaaatac actaaataca attgaaataa agtacattaa ataattaaaa
     4741 aaactattct ttacattttg cattgcaatt ctaaataaag tcatttttat taactcctcc
     4801 aattaaggaa actgtattta ctattttatt atagaactct ctgttacttt ctccctttac
     4861 aatttctgtg aatattattc catcctttat aaagagtatt ctattgcaaa aactcgcact
     4921 tatagcatca tgagttacca tcaatattgt tttattgctg tctttgttta attttaatag
     4981 acaattcaac aaatccctag cagatttaga atctaatgct cctgtaggct catctgcaag
     5041 tactaatgat ggttttgttg ctatagctct acatacagct cctctttgct tttgtccacc
     5101 agaaacttcg ccagggtatt tatctaatat agattctata tttaattctt ttgaaatctc
     5161 ttttattcta ttttctatta ctgatactct ttcatttttt aaagaaagag gcaacattat
     5221 attttctttt attgtaagtg tatccaaaag attagaatct tgaaatacaa agcctagatt
     5281 atcacgtcta aaatctgcca attcattttg cttcatttta atagtattct ttccatttat
     5341 ataaatatct cctgatgaag gtaaatctat tgtagaaata atatttaaaa gtgttgattt
     5401 accagcaccg gaagacccca taataccaac aaattctcct ttataaacct tgaaagttat
     5461 tcctcttaga gcttcatgtt ttgaaccttt tcttccatac tcctttttta tattttcaac
     5521 agataatatt tcttccatag ttaataatca cctttcatca caattagtta tttatcaatt
     5581 ctcacaaata tattatatat attaaaatgg cttttaaaca tttaattata ttaatttaag
     5641 gttacatttt cgtaagtatt aattatattt cttcaaattt attgcaatta tgtttaaaat
     5701 ataatttatc aagtaaaata tagttatatt aatataatta ttttaggtaa taaggtagaa
     5761 atattttaat ttatcaagta gaatatagtt atgttaatat aactattttg aattaggagg
     5821 taagaatgtt taaaataatg gtagttgaag atgacgtctc attgaaaaat atcatagcaa
     5881 agtgtcttac taaatgggga catgatgttc atcaaataga aaatcttgaa aatataatag
     5941 aagagttcaa aaactataac ccagaattag ttttattgga cataaacctt ccattttatg
     6001 atggttttca ttggtgtaat gaaataagaa aaatttcaaa agttcctata atttttattt
     6061 cttcaagaaa ttctaatatg gatgtaataa tgggtgtaaa tttaggtgca gatgattata
     6121 tacaaaaacc tttttctgta gatgtcttag tagctaaagt taatgccctt ttaagaagaa
     6181 cttataattt tgttgataat aactcaaatc agattattca caatggtgtt actttagatt
     6241 tatcaactgc aacaataaac tatgaagata atactattga gctaacaaaa aatgaaatca
     6301 aaattcttca tgagcttatg aaatataagg ggcagatagt aagtaggaat aaactcatga
     6361 aaaaattatg ggataatgat tggtttgtag atgataatac attaactgtc aatgtaaata
     6421 gaatacgctc aaagttaaat gaaattggtt tagaagattt tatagaaaca aaaagaggat
     6481 tgggttatat aatcagttaa aacaattcta agtaggtgaa tttatgagct ttaaagattt
     6541 tctcaaggat aaactaggtt ttttagtata caattttact ttcttaattt ttacagtatc
     6601 agttattata ttttctcctg tagatgtttt tctgactgat actattttat atatattagt
     6661 tgtaaatgtt gtgtttttat ttttatatct atttattagc tacatcaaga agaacaaatt
     6721 tttagataag ataaaaaatg atacattcaa aattaatatg aatgatatag aattagctag
     6781 atgtaaaaat gaagaaaaaa tatacttgaa cattattaaa aatcatcaat atcaatgtaa
     6841 taacataata aataataata tcgagaaatt tgaagaaaac aaagagattt taaatatgtg
     6901 ggtacatgat gtaaagatac ctatagctat aataaaactc attcttgaac aaaatgaaaa
     6961 tttaattgat gaaaaaattt cagatgacat agatagtgaa atatttagaa ttgaaaactc
     7021 cgttgaaaaa attttatatt tatcaagact cgaagatttt cataaggatt ttttaattca
     7081 agaggtaaat attgaaaaaa tcattagaaa tattataaaa aaatactcaa aatattttat
     7141 ttcaaaaaaa attattttag acttaaataa tttgaatttt aaggttttat ctgatgaaaa
     7201 atggctatac ttcatattcg agcaattatt gagtaattcc ctaaaatact cctcattagg
     7261 agataatata tctatctatt gcaaaagaac caaaaattat ttacaattaa gagtggaaga
     7321 tactggttgt ggtataaaaa aggaagattt aaatcgaata ttcaataagg gatttacagg
     7381 taataatgga agaaacaatt ctaaatctac aggtcttggg ctttatttag taaaagaact
     7441 ttgtgataag cttggtcata aaattgatgt agattcagag tacaaccaat atacaaaatt
     7501 tactattaca ttcaaatgtg atttatagta tcttattatt gatagtttat tttcattaac
     7561 ttatattcaa cttcttatag taattctata tgtacatccc agtatataat taagtagttt
     7621 ttataaaaag gaggaagaca tgtcttttct aaaaaaattt aaaaataaaa gatttagaaa
     7681 taacagtttt aaaagtaaca actttagaaa tagcagtttt ggaagtagtg gcttcaagtc
     7741 taaaaataaa ggttttaaaa acaatccctt taaaaataag agattcaaaa tatatgcagg
     7801 aatagcagta atcatacttt taattctagg agttcttgct tatgttgatt cgaaaaacac
     7861 tgaattacaa gctaatgaaa attttatcga aacatatact attcctgaaa atgaaaaaat
     7921 atttataaat gggatggttg ttcctaagca aactaaggac ttcaatatat ctggagatta
     7981 tgagttaagt gatgttaatg ttacaaatgg tcaaaaagta aatcaaggag accttctttt
     8041 tactgctaaa aatccaacta taatagcaga aatagatagt ttaaaatcac aacttagtca
     8101 atacaaaaaa caaaaaatat cactttctga tattgctgaa aataaagatg ctattgcttc
     8161 tataaatgca cagataactg ctttaagctc ccagatagct tctctggata aaaaagctta
     8221 tgacagagtt actgctcctt ttgatggcac agtatacttg aatgaccaaa ctggaaatcc
     8281 agaccaacca gtttcattta tgactattca aggtctagag ttttatatga aaggacaagc
     8341 aagtgaacaa gatttaccta aaatgaagat tgaccaaatg gtcaatatac tagttttttc
     8401 aactgaccaa aagcttactg gtagaataag ttttatatct gataaaccat ctactccaaa
     8461 tactgaaatg ggagctcagc agaatacact atcttattat gatataaaca tagcatttga
     8521 taatcaagaa ggattagtaa atggattcca tcttcaagca tcacttgaag tagctaattc
     8581 aagctttaaa ataccagcat cttgtgtact taaagacaac aaacactcat atgtatttaa
     8641 ggatttagat ggtatattga aaaagcaaat tgtagatgta gcaagtcaaa atgatgattt
     8701 tgctgtagtt agaggcggtc ttgaacaagg cgatataata ataaaacacc ctactaaaga
     8761 aatgaaagaa ggagacccag ttcaaggcga tggcgtaact gctggttcta ataatggaga
     8821 tactactcca aacaaaaaag tagaagaagt tactatggat gtgaattaat ttacatatgt
     8881 taattaaatt agaaaatatt caaaaatatt acaaagtagg gaaagatgaa ttacatgttt
     8941 taaaatcctt aaatttagaa attgaatctg gagaatttgt catgataatg ggtaagtctg
     9001 gtagtggtaa aactacttta cttaatatct taggttttct agatgtattt gatgaaggaa
     9061 gatatatatt tgatggaaca gatgtaacta atttgagtga aaatgaacgt tctgttttta
     9121 gaaatattaa tattggattt gttttccaac aatttaattt aatagaaact ctaaatgtat
     9181 atcaaaatgt agagttgcct ctaatttaca ataaagcact aaaaaaatct aatagagaag
     9241 aaattgttaa agataaatta agttctgttg ggcttttgga caagcttaag cagaaaccac
     9301 ttcaattatc tggtggtcaa caacagcgtg tagccattgc acgttgccta gcaaatgacc
     9361 ctcagataat ctttgctgac gaaccgactg gggcattaga tagtgaaact agcagagaaa
     9421 ttatggagct tttaacaagg ctcaacaaac aaggtaagac tataataatg gttacacatg
     9481 atcaagattt aactaagtat gcaactaagg ttatacgact taaagatggt gtgtttactt
     9541 cggaggtgta atcatgagtt tattaaaaaa ttctttggcc aatttaaaag gtcacaagct
     9601 tcgtgtattt gtagctttac tctggattat aataggaata acttcagtaa tacttgttag
     9661 ttctataggt aatggatttc aaaaagaaat taagaaatct gtaaacaatg taaaccctaa
     9721 taaaactact atttcgtttg aatcagccga taacacaggt cttactgatg atatgagtat
     9781 ttttttaaaa ccatttaatg caaaagattt agaagaatta tcatttgttg aaggagtaga
     9841 aagaatagct ccttcaagag atgggtttaa ccttgattca gtttactctt cacaagcatc
     9901 ttttgataaa aaaactacgt atgttgatgt tggaccagta aaaaaagatt ctaaaataaa
     9961 tttaatttgt ggtagagatt tttctttaga tgatgaaaaa agaaaagtaa ttttacttac
    10021 attacaaagt acaagtgaaa tttttgaaaa tccagaagat gccttaggtc atggtatcaa
    10081 cataaatgga actatatttg aaataatagg ggtattagat gattctcagc aaaaccaagc
    10141 tggaggattc tttggaggtt accaagatat gcaatttact acttccctag tacctaaaaa
    10201 ggcttttgac actttaatga gtcaaaattc ttattcaaat gaaatttacc agttagactt
    10261 agtttcttct aaaggctata atgtaaatga agttgcaaac aatgttatag ctaaattata
    10321 tgagatgcac cctggtatca atggttctta tactacacca gacccaactg aacagacagc
    10381 ttatcttgaa agcattaatt ccaatgtaaa caaatatgta tctataataa ctgtagttgc
    10441 aatgtttgtt ggtggtattg gtgtaatgaa tataatgtat gtatctgtta tggaaagaca
    10501 aagagaaatt ggtataagac gtgctattgg agcaaaacct aggtctatat tatttcaatt
    10561 tttagttgaa gctgttttta taacagtatg tggtggaatt ttaggaacta tagttggatt
    10621 tactgctact aactatgttt ctaaatatat aggttttgaa gctataccta gcttaaatag
    10681 cttattttat gcaattgtag ctactatatt gactggagtt gtatttggtc tgattccagc
    10741 ctttaaagcc tctaaactag accctataaa ggcaatttat aagtagaaat tgtataaaga
    10801 ttttcgattt ttagattagt ttatataaaa catacttaaa tatataacgc actattatat
    10861 atgatagatt aggaacttaa tctttccaaa tataatagtg cgttctttca taaaaaggat
    10921 tctcttcctt actatatcta aattaccaag atgttatgtt ctaattgttc aaactaatac
    10981 ttccaatttt gattaatcaa tctcattctc ctaatataca atcttttact ttcaatcatt
    11041 gtacactccg cctaaaaaaa ataatcttaa attaggcgtt atatacaatc tccccattac
    11101 atattgtata attaaccttt ccatataaag atttaccttt aaatggtgaa ttatcagctt
    11161 tagaaacaaa gctttcaact gtccattctt cttctggatt gaatataaca atgtctgcta
    11221 cagccccttc ttctataaat ccaatattta aattgtacaa ctttgcagga tttatagaca
    11281 ttttttctat taactgcatc atactaagat gatttttatg caccaaattt gttattccaa
    11341 gagctaaaga agtttcaagc ccaataattc cacttggagc ttttataaac tctctatctt
    11401 tttcttcttt actatgaggt gcatggtccg ttgctattat ttcaatagta ccatcattta
    11461 atccctctat aatcttatct ctgtcccatt tacttcttag aggtggattc atttttgcat
    11521 ttgttccaaa ctccaataca tcttcctcag tcaatgtaaa gtgatgtggt gaagcttctg
    11581 caactacatt tgcacctaag tattttgcaa atctaaccat atcaacagat acacctgaac
    11641 ttatatgttg tatatcaact ttagcaccag tctttagtgc caacatacaa tctctggcta
    11701 ccataacatc ttctgctaca cttgatgcac cttttaatcc aagttctgaa gctacctttc
    11761 ctgcatttac acctggattt ccaactaaag aagggtcttc ttcatgaaaa ctaagtggta
    11821 catccaactc ttttgcctta atcattgcct ccataattag attgctatcc attaaaggta
    11881 atccatcatc agtaaaccct ggtacaccag cttttttaag agcttccata tcaactaact
    11941 ctttaccttc aaaaccttta gttatagctg ctgcttgtaa aacatttata catgcatttt
    12001 ttgacttttc tttaacataa ttaaaagtat cttcattatc tacaatagga ttagtatttg
    12061 ccatacatat aacagttgta aatccacctc tagcagcaga tttagctcca ctttctatat
    12121 cttctttata tgtaaaccca gggtctctaa agtgtacatg tacatccact aaacctggag
    12181 caactacatt accactagca tctattattt tttcatattc atcactttca tcaaacttgc
    12241 ctattttata aattttattt tcttttataa ttaagtcaat attctcatct ctttgacttt
    12301 tagggtctat tactctacca tttttaatta aaatcatatt atttctcctt tatattttta
    12361 atttatatag actcatataa tattatacat tattttacat acttaaatac aaaattacgt
    12421 ttgacatcaa tagccttttt aggacaaagt tcatggcaac agtaacatct tatacattta
    12481 cttaagtcta taatagcacc cttagtccct atctctatag tggatgcagg acaagcttcc
    12541 ttgcattttc cacattttat gcaatcttga aatcttacca caggctttgg tgttataagc
    12601 tttgaaatag gttcatttaa aaattttggt agctttacag aaaacaatct taaatcctta
    12661 cttacatttg gaatcttgta attttttata attaaatctt ttatatcttc acctaaaatt
    12721 tcaatatctg aaaaatcttc ttgtatatat cctctcttaa tagcacctct aattgtagca
    12781 actctatttg gaagtaaact tataatctta catgcaacaa catctatagc atatggattt
    12841 tgtgatgcta ataaaacgcc tatttctcga gtagtaccag ctgaaggtcc atcaccttcc
    12901 atacctacta taccatccat aattgtcaat gttggtttta tataatcaca aatatctaaa
    12961 agtacatttt cgcaaaacaa ctcttctgta ggaaatctat aatgtatctc tgcttttttc
    13021 aatccagcta tgcatccata taagtttttt gtaccaccag taaaagttgc cattatatga
    13081 gtctttaatt tacaaagatt tattatataa tctgaatcta ctattggttt aataatatcc
    13141 atatatttta gaacatgtgc tttttcacta tgtatcttaa catctgaaac atcataattt
    13201 aacttaatgc ccagttcatt tgctacttct tcaattccag atgccttata aatgctctta
    13261 agagaacttt gattatatgg accaccaggg ctatctgcta ttgtaatatc acaatttaac
    13321 tctaaaagtt tttcacatac aacttttaca accataggat gtgtagttgt agcttcttca
    13381 ggcttttttt tcattaataa atttggtttt ataagtactt tgcttccttt tttaacaaac
    13441 ttatctatac caccaatatc ctctaaattt ttttcaatag ataactttac ttcttcataa
    13501 ttgtaagttt tacaactcct aacagatacc gacttcatat tatctccatc cttctatcac
    13561 aacagtttat ttgtataatt taaaaaagga gaaaccaaag tttctccttt taatctatgt
    13621 tttaattatt attttccaaa atgtctgttt aataatccta agaatgcttt accatgtcta
    13681 gcttcgtctt tacacatttc atgtactgta tcatgtatag catctaatcc taattcttta
    13741 gctctcttag ctaattttaa ttttccatca gttgcaccat actcagcatc aactctaact
    13801 cttaagtttt cttttgtatc tgcaactaca acttctccaa gaagttctgc aaatttagca
    13861 gcatgttcag cctcttcaaa agctattctc ttatacgctt cagctacttc tggataacct
    13921 tctctatcag cttgtctact cattgctaaa tacattccta cttctgtaca ctcaccagta
    13981 aagttggctc ttaatccttc gattatttct gcatctacac cttgagctac tcctattcta
    14041 tgttcatcag cccaaaccat ttcgcctttc atttcttcaa atttatcagc tccaacttta
    14101 catactggac attgtgctgg tgcagcatct ccttcatgta tatatccaca tactgtacaa
    14161 acaaattttt tcataattaa tttcctccca attttatata attaaatttt tatttttaag
    14221 tttaataact tgctacaaga atatatatac cccagacttt atagcttaaa cactaaaaat
    14281 aaaatttatt tcctaattaa ttttatcact ttgaaacacc agtatcaacc aagttttgat
    14341 gtcaaatttc tacataatac ataatttatt tgattaatta ggaaatagtt gtcatattaa
    14401 atactttcta atgaagactc ctctgaatct ttaagtttca cttttttgct ctttaaaaaa
    14461 gataatttag tttctgcagt tatgattgca acaaatatca agatactacc aataaccaat
    14521 tttgctgtaa taagctcctt taaaattatt atagaaaata ttgtaccaaa tacagcctct
    14581 gtggataata ttatagctgt tctagttcca tcaactcttt tttgacatat agtctgaaat
    14641 aaaaatccaa ttgtagtact aaatacagct agatacatag ttcccatgta accagataat
    14701 tcagcttcca tctttaattg acctgcaaat gtttgaacta caacagacat taaaaacgct
    14761 actgtaaatt gaatagctgt caatgccatt gggttgttat cttttgcaaa ctcacttgta
    14821 aagaatatat gaaatgcaaa tccgaatgaa catataagtg ttagaaaatc tccaaaatta
    14881 atagagaaat cagcttctaa agaaagtatc ccaattccta taagagctac caaactactt
    14941 attattccta ttttatcaag ttttcttcta taaagtataa atcctatgaa tggaactatt
    15001 acaacattag ctgctgttat aaacgcattt ttagatggtg tagtgtacat aagtccaata
    15061 gtttgtactg tgaatgctag aaataaaaat attcctaaaa tccctcctgc ttttaataat
    15121 tttttaccca tattagcacg aatctgttta aaaaacagta aattcataat tatagctgcc
    15181 aagaaaaatc ttaaagttat gatttgtaga ggtgtaagcc caccgtctaa tgcaaattgg
    15241 gttgctacaa agccacttcc ccatataatt gctataaata ttagtcctat ttctccaaag
    15301 tattttttca ataaagcccc ctccttttta tttttttgca aaattataaa ccccctaaac
    15361 agcaagagag tttttctata agcattacta taaattaata tagtaaattt tttcagtatt
    15421 ttaaatctaa atttacatac attttgagct caaatttata
//
LOCUS       DAMJZE010000094        15451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJZE010000094 DAMJZE010000000
VERSION     DAMJZE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.095"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            197..754
                     /gene="efp"
                     /locus_tag="PC541_002401"
     CDS             197..754
                     /gene="efp"
                     /locus_tag="PC541_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2839860.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            846..1301
                     /locus_tag="PC541_002402"
     CDS             846..1301
                     /locus_tag="PC541_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2839861.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            1464..2174
                     /locus_tag="PC541_002403"
     CDS             1464..2174
                     /locus_tag="PC541_002403"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2839862.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            2228..3298
                     /locus_tag="PC541_002404"
     CDS             2228..3298
                     /locus_tag="PC541_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2839863.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            3326..6880
                     /gene="smc"
                     /locus_tag="PC541_002405"
     CDS             3326..6880
                     /gene="smc"
                     /locus_tag="PC541_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2839864.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            6907..8187
                     /gene="ftsY"
                     /locus_tag="PC541_002406"
     CDS             6907..8187
                     /gene="ftsY"
                     /locus_tag="PC541_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2839865.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            8290..8658
                     /locus_tag="PC541_002407"
     CDS             8290..8658
                     /locus_tag="PC541_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2839866.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            8655..10007
                     /gene="ffh"
                     /locus_tag="PC541_002408"
     CDS             8655..10007
                     /gene="ffh"
                     /locus_tag="PC541_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2839867.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            10045..10317
                     /gene="rpsP"
                     /locus_tag="PC541_002409"
     CDS             10045..10317
                     /gene="rpsP"
                     /locus_tag="PC541_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2839868.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            10382..10609
                     /locus_tag="PC541_002410"
     CDS             10382..10609
                     /locus_tag="PC541_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2839869.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            10717..11232
                     /gene="rimM"
                     /locus_tag="PC541_002411"
     CDS             10717..11232
                     /gene="rimM"
                     /locus_tag="PC541_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2839870.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            11241..11936
                     /gene="trmD"
                     /locus_tag="PC541_002412"
     CDS             11241..11936
                     /gene="trmD"
                     /locus_tag="PC541_002412"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2839871.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            12121..12471
                     /gene="rplS"
                     /locus_tag="PC541_002413"
     CDS             12121..12471
                     /gene="rplS"
                     /locus_tag="PC541_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2839872.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            12792..13688
                     /gene="ylqF"
                     /locus_tag="PC541_002414"
     CDS             12792..13688
                     /gene="ylqF"
                     /locus_tag="PC541_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2839873.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            14077..15369
                     /gene="brnQ"
                     /locus_tag="PC541_002415"
     CDS             14077..15369
                     /gene="brnQ"
                     /locus_tag="PC541_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2839874.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         6546 a         1481 c         2946 g         4478 t
ORIGIN      
        1 aggataataa taaaaatata gaataagtaa tttgccaaat acttgtttat attgtataac
       61 ttatgatatt atgatattag taaaaatttg taatcctgac ttgacagaat tcaagtaaga
      121 gatataattt taaaggcata gtaatgtaaa atttactaaa aaataagaaa tttattttaa
      181 tatggaggaa caaaaaatgg tatcagcagg tgattttaga aaaggtgtta catttgaaaa
      241 agatggacaa ccatgtttag tagttgattt tcaacacgtt aagccaggta aaggagctgc
      301 tttcgttaga actaaataca aaaacttaaa aacaggagct ataagagaag aaagtttcaa
      361 tccaagtgaa aaattcccta aagctgttat agatacaaga caaatgcaat atctatacaa
      421 tgatggtgag ttatattatt ttatggatca agaaaatttt gaacaaatac cattaaacta
      481 tgagcaagtt gaagatgcaa ttaagttctt aaaagaaaat gaagttgcta caataagatt
      541 ttaccaagga caaccattcc aagtggaagc gccaaacttt gcagagctag aagttacaga
      601 tacagagcca ggtataaaag gtgatacagc aagtaatgta actaaagcag ctacagttga
      661 aactggagca gttgttcaag ttccattatt tataaatact ggagataaag taaagataga
      721 tactagaaca ggtgaatatt tatcaagagt ataaaaattt atgtatataa aagcttatat
      781 gtcttaataa tataagatat atgagctttt agtttatata taaaaaagaa agcgaggggt
      841 gttacatgaa acatttatat gaagaagttg catacttaaa aggcctagca gaaggactag
      901 aaataagtgc tgaaagtaaa gaagggaaaa tgatacataa aatagttgat gcattagaag
      961 tatttgcaga tgctattgta acattggatg aagaacaaga agaacttcaa gattttgttg
     1021 aatctataga tgaagattta gcagatttag aagaagatat tgctgacatg gaagaagatc
     1081 tttatgaaga agatgacgat gaggatgatg aggacttcag ctatatagaa atggaatgcc
     1141 caaattgtgg agagttagta gaaatagacg aagatttgtt atatgatgat gaagtagatg
     1201 ttgtttgtcc tgattgtaag gctgtgatat tatcatcaga agatgattgt gatgatgatt
     1261 gtacatgtgg tggttgttca agttgtgatg atagagagta aaaattcata agaattaaaa
     1321 ctgcctatga tgcctatgag atattttgct tcataggtag ttttattttt atgaggaagg
     1381 gtattatgta ttttggtttt gcaatacttg gacaaaaata gtacactaga atttatagaa
     1441 taggattttg gagggatgta aatatgaaaa taagtaaaaa attactagat aatatacaaa
     1501 gatttgaaaa tgttataaat tataaattta aaaataaaga atatatacta gaagctctta
     1561 ctcatagttc atattctaat gaaaataaaa aatataattt taacgaaaga ttggaatttt
     1621 taggggattc ggttttaggc atagtgataa gtgattattt atttaatgaa gaagctaatc
     1681 tgcccgaagg ggaactaact aaacttagag caaatatagt ttgtgaagat tcattaagtg
     1741 aagtagctaa tgatataaat ttaggtattc acatgttatt aggtagagga gaagaagcaa
     1801 caggaggaag acataggact tcaatattag cagacgcatt tgaagctgta atagctgcta
     1861 tatatcttga tggaggtttt gaaagtgcaa gacagtttat acttcatcat atggaaaata
     1921 taatttatga ttcaagaaaa ggaaatatat ttagagacta caaaacgcat cttcaagagg
     1981 tgttacaagg gaatggagaa aataatatct ggtacaggct tatagaagaa aaaggaccag
     2041 accataataa aagatttgta atggaagttg gaattaatga cgatgtactt ggtataggag
     2101 aaggcaagag taaaaaagaa gctgaacagt tagctgctaa aatagcttta aagaaaaaat
     2161 tatgggaaaa ataaatataa tatgtaaaaa tataaaaaga aatatttata ttagaaggat
     2221 gttgtagatg aaaaagagaa ttatacctat atttgttcct cacaaaggat gtcctcatga
     2281 ttgcatattt tgtaatcaaa aaaaaataac aggtgtgagt actgatgtta ctagtgaaga
     2341 tgcaagaaat ataattgagg aatgtttaga gactatagat aaagatgcag atgtagaaat
     2401 agcatttttt ggtggtagct ttactgctat agatattgat atacaaaaaa gtttattatc
     2461 agttgctaaa gaatatgtag aaaaaggtct tgtaaaagat attagaatgt ctacaagacc
     2521 agattgtatt gatgaagata tattaagtat gttaaaagaa tataagacaa gtataattga
     2581 actaggagta caatccttag atgaaaaagt attattagat agtataagag gtcatcaatc
     2641 tgaaattgta tacaagagtt caaagatgat aaaaaatagt ggaatcaaac ttggattaca
     2701 gatgatgatt ggtcttccag ctgacacaga agaaaagtgt atatttacag caaaaaaatt
     2761 tgtagagcta aaaccagatt gtgttagagt ctatccaact ttggtagtaa aagatacagg
     2821 tcttgaaaag ttaatggaac aaaataaata taatccattt actcttgaag agagtgtaaa
     2881 tatagtaaaa aaagttcttg tactgtttta tgttaataat atcaatgtaa taagagttgg
     2941 gcttcaagct actgatgata tacaaattgg aaaagctgtt ttagcaggac catatcatcc
     3001 tgcatttaga gagttggtag aagcagatat gataaaagat tatttagaat ttgttatttt
     3061 gcaaaataaa aatataaagc aaatgttggt caaatctaat aaaaagaata tttctaaaat
     3121 cataggaaat aaaaaaacaa atgttaaata tatggaagaa aaatttggtg tgcttttaaa
     3181 aacacaagaa tcagatttag atataaatca attagaaatt gtgttagatg ggaaaagttt
     3241 aattagtgct aatatgagag acattcatag aaaattgtat gacatttatg acctttagtt
     3301 ttaataatga agggagagaa aagatttgta tttaaaaaga ctagagttaa agggatttaa
     3361 atcctttcca gtaaaaacag atattatttt taaagaagga ataacagcca tagtaggacc
     3421 aaatgggagt ggaaaaagta atatatctga tgctgtaaga tgggtattag gagaacagag
     3481 tattaagagt cttagaggtg ataaactcga agatgtaata tttgcaggta cagatacaaa
     3541 aaaacctatg aattattgtg aagtggctct tactattgat aattctgaaa atcaattgga
     3601 attagatttt acagaagtaa ctataagaag aagggcttat agaaatggtg aaagtgaatt
     3661 ttttctaaat aataaaagtt gtagattaaa agatataaaa gaagtctttt tagatactgg
     3721 tattggaaaa gatggttatt caatcataga acaaggaaaa gtagatgaaa tattaagtaa
     3781 taatccttta agcagaagaa aagtttttga tgaagcttgt ggcatatcaa aatatagata
     3841 taaaaaacag gaagcagaaa ggaatttaag taatacaaag gaaaatttag aaagaataga
     3901 tgatgtatat atagaaattg aaaatcaatt aaagcctctt tttaatcaac agacaaaggc
     3961 aaaaaagtac ttggaaataa gtgaaaaatt aaagacactt gaagtaaata gctttataag
     4021 agaaattgaa ggaatagaaa aagaattaag tgaggttaat gaacatcgta aggttattga
     4081 aaaggagctc aatgaaaaag aagaacaaaa aaatgttgta gagaaaaagc aagaagatat
     4141 taataaagag gtagaggtat tacaagatgt aattgaaaaa tctgtagatt atataaattc
     4201 aattaaagga gttatatcaa aaaaagagtc ccaaataaat ttaattaagg aaagaataag
     4261 aaattttacg aatgaaatta gtagaaaaaa tttagaaata aaggatataa aagagaagct
     4321 aaatgaaaat aagcaataca taaaagaatt agaaagcaat aaattatcag gtagtgaaga
     4381 actatctacc ttgcaggaaa atataaaagt attggaagga agtaaagata aacaaaaaat
     4441 taagttagaa tccctaaata atgagataga attattgaaa gaaagtataa ttgatatatt
     4501 aaataagaaa caagagttta gtaataagct gtctacttta aacgcaaata aagaaaatat
     4561 gaatatcaga gatgaaaata taaattcaga gataacagag ttaaacaaaa atattgaaat
     4621 aaaatcatct gaattagata ctataaataa agaatttaat atgcaaaatg aaaatttaaa
     4681 gaatgtaaat aatagacata aagaattatc aattaatttg caggattcaa ttagtgaaca
     4741 caataaacta gaagatgaaa tacagaaaag taaatataat ttaaatggat ataattcaaa
     4801 gttaaatgtc tatatagata tggaaaatca ttatgaagga tttaatagag gagtaaaaga
     4861 agtactaaaa aataaaaatt taaaaggagt tcatggagca ttaggacaga ttataaatgt
     4921 acctgaaaag tatgagaaat ctatagaagc agctcttggt gcttatatgc aaaatataat
     4981 aactgataat gagtttagtg ctaagtttgc aataaactat ctaaaacaga ataatttagg
     5041 tagagtaaca tttcttccat taaatataat taaatcaaat aaaataagtc taggaaattt
     5101 aaaagcaaat actaagttca taggaatagc aagtgattta attacatttg atgaaaagta
     5161 tagaaatata attgaaaata tacttggaag aacaatactt ataaataata tagatgaggg
     5221 cataaaattt gcaaaagaaa ctggtcatag gtttaaaata gtaactttag atggtgaaat
     5281 cttaaatcca ggaggttctt tgactggggg aagtctaaaa actaatggga atatactatc
     5341 gagaaaaaga tacattaatg aatatacaga aaagataagt aatatcaaga atgaaatttc
     5401 acatctagaa ttaaaaagag aatctttaga caaagatgtg aaaaatatca aaaatgaaat
     5461 tgattcacat gaaagtaaaa taaaagattt agaaaaaagt ataataataa aaagtacaag
     5521 tataaagaat gttgagtctg aaattgaatc gttaaaaggt agtattacta aattagaaaa
     5581 tgagaagaat gatttaaatt ctaacctaaa ttatacactt gaaaaaagtg atgatgttag
     5641 aaaagatatg gaagagttag atgatttata caataaaaat aaagaaaaaa ttgatgcatt
     5701 gaatgaggaa ataaaaagat acaatgattt atatgataaa gaaaaatctg aatttgatga
     5761 attgaactta agtttagtga agaaaacaga agtttacaat agtatagtta gagatataaa
     5821 aagaatatct ggtgaaaatt gtgagctaga agaaaaaaat aaacaattag aagagtcatt
     5881 aaattatgaa gaacatgaaa taattaaatt acaagattct atactcactg aagaaaaaga
     5941 aaaagaaaat ttaacaaaac aactagggga tagtaataga aatcttgaaa ctagaaaaat
     6001 agcaaaagat gatttaaaaa atagttttga tgaaattaat aaagaattaa aaactataga
     6061 tagacagcat atagaactta aagaaagttt atttaaagtt ggtggaagat tagaaagatt
     6121 aaaaactagt caagacacat atataaataa gttgtttgag caatatgata tgaccttagt
     6181 acaagcattg gaaattaaag atgaagattt ggatatagat agaaaattct tagaaagtct
     6241 aaagagagaa ataagaagtc ttggaaatat aaatatagac tctataaagg aatatgagga
     6301 aataaaagaa agatacgatt tttatagcga acaaaagcaa gatttagaag agtctatgga
     6361 agagatagaa aaacttatac atactttaga agaaaatatg aaatctgaat ttgaaattaa
     6421 gtttgaagaa ataagtaaaa attttaaata tgtatataag agattatttg gtggaggttg
     6481 tggagaatta actattttag ataaagaaaa tttattagaa agtgatatac taataacagc
     6541 tcaaccccca ggtaaaaaaa tgaaaaattt aaacctatta tctggtggag agaaagcttt
     6601 aacggcaata agtatattat ttgctatatt gattacaaag ccaacaccat tttgtatatt
     6661 agatgagatt gaagcgccac ttgatgacgc aaatatattt agatttggag aatttttgaa
     6721 ggatttatct aaagagactc aatttatatc agtaactcat aggagaggaa caatggaagc
     6781 tgctgattat atctacggag ttacaatgca agaaaaagcc atttcaaaag ttattagttt
     6841 aaaattaaaa gaagctcagg aaataacaga tattatataa cttagattga cataggagga
     6901 ataacagtgt taaaaaaatt atttggtttt ggaaaagaca aggaaaagga aatagaaaaa
     6961 aaagatgctg aagaagaaat agaagttgaa gacagtgttg ataatttaga aaacttggaa
     7021 gaaactattt tttcaggact tgaagaagaa gtgatagata aagtggaaga tattgaagaa
     7081 aaaaatgagg aaagtgtcaa taatgaaggc actgaagaaa tcgaaaactt tgaaaaaata
     7141 gaagttgaga tggatagtaa tggaaaagaa gtagaaaata tttcaaataa tgattctcaa
     7201 gaagagataa tcgaagaatt agaaaattat gatgaaattg agtctaaaga gatagaagat
     7261 aaagaagata agaaagttaa cttgtttgaa agactaaaac aaggattgac aaaagcaaag
     7321 caaggaataa ctgatagaat agatgaagtt ttaaaatcat atacaaaaat agatgaagaa
     7381 ttacttgaag atttagagga aattttaata acagcagatg ttggtgtaaa tacaactatg
     7441 gatataattg aaagactaag agataaaata aagcaaaaag gtattactga accaataaag
     7501 gtaagagaag aacttaaatc tatagttgaa gatattctta caaatgaaaa ctctacacta
     7561 gatatagaac cagcaccatg tataatatta atggtgggtg taaatggggt cggtaagact
     7621 actacaatag gtaaactagc aaatagatac aaaaaagatg gtaaaaaagt tttattagca
     7681 gcagcagata catttagagc agcagcgaca gagcaattag aaatttgggc aaatagaacc
     7741 aatgtagaca taataaaaca tcaagaggga gcagaccctg gagctgtagt atttgatgct
     7801 ataaaagctg caaaagctag aaaaacagat gtattaatat gtgatacagc aggaagatta
     7861 cacaataagg ctaatcttat gaatgaactt ggaaaagtat ttaaaatagt agatagagaa
     7921 tttccagaag caaaacgtga agtacttctt gtggtggatg ctacaacagg tcaaaatgca
     7981 gttgttcaag caaagacatt taaagaagtt gcagatataa caggtatagt acttacaaaa
     8041 cttgatggaa ctgcaaaagg tggagtagtc cttgcagtaa aatcagaagt agatgtacct
     8101 gtaaaactta ttggagttgg agaaagtgta gaagacctac aagatttcaa tgcaaaatca
     8161 ttttcagatg cattatttgg taactaatgt tcttttaaaa ataatggttg acaagagtgt
     8221 tgttatgata taaaatacaa tagtgtaaag taaataacct ttacagtata ctgctaggaa
     8281 gtgtaagata tgaatataga aaaaatggtc gaaattggat tgttatttga acagtataaa
     8341 gaattattaa ctgacaagca gaaagaaata gttgctctat actatgaaga agattattct
     8401 ttaggtgaaa ttagtgaaaa tctgaatgta tcaagacaag gtgtttatga taccttaaag
     8461 cgttcagaaa aaatactaag agattatgaa gaaaagttac acttagtatc aaagattcaa
     8521 gagcaggaaa aaaatattaa gattataaaa gataaaatta ttgacattaa agaagattta
     8581 ttgcacaata gagattgtgc taatttaatc cctaagctag aaaatataga agatgtatgt
     8641 agggagatga taaaatgata tttgaaggat tgtctgataa actacaaggg gcatttagta
     8701 agttaaaatc taaaggaaaa cttacagaag tagatgtaaa aaatgctatg agagaagtta
     8761 aacttgctct tcttgaggca gatgttaact ttaaagtagt taaagatttc ataaaaaaag
     8821 ttcaagagag atgtgttggg caagaagtta tgcaaagctt aactccagct cagcatgtaa
     8881 tcaaaattgt aaatgaagag cttacaagtt taatgggaga tgtacaaagt aaagtaatga
     8941 tttcctcaaa gcctcctaca attttaatga tggttggatt gcaaggtgca ggtaagacta
     9001 ctacagcagg aaaacttgga ggatatttta aaaagcaagg taaaaaacct ctattagtag
     9061 cttgtgatat ttatagacca gcagctataa agcaacttca agttgttgga gaaaaattgg
     9121 atatacctgt attcaatatg ggagataaag aaaatccaat aaatattgcg aaagcaggtt
     9181 taagccatgc gattaaaaat gctaatgatt tagttattat agatacagct ggtagacttc
     9241 atatagatga agtacttatg gatgagttaa agtctattaa atcagaagtt aagccacatg
     9301 agatactttt ggttgtagat tctatgacag gtcaagatgc tgtaaatgtt gcagaaagct
     9361 tcaatgaggc acttggtgtt gatggagtag tattaactaa attggatgga gatactagag
     9421 gtggtgctgc gctttcaata agagcagtta cacaaaagcc tataaaattt atggggatgg
     9481 gagaaaagct agatgatata gaacctttcc acccagatag aatggcatca agaatattag
     9541 gtatgggaga tgtcttaagt ttaatagaaa aagcacaaga aaatatagac ttagagaaag
     9601 ctaaagaatt agagcaaaaa attaaaaaac aagagcttga ttttgaagat ttcttacaac
     9661 aaatggaaca aatacaaaat atgggacctc ttgacaagat acttggtatg atacctggta
     9721 tgggaaatgt aaaagaccaa cttggagatg tagatttgaa tggcaaagaa atgaagagaa
     9781 caaaagccat aattcagtca atgactacat atgagagaag aaacccagga gtattaaatg
     9841 cttcaagaaa gaaaagaata gctaaaggca gtggaactag tgtacaagat gtaaatagac
     9901 ttataaaaca acttaatgaa atgaaaaaaa tgatgaagat gtttgctggt tcacaaaaga
     9961 gtatgaagaa aaggggcggc ttaggcggtt tacctttttt taaataaaat ataaaaaagt
    10021 aaaaattatt cggaggtgac tgtaatggca gttaaaataa gattaaaaag aatgggagct
    10081 aacaaaaaac ctttctacag aatagtagta gctgattcaa gagctccaag agatggaaaa
    10141 tttatagaag aaataggata ctataatcca atttctgaac ctaaacaagt aagaataaat
    10201 gatgaaaaag ctataaaatg gttagctact ggtgctcagc caacagaagt agttaaaaaa
    10261 ttacttgtaa aaaacggagt aatagaaaaa ttcgaagctt ctaaacaagc aaaataattt
    10321 tttcttggca attagtttaa gtaagggctc attgctaagg ttactttagg agatgaaata
    10381 tatgaaagag ctagttgtag atatagctaa ggctctagtt gataatcctg actcagttgt
    10441 tgttgaggaa tttgaagata atgatggtat cgtcttaaag cttacagttg ctcaagagga
    10501 tatgggtaag gttattggga agcagggaag aatagctaaa gctataagaa ctgtcgtaag
    10561 atctgttgca aatagagaaa atataaaagt ttctcttgag atagtataaa ataggaggat
    10621 taggtataat gcctagtcct ttttatataa atgcgtagtt taagatttta tgatgatatg
    10681 accacataag attaaatttt ttaagaaggt gataacatga aagaaaaatt aactcatttt
    10741 aaggtaggtc aaatagtaaa tacacaaggt ttaaaaggtg aagtaagagt ttatccgctt
    10801 actgatgata tagagaggtt tgatgaatta aaagatttct atctgggaaa agatttaaat
    10861 aataaattgg ctgttgaaaa agtaagatac aaaggttcta tggttataat gaagataaaa
    10921 ggcatagaca gcatagagaa agcagaaaaa ctaaaaaata aatttatgta tgtagctaga
    10981 gaagatagca gagagcttga agaaggcgaa ttttttatag cagatatgat tggaatggaa
    11041 gttttaactg tagatggaaa atatgtaggg atactagaag atgttcttca atactcagca
    11101 aatgatgtct atgtagtaaa aggtgaggaa gataaagaat ttatgatacc agctataaaa
    11161 aaatttgtac ctacaataga tattgatgaa agaaaaatga ttatagaccc tattaaggga
    11221 atgatagatt aggtggtaat atgagatttc atataatgac actttttcca gaaatattta
    11281 attcgtacat ggatgaaagt ataatgaaga gagcagttga aaaaggtata attgaggttc
    11341 atatatataa tataagggat ttttctaata ataaacataa aaaagtagat gattatccat
    11401 ttggaggagg agcggggatg gtaatgactc ctcaacctat atatgacaca tataagcata
    11461 taataacaac tcataatata aataacccaa gtgtaattta tcttactcca aaaggtaagg
    11521 tatacaatca atctatggca aagcagatgt ctttaaaaga agatataata ctactatgtg
    11581 gtcattatga aggaatagat gagagaatca ttgatttaat agttactgat gaaatttcaa
    11641 ttggagatta tgtattaact gggggagagc tcccagcact tataatgata gattctatat
    11701 cgagattaat accaggtgta ttaaaccaag aagaatcatt tgaagaagaa tcatttaaag
    11761 acaacttatt ggaatatcct cattacacta gacctagaga ttttgaagga ttaaaagtac
    11821 cagaagtgct tttatcaggg aatcataaga agatagatga gtggagaaga gaagaatcta
    11881 taaggataac caaagaaaga cgatttgatt tatataaaaa atctaatgaa aaataaaaat
    11941 ataatgaaaa attaatatat atgtatacaa taaagtacct ataaatggaa ttaaaaacat
    12001 aaaagtgctt gtaataacta ttgaagtata gtataatata aaatgttgaa aatacgggcg
    12061 ttcctctgtt tacactggtc gggtagttaa gaacgtctat gatatctagg aggaagaaag
    12121 atgaatgaaa tgctaagagc aattgagcaa gaacaattaa aaaatgaagt tcctaatttt
    12181 ggacctgggg acacagttaa agtacacgtt agaattatag aaggaaaaag agaaagaata
    12241 caagtattcg aaggtgtagt attaaagaga caaggtggag gagcaagaga aactttcaca
    12301 gttagaaaaa tgtctttcaa tgttggtgta gaaagaactt tcccagttca ctctccaaaa
    12361 atagaaaaaa tagaagttac tagaaaaggt aaagtaagaa gagctaaact aaactactta
    12421 agaggtagag taggtaaagc tgctaagata aaagaagcta gaaataaata attaaaaact
    12481 ttacttataa atggggggct gatttatcag tcctctttct attttaatga aaattacagc
    12541 aaataattat actagaaata aatagaaata tttgttgaaa atatacatta tttatctatg
    12601 aatttatagc ttttaaaatg aaaaataaaa ttggtaaata ttgcattata ttttgtaaca
    12661 atattaaaaa taagagaaca gtattatgta aagtatgacg ttaaaaagat gacaatatta
    12721 tagtttgtta agatagattt tttaagtgga attaggatat aataatataa tattaataag
    12781 gagtgataca tgtgtcaaat gaatacgaag agtatcttat gaatgacaat ctacatataa
    12841 actggtatcc aggtcatatg aaaaaaacta aggagttagt aaaaaataac ttaaaattga
    12901 ttgatgtagt aattgaactt ttggatgcta gaatcccgtt tagtagtaaa aatccagaca
    12961 tagacaggct tgtaggagat aagccaagag ttgtcgtact taataagagt gatatggcag
    13021 atagggataa attgaatcaa tggattgaat actataagaa aattaatatc aaagctatac
    13081 cagtagatac aataaaaggc gttggtataa ataagataat cgaagaatgt aaaaatgtaa
    13141 ctagagaaaa aatgagttct ttaaaagaca aagggagaaa agaaagagct atacgaataa
    13201 tgatagttgg agttccaaac gttggtaaat cgtctcttat aaacaaatta acaggtagaa
    13261 aaagcactca aactggagat aagccaggag taacgaaagg gaaacaatgg gttagattaa
    13321 agggaaatct tgaactttta gatacacctg gaatactttg gcctaagttt gaagaccaag
    13381 aagttgcatt aaatttagca tttagtagag ctataaagga tgagatttta gatactgaaa
    13441 ctttggcttt aagacttata gagaaactta tgaaaataga acctgagaag ttgaaagcca
    13501 gatataagtt agattgtcta ggagaaactc caattgaaac tatggatatg attggccata
    13561 aaagagggtt tataacaggt aaaaaagaac tagattatac acgcatagcc acaactgtat
    13621 taaatgaatt tagagatgga aaaataggaa atataacatt ggaagttcca gaaaacgtta
    13681 aaagatagat tttaaaacaa aatcaaaaat ttaatgttaa aaatttaaaa aagcctttta
    13741 gcaaaggctt ttttattttt ttgaaatata gacaaatatc tccatataaa agtataaatt
    13801 aggttagaaa acctttaaaa atattaattt aagacaaata tacctaatat tttgaaaatt
    13861 aaaatgagtg tgattattta aaattttagc atattgactt gcttgtttcg tataatatat
    13921 aataagcaca aataaataca atatattaaa aatttaattt atttaatttt attaatttaa
    13981 taataagttc ttattgttta attcaactta cttatgcaag cgtaaacaat cgagacattt
    14041 tattaattta ataaggttaa aaagggggat tttataatgg gtaagaccaa ggatgttata
    14101 gtatttggat ttgcgttatt tgcaatgttc tttggagcag gtaacttaat atttccacct
    14161 tacttgggaa taattacagg tccagaatgg ttaattgcct tcttaggatt tacgtttgct
    14221 gatgctggat tggcacttct agcagtaatg gctacagcta aattcgatgg aaacgtagtt
    14281 gaaatgttta aaagatgtgg tatgaagtta ggaattctta taggttgtgc agatatttta
    14341 tgtataggac cattcttagc tataccaaga acaggtgcaa caacttatga aatgggtata
    14401 atgcctttgt ttggaacttc aattccagtg ttattattct gtatactttt ttttgcgatt
    14461 tcatatgtac taacaataag accttctaag gtcgtagata tagttggaca gtttttaact
    14521 ccagctcttc taatagcttt agcatttata attataaaag gtattatatc acctcttgga
    14581 gatatagtag ataaacctat gatacctaat gtatttgcag aaggtatagg tcaaggatat
    14641 cagacaatgg atgcttttgc agcaatagca cttgcatcag tgctaatagt atctttaaat
    14701 gataaaggat attctactat tagtgacaag ttgaaaatga ttggtaaggc gggtgttctt
    14761 gcttgtggag gattagctct agtttatgga ggattatgtt tccttggggc aactgtatca
    14821 acaatgtatg gaacagatgc agtacaatca caagtaatag taaatataac tgaaggattg
    14881 cttggaaatg ttgggaaagc gattttagca gtagtagtat cattagcatg tttaactaca
    14941 tctataggtc ttacatctgc cacaggtcaa tatttctcta gacttacaaa aggtaaattg
    15001 agttatgaaa aaatagtatt agctgtatct gtattcagtg cagtagtagc atcttttggt
    15061 gttggaacta taataaagat agcatctcct atactaagta tagtttatcc accatcaatt
    15121 gttttaataa ttttagcttt cttcaatgaa aaaattaaga atgataatgt atacaaagga
    15181 gcagtatata tgtcattgtt tgtaagtata ctaacagtta tatctagtta tggagtagct
    15241 gttccagtag taaattcttt acctcttaat tcattaggat ttaactgggt ggtgccagtt
    15301 ataatagctg gaataattgg taattttata ccatcaaaga gtcaatctaa tacacttgga
    15361 acaaactaag ttttgtttaa ttaaaatata atcaattgta aataaatgag ttaactaaaa
    15421 gtaaaaggag ttatttgtac aaataactcc t
//
LOCUS       DAMJZE010000095        15384 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJZE010000095 DAMJZE010000000
VERSION     DAMJZE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.096"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            107..1498
                     /locus_tag="PC541_002416"
     CDS             107..1498
                     /locus_tag="PC541_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2839875.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1751..3544
                     /locus_tag="PC541_002417"
     CDS             1751..3544
                     /locus_tag="PC541_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2839876.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3625..5007)
                     /locus_tag="PC541_002418"
     CDS             complement(3625..5007)
                     /locus_tag="PC541_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2839877.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5265..5612
                     /locus_tag="PC541_002419"
     CDS             5265..5612
                     /locus_tag="PC541_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2839878.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5630..6664
                     /locus_tag="PC541_002420"
     CDS             5630..6664
                     /locus_tag="PC541_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839879.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6713..7762)
                     /locus_tag="PC541_002421"
     CDS             complement(6713..7762)
                     /locus_tag="PC541_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2839880.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8037..9572
                     /locus_tag="PC541_002422"
     CDS             8037..9572
                     /locus_tag="PC541_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839881.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9757..10443)
                     /locus_tag="PC541_002423"
     CDS             complement(9757..10443)
                     /locus_tag="PC541_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2839882.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10568..11950
                     /locus_tag="PC541_002424"
     CDS             10568..11950
                     /locus_tag="PC541_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2839883.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12352..13620
                     /gene="eam"
                     /locus_tag="PC541_002425"
     CDS             12352..13620
                     /gene="eam"
                     /locus_tag="PC541_002425"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2839884.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13713..14069)
                     /locus_tag="PC541_002426"
     CDS             complement(13713..14069)
                     /locus_tag="PC541_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839885.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14220..15314)
                     /locus_tag="PC541_002427"
     CDS             complement(14220..15314)
                     /locus_tag="PC541_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2839886.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5943 a         1912 c         2485 g         5044 t
ORIGIN      
        1 atctattgag taaacataga aaagatgata ttataagtat gtgctaaaat ggttaaagca
       61 atattaatat taaatttaac tataaaacaa aagggggcgt ttttttatgt caagtacaga
      121 aagcgtatct aaaaaaagtg gtcatccgaa gagtttttgg cttatttgca tgacaatttt
      181 gtgggaaaga tttagttatt atggtttaac agctttattg gtattatttt ttacagcaag
      241 tgtagctcaa ggtggtttag gtctttctac tcaagaagca acatctatgt ttggcttatt
      301 tacaggtctt atatatctta caccattagt cggtggctgg ttagcagata aagttttagg
      361 tcaacaaaaa tgtatttcta taggtgcact tttaatagca ataggagatt tcgtactatt
      421 tgcttcatct acatctattg caatggtttg ggtaggtcta tcaataatta ttttaggaaa
      481 tggttttttt aaatcaagtt gctcaaatat agttggggat atatatccta aaactgatgt
      541 gtcaagaaaa gatgcagcat acagtttatt ctatatggca gtaaatgtag gagcatttat
      601 agctccaata atatgtggat tgctttctga taatgtattt gcagtaagag gagcaagtgg
      661 agatatagta tcttatggat ataagatggc atttttaatt tgtggtatag gcatgatgtt
      721 aggtatgatt atatttacac ttcttgcacc aaaattatta aatgaagtcg gaaaatatcc
      781 tgtgactaaa ggtaataaag gtgaaaaaat tgagtatgga ccacttacta agttggagaa
      841 aaataggata atagcaatgg ttattttatt tttatttgtg gtcttattct ggacagcata
      901 ctatcaaaca caaagttctt ttatgattta tgtaagggat aatgtaaata gaactctatt
      961 tggatttgaa atgccagtag catggcttac atcactaaat gctatattat gtgtggcttt
     1021 atgcccagta ttagctaccc tatgggttaa attatctcat accaaaagag gtgatttctc
     1081 tattccagtt aaaatgggat taggaatgat aataatgggt gttgggttta tagttatggt
     1141 atttgctaca ttagctaatg gtccagatgg aatggtaaag gctagtatat tgtttatagt
     1201 attgacttat atatttacaa cagtaggtga attattctta tctcctatag gacttgcaat
     1261 gttcaataaa ttagctccag ctaagtatgc tacgttgtct atgggagcct ggtatctaac
     1321 aagttttttt gcaagtttat tatcaggaaa gattgctggt tttacagcca cacttggttt
     1381 tttacagata tttggaggaa tagcaacagt tttaataata tttggtttaa tattattggg
     1441 aataagaaat gtattaacaa gattaatggc tatggattta ctaacaaaag aaaaataatt
     1501 catgacgaat tattgtaaat aaatgaatta ctttaaataa aggaagttac atttaaatat
     1561 aaaattaaat gtaatttcct ctttgattat gaaaaaaagc tttacacaaa tagatattat
     1621 aattaaaatt ataattgtga taattagagt gtataagatt tatataaatt atacaaaaaa
     1681 tatttatgta ataaacttta catacttata tatttagtta ttcaaactat tatttcgagg
     1741 aggtcttgtt gtgaatataa aagatagatt aagtggatta agaaaattta tggaagaaaa
     1801 aaatatagat gcctatatga ttccatcttc tgataatcac caaagtgaat atgtaggaga
     1861 ttacttcaaa tcaagagaat ttatatcagg gtttaatggt tcagcaggga cggttattgt
     1921 gactaaagac gaagctgggt tatggacaga tggaagatat tttatacagg cagaatctca
     1981 attagaagga agtacaataa aattattcaa aatgggacaa gaaggttgcc caactacaga
     2041 tgaatattta tataaaaata ttcctgaagg aggaacttta ggatttgatg gtagagttat
     2101 atctgctaga gaaggagcta cattagctga aaaattatct aaaaaaggta taaagataga
     2161 atatcagtat gatttaatag atagtatatg gccagataga cctgctttat cagatagtaa
     2221 agcattttta ttagatgtta aatattgtgg agaaagtttt agctcaaaat tagctagatt
     2281 aagagaaaaa atgagtgaaa aaggtacttc tactcatgta ataactactc ttgatgatat
     2341 agcatggtta ttcaatataa gaggtggaga tgttaaatat aatccagttg tactttctta
     2401 tgcagtaata actttaaaag aagtttatct atttgttgat gaaagtaagt taaatgaaga
     2461 aatactaaat gaactagcta aggaaaatgt tcaaataaag ccatataatg atgtgtatga
     2521 atttgtaaaa aatatagaca aaactgaaaa agttttatta gatggaacta agcttagtta
     2581 tacaatttac aataatatac cttgtgaagt tgagaaagtt gatgagttca atccagtaat
     2641 gttttttaaa gctcaaaaaa atgaggtaga attagaaaat ataagaaatt cacatgtaaa
     2701 agatggtgta gctttcacta aatttatgta ctggttaaag aaaaatgtag gtaagatgga
     2761 aatcactgaa ataagtgcta ctcaaaaatt agaggactta agaagagagc aagaaggttt
     2821 ctttgagcca agtttcaata caattgctgc atataaagag catgctgcaa tgatgcacta
     2881 tagtgcaact ccagagtcaa attataaatt agaggcagaa ggattatttt tagtagattc
     2941 aggtggacaa tattatgatg gaactactga tataactagg actacagttt taggacctat
     3001 aagtgatgaa ttaaaactac actttacttc tgtagcaaga ggaatgataa atctttctaa
     3061 ggctaagttc ttgcatggtt gtagaggata taacttagac atactatcta gaagttgcat
     3121 gtggaatatg ggaattgatt atcaatgtgg aactggacat ggtataggat ttgtgctaaa
     3181 tgttcacgaa gctccaaatg gatttagatg gagagtagta cctgaaagat ttgatagtgc
     3241 agttcttgaa gaaggaatgg taactactaa tgaaccaggt atatatatag aaggttctca
     3301 tggtataaga acagagaatg aaattgtagt tagaaaagct gaaaagaatt tctatggtca
     3361 atttatggaa tttgaagttg ttacgttagc tcctattgac ttagatggta tagcaccaga
     3421 attaatgaat aaggatgaaa aagattattt aaattggtat cataagttag tttatgataa
     3481 aatatcacct ttcttaactg atgaagaaag agaatggtta aaagtttaca ctagagctat
     3541 atagtttgta ctagatacaa taaaaatcga ggtattataa tgcctcgatt ttttattgta
     3601 tattttatta actttaaaaa atttttaatt tgataattct actaattttt tattcattat
     3661 aagtagtata attccaaata tcaataaaaa tgatggtatc actatgaaga cttgtaacat
     3721 accaagttgg tctataatag cttgcgtata gccagataat ttatttgctg caaatttgcc
     3781 taaaaaccaa acacccatta ggaatgaaat atacttttca ggcgaaagtt tatttaccat
     3841 accatacccg actggagaaa agcaaatttc tcctatactt tggaatgtta aaaatccaac
     3901 tacccaaaat acacttgctt tgtgaattga attagggtct gttcctgttt gtataactgc
     3961 tactatcata aacaaaaatg ctatggctaa tagtataaaa cctaagctca ttttttttgg
     4021 cacacttaaa tcgccttttt tagtttgaga taattttacc catactaaag cagagatagg
     4081 tccaagaata acacaaagga gaccattgta tgaatctatc catgaagtag gaatttgaaa
     4141 attgccaaca gtaagattta tgtgttcctt tgtatagagt gcaatagaca tagaggcttg
     4201 gttatatgct atccaaaaaa atactgtgaa aaaagctaac accaatatta ctattattct
     4261 ttttttctca atcaaagtta aaggtcttga agcgagcatt ttcttatatt ctagtgattt
     4321 tcttatagac tctctcccgt taatagggtc attattcaaa ggtttataaa actttctgtt
     4381 tccaaatata aagaagaata aacctattag aaccgtaatt gatgaaaata aaaatacata
     4441 tctatatcca tacatactta tagataatcc gcttttcata gcaaaccatt cattagcaac
     4501 taaaccgcat attaaagggc caaataaagt acctagatta gtagccatat acgtaataga
     4561 gaaagctcca tcttttcttg ggtcatcatt ggtatataag tttccaacca tagtatgtaa
     4621 gtttccttta taaaaaccta ttcctaatcc agataaaaat atcatgatgt ttatatccat
     4681 cttactattt gctatgtatc ctagaaaata accaattgaa accattagca ttccaagtat
     4741 aatacagttt ttgggaccta aaaatctatc actgacccat ccaccaataa ctggagagaa
     4801 ataagtccat gctaaaaaag atgcaagcat tgatgcggat tctgctggtg ttaaccctaa
     4861 accacctttg gagacttgtt cagctaaaaa caataataat accaatttca taccatagta
     4921 tgcaagtcct acaaaggtaa atattgaaca gcagaaatat aaatctctag gttgtttaag
     4981 tttttcttgc gtttttaaaa ctgacatatg tcgagaacct ccagataaat agtttaacaa
     5041 aattttacca tactatatga gtatatgttt ttttcatata gacgtcaact tgtaagataa
     5101 tttaattaag tcgaaatata tgtaaaaata gtatcatttt tgtgtattca gtaatatttt
     5161 tttatgataa tatatgttat tattatatta aaatacaaac atatttatgg aaaggatatg
     5221 ccaatttagt agttagattg attgtttggc aggtgggatg agctatgata agaaagaaat
     5281 ctgaattaca agtagaagta aaagaaaatt taaatggtgg tataggaaag gttaagcttg
     5341 aaaacataat acaaaatgaa gagcttaaag gaaaaggaag actatttaaa agagtagctt
     5401 taccagtagg aagttcaatt ggtgtgcatg accatacaac agattttgaa gtatattata
     5461 tactaaaggg aaaaggtaaa gtatttgata atggggaatt tgtagaagta aatgagggtg
     5521 atgtagtgta tacagctgat ggcgaaaaac attctataga aaatatagga gaagaagatc
     5581 ttgagtttgt agcactagtt ttatatatgt aaaatatgga ggaggagtca tgacaggcaa
     5641 gatagaagaa attaaggaca agataaatag attttgcaac tccaatttaa atcaagaata
     5701 caaagatata agctttaaaa ttttgcaaaa ccttttagat aataataaag agataattca
     5761 ttcttctaga gctgatatat ggtcttcagc tatactaaat atagttttag aacaaaatct
     5821 tttatacaat aagaagcatc cactacatat aacaaaaaaa gagttttcaa aacaaatagg
     5881 tgtttcttta aatactataa ataataaatc gtctttaata aaagatgttt gtagtattga
     5941 ctttttaaat caagatacta tagctatttc taatgtagaa ttttgggtaa aagagactaa
     6001 ttccaaatca aagtctgtag atttagattt agaaaagaag aaaatactat ataaaagata
     6061 tattaagcta tcacaagatg cgaatagtga tattgaatca attagatata tggaggaagc
     6121 tgtaaatata ggtaaaagta tgattactaa agaagataaa gaacttggat tttggaaagg
     6181 tttatctacc agagcctata tgatagcact tgaatccctt gctagaaagt tagaaagtat
     6241 taataatcta aaggagtcca aaaaagtatt agaatattta atagaaatta atcctagtga
     6301 tgagcaggga ataagataca aattattaaa tgtattaatt agattaaatg atagaacttc
     6361 aataaataaa ttatttgaac tttataaaga agaaaaatct gctacatgga tgtacagtaa
     6421 agctctttat tactttaaaa ataaaagtat gtttttggca aatgattcta ttaaggttgc
     6481 tagaagtaaa aataagtacg ttggattata tttgttagat tggcgaaatg cttttggtag
     6541 agaatttgtt acagaggaag aaaaggcaga agctgtctat tactatgatg aaaacatagt
     6601 gatttggaat gaagttaaag gttctatgga ttggctatta aaaaaaatgt tggaattttc
     6661 ataaaaaaaa ctactcctta cttttatata agtcaaagga gtagttttta atttatacat
     6721 ctagttttaa atcttcaggt tttattagac ctttttttct cataaattct gaaatcaaca
     6781 aagttacaat agcaggaagt ataaaattaa gtaaaagtat accaacccac atagatatac
     6841 ctccttttcc agcactttgc atagcagtta ctgttccaaa ttgtccaacc aatccacaag
     6901 tacccatacc agaaccaata ggaatattct ccattttgaa tattgttgta gataatggtc
     6961 caagtaatat tgaagctaca gtaggtggta tccaaatttt ccaatttttt attatattag
     7021 gtacttgcaa catagatgtc ccaagacctt gtgccacaag gccaccaact ccattttctc
     7081 taaagctcat aacagcaaag cctaccattt ggcaacaaca acctacagtt gctgcaccac
     7141 cagcaagacc acctaaactt aacatcatac aaatagctgc actacttata ggtaatgtaa
     7201 gagctattcc aacaagtgca gagactaaag ctcccattat tataggttgc atagttgtag
     7261 cttgcattat tatatttcca aatgaagtca taaaagcaga aacagctgga ccaataagtt
     7321 cagctacaag tactccaatt agtattgtta cagcaggtgt aaccaatata tctatttttg
     7381 tttcttttga aactaatttt ccaacctcta cagcaaaaag tgtagcgaca aatgctccaa
     7441 caggtcctcc taattcatta ccacaagctc caacaagtgc acaagaaaat agtacaagtg
     7501 gaggtgcttg aagtccatac gcaatagcca ctgctatagc tggaccagta acctgtttag
     7561 caagagggct tataacttca gtaaaaagag aaatattgaa attatctcct aatgtattga
     7621 aaattgttcc aattaaaagg gacgaaaaaa gaccgagtgc catataactt agtgcgtcaa
     7681 taagatacct ttgaacagaa atttcaatat tttttctttt agcaaatgat ttgaaatttc
     7741 tttttttata attgatttcc aatttaaaat cctcctcata taatataagt ataatttctt
     7801 tttaattaat tatataactt ttcagctgac aagacaaatg ttatttcact aaaattagac
     7861 tattgtaata gtaagaaatt aacattaatt ttagttttaa attttaaatt tatggtttat
     7921 aattagtata gggatattct gaaatataat aattttccag tttatcccaa tatttaactt
     7981 aggtagattg cttacaaaac aaatgattat atattaggct aaaaggaagt ggatagatga
     8041 tatttgtaaa aaataagaaa tacagaaaaa ttgccataat tatatctatt cctatattgc
     8101 ttgttgcgca gtactacctt tttaaatttg gaatacttag accaatggta aagggagtag
     8161 aaattgaaat tgtagatggg gaatatgtta aagatataga taaatatgta gtaaaattaa
     8221 atgaaacagt tactttgtct acaggtgaat atattaaggt tccttcgtat gctaaaaatc
     8281 caaatatttg gtttaatgta ttggataact cagggacttt aaaaatagat ggaaataaaa
     8341 tgacagcatt aaaggttggg tattcttctg tgggtatcat gaaaaattca agagtattaa
     8401 aaaaggctac tataaaagta gtagacccag aggtagaatc actagatatg gagattaatg
     8461 gggatttaaa acatgttggt gatagtgcta ctatagaaag tacaatagaa gttgattatg
     8521 ataagtttaa aaaatcatat aagccagttt ataagagtag caatgaaaat attttaaaaa
     8581 tcaaaggaaa taaagtaagt gcagttggag ttggtaaagc tacaatatat gctaagtgtg
     8641 gaaataaaga aattgagaga acatttagga ttcaagctag agttgctgaa ataaatatgg
     8701 atgatataaa aatagaagtt gaccagaatg aaaaattgaa accagatata ataacttctc
     8761 cgcctggttt agaaccacca aaaatatatt atgagttagt agattcaaaa ttacctgtag
     8821 aaagagcaat aagtttaagt agtaatggaa atgttgtagg gcttagagaa ggtaaagata
     8881 gggtgagaat aacttgtgga gataaatcta aagtaataac agtaacagta gtcaaagaaa
     8941 ctataacgaa tgactatata caaaacttgg ttggttcaca taaaatagta aacaataaag
     9001 ttattattaa acttgaatgg gattatatga aagatgtctt tgattatgat atatatctta
     9061 aagataagtt gtcaggagaa tcatttaaga aaattaaatc tataactata aatgaaagtg
     9121 atttaggtaa gtctaacaag gtatatgcaa ctatagaagt agatatgaag ggtaatcaaa
     9181 atatagattt tgatatttat gtcataggga aaaaggattc agatacaact aagccaagta
     9241 atgtaataaa tattaccaat ggaaatggaa attcaaataa tgataataat attaaagata
     9301 tgagggtaga aaatatatca gctaatgttg atagagataa taatgtcatt agagttaact
     9361 ggagtagtat atctcataag gactgtactt acagtatata tgtaaaaaat aatactaatg
     9421 gagatggaga atttactcta tatcaaagtg atatacaggg aaatgagcat actataagta
     9481 ttcctgaagg agatttaaat ctagatattt atgttgttgc aaattataat ggaaatagct
     9541 ctcaaaatag tgatgttata aatgtaagat agatttgtat acaaattgaa agtgttgagt
     9601 ttatttaaat attaaattaa gaaaaaggat agtatagggt aattatacta tccttttttg
     9661 ttacaatata taggtttaaa atctctagta atataaatta taaaaagtct tatttacaca
     9721 aatctataga tatttttttt attaatcaat attgtcttaa attgaactta gtgcggcttc
     9781 tattgcatgt attactgtcg tatcaaatag tggaatagaa gtgtcatttt gtttaattaa
     9841 aagacctatt tctgtacatc ctaatataac agcctctgca ccttgtttgc caagcttatc
     9901 tataatagat aagaaagctt tttttgaaga ttcagatata attcctaagc aaagttcatt
     9961 aaatattgtg tcatttacta ttatacggtc ttcttcattt ggaattaaga cttcaattcc
    10021 attattaata attacatttt tataaaaatc ttgttccatt gtatatttag tacctaataa
    10081 ggccactttt tttatttctt tttcccttaa taccgtagca gttacatctg ctatgtgaag
    10141 taatggaata tgtatacttt cttgtatttt atctgatacc ttatgcatag tattagtaca
    10201 gataacaata aaatctgccc ctgcctcttg taatttaatt gcagcatcag ctaaaatttt
    10261 ggcgcttttt tcccaatttc cactagattg gcattcttca atttcttgaa aatctacact
    10321 gtaaagtata catttagaag agtgtagtcc acctaatctt tcttttatta ctgtattaat
    10381 aacttgataa tatgtaatgg tactttccca actcatacca ccaattaagc ctatagtctt
    10441 catattatac ctctcttatt tatttatttt gaaatagcta tttacaaata caagtatagc
    10501 atctaaaata gaagcataca atgataaaat tgtatggagt acaattataa ttaagaggag
    10561 taattatatg ccaataaatt cttttgaaaa ctatcctatg acatggaaac caaaattaga
    10621 taaccgaaaa ccaccaatat ataaaacttt agcaatgtta ttagaagaag atattaaaag
    10681 aggaaattta aatcctggag ataaactgcc tccacaacgg gaattagcag attttctaga
    10741 tttaaactta agtacaataa cacgagcatt taaactttgc gaagaaaagg gactaatatg
    10801 tgcaaaagtg ggaaagggaa cattcatttc ttctgatgta aatgtaagta atacattatt
    10861 atatcaaacg gaatcaaaag acataattga attagggaca gttcatcctc cgtatgagca
    10921 aaatacatat attattgact ttattaaaaa tgtactaaaa caaccagaaa tggataggtt
    10981 tttacaatac atgtctccat ctggcacata tatgcaaaaa aagtcaattg caaaatggat
    11041 agaaagaaat aatgtataca ccagtgaaga aaatatatta ttatctacag gagggcaaaa
    11101 tgctatatgt gcaactttac taggattatt taaagctggt gatagaattg ctacagattc
    11161 tttaagtttt tctggaatta agtcaatagc taaaatgata ggtattcaat tagtcccaat
    11221 tcctcaagaa aataatgaaa tatcgattga atatcttgaa agttattgta aaaatgaaaa
    11281 tataaaaggc atatatctta taccagatta tcataatcca acgacacata ctatgtctga
    11341 tttatctcgt aaaaagatag ccaagatagc aaagcaatat aatataataa ttattgaaga
    11401 tgctataaat agcattttta gagatggtat acaaactccc atattttctt tagctagtga
    11461 taatactata tatattttta gtacatctaa atttttatgt gctggtctta gagtagcatt
    11521 tgtagttgct ccaaaacgat atatagaaaa tttagaaaat gcactatata atatgaatct
    11581 aatggtttct ccatttacag cagaaatagt acaccgttta ttatattctc ctattataga
    11641 taaaattata gaagagaaaa gagatgcaat tatagaaaga aatgaagttg ctgataaagt
    11701 tttgtcagat tataatttga ttggtgataa gaattgcagt tttcgttggt tacttcttcc
    11761 tgatgaattg gatggaaaat catttgaaat ttctgcaaaa aatctaggag ttcaagtata
    11821 ttgtgctgaa cgattttcag taggaaactc tacagttcca aaagcagttc gtatttgtgt
    11881 aacagctcca aaagatgtag aagaattaga aaaagggctt aatataataa agtcacttct
    11941 taagagttaa atttatttta ctattattta attaaataaa taaaaaggcg gagaaattta
    12001 ttataataaa cctccgcttt tattatttaa tgaaatactt tactttttat taaaaaattt
    12061 aattttaata atcttgaaaa tataatcgtt ttatggtaaa ataattctta aaaatagaaa
    12121 aaattaatca tttgttaaaa aaaatgcata atttattgta atttattgac gaaggttgaa
    12181 aaatattcat aaatgtgcga aataacagaa aaaatgcaaa aattaattca atttcatttg
    12241 ttataaaatc tatatggcaa aattaaatct gcctatcaga atgatataaa cacaccagaa
    12301 aggaggacgc tatcaaataa tacaagtagt taactttgat agcaaaaaat tatgaatgaa
    12361 caaactagaa tatccttaga gagagctgct gaattaaaat caaaaattga tgattatatt
    12421 caggctagaa aaacgattaa cagaggtctt gaaaaagaag aagagataaa taaacgaaaa
    12481 cagaaaatat taagtatctt aaatggaact gaagaggatt ggaataacta caaatggcaa
    12541 ttatcaaata gaataacaga tgtagatact ttatcaaaaa ttataactct aactaaaaaa
    12601 gaaaaagaat atataaaaga ggttggtact caatttagat gggcaatatc tccatattat
    12661 ttgagtctta tagacccaga agatacatgt gacccaataa aattactgtc tataccaaca
    12721 catatagagt tggaagatga acaagaagat ttggacccaa tgggagaaga gtatacaaac
    12781 ccagcaggat gtataactag aagatacccg gatagattaa taataaatgt aacaaatgag
    12841 tgtgctatgt attgtagaca ctgtcagaga agaagaaata ttggacaaca agattctcat
    12901 aagtcaaaag ctattatcca agaatctata gactatatca gagaaaatga agaaataaga
    12961 gatgtactag taactggtgg agatgctctt acattaaaag atgattattt agagtggatt
    13021 cttagccaac ttaaagagat accacatgtt gattatgtta gattaggtac taggactctt
    13081 gttacaatgc cacaaagaat cacagatgaa ttttgcaata tgctaaaaaa atatcaccct
    13141 gtatatataa atactcattt taatcatcca atggaaataa ctaaggaatc taaagaagct
    13201 tgtgaaaagt tagcaaatgc aggagttcca ttaggaaatc aggcagtatt attaaatgga
    13261 ataaataatg ataaatttgt aatgagatgt ttaaatcaag aattactgaa aataagagta
    13321 aaaccttatt atatattcca aagtaaacat gtaaagggaa caaaacattt caatacatca
    13381 gtagatgatg gtcttgaaat catggagtat ttaagaggat atacatcagg aatggctata
    13441 ccaacatata tagtaaatgc tccaaaagga ggcggaaaga ctcctttgct tcctcaatac
    13501 cttgtatcaa aaggaacaga ttacgttatg cttagaacat gggaaggaaa agttataaaa
    13561 atggaagatg aacctgctgt agatataaag aaacttataa aagaacaagc gcaggattaa
    13621 aaaagtattt aaagtttata aaaagaaaca actttaaaat aaaaagaaaa tggatgtcaa
    13681 atttatatca atttgcatcc attttttatt agttatttat tattttgttg ttgattaact
    13741 aattcaagat atccacttgg gtggaagtaa tatatctcag aagtattttc atttacaaag
    13801 taaccaatat caccatctgc atctggtaaa aatacataac cattaagatt tttagctttt
    13861 aatgcttcaa aatcactagc atttccttga tatatgtaat caacttcatt gttgtatttt
    13921 actaaaagtt ctttagcttg ttcttgagtc aatttagtat tttggcttac aggaacattc
    13981 ttttgagaat ctgcataaga agattcattt actggtgcag ttaataacaa acaagataaa
    14041 actaataaag atataagtgc ttttttcata ctaaaaccct ctttcatgtt taattatata
    14101 agattcatta cttaataata ttgtatgttt cctataatta aatattacca ttaaagagaa
    14161 taatattaca gttacacagc tgtaactttt gattacaaaa tcgtaattaa attgtatttt
    14221 tattccattt gtattttatc atgtctacga tttacaaact cacagaagta tatctttata
    14281 acgcccatca caggagcaga tagaatcata ccaggtatac caaagaatcc tccaccaaca
    14341 gtaactgcta ggattgtaaa gaatgggcta agacctagtt gtccacctac gatttttggc
    14401 tctataaatg taacttcaac ttgttgaatt attattaagt aaattaaaga agttaaagca
    14461 attgttggat tggagaataa attaattatt acaactggtg ccatacctat tactggacca
    14521 aaatatggaa ccatattcat gacacccata agtgtaccaa ataatagtgc atattcagat
    14581 tttaaaaagt aaagaccaat tccagaaagt actccaacta taaaagaatc taaaatctta
    14641 cctgtaaagt atttaccaat atttaaattt aagctttgac atagttctat tataaagtct
    14701 ccatattttt tacctaagga aatataaact actttttttg taaacagtaa gaatttttct
    14761 ttttctaata atatatagaa acaaattata aaacctaaaa gaaattgtac tataaatttt
    14821 ccaactgaaa atgtagtgct aaatatccca ccaagtgaac caataagcaa gttactaaat
    14881 ttaggcatta cactcataat tttgtcacca tactctttta atgtattagg gtcaactgat
    14941 tgtaaactct tgcctaagtc gaataaaaac tgctctgttt tatctatgta tgtaggaatt
    15001 tgagcaacta aatcagctaa actatttact atggatggaa ctgtaaataa tataaacgaa
    15061 attagtataa ctatgagtac accataagta agaagtaacg aatatattct cttaaaattt
    15121 aatttacttt ctaagaactt tactagtggg ttgaatatat atgctaatac aaatgctatt
    15181 ataaatggtg ttagtagtga cattaataaa cttatcactc caaaaaaata tttgtaatta
    15241 tcaataactt taatcaagat atatgaaaca actacagtga gtacaatatt tagataaatt
    15301 tttttatctt gcaaaataac acaacctttc ttatatttat taaatttaga tatacttaaa
    15361 tctaagttaa agttaatatt tata
//
LOCUS       DAMJZE010000096        15143 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJZE010000096 DAMJZE010000000
VERSION     DAMJZE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.097"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(108..323)
                     /locus_tag="PC541_002428"
     CDS             complement(108..323)
                     /locus_tag="PC541_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839887.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(506..850)
                     /locus_tag="PC541_002429"
     CDS             complement(506..850)
                     /locus_tag="PC541_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839888.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            977..1360
                     /locus_tag="PC541_002430"
     CDS             977..1360
                     /locus_tag="PC541_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839889.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            complement(2570..2821)
                     /locus_tag="PC541_002431"
     CDS             complement(2570..2821)
                     /locus_tag="PC541_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839890.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(3067..3222)
                     /locus_tag="PC541_002432"
     CDS             complement(3067..3222)
                     /locus_tag="PC541_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2839891.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            3933..4157
                     /locus_tag="PC541_002433"
     CDS             3933..4157
                     /locus_tag="PC541_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839892.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     repeat_region   4444..4736
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4509..4537
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(5390..5542)
                     /locus_tag="PC541_002434"
     CDS             complement(5390..5542)
                     /locus_tag="PC541_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839893.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     gene            complement(5692..7119)
                     /locus_tag="PC541_002435"
     CDS             complement(5692..7119)
                     /locus_tag="PC541_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HDF2839894.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISVNALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            7310..8131
                     /locus_tag="PC541_002436"
     CDS             7310..8131
                     /locus_tag="PC541_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2839895.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            8256..9773
                     /locus_tag="PC541_002437"
     CDS             8256..9773
                     /locus_tag="PC541_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2839896.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKAYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            9796..10080
                     /locus_tag="PC541_002438"
     CDS             9796..10080
                     /locus_tag="PC541_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2839897.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(10177..11847)
                     /locus_tag="PC541_002439"
     CDS             complement(10177..11847)
                     /locus_tag="PC541_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2839898.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(11919..13166)
                     /locus_tag="PC541_002440"
     CDS             complement(11919..13166)
                     /locus_tag="PC541_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2839899.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(13153..13827)
                     /locus_tag="PC541_002441"
     CDS             complement(13153..13827)
                     /locus_tag="PC541_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2839900.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(13999..15078)
                     /gene="mltG"
                     /locus_tag="PC541_002442"
     CDS             complement(13999..15078)
                     /gene="mltG"
                     /locus_tag="PC541_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2839901.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         5238 a         2305 c         1722 g         5878 t
ORIGIN      
        1 tgttactata acataggaat tatctttaaa aacacaaatt aatttacatt ctctaatttt
       61 aaagtatatg tatatctttg attttaataa aattcactta aaatatttta aggttgaaca
      121 atgaaattga atgcccaaga acttcctcct cttactaatg ttgcaattaa ttcaaaccca
      181 caattgtatt gaactgaatc tctacctact ggtttccaac tagaaggtgc attatatgag
      241 aaagaatttg atgttggctc tttcttttgt tgctgcttaa ttcctagctc attagaaata
      301 aatccagttt gatttatatg cacccttcta tttgttacac gaatctggcc gtctgcaata
      361 gaccagtttc cagaaacttt actaacggaa atatattgaa atgctccatc tccttttgat
      421 attgtatatg taactgtacc agatgctttt acaccaccag ttttatccca tccatcttct
      481 gatatacttg cttttctaag aggctttatt gtattagtat caaaaaaact gtcttagaat
      541 atatctttga tgttaaattt ttagattgtt taacttctaa taattgagtt gttgatatag
      601 aatcaacgta tatggactct cctgttcttg catttttgat tatacctttt tcattggaac
      661 ttttaacatc agtaattcca tttttagcca tatcgtataa tttgtccatg tctgttactt
      721 gtttctctga atacattact ttcatagatg aatcttctac cttactagca cttgcatttg
      781 ttggtaatag tattgtcatt atgagtaaag ataaaatagt tgttacagat ttttttaacc
      841 tataattcat attaatcctt actttttatt tgttataaca gacattctat tgtaatagaa
      901 tataaatcca caatattttt aaaattagta ttgaataact attctcatgt tataattatt
      961 caaaggaggg attgatatga ggaaaaaatt ttttataggt attcttatag ttttaataat
     1021 ctctgtgata gctatactta atataaaaaa tgataaaaat gtctttattg gagaatcaga
     1081 aaattggaaa gctaaatttg taactactga aggacactca aaattagaaa taaaatataa
     1141 aaatagtaaa gatgtagttg aacaaaaatt taattattcc tatcaattta tagatggtga
     1201 agatgcagtt gtagttgtaa aaaatgaaat tttagaagca ggtcaagaaa aagcaataaa
     1261 aatacaaaga ccaggtcttt attcaaatag agaatctagt tacaagctaa ttatatcttg
     1321 gaatggaaat gaggaaacta taaacttgaa aaagaaataa ccatattatc aatttttaaa
     1381 aaattattat tcaaattctt ataaaaagag tgtttagaaa gatgaaacac tcttttttgt
     1441 ataagttttt gaaaactata aatgaaatat atcaaaatcg ttcgaaaaat tactgaaata
     1501 aactcttaaa tatattataa attaaggtat attatacatg aatttattgg gagtttatga
     1561 gttttaaaat tggaaaccaa acaataaggg cgaaaaatac ttcaaggaaa atatataaag
     1621 ttctataaat tatcaaaaat atttcgcaaa aattgataat tattacaaat aaattgataa
     1681 tattagaaat atcgaaattg aacaaactag gattaaagct caattagaaa aatccactaa
     1741 tcctgaaaat actttgttat tctcatttat tactgttgtt ttttcattat ttgctaattt
     1801 atttttcagt tcattaaatg ttaaatataa ctctataaac ttaattttac agttaatatg
     1861 tattatagtt cttatattta tatatattaa tacattttta tatcgcctct tcaatttgag
     1921 ccccatcatt atataaaatt aacctacttg atatcttaat ttaatatatg tacactattc
     1981 atatcttaat agtcaatatt attcattttt aatagctctt tttagatgtc agaattatta
     2041 tattttgaaa tttattaaat ttttgcatta aaaaaactag aaattaattt aagtcttgaa
     2101 ttacttataa ctttctacgt atctaccttt atattcttac tctgatttta tatctaatat
     2161 taattcttca tcaaagctat taaatatacc tctaattgag ttaattattt cacttacttc
     2221 acctttaatt tttatattaa cttcgaccat ttcaaatctc atctttttat cttataattt
     2281 ctataagttt ttttatttat tgtgttgcta ctttgaccag cactttttta tttaatagtc
     2341 taaatgatat tttttaccag ttttaatatc cttaaatact atatttacta aaaacttata
     2401 atttctttta atacatagtt aatatattct tattactaat aacaatactc aacaatttta
     2461 tcgccttttt tcaaatatga tttaactttt ttattcctaa ttatttaaaa atagttgtcc
     2521 aattttcttc tatgagaaag tgctaagtat tagcaacttc tctattctct taactttcta
     2581 ctgtctcaac tttcttcata gctatattaa tagctacttc tttagctact tcattgaact
     2641 cttcccatct tctcctattt tcttcatcag atatacgtgg atatactata taaacttctg
     2701 tgttttgatt agctataatt ttttcatcat attctatatt tttttcttta tcatatttgt
     2761 atggtttaaa ttgttgtagt attttcataa catcacccct tgttataaaa tatgaactca
     2821 taaacttgtc ttatacttat ttaattgtta caatttttac ttaaaatcac tttatattct
     2881 atattttaaa gctaaatcct ttacaaaagt aacatattct tctataagtt tcttatcctc
     2941 ttacatagta tctagtttgt ttaacttatc tcttttaaac ttacatacgc cttcttctgc
     3001 cattcgtctt ttattaattg ttaaccttgt ttcaagagac acaccaaaac tcttgttaag
     3061 aagctcttag ctctcattag caaaatattt tacataactt aaatcacctt gacttctagc
     3121 tattttaact gttaatagac ttgtttcttt tttccaatgt gttatattta atataacaac
     3181 atcttttatt attttaactt ctatttttac atctaatgcc atattattgc tttattaatt
     3241 tgtatcctta aatcttaaat tttctattaa tacatcttct atacagctta gtttctctcg
     3301 cttaatcttg aaatatattt tctctaaatt gtcaaattgt tcccaagttt tatcaatatc
     3361 taatattttg cagtgtctat ttgcacatct ttcgatccaa aggtataatt gactagaatg
     3421 ttttgaaatc aactgactat taatagatta gttcttaaat tctttcaaat actcgaattg
     3481 taataaaaca taatttacat tctctttatt tctattgaag ttagtttata tattgtttac
     3541 atcattttca tatatatctg ctagttgcta tgcatttaaa attctcttat tatttcttta
     3601 tattacttgt aaattattca tcctcatcat cctcttaatt aaatttctat cttagaaatt
     3661 taatttgtaa aaaaattttc tatatcataa tcaagtatct tagatagttt ataggtaaca
     3721 tatcaactct aattgaatat tcccctttcc tgtacttaaa ataaattgat gcatttttaa
     3781 aacctaactc atttgcaaca ctttgtaagt ataaatttta tgtatgtctt cttttttata
     3841 aaatcatagt gtatcttttt catatattca cattaattgc tatatttcaa taacaatatt
     3901 tccaaaacag aaacttttcg agataattat gtatggatgt aaatcaaagg attaaatata
     3961 ttagggaaaa tatgaatagt tctcaaaaag aattgcttga aaaaacaaat ataaatacaa
     4021 gtgttatgaa tagaatagag tctggtgaaa gaccaacaag agacgatggg ttaataatat
     4081 ttgctaaaat ttttggttta tatactgatt atattcttgg tttatcagat acagaaaaac
     4141 taaatattgg caattaaagc tgaattaatt gtattatatg tgtgatgaat ataaatagag
     4201 aattttttga aaagcttgaa ataacttaga aaaaataaga ggttcactca agaacaagtt
     4261 gctaaagatt aaaaaacaac aaaggtatct actaaaagat atgaaaatag tttaagagag
     4321 ttgagtctgg aaatatttat gtgtattctt taaataaaaa tttatttttt aaatgataat
     4381 taaattttta ctgatatggc tatgctttta aaagtcctaa ccttattttg atattatatc
     4441 tgtctttaca taccacttaa ttaatataaa acagtataaa aattagttac agatgtatct
     4501 tttttgttct ttacatacca cttagttaat ataaaactat taattttcct atgcttgtac
     4561 tcatactcaa aaagctttac ataccactta gttaatataa aacgattgtt attttacgaa
     4621 aggtacggtc gtaatttacg actttacata ccacttagtt aatataaaac aagctagcgg
     4681 ggatagacaa tataaatatc aacacttctt tacataccac ttagttaata taaaacccca
     4741 aaataaattg agcatttcca agacttacat ctatacaatt ctctcaaatt tgcagtgaac
     4801 cacgagtagt gcaaatgata atatttatca tataccctca atgccttatg tttcaactgt
     4861 taagccctat tttatcaaaa atatcgctca ctgcaaaact atttataata ttatgaatat
     4921 attattttta aatatctgta ccaatttgta gtataataaa atcaagaaaa taatttactc
     4981 ctataaagag tgattatttt taagtgaaaa tattattttt ttaaatcatt cttattggcg
     5041 tttgggagat ttttatttta tcataaatat aaactgatat aacaccagct gacatgcttg
     5101 ccactttaga ttattattct tgctacaact attataacat ataaaactta cttattttta
     5161 ccaaatattt tatgtaaaca ataaatatta acttatgaaa tatctgctca tttacagata
     5221 tttaataagt taatagctct ataataaaat ttttatcatt gtttataaaa aattttactt
     5281 tgcatttttt tataaacaac agttttattt ataatgtttt gtggtataat aaaagcaaga
     5341 agaactacaa tctattttgc gttagagtgg agttcatcat aaacagagtt tattttttgg
     5401 atttaaatat aaatttaaat tcaactcgta agtcactctt tgtgcgagag tggctttttg
     5461 cttttttgaa tagtttacta attaagtaaa ctaccacact agctgttaaa ctagctaaaa
     5521 cattaaacaa aaagttatcc atgaactcac ctcccttcga atcgttggga ggataatctt
     5581 ttgtacatga actccactct atagattgta agttaaatct tcttgctaaa aatattataa
     5641 catattatgg taaattatat aattaaaaac acttgtttaa aagttctaat tttaattata
     5701 aactttccca gtttctatat ctataaaaaa ccaaccaaaa gtaccattac tattttttga
     5761 attgatttta acagcataga cattaccact atatatatca tctctaaaat ctatcatatc
     5821 aactactaag ttattgatac tgactttatg tctcttagca cattctttta atgctatctg
     5881 ttttgcttgt tcctctgtta tacttccttt atttttataa tcatctggat tacatacttt
     5941 catattacca ttaacatcat atgtataaac tttgctcata tcatttttag ctacacagta
     6001 tctaacatca ccctctgcac cctgtttaat gtaagcaaat acatagtact cttttcctga
     6061 aaaatcaaaa tttttatcta gtagaaatat taaatcttca tcttctgaat aattaggttc
     6121 gtaataaaat ttcttcacac ctaatagttt tttcactgca ttagcagcat cttctactgt
     6181 atattctctt ccagtattag aatcgcctat attttttgtt acatttaaac actgctgtaa
     6241 tatactatca ctcaaatttc ctatagcagt taatttagtt gtacttttca atacactttt
     6301 attactatca ttatcaacta acacaatagc attattttta gcaacagtag aaccaacaag
     6361 agcatacact aaatcagtac cacttgctat ataaaattct tttactccac tataaaattt
     6421 atttattatc tttttgttag tatcatatct atctgtccca cctaatctag ttgaattagt
     6481 atccttaact attttgtcat tcattgatga agtaccacct attacataac tttctacacc
     6541 agttgtatta aatggaacac tttttccatc agttaatact atagctgctt tatctctaac
     6601 tgctacaggt gcagcactca ttgcatcagg ctctccttta aatgcattag tcaatatgac
     6661 tttatttact ttgttaattg agtttatttc ttttgcgaca ttatagcttg tcttaattct
     6721 atcactacct tgaagtcttt taatctctat tcctttgcct tttagaacag tttcagtata
     6781 tttatcaata gagttttctc ctccaattat atatactttt tttgcctttt ctaatctttt
     6841 aagtgtagca tttggtatgt tatttttctt agtcaaaaga attggagcgt ttatagctcc
     6901 tgcaagtcca cttgcactta atccatcagc cattgtactg tctgcattaa ttaatatagc
     6961 tgtagtatag ttttgtttgt ctgcaattat accagcagtt tcgtatttat ctgcaccttt
     7021 aattgattca attttatcaa gagcatttac agatataggg caactagcaa taaatataga
     7081 taaagataac cctattgtta ataatttttt aattttcata atatctcctt ctcttaaata
     7141 ttttcaccta aattatataa tacttttcag gaaaatttta ccataaattt aaaaggttat
     7201 tattacttaa tataatatta atgaaaaata tcactcttaa attaatgact ttggtagaat
     7261 atggtgataa aataaaatta atgagagaca atataagggg gattatatta tgataatcag
     7321 taaaaaaata acagcaatat tagtatcatt tttaattatg atatcaatgg ttggatgtgc
     7381 tccaaaagaa agtgctacag aagtagctaa aaagtacctt gatagtatag aaatcaaaga
     7441 ctacgaagta gcatataatt tattaagtaa agacagtaag gaaagtgtca gtctagaaaa
     7501 atttgaagaa tatcaagata ctttatttaa acttcaaacc ctaaaagatt ataaacttgg
     7561 tgaagaaaat aaagtagaga aatacaaata ttctaataaa gagtacacaa atcttgttaa
     7621 aataaaagaa actgatacta taaagtatat cactgaaaat aaagaagata ctgcaaattt
     7681 tgatagatat tttataatcg aagataatga atataaatta ttatggcata aagactttaa
     7741 aagagctctt agctctaatt atgtcaaaat cgctaggtca gaaacagaaa aagatgaaga
     7801 taaggattat aataaagctg ctattgatat caaaaaagct atagaaattg atgataaaaa
     7861 tccacttgca tattatacac aatcatatat ttatatagag ttacaacgat atgatgaggc
     7921 tttaaagtca attgataaat acttagaata tctagataaa aataatgaag cttataaaat
     7981 agacgctagt gatgcttaca atttaagagg tgttattttt atgagtaaat cagaatttaa
     8041 taaagctaga gaatcttaca ataaggcgct tgaacttaac ccggataatg aatatgcaaa
     8101 aactaatcta aatgatatta aaggattata aggcattaat attatagaaa ataagtatgt
     8161 caaaagttaa attaactttt gacatgctta ttttctatat tttaaactct attgatttac
     8221 tatataatag tgcataagga gtgtgattac ttattatgag tgttgccata tacttaagaa
     8281 aatcccgtgc tgatgaagag gcagaaaaac aaggcgaatt tgaaacttta agtagacata
     8341 aatcaaccct actaaaacta gcaaaagaac aaaacttaga tgtaattgag ataaaagaag
     8401 aattagtttc tggtgaaagt ataatacata gacccaaaat gcttgaactt ctaaaagaag
     8461 ttgaagaaaa taaatatgat gcagtcttag taatggactt agacagactt ggtcgtggag
     8521 acatgaaaga ccaaggtatc atattagaaa cattcaaaga aagtaagacc aaaatcatca
     8581 cacctagaaa aacatacgac ctaacagatg aatttgatga ggagtattca gaatttgaag
     8641 catttatggc aagaaaagaa ttaaaactta tatcaagaag gatgcaaaga ggtagaataa
     8701 aatcagttga agaagggaat ttcataggaa cctctgctcc atttggatat gatgctgtaa
     8761 caactggtag aaaagaaaga atacttgtac caaataaaga tgctgatgta gttaggacta
     8821 tctttgactt atatattaat gaagatatgg gttgtagtaa aatatcaaaa tacctaaata
     8881 acttaggcat taaaactgct acaggtgcta attggtacaa ttctgcaatt acaaatataa
     8941 taaaaaataa ggcatattgt ggctacattc agtggcaaaa aaaggactac aaaaaatcta
     9001 aaaatccaaa taaaataaaa acagttaaac taagacctaa agatgaatgg attgaggcaa
     9061 aaggaaagca tgaaccttta ataagtgaaa taacatggaa aaaagctcaa aatatactca
     9121 aaaaaaatgg tcatgtcagc tatggtaatc aaataaaaaa tccacttgca ggaatagtga
     9181 tttgcaaaaa ttgtggtaga ccattagtat atagaccata tgcagaccac gactatataa
     9241 tttgttatca tcctggttgc aataaaagtt ctcgatttga atttattgaa gctgccattt
     9301 taaaatcttt ggaagacact gtaaaaaaat atcaattaaa agcatctgat atagacttag
     9361 ataaaaataa taaagatagt aatatagaat ttcaaaaacg agtcttaaag ggcttagaaa
     9421 ctgaattaaa agaattaggc aagcaaaaaa ataagttata tgatttacta gaacgaggaa
     9481 tatatgatga agatacattt attgaaaggt ccaacaatat aagttcaaga actgaagaaa
     9541 ttaaagattc aattaagaca gtaaaaaata gattgagtac tgttaaaaaa gacaattcta
     9601 aaataataga agatattaaa actgtgttat cactttacca tgattcagat tcccttggaa
     9661 aaaataaact cctaaaatct gtaattgata aagcagttta ttataagtct aaagaacaaa
     9721 aactagattc ttttgaatta atggtccatt tgaaattaca tgaagaccag taataccaat
     9781 aacatataaa gagtagtgtg taatgaaata agtcttttag atatacttgg tacagaggct
     9841 aactatgttc ttgatgaagt tgaactaaaa gttcaagttg gaaaacttta cgaacaacta
     9901 aacaaaattc ttactcctag agaaagagaa attgtccagt taagatatgg gttaactcct
     9961 tatggctata agacacaaag agaaattgca caaaaactcg atatatcaag gtcatatgta
    10021 tctagaatcg aaaaaaaagc tcttaaaaaa ctagaaaaag agcttgttca agaaagttaa
    10081 tactatattt taaaaggata tatcgtacat cattagacaa aattttctat ctaaaatcaa
    10141 agttacgata tatccttaaa ttttatagta cataatttat tttatacttt cacatatttc
    10201 cttaaatata ggtgttgcag atttatttcc tttttgagtt ccttctacta acacagttat
    10261 tacatactta ggtctttctt caggataaaa tcctgttatc catccgtggt ctatagcttt
    10321 tttatttaaa ctactttgag ctgtaccagt tttaactcca caaccacctt caatatcttt
    10381 taggtcttta gctgtaccag tcctagatac agacttcata tattctttaa cttgtgtaca
    10441 cacataagga gaaataagtt cttctttttt tgaagactta taagttttta ttgtattcat
    10501 attattgtcc acaagacttt tatataagta tagtggtttg aatgttccat tattagctat
    10561 tatttgagtc atttgattta tttgaagtgg tgtaaattct atattttctt gacctatagc
    10621 taaatttctt attgatatgt tttttggagc ttctctaatt ttttcttcat caagaccaat
    10681 atcaaccttt tcaaataaat gtaatttttc agcagatttg agtatctttt cttttccaag
    10741 tttcatagct atatctaaaa atgcaggatt gcatgaatta gaaaaagctt gtcttaagtt
    10801 ttggaaacca tgaccatctc ttttattaca tcgcagtatt tcattagtat tcccaacttt
    10861 agtcttacct gtacagttgt aagtatagtt ctcatctata acaccatttt caagtgcaga
    10921 aaataaaact accatcttaa atacagaccc tggtggatat gtcgctttta taactctatt
    10981 ttcaaattct ccattttttc cttttaaact ttttgatatg tcattttggt caaaattagg
    11041 tctagagcac attgcaagaa tttcacctgt agatgcttcc gagattattg ctgctgtagg
    11101 attttcttct ttatttagta tttgttctaa ttttttttga atgttataat caatagtagt
    11161 ttttagatgt ctatctttat ccttattatc aacagttttt acacttccct ttagtatgtt
    11221 tagagatttt tcatttcctg catctccagc tttaaaagca cttatatact tttcatttga
    11281 atttctaagt acactatcca tactcttttc aattcctgat tgacctgatt tatcatcttc
    11341 ttttatatat cctattgtat gtgataataa accatctttt gcgtacctca tagttttttc
    11401 ttctaccatt attccatttt tctcaagttc ttttttcata ctcttatcga tatcgatatt
    11461 tttagtctga atctgtatta tagggcgtgt caattgctct tgtacagctt tatatatatc
    11521 taaatcattc atcttagtag ctttcttaat aagctccaaa attttatagt tattatttaa
    11581 tttttctttt tcaacaatta atacctgaga tttacttgta tctgtaagct tcttgttgtt
    11641 tctatcatag attattcctc ttccactatt taactcgact ttctcaacgc tttgtgattc
    11701 aacactttgt ttgtatttat caccttttaa tacttgtata tcaactagcc tataaattaa
    11761 aacactataa attattaaaa ttatagtaaa tatacaccag cttctctttc ccactttttt
    11821 caaaaatgga gtcttctttt ttgacatgaa atcacccctt agataatttc attattgaca
    11881 atttctaagg ggctaatact atttttatca attttttatt attcttcaac cttttcttct
    11941 ataacttttc taagcatata atctttacca acttttactg atagctttaa cttcactatt
    12001 tgtctaggat gaggagctga ttctatagct tctccttctt cattatacat ttcttcaatt
    12061 ttagtaaaca tagtttcttt atatggtccc ataacttcta tttcatcacc aacacacatt
    12121 ttatttcttt gctcaacaac aataagacca tcttcatctt ctgtttctct aactatacct
    12181 ataaaatcat agtttctaac atatgatgct gattgataat tttggtcttc tgttgttggt
    12241 ttattcaagt aaaaacctga tgtaaaatgt ctatgactac ctttttttag ttcttccatc
    12301 cacattggat taaacttcca gttttctggg tctctgtaaa attcatctat tgccattctg
    12361 tatgctctta caacagtagc tacatagtat gcagtcttca ttctaccttc tatttttaaa
    12421 cttgttatac cagatttgat taactctggt atatactcta tcatacataa atcttttgag
    12481 ttaaagaaga atgtacccct ttcatcttcg tatattggaa aatactcacc aggtcttttt
    12541 tcttccacca aatgatattg ccatctacaa gattgagcac aagaaccttt gtttgcatct
    12601 cttccagtca tatagttgct tattaaacat ctgccagaat aagaaataca catagcacca
    12661 tgcataaacg cttctatatc catatctaaa ggtgtttttg ctcttatctc atatatttca
    12721 tcaaaagata attctcttgc cactacaact ctttttattc catgctcgta ccagaagttt
    12781 gcacttagat aattggttgt atttgcttgt gtacttaaat gtatttccat attcggaatt
    12841 acttttttaa tgacactaag cactcctggg tcagctatta taactgcatc tattcctatc
    12901 tcttctagtt ccaacaaata gtcttctaat tgtagaaaat cttcattatg aggtattata
    12961 tttactgtta cgaatacttt cttgcctctt tcatgtgcaa aagctactcc ctctgccata
    13021 tcttccttac taaaattctt cgctgcagac ctcattccaa aaatttctcc acctatataa
    13081 acggcatctg ctccatatgt tatagctatt ttcaatctct ccaaatcacc tgctggagct
    13141 aaaagttcta ctttatttaa ttccatctac atcacctctt ttactttctt tataacttag
    13201 tgctacacca tcacctattg gtaccaatga tgtgcttaaa tagtcacaat tacatatata
    13261 atctaaatac tttctcattc ttttaactat agtctttttt cttcttacaa caaaatcatc
    13321 atgagcaacc atacctttat acaaaatatt gtcagatact aaaagtcccc catcttttaa
    13381 tttatctata actaaatcaa aaaatagctt atattgacct ttggctgcat ctataaatat
    13441 aaggtcaaat tcaccttgga cttgactaag tttttcttct gcatctcctt ctagtatagt
    13501 tatgttattg tcaaatccag ctaattttat gttttctttt gctttttcta tcattttttc
    13561 atttctctca acagttatta tatctgcatc ttttcctata atagttgcaa aaaaaataga
    13621 agaataacca attgcacatc ctacttctaa aattctcttt ggtttttgaa cctttaacaa
    13681 gacttttaat aaatctgaaa cttctttatg tattatagga acactatttt cctctgcata
    13741 aatttctaaa tcttttaaaa atccttcttt ttcttttaat gtcactctta tataatcttc
    13801 aaccaaatca ttaactatat tactcataaa aacactccct aaaataataa atatccaagt
    13861 tttatattaa tttattattt tttaaattca tattgttata tatatttaca ctttctttat
    13921 tataaatcta agaataacaa ttttcaataa aaaagcccaa cattattctt accctgttag
    13981 actttttata atcatggttt atttttttgt attttcttta tttgttgctt ttgtatcttt
    14041 tgattgttta tcccttgctt ctttatattc tttaacgtat tttaaatggt cttgatagtt
    14101 tgtactataa ttatttccac catcaatctt agctacaaaa tataaatagt ctgttttctc
    14161 tggatataat gctgcttcaa tagattttat accaggattt gatattggtg taggaggtaa
    14221 tcccttattt ttataactat tatatggtga atcaattttt aaatcatcat atgttacaat
    14281 tttctttctc tcttcaaata tatattgtat tgtagcatct gattgaagag gcatacctac
    14341 cttaagtcta ttataaaaca cacttgctat tataggtctg tctttgtcaa gaacagcttc
    14401 tttctcaatt attgatgcca tttccattac ttcttgaaga gtcatattta attctttttg
    14461 tttcttttta aacttgtccg tgtatttact gtcaaatact tttaacattt cgcttaaaat
    14521 atcttcttct gattgtttct ttgaattaaa ataataagtt tctggataca agaacccttc
    14581 taaagaggtt attccatctt ccttcaaata tgaaaattta tcataaaatt cttgaggttt
    14641 ttttattaaa ttttcaaaag tagctttatc tcctaaattt ttacttacta acattgctat
    14701 aatttcttta gatgtagctc cctctggaat agtcactttt ataccatcct gatatatttt
    14761 acctgaaact aaaagactta ttatatcatt gtttgaatac gattgattaa gtaagtatgt
    14821 tcctgatttt atagacggag ctttattgct aacctttacc aaaagtttaa ataatagttc
    14881 attctttatt aatttatttt catataatat gtcagatatt tttccaacag atgctccact
    14941 tggaacgtcg ataattacat cttttttgtt atttttatca taaggaccta tttgtataaa
    15001 cacaaatata cctgctaaga taatcaaaat aacaattatt aatactgcta ttttcaatct
    15061 attttcttta aagttcataa tataatctac tccttatgta tatttatcca ttttctatta
    15121 taaaactaga tactttaatt ttc
//
LOCUS       DAMJZE010000097        14545 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJZE010000097 DAMJZE010000000
VERSION     DAMJZE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.098"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            116..1744
                     /gene="aspD"
                     /locus_tag="PC541_002443"
     CDS             116..1744
                     /gene="aspD"
                     /locus_tag="PC541_002443"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2839902.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1865..2860
                     /locus_tag="PC541_002444"
     CDS             1865..2860
                     /locus_tag="PC541_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2839903.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3038..3421)
                     /locus_tag="PC541_002445"
                     /pseudo
     CDS             complement(3038..3421)
                     /locus_tag="PC541_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3648..3745
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3886..4444
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3886..3915
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4730..4888)
                     /locus_tag="PC541_002446"
     CDS             complement(4730..4888)
                     /locus_tag="PC541_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839904.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5182..6552)
                     /locus_tag="PC541_002447"
     CDS             complement(5182..6552)
                     /locus_tag="PC541_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2839905.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6768..7885
                     /locus_tag="PC541_002448"
                     /pseudo
     CDS             6768..7885
                     /locus_tag="PC541_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(8248..8328)
                     /locus_tag="PC541_002449"
                     /pseudo
     CDS             complement(8248..8328)
                     /locus_tag="PC541_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8368..9036)
                     /locus_tag="PC541_002450"
     CDS             complement(8368..9036)
                     /locus_tag="PC541_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2839906.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9889..12309)
                     /locus_tag="PC541_002451"
     CDS             complement(9889..12309)
                     /locus_tag="PC541_002451"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2839907.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12663..13013)
                     /gene="rsfS"
                     /locus_tag="PC541_002452"
     CDS             complement(12663..13013)
                     /gene="rsfS"
                     /locus_tag="PC541_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2839908.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13130..13702)
                     /gene="yqeK"
                     /locus_tag="PC541_002453"
     CDS             complement(13130..13702)
                     /gene="yqeK"
                     /locus_tag="PC541_002453"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2839909.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13703..14392)
                     /locus_tag="PC541_002454"
     CDS             complement(13703..14392)
                     /locus_tag="PC541_002454"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2839910.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4904 a         2379 c         1839 g         5423 t
ORIGIN      
        1 taatttcata ctttactttt tctataaacc tattataatt aaagtataat catttttgca
       61 acttgtttat tttattataa aaatgtgaaa cttattacat aaggaggaat ttaacatgaa
      121 aaatcatact tataaagaaa tcaaaaacat atatggaaaa ataagccctt ttgaatttaa
      181 ggacaaatta atagatattg caaaatacac tgcaaaagaa aataacagag aattacttga
      241 tgcaggtcgt ggaaatccaa actggacttg ttcaactgct agagaagcct tttttacttt
      301 tggtcatttc gctattactg aaactcgttc taattgggat ttaggtcatt tggctggaat
      361 gcctcaaaaa aagggtatta aagaaagatt ttttaaattt ataaatgaaa atatagatat
      421 gcctggagca tatttagcta gagatattat aaactttggt ataaatgaac ttggatttga
      481 tggagatgaa tttgttcatg aactagctga tggaattatt ggtgataact atccacttcc
      541 agatagaatg ttacctcata tggaaaaaat agtacacgat tacctagttc aagaaatgaa
      601 atatgatata tcaggtaaat atggtgatgt agaaattttt gctgttgaag gtgctacagc
      661 tgctatgtgc tatatatttg attcattaat ggcaaatgaa cttctaaaaa aaggagatac
      721 catagcctta atgactccaa tatttacacc ttatcttgaa atacctaacc ttcctcgtta
      781 cgactttaaa gtggtaaata taaatgctaa tgaagttgat gaaaaaggtg ctcatacatg
      841 gcaatataca aaaaaagaat tagagaaact tcgtgacaaa tctattaaag ctttattcgt
      901 tgttaatcct aataatcctg cttctatagc tatggatgaa acatcttgta ataatctaat
      961 agatgtaatt gaaaattata ataaagattt aatgataata tcagatgatg tatatggaac
     1021 ttttgtagaa gatttttcat ccttaatgtc aaaactacct tacaacactg ttggagtata
     1081 ttcttattca aaatattttg gagttacagg atggagactt ggtacatttg cccttcataa
     1141 gaaaaatgtt tttgataaga aaataaatga tttaactgga gaattaaaaa aatcagtaga
     1201 taagcgttac agtgatatga gtcttaaccc ttctagcctt tcatttatgg aaagagttgt
     1261 tgctgatagc agacttgttg ctctcaacca tactgctggt ctttctactc cacagcaagt
     1321 acaaatggcg tttttctctg cttttgcatt gattgacaaa gtagatgcct acaaaaaact
     1381 caatatgagt atctgccata gacgtcaaaa actattattt gaagctttag agcttccaat
     1441 taatgaaaac aaaaacaatg ctgcatatta cactcaattt gatattgaag aatgggctaa
     1501 attaaactac ggagaagact tatttaaatt tataagtaaa actgcatctc cagtagatgt
     1561 tctttataaa ttagctaatg actattcagt agttttactt agtggaaatg gattctatgg
     1621 tcctgaatgg tcaattagaa tatctctagc aaatctatat gatgaagctt atactaagat
     1681 aggaaaagcc ataagagaga tattaaatgg ttatatcgtt gaatggcaaa aaattaagaa
     1741 ataattatat tacactgaaa tatctaaatt atatgaataa aagagtttat gtagacctta
     1801 actacttttt ttgatataat cctatcggaa ttataaattt tatttatgaa aggcagagaa
     1861 tagtatgagt attaagaaaa aagttgccat agtttttact ggtggaacta tatctatgac
     1921 agtagatgat aaagtcggag cagctatacc aactttatca ggtgaacaaa tactatcaat
     1981 ggttaccaac attgataaag ttgcagatgt tgaagttttt aattttgatg aaattccagg
     2041 acctcacata acaccttata gaatgatgga attgaaaaat tatgtaaatg atttattagc
     2101 aagaaaagat attacaggtg ttgtaataac acatggtaca gatagtttag aggaaacagc
     2161 ttattttcta gaccttacaa ttgacaacat caaacctgta atagtcacag gtgctatgag
     2221 aagtagttct gaacttggat atgatggttc aagtaattta tctgcatcag tttgtactgc
     2281 agtatccaaa gatgctatgg gaaaaggagt ccttgtagtt ttaaacaatg aagtattatt
     2341 agcttctgaa gctactaaaa caaatactct ttctctaaat acatttaaat ctctaactag
     2401 tggcccatta ggaataattg actgcaacga acttgtcctt acaagagata ttgtaaatag
     2461 aacaattata gatactgata aggtagaatc taaagttgcc ttatttaaag catatgtagg
     2521 agaaaatgct gactttataa gatttgctgt tgatagtggt tataaaggta tagttattga
     2581 ggcaatgggt agaggtaata ttcctcctca aatgctttct ggagttgaat atgctagaga
     2641 aaaaggactt cctgtagtta tagtttctag atgtcattca ggtagagtat tcgatagtta
     2701 tggttacttt ggttctggta gagatttaaa aaatcttggt tgtatatttg gtggggactt
     2761 accaggtcaa aaagctagaa taaaacttat cttagcactt ggaaaaactg ataaccttga
     2821 tgaaatcaaa gatttctttg aaaaaggaat ctattgttaa ttctaataac ttctatttaa
     2881 ttatttattg atatttcaac atttttaaat caaaatctaa tacttatgtc agttaaatgt
     2941 cagcaaaata ttttatccac aagttttcca caacttatcc ccaatattat ccacaagaaa
     3001 aaaggtagca cttatattag tagctacctt ttatttatta atcttcatct ttccagcttt
     3061 ttatccaatt tcctatttct attatttttt cttcagttat ttcttctcca tcatgcaatt
     3121 ttgaaattaa ttctgataat ggattgttat gtagacctcc aaaaattctt tttccctcta
     3181 ttttgagata ttctttctct tttattaata ctctatagtg tacatgtttc ccaattctct
     3241 gcgatatgaa aaaacctttt ttagttaatc tagctaaaag tgtaattgta gttgtatgct
     3301 tccaagcata tgttttttct atttcatctg caacatcttt tgatattaca gttttataac
     3361 cagtattcta tatgtatttc ataattttta attctgcatc tggtaatttt ttattcaaca
     3421 taatatattc cttctttcaa tttatgtcac ttcctcaaac ttatctagct taaattaata
     3481 ataggagctt tttatcacaa aaactacagg taaatttttc tatatattct acgtatttag
     3541 tctgtttttt aagtgtgaaa ttacgttcat acctataaat ttattcaatt ttcaaacaat
     3601 cttattgaat aataactaat tctattgtat tatttagtta tagaataaaa ctaaatcggc
     3661 aaaactagag aaatctagtg acgcaaagct atagagacta agacttatat aaatatctta
     3721 tgagttatgt cagccagttg ccaaagagat attgttcttt ttgtttttat gaaagttttt
     3781 tagggggata ataatttata tatttagaga taaaatagat aaatgtacac atatattgac
     3841 tgcttatatt agcagttcgt atgattattg taattttttg gatacaattt acattccata
     3901 tagttaatat aaaacaagtg tttcgaattt cttttctgaa actataccaa caatttacat
     3961 tccatatagt taatataaaa cgttatatac tgaaagcggt gaaactggtt acgtagcaat
     4021 ttacattcca tatagttaat ataaaacctg tgcgtgtagg agatggtaac acaactgttg
     4081 aagaatttac attccatata gttaatataa aacacacatc tacatgtttt tctatctgag
     4141 ttcatctcta atttacattc catatagtta atataaaact ttttaatcct tcttctgaac
     4201 tcccaaagtt tatatcattt acattccata tagttaatat aaaacccacg aaatttaagg
     4261 tcgatatatc agatttgaaa aaatttacat tccatatagt taatataaaa caagctaaat
     4321 ggagcaaccg accaagtcgc tagaaataat ttacattcca tatagttaat ataaaacttt
     4381 tctattaagt gtaagcagaa tgcatggaat atgaaattta cattccatat agttaatata
     4441 aaaccccaaa ataaattgag tatttccaat gcctacacat atacaaatct ctcaaatttg
     4501 cagtgaacca tgagtagtgc aattgataac acttatcaca agtcctcaat gccttatatt
     4561 tcaactgtta agccttaatt tcttcaaaaa atcgaacact gcaaaatttc tatgacttta
     4621 ttatatcata aaaatattat ttttgaatat ctgtaccaat ttgtgatata ataaaagcaa
     4681 gaagaactac aatctatttt gctgtagagt ggagttcata atttagaaat tacttagttt
     4741 tatggaatct gatttttaaa tcaaattccc aaccactctt agttgccgct ttgagtggtt
     4801 ttttacgctt tctaaatagt ttgctagcta tataaactat taagctagca gatagacttg
     4861 ctagtatgcc ttgtaaaaaa ttatccatgt atattcacct cccttcttaa cgtcgggagg
     4921 ataatttttt gatacatgaa ctccactcta taaattgtag attacatctt cttgctaaaa
     4981 atattataac atataattct tacatatttt acctatttat tactttttac attataataa
     5041 tattctttta atcaaaaaaa cacctactat taaagtaaat gtttttaagt atttttaatt
     5101 ttaaaatcca tatagttcat atgaaactaa atataataac tcaacatatg agcaatatcg
     5161 agacatttac atacccacta gttatttata ttcaaaaaaa tcttctccat ccgggttata
     5221 aaataatatt ctacttgcca atccttgttc atatttataa ttttctttat cacctacata
     5281 agaaaatgtt aatgtcatgc ttcttgatgg atctaatgaa attatatcta aataaatagt
     5341 acatttttct cctggcttta atacaccgaa actattaaaa tccattttta ttttagcgta
     5401 ttgccccata tataatgttg aattagcttc tccataaact tttaattttg ttacatctaa
     5461 attttgtgta cttgtatttt ctatatttat aggaactgtt gggtctttat atttttcagt
     5521 ataatattca aggttatcat cataacattc aagataactt cctttatctt gccaaattat
     5581 gtgatttgaa tcaaaatcaa tctttaatcc acatattata tttttatttg aagatggaag
     5641 actaatatta ttgatggtat tttcacactc tagcataatt ttatcactta gatttcctat
     5701 cccggttact tttgcagcat ttttaagtat atctttatta cttccaatat caactagtac
     5761 aataggatta tttttagcca ttggtgaagc aactaaagca taaactaaat cagtaccact
     5821 agctatataa aattcttttg cgccattata gaacttattt actatttttt tattagtatc
     5881 atatctatct actccaccca atcgagttga attagtatca tttactaaac tatcactcat
     5941 tgatgaagtg ccacctattg cataactttc tataccagtt gtattaaatg gaacactttt
     6001 tccatcagtt aatactatag ctgctttatc tctaactgct acaggtgcag cactcatagc
     6061 atctggctca cctttgaaag cgttagttaa tattacttta tttactttat taatggaatt
     6121 tatttctttt gctacattat aacttgtctt aatcctatca ttaccttgaa gccttctagt
     6181 ctctattcct ttatctttta gaaaagtttc tgttgcttta tcaatggaac tttctcctcc
     6241 tattatgtat acctttttag ctttttctac tcttttaaga gttgcattag gtatattatt
     6301 ctttttagtc aaaaggattg gtgcatttgt tgctcctgca agtccactcg cacttagccc
     6361 atcagccatt gtactatctg catttataag tattgcagta gtataacttt gtttgtcagc
     6421 tatgatacca gcagtttcgt atttatctgc tccttgaatt ttatcaattt tatcaagtgc
     6481 attagctgat ataggaaaac cagcaacaaa cattgacact aaccctagtg ataataattt
     6541 ctttaatttc acaatatctc ccccttttat atatgaaata ctatatatct taataattct
     6601 acattaaatt ttcttttcct cctttaacga tatttatctt cctgttaatt taagtgtata
     6661 caaatacgtg ttataatgta actgtaaaga attaaaaaga taatgacata attagagtag
     6721 tacaactgtt cttttataaa actaacttat aaagggagcg tacatatatg aatatcaaat
     6781 cagcttttat aagaaaaaga ggggaaaaat tctatgtata tgtggagtac atagaggaaa
     6841 caactggcaa gataaaacag aaaagttatg gaagttatga aaagaaaaag gatgctgaaa
     6901 aacatttaat tgaaataaaa tctactataa acaataataa gtttgttgct ccaaaagata
     6961 tgacatttgt agaaagatgt tataaataca tggacatgaa taaaaataat tggtctccat
     7021 atactgttac aaatacaaaa tcttgggtta aaaattacat agaacctctt tttaagaatc
     7081 taaaactcac agaagtaaat cctagttcag tacaagcttt tgtaaattat aactttaata
     7141 attctacctt tactagtgct aaagtaaggt atagctttct aagttctgta ataagggaag
     7201 catatagatt aagagaaata caagaaaatt catgtgattt tataaaactt ccttctaaag
     7261 aaaaatattc tacaaatgaa atatacaata gaaaagaagt attattgcta atagaaaagt
     7321 tacttagtaa taatatagaa atacctattc ttttaatgtt acttctaggt ttaagaatgg
     7381 gagaagctac tgggcttagg tggtcagatg ttgacttaga taacaatctg ataaatgtta
     7441 atcaaattct tgtctattct aatggcaaaa tatcttttaa agagccaaaa acaccaaaat
     7501 caaaaagagc tttatcagct ccaaaagaat taattgaaaa attgaaaatt aaaaaactaa
     7561 gacaaagcaa attaaaattg taaggtatcc tggaaaacag aaataattta gtttgtctaa
     7621 acaccaattt aaatccgttt ctaccttctt cactaagaaa aacgtttcac aattttacaa
     7681 aaaaaataat ttaaaaaaga ttagaataca tgatttaaga tatacaagtg ccactctgct
     7741 gcttttaggg ggaactaata tgaaagttgt ttctgaaaga ttgggtcata cagatataaa
     7801 aataactatg aatagatatt ctcatgtact taaagaaatg gacaaagaag cttccgacaa
     7861 tctaagcgaa cttctattta aatagtaaaa actgactgtc agttaaatgt cagttaaacc
     7921 taaaaaaata ggttttgtca gttgattatc agtcaattat tttataatag tttcacttaa
     7981 aaacgtctat atctacacat tttactattt gtttatgctt attaatatct atctgttatt
     8041 attaattcta atagcttcta tttaattatt tagttcagtt tacaggaaac tacacacaaa
     8101 ccataaaata ggatgttgac aatataacag atagtatgct cctgtaaaag gtaacgtact
     8161 atctgttatg ttgtctggat ttccaaatgg ttaccctttg gcacacaact ttgctatctc
     8221 cttggtaaac taaaagtttg taatctaaaa tcctaatcca tctctataaa atttaattga
     8281 cttctccata ctttttacac ctaaacatat aaaagtaatt ttattcactt aatgacctcc
     8341 ataattccca aattagctat ttggaactta actaaaaaat cctaatataa gggcaactat
     8401 tataatggca atcggaatac atactgtgta atcctgttcc atagagtcat catctgaatg
     8461 tagttttaca attcgcaatt tagcaatcag tttgaagcag aaatagccta ttattgttcc
     8521 aattacattc gtgagtaagt cgtctatatc ggttgctctg tgtaacgtaa aaagctgaat
     8581 agtttcgata aaaagagata atccaaaacc aatcaagaat gtatttctca aacgttcaaa
     8641 ggttttgctg ataagcggac ataaaaaacc taatgggaca aaaagtaaaa tattcaaaat
     8701 aaaactcaaa ctaaatccat cactaaaagg aataagattt atgtttggat taaagaacgt
     8761 ttcccctaac tgtgacagtc ttatatattc actgaatgta gggataccaa caatatgcga
     8821 tagtgttaaa cataagtagt aatacattgc cagtgataaa atagatgttt ttacctttgg
     8881 aatatcacta tttttcttga atattacaac taagaaaata ccaatcaaga taattcccaa
     8941 caagaaaata ggattaatta acaataaatc tttgatagct tcaaacagac tgagttgaat
     9001 ttctacacct gattgattat ttgttacagt ctccatttgt acatacctcc tcataacatt
     9061 tcaattattt ctaaaacaca aaatagaaga taaatcttaa aatcatccta tcaaaatttt
     9121 taaatttgcc tttatcagtt tataccacca gttaattcct gtcttacttc tgtatggtat
     9181 tcttgggatt ctttcatcaa attagacaaa aaatggttac catatcaata aactggttac
     9241 gctccatatc tcccgaagca accatcccct gagaataaat ccgtatcagg aatctccaca
     9301 tctactgcac ctatcacaag ttatttgata acatcctcag acaatcccaa cttggatata
     9361 tcgacaacac ccattagaat accactgctg accatacttg ttttcctgct gataatttta
     9421 atcaactggg aagttgaaac gctatctttt ttacaagttc ctttttagaa acatggtagc
     9481 tattatatga atctgaattt cgttgtctgg gtgtttcata taaagcatag atacgcacct
     9541 tttttgcctg ttttcattat atagaaaaaa atttcaaaat acaataatta tatttttttc
     9601 gtttaagctt agagcagttc cttgatgaac aacactttgg aaactgaatg atttacatac
     9661 cacttaatac attaactcga tttaacatta attataataa tatttaaaac aacaactagg
     9721 gtatataaga atgaactttt tagttcttta atcatttttt tgtataaaat caaatctatt
     9781 tttataattt caacaatttt ataatttcaa caatgaaaaa aggcttatct ctattttgag
     9841 acagcctcgt ttattttata aatttatatt tttgttcata tttttgagtt atttaacaac
     9901 tatatttact agttttttag gtacagctac aactttaact atagttttac cttcaactaa
     9961 tccttttatt ttttcatctt ctaaagctac cttttccatc tcatctttag atatatttga
    10021 gttaacagtt aattttcctc taacttttcc attaacttgt acaacaactt ctatttcatc
    10081 ttttactaaa gctttttcgt catatttagg ccagtctatg tcaaatacac ttccttcttt
    10141 acctatcata gtccatagtt cttcacctaa atgaggggca aatggtgcaa ttatagttac
    10201 tatagtttgt acaccttctt ttataactgc ttcgtttata ttatctaact ctttatattt
    10261 atacatatca tttattaatt ccataagagc tgatatagca gtattaaatc cgaatttttc
    10321 acttaaatca gcagtaactt ttttaagtgt agagtgtata gtatatctca tatccttatc
    10381 ttgggaattt aattctccaa actctacatc ctttttaacc acatctgcta actcgtcaac
    10441 caatctataa accctattta agaatctaaa gcatccatca acaccttgct ctgaccagtc
    10501 caaatctctt tctggtggtg ctgcaaataa tacgaaaagt cttgcagtat ctgctccata
    10561 ctcatctatt atatccatag gagatactgt attcccttta gacttactca ttttagaacc
    10621 atccataagt accattcctt gagtaagtaa gtttttaaat ggctcattga agtctaccat
    10681 tcccatactc ttaaatgcct ttacaaacca tcttgcataa agtaaatgca ttatagcatg
    10741 ttctactcca cctatatatt ggtctactgg atgccatcta ttaactaatt ccttactaaa
    10801 tggttcattt tcattattgc tatccacata tcttaagaaa taccaagaag aatcaacgaa
    10861 tgtatccata gtatcaactt cccttctagc tggttttcca cagtgaggac aagttgttga
    10921 cataaattgc tttgaagtcg taagtggaga ttcccctttt ccagtaaatt caacatctgt
    10981 tggtaaaaga actggtaaat cttccttttt aactggaact attccacatt catcacaata
    11041 aaccacaggt ataggacaac cccagtatct ttgtcttgaa actaaccagt ctctaagtct
    11101 ataattaatt gttctctttc ctctaccttc tttttctaac ttatcgatta ttccttcaaa
    11161 tccttctgaa gcatctaatc cattaaattc ttctgaattt atcattttgt tatcttctgt
    11221 tattacatca attatgtcta aattatattt ttcagcaaaa tctctatctc tttcatcatg
    11281 tgctggaact gccattatag cacctgttcc atactcaact aatacatagt tagctatcca
    11341 tagaggaact ttcttgccat ttaaaggatt tataacatat ctacctataa acattccttc
    11401 tttttctaca tcagctgctg ttctttctat ttctgtcata gtatgcattt tttgaacaaa
    11461 agcttcaaca tctgcttcat attcagtccc tgcaaccaat tctttaacta attcatgctc
    11521 tggagcaagt accatataag taactccata agttgtgtct ggtctagttg taaatactgt
    11581 catagattta tcagttccat ctatatcaaa aactaaatct gctcctgtac ttttacctat
    11641 ccaatttttt tgcattgttt taactttttc tggccatcca tctaatatat ctaagtcatt
    11701 aagtaattct tctgcaaatt cagtagtctt aaaataccat tgttctaaat ctttctttaa
    11761 tactgttgct ttacatctct cacatgctcc ttgaacaact tgctcatttg caagaacagt
    11821 ctcacaagaa ggacaccagt taacatatga ttttttctta tatgcaagcc catgctctaa
    11881 aaacttcaaa aatatttctt gtgtaaatct atagtattct ggcgttgaag ttgcaacttc
    11941 tttatcccaa tcataactaa tacctaataa atttaattgc tctttcattt cttctatatt
    12001 ttccattgtc catttatgtg gatgtattcc atgttttata gctgcatttt cagctggtaa
    12061 tccaaaagaa tcccatccca ttggatgaag tacattatat ccttccattt ttttgaatct
    12121 tgcgacaaca tcacctatag aataatttct aacatgtccc atgtgtattt ttccagatgg
    12181 ataagggaac atttctaatg tgtaataatt aggtttttca gtttgtgcag tatccatttt
    12241 atactgatta ttatctttcc agattttttg ccatttcgat tctacttctt taaaattata
    12301 tacgctcata ttttgcctcc tatttttaat tgtgtataac taccttattt agtattattt
    12361 acttataatt tccaaataat tctttgaaaa taaaaaatcc tcgtcactaa acttacgttt
    12421 agggacgagg ataaatcttc gcggtaccac cctaattagc ctactacata tatagactct
    12481 cttagatagc ttttaatcca gccatggaga atagatttta atctagtaag ttcaaagtta
    12541 tataatatta gttctcacct tccactaact ctctgaaaaa atataatctt ttactgctct
    12601 agtctattca atttaatata atttttgaaa ttttctaatt ataataacat atattatcat
    12661 gtttatgcta atgtgtcaat atctatataa ggtgcatctt tccataattt ttctaaatta
    12721 taaaaccctc tattttcctc attaaataca tgaaccatta tgtctccata atcaaggagt
    12781 acccaagttt gagttccttg tccttcttta cctcttggct ctaatcctag ttttgctaat
    12841 tcatcttcca cattgtcagc aatagctttt acctgtctct gagaagatgc tgtagtaata
    12901 ataaaataat cacacaaact agaaactttt cctatattta ttataactgt atcttgaccc
    12961 agtttatctt ctatggcatc atatgctatt tttgtcattt gttccactgt catataaaaa
    13021 ttcctcactt tcctaattaa aatacactta gttattgtaa catattaata cgattttgta
    13081 acatagtcaa atttaaattt attaatatgt ttgcctttat atagcttttt tataataact
    13141 tttctttaat tagatagttt cttgccataa ctgttcttgg atgtatctct tcttttttat
    13201 tgatgacaaa cattaaagta ttattaaaag aagttaaaag tgcttcatca agcttccctc
    13261 catatgctaa ttctcttaat gtatctacaa ctgggaattt tcttccttct tcaattaaat
    13321 cagctatgta aattattttt tctaaaagag acatgtcctc tctccctgtt gtatgatatt
    13381 tgattgcatt tataatatct tcatcttcca cttcaaattc atactcgcat atgtacgccc
    13441 caattacact atgagacaag gaacgatttc catcctctat tgggtctaaa tatatttcat
    13501 acttatttac ataatcctct atttccttgt cacttaaata ctttgcacaa tcatgaatca
    13561 ttcctgctaa gtaagctttt tctttgtcac atccataaat ctcacataat ttttcagcac
    13621 attttgcaac attttttgaa tggctaagcc tccccactgg tagcatttga tttagtcttt
    13681 ggttaatatt ttctaagttc atttaatcat ctccatattg atatagttta ttttcatata
    13741 aataattttc tacattttcg ggtactaagt aacgtattga cttgccttca tttagctgtt
    13801 cccttatata tgtggacgat atatccagtg atggaacata aactgttata atgtcagcat
    13861 tatacttctt agttagtttt tctatagtct cttgtgacct taacaaacta attcctggtc
    13921 tagttgcggc tataaaagta gctaactcaa agtttttctt tacatctttc cactcttcaa
    13981 cactacaaat tgcatctgca cctgttataa aataaatgtc tgcatttttg tattttttct
    14041 ttaagtattt taatgtatct attgtataag tccttttctt tctttcgatt tctacttttg
    14101 aaactaaaaa atcttcgttg ctttcagttg ctagaagtgt catattatat ctatcatact
    14161 tatctgtagt gatacataat ttatgtggtg gatttcctga tggtataaag attattttgt
    14221 caatgtcata cttgtctcta ataaattctg ctgttgctaa atgagcatag tgtatagggt
    14281 caaaagtacc tcccaatata cctattttga ttttgccttt atggcgatta aatttctcta
    14341 gtttttcagt atttttcaac tctgccatct taactagatt ttcacttctc atattttgtc
    14401 tccttttaat gttataatac tattgtacca ttatttttta tttcttaaag cttataattt
    14461 gtttttttgg gtatataatt atatttatat gctaaaaaac actttaaaaa ataattattt
    14521 aacataattc tgctttactt tttaa
//
LOCUS       DAMJZE010000098        14356 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJZE010000098 DAMJZE010000000
VERSION     DAMJZE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.099"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            114..935
                     /locus_tag="PC541_002455"
     CDS             114..935
                     /locus_tag="PC541_002455"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2839911.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(1011..2399)
                     /locus_tag="PC541_002456"
     CDS             complement(1011..2399)
                     /locus_tag="PC541_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2839912.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(2534..3292)
                     /locus_tag="PC541_002457"
     CDS             complement(2534..3292)
                     /locus_tag="PC541_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2839913.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(3294..4010)
                     /locus_tag="PC541_002458"
     CDS             complement(3294..4010)
                     /locus_tag="PC541_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2839914.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(4012..4719)
                     /locus_tag="PC541_002459"
     CDS             complement(4012..4719)
                     /locus_tag="PC541_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2839915.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(4835..5371)
                     /locus_tag="PC541_002460"
     CDS             complement(4835..5371)
                     /locus_tag="PC541_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2839916.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(5473..6543)
                     /locus_tag="PC541_002461"
     CDS             complement(5473..6543)
                     /locus_tag="PC541_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2839917.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(6536..7594)
                     /locus_tag="PC541_002462"
     CDS             complement(6536..7594)
                     /locus_tag="PC541_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2839918.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(7581..7913)
                     /locus_tag="PC541_002463"
     CDS             complement(7581..7913)
                     /locus_tag="PC541_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2839919.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            8112..9233
                     /locus_tag="PC541_002464"
     CDS             8112..9233
                     /locus_tag="PC541_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2839920.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(9361..11259)
                     /locus_tag="PC541_002465"
     CDS             complement(9361..11259)
                     /locus_tag="PC541_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2839921.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            11574..12350
                     /locus_tag="PC541_002466"
     CDS             11574..12350
                     /locus_tag="PC541_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2839922.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(12442..13194)
                     /gene="xth"
                     /locus_tag="PC541_002467"
     CDS             complement(12442..13194)
                     /gene="xth"
                     /locus_tag="PC541_002467"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2839923.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(13355..14305)
                     /gene="galT"
                     /locus_tag="PC541_002468"
     CDS             complement(13355..14305)
                     /gene="galT"
                     /locus_tag="PC541_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2839924.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT         4857 a         2377 c         1548 g         5574 t
ORIGIN      
        1 tttttacaat tttttatttt ataatctact tttttctttt ttttatagta atatatatgt
       61 atataaatat tttggtatca ttataattaa tacatactaa tggagggttt tttatgcaaa
      121 aaaaagtcgc agcaattaat gacttatctg gaataggaaa atgctcatta tcggttgcta
      181 tacctatact ttcagcatta aaggttcaat gttgtccatt tccaacggcc atactttcaa
      241 gtcaaactgg ttatcctgaa tttactttct tagactttac tgatgaaatg gtaaaatatt
      301 caaatgtttg gaaaaattta aaagtcaatt ttgatagtat atatagtggt tttttgggtt
      361 caaagcatca aattgagata gtggctaatt ttataaacga ttatcccaat gcttttatcg
      421 tagttgaccc tgtgatgggt gataatggag ttatgtatcc aatattcaca gaagaaatga
      481 gacaggaaat taaagaatta gttaaacatt cagatttaac tactcctaat ttaacagaag
      541 cttgtttctt aactggaaat gattatacca aaagcgatta taatagagat gagcttatat
      601 atattgcaaa atctgtatct gatttgggac ctagcaaagt cgttattact ggtatattag
      661 aagatgataa tatattaaat ttggcttaca atagagataa tgaccatgta ttctttacat
      721 cagtcaaata taataattgt tcttacagtg gaactggaga tatattcaca tctatacttt
      781 gtggtatgtt agtaaataaa catgatttgg gagtagcggt taatactgca acagatttta
      841 tatataaaac tataaattac acatctcaat ttgatactga tagaaatgat ggagttatgt
      901 ttgaaaactt tttatcagat ttaaccaata tataaaatca aaaaaactat ccattttata
      961 aatacctata aatcataaaa tttaggtatt tacaaaatgg atattattta ctaaactatt
     1021 ggtatttcaa ttgttaccat agcaccacca gttttcacag aattttcaat acataaagtc
     1081 ccattatgtt tttgtacaat attatttaca atatagagtc ccattccata atgagattta
     1141 gaatttctac tcaaatctcc tgtataaaac tgttctgtag cttttgacaa atctgcttta
     1201 gaaaaaccac ttccacaatc tgtaactaca aattttaagt atttattatg tactgataca
     1261 cttatgtata gtttactctg gcttggtgaa taatctatag catttgaaat tatattcata
     1321 atagctctga ataacagttc tctatctata gttatatttt taggcaaatt atcttgttta
     1381 taaaactcca catctaagtt tttagtacta gctaatgctt ctatttgcac taaaacatct
     1441 tttataaact cactcatact gattgcttct ttatttaata tatagccatt ctctgttttt
     1501 gatatatcta ttaaagtatt aatatatttt tctatttgag tgacaccctt tgagatatat
     1561 tccatgtatt ctaataactc aggattttca tttatttcaa ttaataagtc agtatttcca
     1621 tgtataattg ttagtggtgt ttttatatca tgtgccagtg cagatatctg ttcctttcta
     1681 ttttcttcta gcatccattg tttttctaaa gaatctttta acgccaactt cattttatcc
     1741 atagaaaaaa ggacattatt aatttcataa attttactag attctacaac aaaatctaaa
     1801 tcctgttgtt ctattttttc tgtagtattt tttaaaagct ccatttcaat attaaatttc
     1861 ttgccaaata cttttgataa tataacaatt tcaataaaaa atattatact aaaaataatc
     1921 atacccaatg tctctggttt tggtaaatat gctcttaaaa ttggagaact atactctgat
     1981 actattttgt attttattat aaaaacttca tctttcttaa aaaactttat ataactttct
     2041 gaatatgcat gcctttcttc aatatctctc attaaattcc atacttcttt tgattctttt
     2101 ttattaaagg tccctgaaat taaattccca tccaaagtat aaaccccata ctcatatata
     2161 cttggaatta tatcttcagt tactttttca cttgaaataa tctgttcttt atatttatta
     2221 aattgttttt ctgcataatt agctggtaaa attacacctg atgataataa tacagaaaat
     2281 gataacatta aaaggagtac aattgaaatt gtcataataa agaataaaga taaatatttt
     2341 ataaaaatag ttcttaactt ttcaactttt ttgttgttag attttttacc tccaagcatt
     2401 tgtaccctgt cctccaatag tttaattaat tcataattaa tacccaacca gttttttatt
     2461 tgattatttt atatcttttt attgctaagt tatattttaa cataataata taaataaaca
     2521 gtcaattttt actctattct tctgattttt taccttccca tttagaaaac caaatacata
     2581 aaattacaaa tgctaaaatt gttacaaaac cacaaattaa acctgttgat tgatatgctt
     2641 ttattgattc aacctttata tattcaaccg ttttagaact tgaaaaagac gaccaaatag
     2701 ttataaatct aacaccccat gcacaaggaa aatactgcca aataccagaa cccaatccag
     2761 ttaataataa tgctgaaagt aacatttcaa ctatacctac tccaattgat gttccttttc
     2821 cgaaccttaa acttaagaat aaatgtaata tgtattcaaa taaattacac ccaattaaaa
     2881 ttagaattga aatcatataa aagcttgatg taaagtaagc cttagatata ccaaatttaa
     2941 aacctaatac agacactaat gttgctacaa aaccaagaca tactaagatt atatatttac
     3001 ttataagagt aagatatttt ggattagatg ttgttaacat atgtttataa ttccctgcat
     3061 aatattcttg ttcaacaaac attgaacaca caacacttga caataaagga aaagctattg
     3121 ctataacttg taaatatgct actatcttta gtatattatc agctcctgaa accttataat
     3181 accataaaaa caatcccatc cccattaaag caatgcaaat gtgcattagt attataaaat
     3241 ttctctttaa ttttattaaa tcagaacaaa atattcttat taactgtctc atattacttc
     3301 acctctctat tattgaacca aacccttgta gctaaagcaa ataatacaaa taaaacaata
     3361 gatattaata ctcctggcaa aataacagca ttatttaaaa attcacttcc ctctggtaca
     3421 ggaagaccat ttggcagaat atgcaataca acagccatta atcttgaagg aatcgaatat
     3481 ggaaacatat accacatttc tgtagttgag aaaacagcaa ctccaacaat agtaaagaac
     3541 atatttgtta gaactgtaac aaacatacca aattttgaag ctaaaaacat acaaagagga
     3601 atctgccaca taaatagtaa aataattaat atacttgcaa tgatattttg acctattgaa
     3661 atagtttctt tatagattat cccaacaaaa actacaccta ttgcagaaat aattgatgct
     3721 ataaaacaac aaataataca gtaaatcatt tttccagtcc aaacgtcttt catttcaatt
     3781 ggcaatggta aaataccacg atatttactt ttttcatctt ttaatactat taaagaagat
     3841 attaatgtta atgctcctgg aagaaatgtc gtataccacc agttgaaagc agcattttgc
     3901 cctccaccta gtccacacaa aagtatacaa aacactggaa ctatccatat caacttcatt
     3961 ccaaaagttc ctttcatctt ttgaaattcc actctaataa atctttccat attactgccc
     4021 tctctttcta ttttcagcta caactttcat aaaaagttct tctaagtttt gttcttttga
     4081 tacttctcct tgataaccca ttactccatt agaaattatt cctatttcat ctacaacttg
     4141 ttcaacttca gacaagatat ggcttgataa aataactgta attccttgtt ttggaaaaga
     4201 acgaatcaat tctctaagtt cttcaatacc aatagggtca agaccatttg taggctcatc
     4261 taagattaaa agttttgggt gatttaataa tgcaatagca ataccaaggc gttgcttcat
     4321 gcctagagag aattgacctg aacgcttttt aaaggtatct cttaaatcta cagtctctaa
     4381 aacttcttca atgcgagagt caggaagacc aagtaaagta gtatggactt ttaaattttc
     4441 tcgtgctgtt aaattctcat atatagcagg agactcaata agagaaccaa tatttgataa
     4501 atcctttcta gtccatctat gtccctcaaa cattattttt cctgaagttg gatttaaaat
     4561 cccagttatc atttttaata aagtagattt cccagctcca tttggtccta ataatccata
     4621 tatagaatta gatttaattg ctagtgaaat gttatcaact gccatctgct ccttaaattt
     4681 tttagaaact ccttttacct gcaaaatata attactcata attttcatcc tttcttttgt
     4741 caaacttatt tacaagaatc attctaaatc ataaatataa ggattttata agaattaaaa
     4801 aacaaaaaaa gatgtgttaa aaacacatct ttttttatct taattctatt aattttccat
     4861 tgtagtatat ctctacttct tcaatccaaa tgctattatc ttcttcttgg atgacatttt
     4921 taataatatt atttatagta tatattatat tttgttcatt caaataatca tctttccaag
     4981 aaactctact ttttttatct ggttctatca agtttatttt aacaagatta tctttttcct
     5041 taacttctac ttccatgtct aaaccattaa aatactcatt tgataatgta tctaagatta
     5101 atcgcagttt atccttaagt gaactttctt catcaatctg taagtacttt actattttct
     5161 catcctttga aattatctct ataggtattt tagtagtttt tttagaatta atcattttac
     5221 tagttgtttc ttcttttgca tcttcatcct tctttacaac tgctgattct ttagaatcaa
     5281 ctagctcaac tgcactgatg ctataatcca tacatgccgt actcaataaa gtagtagcaa
     5341 ttattaaaaa acttactgct attttattca taagtatccc tctcctaatt ctctttcgat
     5401 acttattata ccatagtata gattatattt ttatactttt aaatcaattt ataaattatt
     5461 atagtaaatt atttacaatt ctaagctatt tgaatttatt tttatagtat attctttctc
     5521 taaattcgac agatttttat tcaaaagccc ttttatttcc ctcgtatttc tatacttgtt
     5581 gctatgttct tcattatgtt cataatcata tgaatcattg tcatattctt catcattatt
     5641 aaattcttca attgcttctt ttgactttat atcacaatta aatttatact tatttactgg
     5701 tatatcaagt cttaccttag aatttggaac ttctatgtcc aggaacttag caattggcat
     5761 atcagagtac aagtaaaatt cactttcata atttgaattt ctacctattt tctcttttat
     5821 atttacattt tcaggtatat actcttctat attattgata aatacatctc cattctctga
     5881 agatatatcc aatgactctg attctatgtt tacattttta acgcctaaaa ttgactttgt
     5941 tgatagatga agattgctat tagatattaa gtttaagttt tctaatctat ttactttatt
     6001 ttctgttata gctctgctaa aacctccaga agaagtttta tatgttatat tatttgaatc
     6061 tacatcatca gatataaata agtacgaact tatacttgat gcatttatat ctacattatt
     6121 tggtacatat attctaattt ctctataatc acgtgaaaac atactattta tagatgtatt
     6181 taataagtgg tcgaaacttc taatcttttt aattttattt tcagattttc tttcgccttt
     6241 tacaactaaa gtcttatctt gtagggttac actgtatttg taaaattcat tattccctct
     6301 tgttgtcaca cgcacatctt catttgaact ttttcttatc ataacattta tattgtcaac
     6361 agctatatct aacttgtcaa tatttacttt ttcttgataa agtttgtttt ctttgtttaa
     6421 ttcagcttcc ttttctaaac caaagtttat taggcttggc ataactaaaa tccctgacca
     6481 tgttgtccct attataccta ttaccaataa aactgcagca aatatagcaa gttttttatt
     6541 catgtttatc ttctcctttc ttattttcta tatcttcaag gtccttatat atcgcctcaa
     6601 aatttatact atcatcatta tattcatcag tttcttttat gctttcttct tctctattgt
     6661 agtaatcatt attatatgaa ttttcattaa tgtctttatt caaatcttca tcatttaaag
     6721 tagctttttt tcttttttcc atcttttcat ttgcctttat ataaatattt tttctcttta
     6781 tccaatgtat ataattattt agtaattgtt tacctagttt tatggcaaat aataaaattt
     6841 gccatgcaag tatctcaagc cctacaaatg ctatggttgc gaaaatacaa aacatcataa
     6901 catctggaac cattgctata aatttaatca caaatggaat taatataaca aaagcaatta
     6961 atgagcatac taatgcaata gtagcagata caatagcaca tacaaacgca aatgctggaa
     7021 caattagaaa taaagataac actactaata acccctttat aacttgtctg gataatcctc
     7081 ctacaccttc ttctatactt ggtgtaagtt tatcatttat attttctttt gatttattgt
     7141 acttttcctt agcttcatct ttcaagatag aataatcctt ttttacattt gatttaaatc
     7201 tactaaattt accatttaat atgtctgtat tcattatttt tccatctata ttttcttttg
     7261 atttactata cttttctttt gcttcatctt ttaagataga gtaacctttt ttcatatctg
     7321 atttaaattt actaaactta tcattcaaaa catctgtatt tattattttt tcctcttcaa
     7381 tatttttttc tttagtttgt aagataagtt cttttgctat agattttggt gagccaagtg
     7441 atgctattat ctccatatca ctctttccct ctataagacc atctacgaaa tactcttcat
     7501 aatctctcaa tatatcatta acttcgctct cagaaaaatc tttttttaga taatccttta
     7561 aaatttcaag aaactcagtt ttattcaagt tcattacctc cctcatcttc acttattaaa
     7621 atctcaacag cctttacaaa ttctctccac tcttccatca gattggataa attctctttt
     7681 cctagtgaag ttattctata gtattttcta gcaggacctt cattagattc tagtatatat
     7741 gtttcgaaat acaaatcttt tgtaagtctt cttagaatag gatatattgt tccttcattt
     7801 acatctatta ctttagagat gttatgtact atctcatatc catacatatc tttcttagat
     7861 atgagagcaa gtacacaaat ttctaaaaca ccctttctaa attgtgtgtt catatactcc
     7921 atcctttctt taaatacaca gtattgtgtt ttacaaggta ctatgttaaa acttcttata
     7981 tcctcataat aacacagtac tatgttctgc acaatagtta tttttaaata ttcttgttga
     8041 ttaaatcctt ctataaatgt ataatactct aatcagatat attttatgaa agtggtgata
     8101 aattggctaa aatgaaagat gataaaacaa tattaatcct aactgctcaa tttggtgctg
     8161 gacatataag tgctgcaaaa gcagtcaaag aatgtattat tgaaaaatat agtaactata
     8221 atgttgttat acaaaacttt ataaatgcaa gtattcccat gatgaataag cctatggtaa
     8281 aactttatga aaacaataca aaatatactc ctggattata taattactat tattacttta
     8341 agaaatcgtt tgattcaaga catgatttat ctcataaatt atatacacct aaactttctg
     8401 agtatattgc tgatataaat ccagatttga ttatctctac atttccacta gctgctgctt
     8461 gtgtaaataa ctttaagata aaaaatcctg atataaatat acctacactt acagttataa
     8521 cagatgttgt agatagtatg gaatgggttt ttgaaaatac agatttgtat tttgttcctt
     8581 ctcctgaaat aaaaaataga ttttttcaga aaggaataaa tccagactcc ataaaggtta
     8641 ctggagttcc tgttgataag agatttcaaa ttgaaagtaa agaactatgt tgtgataaat
     8701 atagactatt acttctaggt ggaggaagag gtctatttga tatagatgag gattttatgc
     8761 attggataga tgaatttatt gaagaacata gtaattctat agaaattaca atagttactg
     8821 gtaaaaacaa aaaattatat gacaatctaa ctcataaaaa acctctaaaa aatattaaag
     8881 tacttggatt tgtaaacgac atgtataatc taataagaga atgtgaccta atgctaacta
     8941 agcctggagg agcaactata tttgaagcta ttcaatcaca aactccagta ctcgtaaaaa
     9001 tgccaaaagt tggacaagaa attgaaaacg ctaaatttat aatagataag ggtcttggca
     9061 tgatttatag tgatgaccta gaccttaaaa acatatttta taaattagta tcaaatgaat
     9121 ttgattctat aataaacttt atgaaaaaaa atttaaaaga atttaaaaca gtcattcatc
     9181 cagagaaaat tgctgattat atatcagaat taatagataa tcattacagt taaattgtac
     9241 tattaaaaaa tttcaaacat aaaattaaag gcaatcaagt atgcttttac acttgagaat
     9301 ttactcatag tgtttaataa tacttgattg cctttatcat atcaaaatca aattgttata
     9361 ttagtatata tttttcttgt actgcatata cagttgttgt atttcttcca tacttctctt
     9421 taattctatt tgtaaatctg aagcagtctg gtaatcctta ttttctatag cttctctcac
     9481 tctagtgaat agtgattctc tagttaaagt atccttcata atattgaact ttaaatccga
     9541 tattgagttc caaactacct catcaagcgc atccatattt tcttttgtgt gtatcttgac
     9601 aatacttctt attttttgaa tcccagcctc tattattcta gtttttaatt tcttttgcca
     9661 cttatctata gctccaatct taaatgattc tattatacta tcagtaaata catcaccttg
     9721 ttttaaagat tttaattttg aagcatatat ttctaagttt ttcatatttt cataaactgt
     9781 agccggtggt attccaaatc ttgcatttct ttcttccaaa ctataatgtt caaatacatc
     9841 attttcatct ctatatgctc tatctttttc taaatagaaa ctttcttcac ctacattttt
     9901 agataactct ttttcaagtt cttttgtact aagaccactc ttagctgctg cttttatacc
     9961 atccaacatt gtttgataac atcctgctat tactaaatac gtgttagata gagggcttgg
    10021 tgaccttaac tcaaatctta cagtctttgg atttttcata tctcttatta atccaacaag
    10081 cactgaccta tttcttgatg gaatctcata actatgacct aaagatgtaa ctatgcatac
    10141 aggagcttca aatccaggaa ctaatctatt aaatccatca ttagaagctg ttacaattgg
    10201 gtttaatact tcataattgt aaagaagtcc cataagagcc ccatatccta gttcacttag
    10261 ataatcttct tttaaatctt ttggcgcaaa tagatttttt atacttccat cctttaactt
    10321 cgcacttact cctacatgtg tatgtccacc acttcctgct actccatgaa ttggttttgc
    10381 tttaaatgta acttctagtc catgtgatgt aaatacatcc tctataacat ctcttactaa
    10441 taattcattg tctgctgctt gtaacggtgt agaaaacttc caagaaacct ctaattgctc
    10501 catagcatgg tttgttcttc catctatact aatagaactt tgtattcctc cgacctcttt
    10561 gtgagccatt tcagggttta cacctagctt ttgaagtata attaaacttt tctctaaaca
    10621 agttcttata ataccatgag ttcttttcca gtattgctct tttaggcttt gtgatacata
    10681 taatttctct aagtcagctt tatcctcagg agtattaacc cagaactcaa gttctgtagc
    10741 tgctgttaac attatttcct ctatatcatc tactgaatct attcctatgt tatttattac
    10801 atgtggatat tctctaaata tttcatacat tgacttttta aagtacttat ctgctttttg
    10861 aagtactcct ctagaacata cttttttatt atcatgtatt aaaaatgcag gtatctttaa
    10921 tgttccaaca ggtagaccta cttcttcgtc tatatattcc atattgtaat ctacatacca
    10981 atgacaactt ttatctggca ttaaatctac tttcgcatta tttaatgtag ctatattata
    11041 aagctcaaca cttgaaccat cagtctgtat agctgattcc aaaaatttat ctatatcctc
    11101 caaaaataat tctacaggaa tcttttcatc tgttgcattt cctcctaaat caacccccat
    11161 taaggaaaca aatcttatat ttttattgct ttttaggatt tctcttaatt cttcattagt
    11221 gtgtttatct ttctctatta cgtataatag actatccatt tttccactct cccataattt
    11281 ttatgtatat tttattttgt taaatacaag ataaatttca ttttccttac atttatgaat
    11341 ttttcttgca aattattcgt gttttattcg tgtttttatt tatattatta tatatcaagt
    11401 ataaaaagtt cgcaatattt tttattggaa aaaatattat acatacaata gtttaacatt
    11461 tttacaaata tggggtatac taaaataaaa gtatttttat ttgaaaattt aattttattt
    11521 ataatataat ctatagtaaa agaaataata aaaaaattta ggagataaac tttatggaat
    11581 acataatttt cgatttggaa tttaaccaag gatttgataa aaaactcaat aaaactgtat
    11641 ccaatgaaaa gtgtcctttt gaaattatac aaataggagc aattaaattg gattctaaat
    11701 tcaatataat agatacgttt aatagctatg taaaacctac aatttataaa gacatacatc
    11761 cctttattag tcgtatgact aatattaaaa gttctgattt taatgattcg cctactttcc
    11821 ctgaggtcta caacaatttt ataaaattta tatcatcaca agaccctata ttatgtgttt
    11881 ggggagctgg agatttgaag gaattataca ggaatatcaa ctatcacaag ctaccatcaa
    11941 attccttacc taaatcttat attaatatac aacaacatgc atctaaatat ttcaataatc
    12001 cagcaggaaa aagcattggc ctacaaaatg ctatttctat attggaactt gatgaaaaaa
    12061 tgtcttatca caatgcttta aatgatgcat attatactgc taaagttttt attaagatat
    12121 acaatccaag tatagtccca gatatatatc tttatacgag tataaaacct aaaactataa
    12181 gatattctaa caaaaaaaga gttgattatg ataagttatt tgatgaattt aggaaaattt
    12241 taaatcgtga attaactaaa gacgagaaaa aaatcattaa cttagcttat aatatgtgga
    12301 aaacaaatca atttacatta gaaaatgtta agcaaagaaa aaacaaatag agtttattta
    12361 gataaaaaat aggcatgtag attaaaaaac tagttaactt taaatttata tctacagccc
    12421 aatttttatc ataattaatt tttattttat ctctaattca actggacaat ggtctgaacc
    12481 taatatttca gtgtgtatat ccgcacttac taatctatct tctaattttt tggatgcaca
    12541 aaaataatct attctccacc ctgcattatt ttttcttgca ttaaatctat acgaccacca
    12601 actataaaca ccttctttat caggattaaa atatctatat gtgtctataa acccactgtc
    12661 taataattta gaaaacttct ctctttcttc tttagtaaaa cctgcatttt tcatattatt
    12721 tttaggattt tttaaatcta tttctttatg tgcaacgttc atatctccac acacaattac
    12781 aggcttatga ttatctagtt tggttaaata atctataaag tcgtcttccc atctagttct
    12841 atattctagt ctctttaatt cagattgaga atttggcgta tatactgtaa caaagtaaaa
    12901 atcttcaaac tctagagtta tcactctacc ctctttgtca tgttcttcta tatttattcc
    12961 atacattact tttaaaggct ctttttttgt aaatatagca gtacctgaat aaccctttct
    13021 ctcagcataa ttccaatatt gaaaatatcc tggtaaatct aactctattt gcccttcttg
    13081 caatttagtt tcttgtaaac aaaatatatc tgcatctact tctttgaaaa agtctaaaaa
    13141 tcctttccca acacaagctc ttattccatt aacattccat gatataaatt tcatacttat
    13201 actcctcatc taaacattga cacactcccc atagctaaag atgggggctt ttcaacaatt
    13261 ttattgtaaa ttcttaattt acttttattt taacaatgct atactataat ttcatctaaa
    13321 attactattt acttaatacc taaaaatata tgttttaatc aatttttaac ttttttgtta
    13381 aatcttcagg gctcattgaa gaaacataca tatttgttcc aagttcaaat cctccaaaat
    13441 tatattttct tgggattatg tgcatatgaa cattaaaata cgattttgtt tcaatctttg
    13501 ttggatgagt gtgaatacat aaattaaatg gcatatatcc attgatattg tataacttct
    13561 taaacaactt ttcaaatatt gtagaaagct cctgtatatt attatcattt aactcaccaa
    13621 actctatttt atctttaaat attattctta cttctccact atacttggtt gcatatggta
    13681 taagtaccaa aaaagcttct ccattataaa caactcttct atctaaactt atttcatttt
    13741 ctattaaatc atcatacaaa gagcttttat tgctgtcata atactccttt gaaaccaaca
    13801 gttcattcgt aatttcaggt ggaataaaag acatagacaa tatttgagca tgtggatgca
    13861 tcaaagatgc tcctgccttt ctcaagaagt ttttgaagat actaacatac tctactttat
    13921 catctttcga caaatctcta aatctatttc tatacataaa aaaaacatct tcaaattctt
    13981 tttgactcat gttataaaaa ctacgattat gtttatcgct ctctatcatt acttcatgaa
    14041 caccatatac atctcttgcc atatcatcaa ttataggaaa tttattgtag acagatttta
    14101 caagccatcc ttcttcacta tctatttcaa ataatgcatc agttgcatga gcttcatttc
    14161 ctttgcaaaa tgggcattct tcatcatatt cattagtttc ttcatcttca tttgggatat
    14221 ctgctgtatc taaaggtctt tttaacctat ctgtagcaaa tataacaaca tcattggtaa
    14281 ttggatctat tcttaattct ttcataatta acacctctta gttaatagcc tttaatataa
    14341 ttatatgata tttatc
//
LOCUS       DAMJZE010000099        14261 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJZE010000099 DAMJZE010000000
VERSION     DAMJZE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.100"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(144..1574)
                     /gene="dcm"
                     /locus_tag="PC541_002469"
     CDS             complement(144..1574)
                     /gene="dcm"
                     /locus_tag="PC541_002469"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDF2839925.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            1787..2503
                     /locus_tag="PC541_002470"
     CDS             1787..2503
                     /locus_tag="PC541_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDF2839926.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(2748..3026)
                     /locus_tag="PC541_002471"
     CDS             complement(2748..3026)
                     /locus_tag="PC541_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839927.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(3274..4608)
                     /locus_tag="PC541_002472"
     CDS             complement(3274..4608)
                     /locus_tag="PC541_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2839928.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(4621..5364)
                     /locus_tag="PC541_002473"
     CDS             complement(4621..5364)
                     /locus_tag="PC541_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839929.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(5385..5699)
                     /locus_tag="PC541_002474"
     CDS             complement(5385..5699)
                     /locus_tag="PC541_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839930.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(5703..5897)
                     /locus_tag="PC541_002475"
     CDS             complement(5703..5897)
                     /locus_tag="PC541_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2839931.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            6181..6897
                     /locus_tag="PC541_002476"
     CDS             6181..6897
                     /locus_tag="PC541_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2839932.1"
                     /translation="MIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFD
                     NYSAKLIGNNISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALAT
                     YTKIEDLFGFPLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDK
                     SEFDLVIHILKNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGL
                     VENFIQENSNGLIEDEDEEY"
     gene            6994..7746
                     /locus_tag="PC541_002477"
     CDS             6994..7746
                     /locus_tag="PC541_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2839933.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            7736..8902
                     /locus_tag="PC541_002478"
     CDS             7736..8902
                     /locus_tag="PC541_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2839934.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(9019..9249)
                     /locus_tag="PC541_002479"
     CDS             complement(9019..9249)
                     /locus_tag="PC541_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839935.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(9498..14069)
                     /locus_tag="PC541_002480"
     CDS             complement(9498..14069)
                     /locus_tag="PC541_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2839936.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
BASE COUNT         4838 a         2339 c         1479 g         5605 t
ORIGIN      
        1 ataaataagt acataataaa attaaagaga gcaatttttt ttgctctctt tcaaaattta
       61 tattaaattg taataataaa aataaaaacg ctagttatct aaggctaata ttttcaacga
      121 ttatataaat atctacataa cagttaaatt acatactctt cttcagttat tgcaacttcc
      181 tcagtgacat atattttttc tattttatta agataatcta ctatacttaa tccaatagcc
      241 tttcctagct taacaggaac tgcatttcct atctgcttgt acacctgact aactgaccct
      301 gtaaactccc actcatcagg aaaactttga attctagcat actctctagt tgtgaatggt
      361 ctagtctcat ctggatgaca cctttcagtt tgcttttgtg caggactaca tgttaaagtt
      421 aggcatggtt catcccaact tattcgcctt gccatacctc tttttccgcc accgctataa
      481 taactttttc ccatataatc tcttgccacc tcatctggca aatctatcca acacccacct
      541 ggtggtacca tatctaacac ttttttcttc ctctcaggat atctagctcc ttccgatttt
      601 ggaacatttt taagtgcatc tcttaacgta gcaacatatt caaacgactt agggaactta
      661 aaatccacat gtgacaaatc ttttcttgtt cctattataa taactctttc cctcttttgt
      721 gcaactccat agttcacagc ctttagtatc tcatatttta cattgtatcc aatctcgcta
      781 aatacatcta tcatagtctt taatgttttt ccaccatcat gagatacaag acctctaaca
      841 ttctctgcta aaaacacctt aggctttaac tcttctagaa ttttagcata tgaataaaac
      901 aatgtccctc ttacatcctc taaccctagc ttcttacccg catagctaaa tgcttggcat
      961 ggataaccac ctgatagcaa atctatctct atattattat caatataatt ctttattcca
     1021 ttttccgcta ttttaacaat gtcaccctgt ataacattcc agtctggtct atttaatttt
     1081 aatgtatcac aagcatattt atcccattct actaatccaa cctctttaaa accagctttt
     1141 tcaaggccta atgctaatcc tcctgctcct gcaaataatt caacacatgt atatgctctt
     1201 ttagctagta cattacttat atctatatgt ttattatttt tatttctctc tctaggattt
     1261 aattcatatt cactgagttc tttatatgta cagtctctat gctctatata atctttatta
     1321 ctgctacaaa tatttgtaaa tttcagttgc ttttcattta catcttccaa acacataatt
     1381 tcatcgaatc caatatctaa cacttcacat aatttttcta cattactttt tataggtgta
     1441 aatttttctg ataatattgc agataactga ttcttagtta tgcccatatt taatgccagt
     1501 tgattctgac ttcttatatt tttctctttc ataatttttc tcactctatt tttatcaata
     1561 gttatttttt tcatttagtt cacctcttaa taaataatat acccttaaat agttaataaa
     1621 atacattaac tatttaagga tatattatca taattctaat ttttatgtaa gtagttataa
     1681 aaacttaaaa taaaatatct gtaaacaacc taatattacc tcataatttt acacattatg
     1741 gaatatattg tataattaca ttaatacttt ttagggggat tattttatga ctgaattaga
     1801 aaaacaagaa atactagata aagccaaaga tttttttaca aatattttgg ttgctaatca
     1861 taaaaagaat actattaagt taaatttaaa ggcatttaat ataaatcctt ttttagttaa
     1921 atacttagct agtttcttaa caggatcaaa taaacctgaa gatattgcta aagcattgat
     1981 ttatccaaga gttctaggca cttcgattaa tacgtccttc ggaactaatt tacaaaaatt
     2041 ctgtattacc actttagatg gttttgcctc tacaacttct ggcattgata tagagtttat
     2101 tgattgtatt gatggtagaa ggaaattctg tcaaattaaa gctggaccaa acactattaa
     2161 taaagacgat gttaaaacta taattgacca ctttagctca gttaaaagac ttgctagaac
     2221 aaatggactt tcattatctt ataatgactt aattgttggt gttttttatg gaacagaaaa
     2281 tgagcttaat gctcactata aaagaattaa tgaagagtat ccagtagttg ttggtcaaga
     2341 tttttggtat aggcttactg gtgatgaaaa tttttatttt gatttaatag atacaattgc
     2401 tcaatctgct ttagaagagg actgtactgt tttacttaaa aaaacaatat ccaatctagc
     2461 tgatgaaatt tctaaagata aatctttatt caatcaaaag taatataaaa atgattaaat
     2521 agcataattt acatttttag atatttaaaa ataatattca ccaaacaaaa ttattaagta
     2581 aaatgaaatg tatcttataa aagctgagct ataagtaaat ctttgtaaaa acaaatttac
     2641 ttatagcttt tttatgttca aaaaacacaa attatatact atttaatata ttataaactt
     2701 ttatttgtta atactctcta ctaaatttct aacatctctt atataaatta actatttaat
     2761 aattcaatca taaatctagc tcctgcctta aaaccaagct tataaccaag ctctctttca
     2821 atctctataa ctgaattaaa taaatcattt aactctatca atacatccat tttcttttct
     2881 tctgataaat ttttttctag taatttatta actttcttaa tttctttaca tctatcatta
     2941 taatactcat ctttttctag tactttccta tacatgtgtt catttatcca ataatcaaaa
     3001 tatatttcta taatttcatc atacatgtta gtacctcctt gactttcatc tggtacttaa
     3061 tatatactta acttgtgtag attagtgtac attaagtacc taatttataa aggtagttgt
     3121 tatatttata tggcaactac ctttttctaa taaatttttc aatctaaata ctatatattt
     3181 taaatcttca ttgcttacta ttgaggatta tatacttttc ttctaacact ccttaaacaa
     3241 tagtttatat atcacttaat aaattatatt ttttcatacc tcctttacct tcttacattc
     3301 cttaaaagaa aacaaacttc ctatatacca ataaggtcta aagcctgatt ccccatctct
     3361 atttttagca actataattt cacttaaatt agctccaaaa cctttttctc tacgtcttaa
     3421 ttcttttata tcctctattc ccattgtctt acaataatca agagcctctt tctctgtagg
     3481 tctatgaata tatatgactg aattagaatc catatatatc tgttttgaac ttctcattac
     3541 tctatctcct tttggccttt catccttcaa ttcatcattt aattgagcca actgtataat
     3601 tggaatatta aagtctaaag taatcttttt tatttgcctt gataaatcag caaacttagc
     3661 ttctccaact tctttacctt cgtaatccaa aatctgaaga taatcaatta taacaacatc
     3721 tggtttaata ctttttattt ctctcttaat tcctgatatt gtattgactg aatcattaat
     3781 atagagatta ttttcctgat ttaaagtact taaagcattt atgtatttaa ttatttcttc
     3841 ttcatttaga tttctattct tcatttttac aactgatatt ccagttaaag aagctatgtt
     3901 tctaaacaaa atttgtatat cactcatttc cccacttata aaaagtactt ttttaccttg
     3961 aaataccaca tttctcatta tctgtaaggc tagtgctgtt tttcctcttc cacttttggc
     4021 tgctattgta attaattcac cactatatag acctccaata gtttcatcta aaaattttat
     4081 tcctatatcg ataccttctt caatttcatc atttatgtat tcaaaggctc tttctgatat
     4141 agctgttata ctagaatcct tcttaaccaa tttctgatta acttcattaa cgtatttttc
     4201 taattgatat ataagtgtat tatcatctat ttcacctaat ttgaatttat taaatttatc
     4261 aattatattt ctatttttat atttttcaat aactatctca atataatggg atacattacc
     4321 tatagttgga actattgttg atagttttgt caaataactt atacctccaa cataatctat
     4381 aagccccttt ttctttaaat cttctgtaag agttattata tctataggct catccctttt
     4441 atgtatatct ttcactgaat tgaatatatg tttgtgctct tgatgataaa acatatcttc
     4501 acttattaat atctcaatct cttctatttt cctagaatct agtaaaatac tacctaatat
     4561 agattgttct gcttctgtgt tcttaactaa attactcata ttatccataa tataaactcc
     4621 ctaccacttt aattgtgaat catctggact atatgcttct gcatctactt tgacattaat
     4681 tgatttatct tgataattgc cttctaatat cttaataaaa ttatttgggc tcattatcca
     4741 atcaaaattt gccttccaac ctttttcatt gcttccattt aaaaagctag ttttactaac
     4801 tatatgaata atttctaata cagtctcttt atcatattct tttattctag cattaattac
     4861 tttttttcta gcttctgtaa gtttctttac ttttgatagt tctgtacaag taatattgaa
     4921 tttttctata atttcactgt atattttact tttttcatta tttacattct tgttagttgt
     4981 tagttgcttg ttaattgcat gttgattagt ctgttgattt tcttcattag attgatatag
     5041 ctcccagttt tcaatagtta taagcctatt tttgtttgtt gatttatttg ttagaaatcc
     5101 atatctttca aatttcttta aagcagttct tacattctga atacttacat cttttgaaca
     5161 catatttgct atactttcca aagatgttat taactgtcct gatttaactt catattcttt
     5221 acctccataa acccatttat taggcttatg attaactgac agtaataggt ttatgagtat
     5281 atctctttgt ttggaattac aatctttata tagtgggctt tcttttatct ttctatgaag
     5341 ctttatccaa cctagattac tcaaatacta tcacccactt aagtctataa ttttatacca
     5401 tttgcatttt caataaacca attatctagt ttatttttta ttatatacac tctattatct
     5461 atccatatac aaggaaatcc tttattttta gaatgacata actgtctaaa ttttgattcc
     5521 cctatgggat aaattttaga agcttcttta caagtgataa gttgagcttt atctcttcta
     5581 tatgtctgta tctccttgta ccctttttcc ttttcttgtg cttcttgttc ttgcttgaca
     5641 agtatattca atgtagcttg tagtgtttta agaacttcta gattatttgt atctgccata
     5701 catcattcct ccttatcaaa aaataattct tctacacttg tatctagttc ttgagccaat
     5761 tttaacatca acatattact tggatttttt gatttacctc tttctaaaga agataaataa
     5821 tatcttgaga tgccaacttt ttcagctaat tcatattgct ttataccttt ttcaattctt
     5881 tttaatttga gtttcattga tttcctcctt tctttcatac ccattttgtg tatcctcatt
     5941 tctataaaat attctaacac ccatttggtg tattgtcaat atatttaagt gtatcaatag
     6001 acccatttag tgttataatg tatttgaggt gaaattatga tagataatac aattggaaaa
     6061 aagattaaac aatatagaaa atctataaat atgtcaaggg atgagttagc taataagatt
     6121 ggttgctcag tttatgctgt agctaaatat gagcaaggcc aaagaaagcc tagtttagat
     6181 atgataaaaa aaatatctac tgcattatca attcctataa ataggcttat tattgatgaa
     6241 agtgatttag atacttcaac aaataattat actaatttaa aagaattgga tttagttgta
     6301 aaaacatttg ataattactc agcaaaactc atcggaaata atataagcaa atttagaaat
     6361 ataaataata tatctctcaa agaactctca gaaaaaataa attgttctga gaatgaaatt
     6421 ataaattatg agaatggaac aaaaatgcct agaattgaaa tattaaaaaa aatagcactt
     6481 gctacttata ctaagattga agacttattt ggatttccat tagaagagat aaaaatagat
     6541 aaccaaacaa tcctctatag aaatccccag actggatata ttatagataa ctttcttatc
     6601 gattttataa ataaagatat taacatagat aatactccag tttcttatta tatagataag
     6661 tcagaatttg atttagtaat tcatatatta aaaaattatg gttatgatgt aaaaacagat
     6721 tataagacaa agtttgaaat tgcagataat gcagatacaa taaaaccaat tagcttaagc
     6781 ttctctgact tcaaagactt ctccaaaaat ctaaattggg tgataaatgg tcttgttgaa
     6841 aattttatac aagaaaactc taatggtctt attgaagatg aggatgaaga atattagtat
     6901 tgcacatttt caaaatatga gtttacctat taacttttat acattcatag ttaataaata
     6961 aaaattagat atgatatgaa aggagctaaa atcatgaata aatcatatat accaaaatac
     7021 acacataaat tccctactaa tctacctcat ggagataaaa aatatacttt aacatacatt
     7081 agaagaatga taagtgaatt tgtatatgat atgggtaacc tagaaaataa cccctttaca
     7141 ttcccagaag tacaaacatt acttgatggt ataaccgtag gtggacataa attaagtgac
     7201 caaaatcaaa tactaaatat aaaatcatca tgggattatg taataaaatt aagtaacaaa
     7261 gaaaacttat ctttaaataa agatacaatt tgctctatac ataaaatagt tgcaaaagat
     7321 gaagctctaa tagttggaga ttttagaaat ggtaacatag gtatagctgg tactactcaa
     7381 tatgaatgca tagaagccac tctattaaat gatttatttg taagcgacat aaatatcata
     7441 aataaaataa ctaacccctt agaaaaggct ataataataa atttatggtt gtcctattgc
     7501 caattcttct atgatggcaa caaaagaact gctagactta cctcaaacct aatacttata
     7561 agtaatgata taggagttct atcaatacca gctaaacata aacaagaata caacacatta
     7621 atgctaaact tttatgaaac actagaagct gatgaagtta taaaattctt attagagaaa
     7681 tgtattacat tctttgatga atataactat aaatcatata aggagctgtt taaaaatggc
     7741 aactaaaaga gctaatggag aaggctctat cggaaaatat aaagatggtt ggcgttcccg
     7801 tataatgatt ggctataatg aagatggtaa accaataaga aaagaatttt atggtaagac
     7861 tcaaaaggaa gttaaagata aacttgatgc ttataaaaaa caatattatt taagctctga
     7921 tatttcagat gataaaataa ctttagagca ttggttttac aattggttat ttgaatatag
     7981 aataaaagat ttaaaaccta aatcatttga aagatatgaa agcatctatc gtaattatat
     8041 taaagatact gatttaggca atgttaaatt aaaggactta agagtatctc acatacaaaa
     8101 atattacaat agattgcttg attccaataa aagtatacct actataaggc aaataaacac
     8161 taaactgaaa acctgcttat cagaagctga aaaacaaggt cttatacaac gtaattattg
     8221 tactatggta aatataccag ttgagaaaaa ggagaataag cttgaaatat tgactcttga
     8281 acaacaaaag agttttataa aagctataga tggacataaa ttagaggtat tatttttact
     8341 tgctcttggt actggactta gattaggaga attattagga cttaaatggt ttgatattga
     8401 ttttaaaaag tctaatttaa cagttaaaag aacccttcaa agaacatatt ttatagataa
     8461 aaccggaaat agagagctta aagtattaga gcaagagcca aagacatcaa attcttacag
     8521 aactgtacct attccaaaag atgttttaaa taaattgaaa gagcataaag taaatcaaaa
     8581 ttcggatata ttaaaggctg gagaattata tagaaatgat aattatgtat tttgcaatga
     8641 attaggtgcc cctattgatg ataaaagacc tttaaggaat ctaaaatcta tattaaacag
     8701 cttagatata gagcctataa aatttcatgg tcttaggaaa acttatgcga ctagactttt
     8761 tgagaatgat gtccctccta aaactgtaca agttttaatg ggtcattatg atatatcaat
     8821 aactttaaat atatatactc aagttatgga agataagaaa attgaagctg tagaaaagtt
     8881 ggataaaata ttttcattgt aaaaatcaaa tatataaaaa tactctgatt tcatatagtt
     8941 aattacaaaa ccaaaataag gatacaaatt aattttatat tatcttgtat ccttattctt
     9001 gtctagtttt ttaaatattt atcttggata gcctttatga tacattaatc cattttctaa
     9061 cattagttct tgggctaaat atatatgacc cttacagtgc atatctgata taccctcact
     9121 tataagttta tatgtttcag aagtatataa ataatccata ctttcttcca agctcttacc
     9181 tgtttgttta gaaaattcta atatgatttt tttatatagc gattgtaaca atgtcttatt
     9241 agcttccata aaagctatac ctcctctttc aaatatttgt ttatcctatt ttgactaact
     9301 atatatttat tagttattta attagccata ataattcggc tataagatat ttataatcta
     9361 atctcaatta tataaatctt tttgccaata tttaataatc aaaatattgc acttaattaa
     9421 ctatcttata agtattgata ataaaatctg tcttcttaaa tatacttaaa taaaacttct
     9481 ataattatat caaagtatta atattcactc tctattgtct ctcctacaag tctattttct
     9541 ccatttaggg tatatactcc tgatatagtt ggttgtatgt attttctatc catatatggt
     9601 tcttctaaaa ctcttttttc tgctagcatc gtccaaaata cactatatcc ttgttcatta
     9661 agatatttta agaaaatttc cttattaata aagaatccta tctcttcatt aagtaattca
     9721 ctactttcaa aacaaacaac ttcaccataa tcattatata gaatcgaatc tccctgacca
     9781 tatctcaatt tcaacccata aaatagagta ctacaaggtt tatgccatgt gccagaaaag
     9841 tcttcttgaa atatgtcacc ttctcgttca gtagtataac tataagttgg caataatact
     9901 ttataatcct tttctaagat attcttatgt tcatctattt gtacccactc ttctccacaa
     9961 taatatgaat tttgaaaaaa gtaatatcca tctgcccaat aatattcctt attataaaga
    10021 gtataattac tattaggttc agtaagccat ctacccataa agtcttttcc ctttagagca
    10081 ttcactattg gttctagttg gtcattttta attatatagc tattgattaa tacccacatg
    10141 tctttttcag gatttttata gttcttattt ttaaaatcat tttcctcctt ccaagtatac
    10201 catccattaa gtaataaaaa gtcttgattt ttgtaaacac tatttaccat atcatttatg
    10261 cttggtaaat cactaaaatc tcctaaccac tcattattag gtattggaga aaattgaaat
    10321 aattgattat gtactctttt attgttatta ttatttatta ctgtcatgac agttggatca
    10381 atattcctaa tatctggctc aaatgtaccc ttacaataaa tttctttttt agtaccataa
    10441 gcatctgtat acacttctat cttataatta tcagcagctt gcgctgcaag ttcatataat
    10501 gcaatccatt gatatttctt tcctattctt tcatatttac tatctcttaa tctaccattt
    10561 tgtactgttc tatcaaattt accatgttta tttacatcat atcccatatc aaaaactttc
    10621 ttaatagcta tattctttaa atcgttatag tctagacctt tccatttatg gaaatagtgt
    10681 tggaacatat atcttccaaa atctccatac catcctggtt gtccatctct agtatattct
    10741 acttccatag aatttaagat agtgttttga ctccaataat aatctttaaa atctgatgcc
    10801 ttatagtcat acttatatcc ctcaatttct tcatcagttg gtactttagg aaaaattgat
    10861 ttatatggtg gttcaacatt catatctttt aatactgaac tatcaatttt atattttgca
    10921 taatctacaa tattttttgc ataatctctt aataaaatat tgggataaac ataatctaca
    10981 tcaaatattt tattataaac ataaactgcc aatgcctcta tcatctcttc tgattgctct
    11041 gatacaacac atccaaatgc aactgcatat aatcgttcaa taatatatgg gtcatctaca
    11101 tcttcaaatg ttttaagtaa atctattaca atatccattt tccctgttaa tagatttaca
    11161 agagcttttg tacttttatc tcttaagtct ctatttgaag aaattaaaaa cgttgcaatc
    11221 atcatagctg acaatctaat tgtttcattt gaagtattat caaaaccttc aaaatcaaag
    11281 caccatccta ataatcgctt aatagaagac tcttcttctt cataaacttc attaaatata
    11341 ggtataaaga tagcatctct atctgccata gtattttgta aaatatattt tactatataa
    11401 tctgcattta atgtatgatt aacctttgta gaaattaaga taagtacatt ataaaaatct
    11461 attttataat aagatttacc taaaactatt ttattaatat aatctagtgt tttttcagta
    11521 attgtagatt tcttacgcca ttttagtgca ttgataaatg cactaataac attataattt
    11581 gtttctttat ttttaaaaac ttcaaaaatt tcattaggta tatctgagcc tgtctcttca
    11641 gataaaacaa ttgccaatac ttctaatata tcctcatatt ctactaaata tttatactta
    11701 tgtgaaaaat ctactacccc aatttctttt aactctttaa ccaacaaatt tgcatataaa
    11761 taatcttcta acttctcata tgtcacatgc atatattctt catcatttaa attgactgtt
    11821 ttattaacta ttccatttgc tatgagttca tctattatca cattgttaat cttatactta
    11881 cttgcaatat ttattataat ctctacactc ttgtttgcgg gtattaaatt attcccaaga
    11941 ccatctccat atttatattt aatcatttca ttaagtactt ctgtaactac attggcatgt
    12001 ctactataat tgcatgattc agatattcta agattaagta tattcaaata atttttataa
    12061 atatctgtaa aactaatagt atctaagtta atctcttctc ctttaaaagt ttcacacata
    12121 attctcaaaa aaagagggtt tctaaattct tgatttggca ttggaatatt agaataaggt
    12181 attccataat aatcaaaata ctttttcata gctttatgtt ctatactaga aaatccttga
    12241 tgctctataa aaaccaaatt ctcttttatc ttgttattat tatctattaa agatggtata
    12301 tattcacttc ttattgataa tattagtcca atccatggat atttctttat ctgttctaca
    12361 ataccattta aataatattt ccaaagttct tttccttgac cttcattcaa tgcatctata
    12421 aataatatca cacgctgttg ctctttttct cctatttcat taagtatttc taataattct
    12481 tcaaactcac actcaatttc taatatttct aacatatgtt taattggtgt tttatccaac
    12541 ttaaaatgtt gccctaaaaa taatatagac ttatgcccat ccatatttct tttctcaata
    12601 gtatgtgcaa ttatatgaga tttccccata ccacctgcac tatgtaataa aatataaggg
    12661 ctattaacta cttttatttc cttagaatcc aaatatttta tatatacata gatatctttt
    12721 tgagtctgct tcaatttgta tatttcatct tctctttttt tacttttttc acagtcttca
    12781 tttataatta tatttttaaa attatttatt ctatcatcaa gtgctgcact tattttttca
    12841 aagtgctttt ttaacaactt gatatctaat ctttcattag ctttgtcata aaattgttgt
    12901 attatttctt ctataactag tacatctact tccacttcat ttattttctg tttctttata
    12961 cttgatagaa ctttatctgt tttttcatca aaacgttgtt taaattcttc attttttcct
    13021 aatgcattaa acgtattttc taaactcaat tctacattca attctggtaa atatctatct
    13081 ccaaggtttt ttatctgtat attattctga ttttgaaacc atttctctgt taatctacaa
    13141 gataaattag ttaacctact ttcctttaaa gacctatcct ttaatcttct atattcatct
    13201 ggcaaaacta tttccaaaat tcgttcaagt attattctta cacactcatg cttaccttca
    13261 ggatacataa tggtttgtat ataatactca ttttctaact ctattcgtct atgtttcttt
    13321 ttctcactac taactggaat aatagcataa tgacccgttc ctgcctctga tacttgctgt
    13381 aataattcca acggtctatc cttttccaaa ctacatccaa gaaataacaa ctctttactt
    13441 gtatatactt gtgtaagcat ctttctcaat tcatcattac tatatgcttt ttcatattgt
    13501 tcttttgtta acacaataga tgttgtaggc tctgtaatat ccccatgaat tttatacaat
    13561 actaattcat tatttctaag tgcattattt aatgcttcaa agtgacctgg atgtgacact
    13621 aaaactgttt tattgtgtaa tccataattt ttttctagta ctttatcata attagtagtt
    13681 ataattaatc ctgagccaaa aattatagga attaaataga cacatttatc aataaaatcg
    13741 tctttatctt ttgcatccag ataacctgcc ccaaaaactt tttttaaatg tttagaaaat
    13801 ctaactttcg ccaattcttc atataaaaat tctgcttttt tctcataatc ctctattttt
    13861 tcaaataaaa ttttatcctc atcttctaaa tattcttcac ttgtgttatt taaaaatgta
    13921 ctccatgaag gatatatgtc tttaaataac atagacatgc ctgctccaat atatgggact
    13981 acataaccat ctttaattgc atttaccagt tcatcaaaat ttttttgatt gtaaggctta
    14041 ttaaattcca taatttcttc aaatgtcatt attccctcct aaactatctc ttaataattt
    14101 caaccataat tcctatttta taattttatt atatcattcc attccatttt tttactacaa
    14161 ttctatgtta tagaatgaat tcacttactt acatttgtta ataatataaa actttctttt
    14221 aagataatta aaatttatac ttttaaatta tggataaaaa t
//
LOCUS       DAMJZE010000100        14055 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJZE010000100 DAMJZE010000000
VERSION     DAMJZE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.101"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            425..1318
                     /locus_tag="PC541_002481"
     CDS             425..1318
                     /locus_tag="PC541_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2839937.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1318..2712
                     /gene="gltA"
                     /locus_tag="PC541_002482"
     CDS             1318..2712
                     /gene="gltA"
                     /locus_tag="PC541_002482"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2839938.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3078..5297
                     /locus_tag="PC541_002483"
     CDS             3078..5297
                     /locus_tag="PC541_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2839939.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5444..6190
                     /locus_tag="PC541_002484"
     CDS             5444..6190
                     /locus_tag="PC541_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839940.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6190..8070
                     /locus_tag="PC541_002485"
     CDS             6190..8070
                     /locus_tag="PC541_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839941.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8189..9580
                     /locus_tag="PC541_002486"
     CDS             8189..9580
                     /locus_tag="PC541_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2839942.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPETIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9687..10727
                     /locus_tag="PC541_002487"
     CDS             9687..10727
                     /locus_tag="PC541_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2839943.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11216..11869
                     /locus_tag="PC541_002488"
     CDS             11216..11869
                     /locus_tag="PC541_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2839944.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            11945..12154
                     /locus_tag="PC541_002489"
     CDS             11945..12154
                     /locus_tag="PC541_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839945.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12294..12860)
                     /locus_tag="PC541_002490"
     CDS             complement(12294..12860)
                     /locus_tag="PC541_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2839946.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13021..13674
                     /locus_tag="PC541_002491"
     CDS             13021..13674
                     /locus_tag="PC541_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2839947.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13832..14008)
                     /locus_tag="PC541_002492"
     CDS             complement(13832..14008)
                     /locus_tag="PC541_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839948.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5499 a         1390 c         2377 g         4789 t
ORIGIN      
        1 aaagctggta ttgcaataga ttaaagcctt atacacgttt agtttttaaa gaaattagtg
       61 ttattatgaa aacctaagtt aatttattaa ataaataatt gaataaaaaa attttattat
      121 gtatttataa aatttttgtt acaaatttgg cataatatag tatactttaa gtgtgtatta
      181 ggttaaatct ataacaaatt agacgtatga tatagactta gtgtttattg gcatttacaa
      241 gaaatttgtt aatatcaact atttgtagtt tgggcagtta taattattat aggggggatg
      301 tccaatacaa aacaatcaac caagaactaa tattatatta taaagagtta tagttttgtg
      361 tgtttatgtt tttaattaaa tttaaagtgg gaaaaatata tttgttagaa tggagaatag
      421 cgagatgagt aataaaatag ttagtaagag acaattaaca gatagtattt atttaatgga
      481 aatagaggca cctagagttg caaaatcttc tcaaccaggt caatttataa taattaaaaa
      541 tgatgaaaaa ggagagagga ttcctcttac aatagctgac tatgatagag aaaaaggaac
      601 agtaactatt gtattccaaa cagttggtgc ttcaactaaa aagttagcaa tgtttgaaga
      661 aaacgatttt gtaatggatt ttgtagggcc attaggacaa gcaagtgaat ttatacatga
      721 agatatagaa gaattaagaa ataaaaagat attatttgta gctggtggag ttggttctgc
      781 tccagtttat ccacaagtta aatggtttaa agagcatgga ttagatgtag atgtaataat
      841 tggtgcaaga actaaagaat taattatatt agaagatgat atgaaaaaag tagctaaaaa
      901 tgtatatgta tcaacagatg atggtacata tggatttaat ggaagagtta cagacctatt
      961 aaaagattta gttgataatc aaggtaaaaa atacgaccaa gcaatagtaa ttggaccaat
     1021 gataatgatg aaatttatgt gccaattaac taaagaacta aatataccta ctatagtaag
     1081 tttaaataca ataatgatag atggaacagg tatgtgtggt ggttgtagag ttagtgttgg
     1141 aaatgaaact aagtttgcat gtgtagatgg tcctgaattt gatggtcatt tagttgattt
     1201 tgatcaagca atgagaagac agtctatgta caagactcaa gagggaagag ctatgctaaa
     1261 acttgaagaa ggcgatagtc atcatcatag taattgtgga tgtggaggta acaaataatg
     1321 gatgctaaaa aagtaaaagt accagttaga gaacaagaac ctgctgtgag agcaacaaat
     1381 tttgatgaag tatgcttagg gtataataaa gaagaagcta tggcagaagc taatagatgt
     1441 ttagcttgta aaaaaccaaa atgtgtgggt ggatgtccag taggaatcga cataccaggg
     1501 tttataacaa aaattaaaga agatgatata gaaggtgctg ctaaagtaat agctaagagc
     1561 agttcattac cagctgtttg tggtagagta tgcccacaag aaagtcaatg tgaaggtgta
     1621 tgtatacttg gaataaaatc agatgctgta tcaataggta aattagaaag atttgtagca
     1681 gattggtcaa aagaaaatga cataaattta tctgatacag agcctaagaa aaatcaaaaa
     1741 gtagcagtaa taggaagtgg tccagcgggt ctagcttgtg ctggtgattt agctaaaaaa
     1801 ggctatgatg taacaatctt tgaagcaatg catgagccag gtggagtttt aacttacgga
     1861 atacctgagt ttagacttcc aaaacaagct gttgttcaac ctgaaataga caatataaga
     1921 aaattaggtg taaaaataga aactaatgta attgttggta aaacaattac agtagatgaa
     1981 cttatagaag atgaaggatt tgaagctata ttcataggtt caggagctgg tcttccaatg
     2041 tttatgaata taccagggga aaatgcaaat ggagtatttt ctgcaaatga gtttttaact
     2101 agagtaaact taatgaaggc ttatagagat gattatgata cacctattag ttctggtaaa
     2161 aaagttgctg tagtaggtgg tggaaatgtt gctatggacg ctgctagaac tgcattaaga
     2221 ttaggttcag aatcatatat agtttataga agaagtgaaa aagagcttcc agcaagagct
     2281 gaagaagttc atcatgcaaa agaagaaggc attatattta atacattaac aaatcctaag
     2341 gaaattttag tagatgaaaa tggatatgtt aaaggtatgg tttgtataag aatggaatta
     2401 ggagagccag atgattctgg aagaagaaga cctatagaaa tagaaggttc tgaatttgta
     2461 cttgatgtag acacagttat aatgtcactt ggaacaagcc caaatccact aatatcttct
     2521 acaacaaaga gtcttgatat aaataagaag agatgcttaa taacagatga aaatggtcag
     2581 acttctaaag agggtgtatt tgcaggtgga gatgctgtta ctggagcagc aacagtaata
     2641 tctgcaatgg gtgctggtaa aacagcagca gcatcaatag atgaatattt aaaagcaaaa
     2701 gttaatgctt aatttgtaaa ttaaagagag ggtgtctcaa aatgaacttt ttagttcatg
     2761 aggcactctt tttgtacaaa atcaaatatg ttttaatcat ttcaacaatg aaaatttcat
     2821 tcctgtagaa agtctaactt ttaggtctta gtacaatctc tattttgatg tagccacttt
     2881 tttagatggt ttaataattt atcagttatt attgtaatat ttgatactaa gtaacataac
     2941 tttgtctaac tatgataaaa tagttataca ataaaggtct tgtaaggagt gaataatgtg
     3001 tggctaataa aatcagattt gtcaaatcta acattaaata atagaaaaat aaattgtgta
     3061 attaatggta ttttaatatg aaaaaaagag aaaaagtatt tgatgggcta gtttttaagc
     3121 atatattaat cttattaata ctattagttt tgattgattt aacaagtacc atcatatatg
     3181 caagtaactc tatggaaatt acttctagaa atatgattga ggcatcaaaa agagaacttg
     3241 aaaactattt agaagtaaac atatctttat taaaagctct tagtcaagat gatagatttt
     3301 ctaatggtga aacatcacta attgaaaagg gaaaattatt aagtccatat caaaaggaat
     3361 ataatctttt tatgattggt ataacagata caaaaggaaa tgcttcaagt acatataggg
     3421 aaaatgttaa ctcaataaaa gataagccta gttttgagaa agcaattaaa acaaagcaag
     3481 tagtagtttc agatattgaa gtcagtaatg taacaggtga caaagtattt atcatatatg
     3541 taccaattgt aaaaaataat gagatgattg ggaccatttt tgcttctttt tattttcaag
     3601 atgtaaataa tttaataagt agaagcaatt ttgatgattc tattaaattt ctaatgatag
     3661 ataaagatta tactattatt tctcatccaa acaaaaaata tgtaaatgat aaaagtaaga
     3721 tgcttgattt agaagggaat attataggaa ccactaaaag tgaaatctta aaaaatataa
     3781 ataaaaaatg tcaaggtgat tttttatctt gggataattg gagattatat aatgtaaaat
     3841 atacgaacat aaagtggact aattggactt tagtatcaaa atgtaatata tttaagaact
     3901 ttaaaaatct aatagttaat tttatgataa agttgtattt ttatatagta atatttatga
     3961 tattgtggaa attaagtaat gctaaattaa tagaacaact taaaaaattg gcttattatg
     4021 attctttaag tggaataaaa aataaagaaa agtttagaaa agattctatg tatattttaa
     4081 agaattatta tcaagataat ttttacttag tacaattgga tgtcaataaa tttaaatata
     4141 taaatgagat gtttggatat gctgaaggga ataagatatt aattcatata tcacaggttt
     4201 taaataataa tacaaataaa tatgaaattt gtgctaggat ggataatgac cattttatat
     4261 tgttgattgc ttgtaacact gaagatgaac ttttaaatag attatctaaa ataaataaag
     4321 aaatttgtaa cttaaatact actaactcat ctaagtataa aattgttatg tcaagtggaa
     4381 tatataagat tactaaaaag gatgatatta aaaagattga cttgttaatt gatagagcaa
     4441 atattgcagc aaaaagtaaa aaggaaaaat atgaacattc atattcattt tttaatgaag
     4501 atacaagaaa tcgattgtac aaggaaaaac gattggaaga taatatgaat aaagctttag
     4561 aaaaaggtga atttatagta tattatcaac ctaagtatag tttagatgat gtaaatgaaa
     4621 ttgaaggagc agaagccttg ataagatgga atagtccaga atttgggttt atttcaccta
     4681 tagattttgt acctttattt gaaaagaatg gttttattgt aaatattgat atgttcgtgt
     4741 ttgaagaagt atgcaaaaca ttaaacaagt ggataaataa agggtatact cctgtaccaa
     4801 tctctgtaaa tatgtctaga gttcatttat atagggataa ttttatagaa aatataactg
     4861 atttgataag taaatacaat atatctcctg agtttattga acttgaacta acagaaagtg
     4921 ttgtgtttga taatttaaat atattgattg atataatgaa aaagattaaa gagataggat
     4981 tccttatatc aatggatgat tttggttcgg gatattcatc tttaaatctg ttaaaagacc
     5041 tttcttttga tattttaaaa ttagatagag gatttttaat cgaaacaaca gatacaaaac
     5101 gtggaaaaat tattatttct aagattgtgg aaatggcaaa agcgattgat attaaagtaa
     5161 tctgtgaggg cgtagaaact tatgaacaag tagagttctt gaaagagata ggttgtgata
     5221 aggttcaagg atacttattt gctaaaccta tggtattaga tgaatttgaa aaacacttaa
     5281 actttaaatt tgattaatca ttaataatta ttagactata atatacccta acaacttact
     5341 tacaaatatg taagttaatt gttagggtat ttttatggtt aaagtagtat attcacgata
     5401 ttatggtatt gtagtaaaaa taaaaaatta tggagaatat aaaatgaaca tattgagtat
     5461 tagaaatata tcaaaaacat attgcggaaa tattccattt aaagcattag ataaggttag
     5521 tctaaatata gaaaagggag aatttgtgtc tgttatggga ccatctggaa gtggtaaatc
     5581 tactctttta aatataatat ctacagttga tagacagagt gaaggtgagg ttgtattaga
     5641 tggatatgat gtaagtaaat tgaaaggaga aaaacttgct gaatttagaa ggaaacaact
     5701 tggttttgta tttcaggatt ttaatttgat tgacacttta actgttggcg aaaatattat
     5761 gttgccactt acactagagg gggcaagtat caaagatatg aatatacaaa ctaaaagtat
     5821 atctaaattt ttaggaatag ataaaatttt agatagaaaa acctatgaaa tttcaggagg
     5881 tcaagctcaa aggtgtgcta ttgctagagc tattataaat aaacctgcca tacttcttgc
     5941 agatgagcca actggaaatc ttgattcaaa atctactgat gatgttttga aattatttac
     6001 taggataaac aaagaacaaa aggtaacaac acttatggta actcatgaag catatagtgc
     6061 aagttattca gatagagtaa tttttataaa agatggttgt atatatacag aaataaaaaa
     6121 atctgaaagc agtaattctt tctattctga tatactagct gttttgtctc aaataggagg
     6181 tgtaagatag tggattttag aaagtttgct tataataata tagttaaaaa tttcagggca
     6241 tatttagcat attttttaag ttctgttttt tctataatga tattttttgt ttttgcagtg
     6301 tctatgttcc atccaattat aacaaaatct ggaatgcaga gtggttctac tgcatttatg
     6361 gctcttatga cttcagaatt aataatattc ggttttagtc tactttttat attgtactct
     6421 ttaggaaatt tcttgaaata tagattgaaa gattttggag tattgatgat aattggaatg
     6481 agtaacagac aactgaaaaa attgatatta attgaaaatt tgataatagg atttttagca
     6541 gtgatgttag gaatattact tggtttatca atttcaaaat tgtttttatt gtatttaagc
     6601 aaactgtttt atatgaattt aactgaattt tattttccag taaaagcaat gctattgact
     6661 attgtatcat ttatgatttt atttttgata acaggaccaa tgagcttaaa attattgagt
     6721 aaaaagagca ttttagagct tttagtaggt acaaaaaagc ctaaaaaaga gataaaatct
     6781 tcaaagctat ttggaataat tggtgtaata tgtcttatat taggatatgc tatgatacta
     6841 tttggcaata agttaaaaat aaatggaggt atgtatacaa cagcactatt aattactttg
     6901 gggatatttt tatttttttc tcaatttact attttgatat taaagtattt aaaaaataaa
     6961 gaaaaatttt ataagaacaa agtaaatatg ttacttataa gtaatttgat atataaatta
     7021 aaggataata cacgattgtt atttttaata acaatacttt tatcggctac tttagtagca
     7081 tttacaacaa cttcaacact tgtaagttct caaggagaag cttctaaaaa taattttcca
     7141 atggtttact catatttttc agagcataat aatgaaaaag aatatgaaga actacaacaa
     7201 attagagaag caatcaaagg atatgattat aaggagttaa gttttcctgt acttaaatat
     7261 aatagggaag tcttactaag tgtaaagaat tataacaaat tatcaaagga attgggatta
     7321 gataaaatta gttcaaagtt agaaaaatat gaaggggtta taattcctag tagcaattca
     7381 caagaacata taaattcttt aaaacaatta aaaaattata gtataaaaaa tttaaatata
     7441 gaagtaaaaa aatcaaaaaa taaaataatt tttcctacag gactgtttag taaagttgta
     7501 gttataagtg atgacttata caatgaagta aaaggtgaat tttcaaaaat taattttcat
     7561 ggatttgatt ataaaaactg gcaaagcagt gcacagataa ctgaaaattt aaaagaaaaa
     7621 cattttaatt tggatagaga atataacagg tcatattttt taacattacc agatatgtat
     7681 ttagtagagt tacaacaaag taaaattctt caatttatgg gtgtttttat aggagtgata
     7741 ttatttatag cagtttttag ttttttatac tttagattat atacagacga gatagaagat
     7801 aatgtaaagt acaaaaaact atcaaaaata ggtctatctt ttaaaaatat gaataagatt
     7861 gttagtatag agataggtac attgtttttt ataccgtatt ttatagcaat tataaatagc
     7921 ataatttctt tactgttttt aaatcaagta ttaaataata gttttaagtt agaaaatttg
     7981 ggtatattac tactgatatc atctatatat tcactttatt tttacttatt aaaaaaaatt
     8041 tatactagaa aagtttggtt ttttgattaa agtggtatat aatagatata taaattaata
     8101 tttaaaaagt agttaatatt aggggagggc atttgcacct cccttgtaat atgtggaaaa
     8161 ttgaatatta gaagaaggag gagacttgat ggaaaatcaa caattattag gaacagaacg
     8221 aattagtaaa ttactactaa aatattctat accagctata ataggtatgt tggtaaatag
     8281 tttatataat gtagtagata gaatttttat tggaaatata ccaggggttg gaccacttgc
     8341 aataacaggt cttggtgtaa caatgccaat aatgacaata atacttgcat ttgggatgtt
     8401 gataggtata ggaactacta ctaccatatc aataaaactt gggcaaggta aagtagaaga
     8461 ggcaagaaaa cttataggaa atgcaatgac gttatctgta ataacaggaa ttatcataat
     8521 gatacttgga atattatttg caaataaaat acttacattg tttggagcaa gtgaaaatac
     8581 tttaatatat gcaaaatcat atatcaatat tatattatta ggtacagtag taaatcttct
     8641 ttccttttct ttaaaccact caataagagc tgatggtagt cctaaaattt cagcaggaat
     8701 aatgatagta ggatgtttaa ctaatatagt tttagactgg atattaatat ttggattcaa
     8761 tttgggaata caaggagctg caattgctac agtaacttct caggctctta cagctatttt
     8821 aactataggg tattatataa gtggaaaatc aaacttacga ttcagtaaat ctaatctaaa
     8881 attagataaa aagctaataa aagcagtgtt tgcaataggt atgtcgccat ttgctatgca
     8941 acttgcagca agtttagttc aagtaatatc aaatattgca cttaaaactc atggtggaga
     9001 tttagctata ggggctatgg ctactatttc ttctattgca atggtatttt tgatgcctat
     9061 ctttggaata aatcaaggag cgcaacctat tataggtttc aattatggag ctgaaaaata
     9121 tgatagggtt aaaaaagcat atttaggttc attagtagta gctactataa ttttatgtat
     9181 gggtatggta gtagttatgc ttttcccaga aactattata ggtatattta ataaagaccc
     9241 tgaacttatg aatatatctg ttaatggatt aagaatatac ttacttatgt tgccaattgt
     9301 aggcttatca gttacaggaa caaactttat acaatctata ggaaaagcaa aaatggctat
     9361 gttattgagc cttttaagac aagtaatttt attgatacca gcagtattaa ttcttcctac
     9421 attcttaggt ttacagggtg tatggacagc tcaaccagtg tctgatttta tagctacagt
     9481 aattacaggt atagttgtat ttagagaatt aaaaagatat actcctaaaa ctgaaaaatt
     9541 aaatgaaaat gaaagactaa acgagataac tactgagtaa aaatataaat tcagataatt
     9601 gagcaagaaa ttgcaaaaaa tacgattata tgataaaata aaaccgctgt caaggataag
     9661 agaaattcta attatggagt gattttatgg aatttacaaa gaaaagcaaa ttaaaaaata
     9721 tgtatgtaaa catgaatgta gctagaatgt tgtctaagat aaatgagtat aagggtagac
     9781 aactgcttta caaaaaacaa ccaaaagaaa ttttagaaaa cttagaaaaa aaatctttag
     9841 tagattgttc tgaatctaca tacattggaa atcaaagaga tagtagtaat tttaatctag
     9901 aaaagttgat aagtaatgaa gttacaccta gaagtagaga ggaattgagt attgttgaat
     9961 acagagatgt ggtaaaaact attaacagtg cttatgagag tattcccatc agttcacaaa
    10021 cgattcttga gcttcatgga tacttatata aattttcatc tactagagga ggaagttata
    10081 agtcagataa tgattttatt gaacacaatt taaaacctag cgaatttata agtattgatt
    10141 caagtgtcaa taaagttgaa aaagctgtag aagaaatatg tgaagcttat aatacattga
    10201 tagaggaaga tgaaattgat atattaatat taatttctgc atttgtgtta gatttcatat
    10261 tgatacatcc attcaaagaa ggtaatataa aaatggcgag agtacttatc ctattgttac
    10321 taaataaaaa tgggtatgaa gttggaagat atataagttt aggaaagata tttgatgata
    10381 gttcatatga atattatagt aatttaaatt atttgaaagc ctcaataggt agtgaaaaag
    10441 cagatatgaa tgcatggatt gagtattttt tagagacgat attaactgca tatgagaagc
    10501 tggatgatag tttaaatata tctgataaga aaagacaaac aaaaactagt agaatagaga
    10561 aaataataaa ttctaccctt ggatatttta caaaagaaga tataagagat ttatgtcctg
    10621 acattccaga acctactata aatagagttt ttaataatct aagaaaacaa gataagatag
    10681 aagttgttgc aagaggaaga agtgcaaaat ggaagaaaaa atattaaaaa atgtttagat
    10741 aactaaaatg gtatagtgtt ttacactata ccatttttaa tgtcattagc atgagcaata
    10801 atttaacaac ttaagcaata tcaattttat ttattgatta tttttgaact gaaaaatggc
    10861 gttatatggt atacctcagt ggtaattaat tttggatgag aagatataat attacagaat
    10921 atattaggag tatatgtaga aaaaatataa taaaagtgtg aggcttagta attaacaaat
    10981 tatgtggctt cacacttttt tataaaataa agatacttag cagaagaata ttttagttga
    11041 tatgtttata agatggtact tgtttgggta aaagtataaa tagtaaaaaa gtagaagtaa
    11101 tgtgtataaa aacgtaagtt acattctgta ataatagtta ttttataatt caacaaaaga
    11161 atagaatggg tatatttgtg aaataataat ataaaataat cttaggagtg agcttatgaa
    11221 gaaaaaatta ttatatataa atgtaaattc aaaacctgaa gacttatctt caagtaaaac
    11281 tgtagcaaga aaatttataa ataaatttat ggaaaaaaat aaagattttg aggttgaaga
    11341 gatagacctt tataaagaac atataccaag acttgagtat cagtactttg aaaaaagaaa
    11401 tagcatagtt agtgaagaaa atgctaaaaa tttagacgat aaggatagaa aagaacttac
    11461 aaagattaga aatctttgtg accaatttgt gagtgcaagt gtatatgtaa tagcagcacc
    11521 tatgtggagt ttatcttttc cagcaccact taaagagtat atagattgta taattcaaga
    11581 tggaaaaact atatcttttg aaggcaatga taaaccacaa ggaattttaa atgatattga
    11641 tagaagtatg gtatatatac aatcttctgg tggacacata ccatgggtgt tgaagccagt
    11701 tatgaataaa gggttaaatt atgtggaaag tattatgaaa tttataggaa ttaaaaaatt
    11761 tgatgaattg ttagtagatg gaactggaac tagtgaagaa gaaagacaag ctgctataga
    11821 aaaagcagct gaaaaaatag atggtataat agatgggatg aaattttaat tataatattt
    11881 tattaaaaat ataaaggaaa aacctctttt aatgtagaaa cttatattga aggaggcgag
    11941 aatcatgcga gaagaaaaaa gtaatgaaaa gtatgattgt tattggtgta atcaagagaa
    12001 taatttctgt gtagaaataa aagataatat agtcatgata gatgatggca ctggtacgtt
    12061 aaaacaagcc gttttcatag ggtataaaca aatccaaatt aatttaaact gttcacattg
    12121 tcaaaacttg aatagaataa aattaaattt gtagaatttt aaaatggaaa ttattgaagg
    12181 aattaaaaat agaaagtgtt aatatttaga ctgaaaatta tattttatac aataaaagtt
    12241 tatttaaata acattgtatt ttaatattta ttttcagtct tactttatta aaattatttt
    12301 ttggttatgt ctggaaaata tctgtctact aatttttctg aaatattttt taattggtca
    12361 gccattgaag tcagacttaa taaggctatc aataaatcat ctttattttc tggatttata
    12421 ctaatatttt caaaattaga ctctataaag tccggtatat tctttctttg attttctttt
    12481 attaacatga atttttcgta acatgaaact aaatcttttt cactgaaatt atccttttca
    12541 tacacaccat gtaagattct tttaatttct tgtattgtaa gtgtattttt catagaataa
    12601 ataattatca tctgtaaaat ttgttcttta gtatactttt ttcccttaac tggtttgatt
    12661 aatccctctt tgctgtaatt atttatcatg gttttagtaa gaattttatc actttcaaat
    12721 cttttattgg cagaaagctt attgtcaatt agagaaataa tttgatccat atataagtca
    12781 atagatggaa tatcgtttga agttatatct gcattattta gtgtttctag tattatagta
    12841 ttaagttcat tattcatcat atgttacctc cataatatat ataatacact aaattagaaa
    12901 gtaaaacaat attaaggctt gtaaaaaaaa cataaagtgg tataattaaa tatataatat
    12961 agtaatggaa actatatttg tgaatctgat ttttaaaata cataattgta ggaggacgaa
    13021 atgtatcaat tttttttaaa gggtagagac ccaataagca gtttaactca ttttataggt
    13081 gcttgtttat ctcttttagc aactataatt ttagtttttc aatctgttac attacaggaa
    13141 acttctctct tgatgatagt atctgtatcg gtatttggat tttcactaat tgctttatat
    13201 agcgcaagtt cgtattatca ttttttaaag gggacaccag aacaagaatt gttttttaga
    13261 aaagtagacc atgcaatgat ttacgtattg attgctggct cttatacacc aatatgtctg
    13321 aattttatgg aaaaaaaaga aggcataata tttgttacag caatatggat agttgcattt
    13381 attggtataa taataaaaat attttggatg gatgcaccta gatggttatc taccagtata
    13441 tatttactta tggggtgggc aattgttttt gatataaatg cattcaattc aattcctaag
    13501 gattgcctta gactattaat tatggaaggt gtatcttact ctataggtgc aattatttat
    13561 ataattaaaa agcctaatat aagtcctgag tttggatttc atgaaatatt tcatattttt
    13621 attatgatag gttctttatt tcacttttta gcagttttat tgtatgtttt atagttttaa
    13681 aagatttgat taattgatac taaaattcta caatgaataa aatggaactg ccttatatag
    13741 attatatctt cgacaataag acagttcctt attataaata ttaaattcta cttataataa
    13801 aattttgata aaaatagtcc attcaaaatg cctattccat aattccttta atgtactttt
    13861 catttacgac atatagtttg aatttatgta ctataattat atcctctaaa ttctctgaaa
    13921 cactaacaat tctgacacat ttgtttctaa tttctacagg taaatctaaa tcatttgtta
    13981 aattcacata gtcatcatat ttatacataa aatcatccct ttacattaag atatacttat
    14041 tatactatat tttaa
//
LOCUS       DAMJZE010000101        13918 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJZE010000101 DAMJZE010000000
VERSION     DAMJZE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.102"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            281..1474
                     /gene="nifV"
                     /locus_tag="PC541_002493"
     CDS             281..1474
                     /gene="nifV"
                     /locus_tag="PC541_002493"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2839949.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1499..3424
                     /locus_tag="PC541_002494"
     CDS             1499..3424
                     /locus_tag="PC541_002494"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2839950.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3446..4441
                     /locus_tag="PC541_002495"
     CDS             3446..4441
                     /locus_tag="PC541_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2839951.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4766..5239
                     /locus_tag="PC541_002496"
     CDS             4766..5239
                     /locus_tag="PC541_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2839952.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5273..5767
                     /locus_tag="PC541_002497"
     CDS             5273..5767
                     /locus_tag="PC541_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2839953.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5864..6385
                     /locus_tag="PC541_002498"
     CDS             5864..6385
                     /locus_tag="PC541_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2839954.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6574..6885
                     /locus_tag="PC541_002499"
     CDS             6574..6885
                     /locus_tag="PC541_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2839955.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6913..7599
                     /locus_tag="PC541_002500"
     CDS             6913..7599
                     /locus_tag="PC541_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2839956.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7831..8715
                     /locus_tag="PC541_002501"
     CDS             7831..8715
                     /locus_tag="PC541_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2839957.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8814..9989
                     /locus_tag="PC541_002502"
     CDS             8814..9989
                     /locus_tag="PC541_002502"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2839958.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10117..10971
                     /locus_tag="PC541_002503"
     CDS             10117..10971
                     /locus_tag="PC541_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839959.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            11015..12250
                     /locus_tag="PC541_002504"
     CDS             11015..12250
                     /locus_tag="PC541_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2839960.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12351..13292
                     /locus_tag="PC541_002505"
     CDS             12351..13292
                     /locus_tag="PC541_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2839961.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13511..13864)
                     /locus_tag="PC541_002506"
     CDS             complement(13511..13864)
                     /locus_tag="PC541_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2839962.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         5497 a         1376 c         2620 g         4425 t
ORIGIN      
        1 aaggtttttt gaaaataaat ctatttaaaa attaaattaa aatgcaagaa aaacataata
       61 actttcattt ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata aatgatacaa
      121 agtaatataa tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa atgaaattaa
      181 aatgaataaa aaataaaaaa ataatcataa gttagaagta tcaatgaaaa ataagactta
      241 atgtttttaa aattaaaagt atactaaggg gggcattaat atgtgcgtta tatcaaaaga
      301 tagagcaaag gaaataaaaa tagtagatac tactttaaga gatggagagc aaacagctgg
      361 ggttgtattt gcaaatagag aaaaaataat gattgctgaa atgttaagtg acctaggtgt
      421 tgaccaaatt gaagttggta tacctactat gggtggagat gaaaaaaatg taataaagca
      481 catatgtagt agaaatttaa aatcagatat aatggcttgg aatagagcag taataaaaga
      541 tgttgaagaa tctatttctt gtggtgttga tgcagtagct atatcaatat cagtatctga
      601 cattcatata gaaaataaat tgagaacttc tagaggctgg gttttagaaa atatggctaa
      661 aacagttgaa tttgcaaaga aaaatggatt atatgtttca gtaaatggag aagatgcttc
      721 aagagcagat atagacttct taactgaatt tataaatgtt ggaaaacaag caggggcaga
      781 tagatttaga tactgtgata cagtaggagt tatgaatcct ttttctataa agaatgcaat
      841 agaaactctt tatgaaagaa ctaattttga tatagaaatg catacacata atgactttgg
      901 gatggcaact gcaaatgcac ttgcaggaat agcagcagga gcaaattatg ttggtgttac
      961 agtaaatgga ttaggtgaaa gagcaggaaa tgcagcatta gaagaagttt taatggcact
     1021 taagtgtgtt tataaatgtg atttaaataa tatagatact agaaaattta gaggaatatg
     1081 tgagtatgta gcacaggcat caggaagaat acttcctaca tggaaaccag tagttgggga
     1141 taatatgttt atacatgaat ctggtataca tgcagatgga gctttaaaag acccacataa
     1201 ctatgaacca tttgacccaa gtgaagtaaa tcttgaaaga aaaatagtaa taggtaaaca
     1261 ttcaggaaga gctgcagttg taaataagtt gagtgaatat gaaatgtata tttctccaga
     1321 aaacgctacc aagctattaa atgctataag agctacatct ataagattaa aaagaagttt
     1381 aatggataaa gaaattcttc aattatattg tgatatacta gcacatgaga aaggcacaac
     1441 agaagaagaa gcagttagag gttcatatat ttaagatata attgtttagg aggaaaatat
     1501 gggagataat atagtttata aaataattaa aaaacatata gtagatggag aagctgttgc
     1561 tggaagttca attggtataa aaatagacca aactttaaca caagattcta ctggaacaat
     1621 gacatatctt caactagaag ctatgggaat agataaagta aagactaaaa gaagtgtggc
     1681 atttgtagac cataatatgt tacaacaagg atttgaaaat gcagacgacc ataagtatat
     1741 acaaacagtg gcagataaat atggagttta tttctctaag ccaggaaatg gtatttgtca
     1801 tcaagtattt ttagaaagat tttcaacacc aggagataca cttttaggtt ctgatagcca
     1861 cacacctact gctggaggtg tgggcatgat ggctattgga gctggtggat tagatgttgc
     1921 tttagccatg gcaggaggag catactatat aaaagcacca aaagtctgta aggtgaatct
     1981 agttggaaaa ttaaataata tggtatcatc aaaagatata atattagaag tgttaagaaa
     2041 acaaactgta aaaggtggag taggtaaagt ttatgaatat ggtggagaag gtgtcaaaag
     2101 cctatctgtt ccacaaagag ctacaataac aaatatgggg gctgaacttg gagcaactac
     2161 ttcaatcttc ccaagtgatg aaaaaacact agaatttttt aagagtcaag gaagagaaga
     2221 cgcatgggta gagctaaaac cagatgcaga tgcagtttat gatgaagaga ttactataaa
     2281 cttggatgaa ttaaaaccat tagctgcaaa acctcatagc ccagacaatg tagatgaagt
     2341 tgaaaatata ggcaagatta aaatagacca agtggcaata ggaagttgta caaattcttc
     2401 ttatgaagac ttaatgaaag tagcacaaat cctgaaagga aataaagtac ataaggatgt
     2461 gagtttggta atagcaccag gttcaagaca agttatggag atgattgcta gaaatggggc
     2521 tttggcagat ataataagtg ctggagctag aatactagaa aattcttgtg gtccatgtat
     2581 tggtatgggt caatctccag gtactgactc tgtgtcttta agaactttca atagaaattt
     2641 ctatggtaga agtgggactt tgtcagcaca agtatattta gtaagtccag aagtggcagc
     2701 agtaagtgca atcaagggag tgcttacaga cccaagagaa tttgatatta aatttacaaa
     2761 tttagatgta aatgagtttt tgatagatga tagcatgata attaaacctg ctgatgtagg
     2821 aagtgatgta gaagtagtta gaggacctaa tataaaacct ttccctctta atacagagct
     2881 aagtcagtct ataggtggaa aagttatatt aaagacagaa gataatataa ctactgacca
     2941 tataatgcca tcaaatgcta agttacttcc ttttagaagt aatataccgt atcttgcaaa
     3001 ctattgtttc aatactgtag atacagaatt tcctcagaga gcaaaagaca ataatggtgg
     3061 atttatagtt ggtggagata attatggtca aggttcaagt agagaacatg cagctttagc
     3121 accattatat ctaggtgtta aaggggtaat agttaagagc tttgcaagaa tccacaaggc
     3181 taacttgata aatagtggta taattccaat ggagttttgt gatgagaaag attatgaaaa
     3241 tatatctctt ttagataatc tggagatacc aaatatttta gacaatctag gtagtggaat
     3301 tttagaagtt aagaatacaa ctaaaggaac ttcttttaag gtcaaagtag aattgtcagc
     3361 aaaagaagtt gatgtattaa aagctggtgg aaaattaaac tacactaaaa atcaagctaa
     3421 ttaatatgaa aagagggaat attgaatgta taaagtgaca cttataccag gagatggaat
     3481 aggaccagaa gttgcgaaag ctatgaaaaa ggtagtagaa gctactggag tagaaataga
     3541 atgggaagaa gttaatgcag gagaggcagt tattgaagag tatggtactc cacttcctga
     3601 atatattata gatagtataa aaaagaacaa aatagctata aaaggcccaa ttactactcc
     3661 tgtaggtaaa ggatttagga gtgtaaatgt aactttaaga caggcattag atttgtatgt
     3721 caacttaaga cctattaaaa gttttaaggg gataaaatca agatatgaag atgttgattt
     3781 agtggttgta agagaaaata cagaagattt atatgctgga atagaacata aaataggtga
     3841 ttatgcagct gaaagtataa agataataac aagaagtgca agtgaaagaa tcgttgattt
     3901 tgcatgtaac tatgttaaag ataataagag aaaaaaagtt acagctatac ataaggcaaa
     3961 tataatgaaa atgtctgatg gattgttttt agatgtgttt agagaagttg catcaaaaca
     4021 tggtgtagaa tatgatgatt tgattgtaga tgcagcagct atgaacttag ttttaaatcc
     4081 agaaaattat gatgtaatgg taatgccaaa tctttatgga gatatactat cagatttagg
     4141 tgctgggctt gttggaggtt taggtataat tccaagtgca aatattggta aagattgtgc
     4201 gatatttgaa gctgttcatg gaagtgctcc acaaatagca ggtcaaaata aggctaatcc
     4261 aacagcactt attcaatcat cagtaatgat gcttagatac ttaggtgaat atgaaaatgc
     4321 acaaaaaata gaaacagctt tagaaaaagt atttcttgaa ggaagtaaat tgactgtaga
     4381 tttaggtggc tcagcttcta ctacagagtt tgcggatgag gtgtgtaagt atattgtata
     4441 gattttattt agggaaaaag taaaatatag attgaagaaa agaggctttc tcaaaataga
     4501 gataagcctt ttttattgga atgataaaat atatttgatt ttatacaaaa gagagttcct
     4561 cataaacgaa aaagttaatt ttgggatatc cattttaatt ttaaaattaa ttgattaata
     4621 cgcaaagaaa gattaaataa atttattatt tttaattgaa ctttcaacca aaatatggta
     4681 aaatttaaaa agttgaactt tcaactaaaa atgaaaatat tgattatgtg aaattctata
     4741 aaacagaaac tatttgactt tagccatggt agtttcagtg gaggtgtgtt tgattaagac
     4801 tttagatagt aatatattaa gagaagtagg tactttatct agagcagtaa attcaataaa
     4861 tgatattaag tacaaggaat taaagttaca aaaaggacaa tttacatttt tgacaagaat
     4921 ctgtgaaaat cctggtataa atcttgtgga attatcaaat atgttaaagg tagacaaggc
     4981 tacaacaacc aaggctatac aaaaattaat aaaggcagga tatgtagata aaaagcagga
     5041 taagtttgat aaaagaggat ataatttaac tccaactgat aaatctttgg aagtttatga
     5101 gttgataatt gaagaagaga atagaagtat agagatatgt tttgataatt ttacagatga
     5161 agaaaaacaa gtggtaacta agttattaga aaaaatgagc aaaaacgtag aaaatgaatg
     5221 gtttaaagta aagagatagt aaataaatac attaacttaa ggagatattt ttatgattag
     5281 aaaagcgaat atgaatgatt tagaaagcat tatgaaaatt ataaaatcta cagtggaaga
     5341 aatgaagact tataataata cacaatggga tgaaaactat ccattagaaa aagactttgt
     5401 ttcagatatt aagaaacaag atttatatat ttatgaagtg gatggagaag tagcagggtt
     5461 tatatgtctt aattatgaag agccagaaga atatttaggc ttaaattggt catctaataa
     5521 aaaagcaatg gtaatacata gaatggctgt aaatccaaac tttagaaaga caggaatagc
     5581 atctaagtta gttgattttg ctgaaaaatt agctgtagaa aacaatgtat catacttaaa
     5641 gagcgataca tattcaataa actcaaaaat gaatttatta cttacaaagt gtggttttat
     5701 aaaaatcgga gaaatgagtt ttttaggaaa ggagaaatct ttctactgtt atgataaaat
     5761 attataaaga gcatttataa tacaaggggt aaataataga ttttcagcat tagtaagggc
     5821 ataatataac ctagtatatg ctattatgaa agggatgatt tatatgattg gatttctaaa
     5881 gaaaagaaga agtattagaa aatacaagga tgttgaagta gaaaaagaaa aattagataa
     5941 aatattaaaa gctgctcttt tagctccatc atcaaagggg cttagaacat gggaatttat
     6001 agtggtagat gataaagaaa agctaattaa tttatctcaa tgtagaacta aaggtggtgg
     6061 atttttctta aaaaatgcac ctttagctat tgtaattatt gcagataaag aaaaaaatga
     6121 tgtttggata gaggatgcaa gtatagctgc ttcatacata caacttcaag ctcatgaact
     6181 aggtttaggt tcttgttgga tacaagtaag aaatagaatg catgatgata atatagaagc
     6241 tgataagtac attagagaag aattaaaagt acctagcaag tacagtgtag aatgtataat
     6301 ttctatagga tatcctgatg aagaaaagaa agcatacggt gactctgatt tagactataa
     6361 aaaggttcat tttaacaact tttagtatat aatatgttgt attaagacat tatatttttt
     6421 tctattgtta atatatactt aaaattgtaa aaatatatct gaattttact aacttatcta
     6481 ggggttatgt acaaggattt taaagataaa tatagaaatt aatgtttata atcgatacat
     6541 aacttgagta acaaaattta aggaggttaa agtatgggta tatttaaaac aaaaatggat
     6601 gaggactgga aagtaaacta cattaaagag tttaatgaaa tgagagattc ttatgagagt
     6661 aaacttcaaa aaaaacagtt tgaggtagat agtttgaaat cagaattgga tagactaaga
     6721 agctataaaa atagccttaa gccaaaagaa aaacaaatta ctgatgatga tataaataat
     6781 ataaagagtt taagaagaga tggcttaagc tataaagaaa tttcaaatca aacaagttgg
     6841 agcaaagcaa ctgtaagtag ggtattaaat ggactttacg attagcatat acatggggtg
     6901 atttaaattg ggatgaagta tagttacaaa aaatgcataa tagattctat tttattgatg
     6961 tttatagttc aaatattaag gatgattttg aattatgtac ttctaagtca atttgaattt
     7021 actcttgaaa attttaatat tataaactta atttctttta cattagttgg tttatcgtta
     7081 attttatttt taaaagataa ttctttgtat aacaaggtta gaaatagaaa aatcacagaa
     7141 gcttttgaag aaaataaaaa caatatactt attgaaaagt gtaagctgat actttttgtt
     7201 gtggttttat ctttagcaat aattgtaact tattgtacta agggatatgt actttttaat
     7261 gtaactatga tgactttgtc tgtattgata gtcccaatat ttgaagagtt attttttaga
     7321 gaatatatat ggaactatct aagtaatttt ataaagtcaa agagtaaaat tatctgtata
     7381 acatctatac tatctggaat ttataatata ggatacatag atgttataag aaattatgta
     7441 atattgtata acaattcaag ctacaccttt gaagttatta tttcaaaaat tatgataggt
     7501 actgtttttg gtattgtact tggattagtt aagtataggt ttagagatgt agggttttgt
     7561 atattattga ggagcttatt tgctatattt attaggtaga tagtataatc ggtttataag
     7621 gttgttttaa atttaatttt aaatcaacct tattttattt gtgttttata aagcacttat
     7681 atattaataa ctaataaatt taataaaaat tatttgaata tttttaaaat atataaatat
     7741 gtattgacat ataaatatta tgaattacaa tttatataaa ctaataaaaa ttaaataata
     7801 ttaatcaact tattgaatgg aggaataaaa atgaaatttg tgactttttg tagtagcaat
     7861 gaagaaaaaa taggtgtatt taattcagaa acaaatagta tatatgaaat taatagttta
     7921 ggattaagta aattatatac tgatatgaat gattttattg aaaacgtatc tacaggagat
     7981 ttagaaaaaa taaaaaacaa ttcatttgaa aacgcaaagt gttataagtt agaagaagtt
     8041 aaattatgtt ccccaattgt aagacctaaa aaagatatta tatgtttagg tctaaactat
     8101 aaagaccatg tcaatgaaat cccagatgga gttataaaaa acgtagttat gccagattat
     8161 ccaatttatt tttctaagag agcagacaaa ataattggag tggatgataa gataagctta
     8221 catggagatt tggtagagaa gttggactat gaatcagaac ttgcagtaat cataggtaaa
     8281 gaaggtataa atatatcaaa agaagatgct tatgaatata tatttggata tacaatagta
     8341 aatgatataa gtgaaagagc acttcaagat aaacatgttc aatggtttag aggaaaaagc
     8401 ttagacacac atacatctat gggaccttgt atagtacata aagaagaatt tgaacatcct
     8461 ttaaaattag atataagtag tgtagtaaat ggagaagtaa gacaagattc taatacagag
     8521 tactttatat ttgatatacc tactataata aatgatttat caagaggaat gactctaaag
     8581 ccaggagata taatttcaac tggaactcca gcaggggttg ctatggggat gaatcctcaa
     8641 gtatatttaa agcatggcga tatagttgaa tgtaaagtcg aaggaatagg agttttaaaa
     8701 aatatagttg attaatggat aaatatgcca tataatttta ttagtacgaa taatttaatt
     8761 aatataggat taatcaaact tatgattaat ttattataga gagggatgag tatatggcaa
     8821 gagaactaga attaaagtat ggctgtaatc caaatcaaaa accatcaaag atttatatga
     8881 agaatggaga acttcctata gaggtattaa atggaaaacc tggatacata aattttttag
     8941 atgcatttaa tagttggcaa cttgtgaagg aattaaaaga agctacaggt cttccagcag
     9001 caacttcatt taaacatgta agtccagcag gagcagcagt aggggtacct ttatcagata
     9061 ctttaaagca aatatatttt gttgatgatt tagagctttc tccattagct tgtgcttatg
     9121 caatggcaag aggagcagat agaatgtctt cttatggcga ttttatagca ctttcagatg
     9181 tatgtgataa ggagactgca actattatag caagagaggt ttctgatggt ataatagctc
     9241 caggatatac agatgaagcc cttgaaatat taaaaggaaa gcgtaaaggt aactataata
     9301 ttgtaaaaat agatgaaaat tatactcctg aacctattga aacaaaggat gtttatggaa
     9361 ttacttttga acaaggtaga aatgaaatat taataaatga agacctatta aaagatattc
     9421 caactgataa taaaattttt acagatagtg caaaacgtga tttaattata gctcttatta
     9481 cattaaaata tacacaatca aattctgttt gttatgctaa agatggacaa gtaatagggg
     9541 taggtgcagg tcaacaatca cgtattcatt gtactagact tgcaggtaac aaagctgata
     9601 cttggtatct tcgtcaacat cctaaagtat taaacttaaa atttaaaaag gatataggtc
     9661 gtccagatag agataacact attgatgtat atctttctga tgattatatg gatgtgcttg
     9721 ctgatggcat atggcaaaac ttttttgaag aaaaaccaga gcctctaact ggagaaggaa
     9781 aaagagcatg gcttaaaact ttaacaggag tagcacttgg ttcagatgca tttttcccat
     9841 ttggagataa tatagaacgt gcaaaaagaa gtggggtaag ttttatagca caaccaggtg
     9901 gttctattcg tgatgataat gttatattaa cttgtaataa gtataacata gtcatggcat
     9961 ttactaaaaa tagattattc catcattaaa atactgaaag tataaagata atatagatta
    10021 agtaaaaatc tacaacaagt tttgttgtag atttttttat ttgctgtata attttaaata
    10081 gaactttatt tttagatatt caatttggag gttgatatgg ctttaaatat aagaaactat
    10141 agcctattat gtaatcaagg aacagctaaa tttaatgaag atgtagtcgg tataaatcca
    10201 tttggggcat gggttttaga tggtgctaca ggacttaatg gaaaaaattt agtgtcaact
    10261 gaaagtgacg caaggtggta tgttcagtgg tggaataggt atttatataa aaatataaat
    10321 aaagaagaat cattaaagag tattattaat actggaataa tagaagtaaa aaaagagtat
    10381 gaactaagat taaatgggtt taaggttgaa aaattagatt taccatctag ttctatagtt
    10441 gttgtaaaat ttcatgaaaa taaagtagag tattttttat taggagattg tgctttgttt
    10501 attaaaaatg gagattccaa agttataaaa gataggagta tttgtaaatt tgataatact
    10561 gtatttgatg aaatgagtaa actttctaac ttggaagaga tggcttttga tgagattaga
    10621 actagtgtta tggatacaat agtaagcaat agattgaaga aaaatacaaa agagggctat
    10681 tggatattgg attttgatga ggaagctgtg gaaaattcta tatatggata cattgaaata
    10741 aaaaaagatt ttcaattgat gctaacgagt gatggtttta caagtgcatg tgatagatat
    10801 aatctaataa aagaagaaga attgatacgt atagcagaaa aatttggtat aggatatata
    10861 cataataaag taagggattt tgaagataat gactataaag cggttaaagt gccaagattt
    10921 aaagtaaaag atgattcatc ttgtttatat ttagatatat ataatgatta gactgtataa
    10981 aactatttaa ttttagaaag gaggcaagtg aaaagtgaaa attcttcata taataacaca
    11041 aaaaccaaat agtacaggta gtggtatata tttgtctggt atgataaaag ggttcgaaaa
    11101 aatcggtcat aagcaagctg taattgcagg tatagatgta aatgatgatg taaattgttt
    11161 tccaagtgaa gtaagttttt atccagtaaa gtataattgt ggggagttaa attttcctgt
    11221 tgtaggtatg agtgattcta tgccatatga aagtacaaga tataaagatt taaatataga
    11281 catgataaat agattgaaat atcaatttaa agtaaatata gataaggcta tgaatgattt
    11341 taaaccagaa cttataatat gtcatcattt gtacctctta actgcatttg ttagggaaat
    11401 ggtaaaagat ataaaggtta tgtctatttg tcatggaact tgtttaagac agttaaatac
    11461 cattgattta gagaaagaat acataatagc aaatataagg aaattagatt taatatttgc
    11521 tcttcatgaa aatcagaaat atgatattat aaagactttt ggagtaagtg aaagtaaagt
    11581 agttgtaatt ggtagtggtt ataatgatga tatattttat aataaaaatt ataaaataaa
    11641 ggatgataaa ataaaaatag tatttgcagg taagatatgt aaatcaaagg gactgattcc
    11701 atttataaaa gctatatcta aattaaaata tagtaaagat ttaatagaag tcaattttgc
    11761 aggtactgga agcgatatcg aatcatataa tgagatagtt aaactggcaa gtaagtctcc
    11821 atttaaaatg aatttcctag gaaagttaga acagagagat ttagctgagc tattcaacag
    11881 gtcacaaata tttgtgttac catcatttta tgagggatta ccagttgtag ttttagaagc
    11941 tttatcttgt ggcacagatg taatcactac tgatattctt ggtgttaaag agtggattgg
    12001 tagtgagatt aataattctg gaaagataga atatgtttct ctacctttta tggagaaaga
    12061 aggtattcca aaagatgaag agttatatga ttttgaaaat aatttgtata atgcaataga
    12121 ttctaagata caatctttat tgaataactc aaataagaaa acatctgtag atatgtcaaa
    12181 aaaaacgtgg gatggcttag catatagaat aaatgaagtt attttaatgg atgaattgtg
    12241 tttagtctaa aattatttaa attataaaaa agtgtaacta tttttacact atttttaaat
    12301 gattttatat tatactaata ctaaatatat ttgttttagg gaggtaattt atgaggatta
    12361 gtaaaaaggt attggcgtta ggtatttcag cattactgtt aagtgttagt tttccaacat
    12421 caataaacgc actagataag attgaaaata ttcaaggagt agataaatat gaaacagctg
    12481 ggcttatttc agataagcaa gattttacaa cagcagttct tatcaatgca gactctacaa
    12541 tggtagatgg gattgctgca agtggtcttg caggagtaaa taatgcagct attttattaa
    12601 ctaataagga tgatatacca gaagcaacac ttcaaagact gaatagagtt actaagatat
    12661 atgtaatagg tggagaaaat tctataagta aagatgtaga aaaaatgtta cttatgagaa
    12721 gaatgcaagt tataagaatt gatggtgtag atagagtaga tacaagttat aaaattgcag
    12781 gtgaaataga aaaaattaaa aacagtgata agatgttttt agtaaatgga tttaaggatg
    12841 aagctgatgc agtaagtgtt gcatctgttg cttatagaga tggagctcct ataatattga
    12901 caaaagatat accttcagca gaagaagaca cagacttaga cccatggttt ggagtttctc
    12961 ctgttccagt atatgctatt ggaggagagt ctacattaag tgattacata gtgagcagat
    13021 atagagcaac tagaattggt ggtgttgata gatatcaaac taataaaaat gttattgaaa
    13081 agttctataa tggagcaaaa gagttctata taacaagtgg agatgattta gtttatgctt
    13141 tagttgcatc accacttgca aaaaatgctc ctgttgtttt agtttcaaat aaaagtgata
    13201 agagtatatt aagtggagct agtaaagtaa cagcaatagg tataagtgat aagagtatta
    13261 tagagcaatg tttggatgct gtaaagaagt aatggagtgt tattagtaaa atgaatatat
    13321 acattgaaaa ttagtagatt gttcatttta taatggtata aaatatatgg gaagaggcta
    13381 tcaaatggag ataagcctct ttttcattgt taaaattatg aaaagatact ttattttata
    13441 taaaaaagta tcttatgaac taaatgtcta ttttgagaca ctatctactt ttcttatatt
    13501 atattatttt ttatatggaa taaatttcat aagtattatc ttcctttaat ttttctaacc
    13561 aaaccatctc taaaatatct agttcttcta aatagtcttt ctctgttttg ttatcatcat
    13621 gttttataag ttctaaaact tcaaatgtaa atgctttttc accatatttt ttccattcat
    13681 tatttaattc ttttatcata catgagccag caccaagctg aaatttgaga gagttaaatt
    13741 tgctttttat gtcattactt tttccaaggt aaatcttttt agtaatatca ttttttatta
    13801 tgaatacacc tgtgtcaaat ttcatttctt tgtataattg ttttaattca cgttttctat
    13861 ccatgatttt agatacctct ctttataatt gatatgtaat atgtaagtgt aaaaatat
//
LOCUS       DAMJZE010000102        13896 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJZE010000102 DAMJZE010000000
VERSION     DAMJZE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.103"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            161..1564
                     /locus_tag="PC541_002507"
     CDS             161..1564
                     /locus_tag="PC541_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2839963.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2041..3063)
                     /locus_tag="PC541_002508"
     CDS             complement(2041..3063)
                     /locus_tag="PC541_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2839964.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3098..4696)
                     /locus_tag="PC541_002509"
     CDS             complement(3098..4696)
                     /locus_tag="PC541_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2839965.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4738..6003)
                     /locus_tag="PC541_002510"
     CDS             complement(4738..6003)
                     /locus_tag="PC541_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2839966.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6145..7572)
                     /locus_tag="PC541_002511"
     CDS             complement(6145..7572)
                     /locus_tag="PC541_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2839967.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7610..8617)
                     /locus_tag="PC541_002512"
     CDS             complement(7610..8617)
                     /locus_tag="PC541_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2839968.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(8814..10136)
                     /locus_tag="PC541_002513"
     CDS             complement(8814..10136)
                     /locus_tag="PC541_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839969.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10494..12134)
                     /locus_tag="PC541_002514"
     CDS             complement(10494..12134)
                     /locus_tag="PC541_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2839970.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12290..12946)
                     /locus_tag="PC541_002515"
     CDS             complement(12290..12946)
                     /locus_tag="PC541_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839971.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(12972..13673)
                     /locus_tag="PC541_002516"
     CDS             complement(12972..13673)
                     /locus_tag="PC541_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2839972.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4710 a         2625 c         1663 g         4898 t
ORIGIN      
        1 gaaatctcta tatacataaa ttcatttttt catacatctt acaaatttat ttttatttag
       61 ataacatgta tattttttcc atgaaaaata aataataaaa aatagtaaat atggaaaata
      121 taaaattgat taacaataat taattacctg aggtgtgaat atgaaaatgc taaacaactt
      181 ttttaagaaa atcaacaatc cagctcctgt ctttgcacag acaaaaatgg aaagagataa
      241 tcaaacagat gattcacttt ctacaaaacc aaagactaca tttagagacg ttgctggatt
      301 agacgaagta aaagaagaac tatttgaaat agtcgatttt atgaagtcac cacaaaaata
      361 tcaaaaaatg ggtgcaaaaa tacctaaagg agtattattt tatggtcctc caggaacagg
      421 taaaacccta cttgcatctg ctgtagctgg tgaaacaaac tcatcatttt tcaatgttac
      481 tggttcagag ttcgttgaaa aatatgtagg tgtgggagca aaaagagtta gaactctatt
      541 tgaaaaagct agaaaagaag ctccaagtat aatatttata gacgaaatag atgctgtagg
      601 agcaaagaga catcttgaaa gtaacaacga aaaagaccaa acactaaacc aacttttagt
      661 tgaaatggat ggatttaata aagattccaa tgtattaata ataggtgcta ccaatagact
      721 tgacttatta gatgaagctt tacttagacc aggaagattt gacagacaca ttcatatagg
      781 tgcaccaaac tatcacacta gatttgaaat tctaaaggta catacagatg acaaacctat
      841 agacaaatct gtaaatctag aacttttagc taaaaaaact catggtttca atggggctca
      901 tctttcaaat atagcaaatg aagcagctat atttgcagtt agagatgaca gtgagtgtat
      961 tacatcagaa cattttgaca aggcacttga aagagttatt gcagggctag aatctaaaaa
     1021 ctctgcattg gttgaaaaag agaaaaaaat agtagcatat catgaagctg gacatgctct
     1081 tgtaagtgat atagtaggaa tttgtcctat acaaaaaata tctatagttc ctagaggaca
     1141 agcattaggt tatgttcttc aattacctga tgaagataga tatatttata ctaaggatga
     1201 gttgattgga aaaataaaaa tattacttgc tggaaaggct tcagaagaat taatatttaa
     1261 tcataaatct actggtgcaa aggacgactt aaagaaagtc actgaaatag ctaaccaaat
     1321 ggtttgtgaa tatggtatga gtaacttagg atttatgact attgatggaa atgataagac
     1381 tttcttatgt gataaggttc aaaaggaagc aaatcgtata gttgaaattt gttataaaga
     1441 gactttagag atgttaaagg ataatcttga agatttacat tctgtttcta aattcttatt
     1501 tgaaaaagaa actatgactc atgaagagtt aaaagatttg ataggaaaag aagctgtaaa
     1561 ctaatatagt tttaaaaatt tgaggctgtc tcaaaataga gataagcctc tttttattgt
     1621 taaagttata aaaatacatt tgattttata caaaaaagtg cctcataaac taaaaatttc
     1681 attttgagac acttacatat tttaattaca tattttaatt ttgtctattt acagaatatt
     1741 atatctacat aaaactaaac tgctgtaaac ttaactacct ccagtaatgt ttgaagtggt
     1801 ataaaagcta gtagcctagt ttctacactt ataatttata ttttacataa ctaaatttag
     1861 tagattttta tactcacaat ttagaacgat tgaaaactta aaaatttctt atcagatatc
     1921 tacaacttaa ttaggtatct atcacttgat taaatatcta tcacttaatt aggtatctat
     1981 cacttgatta aatatctgcc atttaattaa atatctacca cttaattaaa tatctaccac
     2041 ttaaagtgta gaacctcttt caatcaattc aggttttaac atttcaacga ctgactctgg
     2101 attttcttca tttaataagg acttaagtat gttaattgtt ttttcaatca acaaatctag
     2161 tggctgtcta atcgtcgtta aattataact cttccatgaa gccatttgaa tatcatcata
     2221 tcctatcact gataaatcct ctggcaccct caacttgtac tcaaaccttg cagcatccat
     2281 tacaccaata gctatcaagt cacttgtgca aaaaattgct gttggaggat ttgataatgc
     2341 taacattctc ttacctgctt catatccaga atcataatca tagtttgcag attcttcttt
     2401 aaggttatga atcccacttt gtcttaactt agagtaaaat ccaattttct tttccatttc
     2461 ttcacttgtt tctctcgtaa attgaatata tcctatattc ttatgacctt tcttaagcag
     2521 atattcagca gccattcccc caccttcaat tgggtctaca tagactgtac ttatttttat
     2581 accaggtata aaacgattaa aaagaatcac aggaatattg ttttcttcac aagtctcagc
     2641 catcacctta ttcatggccg aagctgtaat aatcacagct tctacttgaa actgaattac
     2701 ttttgaaatt atactatcta tactttgttg tctaggaact ttaaaaacga gacactgttt
     2761 acctatctct tgaatttttt ctacaaataa attgataatt ctattgtaaa atggacctag
     2821 actatcacca actatcaatc ctataacatt agtttttcca ctaatcattc cccgtgctac
     2881 caaattagga acatatccta tttcatttgc tacacgaaaa attctttctc ttacttcagg
     2941 ttttacgccc tttccatttg cactaccaaa tgctcttgaa acagatgatt gagatactcc
     3001 tgccaatctt gcaacatctt ttgaggtagc cgatttatta taatgaactt tcttttgatt
     3061 catttagtat ttctccttaa atatctagta tttcttctta aaattgtatc aaaaataata
     3121 taatatattg gataataaaa aaagctaata caattaattc ttgtttacct aaagaaaatg
     3181 gcactataga tactccatct actttatcca ttccttgaga aataaggtga tttctcatac
     3241 caaatgcagt gtatttttta atatcatctg cattatgatt tttctttaaa atgacactaa
     3301 aaatatatcc aaatatcaat gttattgtag cacctattgc tggattccac aaccaactca
     3361 cattatattt attagaaata aaaaatccta aaactaaccc tacaataaaa ccaatagata
     3421 ctcctttatc attagcttta tatgtaaaca ttgctagtac aaatgcacct gtagctggtc
     3481 cagcaaagta tgagatatag ttaccaacaa ggtcaagaat tgatttgatg cttcctccaa
     3541 atcccataat aataaaacct gtcataacaa ctcctataac tacagttata atcattgtaa
     3601 cttttaatga tgattctttg ttatcttttt tgaaataaaa ctcataaata tccttcgtaa
     3661 aaacagttgt catactgttt aatatagagt cgatactaga cattgctgct gcaaacacac
     3721 ctgcaataat aattccaatg gctcctactg gaagttcatt taaaataaaa gtaatcatta
     3781 tttcatttga agtttcaaat gtccttcccc cataaaaaac aaataaaatt actcctacca
     3841 atagcataaa gtagtaaact aaattcataa caaatgcact aactacaaaa gaattttttg
     3901 cacattttaa agatttagat gttaaaactc tttgtacttg agattggtca aaacaaaagt
     3961 atcttatcca cattacagtt ccaccaatta aagtagccca aaaagtattt gtcactgaaa
     4021 tatccctact gaaatcaaat gtatttaatt ttccactctg ttgtgcaatt tgtaatgtat
     4081 caaagaaact tagattcatt ccttttaagg ctacaataat tactataaat acagcactta
     4141 ttactactat catttgaata ctatcagtcc atataactgc cttaatccca cctaatagag
     4201 tgtatataat tgatacaagt acaactattg ggactaaagc ataaagtgac cagccagtaa
     4261 tcatttgaat aataagcact ggaatatata ccatagaact agcttgaatc aatgaattaa
     4321 taaaaaattg aataatagtt aatcctcttg aataatttcc taaacgaagt cccatatatt
     4381 catatataga ggttattttt aatttataga taactggggt ggtaatgctc agtgcaaaaa
     4441 atattgctaa aggtactgta atattaacca ttactggata cattccatct acatatgccc
     4501 atccaggacc acctataaag ccatttgcac taatcatagt agctgcaact gataaagcaa
     4561 ttggaatcca tggcattgac ctacctgcca agaaataatc ttcttgattt ttattatctt
     4621 ttcctgaata ataaccaata actaacatta atagcaaata tcctattata atacatacat
     4681 caattgatga aatcataagc cacccctttc taagtaacaa aataaattcc tagtatttta
     4741 ttttttatta tttaacgtct ctggtaagaa taaccataca atagcacctg ctacaaagac
     4801 tgcgattgat atgtaaaaga tagtcatcat acctaaagta ccagaagctg taccagctat
     4861 agcaggtatt gcaaacgtag atataatcct tcctccatta aatgacatac cagatgacaa
     4921 cgaacgatat ttacttggga acaactctgt gtagtatgct ccccatgctc cagaatatcc
     4981 aacagcaaca cctagtaata ctgatatcat atagaatgtt gttgtattat ctccacctaa
     5041 ttttgtatat ataaaaacaa atactgctgc taatgccata cctataaaag gtatttttct
     5101 tccaaattta tctgcaattg aaccccagaa taaatatcct actaattgac caattccttg
     5161 taaactatat atagactcag ccactgctgc tgacattcct attcctgtca ctaggtatgt
     5221 agttgcatta tttgaaaatg ctgaatatgc tgtaaaattt gcacctgata aaagaatcgt
     5281 tcccaatcca atccataagt attttccctt atacatactt atatagctaa tcttttcacc
     5341 agtttctttt ccctttttaa ctaattcctt tgtatactcc aaatcatctg gcacaaagaa
     5401 gattgctaag atagctccaa ctataggtgg tataatcgca gtatacatca atatttgcca
     5461 atttgcatca cctaaaacgc catatacttt cccaccaaaa atcattgcta atgaaaataa
     5521 agcagttaat atagcagcta acttaccacg atatttactt ggaaacatat ctgcaactaa
     5581 aggcatagct gttccaaata cgcctcccaa cactactcct gtacacaaac gtgctatttt
     5641 ccataacata atatttgtag gagataacat tggtataaat gttgtcaatc caatcagtaa
     5701 agcaacgcta attaaagtcc tcttcttccc aatttttgta gaaatcaccc caaaaataaa
     5761 tgtacctgga attgtaccaa aagacataac tgcaaatatt tcttctgttt gttttaatga
     5821 aaaaccaaat cccccatctg ctaaagcata aatcattgtt ggtttaataa aagtacccac
     5881 cataaaattg tagcaataaa aaaagtatat taaagcaatt gaaaaaaatg caattcctct
     5941 ttttaggttt gaatagtctt ttcctaatat gatatcattt tctatttcaa ccgttgtttt
     6001 catattttta taattccccc ttttaaattt agtcataatt ttgctaaaag tttaaacatc
     6061 tatttttcat atacattccc cacttaaatt ttataaagca atacttccct atatatttca
     6121 aaactattta tactctaaac agctttattc ttctactccc atataaattt gttttaatac
     6181 atccatacca tcaaaagtta tccactcctc ctttacttta ccttgtagta tatgataatg
     6241 attgattccc attacttcta tatgctttcc agaaggctgt ccaaaaaatc ctataccttc
     6301 atttattcca cgtaatctcc atcttacaga tacatcataa ccttcatttt tttctctttg
     6361 attacaagtt actctttcca cttcataact tccatttgga attgatgcaa ataaacttac
     6421 aatcattcct tgaatctcat catagccatt taaatcttta tcacatataa aatgtactac
     6481 tgcattatca tgatagtatt ttttaacttc atttatatat ttataattat aaatacggct
     6541 tgtcatttca agcatcattt cgccaacaga atcatctttt gcttgatact tagttggcat
     6601 aaattgacca tccatagatt cacagatacc ataagttgat tgtaaactaa gcactttaga
     6661 ttcacttgcc tttgccatac tctttgctac ttcctgaggg tttaatccta attgagtaac
     6721 aatccataaa ttatctctaa ccaaccattc ttcatggatt ctattgttag ttgtggcaca
     6781 atcaattaca gttctaaagt ttatttttct acctgttgct ggtccaaaat tactatcacc
     6841 taagttagtt gctgtagaca aaacacggtg agaagataaa aaaccatcag caccaaagtt
     6901 agaccatatt acattttgac caattaatct tctatcagga aacgcatgta atgtttgaag
     6961 agtattggaa ataacatctt taataccaac taaatttgaa gagccacaat gcattgtaac
     7021 attattgtga tatgtatcat aaataacacc tatacctttt tcttcccaga tattatgtgt
     7081 aatcttcata atataatcta caaaatctct atacttagaa tcaaatccag gtatttcctg
     7141 ttcccttttt gtctgttttg aaaaatcatt gtaatccatt actccaacag gcgttacttc
     7201 taatgaatct ccatagtaaa tttttgtaga tgacatatta tcttccccct taaatataac
     7261 ttttttattt ttttcagtaa gagtatttgt atttaattta tctgtatcca ctgtatctat
     7321 gttaacttta tttatgtcgg atttatttgt gtttgtttta tctatattcc ctttatctat
     7381 atttccttta tctatatttc ttttatatgt gttcgtttta ttctcatttg tttgattttc
     7441 atttatatta ctaaaattag tttgattcat tttagctttg ttttctttaa ccgtacatga
     7501 ttcaagccca cttgtagaaa cttctttttc ttcctcttta cttgttgcat tatttttgtt
     7561 ggtagatgcc actttatatc cccctttgtt ttagaatacg tatgcataac taagcattaa
     7621 aaatactttt agttctctca agaatatcaa gcccttgttg tttcaaccaa taaggaatat
     7681 caattaacac ccagttttct tgaagtttat cgcccttacg ataataaaca tcaacaactt
     7741 gcatatctgc tttaacctct ccacctggta atcctaaaaa acctccacat ggtacattag
     7801 tcagattagg ccatccaaag aaacaagcaa aattaccttc tgcaaatcta catacatgcc
     7861 cattaaaagt ctttcctttt aaattatttc taaatggtag ctggtgttgc tcttgatatc
     7921 taggtatagt ataagaagca ccaattccac ctggaccata ccaaatcata tctttagccc
     7981 aagatttttc aagtatttct ggtgggcatc ccatagcacc actttcattt agttcagata
     8041 aatcatctac cattttattt actaatgcta aagtttttac tccttcaact tcatctgcat
     8101 cttcaaaaag taatccatta tggtcaattg gccctggata tacaaaatat gaacctgttt
     8161 gtggtggcaa tggattaact cctgcttgaa tcataaaccc aattatatct acaaataatc
     8221 cagttttagc aattttacca tcttgaacac agttaaattc tgcatatctt aaactaatca
     8281 ttttacgagt atgtttaatg ccaagccaat cgttatcaaa aagtcccata aaatgcccca
     8341 tactcattac ccattcacta ttgtcaatct catttgttcc tgcaatgaaa atatcttgtc
     8401 gtctttgcat attactcaat gattttttta aaggcttcca aaatacatct gctacatttt
     8461 cagtgccttc ttgttcacga aatggataca cacctttcca gttataatct gatttcatat
     8521 gttgctttaa gacattttca acctcatccc cagtagcatt ttctaatgct tcaaaataat
     8581 ttcttacaac tttttttgaa tcctgatatt ttcccataat tatctcctcc cagatttatt
     8641 taagttataa attaactttc tcatttcatg catacgattt cctttttaat gattttttag
     8701 ctgttttaag tttattttat gaatacatat gcatttttct aaatattaac atatatctaa
     8761 aaaattgtaa agaaaatgtt atcataaaaa tttaattttt gacttatatc tatttaaaac
     8821 aacagaaaag gtttaaaacg taatattcta ctgggtctac catacttgct cataagctgt
     8881 tcactcacta cctcacaata accagaatca cacaatctaa gtataatcct gtccatagtt
     8941 ctaatactta caccacatat agtagctaat tcctttgatg taaattcagt ttttccacta
     9001 tttattattt tattgaatac tttatataca gtattagcac ttagccctgt ctctgtagca
     9061 atttgataaa aacgttcatc aaaactattt ttctctttga cagtaggatt tggctttagt
     9121 ggtcccataa tttctccatt ttcaaaaact acatatgctg catcattttt ttgactttga
     9181 gccatcttaa tactctcata agcgttaaat ttagcttcat ttgctgtttt tccataacca
     9241 attcctatat aagtgtttat aactgaaact tcacgaatca tatccaacaa ataaatatct
     9301 ctgtatttct ttgtttctaa ctcaagtatt tttttagttg taaaaatcat aaattcactt
     9361 ctactctgct ctaccaaaac accatctata cggctattga aatggtatag tttatcaaga
     9421 atcttaatcc tttgtgaaag ataagcgtac tcatcttcct tcattagtga atattctgaa
     9481 ggcatgttca gtttaatagc aagaacaata atctgattct cattgttttt ctttgcaata
     9541 taacgcatct gaatgttttt tatactttgc accattacgt cctcagaagg caaggctaaa
     9601 actactggaa tattatcttt tttcattata tgataggcct cttcaagacc tgtaatacaa
     9661 caacatacat cattattttg ataattatat ttatgaaatt caagcacaaa ctttatatag
     9721 tcctcttcta aatacttttg ctctgcaaaa aacatatgtt catccttaaa ttcaactcca
     9781 atttctttat acaatctctt catcatagcc ttatcataag tatctatact aagtctacta
     9841 atatcgaatt taagcaaata tgtaatttca agcaacactc tgtacaatgt tgtttcatgt
     9901 ctaggaatat aatcagacaa tacaaaatct ttttgtcttt tttcaaaaaa tttaaatggc
     9961 gacttacctg taaaaagaac tgcatccact ctacctctat atttttccaa taaatcaggt
    10021 acttcaatat atctatcata tctaagaaaa tctgcttcaa tattaggaaa attcttttta
    10081 cttactgcta gtattttatc gattaccata cctggtccta cataaagtat tttcatttcg
    10141 tctctccttt cctaatgata ctattatctc ttaaaacatt ttctaaacct accatgacta
    10201 aagccacagg actcctgatt catagaattt tgaataatca attataccta atataaaaag
    10261 ttactttaag ttttttaatt acgactatct gttgaaacat ttttgtattt aaatttttgt
    10321 atttaaatct tattcattaa tattaattac attttacaat taatatattg ttttttctac
    10381 ttaaagttaa attctttaga aaacacaagt agagtaatct ttattaattt aacaaaaata
    10441 gccttcctta atattcaaag aaagactatt tttataaaag ttcttaatgt attctatttt
    10501 gctatgctaa gaggattttt aattacacgt ccagcacata catcagtttg ctttcctcct
    10561 gccaatgcaa ttttaccatt caccaacaca tattccactc ccactgtata ctgtttagga
    10621 ttactataag tcgctttatc ttctattgta tctgggtcaa ataccacaat gtcagcaaaa
    10681 taattttctt taagtagacc cctttcttta taaatgccta agcgttttgc tgaaagataa
    10741 gttatctttt taactgcttc ctcaaaagtc aacatacctt tttctctaac atagtaagag
    10801 aaaattcgtc caaaagtacc aaatgcacga ggatgaggct ttccaagaga aagtaaacct
    10861 tctgtagaaa ggtccatact atcagaacct atacatgact ttggatggca aacaacctta
    10921 tctgtatcct cttgaccttg tgtatgaaca actacttgaa ttttactttt ttcttcaatc
    10981 aaaagcctaa agaacgtatc aaatggattt tcaccattga ttttggctac ctccattaaa
    11041 gttttaccct ctacatattt atttgcttca gtcattacag aggctagtac aacaccgttc
    11101 cacccacctg ctaattggta gaaacattgc catccaggaa tcccttcctc caactctttt
    11161 ataattctgt ctctattttc ttgtacttct aatcgtttta ccatattttc tacaccgcct
    11221 tcaaaagccc aaggaggtaa aagtgcaccc aaagatgtca ttccagctgt atatgggtaa
    11281 caatcaaaag ttacttccaa accatctgcc tcagcttcat ctagcttttt aaatacctca
    11341 tcaatagttc caaaattctt atgacccata acttttaaat gagaaatatg tagaggaact
    11401 cctgcagtct tagctatata aattgcttca tcaagagctt ccatttcttc atcaccttca
    11461 tctctaatat gagatatata gtatccattg tatggtatca attccttaca taattcagcc
    11521 atttcttctt tctttgtgta tgctcctggt aaataaacta atcctgatga aagagctaca
    11581 catccagctt ccatagcttt tctaacttcc aacttcatat ttttaagttg ctctaaagta
    11641 gctggttcct ttgaaaatcc catttcattg atacgtacag gtccttgtgc tacataagct
    11701 acaacattga ctgctggaga attttcagtt aagtaatcaa agtattcttg cattgtattc
    11761 caatgaagct ctaattttgt tggaattgta ccaattatcc tagttccaag ataatcaatt
    11821 aactcttgct tcttctcttc tcttatcgga gcaactccta taccacagtt tgcaataaca
    11881 tctgttgtta cacctgtatg gatacggctt cttgtgtatt tatcatacac aagagataaa
    11941 tcagaatgag tatggaaatc tataaaacca ggtgatacaa attttccagt tgcgtctata
    12001 gtctctttag cctctttttg acttaaatct ccaatagcca tgattttgtc tcctacaatc
    12061 cctacatcac ttttatatct tgaatttcct gtaccatcaa cgattattcc attgacaatt
    12121 ttaacatcaa acatgctttt tctcctttca agacttattt atattcatat ttcttaataa
    12181 acaatcctat tcttttttct caacaaataa ttctagtctt tctccttaat aaacaatcct
    12241 attctttttc tcaacaaata attctagtct ttctccttaa taagcaatcc tattcttttg
    12301 ttttattgtt atcaattaca aagaagaccc ttgtgataag tatcccacct aacacaataa
    12361 acaatgttaa taatccacct ggtataccaa ttttttcacc aaagtacata attgctaacg
    12421 ctacagcaat cgtacatcca ccagctcgaa tatccttacg tgcaatatca acataaattg
    12481 ctccaaataa tgctggaagt atataattaa atgaattagt aacaaattca ggtaacaaag
    12541 gaattacttt atagcctaca atggtaaaaa tggtaatcat agaagctgat acaaagattg
    12601 aacaacatgt accaattgtt ccaacaactt caccctcatg agttcctgct tctacacctg
    12661 caattttctg tgccattgat gctgcaggca tacgtatatc tccaacagaa cctgcaagcc
    12721 aacctatata agaacctgga gctcctagtg taggataata agctataggt tgaacaatcc
    12781 aactaacccc ataagtcgca gcgactaacc cccaaatttg aaatataaca gaaagcattg
    12841 gcatctgacc aaagcgaata cctacataga tagcgggaat aaagtttgca ataattgcaa
    12901 gacctataga aattctccct atttttatca tctttttatc atacataatt atcacctctt
    12961 atccgtttta atcacgataa tataatctgt gtaccaaaca taccaattaa catggcaata
    13021 cctaaggata attcttgtaa tcgttgattc tttttaaata aagttgtcaa tactagcata
    13081 actatagctg aaataattgc tggtgtaagt tttggtattt caaatgtttt tatttgattt
    13141 gaaagaaggt aagcaaataa tccaactgta gcacctgcaa gtagtttttt aacccaagta
    13201 ggattatact tcatgttcat cttttcaaca attccacgca ttctatgacc caaaataaat
    13261 acaacaaata gccaacctaa gttatttaaa gccatccccc atagtgcata cgaaaaagct
    13321 tctgctccaa atgcagcact acctacttct accccagata aattggatgt aagagatatt
    13381 actccaagct ctgttctagc tgctccgaca tcacagagcc tcatccaagt cgtaggagcg
    13441 ccaatcacag ctaccagtga taataatgta ataacaggag atagagaagg tccaatggct
    13501 gtaacactag ctgagcgaat tgctgcttta attcctttct tttcaatacc aagatttgaa
    13561 gcctctttta gtgcctcttt cataaatacg attgcttgaa caactgaaat aattactaaa
    13621 aaactagaaa caatccaaag acctgggcta ttaatcattt cttgaaactt catactttat
    13681 tcctccttaa aataaatttt tcagcatttt attgtgagct ttctttctgt aatcttgtta
    13741 tgtttttata ttgttttaat ttattttatc taagatatag tatctttaat aggagattat
    13801 ctgtcttaaa gtctcactaa aaacattaat acttaaatat tttattcata attagtaaat
    13861 atcagaaaat tcacatatta tttttttaac ctgtaa
//
LOCUS       DAMJZE010000103        13882 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJZE010000103 DAMJZE010000000
VERSION     DAMJZE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.104"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            154..1659
                     /locus_tag="PC541_002517"
     CDS             154..1659
                     /locus_tag="PC541_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2839973.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2049..3146
                     /gene="dinB"
                     /locus_tag="PC541_002518"
     CDS             2049..3146
                     /gene="dinB"
                     /locus_tag="PC541_002518"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2839974.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3409..4545
                     /locus_tag="PC541_002519"
     CDS             3409..4545
                     /locus_tag="PC541_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839975.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4538..6085
                     /locus_tag="PC541_002520"
     CDS             4538..6085
                     /locus_tag="PC541_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2839976.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6420..7286
                     /locus_tag="PC541_002521"
     CDS             6420..7286
                     /locus_tag="PC541_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2839977.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7427..8569
                     /locus_tag="PC541_002522"
     CDS             7427..8569
                     /locus_tag="PC541_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2839978.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            8953..10191
                     /locus_tag="PC541_002523"
     CDS             8953..10191
                     /locus_tag="PC541_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839979.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            10220..11107
                     /locus_tag="PC541_002524"
     CDS             10220..11107
                     /locus_tag="PC541_002524"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2839980.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            11141..11770
                     /locus_tag="PC541_002525"
     CDS             11141..11770
                     /locus_tag="PC541_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2839981.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            11775..12239
                     /locus_tag="PC541_002526"
     CDS             11775..12239
                     /locus_tag="PC541_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2839982.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            12489..13856
                     /locus_tag="PC541_002527"
     CDS             12489..13856
                     /locus_tag="PC541_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2839983.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
BASE COUNT         5252 a         1469 c         2384 g         4777 t
ORIGIN      
        1 taccataaaa ctatcttgaa aaaaattaat cttaattgta taatattact ttgagtgaaa
       61 aagtattaac atttttttat aagatgtttt actaaaaaaa tatatataaa tatactgtta
      121 tcaacaagaa atttaattta gtgaggtgtt aagatgaaag taggatttga ccacaaaaaa
      181 tatttagaag agcagtctaa atatatctta gaaagagtaa ataattttga caaactttat
      241 ttagagtttg gaggaaaatt gatgggagac cttcatgcaa aaagagtact tccaggattt
      301 gatgaaaatg caaagataaa agtattacaa catatgaaag aaaaagttga agttgtaatt
      361 tgtgtctatg caggagatat agaaagaaac aaaataagag gagactttgg tatcacttat
      421 gatatggaag ttttaagatt aatagatgat ttaagaggtt atgaactaga tgtaaatagt
      481 gtagtaatta ctagatttga aggacaacct gctacaactg tatttataaa taaattagaa
      541 cgtagaggaa ttaaagttta taagcattat ccaacaaagg gatatccgtc agatgttgag
      601 actatagtta gtgatgaggg atatggagca aatgaatata tagaaactac aaagccaata
      661 gtagtagtca cagcaccagg acctaatagt ggaaaacttg gtacttgtct aagtcagttg
      721 tatcatgaaa ataaaagagg taatgtagtt ggatattcta agtttgagac attcccagtt
      781 tggaatgtac ctgtaaaaca tcctttgaat atagcttatg aagcagctac agttgattta
      841 aaagatgtga atatgataga ttcattccac cttgaaacgt atggtgaaat gtcagtaaac
      901 tacaatagag atttggaatt atttccagtg cttaaaaaaa taatagaaaa gataactgga
      961 aaagaatcaa ttttccaatc tccaactgat atgggtgtta atagagttgg ctttggaata
     1021 atagatgatg aagtaataag aaaagcttct atagaagaaa taataagaag atactttaaa
     1081 actgcctgtg agtataaaaa gggacaagtt gataaaggtg cttatgatag aatgaaaatg
     1141 attatggaag agcttaactt aaagccagaa gatagaaatg ttgtattacc tgctagaaac
     1201 tacagtaata aattgaaaga aagtgccgac aagaatgata cttgtcctgt agttgcatta
     1261 gaattagaag atggtacaat attaactggt aagggttctg atttaatgaa tggaactgca
     1321 gcagtagtat taaatgccat taaacattta gccaatatat ctgatgatat gcacttaata
     1381 tcacctgtaa tattagaacc tatcataaac ttaaaaacaa agactttagc tagtagaaat
     1441 gtttcactta gttgccaaga ggtgttaact gcacttagta tttgtgctgt aactaatcca
     1501 actgcacaat ttgctatgga aaagttagca ttattaaaag ggtgtcaagc tcattctact
     1561 actatattaa atagagatga tgaacaaaca tttagaaaac ttggtataga tgtaacttgt
     1621 gacccagaat atccttcaca aaatctttac tatagttaaa attatataaa aaaatatggg
     1681 ctgttttaaa gtaatagttt gagcagtcca ttacaattat aataaaatca gtaggctgtc
     1741 ttgtataaga cagtcttttg tttttaaaac atcccataaa ttaaatatat atactgttat
     1801 ccataaggaa ttttaaattt agttccttgg aatacctatt gtaaagcttt tatatagttt
     1861 ttgcaagtgt ttttaaggat tttttataaa ttatattgaa gttaatggat tatagatggt
     1921 tttatttatg gaaaatatga atatataaag aaaataatga aaaaatacca aaaagaaaca
     1981 taagtttggt ataattaaaa agtaatataa ataaaattaa tctatttata ctttaaaagg
     2041 tgataataat gaaacattat agaaaaataa ttcatataga tatggacgct ttttatgcat
     2101 ctatagaaca aagagataat aaaaaattaa aaggaagacc tgttattgtc ggtggaaatc
     2161 cacaaagccg aggtgtggtt gcaacctgtt cttatgaagc tagaaaattt ggtatccatt
     2221 cagctatgcc atcagctgta gcttacaata ggtgtccata tgcagttttt gtaagaccta
     2281 gatttgatgt ttataagagt gtgtcagagc aaataagaga tatattttat agatacacag
     2341 atttagttga accattatct ttagatgaag cttacttaga tgtaacaaaa aataaaaaaa
     2401 atattgattc atcaattgag atagcaaagc aaataaaaaa agatatattt agagaagttg
     2461 gtcttacttc ttcagctggt gtgtcataca ataagtttct tgctaagatt gcatcagact
     2521 taagaaagcc aaatgggtta actgtaataa ccgaagaaaa tgcccaggac tttttagata
     2581 aattacctgt gaataaattt tttggtgttg gaaaagttac ttcaaatact cttaaaaatt
     2641 taggtataaa aactggatat gatttaaggt gtttaaattt atttgaactt gaaaatatat
     2701 tcaaaaaaag aggttatgaa ttatataaat ttgctagagg aatagacgat agaccagtgg
     2761 aaccaaatag ggttagaaaa tctgttgggg ctgagactac attaagtcat aatttggata
     2821 ttgatgaaga agaaacacgc aacattcttg atgaactttg tgaagaagta tgtcatagat
     2881 taaaaaactc tgagaagttt ggaaaaacat taacattaaa aattaaatat gaggacttta
     2941 caaagataac tagaagcctt agtttagaac actatattga cgaatataat gatataagaa
     3001 gtggtgtaga taatttgttg agaaatgttg aggttaatgg aaaacaaatt aggctattgg
     3061 gagttacaat atcaaaccta agtgataaaa aagaaactta taaagatatt acattatttg
     3121 aatacatgga tagtatccaa atgtgagttt tgtttaaatg aaataacact agctttaata
     3181 ctatataatt gaggctagtg ttattttatg aatattttta tattttttgt tttttgacat
     3241 tgtatattat atactatata taaatacttt atatttgtag caatattcag acaaatagtg
     3301 acctaaaaat cgtactttgt ttacataaat aaaatagtat gatataatta taaaaacaac
     3361 ttataatatt ggagaaggtt gcttgttcga aaaaggggat gataaattat gatagagata
     3421 agaaatgtaa caaaaaaaat aggaaataat gttatattag atgatatttc tctagttgta
     3481 gaaacaggta cattggtagt tttgattggt tcaagtggtt gtggaaagac tactactttg
     3541 aagcttataa acaaattaat aaaacctaca tctggtgaga tatatattaa tggcaaacca
     3601 atttctcagg aaaatgaaat agagcttaga agaaaaatag gatatgttat acagaacaca
     3661 ggcctattcc ctcatcttac aataaaagaa aatatagagc taattccacg cctaaaaaag
     3721 gaaaaatccg ttgaagaaat tgaaaaaaga acgttgcaac tacttgagat ggtaggactt
     3781 gatagtgatg aatttttgaa taagtatcct tcagaactta gtggtggaca gcaacaaaga
     3841 atcggtgttg caagagccat tgctactgat gctgaaataa tattgatgga tgagcctttt
     3901 agtgctttag acccaataac gagaacatct ttacaagaac aattattttc tcttcaagat
     3961 gaattgaaaa aaactataat atttgtaacg catgatatgg atgaggcttt aaagatagct
     4021 gataaaattt gtattatgaa ggatggaaga atagcacaat atgatactcc cgaaaatata
     4081 cttagaaagc ctgcaaatga ctttgtaaaa gattttatcg gagaagatag agtttgggat
     4141 aatccagaat atataaaagc aagagatatt atgataaaaa atcctattgc tgttaattct
     4201 actagaactg taactcaagg aatagaaatt atgagaacga gtaaggttga tagcttacta
     4261 attatagata gagcaaaaac attaaaaggt attgttactg taaaagatat gaaagatatt
     4321 gatgataagt caatattact tgctgatatt atgtcaagtg agcctttgca tgtaaatgaa
     4381 ggggataatt tagtagaaat actaaatgta atgaatcgta attcggttgg atatattccg
     4441 gttatttctg atgaaaataa actggtaggg cttattacta gaagtagttt actttctgtt
     4501 ttaagtgaac aattcttaga aatggaggtg agtgttcttg gctaattttt ttaactttat
     4561 actattacaa aaagataaga ttatagagct tttaatacaa catatgagtc taactgttac
     4621 atctatttta atagctataa tagttggtgt tcctcttgga ataattattt caagaatctc
     4681 atctcttaga aagtttgtac ttggatttgt aaacttagtc caagcagttc cgtctatggc
     4741 acttttgggt cttcttgtac ctatacttgg tataggaagt aaaccagcca tatttatggt
     4801 agtggtttat tcattactac caattgtaaa aaatacgtat ataggtataa caagtattga
     4861 cccagtagta ttagaatcag ctaaaggtat tggtcttaca agaaatcaga cattatttaa
     4921 aatacaattc cctttagcac ttcctataat aatgggtgga gttagaatat ctgcagttac
     4981 agctgttggt ttgatgactc ttgcagcatt tataggtgct ggtggtttag gatatttagt
     5041 attttcaggg gttcaaactg taaataataa tatgatttta gcaggggcaa tcccatcatg
     5101 tataattgca ctgttagttg attatttatt tagtaaaatt gaagttgcag ttactccaaa
     5161 aggattaaat ccaaaagcac ctaagaagaa ttatatagca cttaaagtaa tatctgtagt
     5221 ggttgttgtt tcaatgttgt tcgttgtttt tagttctagt ttttcaagta aaaaagatac
     5281 aataactatt ggttctaaag attatacaga acaacttata cttggtaatg tttatgctga
     5341 attagtagaa aaaaatacga atcttaaagt taagaagaat ttaaatctag gtggttcatc
     5401 tgttgcgttt aatgcaatta aatctggtga gttagacatg tatgttgact atacaggtac
     5461 acttttggta aatgtaatga aacatgctcc gattaaagat gctgatgaag catataatgt
     5521 tgtaaaagat actatggaaa aagaaaatca gttaacttta cttgaccctc ttggatttaa
     5581 taatacttac acattagcta tgatgcctga aactgctgaa aagtatggaa taaatactat
     5641 ttctgatttg actaaatatg gtaaagaatt tacattctca ccaactttgg agtttgaaaa
     5701 tagagaagat ggtcttgtag ggctttctag agattatggt ttaaaattca aagatgtaaa
     5761 agcaatgaat ggaagtttaa gatatacagc tttagataat aatgaatctc aggttataga
     5821 tgcattttta actgatggtc ttattaaaaa gtttaatctt aagatattag aggacgataa
     5881 aaatttcttt gtaccatatt atgcagcacc attagtaaga gaagatactt tgaagaaata
     5941 tcctgagtta gaaaaagtta tgaatatgct ttcaggaaaa ataaatgaag aaactatgag
     6001 agaacttaac tatcaagtgg atgaacttgg taaatcacca gaagaagttg ctcattcatt
     6061 cttagttaaa gaaggcttag tataaaaaat taatagttta tatcaaaaaa tttaagagag
     6121 tgtctcaaaa tgaacttttt agttcatgag gcactctttt ttatatgaaa tcaaatatat
     6181 tttatcattt caacaataaa aaagaagatt atctttattt tgagacagtc tcttattttt
     6241 atgtatacca aaaccatacc tacgattttt atttataatt aagtttcaaa tagaatgaac
     6301 aagtacaacc aattttgaat ataaatatag taaatgtgct tatatacgat tttattgtct
     6361 ggttgattga tattgataga tagctttctt tattgaatcg aagggagggg gaatttgtta
     6421 tggataacta taagaaagtg aagcaagtat tgtggataat tttatttgct aattttgcag
     6481 tagcattatt aaaaatcatt attggtaatc aaataaaaag ctatagtatg acagcagacg
     6541 gatttcattc tttgtcagat ggagcatcta atatagtggg gcttataggt attttctttg
     6601 catctaaacc aaaagataaa aatcatcctt atggacataa aaagtttgaa attataacca
     6661 gtttatttat aagtggtatg ttgtttgtta tagcaattaa gataattctt agtgcagtgt
     6721 taagaatagc taatccagtt gtacctgcta taactataga aagtttaatt gctttaatta
     6781 ttacactttt tataaatatt tttgtatgca tgtatgaata tagaattggg actaaactaa
     6841 atagttatgt tttgatttcg gattcattgc atacaagaag tgatatattt gtgtctctag
     6901 gtgtattggt tactttagtt ggtgttaagt taggatttcc agtaataata gaatctatag
     6961 ttcctattat tatttctgca ttcataattt attcggctta tggaatattt aggccatcta
     7021 ttggaatttt ggtcgataga gtggctgtag atgaggatta tataaaagaa attgtgtttg
     7081 aatttaatga ggtaagggat gttcacaata tcagaagtag aggtagcaaa agttctattt
     7141 atattgacat gcatgtaatg gtagacccat ttataagcgt agagcaatct catgatttaa
     7201 cacataaaat tgagaaacaa atacaagaag aaattaatga aaatgctcaa gtaatagtcc
     7261 atattgaacc tttttatagt ttttaactta gctataaaat acaagttata aaaaaatata
     7321 atttataaaa aaaatattat accataaata atttataaaa attatttatg gtataatatt
     7381 ttctataaag aaattaagtc ttaatatatc taaggaggaa tttacaatga gatacgttga
     7441 atatggaaag acagggaaaa tggtttcagt agttggctat ggaggattaa ggtttgattt
     7501 agaaaaaagt aatcaagaaa atgctgattt agtcaaatat gcgtatgaaa aaggtattaa
     7561 ttactttgat acagcaccag ggtattgtga tgacagaagt gaagatatat ttggactagc
     7621 ctttagacaa atgataaagg aaggtaagac cgatttttat gtatcaacta aagaaaagcc
     7681 aaaagcatgt gatacatcag aaaaagctat agaggctgta aaaaaatcaa tagaacgatt
     7741 aggcgtatct aaaataaatt tttatcatgt ttggaatatt agaaagatgg aacattacga
     7801 actagcaatg agacctggag gtcaatatga aggattattg aaatgtaaag aagaaggatt
     7861 gatagaccat atagtatttt catctcatca accaggagat gaggttatag atattctaag
     7921 tgaaaataag tttgaaggtg taactatggg aataaatata ttaaactttc catataggct
     7981 aaaaggtgcc aaatatgctg ttgataatgg atatggtgtt gtggcaatga atccattaag
     8041 tggtggaact attccaaagt ataataaaga actttctttt ctatcaaaag atggcgaaag
     8101 tactatagaa tcagctttaa gattcaacat aggaatttca caaataagta tatctttgat
     8161 agggtttaat aaaaaacaag atatagatga tgcatgtaaa attgcagacg aaaatagaat
     8221 ttattcagat gaggatatac atgatattga gactagatta aataagaata tgaacgaaat
     8281 atgtacaggt tgtggttatt gtaaagtatg tccaaaaggt attaataccc cagcctatat
     8341 gcttttttat aatgaaaagc agatgtttaa aaagagtgat gaagaaatga caaagttagt
     8401 ttatggctta ggacattgga attacacaat gaatagtaag gcaaaagcta aagaatgtat
     8461 atcttgtgga aaatgtgaag ttgaatgtac tcagcattta cctataatag atagattaaa
     8521 agaaattaaa aaatgggaag aagatggagc taatagtgtt aaagtctaat taattttata
     8581 ctatatttga aatttgtata gtatttagta atgttttaaa atatagtttt aatatttaag
     8641 gagacagatt ataattttgt ctccttaaat tatgctttaa tttgttatag tttaattcca
     8701 taagtaacat tgtatatttt tatataaagt ttttcaaata ttttacatta atattgctta
     8761 caaattctca ttttatacaa cactaatcta tgttatataa acaatacttg actgattata
     8821 acatcaataa agtaaatatt gtggttgtaa ataaagagtt attcaattac aataaaacaa
     8881 gtaagaacat tatgaagatg gctattaaat ataaggaata aattgtaggt gtcagcaaat
     8941 cctatataat gaatggatgg tgatatattg atgagtaaaa tattaaaata taaaaacgag
     9001 atgtttttgt ttatattagt tgtaataact tacttaataa taactaagat atttttttct
     9061 aagactacta tattttatga tttaaacaat acttatgatg tattgttaga tacagataca
     9121 ggagttttat tcaatttaaa tgtatttgct ataagtcaag acaatagtaa gcatatattg
     9181 ttttcagcaa ttatatcaat ttttgcatat ccaatatacc tattttgtac ttcaatagca
     9241 aatcctggga caacagattt taatagtgcc tatgggtttg gactaatttg tctacaaata
     9301 ataactagtg ctatgtcaat aactttagtc tttaatcata ttaaaaagat aaaaatgcag
     9361 agattaactt taattttact tactatgata atgatatttt cttttccaca gttatttatg
     9421 actttaaatg tagagagatt tatatatagc caattttcat taatattttt catagttatt
     9481 gcaaataaga tgaaaggaaa aaattcttat ttggtagaat tagcagctat tcctctattt
     9541 ggaataacta ttagtaatat atatttgtat ttttttaata tgatatttga gtttaaatta
     9601 aaaataggaa aaatgttaaa acatttaatt acatttatcc taatggcata tatttgtgta
     9661 gtttcaacga agtcttatga gagttttatg aaccttggaa atgtaataca gtatgatacg
     9721 aaatttataa gtggagaacc aattttggaa aagattgcaa tgattataga gagattattg
     9781 tattcggttt tttattttcc tggagcaagt attaaaaaag ggttattttt gcaaaatgga
     9841 gaagttgcta caataccagt aattcttaca ttattagctt tatgtttttg tgttttatca
     9901 gttattgaaa attcagaaaa aagagttcct aagctatgta tgggaataat tatattcaat
     9961 ctaactttac atggtatagt aggatataat ctagtcaatt caagtattat ggctataaat
    10021 tttagttttg cagttataat tttattggct tactttacga aagcccttag aaaaaatgaa
    10081 aagaatatgt ataatatatt tctatcgctg ttattggtta ctatagtaat cagtaatgtc
    10141 aatggattta tagaaatttt aaacataggg ataaaatcat atcctgttta aaaaataata
    10201 ttgaaagggt agtaagttta tgaaacagac caatacaagt ataaggcttc ttactaaaat
    10261 ttcagattat ataaagttaa tgagaataaa gcaatatata aagaatttct ttgtattttc
    10321 agctataatt ttttcaaaca acatactaaa tataaattta tttttaaata cgtttattgc
    10381 gtttgtgtgt ttttgcctaa tgtcatcatc agtatatgca ctgaatgatg ctatagacat
    10441 ggataaagat aaaaaacatc ctaaaaaatg taatagacca gtggcaagtg gaagaataag
    10501 caaaaaaagt gctaatatac tatttgtagt attagcgtta atttcagtct gtttatcaac
    10561 agttgttagt tttaatcttt ctataattct cagtctctat ttgataaaca atatattata
    10621 ttctcttaaa ctaaagaata taatactatt agatgtgttt tcaatatctc ttggttttat
    10681 acttagggtt tatgcaggat gtgtagccat aagtgttagt ttatcaaatt ggataatttt
    10741 atgtacattt ttcttatctc tgtacttagc tcttggaaaa cgtaaaaaag aaatagaaac
    10801 tctaagagat gatgcagttg aacatagaaa aatattggaa gattatgaca tagaaaatct
    10861 aaatcaaatg atgatagtta tattaagttc tacaattgta tgttatgcat tgtattctac
    10921 aagtaatcca gaaaagccac atatgatatt tacaacaata tttgtagttt atggtgtact
    10981 tagatataat tatataatta acactacaaa tgaaaataat cctactgata ttgtcctaaa
    11041 agataatgct ttaaaaataa acgtaatctt atggataatt acatgtttaa taatattgat
    11101 tttttagtga tatttttagt gataggatgg ggaaatactt atgaaaaaaa gtgtattatt
    11161 acttgatgta gattatactg ttataaatac tgatagcatg atagattttt ttatttactc
    11221 tttaaaaaat aaaaccttta aaactattat aaagttgcca tacattattt ttattctatt
    11281 tatgtattta attagaatga tacccttgaa gaaggcaaaa gaggcaatct tttatccaat
    11341 agtagatttt agtgaagaag atttaaaaaa gttttttgat gattgcataa tgaaaaaaat
    11401 taacgaatct atgaaaaagg ttatttacaa aaataaagaa gaagataatg ttataattat
    11461 gataactgca tctccatatg catatatgaa atattttaag tattatggtc ttgcagacga
    11521 ggttataggc acggagtttt tttatgaaaa ttcaagatat aaaaatcaat tcattggaga
    11581 aaactgtaag ggaattgaaa aagtaaaaag aataaaggct gtattaggta aacttgggat
    11641 agagattgat tatgaaaatt catatgcata ttcagactct aagagtgatt taccaatgct
    11701 ttcattgact aaaaatgcat ttttagttag taaaaaggat ggtgaggtta tagagcaaat
    11761 aaattcataa cttaatgtca cttagaatag gagggaatag tttgggatat ttacacatat
    11821 taattttatc tatggttcct gtaacagaat tgaggggagc cattcctata ggtattgcaa
    11881 tgggcttaaa tcctatatgg gtttatgtat ttagtgtaat aggttctaca ttggtatcga
    11941 ttcccttgat attgacattc agacatgtat tacaattttt aagagggaag aaactattta
    12001 aagggattgc tgatgtaatt gatagaaaaa taaactcaag aatgaagaaa ttaaaaagtg
    12061 tttcgataat tggtataata ctatttgtag gtataccact gccaactaca ggaacatgga
    12121 ctgcatctgc tatagcttcc atattaaaaa tgagaataaa agatgctttt atgggagtat
    12181 ttcttggcaa tttgttatca ggggttatag tttcagctct ttctttacat ataatataaa
    12241 actatctaag taatatactt ggatagtttt tttatgagta tatgtgaaaa atttttaata
    12301 ataaataaaa aatgagataa agtatttaac ttcagttagt tctaaaatgt ttactatggc
    12361 aaaagaaata tagtatagta ttacttgtag ggaaagtact ctaaatttgt ctgctaataa
    12421 aatgaaattt ttatatagaa taaaatttta gtgatacatt ttaattattg gattgaggag
    12481 gttttaaaat ggaaaattca aacactaact taggtagtga aagtgtgggt aaattattat
    12541 ttaaacttgc tacacctgct attatcgcac aaattgtaaa tgttttatac aacatagtcg
    12601 ataggatttt tattggtaga atggaaaatg gagaagttgc tatggctggt gttggagtgg
    12661 catttccaat aataattata ataactgcat gtagttattt aatcggtatg ggaggaggtc
    12721 cattagctgc tataaaaatg ggtgagcaaa acaatgatga ggcagagaaa ataatgagta
    12781 acagtttttc tgtattggtg attttagcaa tacttttgac tataggtttt aagattggaa
    12841 aagagccttt actttggatg tttggagcaa gtgaatctac aattggatat tcaatggact
    12901 atttaaatat ttatttaata ggtacagtgt ttgttcagat ttcaatgggt atgaatactt
    12961 ttataaatac ccaagggttt gctacaactg gaatgatgac agttgctata ggtgctttaa
    13021 taaacataat acttgacccg atttttatat ttggctttaa tatgggtgta aaaggagctg
    13081 cattagctac tataattgca caaggcgttt ctgctatttg ggtacttatg tttctctttg
    13141 gaaagaaaag tatacttaaa ataaaaaaga agtatatgat accaaaagca agtatcatct
    13201 taccagtgct tggcttggga atctctccat ttataatgca atctacagaa agtttagtac
    13261 ttatagcctt aaatagtaaa ttacaaatgt atggaggaga tttagcagta ggttcaatgg
    13321 ccataatgag cagtataatg caaattctta tgcttcctaa tatgggggtt acacaaggag
    13381 cacaaccaat aataagttat aattatggtt caggtcaact tgatagagta aaaaagacat
    13441 ttaagttgtg tttattgtca tgttttacat attctactat tttatggctt ttattaatga
    13501 tatttcctgc attttttgtt agtatattta ataagaatcc gcagttactt agtatgacta
    13561 gttggagtat aaaaatatat tttgctggtg cttttatgtt tgggattcaa atagcttgtc
    13621 aacagacatt tttagcactt ggaaaagcaa caatatcttt ggttttagct ctacttagaa
    13681 agattgtact tttaatacct ttaatattta tacttccaac attctttaat gaaaaacttt
    13741 ttgcagtaat tttagcagag ccagttgcag atataacagc cgctactata acagcaattt
    13801 ctttctttat attttataaa tgttttctat caaaacctaa agcaataaaa gaataagata
    13861 tacaatataa attttttatg aa
//
LOCUS       DAMJZE010000104        13777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJZE010000104 DAMJZE010000000
VERSION     DAMJZE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.105"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            263..493
                     /locus_tag="PC541_002528"
     CDS             263..493
                     /locus_tag="PC541_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2839984.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            550..1200
                     /locus_tag="PC541_002529"
     CDS             550..1200
                     /locus_tag="PC541_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2839985.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1414..2085
                     /locus_tag="PC541_002530"
     CDS             1414..2085
                     /locus_tag="PC541_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2839986.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            2082..3212
                     /locus_tag="PC541_002531"
     CDS             2082..3212
                     /locus_tag="PC541_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2839987.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3632..4477
                     /locus_tag="PC541_002532"
     CDS             3632..4477
                     /locus_tag="PC541_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2839988.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4477..5505
                     /locus_tag="PC541_002533"
     CDS             4477..5505
                     /locus_tag="PC541_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839989.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKTKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5508..6521
                     /locus_tag="PC541_002534"
     CDS             5508..6521
                     /locus_tag="PC541_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2839990.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(7038..8288)
                     /gene="cme"
                     /locus_tag="PC541_002535"
     CDS             complement(7038..8288)
                     /gene="cme"
                     /locus_tag="PC541_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2839991.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8374..9120)
                     /locus_tag="PC541_002536"
     CDS             complement(8374..9120)
                     /locus_tag="PC541_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2839992.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9235..10257)
                     /locus_tag="PC541_002537"
     CDS             complement(9235..10257)
                     /locus_tag="PC541_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2839993.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10257..12677)
                     /locus_tag="PC541_002538"
     CDS             complement(10257..12677)
                     /locus_tag="PC541_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2839994.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12763..13683)
                     /locus_tag="PC541_002539"
     CDS             complement(12763..13683)
                     /locus_tag="PC541_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2839995.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4856 a         1795 c         2174 g         4952 t
ORIGIN      
        1 aataagctat aaaaactcaa tattgttttg ttcaaagata aaaaaagaat actttaggtg
       61 atttttatta aaaattaaga gtgataagat tatatacttt ataaatgtaa atatgacgga
      121 taaaaatgat aaatataata atatatatat ttacattagg agttttctat actataatta
      181 tttaaaacta aatataaatg ttggaattat ttaggacttg aaattgtcta aaaataaata
      241 taagtgatga agaggtgatg tgatgagatt agatatggat agtatgttat atgtactaat
      301 ttatatgaca ttattggtat ctatcattgg aagtatattt tatttttgta aagacctctt
      361 aaatcataaa attaagtatg ttttaataag agacatattt gtaaatagaa ttacatattc
      421 tgaagaacgg ttgcctaaac agtatatagt ttttcagaaa tatgatattt ggaggtattg
      481 tagtttgttt taatttgaaa tgaatctatt ataaattcta aagtttaaaa ataaaaagga
      541 gagaataaaa tggatttcat ttcaagtata ttgcaaggat tattaagtat attatttaat
      601 tttacaggtg attttggaat ttctattgta cttataactt taatagttaa attagtactt
      661 atgccactat cattaaaaca aagattttct gtaaaaaaac agcaagaatt agctgaaaaa
      721 atggaacata taaaagaaaa gtataagaat gatactaaag aattggaaaa gcaaatacaa
      781 gtaagttctg tagaaagtat gaagagtatg ctgggatgtc ttatagtttt ggttcaaatg
      841 ccagtagtat atgctttata tcatgtatgt ttagatatga caaaggaatt tacaacaatt
      901 ataattccat ggattgctaa tttaggtaca tttgataatt tgtttatatt accttgtata
      961 tatacattaa ttatgttagc ttcaaattta gtgaattata taccatattt aaaaataaat
     1021 tctcaagtaa agtttaacaa acaaatggct gtggtgacta ccataacaag tcttatacta
     1081 actgttagaa ctccagtagc cataggtctg tactttataa caagtgcaat ttattcatta
     1141 attgaagata tttgttttag aatatatttt aatcgaaaaa atagaatagt gacaaaataa
     1201 tataattatt attagaaata ttttagcctg ctaacataga aaaagttagt aggctatttt
     1261 tatctatgat atcaattcca ttaagaatag tctaaaaaat actatcaaaa tgctataaaa
     1321 aatactatca aaatactata aaaaaatagt tgaaaaataa ataaattaat ataaactaaa
     1381 ataaagccta caaaaaagag gtgataaata gaaatggcta aaatactaat tgtagaagat
     1441 gaaaaaagga tgcaaagtat tattgtagaa tatatgcaaa agggaggata tacttgtata
     1501 actgctgatg atggagtcga ggcacttaca atattaaaga gcaacaatat agatttaatg
     1561 atattggata ttatgatgcc gtatttagat ggattttcag tgtgtagagt ttcacgtgaa
     1621 atgactaata tacctattat tatattgaca gctaaaggtg aagaggagga caaattaaag
     1681 ggttatgaat atggcgcaga tgattatata acaaaaccat ttagccccaa agtactttta
     1741 gcaaaagtta atgccttgtt acgtagatac actactgata ttcccaaaaa ttcattgagt
     1801 ttaggaaaga tttttattat ggtagcatca aggcaagtgt atgtagaaga taaattgatt
     1861 gatttaacat ataaagaatt tgaattattg cgtttattta tggaaaaccc taatcaagtt
     1921 ttttccagag agaaactttt aaattgtata tggggatatg attttgaagg aaacacacga
     1981 actgtagata cacatataaa aaccttacgc aaaaagcttg gtagtgaagg acaccatata
     2041 gttacattaa ttcgttcagg atataagttt gaggttaagg aatgaatagt ttaagaaaga
     2101 ttatagaaaa actgaaaaat gtagatattt caaattggat taaacaaaat tttggactat
     2161 attcagttag aaaaaaagtg tttttacttt ctaaattatc aggagcgtta attatattat
     2221 tttatctggt tgttgaagaa ttttcgataa actctaaatt agaattttgg atttggttta
     2281 taatactggt tatatttatt atgagtattg atttcttatt gggaaaattt atttcagaac
     2341 caatcacatc tataaataaa tctgcaaaaa gtatgtccca acttgatttt tctaatccat
     2401 gtactgtaaa tactaatgat gaatttggag aactttctag gagcctgaat acaatgtcta
     2461 caaatctaca acaggcatta tcagacctag aaagtgcaaa tattcaactt gaaaaggatg
     2521 taaataagga aagaatgcta ttggaacaaa gaaaggagtt ggtagataca atttcacatg
     2581 aaatgaagac tccacttggc attatacgtg cgtatacaga ggggcttatg gatgaagttg
     2641 atgaagaaaa aaagaaaaat tatatgaatg ttataataga agaaactgat agaatgaaca
     2701 atatgattgt atcattatta gacttatctg cattggaagc aggagtttct aaacttaacc
     2761 cagagcgttt tgattttata gagtttgttg aaactgtggc aggtcgtctt ttaattgata
     2821 taccagatgc caatttttat tttacatatg atttgccaga gtatgaagtt tttgtagtgg
     2881 cagataaaat gagaatggaa caagtagtag agaatctagt tattaatgca aagaagcatg
     2941 ttgtatataa tggtaattta gatttatcga ttacatgtga aaatggcttg cttttatttg
     3001 aaatttataa tgatggtagg tttatagagc aagatgagat ttcaaagatt tggtctaagt
     3061 tttatcgtag tgttcaatct cagcgtacag gaggttcagg tctagggctt gcaattgttt
     3121 cacaaatact tacaatgcaa gggttagaat atggagtgaa taatcgtgat agaggagtag
     3181 agttttattt tatgattcca attgatgaat aatttagtat taatttataa attataagta
     3241 tctgatgtaa aatatttttc tttgtatatg taagcattta aagagaataa aataaaaaaa
     3301 cttaatgaaa aaagcttaat gaaaaagtct aataaaaagc ttaatgaaaa gtcttgtttt
     3361 tctgatgaaa acaagatttt ttattaagct ttttattatt tcacttcgat ttcacaagag
     3421 agacactctg atttcaccaa atagaaataa aataggttaa ataaattaaa aaatagaagc
     3481 gtaattaaat aaggttacaa gggggagtac ttatgaaatg gtatacatgg ttgactatta
     3541 tattgataat attgctttct attccgctta aaattaaatt tataaaatgg tgggatagac
     3601 gacaaaaaaa tgataaagga ggttatgata gatgataaaa gtaggagagt taaaattttc
     3661 ttatggaaaa gataaacaga tattgcatgg acttaatttt gaagtaaaag aaggagaaat
     3721 ctttggattc cttggaccta atggttcagg aaaatccaca acacagaaaa tcttaaatgg
     3781 tgttttaaaa ggttacaatg gtcacgtatc cctttttggt aaggaagtta aagcatacac
     3841 agaatcttta tatcaaaaga ttggtgtttt atttgaattt ccttatttat ataccaattt
     3901 aagtgcaatt gataatttag aatatttttc gtctttttat cctaaaaatc aaaggagaga
     3961 tatatatgaa ctcttagatt tgttagagtt taagaaagag tttataaata agcctgtatc
     4021 atcctactca aaaggtatga agcaacgtat tagtatggca agagcattgg taagtaatcc
     4081 aaagttgtta tttttagatg agcctactag tggacttgac ccatctggag ctgtactatt
     4141 tcgtaagatt attgaagaag aacgaaaaaa aggaacaact atctttttga ctactcataa
     4201 tatgctagat gcagatttaa tgtgtaacaa tgtggctttt attgcagatg gaaagattat
     4261 ggtaatagat aaacccaaaa atttaaaaat gaaaaatagc aataataaag ttgaagtgga
     4321 atttgtatat aatggaaatc gagatattga aattgtagat atacaagagt tagaatctgg
     4381 aataactttt aaatatgatg aaatcttaag tgtacattca aaagaaccta cactagaaga
     4441 agtttttatt aaatacacag gaaggatgtt agtgtaatgt actctaaatt ttttacctta
     4501 gtaaaaaaag atttttggat gatgatttat ggtaaatttt ttgtgctgac acttggctct
     4561 ctcatacttt attcgtgtta tattaatttt ggttatgtaa agtttatgga tgaagatagt
     4621 tatcacaata atgtatatct atataatcca gagaatataa gcatgagtgc atctccatta
     4681 attaatcaag tttcttctat aggtgaatta aaagcaaaac ttgatgaaga tacaagtgca
     4741 gtttgtattg atatgagttc cagtaagacg aaagtaattt tacatgaaag tattaaagga
     4801 gttgacgatt atagagctga ctatgcattg tcattattat catcagaaaa agaccacaat
     4861 agcaaaatat cagacaacaa tgcaataatt caaattaatg ataaggatac atctgagata
     4921 aaacaaagaa aagacatgac ttgtgagatt ttattctttg aaattgtagc tattggattt
     4981 ttagggattg cctctttatt atttaaagaa aagcagatgg gagtaatgcg tattactggt
     5041 attttacctg taaaaagaag tctttttata ttatcaaaga tatttgcttt tttgatatgt
     5101 gacctatgtt ttacagttct tttagtagta tttaatattg gattttttga aggaataaaa
     5161 atacttccac aagtattagt tcaagctagt attttatcaa ttattatgtc tttgattgga
     5221 ataggatgct caatgttatt gagagatttt aagcaatttt ctcttatata tcttgtggtt
     5281 gctgtattca taactacacc tatatttcta tctgcaaata catcttttga aatagattgg
     5341 attaaatatc atcccttcta tcatgtttat agtcagctta aaaatgcgtt tttcatgaat
     5401 ttttcaaaag aattagttta ttttgtagtg tgttctttga caataatagt tctattttta
     5461 cttgtaaaat atatttttaa taaagagttg gttagggagg gataatcttg ataggattac
     5521 gttaccagtt aaagagtata aaaaaagata aaatgtgtat aatttccttc tttttaccag
     5581 ttgttatggc gattttaata aattttatgg gaacaattga tatatcttca ttaggagaga
     5641 tacgttttgg tatagtgaag gatacacttc ctgtacaaac agagcagtgg ttgaagaagt
     5701 atggtatggt aagtgtatat gacacaaaaa tgcatttaat acgtgaaatt aaggatatta
     5761 aaacagacac aattggagtt gttagagatg gtaaagggat aaaaactgtg ctttatggaa
     5821 atgagattaa tgctataaaa aatgttggaa agacattgcc agagatgttt agactaaagg
     5881 gtgatttaaa agatgttgag gttacaatat tgcctaaaga tagtatttta aaagagtttc
     5941 aaaatgtttt tattgccatg attttaatta ctgctatgtt tatgggatgc acctttaatg
     6001 caatgaatat tatatctgaa aaagaagatg gaatatcatt tataaatgaa gttttgccac
     6061 agagtagaag tcagtatatt atacaaaaag tatttttggg tcttttatgt gggtgtttat
     6121 cttcaatgat aactgtattt atatgtttgg atatttcaat attagatatg ataatgatgt
     6181 tgttactcgt tgtattttca gcatttattt catcacttat aggtttattt attggaaagt
     6241 tgtcagaagg tcttatggta ggaattgttt atattaaaat agtaatgatt ttatttatag
     6301 gtactccttt gttaatatat cttctagggg ttaataccag tggtataggg aatttatact
     6361 atataatgcc ttcagttgct acatttaatg gtattatgga gattattaat ggtaatgtca
     6421 ttattatcaa agatattgta attttggctg tacattgtgt agtatggttt atgatatata
     6481 ttattttaga taaaaaaaga aagttatttt ttagtagata actcagtata attataattg
     6541 ggacaattta ataggtgatt taatatactt tgtatagtgt aatttatgta gtgcaatttg
     6601 tatactttaa tttatgtagt gtaatagtta gttttatttt ataattattc aatataattt
     6661 agtaagaagt tcagtacaat tagagctact tagtggaaaa cttaataggt acttaaacta
     6721 tataaatact ttattttcct ccatttgtac tataaattta atttatgata taaatggagg
     6781 tattattata tgatttgata aatttcatca ttataaccta caagtaaagg tattattata
     6841 taaagttaat aaagatagta tcaaaatatt atttgctata aagagagggg ctcaaaataa
     6901 agataaacct atttttcaat gttgaaatga taaaatatat ttgatttcat ataaaaaatt
     6961 ttcttcataa attaaaaagt ccattttaag ccccctcatt tttaaccaga taaatcgagt
     7021 taaactattt aaatatatta ctcggattca tttgacttag atattccttg cttgtaaaat
     7081 tgttttgaat aggggatagc caaagcaaaa aataaaagta taatccctgc tatgattatt
     7141 aaattattaa tagaaattac atctgccatt ggtccgaaaa tcatcatacc aagaggaata
     7201 aaaccagtat atataataat aggtaagcta agaactcgac ctagcatttg tggtgctaca
     7261 tgctcttgca acatagtcat agctgatgct tgaatgattg gaattgaaaa attcgtaaaa
     7321 aacataaata taaaaaattg ccaagcttgt gttacaagac ctagaataat tgaaaaaata
     7381 gcataggata atattgctag aaaaaaggtc ttatttctat ttttaaaacc accaaaaact
     7441 cctataatca atccacctaa aaccatacct aaaaatccaa tcatttcaat aatagacagg
     7501 tatatataac ccttgccaaa tgttcgttca ataagaagtc ctataagaaa tcctgatgga
     7561 acacaaagaa gcatgaatgc tccataagta aaaagcactt ttctaatgaa cttatctgaa
     7621 aaacagtact ttatccctga taccatatct gcaaaaaatg agactttttc agtttgtttt
     7681 ggtatatcat gtttaggaat tttgatgaat atcaataata caatagcaat aattgcagta
     7741 aatacatcaa ttaaaagaac ttgatttatt ttattaccaa atccaagtat tgcagcagct
     7801 acaataggaa ctataaattg tactatagat tgtaggctac tatttatacc attaaaacgc
     7861 attaaatgtt cttctggtac aagttgagga atcattgcgt ttacagcagg tgtttgtaca
     7921 ccacttccta gtgaacgtat aacagatatt actgatatgg ctattaattc acttgttgtg
     7981 ccatttgatg acatcataaa tagaattaga actaatgtag atatagcaat tgttgaatct
     8041 gatacaatta tgagtacctt tcgattgtag tagtctgccc atacaccagc aaaaaaagaa
     8101 attatcattt gaggtataaa ggagcataca gttaaaactg ttacccatac acctgatgat
     8161 gtctgaagtg tgacatacca tatcattgcc atttgtacaa gtgatgagcc aaataaggaa
     8221 atatattgac ttgctaaaaa aagaatgact tttttcttcc atgtagaatt agtatttttg
     8281 ttttccatcc tattcctcct ttgatttgtg tgttgaattt ttcttgctct taaggataac
     8341 acacaaatca aagtctggag ttagagtgga gggctaggaa aattgttttt tcataatttg
     8401 aagatgagat ttcatggttt ttgcattatg ttcagcttct ttcaatgaag ttatcagttt
     8461 gtcacacact gagataattt cgtcatcatc ttttggagtg tcaaggacag ttttaatatt
     8521 agtattagga ttagaagata aagcatttaa cattcgtaaa atagctgaaa gagaatagtt
     8581 tgcacaacga agtgcgctta taattttaag tcgttttata tcttcatcag tatatacacg
     8641 ataaccattt tgttttcttt ttacttttaa caaaccattc atttcccaat ttcttaaagt
     8701 atctattgta acgtttaagt gttctgcagt ttcttttcta gttaaaaaga gtttatttgt
     8761 ttctggagta tttccattta aaatctgtgc aacaatagta agtgcttcct cggcattaga
     8821 tttttctatt tctaattgtt gcaagtattg atttacaaga ctaatagcct tatcaaagtc
     8881 cctattcgct gatgttctta caatctcaag ggcatctttt cttaatcccc tttgtaccac
     8941 ttcaattttc aaggctgtac gagcaaatct aaattgttct atatgaaaat cattaaagat
     9001 acgataacca ttggtattac gcttaggttt aggtattaag tttagttctt catataaacg
     9061 tactgtatta ggatgtacac caattaaatc tgctacctct gatgtcgtgt aagtgtccat
     9121 tagtattcaa ctccttttag atatatagta ttagtcataa taacaccaaa caaaagtctg
     9181 gtgttataat ggagggtttg gttatctaag agtaattaat ttatgaaaga gtaattaatc
     9241 tattatagaa gtaagtatat atttttcaat aaactcccat ctatcacgag gtttaaacac
     9301 tattttagat ggtatggcta ttactaagtc atattgtgga atacaacaaa tagcatttcc
     9361 tccagctccc attgctgaat accacaaaac accatctttt ttacatagcc accacagata
     9421 accgtattca ttactattca ttttaactga ttcttcaatc cacgatttag atataatacg
     9481 tttattattc caaataccat catttaaata cagaaaacca aatcgcaaca tatctctagc
     9541 actcaatgta agtccccagc cacctataga ataaccctgt ctatcttttg cccaaccctt
     9601 aagatttttt ccaaagaaat cttcaaaacc aaaatctttc atatcataat ctggtataac
     9661 tttcatacca ataggtttaa ataagtattc attagcaaat tcacgagcag attttccagt
     9721 agtttttgtt ataatagctg aaagtaggtg cgttccagct gaagaatatt tgaaagtacc
     9781 aatttcgcct ttttgaccaa gttgattcaa ggtatatttt atccaatctt tttgtttgca
     9841 aagtctttct aaagactcag tcatattttt aaaagggtat ggtgcagtca ttgtaagtag
     9901 gtgacgtata gttactgctt ttttacatat ttcagaagga ctataagtat attcagggaa
     9961 aaaatcaaca accttttggt caacatcttt tatatatcct ttatctattg caatacctat
    10021 taaggctgat ataatacttt ttgtaactga tgcaacatga aaagtatcat cagcattata
    10081 tccattatag tacttttcaa agacaacttt tccttttctt gcaataatta ttccatttat
    10141 attactatat tgtgagatta ttaaaggttc aagttgtgag agtttttctg aactcatgcc
    10201 aacacttgaa ggatttactt tttcaaatat taaatctaag caattgtttt tattcattaa
    10261 agatacctcc ctgttataga ttttggattt tcttttagag tttttggaga accttctgca
    10321 attatataac ctcctttgtc accaccttct ggaccaagct caacaatcca gtcacaaaca
    10381 tttaatacat ctatatcatg ttcaacaaca attacagaat ttccattttc aaccaattca
    10441 tctaataatt gaatcaactt agaagtatcg tatagactta gaccagtagt tggctcatcc
    10501 aatacataaa ggatattacc atttcgtttt ttaccaattt cttttgcaag tttgattctt
    10561 tgggattcac caccacttaa agttggtgta ggttggccta attttatata ccccatacct
    10621 atttgttcta atacatttaa gtgtgaaaca atatttggaa tatccttgaa gaaatgtaca
    10681 gattcagaaa cactcatatt taaaatatca tgtatatttt tatttttata tttaatagat
    10741 aaagattcat cattaaatct ttttccctga cattcactgc atatatggtc tatacttaga
    10801 ttatttccca accagacctt ttcatatcca ctaccactac atttaggaca agcccctttg
    10861 gagttaaatg agaaatgacc agctgtaaat cctcgttcta acgattctgg ctgacctgca
    10921 aatagtgtac gtatcttatc ccatatacca atataagaag caggagttga gttcatattt
    10981 ttgccaatag gagattgaga tatctctgaa tatccagaaa tatgtttagt accaatcagt
    11041 ctatctgcaa tagtttcaac aactgcataa tcgtcttcct cacctatttc actctctgaa
    11101 gaatttattt gattattgat agtatggtta ttaaattggc ttctaagaag aggaagtagg
    11161 gtgtctgata taagtgaact cttgccacta cctgattttc cagatattcc aaccatagca
    11221 cccaaaggta atgatacagt tacattttta agattatttg tatttgcatt ttgaatagtt
    11281 atacaaggta ttttatcagt atgtgtaatg tttttattgc ttgttctatt aggcattgga
    11341 tattttccag atagatactg tccagtgagt gatttgtcgg attgaagtag accttctaaa
    11401 tctccttgat atactaattg accaccttca acaccagctt ttggaccaat atcaataata
    11461 tgttctgcca ttttaattgt gtttttatca tgctcaacta ctatgaccgt attgcctaaa
    11521 tctttaagtt tctttattga ttttaagatg tctatttttt ctgattcatg caatccagct
    11581 gttggttcat caagcacata aattaatgag tccatctctg aatcgagatg tgaatttaaa
    11641 aatagtcttt gcatttcccc accactcaaa gttgacattt ctcggtataa ggatagatga
    11701 cctaatcgtg attctattaa atgttgagtt ttggataaaa tatcatttac aagatttgta
    11761 ccaaatgtgt ttaaattacc ttgatttatt aagagtttaa ggaaatcatc aatttctaaa
    11821 atcgtcattt ttccaagttc tccaatatgt tttccgttta aaagtactct acgtgcttct
    11881 tcaccaattt taaaaccatt gcaatctgaa caggcagtca tagtataaac tccatttaaa
    11941 tcttcgcccc ttttgtaacg attgtgaaat acctttgata aagaggaact ttttttacca
    12001 ttttcataat gaccatagag tacttcattt cttacttctt caggtagttg taagaattgt
    12061 atgtctaaat aatcattgaa ttttttagaa aatactctca aatatccagg tgtaatcttt
    12121 gctctatcta agacttgttt tagggtatca tgcttatcaa ggactaattt ttcaatatta
    12181 atctcaaaat atgcaccatg tccagagcac ttcatacaca ttccatctga agagttgtat
    12241 gagaaataac ttggtcttaa tcgttcttcc tcatatccac aattattgca aattagatta
    12301 tagcttacag gagtatcaca catagtacac attatttgac cttccactga atacaatagt
    12361 gtaagcatgt tgagaatatt ggttcttgtg cctactgttg aacgaggatt gctctggcga
    12421 ataatatttt gttgaacagc tatagtggga gctaaacctt gaatattatc aaacttatct
    12481 tcactatcaa tacctgcaag tatacctaaa gattgcagat attgtttacg cccttcctca
    12541 aatacaatgt caaacataag gctagactta cctgagccac tgacacctgt tgcaacaaca
    12601 agcttatctt tggggataga aacatctata tttttaagat tgtgtatctt tgctccatta
    12661 atttttattt ctttcattgg ttcacctcat tttatcaatt ttatcaaacc gtaaaatagg
    12721 gatatattaa tccttaatac aagaatagat taggtgaggt tattaaaaag gttctatagg
    12781 taaatatata tcgaccaagt gtctattttg agggtgattg tgtgggttgt taagatatac
    12841 ttcaaaaggg aaggaggcac gtagcttata attaccattt gatagccatt cattatacat
    12901 aaaatcccac gcatcactat attcagattt tgaaatatca aaatgtccca caagatattt
    12961 tccagaaggt attatcatat tgccaagatt gtcattctct ttgacaacaa tgttatctgg
    13021 aatggtcatg caaatactag tccttaattg atgctcctgt gtaaattgtg gattatcatg
    13081 ataaatggct aatatttgtg tgttttcaag tccaagtaga ttctgctcac ttgcatatgt
    13141 gaataaccgt tctaaaagtt ttgggaaagt actggttaga ttaccatatg aaccagtata
    13201 cctagtgtaa atagtattca tattatcaag ttctagaatt tcaatttcac ctttaacctc
    13261 tctagtattc ttacatttag gttttgatat gcttctattg tactgagaaa ttttatatgg
    13321 ttctttgcaa ttcttgctat aattgtttct atattttata gggcttattt tatagtaatt
    13381 tttaaatgct cttgagaaaa ctgctgaatc agtaaagcca aagtagtgtg caatatcagt
    13441 aacagtcatg tcagttctat gaataagaaa agcagttgca ttttctagtt ttagcctatt
    13501 tacatattgt gccaaagttt ctttttctat tgctttgaag attctatgaa aatgaaattt
    13561 tgaaaaacct gctatattag aaagttcatc aagagaaaga ggttcatgta aatgttcttc
    13621 tatatagtct tgcactttgc aaatacgatt tagatattct tttttacttt tagcatttgt
    13681 catatatagc cctccttatt gtgtaaaatg tgtatcaatt aatttatagc aatatagtaa
    13741 taaaatttta tgtttttgta atttatgata aataaga
//
LOCUS       DAMJZE010000105        13224 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJZE010000105 DAMJZE010000000
VERSION     DAMJZE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.106"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            134..1993
                     /locus_tag="PC541_002540"
     CDS             134..1993
                     /locus_tag="PC541_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2839996.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2285..2884)
                     /locus_tag="PC541_002541"
     CDS             complement(2285..2884)
                     /locus_tag="PC541_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2839997.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2987..3784)
                     /locus_tag="PC541_002542"
     CDS             complement(2987..3784)
                     /locus_tag="PC541_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2839998.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3897..4002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4094..5080)
                     /locus_tag="PC541_002543"
     CDS             complement(4094..5080)
                     /locus_tag="PC541_002543"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2839999.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5564..7294
                     /locus_tag="PC541_002544"
     CDS             5564..7294
                     /locus_tag="PC541_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2840000.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7404..7484
                     /locus_tag="PC541_002545"
     tRNA            7404..7484
                     /locus_tag="PC541_002545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7438..7440,aa:Leu,seq:tag)
     gene            7819..8334
                     /locus_tag="PC541_002546"
     CDS             7819..8334
                     /locus_tag="PC541_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2840001.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8826..8930
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9140..11614
                     /gene="gcvPA"
                     /locus_tag="PC541_002547"
     CDS             9140..11614
                     /gene="gcvPA"
                     /locus_tag="PC541_002547"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2840002.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11614..13071
                     /gene="gcvPB"
                     /locus_tag="PC541_002548"
     CDS             11614..13071
                     /gene="gcvPB"
                     /locus_tag="PC541_002548"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2840003.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4958 a         1576 c         2183 g         4507 t
ORIGIN      
        1 aactatgtaa aacaaggttt atatattcta attgtataga aaaatttatt gtaaacgcat
       61 gtatattatt agaaaaaata gtttggaggt attcaataaa cataaaaaac aatatagggg
      121 ataaaggtgt tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt
      181 gatttttata agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt
      241 gattaattat gttgggattg ttcgtggagc tagccaacgt ctaactaaac tagagatgaa
      301 tcatcaacca aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac
      361 agggcatgga gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact
      421 tttattagaa aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga
      481 acagaataac cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt
      541 gtttaaaata gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat
      601 tatatctatt ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat
      661 ggttagactg gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac
      721 aggagtaaat actatagaaa aatttaaatt ggatgcaaat caaaatattt gtatacataa
      781 ggataagaaa tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat
      841 atttggatat gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga
      901 catagggaaa tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg
      961 ctatgtagat aaggaagatt tagtggtaag acaacgagat gtagacaatg aactaattaa
     1021 tataacaaat gaaattaaaa ataaacatag tattactgtt gttagtggaa tttgttgtat
     1081 tgaagatgta aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca
     1141 aaaaactgtt aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa
     1201 aaaaatgatt gaagaaaata ctatcaaaag tagaatacat gaagctattg aaaaaagaga
     1261 atttatagtt tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga
     1321 agctttagtt cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc
     1381 agtacttgag aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg
     1441 taaatggatt agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt
     1501 ttcaagaata caattttata acactaaatt tgtagaaact tattccaata tacaaaataa
     1561 atacaggata cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa
     1621 tcaaaaacat cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt
     1681 agatgatttt ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt
     1741 tttaaagctt gattctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt
     1801 aataaaaaat attgtacata tgttaaaaga acttaatatc acaactgttg ctgagggcat
     1861 tgaatgcaag gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt
     1921 tgtattttat aaaccaatgc caatattaga atttgaaaaa atattggata aagagtttgt
     1981 ttataatagc taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac
     2041 tgaataaatt tttactttat ttacagaata atgattattt acagaaaagc gcgaaaagtg
     2101 tattccttta agtgtgggat ggatagcaca aatagaggag aaaaaatatt ggccatataa
     2161 gaatgcataa taatataaat atttaaaagg tcagcattat ataaaaatta aaaagaaaat
     2221 aaataatttt tctataaaaa agagactatg agaaaattta ctcatagtct cctaaataat
     2281 gtatttattt tcttcctgca acccatttta atccccaatg aaattcagta tccatttctc
     2341 tatgaccttt gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt
     2401 ctatcaatgc tattgaacac atattatagc catttttatg ttcatcaagt ttgacaacta
     2461 agtctgaatc ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta
     2521 caccttcgta tataaaagca taaataagta ttctttttat atcttttatt ttattaccat
     2581 ttattcttaa attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag
     2641 caaaaggtgg attgtctaaa ctaccaaagg catttcctaa tgcttgaaca gcacctttta
     2701 caccattttt catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag
     2761 agccaaaaaa accctttttt tgtacttttt gattccaatt taaattaact aaaatttcac
     2821 ctaaatcgct attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag
     2881 ccatagtaat cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa
     2941 tattcaacaa gattagaata ataatattaa aattatacta aatatactaa aaagtaggaa
     3001 tcacaatacc tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat
     3061 cttgtagaac tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg
     3121 catactcgtc atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag
     3181 gtacagcata agtagtatct ataaatgcag cagtcacatc tggaagagaa gatggaattt
     3241 gagcaatatc taactctttg aattgtattc cctttggatt ttctattatg tccttaggac
     3301 taactaatcc atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt
     3361 tcttagaaca agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat
     3421 caacagattt tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag
     3481 ccattatttt gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata
     3541 gatttgcatc aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt
     3601 ttttaacttc taaagtatat cctttttctt taataagtgg ttttaattct tctaataact
     3661 ctcctcctgg aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac
     3721 atccaaccac agctaaagtt agtactagac ataataacaa acataataat tttttgaatt
     3781 tcatataaat tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc
     3841 acactgcaaa ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact
     3901 catctttcag tataatactg caggatttag caccaatgta tataaaaata tactggttgc
     3961 tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat
     4021 gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa
     4081 tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac
     4141 ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat
     4201 gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc
     4261 caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc
     4321 caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat
     4381 cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca tgctcactca
     4441 aacaataatt agtattatca gtatttgttt tagaaagata atccattata gcatctttaa
     4501 attctaatat gtccattttt gaatgtaaat gttcacttat attggttaca cgagatttta
     4561 cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt
     4621 catttatttc agatgaaaat aaaagagtcc catgatgcat tactttgttt ttgtagttgt
     4681 attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt
     4741 cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat
     4801 cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa
     4861 atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt
     4921 ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc
     4981 tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat
     5041 taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga
     5101 attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac
     5161 tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc
     5221 aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat
     5281 aaggagttaa gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat
     5341 gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt
     5401 tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt
     5461 gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa
     5521 taccctgtac agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat
     5581 taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga
     5641 agaagatata gacactattg cactagccaa tgcagaaaaa tttgggattt taactttaat
     5701 accaccatta gttgctataa tactcgcatt tataactaaa aacgtaataa tttctttact
     5761 tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac
     5821 atttatacag gcatttttag atttagtaga tagagcttta gtatctttag cagacccttg
     5881 gaatgcgggg ataatactac aggttttgtc aattggagga gtaataaatc ttgttgctaa
     5941 aatgggagga gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg
     6001 gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc
     6061 actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat cgagagaaaa
     6121 gctagcattt ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac
     6181 atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt
     6241 agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt
     6301 agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga
     6361 gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga
     6421 tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg
     6481 aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg
     6541 aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat
     6601 aaaagaagca tttagtgcat ctgatgcatc tagagcatta ttccagtctg cacttgttgc
     6661 aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat
     6721 tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc
     6781 atggtcatta agttcagtaa taaaagagtt aggtacagct aaatttttaa tacacctact
     6841 atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat
     6901 atcttttgct actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc
     6961 attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt
     7021 aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa tactttcttc
     7081 aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt
     7141 tacagcagta atcactattg tatttgggta tataccagct ggacttggat tgccaatata
     7201 tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa
     7261 agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat
     7321 tatcactatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat
     7381 atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag
     7441 gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat
     7501 tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt
     7561 tgtatatata atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta
     7621 catttatttc tgttttatta tcattcatct attagaatat actttagtta agaatgtgtt
     7681 aataagcagt gttcacattc ttgataaaat gtggaataat atgtattttt actaagacgg
     7741 aaatgaaata attaaaccat taaataatat tataagatga tgaataagta gataaatcaa
     7801 attttgtagg aggatattat ggaaaaatat atatggttgt ttccattact ttttattttt
     7861 catgatatgg aagagataat aggttttgga atatggctta aaaaaaataa atcaatgctt
     7921 gataagaagt atccatttat cagtaaagtt tatgaaaatt atagtactga aggcatggca
     7981 tttgctgtgt ttgaagaatt tattttatgc attattttct gtatacttac agttataaca
     8041 gagaatcagt atgtatatct actatggtta ggtagtttta tagcatatac cttacatttg
     8101 gtaattcata tagggcagtc aatcataata agaaaatata taccttcact tattaccagt
     8161 atcatatgct taccaataag catttggtgt ataagtaaga gtatttatat agttgattgt
     8221 gaaatgagta caacaattct gtacagtata attggcatta taattgtagc attgaattta
     8281 aagtttgcac agtcgctgat tggaaaattt acaaagtgga tgtcaaacat ataaatttta
     8341 gtttattgga cagttcaagg aatcagaaat tggaatttta tagtattaat attttatggt
     8401 attaatataa tgtataaaat tttatagcaa tgtatttcat aagtataaag tgactagaat
     8461 tttaagaaat atacaaattc tataaaaact aaataatttc aagtatattt taaaaatctt
     8521 atttttcaca agtttaaaat tgtaaacgat ttcatcaagt ctatgaatat acttttaaca
     8581 tgttatagta aatctcaata taaatttaca tttggttctt tgtgtgttat tttaataata
     8641 aattaagtga aaatgttatt ttacattaat atataatatc tttgcataag agtgaataat
     8701 ataaaagaga aataattgat aataaaaact taacaaaaaa attaatacat aaaaaaaaat
     8761 aaatataatg tgttgacatt taattgaaat attcatacaa taaaattgaa aataaatatt
     8821 tccaaatgag attagcgaga gagtccttta tacgaaaagg cgccgaagaa gaaatttaag
     8881 aaatgccaat ttttagagaa gctttcaggc aaaaatatcg ctaattgatg ggactctgga
     8941 gagacttaag ttaagatact ataagtcacc gaagaagcaa aataatatta aattattaaa
     9001 tctttcaggt atacaaacag ggatatatag gctaggatta ttttccatgc tgatatatct
     9061 tttttttatg aaaaagtaca ggaaataata tcaaagttac ttaaatacag taggtctaat
     9121 tttctaggga gggttatata tgaatgaatt gaaaagagta tctttgtaca atatacacaa
     9181 agagcttgga gcaaaacttg ttgaatttgc tggttgggaa atgcctttag aatatgaggg
     9241 tataaataaa gagcatgaga aggtcagaaa aagtgcaggt atatttgatg tatctcatat
     9301 gggagaggta cagataaagg gagcagagtc agaaaaattt atacaaaacc tagttacaaa
     9361 tgatataagc gcattaaaga taaatgatat tatatataca cctatgtgtt atgaaaatgg
     9421 tggagtagtt gatgatttat taatatataa atttggtgaa gaagattatt tattagtaat
     9481 aaatgcaggt aatatagata aagatgtagc atggataata aaacaaagtg aaggatacaa
     9541 tgtagatata aaaaatatat ctagtgaagt gagtcaatta gcaattcaag gacctaaagc
     9601 agaagaaatt cttcaaaaaa tcacagatat agacttaaac tcaataaaat tttataagtc
     9661 aattccatca acaaaagtat gtggttgtcc ttgtttagtt tcaagaacag gatatactgg
     9721 ggaagatggg tttgagatat actgtaaaaa taaatatgta gaaataattt ggaatgaagt
     9781 tctaaaggtt ggaggagaag atatatgtcc agctggtcta ggttgtaggg atactttgag
     9841 atttgaagca gctctacctc tatatggaca tgaaataaat gaacacatat ctccaattga
     9901 aggtggttta tctatctttg tgaaaacaaa taaagaatca tttattggca aatcaatttt
     9961 atcaaaagaa aaagaaagtg gagctaaaag aaagttagta ggatttgaaa tgcaaggtaa
    10021 aggaatgcct agaaatggat atgatataag gattggtgat aaaacagttg gatttgtaac
    10081 aacaggatgt gcatctccaa cgacaggaaa aattttaggt atgggaatta tagattctga
    10141 atatgcaaaa gtaggaaatg aaataggtat agcaatcaga aaaaaagttg ttcctgctgt
    10201 gatagttaag aaaccttttt ataaaaaaca atataaaaaa gataatatta tattaaacaa
    10261 agaaaataaa ttttcatata tacctgctac aagtgaagat aaatcaaaaa tgttaaaagt
    10321 agttggatta aattcagttg atgaattatt ttcagatata ccagaagaag taaaattaaa
    10381 aagagattta aatttggaaa ttggaaaatc tgaattagag gtaagcaaaa tagtaaaaag
    10441 attgtcagaa gaaaatttaa gtttagaaga cttgacttgt tttcttggtg ctggtgcata
    10501 tgaccattat ataccatcaa ttataaaaca tataacttca agatctgaat tttatacagc
    10561 atatacccca tatcaagctg aaataagtca gggaacttta caagtagttt ttgaatttca
    10621 atctatgatt gcagaaatta caggtatgga aattgcaaat gcatcaatgt atgatggagc
    10681 aactgctgca atagaagcat gtataatggc aatgaatcag actagaaaaa gtaaaatagt
    10741 agtgtcaaaa actattcatc ctgaaactct gtcagtatta agaacttact tacaatataa
    10801 agattgtgag atagtagaaa ttgacttttg taatgaatat gggactacag atatagaaaa
    10861 attaaaagct tctgtagaca aagatacagc atgtgttctt atacaaactc ctaatttctt
    10921 tggaattatt gaagaaatgg aagaaataga aaagataact catgaaaata aggctatgtt
    10981 aattatgagt gtagacccaa tatcattagg agttttaaaa acaccaggtg agataggtgc
    11041 agatattgtt gttggagaag ctcaatcact tggaaaccct cttaattttg gaggtcctta
    11101 tgtaggattt ttagctagca aatctaaata tactagaaaa atgccaggaa gaatagttgg
    11161 gcaaagttta gatgtagaag gtaaaattgc atatgtatta actttacaaa ctagagaaca
    11221 gcatgtaaga agagaaaaag caacttctaa tatatgttca aatcaagctt taaatgctct
    11281 tgtagcttca atatatatgg ctactatggg taaagaagga tttaaagagg ttggtatgca
    11341 gtctatgaaa aaagcacatt atacctacaa taaacttgta caaactggaa aatataagcc
    11401 aatatttaaa ggtaagtttt tcaaagaatt tgctgtacaa ggaaatctta atatagaaac
    11461 tataaatgat aaactcttag aagaaaatat attaggaggt tataatttag aatacaatta
    11521 tccagaatta aaaaattcaa ctttgctttg tgtgactgag aaaagaagca aagaggaaat
    11581 agataagtta gttggaataa tggagggatt ataatgaaag aatacaatag tttgttgata
    11641 gatatatcta aaaaaggtag gaaagcatac tcacttccaa agttagatat agatgatata
    11701 aaaatagaaa atatgattga ccaagatatg gcaagacaaa gtgagttgaa tttgccagaa
    11761 gttggtgaat tagaattagt tagacattat acattattat caaataaaaa ctttggagtt
    11821 gatacaggtt tttatccttt gggttcttgt actatgaaat ataatcctaa aataaatgaa
    11881 gatatggcag cacttccaaa ctttactgga atgcatccat accaatcttc ggatacagct
    11941 caaggttctc tttctttgat gtatgattta tcaagaagac ttgcagaaat tacaggtatg
    12001 gatgaagtta cactacaacc ttcagcaggt tctcatggtg agtttacagg gcttatgatt
    12061 ataaaagcat atcatgaaaa tagaggtgac cataagagaa ccaaagtgat aattccagat
    12121 tctgcacatg gaacaaatcc agcaagtgca gctatggcaa actttgatgt gattcaaata
    12181 gcatctgata aaaatggagc tgtagacata aatgcattaa aagaggtttt gaatgatgaa
    12241 gtagcagctc ttatgcttac taatccaagt actcttggtt tatttgaaaa aaatataaaa
    12301 gaaatagcta ctcttgtaca tgaagcagga ggacttttgt actatgatgg tgcaaacatg
    12361 aacgctatta tggggataac aagacctgga gatatggggt ttgatgtggt tcatcttaat
    12421 cttcataaga ctttttcaac tcctcatgga ggtggaggtc caggagctgg tcctgttgga
    12481 gttaaaaaag aattagtacc ttttttacca ataccagtaa ttgaaagaaa agaagataag
    12541 tatgtactaa attatgatag agaaaaatct attgggaaaa ttaaaaattt ctatggtaat
    12601 tttggagttc ttgtaagagc ttacacatat atactaacta tgggaaggga tggacttaaa
    12661 gaggctagtg aaatggcagt tttaaatgct aattatataa aggaaagtat aaaagatgat
    12721 tatattttgc caatagatac cttgtgtaag catgagtttg tattaggtgg attgccaaga
    12781 ggagaggcca atataaaaac tatcgatatt gcaaaaagac tactagatta tggataccat
    12841 cctccaacaa tgtactttcc tcttattata aatgaggcac ttatgataga acctacagaa
    12901 agtgagagta tagagacatt agatagtttt atagaagcta tgaaaagtgt tgctaaagag
    12961 gcaaaggaag aaccagaact attgaaaact gctccacata atactttggt caaaagagta
    13021 gatgatgcaa gggcagtaaa gaaacctata ctaacatggt cacaaagata aatagttaaa
    13081 aagtagagat ttaaagtata aagagaatta aatttgtaag aaaaagctat ctaacttaat
    13141 ctagtttaga tagctttttt gtatattagt atgtatggta ttttctatat aaagttttaa
    13201 attatattag tgcttatata gaat
//
LOCUS       DAMJZE010000106        13140 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJZE010000106 DAMJZE010000000
VERSION     DAMJZE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.107"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            216..1097
                     /locus_tag="PC541_002549"
     CDS             216..1097
                     /locus_tag="PC541_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2840004.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1305..2168)
                     /locus_tag="PC541_002550"
     CDS             complement(1305..2168)
                     /locus_tag="PC541_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2840005.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2323..4257)
                     /locus_tag="PC541_002551"
     CDS             complement(2323..4257)
                     /locus_tag="PC541_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2840006.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4369..5382)
                     /gene="galE"
                     /locus_tag="PC541_002552"
     CDS             complement(4369..5382)
                     /gene="galE"
                     /locus_tag="PC541_002552"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2840007.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5406..6275)
                     /gene="galU"
                     /locus_tag="PC541_002553"
     CDS             complement(5406..6275)
                     /gene="galU"
                     /locus_tag="PC541_002553"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2840008.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6473..6793)
                     /locus_tag="PC541_002554"
     CDS             complement(6473..6793)
                     /locus_tag="PC541_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840009.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6783..8774)
                     /locus_tag="PC541_002555"
     CDS             complement(6783..8774)
                     /locus_tag="PC541_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840010.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8897..11440)
                     /locus_tag="PC541_002556"
     CDS             complement(8897..11440)
                     /locus_tag="PC541_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2840011.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11718..12052)
                     /gene="rnpB"
                     /locus_tag="PC541_002557"
     ncRNA           complement(11718..12052)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC541_002557"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12184..12861
                     /gene="srtB"
                     /locus_tag="PC541_002558"
     CDS             12184..12861
                     /gene="srtB"
                     /locus_tag="PC541_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2840012.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(13021..13095)
                     /locus_tag="PC541_002559"
     tRNA            complement(13021..13095)
                     /locus_tag="PC541_002559"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13061..13063),aa:Thr,seq:tgt)
BASE COUNT         4048 a         2297 c         1340 g         5455 t
ORIGIN      
        1 ttttgtttat agattttatt tataagataa ttcttgtatt tttctattag gtcgtataaa
       61 tatatttatc ttataattca gacaaaaaac gataaatttt tttaaaagct tttattctta
      121 ctctataatc atatataata aaaatgtaaa aatatcttaa aggttaactt ctacaaatat
      181 ataaataaaa atacctatga aaggatgaac aaaaattgaa tttatatcac ttacgctatt
      241 ttgttacatt ggcacattta gaacactata caaaagcggc agaaaattta tctataacac
      301 aaccaagctt aagtcatgct atttctttac tagaaaatga acttggggtg gctctatttg
      361 aaaaagaagg tagaaatata gtccttacta agtatggaaa aatattttta aaagatgtgg
      421 aaaaatcatt agaaatttta gattcaagtg taaaatcatt aaaaataact ggtactggtg
      481 aaggacaaat agacttagct tttcttagaa cacttggtac tgattttatc ccagacatag
      541 tgcacaaatt tttaaaatct aatcctgcta aatcaataga ttttaaattt catactggtg
      601 taactactga cattattcaa ggtcttaaag aaagaaaata tgatattgca ttttgttcga
      661 aacttgaaaa agaaaaggga attgaattta tacctgttgc caaacaagat ttagtactaa
      721 ttgtaccata tagtcatcca ctagctgcaa aggatactat agatttaaaa gaaaccatac
      781 cttatcctca aatagtattt aatcagagaa gtggtcttag atatataatt gatgatatgt
      841 ttaaaaaaat aaatcaacag cctaatatag tctatgaagt agaggaagac caagtaattg
      901 ctggattagt tgcaaaaaat ttcggaattg ctgtagtccc aaatatgaat atgcttagct
      961 ttacaaaagt aaaagtaatt cagattattc acccaagttg ggaaagaaac ttttacttag
     1021 catttataaa agatagatat ttgcctcctg caattaaaaa ctttaaaaac tttgtcatta
     1081 aaaacgctca attataattt attaataaag gggttatttt taaatatcct cttttattct
     1141 ttttataaaa taaaaaaatc tatactttta aaagtataga ttcctaactg acagtcggca
     1201 tccgagtctc agtaattatc gttatacaac agcaactgga ttcagttcct gaataactag
     1261 gttacttaca ttatatcaga ttttttaaaa tttgtcaatt gtatctaacg cattttttca
     1321 agccatggtc caacaggctc tacaatagga acttctttaa atccccaatt tcttatatca
     1381 ttcacactcg ttggagtata tattttgttt ctgacagatt tgaatatttt gtttttttct
     1441 ccatattgca tacactttgg attagcttct attaaccctc ttcttgctct gctaagagga
     1501 ttacataaaa tatctgctat ttctaaacct tctctatagt cagcatcttc ttttttggca
     1561 attataaaac ccttaacctt atctcttaat tcttcatcta tatctaagta tattgttcca
     1621 ttattaaata catcaaaaaa cgccttttgt acttttaagt ttgagacatc atcccttgac
     1681 tctataacta ttcgtgcaga ttctacagtt tcattattta caaatgaata aaaattctga
     1741 agaacatgta taaaagccac aacatacggt tcctttgaag gtgcataata tttctttaat
     1801 ctagctttat ctactgttac tgatattata gtacactcta atctagatag aaataaaggc
     1861 aactcatcat agaataactt aatctgtgaa tcatcaaact tctcaaatgc tcttgatttt
     1921 cttgatatat ctgtcaaatg tattcctatg ttactggttc caaaacaata ctctttaaat
     1981 gcatccattt ctttcctcaa agaacataca taatcgcctt gacttactgc atatttatat
     2041 tcaaaaatta ctcctgtcaa tgtaaatggg tcttcatgat ttatatcagc tcttccactt
     2101 tcatcaataa acattatata ttttttcttt ataatcttac tcaccctctt aacatatgat
     2161 gatagcatct atattcattt tatatgagtt ttaaaaatat tccaagattt ttatataact
     2221 taaacttaat tttatactaa aaaattgaga tgtaaaacaa tcaagctttt attgattgaa
     2281 tttcacatct caattataat tatttttcaa tatatatttt tactattttg caagaatatc
     2341 cattaattta tccattacta tataagatac tccgcctcct atttgataag gagtttcaac
     2401 ttttttattt gcaaatactc tttgttgctc tgatgataat cctgaattat ccattaaaac
     2461 tattggtgaa tgtaatttta cagctaatgg tccagctgat aaagcatcta ctaaagcatc
     2521 tgatttacta actaatacag cctgtaaatc agtatctcca tagaattttt ctatcacttt
     2581 ggcattagta tctactctac tatctccata tattctattt cctgaaatat cttcagtagt
     2641 tatatcattc atttttgcta ttactgaatc gttaattgca cttggtccac ctataaagta
     2701 tgcattttct aattttcttt ctgttagcca tttataagat tcttctgtaa ttccatcttt
     2761 attgactaat attataggtt gtttttcttc cccagcttta gaagctacag ataaagcatc
     2821 tgcctcccca tttccagaaa ccatatatgc agtttttatt tcttctcgat ttccaagttc
     2881 ttcagctatt cttgtagcag tttgatatct atcttgacct gctattctct ttgtttctgt
     2941 gttgaaagta gtttttattt cttgctctaa tttagaactt attgcatttt ctccacctat
     3001 caagattact gttttaggtg ctaataactt catttcttca acagtttcag ttggtaaatt
     3061 agctgtatta gtaagtagta ttgttgagtc atagctactt gctaaaggag ttgctgttat
     3121 accatcagct attgcattac catttactaa tattacagta tctgaactat cagcattcca
     3181 gtttccaact gaacttatct ttttagctgt atcaaatcta tttgcaccta taattgtatc
     3241 ccatgtagga tttatagttc catcttcatt aaaagtataa tctttcccat ctattttttg
     3301 agaaccagtt agcatctttc catctttgtc taaatagtaa gacttaccat ctatttgttg
     3361 ccatcctgtc tgcattattc ctgttttgtc aaagtaatac ttattaccat ctatttcttg
     3421 ccatcctgtt actgctaaac catcagatcc tagatagtat ttctttccat tcttttcttg
     3481 ccatcctgtt tgcattactc catctgagtt aaagtaataa gtttttccgt ctattacttt
     3541 ccatccagat actcttgctc catcagttcc taaataatat tttttgttat taattgtttt
     3601 ccatcctgtt tgcattactc ctgaagcatc aaagtaatac atttttccat ttacttttag
     3661 ccaactattc ttttggtatg ttccatcaga ttttacataa tacttcttac cattttctgt
     3721 tttccatcct gtttgattta gatttatctt atattttgcc gctatcttta catagctgtc
     3781 gttactataa tctataatta catctgaacc tacaggaact tggtcaaata accatctaac
     3841 atctttattg tgcattctta tacatccacc agaaatatgc tttcctatag aactctcatt
     3901 attatttcca tgtattccat atgtatctcc ataagttcct tttaatgcta atcccatcca
     3961 tctatctcct aatggatttc ttggacttcc tccaggtata ttacctttat aatatggtct
     4021 attttttatc ttgttaacta cttttgtttt ccctgttggt gtttctgacc cctttttacc
     4081 tgttgcaact ctaaattctt ttacaagttt attatttaca aaatatccta gagtattttt
     4141 tcttgaattt actataatca catgtttact ggctgcgctt gattctggta cgccacatgc
     4201 aaatactaaa actaaagtca gtgctagtac taaatacttt tttagacttt ttttcactat
     4261 aacttccccc tcttttagaa acattactac atgtgtctaa ttataacata aaattaccaa
     4321 taccttatat ttacaaattt gttacattta tacatataag tatgtattct actcttcata
     4381 cccattagga ttcatacttt gccatctcca tgaatcttca cacatctctt caagttcata
     4441 cttagctttc catccaagct ctttttctgc ctttgaagaa tctgcataac acatggctac
     4501 atctccagcc cttcttccta caatcttata tggtatttct tttccacttg ctttagaaaa
     4561 tgcttttact aaatcaagaa cactataccc ctttccagtt cctaaattat aaacaacaag
     4621 tcctggattt tcttctaatt tttgtaatgc ttttacatga ccagcagcta aatctaatac
     4681 atgtatatag tctctaactc cagttccatc atgtgttgga tagtcattac cataaacact
     4741 caattccttc aatttaccta ctgctatttt tgttatataa ggcattagat tgctcggaac
     4801 tccatttggt tcttcaccta ttctaccact tttatgagca ccgactggat taaagtatct
     4861 taaaagagct atatccaatg acttatctgc ttttgatata tctactaaca tctgctctat
     4921 cataagttta gttcttccat atggattggt tacagaaagt ggaaaatcct ctaatatagg
     4981 acaagtatgt gggtctccat atacagtagc tgaagaacta aatacaaact ttttaacacc
     5041 atattctctc attaattcaa ataaatttaa ggtacttatt agattattgc tgtaatattc
     5101 aattggtttt tctactgatt caccaactgc ttttaaagct gcaaagtgta taattgattc
     5161 aatattattt tctttaaata caacatgcat ttcatcttta tttctgatat caatattata
     5221 aaattttact ggttttttag ataattcctt gattctatca accactattg agttgctatt
     5281 acttaagtta tcaactataa caacttcata acctgattct aaaagttcta ttgctgtatg
     5341 gcttcctata tatcctgctc ctccagctac taaaactgcc attgtaatta cctcttttct
     5401 aatatttatt tttcacttac tttttttctc atatagtcca ttaaatcatc ttttaaattt
     5461 tctctcttta gagcaaagtc tattgttgcc tctaaaaaac caattttatc tcccacatca
     5521 tatcttctac cttcaaagtt ataagcataa atagcctctt gttttccaag agtttgaagt
     5581 gcatctgtca gttgtatctc tccaccttta ccaggtgtct gtttctctaa tatgctaaat
     5641 attgcaggag ttattatata tcttccaagt atagctatat cagatggagc ttcttcaatt
     5701 gcaggttttt caaccatatc tttaacttta tatactctat cttcgatatg ttttacatcc
     5761 aatataccat atttattagt atcttcttta gctatctttt gaacacctaa tacagttgtt
     5821 ttatactcat tataagtatc tattaattgt tttaagcacg gaacatctga atctactata
     5881 tcatcaccta ataatacggc aaaaggttca tcacctataa aactttttgc acagtaaatt
     5941 gcatgtccaa gaccttttgg ctctttttgt cttatataat gaatatttac catatttgat
     6001 atgtctctta ccatttctaa catctctgtc ttaccttttt gctctagttc taactctaat
     6061 tcaactgatt tatcaaaatg atcttctata gattttttat ttctacctgt tattataaga
     6121 atttcttcaa ttcccgattc tatagcttct tctattatat actgtaaagt tggtttatct
     6181 actataggaa gcatttcttt gggctgtgat tttgttgctg gtaaaaatct agttcctagc
     6241 cctgctgctg gtattattgc ttttttaact gtcatctgca ttttttcacc cttcttcgta
     6301 aattttctat tgtaccaaat taatatacca tatttatact ttttcttcaa actctatatt
     6361 atcccaatat ttaatgctat aagataatat aaggtagatg aaatttcatc tacctttagt
     6421 tttataagtt aatattgtat tgtttatatt ttatccaaaa cttatacagg tactaaattt
     6481 catctaagat gccttttata tcactatcta tatcttcatc tagataagaa tctaaatctt
     6541 ctaagtcaac ttctagttct tgatatgtca tatcaacact gacatcctca tctatatcca
     6601 tttctgatat taattctcct aaaatactat ttccatcaga atctaaacca ctatctatat
     6661 cttcatctat atagttttca tttaaaattt catctaaatc aatctcgtaa taaacttcat
     6721 tatttttttc atcccctgag tccaatccta agaactcata gttaaaagtc caaacattgt
     6781 atttacttga catactttat tcctcctgtg aaaaacatat tttcatctag tttattggtt
     6841 ccataatcaa cagagttata tattttttca attatgtctt ttcttctttc ttttgtacta
     6901 ttatttaatg tcaattgttt aaatacatat gtatttgtta ttctctcgaa taatatttct
     6961 tttatactat catcatcaat atttctatag tttttccaca ataactcaaa taacttaaac
     7021 atttccctac tagtttcatc tacataataa ggatacatat tttctactaa atggtttaat
     7081 cttgatatgg cttgttttaa aaatacatca agacttgcac tttcactttc ttttaacatt
     7141 ctataattgt atttcttctt atcttctcct gaaattacaa agatgatatt gtctaaactt
     7201 ttttcaactt catttaaata actgcttata ttatatgata aatcagacaa tagattttta
     7261 atctgcttaa acacttgtac atcaagttta gtattatact tttcaaacaa atctatttct
     7321 tgattttcta atattcttgg catatatgct gacaataaat ctaaattgtt tctttgttct
     7381 tgtatttgct tataaagtga atttataact tctattctta tttcatcatt taactctttt
     7441 tctaaaatct tatcaaattc agatttcata tagtaaatat ttccaaagaa attaaatatt
     7501 atcatatcat tcatagcttt agaccaattc ttatatatca cttcaataga attttcttta
     7561 agtagaggtt gctttgcata tataaataca tcttgcataa gtttttgaac catactttta
     7621 cttaagttta aaagtacttt ttgattatta aattttattt ccgtttctac aaaattagaa
     7681 ttttcgtttt caagttcttt attaattaca atgtctttta tatcactatt ttttatagat
     7741 atgatttctt tttgaaaaga ctttagaata gtcaagctat cgaaatcttc ttgaataaaa
     7801 aatggagcat gttttaatgt atatcccact atttcatttt ctatatcttt taggtcatct
     7861 atttgcataa tttctctatt tttatcattt atatgtaggt taaatacatt tttattatca
     7921 tataatatta tatcttctcc taaatttaac ttagaacaat tatctttcgc tgactttaca
     7981 atagtcttct tagatttctt atttataaaa attaattggt cttgatattt tactaaaagc
     8041 atgtttgtgt ttttataatc aaatccatct atatttcctg atatcttgca gaatactatt
     8101 tcacaatctt taggaagatt tcctattttt tggttatctt taactctctc atttaaatca
     8161 gatgggacta cactatctat attgttatta aatatagata cagaccttac tataatcccc
     8221 tctctttcca tatgaaagta tcccttcaat gacactcttt tatcttctaa aatgtatctt
     8281 tctatatcat caaaatttat ttcaaatcta tttttcccac tgataaatat aaacttctca
     8341 ttatctttat taattccacc catataaaac ttattgtcat ctaaacttaa tatagtcata
     8401 ttatccttta gatagaatat aaattctctt tgacttttgc agattaaatc atattcatcc
     8461 aaagttattt tcatagtcaa atttgctgta acatttttac ttgaattttc actttttatg
     8521 taaaaaggca agttatttcc atagacaatt atttgagaat cttctaaaac tgcctctaat
     8581 ttttctttat tgtctgaaaa actatatata tattttacac ctgtatcctc attaacttca
     8641 tctgatttgc tgattattat attccctaga tttgttatat gaagcttttt atattctttt
     8701 gagtatctac ttatgccact aagctcaaac ccatctgaaa gctcatatat gattttttct
     8761 cctaatttca acataccttc accacctaaa cagaattata agtattttat aaaggtgaat
     8821 aaaatgtctc aaaacaaata atattttttt taatactttt tattctgaga catagtttct
     8881 ccttttttat aatgctctaa gcaccttcaa ctatttcttc ataaagttta tctaaatgtt
     8941 taacatattc acgatatcca gatttgttct ttaactcact tcttaaatct tcatattttt
     9001 gtttaagttc ttcagcactc aaagaaggag ctgattcttt taattcttct aacttataat
     9061 catagtactt atcatacata gctatatatg tatcaataga cttcttagct agttcattac
     9121 atttttcttg tgcttctcca tattgagaat aatcacctat ttttatttta tagtatccat
     9181 tttcttgaat tattgttgaa gaaatacctt tagcttcata ttcttttgct gtttcttttg
     9241 ctgttttttc aactttaagt gaacctgcat aaacagtgta atatacacct ttaccatctt
     9301 tatatgcttc catttctttt gctttctttt cagcttgtgc tttttcttga gcctcttttt
     9361 cagcttttgc cttttcttct gcttcttttt tctctttttc agcttgtgct tttttctctt
     9421 cttcagttaa tttattgtta gcttgttgtt gagttgatga ctttggaata ttctctttat
     9481 catttttctt attgttgcct actgttataa aatatcctac accaacaata accactataa
     9541 ctactgctat aatagtttct tttaatctac tcttcttaga aacaacctct tcatcatcta
     9601 aagaatcttc tttatctgat acataatttt tcatttcttt cttgttttct ctatatttgt
     9661 ccttagaaga atttttagat tttttattac tattttcttt ctttttaata ggtattacta
     9721 ctgatttttc attttctaat ttttcttctt ttatttcttt tgctttttta tcttctaagt
     9781 ttttactttc tttttcaagc ttcgattcat tatttgaaga tactttttca tctaagttat
     9841 tatttattgg tttttctatt tttacactac gttcttcttc tatatgttga cttggttctt
     9901 cttgttttat ttctttcaat tcattttgct ctatttcttc tgatttcact tgatgatttt
     9961 caactggttt atctaaaatt atatctggag aaatatcatt taaattctct tcttcttgtt
    10021 cagcttcttc attgtctttt ttaataaatc caaataattt agccttcttt ttactctttt
    10081 tattttctgg ttcaattata tcttctttat tgctgtcgta ctctttatct gtagtatctt
    10141 tatcttctac aacgtcacct atatcttgat tattactatc tacttcttct ttagtccctt
    10201 cttcagcatc atcttctatt gaaatactac ttctaaagca aatatcttta tctgcatttt
    10261 ttatcatgta aatgtattct tcaatatcga tatctttatt tcttctagtt ctttttgatg
    10321 caatttcatg attttctaat tcactatagt tatcttttat tagattctgt gctttattcc
    10381 atgtatcttc actaatttct gttttatttt taattacata gtccacaaat tccttaaaat
    10441 tttcaggacc aaattctcca attatagtca gtctcattgc accttctata aggtctctat
    10501 ttcttctctt taaaaattct aatacaactg ttgcacaagc aaaatcttta ataatttctt
    10561 tgccaaaacc atatgcttca acagaatgaa gttcttctaa tccacataac ttcttatatt
    10621 cattgtgtat tgtttgagat atactttcat tatttaagct atttaactta tctataaact
    10681 tcgattctat atcatttgat tttgattcat gttttacttt tgaattttta atttcattta
    10741 gtagcatgtc attttctgaa ttatctttac ctaattgcaa atcctcaatt tcagttgctt
    10801 ttatttctac tttgttttgt tcgttattct cttccacatt attaggtacc acatctttat
    10861 tactagctaa tattttgttt acacagaata taatttctct ttttaattct cttttcgcat
    10921 ctgcttgatt tttaagaaat aaatctccac attctttctt ttctaaactt atttttctaa
    10981 gagatatatt aagaaggtct ttatataggt ttaaaacctt ataatccttt tctaaacgtc
    11041 ttcttctctc tgatagttcc attttatcat aagctaaaaa actatttaaa tcattggctt
    11101 ttagagagtc taatccctct ttcatagcct tatttacata ctttctacat tttgctgtag
    11161 ttaactctaa atctttttct aattcatcta tttcaggtat gtacttatct actatccatt
    11221 tgccaataag tggttctgta tttcttgcta atgtcttata aaacaatata ttaaatttta
    11281 aaagctctgc atattttgct tcattaaatt ctactttttc aaatttcttt tccaaaacac
    11341 catctagctt ttcatttatt ttagactctt gttttaaaaa acttgctgtg acatttaaaa
    11401 cagtgtttct tattatatta ttttcactca aaaccttcac tccttaaatc aattatttta
    11461 ttgtaatatt tacattgtgc aaagttgcta atttgattat gatatctaaa taattattta
    11521 ctaaatttgc tacatcttat atcatatcat attatttaca tcattttact ttttcttttt
    11581 atatctatct tatattatac catttttaat atctgtacta acttatatta caatattcct
    11641 caaaatttta aaagtagatt agacaaattt acaaataaaa aaacatcagt ttaaactgat
    11701 gtttttgact atcgaaataa ttaagcaagt ctataagcgg agttctgtat tagataatca
    11761 tctttctagg cctattgtca ccaataggct caagcgacct acctaccgga gagtacgagc
    11821 agcactcttt attcctatct tggtcttgct ccaggtgggg tttacatagc caactagtcg
    11881 cctagctgct ggtgagctct tacctcgcct ttccatcctt accacaatag tggcggttta
    11941 tttctgttgc actatcctta ggatcgctcc cactaggcgt tacctagcac cctgctctat
    12001 ggagctccga ctttcctcat gtaagattac atgcgattat ctggcttact taatatttat
    12061 tttacttagt tatgttaaca caaattcttt tacatgtcaa agtaatatta tactaaaaat
    12121 ttaaatttat tcaaaaatag tgtaatatag atatataaac tacatttaac tcgaggtgat
    12181 ctattgaaaa aattatatag aatagtaatt aacattatac tagtattagt aatactttac
    12241 agtggattta atatatactc aaaacttacc aaatacaatc atgatactaa aatatcctca
    12301 gaacttcaaa aaaaagaata taaaaaagag gatttatcaa agattaacag tgattttaaa
    12361 ttttggcttt ctgttgagaa tacaaatata aattatccag ttgtacaatc taaagataat
    12421 tcttactatc ttgacaaaga tttttataaa aaagattcta tttctggtac actgtttatg
    12481 gattatagaa ataaatctat tgatgacaaa aatatcataa tatatggaca taatatgaaa
    12541 aataaaacta tgttcaacaa cctaaataag tttaaagatg ctgacttctt taaaaaaaat
    12601 aataaaataa aaataacctt gaatggaaaa gaatttttat atgatgtttt ttctgcttat
    12661 attgtagaat ctgattatga ttatctaaaa acaaatttta acaatgaatc tgattatcaa
    12721 aattacataa atgatattac ttctaaatca ttgtataaat ctcctattaa ggtaaatagc
    12781 aatgataaaa tagttacact ttctacatgt acctatgaat ttgatgatgc cagaatggtg
    12841 attcatggta gattgattta aaactcacag acacagttta aaacaataat tttaaactgt
    12901 gtcatctttt atacctataa tttgtaaatt agaccactaa aagtatttaa tacaaaataa
    12961 caaatacatc aaacaataaa aaacaataaa aaaagactat gctaaaaaca tagtctttac
    13021 tggagctggt gataggaatc gaacctacaa cctgctgatt acaagtcagc tgctctaccg
    13081 ttgagccaca ccagcacacc tattttattg taaattagca aaactaattt ttaaaagtta
//
LOCUS       DAMJZE010000107        13114 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJZE010000107 DAMJZE010000000
VERSION     DAMJZE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.108"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            89..553
                     /gene="bcp"
                     /locus_tag="PC541_002560"
     CDS             89..553
                     /gene="bcp"
                     /locus_tag="PC541_002560"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2840013.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            563..1306
                     /gene="yaaA"
                     /locus_tag="PC541_002561"
     CDS             563..1306
                     /gene="yaaA"
                     /locus_tag="PC541_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2840014.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            1434..4091
                     /locus_tag="PC541_002562"
     CDS             1434..4091
                     /locus_tag="PC541_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2840015.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4145..4247
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4357..5622
                     /locus_tag="PC541_002563"
     CDS             4357..5622
                     /locus_tag="PC541_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2840016.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5652..6542
                     /gene="metA"
                     /locus_tag="PC541_002564"
     CDS             5652..6542
                     /gene="metA"
                     /locus_tag="PC541_002564"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2840017.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            7033..7476
                     /locus_tag="PC541_002565"
     CDS             7033..7476
                     /locus_tag="PC541_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2840018.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7507..9324
                     /gene="ftsH"
                     /locus_tag="PC541_002566"
     CDS             7507..9324
                     /gene="ftsH"
                     /locus_tag="PC541_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2840019.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9502..12204
                     /locus_tag="PC541_002567"
     CDS             9502..12204
                     /locus_tag="PC541_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2840020.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12238..12936
                     /locus_tag="PC541_002568"
     CDS             12238..12936
                     /locus_tag="PC541_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840021.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         5147 a         1504 c         2397 g         4066 t
ORIGIN      
        1 ataaaatatt ttatttatat atagatttat agggtaataa tataagcata gtataaaatt
       61 attaataaaa acaaaaggag agatatttat gttgagtata ggaacaaaag caccagaatt
      121 tacactagaa gataaagatg gaaataaggt aagtatgtca gattttaaag gcaaaaaagt
      181 agtagtatat ttttatccta aagataatac accaggatgt acacgtcaag cttgtgcttt
      241 tagaaatgca tatgatggat ttaaaaagga agatatacaa gttattggga taagtaaaga
      301 tagtataaaa tcacatcaaa aatttgcaga aaagcatgaa cttcctttta ttttactttc
      361 tgaccctgac ttagttgcta taaaagcttt tgatgtatgg aaagagaaaa aaatgtatgg
      421 aaaaactgca ttgggagttg tacgtgcgac atatattata gatgaaaatg gaattattga
      481 aaaagtcttt gaaaaagcaa aaccagacac aaatgcacaa gagattcttg aatatttgga
      541 aaaacaggag taataagaat ttatgattat aatgttatca ccagctaaaa atatgaagaa
      601 tatagaagta tttgatagag atttgagttt gccttgcttt atagataaca caaaagaaat
      661 agtggaaaat ataaagacat ttggtataga agattttaaa aataaaatga aaattaatga
      721 aaaattagct gtattaaata aaaatagatt tgaaagcatt aaatttgata gattaggaaa
      781 ccctgcaatc ttaacttatg atggaataca atataaaaat atagaagcag aaaattttac
      841 aaggaaagat gaagagtttg caaatagctg tattcgaatc ataagtggat tgtatggtgt
      901 tgtaaaacca tatgatagta tatatgaata tagattagaa atgcagacaa aattacaagt
      961 aggtgagttt aagaatttat acgaatattg gggaaatcgt atatataaag aactaatcaa
     1021 agaaaaaaca gctatcgtta atctttcatc aaatgaatac agcaaatcca tagaaaaatt
     1081 tattaaagat agtgatacat atattacatg tacttttaaa gtaaataaaa atggcatatt
     1141 gaaagtggaa tctacacaag caaagaaagc tagaggtatg atgacaaaat acattgtaaa
     1201 aaataggata agagatattg aagaactgaa aaagtttgat ttagaaggtt ataagtataa
     1261 agaaaactta tctaacaata gtgaatacat atttgtaaaa gagtagaagt tttgttaaat
     1321 attaaatggc attgttaaat ttaggtaaaa tatacttagt gtgtattgaa ttgaacttga
     1381 ttttaaaata taattattct taaattaaaa aaagaaagga gtggtcttcc aaaatggaaa
     1441 atcacaaaca gatgccttgg cattcaagag caacgcatga aattttagaa agtctagatt
     1501 catcagagga ggggctcacc gataccgagg cggaaaaacg gcttaaagag aatggcttaa
     1561 atgaacttag aaaaaaacca cctagaacaa ctttacaaat gctttggagt caaattacag
     1621 atgctatggt tatgatacta ataggagcag caatattatc tttgatattt ggcgaattta
     1681 cagaagcttt tgtaatacta ataattgtaa cagtaaatgc agttattgga attgttcaag
     1741 agaaaaaagc agaatcatca ttagaagcat taaaaaatat gaattcacct acagctcgtg
     1801 ttatgcgtga tggtgaagaa aatgttattc cagcaagtaa tcttgtagta ggagatattg
     1861 tttttttaga agatggagct atggttccag cagaccttcg tttaatagaa acatcaagtt
     1921 taaaaattca agaggcttct ttaacaggag aatctgttcc atcagaaaaa gatgcagata
     1981 caatcttacc taaagaatgt gtattaggag accgtagcaa tatggcgtat acatcttcaa
     2041 tagtcacata tggtagaggt gtaggtgttg tagtagcaac tggtatggat acagaagtgg
     2101 gtaacatagc acatctttta gattcacaag atgattttga tactcctata aaacgtaaac
     2161 ttaatactgt tggaaagaca ttaagtgtca taggtattat tgtatgcgtt gttatatttg
     2221 caataggtgc attttatcag agacctctct tgccaatgtt tatgattgca atttcccttg
     2281 ccatatcaat tattccagag ggattacctg caacagctac tattgtaatg gctttaggcg
     2341 tacagcgtat ggctaaaaga aatgctttaa ttagaaaatt acctgctgtt gaaacattag
     2401 gaggagctac agtaatatgt tgtgataaaa ctggtacatt aaccttaaat aaaatgactg
     2461 ttacacatat tgctgtcaat ggagattttg aatctggaaa agtgacatca atagatactg
     2521 catctgaaaa acatccatta gtgtataaag agattgttta tgctggtgca ctttgtaatg
     2581 atgcatgtat taatccagat aaaacagatg aaatattagg tgacccaaca gagggcgcat
     2641 taatttttat ggccaaaaaa tttggaatag atcaagaagc tcttgaagaa gaatatccac
     2701 gtgtatttga acagcctttt gattcagata gaaaatgtat gacaactgtt cataatatag
     2761 atgaaaagat tattgcttat actaaaggag cagtagacga aattcttgac ctatgtacta
     2821 aaatattaac ttcaaaaggt gaaagaaaca ttactgaagt ggataaaaag aatatacacg
     2881 aattatgtct taacatgtct aaagatgcat tgcgtgtact tggttttgct aagcgtgaga
     2941 tttcatctat accaaaagaa gatagtgaaa atatagaata tgatttaaca tttataggta
     3001 tggttggtat gattgaccca ccaagaactg aggtaattga tgcagtaagt acttgtaaag
     3061 aagcaggtat aagaaccata atgataacag gtgaccataa agttactgcc acaacaatcg
     3121 ctcatgagtt aggaatatgg agtgaagaaa atactgtgat atctggtgat gaacttgata
     3181 atttgagtga tgatgaacta gatgaagctg ttaaaaatac tacagtattt gctcgtgtat
     3241 ccccatttga taaacttaga attatacaat ctctaaaaag aattggagaa gtaccagcaa
     3301 tgactgggga tggtgtaaat gactctcctg ccttaaagtc tgctgatatt ggtatagcca
     3361 tgggtataag tggtacagat gttgcaaaag attcttctga catgatttta atggacgata
     3421 gttttacaac aattgcttat gcaataaaag aaggtagacg tgtatataga aacatacaaa
     3481 aagttataca atttttgctt gttggtaata tagcagagat acttacgttg tttgtagcaa
     3541 tattattaaa ttgggatact ccgcttcttg ctgttcatat tctatgggta aaccttgcaa
     3601 ctgctacgct tccagccctt gctcttggag ttgacccacc aagcagaaat ataatgaagc
     3661 accagccagt gaaatcaacc actttgtttg aaaaaaatct tatacaaaga gttgttaccc
     3721 aaggtatatt tgtggcaata atgactattt tagcatataa aattggtctt aatatagaaa
     3781 atcattctgt aggtcaaaca atggcatttt gtgtattagc attttcacag atgttacgag
     3841 catttagtca gcgttcaaat acagattcaa tgtttaatag aaataatggt aaaaatccat
     3901 ttcttttaat ctcattttta acttctgcat ttttaatagc agtaatacta tttgtacctg
     3961 tttttagaaa tgcttttaac ttaacagttt taaataactt agagtggctt gtaacgattg
     4021 ttttagccat aatgtctgta attcaagtag aggttgggaa aattattaaa agaaaaaaat
     4081 taaaaaaata gttgacaaaa ttaggaaaaa tatataagat ataaaacata gaaaaattaa
     4141 ataaatctta tccagagagg tggagggact ggccctatga aacccggcaa ccagtatatt
     4201 tatatacatt ggtgctaaat cctgcggcga aagctgagag atgagtataa ttaagcttat
     4261 acagagcctg agttatatac tcaggcttta tttattttaa ctctcatctg gtaaatagtt
     4321 taattttttt tactaaaatc taagggggat acaagaatgt ataataaaga aacaatatgt
     4381 gtgcaaggaa attataaacc aggtaatgga gaaccaagag tactaccttt atatcaaagt
     4441 acaactttta aatatagcag tatagaccaa cttgctgaat tatttgattt aaaagttgat
     4501 ggacatatat attcaagaat aagcaatcct actattcaag cttttgaaga aaaaataagt
     4561 ttactagagg gtggagtatc ttctgtagct gtatcatcag ggcaatctgc aaatatgttg
     4621 gcagttttaa atatatgtaa atcaggagat agtatacttt gttcttcaaa agtatatgga
     4681 ggaacattca atttactagg acctagtctt aaaaaatttg gtatagattt aatatcgttt
     4741 gacttagatt caagtgaaga tgagatagta gaacttgcaa aggaaaatac taaggttgtg
     4801 tttgcagaaa cacttgcaaa tccaactctt gaagtcatag attttgaaaa aatagcaaat
     4861 gtagctaaga gaattaatgt tccatttatt gttgataatt cattagcatc tccagtgctt
     4921 tgtaaccctt taaagtatgg agcaaatata gttactcatt ctaccacaaa atatttagat
     4981 gggcatgctt caagtgttgg aggaattata gtggatggtg gaaactttaa ctgggataat
     5041 ggaaaatttc cagaattagt tgagccagac ccaacatatc atggtataag ctatactcaa
     5101 aaatttggaa atgccgcata tgcaactaaa gcaagagttc agttgcttag agactatgga
     5161 aattgtttaa gcccattcaa tgcgtatctt actaatttaa atgttgaaac actacatctt
     5221 agaatggaga gacatagtga aaatgcactt aaaatagcta gatttttaga aaaacatgaa
     5281 aatgtagatt ggattaatta cccaggactt gaagataaca agtattatga gaatgccaaa
     5341 aagtatttat caagaggatg tagtggtgtt ttatcatttg gagtaagagg tgggttagaa
     5401 aatgccaaaa aatttgtgga aaaattacag atagcatctt tggttacaca tgtttcagat
     5461 gtaagaactt gtgttataca tccagcttca actactcata gacaattaac agaagaacaa
     5521 ttaattgcat ctggagtatt gccttcacta ataagattat ctgttggaat agaaaatgta
     5581 gaggatttaa tagctgattt aaatcaagct ttaaatttct aatattctaa gagaaagaga
     5641 agtgaaaaga tatggcttta atactaccaa agggattacc tgttattaat aaattattag
     5701 atgaaggaat agatgtaatt tataaagaag attttaaaaa ggaattaaag tatgaagaaa
     5761 acatagacac taagatagct atactaaatc taatgcctat aaagatagat acagaattag
     5821 atttactaag aaggatagat aaaacaggat tcaacgtgtc tgtggaattt attaaaattt
     5881 caacaagaga aagtaaaaga agttgtaatg aatatgttaa agaattttat aaaacatttg
     5941 atgaagccaa aggagagtat tttgatggct ttataataac aggagctcca gttgaacaaa
     6001 tggagtttga agaagttgat tattggaatg aactagaaga aattatggat tattctaaga
     6061 gaaaaactaa atctacatta tatatatgtt gggcagctca agcaagtctt tacaaatact
     6121 acaatgtaaa aaaacttcca ctaagtcaaa aatgttttgg agtatttaag cataaggtag
     6181 ataaagatag taaaatagta gatggatttg aaaatgaatt ttttgctcca cattcaagac
     6241 atactactgt aaatattgaa gctttgaaag agaataaaga gttgagcatt gtgagtcatt
     6301 ctaaagaagc tggtccctat ataattacaa attcaagaga tgtgttcgta atgggtcata
     6361 gcgagtatga caaatacact cttgataaag aatataagag ggatataaat aaaggagata
     6421 aaatatctat acctcaaaat tactatatta atgatgaccc ttcagaagag ccaactgtaa
     6481 aatggaaaaa acattcagaa ttgttgttta gaaattggat aaaaaactat ttgatacaat
     6541 aaattttaag agtatataaa tgttaactaa aaaaatatag aaatttatag gtataaagtt
     6601 aatcttaatg atactttata ccttattttt ttaaaatttg accttataat agtagtttta
     6661 tattttaaga aaatcacttt aggtgtatgt agatagaggt ggctttaatt ttatatatgg
     6721 tgcgataatg ggagactaaa cctttatagt atcaataaat ttgtatttta acttttaaaa
     6781 gagattaaaa agggagaaat cagtgtaaaa tatatatata gattttttat taaattttat
     6841 taaaaatatc ctgtataaaa ttaaatatca ataagataac aaaatttcaa ttgaagttac
     6901 aatattgtaa ttgacaataa atacttaata ctataatatt taatacatta aacattatag
     6961 tgttaagtat ttatttacag tatgcacttc agcctaaatt tgaaataaag tattttcgta
     7021 gggagcgttt gtatggatga gatgagtttt aagaaagagc tactagagtt gactagagat
     7081 ataaatatga aatttacaac tttattaagt gatttttatc agccacttgg tattacagca
     7141 gtgcaagctt tgattttatc tgagttatgt gaacatggag agaaaaaaat aagcgattta
     7201 gggaagaact taaacatgac aaatagtaat gtatcagtaa tctgtcaaag gcttgaaaaa
     7261 aatggctttt taaatcgcat aagagatata gaagaccaac gaattgtaaa agtaaaagtt
     7321 acaaataaat cattggatat acaagaacat atttcaagta gtatttttga tagttatttt
     7381 gaaaatatga ccagtgaaaa attgcaagat atggaagata taatagaagg tttagagaaa
     7441 ttaaataaac ttttaactta cattgattgc aaataacata aaaataattt aaaggagaat
     7501 aaaattatgg atgaacataa accccaaaaa aggtcagcac tctattatta tacagttata
     7561 ataatagtag taatgttttt aaatgcttta atatttccaa gtttaatgga taaaaaaatg
     7621 gttgagattg actatggaaa gtttctcaca atgttggata caggaaaggt taaatctgtt
     7681 gagatagaaa ataatagaat agcaataaat ccatctagta gcaaggataa gaatatatac
     7741 ataactggta gaatggatga cccagaattg gtagatagac taaaaaatgc aaaagtagaa
     7801 tttactaagg ttattcctaa ggaaaattca cctcttttaa gtattttact tacatggata
     7861 atacctatag gtattcttat gctatttggt aacttaatga tgaagtctat gcaaaagcga
     7921 atgggtggta atgctatgca atttggaaaa agcaacgcca aaatgtatgt atctgctcaa
     7981 agtggaaaaa gatttaatga tgtagctggt caagatgagg caaaagaagc tttaactgaa
     8041 atagtagatt tcttacacaa tccagaaaag tataaaaaga taggagcaca aatgcctaaa
     8101 ggagcacttt tagtaggacc tccaggtaca ggaaaaacac tccttgcaaa agctgtagca
     8161 ggtgaagcaa atgtaccatt tttctcaata tctggttctg agtttgttga gatgtttgta
     8221 ggaatgggag cttctaggat tcgtgattta tttaaacaag caaaagaaaa agctccatgt
     8281 atagtgttta tagatgagat tgatactatt ggtaaaaagc gtgataatgg aagtggatta
     8341 ggtggaaatg atgaacgtga acaaacttta aatcagttgt taagtgagat ggatggtttt
     8401 gatggtggaa ttggagtagt aatacttgct gctacaaata gacctgacag tcttgacaaa
     8461 gcattacttc gtccaggaag atttgataga cgtataccag ttgaacttcc agaccttgga
     8521 ggacgtgaag ctatattaaa ggtacatgct caaaatgtaa atgtaagctc agatatagat
     8581 tataatgcaa ttgcaaggtc tacatcagga gcatcaggtg cagaacttgc aaatattgta
     8641 aacgaagcag cacttttagc agttaagtct ggcagagata tagtagaaca acaggatttt
     8701 gatgaatctg ttgaaactgt aattgctggg tatcaacgta aaggagctgt aatttcagat
     8761 aaggaaaaga aaattatagc atatcatgaa ataggtcatg cacttgttgc tgctatgcaa
     8821 aaacattctg cacctgtcca taagattact ataataccta gaacttcagg agcattggga
     8881 tatactatgc aagttgctga agatgagagt gtacttatgt ctaaagaaga agcaattgat
     8941 aaaataacta cttttacagg tggtcgtgct gctgaagagg ttatctttaa cacttgtaca
     9001 tcaggagcat caaatgatat agaacaagct actaaaattg caagagctat ggttactcgc
     9061 cttggtatga gtgaagagtt tgatatggtc gctcttgaaa ctgtaagcaa tcaatattta
     9121 ggaggagata cttctcttgc ttgttcttcc gacactgctg ctaaaataga tgaagaggta
     9181 tttaaaatag taaaggcttg tcacaaaaat gcaattgaaa tcttagaaaa taatattgat
     9241 aaattgcatg aacttgctaa ttatttatta gaaaaagaaa ctattacagg tgatgagttt
     9301 atggaaatac ttaacttaaa ctaaaatgat tatacacaag ataaaaatgc ctattgttag
     9361 gcatttttat tttacaacta aattttttaa atttatattt aaatctttac ataatcttaa
     9421 tcattaaaaa tacatcttaa ctacaatttt atatcggaat tgatattata atagtaatga
     9481 atggtgtaag gaggtttttt aatggataga cctaatcctg atattctatt agaaaaaatt
     9541 aaaaatgaag aagaaaaact aagtagaggt caattaaaaa tattctttgg ttatgcagct
     9601 ggagtaggaa aaacctactc tatgttggaa tctgcacaaa atttaaaaaa agtgggtgta
     9661 gatgtagtag ttggatatat tgaaccacac actagaccag aaacattggc tttacttgat
     9721 ggcttggaaa ttcttcctgt aaaggagata gaatataaat ctataaaact aaaagaattt
     9781 gatttagatg ctgctttagc aagaaaacca gaagttatat tagtagatga atttgcccat
     9841 tctaatgttg ctgggttaag gcatacaaaa agatggcaag atatagaaga attgctgtta
     9901 gcaggcataa atgtctacac tactgtaaat gtacaacatt tagaaagtct aaatgacatt
     9961 gttgagataa ttacaaacgt atcagttaaa gagactatac cagataagtt tttggataca
    10021 aatacacagt tagaacttat agatgttgaa cctgatgtac ttcttgaaag gttcaatgaa
    10081 ggtaagatat ataagaaaga acaagctact agagcaaaaa ataatttttt tataaaagat
    10141 aacctggttg cacttagaga aattgcactt agaaaaacag cagagagagt taataaagaa
    10201 gttcaaatga ccagactatc aaagggtgat gtaactgtaa tacctacatc agatacactt
    10261 cttgcatgta taagtccatc tccatcttca gcaaaagtta ttagaactgc atccagaata
    10321 tcagacagta catttgcaaa atggatagct ttatatgttg aaactccaaa cacagcaaga
    10381 cttccatttg atgaacaaaa acaactacaa aataacctaa aattagctaa gaaattaggt
    10441 ggagaaataa ttgttttaca tggtgaaaat ataatagagc aaatacttag aattgcaaag
    10501 ctgagaaatg taacaaaaat tgtaataggt agaaatcatt caagtaataa aagatttagt
    10561 aaaagattta aaaaagatat agttgacaaa ttaattgatg aagttgatta tatagatata
    10621 catataattc catataaatt tgcctcagat gttaaataca aacctaaaaa ggataaatca
    10681 tcaataaaat ctaaatttaa aattagcaaa gttgatttta taaaacttat atttattact
    10741 ttgatgataa gtattttagc atatgcagta caaagcatgg gatttataag agaaaatata
    10801 ttgttaatat atatgttggg tgtagtttta gtttctatgt ggactaaagg gtatagtaca
    10861 ggtataataa gttctgtatt taatatagtt cttttaaatt atttttttac agcaccatta
    10921 tatacattat ctatagcaga ttcaaactat atagtaacac tggttgtatt ctcaatagta
    10981 ggtataataa ctagcactct tacgtctaag atacagcatg aagcagaaac tgctgctaaa
    11041 agagaagaaa atacaaaaat gatatatcaa ataattaagg gatttttaag attatcaaat
    11101 aaagatgata tagtaaacaa aggtatagaa cttttgtctc ttagcttaag tagagatgta
    11161 atttgttatt taaatgtaga taagcataat aaatcaaagc tttacaaaaa aaatactaat
    11221 aataaggata aaaatgattt ggatagtgaa gatgaaaaag ctgttgcaag ttgggtttta
    11281 tccaattcaa cagtagcagg aaatgacacg gatacacttc ctggttcaaa gggatactat
    11341 ataccaattg taggaatgaa tttaacttta ggagtagtgg gaatatcatg tattgattca
    11401 aaattggata cagaagatat aagcttaatt gaaacaataa ttgctcaaat ggcaatagcg
    11461 cttgatagag aaatcttgtc agaagctaaa gaaaatacca atcttgagat tgaaagggaa
    11521 agattaagaa gtaatctttt aagagcagtt tcgcatgacc ttagaactcc actagcagga
    11581 atatctggtg cagtaagtac aattataaaa aacaagggta ctataggtca agatataata
    11641 gatgaattac ttagtggtgt atatgaagat actcagtggt taataagact tgttgaaaat
    11701 ttacttagta tgaccaagat agatgaaggg aaattggaag ttaaaaaaca ttctgaactg
    11761 gttgaggaaa ttatatcaga agcgttacaa aaaataaaaa aaagaattga aaatgcactt
    11821 atagatatta atataccaga acagatatta tttgttccta tggatgcaaa attaatagaa
    11881 caagttttaa ttaatttaat agataattct ttgaaatatt caaaggaaga ttgtaagata
    11941 gagataaatg tatatgagaa agatgattat gtttggtttg aagtatctga taatggtcct
    12001 ggtatatcaa aggaacttaa aaagcatatc tttgacagat tttttactgg agaagaagga
    12061 gctaaagatt ccagaaaggg tgttggactt ggattatcaa tttgtaaatc tataattcaa
    12121 gctcataaag gtgaaattat ggtagaaaac aataaagata agggttcaac ttttaagttt
    12181 agtttgccta aggaaaatga atagttttaa attttaaaat tagttttgga ggaagaaatg
    12241 agtaaaaaac ttatattgtt agtagaggat gataagacaa taagaaaatt tatatctact
    12301 gcacttttaa cacaggatta tgatgtcaag gaggctataa ctggaaaaga aggtatatct
    12361 atagctgtct catattctcc agatgttgtt cttttagatt taggcttaga agatatggat
    12421 ggaatagagg taataaaagc tatcagacaa ttttcaaata taccaataat tgtagtttca
    12481 gctagagagc aagatagaga caaggtagaa gtatttgatg caggggcaga tgattactta
    12541 acaaaaccat tctccatagt agagttatta gcaagagtaa gagtagcatt tagacattca
    12601 caagtagaag ctcaacaaaa agatgatgtt aaatctacgt ttgaagtaga taaattattg
    12661 attgattttg ataaaagaaa agttattgtt gatgatgttg aagtccattt gacacctatt
    12721 gaatataata ttctttcatt acttgcaaag tatcatggaa aagtgcttac tcataatttt
    12781 ataataaaag aaatatgggg tagtgttata gggaatgaga ctaagtcttt aagggtcttt
    12841 atggcaacgt taagacgaaa aatagaaaaa caacctgcaa acccaagata tataattaca
    12901 gaagttggtg taggatacag attaaatgat gaatagttgt aatatattaa ttattagatt
    12961 atgtgcttat taatatatat taaaattata tagtttagtt aaaataagaa tgaagtgtat
    13021 gtgcttgttg tcagtttagc acatgcattt tattttttag cttttttagt atcaataatt
    13081 ttttagtatc aataatatga aatatgtttt aaaa
//
LOCUS       DAMJZE010000108        13044 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJZE010000108 DAMJZE010000000
VERSION     DAMJZE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.109"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..1176)
                     /locus_tag="PC541_002569"
     CDS             complement(<1..1176)
                     /locus_tag="PC541_002569"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2840022.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     "
     gene            complement(1199..1864)
                     /locus_tag="PC541_002570"
     CDS             complement(1199..1864)
                     /locus_tag="PC541_002570"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2840023.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(1898..3010)
                     /gene="ribD"
                     /locus_tag="PC541_002571"
     CDS             complement(1898..3010)
                     /gene="ribD"
                     /locus_tag="PC541_002571"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2840024.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3142..3263)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3452..5935
                     /gene="recQ"
                     /locus_tag="PC541_002572"
     CDS             3452..5935
                     /gene="recQ"
                     /locus_tag="PC541_002572"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2840025.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6024..6135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6277..7578
                     /gene="thiC"
                     /locus_tag="PC541_002573"
     CDS             6277..7578
                     /gene="thiC"
                     /locus_tag="PC541_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2840026.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7632..7826
                     /gene="thiS"
                     /locus_tag="PC541_002574"
     CDS             7632..7826
                     /gene="thiS"
                     /locus_tag="PC541_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2840027.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7819..8622
                     /gene="thiF"
                     /locus_tag="PC541_002575"
     CDS             7819..8622
                     /gene="thiF"
                     /locus_tag="PC541_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2840028.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8685..9455
                     /locus_tag="PC541_002576"
     CDS             8685..9455
                     /locus_tag="PC541_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2840029.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9472..10575
                     /gene="thiH"
                     /locus_tag="PC541_002577"
     CDS             9472..10575
                     /gene="thiH"
                     /locus_tag="PC541_002577"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2840030.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10576..11265
                     /locus_tag="PC541_002578"
     CDS             10576..11265
                     /locus_tag="PC541_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2840031.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11453..12847
                     /gene="yfcC"
                     /locus_tag="PC541_002579"
     CDS             11453..12847
                     /gene="yfcC"
                     /locus_tag="PC541_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2840032.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         4892 a         1562 c         2222 g         4368 t
ORIGIN      
        1 atgtcccatt ttatcttttt tagtttttaa atatagctta ttaaagtcat taatttcttc
       61 attaataggg attctatttt cgatttttat tccatatttc tctaattgat ttattttatc
      121 tgggttatta cttaaaagat ttatactttt tatgtttaaa ttttttaata tttgagcagc
      181 catatagaaa tctctcatat cttcttcaaa tccaagcatt aaatttgctt caacagtatc
      241 gtaaccttct tcttgaagtt tataagcctt aattttattt aaaagaccaa tacctctacc
      301 ttcttgcttc atataaatta aaactccact tccattttcc acaattttct tcatagaaga
      361 ttctagctgt aatccacaat cacattttag agagtggaac acatctcctg ttaaacattc
      421 agaatgaagt ctaactagag tattttctat atttatattt ccataagtaa gggcaatgtg
      481 ttcttcattt gaataagtat ctctatatcc tatgatttca aaatttccat attttgtcgg
      541 aaggaaagca ctagatactt tttctatagt agtttcattt attttgcggt attctataag
      601 gtcttttata gtgatgattt ttaaattatg cttttctttg aaaatcatta aatcatcaac
      661 tcttgccatt gtaccatcat ctttcattat ttcacagata agaccaatct ctttaaaacc
      721 tgcaagtcta gcaaggtcta ctgaggcttc tgtatgtcca tccctgacaa gaaccccacc
      781 agtttttgct attaaaggaa agatgtgacc aggcgttttg aaatctttac ttgtagaatt
      841 ttcatcacag aataatttta cagtttcagc tctttcatat gcagatatac cagttagagt
      901 atctgctcca tctatagtaa cagtaaaggc tgtttgaaat gtatctgtat tttctctaac
      961 cataagaggt atattaagac ttttaaattt tttttcagtg gcaggtagac aaattagacc
     1021 tctaccatat gtagccataa aatttattga ttcaggagta gccatttcag ctgccatcaa
     1081 taaatctcct tcattctctc tactttcatc atcaaccacg attaccattt tcccattttt
     1141 tatatcttca atagcttctt caatagtatt aaacatgtaa ttcactcctt tattattttt
     1201 aaaaaccatt tttaaataaa aaatctttat ctatatttga tttatggtta ttactattag
     1261 aatttaattc cttgtagtta ttagccataa aattattaac atactttcca attacatcgc
     1321 tttctaaatt aacgaaatct ccaacattct ttgtcaagag aatagtgtta attttagtat
     1381 gaggtataat agaaacctta aatatttcat catctaaata tgatatagtt aaacttacac
     1441 catcaatagc aacagaacct ttgagtatca ttgaggataa aagtttttta tcaggtcgta
     1501 tacttactaa aactgcattt tcatcttttt caaatttgca gatagttcct ttaccatcaa
     1561 catgacctgt tactatatga ccaccaaatc ttgaagaaag actcatagct ctttcaagat
     1621 ttaccttgtc atttgcattt aataaaccta aatttgtgga acgaattgtc tccatcataa
     1681 catcggctgt aaattcattt tttccgaggt ttgaaacagt tagacaaact ccatttacgg
     1741 ctatactatc tccaagattt gtgccatcta atattttatt tgatagtatg gttacttttc
     1801 cactcttacc attagcagta atttttctaa gtattccaac ttcttctact atgccagtaa
     1861 acatcctaac ctcctatatg aatgtattaa caaaaattta atttaaaaca tctgattcta
     1921 taagaatatc gccatctatc aaagttacat tattgattgt taactgtgtt gaatctgaca
     1981 aatgatttat tccttctcca gaaacgaatg taggtgcatc tttaccacct ataatcttgg
     2041 gtgatagaaa taatttgacc ttatcaacta acttatttct aaagagactg tcattgagag
     2101 tagcaccacc ttcaactaga acagaatcaa tgtttaactc tcctaattta tacattacaa
     2161 actccaaatc tactaagttg ttttttgaag gagaaattat tgtttcaaca ttattttctt
     2221 ttaacataga taattttata ctttctttgc aacatgtgaa aactatagtt ttactggttt
     2281 tatctttaac caaattacta ttaagtggca actttaggtg tgtatctatt actattcttg
     2341 taggatgaga cactttttct tgattgagcc tacaagttag taaaggatta tcgttcaaaa
     2401 ctgtatttat gccaaccatt atactttggt atttatttct gtatttatga gttagatacc
     2461 ttgatgattc attagatatc cattttgatt ctagtgattt tgtagcaatt ttaccatcta
     2521 gacttactgc tgattttaca atacaaagag gtcttttgtt ttttatatga taaaaaaatg
     2581 cctcatttaa atctcttgct tcatcttcta atattcccac agtaacatca atattgttat
     2641 cttttaattt ttttactcca ttaccacaaa caagtggatt tgggtctaat gtagatatta
     2701 caactctctt aatcttattt tgtataattt tatctacaca aggaggcgtt ttcccataat
     2761 gagaacatgg ttctaagttt acataaagag tagagtcttt tagactttgt ttagcactgt
     2821 ttatagcatt tacttcagca tgattagagc caaatttctc atgatatcct ttgcctatta
     2881 tattactagc tttaacaatt acacacccaa ctaatggatt tggatttaca aagccttctc
     2941 catttttagc caattcaata gcttttttca tataataaat atctttttct ttttgattca
     3001 caaaattcac aatatgtaca cctcctgttt aattggataa tttttaaaca aaaaaccctg
     3061 agaattaact cagggtttag aataatctac aataaatgtt taataaatgt agaacaaaat
     3121 tgctctacaa aaacactcat taaatatctt cttccatcca gactttactg tcggctttgg
     3181 aatttaacca aatcaaccaa gttttacctt ggctcgcggg ctataccgcc gattgggaat
     3241 ttcaccctac cccgaagatt aagtattaaa tttatattta gaatgtatca taaattattt
     3301 tactttgcaa actatttttt taaaaaatat atgttaatat ataatataga atgtctataa
     3361 actttgaaat accacgattg tgaatataaa aataaaaatt tgaacataat caggaaagaa
     3421 gtctaattaa tttaaattag gagagtaata tatgaataaa aaacttttag aaatactatc
     3481 aaaatactat ggatatacaa gttttagaaa aggtcaagaa tctataataa actctatctt
     3541 aagcgaaaaa gatgtgttag ctattatgcc aacaggaggg ggaaaatcaa tatgttatca
     3601 aattccagct ttgattttag atggaatgac aatagtaatt tctccactta tatcacttat
     3661 gaaagaccaa gtagatgcct taaagactat gggaattagt gctgctttta taaatagttc
     3721 attaagtagc aaagaattta atgaaatatt aaataatatt agaaaaaata attataaaat
     3781 actctatatt gctccagaga gattagatgc acaagagttt attgagctaa taaataataa
     3841 caatgtaagt caagttgcga tagatgaagc acattgtgta tctcaatggg ggcatgattt
     3901 taggttgagt tatagaagga tttctgattt tataaaaaat ttgcctaaaa gacctatagt
     3961 tacagcattt actgcaacag cttcagaaga ggtaagaact gatataataa atttactgtg
     4021 tttagaaaat ccagattatt atataactgg atttgataga gagaatttat caataaatat
     4081 agtaaaatca tctagtaaaa ataagtatat tttagattat attcaaaatc ataaaaatga
     4141 gtctggaatt atatatgttg caactagaaa ggaagttgaa aatatatata atggtttatc
     4201 taagagaaat atatctgtgt caaaatatca tgcaggttta tctaaaaatg aaagaagtaa
     4261 aaatcaagaa gattttataa atgataatgt ggatataatg gtagcaacaa atgcatttgg
     4321 aatgggtatt gataaaccta atataagatg ggttatacat tacaatatgc ctcaaagtat
     4381 agaaaactac tatcaagaaa taggtagagc tggtagggat ggagaaaaaa gtgagtgtgt
     4441 acttttattt tctccaggag atgtacatat acaaaaatat ttaattgata ttggagttga
     4501 aaatcctgaa agaaaattat ttcagcataa gaagcttcaa tacatgattg acttagttta
     4561 cagtaattca tgctatagaa agacaatatt gagttatttt ggagaaaatt atatagacaa
     4621 ttgcaataat tgtagcaatt gtttagtaga gggtgaaata gtagataaaa ctatagatgc
     4681 acaaaaagtc atttcatgtg tagctagaat gaaaagaagt tatggtgtta ctactattgt
     4741 agatgtgctt agaggttcta agaataaaaa gattttacag cttggattaa atactttgtc
     4801 tacatataac attatgagag attattctag tgaagactta aagaattttg taaatacatt
     4861 ggtttctcat ggttttttag atttagttga gactctaggc aatggaaata gaggtagttt
     4921 tcctactata agattaaatg agatgtctat gaaagtttta aaaggtgaag tgaaagtaga
     4981 gtttaaagaa attgttatga caaaatcatt ggaagtagag gatgagttat ataatgcttt
     5041 aagagaactt agacattcaa ttgcaagtga agaaaggata gctccatata tggtatttgg
     5101 agatggtaca cttagagcca tgtctagtag ttatccaatt aataaagaag aaatgctaga
     5161 tatctctgga gtaggagaaa ttaaatatca aaaatatggt agagattttg aagcagtaat
     5221 taaagaatat gtcgaaaaaa ataatataaa taaaaaaata gatgaaaaaa atgtcgattc
     5281 aaagaacgtt aatagtgatt tcttagaggt taatacagat aaagttttat atgaaaaact
     5341 gttagtcatt agagaagaat atgcaacaaa ggaaaaatta cctgcatata tgattttagc
     5401 caataaggct ttgaaagaaa taagtggaag atatccactt gatgaagaac aattaaaaga
     5461 tatttctggc ataggtggag taaaatttga aaaatatgga gctagtattt tggcagaggt
     5521 taataaatat gtaaaagaaa ataatattaa tgttatttgg gagaaaaagg gcaaaaggaa
     5581 gttaatatta gatggagaaa gtagaaaaaa taatgaaata gcattagatt tgctaaatca
     5641 aggaaaagat atacaaagta tcagtcaaga aatagaagtt tcactgtcaa ccattattgg
     5701 atatgtgcat gactatgtaa aggatggaca tagtatatca tttgaattag gactagaaaa
     5761 tttttataat gaagataaaa agaagatgat tttagaagtt tgcgaaaaag ttggctatga
     5821 taatttaaat aacataaaaa gaaaacttcc agattcaata aagtatgaaa atataagagc
     5881 tgtgatactt gactcttatc ttgaaaactt aaaaaataat attatagcag agtaattttt
     5941 aacttaataa aaaataaaat ttgtattatt gtgttgaact aataattttg ctgtgttata
     6001 ctgaatttgt aaaaataatg aaaaattaga gctaggggcg ctagaattgg ctagctgaga
     6061 gataaagccg taacttacaa accctaataa cctgatcaag ataatgcttg cgaagggaag
     6121 ctttttaata taattttatt atataaatag gcaaattatt caatgttagg agaaatttgt
     6181 ctaagtgtat atacgattat aagagtgctt tccaaacgag gaaaagcact ttttttattt
     6241 tcaaaaacta taactttaat cttggaggat tgtaatatga actatacaac acaaatggat
     6301 gctgctagaa aaggcataat aactaaagaa atggaaatcg tttctcaaaa agaacaggta
     6361 gatgtaaatg aattaagaga attaatagca aatggacaag ttgttatacc agctaataaa
     6421 aatcataaat ctttaagtgc agaaggtgta ggtaaaaatt taagaacaaa aataaatgta
     6481 aatctaggaa tttcaagaga ttgtaaggat atagaaaaag aacttgaaaa agttagagta
     6541 gcaatagaca tgaaagctga agctataatg gatttaagta attatggcaa aactagagaa
     6601 tttagagaaa aggttgttga aatgtcacct gcaatgatag gttcagtacc tatgtatgat
     6661 gcagtaggat atttggaaaa agaattaaaa gatataacag aagaagagtt tttaaatgta
     6721 ataagacaac atgctattga tggagtagac tttattacta tacatgcagg tcttacaaga
     6781 agtgtttgcc aaaaaataaa aaatcatgag agattgactc atatagtatc tcgtggaggt
     6841 tctttattgt ttgcatggat ggaattaaat aataaggaaa atcctattta tactaatttt
     6901 gataaaatat tggatatatg tgaagaatat gatgttacat tgagtttagg agatgcttgt
     6961 agaccaggtt gtataaaaga ttctactgat ggtgttcaaa ttcaagaact tgtagtatta
     7021 ggtgaactta ctaaaagagc atgggaaaga aatgtacaag taatgataga aggtcctgga
     7081 cacatggcta ttgatgaaat agaagcaaat gtagtattag aaaagagatt atgccatgga
     7141 gcaccttttt atgttcttgg accattggtt acagatatag caccaggtta tgaccatata
     7201 acaagtgcaa taggaggagc tttagcatgt gccaaaggag tagatttttt atgttatgta
     7261 actccagcag aacatttaag attgcctaat ttagatgata tgaaagaggg tataatagct
     7321 gcaaagatag ctgctcatgc aggagatata gctaaaaatg ttaaaggtgc tcgtgaatgg
     7381 gataataaga tgagtaaagc tagagctgat ttagactggt gtgagatgtt tagacttgca
     7441 atagaccctg aaaaagctaa gagatacaga gatgaatcaa ctcctactca tgaggatagt
     7501 tgtactatgt gtggaaaaat gtgctcaatg agaacagtta agaaaatatt aaacaatgag
     7561 gaacttaatc taatataatt tttggctagt atgactggaa taaaaataag ctgaaaagag
     7621 gaggtgattt agtgaaggtt aatgggaagg aaattgaatt tgaaaaagat ttgactgtta
     7681 tagatttatt aaataaatat aatttaaaaa gtgatagagt tgtagttgaa gtgaatttag
     7741 agataattga agaatctaac tacaatacat atgtattaaa agacgaggac attgtagagc
     7801 ttattagctt tattggaggt ggttaaaata aaaatatttg taaatgaatt attaacagat
     7861 gttgaggaaa atacaactgc ttataaagtt agagatagct ataacaataa atgtgatgta
     7921 ttggtgttaa atggatatcc aattaaaaca gatatgaccc tttgtgaaaa tgataaatta
     7981 acacttatac aaaaaggagt taagccatca cttgatgaat tggaaagctt gttgattgct
     8041 agacatactc caaatataca taataaatta aaaaaaggga aagttgctat attgggattg
     8101 gggggacttg gttccaatat agctatctct ttagctagaa taggagtggg agaactttta
     8161 cttatagatt atgatgttgt agaaccatct aatttaaata gacagcagta ttttatagat
     8221 gatataggaa aactaaaaac tgatgccatg atagaaaata taaaaaaaat aaatccattt
     8281 ataaaattaa ataaacgaga tattttttta aacaagaaca atatggatac tattaaggat
     8341 tcagatttaa taattgaggc ctttgatgat gcttcatgta aagcacaggt atgtaattat
     8401 gtattaataa atttaaaaga taaatattta atagcatcat ctggtatggc aggttactat
     8461 gattcaaata taataactac taagaagata aaagacaggt tttatatatg tggggatttt
     8521 gtgaatgaag ctaaatttgg tgaagggctt atggctccaa gggttgccat ctgcgcaaat
     8581 catatggcaa atttggcaac gcaaatactt atagatatgt aaatattaaa attagatata
     8641 aaaaagattg aatattaaaa aaatttaacg tggagggaaa taatatggat aaattggtac
     8701 ttggtggaca tgaatttaat agcagattat tggttggaac aggtaaatat ggttcaaata
     8761 atatattgcc tgaggtaata aaagaaagtg gaagtgaaat aataacaatg gcacttagaa
     8821 gagtggattt agacaataaa caagaaaaca tattaacgta tattccaaaa gagatgacaa
     8881 tacttccaaa tacatcagga gctactaatg cagaagaagc tgtaagaata gctagaatat
     8941 ctagaaaaat gggatgtgga gattttataa aaatagaagt aatatcagat actagatatt
     9001 tacttcctga taatgaagaa actataaaag ctacaaagat acttgctgat gaggggttta
     9061 tagttcttcc atatatgaca ccagaccttt atgctggaag aagacttata gaagctaatg
     9121 cagcggcagt tatgccactt ggagctccaa ttggttcaaa tagaggtctt caaatgaaag
     9181 aaatgataag aataatgata gatgagttgg atataccaat aatagtagat gcaggtattg
     9241 gaaaaccatc tcaagctatg gaggctatgg aaatgggagc tgatgcagta cttgtcaaca
     9301 ctgctatagc ttcagcagga gaccctgtac aaatggctag agcatttaaa ttagctgtag
     9361 aaggtggaag agaagcatat attgcaaaaa caggaaatgt gtcagaattt gcaaatgcat
     9421 cttctccttt aacaggtttt ttaggcaatt tatagaaagt ggtgattggt gatgagtttt
     9481 tatgatgtaa tagaaaaata tagggatttt gattttgatg gatatttaaa taatgttact
     9541 gataatgatg ttttaagaag tttatcaaaa gacaagcttg aagattttga tatattaaat
     9601 ttgctttcta aaactgcagt aaaacattta gaagatatgg cacaaaaagc acataaatta
     9661 agtgttcaat actttggtaa aacagtatgt ttatatacac caatgtatat agcaaattat
     9721 tgtgttaatc aatgtgttta ttgtagctat aatataaaga gtggaataaa aagaaaaaaa
     9781 ttaactatgg atgaaattag agaagaggga gaagtaattt caaaagaagg tttcaaacat
     9841 ctacttgttt tgactggaga aagtagtttt cactcaagtg tagaatatat aggtgaggct
     9901 atagaaatat taagagagaa atttccctct ataggtatag aagtttatcc aatggaagta
     9961 gaagaatata agtacattgt agataaaggt gttgaaggat taacagtata tcaagaaact
    10021 tatgacgagg aaatatataa gagagtacat ataaaaggcc caaaatcaaa ttataaattt
    10081 agactagatg ctcctgaaag aggggcaaaa gcaggtatga ggactttatc aataggtgca
    10141 cttttagggc taaatgattt tagaaaagaa acttttttta caatattgca tgggaaatac
    10201 ttaaaaacga aatatcctca tattgagctt tcatattcaa caccaagaat gagacctttt
    10261 aaagggtgtt ttgaggaatt ggtagatata agtgatactg atttagttca agctatggta
    10321 tgtatgagat tatttgaccc tcatgcagct ataaatatat ccactagaga aaatttagaa
    10381 atgagaagtc atataatacc tttaggagta actaaattga gtgcaggtgt atctactgat
    10441 gtaggaggtc attctcaaga tgaacatgat actgctcaat ttaagataaa tgatgaaagt
    10501 actgttaaag atgtggagaa aatgctaaac tcaattggtt atcagcatgt gtttaaagat
    10561 tgggaaagat tttaaatgta tctgataaca aacagaaaat tatgtagtga ggaacgatat
    10621 ttagaagtta ttaaagagtc tatattgagt ggtgtagaaa atataataat aagagaaaag
    10681 gatttagaat atcaagagct aagaaaatta tatatgaaaa taaaaactaa aatcaattgt
    10741 atagattttc aagagcaaat atcagatgaa agtttgaaaa ctaatataaa tcaaaaagag
    10801 tttagaaata aattcaaggt taattttata ataaatagta acattgaatt ttttgaaaaa
    10861 gtggattgtc aaggtatcca tcttcctttt aaattattct tgaatttaat tgaaaataag
    10921 tataatttta atgaaaataa aatactagga ttatctttac ataaagtaga ggaagtagat
    10981 tatttagaaa aattaataag aaatcaaaat ataaaaatag attacataac attatcacat
    11041 atatatgaaa ctaaatgtaa agaaggatta aatccaaaag gaattgagct tttaaaagaa
    11101 gctaaaaaga ttactgatat taaaataatt gcacttgggg gaattttacc atctaatgtc
    11161 aaggaaacat taaaatactg tgatgatttt gctataatgt caactataat gaggtctaaa
    11221 gatattaaaa aaaccatatc aaattataat gaaaagttga attagtaaat atagtatatt
    11281 gaatttttaa cttaaataaa aataaaaaag ttatctttta aattttaaaa ggtagctttt
    11341 ttattttata tctttgctaa taaaaaattg taaaaaggga aaactttagt cagagagatt
    11401 aaacaatatc ctagaaagat ttaaaattta gtaaagtatg gaggacatta taatgactga
    11461 cagtaaaaag aaacgattta ttataccaca cacttttaca ataatatttt tactaatagt
    11521 cttgatggca atattgactt ggatagtacc aagtggtgaa tttagtagag aagatgtaga
    11581 aggtagaatg gtagttgtac cagggactta tcaaagtgta gaaagtaatc ctcaaggatt
    11641 tgcagatgtt tttaaagcac caatagaagg ctttatcgat tcagctgaag ttgtaggttt
    11701 tgttttaata gttggaggtg cttttggtat tgtaaatagg actggagcta tagaagcagg
    11761 aatagcttcg gttatctcta agtttaaggg aaaagaactt cttataattc caatatcaat
    11821 gattttattt ggacttggag gtacaacatt tgggatggca gaagagacat taccatttta
    11881 tatgatattt gttccactca tgacatcctt gggatatgat tctctaacag caatagcaat
    11941 tgtatttata ggagcagcag caggagcagc cgcttcaaca ataaatccat tttcagttgg
    12001 tattgcacag gcactagctc aacttgctcc tggttcagga gtaacttaca gagtagttat
    12061 ttttatagca tatatactaa taagtatagc atttgttatg tactatgcta agaaggtaaa
    12121 ggaaaatcct gaaagttcaa tagtatatga aatagataaa aataataaat caatacttaa
    12181 tcaaacttca acagaaataa aagaatttac ttttaaagaa gtatctgttc ttgctatatt
    12241 tggaataggg atggcaataa tgatttgggg tgttttaagt caaggatggt acattccaga
    12301 aatatcaatg tgttttgctg caataggtgt gctttctgga ttggttggta gattatctca
    12361 aattgaaatc tcagaaagtt ttatagatgg agcaaaagat ttagcatctg cagctcttgc
    12421 tatagctttg gctcgtggaa tcgtaataat tgctcaaaat ggatttataa tagatactat
    12481 attaaattcc gcagcagcct ttctaggtgg gcttccaaag gttatatttg tatatttatc
    12541 atttttcttg gaagctgcat tggcattttt aattccatca tcttcaggtc ttgcttcact
    12601 tacagtacct gtacttgctc ctcttggtga tttggttaat atatcggctc aagtgatagt
    12661 aaccgcttat caatttggtg tgggtcttac aaactttata actccaactt caggagtact
    12721 gatgggagca cttgcagtag cccatattcc attttcaaaa tgggtaagat ttataatgcc
    12781 tatcttgttg attttgacag ctgtatctct agtcttttta acaataggta tttatatagg
    12841 gttttaatta agtgtagttt tatttaagat atttagtaat ggtataaaat gataatatgt
    12901 tttcaataaa ttgccactaa tttaattagt ggtaatttgt tttttgtatt taaaaataaa
    12961 tgtatttact tgtattttga attaatatct acttagacat tgttttaatt gttatatttt
    13021 tatttaatta agcattgatt tatc
//
LOCUS       DAMJZE010000109        12916 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJZE010000109 DAMJZE010000000
VERSION     DAMJZE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.110"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(107..1414)
                     /gene="grdB"
                     /locus_tag="PC541_002580"
     CDS             complement(107..1414)
                     /gene="grdB"
                     /locus_tag="PC541_002580"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(365..367),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2840033.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(1500..2786)
                     /locus_tag="PC541_002581"
     CDS             complement(1500..2786)
                     /locus_tag="PC541_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2840034.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(3065..4387)
                     /locus_tag="PC541_002582"
     CDS             complement(3065..4387)
                     /locus_tag="PC541_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2840035.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(4387..6381)
                     /locus_tag="PC541_002583"
     CDS             complement(4387..6381)
                     /locus_tag="PC541_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2840036.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(6468..7715)
                     /locus_tag="PC541_002584"
     CDS             complement(6468..7715)
                     /locus_tag="PC541_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2840037.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            7943..8488
                     /locus_tag="PC541_002585"
     CDS             7943..8488
                     /locus_tag="PC541_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840038.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            8502..9188
                     /locus_tag="PC541_002586"
     CDS             8502..9188
                     /locus_tag="PC541_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2840039.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(9312..9932)
                     /locus_tag="PC541_002587"
     CDS             complement(9312..9932)
                     /locus_tag="PC541_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840040.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9922..10944)
                     /locus_tag="PC541_002588"
     CDS             complement(9922..10944)
                     /locus_tag="PC541_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDF2840041.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(10945..11583)
                     /locus_tag="PC541_002589"
     CDS             complement(10945..11583)
                     /locus_tag="PC541_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2840042.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(11701..12852)
                     /locus_tag="PC541_002590"
     CDS             complement(11701..12852)
                     /locus_tag="PC541_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2840043.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         4157 a         2275 c         1308 g         5176 t
ORIGIN      
        1 aaaatgcaaa tttcgaattt acctaataaa aaaagatatc gtaaaataat tttaaaaaat
       61 tattttttga tatctttttt tatttcagtg ttgtgttgta tttttattat tcaaatactg
      121 tttgttcttc tatatcttct gttagtgcat ttaaagcttt taaaactata ttctttctaa
      181 tttttttact ttcctcttct cctaacatag gatttccaag tggatatggt atccctactg
      241 ctggaacaat tctattagct cctattgtaa gtgatattgg tactactgta catatatgaa
      301 caactgctat accagctctc tcaatctctt ttaccatcgt tgctccacaa cgagtacaag
      361 tacctcatgt agaagttaaa attacagcat ctacatcatc ttctaacaat ttcttagaaa
      421 actcttctcc aaatctctta gaattgccaa cactcgttcc tgttcctgta gtagttataa
      481 aataatctac taattctcca attacacctt ctttttcaag ctctctaact acatctagtg
      541 gtacaactaa atttggattt tcaaggacat atgctctgtc atacccccca tgtatagttg
      601 taaattcttt atttgacata gaatccatat ctgttaaatc gtaaattcca tatttagtag
      661 cacttgaaga ttcaattttg tctggattat caattggaac tatccctcca gaagttacta
      721 gggcaagttt tatcttagat aaatctttaa caggtttcat aggtgtaact ctatcaaatt
      781 caggcattgg atactcagtt tcaaagcttt cccctttcat ctttttaacc aacatatcaa
      841 ttgctctttc agaccctcta agttcatgga agaaattttt tcttattcct ctcattatat
      901 atccttcctc ttcaggaggt cctatttcct cacctctaac caatttaaga cctagtttcg
      961 tcattattgg aactaccttt ctaagacttg ctgctgaatt tcctgtagac actatatgca
     1021 aatctaattt aaacatatct acacctggat tttctttata catgcctgta attacaggaa
     1081 tatttaactc ttcttcaact gccttacaaa tagtcccaca agctactcca tatctacctg
     1141 cattaaatgc tgggcctgca ataaataaat cagggctttc ttcttttatc atttttaaaa
     1201 gttcttgagt tgcactctca atattttcac caaaatacgt atctccgcaa attacagttg
     1261 agattatctc tgcatcatca ttaaattgac tctgaaatgc tattccaggt cctactacac
     1321 cagccctttt ttctggcgga atatctgctt tttcctctcc acctatacca gcaaagaaat
     1381 tgtttatata atgaaccact ctgattttac tcatctctta atcctccaaa tcactttata
     1441 gttattacat cagttagacc aatgattaca tcaacagaac tttatattca aatacttatt
     1501 taataatatc ttgctgtcaa atgattatag ccatttgcta tagttgatgc tattataatt
     1561 tgtagctctg catccatgct tccgtcttca tttaagcatc ctttataccc acctatcatc
     1621 atttcaacat aatctaaagt tcctataatt ttttccattg ctgggaaatg aaccactaga
     1681 tttccttgac cacaagaaac aactgcatct gcttccttag tagcgtctgc tatagattga
     1741 ctcttgccat cttttccagg aaattcatcc gttatcaaaa ctacatttgc tccaacttca
     1801 ctacattttc tacaattcat cattaggtca gtatctggat tcccataacc ttcttctgtc
     1861 actagaactc catcaactcc taaaaattct attaatttag ctaccatatc agagcttctc
     1921 tctttatcga ctaagaatac gttctcatta gtcaaaataa ctcctataaa gtttaaatct
     1981 tttccatgtc ttttatataa gtcatgtata actggattat ttaaatgatg gaatgttgta
     2041 accttatcac atggtgctac acagtttcca gatattatag ctccatccat tatttcagtt
     2101 ggatacataa atgtaggcac tatctgttta gcatccaccc catagtaata agtgtcatgt
     2161 aaaagtcctt gtgattgtaa catgtgaaca tatgctactg ttggcaggtc tggatattct
     2221 tttacttgtt ttaataatgg ctttgtctca tatacaatga cttcatctgg ctcaacttct
     2281 cttcctgcct cacctacata tgcacctatt tttaatcctg caattctagc tgcttcttca
     2341 taatcatgag tatctatacc atcctttgct tttataacaa tacatatatt atttgttttt
     2401 gaaaaaggtg tatatttagc agtaggacca ctcatatcaa ttacgccttc ttgaaatcca
     2461 actatatttc cacaagttac aacacaagct cctaaaagta catgggttct tccacttcct
     2521 acagtcttca ctttatttat aattccagga aatattttgc tttcatctcc aattttaact
     2581 ctaggctcta ttacatcttt aactggagct attcttattg attcgccagg tcttgcaagt
     2641 tcaacattta cacttacaat cctttcatct tctaatacta tcttttctat ttcttcgcta
     2701 ctaatatata gtactccatc ttttacttct gtcttttctc caaattcaac attttttata
     2761 aatatattcc ctagctctag cttcatatga aaatccccct taatatattt aatcgcaatt
     2821 aaaagtattt attacgttta ttaatatata aagcattttc tatgccaatt atttaaaata
     2881 tatattctat gtattaatgt catagaaatt ttcattttta atttacacca atattgaatt
     2941 tagcaataat tattgtaaat attaattttt aaaattataa taaaaaaaga taaacagatt
     3001 ttttctgttt atcttttaat acagtttgag aaattttttt ctcacttaat gagaagtttt
     3061 tttctcactt aatattatac tctgatattt tattggttaa ctgtcttcta ctaatctcca
     3121 attttttagc tgtatttgaa atattatttc cacacaaaga taaaactttt tcaatatact
     3181 cacattcaaa ctcttttctt atttctctta aaggtctaat acttatttta tcatctatgt
     3241 atacattatt tgatattaaa tttaaattgt cttttgataa attaccttct tcacttaaaa
     3301 ctacaagtct atttattata ttttttaatt ctcttatatt tcctggataa ttgtaattta
     3361 ataaaaaatc ctttacttcc ttatcaatcg agtgtatctt tttatcatgt tcaatttggt
     3421 acttatttaa gaaaaattct ataagagtag ttaagtcttc tcttctcttt cttagtggtg
     3481 gtatagttat tgttattgta cttattctat aaaaaaaatc ttctcttata tttccatttg
     3541 atatttccac ttttggttct ttattcatag cacaaattaa cctaaagtca actttaatag
     3601 atttattact tcctatcctc tctatagatt tgtcctcaag tgtcctcaaa agttttacct
     3661 gaacatttag aggtatatct cctatttcat ccaaaaatag agttccttta ttcgaaagtt
     3721 caaatctacc ttttctacta tctactgctc ctgtaaaaga acctttttca tgaccaaaca
     3781 gctcagactc caatagattt tctgaaaaag aacaacaatt tacaggtaca aatatctcat
     3841 tctttcttgg acttatgcta tgtatatatc tagctaatat atcttttcct acaccagatt
     3901 cccccaagat taatatatta acatctttac aagcagcttt ttttgctatt tttatgatat
     3961 tattaaaatc tctattttta gattccaaag taaattcaaa attatttttt tgtacagaaa
     4021 cagatatttt acttgccttt accttttcaa cttcttctaa taaattttca atgggattac
     4081 tttttataaa ataagataaa gctcctcttt tcatagcatc aactgcattt tcaatacttc
     4141 catatcctgt aactaatata acctctatat ctctattaat agatttaatt ctatctaata
     4201 gctgtactcc atccatattt ttcatcataa catcagataa aaccaaatca taatttttat
     4261 cttttttaat tatatttata gcttcttcac cacttagtgc tatatctaca agatagccct
     4321 tcttttgtag aatttttttc attaccactc catattctag ctcatcatca actaatagta
     4381 ttttcattat atagtcacct ctttatttaa tggaattttt atatgaaaac aagttccaac
     4441 ccccaattta ctttcaacac ttatatctcc attattcttt ttaatctcat tataagtaat
     4501 atataaacct aaaccagttc cctcaccaat tggttttgtt gtatagaagg gattaaatat
     4561 gtcctttagg acatcttctt ttattccttc accattatct ttaaaatcta taaataaaca
     4621 atgatttttt tcataacagt atataataat ttttccatct tgatgtattg catctattga
     4681 attagaaatt aaatttataa atacatgttt taacgattct ccatttatat agcatattaa
     4741 attatgttca cagtcaattt ttatttcaac attctttaac tgtaacattt tatattgaag
     4801 tttcactata ttttcaataa aattttttat atttatatgt tctaaattat catctgatat
     4861 tcttgcgaaa tttaacaagt tggttattat attacttgcc ctatctacat tactttctat
     4921 tgatttaaca caatcattta cttcttccat tgtaacgtta tcttttaaga gatagcaatt
     4981 atttcttatt atacccaaag gatttcttat ctcatgtgct accccagaag ctaattgccc
     5041 tatagatatc attttgtttt ctctaagtaa tttcgcctct actattttat cattagttat
     5101 atctttaata agaactacta tatactcagt attatttttt ttatactcta caggaaaagt
     5161 gtttatcttt aaaattttat tgtgtatttc tatttcacta gttttattag tattttcaga
     5221 gaaggtattt tcaataacac cttttatcat gctaatcaaa tctgctatct tataactcat
     5281 ttcacccata aagtcatata gtactttgtt cgattctact atattgttat tttcatctac
     5341 aagcattatt atatctgtaa tactattaaa agttgcctct aaagtttttt tattttctat
     5401 tacctgttct gttttccttt tgatttctat ctttaacaaa tatgtatagc tataaaaaac
     5461 ataaattgat attaaaatta tacctataaa aagataaata ctaggcacaa ttcctcgctc
     5521 atacaatata tcatcaactt cattatacgt agaataccat tttttcttta aatctttata
     5581 cactccattt ttctgaagtt taaaaatgcc tttatttaaa atactaacca gctcttcata
     5641 ttgtttaggt acagcaagaa ctgctttttt agtatatata ggattactta aaacactgta
     5701 tttatttgaa agtccataat tatttatata gtatcgcaat actggttcat caccaacaac
     5761 tgcgtctact tttccagaca ttaaatggtt gactccagtc tttatatctg gagtcaataa
     5821 aatgtttata tcttgaattt tttgctttaa aaaatttata gaataatcac cttctggaat
     5881 tgctactgtt ttccccttta aatccatgta actatttata tcttgggact ttttatcttt
     5941 caatatattt gcactcaaag tgtatattgg gtcagtaaat ataaatttat ttgccctttc
     6001 ttttgatgga atcaagtcaa aaaactttat actatcatct attgaattta cgaaagattc
     6061 tttccaccaa ttatttggtt taaaataaaa atctctacca atttgaattg acagagaatt
     6121 tattaaatca acaataattc ccttatactg tccatcttcc ttacctttat atctcaaagg
     6181 aggtgaactt tggtcagagc tgtaaatcaa attcttgtgt ttttctaacc actttttttc
     6241 ttcttctgta aatggaagtg acttcttaat atattcaaat acatttagat tatactccat
     6301 atttacatac atatttgtaa gtgaaaatac tactaaaaat gaataaataa tccctataat
     6361 aacaatcttt tttttattca ctttaaaact ccaatcttct tttttactat aataagttca
     6421 atatttaata acaaaatcct acttttttca ttaaatattt atatttatta taaaactaat
     6481 tgaatagatg ttgaaattat agtcattact attcctatca atgcttcata tggaattaac
     6541 ttaaatcttt cacctatatc catgtttata ctacctgcac tagtatgaaa tagtgagcca
     6601 tgtggtaatt gctcgaatac actagaccca gcatttacta ttgatgcacc tgaaatcggt
     6661 gatagcccac catttattat agcatcatga aaagtagatg atgctatagt agcaccagct
     6721 gttgatgatg ctgttgctaa tgacataagt atacccgaaa ctggtgccaa taaaaattgt
     6781 ggcatattta aaaattgtaa agcacctatt gtactctgtt gtaattcaga catctgtata
     6841 attcctgcga tagtccctgt tccaagtaat aatatgcata ccccttgcat cttagataat
     6901 ccaaaagtta aatattctct tgaattgttc aagtttcctg tgacaattag agttacgatt
     6961 cctcctattg gaagggctat cattgggtct ataactatag gagatacatt tcctaaaaac
     7021 aatagaaata tagctataat tggaccacat aatgatttaa ataaacttgg taaatcttct
     7081 ctttgctcta aaatttcata tgattgtact ttatttcctt tatttattaa tatacttgct
     7141 aaaataactg taataacaac tccaataatt gctggaatta tatttgccat cataacacta
     7201 gagagattga cagaaaaatt atctgctact gctatagtat ttggatttgg tgatattata
     7261 ttacctgctt taccccctcc caacatagct aatagtattg acaatttaga ataatttaat
     7321 tttctaccta tatataatcc tataggtgct acagttatta tagatacatc caaatttaca
     7381 cctactccag caagtaccat agttgaaatg gcaatcgaaa ataaagccct cttttcacca
     7441 aatattttta caatctgttc tgatatttta tctactgccc ctgtttttat taaacttcca
     7501 gctaatacac ctgatgccaa agctctaaga attgatggtg atatattcat agctccattt
     7561 gccataaaat ctacagtttg tgttatattt gctcctccaa ctaatcctcc aataaatgct
     7621 cctaacatta aactatatac agcttgaaat ttttttataa ttaaaataat tgctattaat
     7681 aaaccaaata tagctccaaa agtcgttatt tgcataatat cccctcctat cataaattaa
     7741 ctatcttttg cttaatttta tttaaatata ttttaagtat tctgtatcca ctttgtcaat
     7801 tatattttaa tttatttaat tatattgaac atagtttttt caaaacctaa ttctatatgt
     7861 tataataatt tcatactaca taatcatttt acttgattat tttaagttaa tattttttaa
     7921 attaaaaaga gaggatattt atatgaatga tttaaattta ggggaaaaaa tagctgaagt
     7981 tcgaaaaaaa caaaacttaa gtattcgaga tttagcaaaa ctagctgatg ttacaccatc
     8041 attattgagc caaatagaac gaggtcttgc taatccatct gtaaattcat taaaatctat
     8101 agcctcttct ctaaatgttc ctttatttac attttttgta tctgaagtag ataaaaaaaa
     8161 cttgatagta agacatgata atagaaaaaa agttatttta cctggaagca aagaagttat
     8221 atatgagatt ttaattccag actctagtgg taatttagaa tttgcaatta tggatttagc
     8281 tccaaacact tcttcttgtg ttgataggat tactcataat ggagatgaaa ttgcatatgt
     8341 tttggaggga gaagttaaac tttttatgga tgatgatgaa tttactttgt ccaaaggaga
     8401 tagtgtaaaa gttcctttag gaacaaaaca taaatggcaa aatgactcaa atagtgaaag
     8461 taaagtaatt tttgcagtca tcttgtaaag gagtaaaaac catgaagaca tataaattta
     8521 tattttttga tgcagatgat actttatttg attttaagaa aagtgaatca catgctttta
     8581 aaaaacttct atctgagttt gatttagaat ttaattttga gaattatata gagtcatata
     8641 gaaatataag tgataagctt tggctcgacc ttgaaaaaaa tattataact ttgaatgaac
     8701 ttaaattact tagatttgaa ctttttgcaa ataaaatatc tttggatgtt gattctgaaa
     8761 ctcttagcaa aatgtactta aattttttag gtgaatgtac atttttaatt ccaggtgcaa
     8821 tcgatatatt acaatatctt aaaaaaaagt atactattgt tataataaca aatggaattt
     8881 ctgcagttca aaataaaaga cttgaaaatt caaaaataaa aggatatatt gatggaatgg
     8941 ttgtatctga agagttgaaa atatcaaaac caaatcctga aatatttaaa tacgctctca
     9001 aaaagtttaa ttgtcatgat aaatcttctg ccttaataat tggggatagc ttaacctctg
     9061 atgtcttagg tggaataaat tcaggaatag atacttgttg gttaaattcc aataacagta
     9121 taaattatac taatcatata ccaacttatg aaataaatac acttattgaa ttaaagaaac
     9181 tactatagtt tgtaagtctg ttcaaattaa atagaatatt actttgcaat aattttttta
     9241 gtttatacat aaaatgacca aaaaaattat tgcaatttat acacaaaaca aatcttagtc
     9301 ttgaaaatta actaaaagca ttttctagga cacttactac atcttgattc taaattttct
     9361 tcatccccca ttaaaataag cattgcaaca ctttttatgg gggatagttg atgataatca
     9421 ttaatttcta tttctatact attatattta ttttttacat agttataaat atccttttgt
     9481 ctctctactt caaaatcttt atctcctgga tatatttcat gcgatatttt gagactatca
     9541 tactttcctt ttaaatacat ttttattttc tcattaatat aatctaaaca tactatacct
     9601 attttatcca atgctagccc tgccatcata ttgcaatttt ccatatagtc attcatctta
     9661 atatcaattt tatcccctat tgtatatagt acagcaaatg cttttttaca ttctttaggt
     9721 gttttattaa tatcaacttc acttatatat atcttgatat ctaaaatatc atttatattt
     9781 cttatttctc tttcaacgat tttactaatt gtatctggag cttttctatt tccatacccc
     9841 aaaaacttta gcacaatatt cttatctata aaaatatcat ctaaatttat atcctttaaa
     9901 agatatttat ttttattact attataattc atcaatatca atcctatcta attctttata
     9961 actaattctt cctgccttta tagctgcttg taaattttct actggagtgg tatttaagca
    10021 gttacagcca aaatcacata aaaagttatc atatcttctc atcttcttta acaattttag
    10081 cgtctctctt ctaatcttat ctggcttaga acgtccaaga agttttattg ggtctaaatt
    10141 accaatcaaa actatatctc taggaatcat tggagcaatt tcttcatagt ctaaaatttg
    10201 gtctaaactt actgcatcta tattacaact tagcatatta tctaataatt ctctggtgtc
    10261 tccacatata tgcatacctt tccaacaatc aagttcattg tagattttat ttaaattttt
    10321 tactataaat tcatcaaatc tatctttact tagggttact gatgttggct ctgctattga
    10381 aatatatttt gctcctgctt tatttgcagc tacagcatat cttcttacca attcagttgt
    10441 aaattccaat aattcttcta caaatttttt atcagttatt atacatctta aaagttgagt
    10501 agctccagct aattgacctg caagtgtaaa tggaccttgt atcgattcat atagtggttt
    10561 tttaatactt tttgatataa ctgataagct ctctatattg agtggcatac gtccactcgt
    10621 ataagggtct ggaacatcta attttcttaa tttatctctg tcagttatag tatgtgtcaa
    10681 tacacttgga aaatcataat ctggtcttaa tgtctctaaa cccagagttt cacaaaatgt
    10741 aatcccatca cagaatgagt atataaaatc tgattcaaaa tacttgtcca tagtctttgc
    10801 taattctaat tgcaaatcta cactatcata tacttcatat gcctttttat ttattaagaa
    10861 caatccattt gtacccatat caggcagaaa aaaacctcta tcttcactat ttatatactg
    10921 aagtaaagtc atatctgaaa acatttaaac ttcacctcct ataagtttat ttattaattt
    10981 aactgctttt ggagcatttg aactgtatcc atctgctcct atttcttctg caaactccat
    11041 acttacaggt cctccaccaa ttattacttt tggtctttta tctatctcaa tcatattaag
    11101 ttcgtctata accgatttca tattttccat tgtagttgtc atcattgaag ataagcctat
    11161 tatatctaca ttgtttttta tagcttcttc tattatatct tcactttttc tatttacacc
    11221 taagtcaata accttatatc cagtggcttc taccattact tttacaatat tttttcctat
    11281 ctcatgagta tctccttcta ctacagacat cagaattact ccctttgatt ttttctttac
    11341 ttttccagtt cccttcaaaa gattaatacc tttatttaat gcatctgtgc atactataac
    11401 ttctgaaaga tagtattcct tattctcaaa cttatctaaa gctcctatca tacctttgtt
    11461 taaaccatat tcataaatat cttccacatc aatattttta tctatagcaa tttttactaa
    11521 attctctata ttatctacac ccatatttaa tatacattct gctatatcat caagtatgtc
    11581 catataatac tcatcctttc taattactta caaaattgct ttttcaatat ccaataattt
    11641 ctcttattta acaaggacta actctaggta tcaatcaatt ttatttaata tcatctaaaa
    11701 ctattgataa tttaaatttg cagtccatgg aaaatctaca ggaaatgcct ttgtagcaaa
    11761 ttcatatata ttttttagat gtttaaattc attgtactgt tcctcatttt tttcaaaaaa
    11821 cagtttatta agcttatcca aattttctat tacaagttct tcttctttgt atttaataat
    11881 atatttattt tcaacttgtt taaattccat gtattttagt aattcaaaat ctacatattg
    11941 actaaaataa cttcttaaac tatcacacaa cttagtaaaa tttattattt tcatagttcc
    12001 ttgttgataa tccaaagatt ttgtcccatt acatttcaac tggtctttaa gatttttcac
    12061 atgtacttta taatttaaat attctaatcc aaatttattt gcaacatatt tcaatatatt
    12121 ttcaacattt acagagttta accctatttc agcaacttct cctacagtag aatcttcttt
    12181 ttttattgtt cttattatca aatatcctat tatattatta ttttccttta taaatacctt
    12241 cttgtaacca attggtcccc aggcaatagt tgctgcatgc aatagttttt gaaactcatc
    12301 tctcgttctt ataaatctag tactattttg attatatagt tcaatcattt tatctaaatc
    12361 attatctttt tcaaaattag tttcatcaaa ttcaactatt tcatatgcta tttttacatc
    12421 ttcaggtttt attgtatatt tataaaaact ctttaccaaa ctacaatttc ttctactgta
    12481 tagacttctt gttcctgata taattacaat atctacacca tcataaaaca ttttttcttc
    12541 tactttatcc aatatcttag atgaatagcc tctcttttca taatctggat gagtacatac
    12601 tccacctata gctgcaacct tcaatctgtt tccttgaata gatacatcct gaattagata
    12661 gtttacatca gaaatcactt tatcatcttc tgatattata atcatatttt ctatattatt
    12721 tttacttagt aaaagaggaa attcttccat catagttggt ttatgatttc ttgatattct
    12781 aaatacatag tttattaatt ctattacttt atcaaattct tctaattttg cacttcttgg
    12841 tcttaattcc attcatattc ctccaaatat tatttaagat acttaaaact ctattatcta
    12901 taataaaaca tttatt
//
LOCUS       DAMJZE010000110        12900 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJZE010000110 DAMJZE010000000
VERSION     DAMJZE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.111"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(73..282)
                     /locus_tag="PC541_002591"
                     /pseudo
     CDS             complement(73..282)
                     /locus_tag="PC541_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(384..932)
                     /locus_tag="PC541_002592"
                     /pseudo
     CDS             complement(384..932)
                     /locus_tag="PC541_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="PC541_002593"
     CDS             complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="PC541_002593"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2840044.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(3480..4265)
                     /locus_tag="PC541_002594"
     CDS             complement(3480..4265)
                     /locus_tag="PC541_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2840045.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="PC541_002595"
     CDS             complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="PC541_002595"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2840046.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="PC541_002596"
     CDS             complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="PC541_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2840047.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(5879..7393)
                     /locus_tag="PC541_002597"
     CDS             complement(5879..7393)
                     /locus_tag="PC541_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2840048.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="PC541_002598"
     CDS             complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="PC541_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2840049.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(8992..10011)
                     /locus_tag="PC541_002599"
     CDS             complement(8992..10011)
                     /locus_tag="PC541_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2840050.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(10187..10726)
                     /locus_tag="PC541_002600"
     CDS             complement(10187..10726)
                     /locus_tag="PC541_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2840051.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(10827..11507)
                     /locus_tag="PC541_002601"
     CDS             complement(10827..11507)
                     /locus_tag="PC541_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2840052.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(11536..12825)
                     /locus_tag="PC541_002602"
     CDS             complement(11536..12825)
                     /locus_tag="PC541_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2840053.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         4155 a         2324 c         1243 g         5178 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac aagtgtgcat
       61 agctattttt taatcaagag aagtctcttc tttcatagac aatattaact ctattctttc
      121 tagtatttta tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa
      181 ccattcctct ggtattatgt tttttctttt ccatctatat aattgtccat aagatatatt
      241 agttttgctc aataaatctt tcttagatat taattcttcc atagctcttc tagcccacct
      301 ttatactgtt ttttacagta gaattatctg aaactgataa attatcactg tattcaatat
      361 ttccaacatc ataattatct actagagata tgcttttttt ctagcatacg aaattttttg
      421 caataatctc catgcccctt ttttatattt atactacttg ctccaacttc attaaaatta
      481 gaagttccat ttaaactaat attaacttct tcacaattca aaaatccatt acactccaag
      541 attccactta aattgatatt ttcacaatct atattctcat ttgcttttaa atatccttct
      601 ccatgaataa tatccgattt tatacttcct tctacattaa taattccact agtatcaatt
      661 tcaacagaat taatatttcc tgtacatcta agaagtcccg atatctcaac ttttgatgcc
      721 agtatgtttc cttctacttt acttaaacca gatgtaaaaa attcattaca ttctgttgtc
      781 ccaaaaactt ttgctgaacc agaagaatca aatttactac attttacact tccttctatt
      841 ttgcctgttc ctgatatatt aacattatca aaaattccac ctttacattt tccaacacca
      901 tcaaatctta gtgaacttaa ttcttcattc ataatgatat cctttcttta ccgtatttta
      961 tctttaaacc taatataaca aaacattgtt acgttgtcaa gtatgataat aatacttaat
     1021 aaagcaacta tcatttattt tctataaata ggcataaaaa taatagggaa atgctttatt
     1081 taaactataa atctaatttc ttagattgtg gtttaaataa aatatatccc tattactcaa
     1141 tgtttatata ttcattgtat aaacttctaa aatataagtc tttaaattct actgtcaata
     1201 ctttttatac actgtttgtg ctatcatctc acacacctaa aggagtaggc tttgcgcact
     1261 attctgtaac ctacaagaca ctatcttctt ctatctattg ataaattttt ctattacatc
     1321 agctagtctc tccatatcag ttggatagac ttcacctatg ctacctattc taaatgtatc
     1381 tatatcagtc aatttacctg gatagataac aaatccatta tcttttaaat aagtataaaa
     1441 ttccataaat tcaaactttg catttttagg atataagaaa gttgttatta caggtgattg
     1501 agcattatca ttcaccaaag tatcaaagcc taaagatttt aatcttgaag ctattgtaaa
     1561 ctgattttct ttatatctag catatctttt ttcaacagaa ccttcttctt ccaattctaa
     1621 taaagcttga taaaaagctc ttacaacatg tgtaggtgaa gtaaatctcc atttaccatt
     1681 atttttctcc atagtctccc attgcgcata cacatcaaga gataatgatt tagctatacc
     1741 tttacattta gataatttct ctttattagc tattataaaa ccaaatccag gtactccttg
     1801 tatacatttg ttagaactac taactaaaaa atctatatta aaatcttcaa catcaatctc
     1861 aattccccca aaactactca tagcatcaac tatataaatt ttgttatatt cttttgcaag
     1921 tttccctacc tcttgtatag gatttaatct acctgtagta gtttcacagt gaaccattga
     1981 tatatgtgtt atatccttgt tttcttttaa taaattttct actacattta aatccactgc
     2041 ttctatatct ttaaatgtgc agtcaacaaa ttgtatgtta agataattac atatatcttt
     2101 cattctcttt ccatatgctc catttgcaat tacaagaagt ttaccatctt ttgaaatagt
     2161 acttcctatt atagcttcta ctgaaaaagt cccacttcct tgcattaata cacttgtgta
     2221 tttatctgta ttttgggttg ctagagatac taatcttctt ctaacatctt gtactaggtt
     2281 attatattca acatcccaag tacaccaatc ttttaacata gatgctctta ctgatttagt
     2341 agtagatagt ggtcctggtg taagtaatat gtaatcattt tctggtacaa aatctgattt
     2401 aatagtttca ttctctattt ttattagtat atcttctaac tcagccatag tttctattac
     2461 aaaatgtgca cctgcctctt taaatttctt tgaaactata ctcatcttag cttttaattc
     2521 ttccttatcc atgttctcta cttcttcttg tgtaagccct aactcacttg aaccttttat
     2581 tacagcaaca ctccacattc ctgcatttac gccttctttt acatcactta ttgtatctcc
     2641 tacttttacc atagaactca taggagacac ccctaaagct tcagcatttt tatagcacat
     2701 ccaaggatac ggtcttcctt gagatacttc tgatggtgtt acaagaaaat cgggtgagta
     2761 tcctttcttt gctgcatttg gttcaactat gttcatcatc tctcttgtat aacccgttgt
     2821 agaacctatt tttaatccat tttttctcaa cttttcaata gtttcaacaa catgaggaat
     2881 tggagtcgta tactcttcta atgtctcaaa taacattggc tcaaattcag cataaagttc
     2941 atttacatca tcttcagttg gcactttacc aaacttatct gaccaaatat tctttattct
     3001 atccatttca cacatttctc ttatatggtc tatttttaat tttcccatag gttttcttgc
     3061 ttcttccatt gttacatcta tacctcttct tttaaatatc tctataaata cattaagagg
     3121 tgcaaaacat ccataatcta ctgttgttcc agcccaatcg aatactactg cttttatttt
     3181 ttctccatat atctttttca tatttatagc ctccatagat tctttttaga atttttgtgt
     3241 ttttaattgt ttttactttt aaagtataac tttcatatgt taattataca tcatatttag
     3301 gttaaatata tattaaattt ctgtttttag ttgtatttag ttgtttttat tttgtatcat
     3361 attcccataa aatcctaagt tacaatctaa aatttacttt ctaatcttaa tctatgaact
     3421 gactatattt tatgaattta gttgtattga gcatagttgt attatataaa actaagggtt
     3481 tattttgctt ctatccatgt tatatttatc tcatttaact tatccttcca ttcattcgga
     3541 ggttctacat cacaaataac aaaatctaat tcctctaaat ctgctatctt atacatacta
     3601 gcaattccta ttttagaact atcacataat acaaaacttt cctttgaatt ttttatatat
     3661 tcttttgaca acaatgcctt attttggtct atactactaa ttccaaaact atcaagtata
     3721 ccttcactag atataaaagc tttatctacg tataagtttt tcatagtatc tattgacatt
     3781 ggacatgctg tcctttggtg ttttaaattt acttttccac caataaatat aacttctcca
     3841 tcaaacaaac cttgattctc ataagaaatc aattttgata gaactggata agagtttgta
     3901 ataattttta aattgtttac agatattata taatctacaa tctgcaaatt ggtagtacct
     3961 tcatctatga taattgactc attgtcattt acaaacttag ctgcatattt agcaatactt
     4021 tgctttttat ctaggttagt attgtttctc tttatatgtt ctggttcaat cactccataa
     4081 tttattttta tagcaccacc atataccttt tttaatttat tttcattttc taattcttcc
     4141 aaatatcttc ttattgtttc tgatgatact tctaatcttt ttactaattc tttagtgttt
     4201 accttccctt ttgaatctaa taatttaagt atttcttttt ttctatctat cgaatttaaa
     4261 gacattcatt ttcctcctaa atataatata tttttctcct ataatattac aatgatacct
     4321 aagatttatg aaatcatatt tatgtattaa attttttact taattaaagt tattttcaaa
     4381 agactttgta ttatcattaa aaaagtccat caaatattag tatcagatgg actttgtaaa
     4441 attttatttt ataaattaat atttaaagtc taactactaa tttattttct tgtctccaag
     4501 aattttacta aattatttac agtagacata tcttttctct ctaaatcagt tggttgaagc
     4561 tctatcccta atctattttc aatttcaagt aaaacttcaa ttatagctaa tgagtctaaa
     4621 agttccgtct caaataaatt aaggtctaaa tcttctctta tctcatcagt ccctaataca
     4681 tcttcaaata tttctataac tgtttcttgc ataaattttc ctccttaaaa ttaagctaaa
     4741 taacctgaga atatcagcaa tccaaaacac acaatatgga atgtaatgaa aatctgcact
     4801 ctctcgaacc atttatcttt tttatgtttt ttataaaatt tagattttcg ctgatatata
     4861 tcagttaaca caagtgccaa tccttggtat acaccataag caacataata ccaagttaga
     4921 ccatgccaaa atcccattgt tatcatggtt atcatctgag ccacatgagc agcagtagtc
     4981 cttttcttaa atcttttctt tctcattgat gacataacaa atctagaaaa aatataatct
     5041 ccaaaccatc ttgaaagact tatatgccat cttgtccaga actctttcat atctttactg
     5101 ataaatggct tgtcaaagtt tataggtact tgtattccaa atatgtatcc tgtacctata
     5161 gcaaataaac tatatcctgc aaaatcaaag aacaggtata aactatatgc gtacatataa
     5221 cttaaaatat ttataaaact catatcttta ggtattcttg atacccaata tgtatttatc
     5281 aaaaatgcta taacaaactt ataacctact cccatgacaa tattttttat tcctggcagt
     5341 aagtattctt ctatatactc ttttctagag atttgttttt ccaagtcctg ttcaaatctt
     5401 cttgacctat caataggtcc agaacttaaa gttggaaaaa ataacataaa ataaagcatc
     5461 tttgaaattt taacttcttt tatagctcca tcataaattt caataaccat ttgtatagtt
     5521 ctgaaattta agtatgatat tccaataaat ccaattattc caaaagaagt aactggagat
     5581 attttattta ttattattgg gagcattgat gcaaacaaga atccccaata tatgtattta
     5641 tttttagttt ttcttcttac atattcatat cctttaacta ttattacttc aagaataata
     5701 aatattacca agtactttag ttggacatca attcctacta ttaagaatat cataaacaaa
     5761 cttgccaaca taccataata ctttatattc ttgcccatta gacctaaaat tactgctggt
     5821 attgatgtta ataataatat ataaagataa aaataatctc catattgtga aaaaatcatt
     5881 aaatttcctc cataagtttc tttctgtcta ttttaccatt gatatttgtt ggaaactcag
     5941 agattattga aatatttctt ggaatcatat atgaaggtat atactttcca agttcctttt
     6001 ttattatcat cccattttta atattactta aatcattctt ttcatttaac tcaacaatac
     6061 cttttaagta agcaattttt tcgtccttat atactggtaa aactactgca ttttttacat
     6121 tatgtacttt tctcaaatta ttttctatat cttctatttc aattctaaat ccatttaatt
     6181 taatctgaaa atcttttctt ccacaataat atatatttcc atctaaaaga tatcccatat
     6241 ctccagtttt ataagctctc cactttacac catctatttc atcatagaaa aatacttcat
     6301 cagtcttttc tttattattg aagtaacctt tagaaactga tggtccaatt attataattt
     6361 cacccttttc attttcttta agctcatttc catcttcatc aagtattttt attttacaat
     6421 ttgacatagg gtatccaact ggaagacttt tttcatcatc tatagccttt tgagtcatat
     6481 catttacact tacaccaaca gtagcctcgg ttggtccata tccattgata attcttgtgt
     6541 ttggaaatct actcataagt tctttagtaa gattttttga aagtgcttct cctatgaata
     6601 tcattgactc aaggttagga agcattttac tattaaactc tttttcagtt atacatactc
     6661 ctgcaaaaga aggagttgac acccatacag ctatatctga gatactaaat tgtctaaata
     6721 gttctttata gtctgctaat acatcttttg atattgaaaa taatgtagct ccatggataa
     6781 gaccagggta tattgttgta actgaaaggt caaaagaata tgctggttgg ttcattatta
     6841 ctttttcact tccatctatg tttagatatg ggcttatcca atctgaaaag ctatctaaat
     6901 tgtttgaact tatttgaacc ccttttggct ttcctgtact tcctgaagta aatagtatat
     6961 atgcattttc atcatctttt acccagtttt ctttatctaa tgattttccc tgatattcat
     7021 ttattatata atttaattta tccatatcaa ttacattgat atctccaaaa tctttttcat
     7081 cactaaagtt aaataatacc tttggtttta tctctttagt gacttcaaaa actctatcaa
     7141 ttggaaaact aatatcaaga ggcacatacg ctctacctga ttttaaagct cctatcatac
     7201 atgccattat catgttttct ttatttccat aaattactat aggagtatct tcttctttgt
     7261 atacatcctt tagaaatact gatatagcat ctgaatattc atttaaatct ttataagaaa
     7321 gtttatctcc attacacatt agtgctgttc tatctgtatt tgaatatttt tttattcctt
     7381 caataatttt catataagaa tgctccttta aaatccgtta taaataaatg gcaattcttt
     7441 aaatggagta aatgttagaa ctcctatcgc caatattata accatagtta aacaaatact
     7501 agttaacaaa tctttattgt tctttaaata ttttaaataa tctctcacag acatctaccc
     7561 atcctttcgt accaagatgc attacatccc ttaaataata cttatctgac cctttatctt
     7621 taaggtttag aacttcaaag cctttgcttt ctgctatttt ttgtgcttta tcgtagtact
     7681 gatttctttc tttttcagaa ataccagtca aattatagaa tttatccata cttgggatta
     7741 atacaacaac tggcttaata cctaagtcag tacatacatt tagcatcagt ttataatttt
     7801 caaactcctt agatgttaat aaatttacat ctttgtctct tcctttgtat tgctctatac
     7861 catctttaaa atgctcttta taatagtact tatcaatatt tagtggattt ttaccaactc
     7921 tttttttagc atcttcaatg gctttctttc tctcatatga ccaatttatt ggtctttttc
     7981 tttttgttgc tctcttttta tctagcttaa ttaatcgttt ataaagaata cccttttctt
     8041 taagagctat acaatacttt cttccttgaa aataaggctc taaaagcacc ttttcagctt
     8101 tttcaggaaa tgtcttaggc tcatatagct tagcatataa tgcttctgct ttatactcgt
     8161 cacttcccca taataactta cttgattttt ttgcatattc aatcttattt tgtttagata
     8221 tttttggatt atctaaaaat cgataaaatt gtattggtga aaatctagac tgataatggt
     8281 gacttgtgac tccgtctttt tccataaacc attgcataga aattaacaag acaactttct
     8341 tattatcaat attaggattc atacttccta atattgcagc atcttgtaat gtctgagtat
     8401 aggctctccc aattgcaaac accttatttt tactcctatt tgtattaaaa tagtactttg
     8461 gatgttgttt tgttgaatta ctaagttctg atgaccccaa aatcataata tccttttctc
     8521 ttaaaaaatg attatttgct gtaacccctt tatcttttat atcacttaaa gtatcatcca
     8581 taattggaag tagatttttc tccctcaaaa gttcatcagt tttagaatca agaaatttgt
     8641 ctaacccaaa taaaaatact acgcctatta taaaaggtgt aataaagtat atcaattttc
     8701 tcatattttc tctcctctaa aaatattcaa atttatgtta ttattaatca tattagtttt
     8761 gatatgtata ttcaactaca aaattgtaat aaaatcgtca aaactttttt tgatactgtt
     8821 acaaaattgt aaaaaaaaag aaaaaaacgc caaaaataaa tttcttttta gcactttttc
     8881 atacttataa tttgacattt taacacttgg ttaagtatac accttacctt aacttaaaat
     8941 taaatttatt taagaatata tattataaat tcttatattt tctaatataa actatatact
     9001 atcagctgga taaacaatac tactttgtct tcttatctca tccataattt ccattactat
     9061 tattgaattt tcatgagaat ttatatttga ttctatttta ttgttattta taagacttat
     9121 aaactctaca agttcataat acattccttc tatttctcta ggcttttcct ctgctttttc
     9181 ttttggaata gatatatctt cttctcttcc atctctgtat ttaataatag ctttttcaaa
     9241 tagattaagc ttttcaatta ttatacttcc ctcttctcct tgtatttcag atggtatata
     9301 agaatcagct actttagaat actgaattat cgcatccata tcatcatatt gaaatacagc
     9361 tgaaccttct ccatccactc cagtttgaag tatatgagaa actgccttta cagtttttgg
     9421 agcaccaaat aaattcacca taggatgtat acaatacact cctatatcca ttaaagctcc
     9481 atttgacaac tcttttttaa aggcattttc tataattcca tttttatatt tatcatatct
     9541 tgaagaatac tgacaatagc ttccaaaaaa tcttcttatt ttccctattt tatataagtt
     9601 ttctttaact gctttaaagt ttggaacaca tgtaattctc atagcttcca taagcaatac
     9661 attattatct ttggctgttt ttatcattga tttgacttct tttaaattag atgctaacga
     9721 cttttcacaa agcacatgtt ttttgtgttt taaacaagta atggtttgct ttgaatgtaa
     9781 tgcatttgga gaagctatgt atacagcatc tatatccttc gatttagcca tttgttctaa
     9841 atcatcaaaa aaaatacttg ctccatatag agttccaaat tctctagctt tttctaaatt
     9901 tctagaatat acagcaacca attcaaagtc tttaacccta gatgcagctc ttagaaatat
     9961 atttgctata ttgcttgtcc caacaatacc aaatcttatt ttacccatca taaatcactc
    10021 cccattttct acactttatt ttaattttaa taaaaaactt tacaacttat agatatatta
    10081 taaaataatt ttatcaaata aaaaagaaag ctaattttaa atgatttcac cattttaaca
    10141 tagctttctt ttaactacaa tctaaattca acttatattt ttaaagttat ctttctacta
    10201 tatctttatt tcccctcaaa aattttatag taagaatttc atccacaaac aatgttacta
    10261 aaagtatagt tacaacaatt ggccaataag gtattataag actatataaa ctatcaacta
    10321 tatacacttg aaaatatcta actccaataa atgtcaatat agtccaatat acagaaaact
    10381 ttagacatac tattcctcta ctattgtact tataatctga ataatcccaa tagaccttat
    10441 taaaatatac tttcataagt attcctgtaa tatattctac tactgtcggc actactagac
    10501 ataataaaag caatgcaaat atattattgg ggtcaataaa tcttgagacc tctattataa
    10561 tcgacattgc aattgcatac attggtttaa atggaccatt taaaaatcca tcttcttgaa
    10621 aatgcccttg tttataataa ctatataagt tttcaattat ccaacctata aaaccataca
    10681 agaaaaaatt aaataaaaaa tataatagag aacctacatt atccatgaat attcctccta
    10741 gctgtttata cttatttttt ctcatattca tggatttatt ctaaatcaaa gaaattatat
    10801 ttaatttatt taattaatac tttatattat agattgtgac attctttaga gttcaaactt
    10861 tttaggtatt ctctaacttc cagtaaattt tcaagtatct tatgtgattg tgatttaatt
    10921 tgctcatctg gctcttttaa atctggtaca tttatacatc ttataccacc attgtatgca
    10981 gcttcaactc ccataggcga atcctctatg acaatacaat tttggggatt aacatttatc
    11041 tttttagctg cttttaagaa tatctcaggg tttggtttag agtttacaac atcatctcca
    11101 caaactatag catcaaagta atcttttaaa tttgccttag ctaatctctt tactgctctt
    11161 tctctctttg ttgatgttgc aacagccatt ttgtatccat tttcctttaa aaaagagatt
    11221 agttcattga ctcctaattt gataggagct cccttcctct ccataaattc aatcatattt
    11281 ttagtttttt cgtcatataa atctattatt ggtacagaac tatcgtatat atctgtaaga
    11341 ccttctatta ttcctttacg atttcttccc ataactgatg tataaatttc cttagtcata
    11401 gtatatccat acttttcaaa cgtctccatc caaaattcaa gagaaattct ctctgagtca
    11461 aacaaaactc catccatgtc aaaaataatt ccttctactt tttgcataca attcctccaa
    11521 tattttttga tataattata ttacactttt tatgattaat tttacaaccc tcaatttatc
    11581 caattctaat attgtaaaat tgtaattttc aaaagttaca ctttggttta ctttaatatt
    11641 tgaaccaagc atataggata tccacccacc tatactgtca aatccatcat gctctatatc
    11701 aagtccaaaa tacttattaa tatagttgat agattcagtt ccatcaacta agtaagaacc
    11761 tgctcctatt ttcttaatat gagtctcttc tttgtcaaat tcatcttgaa tttctccaac
    11821 tatctcttct agtatatctt ctattgttac cacaccagat gtaccaccat attcatccac
    11881 aactatagca agttgaagtt tttcctttct tattctctca agagctttat ctatagttaa
    11941 attttcagat atatatataa tatctctcaa tatttcttct agttcaattt tattttcatt
    12001 tatcttttga ttgtataaat ctcttatatg tacaaaacct aatatatcat ctttattttt
    12061 tctgcatact ggatatcttg taacaccttc ctcttttaaa atagctaata tcttttcttc
    12121 agaatcactt tcatatatac ataccatatc agttctagga accattatct ctctaatttt
    12181 cttttcttca aattcaaaaa tattatcaac taatctttgc tctgattcat ctataagtcc
    12241 acttttataa ctttcttcta ctaatagtct tatttcgtca cctgtatggg gttcatcaac
    12301 ctcatcagct tgtgaatatc caaatggttt aagaaataaa tcagtactta agttgaaaaa
    12361 atatattatt ggatatgtta acttataaaa tcctactaat aaaaaagatg tactcaacat
    12421 tattttttct gtattataaa gtgctaaagc tttaggaacc aactctccta ttacaacctc
    12481 catcattgtt ataattaaaa aagaaatgga aattgatata gtatatataa tactttcact
    12541 aagattaaat acacttacta ttggtaaaat taactcttta actgtagatt ctcccatcca
    12601 acctaatgca agtgaacaaa gtgttatacc aagttgacat gcagaaagat aggaatttaa
    12661 gttgtctttt attattttac atctttttgc attattatta ccttcagtta tcatggtatc
    12721 aatcctagaa ctacgaagtt ttaccatagc aaattcacaa gctacaaaaa aagcatttat
    12781 caaaacaagc attactataa gtgttacttt taataactcc cgcataaagt attggttctt
    12841 ataatgtaag aaatctataa gaactcaaag ttcccccctt atattaataa atttaattta
//
LOCUS       DAMJZE010000111        12878 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJZE010000111 DAMJZE010000000
VERSION     DAMJZE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.112"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     regulatory      103..205
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            299..1675
                     /locus_tag="PC541_002603"
     CDS             299..1675
                     /locus_tag="PC541_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2840054.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1789..2934)
                     /locus_tag="PC541_002604"
     CDS             complement(1789..2934)
                     /locus_tag="PC541_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2840055.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3187..3894
                     /locus_tag="PC541_002605"
     CDS             3187..3894
                     /locus_tag="PC541_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840056.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3941..5536
                     /locus_tag="PC541_002606"
     CDS             3941..5536
                     /locus_tag="PC541_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840057.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5639..6721
                     /locus_tag="PC541_002607"
     CDS             5639..6721
                     /locus_tag="PC541_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2840058.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6870..7031
                     /locus_tag="PC541_002608"
     CDS             6870..7031
                     /locus_tag="PC541_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2840059.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7372..8640
                     /locus_tag="PC541_002609"
     CDS             7372..8640
                     /locus_tag="PC541_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2840060.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8835..9623
                     /locus_tag="PC541_002610"
     CDS             8835..9623
                     /locus_tag="PC541_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2840061.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9623..10072
                     /locus_tag="PC541_002611"
     CDS             9623..10072
                     /locus_tag="PC541_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2840062.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10076..12340
                     /locus_tag="PC541_002612"
     CDS             10076..12340
                     /locus_tag="PC541_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2840063.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12480..12791
                     /locus_tag="PC541_002613"
     CDS             12480..12791
                     /locus_tag="PC541_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840064.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4724 a         1401 c         2190 g         4563 t
ORIGIN      
        1 caatatttaa tataatcgaa tattaataca aaatcaatac aatatatacg tatatattga
       61 caaaattaat tatataaatt aaaataatat tcgtaaagaa aaattcatgt tttgtactgt
      121 ataacatata attttgacaa tatgggtcat aagtttctac cggaataccg taaatattct
      181 gactatgtat actattattt agttaattaa atatttaatt aactgaatta tgaaggatac
      241 ggctcagctt atcaactact gtgtcgtttt tttgtaaaat ttaataggag gtgttgttat
      301 gcaaacaaca aacagtagta ctatgcagaa actttttcca attttgacta ataaaaatgt
      361 cgacatgaag aaggaaataa ttgcaggagt aactactttt ttaactatgg cttatataat
      421 agctgtaaat ccaaatattt tatctgagac aggcatgcct gcaggtgcac ttgttacagg
      481 tacttgttta gcagcagcat ttggatgtat tttaatgggt gtagttgcta accttccttt
      541 tgctttagca tcaggtatgg gtcttaatgc attttttgca tatacagtag ttttacaaat
      601 gggtgttcct tgggaagtag ctcttacagc agtatttgta gaaggtatta tttttatagt
      661 attatcagta agtggagttc gtgaggctgt tgtaaatgcg ataccaaaaa atatgaaatt
      721 agctgttaca ggtggtatag gaatatttat tgcacttata ggtctagtta acagtggaat
      781 tgttattgga gaccaagcca ctcttatcaa aatgggtaga tttacaccag cagtaataat
      841 aacttgtgta ggattaataa ttattgctgt attagataaa aaaagagtta aaggttctat
      901 actttttgga atagtagtga gttcattact tgcttgggca tttgctttta taaatccaga
      961 gtatgcacaa aaattaggta tatatttacc tggtggatta tttaaattcg aaagtttagc
     1021 accaatagca ggtaaaatag atttaggata tgtactacat ccaacaaata taggtggttt
     1081 tttagtagta gtttgtacat ttttatttgt tgattttttt gatacagtag gtacattagt
     1141 tggtgtttgt tctaaagcaa acatgttaga tgaaaaaggg aatgttccta atgtaggtag
     1201 agccttactt acagatgcgg ttgcaactac agttggtgca ggacttggag tatctacagt
     1261 tacaacttat gttgagagtt caacaggagt tatcgcaggt ggaagaactg gatggacagc
     1321 aataacagtt ggaattttat tcttagctgc aatgtttttc tctcctgtat ttatagcaat
     1381 tccatcatgt gctacagcac cagctttaat atatgttgga tatttaatgc ttggtacagt
     1441 taaggatata gagtttgata atataactga aggtgttcca gcttttgtta ctattgcttg
     1501 tatggcatta acatatagta taggtgatgg tttaacttta ggtatattaa cttatgtttt
     1561 cgtaaatata ttttataata tatttggagc taaaaaggca gaagacaaaa aacatgtttc
     1621 tgtagtaatg attgttctgg caatattatt tataataaaa ctttggctac tataataggt
     1681 attatttaaa attttaattt aatataaaat aaagggctgt aatttattat caattgtgat
     1741 gataaactac agctcttttg tatttgttgt atttttgtat ttattgtatt atttaataat
     1801 gtctattagt tctccatttt gtaaagtttt aggtgtaatt actatattga actctttttg
     1861 caaatctttt ataatatcta attctttttt tgttattatt gagattgtgt taccacttcg
     1921 atttcctcta gcagttcgac cacatctatg caggtattca tttttacttt ttggaaaatc
     1981 caagttaaaa acatgactta catccacaat atctagtcca cgagcagata aatcagttgt
     2041 aattaaaatc tttgctttac caagtttaaa tttatttatt gcattctttc tatcttcttt
     2101 ttccatattt ccaaaaattc ctattgcttt gtaattatga taatttagtt ttgatactag
     2161 gacttctata tttttttcat tatttacaaa tacaatagct ttttttggat ttgtagctgc
     2221 taatgccttt ctaagaaaag taaatttatc ccttatctca cctaataaat atgagtgatt
     2281 gatgttagga tttatttgtg atttttcctt tgtttttatt atttcaaact ccttcataag
     2341 tttatcacaa atttttatag tgctatcagt aagacttgca gagcatccta taatttgtct
     2401 atctcttaac gttgttctta ttatgtcttc tatacaagtt atattttttc cattcaatag
     2461 attgtccact tcatctaaga taatagtttt tatattatgt gattttagtt tcttttgttt
     2521 tatcaagtct aaaactcttc cacaagagcc aattactatg tgtggtttta ctgcttttat
     2581 attttttatt tgtttttgta tattgacctc cccaattaaa gctaaagaag taacggagag
     2641 ttctgcattt ttagccaata attcaacctg gtttgtaatc tgcattacta attcatgcgt
     2701 aggtgcaagt ataagagctt gtgtttctct ttttgatgta tcaatttttt caaatatagg
     2761 taaaaggtat gctagtgttt ttcctgttcc agtctgtgaa ttaatcaata aatccttatt
     2821 ttgaattata ttccctataa caagtgactg aacttctgtt ggagaagtta tgtcttgttt
     2881 tttgagtcca tctattagag tggaacttat ttttaattgt tcaaatgtat tcatatttta
     2941 ctccttatat tttaataatt atagtcaact attttttatt atacattgtt tattcaaatc
     3001 gaactatata ttcttttata attatgtgta atttgaaagt aaatatcaat tatatttata
     3061 cctttatatt tctgatacct ttgcatttta tagatttcta aaataaacaa ataaaagttg
     3121 taattgtttg atatttgtta tatattatat ataacaaata ttaaaactat aatatggagg
     3181 gaaattatgt taaaagtaat caatgtatct aaatcatata aaaataaaaa agtagtaagt
     3241 agtgtctcat ttgatgtaaa tgatggagag ctttttgggt ttataggaca taatggagca
     3301 ggtaaaacta ctactattaa agcaattgtt ggaatacacg attttgaaga gggagaggta
     3361 ctaataaatt ctaaatctat aaaaaaacag cctgttgaat gtaaaaagga aatggcatat
     3421 atacctgata atccagattt atacgagtca ttaactggta tacaatacct taattttata
     3481 gctgacatat ttgaagttgc caaagataaa agagaagagt taataaaata ttattcaaat
     3541 aagtttgaac tcaataaagc attaggagat ttaatatctt catattcaca tggtatgaaa
     3601 caaaaattag ctataatttc ggctttgata cacagcccaa aaatcttaat tcttgatgaa
     3661 ccttttgtag gtcttgaccc aaaagcttcc tttatattga aggaaattat gagggagttt
     3721 tgtgacaaag gtggttgtat atttttctca acacacgttt tagaagttgc tgaaaaaatt
     3781 tgtgataaga ttgccataat aaaagatgga aatataattg cttatggaac tacagaagaa
     3841 gttaagggaa gtaattcact tgaaagtatc tttatggaat taatagagaa ataaaatgtt
     3901 ttttatacaa tatagtgtct aaagtgagga atgataaaat atgagcaatc taagtatatt
     3961 aataaaaact aatataataa atgaatttaa attaaatgcg ttaaaaaaag ctgatggtag
     4021 agaaaaacaa aaaataatgg gaatgatgct ttcagttgtt ataatagcgg gtctattaat
     4081 tttttatgta acaagcttaa gtctctcttt agttgatatc ttaaagcaaa taaatcaact
     4141 ggaaatgcta ctgatatttg gttttggatt atcaacatta acaactattg ttacatcatt
     4201 atataaaacg tcatcatatc tatttcaagc aaaagattta gaccttttga ctagtttacc
     4261 tataaaagaa tctactgtac tagcaagtaa gatattaatg ttggttggag caaactattt
     4321 attttcttca atatgtatga ttatacctgc agtggtttac tttttaaatt ctgatgtaag
     4381 tataacttat ttcccttatt tgatattgct atttttagct ttaccattat taccgatagt
     4441 tgcttcatca ataatttttc tatttatagg gcagatatca tcaagaataa aatacaaaaa
     4501 tttagtatta atcataggta gcattatatt gatgttgctt tataccctac ttatgtcaag
     4561 acttaattct atgtcagttg aaatgctaaa gaatagtgtg tctatttcag agacaattag
     4621 aaaagtatat cctcctatat attattttgt tgatgctctt aaaacaggaa gtatagtttc
     4681 atttttagca tttatagcca tctcaataat accatttttt atgtttgtaa ctctatttgc
     4741 aaaaaaattt aaagatataa atgctaagat gagtgaagga tataagtcaa aaaattatga
     4801 atttaagggt caaaaaactt caagaccttc aaaagcttta ttaaaaaaag aatttaagag
     4861 atatacttct tcgtttatat actttttaaa ttcatctgca ggtttgttat ttctaatggt
     4921 aggtacaatt ttaatattat tatttggagc agataaagtg ggtgagttac tgaaattgaa
     4981 tatagatttt aatcttataa aaatacaatt attgggagta ttcttcttta taatagtaat
     5041 gaattgtaca acatactgtt caatttctct tgaaggaaaa aacttatgga tactgaaatc
     5101 ttctccaata gatgaaagag aaatattctg gtcaaaaata ttgatgaatt ttttattgaa
     5161 tgcaccacta tctattttat gtctaatatt agtatctata agattagact ttgaaattca
     5221 gtttattatt gtatcaatct gtttgattgt aagtttttca atgtttgtag cattgatggg
     5281 attattatca aatttattat acccaaacct tgattggaaa aatgaggtgg ctgtagtaaa
     5341 aagaagtgca agtatgatag ttacaatgtt agtatcatta gcttatgtaa ttatacttgg
     5401 aggtatatat gtattacttg gaatttcgtt tttaaatata tatattattt tggcaagtat
     5461 acttactttg atattagatt ttatgatatg gagaattatt tctactaaag gagtaagaat
     5521 atttaaaaat ttataggtgt atttatgtca aataaagact tatctttttt agtcttgcaa
     5581 ataataggtt aagtagtata ctatgtatag tgatatgaaa aaatttagga gaaaataaat
     5641 gagaaaatta tccaaaagag aaaaagattt tattcaatat ggatttttat tatttttgat
     5701 atgtattact acttatttag tattaactac tttagatata aaactaatac ctaaaattat
     5761 aaggctagta aataagactt atatatttct tggcatattg acaattatag tatatatgat
     5821 aattgaatct attgtaactc atttgataat aaatgcagtt caaaaaacaa aagtaaaatt
     5881 tattggtatt aaaatggcta caatgggatt ttactataac ttagttactc catttgcatc
     5941 tgggagtcaa ccaatgcaaa tatatgcact tacaaaatat gatgtaagcc ttagtaaatc
     6001 agtggctata gttactaata aaactgtagt atttcaaagt acagttactg tattttgtgg
     6061 aattttaatt ttgctaaata agtcattact gatggagcaa gtacattctg tgaaagtctt
     6121 gataactttt ggaatgatta tgaatgtaag tatgttgtta ggtggactac taatagtatt
     6181 tagtccaaaa caagtaaaaa tgatagcaga agttttgatt aattttttat ctaaatttaa
     6241 aacatttaaa tttttagatt caaaacgtga tagaataaat cattatattg atgattataa
     6301 ttattctata aaaatattta ttaaaaatgt aagaatctta gttttaagta tattacttac
     6361 tgtgttacaa ttaatcgcat atttcagtat agtttattgt gtgtataaag cctcaaattt
     6421 aagaggagtt tcatatgtac atataatgac attacaggtc tttttatata tggctatatc
     6481 accaattcca acaccaggaa atgttggagc taatgaggtt acatttttta ctatgttctc
     6541 caatataatt ccaaaagaat tattggggta ctctgtactc ttatatagtg ggtttgtata
     6601 ctattttata ttaatagttt caggtttatt tacatttgga acgcattata cattaaattc
     6661 ttggaaaaag gaaaataaag taaattctaa tttagataag gttattgaaa aattaaatta
     6721 ataaaataat tagggaaatt ctatatataa atatggtgtt gaataagcta tattatattg
     6781 agatatttcc cttttttgat taaaattata gaaatttgaa ataataagta ataaacagaa
     6841 aaagtgatat tgttttgaaa ggagaaacta tgaaacaaga taaacaaaat gaaaaacaaa
     6901 gtgagatttc taagataaaa caaagcaatg ataataaact tgaaatagct caagaattac
     6961 atgtaagtaa aaatataggg aatatgcttg gatatagaat agataagaaa aaaatgaata
     7021 gaccaagtta atgaaaatgt tgaaaatggt acattttaaa aaatatatat aatgaaatgt
     7081 tttattgtta aatttaaact tattttaatg ggggaaaaag tcgaaaaata tctaaaaaga
     7141 tatggatata aaaaatatta atatatatat actataaact tgacaaaatc aaccttttaa
     7201 taactagcaa aaaatagcaa aattatataa gcgttattta attgtcaaaa atgaaagcta
     7261 aattgcatgt cctaaaaaaa atgattgata acatttattt gtgtgctata attatatagt
     7321 aaaacaataa gtatattgca taatgtatgc aaatcgggag gagagacgga catggaagca
     7381 tctatacaca caattgccag caataattta ttgataattt ttgctattgt aagtataact
     7441 ggaataattt gtagtaagct tagtgaaata ataaaggttc cagatgtagt gttatattta
     7501 cttgtaggta ttttaatagg accatcattt ttaaaattta tagatattcg tggatttcaa
     7561 atagaaaatc aactaatact tacatttggt tctgcattta ttttatattt aggtggaaaa
     7621 gaaataagtt taaaagtatt gagaaatgtg aagatatctg tattcttatt agctacttta
     7681 ggagttgtga tatctgcttt tataatgcaa caagtaatag gatttacttt tgggataagt
     7741 gcaatgactg ctttactagc aggaactata atagcatcaa cagacccagc cactcttgta
     7801 cctatattta atcaggtaaa gataaaggat agggtaaaac aaactgtaat aagtgaatca
     7861 gcttttaatg atgcaacagg agctatacta acatcagcag tgttggctgt aattttgtca
     7921 aataaatttt cattaggtga aaatatttat gaactgggga caatggttat tgtcggagtt
     7981 ttagttggtt taattacagg aatactgctt ttgaaattag ttaatgataa accttatgga
     8041 atatttaaag attttgcacc aataatatct gttatttcag ttgtagttgc atatgaatta
     8101 tctacaaaat taggtggaag tggatatatg tcttgcttta ttgtaggtat aataactggt
     8161 aacaagaaaa attttaaaat ttggttaagt caaaaatctt atgatgcaga tttttatgtt
     8221 gctgaaactt taggtactat ttgtcgtatg gcaatattta taatattggg tagccaagtt
     8281 aatttggctc agttaagtaa atattttatg ccatctgtaa ttacagtatt agtattaatg
     8341 tttatagcaa gaccactttg cgttttagtt tgtacacttg tagacaggga agcaaaatgg
     8401 agtaaaagtg aaatattatt tatgatgtgg gtaagggaaa caggcgtaat accagcagca
     8461 ctttgtggta ttatttcagc aatgaaagtt cctggatacg aagtgatttc atctgtagta
     8521 ttcatgacta tactaattac attagtaata caaggaagta ctacaaaatt agtagctaaa
     8581 aaacttggat tattagaaga agaagtgata aatataagtg aaaaagtatc aactttttaa
     8641 agttttgctt taaaatagta cctgttaatg gctattttaa tattgtctaa tgatgattat
     8701 tgaaatagtc atttttttat gtataagtaa tttacagaat ttacatatta gacatattta
     8761 ttatcaaaat ttgttgaagc tatgatataa ttagttgtat ttaaaataag aatagcaaat
     8821 aagggaggaa atttatgttt acggttatgg atattgtaca gccagataca gtagaagaag
     8881 cgtactcaat tttaaataaa agaaaaacta accaagtaat tggtggaagt gcttttttaa
     8941 gaatggggaa aaaaagaata ggaacaggaa tagaattatc taaattaaat ttagattata
     9001 taaaagagta tgaagattac gttgagattg gttctatgac tacttttaga acattagaaa
     9061 caagttccat aataaaaaat aattttggaa gaataataga agattcagtt aaaaatataa
     9121 taggagttca gtttagaaat gtagtaacag ttggtgcaac agtattttct aaatatggtt
     9181 tttctgactt gatagtagca ttactttcat tagatacgga agtagaatta tataatattg
     9241 ggagaattag tcttgaagag tttctaaata gagattatga gaaagattta cttattaaga
     9301 tatacataaa gaagacaaat aagaatgctt cttataaatc aattagaaat gcaaagagtg
     9361 attatccaat tctaaatgta tctgtatcaa aacatatgga acaatttaag ttatgtgtag
     9421 gagcaagacc gcagaaagct acgattgcaa aacaagctag tgaatttcta tctaacaatg
     9481 aaatcaatga aattaatata gataaagcag tagaaatagc atcagaagaa ctaacttttg
     9541 gttctaacat gagagcatct agggagtata gaaaagctat gagtaaagta cttttaaaaa
     9601 gagctataat ggaggttatt taatgttagt aaagattaat ataaatggga agaaaaaaag
     9661 tgttgatata gaaccagaag aatatctggt tgatacttta agaaaagttg gtaatttaag
     9721 tgtgaaaaga ggttgtgaca caggttgttg tggtttatgt acagttttaa ttgacaagaa
     9781 acctacatta tcttgtgcaa cactgacatt gagagcttta gataaagaaa taactacaat
     9841 tgaaggttta gaaaatgaag ttaaagaatt tagtgaagtt ttggtaaaag aaggagctga
     9901 gcaatgtggt ttctgttctc ctggctttat acttactgta cttgcaatga aggatgagct
     9961 tgaaaatcct acagatgatg aaataaagca ttatcttaca ggaaatctat gtagatgtac
    10021 agggtatatg ggacagttaa gagctatcaa aacttatctg gaggtaaaat agaacatgaa
    10081 tatagttgga aaaggaatca agaaaataga tggaatgagt atcacaacag gaaagcctgt
    10141 atatacagat gatttatcag caaaagatgc tttggtggta aagatactta gaagtcctca
    10201 cgcttatgca aaaataaaag atatagatac atcaaaggca gaaatggttg atggtgttga
    10261 atgtgtttta acatataaag atgtaccaaa atctcgattt acacttgctg gtcaaacata
    10321 tccagagcca tctccatatg atagattaat tcttgaagat gttgtaagat atgtaggtga
    10381 cgaagtagct atagtggcag ctatagatga aaaaactgca attaaagcaa tgaatcttat
    10441 caaagtaaag tatgaagtac ttacacctgt tttggatttt gaagaggcaa taggaaataa
    10501 aactatggta catgatgaag atagacattg taactttgat ataggtatgc aaagagaaaa
    10561 aaatatagta tcccatcatg attatacaaa aggtgatgta gaaaaagtat taagtaattg
    10621 tgatgtagtg attgaagaaa cttattatac tcaagctcaa gcacatgcaa tgatggagac
    10681 gtatagggca tacaattatt tagaccatat gggaagattg gttgtagtaa gttcaactca
    10741 aataccattt catgtaaaaa gacatttatc tcgtgcacta gaaataccag ctagcaaaat
    10801 tagggttata aaacctagaa ttggtggcgg atttggtggt aaacaaactg catgtgttga
    10861 gattttttct gcaattgtta ctttaaaaac agggaaacca gctaaaataa tttatgatag
    10921 aaaagaaact tttaattgtt caacaagtag acatgcaatg agattaaaag ttagactagg
    10981 tgctacaaat gatggcataa taaaggcaat agatataaaa gcactttctg atacaggagc
    11041 atatggagag catgcttcta caactattgg tttggttgga gaaaaaactt taccaatgta
    11101 taataaagtt gaagcaacaa gatttattgc agatgtggta tatactaata agatgccagc
    11161 aggagcactt agagggtatg gagcatcaca aggatgtttt gctgttgaat caactataaa
    11221 tatgcttgct aataaattaa aaatggaccc aacagattta aggttaaaaa atgtggtaac
    11281 agaaggtgaa actacatttg catatggtaa aactttaaat agtgtcgatt taataaaatg
    11341 tataaataaa ggcaaagaac ttattggatg gaatgaaaaa tatccttgta aacatcttga
    11401 taatggaaaa attagaagtg tgggtatggc aattactatg caaggttctg gtatacaagg
    11461 tcttgataag tcttcagctg aaataaaatt gaatgactgt ggagattata ctcttatgat
    11521 aggttctact gatatgggta caggaagtga tacaatttta gctcaaatgg ctgctgaggt
    11581 tcttgataca agcattgaaa atataacagt tatatcagct gatactgact tagttccata
    11641 tgacccaggt tcatatgctt caagtactac ttatgtaaca ggtatggcag tagttaaggc
    11701 agctcaagac ttaagagaaa aaataatttc tgctggagca actaaactag gtgttgagaa
    11761 ggatgaagta gaatttgatg gtataaaatt aactagtaaa aaaggtagca ctttattatt
    11821 cgatatagca gttgattcta caattggtcc agatactaat caattaatag gtcttgcttc
    11881 tcatggtagc ccagtatctc ctccaccttt tatagcaggt tttgcagaaa ctgaaattga
    11941 cacagaaact ggaaaagttg atttagtaaa ttatgtagct gtagtagatt gtggaacggt
    12001 tataaataaa aatctagcta agatacaagt tgaaggtgga attgttcaag ggattggaat
    12061 ggcattattt gaagaagtta gatacacaga aaaaggaagc atggacacaa atacatttat
    12121 gcaatacaag attccagcta gatgtgatat tggaaatgtt aaagtagatt ttgttgaaac
    12181 ttatgaacca actggaccat ttggtgctaa atctgttgga gaagttgtta taaatacacc
    12241 tttaccagca atacaagaag ctgtgtttaa tggaattggt gtaagaataa atacactccc
    12301 tattacacct gaaaaagttt ttatggaaat gaataaataa ttatttatga aaatttagac
    12361 tagttttaca tgttaaacaa ccagttatgt catacctgtt gaaagatatt taataagtag
    12421 ttattataat attatgaatt ttacttataa aatacaaaat taaataaggg gatgatgttt
    12481 tggctggtaa tctaaataat atgagagcag taaataattt tagaggagat aagaacattt
    12541 tagaatgttt agtcagcttt gagggtcgtt caataagtca gagaaaagta agggtatttt
    12601 ttaaagaaaa acaaaatcaa atagaaattg attttgcaga agaggaaatt tctaaattgg
    12661 ttgaaaatgt tgttttaaat acatcatatc aagaaatgtt atatgatgaa atagagaaac
    12721 aactggaaat tgattgtata ggtacttgga tgatattatc taaattaaaa gatggtagta
    12781 gagttcactg attttaaaat aaattattag agcctaattt taaggctctt ttttgctgtt
    12841 tttaattaac aatagaaaat ttatctttag tttgtata
//
LOCUS       DAMJZE010000112        12802 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJZE010000112 DAMJZE010000000
VERSION     DAMJZE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.113"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            134..1648
                     /gene="glpK"
                     /locus_tag="PC541_002614"
     CDS             134..1648
                     /gene="glpK"
                     /locus_tag="PC541_002614"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2840065.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(1762..2391)
                     /locus_tag="PC541_002615"
     CDS             complement(1762..2391)
                     /locus_tag="PC541_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2840066.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            2979..3770
                     /locus_tag="PC541_002616"
     CDS             2979..3770
                     /locus_tag="PC541_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2840067.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            3799..5019
                     /locus_tag="PC541_002617"
     CDS             3799..5019
                     /locus_tag="PC541_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2840068.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            5039..5734
                     /gene="pxpB"
                     /locus_tag="PC541_002618"
     CDS             5039..5734
                     /gene="pxpB"
                     /locus_tag="PC541_002618"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2840069.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            5764..6750
                     /locus_tag="PC541_002619"
     CDS             5764..6750
                     /locus_tag="PC541_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2840070.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(6964..7530)
                     /locus_tag="PC541_002620"
     CDS             complement(6964..7530)
                     /locus_tag="PC541_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840071.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            7882..8949
                     /locus_tag="PC541_002621"
     CDS             7882..8949
                     /locus_tag="PC541_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2840072.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            8968..10128
                     /locus_tag="PC541_002622"
     CDS             8968..10128
                     /locus_tag="PC541_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2840073.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            10141..12237
                     /locus_tag="PC541_002623"
     CDS             10141..12237
                     /locus_tag="PC541_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2840074.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(12396..12740)
                     /locus_tag="PC541_002624"
     CDS             complement(12396..12740)
                     /locus_tag="PC541_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2840075.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
BASE COUNT         4884 a         1458 c         2346 g         4114 t
ORIGIN      
        1 agaaaataag tgtcttctta ctaaatatct attatattaa atttgtactt aagtatgatt
       61 atttattgaa atatgtgata ttaaattttt ctttttaata aattaaatat tatatgataa
      121 ggagaatatg gtgatggaaa agaagtatgt aatggcatta gaccaaggaa caaccagttc
      181 aagagctata ctatttaata aaaaaggcga aatagtgaaa attgcacaaa aagagttcaa
      241 tcaaatttat ccaaaagctg gatgggtaga gcatgaccct atggaaatat gggggtcaca
      301 aagtggggta atgagagaag ttatagaaac agcaggtata agaccagaag aaattgcttc
      361 aatagggata actaatcaaa gagagactac ggttgtttgg agtaggtata ctggtaaacc
      421 tatttataac gcaatagtat ggcagtgtag gagaacgtca gaaatatgtg atgaacttaa
      481 aaataaagga ttggaagaat caataaaaga aaaaacaggt cttttaatag atgcatactt
      541 ttcagcaaca aaagtaaaat ggatattaga taatgtagaa ggagctaggg aaaaagcaga
      601 acaaggagag ttattatttg gaactataga tacatggtta atatggaatt taactcgtgg
      661 aaaagtacat gtaacagatt actcaaatgc atctagaaca atgatgtaca atataaatga
      721 tttagaatgg gatgaggata tattacgaga attggacata ccaataagca tgttgccatt
      781 agtaaaacct tctagctatg tttatggtca tacagatgag cgcatgttat caggagctaa
      841 aatacctatt gcgggatgtg ctggagacca acaagcagca ttatttggtc aaaattgtgt
      901 tgaagaaggt actgcaaaaa atacttatgg aacgggttgt ttcttgctta tgaatacagg
      961 cagtaacata gttaaatcta agcatgggtt acttacaaca attgcttggg gagttgatgg
     1021 aaaagtaaca tatgcattag aaggaagtat atttattgga ggagcatctg ttcaatggct
     1081 tagagatgaa ttaaagataa ttgaatctgc aaaagatagt gaaatgtatg caaatagggt
     1141 agaggatact aatggtgtat atgtagtgcc agcatttaca ggtcttggag ctccatattg
     1201 ggatatgtat gctaggggtt caattttagg acttacaaga ggagcaaaaa aagaacatat
     1261 agtaagagcc acattggaat caattgctta tcagacaaag gatgttttag aagcaatgca
     1321 aaacgattca aaattaaaac taaaatcatt aaaagttgat ggtggagcaa gtaataataa
     1381 ttttttaatg caatttcaat cagatatatt aaatgtagat atagatagac ccaaaatagt
     1441 agaaactaca gctttaggag ctgcttattt agcagggttg tctgttggat tctatattgg
     1501 aagaaatgaa attacttcaa agtggtcagt agaaaaagag tttaatccaa atatgagtga
     1561 agagaagaga tgtaaattat ataaaggatg gaaaaaagct gtatcgagag ctttaagctg
     1621 ggaaaaagaa gatgaattgg atatttaaaa attaatcaaa gttaaattta tttgaattta
     1681 aaaagagctt tatgtaaaat atgaagctct ttttttgttg ttaatcttgc atttttatat
     1741 aatcacacta aattatataa tttatttttc gttttcatat aaaaacattg gtatagaatt
     1801 gtaaatgtgt tggtcaccaa ctactaagag aatgtctcct tctaaaaatt caatatatgg
     1861 tccaggagat attataagtt cttcacctct tttaattgca actattgtag cacctgtatt
     1921 ttgccaaaat ttagtttgtg cagctgtttg accaatatat ttgcaattag aatttatggt
     1981 gaactgaacg ggcactaaag gattgattat ttcaagtctg ttagaatagt ctaaaatttt
     2041 atttatagta tctaatattt cattttctaa attagattta gtatctatta aattataaag
     2101 tgttgattta tatgatgata tatttgtaat atctctatat ttttttataa aagaagaagc
     2161 tttgtctgga gatagtacaa taacaccttt accctttatg cttttaacaa cgccaatgtc
     2221 ctctaaaatt ttgacagctc ttcttatcgt ttctggagat acattgtatt taccagccaa
     2281 aacagagcgt ccaaatagta ctgaatcctc ttgtatttca cccgtgtata ttttatttgc
     2341 aatatccaac gctatttttt ggtatattgg catcgtatag tttttatcca ttgtttttcc
     2401 cccaatacaa aatttatgtt aaaaaaatat ataatcttaa caatactatt gtataattta
     2461 taggtttata tgtctacaat atgtacaaat attgtgcatg ttttaaaata atattttaac
     2521 atgcatttag tatacatgat tttattatac ttaaaactat ataaagaatt aattataaaa
     2581 atataaagat attttactta caaagaatga taattatata gatgaaagaa acaaatgaat
     2641 gaagaaaaat gattctgtct tgcatataaa gtgattaaaa atgaaaaata ctttaaaaat
     2701 gaaggttaaa gcccttattt taagtgaaaa taattaatta ttaactaaaa aatggtaaaa
     2761 agattttttg aaaaagaaat acaaatgaat gttcaattaa aataattgca aaaatattcc
     2821 ttaaatggta aaattatatt aattaaatgc aatagaatag ttgcaaaaat aaaattgaat
     2881 acaagtgata aaatatttca tgcaatatat aaaataaaca aaatttaaaa attaaaacta
     2941 cttattctat caaaatgtta gcgaggaggg gtcataaatt gtataaagta gatttaaata
     3001 gtgatttagg agaaagtttt ggaacgtata agattggttt agatgaagaa gttttaaaat
     3061 atatttcatc agccaatata gcttgtgggt ttcatgcagg tgaccctagt catatggaaa
     3121 aaacagtaca gctagctaaa aaaaatggag tgaaaatagg cgcacatcca ggtttcctag
     3181 atttaatagg atttggaaga cgtgagatga aaataacaaa gcaggaagct aaagattata
     3241 caaaatatca actgggtgct ttaatggcat ttgcaagttc aaatggatgt aatatacaac
     3301 atgtaaaacc acatggagca ttatacaata tggcagctaa agataaagag ttagctatgg
     3361 ggatttgtga agctatatat gaagtcgata aagatattat actgttgggg ctatataata
     3421 gtgaaatgat aaattctgct aaagaaattg gacttaggtt tgcaaatgaa gtatttgcag
     3481 atagagctta tgataataat ggttttttgg ttccaagaaa tgtagaagga gcagttatac
     3541 atgatacaaa acatgcaata gatagggttg ttaggatggt aaaagaagga actgtagaaa
     3601 ctttaacagg tgaagttata catatcaaag cagattctat atgtgtacat ggggacaatc
     3661 caaaagcaat agagtttgtc aaagaaataa gaaagcgttt tgaattagaa ggtatagaag
     3721 tttgtccatt agaaaacata gaagtttgtt cgtcagagaa catagtataa gactgataac
     3781 atgaggggga gataaaatat gagtacaaaa aatattacag ataaaactaa aaataaaaaa
     3841 gatgtcggag cacttatagg ggctgcattt ataatggcaa catcagcaat tggaccaggg
     3901 tttttaactc agactgcaca atttacccaa gattttggac cgaatttttc atttgtaatt
     3961 ttaattacaa caatcctttt tataggggct caagtaaatg tatggagagt aattggtgta
     4021 tcaggactta gaggtcagga tatagcaaat aaaataatac ctggattagg ctatttagta
     4081 gcttttttag tagctcttgg aggattagct tttaatatag gaaatgttgg tggagctgca
     4141 cttggtatga atgtaatgtt taatatgaat atgacattag gaactgtatt aagtggatta
     4201 atagctatat ttgtatttat gtcaaaaaat tcaaattctc tagtcgataa gataactaaa
     4261 tttttagcat taggaatgat aattatagtt ggatatgtag caatatcaaa tcatcctcct
     4321 gttggagagg cagtatcaag aatggtaaaa ccagaaaatc caaaaggact gatattccca
     4381 ataataactt tacttggagg aagtgttgga ggttatataa cttttgctgg aggacataga
     4441 ttaatagacg gtggaataac aggagaagaa aatataaaag aaataactaa atcttctctt
     4501 ttgggtatat tagttgccac aatgatgaga gttcttttat ttttagcaat actggcagtg
     4561 gtttcaaaag gtcttcaact tgacccagaa aatccagcag cttcagcttt taaatttagt
     4621 gcaggagcaa taggatataa attctttgga cttgtattat ggtctgctgc aattacatca
     4681 gttataggag ctgcttatac atcagtttct ttcttaaaga cactaaatcc ttttatagat
     4741 aaatatgaaa aatattttat aatagcattt atagcaatat caacacttat aatggcattt
     4801 ataggaaaac cagctacttt attaatttta gcaggagctt taaatggatt gatacttcca
     4861 atcacattag gaattatgct tatagcatct aaaagaaaag atattgttgg agattacaag
     4921 catcctacat ggcttttaat atttggatta atagtagttt taatatctgc atatacagga
     4981 ataacttctc ttagcagcct aggtgcttta tttgcttaat ttagaagagg tgacatttat
     5041 ggaaactaga tacttgatat caggggataa ggcagtggtt gctgaatttg gaaatgagat
     5101 atcagaagat ataaacaaaa aagtaatttc atttatgaga gcaatagaaa tatcgaatct
     5161 taaaggtatt gtaacagaaa tggtaccaac atataggtca cttatgataa gttataatcc
     5221 attagaaata gattttgata gtttgattga gaatttaaaa aaaatagaag ataatttgga
     5281 aagtatagta ttaccaaaac ctaaaataca tgagattcca gtatgttatg atgaggtttt
     5341 tggtatagat attaaaaacg ttgcaagcta caataatctc actgtagatg aagttataaa
     5401 aatacacaca agcagagagt acttgatata tatgctaggg tttactcctg gatttccata
     5461 tctaggtggt atggatgaaa gaatagctac acctagatta gaagtgccaa gaactaaaat
     5521 ttatggtggg agtgttggaa tagcaggttc ccagactggt gtatatccta ttgatagtcc
     5581 tggtggatgg caaataattg gtaggacacc tttaaaatta tatgatgaaa atagagaaga
     5641 acagattttg cttagagctg gagactttat aaaatttgtt ccaataacat tagatgaatt
     5701 tatagaaatc gaaaaaaata acttaagtat ttaaatatcg ttatagtaca gggagtggta
     5761 tatatgggat tcaaagttga attaggaggt ttccaaacat taatacaaga cagaggtaga
     5821 gttggctatg gtcaatatgg tgtatctgga tgtggagcaa tggatgagta tgcacataga
     5881 gtaggaaata tattagtagg aaattctgaa gatgaggctt ctttagaagt tttaatgtta
     5941 ggtcctacaa ttacctttga tgaatatacg caaatagcag taactggggg agatttaggg
     6001 gctaaaataa atggaaaaga aattcaaaac tggaggtcat atcaaataaa tccaggtgat
     6061 gtattatcat ttagaggtgt gaaatcaggt actagagcct atgtgtcaat agctggtgga
     6121 atagatgttc cacttgcaat gggaagtaag tcaacttata caagagctaa aattggtgga
     6181 tttgaaggta gagcacttaa aaaaggcgat tatataaata cttttattca ggaaaaagat
     6241 tttacaataa ataaaaaatt aagttcaaaa tatattccaa cttatagttc agaaatcgta
     6301 ctaagaattg taaaaggtcc tcaatttgat gctttttcta acggtgaagt agagaaattt
     6361 ttatcaaata aatacaaagt tacgaatgaa atagatagaa tgggttgtag actagatgga
     6421 gagagtataa agcaccttaa tggagcagat attatatctg agggtatttc ttatggagct
     6481 atacaagtac ctggtcatgg aaaacctatt ataatgttat cagatagaca gactagtggt
     6541 ggctatacta agatagggaa cgttataagt gtagatttgt ataagttagc acaagcaaag
     6601 ccaaatgatg tagtaaaatt tgaacttgta gatatatatg aagcacatag actattgaga
     6661 gagcaagaag ataagattca agatatctac aagagtatga aaaatataag agttgtaaag
     6721 gcaaaagtat taaatgatat agcagtttaa ttttaacaaa tacaattttt attatagttt
     6781 ataatataac ttacaaatag ctatttacta tttaccataa ttaagatggt ttagattagt
     6841 caaatagcta tttttttagt ttgagtaaat attttaaatt taaataaata cttcaatttt
     6901 aaaataaaag caatatttaa atctattata ttatctacaa aaagtaggtg tgtcatattg
     6961 agtttattta gattttattg gccaaattgg aggtgatata actccatatc cacgagctat
     7021 tgtatcttta cttggattga tgattttatg tggtacatta gcttttataa atgttgagtc
     7081 acctggattt aatctaacta ccttttcacc catacaaata tcaaccacac cactcgttac
     7141 caccataatt tcttctgatg catgatatat ataatctttg gcatcctcac tttctggttt
     7201 aagttcaaac tctacaaata aaattgaagg tgtaaactca gttgtaggca ttggagatac
     7261 tatctcgtaa aacatttcac tctttggaaa tttcatcata actctatctt cttttctaat
     7321 gactaaatca tcagtattat tgtcatccat aaacatatat aaaggagtgt ctaaaacttc
     7381 agcgattttt ctcaatgaag acaaggatgg ttcaatagca tctctttcta tttgagagat
     7441 ataacttata gataaatctg tcaatttgga aacatcactt aatgtttttt tcttgctttt
     7501 tcttatattt ctcatctttt cacctagcat aagaacctcc aaataataaa attaatataa
     7561 aatattcgct taaaacaaag taattatagc acaacataga agtagtggca ataagaatac
     7621 atagtagatt ttgacaatta aaatttaatt aaatccaaat aaaagtctat gaaaatgaat
     7681 tattgttaaa ttaatacaaa gaaaataata ataaaaaaat ttaatagaag agtattaatt
     7741 gaattaagat tatcacatta tattttggaa aagagtaaat tttcagtaaa ttatcttgac
     7801 atatatattg aagtaataat ataatttaaa gtataaaatt ttaaaaattg aattataact
     7861 tcaaaaaata aggagggtaa tatgaagata acagatatca aatttgaaaa gcttagaatt
     7921 aaattaaaaa aacctgtagt agtttcattt ggagtaattg aatatggaga aagtataatt
     7981 ttaaaaattg aaactgatga agggtattgt ggatttggcg aagcagcgcc tcttgcagca
     8041 gttacaggtg aagttttgga taatgttctt tcagtattgc ttatgtttaa aaaagagtta
     8101 ataggcaaag acccactaga tattgaaacc atacatacaa tcatggatgg aattattata
     8161 ggtaatactt ctgcaaaagc agctattgac atagctttat atgatattaa aggaaaaatt
     8221 atgaatgcac ccctttataa agttcttgga ggatttgata gtaaagtcca aacggatatt
     8281 actataagta tagataagcc agaaaaaatg gcagtagaag ctttagaaag agtaaaagaa
     8341 ggttttagga tattgaagct taaagcaggt ataaatcctg aagacgatat tgaagctgta
     8401 aagttaatta gagaagccgt tggagagagt ataagaatta gaattgatgc aaatcaagga
     8461 tggaacgtaa atagcagtat aaatactatc aaaaagttag aagaatttaa tgttgatgca
     8521 attgaacaag cacttccaca ttgggattta gatggtactg cttacataag aaataaaagt
     8581 aatacaaaaa ttatgataga tgaatcatta cattcaccaa tagatgcaat taaggctata
     8641 aaaaagaatg cagttgatac atttaatatt aagctgatga aatcaagtgg aatatatcct
     8701 gctataaaaa tcaacaacat agctgaggca agtggagtaa attgtatgtt aggatgtatg
     8761 ctggaaacga gaattggtat aacagcagca gcaaatttaa tagcatctaa aaagaacata
     8821 acagaagctg accttgatag ttttatgttt tgtgaagaat tagaaggtat atctggagga
     8881 tttgtaatgg atggagatat tatgaatctt gtcaataaac caggtttagg aatagaagta
     8941 aacttatgat aaatttggag gttaaatatg aatacacttt tttcaaatcc aggtagtata
     9001 cttgctgtaa tgacatcaat gattgcattg ggtttttact tacaaagata caaagccatt
     9061 aaaagcttag gaccagcact tacaataatc ataatgggaa taatactttc aaacttaaaa
     9121 gtagttccag taagtactga gttatatgga actatttcaa catatgctat tccagtttct
     9181 atgactataa tgttgatgag tgttgactta aaagaaatga caaaattatc aagagaacct
     9241 ttaattgcaa tttttgtagc tgtattaact gtaagtatca tggcattttt atttggatta
     9301 gtatttgcag aaaaaatatc agaaggttgg aaggttgctg gaatgtttgt aggtacatat
     9361 actggtggaa gtgctaatct tacagctatt ggaacaggtc ttaatgtaag tagacaaact
     9421 cttgctgcag caaatgcagc agactatgta attggagtac caacattgat atttatgttt
     9481 gctctaccag caatattaaa aaattcaaag aagtttaaaa aactttggcc atatcatgta
     9541 gaagaatcag aattagagga ttgtcaaaat gaagaattta tggagtcaaa agaatggagt
     9601 attaaagata tagcttggat gttggctata ggttttgttg ttacagaagt tgcaacaata
     9661 cttgctggat atttcaattc gagttttagt agtgcagcaa gaatactttt agttacaaca
     9721 atttcaatta taattgctca attaaagcca gtaaagaaac taaagggaaa tttagattta
     9781 ggattatttg ttgcattatt ctttttatgt acaataggtt tttcagtaga tataaaagaa
     9841 tttttaggtt caacttttac tataacactt tactgtttta gcataatttt tgcttcattt
     9901 gttttccatt taggcataac tagactttta aagataaagt atcaatatgt aatactttct
     9961 atagtaggag caattgctga tggtcctact tcagctttag tagctgcttc tgcaaagtgg
    10021 aattcgcttg taagtgtagc agtcgtaatg ggagtaattg gtggagtatt aggaaattat
    10081 gcaggtatat cagtggcata tgcaataaag atgtctctag gattataggg gggagtttaa
    10141 ttgataaaat ttaaattata catatctgga ctatacagtg gaaatgtaat ctttgatgga
    10201 gatttattaa ttgaaaagtt gaatcctttt acaaataaaa tagaaagttt aaaaccaata
    10261 agcaaggaag aaaatacata ctatttaaat cttacaaaaa tagatttaaa gtctttattt
    10321 aataattttg atgtatatac taaaagctta gtaaacacag ataaagacac agttataaat
    10381 aacttaggag aaacgcattc taaacttaat gaatatatat gggttcaaag aaacaaaaag
    10441 ttcccactag atataattat tgtagataat aaaatagtag gttttatctg tttatccaga
    10501 gaaacttgta ctatattaat tatggatgga tatgaagagt atactgtatt aaaagaatgg
    10561 gaaaaaactc ataaaaatga agagatatat tctataagat ttggtggaaa ttatatgatt
    10621 gatatgaaag atggtataaa actttctaca gatgtatatc tccctgattt tgtagattct
    10681 accaaaaaag caccgacaat tcttatgaga actccatatg gaaaagaaaa tgataaagaa
    10741 atatattata aatatgtaca aagaggatat gctgttgtaa tacaagatgt taggggaaga
    10801 aatgaatcag aaggtaagtg ggaacctatg attcatgaga gggaagatgg agatagtacc
    10861 ataaattgga tagtatcaca ggaatggtca agtggaatag ttggaatgct tggagcatca
    10921 tatcttggtt atgtccaatg ggcagctgca tcttcaggca ataagcatct taaagcttta
    10981 gttagtatag taacatcagg aagccctttt attgatatac caagaaaagg aggagctttt
    11041 gtatctggta tgcttgcatg ggcatttatg gtatcaagaa ataaagttga tagaagcaaa
    11101 atggtaagag atgattggga tgatgtttta aacatacgac caatagaaaa tatacctgtt
    11161 gaagctcttg gatatagaat tgaatttctg gaagaatggt taaagagagt agaaaaagat
    11221 gagtattggg atttgatgga ttggcattta caaaaagata aaataaatgt acctgcattg
    11281 gttgtatctg ggtggtatga tgataatagt atgggaacta cagaggcctt agatgttata
    11341 aaggactatg aaaaaggaaa gagaaaggct attctaggtc catggatgca taattcaaat
    11401 actttaagag atataaatgg tatttcttta ggaaatagtt cattaagata tgatttggat
    11461 tataattatc tattatggtt tgataaatac ttaaaaggta tagaaaataa catagatacg
    11521 actgctccag tagagtatta ctctgtaggt tctaataagt ggaagacaga agaaaattgg
    11581 ccaataataa ataagataga taaaagtatg tatctaataa gtgatggaaa tgctaacaca
    11641 tctttaggaa atggtagatt agtatttgat aatggtttag aagaaaagta tgatagttat
    11701 atatataatc caaaagaccc atcagtacaa ctaattgata tgtcagagaa tgaagttgga
    11761 gttccaaata actacaaaga tttagaaaaa agaagtgata tgttgtgtta tacatcagat
    11821 gcttttagtg aagaatttac tgtaacaggt gatataaagt tagagttttt tgcatcaagt
    11881 tctgccaaag atactgactg ggttataaaa attatggacg tggatttaga tggaaattca
    11941 attaaattag ctgatggtat acttagtgct agatttagaa atagttttta taagtcagag
    12001 tttatggaag aaggagagat ttataagttt acgattataa cttcaaaaat atcaaacacg
    12061 tttaaagttg gtcataaaat tagactagat ataacgtcta gtgctaaaaa ctttattttc
    12121 caaaatagca acacgacaga gggatataat agtattgaat atatagaagc aaagaatacg
    12181 atttatcatg gaggtaaata tccatctaaa ttgatattgc caatagagaa taagtaaatt
    12241 tatatagatt aaatagtgag gaggtgtctc aaaatgaatt ttttagttcg tgagacactt
    12301 tcttttatac aaaatcaaat gtattttcat catttcaaca atgaaaaaga ggcttatctc
    12361 tctgttttga gatagcctcc tataagctaa attgattatt ttgtagctgc aacaacgctc
    12421 atttcaacta acaaatgctc tctagcaagt ctagcttcaa cacaagctct agcaggttca
    12481 aatccttttt caacccaagc atcccaaaca gagttcatag cttcaaagtc tttcatatct
    12541 cttaaataaa tagtaacaga tagcaaatgt tgcttatctg aaccatattt gtttaataaa
    12601 tcttctattt tagctaaaac ttcagttgtt tgttcaatta ctcctccttc agcatgagtt
    12661 tgtccacata aataaactgt attgttatga actacagctc tactcattct tccagttcct
    12721 tcatatctct ttatttccat attttaaata cctccatagt taaaaaatct attaaaaatc
    12781 taaataataa attaaaaaaa gt
//
LOCUS       DAMJZE010000113        12794 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJZE010000113 DAMJZE010000000
VERSION     DAMJZE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.114"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            120..308
                     /locus_tag="PC541_002625"
     CDS             120..308
                     /locus_tag="PC541_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2840076.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(468..620)
                     /locus_tag="PC541_002626"
     CDS             complement(468..620)
                     /locus_tag="PC541_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2840077.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(718..1194)
                     /locus_tag="PC541_002627"
     CDS             complement(718..1194)
                     /locus_tag="PC541_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2840078.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1413..2174
                     /locus_tag="PC541_002628"
     CDS             1413..2174
                     /locus_tag="PC541_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2840079.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2434..4362)
                     /locus_tag="PC541_002629"
     CDS             complement(2434..4362)
                     /locus_tag="PC541_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2840080.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4596..5048
                     /locus_tag="PC541_002630"
     CDS             4596..5048
                     /locus_tag="PC541_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2840081.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5051..5359
                     /locus_tag="PC541_002631"
     CDS             5051..5359
                     /locus_tag="PC541_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2840082.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5473..6579
                     /locus_tag="PC541_002632"
     CDS             5473..6579
                     /locus_tag="PC541_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2840083.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6654..9332
                     /gene="mngB"
                     /locus_tag="PC541_002633"
     CDS             6654..9332
                     /gene="mngB"
                     /locus_tag="PC541_002633"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2840084.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9649..11982)
                     /locus_tag="PC541_002634"
     CDS             complement(9649..11982)
                     /locus_tag="PC541_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2840085.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11957..12673)
                     /locus_tag="PC541_002635"
     CDS             complement(11957..12673)
                     /locus_tag="PC541_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840086.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4769 a         1783 c         1669 g         4573 t
ORIGIN      
        1 gaaattcttc aatatgtgta aggcaaatat atatttctat tgcaaatttt tgttatttat
       61 gataatataa atatgttttt agtctttaaa tagttatcat tttaaggagg tgtgcgtaga
      121 tggcaaaagt atgtagcgta tgtggtaaag gtaaggtttc tggaaaccaa gtatcacact
      181 caaataaaca taataaaaga acatggtcag ctaacttaag aagcgtaaga gcgattatag
      241 atggagctcc taagagagta aaagtttgta ctagatgttt acgttctggt aaaattgaaa
      301 gagcttagta accacgttta aaagcctact tccgtaagta ggctttttaa tttaatcatt
      361 tttactcttt ctcataaatt ttaggattat acttgaaatc cattttggta attttattgt
      421 tactactttc atcaatactc accccacact ttatagttaa aactatttta acaaaaaaat
      481 aataatgctc cacatccaat aagtattaat gcggtaagtg ctgaccaaat ccagccagga
      541 agcaacattg acagaattac agccattcct gttgctagac aaagtattcc tagcaatacc
      601 ttagacttgt tatttctcat ggaacacctc cacatagaac ctgtatctat aaatattttc
      661 atattcaagt ttatgcgaca gttttccatt ttagaccaat aaacacatta tgtaatttta
      721 atttttcata ctagaattat tactgaactc cgatatagat tctacaatat ccaaaattcc
      781 tttttttcta cttaaccttt caggtccact atataaaaca actggtataa ctttaggaag
      841 tttaaaattt ttatcttcta tttcttcaag gctttttttc ataataaaac ttttccaaat
      901 ttgttctcta tacgatttca atcttgggac aatttcagta tccatttctg tttgaaattc
      961 taacactata taatgtgata ctttgccgct tttagaaaat accttataaa ttatatcaac
     1021 ttcttttcct ttatattcac atataaaaga tgtttctaac tctatatttt taagagttaa
     1081 ttcttttgct attgagacac ttgtaaaatt ttgaatcaaa cttaaaaaac tttctttatc
     1141 tgaatacatt cttctgtact cttctcgttt ttcattgctt ctttttaaat tcatacagtt
     1201 cctcctagac tatagacctt ctatttttta gtaaagatag tctataaatt ttatttttta
     1261 attttcacta gtgagtattg acagttgtgt gtaaaatatc cagtaaagat atttttttat
     1321 atttttatta aaagatataa atttttatat tttaatgtat tgacatttac gttgcgtaaa
     1381 agtgtatatt taatttatag gaggtactac ttatggaata tacagttcaa aaattaagta
     1441 aactagctgg tataagtact agaacactta gatattatga tgaaataggt cttcttaagc
     1501 cacttaaaat aaattcttca ggttatcgta tatatggtca aaatgaagtt aataaacttc
     1561 aacaaatact tttttataga gaattaggaa ttaacttaga aaatataaaa aacattataa
     1621 attcaccaaa cttcgatagt ttaagtgcgt taaaagaaca ccataataaa ttactagcca
     1681 aaagaaagca aattgattta ctaatagaaa atgtaactaa aactatagct ctgaaggagg
     1741 gaaaatacac tatgacagat atagaaaaat ttgaaggttt taaagaaaag atgattgatg
     1801 aaaatgaaaa aaattatgga actgaaatta gagagaaata tggtaaagat attatagata
     1861 catcaaataa aaaacttaaa aatatgtcta agcaagatta tgaaaattgg caaaacctaa
     1921 atatcgaaat aattagtaaa cttaaaaaag cttttaaaac tggtgatgcc tctagtgaac
     1981 tggctcagga agttgcaaaa ttacatcata aatggttgtg ttacacttgg aacacatact
     2041 ctaaagaagc tcattcctct ctagcacaga tgtatgtata tgatgaacgt tttacatcat
     2101 attatgatag agaacaacct ggattagcta agtttttaag ggatgctata gtaatatata
     2161 caagagataa ataatataaa ataataaagg tggtgtctca aaatgaactt tttagttcat
     2221 gagacactct tttttgtatg aaatcagata tatttttatc attttatcaa tgaaaaacag
     2281 gtttctctct ctattttgag acagaatctt tattgtataa taattataat caatacaaaa
     2341 taataacaaa ttatacaaaa cttacttata ctatttatgc taaaaacaaa aaatatttta
     2401 ttaaagtttt taaaacttat gtaatataaa aatctatact atagacttga ttatggaaag
     2461 aaaattatca aaatcattac aattagatat actatctcta atatcattgt catgaattat
     2521 ctttaaaagg atatcataaa tggagtttat ttttatataa tcatctttat ttatagccaa
     2581 catcatgatt aatttaacag tagaattttc actccaatat attggttcat tcaatatgga
     2641 aatacaaatc ttagttctta tagagcttaa ttccatagga tgaggtatag ctaaaaaatc
     2701 atctatattg gtagaagata aactttctct gtaaaataca gattcagcaa atgatggaaa
     2761 tacaatttca ctttgagtta atttgttgca catcaacttt ataagctcat ctttatcttt
     2821 gattttagga ttaacaataa ataaattttt atcaaagaaa ttgtccaaaa gattactata
     2881 aatatgggaa ttattagtga ttgacttaga tatattctct atatcttttt ttaatagttt
     2941 taaatccact aatacaatag gaatactatc atgatttaaa ggaattgtcg atattattat
     3001 atctacatct gacaattcac tagctaaaaa ttgattaaat gatagacatt gaagtatatt
     3061 tattttatca tgaaaaactt tgtttaattg aacctctaat aacctacttg aaccataccc
     3121 tgaaccacat actaatacta cgtttttaca cttaatatta ctttgaaaaa atctctctat
     3181 tccagcacct atatgcaggg atacatatcc aatttcatct tctgttaaag agtaaatact
     3241 atttttaaaa actttttcta tagcatttaa agttatttca aatgctaaag gataattact
     3301 ctttattgta tttataaggg gattcacctt atttaaatta taagatttag aattgagaat
     3361 tgatttaaag tgcagtacta aatcatcaaa taatacacta tcatttctaa ggtcaaaagt
     3421 atattgattg ttgattactt ctaaaatttc ttcaacatac tctataattt tagtatctac
     3481 cctattggaa acgttatcca aaagagaact tttagttata aaatgattat atagataaac
     3541 tctgtctgcc tttgaaataa ttatagtata tttactctca atataattaa atatattttc
     3601 aacagttgta ttcgattgaa aatctgttaa aacaacatct aaaacattat ctaagcagta
     3661 accatcaagt attctactta cagtaatagc taaatggata ataaagtttt ttagattaaa
     3721 atctaggaaa ttgatattaa atttattaat ttcacttaac actacccttt gaagctctat
     3781 taaatctaca ttattaaata attctatttc tctatctgta attccaatta tatagttttc
     3841 aaaattccta tttatcatat tattagaaat acatcttcga atatctattt ccttaccttc
     3901 aactttaact ccataatatg gcttagaaac taatattaaa ttatactttg acaaaatagg
     3961 ctttattctt tttatgtcat ttagtattgt aaatttactc acatacaagc tattagctaa
     4021 atcttctagt tttatatatg tattgttata gagaatatat tttaaaatat attgatttct
     4081 ttctatagga gaatctggta tttcacttga atccataata tcatcagata ttaatgctag
     4141 atactgttgg tataatgcta aattaagaat atctatataa tatccttctc ctttttttat
     4201 tttaataata gcaccatttt tctctaaagt tccatttata gaagtaatat catttcttat
     4261 agttctctca ctcactttta aatgattact taaagtttta caagtaattt tatcatcact
     4321 ttcatttaat aatttaaaca acgtgagcaa tctcttgtgc ataaatatcc ccccttatag
     4381 aatttcttct aaattatact atttacactc atataataca attactctaa taaaattgta
     4441 ttataataat ttatttccac ttctatatgg aaattagtta ttatcataat ataaatttcc
     4501 atgtcgaagt ggaaattagt tatatttaat ttaattagga taatgattac aatataaata
     4561 taagttaaga cattatgaat ggagtggaaa aatatatggt actacaagaa aaatatatat
     4621 ttatagataa agacttaaaa agcaaagaag aagtattaaa ttttattgca gatagtgctt
     4681 atatactagg tattacaact aataaagagg ctcttttaaa agaccttcta aaaagagaaa
     4741 atgaatttcc aacaggaata aaagatggat ttgcgatacc tcatacaaag agtgtaaatg
     4801 taaaggaagc ttccatactc tatattaaaa caactaacga gttagaatgg aaaactttag
     4861 atgatagtaa ggttagatat gtatttagtt tgttagcccc acataaaaat gaaggaaata
     4921 tacatttgaa aactctatct aaattagcca catgtttaat ggaggatgat tttacaagtg
     4981 aagttaaaaa tgctagtgat gcaagtcatt tagtaaaata tataactaat aaaatgaagg
     5041 aggaataatt atgaaaatag tagctataac atcatgtata gcaggattag cacacacacc
     5101 tatggcagca aaagctttag aacaagctgc atcaaaaatg ggtcatgaaa tcaaagtaga
     5161 acaacaaggt gctatgggac gagtaaatac tataacaaaa gaagaagcac aagatgcgga
     5221 ctttatactt atagcatctg accaaactat atctggaatg gaaagatttg atggtaaaaa
     5281 agtgataaaa gtcaaaatag gtatagcgtt aaaaaaacca gaagctgtct taacaaagtg
     5341 tatagaagct atttcttaat aaaaaaatct cactctaata tagaatgaga aaaatcaaaa
     5401 attaaaaaat tgtctaatgt aattataaca tattatcaaa aaatataaca aacttatata
     5461 ggaggattaa aaatgagtaa aaaaaataaa tctcttttaa gtgaacttaa gggacactta
     5521 atgacaggta tctcatatat gttgccactt gtaataggct catctttagt tgtagctatc
     5581 ccaaagctta cagctttagc agtgggtata acaagtttag atgcttataa aaatgtatct
     5641 ggtttttatc atattcttta cttaatggaa caagttggtt ggactggcat aggtctatta
     5701 aatacggtat tagcaggatt tatagcattt tcaataggtg agaaaccagc tatgggtgca
     5761 ggttttattg gtgggctcat agcaagtaat actaatgcag gattcttagg agcagtaata
     5821 gcaggtttct ttgctgggta tgtttgtaag tttttaaaga aaaaaattaa aatatctggg
     5881 tcagcatcag gaatgatgcc acttataata atgccactta taacagttgg attaactggt
     5941 tttttaatga gtgttgtatt agcaggacct ttaggaaaca taaatacttc attaaatgct
     6001 tgggtagctg aaatgtgtca aaatggaaca aattcggtgg tattggcttt aattttaggc
     6061 gcaatgatag gattcgattt aggtggacca gtaaataaag cagcttggat ggcaggaaat
     6121 gcattattgt tagagggaat ctatctacca gcgatattgg taaactgcgc aatagttata
     6181 cctccactag gatatggttt agctacattc ataagaaaat caaaattttc caatgcatta
     6241 gctgaaacag gaaaaggcaa tatagttatg ggtataattg gaataacaga aggtgcaatt
     6301 cctttcactt taacaaatcc attaaaatta gttcctgtta atatgatagg atgcgctttg
     6361 ggtgtaggat tatcagcttt gttaggtgta catgctataa tgcctccagt tggtggatta
     6421 tatggattta tatcaatagg ttctggatgg gcttacttag taggtgcaat atttggagct
     6481 ttagtaatag caatattatc aacaacttta gtagacttca atgaagatga aactacggaa
     6541 aatacagaat ttgatgaaat agagttagat ataatgtaaa ttaaaaactt aattttagat
     6601 aattgagaat tgagtgtact ctcaattctc aattaaattt tgggaggatt tttatgaaaa
     6661 agacaaaagt acatgtagtt ccacacagtc attgggatag agaatggtat tttacaacat
     6721 caaggtcaaa aatatattta atgaaagatt ttcaagatat attagacata ttggaagaaa
     6781 aagaagactt taaatacttt actatggatg cacaagcttc tctcttagat gattatttaa
     6841 aatggagacc tcaggataaa tatagaataa gagatttagt tcaaagaaga agattagtta
     6901 ttggaccttg gtatactcaa acagaccaat tggttatatc aggagaatct ataattagaa
     6961 acctttacta tggaatagat agatgcaatg aatttggtga acctatgagg gttggatatg
     7021 taccagattc ttttgggcaa tcagctcaaa tgccacaaat atacagaggt tttggaatag
     7081 atagttcatt attttggcgt ggagtttctg atgatatggt agatacaaca gagtttatat
     7141 ggaaaggttc tgatggttca aaagtattag cagttcaaat accatttggt tattactatg
     7201 gaggtaatat accagaagat gattcagatt taaaagaata tttaaaagat ataatcgtaa
     7261 aacttaaggt taaagcttct actaataacg tatattttcc aaatggattt gaccaagcac
     7321 caataagaaa aaaccttcct gatatattaa aaaaagctaa tgaaatagat aatgaaaatg
     7381 aatacgaaat aagtgatata gaaaactata tagattcagt aagaaatgaa agaaatgaat
     7441 ttacagagct taaaggtgaa tttttaaatg ctaaacatat gagaatacac aaatccatat
     7501 tctcctcaag gtcagacctt aaaataatga ataacaaatt agaaaattat attacaaatg
     7561 tacttgaacc gttattaaca ttaagttatt cacttggaaa tgaataccct cacctagtta
     7621 taaaagatat atggaaactt atgtttgaaa atgcagctca tgacagcata ggttcttgta
     7681 atagtgacac aacaaatgag gatgtataca caagatacaa acaagccaga gatttgagta
     7741 taaatttatt agatttgcat atgagactta taagtactca aatcaaaaat aataatgaag
     7801 aaataacatt aacagtattc aatggattcc cagaagtcag aaatgaagtt gtagaatttg
     7861 atacttatat tccaggtcaa gattttgtga taaagaataa aaatggaaaa attcttaatt
     7921 atataataaa agaaaaagaa gatattacaa attatatatt aactcaaaca ataagattaa
     7981 acccaagtaa aaaaatatat atacccaaaa ctgtatatag agcaaaaatt gcactttcag
     8041 taaaagatat accaccaatg ggatatactc aactagtttt tgaattggat actaaaaata
     8101 aatgtgaaat agaaacaagt aattgcaagg aaatagaaaa caaatactac aaaataacag
     8161 ccaataaaaa tggttcatta gaaatattag ataaagaatc taataagact tataaaaacc
     8221 aagctgtaat tgaagacaat ggagatgatg gagattcata caactattct cctccaagaa
     8281 aagatattta tgtttcatct ttagattctg tatcttcagt agaagtgcta aaatcaaaca
     8341 ttcaagaaga aatgatactt aaatattcat tgaccatacc agcttcactg gaagaaaggg
     8401 ctataggtaa agtttctgtg aaaatgccag taacaatgaa aatcacctta gaacctaaag
     8461 aacaaataat taagtttaat gttaatataa aaaataacca agcactatct catagagtaa
     8521 gagttttatt taatacagaa atagcatcta agttttctat agcagatcaa caatttggaa
     8581 taatacaaag accaacagta ttagaaaaag accttgcatt gtggaaaaga gacaattact
     8641 catggcaaga aaaacctata actatcgaac caatgcaaag ttttgttact ttagaggatg
     8701 gacaaagagg aatagcatta ataactggtt gtgtaagaga atatcaaata gttggtgata
     8761 atttagatac aatagcatta acactattca ggtcatttgg atatatggga agagaaaatt
     8821 tactttatag accaggaaga gcttctggag aaaaaataat agcaacacca gatgcacaat
     8881 tacttaaaga gttaaacttt gactttggtc tttacatata taacaataaa tttgatgaag
     8941 caaatgtagc taatacagct aagagattct taacacctat acaaatatat gaatatgctg
     9001 attttttaaa tggtagactt atatttgcat tcaatgacga aaaacaaata tatgaaaatg
     9061 aatttagttt gatgaatgta aataattcaa atttcacagt aagtgcaata aagaaagaag
     9121 aaaaaggaga tggaattgta gttagaatct tcaatggtat gaaaaatgag gatgctaagg
     9181 gaagtcttaa catcaataaa aatgtcaatg atgcttatag tgcaatgctc gacgaaatct
     9241 atgataatac tagcaagctt aaagtaaata ctaaaactgt agaggtaaat tctttatcac
     9301 attgtaaagt acaaacaata gtaattaaat aatataaaat agcttataca agatttcaaa
     9361 ataaattgct agtaccatgt taatagtaaa gccagatatt atgctattta taagctctgt
     9421 aacactaaat aacattttgt aaaataaaaa ggtgaacctt gtatattagg attcaccttt
     9481 tttaaataaa tcaacattct gttttaaaat aacatttata agctgttttg aaataatgct
     9541 tacaaattat tttaaaacaa tacctataaa tttttattta aagaatacac ataaatattt
     9601 ccagactctt ttatcctata gactttataa ttattaaaga agtctaatct aatttttgtt
     9661 aaaagtaatt gtaactttaa ataaatcacc atccacactt atattcatct taccaccctg
     9721 taattctata aaactttttg ctatcgctaa accaagacca gaaccttctg tgttacgtga
     9781 tttatctcct cttacaaatc tctccattat atcatctaca ctaaaatcaa tctcagatgc
     9841 agacatattt ttaagcataa tatttacttt attatctgtt tctaaaatat caatatacac
     9901 tcttgaattt ggcattgcat atttgcttat atttattata agattttcaa atactctaaa
     9961 tgtacgctga ctatctaatg gtaatattat tttttcatta gaaaagttat tctttactat
    10021 tagagatgaa tctgcaagtt tgtcatctaa ttctaaaagt gtctgcttca ttagagatac
    10081 aatatccaca tttactatat tcaaataaac atctccacta tttgccttgc ttacttcaaa
    10141 caaatcttct attagatgtt gcaatctttg tgactttctg tctaatgtat caatatacaa
    10201 cttacgattt tcatctgtta tactttcatc ttttaataaa tctatataag tgattataga
    10261 tgttagaggt gtttttaagt catgtgatac atttgatata agctctgttt tcatcttttg
    10321 actcttaacc tcttcgtcaa cagctttttt aaatccagtt tgaatatttc ctaattcgtc
    10381 ttttatagga ttaaaaaatc ctaaatcttt ttccatattt acatctaagt ttccatctga
    10441 aattttcttt gtaacatcta aaagttgatt atattcatat attgtctttt tagagtaatc
    10501 tgacagtatt ttaaataata ctactgaata tattaatgcg agtattattc caaagaacca
    10561 catactacac attataacta taatcaataa atttattccc aaaattgtaa ctatagtttt
    10621 atttggcttt tcttttagat ttattgttct aatttgatta ataactgttt ttcctgcttt
    10681 ttttagccat acaaaaaaca ttcctgtcac agtatgtttt gtaaaatact ctcttattcc
    10741 aacatttaaa atatgtttta aaagtattat aaatacaaat attattgaga atattacaaa
    10801 ccaataagtt aaattcatta aacttaccaa tatactagcc attttagcac ttatgtcatt
    10861 gtttataagg tactctacat aattcccact caaggtatta cttattataa cagtaggata
    10921 taaatctaca agcccctgta taatagcaag agcaataaag agtatctcta acggtattct
    10981 tgataatgta tcaaatccta gcaactcttt agattttcta tatgggaatg ctaaagctac
    11041 aataacaact atagctaata taatttttaa aatcattcca gaaatcttat aataagaatt
    11101 attttctaca ttgcttatat atgaaattat accatcatca aaataagtat ttccataacc
    11161 atactctaca ctatcacctt tacttattac tttaggtaca gcatatacta atgttatatt
    11221 ttttattgga gataacttat atgaaattga atttcctcct aaacttgtat ccattaaatc
    11281 ttctcttgct ttagataaca acatttgatt aataattttt tgatttactc cataagaatc
    11341 cacaatattt acttttccat ctttatcaaa attcattaca acgtagaatc tatagttttc
    11401 ttttatatct gtaatctgct tttgtctttc ctcatcattc atttctgtat tttttgactg
    11461 attttcatca gaattttctt ttgaagcatc attttttgtt gtatcaagta ctcctaattc
    11521 tttattatca ttaaaaaatt caaaattgct ttctttatca actaaatagt actttaaatt
    11581 ctttcctgac gctttaatga aatgctccca attttcaaag ttttcattaa aatctgcatt
    11641 aatactatca tctgtatagt aagtttctga accatcataa gtatcttttt ctttacttac
    11701 attttttata tagttcatag tttcttcatt gataaatagt tttgatggtt gtatcatctt
    11761 accttcttgt tcttccttca tattatgata taaaacataa cttcctttat atatactatc
    11821 tacaaagctt tcctgttcaa atggattctc tgctccacct ttagcacttg cttgtatagt
    11881 agaatattga ctaatcatac ctaatgatgc tgataacata agtataaaaa ctataatagt
    11941 aactatatat ttatctttaa attttttcaa ttttgtatcc aacaccccac accaccttta
    12001 aatatttagg attttttggg tcaatttcta tcttttccct tatattcctc acatgtacca
    12061 taactgtatc tgtatttaca gcattctcat tccaaactct ttcatatatt tcatcagcag
    12121 aaaaaactct tcctgcattt ctcataagta aacttagtat tttaaattct ataggagttg
    12181 tttttatata ctttccatca atactaactt cttttgtatt ttcatttaat tctaatccgc
    12241 ctattgcaaa cacactatcg tcactttcta ccttttgctc tttatttcca accatgttta
    12301 gatacttagt gtaccttcta agttgtgaat taactctagc caatagttct aatggcttaa
    12361 aaggttttgt tacataatcg tctgctccta tattaagccc cattattttg tcaacttcct
    12421 ctgattttgc agatagcatt attactggaa aattatagtt ttctctaagc ttcattgtaa
    12481 gtgttatacc atccatttta ggcatcatta tatcaactat agctaaatgt atttcttggt
    12541 tttctataac cttaagacct tctaatccat tgtatccctt aaaaacatta tatccttgat
    12601 ttaatagata tatttctata gcatctgcta tctccttttc atcttctacc accaaaatat
    12661 tatatgaatt cattctaatc tcctttatat taatttataa attttcttta catatatgat
    12721 tatacaatat gatagaagtt tttccattta tatatatatt tatatctttt gttctggtta
    12781 tttgtattta atgt
//
LOCUS       DAMJZE010000114        12674 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJZE010000114 DAMJZE010000000
VERSION     DAMJZE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.115"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            153..1214
                     /locus_tag="PC541_002636"
     CDS             153..1214
                     /locus_tag="PC541_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2840087.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1305..2918
                     /locus_tag="PC541_002637"
     CDS             1305..2918
                     /locus_tag="PC541_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2840088.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3193..5178
                     /locus_tag="PC541_002638"
     CDS             3193..5178
                     /locus_tag="PC541_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2840089.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            5183..5617
                     /locus_tag="PC541_002639"
     CDS             5183..5617
                     /locus_tag="PC541_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2840090.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5646..6206
                     /gene="srlA"
                     /locus_tag="PC541_002640"
     CDS             5646..6206
                     /gene="srlA"
                     /locus_tag="PC541_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2840091.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6270..6638
                     /locus_tag="PC541_002641"
     CDS             6270..6638
                     /locus_tag="PC541_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2840092.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6663..7229
                     /locus_tag="PC541_002642"
     CDS             6663..7229
                     /locus_tag="PC541_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2840093.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7270..7635
                     /locus_tag="PC541_002643"
     CDS             7270..7635
                     /locus_tag="PC541_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2840094.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7736..8509
                     /gene="srlD"
                     /locus_tag="PC541_002644"
     CDS             7736..8509
                     /gene="srlD"
                     /locus_tag="PC541_002644"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2840095.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            8858..11002
                     /locus_tag="PC541_002645"
     CDS             8858..11002
                     /locus_tag="PC541_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2840096.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            11090..11425
                     /gene="spoIIAA"
                     /locus_tag="PC541_002646"
     CDS             11090..11425
                     /gene="spoIIAA"
                     /locus_tag="PC541_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2840097.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            11439..11867
                     /locus_tag="PC541_002647"
     CDS             11439..11867
                     /locus_tag="PC541_002647"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2840098.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            11890..12654
                     /gene="sigF"
                     /locus_tag="PC541_002648"
     CDS             11890..12654
                     /gene="sigF"
                     /locus_tag="PC541_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2840099.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         5100 a         1265 c         2378 g         3931 t
ORIGIN      
        1 aaaataaaaa attgtcgctt taaagtataa aacagattat atgttaataa tgtggtgaaa
       61 ttatacccat atataaataa aataaaacaa ataaatagct aattaaggag aaaatatttt
      121 ttattttttg tttaggataa ataaatttta ttatggaaaa aagaaaaagc atacatcttc
      181 acaaactgaa tatttattta atagggggag gtgatagatt gtttatcaca gtggtattat
      241 ttttagtagg tttcctatta attactaaag gcgcagatat ctttataaat tgtactgtag
      301 agataggtaa aaaaacgaat atatcagaaa ttatacttgg agctactata gttagttttg
      361 caacaacatt acctgagttt acagtgtctc tacttgcttc tattgatggt catactacta
      421 tgagtttagg aaatgcagtt ggttctataa tatgtaatac agggttagca ttagggttgg
      481 ttgtttttat aagtccattt aatgtagata aaaaaatgtt tttttctaag tctttactat
      541 taatagtatc cgttatagtt ttaattttat tgagtttaga tggagttata acaagaggtg
      601 attcattatt acttataata atattaatct tttatatggt taataattat agaagtgtcg
      661 ttggaaaatc tgatactaaa aatagaaata ttaaaaataa cactattaaa gacatatcta
      721 ctaaaaaagg gaaaaattat agaggttttt ccgtattaga aattgtaaaa atactattac
      781 tttttgctac aggattgata atgatgataa taggttcaca aatccttata gagagtggag
      841 taataatagc tagtttttta aatatacctc aaggtatagt aagtttaact ataatagcac
      901 ttggaacttc tctgccagag atagtttcgt cgattactgc tataagaaaa aatcatcatg
      961 aaatatcagt aggaaatatt ttgggtgcaa acatattgaa tatagtatca gtaatagctg
     1021 tatcagcaat accaaataac ataccaatac tatctcaaaa tagacaattg gatataccat
     1081 tcatgatatt attattatta atagtaataa taccaacact taaatctaat aaacttagta
     1141 gaatacaagg tatattgatg ttattcacat attttctgta catatcaatt ttgtatttca
     1201 tgtatattat ttaactatac tgtttaaaat attaattgca gaatatccta aaaaagtata
     1261 taatggatag gttaagatta attgataacg gagaggaata atatatgaat ataacaaagt
     1321 ttgaagattt accaataagt gagggaataa aaaaagctat agctgaaatg gggtttgaag
     1381 aaccatctcc tatacaagca cagtctatac ctgcgatttt gtcaggtaaa gacgttatag
     1441 gacaagctca aactggtacg ggaaaaacag ctgcttttag tatccctata ctagaaacaa
     1501 tagacccaaa taatagaagt ttacaagctg tagtgctttg tccaacaaga gaactggcaa
     1561 tacaagtatc aacagaaata agaaagttag ctaagtattc gcacggaata aagacattac
     1621 ctatatatgg tggtcagcca atagatagac aaataaaatc tctaaagagt ggtgtgcaag
     1681 ttgtaatagg tacaccagga cgtacaatag accatatcaa tcgtaaaaca ttaaagatgg
     1741 ataatgtaaa aatgattata ttagatgaag cagatgaaat gctagatatg ggatttagag
     1801 aagatataga aatgatttta agtaagattc cagaagaaag acaaacaact ttcttttcag
     1861 ctactatgcc aagaggaatt ttggaattaa ctaagagata tcaaaaagac ccagagcata
     1921 taaaagttgt tagaaaagaa cttacagttt caaatacaaa gcaatactat atagaaacta
     1981 ggtcatcaaa taaattagaa gtattatgta gattagtaga tgtatatgac cctaaactat
     2041 ctgttgtttt ctgtaatact aagagaaaag cagacgaatt ggtaggagat ttacaagcaa
     2101 gaggatattt tgcagatgca ttacatggtg acttaaaaca gactcaaaga gatatagtaa
     2161 tggataagtt tagaaatggt acaatagata tacttgttgc tactgatgtt gcagctagag
     2221 gtatagatgt tgatgatgtt gaatgtgtat tcaactatga cttaccacaa gatgaagaat
     2281 attacgttca tagaattggt agaactggtc gtgctggtag agaaggtatg tcatttactt
     2341 ttgtatttgg aaaagaaatg agaaagatga aagatataga aagatatact aaatctaaac
     2401 taataaagca taatatacca acaataactg atgttgaaga gaaaaaagta ggtactttct
     2461 ttgcacaagt taaacaaact atagaagaag gtcacttaac taaacaatta caatggttag
     2521 aaggtttctg taatgatgaa gattatgcaa tggttgatat agctgctgca cttgtaaaac
     2581 tatctctagg tgaagaaatg aaagaagaaa taatagaaga aaaacctaga agagagcgtg
     2641 gagatagaaa aggtggtact ggagctaaag atggtatgat tagattattt atcaatattg
     2701 gtagaaatca aagagtacaa gcaaaagata tagttggtgc catagctgga gaagttggaa
     2761 taccaggtaa agtagtaggt actatagata tatatgataa gtatactttt gttgaaatac
     2821 ctaaaaaaga tgcaaaaaca gtaattgaaa agatgaaaga tattaagata aaaggtaata
     2881 agattaatat agaaaaagca aataagaaga aaaaataacg tatagtatac aaaactataa
     2941 ttagaaaata tataaaataa tatattggat atttaaaaag cctgatgtgt tttcaaaaca
     3001 gatagtagat aatctgtaag agttaacagc ataaggcttt tcttgtttta tatgatgtta
     3061 taattttcca gattgttgtg aaaaatgtat attagttcct ttttttcaaa tctgatttga
     3121 aaaagagaat attaattatc tatatagttt agattataat aaaaaatgta agataacaaa
     3181 ggggagaaaa agatgtcaat gacttatatg gaccctaggg tttttttgat tttagaaatg
     3241 gtgttagaag aagacacaaa tactctagaa caattttcaa atgttttggg tgttagtact
     3301 aggactatca gaaactatat aaaccagatt aatcgagaat taaaaggtgg tattgctaaa
     3361 atagttaaaa atgaaaaagg tacatatagt ataagaattg aggatgaaag taaattaatt
     3421 gagattgtaa attttaatag aggaaagcta tcaaatttta taaatttaaa ttcacctgat
     3481 gaaagaataa attatgttct tgatatttta actatgacca acaatccaat taccatagat
     3541 gatttggctg aagagatatg tataggaaga acaacattaa taaaggattt aaaaaaagca
     3601 gacagaattt tagctgaata tgatttagaa ttaaaaagga agccaaatat aggtatgatt
     3661 ttagttggtg aagaaatgga tataagatta ttaatccttg atagattgta tgaaaattat
     3721 attgatgtct tggaaaacgt taatcagata aatttagtta aagattatga tattgaatgt
     3781 cttagagatg aattaaaaaa gttatttaaa aaagaagaac tctacataac agaacaggtc
     3841 ttgagagatg ttgaaagata tataatcatg tctgtcttaa gaaatctaaa tgggtacaaa
     3901 tttgaaaaga tagataagag atttgaagtg ataagatgtt cagatgaata tactttagga
     3961 ttaaagctca aagctttatt ggaaaagata tttaatatag ttttaaatga aaaagaaacc
     4021 atatttttga caatgccatt aataggtaga aaagctccta gtacaaaatt agcacttagc
     4081 agtataaaga taaatgatag cgtaaaggaa gttgtagatg aagtaactag ttttttatta
     4141 gagacctcag ggattgattt taaagaagat aaaaagctta tagaaaattt ggaatatcac
     4201 ttgtattttg cacttaatag aatgagattc aacataaggg taaaaaatcc tcttttacaa
     4261 gaaataaagg aaagatatac atttccatat agtgttgcaa agatagctgc aatgataatt
     4321 gaggaaaagt ttgacttgaa aatatgtgac cacgaaatag gatatatagc attacatttt
     4381 ggaagttatt ttgaaaaaaa ttcaagaaaa gttctaaatg taaatagggt agctgttgtt
     4441 tgtggaacag gtcttgggac tgcacaactt attagaataa agataggaaa aatacttgga
     4501 gaaagtgtag aagtaaatac tttttctgat atagaagcta aagctaattt agatttatta
     4561 aagtattatg atttagtatt tacaacagta gatttcaaaa cagacttaga tgttccaatt
     4621 tttagagtaa atgcgttatt taatgaagaa agtataaaga aagagataga aaatgcaata
     4681 cttttgaaaa atagtataaa tcttaagtct ataaaaaaga ttgaaaaaaa tatccctttt
     4741 ataggtaaat taattgaaga ggataagttc tttatattga ataagagtac gtttatggaa
     4801 aatttagaag atatgttaga aaaattagtt cataaaggaa ttattgatat agagttcaaa
     4861 aaaaaggtat taaaaagaga aaagaaatct tcaacagcat tagataatta tatagcattg
     4921 cctcactcag ttaatactaa tggggaaaat ctttatctgg catttggaat attagaaaaa
     4981 ccagtgattt gggatacaaa ggaaattaga ataataatat tgatgataat tccagataag
     5041 aatgtagata gtacagacct gataataaaa agttatgaag aagtacttga agtagggaga
     5101 gataaaaaga tggtagaaga acttgctaag gcaaaatcat ttgatgaatt ttataaaata
     5161 attaccaaga ggaggtaata atatggaccc agtaattttt attatagcat cacttattac
     5221 agtgtattta ttaaatattt ttcttggata cttacagctt aaagatttta ataaaaatta
     5281 tattgaacta aaaagaaaag gaagagtggc aattggcaga aaaaaaggaa gaatttcatc
     5341 gggtacaatt gtgttgatac ttataaatga tgatggtgtg attgtcgaga ctagaaaaat
     5401 gcaaggtgtt acagtattgg caagagtaaa gtcatttgaa ggtttggtag gaaagaactt
     5461 agagtatata gaaaaatcag atttaactgt ttacaataaa ctacttaaaa aagcaatact
     5521 agatgcagtc aaacagtaca taacatttga gaaaaataaa tgtgaagata caaataatct
     5581 gagtactgat gaagagtgta ggatggaagt agtttaaaaa aatatattta taaggaggag
     5641 ttaatatgac gcaatttttt caagtattag caagtggtgc agatagtttt atgaatttat
     5701 ttagggcagg tgggcaacaa tttgtaggtt ttgtaacaga tatagtacca cttttagtag
     5761 cattattagt tacaatgaat gcaattatca actttattgg tacagataga gtcgagagac
     5821 ttgcaaaaaa atgttctgca aatatattta ctagatattt gatattacca gtccttggta
     5881 catttgtatt tgcaaaccca atgacactat cattaggaag attcttgcca gaaaagttta
     5941 aacctagtta ctttgcagca gcaagctttt catgccatac aatgaatgga ttattcccac
     6001 atatcaatcc aggtgaacta tttatatatc taggaattgc aaatggtata acaactttgg
     6061 gattctcaac agcagattta gcagttagat atttgttagt gggtatagtt gcaaacttta
     6121 ttaaaggtgt agttactgac tatacaacaa aatttgtaga gaaacaacaa ggaatagcat
     6181 taaattcaga tataaagatt ggataattac tttaaggtat aaataaattt ttcaaataaa
     6241 aagatagtgt atagaggagg ataaattata tggcatttaa caaagttaaa atagtaaaag
     6301 gttctggagg atggggtgga ccattgataa tagaaccaac agaaaagaag aataaggtag
     6361 tatatgtgac tgggggagca aagccagaaa ctgcagtaaa aatagctgaa cttacaggat
     6421 gtgagttaat agatggattt tcacagggtg ttaaagatga tgaaatcgca tgtgtaataa
     6481 taaattgtgg tggaacattg agatgtggta tatatcctca aaagaaaata ccgactataa
     6541 atataatgaa aacaggaaaa agtggaccat tagcaatgtt tataaaagaa gatatatatg
     6601 tttcaggggt aaaaccaaca aatgtagagc agttataatt gaggtagtag gagggaagaa
     6661 atatggctta tgatactaaa aagaagataa gtgagcagag tcaaggaatt gttgcaaaaa
     6721 taggtatagc actaggaaat gtgacaagta ctctttttca agctggtagg gatactatag
     6781 atacattaat acatactata ctgcctttta tggctttcgt tgcagtccta ataggtataa
     6841 taaatcaatc aggtatagga aattgggtag ccaatttact tactccatta atcggaaatg
     6901 ttggaggttt agttgtctta tcaataatat gttcatttcc gttgttgtct ccgtttcttg
     6961 gaccaggagc tgttatagct cagatagtgg gagtattggt tggtacagaa ataggaaaag
     7021 gaaatattcc accaagttta gcattaccag cattgtttgc aataaactca caagcagctt
     7081 gtgactttat accagtaggt cttggtcttg cagaagctga acctgataca gttcaagtag
     7141 gagttccgtc agttttatat tctagatttt taacaggtgc accaactgtt ctattagcat
     7201 ggttagcaag ctttgggtta tataaataga agaatacttt ataaataatt agagagaggt
     7261 tcagttgata tggaattaat atacagtact caaataaata aaataggaga taatgcaaat
     7321 gaatttttac aacataatat gtttgtaact ttcaaagata atgctcctca agagttagaa
     7381 aattactgtt atatacattc tgaaaataat ttagtaaaag aaatacttac ggatgatgtg
     7441 ctatcaataa ataatgttga ttacaaaatt acttctgttg gtgagttagt aaaccaaaat
     7501 ctttcagaat taggtcatat aacatttaag tttacaggtg aaaaagatgg agcaattgga
     7561 ggaaccttat atcttgaaga aaaagaaata gcacctattg gcgtaggtac tatactaaaa
     7621 ataattagaa aataaaattt aaaacgaatc taatcatatt ataagtaaat tatagataaa
     7681 agtcaattta actaatcaag ttaactttta atgtgataaa aaaggggaaa tgaaaatgag
     7741 aaaagtggct ttagtcgttg gaggaggaag aagtttaggt gaatatttat ctaaacattt
     7801 agctaaagta ggttatgatg ttgctattgc agatttaaat gaagacaacg caaaaaaagt
     7861 agctgaagaa ataaaaactg aatttggttg taaatctgta ggatatagtt gtaatgccat
     7921 agatgaaaga caggttataa gtactgtcaa agctattgaa aatgattttg atagaataga
     7981 tttattagtg tacaatgcag gagtggctgt aagtaaaaaa ataactgatt ttggatacaa
     8041 agattttaaa tttattgtag acgtaaattt aaatgggtac tttctatttg caagagaagt
     8101 atctaaagtc atgatagcaa aaggtattga gggtagaata atacagataa attcaaaatc
     8161 aggtagagtg ggaagcaagc ataattcagg atattcagca ggtaaatttg ctggagtagg
     8221 acttactcaa agtttagcgc tagatttggc agaacataag ataactgtaa atgcactgat
     8281 gctcggtaat ttacttgatt ctgatatgtt tgaaagttta attcctcaat atgcagtaaa
     8341 attgggaata cctgaatcag aagtcaagca agtttatata gataaagtac ctttaaaaag
     8401 agggtgtaga tttatagatg ttgcaaatat gcttacattt tatgcttcaa aggaagctga
     8461 atactgcact ggtcaatcta taaatattac tggtggacaa gttatgtaag gtttttatgg
     8521 agtgtctcaa gatgaactct ttagttcatg aggcactttt ttgtataaaa ttaaatatat
     8581 tttcagcatt taaacaatga aaaagaggtt tatctttatt ttgagatagc ctcatttttg
     8641 taattaaaaa acatagtgtt taagtaaaca tataagattg ttagtttttt aaaagagaat
     8701 tttttaataa tattatattt atgaagtatt tttaaactta ttttgataaa atatagctat
     8761 gttttatata ctgtagtttt gaacatagaa agtttaataa attatagtat atatgttaat
     8821 ttttaataag gtaagaaagt agaaagaagg atattttatg gatataataa aaaaattaag
     8881 tcatgggtgt acattagatt gtcatgactg ttgtaaattc aatgtttata ctaaaggaaa
     8941 taatgtagta aaaattgaag gagataaaaa tcatccttat acaaaaggtt ttatatgcaa
     9001 aaaagggatg gcccatttag atagattaaa tcataaagat agaataaaga ctcctatgct
     9061 taaagttgat ggagtatgga aagagatttc ttttgataaa gctatagaaa taatggcaga
     9121 aaagcttaca tactataaag aaaagtatac atcaaagtct gtcatgcatt atgaccaata
     9181 tggaagtggg tctgtgctaa aatacatagg agatatattt tttaattttt atggaggtgt
     9241 tagcagacat aaaggtggtc cctgttggag tgcagggatg catgctcaaa aatatgattt
     9301 tggagtagct aagagtcatg ctatagaaga tatgttaaat agtaaaagta tatttgtgtg
     9361 gggaaaaaat ccagcatata ctacaattca tactatgcaa ataattaaaa aagctaaaga
     9421 aaaagggata aagatagtag tgattgaccc catatataca aaaacagctc aaatagcaga
     9481 taagtatgta caagtaaatc ctggaacaga tggagcttta gctatagcta tggcaaaaat
     9541 tatagtagaa gataaattat atgatgaaga atatataaac tcgtatgtta ttggttttga
     9601 agaatataaa aaatatttat cttcattgga attaagtttt ttaattgatg aatgtggtgt
     9661 taaagagaat gatataagag agttagtgga tttatatact aacaaatatt caagtataaa
     9721 tgttggatat gggcttcaaa aatataaaaa tggtggaaac acaataagag caatagatgc
     9781 tttaggtgct ataactggtc aaataggatt tagtggtgga ggggtaaact atgctaataa
     9841 agtttatccg agtgttataa attctgaccc ttacaatagc caatcatatg gtgaagatag
     9901 ggagttttat gtaagtaata tcagtaaatt tatagaagaa tctttaaaaa atacatcaaa
     9961 taaagtaaat tatgcatcag atgaacttga tatgacatca aataaagtaa attatgtatc
    10021 agatgaactt gatataacat caaataaaac agattatata tcaaatgaat tgtataattt
    10081 atctaataag tcaatcaaag gtaatattcc tataaagatg gctgtaataa ctaaaagtaa
    10141 tatgctgaat caactgccag atttagtaga attggagagg gtgttttcaa aaattgaatt
    10201 taaagtatgt tttgatatgt ttataactga tacagctact ttatgtgaca tgtttatacc
    10261 gtgtacaaat acactagaga gtgaagatat aatttatagc tcaatgacca atccatatat
    10321 aacctataat gaaagagctg taaagccagc acataaattg atggatgaat attacttctt
    10381 tatggagcta gctaaaaaga tgggattaaa tgattatcct tttgtagaaa agagaactta
    10441 tttagaaaaa gtgattgaac cacttaagcg ttttgacaaa aatctggata tagaaaaact
    10501 taaaaacaat tactttacta tacataaccc tgttgcttgg gaagataaga agtttgagac
    10561 tccatctgga aaatacgagc tttattcaga aagtataaaa aacttgggaa taagtccaac
    10621 acctgtatat ataagcaata aatataaaga aatagaagat aaaaatattt cttttagatt
    10681 attaactaat catcatgcag atactttatt tagtcagcat tttatggaca aaaaatctat
    10741 agctcaagca tatataaatc ggagaatggc taaaaaagta ggtattgagg acaaagatat
    10801 tgtgatattg aggtcaaaaa aagcaaaaat aaatgtacaa ataaatatag atgatggagt
    10861 aggtaattat atagtaaaaa tgtatgttgg ttggtggaag aaacatggaa atcctaatag
    10921 tttaacagac actggtatat cagattttgg gggtcaggta acatataatg aaagtatggt
    10981 agaaataata cgacaaaact agttaaattt cattgaagga ataaaaatat aattatagaa
    11041 ttgattaaaa gttactattt ttacaaaact attgaaggag ggatttttga tggtaaatta
    11101 ttccttagaa cataagaatc tatacataga atttatgtgc tcagaactag accaccatgt
    11161 agccaatgaa ataagggaag aaatagataa cttattaagt gtaaatcaag taaaaaatgt
    11221 tgtatttaat tttgaaaata ttaattttat ggattcttca ggtataggcg tcatcatagg
    11281 tagatataaa aagatttcta atgaaggggg acgagtttca gtaattaata taagttcacg
    11341 agttaagaag atttttgatt tatctgggtt aaataaaatt attggcattt atgatacgta
    11401 tgaagaagct ctaagttctt tgtagggagg agtatgtgat gaataatatt atggaagtta
    11461 aattttcggc aatatctgaa aatgaaagtt tagcaagagt tatagtagca tcctttgcag
    11521 ctaagttaga cccaacagta gatgagttag tagatataaa aactgctgtc tctgaggctg
    11581 ttacgaatgc tataatacat ggatatgaag aagacagttc taagtttgtt ttcttaagat
    11641 gtgagataga aggaaatact ataaagatta tagttgaaga tgaagggtat ggaatagaaa
    11701 atgtggagaa ggcaatggaa ccactttata catcaaaacc agaacttgat agatcaggaa
    11761 tgggattcac agtaatgaaa agctttatgg acgatgtaga ggtaagttct gtaaaagata
    11821 atgggactag aattgaaatg actaaaaaaa taaatgtgcc taaataaagt ccaaaagtga
    11881 ggttttatta tggaagtaac tgttgccaga gaagaaaaaa aacctcttct tagtcatgaa
    11941 gagactctag aacttattga aaaagttcaa aatggagatg aagaggcaaa agaaatatta
    12001 atatcaagca atttaggtct agttaggagc gttgtatcta gatttttaaa tataggatat
    12061 gatagagaag atttatttca gttaggttct ataggcttaa taaaatcaat atataaattt
    12121 gacccaaaat ttaatgtaaa attctctact tatgcagtac caatgatttt aggtgaaata
    12181 aagagatact taagagatga tggtatgata aaagtatcaa ggtcgttaaa gcaaatagct
    12241 ataaaggcaa agatggaaag tgaagcttta actaaaaaat taggtagaga gccgagtata
    12301 gaagaattag ctaaagcaat agatgtagaa aaagaagatt tagtaatggc tatggaagct
    12361 aatttcaatg ttgagtattt gcaaggagtt attcatgaag aggaaggttc tccaatatgc
    12421 cttattgata aaataagcat gaaaggagaa agtgaagaag aaaaagtagt ggataacata
    12481 ttgttaaaag acatacttgg aagactggat aagagagagc gtcaaataat agttctcaga
    12541 tactttgaag acatgactca aagtgaaata ggagagatgc tcaatatatc acaagttcaa
    12601 gtatctagga tagagaagaa agtattgtct aagttaaaag aatatatatc gtgataatac
    12661 ttatgatatg taga
//
LOCUS       DAMJZE010000115        12493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJZE010000115 DAMJZE010000000
VERSION     DAMJZE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.116"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            333..1268
                     /locus_tag="PC541_002649"
     CDS             333..1268
                     /locus_tag="PC541_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2840100.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1360..3231)
                     /locus_tag="PC541_002650"
     CDS             complement(1360..3231)
                     /locus_tag="PC541_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2840101.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3421..4347
                     /locus_tag="PC541_002651"
     CDS             3421..4347
                     /locus_tag="PC541_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2840102.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4486..4632
                     /locus_tag="PC541_002652"
     CDS             4486..4632
                     /locus_tag="PC541_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840103.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4737..5261)
                     /locus_tag="PC541_002653"
     CDS             complement(4737..5261)
                     /locus_tag="PC541_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2840104.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5314..5538)
                     /locus_tag="PC541_002654"
     CDS             complement(5314..5538)
                     /locus_tag="PC541_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840105.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5735..6859
                     /locus_tag="PC541_002655"
     CDS             5735..6859
                     /locus_tag="PC541_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2840106.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7333..8157)
                     /locus_tag="PC541_002656"
     CDS             complement(7333..8157)
                     /locus_tag="PC541_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840107.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8505..9560)
                     /gene="ispG"
                     /locus_tag="PC541_002657"
                     /gene_synonym="gcpE"
     CDS             complement(8505..9560)
                     /gene="ispG"
                     /locus_tag="PC541_002657"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2840108.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9743..10747)
                     /gene="rseP"
                     /locus_tag="PC541_002658"
     CDS             complement(9743..10747)
                     /gene="rseP"
                     /locus_tag="PC541_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2840109.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10760..11914)
                     /locus_tag="PC541_002659"
     CDS             complement(10760..11914)
                     /locus_tag="PC541_002659"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2840110.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(12078..12281)
                     /locus_tag="PC541_002660"
     CDS             complement(12078..12281)
                     /locus_tag="PC541_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840111.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         4162 a         2101 c         1303 g         4927 t
ORIGIN      
        1 tcaaattaaa tataacttat atactttatg tgtaaattat ggtaaaaata ttatttaatc
       61 aaatcaaaac ttgatttata tacctggaaa aggtattata ttaaagagtg cttaaaatac
      121 gcatttcata aatagctata tataaaataa taaataagga gagtgatggg caatggatac
      181 tagtccgttg cggagagaat tttttacatt ttcatcactt aataatatct aaaatcaaaa
      241 ctaatacata tattcagtta tataaattag ttaattaata gatactatta aatgatgaga
      301 tttatataaa actgaataaa ggatgtgttg ccatgataag atattacaaa actatagatt
      361 caaaattaga gaaacttagt ttttttgaag atggctgttg gataaatctt gttgaaccta
      421 atcatagtga aatcaacgaa atttctaatt tattaaatat agatgtagaa agcatagaat
      481 ctgcactaga tgaagaagaa cgctcaagaa tagatgttga agacaatcat actcttatac
      541 ttatagatat accagtagac gaaagtgact ctaattcttc tcattatact acaataccct
      601 taggtattat actaactgaa gaagctattg taacagtttg tgatgcacaa actaagattt
      661 taaatgactt tatagttggt catattaaag atttttttac gtttaaaaag actcgctttt
      721 tgcttcaaat tcttcacaaa aatgcagctt attacttgca ttacttaaga aaaataaata
      781 aaatgacaat cataatagag cgagaaattt acaaatcaat gaaaaacaaa gaattggttc
      841 aattattgga attagaaaaa tctttggtat acttctctac ctcactaaaa tctaatgaac
      901 ttgttttaaa caaaatggtt agaactgctg gtataaagaa gtatccagat gatgaagacc
      961 tattagaaga tgttatagtt gaaaatagac aggctcttga tatggctaaa atatatggag
     1021 atatattgag tagaattatg gatgctttca gtgcaattat aagtaataat cagaataatg
     1081 ttatgcaaat tttaacagta gttacactaa ttttctcaat acctacaata atatcaggat
     1141 tctttggtat gaatgtcatc aacatgccat tttctaataa cccaaacgga ttttggataa
     1201 tacttcttat tacctctata atatgtatag tgattacatt tttcatgtca agaaataagc
     1261 tattataaaa tattaataaa aaggccatta gaattaattg atttacattt atcctaatgg
     1321 cttttatata tttaaatcta tataatctta taatactttt taactatcat gttcttcatc
     1381 tataatcatt tcagtttcaa gcccttctag cattgaatct aaactttctt ttatatttag
     1441 tatataaatt ggtacatact cttcttcatc tattacctct tcttgcaact taaatggagg
     1501 aattaatact tcatcaaagt ctatatttaa atttggaaag ccttctacta tctcttcaca
     1561 attatcgtac tcttcatttg ctaaagaaat gtacatatct aggctctttt tataataaag
     1621 acctgcaagc atatactcac acttcttact tccatattta ataccttctt tataatattt
     1681 ctcagcttct gcgtcatcat ttaattttaa ataaatttga cctaaattat atattgattc
     1741 aacacaacct tgtgctctag ccttttcata ccaaaattta gaatttacat aatctttata
     1801 gaacatatca taaattaatc ctaccatatg ttgagcatac acattatttt cattggcagg
     1861 tgtatcaaac atcattttag actcttccaa tttaccttct ctaaaataaa ttctacctag
     1921 atgtatacga gcattcatat ggtttttgct acttgccatt tcaaaatact ttctagcatt
     1981 ttttgtattt cctaagctct catatataca ccccaatcta taactagacc tcgtatctcc
     2041 attttcagat gctttcttat accaacttat tgcttcttca atattattta aatcttcata
     2101 gatatttcct aatttgattt gacaagatac attatttgga atcctcgaat aatacgaaat
     2161 cgctttttct atatcttgat tctgataata taaatcccct agattttcaa gggaactttt
     2221 gcatccaaca gctatagcat cctcataata tttaatagca ttttcataat ctttttcttc
     2281 aaaatatata cctgccagat tattttttgc ttctgttata tcatcttcag cagccatttt
     2341 ataataagtt tttgccttct ctatctcatt ttctctatta tataaattag caagtctata
     2401 cttagaaagt gtatgatttt tttctattgc tttctcataa taataaattg catctcccag
     2461 ctcttctttt ctatcatata catatgcaag cctatactca gacttcacat catctctatc
     2521 agcactcttt ttataatact tctctgcatc ttctaaatta tcatttattt ccataatcat
     2581 acccaagtta aagcaacctt tcatatgacc tgacttgtac aactgtttat ataaacttac
     2641 tgttgattta ttatctttct tttttccatt taatattgct aaattatatt ttgcatcatc
     2701 atctaaaagc acagaacctt ttttatagta ttcatatgct tcatcatctt tctccaaaaa
     2761 agtatataat acccctaaat taaaacaagc tttacctaaa ttcttgtctg ctgctgtttt
     2821 taaccatttt tcacacctta caaaatcttt cattttaaaa tggcatacac ctaaattatt
     2881 ttgtgcttgt aaataaccta aatttgctat cttttcatac caatatatag catattcata
     2941 gccatctaac ttatagtaca tattacctaa atcaaaagca gcatcttcac ttccactttt
     3001 atatgccatc gaataccact tttctgcttc tttaaattgt tttttacttt catatatatt
     3061 tcctaaagta tattgagatt ttacgtaatt atccaatgca gcactcttat acaactctat
     3121 tgcgctttcc atcttaccca tcttttcaag gagggcaccc ttagtatgta cgactttcgg
     3181 gtcattagat atatttttta ttttttttat agaaaatttt gtatttttca ttattcgcta
     3241 cctacccatt ttataaaata tattctctca aacattatta tacattataa tctacaatta
     3301 atgctataat attgaatttt taccttttgt cactatattg taacattaaa ttttaaattt
     3361 tataatcatt agattttatt atgtaattgg tatataatgt atatataggg gggacatttt
     3421 atgaatttca gcaatatttt tatccaagtt gcagttttat ttataattat tttagttgga
     3481 tattttgtca gaaaattcaa cttattagat gaccactgca catctaagct ttcaactttg
     3541 acaatgacag tttttttacc atcaatgata attagctcca tgcaaattaa ctttgatagt
     3601 aaaatgatac aaaaaatact attgttatta tttacatctt tgattatgta tatagtttca
     3661 attataattg catttttatt gaaatacatt ttaaaatgta atgataaaaa agatttggga
     3721 atataccagt atatagttgt tttttcaaat gttgctttta tgggttatcc tgtaattgaa
     3781 gcagttttag gtcatgaagc aatattctat actgcaatat tcaatttgcc ttttaactta
     3841 ttttctttca ctttaggaat ttatctttta tctaaaggca gtacaagtag aggtttttca
     3901 ataaaatcac ttataagccc tgctacaata gctattgtta ttggactttt tttatttgta
     3961 acaggactta ggttacccca atttataaac gagccacttg agatgttggg aagtataact
     4021 acaccaatat ccatgattat cattggaagc ttacttgcta attcttcagc tctcgattgt
     4081 tttgtcaaca aaaaattata tatagtcact tttataagat tattggtact tcctgtgatt
     4141 gtgtatttta ttctaaaagg atggataaat gataagatga ttttagctat accagtagta
     4201 atttcttcaa tgcctgctgc tgcaaatact gctataatgg ctaatcaata tgactccaat
     4261 ataacactag cttcacaatg tgtgtttttt acaacactat tttcagttat atctatacct
     4321 tttataagta ttttactctt atcttgattt atttttttat gtattatata ataacatatt
     4381 caataaatta caaatataaa cattttattg tagtatattg atggaatttt ttcctagata
     4441 tttgtcaata atatctatat gtattataga ataggaggct taaatatgga tatggattta
     4501 ctctttaaaa tagtatttgc tatagttgct ataggtgttt ctgtaaaaat cattaaaact
     4561 gttgctagtt tagcttttaa aatagcgttg atattgctaa taattttatt tgtatataga
     4621 atgtttttgt aaacatttaa aaataagttt tttaacacaa tagagaagta aaatagatta
     4681 tctttaatgt aaatttttta ttaaagacac tctattttac ttctctatta gcttctttaa
     4741 attaaatttt tattatatct tcccataata taaacatctt caaattttcc atctctgatt
     4801 acagcatatt ttttagttcc ctctatttca aaacctagct ttttatataa atttaatccc
     4861 ttttcattat ccacaattac ttctaatcct actctagtta acataatcca attatctgct
     4921 atatctaaaa gttgttccat aagcttctta cctactcctc ttccctgata attctctgca
     4981 acacttattc ctatttctgc gctatgtctc aatcgtggag aagaatttac atgtaaccca
     5041 gcaaaaccta ttacatcttt ttctccatta ttattagttt cagctacaaa catataatca
     5101 ttttctccca aagaagctaa catagcttca gttttatcga ctctgtcact cattaatgcc
     5161 aaagtatttc tcataacact gtatgttctt ctaatttcat taaaagcttg tgcatcttct
     5221 cttcttatag gtcttattgt tatatctata ggcttactca tattggattc cccctcaaac
     5281 ataatcttta tttttaatta aaattttaca tctttatttt atactgttca aatctatgta
     5341 tgcttctacc tgaggtacat atatcatatt atcttttata tgtgtacttc caaattcttt
     5401 taattctgta ccttctttat ccttagttat tttatatcta atggattcca taccttcaac
     5461 tacttcagca tgaaaaaaca taccttcata ttgtttaaat ctaaaattaa aattgtcttt
     5521 agtaagtttt attatcatat ttttatctac tccttatata attgatattt ttcttattat
     5581 tagttgtata tatactgctt attcatatta atatattata acttatcatt tatttagtat
     5641 aaagttataa atagatatat ttatacttat tttacaatct ttcttcaatt gatatataat
     5701 atgtttatat aaactaatgt atgtgaggtg aaatatgagc aaaaaaacaa aatatttttc
     5761 tttaattctc tttcctattg ctttgctttt aaattttata gccagtaaag ttccaaatat
     5821 tgttgaaaaa tattactccc aattcataga taaaataatt gtacaaatat tgagtaaagt
     5881 ttctggaatt ttcccatttt ctctctatga gattacaatg tacataatag ttatatctac
     5941 cttcttattt ttatgttgta ctttatctac aatttttaat aagaaaaaaa aattaaacat
     6001 atatttaaaa aattccattt taaatatatt atccattgta tcaatagtct actttctttt
     6061 tgtagtgctt tggggaataa actataatag gattccacta gaaaccacac ttataaataa
     6121 ctataattta aaaaacaata cctctatcca atcaaaacaa tatagtgtta aagagcttac
     6181 taaattatat aaattccttg taattaaagc aaatgaaact agaaaactca cattacaaga
     6241 taaaaatggt attgtaaaat ctaatacaga ttataatggt gtcataaata gagctcaact
     6301 aggatacgat aatatcttgg atatattacc tggtgttagt gatagttatt ctaagcctaa
     6361 gtatattatt tcatcaaatc taatgtgcta tacaggaata actggtatat actttccttt
     6421 tacaggtgag gcaaatgtaa atatagcaat acctgactta tatattcctt gtacagttga
     6481 acatgaaatg gcacatcaaa ggggatttgc tagtgaagat gaagctaatt ttatagcgta
     6541 tcttacttct ataaaacacc ccaatattga ttttaactat tctggatata ttttagcttt
     6601 aaattatact gcttctgctt taagtaaggt tgattataat gcatatgttg atatttctgc
     6661 tggcatttca gattctgtcc gtagagattt aaaaaatgaa agtgaatttt ggcaaaaata
     6721 tgaaggtaaa attaatgaaa tttctaatga gtttaacaat tcttacttaa aagctaatgg
     6781 cgttactgaa ggtactcaga gttatggtaa aatggttgat ttattgctaa cttattatga
     6841 attatatcca tataactaaa cactacaatt caaaatttta ttcatataca gctgatatta
     6901 aatcagctgt tttctattta aataaattat ttaacttcaa gcaatcttat ataatttgat
     6961 agtacttata ctatctgtat aacatttcaa ttctaactat tttagttaaa acatttctat
     7021 tatctttttt aataaatgtt ttaactaaaa tttttaaaac ataaaacttc cacattattt
     7081 cactatctat ataaaaatct aaaaattcac atataaaatt acataactaa aaaatcataa
     7141 atttatagaa aacatatatt caaaataaaa aagcatgtat caaaacttga cacatgctta
     7201 taatcttatg tatatatatc attttgtcac aataaaaatt tacttgtaac actgatattt
     7261 ttaaacaaaa tagtccttat ttaattaaat taacattaat taaattcgta ctcattttgc
     7321 tatcattaaa cattacttat agtaattttc taatacacta tttgaaactt ccttcccttg
     7381 aaaacttttc aaccaattct catatgctga gtttttccat acactaccat cattaaatgt
     7441 tacttgtttt acacaagata taatgtactt tacaccatgc tctccagttg attgtttcca
     7501 tttatcaaac aatagaaagg catcattttg attcttacct ggtgctattg cattttgctt
     7561 tgcatattct ttctccattt gtttccattc tttcaaccaa ttttctaccc atgccttgcc
     7621 ttgttgtttt tcaaacatac ttattatatc ctgtggtggt gattgttgca tttttctata
     7681 agtatgtgaa tatgattttg gagacacagg agatatgcct gtaacaatac catagtctac
     7741 accagctggg ctaaatccaa cacttcctac ttcgccatct gctgccacat tttgagcatc
     7801 ccaatatagc tcaagtggtt ttccattttt atcatatgct aagcatgtaa tttcataatc
     7861 cataattgcc ttttcactgc tatttgttaa gcttataaca ttgctaggcc aatgatatgg
     7921 agtatcatca tactgtattt tgctattatt tataaataat ggaatattct cattataaac
     7981 aagaccttta ctactttgaa tatctgatac cttctttgtg acagtactta ccgtatttgc
     8041 taagtataca tttgtttttt ctgaattggt cgtactaaga gaaaatgttg ttgtcgttcc
     8101 agtaatcagc aaagcaacaa ctaaaatagg taagctagat atttttttat atctcacaat
     8161 tcttcacctc tttttctatt aacttataat aaaacaattt accaaaacat taaaatctat
     8221 gttaacataa tttaccaact gttctagtaa ctatatccta tcatagttac tatcaagttt
     8281 caatactatt gatggaaatt ttcactatat taaatctttt tttaatttta taaatttaca
     8341 atatgattta actcatacat atatagtcaa attagaatat aagtttctta catttaatga
     8401 agtttataaa ataaaaaatg actagataat taaatactgt aaataaaaac ttattacaaa
     8461 agtatttaaa aatctagtca tttaatatat atatgaattg tatactataa tttttcaatc
     8521 tcttctagca actcatcaac taatttgtta ccatcaatca ttctaactat ttcacccttt
     8581 ttaaataaaa gaccttcacc tttaccccca gcaataccta tatctgcttc tctggcttca
     8641 ccaggtccat ttacagcaca gcccataatt gcaacagtga tatttttttc catactacca
     8701 atcttttctt caacctcatt tacaacacta attaagtcta tattacatct accacatgta
     8761 gggcatgata taactttaat cttatcattt aaaagtccca aacttcttaa aatctgttta
     8821 ccaacaataa cctcttcaac agggtcgcct gttaaagata ttctaacagt atctccaatt
     8881 cctttaagaa gaagtgctcc tacccctata gaagacttta ttgttccttt gtgaacactt
     8941 ccagattctg taattccaat atgaagagga taatcaactt tttttgatat taattcatat
     9001 gcatcaacag ttttataaat atcggatgat tttagagata taactatatt atgaaaatct
     9061 aagtcttcaa gaatcttaat atgtcccatt gcactttcaa ctaatgcttc agcagttgca
     9121 gagccatact tttttaaaag ttctttctct aaagaacctc cattaactcc aattctaatt
     9181 tttaaatttc tttccttgca tttctcaact accatcttaa ctctctctat acttccaata
     9241 ttaccaggat ttattcttat tccatccaca ccttgttcta tagcttcaag ggctagctta
     9301 taatcgaaat gtatatctgc aataattggt atatttacac tagactttat ctttgctata
     9361 tttttagcag ctttaatatc aggaacagca actctaacta tatcacaacc agcttcctct
     9421 aatctcttta tttgagctat tgtagcatct gcatctcttg tatcagtatt tgtcatagat
     9481 tgtatactta taggattttc tcctcctatt tttacagctc ctacacttac ttctctcgtc
     9541 tttcttctct cgtaactcat attccacctc tatatattat tttatattga atctctttaa
     9601 atattaaaat attataacct acatgtatat tataaattaa aaagctatct agttaaataa
     9661 gaataaattt catatataaa aattaattta ttcttattta aaactacata gcctattttc
     9721 ttaaaatctc taaaaaacta aatcattgaa ataatcttag tatatcttta tatgtgataa
     9781 aaagcataaa taacattaaa gctccaaaac caacaacatt tatcatgcct tctttatttg
     9841 ggtcgagttt tttaccacct ctaacagctt ctagtaacaa cataagtatt ctccatccat
     9901 caagtgctgg tattggtaat agatttacta tacctaaatt taagcttatg acagctgcta
     9961 aatacactac atttatgatg cctgtacgag cagcatctga taccatacca ataacaccta
    10021 ctggtcctgc tacagcattg ccaatccctc ctggtacacg tcctgtaatc atttgaccta
    10081 gaaatgttag catctgtttt gtcatatcta cagtagtttt acatgcattt actattgacc
    10141 ctaaaaagtc tttttctctt tgtggttgaa ttcctatttc atactttcca ttgttatttt
    10201 taggtgttac atttacaact ttatcacttc catctctagt tatactaagt tttaattctc
    10261 caccagaaga agtgtttatt atatttgcaa cttcatccca agtcttaaca gaattaccat
    10321 taattttatt tatcttatct cctacttgaa gccctacagc ctgtgcagga gtatctggca
    10381 ttatctttcc taatttagtc gttggagaac ctatatacaa aaatactgga actaaaagta
    10441 ttgctgctaa tattatattg aatattggtc ctgctacaat agtactaaat ctttgtaata
    10501 tagacttgtt tccaaaagaa tttggactta tttgttcttc atcttcacct tccatgctga
    10561 catatccacc aattggaagt gctcttatag aatactctgt atcttttttt acactatata
    10621 tcttaggccc catacctata gaaaactcat ttactttgat accacttctt tttgcaaata
    10681 taaaatgtcc taactcatgt attaatacaa ttatactaaa tagtattaat gcggctatta
    10741 tagtcataaa ttcaccttcc tattttttta tcagatttgc aacataagct ctactccatc
    10801 tatctgtttc taagatttct tctaatgttg gttcacttat gctactatga acttctaaag
    10861 tcttctctat gtaatatggt atgtcataga aaccaatttt atcctctaaa aattcactta
    10921 ctaaaacttc atttgctgaa tttaaaacag aagaataagt tcctcccatt tttaaacact
    10981 catatgccaa ttttaaacat ggaaaagttt ctaaatctgg ttcttcaaaa gttaaagtac
    11041 taaattttga aaaattcatt ctttcaaagc tactttccat tctatctgga tatgtaagag
    11101 catactgtat tggtagtctc atatctggac atcctaattg agcaattatt gagctatctg
    11161 tatactgcac cattgaatga attatgcttt gtgggtgaac tactacatct atattttctt
    11221 gttctactcc aaataaccat ctagcttcta tcacttcaag tcctttattc ataagcgttg
    11281 aagaatcaat acttattttt cttcccatac tccaatttgg atgcttcaaa gcttcatttt
    11341 tagttatatt tgcaagttct ccctttttct ttcctctaaa tggaccacca gatgctgtaa
    11401 gtattatctt ttctatattt tttttatttt ctccatttag acattgaaat atagcactat
    11461 gttcactatc aacaggaaga atatttacat tgtatttttc tgcttctttc attacaagtt
    11521 caccagcagt taccaacgtt tctttattcg ctaatgctat gtctattccc ttctttatag
    11581 cacaaagagt tgggactaat cctatcatac ctactacagc agttaaaagg acatccactt
    11641 catcaagtga tgatattatt tttaatcctt ccattcctgc taaaacttct atttctatat
    11701 ttttaggcaa catttctttt aattttaatg ctttattttc ctcaaaaact gttacatatt
    11761 taggtttaaa ctctactatc tgttctagca ataattcaat attactgtta gctgatattg
    11821 caactatctc aaacttatct ctatgttctc taaccacatc caaagtttgt ttacctatag
    11881 agcctgtaga acctaaaatt gatatctttt tcatataata atcctttcta ataataaata
    11941 taccctgtca aaacattata acactactaa aacaattatt tttactattc atagaaattt
    12001 catatattat tccacagatt aaaattaatc tattattttt tgtattttgt ggaatattat
    12061 aaaatattct agtattatta aatgcccttt tttatttcca caacatcttc tattttacag
    12121 tctagtgcta aacatatctt ctccattgta tccatagaaa cagatttatc atttcctagt
    12181 ctgcctaaaa cagaaatact tatacctgat atatttacaa attccatttt ctttattttt
    12241 ctgtcaatta acaatttcca taatggagcg tatgaaatca tgttatcacc tcgcaaaata
    12301 tattctatca cattttatct attttggaaa attttcatct atttagataa ataatcacct
    12361 gcttaagtgt caaataattt tatccttcta atatgaaaat ttaatactat actaaatgat
    12421 tggtatataa cacatatgat gcattattat taacatataa aaatttttat tggtgctaaa
    12481 ttaaagtaat ata
//
LOCUS       DAMJZE010000116        12419 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJZE010000116 DAMJZE010000000
VERSION     DAMJZE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.117"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..206)
                     /locus_tag="PC541_002661"
     CDS             complement(<1..206)
                     /locus_tag="PC541_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2840112.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQI"
     gene            complement(220..1122)
                     /locus_tag="PC541_002662"
     CDS             complement(220..1122)
                     /locus_tag="PC541_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2840113.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(1317..2492)
                     /locus_tag="PC541_002663"
     CDS             complement(1317..2492)
                     /locus_tag="PC541_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2840114.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(2579..3424)
                     /locus_tag="PC541_002664"
     CDS             complement(2579..3424)
                     /locus_tag="PC541_002664"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2840115.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(3522..4319)
                     /locus_tag="PC541_002665"
     CDS             complement(3522..4319)
                     /locus_tag="PC541_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2840116.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(4338..5348)
                     /locus_tag="PC541_002666"
     CDS             complement(4338..5348)
                     /locus_tag="PC541_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2840117.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(5367..6149)
                     /locus_tag="PC541_002667"
     CDS             complement(5367..6149)
                     /locus_tag="PC541_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2840118.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(6208..7344)
                     /locus_tag="PC541_002668"
     CDS             complement(6208..7344)
                     /locus_tag="PC541_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2840119.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(8053..8520)
                     /locus_tag="PC541_002669"
     CDS             complement(8053..8520)
                     /locus_tag="PC541_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2840120.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(8599..9141)
                     /locus_tag="PC541_002670"
     CDS             complement(8599..9141)
                     /locus_tag="PC541_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840121.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(9193..9879)
                     /locus_tag="PC541_002671"
     CDS             complement(9193..9879)
                     /locus_tag="PC541_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2840122.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(9879..10739)
                     /locus_tag="PC541_002672"
     CDS             complement(9879..10739)
                     /locus_tag="PC541_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840123.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            10874..11674
                     /locus_tag="PC541_002673"
     CDS             10874..11674
                     /locus_tag="PC541_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2840124.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            11699..12268
                     /gene="yfcE"
                     /locus_tag="PC541_002674"
     CDS             11699..12268
                     /gene="yfcE"
                     /locus_tag="PC541_002674"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2840125.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         3938 a         2273 c         1375 g         4833 t
ORIGIN      
        1 attatttgtt cacaacagtt tttaattaaa ttgtcattat aatctttaaa aacacctttt
       61 cttaaatcct catcttttgc aattcttttt atagctttct ggaaaaaacc atccacttct
      121 tgattaactt tgccttcaaa gtcatttaat attggaacat cactattttt gtccaaaaca
      181 tgtattataa atttatgtat tatcatatat atcaccccat tatcttaatt tcgatattaa
      241 ctctttcata tcctctggta tatctgcttt aaatttcaat ttttcttttg ttcttggttg
      301 ttcaaactct agcttatatg catgaagtgc ttgcctattt attagctctt catctacata
      361 accatacaac tcatcaccta ttattccatg acctatatga gccatatgta ctcttatttg
      421 atgtgttcta ccagtttcta agcttacttc taatacagtt gcattttcta gtctttctat
      481 tactttatag tgagtaacag atgactgtcc tctttcatct attatcctct ttattgaatc
      541 ttctgtaggt ctataaattg gctcatctat agttccctca ttttctttta taattccatc
      601 aactactgtt atatactttt tttcaacttt attttcactc attgctgtag acaaagtatg
      661 atgtgcataa gcattttttg ctactataac taaccctgaa gtattcatat ctaacctatt
      721 tacaaatctt atttttacgt tttctttttg attatctata tagtaactaa taccatttgc
      781 tattgtttta tcatagtggc tttttgttgg gtgaactacc ataaacggag gtttattcac
      841 cataattata tcaaaatcat cataaattat ctgtaaattt aaatcttgag gttcaaaatt
      901 tgctttttcc tcgtctattt ttacttctat taaatcacca ctatacacct ttaaactcgg
      961 ttttttatat actccattta ctaaaacact cttttccctc ttcatttttg ataatgacct
     1021 aactgagaaa tttagcttgt ccaataaaac ttcttttaaa gtcatttctt cttcatttgt
     1081 atacgatata agattatatc tttgattctc ttttttaaac aaacttttac ctactttctt
     1141 ttatatcttc ctacttttta tatcttcaaa atcatgatat tatatatttt acactatttt
     1201 tgtttaatag ataataatta tttttaacta taaaaacctg taagaataac aaatagttat
     1261 tcttacaggt taaattcata attaaatatt taatttgtaa atattataat ctaaaactat
     1321 ctcttaacta ttaaagtagt tcccattcct ccacctatac aaagtgtagc aagaccagtt
     1381 ttagcatctc ttctcttcat ttcatataaa agtgtagtaa gtattcttgc tcctgagcat
     1441 cctattggat gtcctatagc tattgctcca ccattaacat taactttatt catatctata
     1501 tttaagtctc ttattacagc tacagattgg gcagcaaatg cctcattagc ttcaactaaa
     1561 tctatatctt caatagtcat attagcagct tctaaagctt ttttagttgc tggaactggt
     1621 ccatatccca ttattttagg gtcaacacca gctgttccat aagaaactat agttgcaaga
     1681 ggctctattc ctagttcttc agctttttct ttagccatta ctactagcat agcagcacca
     1741 tcatttattc ctgatgcatt accagcagta actgttccat cttttttaaa tgcaggtctt
     1801 aacttagcaa gtttctccat tgtagtgcca ggcttaatat attcatcttt atctactaca
     1861 gtgtcacctt ttcttccttt tataacaaca ggaactattt cttcatcaaa ttttccttca
     1921 gcttgagctt tttcagcttt attttgactt gcaagagcta attcatcttg ttcttctcta
     1981 gttatattcc attgctctgc tatgttttca gcagtaatac ccatgtgata gttattaaat
     2041 atgtctgata atccatcttt tatcattgaa tcaacaaaag cagcatcacc cattcttgca
     2101 ccatatctcg cacttggtac taaataagga gacatactca tgttttcagc tccaccaact
     2161 aacattatat cagcatcacc taatgctata agttgagatg ccattgaaac agatcttaat
     2221 ccagaaccac aaactatatt tatagtcata gctggttttt ctactggtat tcctgctcct
     2281 aatgctattt gtcttgctat attttgtcca agacctgctg taagtactcc ccctaaaaga
     2341 gattcatcta tcatatctgg agttatgtta gctcttttta tagcttcttt agctgctgtt
     2401 acccctaact ctaccgctga aactgattta aatgctcctc caaaacttcc tactgctgtt
     2461 ctagctgcac tggcaattac tacttctctc atattcatta cccccattaa attagatttt
     2521 tcatatatcc gaaattgttt tttaaacaat tttatttaaa cttttaattc ccaaattctt
     2581 atttattata atcatagaat cctatcttag tttttcttcc taattgatta gctctaacca
     2641 ttttagctaa aagtggatga ggtctatatt tagtatctcc aaattcagta tataaaacgt
     2701 tcattatagc taaaacaaca tctaatccga ttaaatcacc taatgctagt ggtcccattg
     2761 gatggtttgc tcctaatttc atagcttcat ctatttcttc tttacttgca acaccatctg
     2821 catatatacc aacagcttca tttatcatag gtataagtat tctatttact acaaatccag
     2881 gagattcaga tacatctact ggtactttat tgatactctt agataattca aatactgtat
     2941 caaaagtaac ttttgatgtt aactgaccac ttataacttc aactaatttc atcataggaa
     3001 ctggattaaa gaaatgcatt cctataactt tatctggtct cttagtagaa gaagctattt
     3061 ctgttataga taatgatgaa gtatttgttg ccaagatagt atcttcttta cataattcat
     3121 ctagtaactt gaaaacatct ttctttatat tcatgtcttc tacagatgct tctattatta
     3181 aatccatatc ttttaaatct tcatagttag tagttgaact aacatgactt aatatttctg
     3241 cttttgtagc ttcatccatt tttcccttag taactaactt agttaaattt ttatctaata
     3301 aagctaaaca tttatctata gcaccttgag ttctactctt taaacataca tcatgtccac
     3361 aacttgcaaa agtttgtaca ataccacttc ccatagttcc actacctatt acagctaatt
     3421 tcatagttat aacctcctgt tattttagta tttatattct gttaactaga ttaaaatact
     3481 atttaatcta gttaacagca aatatatttt taattagatt tttacccttt tataaagtta
     3541 gcttctctct tttcaacgaa agctgacatt ccttcttttt ggtctttagt tgaaaaacaa
     3601 agaccaaata aattagattc tatatctatt ccagtattta tatcagtttg agcaccaagt
     3661 tgtattgctt ctttagagta tctaactgca agctgagcat ttttagctat tatcttagct
     3721 aatttctcaa cttcttctat taaaatgtct ggctcaacga ctctatttac tagccctatt
     3781 ttttcagctt catcagcttt tataacttga cctgtaaaga ttaattcttt tgcttttgcc
     3841 attccaacca atcttgtaag tctttgagtt cctccatatc ctggagttat tccaagagtt
     3901 acttctggct gaccaaattt agctttagca gatgcaattc ttatatcaca tgccattgca
     3961 agttcacatc ctccacctaa agcaaatccg tttacagcag ctatcactac ttttttacta
     4021 ttttctattt ctccaaaagc ttttgctcct aagatactaa aatctttagc agctacagca
     4081 tctaaatctt tcatgtatgc aatatctgct ccagctacaa atgcctttcc ttcccctgtc
     4141 aatattacaa catcaatagt ttcatcatta ttaatatcta caaatacttc ataaagttct
     4201 tctaaagtct ttgaatttat tgcattaagg gctttaggtc tattcatttt cactgtacat
     4261 atatttccat ctacttcaac agctacattc tcataaactt taacatcact tgtactcata
     4321 ataccctccc caagaaatta gttagctaaa acttcacctt tttcttttgc aacacttaac
     4381 tgactgataa gttctggaag cactttatga acatctccaa ctataccaac atcagcatat
     4441 ttaaatattg gagcttctgg atttttattt atagcaacta taaactcagc atcttccata
     4501 ccagctatat gttgtattgc tccagatata ccacatgcta tataaaggtc tggtcttaca
     4561 gttttaccag tttgaccaac ttgtcttgct ttatctaacc aacctgcatc tatagtggca
     4621 cgagaaccag aaacttctcc acctataatt tcagctaatt cataaagtat gtctaagttt
     4681 tcttttccac ccattccacg tccagcagaa actaatatct tagcatcttc tattttaact
     4741 tgttttttag cttcttttat tacttgtaca acttgaacta atttatcagc atcattaaat
     4801 tctaccttga aacggttaat tacagcttct ttagtttcat caggttcatt tttcttcata
     4861 acccctggtc taactgtaga catttgaggt ctgaaatctt tacaaactat tgttgccatt
     4921 atatttccac caaaggcagg tcttgtcatt aataataatt ttgtatcttc agctactgca
     4981 agacctgtac agtcagcagt aagacctgta tgtattctag cagaaactct aggtgctaaa
     5041 tctctaccta ttgaagttgc accaaataat acaactatag ggtcagctgc ttttattgct
     5101 tcataagctg cttttgtata tggttcagtt gtatacactg ctaaagcttc atcatctact
     5161 actattacct catctgcacc atagtgtgct aatgtatcta ttaaaccttc taccttacta
     5221 cctaaaagta atgcagaaac ttttgtatca taatcttttg ctatttctgt agcctttcct
     5281 agtaattcta aagaaacagt ttgaattaca ttttctcttt gttctattac tactaaaacg
     5341 ttacccattc tttatccccc cttaaattat atgatatact tctcttttaa tttatctatg
     5401 ataattccag ctgatgtttt cgcatcttca ttgtatattg taccagctgg tttaactgat
     5461 tttgtaaatg atttaaatac actagttgga gaacctttaa gacctaaatt agaagggtca
     5521 acttctatat cttttacagt ccatgtttct actacatcat tttcgaaagc atcatatatt
     5581 cttccaactt tcatgtatct tggtgtgttc atatctttaa gagttgttat aaggcatggc
     5641 attttaactt ttaagtcatg gcaacaatct tcaaattgtc tttttactaa tacatattca
     5701 ccttcagttt ttatttcttc agcatatgtt attgatggaa gatttaaatg ttcagctatt
     5761 tgaggtccaa cttgtgcagt atctccatct atcgcctgtc ttccagctat tataatatca
     5821 aaatctatat tttttaatgc tcctgctaat gctgatgaag ttgcccaagt atcagcacct
     5881 gcaaatgctc tatctgttaa taatatacct ctatctgcac ccattgctaa agcttctttt
     5941 aaagccatat ctgcttgagg aggtcccatt gttataacag ttacatgagc acccatttct
     6001 tcttttaatt ttatagcttc ttctaaacct gctttatcat cagggtttat tatacttggt
     6061 actccatctc taattaaagt acctgtatta ggatctagtt taacttctgt tgtatctgga
     6121 acttgtttta tacaaacgac tatattcata aatccttcct ccatatctat tttaatatcg
     6181 acctattgtg tatattttaa tatttatcta ttttaataat tttcctgaaa taaccattct
     6241 ttgaacttca ctagttcctt catatatttc agttatctta gcatctctca tcattctttc
     6301 tactggatag tcacgagtgt atccatatcc tccatgaagt tgtacagctt ttgtagtaac
     6361 ttccatagct gtttcagctg caaataattt tgccattgct gcttctactc cataaggttt
     6421 tcctaagtct ttatttatag ctgcttgata tacaaggtgt ctagccgctt gtaccttaac
     6481 ttccatatca gctaattgga attgtgtatt ttggaatttt gataatggtc taccaaattg
     6541 tactctttct tttacatatt taacagtttc atcaagagca ccttgtgcta aacctaaagc
     6601 ttgtgcagct ataccaattc taccaccatc aagagtagac attgctatct taaatccttg
     6661 accttctttt ccaagtaaat tttctttagg tattctgcaa tcctcaaata ttaattcact
     6721 cgtagctgaa cctcttatac ccattttctt ttctttaact ccaaagctaa acccaggagt
     6781 tcctttttca actataaatg ctgatattcc tttgttcccc ttagatttat cagtcattgc
     6841 cattactaca tatatgtcac cagctattgc atttgttata aatatttttg agccattaag
     6901 tatgtattca tccccgtcta aaacagcagt tgtttgttgg ccagacgcat ctgtaccagc
     6961 attaggctca gtaagaccaa atgctcctaa tttttctcca cttgctaatg gtcttaagaa
     7021 tttttgtttt tgttcttcat taccatattg atatataggc catgagccaa gagatgtatg
     7081 agctgataat ataactcctg tagtaccaca aactctagac aattcttcaa ctgccattat
     7141 atatcctaca gtgtctccac cttctccacc atattctttt ggatatggta tacccatcat
     7201 tcctgctttt gccatttttt ccactgtttc ataaggaaat ctttcttctt catcaagttc
     7261 tgttgctaaa ggtttaactt cattttcagc gaagcttaca tatagctctt taagcatctg
     7321 atatttttta gaatttaaat ccataatagc ctcctcagta ttttataaat attttatttt
     7381 catttattaa ttaaaatctc ggcaacatct ataataaggt atttgaatat acgtcgagaa
     7441 atttataacc ttaatcaaac tacgctctat atttatatgt gattatacta accaattatg
     7501 acattaattt aatcataaat tgttaattta aaatcaacta tacttaaaaa aaatctcgct
     7561 ttttggaatt ttgtgaaatt tcaagttaga ataagatttc tttcacactg gttagagata
     7621 aaataatttg tttacgatat ttatattaag gtatattaaa cattaaatat tgtctgtaca
     7681 tctgtttttt agccatgtac agaagatttt tatcgtataa aaattatttt aatagagcta
     7741 aataatcata tttttatctg actatttttt atttttaaat atcttacatc tataatagta
     7801 tcactcaaag gataattttt caataatttc taattatttt tttaacaata tttattacat
     7861 tttttccact tttttgtcat ttttttattg tttcgaaaaa aaatcagaat tttttaactt
     7921 tattttggat tattagttta aattagattt tcctcttaga tttataaata tttacacaaa
     7981 aaaactacct ctgtatagat tgtatacaga ggtagttttt tactaattcc tactatagtt
     8041 tactatattt ttttaccaat tcttacaaac atctacccat ttttcacact tagaaaactt
     8101 ctccatttct tcacatgtaa gttctattgc agagttacta ctgccacatg caggaaatac
     8161 tgtatcaaat cttttcattg aatcatctaa atagactttt atagaatttt ttaaaccaaa
     8221 tggacagact ccaccaatag catgtccagt aaactccaag acttcttctg gtgtcaacat
     8281 tttagcctta caattaaatt cagccttata cttcttatta tcaatcttag catcaccagc
     8341 tgtcacaatt aaaattgcat catctcctat tttaaatgag agtgtcttag ctattcttgc
     8401 tggaataacc ccagctgctt cagctgcaag ttccacagta gcactagatt gttcaaattc
     8461 taatatactg tcttccttgc caaattgttt aaaatactct cttacattac ttattgacat
     8521 taaatacccc tcctagtctg aacttttata aatttcatat tattcttaat tatatctata
     8581 attgatttat ttttcaaatt attccataat attagcattc catgtatcat tattttttcc
     8641 aactactaga taaagtcctt ttgccattgg aatattgaca atatattctc ctttatactc
     8701 tacactcata ccagattcat taaagtacct ataatcaaaa tttagtcctt ttggaattac
     8761 aaaatcaaca taaccttgat tatttggttt tattccatat ttcagagttc tttcagttat
     8821 agaatttttt tcattaaata aatctacatt tacatatgca gaacacttat tcattccctt
     8881 tttattttcc acacaatttt caaacttcat tttagcactg tttaatttat aactatattt
     8941 actattattg cttattttaa atcttacaat agtaccaaca tctctacttc tcctgctatc
     9001 aaaatcaaaa acaatttcct ctaaatgatt tatttcataa tctttgacac ttaccatatc
     9061 tctatatact aatttattta tagctatgaa tagtgtaaaa atccccaaaa taaaaaatga
     9121 aacttttata aacttcttca tttttagtct ccttatattt tgtgctattg tgctataata
     9181 ttaatcaaaa ttttaataat taaacttatt cttttttgaa atcttaatag aaaaatacat
     9241 tgatattata aaaattatta ttgttaaaaa tgtcatacta tataaattta tatacttagt
     9301 attagctata tcagttacat aatttgaaag atatactcct ccaactttac tataattaaa
     9361 atgctttact tgatatacaa tcatactaat catcttcaaa agacttccat atacaaagta
     9421 aattaaaata cttactataa aaccacaaac tttcattttt ccatatccaa acttaaaaat
     9481 taaaggcagt aaaatcgtca aaattgctaa aaatatattt acagaaaaaa atacatcatt
     9541 taaaaccata tttctaaaga acactagtga tgtcaattta cttagtaagg acacaattat
     9601 tgttacagaa attattaatc ctacacctaa taaatatttt gagtaaacta tatcttctat
     9661 actaactggc atactcctta taaaaatcct tgaatcattt tcataatcac actcaaatgt
     9721 atttataaat atcaaataac taacaacaat acttactgta taatatgaaa ttgaattaag
     9781 gaaaaaataa aacacaaaaa atagcaatat gtactttaaa ttacggatac catctgaact
     9841 aatccctatt attaaatctt ttcttattaa attgaacatc atctcacctc atttacataa
     9901 taatacatta gttcttctat agtaggattt ttgaccttat gcttatcaat ttctatcaat
     9961 aggcttttta ctttcaagct atctccaact ttaacaagtg cctcattata atatttatac
    10021 tctttcacac caactatgct ttctttaatt gcttctaact ctgctttatt tgccctaatt
    10081 atcttatatt cttgttctat taattctttc ttaacagtaa aaatctgatt tcctctattt
    10141 ataaaaacaa gataatcagc aattttaatt aaattatcag tattatgagt agaaattatt
    10201 acactactac ttccattttt cacaaaatct tgtaagtact gcattatttc agttcttaca
    10261 attgggtcta tcccagcagt tggttcatcc aaaatcaata acttagcatt gtgactaagt
    10321 gcacttgata acataagctt ttcagattga cctttagata aattttcaat atgcatattt
    10381 tcttctatat caaacttatt aaggtattca tcaaaccttt ctctatcaaa attcttataa
    10441 aataaagaaa ctatttttct aaattcctta acctttaaat gcggataaaa ttgtaaagaa
    10501 tcatatacaa aaccaatatt gtttttaaag cttattggag aattttcaat agtttcatta
    10561 caaagttcaa tttcaccttc atctgcatca ataatcccca ttataatttt tattaaagtc
    10621 gtttttcccg aaccatttgg tcctataatc ccaacaatat ccccttcctt taactctaag
    10681 tttatattat ttaacttgaa ttttcctaat ttttttgtga tattccttat gctaaacaca
    10741 tcttcacctc ttatgcattt ttttataccc acctatacat attttaacac attcactaca
    10801 aaggtcattt ataaatttat aaattaagaa ttataaaact atgttataat acaaagtatg
    10861 aagaggtgaa tatatgaaaa gaattataac tataaataca aaccaattaa ataagtccct
    10921 tgagactaaa gaactcttaa ctagaaaact tatcaatgct ggctttgaag tgtattcaga
    10981 tatttatcca gatacagagc ttattatatc aattggtggt gatggctctt ttttaagaac
    11041 agttagagat ttcgattttc ctgaaattcc cataatggga ataaatacag gtcatctggg
    11101 gttttttcct gatattttac cagataagat tgatagcttt attgaagctt atacaaaaaa
    11161 agattatatt attcaagaaa tgtcacttct taatgcggaa gtatatacaa ctactagtgg
    11221 ttctaatatg cttgctgtaa atgaggttgt tataagagga gacaagtcta ggactataca
    11281 cctaaattta agccttgata ataaacatat acaaaatttt agtggagatg gtatgattat
    11341 ttctacttcc acaggttcaa cagcctataa ctattctgct ggaggtagta ttgttgatat
    11401 aaatcttgaa cttatgcaga ttacaccttt acatccgata aataccaatg catataggtg
    11461 ctttacatca agtataatat gttcaaatga atctgttata aagattgcac cagaatatag
    11521 atttgaagat tctgtattga ttgtcgttga tggagttgag cacagattta gacaaatcga
    11581 aaatataaaa gttagtatat ctgatgcaaa aataaaatta cttagaatgt caaactatga
    11641 gttttggcat agagtatctg aaaaattttt atagactgta cttaggagga tttttaatat
    11701 gaaaatagga ataatgagtg atactcatgg tagcttactt tactttgaaa aagcgttaaa
    11761 tgtattatct gattgtgatg ttttattaca tggtggagat gtcttatatc atggacctag
    11821 aaatgatatt ccagaaggat ataatcctaa aaaatttatt gagacattaa ataaattaga
    11881 aaacattgtg attgtaaaag ggaactgtga tgcagatgtt gaccagatgg ttatagaaca
    11941 tcctattcag agtccttatg tcatgagcca atttggtgaa attagaatta tattgaatca
    12001 tggttatata gaatcagaag aagaaattat tgacaaagca aagaaaatgg gtggtgacat
    12061 attagtttta ggacatactc atgtaaaaaa gctatacatg gatgataatt taatagtaat
    12121 aaatccagga agtacttcta tcccaaaaga tggaagccat tctgtcgcaa ttatagatat
    12181 cataaaaact gatgatgagg atgagcttga acttgatata aacttaattg acatcaatac
    12241 tggaaatata ataaatatca atgattagta aatttttaat aaaaataaaa taagctttaa
    12301 tttattaaac tcataataaa aacaagtaaa ccattttatt tctatctcaa ttagaagttt
    12361 aagtacaata ccttaaactt ctaattaaat agaatctggt ttacttgttt tattatatg
//
LOCUS       DAMJZE010000117        12338 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJZE010000117 DAMJZE010000000
VERSION     DAMJZE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.118"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..321
                     /locus_tag="PC541_002675"
     CDS             <1..321
                     /locus_tag="PC541_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2840126.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="PC541_002676"
     CDS             337..1920
                     /locus_tag="PC541_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2840127.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="PC541_002677"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="PC541_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2840128.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="PC541_002678"
     CDS             3475..4827
                     /locus_tag="PC541_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2840129.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="PC541_002679"
     CDS             5019..6128
                     /locus_tag="PC541_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2840130.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="PC541_002680"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="PC541_002680"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2840131.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="PC541_002681"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="PC541_002681"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2840132.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="PC541_002682"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="PC541_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2840133.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4925 a         1425 c         2503 g         3485 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatct
//
LOCUS       DAMJZE010000118        12153 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJZE010000118 DAMJZE010000000
VERSION     DAMJZE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.119"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            107..2218
                     /locus_tag="PC541_002683"
     CDS             107..2218
                     /locus_tag="PC541_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2840134.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2416..3381)
                     /locus_tag="PC541_002684"
     CDS             complement(2416..3381)
                     /locus_tag="PC541_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2840135.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3666..4622)
                     /locus_tag="PC541_002685"
     CDS             complement(3666..4622)
                     /locus_tag="PC541_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2840136.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            4990..5778
                     /locus_tag="PC541_002686"
     CDS             4990..5778
                     /locus_tag="PC541_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2840137.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(5901..6371)
                     /locus_tag="PC541_002687"
     CDS             complement(5901..6371)
                     /locus_tag="PC541_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2840138.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            6510..7154
                     /gene="uppS"
                     /locus_tag="PC541_002688"
     CDS             6510..7154
                     /gene="uppS"
                     /locus_tag="PC541_002688"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2840139.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            7197..8102
                     /locus_tag="PC541_002689"
     CDS             7197..8102
                     /locus_tag="PC541_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2840140.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            8417..9964
                     /locus_tag="PC541_002690"
     CDS             8417..9964
                     /locus_tag="PC541_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2840141.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            9967..10365
                     /locus_tag="PC541_002691"
     CDS             9967..10365
                     /locus_tag="PC541_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2840142.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            10405..12138
                     /locus_tag="PC541_002692"
     CDS             10405..12138
                     /locus_tag="PC541_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2840143.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
BASE COUNT         4853 a         1317 c         1957 g         4026 t
ORIGIN      
        1 attttaacta tattattcaa tatttgacag ataatatgaa ttttagatat acttaataag
       61 aagtatattg tagaaaaaat aaatttaaaa aattaagggg gatgaaatga aaaaaataag
      121 tatattagta ttatcattga taatgacact tactatgtgt agtgtaagtt catttgctga
      181 ttcatcaaat gataaggaaa tgagagctgc ttggatatct acagtttata atttagactg
      241 gcctaagact aagaataatg aagccaagca aaaaaaggaa tacacagatt tattggacaa
      301 attaaaaagt gtaggaataa atacagcagt ggttcaagta agaccaaaat cagatgcttt
      361 atataaatca aatataaacc catggtctga atatcttacc ggtacacaag gtaaagaccc
      421 tggatatgac ccattaccat ttttaataga agaagcacat aaaagaggaa tggaatttca
      481 tgcatggttt aatccatata gaataactat ggcagatgag agcatagata aattgccagc
      541 aaaccatcca gcaaagaaaa atccaagctg ggtagttaaa catggaaata agtattacta
      601 cgacccaggt ttaccagaag ttagaaagta catagtggat agtatagcag aagtagttca
      661 aaattacgat atagatggag ttcattttga tgattacttc tatccaggag taagtttcaa
      721 tgatactgca acataccaaa aatatggaaa aggacaaaat aaggatgatt ggagaagaga
      781 aaatgtaaat actctactta gagatgtaaa agcgtctata aagagtataa agcctaatgt
      841 tgtatttggt gtaagcccag caggtatatg gagaaataag agtagtgacc caacaggttc
      901 agatacatca ggtaatgaaa gttatgtagg tacatatgca gatacaagag catggataaa
      961 acaaggattg attgactatg tagttccaca actttactgg ccaattggtc ttaaagcagc
     1021 agattattct aaattagtgg catggtgggc taatgaagta aaaggaacaa atgtagactt
     1081 atacatagga caaggtatat ataagcaagg tcaatcgagt tatggtggac aaaatatagc
     1141 aaaagaaata gtacaacaag taacattaaa tagaaagtat agtgaaataa aaggtagtat
     1201 gtatttctca gctaaagata tagcaaatag tacttctata caaaaggatt taaagagttt
     1261 atattctagt agtgaagaac cagttactcc accatctaat gttaaagtgg agaaattaag
     1321 aggcgacgaa agatatgaca cagcagtagc aataagtaaa aaaggttggg ctacaaattc
     1381 agatacagta gtattagtaa atgggtactc tatagttgat ggtataacat caacaccact
     1441 tgctacatca aatgatgctc ctatactatt ggtaaataag gataatatac ctacatccac
     1501 taagaatgag ttaaaaagac ttaatccaag taaagttatt ttaattggag gaaataactc
     1561 tattggagat aaagttgaga gtgagataaa agatacattg tcaaatgtat caataaatag
     1621 agttggtggt tcagatagat atagtacatc tttaatgata gcaaaagaat tagtaaaaac
     1681 taatccagta gaaaaactat atataacaag tggtacaggt gaagcagatt ctctttctat
     1741 agcttcaaaa gcaggagaag aaaagcaacc aatagtatta gtatcaaaag ataatgtaag
     1801 tgatgaagtt tacaattgga tatcagattt gaaagtaaaa gatgcttatt ttataggtgg
     1861 taatctaagt atatctgatt cagtaataaa taagctggat aaggtcatta caaatgatgt
     1921 atctaaaaat agaatagctg gagaaaatag agaagaaaca aatggaaaag taatacaaaa
     1981 attctatcca aatgctgaat attcatcaat gtttgtaagt aaaagtaatc aattggtaga
     2041 tgcattaaca tcaggtcctt tagcagctaa attaaaatca ccagtagtta tgcttggaaa
     2101 tagtgtaaca tctgctcaga aaactgcttt agagcctaag aaaactactc tagtatacga
     2161 agctggagat ggtataaatc aaaacacatt aaacactttc ttaaacttag ttaagtaata
     2221 aaaaagttaa tttaaaatac agagtatttt ataaatgtag attaaaatga aaattatcat
     2281 gataaattag aaaataaaaa agaactatat gcattttgta atcaagtgta cacagcaact
     2341 tgaaaataat tatgcgatat agttcttttt tagtaattaa tcttatactt tttaataaac
     2401 tttatgttga attttttatt gtttaaactc tatgtcatta aatataaaat cgtgtaatat
     2461 gtctaaagta tctgggtcaa atctaagaac ccatccagct tttccatata taccacctgt
     2521 actatgaatt tcatcatcaa taggaaattc aaattgctta atatttaagt cgcccatttt
     2581 taaaacttga gctcctaaag aaagtattgc attaggcttc atgttagttt taacatatgg
     2641 aagaactgca tctaataaac ctggatattt agtaacaggt aaagtttccg ttttcttgat
     2701 tatagcttgt ataatctgtc tctgtctgcc atctctagca aaggctgtat cgttgtgtct
     2761 gatacgtgca aaagatagtg cctgatatcc atttagagtc tgctttccag agtttctaat
     2821 gtattgtatg ctacctttat catcactttt attccattta taagtttcag gaataaattt
     2881 atttagttct cgtatttctc ctttttcaac agtaacctca acgccaccaa tagcatcaat
     2941 tatatacata aatgattcga aattaacaca agcataattt tgaatatcaa tattaaaatt
     3001 ctcttcaatt gtttgtatta ataaatcagc ttgtccataa gcataagcat gagtaagttt
     3061 ttgtttacca tgaccaggga tgtctacata gctatcacga gctaatgatg taagtttgat
     3121 atcattgtgt ttgttatcaa tagtaagtat catcatagca tctgaccttg aagaatcttc
     3181 atttggtcta gcatctgttc ccataagtag tatgttaatt atcccatctt catttttatg
     3241 gtcattgtta tccaataaat cactacttat gctcgagtca tgaatagcag ataatttata
     3301 ataaaaatat ccatagacac ttatagggaa tattactacc aagaaagcca gtagtataac
     3361 aaatttcttt aattttgaca aaagtacccc ttctttcttc tttttcctgt atatttttta
     3421 atagcataca cttaattata ttaaagtatt gagatatatt caatacttgt ttcagttaca
     3481 aaaaaaagac aatgatataa tttataagaa aatttatcga cctatatata tagttaaaag
     3541 tatatataat atatttttta aaagtaattt atattttaaa ataaaaacac accccataaa
     3601 tacaaagtaa tttgcattta agaggtatgt atttatcatc aaattattgg tttgttttgg
     3661 aatttctaat cttcaaccat aatatcttta aatatgaaat catgcattgc atctagttca
     3721 tattcctcaa aaggtattac atagcctgca ttacctattt ttctaccatt ttcagtagaa
     3781 agaggaaact ccatagattt caaatttaaa tttcctatac ttaaaagttc ttttcctaaa
     3841 gatattattt cattaggatt catattagtt tttacatacg gaagtattgt gttaactaga
     3901 ttaggatatt tagtgactgg taaatcctta acaccattta ttaaactctg tataacactt
     3961 ctttgtcttc tatctctttc atctgtacta tcattttttc ttatacgaga aaatgcaagt
     4021 gtctgatatc catttaattt ttgctcacca gaatttctta tatatttaag agaaccttta
     4081 ttgtcatttt tattccattt ataagtttca ggaataaatt tatttaattc tcttatttca
     4141 ctcttatcaa cagtaacagt aactccacct aaagcatcaa ctatatccat aaaagagaaa
     4201 aagtcaacta cggcgtagtt ttgtatatca agttggaagt tttcttctat agtttctatt
     4261 aataagttag cttgtccata tacgtaggca tgtgttaatt tttgttctcc acgtcctggt
     4321 atattaacat aagtatctct atttaatgaa gttagcttaa gacttttatt tttattatca
     4381 atagtaagta tcatcatggc atcagaacga gaaacttctt ctcctggtct tgcatctgta
     4441 cctaacaata atatgtttgt gatattttct tcgtttttat agtcattact actaagcgcc
     4501 tctgtattta tactatcatc ttgtatagtt tttaatttag taaaaacata tccatatata
     4561 ctaacaggaa atgctataac taaaagggct agaaatataa cgaacttttt gagtcctgac
     4621 aataatatcc cctactttct aaatttttta attacacata gttaattata ttaaaaattt
     4681 gttacataag caataaaaaa cacaataaat tagcataagt ttgtcataat ataattattt
     4741 ttatagcatt ttatagaaga aagtgtgtag ttattattga ttttattgga atataattgt
     4801 atgaagactt aaatttttat aaactgatgt gtatattgaa aaaaatattt tcctgaatgt
     4861 agttatagca tcttatgaaa ggtgcaaatt gtaatttaaa atttttagtg tataataaga
     4921 attgaaaatt caaaccgata aaaattaata aaaaataatt ataaattaat aatttggggg
     4981 aggaaatgta tgataaaatt aatagcaaca gatttagatg gaactttgtt agatgaaaag
     5041 agcgaaataa atccagagtt ttataaagta tttaaaaaat taagagagag agggataatg
     5101 ttttctgctg ctagtggcag gcagtatcaa aatcttataa aaaaatttga agatataaaa
     5161 gatgatatga tgtttatatc agaaaatgga acactagtag tatataaagg aaaagaaata
     5221 ttatcaaatc ctttaaataa agaattagta aatgaaatca tagagacaac aagaagtata
     5281 aaagggaaaa aaattgtaat gagtgggaaa aaatacgctt atatagaaag caaagatgaa
     5341 gcgtttatac aagaggtcag tacttattat gcaaagttta aaattgtaga agatttgacg
     5401 aaagtagaag gagacatatt gaagatagct gtatttgact tcaaaggtgc agaacataat
     5461 aataatatct actttgaaaa gttttctgat agagctcaag tatgtatttc tggagtggag
     5521 tggcttgatt taactgcaaa aggtgcaaat aaagggagtg ccataaaaaa agtacaaaaa
     5581 atgttagata taaaatatga agaaactatg gtatttggtg accagttaaa tgatgtggaa
     5641 atgatgaaaa gtgcctatca tagttatgcc atggaaaatg caaatgaaca tttgaaacaa
     5701 attgctagat ttagagcaaa aagaaatact gaaaatggcg ttgtagataa gataaaagag
     5761 gttataaaaa ttggatagtc atatgtatag atgaaaaaga ttattttgaa gaaatcaaaa
     5821 aaatatcaat gtaattttga taaataagtt tatttatgcg aaaattacat tgatatttaa
     5881 taaacatcaa taatagatat ttattttttt atgatattca gtattttgtt ttctatttga
     5941 gttgcatttt cagcagaaat taaataaaca tatctacctg ccttctcaat tatagtatta
     6001 tttgctattt cagaattgat tttttctcca tgtccttctc caaaaggtgc ttgtatcata
     6061 tcttttttat aattttctag agttttatag attttatcta cctcgtcagt ttttataacg
     6121 ataatatctt ctagagataa tttttcagaa gaattaatta caaaaccttg aattatgtct
     6181 tcctctacat cattaaaata gttaaaatcc tttgcatcaa agatttttga ttcctttata
     6241 agtaaatcag aagattttaa tgcttttttt atttttgatg tagaaacgtt tttgtaatca
     6301 ccacttgaag aacaactaac cattcctaat gataagatta tcgaaaaaaa caatattaaa
     6361 atttttttca tgataaacct ccaagttttt taaactctgt cttaaattag tttactacat
     6421 aattatataa aaataaatgt atttaatgtt acagttttga ttaataatgt aatattttga
     6481 taataatacc tattgagagg tgattagaaa tgagaatacc aaaacatgta ggaattatac
     6541 cggatggaaa tagaaggtgg gcaagtcaaa atggtatgac aaaagataaa ggatatgaaa
     6601 gtggaataga ccctggacta gagtcttata gattatgtag agatttaggc tgtgaagagg
     6661 tgacttttta tggatttaca actgataata caagacgtcc atcagtgcag acaaaagctt
     6721 ttacaaaagc atgtattgat gctgcaaata tattaataga tgaaggtgct gctttattag
     6781 ttgtaggaaa tacaaaatct ccaatgtttc cagaagaatt attaccatat actaatagaa
     6841 ttaaagccaa agaaggtcaa ataaaagcaa atcttctggt aaattatggt tggtattggg
     6901 acttaaataa tatatcagga gtagaaaatg taaataaaaa taatatacag gattacatac
     6961 agtcatttga tgtatccaga ctagacttga taatcagatg gggtggaaga aggagattaa
     7021 gtggtttcct tcctgtacaa tcaatttatg cagattttta tgttatagat aattattggc
     7081 cagattttaa gagtgacgat ataaaggacg catttagttg gtatgataca caagatgtca
     7141 ctttaggtgg ataattaaaa atattctgat ttaaggttgt agagtgtttt aaattattga
     7201 aaaaaaaact tcttgatggt aaaataacac taataatatg taaaagtgta aaaatttata
     7261 ccaagaaggg tgaagaaatg tcaaaaggca ataataacaa taattcaaga aacaaatcta
     7321 aaaagaccag tcatctgaat agaaaaaagc gaaaattaaa taagaaaaag ttagcagtgt
     7381 tgatttgctt tacagtttta tttttattta ttgcatttaa ggctactcaa ggtgtagtag
     7441 ctttggtaaa aagtatggat aaaagtaata aaactaatca acaacaaaat gttaactcag
     7501 aacaatttga ccttggtaat gaagaggaaa ataaaaagaa aaaatatact gtatttatag
     7561 acccaggtca tggaggcaat gataaaggaa ctgaaagcaa gaccagtaat agatatgaaa
     7621 aagacctaaa tctacaaatt gcaaaaaaat tagcaaataa attatctaaa caaaaggata
     7681 ttcaagtagt agtatccaga actgatgata catatatatc attaaaggat agagctatct
     7741 tagcaaataa tagttctgct gatgtgcttg tttctattca tttaaatgca gagaaaaatg
     7801 gtaatacagc aacaggtata gaaacttggt ataggaataa agctacagat ggttcaaaag
     7861 aacttgcaca gacagtacaa agtactatag tatcctatgt taaagttaga gataggggaa
     7921 tagttgaaaa taactttgaa gtacttagag aatcaaatat gcctgctata ttaatagagt
     7981 gtggtttttt aacaacacct tcagaggaac aaaaaataat aaatgaaaaa tatcaagacc
     8041 aacttgcgga aggaattgtt caaggtgtac tttcatacct agattctaaa gggaataagt
     8101 aagttaactt tactttaatg tgaggataaa tttaaaagtt taactgtgta aattgtatta
     8161 actttatttc gtaatgttgc aaacattgtt tgaatttaga aactatgaaa ttagaaatta
     8221 ttttatctat atctaatttc atagtttttt ttgcaaaaaa tttatagtta taaaatttta
     8281 aaaaataaat ttagtataat gtaatgtagt tatttatatg tgattttaac ttgcattgat
     8341 ttgctatcaa ttgtgcttta taattagtat atataaagtt taactgtaag ataatttaaa
     8401 aaatagggga atttatatga ataaaaatag catgacaaga atttacagtt taaataaaat
     8461 aatttttatt ataattggaa ttttaggaat agttttactc acaagacata ttgaggtaga
     8521 tattaaagtt ttaattttaa ttttattaac cttgtttagt ataaatgtat ttatttcata
     8581 tcttaaaatt aagttgtatg aggataaaat taatttagga ttgataaata attcagataa
     8641 gtttataaat attactaaag atgagaactt cctaaaacat gttcaaaatt atattatcag
     8701 taatgaaaaa gaaaactgtg taatggcttg ttttgattta tgtagactca aacttataaa
     8761 tgatatatat ggttatgagc taggggatga agtgttaaat aatattttaa gtaacttaaa
     8821 aaattatttt ggaaaagaag ctatttatgg aaagttaaaa agtgatgttt ttgtattaat
     8881 aataaagctt gaaaataggg aacagaagat tccttattta gtaaatctta ttaagaataa
     8941 aattgtgata ctaagtcaga ttgacagttt taaaatggat ataaatattg aggcttctat
     9001 tggaatttat aaatttaaca ataatgaaat tgatataaaa aaagctatag ataatgctga
     9061 tatggcaaga cttaaatcaa aaggactaaa gcacatagag tatgtagaat ttgataatgc
     9121 tatggaagaa gaaatacaat caattatgaa aatagagaga gatttattct tggctattaa
     9181 aaataaagag tttgtaattc actatcaacc taaagttgat tcaagtacag gtaacattat
     9241 aggttcagag gcattgataa gatggttgca cccatcatta ggcatggttg gaccaaataa
     9301 gtttatacct atagctgaaa aaaatggtct gataaacaat attggtagat ggctaattca
     9361 agaagttttt attaccatta acaagtggat ttcagaaggt ataaatgtct taccagtctc
     9421 aataaatctt tctagagtag aactatataa aaatgattta gttgattttt ttaaactgat
     9481 gtttaataca tataatattc caaaagaact tatagaatta gaaattacag aaacgacagc
     9541 actaagagat gtaaaattta taagtgaaag gctttatgag attaaatctt taggtatgaa
     9601 tatatcatta gatgattttg gagtaggtaa ttcaaatttt atcaacctta aggggatacc
     9661 actagatatt ataaaaattg ataggtcact tatattggat atagttacaa atacaaaaac
     9721 aaagtttatg gtgaaagcaa ttgttaacct ttcacatgat ttaaacgtaa ctgttatatg
     9781 tgaaggggta gaggatatgc atcaggtcaa agtcttaagt gaactgggat gtaatttcat
     9841 acaaggttat gtatttttta aacctttgga tgagatgaat tataaaaaac ttcttactga
     9901 cggaagttat ataaatctga aagatactct tttagataaa tgcatgacag ttgaggagga
     9961 gtaaggatga tttcagaaga tgtagattat tcaataagaa tcataagagc tatatttaat
    10021 aatggagaag ataaaccaat agatgctaaa atgatattaa aaagtgaaga tataccaata
    10081 ctaacactat acagactgct tagaaagctt tgtgcaaaca atattcttac ttttaacaaa
    10141 gaagatggca agacatacat gttaaatgtg tcattggata caataagtct aagaactatt
    10201 gtagaagtaa ttgatggaaa aatatatata aataaatgta cgagatatgt atctaattgc
    10261 agtaattgct taaaaggttg taaagtaaat cgtgaattgc tagaaataga aaaagaaatt
    10321 tataaacttt tatcgagaaa atctataaga caaatactag aataaaaaat tttcagctat
    10381 gataataatt ggaggttttc ggttatggat gatatatctc aagataattt tctactgtct
    10441 aaagagtatg agaatagctt agatgtagat actaaaaaag caagtggaat atattatact
    10501 cctaaaataa ttgtagatta tattgtaaaa aaaactttaa aaaatcacga tataattaaa
    10561 aatccatatc cacgaatact tgatatttcc tgtgggtgtg gaaattttct tttagaagta
    10621 tatgatattt tatatgactt atttgaggaa aatatatatg aattgaaaaa gaagtatgat
    10681 gaaaattatt ggactgttga taacatacac cgtcatatat taaactattg tatatatggt
    10741 gctgatattg atgaaaaagc tataagtata ttaaaagata gcttaacaaa taaaaaggta
    10801 gtaaatgatt tagatgaatc tgatataaag attaatctat tttgttgtga tagtcttaaa
    10861 aagaaatggc gatataagtt tgattacata gtgggtaatc caccctatat tggtcataaa
    10921 aaacttgaaa agaaatataa aaaatttctc ctagaaaaat atagtgaagt ttataaggat
    10981 aaagctgact tatatttttg tttttataaa aagattatag atattttaaa gcaaggaggg
    11041 attggcagtg ttataactcc aagatatttt ttagaaagtc tatcaggtaa agatttaaga
    11101 gaatatatta aatcaaatgt caatgttcaa gaaatagtgg attttttagg tgcaaatata
    11161 tttaaaaata taggggtatc tagttgtatt ctaacttttg ataagaaaaa aacaaaagag
    11221 acatatatag atgtatttaa aatcaaaaat gaggatatat gtataaataa atttgaaaca
    11281 ttggaagaac ttctaaagag tagcaaattt gaacatttta atataaatca gagattgcta
    11341 tctgacgaat ggatacttgt aaataaagat gatgaaactt tttataataa gatacaagaa
    11401 aaatgtaaat atagcttgga agatatagca ataagttttc aaggaataat tacagggtgt
    11461 gataaagcgt ttattttaag taaagatgat gtaaagttga accttgtaga tgataaattt
    11521 ttaaaatgtt ggataaaaag taagaatata aataaatata tagtagataa aagtgaatat
    11581 aggctaatat actcaaatga tatagataat gagaatacaa ataaaaggat actagatgaa
    11641 attatagggc tatataaaac aaaattagaa aacagaagag aatgtaaaag tggaataagg
    11701 aaatggtatg aacttcaatg gggtagagaa aagcttttct ttgaaagaaa aaagataatg
    11761 tatccttata aatcaaatga aaatagattt gctatagatt atgataataa tttttcaagt
    11821 gcagatgtat attctttttt tataaaagaa gagtatttag ataaattttc atatgaatat
    11881 ctagttggaa ttttaaattc cagtgtttat gataaatact ttaaaataac tgctaaaaaa
    11941 atgagtaaaa atatatatga ttactatcct aataaggtta tgaagataag gatttttaga
    12001 gataataatt atgaagagat agagaattta tcaaagcaaa taatatcaat tttattaaat
    12061 aagtctatag ataaaggaaa agtagaaaaa ttgcagataa aaatggataa cttgataatg
    12121 gattctttag gtatataagc agttgtaagt att
//
LOCUS       DAMJZE010000119        12003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJZE010000119 DAMJZE010000000
VERSION     DAMJZE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.121"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(236..1966)
                     /gene="lpdA"
                     /locus_tag="PC541_002693"
     CDS             complement(236..1966)
                     /gene="lpdA"
                     /locus_tag="PC541_002693"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2840144.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2133..3179)
                     /locus_tag="PC541_002694"
     CDS             complement(2133..3179)
                     /locus_tag="PC541_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2840145.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3192..4178)
                     /locus_tag="PC541_002695"
     CDS             complement(3192..4178)
                     /locus_tag="PC541_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2840146.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4203..5165)
                     /locus_tag="PC541_002696"
     CDS             complement(4203..5165)
                     /locus_tag="PC541_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2840147.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5203..6156)
                     /locus_tag="PC541_002697"
     CDS             complement(5203..6156)
                     /locus_tag="PC541_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2840148.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6601..7974)
                     /locus_tag="PC541_002698"
     CDS             complement(6601..7974)
                     /locus_tag="PC541_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2840149.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8071..9717)
                     /locus_tag="PC541_002699"
     CDS             complement(8071..9717)
                     /locus_tag="PC541_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2840150.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9739..10905)
                     /locus_tag="PC541_002700"
     CDS             complement(9739..10905)
                     /locus_tag="PC541_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2840151.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            11122..11973
                     /locus_tag="PC541_002701"
     CDS             11122..11973
                     /locus_tag="PC541_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840152.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
BASE COUNT         3675 a         2319 c         1284 g         4725 t
ORIGIN      
        1 tttcctaatt acaattgaaa tcattctaaa atgataatgt tattttatat tactatcttt
       61 tttgtacaac aaaaactaca aggattctat attctatatt tttccataaa aaatacccct
      121 tattatgcaa ataaattatt tgcataataa ggggtatggc taagtaagtg ttttaaaatt
      181 ttatgatata aattaatatt aataatagac ttaaattaat aatttatgtt tagatttatt
      241 ttttaggtaa gtgtaaacat tctccattta catctgccaa agcttccatt aaagcttctg
      301 aagtacatgg atgaccaaat atcaactcac ttgcttcatc agtaggtatc tctaatgctt
      361 taaatgatgc tgcatgattt ataagttctg ctacaccaca tccaaacata tgtataccta
      421 aaatttctcc atatttagca tctgctacta ccttcacata tccttgttct tgaccagaag
      481 ctaaagctct tccatttcca gcaaaattaa acttaccaac ttttacatta tatttttttc
      541 tagcatcctc ttctgtaata ccaactgatg ctacttctgg aattgtataa acacaactag
      601 gtaatgcacc taagtcaact tctttattca cacctagagc atttgaagct gctacctctc
      661 ccattttaaa agcagcatga gctaacatta cacctccagt aacatcacct actgcatata
      721 tgcttggtat acttgtttcc attttactat ttacaactat tgaaccttta tctattttaa
      781 tatctaagtc ctctatgcca gaaagattag cttctcttcc tatagcatat agacataaat
      841 ctgcttttat tggctcttca ccttcaatgc acactaatat gttatttcct tcttctttaa
      901 attcagaaac tttcttttta gttaatacat ttataccttt tttactaaga gaatatttta
      961 aagattcgct taactcttta tccattcttg ggattactct atcttccatt tctactatag
     1021 ttactttaga acctcttgaa ttgaatatct ctgcaaattc acaaccaatc actccaccac
     1081 caattattac taattcttct ggcactgttt ccaaatctaa tgcttctgta ctagttatta
     1141 ttaaatttga ttctattcct tttataggta atatccttac tttggaacct gtagcaatta
     1201 ttatattttc tgtatctaaa acttttccat ctgataaaat tactttatgc tcttctttaa
     1261 cactagcttt aagattaaac acatctacat ctctactttt taataatccg ccaacaccag
     1321 ctgtaagttt tttaactact ctatttttat attttatggc cttcttaatg tcctgttctt
     1381 tatctacagt tacttttaca cctctctttg ataattggtc aatttcttct aatatttctg
     1441 ctgtttttat ataagtcttt gtagggatac aacctctatt taagcaagta cctcctagta
     1501 tattttcttc aactaaagca actcttccac ctaaaagagc tgcttttaat gctgataaat
     1561 atcccccagg tcctcctcct attaccacta catcataatc atgattacac tcttttttgt
     1621 ttattttttc ttctttaaat tctatatttt gattctcttc aactatatct tcttttgaaa
     1681 catttccaga taaatattta gcttttactt cctcttgatt ttcccctttt tctgctatta
     1741 cacctattac tgtaaatact ggtaatactt caccttcctc tttatgtatg attacagcaa
     1801 gtgtaccttc tccttctgac tctatctcca tattaacttt atctgttgtt atttctagta
     1861 taggttctcc aatctttact tcttcgcctt cttgctttag ccaagatact atagtacctt
     1921 cttccatggc tactcctgct tttggcatta ttacttctac tgacataggc tctcctccta
     1981 tttattttct tatattttaa ttaagcaaat catattcaca tatagttact gttaaaaata
     2041 attcttaata tataaaagct ttaaatttac tgaaacaatt ataataatat aatctattca
     2101 aactatttat atcagtaaca aaatatcaaa acctatacaa gcattgataa aggattttct
     2161 aacaattctt ttaaatcttt taagaatttt gcagctactg ttccatctat tactctatgg
     2221 tctgatgtta atgataagtt cattataggt tttattttag cctctccatt tactggaaca
     2281 aacttatctt gtgtagcacc tacccctaag attgcacttg aaggcatatt tattataggt
     2341 gtaaatgtag ttataccata cattccaaca ttacttattg taaatgtatt accttcttgg
     2401 tctgctggca taagtttccc tgtctttact ttttccgcaa gttctttgct ttcttttgct
     2461 atttctttta aagacttttt gtttgcattt tttactaccg gaacatataa gccttcatct
     2521 aaaccaactg caatagctat atttacatct ttatatctaa atataccctc atctgtccat
     2581 gctgaattta aggcttgatg atttggaagt attttagata ctgccattac tattaaatct
     2641 gtcatagtta attttactcc agttgattct tttactgtgt cgattaactt tgctctaagt
     2701 accttcaatt ctgtagcatc tacttctata ttgaatgtaa atacaggagc actaaaataa
     2761 ctttctgaca ttcgttttgc tacagtagct ctcattggat ttggcttttc aaatattcct
     2821 tctactgtgt taaacatgct tgaattttca ttttcaattt tcagctcttt tttctcacta
     2881 gatttagatg tattagtcga tacaatagtt tcttttgcag ttaatttttc cacatcacac
     2941 tttcttatct ttccattata tccagtacct acaatagttt ctaaatctat atttaaatct
     3001 tctgctattc ttctagctaa aggagtagta tttgctttat tatcttttaa ataatttaac
     3061 acatctacta aatgaatcct tccattttct ccacttccaa tcagcctatc taattttata
     3121 ttgtcttttc ttgcctgact tctagctgct ggtgtggctt ttattttatt tgccaccata
     3181 tctatccctc cttatctaac aattaaagat tttattgctt cttctatttc atcaactctt
     3241 gggacaactg ctttttctaa ctctggatta tatggtattg gcacatcttt tccacaaatt
     3301 ctttttactg gagcatctaa ataatcaaat gattcacttt ctgttatcag tgctgatatt
     3361 tctccaccta gcccaccagt cttagctgct tcatggcaaa ttaaaactct cccagtttta
     3421 catacactct ttactatagt ctctttatct aatggatata aagttcttaa atctataatt
     3481 tcaacattta tattttcttt tgatagagtc tctgctgctt cttcaacact ttgtaacatt
     3541 cttccataag taattactgt aacatcagta ccctctcttt taatatctgc ctttccaatt
     3601 tcaattacat agtcatcttc tggcacaaaa cctttttttc tgtataacaa tttattctca
     3661 acaaatatga ctggattatt atctcttata gctgctttca acaatccctt agcatctgct
     3721 ggtgtagaag gtgcaactac ttttactcct ggtacatgac aaaaccaagc ttctaaactt
     3781 tgtgaatgtt gctctgctga accagttcca gaacctcctg ggcatctaac aaccattgga
     3841 acttgtgctt ttcctccaaa catatatctc atttttgccg cttgatttac aattgcatcc
     3901 atagaaattg ttacaaagtc cataaacatt acttccataa taggtcttaa gccagttgct
     3961 gctgcacctg ctgccgctcc tgctatagct gcttctgata taggagtatc ccttactctt
     4021 tctggtccaa attcatctat cataccaact gatacaccaa atgctcctcc atatattcct
     4081 atatcttctc ccataaatat tacattttca tctcttctca tttcttctga cattgcttcc
     4141 tttattgctt gtgcatatgt taattccctt gtactcattt ttattttcct cctttaccat
     4201 tcttagtctg catatacatc ctctaataaa gactctattt ttggatttgg gctattttga
     4261 gcaaactcta ctgcatcttc tattgattgc tttgcaaatt cttgaatctt atctaattca
     4321 tcttcattag atagattatt ttctataaga taatttttta aaaattcaat agggtctttt
     4381 gctttccatg attctatttc ttcttttgtt ctatatacat ttgcatctga ttttgaatga
     4441 cctaaccatc tataagttct actttcaatt aatacaggtc cttctcctct tctacacttc
     4501 tcagcagctt tttgtactgt ttcgtatact tctattgggt tatatccatc tattgatatt
     4561 ccttctattc cataagaagc tgctctagtg gcaatacttt ctatattcat atgacgtttt
     4621 atgcttgttg acataccgta taaattattc tcacagtaaa atattattgg caatttccat
     4681 attgaagata aatttattcc ttcatgaaaa gtaccttcat ttgatgctcc atcaccaaaa
     4741 ctacataaaa ctattttgcc tgttttttta tactgttgtg taagtgcagc tcctggagct
     4801 attgtaagac ctccacctac aactccattt gctcctaagt tacctgactc aatatctgct
     4861 atatgcattg agcctccttt tcccttacaa aatccagttt ctttacccat aagttcagcc
     4921 atcattttat ttaggtctat tcccattcct ataaattgac tatgtcctct atgagttaat
     4981 gatactaaat ctcctttttc taaagccatt acagccgcca cacttgacgc ttcttgacct
     5041 acagataagt gagttgttcc atgaaccata cctcttgcaa aaaaccaact aactttctct
     5101 tcaaattttc tagcctgatt cattctctta tacatttcaa gcaatgtttc ttttgatatt
     5161 gacatataca ttccctcctg ttaactttta tctatctttc tattagtcac aatcttctaa
     5221 taatttaagt gctaaactgt gattaattgc taaactattt ataaatttcg cttttaatga
     5281 tgcctttatt gcctctattt tttcatctcc tgaagcgaca cctattgttc tttttatctt
     5341 ttttaaatct tccagtttca ctcctaatac tctttgatta aattcaagta tagaagtttt
     5401 tccttcttta tcaaaggctt gtaaacaaat tgcacctatt gagttgtatt tttttaaatc
     5461 ttctatatct gcttcattca tgtatccaga agccattatt gttgagttta aactcatagg
     5521 atttcctata cccacaattg ctatagttgt tttatctatt aaatctagta cttctttaat
     5581 tttttcgtct ttgcaaagtt tctcttttgt agacaagtca gacataacag ctggagcatg
     5641 taataattta aaatcacctc caaaagccct cgctaaactt acggctattt gatttgcatg
     5701 aatatctatt tcattgtctc caatccctcc taatagaggt ataaatgtaa catttttaca
     5761 gttacttttt tccacatatc ttggtatttc ttttagtgtt gttcctattg aaacacctat
     5821 tatatcattt tcctttacta ctctagttaa atattcagat acagctcttg ctagttcttg
     5881 tttttgtgtt agtgcatcct gtttatcatc tactactatt acttctttta atttatattt
     5941 tctctccaaa cttctctcta ttttttggta atcatttttc aaaacatcaa ctatctgtat
     6001 ttttactatt ccttgttcaa aagcttcttt taatattctt gatattgttg gtcttgatat
     6061 tcccaattga tttgctattt cctgttgatt caaattatct tcgtaataaa gtgaacaaca
     6121 cttcatcatt aatcttaagt ctccaatttt tttcatttgc tttcctccaa aatcaagctt
     6181 ttcaattatc tttacatttg ttcaatcatt ttacttaatt tcagtaacat tatagaaggg
     6241 gttcatataa aaagtcaact gtttttttgt ataacaaaaa aaaggtgata cttttcttgt
     6301 accaccacct tcataaatag ttgaaaaatt gcttcttagt gcatattata tattacttct
     6361 aattgttaaa tatcatctcc ataaattaaa aattaattgt ccacttataa tgctttaaat
     6421 aacaataact tattaaatca cttctaaaaa accaaccatt tcatcattta aagtcttctt
     6481 atatttttat tattcgcttc tctaaaaatt cttcaataat cataaatata taatagtttt
     6541 tatagggaac atttacaata gaccttaatt aaacttaaga ctttataaga aactttttca
     6601 ttatttagta tttaattcat taagtatagt actatattct ttatcaagct tataaaaaga
     6661 cattatgata atttgtaata ttgttataaa tataggtagg tatataaaac atacttttat
     6721 tgatactata gctaaatcac tttgagtagc tgatgttcct acataaccac caattgataa
     6781 tactgcacct aatattgctg aacctaatcc atttcccact ttaaacccaa aactagatgc
     6841 actatttata agcccctcag ttctatatcc agtcttccat tctccatatt caatagtatc
     6901 tgaaacaata gcaaacattg ttgatgctat tccagcactt cctattccct ttatagcact
     6961 tgcaaatatt actcctatat gattttgtcc aactattata aacataacat ttgacgatat
     7021 taatattatt gaacctacta ttaatacatt tctttttcca aattttttta taaaaggagc
     7081 aaccataaac atagctataa tttgggatat atttgaaacc atatttatag gaccaaataa
     7141 tttttcatct cccaaaataa actttgcata atatacggat gaaccaccat ttatcgcaaa
     7201 tacaataaat atgcaaacta aacataatgt tatttgtatc cagtacttat ttctaaataa
     7261 agatttaata cctattttaa atggtattac ttcattccta tcttgcttaa ctgcttttac
     7321 tctttccttg gttccagtaa atgttataaa aaataccatt atagatgcta ttccaaatac
     7381 aaagaatgtt ttagtccatg ctgataaatt atttccaaag tattcaacta gaggtagtgt
     7441 aagtccattt attagtaagg taccacaagt ggctaatatc attctaaata tacttaatac
     7501 tcctctttca tactggtttt gagttataag tgaatttaga gttgcatagg gtacattgat
     7561 tgctgtataa ataacagtag aaactaagtt atatgtaata aaaatataaa ttaatttaga
     7621 agtgatacca agatttgatg gaacagaaaa taataatact gctgctatag caaaaggaat
     7681 cgccattctc aaaatccaag gcctagcctt accgtatttg gatttagttc tatccactat
     7741 aattcccatt gttaagtcac taaacccatc cagtatccta gatacaagca taatactacc
     7801 tacagctgct gcactaacat ctgcataatt agtataataa aacagcaaaa atgctgacat
     7861 agctgagtat atgatattgc accccaaatc cccacaacca taagaaattc tttcaaaaaa
     7921 tgaaatcttt tcatatctaa tcgtagttgc tttaacatta acttctgtat tcataatacc
     7981 atactccttt ttgtaagatt tgccttctaa tgtttcttat ttcagagcat aagatttacc
     8041 tttgctctga aaattttagt aatgatttta ttaatcgaca tcttttgatt catctactat
     8101 atattcaaaa aaatcaaaat cagcatagtg tttatgcttt acacgatctg cacaagttaa
     8161 tcctataaac gtacctgtaa attctccata tttacagtac tcatctgaaa acttagttgt
     8221 atcaaatacc tttcctattc tctggtagtt tttttcatca taactccatt caaaatatga
     8281 cttacgacct tgtacataca gacgaagata tattggtata tcttttattg gtatcctagt
     8341 atttaaaaat tccgttttct ctccattttc aagatgtatt attgatagtg cactttgacc
     8401 caatgtttca ctataatact ttcttaaatt tatataattc atattatcat aatacattat
     8461 caaacccgca ctatgctgat gtacctctgg gtaaaactcc atcttagtgg ttattcttgc
     8521 atatactgaa gttaattttc ttgccaatat gctcacttta tttaatgatg ttcttgattc
     8581 ttgtcctcta atccttacat atcctggtct agacttaaca tctgcaaatg ataatggtga
     8641 aatcctagga gcatagtact gtaaacctaa ttcatttgaa tcaaagtcat caaaacttgg
     8701 tatagatgaa actaaatgtt cttctagttt actttctgat acgtattctt ttgccaaatt
     8761 actttcatct tccattcgaa gccaattatc ctttgtccat ttcatttttt gaatagctgt
     8821 ttctctacct aaagtacacc ttaactctgg tacaaatggt ctagatgtta gatgaactaa
     8881 atatacttca cctgatgttg tttctatata gcttccatga ccagattttt gaagttttga
     8941 ttctggatta tagtatttag gctttagatg gtcagggtca tgtctttcat aaaaatcgcc
     9001 tggaattgaa gttacaattg gattcattga atctttttca taaggtcccc atatattttt
     9061 agcccttccc atagttacac tatgaccata tccagttcca ccttctgcac acattatata
     9121 atagtaatct ccacgctttg ttatatgtgg tgcttcaata caacctctat cagttccccc
     9181 tgaccatatt ctctttggat atcctactat ttctttcttt tttgtacagt attccactaa
     9241 acatataaca ccaggttttt catatccttc tcttgtctcc cagtctaatg atgcaatcca
     9301 ctttttacca tcatcatcat gaaaaataga cgcatcaaaa cctgatgagt gtagatatac
     9361 tggttcactc cactctccct ttatatcttt tgatgtaatt aaataattat caacatcaaa
     9421 atatcttgca ttcatagagt tcataattcc ataaactata taaaataaat cttcttcttc
     9481 acaataagtc aaacagggtg cccaaattcc tttagatgaa ggaagcttct tcaaatctat
     9541 ttttgtttca tctgtcaaaa tatgagtata taactcccaa ttttttaagt ctttagagtg
     9601 ataaacaggt atacctggaa accattcaaa agatgaaact acaagataat aatcatctcc
     9661 ctttctacaa atacaaggat ctggattaaa acctggaagt atagggtttt taatcatatc
     9721 ttttcctcca attttagttt aatattctat aggataccat tcttgagcta cacatttgac
     9781 taggtcccaa ctatcccatc ctatccaacc ttcttcatta actgtatcta gtaataactt
     9841 ataattccaa tagtagtgac cacttccatt tctccatgca tcaagctgtg cttttgctat
     9901 cttcctatat aattctcttt tatcgtcttt tgataattta tctatatttg attcaatccc
     9961 atttagaggt gattgacctc catttgtatc tataccacaa gcatatgaat taaataagct
    10021 ccactcccca catataacag gaaaatattt ttgcatttgt aaaatatctt tagcataatt
    10081 ttccctaata tatttgagat agctatcaat actttgttca catccatctg cttcagctaa
    10141 catcaaatat tggtgtgtat ctagaacaac atttttaaat tcttcctctc tcataaagtc
    10201 tttccatgct tttaattcaa atgcatcatg aaatactata acgttttcct ccctcatata
    10261 aggacgcatt accttatatg cttttatata aaaatcacgt aaaaactcta atgaattagg
    10321 tttactcccc cttgccatct ccttatctac agccttaaac cgatttggaa cattcattat
    10381 gttccacatt ctttctgtta taggctcatt taaaatttgg attccataca attcatgcct
    10441 catcccataa cgttttgcaa gtctttctaa tacatttaat gtaaacgcta catattctgg
    10501 attttgtgac cagctacata tacctgatat tcctccatta tcaaatccat tttggcttcc
    10561 tggaactgta tgtaagtcta tcaatatact aagcccatac ttatctgccc aagcaaatgc
    10621 tttatcaagt tctttaacac atcctatgaa tggttcacaa tctccaaaaa taaagtatgg
    10681 tactggtata cgaactgaat taaatcccat agatttaatc attgcaaaat ctctctctgt
    10741 gatgtattca ctacgatgag tcttaattct agattcatat gcctctcttg aaagctgtct
    10801 tggtaaatag tattcatctt cagcttctgt gccttcaaat aaacttgagg tcatccattt
    10861 ttctaaaact aaccaacctc ctaaatttac accttttata tacacataaa atcctcccct
    10921 cttatcttat ttaaataggt atattttgca cttataataa aataattttt ttaagttatt
    10981 ttaatataca gttattatat ttgattttat ccgttttctt aagtatgttt attatctaat
    11041 ttattaatct atattatctt tttaatattg aacattataa atttactatt tataatttaa
    11101 aattataagg aggatattat tatgaacaga aataattttt cacatgaaat aattgaacct
    11161 attgataatt tagatgttaa tttcaaacta tttgatgata gtggtagtta tgtagcaaat
    11221 cattggcata actcattaga aataatttat ataacttctg gtgatttaca aattaacatg
    11281 gagggttaca cttataattt aaaagctaat gagtgtatgt tcattaattc tggaattatt
    11341 cactcaacta gatgtattta tcaaaatacc tctatattat tgcaagtacc attgtctttt
    11401 ctaaacaaat atatccctga ttttaaaaat tgctattttg actttaaggt taatgccaat
    11461 gacaataact ataaaaaaaa ttcaagtaaa gttaaactta tattagaaaa catgagagaa
    11521 attaaactta gttctcctcc tgcagctaat ctgctcttta caagcttatt atttgaactt
    11581 ttatttgaat tatatactaa ctttaaaata tctgtaggta ataaaaatat gaaaaaaaca
    11641 gtacttgatt tgtcaaaatt tgaaccagtt ttagaatata caaagattaa ttacaaaatg
    11701 cctatttcta ttaacaaaat cgctaaggta gcacacttac aaccagaata tttttgtaga
    11761 aaatttaaac agtatatggg acaaacttac ttagagtatt taaatgatgt gcgtatttct
    11821 catatttata aagacttaat taatacaaat gataccttat gcagtatatt agaagctcat
    11881 ggatttacta atacaaaatt attttacaga atttttaagg aaaaatttaa atgcactcct
    11941 aaacatataa gaaaaaatct acctaaaata tgaagttata tgttatttat ctttataata
    12001 aaa
//
LOCUS       DAMJZE010000120        11860 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJZE010000120 DAMJZE010000000
VERSION     DAMJZE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.122"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            112..810
                     /locus_tag="PC541_002702"
     CDS             112..810
                     /locus_tag="PC541_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840153.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            988..1878
                     /locus_tag="PC541_002703"
     CDS             988..1878
                     /locus_tag="PC541_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840154.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2271..3227)
                     /locus_tag="PC541_002704"
     CDS             complement(2271..3227)
                     /locus_tag="PC541_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2840155.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3930..4925
                     /locus_tag="PC541_002705"
     CDS             3930..4925
                     /locus_tag="PC541_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2840156.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4903..5766
                     /locus_tag="PC541_002706"
     CDS             4903..5766
                     /locus_tag="PC541_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2840157.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5763..6557
                     /locus_tag="PC541_002707"
     CDS             5763..6557
                     /locus_tag="PC541_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2840158.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6842..8221
                     /locus_tag="PC541_002708"
     CDS             6842..8221
                     /locus_tag="PC541_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2840159.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8669..9382
                     /locus_tag="PC541_002709"
     CDS             8669..9382
                     /locus_tag="PC541_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840160.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9654..11192)
                     /locus_tag="PC541_002710"
     CDS             complement(9654..11192)
                     /locus_tag="PC541_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2840161.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4326 a         1348 c         1804 g         4382 t
ORIGIN      
        1 aacctataaa actattaact aatatttgta aatgtatact tttgatgata aaatataatt
       61 aagttaagtt ggtttttata aacataaatt tattttaagg aggtgaaata aatgaaaata
      121 tctagtcaat atagaagtca atattcattt agatatgaaa gtaatataaa taatacaaga
      181 ataaatgaaa gtatggttaa gaaaaatgaa actgtaggaa aagacactta tttatctaat
      241 attatgaaac aaaagcaaga acttaatgat agaattagag atttaaaata tagacaagag
      301 gtttatacta aaaaaataaa tgacgcaatt aagaacttat gtaaatcaga aataagagaa
      361 acaactaata atttttctaa tatagaaata ggtattaaaa atagcattat agaagagaaa
      421 aataaaagta caatgttaga tgaaaattca acttatctaa atacaaatga tgaaaaagaa
      481 tctttaatca ctaaagagtc taatgaaaaa attgaagaag aaatattaaa tgatgaaaaa
      541 ttagaagagt tagaacaaaa aaaggattat aaagaggatt ctaataaaaa agagaaagta
      601 tctgaggact tatctttagt aggtaaaact cgtgaagagc ttgaaaatat gcttaaaaat
      661 tttataaatt taacacaaga agaaataatg aaacttgagt cgagaataga aaagttagat
      721 aaaaatgctg aagaatacaa acaaaattca aagactaata tatttgataa aacagatgaa
      781 caaaaaaaac atataaatgt actgatttaa acaatatgtt ttaaaaaaat aagtttatta
      841 tataatgaaa atataaaggt acaatttcaa tacacttcta ttaaaccaat aatttatgaa
      901 ccagacttaa tttcaattta ctaataacca tatatttatg agttaatagt tttatgtttt
      961 aaaaagaatt aaataagggg gagtagtatg aagaagaata tattaactac tatgattacc
     1021 atgattatta tattgatgat gagtatgaca gtaatggcag agccatatca agaagatgaa
     1081 acatctagct tgaaaaaaca gagtgcaaat gaacgtaatc agactaatat aggcacaatg
     1141 gatggcagag tgcaaacaaa tgcaataagt aaaggtgcta attatgaccc taaatatcct
     1201 cttaaaggaa tgttaacaca acttggtctt aatacaacag gtaaagaggg atgggattta
     1261 ctctatggag gaaatcccaa tggagttgag cttcgagcag atggtaaata ttattatata
     1321 aataagggta tggaagggct aggattttat gcggcagcat tgtcaggtgg ctcatatcag
     1381 agaataggct ttggaaaact tgaccctata gaagtaatag ctgatggaaa ttggatttct
     1441 tacaaacaag ctcaagatac acttacagta atcaggaatt ttttaaatag ctttgactgg
     1501 agaaatgcat ctgagatgga aagagctaat agagctgcaa aattggtaac cgaagcaaaa
     1561 tatgcagact ctaaatattg taacatagtc tatggtaatc tggttgataa acgtggtgta
     1621 tgtggaagtt ttgcaagtag ctttcattta ttaacaaggt taatgggaat ggattcacta
     1681 tctatattaa atccatcatt aaatcatgct tggaactata tacaaattga tgggaaatgg
     1741 tataggtttg atgggtctga aatatcagct tttggtggat cattagactt tgattaccgt
     1801 aaattaaagg atgcaacaag agagatgact acttactatg atgctaaggc attgtctata
     1861 ttaggattta atcaataaaa atagggggag gcttcttata gagccttttt ttattgattt
     1921 gaaatttaat aaggaatttt atatgaaaat ttaatcaata aaagcaaaaa aggctcttta
     1981 taaagccttt ctaagtgttt ttttaagtaa aattttttat atcaacatca gttttaatca
     2041 aaacatataa cattttggac ttacttttaa aaataaaatt attaaaatat aatattaatc
     2101 atgtttttag tttattatat tctataatag tggtcatgtt gactattgta gttttattaa
     2161 gaatagttgg tttatataaa tatatgcaaa gatagcttgt tgtttacttt acttattagt
     2221 atataggaca agatactcta ataaatttgc ctattttaat atcttttgta ctaaaaccaa
     2281 ctaaagtttc ctttattatt tcgttttgaa taatacaaag cataatccga ttgtttgtat
     2341 aaagactgga aagtatcaga gttattttct aatttagata tacctataga acaagaatta
     2401 ctctctaggc ataattctaa aaccttcttt gccttatttt caacattctc ttttgattga
     2461 ttactatatt caaaaacagc aaattcatct ccaccaagtc ttccaataat agcatcattt
     2521 acgaatactt tagtcaaaat atttgaaaaa tgtattagta tttcatctcc tcgaacatga
     2581 ccaaagatat catttacctg tttaaaatta tctaaatcaa agatgtaaaa gtatccatgt
     2641 gaaacagttt taagcatttc ttttatgtgt acttcaaaaa aatgtctatt gtatagtcca
     2701 gtcataaggt ctttttctga ttttagttta aattcatttg ccattgctag tgcttcttgt
     2761 gctttttcag ttattgtttt agggtaatac tcatctttca tttggtcaaa gtttggaata
     2821 gaatggtgaa catggataag tttaatcata tcatctgtaa ctctgtatac catagtaaat
     2881 cgagtatcca tttctacata gatatctttt tctaggtaat ctttttctat tacccaaaga
     2941 gtaccatata ctataaaagc ttcgtctgtg actttggtac atgaatacca ctcatctata
     3001 atctcaaagt ctatttgctc tgattctgtt tggtcttttt caagacctat aataaattct
     3061 tctaatgttt cgtaaaattc atgttttcct gttccaatta atgcaaggtt gtcatctaat
     3121 aaggaaataa taaatttaag gttttcttct tctggattta aaatataaat atgccaaatt
     3181 ttttttgata aatctcttaa ttgtttttgt attgtattat tttccataga ttaccccctt
     3241 aagatttaca gtttatattt taatgtatta taagttgaac tcttattaca atattattat
     3301 aatatagaac taaagaaatt acatcctgta taggtaaaag tatatctttt acaataaaaa
     3361 tattacagta aaatgaccag ttttaatata cgtattatat ttttaattta taaacgaata
     3421 ttataaatga aatttaaatt caaccttgaa gaaataagct aaatgtatta gtatattaat
     3481 ccatgcttga acataaataa cctttatgat atgatatcaa taataatatt atgagaggag
     3541 tgccttgatg tcggagttat ataatggtaa ccaacaatta aatagttgga gacacatata
     3601 ttctgtataa tcagaaattt caagtgtatt tttaatacat ttaatttttg tgtcttattc
     3661 tgcctttata gataactgac atatgacaac aaatagtatt taaaatatat attactttgt
     3721 taaattatgg tgtagtaggc tttaatctgc tacactttta ttaattttgt atagtattat
     3781 tttgataact attttgaaat agagagcatt aatttttcta gtccacagga gttttctgtt
     3841 tttgtgctgt taaatataag cacagaaaaa ggcagatata tacctgtgga ctttttttat
     3901 ataaattatg aaatatggag gtagttaaaa tgagtataat tgaacttaat gaactgacta
     3961 aagcttatag aagatttgag aaaaaggcag gatttatggg gagtgtaaag tctttattta
     4021 ataggaaata tacagaaaaa acagctatat tgtcatttga tttaaagata gaagaaggag
     4081 aatttgttgg attaataggt caaaatggag ctggtaaaac tactcttatt aagatgttaa
     4141 ctggtattat tgcaccgtct tctggagaaa tatctgtaat gggttattac cctaacaaat
     4201 tagaaaataa ttttaaaaaa caatatgctg ttgttatggg ccaaaaaagt caactattct
     4261 ttgaattgac accagcagat acgtttcttt tatttaaaga attatatgat atatctaatg
     4321 aagaatataa aaaaaacttg gattacttta tcgaattatt tgaggtatct gactatttaa
     4381 atgttcaagt taggacgctt tctttaggag aaagaatgaa aatggaacta atagtagccc
     4441 ttctacataa tccaaaaata ttatttttgg atgaaccaac tataggctta gatgctatag
     4501 cccaaaaaca aattaggtat ttcctaaaag aagtaaatga aacaaaagga actactatta
     4561 tacttacttc tcattacatg gaagatatta agagtctctg tagtagaagt attgttgtag
     4621 aaaatagccg taaaatatat gatggttcta cagagaaact atttaatcat tttcaaaaat
     4681 ataaaaaaat aacagttagt ttttccaatg aaactaacat tgtatttcca aaagattgta
     4741 aagttgttga acaaacaaat cataagatat cagtaattgt gccaaaagaa agaggaaatg
     4801 aaacgattaa atggattttt gataaatatg atgttaaaga tgtaatgatt gaagaagaag
     4861 atataggtag tattgtagag agaatatata agaaaggaag tgatgagtat tatgagcaac
     4921 tataatgaag caagttcaaa tgattcaatg ttttataaat ttaaacagag tattagaagg
     4981 tattatgaag tagccaaaat taactttaaa gtgcaactta tctggagatt tgatgtggtg
     5041 gttaatatgg tgtttacgat tgtaaaaatt ttatttgctt atattttatg gaaagcaatt
     5101 tttggtgaaa ataaaatagt ctctggtttt acatttaact ctatgttgtc ttactatata
     5161 ataagctcat ttttagcagg tattgatatg tcaaagagta ccagtgaaga gatatgtaat
     5221 cgtgtaaaat tgggcacctt ttcaaagtac atgattattc caattaatgt tcaaaattat
     5281 tttattgcta aaaattttgg aatgacatgt ttttattttg tatttaatat gatttctgca
     5341 tttgtatgga tttttatatt taaaatagat tttgccttta catcaaatcc agttcaaata
     5401 ttaatttcaa ttttgatgat ttttctaggg tttattttta tgatgaaact ccacttcttt
     5461 attgggattt tgtcatttaa atttcttgag attagttttt ttaaaatgat aattgaaaat
     5521 attgttgaat ttattacagg aacaatgata ccacttgtac tcttaccaga aactatagta
     5581 acagttatga aggtatttcc attttattat acaacatatt tgccatctat gttactaata
     5641 ggtagaaaca gcaatgaggc aatacaagga ttaattattc tagcattatg ggttttattt
     5701 ttttctgtag taaataaagt tatgtataaa agaatgagaa cattatttga tggggtgggt
     5761 atatgatgaa aaaaaatttg aaaataatat ttatgttatt aaaaatgaaa ttatcaaaga
     5821 tgatggtatt tagattcgat ttttttggag cattttttgt agatagttca ttatttatat
     5881 tacaacttct aatgtttaat agtatatatt ctcatgtaga ctcaataggg ggatggcaac
     5941 aaggagagat gttaatattt ataggaactt tctcattaat aaatgcgata aatatgacga
     6001 ttttcttttt tggtatttat gatattccac gaaaaatcca agaaggagaa ttagactact
     6061 atattacaaa accattagac ccactattta ggttaacatt tgaaaatatt aatattggtt
     6121 catcaccatt aattctagca agtatactta ttgttttata tggaatatct gagttaaact
     6181 cagagataac tttagttatg ataataatat atattttcat ggtgttattg atgacattac
     6241 ttttttatga tgtatgtatc atacttagaa cagtttcatt ttttgttata tcatcttctg
     6301 cgataatgag attagaagat aatttattgc caatgaatat gaaaattcct ggtgtaatat
     6361 atgaaggtgt ttttaaagta ttattctatt taatcttacc atatggaatt atgtcgacaa
     6421 ttccgactca aattttggct gggacaataa caattaatgg attaatatac tcaattttaa
     6481 ttgtattttt gtttacattg tttatgtgga gattttggaa gttaggtatg agacattata
     6541 aaagtgcaag tagttaataa attagtaaaa atagacaatg tataactata tataaatacg
     6601 tttcatatga cactaaacta tgtgaacaag tatttgcgct aaaggcactg aaagctataa
     6661 aattattaaa gtaaggattt aaaagtttct ttagtagaaa tataattgtg taaaaaagca
     6721 agattaatta aaactatatc caaataatta gatataattc ttatgcagaa aggaggtctg
     6781 taaatgtcta attgtttgga atatatttta aaaataatta atgtacagaa aaataaacaa
     6841 aatgagtgat gtatattatt cagaaaatca tgaacaagat aatctttatg agcaagataa
     6901 tttactggca caaagattta atttatttac tctattgaag tttgcatttc ctactatttt
     6961 tatgatgatt tttatggggt tgtatacaat cgtagatact atatttgtat caagatttat
     7021 taatacaaat gctctatcag caattaatat tgtatgtcca attattaatc ttatagttgg
     7081 aattggaact atgcttgcca ctggaggaag tgctattgta gcacgtaaga tgggaaatgg
     7141 tgatattgag ggagcaaata aagctttttc tttaatcgta ttatcaggag ttgtaattgg
     7201 ggtaataatt actggtatag gtattgtatt tatggattat atcattatat ggttaggagc
     7261 aagtgaagtt ttaattaaat attgtcgttc atatcttttt attatcttgg tttttgcttc
     7321 tgcaaatata ctacaagtat tatttcaatg tttatttgta acagctggaa agcctcaatt
     7381 tggattatgg ctagtgttag gtgctgggtg tgctaatata attcttgact atgtgttcat
     7441 tgttctgtta caaatgggta ttagtggagc agcacttgca actggagtag gatacctaat
     7501 tcctaccgtt tcaggtatgg ttttcttttc aaaaaataaa gggttactta aattttgtat
     7561 acctagacta gataaaagaa tattatttga agcttgtttt aatggttcat ctgaaatggt
     7621 gggtcaacta tcaacagcag ttactacttt tttatttaat gctatgatga tgaaattaat
     7681 tggtgaagat ggggttgcag ctattacaat aattatttat tctcaatttc ttttaacttc
     7741 gctttatata ggtttctcaa tgggtgttgc accaataata agttataact atggaagtga
     7801 aaataatacg cagttaaatc atgtttttaa gatttgtata ttatttattt cagtagcatc
     7861 tttattggta tttatctttt ctatatcatg ttcaccaatc attgtagaga ttttttctca
     7921 aagaggaagc cgagtttatg aaattgcaag gtctggattt tatatatttt cttttagttt
     7981 tattttttgt gggtataata tttttacttc gtctatgttt acagcattat caaatggaaa
     8041 ggtgtctgca atattatctt ttctacgtac atttggattt ataactatag gtttattgat
     8101 attgccaaat atattaaata taaatgggat atggttagca gtaccaagtg ctgagatttt
     8161 aactttattt ttaaccattt tttgtatatg taagtataag aatagatata aatatatgta
     8221 gatattaaat aaatagttta ggtagatatt ttatgtttta ataagtaatt caattagtgg
     8281 gagatatata ctattgaaaa ttaaagttat aattgatata aagaattaat aaaaataaca
     8341 tataaaagtt taaaagggag ttgaacttaa agtttgatta gaaaaagtca attttaagac
     8401 aactcctttt attttactat tgtattaatc tgtatctgta agtgtattag tctaatatta
     8461 taaactttgg atgaaattaa ttttctatag gttttaatgt atcatacact ttattttaaa
     8521 gaaacttatc ttaattttca tagtgattga atttgtctat tagagatgtt actattagta
     8581 atgtaaaagc aagagtagtg aatataaaaa acaagatagt gatactgcaa gattagaata
     8641 taataaattt ttatggagag atttcaatat ggatacatgt tttaataaaa aaatattact
     8701 tgttgatgat gaaaaggata tagtagattt aattgaagaa gtactcataa atgatggttt
     8761 taaaaacatt attaaagctt ataatggtct agatgctatt tcgctgtgta agatagcatg
     8821 tccagatgtt gtcattcttg atataatgtt accagatata gatggtatag aagtgtgtaa
     8881 aaaaattcga gagttttcat actgttctat tcttttttta tcatcaaaaa atgatgatat
     8941 tgacaaaata ctaggtctta gtagtggggg agatgactat ataacaaagc cattcagtcc
     9001 aagagaaatt gcttttcgag ttaaagcgca attacgtaga caacagtatc aaagcattgt
     9061 accatcagat agtgaagtta ttaaaatagg agatattact attgatatag aaggaaaccg
     9121 tgtatataaa gatagaaatg agattgagtt aactggaagg gagtatcatc ttttaagcta
     9181 catggctcaa aatgtaaata aaattatagg aaaagaaaga ctctatgaac aagtatgggg
     9241 agtatatagt agtatttgtg acaatacaat tatggtgcat atacgacata taagagaaaa
     9301 aattgaagat aacccctcaa atcctaaaat acttataaca gttaaaggac taggatataa
     9361 acttgtgaat agaatcgatt agaatagatt ttaatattaa tgaagatatc tcaagttgaa
     9421 tttttagtta atgaggcatt cttttatggg taaaatcaaa tatattttcg ttatctcaac
     9481 aatgaagaag aggtctatct ctattttgag atagcctcat taagtttata tcctggtaat
     9541 gtactttatc ttctaataaa atttttttag tttatttgca gtgaaacatt aactagaaca
     9601 ttaaaatttg cattgaaata ctaattaaat aatattgtat attgtgtata aacttatact
     9661 aattgttttt ctatattaaa tactttatca atccaacctt tataaaattt ttcttcatgg
     9721 gaaactagga ttatacttcc tctaaactgt ttcaaagcat tttgtaaagc atcctttgtc
     9781 tctgcatcaa agtgattggt tggttcatcc aggataagaa aattacaagg agataacatc
     9841 atacaacata atttaacctt agattgttca ccaccactta aagtagatac actccttgaa
     9901 acatgctctt ccttcacacc acatctggct agatgacgtc taatctcttt tgtatttaat
     9961 tttggatatt tatctgctac tatttgaagt ggggttttat caggattttc ccattttaaa
    10021 tcttgctcat aataaccaat tttaacttgt tctgaaaact ggaaatctcc agatatacga
    10081 ggaatatctt tgactaatgt ctttaacaag gtagatttac cgattccatt aaaaccagtt
    10141 ataactattt tttgacctcc atcaacagag aaatttaatt ttggaagtaa agaatagtag
    10201 tatccaactt caagatttgt tatagtaaga gcattttgag ccgatatttc aatttctgaa
    10261 aattgaatat ttggttttcc tgtaaaacta ggaggagcta ttctttctat tttatccaat
    10321 tgtttttgtc ttcctcttgc aatttttgaa tttactccag ctttattttt acgtataaat
    10381 gtttcttctt tttctatttt cttttgttgt gcttgatacc ttctaatata atcttctctt
    10441 aaatgtgatt tttgtttaag gaactctgaa tattttccat gatatttctt aatcatgcca
    10501 aattctatat caagaatacc tgtagaaatt ttttctaaaa agtcaaaatc gtgggaaact
    10561 acaataaatg ccccattaaa tgtatttaaa taatttgaga gccattcaac atgttcttta
    10621 tctaaaaagt ttgttggttc atctaataat agaacatcat ggttagaaag tagtaatttt
    10681 gctaaaatta cctttgccct ttgaccacca ctaagctctc caacaacacg attcattcct
    10741 attgaatcta atccaagacc atttgctatt ttatttattt cattatctat agaatagaaa
    10801 ttacttgcta ttagttgttc ctgataatct gatgctttga ttagatattg ctcattttca
    10861 gatatagcac ttttttgata gagtaaattc atttcctttt ctattttata aagttcttca
    10921 aatgcagtgt gtaaatagag tgatattgtt gtatctctat tgatttctgc atattggtct
    10981 aaatggccta tattgatatt aggttgccac ttgatttctc ctgaatctgg aacaacttct
    11041 cccaataaaa ttttaattaa tgtactttta cctgtaccat tttggccaac tacacctata
    11101 tgttcacctt tgaataaatc aaagttagct ttctcataca gtgatttatc cataaaacat
    11161 tgtgataaat tagttacttt taataaactc ataattcaaa ctccttttct tttattaaaa
    11221 aatttatgga aataaaaaaa gcagaggaat ggcattgtaa aatgccgacc tctgcttttg
    11281 gaaaaacaaa aggtcataga caagcattgt tgtctatgac cagaaaatct atattatcta
    11341 tcggcaaata ggtaatttta aataagcata gccaaaaaac tatactaaac ctatatttgc
    11401 cgatgaagat agaaagcttt aagctttcag attaacctct acacaacgct tcatcgtata
    11461 catttgtgta gagttaagtt ttctaacaag tttaactaat tctatataaa tcataattat
    11521 tcacatccta atcttagtat taattctagt ttatctgtat ttaaattgta cttttgtact
    11581 ttgtggatta tcttatttat ttaaaatatg ttttattaat tatagcttaa gttatgttat
    11641 cataatactt agttatagtc aataagaaag ttatatttac tattattata ttaatttagt
    11701 aaatctatca ttacaaagtt atttgaagac tcgctatcaa taaaatttta tagtctataa
    11761 gaaaattaag attatgagtt ttataatttt gatataggga tactattttc ataaatatta
    11821 caacatgtag aagtatatgt ctattattgt tgaattttca
//
LOCUS       DAMJZE010000121        11777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJZE010000121 DAMJZE010000000
VERSION     DAMJZE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.123"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            326..1504
                     /locus_tag="PC541_002711"
     CDS             326..1504
                     /locus_tag="PC541_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2840162.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1854..2735
                     /locus_tag="PC541_002712"
     CDS             1854..2735
                     /locus_tag="PC541_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2840163.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2720..3093
                     /locus_tag="PC541_002713"
                     /pseudo
     CDS             2720..3093
                     /locus_tag="PC541_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3353..5920)
                     /locus_tag="PC541_002714"
     CDS             complement(3353..5920)
                     /locus_tag="PC541_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2840164.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5923..6528)
                     /locus_tag="PC541_002715"
     CDS             complement(5923..6528)
                     /locus_tag="PC541_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2840165.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(6902..8362)
                     /locus_tag="PC541_002716"
     CDS             complement(6902..8362)
                     /locus_tag="PC541_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2840166.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8732..9310
                     /locus_tag="PC541_002717"
     CDS             8732..9310
                     /locus_tag="PC541_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2840167.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9601..10851
                     /locus_tag="PC541_002718"
     CDS             9601..10851
                     /locus_tag="PC541_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2840168.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            11012..11581
                     /locus_tag="PC541_002719"
     CDS             11012..11581
                     /locus_tag="PC541_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2840169.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         4084 a         1942 c         1639 g         4112 t
ORIGIN      
        1 tacacataat tatcatacat gctatatgta attactatac ttatacacat aactatcata
       61 catgctatat gtaattacta tacttataca cataactatt atactttcca tacataatta
      121 ctatatttgg acacatagtt atcatacata ctgtatatta agatataata ctttttaaat
      181 tttatataac acagacacat gcaataaaaa agaaggtatc cataataagc tctttgcatg
      241 tacctaaata aaaactgtaa aatagaatat ataaaatcta atctatttta agtaaataca
      301 atttcacttt aaaagaggat gtactatgaa ataccatata tttttaatca taactactat
      361 attaataatc gaactatgta acatttacat atgcaaaata gcatcttttg gaaacaaaaa
      421 tataactata cataagaatt acaaatatat ttttttaata aaaccattaa ccttcttgat
      481 aataatattc acaaaattac cattaccact atattatata ttgttttaca tagtaagtca
      541 taaattgctc ttaagcagaa aaagttctac taacaaccat tttattccca atatggcagt
      601 aataagtttt tgtattttac accttattac tgtaagtaca atatcacttg ttaaaaatac
      661 aagtccagat ataatatttg caaatatgac ctataagtta attagtattt taatagcttc
      721 tattttagat tttatcttat ggtactctat gaataaaata aaagagcata tttatataat
      781 aactcaaaat caaatagagt tcagtctatt tttaaaattt atctggttca ctttaggata
      841 tatattattt gatagtatac aaattacatt tgctccacca agttctctaa cttcctactt
      901 tatattggga agtaacatat tattaatctt acaactaatt atattcatca atcatatata
      961 ttccattgaa aaaaatattt atttagaaaa agaacataat agactaaaaa tgataaaaca
     1021 agaacaatta ctaaaagtaa atttattaaa gaaaaaagct aacattgatg aattaacatc
     1081 agcatataat cgaaaatttg cttttgaaaa aatacagcat tacattgata acaatttatt
     1141 attttcattg atatatatcg atttggataa attaaagacg ataaatgata catatggaca
     1201 tttagctggg gataactata ttattaactt ctcaagtata gttattgaaa acttaggaat
     1261 agatgacata tttgctagaa taggaggaga tgagtttata gttattatgc ctaaatctaa
     1321 tgaaaacacc acaaaagaat ttatagagaa aataagtaaa attatggata atacgatatc
     1381 ttttagctat ggtattgtag aagtttctag cgaaaacaaa atgactgtag aagaaattat
     1441 taatattgct gatattagaa tgtacactaa taaacaaaat aatcataaaa ggaacaataa
     1501 ctaaaatgaa tatttacatg agcttacttg tagtctcact atgtttttta tcttattatc
     1561 tctatcatta taattttttt aaatatctgg acataatgat taataactct aaaaataaat
     1621 acacagtata tattatatat tctatattta attatttaat ttttattatt tgtacttatt
     1681 taaagtttca tctaattata aattggacaa tttttatgtt gattttattc atacaaatta
     1741 aagtaactta taaaatctcc acaataaaaa acatttttta ttcactaaac tgctccatca
     1801 ttggtttagc aataaatata ttttttaggt ctttaatgtc aatagtgtta aatatgccag
     1861 catatttatt tgatagtaat tctattgcaa atatttacag agtatttcct atatcattta
     1921 gctttatagt tacaggacta tatttcagtt ataaaaataa taaaaataat aagattaact
     1981 ttgaggcagt tttagaggac aaaaaaaact taattttctt attgagcctt atgtttggaa
     2041 tgtacttgta tctatgtttg agtttgtcaa tatactatac tgttggaaat gtattgattt
     2101 taaaattata tggaatcaag tctgccataa tagttttggt aggtaactat attgcagaaa
     2161 gatatagctt tagaattact caaataaaag gttatgatat taaaaatcaa atagaatatg
     2221 tagaaagtgt aaagacagaa tatgagatta ataaattgaa tgatattgta tatacagatt
     2281 tactgacagg tttctacaat agaccttttg tgaatcaaaa actatcagaa tggacatcaa
     2341 atcactacaa atttacctta tgttttactg atttaaatag cttaaaacct gtaaatgata
     2401 aatttggaca ccaatttggt gataaataca tatccactac tgctcaaata ataaaaaaac
     2461 ttataaatgt agaaaattcc ttactattta gatatggtgg agatgaattt ttattccttt
     2521 tacagaatac aagttgtgta gaagcagaaa agataatgat taatataaat tatgaattat
     2581 caaaattaag taatactgac gagtatcctt atgctctttc cataagctat ggattagttg
     2641 agtgggatgg aatatccgat attgaaactt taatatcaaa ggcagattct ttgatgtatg
     2701 aaaataagct taaaaataaa tctactttaa actagctagt taagatattc tacaaaatat
     2761 atgttataag agagtactaa gaatatcaaa atatatagat tttgcaaata ttgaatacag
     2821 tttactaatg aatgcactgg atgtaagtgt tagtaaacac ctattagatg aacactttac
     2881 tgttgtttgg gctaatgacc gttattatga aatgttcagc tatacctagc taaaaagtta
     2941 aaaatgacca ctgtagccga gggaatagag accagttttc aaaaagattt cttgcaagaa
     3001 ataaactgtg atatgttaca aggatacgtg ttctctagac ctttaccaat aaaagacttt
     3061 gaaaaactta tgtttcaaaa ctcctccaat taacaatata aaattgctta ttataagtga
     3121 tattattgta tcaacaaata aaggaattgt ctcaaaattg attttttcta atcaaatttt
     3181 tattttgaga cattctcatt tttttgcata agaaaatacc caccaagcta cggtttttct
     3241 taccgctgtt cggtgggtat tagtagtatt atgaaatctg aaaactttaa gtattactac
     3301 ttccgctaac gcaatcacaa aggtcgaaag cttcaaagcg aaaataggct ttttactcat
     3361 caaacgtcac cgtacctgcc aacccgtcca ccgttatcat ttgtccgctc ttaatagttc
     3421 gtgtggcaat gtgtgtagcc ataacggctg ggatgccata ttcacgtgcg acaatgcttg
     3481 aatggctaag aggaccacca atgtccgtta caatcgcact tgcagatgaa aataatggtg
     3541 tccatgctgg agttgtggta actgcaacca aaacacttcc aggttgaaag ctttcaaaat
     3601 ctgctggact attcagtaca caagctggag ccgtcacaac accagtactg gtaccaatgc
     3661 ccgtcaacac aattttccca tctttctgct tctgttgtgc ttgtgatata gtttttttgt
     3721 ttttctctgg caacttggat ggtgacatat aacccatgta ttttttaagt tcagcttttc
     3781 gaacaggtat tatcccgttc atatcagata gagacttgtt tttatccaac tgctctataa
     3841 gtacctccaa ttccgatttt gtgagccaat aaatatcatc taaatgtgaa atagctcctc
     3901 ctcgtatgaa tcgttctgag atttcctgaa acatacaacg aatcagagga tgtcccattc
     3961 ccatcaaata aatagcattt tcacgcatag gagaggtttc ctgtgcccaa tggagcaact
     4021 taaaaaatag ttttttacgc aatccaccta tgtgttgtaa aatctcttct tctgcatgtt
     4081 ttctacgctt ttcaaacgtg gtctggcgta aaaaaggact ttcccctttt ccttccacaa
     4141 atgtttttac ggattcaaaa gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat
     4201 caaactcata agcggtacga ccaaactctt taaagtactt attgattcga tttttccatt
     4261 ctatccatac ttcctgtgaa actccaacag gcacaactga agacatgaaa tcttctgcta
     4321 tctttattgc aggactgttt tgcagataaa ggttgatgct gttattttgc tttacccatt
     4381 ctgaaatacc ccacaactcc ttttccgatt gcagaacaat cgtatcaaac ccaagcagga
     4441 aaacggaagt atccgttata ccagcacgtc gaactgctcc ttgaaataat tttgtaaata
     4501 atgtttcact gatagaagca gcaggcaatg taagctgaat ccttgtgaaa taaatacatg
     4561 cggcataaaa tattgtctga ataccctcca taatttgatg ggcgttcata gtatgcaggg
     4621 gcttttcttc ccattgttta atagcatcct catatccttt tcgtgccgtt tcccagcgtg
     4681 caacgctgtt cataagtgtc ctacgtaaag agcgtgggga aagggatttg atagcaatca
     4741 aaagtggctt tgatttagca gaaagataaa catatccatt aataaccgta tatgccccgt
     4801 tttttggtaa taattttttc gcacttttac caaacatatc tatccataaa agctctgatg
     4861 cacggtttac gatttcaaga ccaagtgtgg cgaacaaagg tgtaacagga tttggcaaat
     4921 gttctgccaa acttcccttt gtgtatacaa catcctgttc tggcaatacc cattctggtg
     4981 gcagaacagt aattggacga gcctgaacaa tgtacaattt atctttttcc agtgcccact
     5041 ctacatccat aggcatttga taatacttct caattttctt tccaagctgt atcagttgca
     5101 ttacctgatt acgagtaaga gcatattttt ttctcaacct ttcaggaacc atggtttctt
     5161 ctgttccttc tgaagtacga accgtcataa tctctttatt tgccacttca tacgatataa
     5221 ttcgttcgga gtccttatcc actacgattg tatcgggtgt aactaagcta gaaaccaccg
     5281 cttcaccaag cccccatgca gcgttaataa tcatttcgct acgtctacca ttgattgggt
     5341 ttaacgtaaa cataatacca gatgaatcgg aaaatgcaag cttttgtact acaacagcaa
     5401 gtgcgacaat ctcgtgcttt atattattct tcacacgata agcaatagca cgagctgtcc
     5461 ataaggaagc ccagcatctc tttacagagt caataacttt atcttcacct tgtatgttaa
     5521 gataagtttc ctgctgacct gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg
     5581 aacggacagc caccgatatg ttccctaatc cagcataagc cattttaatt gcgtccgaca
     5641 cttcctgtgg catttctcca ttatggaaaa gctcccctat tttcttagat acatcttcaa
     5701 gttggctggt attattggaa tcaataccat ctagcagttt attaattcga gactgaatat
     5761 ggttcgtctc aacaaaaatc tggtatgaag cagtcgtaac atgaaaaccg tctggcacag
     5821 ggatgcctgc tgtcaatagt tttgataaag acatcccttt tccaccaaca atttcaatgg
     5881 ttgcttgttt atgttccagt ggcaagacat aataaatcat aatcattcac ctccatcatt
     5941 cataattccg ttcagaaata tattcattgt ttcatccaac attttttccg aactatccaa
     6001 tggtcgtgac gtgtagctga ttgataacat ggagttaatt aacaaacgtg tcacaagagc
     6061 ggtatccgtt ttgcgaaaga tgcctgtggt cacaccatta tcaattaccg attttataat
     6121 atcttgatat gcataacgag catctttaag tctctttcga tactcttctt ctaaataatc
     6181 agattctgta tttaaccaca tcagtacagt tctatactgc tctggaaatc taagatgatt
     6241 aagcatgatt tttctaaggc actgttgtgg tgaaggttca ccatcaataa tcctatgaac
     6301 ttgttggata gttttttcta tttctttctc aactgcatat acaacaatct cgtctttcgt
     6361 tttaaaaaaa tcgtatagac ttgactttcc catgttggct aaaactgcaa tttctctcat
     6421 tgatgttttt tggaatccat tttcagccat gagactcaaa gcaacagcaa ctattttttc
     6481 tcggcgttct gcttgttgtt cgggtgtaag cacaattcct tttggcatag tgcgatacct
     6541 ccttttagcg accactggtc gtttcttttt tatttatcat acagcaacca acggtcactt
     6601 gtcaaggtta atttttttca ttgtatatgg tactcgagat aaaaatgttc attttagaaa
     6661 ttactctatt taagtagaaa taggcttcat cattagaaag caagactata aatttgagat
     6721 atttatcatg ttcaaatttc atcaatatca aagtttaaaa tattaataaa aaaagaatta
     6781 ctaagaaata acatataaag attcaaaaaa ggagttgaac ttaaaattca attacaaaaa
     6841 atcaaattta agacaactcc tttattcttt atctctacta ttctttatct ctatttattt
     6901 attacaactc cctaacattt accactactg ccttaggtct agtcatagcc tcaattgaat
     6961 acttaacacc ttgagttccc ataccagatg cctttacacc taaaaatggg aagtggtctg
     7021 gacctctttc agtcttatta tttatttgaa cagtaccaac ttcaagttta tcagctatat
     7081 aaaatgcctt atcaatatca cttgtaaata ctgatgattg aagcccatac tctgattgat
     7141 tagcaatttc aattgcttga tttatatctt taactcttat aattggaagt actggaccaa
     7201 atggttcttc ccaagcaatt ttcatatcaa catttacatt atctaacaat gttggataga
     7261 ataaattatt ttttctaaca ttgcctgtta ttaactttgc tcctttatgt aatgcatcat
     7321 caaccaaccc ttgaacaaaa tcagttgctt tattatctat aagaggagtg ataactactt
     7381 catccattgg atttccaatt gtcaacttct ctaccaatgg ctttattttt ccgactaatt
     7441 tatccgccac actctcttgt actaaaattc tctttactgc cgtacatctt tgtcctgaat
     7501 atgaatacgc tccagatact atattcttag ctgcaaaatc taagtctgca tcttctaata
     7561 ctatagccgc atcttttcca ccaagctcca ataataatgg taccattcca gatattttag
     7621 atatatgctg accaacttca gtactaccag taaagttaat aaaattaatt cctttatgcg
     7681 ttacaacata atcccctatc tcactacccc tacctgtaat agtattaagt actccatctg
     7741 gtatcccagc ttttctcata atttctacca catgaagtgc gctaatagcc ccttgtgtag
     7801 caggttttaa tacgactgca tttccaccaa taagtgctgg agcaatcttt gacattgata
     7861 aatttacagg ataattaaaa ggagatatag ctaaaacagt acctaatgga acccttgaaa
     7921 cataagacat cttatttctt gttccaccag ggaaattatc tccactaatg gcttctccct
     7981 ccatattttt tcccacatca gctgtgtatc ttaaaaaatc tgctgttctc tcaacttctg
     8041 cccttgcaga ttttatgtct ttggctattt caagtaccag tatattagct atctcatcta
     8101 tattttctaa caataaatca gcagccttat gaaatatatt gcctctcttg tatacaggca
     8161 tttcagccca tacttttata ctctcttttg tgttttttat aacctcatct acctcatgtt
     8221 ttgtcataga ttgtactttc ccaacaagag aattatctat tggtgagtaa atttctattg
     8281 gctttctcga attagattct acccattttc catttattaa atttttatat acattttctt
     8341 ttgttttaag ttcattaaac atatttttat tcctctctct gatataactt tgtgtattta
     8401 aattatagcc atttattcgt tatgtaaaca tttctttttt tcataaacgt ttacctataa
     8461 aaaaatttta agttaacgtt tttctacatt cttctatttt aataacatac tcgctccaaa
     8521 tctatatagg tcacacttta atactaatat atcaatatac caatatacca atataccaat
     8581 ataccaatat accaatatac caatatacca atataaatat tataaacata aatcacttga
     8641 caaatttata agtagacaat attatatatc tatgactcaa aatattttta aagtcatata
     8701 tatcatatat agacagaaag gattgataaa aatggctaaa agttttacag aaatagaaaa
     8761 agacaatata agaaaaagac taatttcaga atgtgaaatc aactggtcta aatatggata
     8821 caaaaaaact aatattgatg aactttgttc aaaatcagga attgcaaagg gttctttcta
     8881 tacatttttt gattcaaaag aaaagttatt ttttgaagta ttaaatggaa tccaaaacag
     8941 acttatatca tcagtagatg aaattttaag taacatgcct ccaaaagaag gctttgcaaa
     9001 atccttaaaa atgttatatc gattatatga taaaaatcca tttttatatt caccaaataa
     9061 tcctgatttc atcagcctaa taaataaact acctcaagat atgtttttac aacttgagtc
     9121 caataacata caaagttttt acgacttaat tgagagacac aatctcaagt tgaagataga
     9181 aaaagaaaaa gtttttggtg tttgtaatgc tctactatca agtctgttgg taaaagaaaa
     9241 tattgaatat aactatattg aagtgtttga ctttatggtg gataatcttg tagaacacat
     9301 acttgaataa caatttaaaa taatttttct aagtattttt ttatactaaa taggattaat
     9361 atgattgaat taatgcaaaa aataaacaat ataatttata aaataaatga taaactaaat
     9421 atcacacaaa acttatagac cctagagcaa acccattcca ctctactcta agccatctat
     9481 aatagtttgt atatttctaa taatattaga tattaaagta agaaaaaaat tccttataat
     9541 aattgctatt atatcaatta ttgtaaatgt tttaataatc ttagataggt aggtacatat
     9601 atggtaaaat cctcgaacac ttttaataaa aacttcaact tgatgatatt aggtcaaata
     9661 atctcattgt ttggtgcatc aatacttaaa tttgctttat ctttatacat acttgatata
     9721 actggtaaag cagaaatatt tgctactata ctagcagtat cttcaatccc aattatagta
     9781 ttctccccta ttggtggagc tattgcagat atattcaaca gaagaaactt aatggttatt
     9841 tttgattttt caagtagtct tacagtacta atcttagcag tattcttatt caacaataat
     9901 ggttcaatat tacttgtagg aattattatg accatattat cagtcataag cacaatgtat
     9961 caaccaactg ttcagtcaag tacacccctt ttagttgata atgagcattt aatgaatgca
    10021 aatggtattg tagctggagt tgcatctctt acaaatattg caggacctgt attaggaggt
    10081 gtcttgtatg gtgtgattgg aattaatgca attatagtta taagttgtgt aagtttcttt
    10141 ctatctgcaa ttatggagat atttatacag attcctttta tcaaacaaga gcaaaacagc
    10201 aacattataa aaactatatt tactgatata aaggatggta ttgttcatat atgtaaagaa
    10261 aatagattta tactaaaaat acttttttta gctatagcaa taaacttatt tttaagctct
    10321 atgttaatag ttggtatccc ctatatagta aaaatcacta tgggagctag taacttctta
    10381 tatggtctag cagaaggagc aatttctttt agttcactgg tagctgctgt tagtattggt
    10441 attttctcaa aacatctaaa aatcactaat ttgcatattt cctttattat atgtgcagtt
    10501 atgttcatac caatggcggt ttcagtatat ccatttatat taaacacagg atttccagtt
    10561 ccatttttaa tctttatagt atcatctatg ctaatatttt ttacaacgac tcttatctca
    10621 atatatgtta taacagtcac acaattaaaa actccaaatg aactcttagg aaaagtaatg
    10681 tcaattcttt ttgcaggaac agcaattgca atgccccttg gtcaggttat gtatggtaaa
    10741 ctttttgatt tattatcaga tagagtgtac atcattatac taggagttgg atttattaca
    10801 ttacttatag catttgtatc aaagataaca ttgaggaacg aaaatcttta gatacaaaga
    10861 atttagatat aaagatttaa ttttcactaa tttctgaatt taaatgcaaa attgtgtatc
    10921 agtatgttga cgttttagaa attgattgat ataatcaatg gaaaataaac tttataaaaa
    10981 ttcaaaatat tcagctagag gagtgataaa aatgagttac atgccaactt tagaacaagc
    11041 atgggaaata ttaagaaaat acaataagga agaatttcat ataagacatg ctcaaattgt
    11101 atctggagtt atgagatact atgcaaagga gtacgaccct gaaagagtag aattttggga
    11161 aatagttgga cttttacatg atttagattt tgaaatgtat cctgatgaac attgtgttaa
    11221 acaaagagaa ttaatgacag agcttgattt agataaatca ataatcgatt ctacaataag
    11281 tcatggatat ggattaactg gttcagatgt taaaccagag caatttatgg agaaagtatt
    11341 atttgctgtg gatgaactta ctggtttaat tggtgctgct gcaataatgc gtccttcaaa
    11401 gagcgtttct gatttagaat taaaatctgt taagaagaaa tttaaagata aatcatttgc
    11461 tgctggctgt tctcgagacg ttataagaag tggtgcagaa atgcttggat gggaacttga
    11521 taagcttatc caaacaacta tagaagctat gagaagcttg attccagaga tggcaatata
    11581 gctcctccat acatatacca tagaagttac atctttaaag agcatttctc tttttgatgc
    11641 aacttctttt tatattttgt agaataaaaa gtttaatatt ttaaactcac tatataataa
    11701 ataatataat gtataatagt cataaacttt ctaaccttaa aagtttctaa taatcaataa
    11761 acaattctaa tatacaa
//
LOCUS       DAMJZE010000122        11642 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJZE010000122 DAMJZE010000000
VERSION     DAMJZE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.124"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            16..2193
                     /locus_tag="PC541_002720"
     CDS             16..2193
                     /locus_tag="PC541_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2840170.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="PC541_002721"
     CDS             2207..2629
                     /locus_tag="PC541_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2840171.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2803..3378
                     /locus_tag="PC541_002722"
     CDS             2803..3378
                     /locus_tag="PC541_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2840172.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(3503..4219)
                     /locus_tag="PC541_002723"
     CDS             complement(3503..4219)
                     /locus_tag="PC541_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2840173.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            4384..5109
                     /locus_tag="PC541_002724"
     CDS             4384..5109
                     /locus_tag="PC541_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2840174.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5169..6761
                     /gene="hcp"
                     /locus_tag="PC541_002725"
                     /gene_synonym="priS"
     CDS             5169..6761
                     /gene="hcp"
                     /locus_tag="PC541_002725"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2840175.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            7133..8038
                     /locus_tag="PC541_002726"
     CDS             7133..8038
                     /locus_tag="PC541_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2840176.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            8162..9115
                     /gene="msrA"
                     /locus_tag="PC541_002727"
     CDS             8162..9115
                     /gene="msrA"
                     /locus_tag="PC541_002727"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2840177.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            9268..10398
                     /locus_tag="PC541_002728"
     CDS             9268..10398
                     /locus_tag="PC541_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840178.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIY
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(10515..11483)
                     /locus_tag="PC541_002729"
     CDS             complement(10515..11483)
                     /locus_tag="PC541_002729"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2840179.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
BASE COUNT         4533 a         1324 c         2009 g         3776 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta taaacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttcgccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      841 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga
     1021 tttcaaaatt atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttccta attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca
     1441 tcatttgaga gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gctatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtgt aagacatatg
     1621 tttaaaaata catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggttca tatacaagag aagggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca
     1801 ctaccaacat atagaggatt taatgataat atagatgatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctaa ttgtgatatt tctagcttag atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaagctgat
     2101 ataaatagta ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgtaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg
     2401 tgtatgtcct aaaaattgtt ttaaaaaaga agaaggattc attgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgcc ctacaggagc attaaaattt aataaataat tgtattaaat
     2641 tgctatacta cttttaaata attgtgttaa gaattatttt ttgatatact tttgtaatat
     2701 aaaatgtaaa taaaacaaat acaaagtata taaaatatat ttttagaata aagtgtgctt
     2761 tagattaaat ttagaaaaat aaattagaca ggagaactta atatggtaaa agtattagcc
     2821 tttatgggca gtccaagaaa aaaagggaat gtagacacaa tacttgatga gataataaaa
     2881 ggtgtaaatg ataataatgt tgaagttaaa aaatattatc taaatgatat gaatattaaa
     2941 ggatgtcaag gctgtctata ctgtagaaaa gttcctactt gtgcatttaa agacgatatg
     3001 attgacatat atgaagatat aaaaagtgct gaatatataa taattggttc gccagtatat
     3061 atatgtcagg tatcagctca aacaaagcta cttttagata gattgtatcc tttaacagag
     3121 ataaataagt ctaaacatat acctaggttt ggtcaaaaaa agttaatcat ggtctatact
     3181 caagcagctc ctatttcgtt tttatttaaa aaatatttta agtatactgc aaaacattta
     3241 aagggcatgg gattaaatca ctacaaaact atagttgtaa ccaaagcatt tgaaagaaat
     3301 tccactaaaa aaaattttaa ggtattgaaa aaagcctata aacttggaat gaaaatagcc
     3361 attgatatca atggatagat aaaagtttaa tttatagtat agctaaagga gcattaatta
     3421 ttaatactcc ttttttgtat aaaggtttct agaaaataac taataaaaaa caatattatt
     3481 tgaatacaga tattatacaa gcctagatat ctaacttgtt ttgaaaataa aagatttcaa
     3541 atttattttt gttaaaagat aacaagcctt catctcgcat cttgccaagc tctcttgaca
     3601 tagaacttct gtcaacacat aaaaaatcag ccatttcatt tcttgaaaag ggaattgtaa
     3661 actggtggct acctgtttgt tctatctgtt ttgaaaaata cataagcaat ttctcgcgag
     3721 ttgttttacg agaaagtaat tctattttat tgtgaagtaa aatgtttttt tcagctatta
     3781 attgtagcat attttcaaca agcctattat gaaatacaca tgcagatgaa caaataccag
     3841 ttacactttt aaactttata aataaaactt cgcaagaaga tgttgtggtt actgtaacat
     3901 tacttttatg tagtctagta catgcgaata cctctccaaa taaatctcca ggcaagagtt
     3961 cagctacaat attcctattc ccttcgatat cttcctttat tatatgagca cttcctttaa
     4021 gcataattcc aatagaagta acttcacttc caacagagaa tattatacta tcttttgggt
     4081 aagtcttatc tacaacacct aaacattcca acatttcttc aagttcatca cttttaattc
     4141 cttcaaatag aggaattttt ttaagtaagt ctatatgctt ttctaaaaat tttttcaaat
     4201 aatcacttcc ttgttgcata tgcaaccgat aatttgtttt cattgtacta tactaagttt
     4261 ataaaagcaa ggggatataa aaatcctact acaagaaatt tgaaataaag aaatataggt
     4321 attaaaaata aatatataag aaatcaatac tttaaattaa aaaaatttta tgaggtgata
     4381 taaatgatga gaaaaataat taaaattgat gaggaaaagt gtaatggttg tggactatgt
     4441 gttgaagctt gtcatgaaga agctatagga atggtaaatg gaaaagcaaa acttttaaga
     4501 gatgactatt gtgatgggct gggagattgt ctgccaactt gtccaacaaa tgcgatttca
     4561 tttgagtata gagaagcagc agaatatgat gaagctgcag taaaagcaaa tatggaagca
     4621 aagaaagcac aaaagaaaac tttagcatgt ggatgtccag gttctcagtc taagtcaata
     4681 aatagagagg tttcaaatag tacgtctata agcaatgata tagtagagat aaaaggttct
     4741 caacttaatc aatggccagt acaaataaaa ttagtaccaa ctaatgcatc atatttaaag
     4801 aatgcaagtt ttttaatagc agcagactgt acagcgtatg catatggaaa ttttcataat
     4861 aaatttatga aaaataaggt tacattaatc ggatgcccta aacttgatga agttgactat
     4921 gctgaaaaac ttacagagat tttaaaagaa aatgatatta aaaatatagt agtaacacgt
     4981 atggaagttc cttgctgtgg tggtatagta aatgctgtta agacagctct acaaaatagt
     5041 ggaaaaatga taccatggca aatagttact atatctgtag atggaaaaat tgtagatgga
     5101 gaaatttaat taagtgaatt atagaaaaat ataataaatt ttaataaata aaaagggagg
     5161 aataaaaaat ggaaaaaatg ttttgttttc aatgtgaaca aacagcagga ggtaaaggtt
     5221 gtactataaa tggtgtctgt ggtaaaaagg ggggtacagc taacttacaa gacgaactta
     5281 caggagtgtt gattggtctt tcaagagcaa ctattggaaa taagaataga ccaacaagtg
     5341 aaacagataa aattatgata gaggggctat tcactactat aacgaatgta agttttgatg
     5401 atgaagctat aaaaagacaa attgaaaaaa ctgaggtaga aaaaagtaaa cttgtaccta
     5461 gatgttcaga ttgttcaaca acttgtaata gaaatgataa ctatgatatg aaggatttat
     5521 ggaatgataa tgaggacata cgttcattaa aatcattaat cttatttgga ttaagaggta
     5581 tggctgccta tgcatatcat gcaatggtac ttggttatac agacaaagac gtaaatgaat
     5641 ttttctatga aggattaata gcagttggag gagacttgtc tattgacgaa cttttaggtc
     5701 tagttatgaa aactggagaa ataaacttaa aatgtatgga attgcttgat agagcaaata
     5761 ctgaaactta tggaacacca gaaccgactc aggtatctat gaaaatcgag aaagggccat
     5821 ttattgtagt tacaggacat gacctttatg atttacacaa attattagaa caaacgaaag
     5881 ataagggtat aaatatatat actcatggtg agttattacc agcacatgca tatccaaaac
     5941 taaaagaata taaacatcta aaaggtaatt ttggtacagc atggcaaaac cagcaaaaag
     6001 aatttgataa tattcctgct ccaatattat ttacaacaaa ctgtttaatg cctgttaaag
     6061 atagttatag agatagagta tttactacag aagtagttgc ttatccaggt atggttcata
     6121 ttgatgaaga taaagatttt tctagtgtaa tagaaaaagc tcttgaactt ggaggatata
     6181 gagaagatgt tcatatgact ggtattaatg gaggagatac attaactact ggttttgcaa
     6241 gaggtacagt ttcatctgta caagataaag tagtagatgc tgtaaagagt ggagctataa
     6301 gacacttttt attggttgga ggttgtgatg gtgcaaaacc tggaagaaat tattacactg
     6361 aatttgtaaa acaagctcct aaagacactg ttatattgac acttgcatgt ggaaaatata
     6421 gatttaatga tttagacctt ggggatatag gtggcttacc tcgtttaatg gatatgggac
     6481 aatgtaatga tgcttatagt gcaattaaag tggcagtttc attggcaaat gcttttgaat
     6541 gtggagtaaa tgatttgcct cttagcatgg tactgtcatg gtatgagcag aaagctgttt
     6601 gtatacttct tacactactt catttaggta tcaaaaatat ctatataggg ccttctttgc
     6661 cagcattctt atctaagaat gtattagata tattggttga taaatttgca ttgacaccta
     6721 tatctactcc tgaagaagat atgaagaaga ttttaggata aataaacata tctatactta
     6781 aagatattta tagttaaaat atttttaagt atgccacaat taagataagg ggttaaactt
     6841 aaaatttgat taaaaaatca attttgagat gaccctttat ttattttttg taataattga
     6901 aaaataaacc tgctttcagt atattctttt taagaatctt tgtattaaat ttataatact
     6961 tagatatata atatcttata tattttattt taagtattaa aggtttgaat gtaacttaaa
     7021 tattaaaaga ggaacattgt aatatgtaac ttgcataaaa tatatatact atagtgaaat
     7081 tattttgatt aaaatttaaa atataaggtg ttttatgtgg gggtatatgt atatgaaaaa
     7141 ttctaatttt aatatattgg catttgatgg tggtggatta aaaggggcat tgagtatatc
     7201 catacttgag aggatagtaa aagagtatcc aaatctatta aacaatataa atatgtttgg
     7261 aggtacatct acagggagtc taattgcact agggcttgcg tatggtgtaa gccctaaaga
     7321 gataaaagaa ctttactcaa ttgaaaattc aaagtatatt tttaataaaa gttatgcaga
     7381 aatactcaga ccaaagtatg aaaacaaaaa cttaaaagaa gttttattaa gtatatttcc
     7441 agaggaacta gaattaaaag atttaaataa attagtaatg ataccaagtt tttatatagg
     7501 aaatgaggaa aatgcatgga aaccagtgtt ttataacaat atgccaaatt cttttacaaa
     7561 gactagcaaa gttgtagatg tagcaatggc aagtagtgct gctccggtat tttttccaac
     7621 atataatcgt catgttgatg gaggaataat agcaactgac ccgagtttag cgtgtataat
     7681 tcatgccatt gacagtggat ttaaacttaa gaatacaaga cttttttcaa tagggactgg
     7741 atatgtatat aacagtataa aggcagatac taccgaatgg ggagctattg actggataat
     7801 taataaagaa cccgatttac caattatatc tattacctta gaaggaaatt ctcaaatgag
     7861 tcagttattt tctcaaaaac ttcttggaga taattactat agattaaatc ctaagatgga
     7921 aaaagatgta gctatggatg attgtgatgc attagaatat ctaatgtctt taggagaaaa
     7981 ttgtgatata aaagatgctt ttggatggat aaagaaaaag agcttttata gaaattagat
     8041 tcaatttgca attgtatttt tacacatgat ttcattgagt aggtaaaaat aataaaatgc
     8101 agattattaa gttacttctt agggaataaa taaagtataa tttatttaag gagtgatgaa
     8161 aatgataaaa cttgctactt tttcaggtgg atgtttttgg tgtatggtaa aaccttttga
     8221 taagtatgat ggagttatta aagtagtttc tggatataca ggtggtcatg ttgaaaaccc
     8281 tacatatcaa gaggtatgct caggaactac tggtcattta gaagcaattc aaataacata
     8341 tgatgatgat ataataagtt atgatgaact tctaaatatc ttctggaaac agattaatcc
     8401 tacagatgat ggtggacaat ttggtgatag gggaactcaa tataagactg ctatatttta
     8461 tcatgatgaa gaacaaaaaa atatagcttt agagtcaaga gaacatctgg aaaatagtaa
     8521 aatatttgat agtaaaatag tgactgatat aattccagca actaaattct acgaagcaga
     8581 agaaaatcat caggattatt ataagaaaag accattaaac tacaatatgt attataaagg
     8641 gtctggaaga tataattttg taaaaagcca ttgggatgga aacaatataa atagggaaga
     8701 gttaaaaaag actctaactc ctatacaatt tgaagtcact caaaatgata tgacagaagt
     8761 accttttgaa aatgaatatt atgacaataa caaagaggga atttatgttg atattgtaag
     8821 tggagaagtg ttattttctt caaaagataa atttgattct ggatgtgggt ggccaagttt
     8881 ttcaaagcca gttatagata aatctatcat tgagagaagt gatttctcac aaggtatgta
     8941 cagagttgaa gtaagaagtg ctaaaggaaa ttctcattta ggtcatgtat ttgatgatgg
     9001 tccaaaagaa cttggaggtt taagatattg tataaattct gcttctctta aatttatacc
     9061 aagagaaaat atggaagctt taggatatgg agaatattta aaactttttg agtaattagg
     9121 aatatttatt ataaacttat ctaaaaggtg aaaatataaa aaaatatttt caccttttgt
     9181 ttttttgtca ataatatgat aaattattaa ttgtaatctt atttatacca atagattaag
     9241 aatttaattt tgaaagagag aaaacaaatg aatttaaaag actttaatgg aaagagatta
     9301 aaatcagcta gaatttttag agcaaagacg atagaacagt tgtctaaaga aactaaaata
     9361 aataaaaaag atttaaaagc ttttgaggaa aataagtatg taccaaatat agaaaataca
     9421 ttaaaattat ctaatatatt aaattttcca aaagagtatt tttataaaaa tgaaaatata
     9481 aatatcgttg tagaggatag ccactttaac cctcaatcta aactacctag agtagaagag
     9541 atttcttata gagaaaaaat aattataata tataaaatat attcatttat ggagaattat
     9601 attaagtttc caaaagaaaa cttgccaaat agaaaagtaa tgtcagatat taatataaat
     9661 gatatagaat ctcttgcata taagactaga gagttttggg atttaaatag tactccaata
     9721 gtaaatatgg taagtttgtt agagtctaaa gggattataa tatcaggtat gaatgtagat
     9781 agaaaagggg caactatctt tactcaaaaa cagagaatta gtaaagaatc taaatatcta
     9841 atatctctgg gtaatgacaa aaagtcagca tcaattagaa attatacttt ggcatgtgag
     9901 cttggatata taatatcaag tgaattaaga ataccaagta agcaattttc agaagatgag
     9961 tatgcctgtg catttttatt gccaaaagag agttttttaa aagatttgac tcatccagaa
    10021 gatttagatt attatgtaga actaaagaaa aaatggattg tacctatatc tgctatgatt
    10081 tttagagcat ataatctaga aaagataaat tacaaaaaat acaattatct tatgaatgaa
    10141 atggataaaa aaggctggtt gatagaagaa ccactagaca aaatgaaagg ttcaagccca
    10201 acgtatttaa aaagagcagt tgaattattg atagaaaata aaataatgag tgtaaatagt
    10261 atagtatcaa gtttagagga atttggaata aatctttatc cagaagactt agaattattg
    10321 atgggattga aaaaaggatt gttatctcaa gaggtaaata aaaaaagtaa agttataaaa
    10381 tttgatggta aaaaataggt ttatatttta gtattaaaat taaaagcagg tacaaatgat
    10441 ttaaacatta acttagttaa aggtcttttc aataagtcat ttacctgctt ttagtgtaag
    10501 aaagaatatt gaatctaaag tgtttttgaa agtgcaatat attcccttaa aacagaacaa
    10561 gatttattaa attcattttg ctcatattca gtcatattga tttcaataat ttcttcaata
    10621 ccatcttttc ctataacaca tggaacacct gcaaatacat ttctttcgtt atattgacct
    10681 tctagtaatg tagaaactgg aagaactttt ttctcattgt gtagtatagc ttttacaatt
    10741 tctactaaag cagtacctat accaaattct gtacaatctt taccatttat tatacttatc
    10801 cctgtttttt tatttttttc aactattgat ggtaagtcta aattgctata agtacttggg
    10861 ttgtctttta tcatgtctaa gattggttta ccaccaattg aaacatgaga ccaaggaacc
    10921 atttgagaat ctccatgttc acccatagaa taagcttgaa ttgattgttg agcgattcca
    10981 gtttcttcag ataaaatcct tctaagtctt gttgaatcta aagctgttcc agttccaatg
    11041 actttgtttt taggaaagcc agatttttcc caaatataat ttgtaactac atcaacagga
    11101 ttagatatga ctacaaatat accatcaaag ccagaatcaa ctatgggttt tactatagat
    11161 tttatcattc caatagttgg tcttagtaaa tctaatctag ttgtgttttg tctacgtaaa
    11221 ggcccttctg aagagtctgc aacacttatc actactatgt cagaatcttt acagtcagaa
    11281 aaagttcctt tttctatatg tactttatgt ggcagatatg aaactgcatc agctaaatct
    11341 aaagcttgag cttttgattt agattcatct atatcaatca taaagagttc atcacaaact
    11401 ccttgagtta ttaaactaaa tccacaatga gaaccaacat gccctgaacc tacaatagat
    11461 atttttcttg gctttattgc cattttaaat cactccccct aaaataacga tacattgaaa
    11521 attatagatg ttattaaaag ctgtttttgg atattttaat attctattat ttcagaaaat
    11581 ttatttaata tgattttata ctaaaatttt taaaaaagca atgttaatat actctctatt
    11641 ag
//
LOCUS       DAMJZE010000123        11594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJZE010000123 DAMJZE010000000
VERSION     DAMJZE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.125"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            101..664
                     /locus_tag="PC541_002730"
     CDS             101..664
                     /locus_tag="PC541_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840180.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            1061..2761
                     /locus_tag="PC541_002731"
     CDS             1061..2761
                     /locus_tag="PC541_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840181.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2931..3548
                     /locus_tag="PC541_002732"
     CDS             2931..3548
                     /locus_tag="PC541_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840182.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3909..5102
                     /locus_tag="PC541_002733"
     CDS             3909..5102
                     /locus_tag="PC541_002733"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2840183.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5120..6412
                     /gene="lysA"
                     /locus_tag="PC541_002734"
     CDS             5120..6412
                     /gene="lysA"
                     /locus_tag="PC541_002734"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2840184.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            6629..7288
                     /locus_tag="PC541_002735"
     CDS             6629..7288
                     /locus_tag="PC541_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2840185.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(7443..8528)
                     /locus_tag="PC541_002736"
     CDS             complement(7443..8528)
                     /locus_tag="PC541_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2840186.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            8719..9435
                     /locus_tag="PC541_002737"
     CDS             8719..9435
                     /locus_tag="PC541_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2840187.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9493..10071
                     /locus_tag="PC541_002738"
     CDS             9493..10071
                     /locus_tag="PC541_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2840188.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            10465..11337
                     /locus_tag="PC541_002739"
     CDS             10465..11337
                     /locus_tag="PC541_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2840189.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4786 a         1207 c         1985 g         3616 t
ORIGIN      
        1 aaatcaaaat ttgtaaaacc acatttatta tgttagaata tcttatgata ttaaattgta
       61 agtgtataat attatttagt tactagattg gggggaaatg atgaataaag tctatattgt
      121 aacaacatat acaggaacta tattatctta cttaataagg aatataagta aaaagctgta
      181 tacacatgtg tcaatatcat taaatgaaaa tctaaaacca atgtattctt ttggaaggct
      241 aaatccaagg aatccattta ttggaggatt tgttgaagaa aatataaatc aaggattata
      301 tgctataaga aaaaacactg tgtgtagggt atattcgtta gaagtagata atttacaata
      361 tgaaaatcta tataaaaata taaaattaat tagtgattat agagaagatt actattacga
      421 tacaatggca ttaatatatt tactaatgaa tattcagaga caatctgaat ataaatatgt
      481 atgttcaaat tttgttgctg acatgttaga aaaaagtgag atagacattt taaataaaca
      541 agcatttgaa gtaacgccaa atgattttta taatttatct ggcttaacct tagagtatga
      601 aggtttatta tcagaatata attctagaca acattcttat gtatcaaaaa tcgcaaacat
      661 ttaaaaactt gatttagagt ttttcatata aaaaaatatt gctagataat taggctgaag
      721 atagtagttt aacttcgata ggattaagaa tttgtaaatc ttagttagaa tactttcttc
      781 agtttttaaa tttctgctaa tatttgtatt tttataacat ttttggatat atataataca
      841 gaatagtgca aaaatcccac tcctttaggt gcgggatgga tagcacaaac agtgtataaa
      901 aagtatttat acatacacgt ataatgttaa atatagttga ataagcagaa ctataaatat
      961 ataaagatat cttaatatat aataaatatg ataaatattt attttttgta aatatatgga
     1021 tataatagta ataataacat tttttagaga ggagtattaa atgaaacgat atcttaaaac
     1081 atatggagtt gcattaataa ttgtaataat ttatgcattt attaaattac ctgtactaag
     1141 attagatttt acatctttat tttcagtagg aataattttc tttgttgtag caggaatact
     1201 agatatgatg ttagatagaa atgaaagagc atctaaaatg gctaaatata atttttcaat
     1261 agcaatagct ttgcttgtgt atattgtagt agttcccttt ataacttcta cacctgtact
     1321 tcatgcaaaa tcatatagag aactattggg aaaagtaact gaaagtaaat ttactgatga
     1381 cattagccct gtcagtgtaa atgacattcg tcttgtagat gaagatatgg caatgaaatt
     1441 aggtgacaaa aagcttggag aagttcctgc aatagggagt gtatccaaat taggaaaatt
     1501 tcatatacaa aatgtagatg gagagttgta ctgggtagcg cctcttgttc acagagacat
     1561 aattaaatgg ataacatctc taagtggtac aagtggttat gtaaaagtat cagctagtaa
     1621 tcctcaagac gtccaattag ttcaagaaat agatggaaag ccaataaaga tagtttacca
     1681 accagaagcc tacttacatc aggatttaca aagacattta tacatacatg gtattgtaaa
     1741 tgttggtatg acagatttca ctcttgaaat aaatgatgaa ggaagacctt attgggttgt
     1801 aagtttatat gaacataaaa ttggctatgg aggagcaaat gcaacaggaa tagccactgt
     1861 tgatgcagaa actggaaaaa taaatgttta tgatgttaaa aacacgccta aatgggtgga
     1921 tagaatacaa ccacaaagct ttgtaacaga ccaaattaaa gactggggtg tttatgttaa
     1981 tggattttta aactctgtta tatcagaaaa gggagtttta gtaccaacag aaggtacatc
     2041 attagtttat ggcaatgata acaggtctta ttggtataca ggtataacat cttcaggtgg
     2101 agatgaatct acaattggat ttatgttagt tgattctaga acaaaagaag ctagattata
     2161 taaacaacca ggagctactg aaacggctgc aatgaaatca gctgaaggta atgttcaaga
     2221 aaaaaattat gaagcgacgt tccctgtaat gtataatata ttgggtcagc caacatatgt
     2281 atcatcatta aaagataagg cgggtcttgt taagagagtt gcatttgtat ctgttgaaga
     2341 ttataatgta cttggaatag gtgaagataa gaatgaagca ttaagaaact ataaggatgc
     2401 attagaatct aagggtaatg atttaaaact tgataatgat ttaaaagatg aggtattaga
     2461 aggcacagtg acaagaataa gcccagatgt gcgaggtggt aacactaatt attatgtaac
     2521 tttagattca aataaagata taatatttag ggctacttca aaagtatcta gcgaattgcc
     2581 attgactcag gttggtgata aagtcaaaat atcgtattca aaagaagaat ctggtgtaat
     2641 agaaatgtca gaatttgata acctaaatat tgcaaacttt gaagataaaa aagcaaaaga
     2701 aaaaacagga actgatgaaa atacagattc caaagattct gaaaatcaag gtaatgtata
     2761 atttaaataa aaagattgat aatagtattt aacatagtga tagctaattt ttatagaatt
     2821 taattctata aaaattagct attttatttt aattctttgt aaatttaatg tcataaaatt
     2881 aatataatat tagtacaact ttgcaaataa tacttcaagg agaatgtact atgacgaata
     2941 tggaaaaaga gatattactt ttaattagca gggttagatg tgttacagag tctcaattta
     3001 gaaaaattta tggagaccaa aaaagataca ataaaaaaaa ttttaaaaaa actttgagaa
     3061 aaatgtgtag tgaatataca ctaaaaaaat atccttgtga tgtggattat tataattata
     3121 aagacctctc ttatgtatac tatttaaacg gtagtaaact ttttaaggga gatgatttaa
     3181 taaaggctct agtaggatca gaattagttg ttagaatcaa tactgcaggt tgtgatgtaa
     3241 aaagatttta ccgaaatgtt agtgtagatg atgtgaaata cgatttattt attgaatata
     3301 cagaccaatt tggtaatata aagcaagttt tagttgatgt tgacttaaat aatgaaatag
     3361 acatttctaa atacgaaaat ctttctaaaa aaatagataa atctacaatt ccatttttta
     3421 aagtaccaaa cgttttggta gttacaccag ataatctatc cgaattaaat gatttagaga
     3481 gatatgataa tgagtatagc ataaatttta tagatttagg attaaataaa ctatttaatt
     3541 gtatataagc taactttaga aaagaaaact atcgatttgc cacattttgt aaaataattg
     3601 ttgcaatatt atatagaata tgataaaact atattaataa attaattaaa aataatgagc
     3661 ctagggtaga ggtagctgta ttcaatagta ttcaatagga gctggtaagc gttgataatt
     3721 gatgaaaggg tatatagccg aagaaatttt gttgacggac aaattttctg gacttatgca
     3781 gaatatgcat aaggctgtca cttttaaagt ggtgagctac aaggttactt ggtctatgaa
     3841 atttacacgt ccaaagtata cctttgggcg tttttattta tcaaaattta gcttgagggg
     3901 gagaaagcat gagtataatt gttcaaaaat atggtggaag ttcagtagca gatactgaaa
     3961 aaataaaatc aatagcagaa aatataatag aacgaagaaa agaaaatcca caaatggtaa
     4021 tagttgtatc tgccatggga aaatccactg atgaatatat aactttagca aaggaattga
     4081 gtaatgaacc tagtaagagg gaattagatg ctttaatgtc tactggtgaa atgatttcag
     4141 catctttatt atcaatagca ttaaatgcac ttggatgtaa agctataagt tataatgctt
     4201 accagttaaa tataaaaacg agtggtcttc atggaaagtc tcaaattgat gatataaacg
     4261 taaacagaat taaaaatagt ttagatgaag gaaatgtcgt gatagtaact ggatttcagg
     4321 gaatcaacga agatggtgat gtaaccactc ttggaagagg tggttcagat acatctgctg
     4381 tagctcttgc ggttaaatta aatggaaaat gtgagatata tacagatgtt gatggaattt
     4441 attttacaga cccaagaaaa tattcaaagg ctagtaaact tgatgagatt gaatatgaag
     4501 aaatgcttga gttagcgagt ttaggagcac aagtaatgca ttcaagaagt atagagcttg
     4561 cacaaaaata tggtgttgaa atctatgtcg gacgtacatg tggtacagaa aaaggaacat
     4621 atataagagg gggaaaagac atgaaattag aggataaagt aataacagga ttagctacta
     4681 gtgatgatga cagttctata acaataaaag attttaaagc tgaaaatata tctagcttat
     4741 ttgaagatat agcaactata ggtataagtg tagatatgat aagccaaact gcacctattt
     4801 tagataagat aagtgtatca tttacagttc caaaagaaga gttgggagaa tgtaaaaaga
     4861 tagtatctaa atatacagat gaagaacatt tagtgattga taataatatc actaaatttt
     4921 ctttagtagg tcttggtatg aaaaatactt ctggagttgc tgctaaagta tttaagatat
     4981 tcaatgaaaa tggtataatg ataaaactta taactacatc agagattcgt ataacttgtg
     5041 ctataaattc tgatgataag caagtagcta tagaaaaaat agcagaagta tttaatatat
     5101 aaaatttggg gaggctatta tgaaacttca tggaactatg actgtaaata atgatgaatt
     5161 atatattgga ggagtgagtt gtttagaact tagtaaaaaa tatcaaactc cactttatgt
     5221 atttgatgaa gctttagtta gacaaaattg tagagaatat atagaatatt ttaaggctag
     5281 agagggaaaa aataaggttg cttatgcagg gaaagctttt ttacctcttt acatgtgtaa
     5341 tcttataaat gaagaacaaa tgtgtttaga tgtagtttca ggaggcgagt tatatacagc
     5401 ttataaatca aattttccta tggaaaaagt tttctttcat ggaaacaata agactaaaga
     5461 agaaataaac atgggattag atttaggtgt aggtaaattt gtagtggata atttttatga
     5521 attagatttg cttgaagcat tatgtgaaga taaaggtaaa actcaagaga tatactttag
     5581 aataactcct ggtatagaag ctcatacaca tgattatata aaaactggac agatagattc
     5641 taaatttgga tttgctttag taaatggaga tttatttaga gcaatagaaa aattaagtga
     5701 atataaaaat attaaacttg taggtcttca cgctcatata ggttcacaaa tatttgatgt
     5761 agaaccatac atagatacag tggatataat gatgaacttg gtaaaagaaa taaaagaaaa
     5821 attcaacata caactaacag aaattgacct aggtggagga gttggcgttt attatagcga
     5881 aggagataaa cctaaaacta taaaagagtt ttgtgaaacc ataatcacta aagcagaaac
     5941 ttcatgtaaa gagcttggaa tagaagtacc tacattggta atagaaccag gaaggtcaat
     6001 agttgcaaat gcaggaagta cattgtatac agtaggttct ataaaagata taaaagatgt
     6061 tagggtttat gtaagtgtag atggaggtat gacagataat gtaagacctt cgttgtacca
     6121 agctagttat gagtgtagta ttgtgaataa aattaatcat gaggctatga atcatgtaac
     6181 aatagctgga aaatgttgtg agagtggaga tatattaatt ggagatataa acataatgga
     6241 tattaaaagt ggagatatac ttataacaac atcaactggt gcatatggat attcaatgtc
     6301 ttcaaattat aataaaatac caagagcagc agtggtatca gtatttgatg gaaatgataa
     6361 gataatttgt aagagacaaa gctatgagga cttattagct ttagagatat aaaatagaga
     6421 agtatataat aaaaattaaa tattaaagtt tattatgagc agatttgcta ggatatgtat
     6481 ctctaaatta atttttggtt tattgtatcg ttcgtagcat atctgcttat tttttgtttt
     6541 actaacaaat ttcttgtaat aaatataaaa tattttaaaa actttataat tatgataaaa
     6601 tttaaagtat atgacaagga gggatttaat gagaggaaga gtaattttat taatatcgat
     6661 attatctata tttcttgtag gatgttcatt taataaaaaa gatgaaataa atttagtaga
     6721 gcaaggaaaa acatatttag aaaaacataa ctataaaaaa gctatggaat cattatcctc
     6781 tgcactggaa gaagatagta ctaatgagaa tgcaagagct atgtatatgc aagctatgag
     6841 aatgtcaaat atgacggagt ttgaagaatt aaaaaattat gagggtgcaa tcaaggaact
     6901 taaacttata gagaaaatca aaaatggttc ctctgttatt aaagaagagg caactaaaaa
     6961 gaaagaagag ttaactaaat tagaaaaaga acaaaacgaa gcagaggaac aaagaaagaa
     7021 aaaggcaaaa gatacggctg gagaagatag gtatagagtt gaatcagatg caaggaaatc
     7081 ttcttatagt ggaagtagta aaaataataa atctagttct aaaaacaatg ataaaaaatc
     7141 agatagtaat aagtctgaca ataataacag ttcttctcaa aatcaagaaa agccaaataa
     7201 tagtaatcca acaccagcac caactccaca agatactact ggtggttcag atagtggtgg
     7261 aaattctcaa aactcaaata atcaataagt ataaaatata tgcataaatt taatgaacaa
     7321 attatatatt ataaaaaata tctcttagca attttttctt actaagagat attttttgag
     7381 aaataattta ataaatagca gttaaatttt aaactaaaaa ttaaagtcac agtagagata
     7441 atctattgta aacgattatc tatatattta tcaacataag tttttacaac agcagctatt
     7501 ggtactgaaa gaaaaactcc tatgaaacca aatagagatt gtcccaccaa aatagcagat
     7561 attacccata tagctcctat accaacttga ttacccataa cttttggttc tataaaattt
     7621 ccatctaatt gttgcaatat aaatagaaat gcaatgaccc atatagtttt ttgtggacta
     7681 tataatatag tcatggctat tggagggatt gcaccaataa aaggaccaaa atagggaatt
     7741 atattaccta agaatattat tgaagctaaa aataaagcat tttctattct gataacaaat
     7801 tgaaatccta aaaatgcaat tataccaata ataagagagt ctaatattct accaagtaaa
     7861 taatcataga aaatatcatg agtagtccta aaaaattcta taatgttatc tgccttgttt
     7921 ctagcagtag aagaatatag aaatctctta aatcccaatg caattttttc tttgtcatat
     7981 ataatgtata tagatagtat aaatcccatt gtcatattaa atagcataga agatatacta
     8041 agtgtataga ttaagaagtt tttaggaatt ttaccaacaa aattacttgc ttcttttaat
     8101 aaattatcta aactatgttg tatatgagga attaaagttt ttagaaaata tatgtttgac
     8161 atattttttt ctataaaact atctatataa tcagtataca taggcatttc atttatcaat
     8221 ctatttaatg tattagctat tgatggaatt aataacctaa tggcaaaggc taaaataaaa
     8281 cctataaata tataagataa aaaaatggct aaaagtctat gtgctttgaa ttttatctca
     8341 aaaagcatca caagtggatt taaaagtaaa gcaagtaaaa tagctaataa aaatgggcta
     8401 aaaaacttta agaatccttc aatactagat ataaagtctg atggagtatt aataaattta
     8461 aaaaataaaa ttccaataaa cacgactatt aaataatatt ttatattttc ctttgaaata
     8521 ataatcactt ccttaaaata gtgtttagtc agctattaca tattaatagt atgtgaaaag
     8581 aaattacaat tatttaggaa aattaaataa tttttttgct tgtttttaat taaggtctag
     8641 tatagtataa taatgtagca aaattaacat tgataaaaat attaaacaca cataatttaa
     8701 gataaggaag gattaagtat gagaattaat aagtatatag cttcttgtgg aatagcatca
     8761 agaagaaaag cagaagaaat aattcttgga ggaagaataa aggtaaatgg aagtgtagta
     8821 aaagagttat cttttaatat tgatgaagaa aaagacatag tagaatttga taataaaaaa
     8881 gtaaaaccaa gtgaaaatta tatatatata gtattaaata aaccagaggg atatataact
     8941 acagtaaaag accaattcaa tagacccagt gtaatagaca tacttaaaga tgtaagggaa
     9001 agagtatatc caatagggag attagattat gaaactagtg gtttattaat acttactaat
     9061 gatggcgatt taacatataa gcttacccat ccaaagcatg aaattgataa aatgtatgtg
     9121 gcaagtgtga agggaattat aagtgatgat gagataagac aattcgaaac aggactaaaa
     9181 atagaagatt acacaacggc acctgccaaa ataagggtaa ctaaagaaaa taaagaaaaa
     9241 aattattcag tgtgtgaaat aactatacat gagggtagaa atagacaagt aagaaaaatg
     9301 tgcaaagcta taaatcatcc agttttaaat ttaagaagga tttctgtggg gaaaatagta
     9361 ttaaaagaca ccaaagttgg agaatataga tacctaacag aagatgaaat aaaatattta
     9421 aaatcaataa aataagataa aaataaattg agaaaaaatg aaataaagtt aatagtaggg
     9481 agaaatagta ttatggcata tgcaaagtat ttaactaaaa ttacagatat aaataaattt
     9541 tatttagaaa atattataaa tgaaggtgac atagtaattg atgctactat gggaaatggg
     9601 tatgatacaa ggtatctagc tgagaaggtt ggagaaaagg gctttgtata tgcttttgat
     9661 atacaagaag aagcaataaa gtctacaaga aagaaactag agaaagaagg atatacagat
     9721 agagtaaaat tgatatttga tgggcatgaa aaaatgaatc aatatataaa agaagaagtt
     9781 tcatgtgttt tatttaattt gggttatctt ccaagagcga aacattcaat tataaccaaa
     9841 ccagatacaa ctttaaaggc tataaagtta agtttagaaa tgttaaaaga aaatggagta
     9901 ataagcattg ccatatatac agggcatgat ggaggtcaag aagaaaaaga ttgcatatat
     9961 aaccttgtca ataatttaaa tcaagatgaa tttaatgttt tagaatctaa gtttttaaat
    10021 caagtcaata acccaccaca attgatttta attgaaaaga aaaaagcata ataaatacta
    10081 aaaaacttga caagattgta ttaagagtta atatatatag tgtgacctat taaaaatact
    10141 ttaaataata agtatacaac agtttattct caaaacaaaa tatacatcta aatatgcaaa
    10201 ataagataga taacttttaa atgagtaaaa attgaaaaat ttaaaagaaa tattggataa
    10261 atattaaaaa atgcaaggaa agatggtata ataaatttaa tcgaaaatac tatgtgaagt
    10321 ataaggtaca gaaaagttag aattttatga gtttaagaag ctatgataaa aactaagatg
    10381 aaattgagat aaaaaataaa atttttagag aaaaagacag tgttaataaa acaaaatagt
    10441 ttatataact aaaaggagaa aaaaatggaa gaaacaaatg aaatgaagga cagtaaacat
    10501 ttgatatcaa atatacaatc tcaatatacg agattgagta agggtcaaaa acttatagca
    10561 caatacattt taaataatta tgataaagtt gcatttatga ctgcatgtaa attgggtgaa
    10621 accgtaggag taagtgagtc tacagttgta agatttgcaa atgcattagg ttatagtgga
    10681 tatcctaaat tacaagctgc acttcaagaa ctcataaaaa ataaactaac tacagtacaa
    10741 agagttgaaa tggcacatga ttattccgat gattttgcaa tactaaacaa agttttaaaa
    10801 agtgatatag ataatataag gtctacacta gaagaaattg acgagagagc gtttaaagaa
    10861 gcttctaaca aacttttaag agcgagaaaa atatatatac ttggaatgag gagttctttt
    10921 gtagtagctc aatatttagg tttttactta gatataatac ttgacaatgt tcatataatt
    10981 agaatggata tgggagatgc ttttgagcag attgtaagaa taaatgaaga agatgtaata
    11041 gttgctatta gtttccctcg ttattctaag aaatcttatc aaattgtcaa ttatgctaaa
    11101 gaaaaaggag cccatgtaat atcattaaca gatagcttat ttgcgccagt agcatcactt
    11161 gcagataaca ctcttctagt taagagtaat atggcttcat ttgtagattc tctagttcca
    11221 gctcttagta tttcaaatgc ccttgctata tctgttggga tgaaggaaaa agaagatatt
    11281 aaacagcatt ttgatgattt agagcaaatt tggaaaagat attctgtgta tgagtaaagt
    11341 ttgtatatta ataaggctat gaaattgaga atcatagcct tattagtaaa tactcgatta
    11401 tcacttaaaa aataaatttt cgtttaaata atacaaataa atatttcgag actcaaaaca
    11461 taaagatgta ttctgaatac atctttacta cgtcaactaa tttattaaag aaagcatttc
    11521 cattcatgga gagtgctttt tttatttctc taaacaatat catttcctta attaacaatt
    11581 ataaaattaa tttt
//
LOCUS       DAMJZE010000124        11386 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJZE010000124 DAMJZE010000000
VERSION     DAMJZE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.126"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(74..640)
                     /locus_tag="PC541_002740"
     CDS             complement(74..640)
                     /locus_tag="PC541_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2840190.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            806..1327
                     /locus_tag="PC541_002741"
     CDS             806..1327
                     /locus_tag="PC541_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2840191.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2126..4204
                     /locus_tag="PC541_002742"
     CDS             2126..4204
                     /locus_tag="PC541_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2840192.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4204..4983
                     /locus_tag="PC541_002743"
     CDS             4204..4983
                     /locus_tag="PC541_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2840193.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            4976..5785
                     /locus_tag="PC541_002744"
     CDS             4976..5785
                     /locus_tag="PC541_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2840194.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5805..6287
                     /locus_tag="PC541_002745"
     CDS             5805..6287
                     /locus_tag="PC541_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2840195.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6308..6712
                     /locus_tag="PC541_002746"
     CDS             6308..6712
                     /locus_tag="PC541_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2840196.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(6868..8028)
                     /locus_tag="PC541_002747"
     CDS             complement(6868..8028)
                     /locus_tag="PC541_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2840197.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8236..9786
                     /gene="xylB"
                     /locus_tag="PC541_002748"
     CDS             8236..9786
                     /gene="xylB"
                     /locus_tag="PC541_002748"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2840198.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            9882..11219
                     /gene="xylA"
                     /locus_tag="PC541_002749"
     CDS             9882..11219
                     /gene="xylA"
                     /locus_tag="PC541_002749"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2840199.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         4392 a         1308 c         2054 g         3632 t
ORIGIN      
        1 taataaaaat gttatataaa aatgagtata tagcatttta aagagtaata aaccttacat
       61 aataaatgat ttattaatat gaactagaag tatttttact atattgtttt ataaaacctt
      121 taggcatttt acttataaaa tataccacca taatacaaga caaaggtttg tctactatat
      181 tactaagaat ccttggaaag aacgcagcag taaatatctt ctgaccagac tgaactaaaa
      241 aaccaaccaa taaatcagca ccacttccac ttaaaccacc aaacaataaa actgaaatag
      301 gagtaccaat taaaggacaa acaatagata aaattactcc tgtaataata gcttctttat
      361 aaccaaaacc ttttttctta gaaatatatc caacaataag accaaggaca atatttacaa
      421 gggcaaaagg tatatttgta ggacctgata taacacctaa aactaagttt gtacatccac
      481 caataagagc accccaaaaa ggacctaata cgacagcccc aagtacagta ccaactgtat
      541 ctaaaaataa taatggtata tttatcatag atataaccat acctaagaca atattaataa
      601 ctatgcacat tgaagaaaat gtgacttttt tagtatccaa ttataatacc tcctaaaaaa
      661 atatatacat ttatcatagt atagattata aaagattact aattgtatat ttctatatag
      721 aatattggaa atatagaaca aataaataat ataattatca aaatatgtag tatcattaaa
      781 attaagaata actaaggaga ttattatgaa aagaaataga gagattgtag tagtatgtca
      841 ttgtatatta aattgtaatt ctaaagtgga gggattgtca gaatttaagg gagcacaaaa
      901 tatagcgata aatttaataa ataaagggta tggaataatt caactaccat gcccagaaat
      961 gattatgtat ggaataaata ggtggggaca tgtaaaagaa cagtttgaca atttgttttt
     1021 tagagaacaa tgtaggcaaa tgttaatacc tcatgtaagg cagtttgatg aatatttaaa
     1081 aaatgattat gatataaaaa gtatcattgc aatagatggc agtccaagtt gtggttataa
     1141 taagacatgt agttctaaac gttggtttgg tgagttaagt ggttgcaatg aattgatgga
     1201 taaaataaag gatatacaaa tggtggatgg gaaaggtatt tttatagaag agctagaaaa
     1261 gctacttaaa gaatataatt tagagatacc tatagttgga tttgacgagc ttagagaaga
     1321 taagtagaga tataaatatt tattgtctca ttatgaaaca ccattatatt tggcagaaaa
     1381 atatgactga tggacagtac aggaaccatt tttaagtgga gtaattagta cttataaaga
     1441 tgtatccact agctgaaaga aaatgtagaa aggtaataaa tgaattaaga agtagataat
     1501 atatataaag gagtgtctaa aagtgaactt tttagtttat gagacactct ttttttatat
     1561 taaatcaagt atattaaatc aaatatattt taatcatttt agcaatgaaa aataaattta
     1621 tctatatttt gagacaacat cttttttagt tttatttatt tgttgtcgtt tttgacttgg
     1681 tatacaattg tatattttct taaataattt cataaattat ttataattat aaaatccatt
     1741 gttttgaaca atatctaata tattattatc tgtatttata aggtcactat acatattttc
     1801 aagtccgata ttatctacat aataggcaaa tgttattcct gtatgtgttt taaaaaaact
     1861 agatgtatat aggtatcatc acagatatta aaagtataaa taaaagatat taagaacaac
     1921 tttccagcaa tatattagaa aattttacta aaatccggaa agcgattgac aaacaaagtc
     1981 aaataaagta tattattggt aagttagttt aatgtacaaa tgaattatat ataaaaccta
     2041 atttataaat aacgatacta aacgacatta tttaacttaa gttacacaaa gatagaatat
     2101 tataccattg atttaggagg tagccgtgat aaaaatatct gaagactata ttgaaaaaag
     2161 atttgacaat gaactattgc gaatagaagc atggggaaaa aactctttac gtattaggtc
     2221 gtttgtagac caaaattttg ttgatgaaaa ctatgctctt aacgaaaaac ctaagttaaa
     2281 taaagatgat attacaatta ataaaaatga agatggaagt gcaatcatta aaaatggaaa
     2341 gataaaagct gtcttagacc atagagatag aattactttt tataatgata aaaatgaaat
     2401 attattgaaa gaatatattc gattaagagc tgtaaaacat gatgatggtg gagaagatgt
     2461 agggacaatt gagattacca aagactttaa ctctactcta aagttaaaat caagagaata
     2521 tcgaccaagc tatagtggag aatttgaagt aactactaga tttgaatctg agccttctga
     2581 aaagattttt ggaatgggac aatatcaaca taaattttta gatttaaaga atactgtttt
     2641 ggagttagcc caaagaaatt ctcaaatatc agttcctttt tatatttcta gtctaggtta
     2701 tggactttta tggaataatc cgggaattgg aaaagtatct tttgctaaaa atatgacaga
     2761 atggaagatg ttttctacaa attttataga ttattggatt acttgtggag aaagcccaaa
     2821 agagttaaat aaaaattact ctcaagttac tggaactgtt cctatgatgc ctgaaaacct
     2881 tttaggacta tggcaaagta aactaagata tagaacttca gaagaagttt tagatgttgt
     2941 gaaggaatat tctaaaagag gaattaagct ttcatcaatt gcaattgact attttcattg
     3001 gcctaagcaa ggtgaatata aatttgactt agattattgg aaaaatccta aagaattagt
     3061 taaaaaattg aaagaagagt attcagtaga accaatagta tctgtatggc ctacagttca
     3121 aagtgatgca gaaaactaca atgattattt agaaaatggc tatttagtca atgttaatcg
     3181 tggtgttaga atgacaatgc aaattcaagg aaatacagtc tttgtagata tgacaaatga
     3241 aaatgcaaga gaatatgttt gggatagaat agataaaaat tataaacaac ttgggattga
     3301 ctattattgg ttagatgtag ctgaacctgg ctattctgtg tatgattttg ataactatag
     3361 atataaaaaa ggaaatgttt taagttgtgg taatatttat cctattgatt atttaaaaat
     3421 gatttatgat ggtttacata atgacataga gtctgttgta actcttgtaa gaggagcttg
     3481 ggcaggagct caaaagtatg gagctttagt atggtctggt gacattgatt ctagttttga
     3541 agcttttaat aatcaagtaa atacagggtt aaatatggga ctggcaggta taccatggtg
     3601 gactactgat attggaggtt tccatggagg aaatcctaaa gacccagaat ttagagaact
     3661 tatggtaaga tggttccaat atgctacttt ttcaccaatt ttgagaatgc atggagatag
     3721 attgcctcat agtaaaccat tatcaaataa aggtggaggc tcaatggtaa ctggagctcc
     3781 taatgaaata tggtcttatg gtgaagaagt tgaagtaatt cttacaaaat tcataaaaat
     3841 tagagagagc ttaaaaactt atttaaaaaa actgatgaaa gaagcgcatg aagatggaac
     3901 accagttatg agaactttat tttatgaatt tcctgaagat gataaaactt gggaggtgga
     3961 taatacatat atgcttggag atgaaatttt agtagctcca ataatgaatt ataaagatag
     4021 aagtagaaaa gtatatttac caaaaggaca tacatgggaa aatatattta gtggagttag
     4081 ttatgaaggt gggaaaactt atgaagttga atgcccactg gaagaaattc ctattttcct
     4141 aaagcaagat tccagttata attttaaaga actaaaaaat attttgggag aggtgaaatc
     4201 ataatggagc cacaattatg gcaattatta ttaatcgtac tatatggatt ttttataaac
     4261 tatgaaaaaa attcaacaat gtttggaaca tatcaaccag taacagcagg ttttattact
     4321 ggtcttatct taggagatat aaatacaggg ttatatattg gaggtacatt acagctatta
     4381 tcattaggta ttagtaactt tggtggagca tcaatacctg attatcaaac agcaagtata
     4441 gtagccacat ttataactat tacgactaag caagaagcat ctgtaggaat ctctataggt
     4501 attccagttg ctttattgat ggtacaatta gatgttctta gaaatactat tggtatatgg
     4561 ctagttcata aagcagaaga tggagctaaa aaaggaaatt ataaaaatat aacctatatg
     4621 caaatgttag gagtactatt aacagcagct acaacaggta ttccagttgc tctatctgta
     4681 atatttggac cttctttaat taataccatt ttgaaatata cacctgaatg gttaacaggt
     4741 ggtttaacag tagctggagg actacttcca gcagtaggga taggattatt acttagatat
     4801 cttcctgcta aggagtattt tagttactta gtaattggtt ttgttttagc agtatatatg
     4861 aaagtaccat tattaggtgt agctttaatt ggtggagcaa ttgcacttat aatttacaaa
     4921 aagaatttgg aaaatcaaga acaacaatat actgtagtag gaggtatgga cgaagatgag
     4981 tgataaggta tcattaaata aatcaaatga aaaggtgaaa aaaagtgtct tatggcgctg
     5041 gttttttact agttctgttt cttcaaatta tgagaagatg caggccttgg catactgtta
     5101 tgcagtacta ccttttttaa aagtagtata caaagataat ccagaaaagt tacaaagtgc
     5161 tgtattaaat catttacaat tttttaacac taatccttgg gttgcccctt atattttggg
     5221 tattaatgta gcaatggagg agttatcaag tgaagagact gaagaggctg ttgcttcaat
     5281 taaaacaggc ttgatgggac cagtagcagg tttaggagat agtttatttg tcgttattcc
     5341 gtggacaatt tttggagcta tagcagctaa tatggcgata gatggaagtc catttggaat
     5401 cttactgtgg attgtagtga gtgtagcatt gaaattatta agtataccac tatttaatgc
     5461 tggttataca tcaggtacta agctaatatc ttcaattgaa aaaagtttaa agattttaac
     5521 tgagagtaca tctattttag gtttaatggt tgtaggtgca ttaatacctt cagttgtaaa
     5581 agctaatgtt gctttgaact ttcaacaagg tgattttaca atgaaaggtc aagaaatatt
     5641 agatcaaatt atgcctgggt tagttcctgc aattttagtt gtgattgttt attgggctct
     5701 taaaaagaat ataaaaccta tatacttaat tttaggagta atggttgttt caataatttt
     5761 atatgcatta ggtattttaa aataaggaaa ggatgatatt agatatggaa atgaaaggta
     5821 ttaaaaatat tcgtatagat gaccgtttaa tccatggtca agttgcaact atgtggagta
     5881 ataaattggg tgttacaaga ttaatggtag taaatgatgc agtagctaac aattctgttc
     5941 aaaaacaagt tttaagaatg gctacacctg caggtatagc gtcatctata attacagaag
     6001 agactgctat aaaaaatatt actgcaggaa agtatgaagg acaaaatgta ttattaattg
     6061 ttaagtctcc tgtggacctt attccatttg tagaggcagg atttaaaata cataatataa
     6121 atgtaggaaa tatgtcaagt agaaaagata caacagttct aagacctaat atcagtgtta
     6181 ctgaagaaga gaaagaagca tttaaaagac ttttagatga aggaattgaa attacaacta
     6241 taatgacgcc tgatgataag aaaacttatc ttgtagacat cttatagatt taaagaggtg
     6301 tattaaattg aatagtatta ttttgatgag tcatggcaat atggctgaag aagtcttaaa
     6361 ttctgcaaaa atgataatag gggattctat agattatcct actgtaaata tgaaacaaga
     6421 tgatggaata gaaggaacaa tagaaaaatt aaaaaaagct ttagaagtgt acaaggaaag
     6481 ttcagaaata attataatgg cagatttact aggtggaact ccatgcaatg ctgctttatt
     6541 agtagccaat aatgacagtc gaattaaaat tatttcaggg ctaaatttag gtatggttat
     6601 tgaatcagct tttttagata caaaggaatt ggcagcaaaa ttagagaaaa taggtaaaga
     6661 taatatttct atggttgaac aaaatgcaaa tttattatct gatgatgagt aggaagatta
     6721 tattaatatt taaaacgaaa tacttatgtt aatacttaat tgatatacaa tttttaaaaa
     6781 atatagctaa tcttgcataa actatataat aaatctaatt ttagaatgta taatttaaag
     6841 ggttatggct gtatatagtc ataaccctta tatttcatta attaaagtta aattttgtat
     6901 attcaaaaag ctcttgatat ttacagcaga gccaccatat agagatgcct cagaccctaa
     6961 tgatgaaatt tctatattta ttcctttgtt aaaagagcta actaacatat cttttgtaat
     7021 ctgtaatatc tcagggatat cactaattat ttggctattt aaatatatta tttcaggagc
     7081 ataagttgta atagcattat ttatagctat tgttaagtaa ctacaaaact catgaataac
     7141 ttttttggca ttatggttat cttcataata tagttgcttt actatatcag aatctatttt
     7201 aggaatattt tctaaagaag aaagctgttc aaataccttt ttttctgaac aatactgttc
     7261 taaacagcca cgatttccac aaggacatag tttaccgtta ggcatgatga tagtgtgacc
     7321 aatttctcca ctcataccat ttctgccact atataattta ttatttatta taatgcctga
     7381 accaaatcca ctgtgaatac tgagactaag tagtgagtta tgtacagttg aaaaagtatt
     7441 ctcagctaaa gctgttaagt ttgcttcatt ttctatatga ataggaaagt catacttttt
     7501 gctaaggatg ctatataagt caatctcatt aagattgtaa tatggagtaa acaatacttt
     7561 attttcacaa gtaatcccat gtatagccaa agtaagacca attaccttat aaggagtgtc
     7621 tattttggat atgttataac tatttataat ttcatctatc aattgtataa cattgtcttt
     7681 acttacttgt atatctgtta atttcttaga atttataata gttccatcta agtaagatag
     7741 agaagaaaat atatagtcgt atccaatgtc catacttaaa gaaatccctg cacatttatt
     7801 aaatactaat aatatgggtt ttcttccgcc actatgagta ctatttccaa ttcctatttc
     7861 atgaactaga gattcatcaa taagtttttt ggtaatagcg gatatagttg ctttattcaa
     7921 tcctatagta gaagctatac ttgccctaga aataggacca ttttttataa tttgttcaag
     7981 taccaatctt tcattcattt ctcgaatagt gtatttatca gtaaccaatt tgatattcct
     8041 ccttaaaata atattgtaat actttttaca caaaaataaa aggttatttt gcattgacaa
     8101 agataattaa atattttatt attagttcat aagttagttt aatatactaa caaaaataaa
     8161 gcaagtaaaa tatacctaaa atataaaaaa attaggatag gaaaacgata gttatgaagt
     8221 ggcattcaag gagggatgaa gttggagtat gtattaggtg tggatattgg tacatcaggt
     8281 acgaaaacag ttttatttga taaactagga aatactataa aaagttgtac atatgaatat
     8341 cctcttatac aagagaaatc aggttgggca gagcaggatg caaatgattg gtggaaagct
     8401 gtagtcgaat ctataagaga agtagttcaa agttcaaata taagcagtga atgtataaaa
     8461 ggaattggat tatcagggca gatgcatggt ctagttatgc tggacaatga aggtaaaaca
     8521 ttgagaaact ctataatatg gtgtgaccaa cgaacagtaa aagagtgtga ggagataact
     8581 gatttagtag gtgaagaaag actgattgaa ataactgcta atccagcact tacaggattt
     8641 acagcatcaa agatattatg ggttagaaac aatgaaccag atatatataa aaatactaat
     8701 aaaatacttc ttccaaaaga ttatataaga tacaaattga caggtgaata tgctacagag
     8761 gtatcagatg caagtggtat gcagttgctc gatataagaa aaagagattg gagcgatgaa
     8821 gtactagaaa agttaaatat agataaaaat cttttaggta aagtttatga atcacaagag
     8881 attacaggtt atgtgactag agatgtatca agtttgacag ggcttaaaga aggaacaata
     8941 gtggtaggag gagctggaga ccaagcagca ggagctatag gaaatggaat agttaaagat
     9001 ggagtagttt catctactat aggtacatca ggtgtagtat ttgcttatac aaaagagcca
     9061 aaaattgata aagaaggtag agttcatact ttctgtcatg caataccaaa tacatggcat
     9121 gttatgggag ttactcaagg agctggcttg tcattaaaat ggtttaaaga taatttctgt
     9181 caaagtgaag tggaagtttc aaattcttta ggtgaagatg tatatgagat tataaataat
     9241 caagtaagta aagtaccaac aggatgtaat ggattgcttt acttaccata tatgatggga
     9301 gaaagaactc ctcatcttga cccttatgca agaggtgtgt tctttggatt atctccaatc
     9361 cattctaaaa aagaaattgc aaggtcaata atggaaggtg tttcatatag tcttaaagac
     9421 tgtatggata taatagagaa tcttaatata gacgttaatg aagtaagagc ctcaggtgga
     9481 ggtggaaaaa gtaaagtatg gagacagatg caagcagata tgtttaatca agatgtatat
     9541 actataaatt caagtgaagg accagcactt ggagttgcaa ttcttgcatt agtaggagct
     9601 ggaatatatg aaaatataca aaaagcttgt gatgctataa taaaaacttc aactaaatta
     9661 gaaccaataa gtgaaaatgt agatacatac aatatatatc atagattgta taaaagaata
     9721 tacaaatctt tgaaagatga ctttaagtta ttagatgaag tagttaatgc aaaaataaat
     9781 aattaaatta gcttaaaaag aaaaataaag atagaaaata gttttaaatt aagaaaacat
     9841 actacatata ttatatgtgt aaaatattag gaggacatga aatgagtgaa atatttaaag
     9901 gaataggaca gataaaattt gaaggagtaa aatctgacaa tgaattagca tttagatact
     9961 acaatcctga acaagtggta ggtaataaga ctatgaaaga acacttaaga tttgcaatga
    10021 gctattggca tactttatgt ggagagggta atgacccatt tggagtaggc acagtagaga
    10081 gaccttggaa taatataaca gacccaattg agatagcaaa gattaaagtt gatgcaggat
    10141 ttgaatttat gtctaagatg ggaattgagt atttctgttt tcatgacaga gatatagcac
    10201 cagaaggaag agatttagaa gagacaaata agatattaga tgaaatagtt gagtatataa
    10261 aagttaatat ggaaaagaca ggtataaagt tattatgggg aactgccaat atgtttggaa
    10321 atccaagatt tgttcatggt gcatctacaa catgtaatgc agatgtatat gcatatgcag
    10381 cagctcaggt taaaaaagct atggaaataa ctaagtactt aggaggagaa aattttgtat
    10441 tctggggtgg aagagaaggt tatgaaactt tacttaacac taatacagaa cttgaaatgg
    10501 ataactttgc aagattctta caaatggctg tagactatgc taaggaaata ggattcactg
    10561 gacaattctt aatagagcct aagccaaaag agcctactaa acatcaatac gattttgata
    10621 ctgctactgt tttaggattt ttaagaaagt ataatctgga taaatacttc aaaatgaata
    10681 tagaagcaaa ccatgcaaca cttgcaggac atactttcca acatgaatta aatatagcaa
    10741 gaataaacaa tgttttaggt agtatagatg caaatcaagg agacctatta ttaggttggg
    10801 atacagacca atttccaaca aatatatatg atgcaacact tgctatgtat gaagtgttaa
    10861 agcaaggtgg aatagcacca ggagggttta attttgactc taaagtaaga agagcatctt
    10921 ttgaggtaga agacttattc ctagcttata ttgcaggaat ggatacattt gcaaagggat
    10981 tattaatagc tcataaatta ttagaagatg aggtatttga aaactttact aaagaaagat
    11041 atgcaagttt tagtgaaggt ataggtaagg atatagtaga aggtaaggtt ggatttaaag
    11101 agttagaatc ttatgcactg caaatgccag ttataaagaa taagtctgga agacaagaaa
    11161 tgctagaagc tatacttaac agatatatat atgaagtaga tacaatttct aataagtaat
    11221 tgtgtagtaa ttaaacttgg atgtttgaat aaaataacag gatatgttct tgtttttatt
    11281 ctaaaacgga caaagataag tatttgtaaa aattctcata atcatgataa attaaggata
    11341 tagacttaag tataattagt ttatatcctt aaaatttcta taaaaa
//
LOCUS       DAMJZE010000125        11274 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJZE010000125 DAMJZE010000000
VERSION     DAMJZE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.127"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            217..1392
                     /locus_tag="PC541_002750"
     CDS             217..1392
                     /locus_tag="PC541_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2840200.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1565..2962)
                     /gene="asnS"
                     /locus_tag="PC541_002751"
     CDS             complement(1565..2962)
                     /gene="asnS"
                     /locus_tag="PC541_002751"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2840201.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3039..3272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3441..3635)
                     /locus_tag="PC541_002752"
     CDS             complement(3441..3635)
                     /locus_tag="PC541_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2840202.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3908..5581)
                     /locus_tag="PC541_002753"
     CDS             complement(3908..5581)
                     /locus_tag="PC541_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2840203.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5591..8989)
                     /locus_tag="PC541_002754"
     CDS             complement(5591..8989)
                     /locus_tag="PC541_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2840204.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9156..10352)
                     /locus_tag="PC541_002755"
     CDS             complement(9156..10352)
                     /locus_tag="PC541_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2840205.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10373..11194)
                     /locus_tag="PC541_002756"
     CDS             complement(10373..11194)
                     /locus_tag="PC541_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2840206.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3593 a         2065 c         1308 g         4308 t
ORIGIN      
        1 tttattttca gtagtaaact atctgtaaat cttaattata ataaagtatt aatcaataaa
       61 gttactcgac attatctaat aaattttatc tatcacaatt ttagattgtt acacacaata
      121 tattttgata taattaaatc ataaacgaag tattccaaat atctaatgat agaaaggaat
      181 acttattttt atgctttatt tttaatggag gcttatatga acttagaaaa aataaaaaaa
      241 ttttttggat ttcctctaga caaaaaacac aaagacaatg ttgagaaaga actctgcaat
      301 tatctttatc atacgggtaa aatcatttgc ataataatag taatatctca aataattatg
      361 atgatatcaa tctcagctag agaaggaggc ccttttgtca caccaagacg ccaatggtat
      421 ttttatatgt atgtaacact attttgtgta tcttctgtct ttatgtttgt attttcttac
      481 ctttggaata aaaaaaagga ttgctgtacg ttttatataa atttatggat aatttatgtg
      541 acttttttct gcctatgggg ttgtgtaatt actctacttg accagttagg tggaaatgac
      601 ctaagtgtat ttagttatat tatgttagca gctgcagctt tctcagtatt aaaatttgga
      661 gaatcagtaa tagtttttgg aggatgcttt ttgtttctta atttattctt acctgggatt
      721 caaactatac caggcaatga gtttagtaat attgtcaaca cattttttat aacatccctt
      781 gccatttttg tttctacaat gctatataga aatcgtgtat ttacttacta tgataaactt
      841 ttaattagtc aacaatatga agaaatatct catataaata atttacttaa cgacaaagtt
      901 atgacagatg accttactaa aatgaacaac cgtaggtacc ttgaagatat tctacaaaaa
      961 aaattaatca gccatgcaaa tcaagaacct ataactggaa tgatgataga tatagattat
     1021 tttaaacaat ttaatgataa ttatggacat caaaagggag actattgtct acaaaatatt
     1081 gccaaagtaa ttttagattt tacaagttct gaaaatgaac atgctatacg atatggtggt
     1141 gaggaatttt ttatatgtat ttttaactgt aatgaggcag atgctaaact taaatcagaa
     1201 tgtttacgaa aaattataga gtcctgcaat tttgagcgtg atgatgttcc tttaaaatgt
     1261 gtaactgtta gtattggagt ttatacaaag aaaattggaa agacaataac attaaatgag
     1321 tttataagta attgtgataa ggccctatat actgcaaaag aacaaggacg taattgtgtt
     1381 gcattttatt aaaaggttac atactattac ataaacctat aagtattata tcgcaaaata
     1441 aaagtaatca aattatatat ttatagtaca ataaaaataa cttgcatatc tctaaaatag
     1501 ataaatatgc aagtcatttt agtgtttata cttaaagtta tataagtttt attatatata
     1561 attattaaaa ttcagcattt ttaggagttc ttgggaaagg tataacgtct cttatgttag
     1621 acatacctgt catatacata attattcttt caaatccaag accaaatcca gagtgagtag
     1681 cagtaccaaa ctttctaagt tctaaatacc accaataatc ttcttctctt aatcccattt
     1741 cattaattct atctaacaat acatcttctc tttcttctct ttgagaacct cctacgattt
     1801 ctccaactcc aggaactagt aaatccattg ctctaacagt tttaccatct tcattcattc
     1861 tcatatagaa agcttttata tcttttggat aatctgtaac aaacactgga caattatata
     1921 tttgctccgt tatatatctt tcatgctctg tttgaaggtc acatccccac tcaacaggat
     1981 actcaaattc atgcccagat tcaattaata attccactgc tttagtatac gttattctaa
     2041 taaactcaga gtttactatc ttatttaatc tttctaacaa tcctttatct ataaaactat
     2101 taaaaaattc catttcttca ggagcatttt ctaatacata gcttataaca tattttatca
     2161 tatcttctgc aagttccatg ttatcttcaa ggtctgcaaa aactatttct ggctctatca
     2221 tccaaaattc tgaagcatgt cttcctgtat aagagttttc agctctaaat gtaggtccaa
     2281 aagtataaac gtttctaaaa gaaagtgcca ttatctcggc atttaattga ccactaactg
     2341 ttaaattagc ctcttttcca aagaaatctt gagtatagtc tattttgcca tcttcagttt
     2401 ttggaatatc atttaaatcc atagttgtta ttctaaacat ctctcctgca ccttcacagt
     2461 cactacctgt tatgattgga gaatgtgcat atacaaagtt tctttcttgg aagaatttgt
     2521 gtattgcata ggcagctatt gatcttactc taaatacagc tgaaaaagta ttacttcttg
     2581 gtcttaaatg tgctatcgtt cttaaatatt caagcgtatg tctcttcttt tgtagtggat
     2641 acgaactatc tgattcccct tctacaacta tgctttttgc atgtatttcg actggttgtt
     2701 tagccccttc tgtagatact aactctccct ctacaagtat agatgagctt attgctaatt
     2761 ttcctacctc tttaaaattt tctactttct catcaaatac tatctgcata tttttaaaga
     2821 agcttccatc atttagctct ataaatccaa aatttttaga tactctagat gttcttatcc
     2881 atccagctac ttttacagtc ttccctatgt actgttcttt ttctctgtac aattctttta
     2941 ctgttataaa ttccttttgc attttaaatc ctcctagtat aattgttatt aatttctttt
     3001 aaatataatt tcaataaaaa aagcccttca tccctatata aagggacgaa agactattac
     3061 tctcgcggta ccaccctagt tgtcataatg acctcttagt taagcaatgc ttaaagccta
     3121 taacggtgcc ttccgtctaa gtctacttac taaataagct ttcgattaga aactccaaga
     3181 tgttcttcaa tataaacctc gtattgagct ttcaccatcc tcaactcgct agaactacat
     3241 tttatatcta ctcttctctt cacagtattt atcttttatt atttctgcta attaacaaaa
     3301 ttataccaat ttataaattt ttagtcaata aatttatata attgatactg attgttattt
     3361 aaaatataat aattctacct tacataatga taataaatac ttatcttcat tacttcttta
     3421 ttacttcaat ttccaaaatt ttataattta cttccattgt caacccagtc ttttgcttct
     3481 tcgacaccct taattgaagg tattgaaact ttactatgag gttttaattt ttcaacatca
     3541 gcccatgctg ctgtcgactc atttccaact aaaagtgaac ttttaagtct tttattatct
     3601 ttacctttta atgctcttgc ctttttactt tccaaaacta tcacctctat tatttatttt
     3661 gtaatagttt tagcaatttt acaaataaaa ttcataagtc taaattataa ttatttttaa
     3721 attaacataa tatcttttca atacttaatt tatacctatt tatatacaaa atatttactt
     3781 tgtgtattca aaatataaaa ctgtttttta attttcatat ttatttttta tgctattttt
     3841 ctactctatc taaagtattt gatacaatta aactaataat caattataat tttacatagg
     3901 ttcttatcta tagagtattt gctatctgtt gaatcgtatt ttttaaattt aaaatcccat
     3961 atccctgcga tacatttgga tatgtatata tctcaagttt tgtagcaccc aatattaaat
     4021 atgtttttaa aacttgtgta aacaatgata atctcggtac attatcctgt ttttctaagt
     4081 attccatcaa taaggctaaa acacctgtga ctatagaact gctcactcct gttcctgttc
     4141 ctgtattata tgttccattt ttatatgtgg atattatatc tacacctgaa gctactatat
     4201 ctggcttaat tcctcttcct ctaattggtc ctttcgaaga accaatccac atactatcag
     4261 tcttattgtt aaatgtacca actgtaatta catcatcact agcagcatac atagttatag
     4321 tagctactga atcaggctct aaaaaccttg tgtctggagc tatcaaattt ttatttggca
     4381 agtatatgtc atattctcca tttattataa gttctgaagt caatcttaaa atccatactc
     4441 caggttttat atctcttaat cttatctcca aattttcttt tccagaggta atatatggat
     4501 aaataaatct cattgcataa gtagtatttt ctaagttaaa ttttcctcta tatatataaa
     4561 agtctggaga ataccttata tcatgactaa cttcacctga aggagatatt atctgtgctc
     4621 ccactttatc tggaccattc gtatttagtg ttatatcaag agcatagtca tctccatctt
     4681 gtattattac atcttgaact tctcccacac tgctaaatct gccagaataa tgaatatctg
     4741 tattcccttg atttcctgca ccactcacta ctaccacacc agcactactc aatatattaa
     4801 aagtatctag tattgaagta gttgctacag cacttgactt aactccaata gtcaaattta
     4861 ttatcagtgg tttattttct gttcttgcta tgttagtaac atacgttata gctgctaaaa
     4921 aatctgaaac actgtaattt atccttccag cataataagt atctgtatat gattttaatt
     4981 taactactat taagtctgat tctgtagtaa tacctctgta ctgagaattt atccttccat
     5041 ttccaactaa tataccagaa actaaagttc cagtacctat attatcttga ctcaaactcc
     5101 catcatttct atttatggct atatttaact cacttcttgt aaattcactt ccaaaaataa
     5161 aaccttctgg aggtggattt gtatttgctt cttggtccca taaatataaa actttactag
     5221 ttccatcatc atttataaaa tcaggatgca aataatctat cccagaatct ataactgcta
     5281 ataaaattcc tctccctgtt atatcattat atggattttc ataaatataa tcagtttcag
     5341 ctgctgttgt aatggtttct ccattattta cattattagt tatttcaatt agactgctca
     5401 ttggttcaga ttcttcccac caagcaactt gtattatatt atttaaaata gtttcatcaa
     5461 aatctaaagg tacatatatt actgcaagct gactatttaa aaccatatac ctatttattc
     5521 cattttcttg tagggcagat tctatatcac cttgataaat tatacaataa gatttttcca
     5581 taaatccctc ctatcttaaa acatcaaaca ttcctctaac atccaagagc ccatatccag
     5641 aatttgtatt tggatatact gtgtttgaat cttttctagc accagcttgc ataaaagttt
     5701 ttattttttg cacatatgct tggtttggat atctaccgtc aacaaaagta tattgaaaat
     5761 acattgcagc agcaccagct gcatgtgcac ttgctgctgc tgtaccagtt atagttgcat
     5821 acgtgttccc aggataagca gctattatat tgacacctgg tgctactata tctggtttta
     5881 acctgtcttc aatagttggc cctcttgatg aactttgcca taaactacca ttaatggtat
     5941 tgtaggctcc tatggttatt aaatcatctt gaatcgcagg ataatttata gtgtaaaaag
     6001 ggtctacctc tctaaatcta gttccacttt ttaaaagttc tctatttgga aggtataagt
     6061 tatatcttcc tgttataata taaactccta ctaatcgaat cttccataca cctctttttg
     6121 cattctttaa agtaacatta gtaaattgct gtccagaaaa agttgttggg tatatatatg
     6181 ttatagaata ttcagtcccc tctaaatcaa ataaacctgt tactttatta taatttgaaa
     6241 ttccaacact tttactttct tcaccagtgg gagatactat tattacatca gctttatctg
     6301 gtctatttaa ccaaagttct aatgataatt cttcctcgtc ttcatttaac tctaattcaa
     6361 cttcaacaga acctccaaca tatggtatta ttccagatgt atgtgtctgt gtattgccct
     6421 catttcctgc tcctgccgtt atacataacc ctctagtaaa gaacgctttt tcactattac
     6481 tcctattggt taaacctgct aggctactgg tacctaaaga catatttata actaatggtc
     6541 ttccaagttc aaaagctttt ttatatgcat actgagatgc agcaaacaac attgcactat
     6601 tataaaaatc atctatcttg ccgagtttta ttattattag ctcagaatct tctgctatac
     6661 ctgcatattc actatttaca tttccaagcc ctgcacatat cccactcaac atagttcctt
     6721 gaccaacttc atcctgagat agactagggt cattttctgc gatagctcga tttatatctt
     6781 ctcgtgtata ttctgtccca atataaaaac catcaggagg agttccttct tttgtttggt
     6841 cccataaata tactattttt gaagtgccat ctggataaat aaaatctggg tgtagatagt
     6901 ctattcctgt atctgcaatt gatattagag tccctcttcc tgttatatta atattaggat
     6961 tatttttaaa gaaatttacg cccatttctt ctgttgcaac tacacctcca aaagtacctc
     7021 gattaatttc tccaagtaat gtcatctttg tagtcgatac agttcttatg attgaaggaa
     7081 gttgcaaaac tcgttgtatg ctattatagt ctgtattatt tatagatagt acacctaatg
     7141 aatcacttat cttaaaaaac ctttgactca tacctaaccc agctagttct tcttcaaatt
     7201 caggagtatg gactatatct attcctgata gtattcctga ttgtctactt gcatttttac
     7261 ttatataaat ttcattctct aagtcattat gtatttgcat attataacca tctataaact
     7321 tataactatt ttcctcatct ttaaatgact tattaatact tctatatcca atatcctctg
     7381 tttctaaatc ttgatttata ttagatagag tgtacaaatc tatatctgat aaatttaaaa
     7441 aaccaaatcc tgatgaatta tttggataag attgattact taatctcctt gctcccttta
     7501 gaagtaatgc ccttaatttc tgtgaataca aaaataaatc atttccattt acaattcccc
     7561 attccataaa caatgaacaa acccctgtaa catgaggtgt agccatgcta gttccagtta
     7621 atgctccact tgtacctcct ggtaaaaaag atactatatc ttcacctgga gctaataaat
     7681 caggtttaaa aacacctaat tgtgtatctc cttcaccaga aaatatagat actatatctg
     7741 ttcttgaatt aaaactacct actgttataa ctctacttgc tgttccagga actgttacag
     7801 taagctcttg agtaggaatc agaaatctag tatttctatt tagaccttca gaagttggca
     7861 aatatatatt tacattacca gttactatat ctattggttc aaatacaatt ttccataacc
     7921 caggagttat ctgtgtattt gaacttaatt gtagagtaac tcttcttgtg agagaatacg
     7981 gtgctatagg gtaaaaataa ccagttattc tagtttcacc caaagtattc cttatctcac
     8041 ctgaagttag agagattgct tgtgtttgat tatttgatgg gtttaccaaa tgtacactaa
     8101 aatcatcaac aaaatctggc catatattta tgtttaatat acgctctcct tccccaacta
     8161 taaactcaac ttcttctgta atattatttt gaagcctaat ccttttatga cctcctttat
     8221 ctgcattatt tccagctgct acaactatat tgtttttcca aaataaacac atatcatcta
     8281 tatattgttc aaataaagat gttcctctat gtgaaccttc attacttcca taacttatat
     8341 ttaatgtaac aggcattctt aattctaatg ccctatctaa aataaattta attgccctca
     8401 taaactctgt acttctcgaa aaaatatcgg tctgtatatt tccaactcta accactatta
     8461 ttctggcatc acttgcaata gttgcacaaa tacctgcaac atgtgttcca tgtaagctag
     8521 ttgttgaaat aggtatatac atacttccat ctattgcatt atttatatct tcattagtat
     8581 ataatgttcc ttctctaaaa ccttctggtg gatttccttg aatagattgg tcccaataat
     8641 ataatatctt agaccttcca tcactatctc taaacacagg tagagtataa tctattcccg
     8701 aatcaattat accaattata gttccttttc cagttaaacc agttctattt ttaaatcctg
     8761 ttattcctgt acttgaaaaa ctttgcacat cctgagtctg taatataaaa ggcttttcta
     8821 taaattcaat ttctggataa gttaaaagta tatttacatc ttcttcatta cttgatgtta
     8881 ttattgcata tgatgaatta agtatctcta ctgatacacc taattcttct tctaacctca
     8941 atatatctcc attgtatttt acaattaatt cataatttat tataatcacc tcgacttcta
     9001 aaattattat taatgtatat gttttgtgtg agtctaaaat gtattagact atataatttt
     9061 ttaagtttca gttatacaat ttataataga tatattaaaa aaataaataa tgatggtaca
     9121 attttgaata aatcatacca tcatttatat ttactttatc ttgaaaccat taaatttttc
     9181 aatgctttct caagaccttt tacagtcaat ggaaacatat taaattcact ttgtatcatt
     9241 ctttgagttt gataccaata actaattgaa tcccatccat ctctaagttc tggattcatc
     9301 catacaattt tactgtactt tctcttaaac cttttaagcc actctatacc aggtgtataa
     9361 ttatatggac cacgatagtt accaccaata tgaagcaact cagaaggcga catagaagca
     9421 tctccaacaa ttataacttt atagttttta tctatatttt ttaatatcca ttcagtatta
     9481 atagatttac ttaaccaaca ttcaggtgtt gtaaataatc tatcataaat acagttatgg
     9541 aaataatata tctttacatc tttaaaatga tttgatttac ttacagactg aaataatgta
     9601 ttacataaag tgctataccc tctcattgaa ccacctgaat ccataagcaa taaaagcttc
     9661 actgtatttt ttcttggctt ctcaaaaaat aactttaaat atcctgcatt attacaagtc
     9721 tcttcaatag tcttatccaa atcaagctct gtctttggag catctattct agtagaaaat
     9781 tgacgaagtc tctttagtgc aacttgaaat tgacgcatat ttaaaacttc atcatctcta
     9841 aaatctctgt attttctttc tcctgcaact tcaagaacag ctctaccata agtagaattt
     9901 ccacctatac gtataccagt ttgccctttt cctgaatgtc ctaactctga agtgcctcct
     9961 gtgcctatcc aataattacc accattatga ggagaatctt gttcttttat tcgttcctcc
    10021 aatttagctc ttaatttatc aagatttata tttggatttt gttctaatct ttcaaactct
    10081 tctaaatcta tatctggttt tttaagccaa ttcataatct cttctggaat ttcttcttgg
    10141 tgttctatac ctttaaaata ctccaaaaac actgcatcaa acttatcaaa atcactttca
    10201 ctttttatta aaatcatcct gcaaagatag taaaaatttg aaaagtttga atagcacagt
    10261 cctttatcca atgcttcaat taaagtcatc cattcattta aagatacatt taatcctctt
    10321 gacctcaata aataaaaaaa ttcaataaac ataattatcc agctcctatt aactataagt
    10381 gtctttgcat agattcaata tcctcattat tcttaagtag tattcctgca aaaggaactt
    10441 ctttttctat tttttcaata ggcattccac tcaaagttaa tgcttgtatc cagtctatta
    10501 actctgatgt actaggcttt ttttgtatgt cctttaaact tctaatccaa taaaaagtag
    10561 tcattacttg ttccaatagg tgttcttcta ctttatcaaa atgtacttta actatttctt
    10621 ccatcatgtc tctatcagga aattctatat aatgaaatat gcatcttctt aaaaaagcat
    10681 ctggaagttc tttttccgca tttgaagtta taataactat tggtctttgt ttagctctta
    10741 cagtttcttt tgtctcattt atgtaaaact ccattttatc caattcccaa agcaagtcat
    10801 ttggaaattc taaatccgcc ttatctattt catctattaa aagaattact tgttgatttg
    10861 agctaaaggc ctctcctagt ttaccatatt ttatgtactt gctaatatca tctacacctt
    10921 ctcctccaaa ttggctatca taaagtcttt gaacagtatc atatacataa agcccttctt
    10981 gtgcttttgt agttgatttt atattccaaa ttactaaatc tttttttaat tcatttgaaa
    11041 ttgcttgtgc tagcatagtt ttacctgttc caggttcgcc cttaatcaaa agtggctttt
    11101 caagagcaat agcaacatta actgatgcca tcaattctgg tgatactaca taatcactac
    11161 ttcctctaaa gttagatatc tcttttttta tcataaatcc tactcctttt aaacagtatt
    11221 ttctaaatgt atatagactt tattactatt tttgatataa aaataataat acaa
//
LOCUS       DAMJZE010000126        10948 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJZE010000126 DAMJZE010000000
VERSION     DAMJZE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.129"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..1164
                     /locus_tag="PC541_002757"
     CDS             <1..1164
                     /locus_tag="PC541_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2840207.1"
                     /translation="QKYLYPNIYNKNDFNVIIDGKNYGLPNDNMQLNEKKPFLKLKTR
                     IRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSEKGIHRKTDIPNEFSGYIL
                     RIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHNLKPNLEIGYINNLIKLEK
                     TIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGLYTWFYKGRDSIVKQTFSN
                     YSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMADKIKVISESLFEKINSIKT
                     KCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNAQNRKKIMLEIEKLFKKYN
                     YDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYYNLIYTKSEENENE"
     gene            1151..2146
                     /locus_tag="PC541_002758"
     CDS             1151..2146
                     /locus_tag="PC541_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HDF2840208.1"
                     /translation="MKMNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALK
                     FPMKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQK
                     VLENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPK
                     AKDKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKN
                     DYKKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDL
                     ICINIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            2148..2888
                     /gene="cas5b"
                     /locus_tag="PC541_002759"
     CDS             2148..2888
                     /gene="cas5b"
                     /locus_tag="PC541_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2840209.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            2912..5470
                     /locus_tag="PC541_002760"
     CDS             2912..5470
                     /locus_tag="PC541_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2840210.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   5660..8595
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5660..5689
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            8680..9174
                     /locus_tag="PC541_002761"
     CDS             8680..9174
                     /locus_tag="PC541_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2840211.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            9209..10207
                     /gene="cas1b"
                     /locus_tag="PC541_002762"
     CDS             9209..10207
                     /gene="cas1b"
                     /locus_tag="PC541_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2840212.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
     gene            10418..10708
                     /gene="cas2"
                     /locus_tag="PC541_002763"
     CDS             10418..10708
                     /gene="cas2"
                     /locus_tag="PC541_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2840213.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT         4686 a          990 c         1676 g         3596 t
ORIGIN      
        1 caaaaatatt tatatccaaa tatatataat aaaaatgatt tcaatgtaat tattgatggt
       61 aaaaactatg ggttaccaaa cgataatatg caattaaatg aaaaaaagcc atttttgaaa
      121 ttaaaaacaa gaattagaaa ttcaactcct tatttgatta gtgatgatga agttttgtta
      181 cagaaagctt tttttgatta tctaacgaat aaagcagcga atggagaaga agtaatatat
      241 ataagtagcg aaaagggaat tcatagaaaa actgatatac caaatgaatt tagtggttat
      301 atactgagaa taaaaaaggg taaagaatta gaaatacatg attttgatac tgtggtaaat
      361 ttaaacacta aaataaaaag aataaatgta gaatgtataa taccaataga atatgaaaga
      421 cataatttaa aaccaaattt agagatagga tatattaata atcttataaa attggaaaaa
      481 acgattgata ctgtattctt taataaattt atgtgcaata gttattttaa agaaattaaa
      541 aatataaact taagtgattt agttctaagg gatatactta ttagacatag agaaggttta
      601 tatacctggt tttataaagg gagagatagt atagtaaagc aaactttttc aaactattct
      661 atgggtataa ttgaaaattc tataaaaaac aataactaca taagagcaaa ggagcaatat
      721 aatttaagaa tagctataat aaattatttt catggaggaa gtaatatggc agataaaata
      781 aaagttatta gtgaatcttt atttgaaaaa ataaattcta taaaaacgaa atgtatacaa
      841 agtgatgaag aatattattt tgcagttggg caattagcat cgtattttat atctaaaaat
      901 aagtcaaaaa ataaaaatca ttctctgatt aatcctatac taaatgcaca aaatagaaaa
      961 aaaatcatgt tagagataga aaaattattt aaaaaatata attatgatat aagttatgac
     1021 aacaataggc gatttaagaa tctatattct atgattgtag gatatgaggt agaagaaaaa
     1081 aaagttaata gagatttatt aattggtgga tatctatatt acaatttaat atacacaaaa
     1141 agtgaggaga atgaaaatga atagaagagt atatggagta ttaggtataa aatctataat
     1201 gtcaaactgg aatgctgatt tttctggata tccaaagacg acatcaacag gagaagtttt
     1261 tgggagtgat aaagcactta agtttcccat gaaaaaaatg tgggaaaatg aaggtgaaaa
     1321 agttttatat gttaaatcat taaaaataga tgaatctaag aaaggagaaa taaaaattag
     1381 accaaaatca ttaaaagaga gatatgaaga attgtttgat atagaggacc taaaactaga
     1441 aaagaatcaa caaaaagtac tggaaaatct atttaaagct attgatgtta aaaattttgg
     1501 tgcaacattt gcagaagaag gaaataatat aggaattatg ggcgcagtac aaattggaca
     1561 aggatttaat aagtatgaag atattaatgt tgaagaacaa caaatattat ccccatttag
     1621 ggatccaaaa gcaaaagata agaaaaagtc aacaaatgaa gaagaaaaag aagatgcaaa
     1681 ttcaactaca ttaggaaaaa aaatagtaag tgatgaggct cattactttt atccattttc
     1741 aataaatcca aaagcttatg aagtttttgt taatatgggt gtgactgatg gatatactaa
     1801 aaatgattat aaaaaattta aagaagctgc tttagtaagt gcaactgcat tcaatacaaa
     1861 ttcaaagctt ggttgcgaaa atgaatttgc attatttgtt gaaactgatg aagagttata
     1921 cctaccagat ttggcacaat atataacttt tgaaaagaga gaaaaatcaa aagatttaat
     1981 ttgtataaat ataattcatc ttatagcaga attaaaagat gatataaaga gcgttgagat
     2041 atattataac ccatataaaa tagaaataaa gccttttaaa gatgaaatat tacctaagat
     2101 agataaagtt aaatttatga acatatttac aagaaaagag gtataatatg gaggttttga
     2161 aatttaactt aagtggaaaa actgcttttt ttaaaaaacc agatgtaaat agcataattt
     2221 attttacgta tggaaacata cataaaattg cacttatggg tattttagga gcttgtttag
     2281 gacttggtgg atataatcaa cagagtaagg aagatgagta tccagaattc tactctttac
     2341 ttaaggatat aaaaatgtca atagttccaa agcatgaatt tggatatata aataaaaaag
     2401 tccatatatt taataattct gtaggatatg catcaaaaga agaaggtgga aatttaataa
     2461 taaaagaaca gtggcttgaa aatgttagtt gggatatata tatacaatta gaagattcaa
     2521 ctacaagtag agagcttaaa agaagatttt tatctaataa atttgtatat ataccttact
     2581 taggcaaaaa cgatcatata gctaatataa gtgaaataga gataattact gatttttaca
     2641 aaacagaaga tattaacaga gtggacagtt tctttataag agaggatttt gagtttgaaa
     2701 aatcttattt tgatgataat gatgattata aagagccatt taaatatcag gaaaaacttc
     2761 cttatagact gcaccgaatg actaatcaat atattttgga aactatcata tttactaata
     2821 tgtcagttaa attgattaat aaaaaagagg tatatcaagt aaagggttta aatttaatct
     2881 ttatttaaat ttataggttt aggagaatta tatgtacttt aaaaaagttg agttgtttga
     2941 tataagtaat aatataaata attgcaaaaa tatttatgct catataaata aagacaggaa
     3001 gccagaaact ttaaaagaac attcagactt atcatactca tatcttatga aattatgtgt
     3061 aaaaaaagat ttaagctctt gcctagagag aatagagaat aaatttttta aaaaagattc
     3121 ttttttaagt aagaattttt atagagagat tatcttgaat atggtttata tgcatgatat
     3181 aggtaagatt aattcttcat ttcagtttgt aaaaatgcaa aataaaaatt ttaaaaaaat
     3241 ttcacttaga tatactaaac attcttttat ctcttcaatg ctatattttg attactattt
     3301 tgaaaagctt aaaaaatatg attttgaaga aaaaaaaata ttgagtttat ttttgatgat
     3361 aaatacatat acaatatcaa aacatcatgg ggttctgaag tcatttaaag aatttttatt
     3421 tttatttaaa gatgagttta taaacattaa agaaaatttt aaagatttat ttaaaaacaa
     3481 ttataattta gagcttaaat cagttcccaa aactatagaa aaaatggtaa gaggtgtaga
     3541 agcgtattta gatgatagcc atataaagga taacttagct atagacttat atatatattc
     3601 aaaattgatg ttttcaattg ttacatcttg tgatttttat gctacatcag aatataatag
     3661 tggtaattct atagatgatt ttgggacaat tgataattat aataagtatt atgatgtatt
     3721 aaaaaaatct gaaatatata aggggatgaa aaaatatagg gaatacttaa ataacaatag
     3781 tgaatgccca tataaagaga atgatataaa cagattgaga acagaaatga gtatagaagc
     3841 tgaagatgca attacacata atacgagaaa aagaatattt tatttagaag caccaactgg
     3901 aagtggaaaa actgttacat ctataaatct agcactgaaa tctatagagt taaatcaatc
     3961 tttaaaaaag atattttata tatttccatt taacacatta gtagagcaaa caaaagatgt
     4021 atttataaat gaaatattta attctgttaa agacatacaa aaagatgttg tagttataaa
     4081 ttcaataaca ccaatacaaa cagaagataa agaagaagat aataaaaatg ttgaatattg
     4141 tgttactaaa caagaaattg attatgataa atcattgtta aatagacaat ttttacacta
     4201 tcctatagta cttacaacta atataggttt ttttaactat ttgtttggtg tatcaagaga
     4261 agaatgtttt ccattgatac atatgataaa tagtgtaatt atattagatg aaatacaaaa
     4321 ttataaaaat gagatatgga aagaaattat tttgttttta gaaaaatatg cagatatatt
     4381 aaatataaaa tttattataa tgtctgcaac tttacctaga ttaaataaat taggatgtaa
     4441 tgatgataat tttgcatatc tagtaaaaaa cagagaaaaa ttttttaaaa atcacctttt
     4501 taaagataga gtaaacataa gttttgagat gttaaattca gaaattaatg atattgaaga
     4561 aatatcagaa aaggttatag aagaagcaga aatatataat aagatactga tagagtttat
     4621 aaagaaatca tcagcactaa aattttataa aaatattaaa gaaaaattga agtataagtt
     4681 agaagatgtt tttttattaa caggtgatga taataaatta gaaagaaaaa aaataataaa
     4741 taaaattaaa gatatgaact ctgtaatttt ggttgctaca caagttgtag aggctggagt
     4801 agatatagat atggatttgg gatttaaaga tatatctaca attgattctg atgaacaatt
     4861 tttgggaaga ataaatagat cttgtaaaaa attaaattca aaagtatact tttttaattt
     4921 agatgatgca agtaagatat ataagaaaga tgttagaaaa gagagacatt taactcttaa
     4981 tgaggaaagt aatagaaaaa ttattttaga taaagacttt gaaaagtatt atgataaaat
     5041 aatagccaga atagaagaaa ataagcataa acataatgac aagaatatag aggtttttat
     5101 aaaagatatt gtgggaaatt tgaattttac agaggtaaaa aaatggatgg ctttaatacc
     5161 agatttaaaa gagtatacaa tctttttaaa tactatagtt aaagatgaat ctggaaatat
     5221 gattgtagga agtgatgtat ggtatgaata tatatcacta ctaaaaaatg ataatgttga
     5281 gtattctgaa aagagggtga aaatgtctga aattatggaa aaactagatt attttacgta
     5341 taaagttcag aaatttgata attcatttaa tgatttagtt ggagatattt tttatattga
     5401 tgatggaagt aagtatttta cagagggaaa gtttgataga agtaaattta atcaaaatga
     5461 atttctataa tgtatttaga ttatatagag aatatttatc ccaagttaat taattagaat
     5521 tagtttaaaa ctattgaaat atagatattt tatagcttat atattttttc taggattagc
     5581 tgggataaaa aatctgtata ttagtgtttt cagtatattt aaggggatta tatttttaag
     5641 aagtactgat tttaaagtgg ttaaacagta acatgagatg tatttaaatt tttactacat
     5701 aattaaatcg tagtcaatac cgtacaggtt aaacagtaac atgagatgta tttaaatctc
     5761 tgaatagcca atcaactttg ctgcattact cttgttaaac agtaacatga gatgtattta
     5821 aatttacttc tcctgttttg aaatctatat attcatcatg ttaaacagta acatgagatg
     5881 tatttaaata taatattgat acaaatattt tgggtaaatg gacttgttaa acagtaacat
     5941 gagatgtatt taaattcgat atggtaacaa atttataaaa gatatagaat gggttaaaca
     6001 gtaacatgag atgtatttaa ataaagaaaa acgaaattgg tgattttgaa gcaatgtgtt
     6061 aaacagtaac atgagatgta tttaaattag taactacaat gcttttcctt ttgaagatga
     6121 tatgttaaac agtaacatga gatgtattta aattaataat gcacctattt taacagtacc
     6181 tggtctaaag ttaaacagta acatgagatg tatttaaatt caagtctagg agacaacata
     6241 aatagtacag atgggttaaa cagtaacatg agatgtattt aaattagtaa aaccagatgg
     6301 aactgttgta ataccaccaa agttaaacag taacatgaga tgtatttaaa tttaaaaaca
     6361 tcttgaggaa tatctttaac agtaattgtt aaacagtaac atgagatgta tttaaatgca
     6421 ttttcaggtg catgataaat aggtaaatta gatgttaaac agtaacatga gatgtattta
     6481 aattttttgt aattagccag attatcaact cccaaaatag ttaaacagta acatgagatg
     6541 tatttaaatt aaatatcgtc cttcgtagtc aaagcaacac cagaaggtta aacagtaaca
     6601 tgagatgtat ttaaatcgta aacaaaattt atcaagagaa gaatttctaa ggttaaacag
     6661 taacatgaga tgtatttaaa tttatatttg ataagagtat caaatcgact taaaatagtt
     6721 aaacagtaac atgagatgta tttaaattca atcttaatac acagacattt ttaaaaacaa
     6781 aacgttaaac agtaacatga gatgtattta aataaataga caaatattga atagctctgt
     6841 cttcatcaag ttaaacagta acatgagatg tatttaaata tatttacaaa atctctaaca
     6901 tattttttaa cttctgttaa acagtaacat gagatgtatt taaattcttt taaagagtcc
     6961 tcgggagcct cgaaaacttc agttaaacag taacatgaga tgtatttaaa ttactcaaat
     7021 aaatttctaa tttgtttatc tactaacgtt aaacagtaac atgagatgta tttaaattag
     7081 aatttacaac aatataagcc tctttaacca taagttaaac agtaacatga gatgtattta
     7141 aatatgtttt tagtagtaca tttaataaaa ctttttaaag ttaaacagta acatgagatg
     7201 tatttaaatg aaggaaccgt accttgataa atcacgaccg tacccgttaa acagtaacat
     7261 gagatgtatt taaattgtat atttgatgtt gttatttctc ctgtgtataa tgttaaacag
     7321 taacatgaga tgtatttaaa tgaagttcca cccccaccac ccccactact ttgagaagtt
     7381 aaacagtaac atgagatgta tttaaattaa caatagagta aatataataa acaagcatac
     7441 aaagttaaac agtaacatga gatgtattta aatccccttt tgcatggtac tagacacgca
     7501 tatatggtgg ttaaacagta acatgagatg tatttaaata cgcctatatg cggagtaaca
     7561 gatttttgcc tggatggtta aacagtaaca tgagatgtat ttaaatactg ttttgggata
     7621 gactggaatt aaatggtctg tgttaaacag taacatgaga tgtatttaaa tttttggaat
     7681 gaagcatcaa cagataataa agaaaaagtt aaacagtaac atgagatgta tttaaatcaa
     7741 ggaaacaaaa gagaagtgaa aagaaatata atgttaaaca gtaacatgag atgtatttaa
     7801 ataatctttt aaatggaaat taagtaataa aagagagggt taaacagtaa catgagatgt
     7861 atttaaattc aattaaataa gcattaatct tagtaaaatc aaacgttaaa cagtaacatg
     7921 agatgtattt aaattttctt gtgttaattg aacccccatt ttaagctcca gttaaacagt
     7981 aacatgagat gtatttaaat cagaatacag cttctatatc tggatacatt tgtcttggtt
     8041 aaacagtaac atgagatgta tttaaatgtg aaaagtaata caggtataga gtctaaacgc
     8101 tcggttaaac agtaacatga gatgtattta aatcaatgta tcctcttttt tagttttgga
     8161 gcctcttttg ttaaacagta acatgagatg tatttaaatt agttagcaca ttttatttca
     8221 agccttaaaa cattcgttaa acagtaacat gagatgtatt taaattaatt tgcacactta
     8281 acctctaatc ttaaagtatt agttaaacag taacatgaga tgtatttaaa taacacaata
     8341 ttatctttaa tagaattaaa caaatgagtt aaacagtaac atgagatgta tttaaatttt
     8401 tgttggtctt gtgcttcttt taaaagctga ttagttaaac agtaacatga gatgtattta
     8461 aatttaaaaa gttctgttat taaaacatcc aaaccaatcg ttaaacagta acatgagatg
     8521 tatttaaatt ctgtaccatc tagccaaacc gtttttgaag ttaaagttaa acagtaacat
     8581 aagatgtatc taaattactc tatcataaaa ttttaatata ttaaacaaac taatatgtga
     8641 tatacttatg tataaataac aaaataaata ggtgatgtta tgaaaattac aggaactttg
     8701 ataaattact atttccattg taaaagacaa tgctggttgc ttggtaatag aataaatttg
     8761 gaagaaaata gtgaagatgt aaaaataggt agacttcttc atgaactaaa agaagaaaaa
     8821 acaaaatata aagaaattgc aattgaaaat ataaaaatag acaaattaac aagagaatat
     8881 ttagtagagg ttaaaaagtc tgatgctgat atagaagctg ttaagtggca agttctttta
     8941 tatttaaaaa agttaaaaga aaagggaatt gtaagaaaag ggaaaattga atttatagaa
     9001 aaaaataaaa ctaataaaaa aatagtcttt gtagatttga gtgaagtatc tgaagaacag
     9061 cttatatcta tagaaagaga aattgttaat ttaataaata gtgataaagc accatgtcct
     9121 gatttaaaaa aaggatgtaa aaaatgtgcc tattatgaat attgttatat ataaagaggt
     9181 ataagaaaca tataaggagg tgttaatatt gggaaagaca aaatatatta cctcaatggg
     9241 tgaactcaca agaaaagata attctatatg ttttagaaaa aatggaagga atgtatatat
     9301 accagtagaa aatactagtg aaatattctg tctaaatgag gtatctttaa attcaaaatt
     9361 actggatttt ttatcaagag caaatattgt tgttcatttt tttaattact atggtggata
     9421 tagtggaact ttttatccta aagaatattt aataagtgga aaagtaacag tggcacaggt
     9481 aaaaaccttt gaagatagaa gagaagaaat agcaaaatcc atagtcaatg gtataaggat
     9541 taatgcaata aatacattat cgcattacta taagcatgga aattcagaaa tacaaccatt
     9601 attatcacat ctaaaaaaag attctataaa caagttagaa aaatcaaata atataaaaga
     9661 aatattatca gtcgaaggag atatttggca aacattttat acttccttta agtatatttt
     9721 aagagaggaa tttattttta ataaaagagt taagagacca ccagataatc ccttaaacgc
     9781 aatgatatca tttggtaaca gtattttata tgctaaaact gtaactgcta tatataatac
     9841 acatctaaat caaagtgtaa gttttttaca tgaaccaagt gaaagtagat tttctttaag
     9901 tctagattta agtgaaaatt ttaaacatgt tataactttt aaaactatat ttgatttagt
     9961 taataataga aaaataaata ttgaaaaaca ttttgataaa aatttgaact actgtgttct
    10021 aaatgatgaa ggaaagcaaa tttttataaa ttctttagaa gaaagattag aatcagtcaa
    10081 agaacatcca aaattaaaac gaaaagtaag tttaaaaacc tgtataaaat tagatgctta
    10141 caaaatgtta aagtgtataa tggaagaaaa aagttttatt ccatataaca taaaagacgg
    10201 attataattt aaacataaag taagtatttt aatatagaac tttgatatat ataagaatga
    10261 taattatgct attataagta tacataatat attattgaaa taatataagt ttttagaagt
    10321 attttaagat gagaaaggag aaaatactga aaaataatta ttctattata atttgccaag
    10381 gttttgtata tttttcagtt taaatattat attacatatg aaaaagaaaa acaatttaaa
    10441 ttataattat gtatttgtag tctatgatgt aggagaaaaa agagtaagta aagtttttaa
    10501 agtgtgtaaa aaatatctct cgcattttca aaaatcagtt tttagaggtg aaataactcc
    10561 atctaagatt atgagcttaa aacgtgattt agaaaatatt atagataaag ataatgattt
    10621 tgtatgtata ataaagttga tgaattctaa tacatttgaa gaagaaatat tagggaataa
    10681 tagtaattgt gaaaatatga tattataaat ctaattacta tatttgtaaa aaataatagc
    10741 ttattaaaga gtagtaaaat tgggatataa tttatgatag agactgcaag tagatttttg
    10801 tgaaaaggat ttacttgcag tatttttgtt atgtataact gttaacatta gtgtacaaaa
    10861 cattatcaat agcatttttc tataatgaag aaatactatt ataatgggaa aattttatta
    10921 atactaggta ataatatatt tacacaag
//
LOCUS       DAMJZE010000127        10945 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJZE010000127 DAMJZE010000000
VERSION     DAMJZE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.130"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..613)
                     /locus_tag="PC541_002764"
     CDS             complement(<1..613)
                     /locus_tag="PC541_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2840214.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSG"
     gene            complement(685..1038)
                     /locus_tag="PC541_002765"
     CDS             complement(685..1038)
                     /locus_tag="PC541_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2840215.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1116..1319)
                     /locus_tag="PC541_002766"
     CDS             complement(1116..1319)
                     /locus_tag="PC541_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2840216.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1709..1939)
                     /locus_tag="PC541_002767"
     CDS             complement(1709..1939)
                     /locus_tag="PC541_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840217.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1941..2348)
                     /locus_tag="PC541_002768"
     CDS             complement(1941..2348)
                     /locus_tag="PC541_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2840218.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2708..3157)
                     /locus_tag="PC541_002769"
                     /pseudo
     CDS             complement(2708..3157)
                     /locus_tag="PC541_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3171..4097)
                     /locus_tag="PC541_002770"
     CDS             complement(3171..4097)
                     /locus_tag="PC541_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840219.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4222..5004)
                     /locus_tag="PC541_002771"
     CDS             complement(4222..5004)
                     /locus_tag="PC541_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2840220.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5160..5780)
                     /locus_tag="PC541_002772"
     CDS             complement(5160..5780)
                     /locus_tag="PC541_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2840221.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5868..6566)
                     /locus_tag="PC541_002773"
     CDS             complement(5868..6566)
                     /locus_tag="PC541_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2840222.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6667..7482
                     /locus_tag="PC541_002774"
                     /pseudo
     CDS             6667..7482
                     /locus_tag="PC541_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8154..8921
                     /locus_tag="PC541_002775"
     CDS             8154..8921
                     /locus_tag="PC541_002775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2840223.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9249..9926)
                     /locus_tag="PC541_002776"
     CDS             complement(9249..9926)
                     /locus_tag="PC541_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840224.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10076..10393)
                     /locus_tag="PC541_002777"
     CDS             complement(10076..10393)
                     /locus_tag="PC541_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840225.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10415..10753)
                     /locus_tag="PC541_002778"
     CDS             complement(10415..10753)
                     /locus_tag="PC541_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840226.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3619 a         1734 c         1563 g         4029 t
ORIGIN      
        1 ttcctgattg aatatgctta atgtttgatt gaattttttt atatggccaa tcccaccatt
       61 taatttctaa taattttgaa attgtatcat tattaaatct cttttttata atttttgcag
      121 gtactcctcc aacaatagaa tacggtggaa cgtcattagt aactactgct cttgttccaa
      181 taattgcacc atcaccaata tttacacctg acataattat agaatcataa cctatccaca
      241 catcatttcc aattataatg ttacccttat tgtcccaagc gtccgttata tggttgatat
      301 cgagtttcca ttcttcataa aagatgggaa aagtatatgt ggattttgat ttcattgagt
      361 gatttccact agtcattaga aattttgcat tacatgctat tgagcaaaac ttaccaatta
      421 ttaatttatc attgtttata gggtaatgat acaatacatt gtttttctca aaatctcttg
      481 ggtcattata aaagtcatta tatattgtgt aatctccaac ttctatattt tcccttgtta
      541 ttacattttt aagatataca gtttgataat cattacttct tggatatacc ttatttgaac
      601 ttggaatagt catatttaaa tctctccttt aatatatatt atgtttttga ctattccact
      661 actacaatac atctcttcac agaatcactc cttgttaaga atcttcataa gataatggcg
      721 tcctttttta gtgttaagtc ggatattctt tcttagtcca ttctttttag cgtataactg
      781 acaaactgtc attttcttac caatagtgtc aatgctatca tcaaggtcat tttgtattgc
      841 tttttcctta tcccttaatg atatatcatc acgttttccc tttgggggtt tatcagtagg
      901 tacaagtttc caagagtaaa caaattgtgg tttaccaaat gaatatacat acttatatgc
      961 gtatcttcca tcttttctct ggctctctcc agttcttaaa attctatctt tattatctcg
     1021 tcttttttca gttgacatga taagaagctc ctctcaataa ccgaattgag cttgatacgt
     1081 catatgaata ttataacata taaggctcaa tttcattaga taacgtcaag tgtatcaacg
     1141 attttctcaa attgttttct ctttatttga atgcgattgc tattcataat gacccagcca
     1201 gaatcaaggt tttcatcagc acgtttacgt aattttttct ctccaatacg aaagtatttt
     1261 gacgcttctt caatcgtcaa ggtatatttt tcccaaatag gtacatcagt attgttcata
     1321 taatgtatca ctcctttcta tgttcgtagt tcagaagtag atagatggct ttgataagct
     1381 ccattggtat ttcaaccaat cccattctta tggacagaac cgtgtcatac aggtgtatca
     1441 ttatactgtg gtacaggtta cggcaaaaca accgttccaa aggttattca caaatcacta
     1501 tatagtagct ctcttgatta aggtcatggc gtatagtatt tgttggctat ctgtatcaca
     1561 aacgtatcta tctactttat tcagttgtca aagaacagtc ggcattgatt tgcctataaa
     1621 aaatcacaat agcttgtggg cttttataga caaataatta gtgattctcc atgtaccaaa
     1681 tagcttgata catggagaga gggcgagatt aagacaaatt aaatctcaaa atagcagtga
     1741 ttagttttgt tcctagcatt cgctttgttt cttcttctac atgaatatag ggatttccgt
     1801 attcatcaaa taaagttttc tgtgaaagac gaatgatata gtgttcaaaa tgtttaagta
     1861 ttacgtttat tgcatttaca tcgccatgta cagcaagtaa aataattgta aaaggtaata
     1921 cttctttagt aggtttcatt tcaatgttcc tgcatgagtt ctttcaactc ttttagtgca
     1981 tttgttttgt gataatgtat ggtattttct gctaaatcca tcaatgttgc tatgtcagca
     2041 tttgtcatgt tgaaaaagaa tgaaagtaga attatatctt gttttctttt tgtaagtttt
     2101 ccgattgctt gcgccattaa tacatcttca attcgtatgt tgtagtcgta agaaaccata
     2161 cagatgtagt tagaatcata atcatctaaa atactcaatt tacttaattc tgcaagtgac
     2221 aaagattcga gagataccaa ataatcattt ctgcgtttat tttcgtcata aatatctcta
     2281 gcatagttac gcaaaactgt tttacagaaa ctgtcgaatt gatgttcaat tgtcttttca
     2341 tcaaccataa gatttcaccc tctttccatc gcaaggagtt aaataacctt gcttactcct
     2401 atcacggtgg gagggcgtta aatcttcgat aaatttcaaa aaaaagagaa aaaaatacac
     2461 actctgccta tagggcaaaa atgtgtattc tttaaaacgt atttaattta tattggttgt
     2521 aagccaccac catattgatg tatggaaata tgcattggct agcttttcat aaaaatacaa
     2581 agatgcataa gaaattaata gaggggaaaa acttattctg ttattaaaaa gtaggttaat
     2641 tctaataaat gctattgcgg tcgaattaaa ctatatacat ggcaatcaat catgtttcta
     2701 ttctttatta tgaattgtga cagtattctt caatcttaaa gccacatttt tccaatactt
     2761 tacttgactc tctattgtag gaatatacat tagcatgaat tttgttaagg ttgtattttt
     2821 caaaggcaat tttgcatatt tcttttactg catttgttgc tatccctttg cctcaatagt
     2881 tcctagcaag ccagtaacct aattcagcat ttttattaat agaggtatct tcttcaaatg
     2941 ttaggctgat acttccaata gccctttctt gataaaaaat agcatagcta agttgtttaa
     3001 catctgaagt tgccaaacaa aaactaacaa aatctctagc gttttgctcc ctataagggt
     3061 gggggaagtt gtcacgaaga ttctttgcaa tttctttatc attcgcaaac tcaactaaat
     3121 cggtaatata agcataattc cactctataa gttccatttt tcttctcctt ttatgaaaca
     3181 gctacaagaa atctttctaa gccaatacca cttatcaaac agcgttctct tttatttcca
     3241 agcatttgat aagtccagtc aacaaatcca ccgtcaacta tttcaacggt ttcatttcct
     3301 tgtttgaccg ataatttgaa attgatacct ttataataat tattatcaac atcatcatta
     3361 tgaattgata actctatatt aggaaaaata agtttgatag tttcaaccat tctgtcgaaa
     3421 aatccgtcat tatctttgta tccgtttctt ttgcgtagaa taatagagaa acgaacattt
     3481 tctatttcac ttaatatatc tttataatag tttaagtgct tttctaacaa cattttttca
     3541 caagtgtaag accctgtatc tataccagat gaaaccatac aatataagcc aaaatgtgaa
     3601 tataaacctt tacctacaaa attttgtgct cttattaccc tagcagtagt agccatgtgg
     3661 ataggtatgg tattattttc ttcttttctt tttaatttat cagcaataat tattgccaac
     3721 atatttgagg ggtctgcaag tgtttctgtc cctcgtagag cacttactac gttatattga
     3781 tctacggagc caaaaacgct acagctacct aatggggcag agggagaaag cataatcgtc
     3841 ttgatatcca tattgaatgc ttttctcaat aattttgatt ccaaagcatg aatttcttct
     3901 ggatctatac cgctagggat agtaaatcta tttagttggt atgactttag aatatcagtt
     3961 gcagttaaag tatttgcttg tctatcaaac acttctaata gaagtgagtt caaatctgat
     4021 tttgatagag ctgataactt atcaactata ttcttatccc caattttatt tgtaattcta
     4081 cttaaaatat cactcatagt tatcctccat actgttactt tcaaaataat tatagaattg
     4141 ttacaacaac taatctaggt catttgacca aaaatgaaaa aacactgaat atttactatt
     4201 caatattttt ttactttggg tttatttgat taaatcttga aaatactgta atgcaaccct
     4261 ttcagcttca acctttcgga caataggact ttgtgataat tcctctctta actcaagata
     4321 caggttgata gtagcctgca atgcattaac acagctttta tattcatggt tagcaaccgt
     4381 cttttctaat gcttcaatat attggggtgc gtccaattca atttttctaa ctccacgagg
     4441 taatttacct aagagaagct gtattaatgg ggcaagtact gattgtctta aaaaagataa
     4501 aaaatcaatt gtttcaaata gttctctacg tccaatctta gtgcaagcgt aatgaaccca
     4561 aatccaaaat ctatcttcaa tccactgtaa gtctggagtt gggaagtatg cttctttttt
     4621 agaataaatt tctgttatgc agttgttttc ttcatagaga atagctgaat cttcaaccct
     4681 atctagtaaa ccatctaagg ttgtaaactt aaaatcaaca tgaagaacag gggaatcata
     4741 gagggaaatt atcaatcgag gttctccaac gtgttcgcca gtaaaagcag aaactaatgt
     4801 tccaaactgt tccacaataa gtaatctctc ttcgataact tcttcatagg atttatcatc
     4861 tgctacaatc agaaaatcca aatcagaata ttcatctaac gattttgtga tataagaact
     4921 gctaattgca agtcctatta tcctactatc tctttttgaa aattctaata cgttttgtat
     4981 cattttctgt tgtgttttag tcataatata acacctctca ctgtctttta ttagctatca
     5041 atccaacata attatgcata ggagtattat ataatgaaga aataaaaaaa aataggtcac
     5101 ttgacccgaa ataaaaaagc attgaataat tattattcaa tacttttttg aaattatact
     5161 tatgttgtag gaagattctt taatacctct cgctgattaa ttacactaaa tcttttgttt
     5221 tcataagcaa ttagatgttc ttgtacacat tcatttatag cacgattaat gcttctaatt
     5281 ggagcgttta cttcgctagc aagctgtttt ttggtaagca gatttaattc acttctataa
     5341 tagtgtaagg atatacacct cagaagtcgt tctttaacac ttaaagatga taattcttca
     5401 attaattcag catttacaac taatttacta gatatttctc gaatcagaaa ttttgtagct
     5461 tcaaaatctc cccttaacca gtttaaaaaa tcatctctat gaagtttata tacattacaa
     5521 tctgtgaaag cgacaatttc aacgggtttt ttcccatcat taaattgttc catttctcca
     5581 aaaaaactac cacttctata aagatgaatc gttgagatgt ttcctttcac atttaagatg
     5641 aatgcttctg caataccgtc aatcataaga attacatatt cattttcttg ctcagcaaaa
     5701 agaatagtct ctttcttttt atacgttttt ttaacaagct tctttttaat atcttcattc
     5761 ataaaatcaa ttaaatgcat attcatccct ccttaccaaa tctttgatta aattatatca
     5821 tctttcaatt agttaggtaa gatagtaaaa atatatttac atagaaacta ttccattatt
     5881 attttccagt tgtcattttt ctgcaaaaac ttatcactgt cacgactgga gataccagta
     5941 aggaatgtta ggatatcaat cgctaaactt tcagaatcct ttttacggct caaaatgacg
     6001 taagggtcaa gcaatccttt gttttcatca ttactggtta aattgacaat gtttatctca
     6061 tgggctattt ctggtagcgt ttctttccat ttgccgcgtt ctgctttagg gtcaactatg
     6121 accgcctgtc cgccgaatag taccgcatag taaacaagca tattgttact gaataattta
     6181 ccaccaccca atgaaccgat aaacgcagac gctaacgcat tggtaataga accctttacc
     6241 ccttgactag caagggcagg ttttagatag acattctttc cagtgtccag attgtagcca
     6301 agatagatat cctcatgttc gccaagggtt tgtgtcgctc caaaaccaag tccagccaaa
     6361 aaatccgaag tgacatattg aatataatca ttgatgtaac gtttgctgac aggaataaat
     6421 tcgccatgca atcccaacat atccccaaag ggacgcacga gcttgattga taagtcgtcg
     6481 taaaagtctt tgacttcatc acaacgcctt tttagctcgt caagattctt tgtagataca
     6541 cggacaacat agctaagttt atacatagat tctttacagt taaaagtctg tttttaaaaa
     6601 aatgtataaa ttataaatca aaatatagat actatcaatg acccactagg tgcaaaaagc
     6661 attgatatgg ttaaattatc agacgctaaa agctgggcta tccgaatgag tgaagatggg
     6721 tgtgcttata aatccattga caattataag cgctcactga aagcgtcgtt tttcatggca
     6781 attcaagacg actgtattag aaagaatcca tttaattttt agttaagtga tgttttggaa
     6841 gatacaacag aaccaaaaat cgctctaaca ccagaacagg aaatgggctt attatccttt
     6901 atggggagcg ataagactta ttgtaaatat tatgatgatg ttgttatcct cttagaaaca
     6961 ggactacgca tttctgaatt atgcggtttg actttagaat tggatatgaa aaataaagcc
     7021 attatggtag accatcaatt gttgagtgat actaagattg gctattatgt agaagttcca
     7081 aagaccacac agggtaagcg agaaattcca atgactgaaa gagcttatca atcatttgaa
     7141 cgtatcgtgg aacggcgtaa gaacgctaaa gcagaaccaa tcattgttga tggttaccag
     7201 aacttcctat ttcttaaaca agatggatta ccaaaacaag caactcatta tcaaagcatg
     7261 ttaagagcgt tagtcaagaa gtataataag acccatgagg acaagctacc aaagattacg
     7321 ccacatacct ttaggcatac actctgtaca aatatggcta acaaaggaat gagtcctaat
     7381 atattgcaat atattatggg gcataaaaac attactatga cgcttggcta ttatgcacat
     7441 ggttcattta gttcttcgag agctgaaatg gaacggttat aatcctagta gtatgactag
     7501 agtttactac ttatttacta cttttgatgg cattcttata cggttaaatg ccaacttatg
     7561 caaagtatct tccaaaaaga aaatgccgat actccttgta gatacaggat atatcgacat
     7621 ttaccaactt atgaaaagat aatcaaattt aatataagtt tttcaaataa aaagatacca
     7681 aattcttttt cgagaatcta tataacatta cttaagcttg gttggggtat aaacaattga
     7741 gtagctactt ttgtatggtg ttcaacttta gtcaaaactc ttaagtaatg caaatgattt
     7801 agattcatta atattcaatc tcttcataag tagtgaaatt cgagttattt ctgcataatt
     7861 atactatgat ttatagatta attctattag catccagaat aatattgttt atatgagtaa
     7921 aatttaaaga atattgtgaa aattatagga tgttaaaata aatttaggta aaaaagaagt
     7981 gtttgtatat tatagagtac cttttctaaa atatgtggga ttgttttaag aattagaata
     8041 aaaattattt ttaaattagt atttttataa tgtatagtat ttttaatgta taattaaatt
     8101 gtgcagaaaa tagaatttaa agtcctatag aatttctgat tatggaggag aatatgaaaa
     8161 gaaaagttca agtaaaaaat attactattg gagaaggtag acctaaaatt tgtgtaccaa
     8221 ttattgggaa aaataaaaaa gatattataa aagaggctaa agagttaaaa gatgcatgtt
     8281 tggatattat agagtggcga gttgattttt ttgagaatgt agaaaatatt aaagaagtta
     8341 aggaagttct ctatgaatta agaagttata tacatgatat ccctttatta ttcacattta
     8401 gaagtgtagt agaaggggga gaaaaattaa tatcaagaga ttattatact actttgaata
     8461 aagaaatttc taatacaggt ttagtagatt taatagatgt agagttattt atgggagatg
     8521 aggtcataga tgaagtagtt aattttgctc ataaaaaaga agtgaaagta attatatcta
     8581 atcatgattt taataaaact ccaaaaaaag aagagattgt atctcgttta tgtagaatgc
     8641 aggaattggg cgcagattta cccaaaatag ctgttatgcc gcaaaatgaa aaagatgtgt
     8701 tagtgctact tgaagctaca aatgaaatgt ttaaaattta tgcagataga ccaataataa
     8761 caatgtcaat gtcaggaatg ggtgttataa gtagattgtg tggtgaaata tttggttctg
     8821 cattgacttt tggagctgca aaaagtgttt cagcaccagg gcaaatatca tttaaagagt
     8881 taaattcagt acttaatcta ttacataaga gtattaatta aattaattat atagaaatat
     8941 tagacggtat ttttgaagat ataaattaaa tataaaaaac ttttcagata gtaaaattta
     9001 aattgggcaa ttatatctga aaagcaacta tttagtgtgt attttgaaag tgatatagaa
     9061 aatcttataa atcaactatg gcatatttaa aatatgccat taaaacctac tagacattag
     9121 attatagatg gtatactagc atttctatta tttatagata gtaagttttc ataataactg
     9181 ttttatctaa agtagaagat atatttgtat tatgtatatt tgtcattatt tttttgtaat
     9241 tatgtatatt aatggttata tatgtaatat aatattatca ctctattttt tgtatattgt
     9301 acattacaac atttatatct atcatcatca attacagtag aatgtatggg atacttattt
     9361 tcacttaacc aatatttttg catattcttt gtccaagact ttggaaactt caatactttc
     9421 ttgtttatta taacatcctc atcttcaaaa atattaactt ctctttctat ttttattttt
     9481 ctgttgtata tcttattgat agattctttg tcgagtttta tgtctctata catatcaggg
     9541 caatagcaac aaacataatt tctttttttt cgacgtttaa atttcacaac tttctctatg
     9601 ctaatttcta atttttttgt accatctttt ataaatttaa tatcttctat gttctcgctt
     9661 gaaaaaattt ctgtcaatct taaagtatct acagtttctc taattatttg ctttttataa
     9721 gaagagaatc catatccaaa agaaaggttt aatttattag ggatgtagcc atttttatat
     9781 ttaaacatct taattttttc caattcagca ttatctttaa atttcaactt ttcataccta
     9841 aaagttactt tgctaaaaag agtatattct acatcaatat attcttttgc aatcttaaat
     9901 ttatcgctac cttcaatcac aatcatagcc tatctccaat cgttttatat taaaataagg
     9961 ccatagaaca tggtctaata gtactcatta gcactataag acaatgttaa cacaaaaaga
    10021 caaaaaaagg ttatttattt caaataacct ttttttttat ataaaatata aagaattata
    10081 ttttattctt ttttatgatg tctataatct gtgatatcag atttagttct cttgaattta
    10141 agtcttttaa ctcttcattt atatcatttt ctaaatatgt ttttccagtg gaagtaatat
    10201 cttttataag aacatctgca gaaatattta atatatttac aaattttatg aatgttttaa
    10261 gacttggatt ctttttacca ctttcgattt gagttatata ccaaggcgat actccaacca
    10321 tatttgcaaa ttcttttgaa gttaaatttg cttttttccg ttcttcttta agtcttttac
    10381 ctatataatt cattattgtt actatttccc tcacttatat ttctttgtat ttaatgatgc
    10441 actttattaa ctctagcatt gcaatttgtt ctttttcagt taatttatta atcatttcat
    10501 aacattctaa aggtacagag ttatcaacgc cttctgttgt aataatattt gcaaataaca
    10561 attcaagtgg tatgtctaaa gcagtagcaa ttttcattat aatattgata gatgtatttg
    10621 tctcagctct ttcaatctga ccaatatatg ttggatgtaa acctgccttt tctgctagaa
    10681 actcttgact atatcccaat tttgttctat agacttttat tctatctcct attatttttc
    10741 ctatatttgc caattattat ccctccactt tagattatat tcatagttta agttgtttta
    10801 tatagactat ttatactttt ttagtctaaa tatagactat atatgttttt atactgaaat
    10861 aagtgctatt tatttttgta tagtctgtat ctacaataaa caaatatgga taaatcatat
    10921 ttttaattag agaaatattt tcata
//
LOCUS       DAMJZE010000128        10927 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJZE010000128 DAMJZE010000000
VERSION     DAMJZE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.131"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            170..1018
                     /locus_tag="PC541_002779"
     CDS             170..1018
                     /locus_tag="PC541_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2840227.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1082..3004
                     /locus_tag="PC541_002780"
     CDS             1082..3004
                     /locus_tag="PC541_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2840228.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3086..3949
                     /gene="aroE"
                     /locus_tag="PC541_002781"
     CDS             3086..3949
                     /gene="aroE"
                     /locus_tag="PC541_002781"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2840229.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4149..5366
                     /locus_tag="PC541_002782"
     CDS             4149..5366
                     /locus_tag="PC541_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2840230.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5602..7467
                     /locus_tag="PC541_002783"
     CDS             5602..7467
                     /locus_tag="PC541_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2840231.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7645..9009)
                     /locus_tag="PC541_002784"
     CDS             complement(7645..9009)
                     /locus_tag="PC541_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2840232.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9188..10531)
                     /locus_tag="PC541_002785"
     CDS             complement(9188..10531)
                     /locus_tag="PC541_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2840233.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10604..10705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         4049 a         1371 c         1864 g         3643 t
ORIGIN      
        1 tgttattagt aaagtctcgt gatacaataa atttataaaa tctagataaa tagaaatcaa
       61 aaaatacttt tatttttaag gtaaaataat actactagta ggataaataa aaatataaaa
      121 tcaaaaatac tattaattat aaagaaaata aagggggcaa atttaagcta tgaataatca
      181 aaaaaatctt aatattccaa ttacaattag ttcttataca ttgggaacag aagtttcatt
      241 ccgtgataga gttcgtattg caaaagaagc tgggtttgat ggtattggat tgcgtgctga
      301 aaattatatt gatgcaaaaa atgcaggtgt tacagatgaa gaaatgattg caattcttaa
      361 tgaatatgat atgaaagtta cagaagtaga atatataact caatggggaa gagaagaaga
      421 tagaacggaa gctcaaagag aaaaagagca aaatgtttat catatggctc acttatttaa
      481 tgttaaacat ataaattgtg gattattaga gatgatacca gaagaacaaa ttataactgc
      541 attgggagaa ttatgtgaca gagcaggtga acttattata ggtcttgagt ttatgccata
      601 tagtggtgta ccagatttgg ctactgcatg gcgtgttgtg aaaggatgta atcgtgaaaa
      661 tgcaatgctt atattagata cttggcactg ggctagagca aagcagacag cagaggacct
      721 tgcacctgtt ccagcagata agatagtatc aattcaagtg tgtgatgttt tagaaactcc
      781 ttatgaaaaa cttagggatg aatcactaca tgaccgtttg gctccaggtg aaggatatgg
      841 aaacacagag gaatttgtga aaataataaa agaacacaat atatcaccaa gagtcatagg
      901 ggcagaagta atatctgatt ctctaatatc taaagggtta tccactgctg ctgaaactgt
      961 atttaatgca actaaaaaag ttttggataa agcatggtct gaagtatctc caaaataaac
     1021 ttatataata taaaaatata cagagtatta taatataaaa cttgaaagga aggaaaacct
     1081 tatgaagatt agttctatgt ttacacctat aagaattggt tcaatgacag ttccaaatcg
     1141 ttttgtagtt ccccctatgg gaaacaattt tgcaaacaca gatggtactt taagtgaaac
     1201 ttctaaggct tattatcttg agcgtgcttt aggtggtttt ggattaataa ctatagaatc
     1261 tagtgttgta gacaaaaaag caaaaggagg tcctcgtaag ccttgtttat atgatgatag
     1321 cacaatagat agttttaaaa atgtaataga tgcttgtcat gatgcaggtt cgaaggtatc
     1381 tatacaatta caacatgctg gttctgaagg aaatgaaaaa gtagcaggac atccattaaa
     1441 agctgcttct gcaatacctg catcaaatgg tcgtaataca ccactagcaa taacaacaga
     1501 agaaatatat gaacttatag aatcttatgg agatgctgca ttacgtgcac aaaaagctgg
     1561 tgcagatgct gttgaagttc actgcgctca tggatatctg gttagtagtt ttatatcaca
     1621 aagaacaaat aagcgtgtag atgagtttgg tggatgtttt gaaaatagaa tgagattacc
     1681 tcgtcttatc attgaaaata tacgtaaaaa agtaggaaat tctctagcaa tattatgtcg
     1741 tataaacagc actgatgatg ttccaggagg aattgatgta cacgatagtt cagtaatagc
     1801 agcatatcta gaagattgtg gaatagatgg acttcatgtt tcacgtagta tacatataca
     1861 tgatgaatat atgtgggctc ctacgacttt acatgctgga ttcagtgcag aattagttac
     1921 tgaaataaaa aaagctgtta gtataccagt aataacagtt ggacgttata cagagccaca
     1981 gtttgctgag ttaatggtca gacaaggaag atgtgattta gtagcattcg gtagacaaag
     2041 cttggctgac ccagaaatgc ctaataaagc taaaaatgga aaactagacg aaatgatacc
     2101 atgtattgca tgcttacaag gatgtgtacc aaatatgttc caaggcaaac ctatagcctg
     2161 tcttgcaaat cctatattag gacatgaagc agagcttaag ccagctgaaa ttagcaaaga
     2221 agttcttgtt gttggtggag gtgttggagg tatgcttgca gcatgggtat gtgctaaaag
     2281 aggacataat gtaactttag ttgaaaaatc tgaagttctt ggtgggcaaa tgcgtttagc
     2341 tgcatatcct ccagggaaag gagatattac taacttagtt agaagttata tttcaaaatg
     2401 taatcaatat ggtgtaaaaa tatgtactaa tacagaagct acagtagaac ttataaagga
     2461 gaaatcacct gatgtagtta ttatagctac tggagcaact ccacttgtac ttcctatacc
     2521 aggaatcaat gattcaggat taattcatgc tgtagacctt cttgatggta aaaaatcttg
     2581 tgggaaaaaa gtacttgttg ttggaggtgg aatggttgga tgtgaagtgg cagccttcct
     2641 tggagaacaa gaacatgagg ttactgttat tgagcttaga gaagaagttg gtgcagatgt
     2701 aatttcagaa caccgtaaat tccttatgaa tgattttgaa aattataata tacataccat
     2761 tactggtgca aaagtttcta aattttatga tgatggtgtc gactatttac ttacagatgg
     2821 cacagagcat gatttaaaag gttttgattc agtagtactt gctatgggtt ctcgtaatta
     2881 cgacccatta agtgaagtta taaaagaagt tgtaaaagaa acttatattg ttggtgatgc
     2941 agtaaaagct cgtagggcat tagatgcaac aaaagaagct tttgaagtag cattaaatat
     3001 ataaataata aataaatttt tctttgtaaa taactgaata ggttatttgc aaagaaaatg
     3061 ataatattga gaggagatta tagttatgga aatttcaggt agaacaggat tatttgcatt
     3121 aataggtaca ccggttggtc actctaaatc accagtgatg tataattata gttttaagaa
     3181 attagattta gattatagat atttagcctt tgatatcaca gtagataagg taaaagaggc
     3241 acttttagca ataaaaactt ttaatataaa aggagccaat gttactatgc catgtaaatc
     3301 tgctgttaca gaatatatgg atgaactttc tccagcagct cgtataattg gagcatgtaa
     3361 tacaattgta aatgacaatg gaaagttagt tggtcatata acagatggag taggctatgt
     3421 tcgtaattta aaagaaaatg gggtagaagt taaaggtaaa aagattacaa ttatgggtgc
     3481 tggtggtgca gcaactgcta tacaagtaca atgtgcttta gatggagcaa gagaaatatc
     3541 tatctttaat cctaaggatg atttttataa aagagcagag caaacagttg agaatataaa
     3601 aaaagatgta ccagaatgtg ttgttaatct atatgattta gaagatacaa acaaacttta
     3661 tgaggagata caatcaagtg atatattgac aaatgcaact cttattggta tgaagcctta
     3721 tgacaatgaa acaaatataa aagatactag tgttttaaga aaagatttag tagtaacaga
     3781 tgtcgtttat aatcctaaga aaacaaaaat gattgaagat gctgaagcta atggatgtaa
     3841 ggctattggt ggattaggaa tgttattata ccaaggagca gaagctttta atctatatac
     3901 tggattagaa atgcctgtag aagaagttaa tgaattatgc tttaaataga taagagtaat
     3961 atcttaaaca atgaaaaaac atacgtattt caatattttt aataataaat tacaaattgt
     4021 catagtgggt tttgcctcat agtccagtta aatttgaagc tttaactgac tatactttaa
     4081 atccattatg gggatgagtt tattgtacac aaaaatgtta ataaattaac aagaatggga
     4141 gaaaaattat gaacaataaa aaatattatc ctactgccat agcgttgtat ttttcatatt
     4201 ttttacttgg tattggaatt tcaattttag gacaatacaa accagaattt tcatcaatgt
     4261 ggggggctaa aacattatca gatggaacat tggatgtaag tattgtttta gctgttattg
     4321 ctgcactagg attagggaga cttatatctt atccatttgc aggacctatt tcagataagt
     4381 atggaagaaa agtaagtggt cttataggaa actttctaca tgcaattttc tttgttggaa
     4441 ttgtattttc accaaatttt tatatagcat atgtatttgc aataattgga ggggcagcaa
     4501 attcattctt agatacctgt gtaactcctt cttgtatgga gatatttgct agtttaggaa
     4561 caatagcaaa tatgtttact aaatttacaa tagctttagc acaatttttg cttcctttta
     4621 taattggatt tgttgcagca aattcaatat catttaaagt tatttttata gtaacagcga
     4681 ttcttattgt agtagatgcc tttttgatag ctgtgttacc atttccacca gctaataatg
     4741 ttattgataa caaaggcaaa acagttaaat cagagaagat gaaatttaca ccaacaagta
     4801 tagctcttgt ttgtattgga tttacatgta catctacatt tgtattatgg ttaaattgta
     4861 atcaggaatt gggtaaattg tatgggatgg ctgaccctac aaaaattcaa tccttttact
     4921 caatgggagt aatatgtgca gtattaataa cttctttact tattaaaaaa tatattaaac
     4981 caataagaat attagttata tatcctataa tagctttact tatgctatta gttgtatatt
     5041 ttgttcaaac tccaactatt tgtatgttag ggggatttgt aataggttat tcagcagcag
     5101 gaggtgttct tcagttaaca acgtcaactg caaatgaaat gtttccaact aataaaggaa
     5161 aaataacatc tatagttatg attgcatcta gtatagcaaa ctatgttatt ttaaatatag
     5221 caggtattat tacaaaatca gggggagtta atggaccaaa atatgttgta ttgtttaatg
     5281 tggcaataac atttgttgga attttacttg cattgtttgt aaacatgaga tatgaaaaag
     5341 aaaaagtgta tgattatgat gtctaatatt agtataatat atgcatagag aagtctaaat
     5401 ctaggataga tttttattaa aagtaattaa aattaataag gatattattt acagttataa
     5461 gatagggtat ttacataatt aataataagg atattactag aatttcaatt tctagtaata
     5521 tttttttgcg tataaatgta aacttgatga aataagtagt ataatcaata taaattacat
     5581 catgtaaagg aggataattt ggtggagaat gtttttaagc gattgcagga atttaatggt
     5641 tatgatggat acaaagaatc atttgaaatg aattacctat gtatatatga aagtataccg
     5701 ttaagagaac aagtagagct tgcaaataat ctagtagatg aaattttaaa tatgtataaa
     5761 agtgaatcta atgaaattta tttattggag ggtagtaatt ctaagtcttt aatttgttat
     5821 ttcgaaattt ttatgaaaaa gataaatact ttggtaaaag aaatgattat tgatgagaaa
     5881 tggttatata aattaactaa agaattaata tataagagta aaaaagttga gtatgtaaaa
     5941 ctagggttag ttttaagtga gaaatatcta aatgtagaaa acttaagaga agttgtagat
     6001 acattttcta aatctggaga atacgtattt tatctgagta atactattaa aaaacttgaa
     6061 ttttacaata catatttatt taatttatct aaaaaggcta caggttcaat aaaagtattt
     6121 gctattgtta atatggaaaa tttagattct aaaataaatt cctatctaat tgaagatggt
     6181 tataaggata caaagtacga acgattattg atgaactata ttatttcaat tgtagatttg
     6241 aatgaatatc ttgaaaaacg agatttagat aaagaaaaga taaataattt agcacgtctt
     6301 atatgcaatt acttgctaag tgtagaattt aaatatatag gtaataaatt agaattagtg
     6361 aatcgatttt tacctactgt agtaaattat gggactaact ttgaaagttt atattcaatt
     6421 ttcctaatag ctataaacgt cttaaaagat gaaaatatag agtgcaataa gattgaattt
     6481 gaaaaagaaa taaatggtat cttgctcagt gaaaaatgga aaaatatata ttttgaagca
     6541 ttaagagatg ctagtggaaa aacagaagat attataaaaa tgtcagaaat ttatgatgta
     6601 aacttatctt ttgatgactt gttgccatat ctaaatagag atattagaga ttttgaggta
     6661 tattggcata tatctaaaaa gggaacgaca agttctagat taaaattatt aaattttttt
     6721 gaagagacgt ttaaaataga tgatttaatt ggaaaaatga aagatataga aaaagataaa
     6781 ttgactcaag aatattatga tgatatgtta ttttttattg tgcttaaagg aagtaaatca
     6841 ctatatcctg aaggaaagaa tatatcttta aaaggaatat ttggaaatat aaatgaagta
     6901 agaaaagaat ccataaatat cttaaaaaga tacagagaga aactatctct tgaagaatta
     6961 aaaattgtta aagaagcgta tgaaaaagaa aaaaatgtta tattgaagga tgaattaaga
     7021 agagtattat atgaaagtaa taatttaaaa aaagaatttg taaatattga aaaaataaaa
     7081 gtagatgaac atggtaagga tatctatttg acttcaatag ctgttgctgg ttcaaggttt
     7141 agaaatagag aatatcttga aaaagaattg gagaaaagta agatatacta tttaactaga
     7201 gagaaagata atctatatga tgaaaaagct attaaaatag ttggtgaaac tggatatgtg
     7261 ataggctatg tcccaagaaa agaaaactat attttgagca atttattaga tggaggaaaa
     7321 ctgctgtatt gtagagtaac tgaatataat ttatatgaag attgtatcta cgcaaatgtg
     7381 tacttgagtt ataaagatgt aattgaaaca gttgaaaact ctttaaagat ggttttagat
     7441 aagagtagaa taaaacttat aaactaagac gtttatatat tgcttagatg atgtaatcct
     7501 aactgattgg ttattttttc tggaattatg gtgtatttta tgagcaatgc taatgaatat
     7561 aaaataaaaa aacaattgtt ttgtatatat gatgtatata aatatattta tatattcata
     7621 tttacaaaac aattgaaatt tatgttattt tataatttta aatttagatg gaattttaac
     7681 ttctgcttta tcttttattt tagaagcttg ttttttaact ttatttaata attcagattc
     7741 atctacaact aatacttttc tttccttcat caaccattta ccatcacaaa tagtagagtc
     7801 tacattttca ctagtcatag aataaactat atttccaatt ggattatgca ttggtaaaca
     7861 atgaattgta tctggattta gaataatcat atcagctttt tttcctactt caagactacc
     7921 aatttctttt tctagtaaag cacattttgc tccatttata gtagccattt ctaagacttc
     7981 ttcagctggt acgacagttg ggtttaaagt tcttccttta tgtattagtg atgtaagata
     8041 catgtccctc ataaggtcca ttctattatt tgaaggagct ccatcagtac caattgaaac
     8101 aggtattcct ttttctaaca tttcaggaat tgatgcaaac cctagaacca ctttcatagc
     8161 tgaacctggg tcatgagaaa cttttacatc atgtaatcta aataaatcaa tttccttatg
     8221 agtaagccaa actgtatgag cagctaaaag atttggtcct aatacaccta gtttatcaag
     8281 gtattcaact gttccaactc cattgttttg ctttacaaat gaaatttcat cagctatttc
     8341 agcaatatgc atatgtatac ctgtattgta tttatcagct agttctttag ttctaacaag
     8401 taattcttca gttgtattaa aaatagttct taatgcaaac catattttaa ttctctcatt
     8461 agatgtatta ttaaacttat ggaataagtc taattgagtg ttgatttcct catcagtagt
     8521 tttttgccat gcctcaggaa gaccatctcc tgtatccata actgatttag ctaaacaggc
     8581 tctaagtcca gttttatcaa cagcttcaac catagcatct acatattgac caccagattc
     8641 taaaaatgta gtgacaccag attttatcaa ttcaacacaa catgcagtag atgaaatcaa
     8701 ggaatcttca taattgaagc tactttcgta tggccaaatt ctttctctaa gccaagttaa
     8761 aagtacaaca tcatctgcaa gaccacgtcc taattgttga gataagtgaa catgagtatt
     8821 gactagccct ggcattagta tttttccttt agcatcatat atctctgcat ctttatctac
     8881 aatattaata tcaaacttac caacctcttt tataatatta ttttcaataa gtatgcttcc
     8941 attttcatat acctctctat ctgcattcat agtaattaga tatgcatttt ttataagtaa
     9001 ttgtgacata aacaccactc cttttctata tatttttata tctatatatt atgataataa
     9061 attttctata caaagttaat agcaaaatca atttacatat aaagtgattt tgctatttat
     9121 aaatttaaaa taaataagat tttattctat ataatttaaa atattgacct agaactattt
     9181 aaaaatatta acttagaact aagtttacaa ctgcaagtaa acctataatc caaagtgtaa
     9241 tactgatttt atcagtttct ttagctacta catgcattat tatatatgaa ataaatccaa
     9301 agcataaacc aatacttata ctataagtta aaggcatcaa taaaactgta acaaaagcag
     9361 gtacagctat tttgctatca gcaaaatcaa tctgttttat gctttcaaac ataaacacac
     9421 caaccattac aagtgcagga gctgcagcgt aagtaggaac tataccaagt aatggtgtaa
     9481 atataagtgt agctaagaat aatgctgctg ttactattga agcaagtcct gttcttgcac
     9541 ctgctgcaat accagaagca gattctccaa atgttgtaac agtactagtt cctaagaatg
     9601 agcctattaa agtagaagaa acatctgcat ataacattct tcctaaatct tttatttcac
     9661 cattctcatc ttcaagaccc atttgtttac aacaagtaat aagaaatgct aatgaatcaa
     9721 acatgtctat aaacatgaaa gagaatatag gtccaattaa tgagaatttc aatgcaccta
     9781 caatatctaa tttaaaagca actggtgcta tactaggagg tgttgagatt attgctgttg
     9841 gcaactccac aaatccaaat ataacagata ttattgttgc aactactata cttatcaata
     9901 ttccaccttt aactttttta tattctagta ttgccattaa gataagagca aaaagggcta
     9961 ttaaaactgt tggtgtaaaa tctgcaagtg aaactatagt atctggatta gctattataa
    10021 cacccatatt ttttaaacca acaaaagcaa ggaaaagacc tatacctgct gaagatgcta
    10081 ctgccaattc tctaggaatt gcgtctgcaa ttgtctttcg aagacctgtt actgcgagtg
    10141 taaagaagac acatccagat agaaaaacta ccccaagacc tgtttgccat gggattcctg
    10201 cacctataac caaagagtat gtgaaaaatg catttaatcc cataccagga gctagagcaa
    10261 atggtgcatt tcctaaaatc gcaaatataa gtgtaccaat ggctgtagct agtatggtaa
    10321 ctgttataag ggcggcttta tccattccag cttcagataa tattgctgga tttacgaaaa
    10381 ctatgtatgc cattgttaag aatgtagtaa ctccacctat tacttctgtt ttaaggctag
    10441 agccacgttc gctaaattta aacattttgt ctaaaaagct taatgatgaa ttatttccac
    10501 ctggttgtac attgttagtg tttggtacca tttttacctc caaaaaactt taagatataa
    10561 acaaaaaaat atatattcat atagatagaa tagaacatat attcagagtt gacattctat
    10621 tcatagaaaa cccatttaag gtgagtttgt agaaactatt agaccgtatt actaatatta
    10681 tatgaacatt ttctatttgt ttttctgttg tattatatcg taatattgta ttatttacaa
    10741 taaatataat aaaattattc gtttttttgt cgattttata aaaaattaag gttatttatt
    10801 gaaaaaaatt actttttcca ataattgaat atgtatgagt ataatgtcaa ttattctaac
    10861 ttgttttata ctattaatgg aagtttatta taaatgagtt tgtgatataa ttataaaagt
    10921 actgaat
//
LOCUS       DAMJZE010000129        10836 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJZE010000129 DAMJZE010000000
VERSION     DAMJZE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.132"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(584..1318)
                     /locus_tag="PC541_002786"
     CDS             complement(584..1318)
                     /locus_tag="PC541_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2840234.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2329..2967)
                     /locus_tag="PC541_002787"
     CDS             complement(2329..2967)
                     /locus_tag="PC541_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2840235.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2970..3827)
                     /locus_tag="PC541_002788"
     CDS             complement(2970..3827)
                     /locus_tag="PC541_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840236.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3829..4203)
                     /locus_tag="PC541_002789"
     CDS             complement(3829..4203)
                     /locus_tag="PC541_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2840237.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5365..6555)
                     /locus_tag="PC541_002790"
     CDS             complement(5365..6555)
                     /locus_tag="PC541_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2840238.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6571..8526)
                     /locus_tag="PC541_002791"
     CDS             complement(6571..8526)
                     /locus_tag="PC541_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2840239.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8543..10609)
                     /locus_tag="PC541_002792"
     CDS             complement(8543..10609)
                     /locus_tag="PC541_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2840240.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGEIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
BASE COUNT         3674 a         1887 c         1239 g         4036 t
ORIGIN      
        1 atggtttaaa taatcttctt tactttgtat tatgatacat gctttaaata tttttacgca
       61 ctttatatta taatacatgc tttaaataat cttattcgct ttgtattata acacattatt
      121 taaatatttt tactcactct gcattatagt acattattta aatatttttc ttcgctttgc
      181 attatgatac attatttaaa tatttttact caccttgtat tatggtacat tatctaaata
      241 tttttcttca ctctgtatta tgatatataa cttaaatatt ttacctcact ctacaatatt
      301 atatataatt taaatatttt tcttcacttt gtatactaaa gtttatttaa agtattttct
      361 ttcccattat actatgctgt taaatatttt tcaactgaag tattgtagaa ccttaaaatt
      421 actataaaaa actatattct aaaatacttg taatactacg tatcaccctt aacacttaag
      481 tataatttta aagcaaaatt cttattactt atatttaatt ttgattctac atctttagat
      541 ataattttaa gtaatttaag aaattattgt aaatcatata gtactactca ataattaggt
      601 ttttcaatgt tccaattatg tttctttgat ataatccacc atgactaaaa actatcgttt
      661 ctatatcata attacttata tttttaatag catcttttaa ctcttgtttg ttgctgtgca
      721 ttacatcaac aggcttaagt tctccatttt ctacctgtaa caaatctcca gcaataagta
      781 ctttagactc ttttaaataa agacatatgt gacctctagt gtgacctcct gtatgtataa
      841 cttctatttc ctcacctaga ttcaattttt catggttatc taatgacaca tctactttag
      901 tatatgatga tttaaatcca agtcccataa catttagcat actaagcatc ttatcatcaa
      961 ttttatctac catttgttcc aacatataaa gtttaaaagg agtttcttct cctgttatat
     1021 agctgatttc acttttgtat gcatatactt tgatattatt cttagatttt tctcttagat
     1081 aatttatgtt tcctatatgg tctatatcat gatgagtaat tattatattt tttaacttat
     1141 caaagctcgt gttactttta ttaagagcat caattattaa atcaccttga cccggaagac
     1201 ctgtgtcaat aagtgttgca tcatcttctg tgtagattac aggaatatac ataactttat
     1261 cttcaccgaa tacatttgat gaaattttta aaacatctaa tccctttgct aaattcataa
     1321 tttttctcct tataattact taattagagg tcaatttaat taaagaaatt gagaaaatgt
     1381 agattttcct ctagttatat tttattaatt aaaaattatt aatacctagt atatcactta
     1441 agttgaagga ttactcgact tgattttttt gttattttat ggtatagtaa ttaatataga
     1501 agtaagtaat tgcaagcttt gcaattactt ttattttttt ctaataacta ttttattttt
     1561 gataattcgt ataaaattaa aagtaaagta atatggaaaa tatgaatata gaaaaacttt
     1621 aataagaaaa taaattatga ataaataaaa gaaacgcaat actgtatata gatgattatt
     1681 tagaaaaata aaatctcttt taataaaaat aagcttcaaa ataaactttt agttcataaa
     1741 gcttaggagg aactaaattg agtgcaagtg tatctactgt tcaaaataaa tagttcataa
     1801 agcactcttt tttatattaa atcaattata ttttcgtcat ttcagcaatg aaaaagaaac
     1861 ttatctctat tttgagacag cttcttttta ttaacttcct actttttatt agttttctaa
     1921 aattgaaaag ctttatttat aggattcctt agatttatat tttgacgata taatttttta
     1981 aaaacatctt aaaagatggt atacaataca cacaatcata ctcataattg aaaaataatc
     2041 aatggaaaat aaaattactg gatattatct attaaaaaat acaaattgat tcacaagaaa
     2101 catataatct ataatattat gatgttactc tctcgcacca agtaacttta ttttcaagta
     2161 accttttaaa ttcataccat ctaacttcac tcaataaaaa tggcaatata aatatgtact
     2221 ctatttttta cttttaaata tctgacaagc tttatatact attggaagta aaatacaaag
     2281 taaaacagcc caatctggta aatgtggcat ataaaagtcc cccttacctt atacaagctc
     2341 cttattacta aaaattttaa tagaaataaa cattgaagct aacagtataa ttaaacttaa
     2401 aaggaacaat acaatattca ctatcatgga tggatactga tatataaatg gaatttcaaa
     2461 attgctgtga gctatagtta atggtaatac aaatgatgaa gccatgatta tcaccacgaa
     2521 aaagaatttt gttgtttcat atcctaattt atattgaata ggcatataaa ctccataaat
     2581 aatagaactt attaataaaa caatcaaaat atatgttaag tcaaaagttc ctatttgtgg
     2641 aataagtaaa ttttcaatcc agtaaactaa tgtacaatag ataaatacca ttgcaaataa
     2701 aacatactta gattgaacta agccacgtct aggataagga gttgaacaaa gtaatacaga
     2761 tgcttttgaa tattgactct cctttaataa tagatactgg cacagcataa attctgaaaa
     2821 tataatggat agtacaaatc ccaatatttg tgcaaatgga ggtaaacgcc acaatgtaaa
     2881 taatgggatt cctattgcaa taaacaatat caacaataca tattttttta caatcagaat
     2941 atctttttta actaaatgaa tcaacataat tactctctcc tttcaatata tgcaagcata
     3001 atatcttcaa ttgtaggacg ctctattaaa atatctggca tatttttttg cacttcatta
     3061 atttttgagg taatccctat aaaaccataa cctgtaattt ctaaatttaa aaataatttt
     3121 ttgatttcat catttagaaa ttgaatatcc ccttttactt ttctatgttc gtccaataga
     3181 gtatcttttt cacgctctaa tataatacgt ccattgtcaa tcataacaat catatctgct
     3241 attttgtcta aatcagatgt tatatgagtg gaaaagaaaa caccacgacc acattttttc
     3301 atatatcctg ttagtatgtc aagtatctga ctacggatta gtgggtctaa accacttgta
     3361 ggttcatcca taattaaaaa ctcagcttta tgcgataatg ctaatgctaa tgcaaatttc
     3421 attttcattc cttttgaaag tgtatcaatc ttttgttttg ggttaagatt aaaacgcttc
     3481 atattttgtt gataatcttc ttcacaccaa ttatcatatg aaggtgcaat aatacttttc
     3541 atttctgcca ttgacaactc ctcatagaaa tatgaatcat caaatacaac tccaattcga
     3601 ttttttaatt ccttctcatt tccatcgaaa tctttaccaa agatttttat ttttccacta
     3661 tcacgcttta cgacatccaa tatcgaacgg attgttgttg tttttcctgc tccattaaca
     3721 cctataaatc cagttataca atcttctggt aatgcaaagc ttatattaga taatgaaaaa
     3781 tcggtataag tcttatttaa gttataaact tctaaaatat tttccatatt aatcctcctg
     3841 atatagaata tccatcattt cttgtaactc tatctgtgta attcctagtg cttttgcatc
     3901 cttaatcata tctgccattt tttgttcaac tatacgtctc tttgagtctc ttagtatttc
     3961 taaatttcca gttgaaataa atgttcctat tccaacttgg cgtgtaagaa agccttcatt
     4021 ttcaagttct tcatacactc gtctaatagt caaaatacta acttttaaat cattagcaaa
     4081 atttcttatg gatggcattg catccccttc tttcagttct tcagtaaata ttgcttcttt
     4141 aatttgctct ttaatttgtt ggtataatgg cttatctgat gtgttagaaa tgagcacttt
     4201 cattttttat cacccccttt ttatcatttt cagtgtgatg ttgttatata cacactatac
     4261 acttagagca cttttttgtc aatataaaag atgtaaaata ttactatgac aaaataaata
     4321 tgaatgaaat attagagatt gttgatagct ataaacactt tttaaaacca cataagatag
     4381 aatgtattaa atatttatta aaagtaagaa ttgaatatct aaaaaaaaga ggaatcctgt
     4441 atgaatgatt tgtttgatga taaattaagt tataaactat ttataaaaaa cataaaggaa
     4501 taaatccatt tctaattaca aacatactaa aaaatttaga tagtaataaa tagatagtaa
     4561 taaatagata gtaataaatg ggatgaattt ataagaattt ggatagagga aagagttatt
     4621 caagagtgta gactagaggt aaactctata ttaaaaaaat ttaaatttgg atattatgat
     4681 gcatggaata tttgcagaag taaccttgct ataagtatag aagatttttt tgattacaaa
     4741 agataatact ctaaaatgtg aggatttcaa cattcaatac tgtactttaa ataatatcac
     4801 tgcaaaatac catccacctt ttaccataat tgaataccct aattatttta aaaaagaaaa
     4861 cattagaaac agaaaacctt ggatatataa aataaaaaat actaacttct aaaacacgga
     4921 tactctttga taaataaaac aaaaaataat tttaataatt aaaattggct atctcaaact
     4981 agagataagt ctctttctca cttttaaaac gttgaaaata tattcgattt cgtacaaaaa
     5041 agagtgcctt agaactaaaa agttcacttt gtggcactcc cttttttctc ctctttttaa
     5101 ttttttataa aaattcttca aaaatattat taaataccct atattataca taaatcaaat
     5161 taaagttgca ttttagcaaa taaaaacaaa ttataactat tagcatagca aataaaaaat
     5221 ctgctctctt ataaaatcaa attataatta taaaaagaaa atattcttat aacaaattag
     5281 ctaatataaa attattttat aaattaatac tactttttag cttctcctat aaattaatac
     5341 cactttttta actttgaaac ctatctacaa cattggtacc ataagaccag ctaccacaat
     5401 agaaatcata caagccatac caccaactac cagcttaggc ataatcttag acatcaaata
     5461 tgctctttct tcttccgttt ctccaacagc attacacact tcatttgaaa ttaaatatgt
     5521 agttgggaat cccaacatct gacaaaaccc tactccaaaa gcaagaggtt tagaaccgat
     5581 aattttccaa cctggtaaca ccttcatcat aaggaatata ccaatgatag aagctgcaaa
     5641 catacccaca attggtacaa aaagactaat taaatctgtc aaagaaactt ttgctaaaga
     5701 aggaataaca gctgcaaaca caaccatgtt gataaatccc ttgatttttc ctttttccaa
     5761 aatgtctttt ggcacaatac caatacgtgt tacaataaca ccaacaacaa gacatacaat
     5821 agaataattt acatgaataa tctctgaaag ttcataccct agataagaga ataatgctat
     5881 aaaaaatata caaacatttg tagaataata tttatcgtat ttttcacaga aagcttgttt
     5941 tacttttgtg cctgtattct ctgcttcttt cttatcagta tcaacatttg ctaaatcaat
     6001 gccttttgat tttgcttcat gatattctac taaaagacgg tttgcctctt gtaacgctgc
     6061 tcttgaagct attggtgtac caataaactt ctgaattgca aatacaactg ttgctgtagc
     6121 tgctgcaagt gtcagtcctt tttctagtgc tgcttgtgtc ataatagtag tagcaggtaa
     6181 agctccattt ataactggta tgcttgtcaa tactgttgat tttccaaaca aaggaataca
     6241 taatgagaca cagactataa ctgctacaat tccaagccat gctgtcaata ctgttctcca
     6301 ttcgtctata aattgtttaa cattcaacat tgttcccata ctaaacataa tcattggaac
     6361 actccaacta gctgctgcgg tcatacctgc tttttcaata atatcagctg gtattgcttt
     6421 tgtgacaaat agaatcaaaa ataaaagtag tgtaacaaat acagctgata cttttgcttt
     6481 tgttttgctt gcaattaaat ccccaatagc aaataaaact aatacgacac taaaccataa
     6541 taacatatca ctcatagaat aaccccctta ttataaatat ctttcaaacc aatctacaat
     6601 agcatttgct cttgcaattc tattagatgg tcttccattt ctagacaatc catgtgtatc
     6661 tccgtggaac aaatacattt ctgtatctac accaagctgt tttaatgctg taaacatttg
     6721 gaaagtatcc cccataaaac atctataatc cctatcacta tgtaagaaca atgttggtgt
     6781 cttaacattt ttcacatatt taataggtga atgattccaa agaacctcct catgattcca
     6841 aggcgtctta tctccacaag tctgcttcat aattctatcc ccaaagtcaa taattaaaga
     6901 agctgtaatc aagttactaa tacctctttg agaaactgct gctttgaaac ggtctgtatg
     6961 accaattatc cagtttgtca tataaccacc gtaacttccc cctgcaaccc ctaagcggtc
     7021 tgcatcaatc ccaggatatt tttcaataca agtatctaca aattccataa gatgctcata
     7081 gtcaacatac ccccatcttt ctaccatttc taagaatttt tcaccataac cgtcaccacc
     7141 atgagggtta caataaatca catacattcc ctttgatgtt acacactgct tcaagtgtgt
     7201 aaacatatag ccatatgccc atttaggccc accatgaatg aacaatacag caggatattt
     7261 ttcgccttct ttagcatcta taggtggtat aacaaaacca tcaacttctt ctttattttt
     7321 tgctacaaac gtcaattttt caggtaatga aaccttatga gtatctaaat atggtttaga
     7381 aaaagcagtc atttttttca ttgtacctgt agtcatatca atgctatata tttctgctaa
     7441 ctcataatct ttcaacatta ctgtgtacat ggtgttgtcg taaatatcaa aagatgtaaa
     7501 agcatctact tttgtgatga tacgtatatt acctgtcttt gtatcaaact cattaaattc
     7561 tgtcatagtt ctgcaaattc ttgcaccaat aaatttatct cctataactt tattactacg
     7621 tccaccacca tatactgcat ctacatccag taatgtagca tacataccat ctggactagc
     7681 aatcatattt atttcactag tactcaaatc aagtataaaa atatcatgat tctgtgtaat
     7741 agaacggtct aatgttgaag cttgcaacat tactttatct ttgccaatat attttgccaa
     7801 acttatttgg tactttcctt gtggtacaag ttctttggtt tcaccactgt ctatatgata
     7861 ctcccaaagt ccagaaaaag gagttgcaca atcttcataa atctgaccag tatataaaat
     7921 tctgttttta tccgaagaaa catctatacc tgctacttca caaaattttg gtgtaatggc
     7981 ttttgttata ttttcacctt cttgatataa ttctaatgtt acacgtcttc tttgagaaaa
     8041 attttctcca tctcttataa aaggtttatc tgtaaatgtc caataatcta caccctctac
     8101 tgccctatct ggttctgatt ttttagaatc cgttggattt tttgttgtca gtaaccacaa
     8161 tttatttcca agtgatacag gtacgctaca agcttttgga atagaaaatg ctttttttgc
     8221 ttctcctcct tctatagaaa tagtgtaaaa atctgttgta cctgcttttg gcttatttct
     8281 tttagatgta aaaagtagtt ctgtattaga aatccaacta aatgatgtta catcttttgt
     8341 ttgagtcata tgtatttgtt tttctgtttt gatgtccatc acatataagt catgttcata
     8401 gcagtctttt tcctcgttga tatttgatat aatataagca atatgctttc catcaggtga
     8461 acaagaaata tcatgaggga atatatattc aaaaatatct gaactttcaa tctttatttt
     8521 ttccattttt atattctcct ttctactgtc ctatgttatc tttttttata tataccgaaa
     8581 accaatctag catttcaact aatctttgta gcttttgttt tggtctacca gaacgagaca
     8641 gttcatggtt ttctccttca aatagacaaa gttttgtagg acacccatga attttcaatg
     8701 ctgaaaacat ctggaatgct tcttgcatat agcatctata gtctttttca ccatgtaaaa
     8761 ataaagtcgg tgtaacacat ttatcaacat aagcaagagg tgactgtttc cattgaagct
     8821 cgtggttttc catatgagaa gcacctgttt gtcctttgca gaaataataa ccaatgtctg
     8881 ttgtaccctc catgccactc cagtttgcaa tagaacgctg agcacaagca acagcaaaac
     8941 ggtttgtatg accaattatc caattcgtca taaatccacc ataacttcca ccttcaacag
     9001 ccaattttgt tttatcaatg tctgtatatt tttcaagtac agtatctgta aattccataa
     9061 aatctatata atcaatttct ccaaactgtt tcctaatatc tgcaaaagca ttgccacgac
     9121 cttcagaccc ccttgggtta cagaagaata caaagtaacc tgcacttgta aatacctgta
     9181 tctcatgcat aaaatatggt ccatatgtca atcttggtcc tccatgaatc gccaatacag
     9241 ctggatattt tttccccacc tcataatcta ctggtttata tacgtatcct tgtatttccc
     9301 atttagaact tccttttgac tctatgtatt ctggcacaga aattttatat tcctctaaaa
     9361 tatggtcatt taaatgtgtt ttcttacaca atgttccatt ctctactgta taaacttctg
     9421 gtaatccatt tcctaaacaa cccacacata caatattgcc atcttttaca tctataaaat
     9481 caactgaacc tgtctgtgtt actttttcct gttttcctgt tggaatatgt attctttcaa
     9541 taatgcagtc catatctact gtagaagtgt aataaatata ttctccatct acctttgttg
     9601 tctgaccagc acccatttta acatcagatg ttactgtatt tccaatacaa tgatgttgat
     9661 gccctgaaag aagtttcatt tctttcgtat caatatcaat agtataaaaa tctccatttt
     9721 ctccatttcc ataagataaa gcatcagtta ggtttagtat cacttgattt tgatataatt
     9781 caaaaccttt tataatatac ttatcttttt ctaatataca gacatttgtg tttgtgtttg
     9841 tatcaaatac atagacccca tttttcaaac cttttacatc tttaaattct tgccctatat
     9901 ataagatttt tccatcactt attttcatac ctacaacatt aaacataggt gctgtaattg
     9961 ccttcaactc atttatatta ctatcgtata catataggcg ttttcttttt ctgctaatat
    10021 atccctcccc attgacagca taaggaattt ctttgaaatg ttcataatgt cttttgttat
    10081 aagcatttat ttttttctgt tgttcttctt ctggcaatcc ctctacaata ggtcggtcat
    10141 tgtcataaga atcaataact gcatacaaac cttttcctat aggattcaat ttaccacctt
    10201 tgatagggag tctaaaaagt tcttctgctt ctccaccatt taaagataaa gcatagtaac
    10261 tcatgcattg cttaccgttt ttgacagctt cttttacttt ttcatttcgc aaagcaggga
    10321 acaagatagt attttcatct tcccaggcat atgctccaac agagtttata gacgttattt
    10381 gttttaattc gccagtttta ttgtttaata tataaacttg gcttgtgtaa ccattctctt
    10441 tgatatcagc tttttttaca ataaatgctg tatatcctcc atctggtgat atttggcaat
    10501 cacttataaa ttgatattcc aaaaatgttt cactcgtaaa tagctttttc atagtatttt
    10561 ctatcctttc tttagtaatt tttttcaaac tccctaccac ctctttcatt ttttatttta
    10621 aatttaaaat aaaaaatttt aaatttaaaa taaaaaattt tataaaggtt atttttttag
    10681 gaagccttct ggcttccttg taatatttgt aatttaaagt atatttaatc catgcttaat
    10741 tcatgcttaa ttcatactta atctatactt aatctatact tagtccatac ttaatctata
    10801 tttaattcat acttagactt atttacacta tattta
//
LOCUS       DAMJZE010000130        10809 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJZE010000130 DAMJZE010000000
VERSION     DAMJZE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.133"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            219..683
                     /locus_tag="PC541_002793"
     CDS             219..683
                     /locus_tag="PC541_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2840241.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            690..1184
                     /locus_tag="PC541_002794"
     CDS             690..1184
                     /locus_tag="PC541_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2840242.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1200..2225
                     /locus_tag="PC541_002795"
     CDS             1200..2225
                     /locus_tag="PC541_002795"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2840243.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2212..4659
                     /locus_tag="PC541_002796"
     CDS             2212..4659
                     /locus_tag="PC541_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2840244.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4839..6212
                     /gene="radA"
                     /locus_tag="PC541_002797"
     CDS             4839..6212
                     /gene="radA"
                     /locus_tag="PC541_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2840245.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6215..7285
                     /gene="disA"
                     /locus_tag="PC541_002798"
     CDS             6215..7285
                     /gene="disA"
                     /locus_tag="PC541_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2840246.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7374..8468
                     /locus_tag="PC541_002799"
     CDS             7374..8468
                     /locus_tag="PC541_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2840247.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8597..10774
                     /locus_tag="PC541_002800"
     CDS             8597..10774
                     /locus_tag="PC541_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840248.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4516 a         1095 c         2006 g         3192 t
ORIGIN      
        1 atttaaagtt ctgaaatcta atcatgaagc aaaaaattag catttggtta tcaataaata
       61 taataaataa aaactcataa ttaatgaaag aataaattca tatctaaatt tctataaaaa
      121 atttaaattt atacaattta gggctttaaa aatttaaata ataggtgtaa aataatagtc
      181 aaagaaagtc aaaaataaaa aggtgtgtgg tgatattaat ggcaactatg acagatataa
      241 ttgaaaaatt tattaaagat ttgatggaag aagataatag catacaaatt caaagaaatg
      301 aactagcaaa cttgtttagt tgtgcaccat cacaaataaa ttatgtctta acaacaagat
      361 ttacaataga tagaggatat tacattgaaa gtaaaaaagg tggcggagga tatgttcaaa
      421 tagaaaaaat acgaaaaagt aaagatgggc atattagaga actactaaat gaaaaaatag
      481 gaagtcagat atcctataaa aaagcaaaag aattattaga aggtttaaaa gagtcggatt
      541 tgattaacga aagagaatta aaattaatat tatatgcaat agatgataaa tcattgtgta
      601 tgccaatata tgaattaaaa gagaaggtaa ggtctaatat tttaaagaat ataataatag
      661 gattatttag tatagaggag tgataattta tgctatgtca aaaatgcaat aagaataaag
      721 catctgtata ttataataag atagtcaatg gagaaaaaac agagatgtat ttatgtagtg
      781 agtgtgcaaa agagaataca gaaatgaatt ttaacttaga tatgcctttt tcaatgatgg
      841 atatattttc taatttaggt tttcaaccta aaaaagaact tgaggaaaag ctagtttgtc
      901 ctaaatgcaa tacaacatat agtgagttta agaataatgg gagatttgga tgtagtgaat
      961 gttataatgc atttagtagt caagtaaatc caatgcttca aaatatacat gggcatatag
     1021 aacatacagg aaaagcacct aaaaaatctt tttataagat aagtgtagaa aatgagatta
     1081 aagaactaaa agaagattta gatagagcta taaaaaatga agagtatgag ttagcagctc
     1141 aatttagaga taaaattaaa tatttaaaag gttctattga ctaagattgg agtgatatta
     1201 tgaaagagaa catagtcatg aaatcaagag ttaggctagc tagaaattta aataactatc
     1261 catttccaaa taaattagat aaagaatgtg ccatggaaat aatagaaaaa gtaaaaaatg
     1321 cgtttattaa ttctaactta gaacaaaaag aagaatttga tttctataaa attgaagatt
     1381 tagaccaatc taaaaaaatg cttatggttg aagaacatat aataagccca gatttagcag
     1441 aaaatgataa aagtgcagtt atagtaaaaa aagataaaac tataagtata atgataaatg
     1501 aagaagacca tataaggata caaactatat gtgatgattt aaacttagaa tatgcatata
     1561 gtgtagcaaa tgaaatagat gatttgttag aatcaagttt agaatatgcc tttaacacaa
     1621 agctaggtta tctaacatct tgtccaacta atacaggaac aggaatgaga gcttctgtaa
     1681 tgatgcattt accagcatta agtcaactag gctatatgga tgaactttat aaaatttcat
     1741 cacaaatagg tatagcaatt agagggatat atggagaacg aacagaagcg ttgggaaata
     1801 tatatcaaat atctaatcaa cttacacttg gaagaacaga gtctaatatt atagaaaatg
     1861 taagtggact tacaaaagat gcaatttcaa aagaaataaa agctagagaa atattgcaaa
     1921 aaaaattagg tataaaacta gaagataaaa tatttagatc gatagggaca cttgaaaatt
     1981 caagagtaat gagtagcgca gaggcaatga gccatctatc aaatataaaa atgggaatag
     2041 aaatgaacta tatagataaa ctagatttaa gggctataga acaacttatg ataggtatac
     2101 aaccagcgca tcagtcaata atgtataaga gtgatgatgt tgagaataga gatataaata
     2161 gagcaacata tattagagaa actttggaaa aattaagagg agggatgaat tgtgaacttc
     2221 aatagattta cacaaagagc taaaaaggca attgatttag cgtttgaatc tgctaaaagt
     2281 ctaggacaca atattgtcgg aagtgaacat atacttttag gtcttttaag agaagaagaa
     2341 ggtatagctg ctaaagttct aagtaaagtt ggatttacag aagcttattt agaaggcaaa
     2401 atagttgata tggaaggtaa gggagaagaa atctcagaag atatagtatt aagtccaagg
     2461 agtaaacaaa tacttgaatt atcaggaatg tttgcaaata aattaaaaac aaattatatt
     2521 gggactgagc atatattatt agcaattatt caagaaggtg aaggaatagc taataagatt
     2581 ttaaattatg ctggagtaaa tgataggact ttagcccaat taacaattga tatgatgggt
     2641 attagtgata aaaatcaata caaagcagag aatagttaca cgggcaacca gaatcaagca
     2701 gaatctaaag tacttgataa gtatggaaga aatcttacac tatatgcaaa acagaataaa
     2761 atagaccctg taataggtag agaaaaagaa atacaaagag taatacaaat attaagtaga
     2821 agaactaaaa acaatccagt gttgatagga gacccaggtg tagggaaaac agccatagct
     2881 gaaggtctgg ctacaaatat agccttagga aatgttccag aaacactaaa aaacaaaact
     2941 ttatactctt tagagatggg ttcattattg gctggagcta agtatagagg agaatttgaa
     3001 gaaagaatta aagaagttgt agatgaagtt gttaaaaatg gaaacataat cttatttata
     3061 gatgagatgc acacaataat aggggctggg tctactggag aaggctctat agatgcttca
     3121 aatatattaa agccagcctt agcaagagga gaaatacaag ttataggtgc aactacaatt
     3181 gatgaatata gaaagcatgt tgaaaaagat tctgctctag aaagaagatt ccaaccagtt
     3241 atggtagatg aaccaactaa agaggactca ataaaaatat tagaaggatt aagagataaa
     3301 tatgaagctc atcataaagt taaaataact gatgatgcta taaaggcagc tgtagaatta
     3361 tcaactagat atatatcaga cagatatctg ccagataaag ctatagactt gattgatgaa
     3421 gctgcatcaa aagtaagatt aaaagaaaat acacctcctt cagaaataaa aaaattagaa
     3481 ctagaaatag aaaatataga taaagaaaaa gaagaagcag taagatgtca agactttgag
     3541 aaagcagcca aaataagaga tgaacaaggt ctacttaaaa aacaattaga agatgttaga
     3601 gaaagatgga acaagtcatc taaacattca gatttagttg atggagaagt tattgctgaa
     3661 gtagtgggct tatggacagg aataccagtt aataaaattc ttgaagaaga ggctgataga
     3721 cttttgagac ttgaggaaat actgcacaat aaagttatag gtcaagaaca agcagtaaaa
     3781 tctatttcaa aggcaatcag aaggtcaaga gcaggtctta aagaccctaa tagaccaatt
     3841 ggttcatttt tatttttagg tccaacagga gtaggtaaaa cagaattatc taaggcatta
     3901 gcagaagtgc aatttggaga tgaaaatcaa ataattagaa tagatatgtc tgaatatatg
     3961 gaaaaacatg ctgtgtcaag aatgataggt tcacctccag gttatgtagg tcatgatgaa
     4021 ggtggtcaat taactgaaaa agtaagaaga aatccatatt cagttatctt atttgatgag
     4081 atagaaaaag ctcatccaga tgtatttaat attctgttac aaattctaga tgatggtaga
     4141 ctaactgatt caaaaggaag aactgtagat tttaagaata ctattgtaat aatgacatca
     4201 aatgttggag catctacaat tggtagacaa aaaactttag gatttagtat agctaaagga
     4261 gacgaagaag aaaaatctca atatgaaaaa atgaaagaaa atataatggg tgaattaaaa
     4321 caaagattca gaccagagtt tttaaacaga atagatgata taattgtttt ccactcacta
     4381 aatgaaaatc atatatcaaa aatagtactt ttaatggcag cgaaactaca agagagatta
     4441 aaagaaatgg atataaaatt agaaatgagt gatgaggctg ttaaattaat ttctaaatct
     4501 ggatttgact tagaatatgg agcaagacct cttaaaagag ctttacaaaa agagttggaa
     4561 gatgagctat ctgaagcaat cttaaaaggt aatgtgaaaa agggaagtaa tgtagtagca
     4621 aaagtcaaag atgaaaaaat agtttttgaa actaaataat ttaatagagt tgcattttaa
     4681 aatattgcaa atataagttt tataatctaa acaaaatttt aagggcaatg aatctataat
     4741 aattcattgt ccttaattat tttatttaat ctttacatat tgtctttcag atatgctaaa
     4801 attatagtat agaataaaaa attaaggatg ataaaagtat ggcaaaaata aaaacaaaat
     4861 atgtatgtca atcttgcgga tatgaaacag ctaaatggct tggtaaatgt ccagaatgta
     4921 caaaatggaa tacatttgta gaagaaatag accaaaaaag caccaaaaaa gaggtcttta
     4981 taatagataa atcttcatct aaaccagtca gtataaactc aatagagagt aaagaagaag
     5041 agcggtttac aacagatata aatgaattag atagagtgct tggaggaggt atagttaaag
     5101 gctctttagt gcttgttggt ggtgaccctg gtataggtaa atcaacactt ttaatccaag
     5161 tatctagtaa tgttgccaat ttaggaaaaa ctgttttata tataactgga gaagaatcag
     5221 aatctcaaat aaaaatgaga gctaaaagat taggtattaa ctcagaaaaa ttatatatat
     5281 ttgctgaaaa taatttaagt ataattgaat cttatttaga aagtgtaaat ccagaattaa
     5341 taattctaga ctcaattcaa actgtcttca gtccagaaat atcctcggca ccaggaactg
     5401 tcagccaaat taaagaaggt acttcaaaat tcatgaaaat ttctaaaaaa atgggaattt
     5461 caacgttcat agttgggcat gttacaaaag aaggttcact agcaggacca aaattattag
     5521 agcatatggt tgatactgtt ctatattttg aaggagaaag atataatact tatagattag
     5581 taagagctgt taaaaatcga tttggttcaa ctaatgaact tggagtattt gaaatgaggg
     5641 atttaggatt agtagaactt gataatccat ctaaaatttt aatctctgaa aaaccaaaag
     5701 atgtagcagg ttcagtaata atatctactg tagaaggtac gaggccaatg ttacttgaat
     5761 tgcaagcatt agtttctcct acaagttttg gtataccaaa aagaacatct acaggagtcg
     5821 attacaatag agtaggtatg ttattagcag tattagaaaa acgtgttggt ttgcaaatac
     5881 aaaatcaaga tgtctatata aacatagttg gaggaataaa gataaatgaa ccctcaattg
     5941 atttagggat agccatttct gtagcatcta gttttagaaa tataccgata gatgaagata
     6001 tagctgttac aggagaagtg ggacttacag gagaagttag agcagtaagc tttatagaaa
     6061 agagaatagc tgagtgtaaa aaacttggat ttaaaaagat agtagttcct agaagtaatt
     6121 atgatgttgt aaaagagacc aaaggcatag aaatatggcc agtagataac ttaagacaag
     6181 ctataaatat agtacttggg aggaatcaat aaatatggag aattttctag ataataaaaa
     6241 tatgctatat gcattaaaaa tgatatctcc tggaactcca cttagattag gtctaaacaa
     6301 tgtactaaga gctaagactg gtggattaat tgtaattgca acaaacgaag atgtaatgaa
     6361 aatagtagat ggaggatttg ctataaatgc agaatattca ccatcatatc tatatgaatt
     6421 agctaaaatg gatggagcta tagttttaag tggtgatgta aagaaaatat tatttgctaa
     6481 tgcacaactt atacctgact attttataga aacatcagag acaggaacaa gacatagaac
     6541 agcagaaaga gtagcaaaac aaactggtgc tatagtcata ggaatttcac aaagaagaaa
     6601 tgttataaca gtttatagag gaaatgagaa gtatgtagtc gaagatatat ctaagatatt
     6661 tactaaggca aatcaggcta tacaaactct ggaaaaatat aagacagtat tggaccaagc
     6721 tgtaacaaat ttaaatgcct tagagtttaa tgatttggta actatttatg atgttgcatt
     6781 agtcatgcaa aagatggaaa tggtaatgag agttacaagt ataattgaaa aatatgtgat
     6841 agaattgggt gatgaaggaa ctttagtaag tatgcaatta gaagaattaa tgggtacaac
     6901 cagaatagac cagaaattaa tattcaaaga ttataataaa gaaaacacag aaataaaaga
     6961 acttatgaaa aaggtcaaaa atttaaattc agaagaacta atagaattgg tcaatatggc
     7021 aaaactatta gggtatagtg gtttttcaga aagtatggat atgcctataa aaacaagagg
     7081 ttataggatt cttagcaaaa tacatagact accaacagca ataatagaaa acttagtaaa
     7141 ttattttgaa aactttcaac aaattttaga tgcatctatt gaagaattag atgaggttga
     7201 aggaataggt gaaataagag caacatatat aaaaaatgga ctcataaaaa tgaaacaatt
     7261 agtcttatta gatagacaca tatgatgaat tttaaatgaa ttttactact tttttcttga
     7321 atataatata cactttggtc tataatagat aagtataact agggggtgaa gcattgataa
     7381 ggaaagtaac aagaatattg tttattttac ttggatttac aataggtata acaacctatt
     7441 taacattaat gaaagatttt gaaatattaa catttggaaa agaaacatat ggttatatcg
     7501 cagctgtagt agcaggtata ataatagcca ttttaggata tcttatagag ccatgggttg
     7561 taaataaagt aaaagaaatt gctaaaatag tggataaaga attgtcaaaa tatcctcaga
     7621 ctgatatatt gttagggtct atgggtctta tagtaggatt tgttattgcg tatttattaa
     7681 gtggattagt aaatagaata ccaatagtag gtggaatact atccttacta ttatatttat
     7741 tcctaggata tcttggaatg aaagttgcgc ttaaaagtaa aaatgattta tttaatattg
     7801 gaaaactagg aagattagca aatcctatta aggataaaga taaggataaa gaaaataaaa
     7861 aagaagtaaa agccattcct ccaaaagtat tagatacaag tgtaataatt gatggaagaa
     7921 tagctgatat ttgtaagact gggtttatag aaggaaaact tattattcct gcttttgttt
     7981 tggaggaact aagacatatt gcagattctt cagatgattt aaaaagagta agaggtagaa
     8041 gaggtctgga tatattaaat ataattcaga aagaattaaa tatagaagtt gaaataagtg
     8101 agagagactt tgatgatata gcagaagttg atagtaaatt attaaagctt gctcaggttt
     8161 taaatggaaa agttgttaca aatgattata acttaaataa agttgctcaa ttccaaggtg
     8221 ttgaagtatt aaatataaat gagttagcta atgctataaa accagttgct ataccaggtg
     8281 aagatatggt tgttcaagtg gttaaagaag gaaaagaggc tcaacaaggt gttgcatatc
     8341 tagatgatgg aactatgata gttgttgatg gaggaagaaa atatatgaat gagactataa
     8401 aagtactcgt tacttctgtt cttcaaactc ctgcaggtag aatgatattt gcaaaaccta
     8461 aaaattaata tgttcaaaga aacctataat ttgggctgtt tattttaaat agatggtcca
     8521 aatttattta tttataacga taatgtttaa tgtattctta aagatgggga tttaaatcag
     8581 aaagcaggga gaatttatga gaaaaaagat tatatctttc ttagcaacat ttatcataat
     8641 attaacatca gcaagtcagt atagttttgc agatgatata tcaacacgtg gaaaagtaat
     8701 atttatagat atgaatagaa ctagtatgag taatatgtta agaataaaat cattaagaga
     8761 agaactagac aatagagggt atattggctt gatgaatata agaggcgata aaggaagtga
     8821 tgatagaagg tcatatgcta gtatgggtgc tggtggtaga gctaatgtgg ctaatgaaga
     8881 agatattaat tttgaaagct cgagtaagga tagaaatata gtttttgaat cagctacagg
     8941 aaaaagtgca aaaggtataa ataatttaac tataaataag tctataaatg aaaatttaaa
     9001 ctttggagag tatggttctg tattagggtc attaggacaa agtttatctg ataatggatt
     9061 aaaagcatct gtattgggta attcagatat aattgaaaat ggtcaactta taaaaaatag
     9121 aaacttatgt ctaactgcaa tggatgaata tggaagaata cctaatggaa atgtagatac
     9181 tataaataaa aaagacttat ctatgcctta tggaatatca actgattatg ataaattgat
     9241 tgtagagact aaagaagctt acaaaaataa tgatgtgatt tttgtggagc ttggagacac
     9301 atatagatta gatttatata aaccaaattt aaatgaaaaa acatatgaga gtatgaaaga
     9361 taacatagaa aaaaatatag atatatattt aaaaaatata ttttctatgg ttgaagaaaa
     9421 tgacactgtc tacatagcaa gtgcatttcc aagtgattta gattataaaa ataaaagaag
     9481 actttcgcca ataataaaat taaatggaga agggaaaggt atattatcat cttcaactac
     9541 tagaagagaa ggtattgtaa caaatttaga tgttggggtt gaaatattag acaactttaa
     9601 tataaaaaat caaaatatgg ttggaagaaa atatgaactt attaacagag atgataataa
     9661 agaatttttg atggatgaat atcaaaagat agtatctatt tcaagcataa ggtctacaat
     9721 actgaatggt tttgtaagta tagttttctt gtcatggata gttgctatga ttgcaatttt
     9781 atttagaaag catatatcaa aaaattataa agaaacaaca ttttttatat taaaggaact
     9841 tttaaaaata ggaatagtaa tgccactgtc atttatgata acaccaataa tgaatttcaa
     9901 aacaccatta gcaatatctt taggaataat tataataaca cttactatat atcttataag
     9961 taaagtttta ataaagaacg atttaaaaaa catgctgttt tttactggat taaccatagt
    10021 aattatggtt attgatgctg ggtttggctc atatttaatg aaaagcaatg ttatgagtta
    10081 tgattgtata attggagcta gatattatgg agtaggaaat gagtatcaag gtgtggcaat
    10141 cggtagtgca atatttacat ttgcaatttt acttacatat aaaaatatac caaaatggtc
    10201 tataatagtt ttttctttgg ttatcttaat tacaagtgca tctccaatta tgggagcaaa
    10261 tgttggaagt gctatttctg aatgtgttgc atttttacta tttatactat tgatatataa
    10321 tgtaaagata gatttcaaaa aaatcgtatt attaggtata gcagtagtat ttgtattagg
    10381 agtttttgta gcaatagata tgattttggg aagtaattca catttaggca tgtttgtaaa
    10441 agaaatttac tttaatggac caggagaaat aatacaaaca tttagtagaa agatagagat
    10501 gaatttaaag ctagctcaaa ccagtgcatg ggtaaatata ctgttaactg gaataggtgt
    10561 aattttagtt cttatgatta atcagattag atactttaag caactgatgg atgagtatcc
    10621 aattgtattt aaaggattta tagcatcaat agcaggatgt ttagtaactc ttttagtgaa
    10681 tgactctggg attgtgtcat cagctacagc ctttatttat gtaattgtac ctatgataac
    10741 attaagtata aatcttacag cattaaaaga ataagtactg tatgaataat atgtaaatat
    10801 taatacatt
//
LOCUS       DAMJZE010000131        10801 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJZE010000131 DAMJZE010000000
VERSION     DAMJZE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.134"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(5..1063)
                     /locus_tag="PC541_002801"
     CDS             complement(5..1063)
                     /locus_tag="PC541_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2840249.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1130..1501
                     /locus_tag="PC541_002802"
     CDS             1130..1501
                     /locus_tag="PC541_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2840250.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1538..2212
                     /locus_tag="PC541_002803"
     CDS             1538..2212
                     /locus_tag="PC541_002803"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2840251.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2461..3300
                     /locus_tag="PC541_002804"
     CDS             2461..3300
                     /locus_tag="PC541_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2840252.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3544..3640
                     /locus_tag="PC541_002805"
     tRNA            3544..3640
                     /locus_tag="PC541_002805"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3578..3580,aa:Sec,seq:tca)
     gene            3790..4497
                     /locus_tag="PC541_002806"
     CDS             3790..4497
                     /locus_tag="PC541_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2840253.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4639..5475)
                     /locus_tag="PC541_002807"
     CDS             complement(4639..5475)
                     /locus_tag="PC541_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2840254.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5663..6568
                     /locus_tag="PC541_002808"
     CDS             5663..6568
                     /locus_tag="PC541_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2840255.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6577..7761
                     /locus_tag="PC541_002809"
     CDS             6577..7761
                     /locus_tag="PC541_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2840256.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7876..9177
                     /locus_tag="PC541_002810"
     CDS             7876..9177
                     /locus_tag="PC541_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2840257.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9131..10174
                     /gene="holA"
                     /locus_tag="PC541_002811"
     CDS             9131..10174
                     /gene="holA"
                     /locus_tag="PC541_002811"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2840258.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10361..10627)
                     /gene="rpsT"
                     /locus_tag="PC541_002812"
     CDS             complement(10361..10627)
                     /gene="rpsT"
                     /locus_tag="PC541_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2840259.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         4230 a         1114 c         1676 g         3781 t
ORIGIN      
        1 aaacttattg tttatttaat ttttcaatag aaaaagattt atctgtaagt ttaaaattat
       61 cttttatacc cttagttaag tatactttct tatccttagt tatgaatata gcatctacat
      121 tatcaatttt attaattaaa ttcatacctt tttcaagacc aagaccaaat gtagaagtgg
      181 ataaggcatc acaattaata gatttatcag agattattgt aacactactt aaattgtttt
      241 caaaaggata ccctgtaaat ggatttaaca tatgatgata tatttttccg tttttttcga
      301 taaatctttc ataaattcca gaagtaacaa cagacttatt tgtagtttct atgtttccta
      361 tggattcacc atttttacta gttgggtctt gaatacctat tttaaaaggt ttatcatttt
      421 taccattttc taaagtaaat atatttcctc ctaagtttac taaacctttt tttacgtcct
      481 ctgattttaa ataggctaca attttatcag cagcatatcc tttagctatg gcacctaaat
      541 caattttcat attttctttg gttaatttta tagatttatt tttttcatcc aatatgatgt
      601 tcttataatc aataagaggc agaagttttt gtatctcaga atcacttgga actttagctt
      661 tatctgttcc aatagcccat aaatttgata atggaccaat tgttatgtca aaatatccat
      721 ctgataattt ggaatattgt atagcttctt ttacgacgaa aaatgtatca tcagaaactc
      781 tgacaaattt tttaccagca ttatcattta ttttagaaac atcacttcct ggtatatgag
      841 tgctcatttt gttgtcaata tatcttaaaa ttgaatcaca ttcatttaag attttatcac
      901 ttttggattc ttttacatta aatacagtaa cttcgtttac tgtaccaagg tagtagctag
      961 ttttactata tttagtattc tggcttgaac tcctaaaaaa tgaaaataaa attaatgcta
     1021 aaatcaatat cacaaaaata aatgtgattt ttttcttatt catggtatat tcctcctatg
     1081 aaattgtgtt attattttat tataacataa tttcatagga ggacaaaaaa tgaagaaaaa
     1141 agacattctt cttattttag gcattttggt tgttattaca gcatgttatg ggataataaa
     1201 tgttataaat tctaaaaatg ctggaaatat tgagatttat gtagataata aattgtataa
     1261 aactgtttca ataaatgcca aagaagaatt taaaatagag aatagagggg ggtataacat
     1321 agtaaagatt catgataaag gggttgagat agtagatgct tcatgtcctg ataaggtttg
     1381 cgtacatact ggatttataa acaagccaag tcaaagcata gtttgtatac ctaatagagt
     1441 gagcataaaa ataaatacaa atgaaaagaa tgataatcaa gaagatataa tttctaaata
     1501 aaataataaa aataattgaa aggaattggt gataattatg gttaacttag gaaacgattg
     1561 ggatgaatta ttgaaagaag aatttgaaaa agattactac ttaaacttaa gaaaatttct
     1621 tatagaagaa tataaaacac gccaaatttt cccaaatatg cacaatatat atgaagcttt
     1681 aaaacacact tcatacaaag acacaaaagt cttaatatta ggacaagacc cttatcatgg
     1741 agataatcaa gctcatggtt tagctttttc agttcaacca caagtgaaaa ctccaccatc
     1801 tttattaaat atgtataaag aattaaaaga tgatttaggt tgttttatac ctaataatgg
     1861 atatttaatg ccttgggcag accaaggagt gttactttta aatactgctc ttactgttag
     1921 agcgcatgaa gctaattctc ataagaacaa aggatgggaa attttcactg atagagtaat
     1981 atctatttta agtgagcgtg aagacccagt tatttttgta ctatggggaa gtaatgcacg
     2041 taaaaaagta gagttgattg atacaagtaa acattatatt ttggaagcac ctcatccaag
     2101 cccattgtca gcaagtaaag gcttttttgg atgtaaacac ttctctaaga taaatgaaat
     2161 tttgaaaaag ctaggaaaag aacctattaa ttggcagata gaaaatatat aaaagtaaaa
     2221 ggtaacaaaa tgataacaaa acaacaataa acaatgacat tgttgtaact aatagaaata
     2281 tgcttaaata gttgaaaata ctatgtatgc atatttctat ttttttgtca agttgacaac
     2341 tcttgaaaaa atagttacaa tttagttaca caaaaaacac aaataaaaaa agaaacttac
     2401 aaagcagaat tttatgaagc aaaatatata gtgtaaaaaa tcttagggag gaaattttat
     2461 atgaatttta aaaaattgag taatgctaaa gtctcggtaa tggcagttgt tttatcagtt
     2521 ggaatattat cgggatactc tgcgcttaat aaacaagtaa cattagtagt aaggggagaa
     2581 gaaagaacta tatcaacatt tagttctaat gtaggagacc ttttagaatc tcaaaacatt
     2641 agctatgatg caaatgatat agtaacttta gatgaaaata caaagttatc taatggagat
     2701 aaaatagaag taattgatgt aaaggaaaaa acagttgtag aaactaaaga cattccattt
     2761 gaagttacag ttgttgaaga tggctcttta ttaaagggtg attctaaggt taaagaagaa
     2821 ggaaagtctg gtaaaagtga attaatatat aaagaaactt atcacaatgg aaagaaagta
     2881 gaaaaggaat ttgtgaaaaa ggttgttaaa gctgagccag taaataaggt tgtaagcaaa
     2941 gggacaaagg tagaaatgca agtagcctca tcaaggggag aaagttcaag aagaatggct
     3001 tcatcatcat ctagtagcag tagtagtaac aatggtaaaa atatgagagt ggtagcaact
     3061 gcatatactg gtcacagtat aactgcaaca ggaacaaaac ctaagtgggg aacaatagct
     3121 gtagacccta aggttatacc atatggaact aaagtttata tacctcaatt tgacatggtg
     3181 tttactgcag aagactgtgg tggagcaata aaaggaaata aaattgatat ctacatgaat
     3241 gattcaaaaa cagtttataa ctggggaaga aaaactatag atatacgtat attaaaatag
     3301 ttaaataatt tatattttta ctggaaatta tattttgatt atatagagaa gaaactcttt
     3361 atagattcta aaaatataat ttccagtttt ttattttgga tatgtaataa aacaggtaaa
     3421 ttttaaccat gtatagataa aagctattta aattataatt tactatcata taaatctata
     3481 aaaattgttg caaagagtca aaatatatga tattatagat ttcgttgaga aggatttcat
     3541 tatgggagtg gatgggtgcc ggtgtgccct ctagtcttca aaactagtat gaggggttaa
     3601 gagcctctta ggtgggttcg attcccacgc attcccgcca atatgtaaaa ggtatctttt
     3661 aattaaaaga taccttttta aatttgtttt aagataattt agttttaatg atatataata
     3721 ttttgtatta tataatgtaa attttataat actattattg ttcagataaa agtctaggag
     3781 agtgaaaaga tggaaaataa ttttacaacg agaactagtt ttctagttgg agatgatggg
     3841 atagaaaaac ttaataattc aaatataata gtatttggag ttggaggagt tggtagtttt
     3901 actgttgaag ctttagcaag agctggagta ggcaatatta ctattgtaga ttttgatgat
     3961 gtagatataa ccaatataaa tagacaaata ccagccctac attcaactgt aggaagatac
     4021 aaagttgatg tcatggaaga aagaatatta gatataaatc ctaatatcaa tataaaaaaa
     4081 ataagaagtc tttataataa agatactagt gacgaaatac taactgaaag atatgattat
     4141 gttgtggatg cgatagacat ggtaagttcc aaaatacatc taattgaaac ttgtgaaaaa
     4201 aaaggattga aaataataag ttctatgggt atgggaaata aattagaccc aactaaaata
     4261 gtcgttacag atatacacaa aacaagtact tgtcctctag ctaaagttat gaggaaagaa
     4321 cttagggata gaggtatcaa aaagttaaaa gtagtatatt ctactgaaca accaatagaa
     4381 cttaagaaga aagtaatgaa tggtagaaaa gtaacacctg gtagtgtatc atttgtccca
     4441 tctgtaggag gtttaataat cgcatctgta atagtaaatg aactattagg acaataagtt
     4501 ttaaatattt taatttataa attttatata caaatttgaa ggctagaagg atgaatataa
     4561 agcaatttta tattcatact tttagcctag ttattttata ttaccttatt ttatgtagtt
     4621 gtagctttat tttttagatt atgtatctat ttcataatta tattttgtaa atataatttc
     4681 tccatcacta taaaaaacaa tatctcctat tctgtctgtt ctataaattg atacatggtt
     4741 ttgttttaga ttattaataa cctccctatg tggatgacca tattgatttt tatatccaca
     4801 tgatattgca gctatatctg gactaatttt ttttataaat tctaaagatg aagaagaatt
     4861 acttccatga tgacctattt ttataaaatt aatattattt aatttaaaag aatgtaaaat
     4921 ttctttttca ttttcttctt cagcatcacc cataaataaa aaagaattat cattaaaagt
     4981 taatttgaaa actatagaat ttaaattaga ttcttcttgt atatatgatg gacttaatac
     5041 atatatattt atattgttat ctatgtttaa aacatcattt ttatagagat gctgaataga
     5101 taagttttta tccgtacaag aatttattag attttgataa gcctcactat cagttgattg
     5161 ttcaggcata tatatggagt ttacattaaa tttttttata atattatcaa gacttcctat
     5221 gtggtctgaa tctggatgag ttgctattat aatatcaata gtttttattc ttttttgtct
     5281 aagataacta ataattatat tttcgctatc ttcatcacct ccatcaatta atatattctt
     5341 atttgtgggt gtttgaacta aaatactatc accttgtcca acatcaatca tatgtataga
     5401 tagtagagat tttttgtcac agccgattaa aagtgatatt ataataagta ttgataaaaa
     5461 aattttattt ctcaataaaa aatcactcca ttcattacat gtttatataa atttatgatg
     5521 ggtaatataa gatatatatg acttattata ttttatattt taaacaatat aaatgaaatt
     5581 aataccataa taaaattgga gttttttata tatacaaaat aaaattttta aaattaagca
     5641 ataaaaaagg taggaggatt ttatgttttt caaagatatt gaactaaatt caaaaaaaga
     5701 gttagaccca tattttgatt tagtagatta tgaagcttgt gagtactgtt ttagtacttt
     5761 atatatgtgg caacatgttt ataagacagg atattatata ggtgaagatt ttgccgtttt
     5821 agttggggaa tacgaaggag atagcttctc aatattacca cttgctaaaa aagataaact
     5881 tccagaagtt gtagattttg tccttgaata tttttctaaa aataataaaa aaatatactt
     5941 aagaggtatt actactgaag tagtagagtt tttaaaagaa aaatatccag gtagatttga
     6001 gtatatagaa gaaagagatt tatttgacta tatttatgat gcagaaagtt taagaactct
     6061 agctggtaaa aagaatcaaa agaagagaaa ccatataaac tatttcttaa aagagtatgc
     6121 aggtagatat gaagctaaat tattagataa agaaaacttt gatgagtgtt tagtactaat
     6181 gaaggaatgg gaaagtaata aggaagaaaa taatgaattt gatgaaagta tggatgatga
     6241 attaataggt atcaaaaaga tatttaacca ttatgacata ttaaaagata aggtaaaagt
     6301 gtttggtgtt tatgtagatg gaaaattaga agcttttagt ataggagagc ttttaaatcc
     6361 aaatatggca ttgattcata tagaaaaagc taatcctgat ataagaggtc tgtatccatt
     6421 tataaatcaa caatttttgg taagtgaatt taaggacgtt gagtttgtaa atagagaaga
     6481 agatttaggt attgaaggat taagaaaagc taaactttct tatcatcctt gcagatttgt
     6541 tgaaaagtac agtgttaggg aggcttaata gactatatgg agattaggta tgctaaagaa
     6601 gaagagatag aaagtataaa agagatttgg agttattgtt ttaacgatac ggagagtttt
     6661 atgaagtact attttaatga taaatataaa tctgaaaata ctgtagtagc tttagatgaa
     6721 ggaaaaatta tttcttctct tcagctaaat cagtataagt tattactaaa tagcaaggta
     6781 tataatacat catatgtagt aggagtttct acacttccag agggaagagg agctggatat
     6841 atgaacaaag taatgaaatt tactttaaac gaattgtata aaaaagggca attagtttct
     6901 atattgatgc ctattgacta tagactttat agaagatttg gatatgagca ttgttacgac
     6961 cagattgaat atactattaa cacagatgat ttaaaaaatt ttaagtcaag tggaaaaatg
     7021 ataaagtcta atttatcaca aattgatgat ttgatacaaa tagatagaac ttttttaaat
     7081 gaggttaatg gtaatgtgtt aaaagatgaa cattattatg aaaacttatt taaagagatt
     7141 caaagtgaag atggattttt atacattcat gaaggaaatg aaaaagatgg atatatagtt
     7201 tattttcttc aggaagataa aatgtttgtc agagagttat tttataaaaa tatagatgcc
     7261 cttaaatcaa tgcttaaatt tatatataat cacaatactc aatgtaagat agttactata
     7321 tctactccta caatagataa gataagattt atattagaca accctaaaga tagtgatata
     7381 aaaataaaac cttttatgat gggaagagtt ataaatgtaa agaagtttat tgaagatata
     7441 gatattgaga atgatattaa tagctcattt aatttattaa tagaagacaa gtttatagat
     7501 gaaaataatg gtctatttaa aatttctata cagaataaga aggtatctgt agagcagtta
     7561 gataaaaaag gtgcagaaaa gcctcaagaa gattttgata ttaaattaga tataaatacc
     7621 ttaacacagt tatcattttc ttatatagat gtaaatgaag ctattttctt aaatgatata
     7681 aaagatgttt cagaagaaac actagaaaca cttaattgta ttttttctaa aaaaaataac
     7741 tatataaatg aatatatata ggtagagtac gttaaaagat tttaaaaatc ttttgacgta
     7801 tttttttgtt tagataacaa tttatcttgt ttaaaaattt tctatttgaa aataatctaa
     7861 ataatatact tttatatggg agtgattata attcaaataa gaagaccatt attaattata
     7921 ttaatgtttg tggtagctat atcatttatc attacaaata acacaaaaaa cgacgaagaa
     7981 cttaatgata agataataac tatacaagga gttgtcaaag gaaaaattga aaagaaaaga
     8041 tacaatcagt acaaggtagg ggtttttcta ataaatgatt atacaaaaaa taaaaatttg
     8101 aagattggtc agaaagttaa tattacaggt aaattcaaaa gtttagataa aatgaagtat
     8161 gatgattttg attatggaag gtatataaaa agtactggat ataaaggtat agtgtatata
     8221 aataactata aaattattga taaaaataaa ctttactctt taatagggga aataaagttt
     8281 tatattagta aaacttatag gtacttatat aagaaaaact cagattttat aaattctata
     8341 ctattagcag aagttgaaaa tttgactgag gaacaaaaag aaatttttac tagaactgga
     8401 actagtcatg taatatctat atcaggtctt catacaggaa tattatgtgt aattatatcc
     8461 tttctattaa gaggtataaa taaattgtat aaattattaa tactgtcgat ttttataact
     8521 ttatattgta taatggtagg tgcgtctcca tccataatta ggtctatagc atttgttatg
     8581 attttttatt tatcaatatt tattgataga aagaaagatg gaatatcggc attgtctcta
     8641 attgggataa ttttaattat gaataacccc tatgttatat ataatgtaag ttttcaattg
     8701 tcttttttag ctacactttc tatattaata ttctataata aaataaatag tattataaaa
     8761 ttaagtatgg tatcattgac aatatcatca aatatattaa ctttacctat aatttattat
     8821 acatttaaag gtataccatt gctttcaatt ataggaaatt taattatagt accatttgtg
     8881 ggagtaataa tgtatttaag tattgcaagt ttaatcgtat ttaaagtaag tgtagtaata
     8941 gctaaaatta tatctttttt taatagtaca tttatagaaa gcatattttt tttactagaa
     9001 aaaatcagca atttaagttt tgcatacatt aatatagaaa atcctaaatt ttatattgtt
     9061 gttatttatt acataggagt gtttttttac atattttaca tagaaggaaa agagataaag
     9121 gagcaagaga atgaattaca aggatattat aaggaatgta aaagagaaaa actttgaaaa
     9181 aatgtatctt ttttatggaa gagagtatta tttaattgag aatgcaataa aagcatttaa
     9241 agatagtcta aacgagggaa tgcttgattt taacttagat ataatagatg ggaaggaaat
     9301 agtcttaaat cagcttataa gctctataga aacacttcca tttatggatg atagaaagat
     9361 agtaataata aaagactttg aactattaaa aggaaagaaa aagaatttta cagatagtga
     9421 tgagaaatac ttaatagagc atttagacaa tattccagac accacaacta tagtttttgt
     9481 tgtatatgga gatgtagaca aaagaaaatc attagttaag aaaataggaa acaatggaat
     9541 tgtttttgat tgtgataagc tttctgatat ggatttgttt aaatggataa aaaaatcttt
     9601 tgctttaaat gatgttataa tagataattc tcaaatcatg tattttattg aacaagaagg
     9661 atacagagat aagagtagtg aaaagacatt atctgattta gaaaatgaaa taaataaaat
     9721 aagttcattt gttggtaaag ggaataatgt aaccaatgat gtaattgata aattatctca
     9781 aaaaaaagtt gagaatgata tattcaaatt aatcgattat attggagaac aaaatgcttc
     9841 taatgcaatg aaaatattaa atgatatgat acaagaaggc gaatctgtat taggtatatt
     9901 ttctatgata gcaagacaat ttaaaataat tatgcaagta agacagttac aacttgatgg
     9961 atattcaaca aaacttatag ctgataaatt aaaaatgcat caatttgttg taggaaaagc
    10021 attaaagcaa actaaaaatt tttctgatga tataattgtg gaaatattaa actatatatt
    10081 agaaagtgat tataaaataa agacaggtct tataagagat actttagcag ttgaaatgtt
    10141 ggttagtaga tactgtaaaa gagaagcaat ttaaaaaatc tatatattaa taaaaaataa
    10201 atagcaagat atgttatagt tcagagtaga tgtaagctaa attaagtttg atatacaatc
    10261 aactataata tgtctaacta tttatttttt attgtataaa tttacaaaat aaaaaacccc
    10321 tacatagggg ttattatata taagaaatat aaactggtaa ttaagcgttc ataccgttta
    10381 atttttgagc taatttagct acttttctag ctgcagcatt cttatgtata gttcctttag
    10441 aagctacttg gtatattctt ttttgagcgt attggaactt agcaacagca tcctctctgt
    10501 ttccagctgc aacagcatct tcgaatcttc ttattgcagt ttttatttga gattttctaa
    10561 ctctatttaa agcagttttc ttttcgataa cacttattct tttcttagct gattttatat
    10621 ttgccaccgt gttcacctcc ctgtattttt ttatcatatt catcgtcaac atattagatt
    10681 ttaacagaaa taaatctaaa aatcaaggat tattatttat atattttaat aatgtttttt
    10741 attttgattt tttagactgt aaattgtgta taaataattt gcaatatctt gtttttaaat
    10801 t
//
LOCUS       DAMJZE010000132        10735 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJZE010000132 DAMJZE010000000
VERSION     DAMJZE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.135"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            24..791
                     /locus_tag="PC541_002813"
     CDS             24..791
                     /locus_tag="PC541_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2840260.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1009..1222
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1476..2510
                     /locus_tag="PC541_002814"
     CDS             1476..2510
                     /locus_tag="PC541_002814"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2840261.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2551..3768
                     /gene="argJ"
                     /locus_tag="PC541_002815"
     CDS             2551..3768
                     /gene="argJ"
                     /locus_tag="PC541_002815"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2840262.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3783..4643
                     /gene="argB"
                     /locus_tag="PC541_002816"
     CDS             3783..4643
                     /gene="argB"
                     /locus_tag="PC541_002816"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2840263.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4673..5860
                     /locus_tag="PC541_002817"
     CDS             4673..5860
                     /locus_tag="PC541_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2840264.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            5969..6991
                     /gene="argF"
                     /locus_tag="PC541_002818"
     CDS             5969..6991
                     /gene="argF"
                     /locus_tag="PC541_002818"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2840265.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7118..7735
                     /locus_tag="PC541_002819"
     CDS             7118..7735
                     /locus_tag="PC541_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2840266.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7955..8653)
                     /locus_tag="PC541_002820"
     CDS             complement(7955..8653)
                     /locus_tag="PC541_002820"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2840267.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8815..10032)
                     /locus_tag="PC541_002821"
     CDS             complement(8815..10032)
                     /locus_tag="PC541_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2840268.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10223..10636)
                     /locus_tag="PC541_002822"
     CDS             complement(10223..10636)
                     /locus_tag="PC541_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2840269.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         4193 a         1179 c         1802 g         3561 t
ORIGIN      
        1 tgcaattaga aaaggagata agtatgcaga ttattgatat aaatataaat aatattgaca
       61 atgaacatat atgttgttca atttctgaaa aaaaaggaga aaattgtgtt ttttctaaaa
      121 aagcttggat gaaaaataat tttgataaag gtctagtctt taaacgattt gatgtaagag
      181 gtaaagcttt tatagaatat attccagcag aaaatgcatg gtgtcctatt actgcggaca
      241 attatatatt tattaactgt ttttggatat ctggaaaatt taagggtcaa ggatattcta
      301 atctattgct acaagagtgt gttgatgatg caaaatcaaa aggtaaatat ggattagttg
      361 tgctttcgtc tagtaaaaaa atgccttttt tatctgatgc taaatatcta aaatataagg
      421 acttttgttt agcagataca tcagaaccaa attatgagct attatatttt ccttttactg
      481 ataatgctcc tattccatat tttaagaatt gtgtaaaaaa aagaaaatta aatgagaacg
      541 gtatagtttt atattacagt aatcagtgtc cacatacgga aaaatatgct cctattttga
      601 aaaaaattgc aaattctaga ggtattgatg taaatcttat aaaattcgaa acagcaaaag
      661 aagcacaaaa tgctcctgca ccatttacta catacagtct tttttttaat ggtgaatttg
      721 taaccaacga gatactttct gagaaaaaga ttgagaaatt tttagatacc aacattaaaa
      781 aatttaacta aaaagagatg taaatattgt attttgaaaa tatattattt tataatttaa
      841 tatattttgg atgtttttta aattgacgaa tgaaatataa aaattatatc gcaatttaga
      901 tacaaaatgc tggaaattaa ccatatagaa aatatttcca aaaatttaaa aattatattg
      961 acaaagttaa aaaaataaac tatcatataa aacaataaga aaataaattg aaatcctgtg
     1021 aaaaagagag tagcataaag ctagttgata gagagctggg aatggtggaa gcctagtaac
     1081 gaattttatt gtgaatagag ctttggagga gattaccaga agtaatagta tggtaattgg
     1141 tgaatctcgc cttaagagat tagagtgggt aagattatct tatcaattag agtggtaacg
     1201 cgggtgtata ttcgtctctt ggtctttgac caagaggctt ttttaatttt aaatgtacat
     1261 tttgtagata ctttttaatt tgagttttaa tgattattgt aattttacca ataagagtgg
     1321 taccgcggaa aatcgcctct tagtatttat aatgctaagg ggttttttgt tttaattaga
     1381 gtattttaat ctacgtatta tcaaatgatt atcatatagt aaagtggaaa gataggattt
     1441 taagactatt tgaatataaa aatggaggga acaaaatgaa taaaataaaa gttggtatta
     1501 ttggtgcaac tggatatgtc ggggcagagt tgattagatt attaatgaat catgataaag
     1561 tcgaagtgac agcgataggt tcaaattcgt atgttggaaa agatatagtt gatatatatc
     1621 caagtatagg atataaaaat aacatgatat gtatagaaaa tgaaaaagtt atagatatgt
     1681 gtgatgttgt atttacagcc cttccacatg gagttagtga aaagtttgta ataaaggcaa
     1741 ttaaatcgaa aaagaaagtt atagatttag gtgctgactt tagaataaaa gatgaagaag
     1801 tatatagtaa atggtatgga gtatctttta tagataaaat cttacataaa aaagctgtat
     1861 atggattaag tgaaatatac aaagaagata taaaagatgc agatataatt gcaaatcctg
     1921 gatgttatcc aactagtatt tctttaccat tgatgccatt actaagttct aaattgataa
     1981 aaaataataa tataataatt gattctaaat caggtcttac aggtgctggt agagaattat
     2041 cgattagtag tcactttacg gaggttaatg aaaatatcac tgcatataaa atagggaagc
     2101 acagacatac tccagaaata gaacaaaatt taagtgaatc ctgcaaagaa aaagtaagtg
     2161 tagtttttac accaaactta atcccagtaa ataggggaat cttatcaact atatattgta
     2221 caaaagaaga taatatttct ataaatgata tacataggaa attaactgat tattatgaat
     2281 ttaaagaatt tatagaaatt cttccattag ataaggtagc ttctttaaag aatgttagat
     2341 tctcaaataa atgtgtaata tcattacatg aaaatggaga tacattgata atctgttcag
     2401 ctatagataa tatgattaag ggagcagcag gtcaagctat acaaaacatg aatataatat
     2461 ttggtattga agaaaataca ggtttaaaaa atattgcacc atcattttag aaataaaaaa
     2521 tactaaactc atataattag gaggataaat atggagatta taaagggtgg agttacagtt
     2581 tcagaaggat tttttgcttc agggattcac tgtggcttga gaaaaaataa agaaaaaaga
     2641 gatttagctt tggtttattc agatgtttta tgtgatgcag cagcagtagt aactcaaaat
     2701 aaagtaaagg gcaatcctgt ttatgttaca caggaacaca ttaaaaatgg aaaagcacaa
     2761 gctatcatag taaatagtgc taatgctaat acctttaatg gaaaagaagg acttataaat
     2821 gcttataaaa tggctaaatt caccagtgat aaattaaata ttaaagaaag tgatgttttg
     2881 gttgcatcta caggtgttat aggaaagcca ttaaatatag atttaataga agaaaatatg
     2941 gatgaattag tgaataatct aagtaaacaa ggtcatattg gagcaagaga agctattatg
     3001 acaactgata ttataaaaaa agaaatagca gtagcaataa tgtatggaga taaaaaaata
     3061 actattggag gtatggcaaa aggttctggc atgatacatc ctaatatggc aacaacgtta
     3121 ggatttataa ctacagatgc aaatattgat ggagttttgc taaaagaagc tttaaaaatt
     3181 gctgttgaca aaagttttaa tagagttagt gtagatggag atacaagtac taatgatatg
     3241 gtaatgattc tagcaaatgg aaaagcaaaa aatgatagaa taaacaaaaa agatgaacat
     3301 tatcaagtat ttttgagtgc acttacatat gtctgtatag aacttgctaa acttgttgca
     3361 aaagatggag aaggatgtac aaaactaatt gaatgctata taaatggagc attaagtgaa
     3421 gaacatgcag taaaattagc aaaaacagtt atatcttctt cattagtaaa gacagctgta
     3481 tttggagctg acgcaaattg gggtagaata ctgtgtgctt taggttatgc aggtgaagaa
     3541 atagatatgg aaaaagttga tattattttt gagagtatga aaggatatat tgaagtatgt
     3601 aaaaatggaa atggattaga ttttaatgag gaaaaagcaa aaaaaatcct tgaagatgac
     3661 gaaataagta tattagtaga tttaaatatg ggcaatgcca gaggaaatgc atggggatgt
     3721 gatttaagct atgattatgt aagaataaat ggaagttata gaaattagga ggatatactt
     3781 taatgattaa tatagaaaaa gcaaatacac ttatagaagc tttaccatat atagaaaaac
     3841 atcagggaaa gactattgta gttaagtatg gtggaagtgc aatgaaaaaa gatggtctaa
     3901 aagaatctgt tatggaagac cttgtgttaa tgagttatgt tggaatcaat atagttttag
     3961 tacatggtgg aggggcagaa atcaataaga tgctagcaaa agtggatata gaaagtaaat
     4021 ttgtaaatgg tcttagatat acagatgaag agaccatgga aattgtaaaa atggtacttg
     4081 ctggaaaggt taataaagac ttagtaaata aaatacatac aaaaggtgga aaagcagtag
     4141 ggctttgtgg aatagataat aatatgattt tatgtgaccc atataaaaat tatgaacttg
     4201 gatttgtagg agaaatcaaa aaagtaaatg ttgagcttat agaatcttgt ctaaagtcag
     4261 gttatatttc agtaattgca actataggtg ttggagatga tggagagact tataatataa
     4321 atggtgacac agcagcttct gctatagcta aagagttaaa tgcagataaa ttgatacttc
     4381 ttacagatgt ccctggtttg ttaagagagc cagatgaaga aaaatcatta ataacagaag
     4441 ttatattaga ggatgttgat aaattatttg aagaaggtat aattactgga ggtatgatac
     4501 ctaaaataga gggatgcgta gatgccttaa ataacggagt aaatagggta catattttgg
     4561 atggaagagt tccacattct ataataacag agctatttac tgatagtgga ataggtacat
     4621 taataagaaa agaaactgaa taaaattata agtttaagag gggagaataa gaatgaataa
     4681 cgacaataca atttcaaaat ggaatgaata ttttatagat acatataacc aacccaattt
     4741 tgtcatagat tatggtgaag gaagttgttt ttttgatact aatggcaata aatatataga
     4801 ttttacaagt ggatatggag tatcttctct tgggtatagt aatagtaatt taaagaatgc
     4861 tctaaaagag caagtagata aattattaca tacttcaaat ttatatttta atgagccagt
     4921 gttgtattca ggagagaaaa taattaattc aagtggaatg gctaaagtat atttttgtaa
     4981 ttctggaaca gaagcaaatg aaacagcatt caaaatagca agaaagtata gtagtgacaa
     5041 atatggtaat ggtagaggaa ctatcataag tttaaaagac tcttttcatg gtagaacaat
     5101 gatgtcttta atggcaactg gtatggacaa ataccataag tatttttatc ctcttcccga
     5161 aggttttaaa tatatagaaa gaaataatat tgaagattta aaaaataatc ttgattccac
     5221 agtttgtgca ataattcttg aagcgattca aggcgaaggt ggagttaatg ttcttgaaaa
     5281 agattatgtt ttggaaatag taaaaatatg tcaagaaaag gatattgtag tgatttttga
     5341 tgaagtccaa tgcggtatag gaagaactgg taaacttttt ggctatgaat attttgatgt
     5401 aaaaccagat atagttaccg tagcaaaagg gttgggagca ggaataccag taggaggagt
     5461 attggtaaat aaaaaacttt caaaagtact aggaaaagga gaccaaggta caacttttgg
     5521 aggtaattta ttagctatgg tggcagcttc agttgtatta gatgagatat ctaaagatgg
     5581 cttttataat gaagtattag aaaaaggaaa ttatataaga aaatctattg aaagttttaa
     5641 taacaaagtt atattaaaga caaaaggtat tggtctaatg ataggaattg aaactaatat
     5701 tgaatctagt attatagaag agaaagctag aaaaaaagga ttgcttattt tgactgctgg
     5761 aaaaaatgta ttgagatttt taccaccatt aacaatcagt tataaggaaa tagatgaggc
     5821 tcttgaaatc ctaaaagata tattactaga aattaactaa atattcaaaa aatagttgat
     5881 taaagataaa aattagaagt aaataaaaaa gtaaagtgct aacaagttta attgaattta
     5941 aaaagtttat aagaaggggc gaattaatat gaataatata agtttaaaag gtaagagttt
     6001 tttgacatta aaggatttta caaaagaaga gattagatat ttattaactt tatcaagtta
     6061 tttaaaaact aagaaaaaag ttggtataaa aggcgatttg ctacaaggaa aaaatatagc
     6121 tttattattt gagaaaacat caacaagaac tagatgttca tttgaagtag cagctcatga
     6181 tgaaggagca catgtaactt atctaggtcc aaatgattgt catatgggta aaaaagagtc
     6241 tgtatcagat acagcaaaag ttctaggaag attctatgat ggaatagaat ttagaggctt
     6301 taaacaagaa acagttgaaa gtctagcaaa atattctggt gtacctgttt ggaatggact
     6361 tacagatgaa tatcatccaa cacaaatact ggcagatttt ttaacagtaa ttgaaaatgt
     6421 agataaagat ttgaataaag ttaaatttgt atatgttggt gatgctagaa ataatatggg
     6481 gaactcactg atgataggat gtgctaaaat gggaatacat ttcgtagcac tagcacctaa
     6541 ggaactttgg ccaaatgaag aattggttaa ggaaatgaag gaaatagcca agaaatccaa
     6601 aggagatata atgttgactg aaaatattga tgatgttaaa ggtgcagatg ttatatatac
     6661 agatgtatgg gtttctatgg gagaagagga gcaatatgaa gcaagaataa atcaacttaa
     6721 agattatcaa gtaaatatgg atatgataaa gaaaactgaa aatgaaaatg ttatattttt
     6781 acactgtctt ccagcatttc atgatttaga aactaaggtt gctcaggaga tttatgaaaa
     6841 gttcggatta tcagaattag aggttacaaa cgaggtattt aattcaaaat attcaaaggt
     6901 atttgaagaa gctgaaaata gaatgcatac aataaaagct gttatggttt ctactatgac
     6961 tgatgtaaat atgaacaata atttaatata gatatgtttt aatatagact ttactttaat
     7021 gaaaatttat aaaatatttt aaatatatta ttcaaaaact ctcattatat gttaaaataa
     7081 cttatgtatc aacaaattaa gagggaggat ttgagatatg aaaaaaatca tattagcttt
     7141 aacaactttt ttattagcaa tatctttaac agcatgttca agtgcaaaac cagaagatac
     7201 aattgatagt ttttttaaca gtgcgaaaaa gtttgacttt gaaggcatga ataaggtaat
     7261 ggaaaataat gacgaaaaat ataaggatat attaaaggaa ttagatacaa aagacccaaa
     7321 tgcccaatat gttttagatt atctaaaaca gaatgcatct aaaatcacat atacaataaa
     7381 agacagcgag gttaaaggtg ataaggctac aatcaaagta gaatgtaaat ttatagattc
     7441 aacaccatta cttcaagaaa tagtagctga ggcatttaca aagatgattg gtatgagttt
     7501 ttcaggacaa gacttaactg atgagaaaac aactgaaatg ttagtatcaa ttatgaaaga
     7561 aaaacaaaaa agcgttaaag agacttatgt tacaaaaaat gtagagtttg aatgttctaa
     7621 aaaggataat aaatggataa taagttcagt aaatgatgca gtagctgatg tattgttatc
     7681 taatcttgtt acagcaggac aagaattttc taactctatg aatacacttg aataatattg
     7741 tttaggtgta gtaaagtaat aaatatatga ttatttttat aaataaacat ataaatattt
     7801 atgaaggata ccatttttgg tatccttttt attaatataa tgaaatacat ggttaatttc
     7861 tatattgtca agaaactatg atatatgtta taagtctata ctatttaaaa taaacttata
     7921 acatatattt tttataatat agtgtatgaa aaatttattt aactaatttt agatatttat
     7981 tgacaactga ttcagccaag cattttgatg aatcaacaat atttattgaa ggatatgatt
     8041 tttctaaaac tacatttaaa tcagtacaag caattactat attttctata gattcatttt
     8101 taacatcttc aataagttta ttccaaatat caatattgtg tggattttct ttatccattt
     8161 ttatattttg aataagattg tttaatttta tttgccattc atctttaaat ataaattcat
     8221 gtccattatg tattatacca ttctggtaaa ttgtagactc aaaagttgaa ctagtcgaaa
     8281 aaagagttac tttttgtgag atagtaggta attttttaac tgtttcttct acaatattta
     8341 atattggtat attaatagac tcttttaatt ctttaaagta tatatgtgct gaattgcatg
     8401 gcattgcaat aaaatttaca ccagttgatt caagcttttg taatccatca attatagttt
     8461 ctttcataag gtcatgattt attggacggt caatataaaa tggggtaggt aatgaatata
     8521 tcatcatctt tggaaattca tcatcatatt ttgctccata ttgagattga cactcatcaa
     8581 taattaaatc aacaaaaggg gctgttgacc ttggacccat tccagccaaa ataccaatta
     8641 atttttcatt cataaaaatt atctccttta tatataaata tatttatgta taaaataaaa
     8701 atctataaaa gcataaaatt atattttata atagattata ttgtacactt atataatata
     8761 aatataaagt gattttttag attatatatt ttaatatact ttcttatata agaattattt
     8821 taaatcaaga ttatctagta tttttaaaaa tatttttaca gcagtatcca agtcatctac
     8881 agagacactt tcattaggat tatgacttac gcccccagcg catcttataa ataccatacc
     8941 aatatcagtt atattatcca tttcctgagc atcatggcca gcaccactat aaatataaaa
     9001 aggattaaga tttaaatcta taaaactttt ttctatgatt tttgttattt tattagaaca
     9061 tggtacagga acattttcaa aagcaagttc acttgtataa ttaagttttc tattttcaca
     9121 tatatgtgat atttcattaa atatcttttc tacagaatta tcaagaattt cttgtgaaca
     9181 agagcgtaca tcgagtgtaa attcagctct ttcaggtacc acatttgatg aagatggata
     9241 gaaattcatc tttcctacag tagcaaccaa atcagtagtc gttttagcta ctttttcgat
     9301 agctaataca cattcacagg cacaacatcc agcatcaagt ctcatattca ttggaagtgt
     9361 accagcatgt ccagacttac catttactga tatcttaaaa cttttaaatc ctgtaatact
     9421 tgatacaatt ccagcaggaa gagattcatt ttctaaaaca ggaccctgct caatatgaaa
     9481 ttctaaatat gcatctacat cttttctagg atgcaaattt gttaagtttt ttgtattaaa
     9541 tccaaacttt gttacagctt cttcaagtgt aattccattt tcatcaacag atttaagaag
     9601 ttcctcttta aaagtaccag caaaaacttt acttcccatt aaagaagttt caaatctagt
     9661 tccttcctct tcagcgaaag caattagctt aatattgaaa ggataagacc tattattgtg
     9721 ttttgcttct tcaagaccaa ctagaggcac tattatacct aacataccat caaatatacc
     9781 tccacacttt acactatctt gatgagatgc aactactaat gtgggagcat ttggaaagct
     9841 tgatttatat gtacctataa cgtttccaat gtcatcaatt tcaatatcaa gatttaaacc
     9901 ttccatccat gaactaatta aatcaataga ttttttatgt tcttgagtca agataagtct
     9961 tgtaagacca tttgttgttg aacttatttt tcctaatgtt tgtagataat ccatacattt
    10021 ttttcctaac atatttacct cctaaaaata taatttttat ttaagctaag attagctttt
    10081 atacttataa tttttgaact ttcaaataat tttgtatgta tagtaaaata ctattttagt
    10141 attaataatg atataaaaac ataaagtctg tagacgaaga gcagaagttt taatttaaaa
    10201 atatgaaata tgatttttat tattatagta tttttctata ataatatctc tttccagtta
    10261 gaggataatt ttcaagtgaa aatactttat ggtatccaaa tttctcataa aatttaggtg
    10321 cttgaaaact gaatgtatct aaaaaaacat atttacaacc tcgttcttta gcaatttttt
    10381 cagccttata taaaagttga cttccagtac ctttatatct aagcttctca ctaacccata
    10441 agtattttat agaaagccaa ttaccatgag taacacctat aaaacctgca acaattttac
    10501 ctgctttatc tcgaatatat attcctaaat ccactgggtt tttatcttca agtctagcaa
    10561 gattatattt taataatcct tgaaaaatag tatcttcatc ttgtttttct attttttctg
    10621 taatttctaa ttccatattt gaactttctc cttgtataat cttttgtata ttggtatttt
    10681 ttaataaaat cagtagaaag aatatagttt aacattattc taataggcat ttatg
//
LOCUS       DAMJZE010000133        10734 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJZE010000133 DAMJZE010000000
VERSION     DAMJZE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.136"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            128..1327
                     /locus_tag="PC541_002823"
     CDS             128..1327
                     /locus_tag="PC541_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2840270.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1379..1861
                     /locus_tag="PC541_002824"
     CDS             1379..1861
                     /locus_tag="PC541_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2840271.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYEYEVVHS
                     "
     gene            1968..2285
                     /locus_tag="PC541_002825"
     CDS             1968..2285
                     /locus_tag="PC541_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840272.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2443..3402
                     /locus_tag="PC541_002826"
     CDS             2443..3402
                     /locus_tag="PC541_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2840273.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3517..4302
                     /locus_tag="PC541_002827"
     CDS             3517..4302
                     /locus_tag="PC541_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840274.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            4594..5913
                     /locus_tag="PC541_002828"
     CDS             4594..5913
                     /locus_tag="PC541_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2840275.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(5988..6416)
                     /locus_tag="PC541_002829"
     CDS             complement(5988..6416)
                     /locus_tag="PC541_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840276.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6507..7883)
                     /gene="hydG"
                     /locus_tag="PC541_002830"
     CDS             complement(6507..7883)
                     /gene="hydG"
                     /locus_tag="PC541_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2840277.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(7884..8969)
                     /gene="hydE"
                     /locus_tag="PC541_002831"
     CDS             complement(7884..8969)
                     /gene="hydE"
                     /locus_tag="PC541_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2840278.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9005..10213)
                     /gene="hydF"
                     /locus_tag="PC541_002832"
     CDS             complement(9005..10213)
                     /gene="hydF"
                     /locus_tag="PC541_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2840279.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(10228..10479)
                     /locus_tag="PC541_002833"
     CDS             complement(10228..10479)
                     /locus_tag="PC541_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2840280.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         3973 a         1298 c         1666 g         3797 t
ORIGIN      
        1 tatagtagaa gatgttagta aataatagga tgagttctat tttaatacaa ttaaattaag
       61 cttagtttga aatttgtatc taaaaagtaa agtaatctag aattaagaaa atggagatag
      121 aattataatg gaaaaattat attatacaaa tcagtatata agagatttta cagcagaaat
      181 aattgaaata aaagaagtag aaaataagta tcatgtttta ttagataaga cagctttttt
      241 tcctggtgga ggaggtcagt ttggagacct gggaaaaata gagaatgatg atgtaataaa
      301 tgtgtatgaa gatggaaaaa aaatatacca tgtattagat aaaaaaccta taaagataca
      361 taaggtaaag tgtagtattg attgggatag aagagaagat ggaatgcatc aacattttgg
      421 acaacatgta ttgtctggat gtttttttaa aacttttaat gcaaatacga caggttttca
      481 tttgggaaat gagtatagta cagtagatat agaaggtaat ttagaagagg ataaaattag
      541 agagatagag ttgtttgcca atgaaattat tcgtcaaaat atacaagtag agacattact
      601 tccaacgaaa aaagaactaa aaaagatttg gttaagacgt gatttgccaa atactgatga
      661 agagataaga gtaataaaaa taggtgattt agatacgaat gcatgttgtg gagtgcatcc
      721 aaaatcaact ggtgaattga gaatgataaa aataaagaaa tgggaaaaac ataagtcaag
      781 tacaagaata gagtttctag caggtaaaag ggcagtggat gaagtattga agagggatat
      841 atatttgact aaaatatgta gatatttaag ctgtagtgag gaagatgcta taaatggtat
      901 tgtaaatcta aatagtaaag tggaagaggc tttaattaaa aaaagaaggc ttgaagaagt
      961 agtatctaca tatcaggtca aagaaatgat tgaaaaagca aataaggttg gcaatatttc
     1021 ggtcattaaa aaaatttatg atgatgaaga tttgaaatat gtaaataaga tagcaaataa
     1081 aattgcagaa tatgaaaata gtattgcatt attagctgtt aaatttaatg ataaaataaa
     1141 tttagttttt gcatgttcta aaaatttaaa agatattaat atgggagttt tattaaagga
     1201 tgcaataaat cttatagatg gcaagggtgg tggaagtaaa gtattagctc aaggaggagg
     1261 taagaataat ggaaatttag aggctacact tgattatgca tttatgaaga tagaaaaaac
     1321 tctataaata tattatataa aaaaccttag tagataaaat agatatggag gaataagtat
     1381 ggagcaacaa aaggactaca tagagttata tgaaaatgaa gaactaatag agataagaga
     1441 agcacgtatg gatgatttgg atacaatagc aaaatttaat tataatttgg ctaaagaaac
     1501 tgaaggaaaa gaacttgata tggatgtttt aactaaaggt gtaaaagcat tgttattaga
     1561 tgagagaaaa ggaaaatatc atgtatatac agtatttgat aaagttgttg cacagattat
     1621 gtatacatat gagtggagtg attggagaaa tggaaatttc ctttggattc agagtgtata
     1681 tgtagataag gaatatagaa gaaaaggtat ctttaattat ttatttaatt atataaaaaa
     1741 tatatgtgat aaagatgaaa atatagttgg aatgagatta tatgtagaaa aagaaaacat
     1801 aaatgcaaaa gcaacatatg agtctttaaa tatgtatgaa tacgaagtag tacattcgta
     1861 atataaattt gtaatactgt tatactgatg ttataaagta ttgattatat tgacgattat
     1921 atgaaataaa ctggatatta taattagaat ttttaggggg tataaaaatg ggcgttttaa
     1981 aaggaatgtt ttatgtattt ttatttttag atttagtgtc aatattctgt ttctttttca
     2041 ataaaggaaa aatagcatct aataaaattg tgtttaatgc aataggagta cttacatttg
     2101 tactttgttt tatgcttttt agttattatc ctaataataa tcttattgga aaatttattg
     2161 cttctctatt ttttatattt ggagttgcag gagttatgtt gaaagaaaaa aattttttat
     2221 atgctagact tcttttaact gtagttatag ttttttctac attaagattg ttcgttattc
     2281 aataaaatgt atttgtataa aaatttatta aagtctacac atttaagtgt aggctttttt
     2341 gattttttgt ttgaaaatta aggaaatttt aaggaaattt taaaatcgtc ttaaatattt
     2401 attgatatac ttaaaatatt aatagcgaaa ggagacttat cagtgataga aagtaaaaag
     2461 tcagaaaaat tgttactgtc aattatatac ataatatttt tggtatatat tttaatgatt
     2521 ataagaataa ttttatttag agatgtacca atttacgcta ttttcaaggg aacgtttagg
     2581 aatgtaaatt taattccatt ttatacaata tatcaattta tagtagatag taatcttgac
     2641 tttatgaaag ctactgtaaa tattatagaa aatataggta tatttattcc tatgggaatt
     2701 tttttaccta tagtatgtaa aaatctaaat aaaaaaacta taattatagt aattatactt
     2761 gttagcctag catttgaatt aacacaatat atttttgctc ttggaagtag tgatatagat
     2821 gatgttattt taaattcatt aggtggcata attggaatta atatttacat aaacatgaat
     2881 aaattatttc ccaatgatat taacagattt aaggctataa taggtactag tttagtttta
     2941 ggagttatag gattaggagt tatttctaaa aattatcata acttactaac ttttaaattt
     3001 agacctgata gaaaaatatc aaaaatatta attgaagaaa atagggaaat aattaaagat
     3061 ataaataaag atacagtaga tatagttggg acatttgaga gctttaaaaa aggaataata
     3121 actattaaag caggttctaa taacaaagta aagagaccag agaatttaat tgattctgat
     3181 ggaaatataa gaatttattt aaatgaaaat acaaaattag tatcttatat aattaccaaa
     3241 gaatcaaaaa tggatatagt taaatatgag gaatttgatg tgaagaattt agatttatta
     3301 aggagatatg atactattaa tgtttggatt gataaagaaa atcagccaaa ggataaacat
     3361 ggtattatgg ctagtaaatt attaattgga ttgtatgaat aaaatattaa acaagaataa
     3421 aagaataaca aatataaaaa acgaaaaaac tatttctata attattttta cgttgtgcat
     3481 aaaataagaa cattagtgaa ggagtagaga taaattatgg agataataaa atatatcttg
     3541 tactttttta tatattcatt tttaggatgg acagtagaaa gtataggatg ttctatagca
     3601 agcaaaagaa taatcaacag aggtttttta aatggtccaa tatgtcctgt ttatggtttt
     3661 ggagcggtaa ttgtgatatc actattggga agatttaata atgtagtaat agtatttcta
     3721 ttaggaatga ttttaactac gatactagaa tattttacag gatttatttt agaaacctta
     3781 tttcatgcta aatggtggga ttattctgac agaaaattca atataaaggg tagagtatgt
     3841 ttaaaaaatg caatatactt tggtgtaatg tctgttttaa taataagatt tatacatcca
     3901 tttataaaat attttgtaag tataattcca tatagaatat taatttcaat ggctataatt
     3961 acaacacttt ggactatact tgatttgatt gttactatta taacacttaa aaaattagat
     4021 ataaagttaa acttactaga cgatataatt acagacctta atgatataaa tgtgaaattg
     4081 gataaatttg atagaggaga aatacaagct ttatttaaaa caataaataa cgacgaatta
     4141 gaagttagag aaaaaataaa caaaataaat agtaagttgg accgtataga gtctaatatt
     4201 attttacaaa aaagagttat aaaagctttt ccacatataa aacataaaaa acaacaagag
     4261 caactagaac attttaaaaa aattattaat gagaaaaggt aattatacaa tttaatgaag
     4321 acgaatagaa taatggtatt tataagatga ttttcagagg actctataaa aatagaatag
     4381 agtcctttat tttttgaaaa ataatgttta tttgataaaa ataaaatata ttgtttctga
     4441 ataacaaata ttttgaaaat tataaaaaca tatattgaca atttgtgtca aaaatactaa
     4501 cattaagtat ataaagaatt ttatatgaga atatctttat aaatcgcaat aattttaaaa
     4561 cataaattta tttaaagagg ggatgatttg ccaatgagcg agataactag tcaaatgata
     4621 agtacagaag aaaaaaaata tgttgctaaa actcaaaaga taccttatta tccagtagca
     4681 tttaaatcag gtgatggtgc aatgctatat gattatgaag gtaatgaata tgtagatttt
     4741 ttagcaagtg caggctctgc aaatgtaggt catggtaata aggaaatctc acaagcagtt
     4801 aaagaacaaa tggatgatat aacacaatat acacttgcat atttccatag tgatcctcct
     4861 gtaaaattag ctgagaagct tgtggaaata gcccctggag ataatgataa aaaagtacta
     4921 tacagtgcaa ctggttctgc ttgtatagat gctgctataa aattagcaag aggatatact
     4981 ggaagaacaa agataatatc tatgtgtgaa tcttatcatg gaagtactta tggagctata
     5041 tctatatctg ctttaagtac aaatatgaga agaaaaatgg gtccattgtt accagaagtt
     5101 tatcacttcc attatcctga taaaaataga acagctaaag aatgtttaga tgagatagaa
     5161 tatgcttttg cacactatct accagcagaa gaggtagctg caatatttat agagccaatt
     5221 gcaggagatg caggaataat agtgcctcca gtagagtggg tacaaggttt aagtaaaata
     5281 tgtaaagaaa atggaatatt acttgtaagt gatgaaatac aacaaggtat gggaagaaca
     5341 ggtaagtggt ttggtataga aaattttggt gttgaagcag accttatagt tcttggaaaa
     5401 tctgttggag gtggattacc tctaggagca gttgttggaa gaacagaaat aatgcaaagt
     5461 cttgatgcac cagcacattt atttacttta gctggaaaca caacagtatg tgtggctgct
     5521 ttaaaatcta tagagataat tgaaaaagaa aacttacttc aaaagagtat agaaatggga
     5581 gattatatta aagcaggatt tgaaaaacta aaagaaaaat acgatataat tggagaaata
     5641 agaggcatag gattatctat aggagttgat atagttaaag gaaaaggttc taatgaaaaa
     5701 catccagatg ctacagctaa gatatgttat agatgtatac aaactggatt aataatgata
     5761 ttcttaggac aatctacttt aagagtgcaa ccacctctag taataactaa ggagcaagta
     5821 gataaagcaa tgaacatcat agattctgca atagatgatt atttaaatgg tagaattggt
     5881 gatgaagtat atgaagtaac tcaaggttgg taaaataaat attaaatgaa aataggagtt
     5941 atctcaattt tagaaaatta tttctgaaat gagataactc ttttaagtta cccacaaata
     6001 agaaaattgt ttgattcatt aaagactata tttcggatgt cgtttttgtg agtatcagag
     6061 acagtattac gagaataatt acctttattc gtactatctg ttaatttagt tataattaag
     6121 atagctaaaa gaataagaat tactgtgtat aaagcaataa catatggttt tcttttatat
     6181 ttgtatttgt tattgaaaaa agaatggcca taattatttt tatttacttt agatgtattt
     6241 gtattctttt tcttgttacc tttcttttta tcatcctttt ttctattttt agggtaagga
     6301 atgactttgt gtgtattatc aagtttgata tttttatttt tttgaatatt gttttccagg
     6361 ctagcctttt tttctccaaa ttgaatagtt ttattggtat tgttgttatc tttcaatgtt
     6421 aatctccctc ccttaaactt tccctttaga taatattact actaacctat ggaatataca
     6481 ataaataata tataagtaat aaaattttat aggtataaat ctctttctcc acgctctaag
     6541 gcagtcatat tgttagatac tatgtttctt atatcttctc tttctatctt acctatttct
     6601 tctgcgataa gttttttacc cataatttta agttcttcat ctccataatc gattaaaaat
     6661 tcatttaatg tagtaagagc atttggtgta catacattat ggatttgtcg acttttagct
     6721 aagctcatga atctatcgcc agttctacct ttacgataac aagctgtaca ataactaggt
     6781 atatatccat cagtaatcag ttctttaagc acctcaacag ggcttctatg gtctccaact
     6841 tcaaattgtt ctgtattgtc accatcttca tatgctttat atcctccaac acctgttaga
     6901 gaacctgcac taatttgtga tacaccatat tttagtaact catttctcat ttcagcagtt
     6961 tctctagtgg acattatgat tccagtaaaa ggaacagcca atctagttat agctactatt
     7021 tttttgaaca tatcgtcaga aactaagtga ggaaaatctt ctaagctcat accttcagct
     7081 tttttcaatc tagggaaaga tatagtatgg aaacctacac caaatttttc ctctaagtgt
     7141 tcgttgtgca tcataagtcc tagaacttca aatttagggt cagataaacc aaataaaact
     7201 cctgcaccaa catcatcaac accagcttcc atagctctat caaaagctgt taaatggtaa
     7261 taatagtcat tttttataga ttgaccatgc atttttataa atgttggttt gtgataagtt
     7321 tcttggaata atatataagt tcctattcct ttttctttta gtaatttata ctcatctaca
     7381 cttgtagctg ctatgttaac gtttaccctt cttatatttc cgttttcatt atatgttgaa
     7441 taaattgcat ctaagcaatc taagatgtaa tttatatcac aatttttagg gtcttcacca
     7501 gcttctagag caagtctttt atgacccatt ttttctaata ttttaacctc ttcctttatc
     7561 tcttccatgg taagtttttt tcttttaaat ttgttgcatt tactaaaacc gcaataaaca
     7621 cattcattta cacagtagtt tgatacataa agaggggcaa ataaaaccac tctattacca
     7681 tatatttcat tttttatttg cccagctata ctaaataatc tatctagttg ttttttatct
     7741 tctacttcaa gaagtgtagc tatatcttta taactaagtt tttcccttct atcagcctta
     7801 tcaagtacct tttcaatatc atcagaagtt gaattttttg catcttctaa taaagaatta
     7861 ataagttcat ggtttatgaa catttacaaa cgcctccatt caatattatc accgcgagaa
     7921 tagtcaactt caagtcctaa tgacttgatg ttttcttcta aactttggtg atattcacaa
     7981 gcttcttgac cagtaaaggc tttgttatcg taaagagaat attgttttct aaattcttga
     8041 ggagaaaggt ttggcataat tacattgcaa ccagctaaaa gacctgcatt tctaccacta
     8101 gggtttatag atgccaaggc tgttgtagcg ggtaataaaa cttttggaag tagaagtctt
     8161 gtaattgaaa gcataagtaa tgtcttctct aaatctccat gtttataatc cttaaaaatt
     8221 gtatcatggt gtggaataaa aggtcctatt ccaaccatat gaggatttaa ctcttttagg
     8281 tataataaat cacgaacaag gtcttcgttg ttctgaaatg gtgattcaac cataaatcca
     8341 gcacctacct gataaccaat ttctttaagg ttttttaggc attcttttct agttctaagc
     8401 tcaatgtttg gcggatgtaa ttttttataa tgactgtctg tagcagtttc atgtcttaaa
     8461 agatatctat ctgcaccaca taaaaagtat tttttatacg aatcataact cttttctcct
     8521 agtgatagag ttatggcaca atcctcatat ttatttttta tacttgaaac aatttcacat
     8581 attttatcat cagtaaaata tgcatcttca ccaccttgca aaacgaatgt tctgtatcct
     8641 attttataac ctatatcaca acattcaagt atttcatcaa gtgaaagcct gtatcttatg
     8701 atatttttat tagaagattg tattccacaa tagtagcaat tatttttaca atagtttgta
     8761 agctctataa gtcctctcaa gtagactcta tttccataat gtttatttct catgtcgcta
     8821 gcttgtttat aaagatagtt tcttaaattt tgttcgaagt tatattcctc attgtcgata
     8881 ttttctatta aatatagtag ttcatcaaaa cttaatgaat taatgctgta aagtttatct
     8941 ataaagtatt taatcttatt tttagtcata ttcaaatatg taattattaa gtatagttaa
     9001 atttttaata attgaaaagt tcaagtgatc tttccaaaat accttttaca tatgctatta
     9061 agacaccata gtttacaata ggtacattga aagattttgc cttttctatt ctagataaca
     9121 ttccagctct attcatcata caagctccac agtgaactac aagggcatat ttatttatat
     9181 cttcagtgaa ggatactcca gaactaaatt caaaagtaat gtttttgcca gttttctttc
     9241 taatcatatt aggaattttt actgttccaa tatcatcagt ttgtctatga tgggtacatc
     9301 cttcggccat caagatttta tcaccatctt ttaaattttc taaagcataa gctccattaa
     9361 tcaattcttt aaggtctccc ttttgtctag caaacaatat agaaaaagat gtaagaggaa
     9421 tatctttttg tgtatcttta tctacttgtg gaaatacttg agagtcagta ataacaagtt
     9481 ttggtttttt gcctaaatta gatagagttt cttttaaact atcttctttt gtaactatag
     9541 aaattgcacc attatccaaa atatctctta taacttgctg ttgaggaagt ataagtctac
     9601 cttttggagc tgctttatct attggaacaa ctaaaacaac taaatcattt ggttcaatta
     9661 aatctgatac caatttgaac tcagtgctat ccttaggcaa aacttttatt atttcatttt
     9721 ttaaattttc aattccaatt ttatctgttg atgagacaga aacaactgga catttaactt
     9781 tatttttagt taagtttaaa atttctgatt gattcttaat agtatcaatt ttgttaagaa
     9841 ttagaatatg agggatattc ttatcattaa atttttcaat tagtgacaaa tcttcttgtg
     9901 atataccaat ttggcaatcc actactagta aagctatatc tgtcttttct aaaacatcta
     9961 gtgatttacc tattctaagt tcaccaagtt caccaacatc atcaagacca gcagtatcta
    10021 taagaacaca aggtcctata ggaaggattt ccattggtct aaatactggg tctgtagtag
    10081 ttccagcaat atctgagact attgcagcac tttgatttgt tatagcattt attatgctag
    10141 atttacctgc atttctttta ccaaagagac ctatatgtac tcttaccgat tgtggagtag
    10201 aatttagact catttgtact caccacctta aacttctttt tttgatatac tagtttttac
    10261 agatacatga ggtatgttgc ccaatttacc agtcaaacta ttaatagtgt ccatttcgcc
    10321 aacaactgct atacatacga ttgagacacc ttcttcttcg caaggaatgc ccattcttcc
    10381 tttaataata cctttaaaac ctgatacaac ctcattgaat ttaaattgac attcttttgg
    10441 ttcttccaaa atagcactta taactgcaac ctttttcata aatatcctcc taactcagaa
    10501 aatatgtctt aaacaaatcc aaaatcaaaa aacttttctt tgtaaagaaa agtttctaca
    10561 aaagaaaagt tattaattac acaagtataa tgtgctatat taaactattt ccttacagat
    10621 tagataactc ttttctggta gggttagctt gtaaaaattt aatttaacaa caagtaactg
    10681 aagaaaattt ttatacaatt tatataaatt ataataaaaa ttaactaaag aaaa
//
LOCUS       DAMJZE010000134        10716 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJZE010000134 DAMJZE010000000
VERSION     DAMJZE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.137"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(95..427)
                     /locus_tag="PC541_002834"
     CDS             complement(95..427)
                     /locus_tag="PC541_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2840281.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(671..1312)
                     /locus_tag="PC541_002835"
     CDS             complement(671..1312)
                     /locus_tag="PC541_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2840282.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1293..1586)
                     /locus_tag="PC541_002836"
     CDS             complement(1293..1586)
                     /locus_tag="PC541_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2840283.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            1979..2038
                     /locus_tag="PC541_002837"
     CDS             1979..2038
                     /locus_tag="PC541_002837"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2840284.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            complement(2554..3306)
                     /locus_tag="PC541_002838"
     CDS             complement(2554..3306)
                     /locus_tag="PC541_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2840285.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3365..5020)
                     /locus_tag="PC541_002839"
     CDS             complement(3365..5020)
                     /locus_tag="PC541_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840286.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6293..7372)
                     /locus_tag="PC541_002840"
     CDS             complement(6293..7372)
                     /locus_tag="PC541_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2840287.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7456..8112)
                     /locus_tag="PC541_002841"
     CDS             complement(7456..8112)
                     /locus_tag="PC541_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2840288.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8434..8640
                     /locus_tag="PC541_002842"
     CDS             8434..8640
                     /locus_tag="PC541_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840289.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8793..9221)
                     /locus_tag="PC541_002843"
     CDS             complement(8793..9221)
                     /locus_tag="PC541_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2840290.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9697..10635)
                     /locus_tag="PC541_002844"
     CDS             complement(9697..10635)
                     /locus_tag="PC541_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2840291.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3558 a         1909 c         1133 g         4116 t
ORIGIN      
        1 aacttaataa gaagtgaact tagaaaataa agcttaattt caaagactta aatagactat
       61 tattattagt ctaaataaac tacgtctatc aaaattatat attaaattct tctaaaattt
      121 ttattgcaaa tcttgcgttt ggatatgtaa tagacccgcc accaattaca cctattttta
      181 tagcttctat aatttcatct ttacttgctc cagaattaat acaaccctca atatgataac
      241 aaatacactc atcacatcgg cttaaaattg aaatagctag tcccattaat tccttatttt
      301 ttttgcttat agccccttct gaataagtct tatcatcaag acttccaaat actctaaaaa
      361 actcatctgt ttcgcttaat tttttattgt aaaatcccct atattctttc atttctctag
      421 tattcatctt ctacctccta tgtttttctt ataagaatat cacaccgtac ttatattttc
      481 aatattaaat acaaattttc attttatgtt cattagccta atttatatta ttttttgaaa
      541 tctcaataag ttatcatata agtaagattt tttcagaact aaaatagaaa tcttagtaat
      601 ttctttatta tctatctcag ttctgaaata tattattctt atccaaacat tattatagtg
      661 attaattata ttagttattt ttacctgaaa ttttatacat caaataagaa tatataaatg
      721 gtagtattac aattacaaag attacaaaga tattataaaa aggaatatta ataaatgcat
      781 taataattaa taatattcca ccaataagcc aaatccatcc agctaaatgg tgtgtcttat
      841 tccaattatc ttcatcatta agagtccatg gtaaaattat tcctactgag taatttcgtt
      901 tacatttagg aagatagttt ccaaataaaa taactattat tcctaaaatt aatcctgtat
      961 aaaaattaga ctccaaacta actcctgttg aatataaaac catcattatt tgtataatta
     1021 ctgaaagtat agcaactccc cattttgata aattttgtat aacatttgaa aaattagctt
     1081 tcttaggctc attctcaagt ctaaaccatg tatacaaatg gatacatagc attaacaatg
     1141 gtacaccaaa agctgctata acctttggag cataattatc tggatttcca taattatcaa
     1201 aatgaattgc aacttcattt ggtaatctat tataaaaata tattgataca ataaatggta
     1261 taaaacatat taaagtagat aaaataaatt gtttatttat cgcttttttc attatctttt
     1321 tctcccttct ccataaattg tgaaaaccaa agcatgacct cttcgaaaac tgaaatattt
     1381 atttcgtaga aaataaagtt cttatacttt gtttcaaata tgagtcctgc cttttttagt
     1441 tgagatagat gataagaaat tgtagcattc gtcatattaa atttttcact aatttctcct
     1501 gctgacattt tattctccct caacataacc aatatttctc ttcttatagg gtctgaaaga
     1561 gctttgaatg tttctggaaa ccccaacacc atcacctcgc tatttagaat ttcatctaaa
     1621 tagaatataa cataacatcc attttatttc aatagctatt tagaaatttt tctaaatagt
     1681 tatttctaaa tatttattga aaatttttat attaagattc tcaatttact taagtcgctt
     1741 ttatttaatc ttactttatc taattttttt aacttaacaa aagtgtattg caccataatt
     1801 gttaaagcaa aaacctaatg attgaggtac ttatatatat aacaatactt ttaattttaa
     1861 attaaaagta gctaaaccca atttaaaaag tagttaagtc ctatttaaat agaaaagaat
     1921 gaaacgagaa atatactgta taaaagtaca taaaaacatc tactaattag atacaactat
     1981 aacagaatat atttattatt ataataacaa aagaataaaa aaaattaatt tagcataacc
     2041 ttgttaaata tagatttgaa tatgaattta aagttatgta aaattaaaaa tacaggaatg
     2101 aaaattccat tcctgtataa aatctaactt ttgagtctca gtacaatctt attttgagac
     2161 aatctttttt gttttatatt ttcttatatt taaaatataa cgcatatctt atgcaacata
     2221 ttcatatttt tctttatatt ctttcatcgc atcattccaa gcgtcttgta ctgttacacc
     2281 tcttttatta gcaatttttt gtgccagttc aattatcttc atagctcttc ttgatatgtt
     2341 cataaacatt cctcctacaa attaccatat atttcatata tataaatcaa ggttatattt
     2401 ttatgatata taatccttct attgtaagat tacagtttat atgtgtatta tatatgaact
     2461 atattagaag ataattagaa tttttataaa gctatatgat tagattaaat ataaaagctt
     2521 tacaattact tataaattta aaaagtctat aagctaaact aatgtttgat ataccaattt
     2581 tacgaccata aataatgcca ttactaaaaa tattggtttt atcatctttg aacctttctt
     2641 tatagcaact ttagcaccta cataagctcc cattatcatc actaatgcaa acactatagc
     2701 aattttataa tctacttgac catttatcat gaataataac aatgaagtta tattacttgt
     2761 taagtttaat atttttgtat ttgcagaagc atgtaaaaaa tcatatccat atatttttat
     2821 aaatccaaaa gtcaaaaaag ttcctgtacc tggaccaaaa aaaccatcat aaaaacccat
     2881 tataagagcc ataagcatcc ctaatcttaa atttttctta ttaacagctt caaaattatc
     2941 ttcttgacct aaatttttag aaataaaggt gtaaatagcc actattaaaa tcataaccac
     3001 aaccaaaaca tttaatatct cttcacttat tgataataca catctcacac ctagaacact
     3061 accaataata gtaaatggta ctaatttttt taaaaggttg ttattaattt ttccagactt
     3121 tgcatatcta taggcactac ttatacatcc tgctgatgct gcaaatttat ttgtaccaat
     3181 ggcaagatgt actggcactc caattgccat aagaacaggc atgcttataa gtcctcctcc
     3241 tcctgctata gaatctacaa aagcagctaa aaacccccct atacataaaa aaataactga
     3301 aaacataaaa ttcctccatc attctctttc aatttttctt acaatatata ttatataatc
     3361 ttatttaata cttattatcc agcatattct ttctatattc atttggagtt atactattaa
     3421 acttcttaaa tatttgtgaa aaataactac tgttattaaa acctactttc ttacaaatat
     3481 cactaatact catgcttcta tctaacagat attttttaga ttcttctatc ctaagaagat
     3541 ttatatactc agttattgaa actttatttc tttcattaaa aatagaactc aaataatttg
     3601 gacttataaa aaaaacatct gatatactat tcaaatttat attttcttta taatattttt
     3661 ttatatattc gtttatattt tcaattagat ttcctttatc ttttctccaa aatgctctat
     3721 tcttatcaat acactctttg gaaaattcaa ataatacatt ccataaatct tcatcactgt
     3781 ttacaagtga tattttttta ttatatttat ttcttaaata agatataagt tttaaatcct
     3841 ctaaatctct aagcactgtt ctagacaata caaatatcat ttcctgaata gtatctctaa
     3901 catcattata tcttaatttg tttgtatcta ttagattata aacaccttct aaattcatat
     3961 ttttaatttc tttttcaata taaagttcta attcaaaggg atattctttt gaagaattat
     4021 tgaacatatc tatcggatta ataatatact cttcttctaa taatctacta tttgcttttt
     4081 taagtgcctc ttcaagtctt atattttctt ttctactctt caatatctcc tcatctttta
     4141 tactaagttg attttcaaaa aaattcctct tagctatttc ctgaataaat tcagacatca
     4201 ttaataaatt ttcttttaat acatataatc tacttttagg atttaatgga aattcattgt
     4261 aaattttgtt tatgtatttt atatcaatac cagaactact tgataattca gataaatttt
     4321 tatctatatc aatagactta tatactataa aattaccaca ttctatatat ccaactatat
     4381 tttcttctaa tactataggt atctctaaac tggaaacacc atactcacaa tggtacactt
     4441 tttcaaagtt atcattattt atgcttttaa caagtttttt agtacactgt tttatatttt
     4501 tacaaatatc gcaaaattta ttttctttct tagtctcaaa tatttcttta tactttaagt
     4561 catatacaga tattctaagc ttcaatatat tagatatttg atttgcaaga ttttcaatta
     4621 tgtttacatt aactaactcc tgtatattgt tttcagaaaa tactataggt atttgtctca
     4681 aactcgaaaa tttatgagga acataagtaa ttaatattac taaatcaatt ttaccagtgt
     4741 ttataatttc atgttctgaa tatggtgggc aataaactat agatgtttcc tttatattga
     4801 cctttttacc atcaattatt tgtattcctt ctccctgaat cacatataaa agctgttctt
     4861 cacttaaatg aaaatgcttt tcttggtagt ttcccggaaa aaatcttaca attccagcac
     4921 taagtctatt gaattgtgtt tttgatggtt cgtgtatcca cataacctct ccccaatcaa
     4981 atttttgtac ttcattattt gactcactaa aatactccat aagctcacct cttagttttt
     5041 gtctaaatat tttttcttcc cttcttatat taaaataata tcatcatcat taactttcat
     5101 tactataaaa tcatcatact cttttaatac tcctgtaatt ttagttgcac ttagtttact
     5161 acaattcagt tttttaatat attgataaat tctataatta cttttttatt ttgtaaaatc
     5221 agtgttttcc caaaataatt atatctataa atgaaagtat ttttactgct tacatcaata
     5281 tatgttatca taattttcct ttttattgca tatataaata actgtatata acattgtcca
     5341 atactatata gaaatacatc taagaaagaa tattattaaa ttgttccaat tagtattatt
     5401 tttcttatag aaataaaaaa actgttggag tcttttagaa aaattatttt ttacatacct
     5461 tttttgctta gatagtctat cttcacagct attttgtata tacactcttc tagttcatga
     5521 ttttatttta atatcttgct tatattgctg aaataattga attcttttat ctatcatatt
     5581 gtctcttata tcacaaacat attgtttctt atatcacaaa aatgaaagca ttcttatatc
     5641 ttcaatatat aaacctgctt tttttaatct tgtaataaat ataggatact ctatattctc
     5701 ttcctagaaa taactaatac cacttatttt tctattatat agaaagtaat aaatcttttt
     5761 cttaataaaa ttcaatagta agtggaataa aattttatat gaataagaac attataaaaa
     5821 tactttatac aatagtcaat aaaagatatt agcttcaaat tatgttaaag ttgtattatt
     5881 taaagtgaaa aaacaaataa aaaagaaata atagtataag tagaaaaata tacttaaaac
     5941 aaggcgaagc ctctcttgta tctaaatcaa gtagagaatt aaatctaaaa aagaaaataa
     6001 atataaaaga tgactcctgt acattacaag aatcatctta tggtttcata atcactccat
     6061 ggtttttaac tgtctgatat aaaggtctca gttcactcat agcatgacca ccttttccga
     6121 ttgctgaccc cgtcacttat ggacaaaatg tccataagtg atagtcctct cttaaaaccc
     6181 tctaaataga tatatctcaa gcaccgcatt gaaaccagtt gctggaaaca aactgaagcg
     6241 ttctggaata gtaaaagaaa ttgccaccat atccacgtta ctgtcctagt tgttactttg
     6301 ctaacttcgg agcgatttcc tctaacaata cgcagtcaaa ttctgccttt ttgctaagaa
     6361 atggcagatg gtctgcatct ggtataaaaa aaatctgttt gtctggcgcc actatttcat
     6421 caaaaaattt cttcgctgga agataggggc tattatagtc attttctccc atgatgtagt
     6481 atacagggac ctgatagtca actccataat ttcttatgtc agcttcttcc actaaaaacc
     6541 gcaatacatc accttgattg tcaatcgcat ttggattgaa aatggccgta ttttttaact
     6601 ctctcaatga ataatacggg gttgccagta tactgattat attgcccaaa tccatattta
     6661 tagaaagccc atacttgctt tggtatgttc ttactgtcat aagagctgcc aaatagcttg
     6721 gaccatattt tgctggcgga tatggtgcaa tagcttccag cgccttgata tctttttgct
     6781 tccctgcagc tttcaccatt tcaagtgtct tttcatagcc cacgcgctca ttgtccacca
     6841 tattaacggt ctgcgcaatc ccaatgtacg cgctgtaatc ctccggatag gtttgcacag
     6901 ccatagttcc cagaactgtt ccccagctat gacctataag gataattttc tccttgttga
     6961 actcttgctt caaatatgct gtcacctcat ggacgtcttg cagaacacgc tccgcgctca
     7021 tggtctgcaa aactgctgcc ggatcgttcg caaagtatgt tttgcctacg tttcgctgat
     7081 cccagttcac cacagtaaaa tcattttccc actcatactg gtacagatgt aaaaatggca
     7141 tcattgaatt accaggaccc ccatgaaccc aaaggatgat ggggttgtct gcgttttgtc
     7201 cccggatata taacgcctgc tgtatgccgc cgatctccac catttccatg cggtcgattc
     7261 caggagcttc tattttatgg cttgtacgta taatcgtggg ggctatcaga cgcaaaccgg
     7321 caaacgccag aacaaaaaca gcaactagta tgcaaagtgt gattaatatc attttaagta
     7381 ccttctttca tgaatttttt tagccataga tatctcctct aacattaaaa aaaatatgtt
     7441 gctattttag ttttgttact ctacctgtcc cattggtttc ctttcataaa aaatattaat
     7501 tgattctata atctcatctt ccaggatatc aactcctatt tgtctcataa gaaactgtaa
     7561 gaattgaaac cgtttacgaa gtgtaggaaa gtccccctga aatacatctg tatcacacca
     7621 aaagttaaag agcagcaaaa ttacctctgc gcattcatct gggaactctg tgatgatgct
     7681 tctatcctct atgccttctc gtaaaacatt tgctaatatt ggtgctagtt ttttcaaatt
     7741 atcctgtgta aatgccagta tgatatgcgg actttctatc gcgcttgtaa tcatgttact
     7801 agattctttt atcacctcat ccgttaaatg ccgcctaact aaacttctaa gcttctcttt
     7861 tgctgtaagt ccatgcattt caccgagcca ttgatttact gtctctatta tctgttcaaa
     7921 gcgcctttcc atgaccacat taaaaatatc ttcttttgaa ctgaagtgat gaaaaattcc
     7981 gccctttgac attcctgaag catcaacaat attttgcata cttgttttgt catatccttt
     8041 ttcagcaaac aactttgcag aaatggtaat gatattttca atagcttgct ttggattgtt
     8101 ctttctagtc attatataac tccttcttat taaaaaccga ctgttggttt ttattatata
     8161 agctattcct gcttctgtca agtaacattg tgtttatggc aaaacaataa ggtttggtgg
     8221 ttagacaggt attaattaga ctaatgcgac tttcccctag atggaaccct tggtaaattt
     8281 gcaatcttta ccggctttga ttatatctgt aagcaaggca ggtatggcca cacctgccct
     8341 gtttgcccct cggatattca ttcctatccc tcctctcctt agccattaca ccaaaacgcc
     8401 gctcattgtc tccaatggcg gcgttttaaa gatatgattt ctcatgctgc tgaaatatgc
     8461 aaaaaatata agatgccctt tattattttt cctacaatag aaaagaaaag tattgaagaa
     8521 tgggttaata ttggagcaaa tatgctttgt ttggcacaag atactacctt tatcacttat
     8581 gttatcaatc aattattatc tttttataat gagattgtac caattcaaaa acaacattaa
     8641 attataaatc taattttata ttaaaagaga ctaatatttt atctatctct attaaggata
     8701 ttttcttatg tatcataata ttagtctctc ttattattat atactgctat catcattagt
     8761 tgctatttat actattttta taatatacaa atttattcag ctaacgtttt gcctaattct
     8821 atacactcat cttttgcttc ttcatttggg tcttccattg ctgtaattcc ttctccacca
     8881 ataattgtag caccagcatt ttgcatacgt tcttcccagt ctctcatcca ttcacaattt
     8941 ccccatccat atgaaccaaa taatccaatc tgtttacctg ataccataga ttctaattct
     9001 gaaacaaatg gctccatata cccctcttct agttgctctg ctcccataga tgggcaacct
     9061 aatgcaaatt tatcttcttc ttttaaatca cttggtttca gtaagctcac atctaaaact
     9121 tctggtgtct taccttttaa cttcacacct tcagccacaa aatttgccat tttttcagta
     9181 tttcctgttc cactccaata aacaatatat attttactca tttttacctc ctgtagtatt
     9241 ttttaattaa tcaatagtta gttatagcta actaacatct aaaaaacaaa agatttcttt
     9301 atctgaaatc tctttatttt tctaaatata gaatatcatg gttgattttg aaattcaata
     9361 cttattgata atatttatca tttatatttt atattcattt tataatatca aaacactgta
     9421 tcgtatttct aatctttcat agaaaaattt ctaacttata gtacttttgc atctcttata
     9481 aatcttttat atgtataagt acatagaaaa acacaccttt tccagatgtg ctatctataa
     9541 tatcgtatat ttatttttca attctaacta gctaatatgt ttataaattt tatcacttaa
     9601 tacctttata cattgatgta aaatcttaca cctaagtaat ttttattatt tgtctcgact
     9661 ctatataata tagctatttt atcatcaaat caaaaattat cttttgctat tattcttgta
     9721 tttataaaga tttaaatata agtatccatt tccttgttcc gttctaggta ttccaagcgc
     9781 tctcgtacat tttattaatt gtgcatacag ttcagcttca tctaaatctc tttcaaactc
     9841 ctctcttgcc cattctttaa ttagtgctaa tgagcctgat acgtatggtg cactcatact
     9901 ggtaccactc ataatagcaa gtttattatc catataagtc gatataatat ttcttcctgg
     9961 agctaccaaa tctattgtag tatttgaatt actaaacttt tcaaccaaat agttctcatt
    10021 tattgcacct acttctatta cctcagcata actggctgga taactatact cacttgtact
    10081 agaatcacca tctccattat tacctgctgc acacactaca gaaatattat tttttactgc
    10141 ttgcatgaca gcatttttta aattcttatc atctttgttt cccccaagag acatagatat
    10201 aatatcaacc ttgttattta cagcaaagtt aatagcatta attatacttt gatatgttcc
    10261 agtaccatct ttatttaatg cttttgctat taataattta caatctggag ctactcccat
    10321 aacttcatta ttataattag atgcagctat aatacccgcc acatgagttc catgaccatt
    10381 aaaatcctca tatatatttt tatttccatt actgtcatca ctaaaatttg caccaccaat
    10441 tatttttcct tttaaaagag gatgagatat atcacaacct gtatctatta taccaactac
    10501 aatattttta ccagtataac cttcatccca cattccccta gcattcattt gttttattcc
    10561 atatgggaat ttattttttg cacctcttag attttcattt atttcatatg gaattagttt
    10621 cattctttta ttcatcatat atcctcctaa aaattttatt gcttataaaa atcatgaatt
    10681 ttattttata ataggtttat aaatcaaatt aaaacc
//
LOCUS       DAMJZE010000135        10703 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJZE010000135 DAMJZE010000000
VERSION     DAMJZE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.138"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            280..738
                     /locus_tag="PC541_002845"
     CDS             280..738
                     /locus_tag="PC541_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2840292.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            799..1074
                     /locus_tag="PC541_002846"
     CDS             799..1074
                     /locus_tag="PC541_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2840293.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1133..2407
                     /locus_tag="PC541_002847"
     CDS             1133..2407
                     /locus_tag="PC541_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2840294.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2537..3589
                     /locus_tag="PC541_002848"
     CDS             2537..3589
                     /locus_tag="PC541_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2840295.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3589..4620
                     /locus_tag="PC541_002849"
     CDS             3589..4620
                     /locus_tag="PC541_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2840296.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4712..5524
                     /locus_tag="PC541_002850"
     CDS             4712..5524
                     /locus_tag="PC541_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2840297.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5527..6447
                     /locus_tag="PC541_002851"
     CDS             5527..6447
                     /locus_tag="PC541_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2840298.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6622..7068
                     /gene="rpiB"
                     /locus_tag="PC541_002852"
     CDS             6622..7068
                     /gene="rpiB"
                     /locus_tag="PC541_002852"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2840299.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7098..7763
                     /locus_tag="PC541_002853"
     CDS             7098..7763
                     /locus_tag="PC541_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2840300.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7873..9375
                     /locus_tag="PC541_002854"
     CDS             7873..9375
                     /locus_tag="PC541_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2840301.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9687..>10703)
                     /locus_tag="PC541_002855"
     CDS             complement(9687..>10703)
                     /locus_tag="PC541_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2840302.1"
                     /translation="NKKLAIVFLISIVVQAIIQYLVLIFFMYIYYAEAIAIVILFTLV
                     NTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITG
                     GLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINIL
                     DKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFII
                     ETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISND
                     KLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTF
                     RRVN"
BASE COUNT         4268 a         1218 c         1969 g         3248 t
ORIGIN      
        1 aaccatttgt aaaaaacaaa aaaatcattt ctctaacaaa aaaaaccaca aaaaatataa
       61 aaaagtatgg ctagttaagt gattaacaag taaaaattaa aataaaaatc aaaaaaaatt
      121 tatcaatgaa agtcagctaa aatcctggaa gagagcatta tttttgggaa aacattttct
      181 tttgtttcac aaaaaaagcc actttttggc acacaacatg ctttatataa agtcaagaaa
      241 aactaaggta taagacaaag gcaattagga ggtgtaaata tggagtttat aaattttgta
      301 agtgataaag ttatagacct taaaatggat tgtaaaacga aagaagattt ctttaaagaa
      361 atacataata aagtttttga tttaggcttt gtaaaagaag aatttggaga aaaaatatta
      421 gcaagagaaa atgtatttcc aacaggttta aatttaggtg attatggagt agcaatacca
      481 catacagatg cggaatatat taaagagcaa tttatatcag tctgtacttt taatgaacca
      541 gtagtattta gttctatgga agaccaagat gaaaaagtac ctgtaaattt ggctttcgta
      601 ttaggactta atcaaccaca cagtcaatta tctgtattaa cagaattaat gggaattatg
      661 caaaataaag agttagttga taagttgatg agttctactg ataaagaaga agttttaaag
      721 acactaaagt ctttataaaa ataaaaaagt actaaaaata aaaaatataa aaataaaaat
      781 tattaggagg aaataaaaat gaaaaaaata ttagtagcat gtggagcagg aatagcaaca
      841 tcaacagtag tttgtgacaa ggtagagaga ttagttaagg aaaataatat acaagcagaa
      901 gttatacaat gcaaaatagc tgaatgtgca acaaagcaag aaggagcaga tttaatagta
      961 tcaacaacta tactaccaac aacttataat ataccagcaa taaaagcaac agcatatata
     1021 actggagtaa atacagcagc attagataaa aaaatattag atgctttaaa ataatcctat
     1081 catagataca ttgaaataat attttataaa acagaaaaag gaggatttat aaatggataa
     1141 attattagca gtagtacaat acattttaaa tatgggaccg acagtaatac taccaataac
     1201 aattgtagta ctaggaatga tatttagaat taagtttaca aaagctttaa agtcaggtct
     1261 tacaattggt ataggatttg tgggtataaa cttagttgta aacctattaa catcaacact
     1321 agggccagcg gctcaaggaa tggttgaaag attcggactt aatttaacag taatagatgt
     1381 tggttggcca acaacagcat cagcaacatg ggcttcacca gttgcaccaa tattaatacc
     1441 agtttgttta ttagtaaact tactattatt atacttcaat aaaacaaaga tattaaacgt
     1501 tgatatatgg aattattggc actttatagt agcaggtgca gttggatata tagtaacagg
     1561 aagttatgtt tgggctatat tctgtgcaat actaatggag ttattagtac tgtttattgc
     1621 agaaaagaca gcaaaaaatg ttcaggagtt ttatgattta gaaggtatag cattaccaac
     1681 tggttcaaca gcttcatttg ctccacttgg atatttagta ggtaaatttg ttgaaaaaat
     1741 accaggaata aataaaatac aagcagaccc agactctata caaaaaagat ttggtatatt
     1801 tggagaacca atgatgatgg ggattatact aggtataata cttggagcac ttgcgggata
     1861 tgatgtagca ggaatattca atgtaggtat atcaatgggt gcagtaatgt tacttatgcc
     1921 tagaatggtt agaatattaa tggaaggttt aattccaatt tcagaatcaa taagagattt
     1981 cttacaagca aaatatggag acagagaact ttatataggt ctggatgcag cagtagcaac
     2041 tggacatcca gcagttatat caacagctct tatacttgtt cctataactt tgttcttagc
     2101 agttatacta ccaggaaaca aagttttacc atttggagac ttagcgacaa taccatttta
     2161 tatggcattt atagtgtgtt ttagaaaagg taatatagtt caatcagtta taacaggaac
     2221 aatagtaata gctatatcac tttatatggc tacaaacttt gcagatgttc atacacaatt
     2281 attacaacaa gcaaatatga tgccagaagg aacaacaatg gttacaagta tagatacagg
     2341 tggaaacttg ctaaaatggg gaatattgaa attatctcaa ttagtttctt catttggaat
     2401 attctagtat gtagttaata gagattactc ttttttagta aagtatgcga aaatttaaca
     2461 tttagcagct actaaaaaag agtaatattt tttataaatt tttaaaaata caaaatacaa
     2521 gaggaggaga tttatcgtga aagcagcagt attacatggc acaaatgata tgagatttga
     2581 agatatagaa ataaaaccat gtgaaagtga tgaagtaaaa ataaaagtta tggcagcagg
     2641 aatatgtggg tcagatcctc caagagtatt aaaacattgg aaatatccag ttccagctat
     2701 accaggacat gagttttctg gagtaattgc tgaagttggt aaggatgtaa aaaatgtaaa
     2761 agttggagat agagtagttg caattccttt tataccttgt aatgaatgtg aatattgtaa
     2821 aagaggatta ttttcattgt gtgatgacca tggaatgtta ggagctaaaa gttttggagc
     2881 ttttgcagag tatgtaaata taaaagctac aaatgtactt ccaataggag atatgaactt
     2941 tgaagatgct gcaatgatag agccactagc agttgctatg catggagtac ttaatatagg
     3001 tgttcaagtt ggagacactg tagctgtaat gggtagtgga acaatggggc agttggtaat
     3061 acaaggactt aaaatagcag gggcaggaac aattatagca gttgatatat ctgataataa
     3121 attaagagaa tcaaaggaat taggggctga tataataatt aatgctaaag atattaaccc
     3181 agtagaaaaa ataaaagaac ttactggtgg aaaaggtgtt gacatagctc ttgaatgtgc
     3241 aggttcaaaa ataactcaag aacaatgttt acttataact aaaaagaaaa gtaagatagg
     3301 atttttaggg atagcatatt cagatataac tttatcagaa gaagcatttg aaaatatatt
     3361 tagaaaagaa ctagaattaa aaggtttttg gaactcttat tctgcaccat ttccaggaca
     3421 agaatggact aagggaatca atctagttaa tgaaggaaag ataaaattaa aagaaatggt
     3481 atctcataga ttctcacttg aagatactta caaagcattt gaaatgataa gagatagaaa
     3541 agaagaattt aacaaaatat taattttacc acaaggggtt gaaaaataat gaaagcagta
     3601 gttaaaacta aaccaggcta tgataatgta gaagttttag aagtagaaga accaaaagct
     3661 acaggagaca aggttaaaat aaaagtagaa tatagtggta tatgtggttc agatatacat
     3721 tcatttaaag gtgaatatgc aaatataaaa gccccagtaa cattaggtca tgaattttct
     3781 ggaattgttg tagaagtagg agaagatgtt aaaaatataa aagtaggaga tagagttaca
     3841 tcagagacta cttttgcaac ttgtgagaaa tgtatatatt gtgaaactaa agattataac
     3901 ttatgcccaa ctagaaaagg tattggtact caagtaaatg gaagttttgc tgagtatgta
     3961 ttatcaagag aagaaagcat acatgtactt cctgaaaatg tatctttatt atcagcagcc
     4021 cttacagagc cactagcttg ttgtgttcat gcagctcttg aaaagactac tatagaaagt
     4081 tctgatacag ttttaataat aggaccagga ccaattggtt tattattagc tcaagttgtt
     4141 aaatctcaag gtgctacagt tataatgtct ggtgttacaa aagataagga aagattagaa
     4201 atagctaaaa atcttggagt agatagaact gtaaatgtaa tggaagaaag tttggcagat
     4261 gtagtgaatg aaatgactga tggaaatgga gtaaacaaag gatttgattg ttctgggttt
     4321 atgccagcag ttaacgaagc cttaagactg cttagaaaaa aaggtacttt cgtacaagtt
     4381 ggaatctttg ctaaaaaatt aaatgaaatg gaccaagaag caataatcca aagagagtta
     4441 caatatatag gaagcaggtc tcaaaagcca agttcttgga ttacagcatt agatttaatg
     4501 gcaaatggga aagtaaatac agaagcttta gttactagaa ctgttggttt agacgaattt
     4561 aaagaaggta ttacagcttt aatgaacggg gaagaaataa aagtagcagt aaaatcttaa
     4621 aaaaacattt gtattatttt aaaaaaaatt gtaaaatcta ctgtaggata aaaaaatgta
     4681 aggtcttaaa aaataacagg aggaagaaag tatgagcact cttagagatc attctaataa
     4741 tattagaaaa aacataataa aaatggttgc agaagctaaa agcggacatc caggtggttc
     4801 attatcaata gctgatatgt tgacagtatt atattttgat aagatgaacg tatgtgcaga
     4861 aaatccaaaa atggctgata gagatagatt tgttctatca aaaggtcatg cagcaccagc
     4921 tttatatgct acattagcag aaaaaggatt tttcccagaa gaagaattaa taacactaag
     4981 aaaatttggt tctaagttac aaggtcaccc agacatgaaa aaagtagcag gaatcgatat
     5041 gtctactggt tctcttggtc aagggctatc tgcagcaaat ggtatggcac ttgcaggaaa
     5101 attagataac aaagactata cagtatatac tatattaggt gatggagaaa tccaagaagg
     5161 tcaaatatgg gaagctgcaa tgacagcatc acattacaaa ttagataatt taattgcatt
     5221 tgttgactta aatggattac aaatagatgg ttctaatgaa gaagtaatga atgtaagtcc
     5281 agtagatgac aaattcaaat cttttggatg gaatgtaata gtaataaatg gacattcttt
     5341 tgaagaaata ggaaatgcag ttgatgaagc taaaaaagtt acaggaaaac caactgtaat
     5401 aatagctaaa acagtaaaag gtaaaggtgt ttcatttatg gaaaataatg ctgcatggca
     5461 tggaacagct ccaaatgcag aacaagcaga gcaagcattg aaagatttag aaggaggtct
     5521 ataggaatgg gaatagcaac tagagaagct tacggacaag tgttaaaaga acttgcagaa
     5581 aataaagata tagtagtatt agatgcagat ttaggaaaag ctacaaaaag tatatcattt
     5641 aaagaagttg ccccagatag attttttgac atggggatag cagaaggtga catgattggt
     5701 acagcagcag gtcttgctac ttgtggaaaa ataccttttg cgagtacttt tgcaatattt
     5761 gcagcaggaa gaggctatga gcaaataaga aactctgtag catatccaaa tttaaatgta
     5821 aaaatagcag ctactcatgc aggtgtaaca gtaggtgaag atggtggtag ccatcaagct
     5881 atagaggata tatctctaat gagaggtata ccaaatatgg ttgtattaaa tccagcagat
     5941 gctttagaag caagacaagc tattcttaca tctattgatt ataatggacc tgtttatata
     6001 agattaggta gagctgcaac tcctgatgta aacagtgaaa attataaatt tgaaatagga
     6061 aaaggaactg ttttaagaga aggttctgac ataacagtaa tagctacagg aataatggta
     6121 gcaaaagcct tagaagcagc agaagaactt gctaaagaag gtataaatgt agaagttgtt
     6181 aatatatcta caataaaacc attagatgaa gcattaataa aagaaactgc taagaaaact
     6241 ggaaaagtag ttacagcaga ggagcacagt ataataggtg gtttaggttc agctgtatgt
     6301 gaagctttag cagaaactaa agatgtagta gttagaagaa ttggtgttaa agatgtattt
     6361 ggtcaatcag gtacaccagc tgatttatta aaacactatg gtttaactac agaagatata
     6421 gtaaaaaata taaaagagtt gttatagata taaatatatc taaataacaa attgatttga
     6481 cataagtcaa ttaaagctgt cttaaaaata gaaagaaatt tctacaatgg gacagcttta
     6541 tttttacaat actttatttt tatgataatt ataaaatagt ctgaaagtaa tcaaaaaaat
     6601 tataaaaaga ggtgctattt aatgaaaata tctattggat gtgaccatgg tggttatgaa
     6661 ttaaaggaat atataaagga aaatttaata aataaaggta tagaagttga agatgtgggt
     6721 acaaattcaa aagaaagtgt agattttcca atatatgcaa aaaaggtaag tcaaaatgtt
     6781 caaagtaaaa aatctgattt aggcatactt tgttgtggaa caggaatagg tatgtctatt
     6841 gcagcaaata agtttaaagg aatcagagca gctgttgttt ctgactgttt ttcagctaag
     6901 gcaactagag aacacaataa tacaaatgta ctttgtttag gagaaagagt tattgggaaa
     6961 ggattagcaa tgagaattgt tgaagaatgg ttaaattcaa gttataatgg agaaagacat
     7021 gcaagaagat tacaaatgat tgatgatata gaaaaagaaa atttataaat atataattta
     7081 taacaagggg gagcgttatg tctattattt gtgcttcaat aatgtgtgca gaccaaatga
     7141 agttaaaaga ggaattagaa gctcttgaag aagcaggtgt aaaattactt cattgtgatg
     7201 ttatggatgg aatatttgtt aaaaacttgg ctatgggacc agagttatta aaatcaataa
     7261 gtgaaaatac aacaatacct ttagacatac acttagccac agaaactcca gataaatata
     7321 ttgatatgat gtcttatata aagccaaaat atatttcttt tcatgtagaa tcaagtacaa
     7381 atgtaaaagc tgatatacaa aagttaagaa attatggtat agggccagtg ttagcaataa
     7441 gccctcaaac aagtgtggat aaaatagaag aatatataag tttagttgag ggtatcttag
     7501 taatgacagt aaatccaggg tttgcaggtc aaaagtttaa tctttctgta ttggataagt
     7561 tggataaact tacagagata ctaaaagatt atgataatcc tccatttata gaggttgatg
     7621 gaaatataaa taaggatact attaaactta tgaatggtaa aaaagtagat atatatgttg
     7681 ttggtacttc agctttattt aatgataagc ctccaatttc atataaagat aaaatagaag
     7741 aactaaaaga aagtataaag taaccatgta ataaaattat aatatgtttc tgtttaatag
     7801 taaaaattta ggtggaaaca agaaatttta taatatttaa taaaaatttt ggttgagtaa
     7861 ggagaatttt ttatgggaga cctatataaa ctacaaaatg gtactgatat tagaggaata
     7921 gcgtatgaaa atgattctaa agaagttaac ctaactgtag aagaagtaaa aaaaatagct
     7981 aaagcgtttc atatttggtt aaaagaaaag actaagaaag ataaagtaac tgttgccata
     8041 ggaactgatt ctaggataac aggaagccaa tttagaagta ctgttattga aactttaact
     8101 aatgacgatt gtaatgtaat tgattgtgag attgccacaa ctccagctat gtttatgaca
     8161 actattatgg atggatacaa ttctgatggg gcgattatga ttacagcaag tcacttacct
     8221 tattactata atggtttaaa gtttttcaca gaaagtggag gtttagaaaa aactgatata
     8281 aaagagatgt tggatatagc tgttaaaaat gatagtgtag actatgagga agtaaataaa
     8341 aaaggtgaag ttatagtaaa aaatttaata gaagattatt ctaatttatt gatagataaa
     8401 attagaaaag gtgtaaattc aagtaaaaat tatgaaaagc ctttttctgg gcttaagata
     8461 ttggttgatg caggaaatgg ggctggtgga ttctttgctg aaaaagtttt acacatactg
     8521 ggagcagata cgacaggtag tcagtttttg aacccagatg gtatgttccc aaatcatata
     8581 cctaatcccg aaaacaaaga agcaatggaa agcatatgta aagctgtgtt agataataag
     8641 agcgacctag gaataatctt tgatacagat gtagacagag ctgctattgt aggtaaaaat
     8701 ggcaaaccaa taaataaaaa tgctcttata gctgttatat catcaatagt attagaagaa
     8761 catccaaaaa cagctatagt aacagattcg attacttctg aaggacttgc taaatttata
     8821 aatgaattac aaggaagaca tcatagattt aaaagaggat ataaaaatgt aataaatgaa
     8881 gctataagat tgaatagtga aggtgaagag tgccatttag ctatagaaac gtctggacat
     8941 gctgctttaa aagaaaatta tttcttagat gatggggcat atttaatagc taaaatttta
     9001 ataaaagtag caaagctatc cttggaagga aagactatag aagaattgat agaaaacctt
     9061 gaagaagctc aagaagagaa agaagttaga ataggcatta ataaagaaga ttttagacca
     9121 tatgcagagg atatattaaa agatttagat tctcatgtca agagtattga tggatggtct
     9181 gtagcaccaa ataattttga aggcataaga gttaattgtg atgaatcaaa tggagatgga
     9241 tggtttttac ttagaatatc actacatgaa ccattattag ctttaaatat tgaatcaaat
     9301 aaaattggcg gagcagaaca aatttattct aagttaaaac ttttcttaga aaaatatgat
     9361 ttaaaaggtc tttagggtaa atagaaagaa tatatttata ttgaagaata aatgtaaatt
     9421 tgtacctaag attacaaata ttacaaaatt gtaatcttag gtaacagaga tgtaactgat
     9481 tatggaaaca ctatgtgata ttatagatac agagagatga aatatgtgat gaattataga
     9541 gaaatataat ttaaaatgta ttaatatcac accaaaaagg tataatcaaa aaatatgtaa
     9601 atataaaccc caacatttag aagaagacct ctaaggaggt ttttttcttg tttaaataac
     9661 ttaaaatata tttttatatc aatatatcaa ttaactcttc taaaagtaag cattacctta
     9721 aataaatcag catctataat tatttccatg tgtgcatttt ctaattcaat tatattttga
     9781 gcaattgata aaccaagacc agagccatct gtttttctag atatatcacc ccttctaaat
     9841 ctttctatta actcttctgg ctttagattt agtttatcat ttgaaatatt tttaaacgtt
     9901 aatattatat tttcttcctt tataaacata tctatgtaga ccctagtgtt aggcatagag
     9961 tattttagta tattgcaaat tagattctca aaaactctcc aagttttttt accatctatc
    10021 atggtaaaaa cagcatattc aggtgtttct attataaaat ctaaactgct attttgaatt
    10081 ttgtcttcaa actctacaat actttgaatt aaaagttctc ttatatcggt ttttgttata
    10141 ttaaattcta catctccact acttagctta gatgcttcaa atatgtcatc tattaataat
    10201 tttaatcttt gtgatttttt atctaaaata tttatatagt catttatagt ctctctgtca
    10261 aagtcattat tttttaacat atcaacatag cttaatatag atgtaagtgg agtttttaag
    10321 tcatgagata cgtttgtgat aagctctgcc ttcatccttt cattttttat attagcatct
    10381 acagcagttt caagaccacc tgttatatta tttatacttt tggctaaagt agatatttca
    10441 tcatctccta taacttctaa tttatacttt aaattgccat tttctatatt ttttatattt
    10501 tttgatatgt tatttatgta attaaactta acagataaaa agttgtatat ataaaaatga
    10561 aaaactgtgt ttacaagtgt aaataaaatt actatagcta ttgcctcagc ataatatata
    10621 tacataaaga atatcaaaac aagatattgg ataatagctt gtacaactat agatattaaa
    10681 aatactatag ctaatttttt att
//
LOCUS       DAMJZE010000136        10677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJZE010000136 DAMJZE010000000
VERSION     DAMJZE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.139"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            99..446
                     /locus_tag="PC541_002856"
     CDS             99..446
                     /locus_tag="PC541_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2840303.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(596..1096)
                     /locus_tag="PC541_002857"
                     /pseudo
     CDS             complement(596..1096)
                     /locus_tag="PC541_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1123..2628)
                     /locus_tag="PC541_002858"
     CDS             complement(1123..2628)
                     /locus_tag="PC541_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2840304.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2827..3681)
                     /locus_tag="PC541_002859"
     CDS             complement(2827..3681)
                     /locus_tag="PC541_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2840305.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4066..4347
                     /locus_tag="PC541_002860"
     CDS             4066..4347
                     /locus_tag="PC541_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840306.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4497..5345
                     /locus_tag="PC541_002861"
     CDS             4497..5345
                     /locus_tag="PC541_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDF2840307.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5397..5918
                     /locus_tag="PC541_002862"
     CDS             5397..5918
                     /locus_tag="PC541_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840308.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            6269..6802
                     /locus_tag="PC541_002863"
     CDS             6269..6802
                     /locus_tag="PC541_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDF2840309.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            6799..7560
                     /locus_tag="PC541_002864"
     CDS             6799..7560
                     /locus_tag="PC541_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840310.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            7598..8464
                     /locus_tag="PC541_002865"
     CDS             7598..8464
                     /locus_tag="PC541_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840311.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            8485..9726
                     /locus_tag="PC541_002866"
     CDS             8485..9726
                     /locus_tag="PC541_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840312.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(9950..10522)
                     /locus_tag="PC541_002867"
     CDS             complement(9950..10522)
                     /locus_tag="PC541_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2840313.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT         3943 a         1368 c         1466 g         3900 t
ORIGIN      
        1 ataaaagaat tttattccta ttgtgtaaag tgagaatact attttcttat attggtataa
       61 taatagtaaa tctaatatag aaaagaggta tttctagtat gcatgaaaat aaaaaaaatc
      121 tattagacgg aagcttagct caatataatg atacagcagt tccaaaaata ccagtatttg
      181 ctggtaacga tataacttca gaagtttatt attttaaacc taatcaagtt cttaatgctc
      241 atagacaccc taatggtgaa caaatatttg tgttcctaaa aggagaaggt aaaatgaaac
      301 ttggtgagca tgaatgtgat gtaaaaaacg gcgatactgt atttgtacca acaggagagt
      361 ggcatgaaat tactaatgga agcaatgaag aaatggtagc cgttcaaatt acaaaaatta
      421 atgctggagc agaatacaga ggatagatat ataagtacag ttagtacttt aatatctatt
      481 ttattatata aactttaatc tcaaatataa aaagagtctt ttaaaaggct ctttttatat
      541 ttttgttaat tttcattata aaaacaagaa aatatctagc taaaactaat atattttatt
      601 taatattttt tatagcattg tttgcaaact cagtatttac gatatcttta taaggtgctt
      661 ccttatctag ttctttagct tctttcataa cagtcatcaa tttcttcaaa ccttcctctt
      721 ttaatacagg attttgagac catgcatcta ttgatttata tctatctaca actgttgtta
      781 aatcttctaa agatatatct tcaaagaaag gttgcatagc cttagctact tctttgctag
      841 atgcttgttg aatccataat tgacctttat aaacagcatt tgtaaatctt tgaattaaat
      901 ctttattgtt tttcatgtat gatctcgtag tagagtatgc tgtaaaaggt atttctccag
      961 aatcttttcc tatagatgta actatatatc cactaccttc tttttccatt gctgttgctg
     1021 taggttcaaa tgctgttgta aagtcaccct ctccacctgc aaaagctcca gccattacac
     1081 caaattggta atcagtctat ttataacatt taaaacatat aattaatctc ttgggatatt
     1141 agggtaaagt attaaagaaa aatcatcaga tttttgatta ggttcttttt tatagactgc
     1201 gtaatttatt acacttttaa gcaatatatt ttttagataa ggttcttttt cctctgtata
     1261 ttcacttaaa attttttcaa gttgaggaac aatttgtgtt ttaactgtat ttctttcctt
     1321 ttgcatttca atatctatat ttgttttatt aatagcttct cttgcatttt ctatacgaga
     1381 taagagtatt tttaaacgtt ccccaaatgt attactgtca taaattcctt gttctaaaaa
     1441 atcatgtagt cgttcttttt gtttttctaa agtatccaat tctttattta aatttatcaa
     1501 ggtatgttct aaagtatttt caggtttaga gttgtctttt tcatctttta aagatagtct
     1561 ataatttact aaccatttat ttaagacctc taatatttta ttttctaata tgtatagttt
     1621 agtagcttta tttttacaat tagtacaaac catatatttc tcatatccat ttgatgtagt
     1681 attagatttg gctattaaag tatgattaca gtatccacat tttacaattc cagccaatgg
     1741 atttgttatt ttattgtttg gagtagggga attaatacgt ttttttctta tattttgtgc
     1801 cttattccat atgtcttcac ttattattgg ttcatgcttt ccttttacct caatttgttc
     1861 ctcaattcct cttgttctac tagaatttct tttcctctcg actttattcc atactatata
     1921 tccacaatac actttgtttt ttataatatc tcttataccc ttttcatacc aagttctacc
     1981 tgttgtggtc ttataaccaa gttcgtttaa ataattggat attttacatg caccatctcc
     2041 ttttaaatac atatcaaaga ttattttaac aacagttgct ttatcttcat ttattacaag
     2101 tactctttct ttattattta aatatttaat atcataccca taaggaggag cttgagctat
     2161 aaatttaccc tcttcaatac ttttgatttt tcctctttgc atacgtctag atattaattt
     2221 taattcctta cgagccataa aagcttcaaa ttcagaatac tcctcatcaa actcatcatt
     2281 taaatcatac gtttttctag gagtaataat tttcgtttta gattttttaa atgtatctag
     2341 tattaaccct tggtcttgca tattaccacg acccaaacgg tcaatatcca tcactaaaac
     2401 agcatcatag ttattatttt taatttcacc tagtaattct agcatcttag gtctatattc
     2461 tatactttcc ccagaaacaa gttcttcttt aatttcaatg acatttaaat tttgttctct
     2521 tgctagtttt aaaagagttg tcttatgtct acttagagtt tcaaattcac ctctatgttc
     2581 tgcttcttca tcagctctgc ttttgcgtaa ataaattgcc acattcatta aatcacaccc
     2641 cttgaatata ttatattcag taacagattg attatattta aaacacaaaa ataaaccttc
     2701 aaaatacaag atatattttt taggtttatt tttatacagt aaattttata gtatatttaa
     2761 aagcttgtaa tctaagtaaa tatttatact attaatttta tgctttcttc attttttaat
     2821 tttaaactat ttttttacta caggtatcca tacttcaaat ttagcatttt gagggtctgg
     2881 atttaagtac agttcaatat caggaccatt atcatattca taaccagaag taggtaacca
     2941 ttctgttaca atacgctttt ctagttcttg tattgcttgt ggacattgtc cttctccaga
     3001 aaatattgcc caagtaaaag ctggaacagt gtattcttct aatgtattat ctatagattt
     3061 tgtacttgaa acagatataa aatacttcca ttcttctgaa ttattacaaa tacttatacc
     3121 taatacacct tgaagttgac tatccatcat agggattaat ttttgaagcg ttccatctaa
     3181 agcaaccttt tgccacattt ggggaacaat ttcaaaattc ttttctagtt cattatgaag
     3241 tggctgagat acaccaacta tacgaaatgc ttctttcttt tcaattctat aattcatttc
     3301 ttcggctcct ttaatactaa ttttaaaact gattggagga aaggctttta aagttgttat
     3361 ttctcttttt tttataactg atggggcaac accatgaata cttttaaatg cacgattgaa
     3421 tgctgttgga gaagagtatc catattttaa agccacatca attatctttt tatcatcatt
     3481 ttgtaattct acagctgcta aagacatccg tctacgacga atatattcag aaagtggtac
     3541 atctgccata taagcaaaca ttctttgaaa atgatatgta gaacaacaag ctatttttgc
     3601 cacttgttca tactcaattt cttttgttat gttttcttca atatagttaa ttgctttgtt
     3661 taatctttca atccattcca taaaatcagc tccttgtaat ttatataata tctattttaa
     3721 aatagttaat cctctctttc tatgcactaa ttaactagct taataaagaa tgaacatgag
     3781 aaagtctgta attaactaca aaaatgaaca aatagtattg tttttcacat taaagtatat
     3841 cacaaattag tatctaattt ttatataaga taacagtttt aatttattat gaaattttta
     3901 taatatttta gcttagacat ttaattatat ataacatttt atttatagtt aactattttt
     3961 gaagtttacc cttaatcaag caacacttag ttattcaatt ataaataatg attgagaata
     4021 ggacaatttt taagatacat aaaatataaa gaggagggga tttaaatgcc aaacatagaa
     4081 aaagacttag atggtacaac tactataaca tacctcaacg acgatatagg tgttccaaca
     4141 gacgagaatg gatataaaat atctatgaca atactacatg gaaagacaat ctgccatgta
     4201 gtatctcctg aaacaatctt aggaagaaag ttcacattag aagaaaaaca agaaaaagat
     4261 acaataatat atggagcaat agaaaaaata atggtagaca gacaattaca aataaaaaat
     4321 tcaaacaaag aagttgctag tcattagcac ttctttaata agtaaagtag gacaagtatt
     4381 ttttataata ttattgactt atagtctaat tagactataa tataaacata gatagtctaa
     4441 ttagactact attaaaaata aatacaataa gaatttaaga aaggataata ataataatga
     4501 ttaaatcatt tttaatgtta ggacagtcca atatggctgg aagaggattc ataagtgaag
     4561 tgactccaat ttataatgaa agaatacaga tgttacgtaa tggaagatgg caaatgatga
     4621 cagaacctat taattatgac agaccagttt ctggggtaag tcttgctgca tcatttgcag
     4681 atgcatggtg ctgtgaaaat caagaggata gaattgggct aattccttgt gctgagggag
     4741 gcagttcctt agatgaatgg aatatagatg gtatactttt taaacatgct ataagtgaag
     4801 ctaaatttgc catacaaagc agtgaattga caggaatttt atggcatcag ggagaaaatg
     4861 acagtaataa tggcaactat aaattttatt ataaaaaatt actttctatt attgaaactc
     4921 ttagaaaaga attaaatatt ccagatatac caattattat tggtggattg ggagacttct
     4981 taggaaaagt aggttttgga aaaagttgta ctgagtatgt atttatcaat caagagttac
     5041 agaaatttgc ttttgaacaa gataattgtt actttgttac agcaacaggt cttacatcta
     5101 atcctgatgg tattcatatt gatgcaatat ctcaaagaaa atttggatta cgttattttg
     5161 aagcttttca taagaaaaag catattatgg aagcattagc taatgaaagt gagttgataa
     5221 taccgagtaa ttccaagaca tatacaaaaa cagaaaaaat atatataaat agtatggact
     5281 tggcactagg aaagatatca tatgatgagt ttgaatctaa acttattaaa cttaatgata
     5341 attagataaa atattcatta atatactaat aaatatagat tgaaagaggt gattttttga
     5401 taccattatt aattttagga ttattaaaac aaaatccaaa ttcttatgga tatgagttat
     5461 tagcattaat ggaaaagaag cattacaagt acatagtaaa tttcactaaa ggttcttttt
     5521 attataattt acagcaactt gaagaaaaaa agtttattga aaaagttgaa aataaacaat
     5581 ctaataaaaa tagagaaact cataactaca taattactga acttggatgc caggaatttg
     5641 aaaaattaat gtataagtat ggttctaaaa cagagtatat aaatctatca ttttacgcaa
     5701 cactattatt tgcaaatgat tataaaaatg aaaagttaat agaattattg gaaacgcaaa
     5761 ttaagcaaac tcaagtaaga atttctctat taaatgaatc catagaaaat gacaaaacaa
     5821 attcacatta ttttaataga atgctagaaa attctcgttc tcaccattta gttaatattg
     5881 aatggtttga gaagttgcta aatgatatta gagattgatt ttaataataa aaaaacatac
     5941 atatatattt tatataaatg tatgatatat agattaaaaa taaggcagat aattttcaat
     6001 tgaatatctg tctttttgtt atataagatg ttaaaagaaa cttatactat gtaagtaaaa
     6061 tttataaata taaagttgtt cattgttgaa ttacagacac ttaagatgaa aagtatgaca
     6121 tttgatataa taaaattgtt taagtattaa ttatgagtat taattaatgg tatagataat
     6181 tttaatataa ctgtatttat aaataattat aaaataataa attaattttt ttatactgac
     6241 ttttttatag tataaatcgt tatatataat gacggaggtg ataatagtga atgatgctcg
     6301 gattatatca caaataaaag caggaaaaaa agaattgttt gatactctaa taaaaaaata
     6361 ctatgatagc atctactgtt attgttatcg ccatgtagac aataaaatga ctgcacaaga
     6421 tttaactcaa gatgtatttg taacagttat aaataatata gaaaattata aacattatgg
     6481 taagtttgaa aactatttgt atgttattgc aaaaaacaag tgtaaggatt tttggaagaa
     6541 aaaacaacca atatatttag agtcattaga taaggagata tcaaatcaag aattatctga
     6601 tatagaaaat aaaatgttta taaaagaagc tttgactaaa ttaccagaat tagaatttga
     6661 agtgataatt ttacggtatt accatgattt aagatttaag gatattgcta aaattatgga
     6721 gtcaagcgtc tctgtcacaa agtacaggat gagtagggca ttaaaaaaac tagaattata
     6781 tctggaagga ggtaaattat gaataataaa gaaataaaaa gaagtctaca gaagtataag
     6841 gttcccaaat atgattcttc agctcttgaa aagactatta aaactataca tgagttaacg
     6901 cttagtaata cttataaaag aatgaataat aaagactttt ttatatctca aattggtttt
     6961 ataagaaaaa gaacttggat tatgcaattt ctaatactat gtacaatatg tatattttta
     7021 tttagtacat cagcagatag taattcattt tataatagtt tatccttgat ttctatagca
     7081 tcaccacttt ttgtactgac aaatatagaa gaattaacaa aagtgtataa tagtagtatt
     7141 ttagagatag aattttcaac aaaaaataca ttacataaaa ttattgctac tagaatgctt
     7201 atatttggaa taagtgattt attgtttatg attattattt tggtatctgc aaaacaaatc
     7261 attgatgtaa acattctgta tataataatt tatatactag ttccttttaa cctaatgtgt
     7321 ataggatgta tggaaattat aaatagaaat aaaggtaagc atacaaatta ttattgtatg
     7381 atatacggta tatctcttgt attattaata gttacactga gtaactttag agataatcta
     7441 tatcaatttg aagcaataaa tcaatggcta attgtttttc tagtcacagc agtaacatta
     7501 atatatgaaa taagaaaaac gtggagacat ctttgttcat ttgaaaatat tattatctaa
     7561 gtaaaaaata agaatacaca gtatcaggag gaatagtatg gagttaagtg tagaacgttt
     7621 aacaaaacag tataacaata aaattatagt agataaaatt gactttaaat taaatgaggg
     7681 tatatatgga tttttaggag aaaatggagc tggaaaaaca actcttatga gaatgatttg
     7741 cggaatatta agtcctacat ctggagaagt aaaggtggat ggtaaaaata atttggatat
     7801 gggagaagat tttagagata tgattggata ccttcctcaa aactttggat actacccaga
     7861 ttttactgcc aaagaattta tgctatatat agcatcatta aaaggattaa tgcctagcta
     7921 tgcaaagaga aaaacacaag aattattaga aatggtagga ttatcagagg taattaataa
     7981 aaaaataaga acattttctg gaggtatgaa gcaaaggcta ggatttgctc aaacactact
     8041 taatgacccc aaaattttaa tattagatga gccaacagca gggctagacc ctaaagaacg
     8101 agttagattt cgcaacctaa tttcagaatt ttctaaggaa aagattgtta tattgtctac
     8161 tcatattgtg tctgatgtag aatatattgc agataaaatt ttaataatga aaaaaggaag
     8221 atttgtttta actggctcaa tagaagaagt aacttcaaag ataaaagata aggtatggaa
     8281 atgtgtggtc aatgagaatg aaatagatta tatgtctgat agatattgtt tagcaaatat
     8341 ccatcattct ggtggagttg caaatcttag aattgtaagt gatataaagc cacatgacaa
     8401 tgcatgcaat ttaaaaccaa cattagaaga tttatattta tattgtttca acgaagtact
     8461 ataaatatac ataaggagat aggtatgtct acattaatta aatttgagtt aaaaaaaata
     8521 ataaaaagaa agtctacaat agcagttatt ttaggaagtt tattcttaag catcattctt
     8581 tttgcattaa tgccaattca ggaaaaagct aatgatgaaa gtggaaaaga gtataggggt
     8641 ttacaggcta ttgagctaaa aaagcaacaa tctaaattaa atgagggata cataacagat
     8701 aaaaaagtaa aaaactcaat cagagattac caaaaaatgt ttaataatcc aaataatatt
     8761 acaacagatg aaggtgggtt ttcaaatttt aaaccagata tttattggaa attcttctat
     8821 ccaaataaag ctttttataa ttttataata gagtcatata gtaatccaaa tgattataat
     8881 ccagaaataa ttaaatcaat gcctttagaa gatggagtta atttttataa atttagaaat
     8941 gaaaaagtaa atacaatatt aaatatggaa tatcctgata aaaattacac acagaaagag
     9001 aaagattttt ggttgtctaa aaataaggat atagaagagc cttatttaaa tggatattat
     9061 caaggatgga attattttct attggctaga gatttactaa tttttactat agtagctgtc
     9121 tgcataagta cagcttctgt attttcagga gaataccaat ccggtgctga ttcaataata
     9181 ttatcttcta aatatggaaa aacaaaagtt ataacagcta aaatagtaag ttccttttta
     9241 tttggtacga ttatttttac aataaataca attgtgtcta ttggtatact tcttttatct
     9301 tttggaattg atggatggaa tttaccatta caacttacaa ccagttcaat tccataccca
     9361 tatacatttt tacaggcaat attactaagt gtgttaatta tttatatgat attaatacta
     9421 ttaattagtt ttacactttt attatcatct tatatgaaat caccttttcc agttttaatt
     9481 acaaatataa tattactatt tgtaccaata tttttaaaat atagtgaaac aaataagttg
     9541 tataatcaaa ttctcaatgt tttaccatca aatgctattt cattttcatt tactaattat
     9601 gtagattata aatttggtaa tttaataata gctttaccta caatgattat ttttacatgc
     9661 ttagttctta cagttgtttt tttacctttt attaaaaata attttaaaaa acatcaagtt
     9721 aactaaaata tatattataa aaagaaggtg tcttaaaatg aactttttag cttattaggc
     9781 actcttcttt gtattaattt aaatatattt tagtattaga ttaaatatgt tttcatcatt
     9841 ttaacaatga aaaagagctt tatctctatt ttgagatagc tcttataaag ttaaatatta
     9901 aattgataac tataaaatat gtagtttgaa tttataattt gtgttataat catactgatt
     9961 tttgtacatc agttcttaca ttaacattgc ctgcttttag attttctcca atagtaagac
    10021 ctttttcttt tagaacatat tcaaaagtca ttagtggaac tccacctttt ctaccaccta
    10081 atatttcggt tccttttaaa tcattgtaag agaaattagg catttttttc ctagcaacta
    10141 gaaaactacc atctcttttt gtcaattgag caaagttcac tgcataatcg tttttgtttt
    10201 gattatatac atatatagat gcttcaggac ccattaaacc tacgtttgct tcacctgaaa
    10261 tcaaagctgc catagtttta tctgcacctt gggtatttat aaggtcaatc tttatacctt
    10321 catcttcaaa gaatcctttc gtaatagcag catactgtgg agcataaaaa acagagtgag
    10381 taacttctgc tatagttact ttatccagat ttttagacga agaagattca ccttttttgt
    10441 cattagagtt aaaatcacat ccagttagac atattgtact tacaatgata gagcttgcta
    10501 ttgcgactat ttttttaaac ataataaata cctcccataa gacatacgaa tagtatatag
    10561 tattaaaaat ttattgtagt tgttacaaaa atgtatctat gcttataata tttctacaaa
    10621 aacttaataa taattttcag ctttatgttt agttttttca gtaatgtgta atattgg
//
LOCUS       DAMJZE010000137        10631 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJZE010000137 DAMJZE010000000
VERSION     DAMJZE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.140"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(173..742)
                     /locus_tag="PC541_002868"
     CDS             complement(173..742)
                     /locus_tag="PC541_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2840314.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            945..1172
                     /locus_tag="PC541_002869"
     CDS             945..1172
                     /locus_tag="PC541_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2840315.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1391..2134)
                     /locus_tag="PC541_002870"
     CDS             complement(1391..2134)
                     /locus_tag="PC541_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2840316.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2302..3174
                     /locus_tag="PC541_002871"
     CDS             2302..3174
                     /locus_tag="PC541_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2840317.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3174..3839
                     /locus_tag="PC541_002872"
     CDS             3174..3839
                     /locus_tag="PC541_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2840318.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3860..4717
                     /locus_tag="PC541_002873"
     CDS             3860..4717
                     /locus_tag="PC541_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2840319.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4873..7419)
                     /locus_tag="PC541_002874"
     CDS             complement(4873..7419)
                     /locus_tag="PC541_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2840320.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVISKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7419..8105)
                     /locus_tag="PC541_002875"
     CDS             complement(7419..8105)
                     /locus_tag="PC541_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840321.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8188..9177)
                     /locus_tag="PC541_002876"
     CDS             complement(8188..9177)
                     /locus_tag="PC541_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2840322.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9164..9850)
                     /locus_tag="PC541_002877"
     CDS             complement(9164..9850)
                     /locus_tag="PC541_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840323.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10350..>10631)
                     /locus_tag="PC541_002878"
     CDS             complement(10350..>10631)
                     /locus_tag="PC541_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840324.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3749 a         1758 c         1546 g         3578 t
ORIGIN      
        1 atattaccta acatctacaa taaaatatac tcttaaaata aaattatata attaaaaata
       61 ctctataaaa gctaaaaagt ttttctattc gctacatcta aaaataatta gatagaggta
      121 taaatgtatt tagtttattt atacctctag tcttttataa attatatctc tactaaattg
      181 attttttagc acgaataagt gtgctcttgg agataccagt catcttcaca acctctctat
      241 atggcttaga atttaataac cccaatgcat attgtatctg ttgattgctg tatttctttg
      301 gcctaccatc tttaaagtcc tttttctttt tagcaaattc ttttcctaat tgagtacgtt
      361 ctattatcat atttctctca agttctgcaa ctgcaagtaa tgtagtcaaa aagaatttac
      421 ccattgaagt attttctaaa aggcctatat ttaaaatatg aacacttact tctttagcaa
      481 ataaaccttc tattagttgt attccttctg atgtacttct agctagtcta tccagcttag
      541 ttgcaacaag taagtctccc ttttttagag atgatataat tttttcaaat ttaggcctgt
      601 ttgtagtagt gcctgtataa gtttccttta ttattttagc atcattatat ttattcaaaa
      661 tttcttgctg ttgctgatac aaactgttgt tgtctgtttg attttttgta cttacacggc
      721 aatatccata tataataatc atcacttcct aacataaaga ttttgtacag aagttaccca
      781 gaagcttgtt tttacttatc tttatccatt ttactaaaac gatttttggg tgtcaatact
      841 gttaactttt gacaccataa taattccttt ataatttgtt tgactatgtc aaaaattacc
      901 aatataatga aattaatgag tttatacaaa ttggaggaaa taatatgaat agtctgttaa
      961 aagaagcaat agatgaatta aaaaacatcc attgcgggga agttttttta ttaaaagaat
     1021 tgttcaaagg ctatgaatgg aacaggttgg agatgaatat cagattgaaa ttaggaaaac
     1081 tttttttgta tgaagtggta actaatccca atttaaatgt agaggtcatc aataaaaatt
     1141 ctgcttatca gcaaatgtat aaaaaaaaat agtgacttaa atgtatataa tagtataatc
     1201 ttcttattga ttcaaaagtt aaaactttta atacaaaaca atcattctat tttaacattt
     1261 atcaatatgt ctaaatttat ataattaaca catacgaata gtattgttta aatcacatta
     1321 tttaagatag ataaatctta accctactcc agcataaata ctaaaatagg gcttagaatt
     1381 atctatattt ttatcccata aaatgcttag ctcttcctct tgattgaaca tatatatctg
     1441 gggaaataca atagaagcgt aaatttataa aatttccaaa gtcagaaaac ttcagttcaa
     1501 acttaggtat accaaaactt tcaggtgcta tttcgtcaac gccaaaatag atgataattc
     1561 cattatctgt aagataaaat gcttggtctt caggtataac taactcaaca ttagaataat
     1621 acatttctct attttcattt atttgagatt taatgtaatc agtaattata ttaatataat
     1681 caactccatc tctaaaaatg tcattaagta aaagttcttt tccagttgtc aaatctatat
     1741 tgtaattatt caatgtattg tatttaggcc catagttatt agcaaacccc ataaggttta
     1801 atatcatact cagtacataa tttttattaa aagtaactgc gtaactggta gatacccaat
     1861 agctttgttt tggaagtgaa ttttgagaag ctgcgttatt atattctcgt tcttcttcct
     1921 gtaagccatt tttaaaatta tttacagtca ttcttacagc attgtttatg ttatttaaaa
     1981 taactgggtt tacatacgtg gattgatttc taatattgta tataacagga tactttacat
     2041 catatgaaaa atacttactt ctttcaaaat cttcattaaa ttgtacatat aatatattat
     2101 atgtacaggt atttgtagat tgataatttt ccattaaaat cctccccctt ttataaaaaa
     2161 atcagataca atataatatg cttaaaacag taataatgtt aatggtaaat gtaaagaata
     2221 gaaagtatct aaaacagcaa aaaatgacta attttgtggt ataatacata ggattgagag
     2281 ttattttata ggaggaaaaa tatgttatta ttagcagata aatggaaaga ttatgaactt
     2341 atagatatgg gaaatggaga aaaattagaa cgttggggaa gttatgtact tagaagacca
     2401 gacccacagg ttgtatggcc aatggaatct gaatgggctt tatggaaaaa ccctcatgga
     2461 cattatcaca gaagcaataa aggtggaggt caatgggaac acaaaaaaag atatccagaa
     2521 caatggacta taaattataa aaacttaaag tttcacatta aaccaactgg ctttaaacat
     2581 actggattat tcccagagca agcagctaac tgggattgga tgatagagaa aataaagaaa
     2641 gctaacagac ctataaaagt gttgaatctt tttgcatata caggtggagc tactgtagca
     2701 tgtgcatctg ctggtgcaga agtatgtcat gttgatgctg caaaaggtat ggttaattgg
     2761 gcaaaagaaa atatagaatt atcaggtctt aaaaatcaaa cagtaagatt tatagtggat
     2821 gatgttgtaa agtttgttga aagagaaata agaagaggta aaaaatatga tgcaataata
     2881 atggaccctc cttcttatgg aagaggtcct aaaggagaag tatggcaaat agaagaaaag
     2941 ttatacagtt ttgtagattt atgtatggga gttttatcag ataacccttt atttttctta
     3001 attaattctt atacaacagg attttctcca atagttcttg aaaatgtatt gtctacaaca
     3061 ataggtaaaa agacaaatac aggagatata tacggtgggg aattaggaat accagcttcc
     3121 agagatggta agatacttcc ttgtggtata tttggaagat gggagaaaaa ataatgataa
     3181 aagtcatata tgaagataac catttattag ttgtagaaaa accagttaac atattgtctc
     3241 aaggagataa tactaatgat aaagatatgg taaatctttt aaaagactat attaaaaaaa
     3301 agtataacaa acctggaaat gtatatattg gtttaataca tagattggat agaccagtag
     3361 gtggagttat ggtgtttgca aaaacatcaa aagcagcatc tagattgtct gagcaggtaa
     3421 gaagtaagac tttcaaaaaa acttatctag ctgttgttca tggcaagata aaatctaact
     3481 cagatatact aaaagatttt ttatataaaa ataaaaaaac taatatggta actgtagtga
     3541 aaaaaagcca taaagatgcc aaagaagctg aacttaacta taatgtatta gattttaaag
     3601 aaaatctaag tttagttcaa gttaatttaa agactggtcg ttctcatcaa ataagagttc
     3661 aatttgcaag cagaaaacat ccattatacg gagaccaaag atatggcaaa gaaataaata
     3721 agattggaca acaaatatct ctttggtcca ataagataga aatttgccat cctactacaa
     3781 aagaaaaaat ggaatttaca tgtcatccac ctgacaaata tccatggaat atattttaaa
     3841 tctcaaatga ggtgattata tgagtaaaaa attttcaata ggagaaatgt ctaaacttca
     3901 taaaataagc atacagacac taaggtacta tgaccaaata gatttattta aacctgttca
     3961 tgttgaccaa gaaagcaatt acagatatta ctcaattgac caattttcac aattagatat
     4021 tataaaatat ttaaaatatt taggtatgcc tttaaaagaa attaagtcaa agcttgatga
     4081 aaataatgaa gaaattctaa aattactaac aaataaaatt aatatagtgg atgaaaaaat
     4141 taaagaatta gagcttataa agaaggtact aagcacaaag aaagaaacta tggaaaaagg
     4201 tatgaaagta gatgaagttg gtaaaatagt gagaaaacat attccacgaa gaagtatctt
     4261 atctatagag tatgaagaag gattaagtag tgaagaaact atggaacttt ccagaagaca
     4321 aatagcaaat atattagaag ataatatatc tgtgttctat ggtggagtaa gtggacttgt
     4381 atctatagat aaatttttaa aagaagataa agttatttat gacaatagtt ttgttactgt
     4441 tgaaagagac ttatttgata taaaatcaca aaaacatata acagaaattt catctggtga
     4501 ttttatttgt atgacatata aagggcctta caaagaaaat tataccgctt ttaagaaact
     4561 ggttgattat gttgaatata aaagaatacc tattgaaaat tatatatatg aaatacctat
     4621 aatagaccct ctgtctacaa acaacgaaga ggaacttcta acagaaattc aaatacaagt
     4681 taaaaagaga gaaattgact taagatgtct agtgtagtaa tctagtttac ttacacatct
     4741 taatatagtt gtctattatc aacatatgat ttttctagat agtgattcct aacagtgaga
     4801 ttttcaaaaa ataggaggaa atatggctaa tgctaattcc ctcctatttt taattaagcc
     4861 atatcatttt aattaagcca tgtcatcacg aagtgcatca ataatatttt ctttttttat
     4921 cttattaata gtatacaaca ttgtaataaa tactataaat aaaacactaa atatactaat
     4981 tccaatacta aaccatggaa tcacaaaatc aatatcatct gtaaccatta ctttatgaat
     5041 tagccatgaa gaaattactg ctataggaag tccaaagaac aatgctctca tgccataaaa
     5101 ggcacactca aaattcatca tcttttggaa atcatgctca gacattccta cagagcgaag
     5161 catggcaagt tctctacgac gtaacttaat attagttgaa attgtattaa acacattggc
     5221 aatcgaaatc agagaaatca taattatgaa agtataagca aaaacattgg caataaaaat
     5281 gtaattacgg ttctcatcca tcattccata catattgtac agattgtact cattcgtaac
     5341 tccctcactc tcaataattt gctccatttc tgcaactgac tgagtaggat ttttcgattg
     5401 aaagcttaaa ctttcaattc ctgtagaaac accaggaatc tcaaacttat ctttggctga
     5461 ataaggagcc aatactctga agcttgcttt cttcacatct gaacttctga ttcctggagg
     5521 agaatcaggt ggcactactt tgacaaatga aatattcaca ttttgtctct caggtatctc
     5581 caacttgcca ttagtttcag gagtgaggct aacattcata gaagaactgt tgaaaatatc
     5641 aataatatta ttatcctttg ttaccataga aacagcaatc agttttgcat tttgtccagt
     5701 atattcttct gctggtaatc caagactttt aataatattc agataagttc tatcatcaag
     5761 aaattgcata tctactggta attcaagcat ttcactcata gaatgtgaac cctcatagtt
     5821 caaataatcg tctgtaagct cacttacttt ggcaacacat gaataggtca taagtttctg
     5881 atacaaacct tcgtaaacac catttgcagt ttttagctta tcgtaaagct tcaacatttc
     5941 gttgtctttc ataccttgtg tagaaaaaat aatatcctgg tcagtaacct ctgacctctg
     6001 atttgacatc tgttttaggt ctgctactaa agcacttgta gatataaata gaactatgct
     6061 taaaacaagc gacaatacaa tacttcgata acgtttcttg tttcttttaa agtttttcat
     6121 agcaagaact ccctcaagac cataaactcg ctgtgttagt tttgatgtct tcacagattt
     6181 agattcaatt tttacctcat ttgtctgacg aatacactcc atcactggtg tgttggcagc
     6241 ctttcttgct ggtatgtaag cagaaattaa gattgtaacc atacttacca ctgctgcaac
     6301 gatgattgca gggatagaca atgttaatgt caacggaacg tttacataga gaatatttgc
     6361 aaagttttta gatatgacag aaatcacaag tccaatactg actataccga ttgcaacacc
     6421 aattggtata ccaatcgcac caatgcaaat tccttcaaat agcaccgaat ttcgtaactg
     6481 ttttgctgta gctccaacag atgaaagtat tccaaattga tgtgttcgct cgttcaatga
     6541 catgttaaat gaattatata ttagaaaaat tgagcctagc atgattatgg caagcacaat
     6601 gccaccaacc gagtacaaaa acatattgaa caaattgtta tcagagacac ctatgaatcg
     6661 caacacattg tcattgaaaa tggaagcatg gtctttagtg ctacttgtgt aagagcgaat
     6721 ctgatgtgga tttttcaatg tgacaaaaag gctaagactg tcagtcttga cttgtttatc
     6781 ttctttggtt atcaaggtat atcctggtgc agaagattct tcaaatacgg gctttctaca
     6841 gatacccaca actgtataag ttttcttatc ctgtggcaca aaaacttcac tcccagcagt
     6901 gtatgggtca ctctgaccaa gcttttcatt ttcactcatt cgtttcccta catccaaaga
     6961 aatagtatcc cctactgcaa atttgacacc accatctgtc gcaactttac ttgaaacaag
     7021 aatctctcta ctattttcag gtaatcttcc agaaattaaa gttataggca aagtatcaaa
     7081 agcttctttg ctaaatccag caataaaaag atatggttta tctggatttc tgttaccttc
     7141 aagtgttgca tagccaatat tttcaaatgt tgcagtattt gcaacttcat cattgactct
     7201 ttgttcctgt acaaaagaag aatccacatc caaaaactgg acatgccaat caccatattt
     7261 tataatagct ccatttatca tatagttcat caaagaaact ccaaaggtag taactgctgt
     7321 aatcatagca gctgacagaa caactcctat aattgttaca agagttcgag ttcgattctt
     7381 tttcaatcct tgcagagcaa ctttgttaaa aatgttcatt aacgattcac cttcctctca
     7441 tcacgcacta cttttccatc tgaaatacag ataattctat ctgcctgtaa agcaatgttt
     7501 tcatcatgtg tgacaataat gagagtttgg tgatatttac tatggctctc tttcagtaac
     7561 tggataatct cttgtccatt tttactgtct aagctacctg taggctcatc agcaagcata
     7621 actgctggtg cattcatcaa tgcacgacca atggcaacac gttgctgttg gccaccagaa
     7681 agttgatttg gtaaatgtgt ttttcgttct tttaaaccaa gtaattctaa caagtcattt
     7741 aaccgttctt gattgacctt acgcttatcc ataagtatag gcaatgtgat attttccact
     7801 acatttaatg tgggtatcag attgtgaaac tgataaatca atccaacttg tcgcctacga
     7861 aaaatagcaa gtttttcatt gctttggcca tatacatctt gcccctccag atatatcttt
     7921 ccacttgttg gtacgtctac tcctgcaatg gaatgtagta gtgtagattt accagaacca
     7981 gaagaaccaa tgattgcagt aaactcccct ttttcaatag taagtgagac atggtcaagt
     8041 gcagtaacct tattttcatt cttaccataa accttacata aatcttcaat ctttaaaaac
     8101 gccattatat aagtctcctt tcatatttta taaatatatc atagaacttt caacttacat
     8161 ttaagtgaca tttaaatgag agattcgtca ctttggaaaa cgaataacaa atattgcacc
     8221 accttgtggg tgattttgtg cagtaattgt tcctccttgt ctcataataa tcgtcttaca
     8281 aagtgccaat cctattccat aaccggctgt gcttgaattt ttcccacgat aaaatctgtt
     8341 aaatagacat ggtaaatctt cttttttaaa gcctgctccg ttgtcatgaa tagtaagttg
     8401 agtaaacaaa aagttgtcct cacaaatgat atcaattttt ccattatcac ccacactctc
     8461 catgcagttt ttaaatatat tttgaattgc ttctgaaagc caatttaaat cacccaaaat
     8521 ccttactcct tttggtatat caatatgtaa agtgatattg tgcagttcca tgggaatcaa
     8581 aagttgatga agtgcagaac taatcaaatt attcacatca atttgttctt tttgaaagac
     8641 tacaatacct gcatctaaac gtgataattt taaaagggaa gtaatcagcc agtccattcg
     8701 tataagtaat tcctctgttt ctcgtatcaa ctcttttctc tcatcttcat cagaagtatt
     8761 ctctaacagt gacaaaataa gagttactga tgtaagagga gtgcgaagct ggtgggctat
     8821 gtcagctagt gagtcagcaa ggtgttcttt ttcttttttc agtgcgtaat tttgctctct
     8881 aatgcgtaac gtcattttag ttatttcact ttgtagaata gaaagctcac cctcatctga
     8941 ttcagcaata tacagatggt tagcattatg aagcacaaga ttaatttgtt ctgaaatctg
     9001 tgcaatactt ttgtatcgat ttttagtaaa cacaaaaaat gctattccaa aggtagtggc
     9061 agaaacaata gaaagtattc cagtcataat actgattgca aatcccaggg ctacagtgag
     9121 aatagtaatt aataagaata aaatagcaaa ttttcgaaac tctctatttc gaaacataac
     9181 ttccccccaa tctataccct gtcccacgaa cagtcaaaat tatttgtgga cttgaagggt
     9241 catcttctat cttctctcgc aagcgtttga tgtacacagt caatgtgttg tcattaacaa
     9301 actcacctgc cgcatcccac aattcatcaa gtaatcttac cctcgtaata atacttttag
     9361 gattgctaat aaacaccaat aacaaacggt attctaaagc tgaaagaaaa acctcattgc
     9421 cattcttttt cacaacacca gttgctgtat ctacataaag cccacaaatt tcaaaagttg
     9481 acccaaaacg cccacttttt cgtagtgctg ttctaattcg cgcaatcagt tcacgtggac
     9541 gaaaaggttt agtaatataa tcatctgcac ccatattcag tccagtaaca acacttgatt
     9601 catcaccgga agctgtcaaa aagataacag gaatgtcttg cttttctttg atttctgtgc
     9661 aaatcgtaaa cccatttcca tcaggtaaag aaatatcaat aagtgccaaa tcaaatttat
     9721 tcttagcaat ctctacaagt gcttcacttc ttgtggatac atgggttact ataaatcctt
     9781 ctgaattaag caaaagaata agatttttgg caattgcttt atcatcctca actaaaaata
     9841 tccgtttcat ttactttcat cctcctttat tttattagtt ttatatattc cagcagagca
     9901 atagtacact actattttac ttatcattac acaattttca gtagtttctt ttgttcttat
     9961 ataatccatg cataatacct ccattgattt attatatttc ttataacata aatacacaag
    10021 ataagacaaa aaacataatg taagtgcaag ttttttaact cactaactta taaacataca
    10081 ctcctactaa cactactttc ttgatgacac atcttacaag cttatattca tacatcaatg
    10141 aaaaattaaa tttatccatt tattaaaata aaaggcaata gcttctatag ctaatgccct
    10201 ttatcttaaa atgatttaaa tactatacaa tttttataaa tggctataaa taaactcgta
    10261 tagaatataa aatttatcta cagtcttttg ttatacaaaa tttataacct cttatagaac
    10321 aaatttacca ctttgcaaac ttatcacttt tactcagcta tattataaac ttttaattta
    10381 tctttgttta aatgtttctt cttaacttct actaaagctt ttagcgtatc caaagatgtc
    10441 attatttcag tagaaaattc aatggctgtt cttctcatct taaatccatc tcttgtagag
    10501 tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct tatagcatct
    10561 aatatgtttg gtctaggttc ttcaattcta tttactatat cagcctctat tccattttct
    10621 cttaaactct t
//
LOCUS       DAMJZE010000138        10607 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJZE010000138 DAMJZE010000000
VERSION     DAMJZE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.141"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            72..806
                     /locus_tag="PC541_002879"
     CDS             72..806
                     /locus_tag="PC541_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840325.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1017..2198
                     /locus_tag="PC541_002880"
     CDS             1017..2198
                     /locus_tag="PC541_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2840326.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            2182..2754
                     /gene="mobB"
                     /locus_tag="PC541_002881"
     CDS             2182..2754
                     /gene="mobB"
                     /locus_tag="PC541_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2840327.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            2873..3148
                     /locus_tag="PC541_002882"
     CDS             2873..3148
                     /locus_tag="PC541_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2840328.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3391..5091
                     /locus_tag="PC541_002883"
     CDS             3391..5091
                     /locus_tag="PC541_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840329.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5454..6641
                     /locus_tag="PC541_002884"
     CDS             5454..6641
                     /locus_tag="PC541_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840330.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6701..7864
                     /locus_tag="PC541_002885"
     CDS             6701..7864
                     /locus_tag="PC541_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2840331.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8015..8818
                     /gene="zupT"
                     /locus_tag="PC541_002886"
     CDS             8015..8818
                     /gene="zupT"
                     /locus_tag="PC541_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2840332.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            8913..9473
                     /locus_tag="PC541_002887"
     CDS             8913..9473
                     /locus_tag="PC541_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2840333.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            9723..10436
                     /gene="rgbR"
                     /locus_tag="PC541_002888"
     CDS             9723..10436
                     /gene="rgbR"
                     /locus_tag="PC541_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2840334.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         4195 a         1023 c         1911 g         3478 t
ORIGIN      
        1 ttgaaaaaag agataactgt aaattgagat atagataaat actataaaat ttaagaaagg
       61 aggattgatt tatgaagtta aaaaggtcat tagtttgtgt aactatttta ggtattatac
      121 ttgttggatg tcataaagaa aatactaaag agaaaaatca agtagcaagt aaagcaacac
      181 agcaaaaaac tatgacaaaa gttcaaaatg atgttaatga aattatgaat aaagattata
      241 agtacattat aaagaatatg ggtatacctt ataatacatt ttattatata aaacctaaag
      301 ttttaaaaga atcaaataca atgcaagata taaatacatc aagttatatg acattagttt
      361 atccaaaata tactggaaat gatgaactag atggaagtgc tttatatgtt gatattaacg
      421 aaaataaagt tgtcaatgtt gaaaccaatt ctttctcatc acaaggtata tcagtcattg
      481 atgctgaatc tagtatagtg attgaaaaaa gtgaccatga aaaatcggct gtatcactag
      541 aaaattttag acatattgac ctgggagagt atgttggtgt tgaagattcc agaataaatg
      601 aaattgtagg agatgcgaat tatgatttaa cagcatataa tcatgaaggc agtaaagtag
      661 ttaagagtta tagattgaaa gaagataaca aaatattaaa aaaagaggta cttacaataa
      721 gtattgtgga taacaaaata aaatctataa aaactattga aagtgataaa attgtaaaaa
      781 taataaaagg aactttatta gaataaaata ttctaaagca tatgatgctg tttcaagata
      841 gaaggaaatt tctagctggg aacagttttt ttattagatt tataaaattt gaactgcact
      901 tatagataac tattagttat aattaaatat aagataaaag tatttccaac ttttataatc
      961 tataatatat tacatagaaa tatcttaaat aaaaaatata ttggggagta tttaaaatga
     1021 tagaattaga agatgccata aaaattattg aaaaaaatgt tttaccaata aacagggtca
     1081 agatagtaaa tcttatagat gcagtaaata aggtagttgc agaagatata tattcaacaa
     1141 ttgatagtcc tccatttgat aggtcaccct atgatggtta tgcatataat gcaaattctg
     1201 aaaatagaaa tttaaaggtt atagggactt tgtatgcagg agatgtattt aatagggtat
     1261 taaataaaaa tgaagcagtg aagataatga ctggtggaaa gataccacag ggtgcaaatt
     1321 gtgtaataaa aaaagaagat gtaacagtta taagtgatat gataacttta aatataaaat
     1381 taaaggaata cgataattac atatttaagg gtgaggatat aaaaaagggg caattgatta
     1441 ttaataaaaa tcagaatata ggatatgatg gtattggagt attagcaagt cttggaatat
     1501 ccaaagtaac tgtttatgat gatttaaaag ttggaatttt gaatacaggt acagaactac
     1561 aagatataaa tgagacttta gagaatggta aaatatacga tagtaataga tataccttat
     1621 attcaagatt attaaaactt ggaatacaag cagatacaat atgcaattgt tctgatgata
     1681 tattggagtt agaaaaaaag gtaaagaata tgttacagag tgtagatttt ttaattacca
     1741 caggtggtgt ttctgttgga gataaagatt taattccaga tgtattcaaa aaaatagggg
     1801 caacagaatt attttggaag ataaacatta aacctggtgg agcttgttat gtaggtacat
     1861 tgggagaaaa gctattattt ggtctttctg gtaatccaca tgcagcaata gtggtatttg
     1921 ataatgtgat agttcctgtt attgagtatt taacatataa gaagggaaca catcatataa
     1981 aagctatatt taaaggagaa tttaataaac tttcaaataa agatagattt gtaagtggaa
     2041 aactatatac aagtgaaggt aaattgtatg tagagctaaa tgataagaaa gatgcaaaag
     2101 ctagattatc tgcaactcta agaagtaatt gtatgattaa gattaaaaag aatgagacaa
     2161 taaaagaaaa tcaattagtg gatgttgtta ttagatagtt ttatgaaagg acacaatttt
     2221 atgtataata tattgtcagt tgtctcatat tcaggagtag gtaaaactac attgattgaa
     2281 ggcataataa aagaattaaa taaaaaaggg tatcgtgttg gagttattaa acatacctgt
     2341 catgattttg atatggatga ggaaggaaaa gatacttata aacatagaaa atctggggcg
     2401 agaaaagtct gtataatatc agataaacga gtagcatata ttgaagaact aaaagagaag
     2461 aatccattat ataaaatgat tcaattatat gaagatatgg acttgattat catagaagga
     2521 tataaaaatt atagatttaa aagattggaa gtaactagaa aaggtaagta tgaggatatt
     2581 ttatctaata agtctgattt gataggtatt gtcagtgata tagaatataa attgaatatt
     2641 gaacagttta atttaaatga ttataaaaat atatcaaagt acatagagtc ttgtataaaa
     2701 aatgaaattt taagtataag caatgttgaa ataaaaaaca tattaaaaga ataattttaa
     2761 gatgttcttc ttatataaaa tgatataatt atttatataa ataaagatat aagaaattta
     2821 tcaattttat tttgagttat aaaatattaa agaaattgta ggaggacaat atatgaaaaa
     2881 aatattagta gcctgtggag caggaatcgc aacatcaaca atagtttgtg acagagtaga
     2941 aagattggtt aaagaaaata atatacaagc agaagttgag caatgtaaaa ttgctgaatg
     3001 ttcaacaaaa caagaaggtg ctgatttaat cgtatctact actatactac caacaactta
     3061 tgatatacct acaataaagg caacaggata tataacaggt ataaatacaa cagcattaga
     3121 taaaaagata ttaaatgcat tgaagtagtt ttgtatttat aaaaaaaatt tagtattaaa
     3181 tatagagttg tatctttata aaattttaaa tatagagata tagctctttt tatgttgttt
     3241 caagtataca actatatgat ataaatcttc aattttatga aaaagattgt tattactttg
     3301 taagtttata aaagatttaa agttattgtt atgttatact aatttatggg tattttttag
     3361 aagtactaat aaaggaggga tttaatactt atgataaaac aatttgtcaa atattataag
     3421 ccatataaga aaatttttac actagacctt atagcagctt ttctattttc cttatgtgat
     3481 ttagtttatc ctatgataac tagaaatata atggatgacg ttgttccaaa taaaaactta
     3541 agaatgttag tagtttttgc agtagcactt atacttatat ttatagcaaa agcagggctt
     3601 aactatttta tgcagtattg gggacatgtt attggtgtag atatgcaagc tgatatgaga
     3661 aatgaggtat ttacacactt acaaagactt ccaaatacgt attttgataa taacaaatct
     3721 ggagtaacta tgtctagaat agtaaatgat ttaatggata taacagaact tgcacatcat
     3781 gggcctgaag atttattcat ttctatagta atgttggttg gttcattctt tatacttata
     3841 gatataaata ttcctcttac gcttataata tttgctatat taccgtttat aatttggttt
     3901 gcaatagcta aaaaagataa gatgaatatt gcttttatga agagtagagt tactataggt
     3961 gatgtaaatg ctactttaga aaatagtata gcaggtatga aggtaactaa gtctttttgt
     4021 actgagaaag aagagttaaa taagtttgta agaagcaata aattatttag aagagcaaga
     4081 caagattctt ataaggtaat ggcagaatat tactctggta tgaatttata catggatatc
     4141 ttagagtggg tagttgtaat agctggtggt tattttactt atataggaaa gattactttg
     4201 ggtgattttg ctgcttatat actatatgta aaaatgttta tacaacctat gaaaaaatta
     4261 ataaacttta cagagcaata tcaaaatggt atgacaggtt ttaaaagatt catagagata
     4321 atggaacaag accatcaaaa agaagctaaa aatcctattg aacttgaaaa tgtaaaaggt
     4381 gacattgaga tagaaaatat atctttcaca tatgaagata agactcaagt tttggataat
     4441 ttaagtttat ctataaaagc tggtaagact atagcattag ttggaccttc tggaggagga
     4501 aagactacat tatgtaatct attgccaaga ttttatgaat ttgataaggg tgatataaaa
     4561 attgatggaa agagtataaa agatgttagt ttaaaatcat tgagaaaaaa tataggtata
     4621 gttcaacaag atgtattttt atttacagga actataagag ataatattct ttgtgggaac
     4681 cctaatgcta cagatgaaga aatgatagct gctgctaaaa aagctagaat acatgacttt
     4741 gtagagactt tgccagatgg atatgatact tatataggag aaagaggcgt aaaattatca
     4801 ggtgggcaaa aacaaagaat ttcaatttca agaatctttt taaagaatcc tccaataata
     4861 atattggatg aggctacatc tgctttagac aatgtaacag aaagagaaat tcaagagtct
     4921 ttagaggaat taagtaaaga tagaacaaat ttagttgttg cacatagatt gactactata
     4981 aaaaatgctg atgaaatcat agtattgacg gataaaggaa ttgaagaaag aggtactcat
     5041 gaagagctag taaacaaaaa tggagtatat agtaggttac acaataacta gtatacaaat
     5101 tttacaccat agctaacata cacattaaac ttacaaacat aggatatatt aagattcata
     5161 aagacaattc attttatatt tataagatgt tttatgaata aaatgtatcc tatgttttat
     5221 tttttgcaaa ttacatttaa ttctctttgt aagaatagag ttaaatagcc aatggtcggg
     5281 ataacgtttt tacaaaaaga ataatttaga aaactaactt taatttatgg aatttatcga
     5341 attttatgaa aaaaatcgaa ttttgacttg aaatgttttt caaaagtagt aaaataatgg
     5401 ataactatac atacatttta taaaaatata tttattatga ggggggattt tgtatggagg
     5461 caataacagc tttttgtcta gttttagtat cactgacgat aggagatata gtttcagcaa
     5521 aaactaaagc ttttgttccg tcggtttttg tatcagcaat tatatttata gtaggatttt
     5581 ggactttttt cccagaaaat attgtggact tagccgcttt aggaacacca ttagctcaat
     5641 taggtatgct tttgttaata actcatatgg gaactatgat gagtattaag gaattagcag
     5701 gtcaatggaa aacaatagta atagccttgg caggaatagt aggaatatgt gtaggtgctt
     5761 tggcattagg gacagtagta tttggatggg atacagcagt aatagctaca cctcctctta
     5821 ctggagggct agttgcatct ataatgatgg cagatgctgc tacagcaaaa ggtcttgcaa
     5881 gtttatcagt acttgcaatt ttgatgtatg ttgcacaagg atttgcgggt tatcctatta
     5941 cagcattaat gcttaaaaaa gaaggaaaaa gacttttatc agactttaga agtggaaaag
     6001 taactgtaaa tacagatgaa gaaaaagtta aggatttacc tgaacaaaaa agcagattta
     6061 agataatacc agacttacca gaaaaatatg atacgacata tatgataata cttagattag
     6121 gatttgtagc atggttagca gtacaattta caaatcttac aaatgaagtt gtatcaagat
     6181 atgttatatg tttaatattt ggtgtaatag catcagaact tggaatagtt gatagaaaac
     6241 cacttaataa atctggttca tttggatggt taatgacatc attgatggct tttatatttg
     6301 cagggttagc aaaagctaca ccagcaatgc ttgttgaaat ggcagttcct cttgtaggaa
     6361 tcataatatt tggtgtaata ggtatgggaa ttttctctat tatagtaggt aagcttttag
     6421 gtgaaagtaa agaaatggct ttttcagttt cgcttacatc actttatggt tttccaccaa
     6481 actatatatt aactgaagag gcagtaaagg ctttggctga aacaccagaa gaaaaagaat
     6541 ttttaatgga taaaatgctt ccaaagatgc ttgttggagg atttactaca gttacaatag
     6601 cttctgtagt aatagcaggt atctttgtaa atctaatata gtatttaatt gataaaaagt
     6661 attttaaata agaagaatgt gtactacttg gaggtataga atggatatta aagaaataac
     6721 aaagagttat aaagactatg taattaagct tagaagggaa tttcatgaaa atcctgagaa
     6781 aagtatggaa gaagttagaa catcaaagag agttaaagaa gaacttgata aaatagggat
     6841 accatatgta tcagctggag gtacaggtgt aattgcaact ataaaaggag ataatccagg
     6901 aaagactgta gcacttaggg gtgatatgga tgctctacaa gttgttgaat gcactgatgt
     6961 agaatacaaa tctaaaaatg aaggacttat gcatgcttgt ggtcatgatg gtcacacatc
     7021 aatgcttcta ggggcagcaa aagtgttaaa tgatattaag gatagtatca atggaactgt
     7081 aaaattattt tttcagccag gagaagaagt tggtaaaggt gcaagagcta tgattcaaga
     7141 tggtgctatg gaaggtgtag atagtgtgtt tggaatacat ctttggactg atgtagaatc
     7201 tgggaccata tcggtagaag aaggaccaag aatggcttct gctgatttct ttaaaattac
     7261 tgtaaaaggt agaggtggtc atggttcact tcctcatcaa ggtgttgatg cagtgcttgc
     7321 aagttcagca atagttatga atctacaatc tatggttagt agagaagtaa gtcctctgga
     7381 accattagtt gtgagtgttg gagttttaaa ttctggaact agatttaatg taattgcgag
     7441 tgaagctatc ttagaaggta caataaggct atttaatcca gagcttagaa agcaaattcc
     7501 tggaatttta gaacgtatag caaaatctac agcagaagca tatagagcag atgctgaatt
     7561 agagtatggg tacttaacac cagcagttat aaatgacaaa gaatgttcaa agatagctac
     7621 agatgcagca attaagctat ttggagaaga ctgtataaca ctatttgaaa aagtaacagg
     7681 agcagaagat ttagctgagt ttatgaatat agcacctggt gcactagcat ttgtaggagc
     7741 tagaaatgag tcaaaaggtg cttgttatcc tcatcatcat ggatgcttta atatagatga
     7801 agatgctcta gaaattggaa ctgctttata tgtgcaatat gcagtggatt tcttaaataa
     7861 ataattttaa tatactataa agaaaaagca gagactaagt ttatatctct gcttttttat
     7921 tgtgtatatt ttttataaat attgactata taaaatatat atgatagcat aaagaaaatg
     7981 ataatcaata tcaattaaaa agaaggtgat ttatttgaat aatgtaatat ttgcattttt
     8041 gataacatta ttggctggtt tatctactgg tataggaagt tgtatagcat tttttgctaa
     8101 aaaaacaaat actaagtttc tttctataag cttaggattt tctgctggtg ttatgattta
     8161 tgtttctatg atagaaattt ttcctaaagc acaagatgct ttgacaaaat ctatgggaga
     8221 gaaattagga agttggtcaa ctgtaatagc tttttttata ggtatggcta tcattgctgt
     8281 tattgataag ctaattccac aagaagaaaa tccacatgaa ataaagaaaa tggagaatat
     8341 agaagaaaag aatatcaaaa aaaataagtc tcttttaaga acaggtatat ttacagcaat
     8401 ggctatagca attcacaatt ttcctgaagg attagctaca tttatatcag ctcttgatga
     8461 tgttacaata gctattccga tagcaattgc aattgcaata cacaatatac cagaaggtat
     8521 atctgtatct gtaccagttt attatgccac aggagataaa aagaaagctt tttattattc
     8581 ctttttatct ggtatgtctg agcctttagg agcgattatt ggatatgtac ttcttagaaa
     8641 ttttttaaat gatataactc ttggaattgt atttgcaatt gtaggaggaa ttatggtatt
     8701 catatcacta gatgaattat tgccatcagc tagagagtac ggggaacatc atttatctat
     8761 ttatggattg attgcaggaa tgggtgttat ggctattagt ttattactat ttaaataact
     8821 tttaaatata tgattgtaac ctaaaattag tatcaaaatg atataattat ttaaatagat
     8881 ttttaagcta tttaaaaata tcattttgga gaatgagtgc tatggaaaag aaaataaaat
     8941 tcaaatttga ctggtatagt tcacctgata agatagagaa atggttagaa gatattgaag
     9001 ctaaaggata taatttatat aagataaata aatttggagg aatattttat tttcataagg
     9061 gaagtccaag aaaaataaaa tacactgttg attactacta tgatgtagag aataattatt
     9121 ttgatgaaca taaatcaaat ggctggaatc ttgtatttag gagtattggt agttttatgg
     9181 gtacatctgc atggtatata tggtcgaaag aatacaaaga catacctcct aaattatata
     9241 gtgattctga taaaacaaat aaagtaaagt caattttatt tatgaatatg tttatattct
     9301 ttatgttagt agtaatattt atatatttat tgaatattga aataagaaat ttatatagtg
     9361 gttatggagc atctgtaact gctattgtac tacagattat attgttgatt ttatatgcag
     9421 tacaattata taggagatta atgtattatt taaggcttaa aaagtttaaa taaataaaag
     9481 aaggtgtcct aaaattattt ttttaatatt ttaagacatc ttcttttgtc agtaagtact
     9541 gtttttaatt atagtaattt ctgtctttaa attgaaatat caaataatag ttgtatggga
     9601 ttgagtattg ataaaaataa tttaaaaatg ttatcctaaa gtatcagata tacaaaatta
     9661 tctttaaata tattataaat attttaattg tattaactag aaattatgta aggaggcatt
     9721 ttatgattaa gatagcagtt tgtgaagatg aaaaagaaac acaactttta atagaagatt
     9781 accttgagaa tatattaaaa gatataagta tagaatatga gatacaaaag tatatatctg
     9841 gagaagagtt actggaaagt aatttaaaag atatagatat attacttctt gatataaaaa
     9901 tggaaaaatt aaatggaatg gatactgcaa gaaagattag agaagtagac aatgaaatgg
     9961 aaataatatt tgtaacttca ttaatagatt atgtgcaaga aggttatgag gtaagagctt
    10021 ataggtattt actaaagcca atagagttgg aagaactaaa aaaacatgtg ctaacttgta
    10081 tcaaagatat tgaaataaat aaggaaagtc atattacaat aaaaaataag tctaatacat
    10141 ataagattta tttaaatgaa ataaaatata tagaagttca aaaaaaagat atgctaatac
    10201 acacaataaa caagaatttt gatataaaat atagtttagg caaaatagaa aaagaattaa
    10261 atccatataa atttataaga tgccataaaa gttttatagt aaatttaaga tatgttgaaa
    10321 atataaagcc taatactgca atactggaaa gtggagaaga ggtacctatt agtagatata
    10381 gatataaaga agttaaggag aaattcttaa aatttcttgg tgatacaata tgctaaactt
    10441 tatgactgag ctattagtag ttttatctat atttatacag ttttctataa tgaacggaat
    10501 tttaatttat aaaaaggata gtaaaaaaaa tgaaaaaata gtattctata ttgtcttgtt
    10561 tcttatttat attataattc agataagtag attatttaat ggaggaa
//
LOCUS       DAMJZE010000139        10582 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJZE010000139 DAMJZE010000000
VERSION     DAMJZE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.142"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            6..578
                     /locus_tag="PC541_002889"
     CDS             6..578
                     /locus_tag="PC541_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2840335.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(1591..2253)
                     /locus_tag="PC541_002890"
     CDS             complement(1591..2253)
                     /locus_tag="PC541_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840336.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2270..4825)
                     /locus_tag="PC541_002891"
     CDS             complement(2270..4825)
                     /locus_tag="PC541_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2840337.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(5065..5253)
                     /locus_tag="PC541_002892"
     CDS             complement(5065..5253)
                     /locus_tag="PC541_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2840338.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(5265..5444)
                     /locus_tag="PC541_002893"
     CDS             complement(5265..5444)
                     /locus_tag="PC541_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840339.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5449..5706)
                     /locus_tag="PC541_002894"
     CDS             complement(5449..5706)
                     /locus_tag="PC541_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840340.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5966..7359)
                     /locus_tag="PC541_002895"
                     /pseudo
     CDS             complement(5966..7359)
                     /locus_tag="PC541_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(7353..8028)
                     /locus_tag="PC541_002896"
                     /pseudo
     CDS             complement(7353..8028)
                     /locus_tag="PC541_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(8577..10106)
                     /locus_tag="PC541_002897"
     CDS             complement(8577..10106)
                     /locus_tag="PC541_002897"
                     /inference="COORDINATES: protein motif:HMM:NF012862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HDF2840341.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
BASE COUNT         3542 a         1554 c         1040 g         4446 t
ORIGIN      
        1 attaattgaa aaaactagta aaaatattaa taaatattat ttgcattatt acgctaattt
       61 tctctagcct ttcgatttac ataaaattat ctgaatataa aaaagctgat gaagtatata
      121 cggaactcag agaaaacact atcaataata gcaaacatca agaactttat gataaaaata
      181 atgattatag attctggctt aaaataaaca acgtaaatat agactatcct gtagtacaag
      241 gatataacaa tgatttctac ctaactcatg atttttataa aaattatctt cctttaggta
      301 gtattttcat ggattataga aataattttg aaaatgataa gagtttaata gtctatggtc
      361 attatatgaa aaataaaact atgtttggtc agctagaaaa ctatactgat gaagcatttt
      421 tcaaagaaaa taacttagta gaaataaatt ataaagtaca aacttatact tatgaaattt
      481 tttcagtata tactgctgac ttaattaaca gagattattt aagtatacac tttaatagta
      541 attatgagtt taaagattat ttgaattata taacctaaaa atcagtacac aaatctgata
      601 taaaggtaga tatttcagat aaaattataa ctttgtatac ttgtaactat gaatttaaag
      661 atgctcgtac tatagttcat gctaaattaa tttcaaaaaa agagactgat aagtaggttt
      721 gtgtaaatgc agacttacct acagtctttt ttgaacgtaa aattagtaag tatggaataa
      781 tattcatatt aaacttttgt aggcaaagaa atgaaaaatc aaagagaaat aattattaca
      841 gatttagatt accaagctga attaagaaag tgtaaaacta tggaggatgt agttggtgaa
      901 gatagtatat aatagtttaa cttataaatt atcttggtga tattaatgaa gtttcaaagt
      961 atatattatt tattgatata aataatatta aatctgttgc taacagatgt acctttgttt
     1021 ggagacaaac tatttaagaa atataactaa aatgttgaag tagctacctg actttatttt
     1081 gaaatgcgaa caagattttg gcattaagat aatttagaaa gaatcactgg tacacaagga
     1141 tgtgaactta gaatgaatag aagtattcag gcataaggag ctttcaccca aataaaacat
     1201 agtatgaact ttaaaagaat tctaatcaga ggcaatcaaa atgttctttc atagtttata
     1261 cctgtagtgt tagacatagt atttataagt ttcacaaata agataatcat taataaatgt
     1321 agtatgtata ttcatcctct caaaaatatt ttttaatctt acactttgta aattattcta
     1381 ttttaaaatg gtatttttat acctttattt cttatgaccg ttttcaagct caaattttgc
     1441 ataaatataa attaatgaaa agcaagtaaa aaaagaactg tcgcacagca atttaaaatt
     1501 actcattcga cagtccttat ttcgtggatt taaatagtgg agaagaatca caagaataat
     1561 acccacgatt aaacctttat acaatacatt ttatatgact ttaccatcac taatagttat
     1621 tatacaatca gccatcttag ctatttcatc attatgagtt atcataacca acgtctgctt
     1681 gtattcattt acacaatact ttagtaaatt catcacctca ttagttgttt tactgtctaa
     1741 atttccagta ggttcatctg caaaaattat tgatggctta ttggcaagtg atcttgctat
     1801 tgcaactctt tgttgttgac cacctgataa ttcattagga aatttattaa tctgactttc
     1861 taatcctaac ttagttatta aatcattaat ataattatta tctatctttc ctttagcaat
     1921 taaaatagga agaactatat tatcataaac attgataaca ggtattagat tatagttttg
     1981 gaatataaac ccaaattctt tacttctaag attagatagc ttttcatcac ttaaagtata
     2041 taaatctaaa ttatccataa atacattacc taaagttggt ttatcaagcc ctgccataca
     2101 gtgaagtaat gtgctttttc cagaaccact ttgtccaatt attgcactaa atttatgggg
     2161 ttctatctct aaagtaattc catcaacagc ctttatttca ttttctcctt ttccatatat
     2221 ttttttaata tctataactt ttaacatctt catacattta cctcccaaat tattcattct
     2281 cttttatacc ttcaataata ttcatacttt caaaatctct attacttaca actactgata
     2341 ttaaacatac aaatacagat atagcaaaga atagtaaaga ttccttaatt gggaatgtaa
     2401 aagacataaa attctctatt ccatagttta tatacgcatc attattccaa tttgtaaatt
     2461 taactagatt tataaatgat ggaatcatag ctattataaa gctaaaaact gcatatgcta
     2521 atgcttcata tataagcatt tttttcatat ttttaagact aacacctaat gctctaagcg
     2581 ttgaaatttc tttttttcta attagtagat ttgttcttat agtacagaaa atattaattg
     2641 atgctattat taaaactagt gcaattattg ctatttgtga tttaatgtaa cttttatata
     2701 tactttctga catttcatga taacttaatc tactattaaa tagagaccct ggtatacttc
     2761 ttgaaatttt ctgtatctca ttattaacaa tctcatcata tggattttct aaatttatgc
     2821 ctaattgagt gtatttttgt tctcctgtta tgtttttaga gtgaatatta cttgttataa
     2881 tttctagatc tggcataaaa tcgccatctc ccatagcaat ccattcatcc tttaaaatta
     2941 cagccactct taccttcatc tctttataaa caattgtatt attttcttca acaggaattt
     3001 ttacagttat tatgtctcct atatttaatt ctttgaatat aggctttata gtatgatcat
     3061 caactatatc ataaaaataa ttatatactg ctacatttgg atatccatca gtgtcctttt
     3121 taatatcatc taagctacct tcttcaacaa agtcatttaa cttttcatct atataatcat
     3181 catcataaag ctttaaaact aatttatctt ccattatctc ttttttttct actccattat
     3241 aggttgtaaa ttctttgttt atatccttat tattaaaagt aaacattcca tttgttattt
     3301 gaattttatg tatactttta acacccttaa ttttttctat attctctaca tcactatctg
     3361 tgtattttga tatttgcggg tctgcattta ctccaaattg cattgttata ttatatctat
     3421 tttgcatatc ttcaatttta ctgttgacct cttcttgcat acttgagaat gtagtaatga
     3481 atatatatcc tcctaaagca atagacaaca tagatatagc agctctaacc ttatttcttc
     3541 cgatattttt aagtcccata aatccataaa atccaaatat tttgcttact agtctgtcta
     3601 ttccaagtct acttctgtga tttttaattt tgtctgtatt tattattgat tcaataggtg
     3661 atatcttgcc gattttaaaa gttggtataa tacaagatat aaaaacagaa atcattccaa
     3721 ccaatatagc ttttataact atttcattat ttatatataa tttagggtta agaccagctt
     3781 cactgtataa aacttcgtta taattactaa ttcctatata tgaatatgta accccagcta
     3841 ctaacccaag tagtatacct attattgtta taattaatgc ttgatagatt atcatacacc
     3901 ttatgtttct tttctttgcg cccatcagtc taagcagtcc catttctttt actgattgat
     3961 ttagtgtaat attgaaaata ttaaatatga aaattataga tactgtagtt attaaaacat
     4021 ttcttttaaa aacattcgga tcattcttat catcttctac ataacgaagt tcccttacaa
     4081 gaggtacatt tggtagaaaa cttttttgtc ccaacttata ttttttcata agataatcac
     4141 attcaccttc aatatttctc catccacttt taaaacttaa tatactatta taactaatcg
     4201 catcatgagg tataatgtta tttgtttcat cattgccata agttactgct tcgtattgcc
     4261 aaaccacatc atagtaccct tttggttttt ccataactcc tactatttta aattctttat
     4321 ttatagaaaa taattgatta cttccgttag tatctttata gttctttttt attgttaaat
     4381 ttaatgtaga tcctactcct tcacttaaat tcattgcttc tagtgctttt ttctctaata
     4441 ctatttcatt gttgttttta ggtagtctac cttctattat tttgttgtat ctacttttta
     4501 gatactcttc gtttgatgaa gttaatataa atgagtttcc ttttttatct gaaatacgtc
     4561 ctagattttg taccgaatta ccctttgata ctttttcatc agtttctaat tttttagcat
     4621 catcaagtgt caaatcacta aactctacat ggtatccact gtaaagttca tgtattgcta
     4681 ctacattgtt ttcattttgt gaatctttta taacactagg tccaaatatc aacataacac
     4741 ctaacataac tcctattatt aaaagtaatg ttctaagttt atttttcttt aaatatgcta
     4801 gtgacatttt taagtaaatc cccatataat cctcctattt tactataaat tatttatcca
     4861 actcatctgt taagtagatt ggtggatatt ttaaatcttt taattcttca ttattttttg
     4921 aagcttacta ccatcttcct catgataaac ttgcagtatc accttattta catgtagtgc
     4981 ataataatca ccattataaa aatattagta tttataacct ggataatttt tgtaaaatga
     5041 tttataaagt atcccatcct aattctattt tgtatacttt tcttttgtaa tacgctctat
     5101 atcatcacct gaaactcctt gatatgtcat atatttatct atatattttt cagcttcatt
     5161 ccattttcta aaatagttgt tgtatataat aaatcctatt atggctataa tgcttataat
     5221 taaagttaat aaaatagctt tgtattttct catagtgaat cttcctactt caatttaaat
     5281 tctttaataa gttttaattc taaagactta gttttatcat caaatttctt aacttgaatt
     5341 tttatatctc ccaaattatc aacctctaca tttacttcac ctttatcaaa tacttcttca
     5401 atttttctaa aaaattcttt ctcacttatt tctatatgaa tcaatgatct atatgttata
     5461 aatggccatt atgaatttat catcttctga cagcttttca ttataagaaa aatctttaac
     5521 acattgaatt gataatattt ccttcgtatc cttcttttcc ttagttattg aataatgtaa
     5581 aacttggcta ggttgttctt ttgtatactc caagtttccc aaagacaaaa caaaacttcc
     5641 atctgattta ttttcagcta ccctaagacc actattttct attgattctg atattttctt
     5701 tgacaagtct tcttgatact agaaatctgt tcaatagtat atattttttc atttgtacct
     5761 gaaaccttat ttgtgcatcc tgttattaaa cctagtgtta agcttatagc tataaccata
     5821 gttattgtaa tttttttatc ttcatataat cctccttata tattgaaaac tcgttatttc
     5881 tcataaaaaa aagacgctat ctttttctat taaaagataa catccctata tgtttagtat
     5941 caatatatgt tccccaactg gtaggttaat cgacccaact atcaaaattt tgatttaatg
     6001 gtatataaat attcaatata aatttgtctt ttttatcttc aaatgataaa tctcctctat
     6061 atttttttat agaagatttg atgctttaaa tccctattcc atgtacaagt ttatcttttt
     6121 tatctgttaa taataaactt ttattctttc taattgaatt tattttacta ttctcacatt
     6181 taattacgaa ataggacttt gagatagttc cttttatttg tatatattta tcaatacttt
     6241 tatcatgtat cttatcgcaa gcttctattg cattatctaa gtatttgcaa atatattaga
     6301 tacatctatt ggttttataa aattcacttt ggaaaaattg atatcacaaa tgaatttaat
     6361 ttttcttttt tcacttacat cacttttttc atttattatt atatccaata taacattacc
     6421 tgtattgtaa attgatttaa attctttaat ttaatctttt aaactattta catacccatc
     6481 aattttcctt ttttatattt gagattttct atacaatata tatgattatt tatatcatga
     6541 taaagcttct taactttagc gtgtgattct tgaatactta gataatgaga atactgcata
     6601 tctaacttat tttttattac ttcattttgc aattttaact tattttcctt aattattttt
     6661 ataaacacta taataatgac taagctacta gctagtatta acctaggtat cacaatatac
     6721 ccaactatat atatttccta atacccacca aatcgataca tttttctatt tatataaatt
     6781 attaatatag taaataaatt tgttattaaa gcaacagata agtatatata gtttatttta
     6841 ttatctttat ctagtcccaa tcttttatta ataaatttaa atatagcaca agctactata
     6901 taactattat tataagataa tagtatatat ctactttagc attatcataa ctaaacacta
     6961 atagagcttc ctctgatgat attacattac ttgtaactac aaaaaataaa tttatagata
     7021 taacatcttt tattaaaggg tgatatacgt caaataagaa aaatgaaact atatatttaa
     7081 caattcctat cttataaaaa taattatagt aagttcccaa gtaataaata tcaaataata
     7141 gattatatca tatagatttt tcttcatatt ataatcatgg gtcttatcaa cattaaataa
     7201 tataatgatc ttagttataa gataagctaa taatacagta aaaaaagata ttttcttagt
     7261 cttatctgta tctttatcgt aagttaatat actttttagt atcattattg gtgtaaacaa
     7321 tgataatatt aataatattt tatttatatg gtttaacata ttctatcacc aataaatttt
     7381 agaaacccct cttttatatc tttatatcta tatctactaa taggtacttc ttcttgactg
     7441 tctaatattg cagtatttga ctttatattt tctacatagt ttaagttagt tatataactt
     7501 ttatgacatc ttacaagttc ctctatattt aaatcttttt ctattttttc taggatacat
     7561 atagcatcaa gagttttatt ttttgtatgt attagcatgt tctttttttg aacttctata
     7621 tatttttttg ctagattaga ttttgtatgt gttagattta tttttatttt tttcaatttt
     7681 tttggtacat gttaatatgt gtttttctag ttcttgaaaa tttattggct ttaataagta
     7741 tcaataagct cttacttcat atctttcctg tacataattt attagagatg ttataaatat
     7801 tattatctac ttttcttatg tttcttgcga tatctatact atttagttct gccattttta
     7861 tatctaagag tagtgtatcc tctcaattaa ctttgtaatg attcttcttt tgagctatat
     7921 ttttttaatt cgatattctt tgtttttatt ttttaatata ttatcaactt gattttttat
     7981 tacgatttgt atttcttttt catcatcaca agctgttatt gtaatgataa cctctcctaa
     8041 tattgtattt ttatttagat tcttcacttt aggttaaaac tttttaaatg tatttatcaa
     8101 taatatttaa attaaaaatg tataaaaata cctatatcca attggatata ggtattcgtg
     8161 gatttcaatg gtggagacga ggggagtcta taagtggtta tttgattgtt ttgttgtttt
     8221 ttgcaagtgt aggtatatag ctatttagtt atgatgttaa actgattttt tcggatattt
     8281 tttattgttt atttttgtag ttggggtatt attggggggg gggtggatga tataatcttt
     8341 taattttata aaagttccat taacagcata ttatacgttt ttaaatattt actagacttt
     8401 ttcaaaataa atatctaatt atttaaatca ataccaatat aatttataaa tggatttata
     8461 attgtatctt ctatcatgtt tagtgtaaac aacttatttt aatttaaatt cgtaaccaac
     8521 aaagtaccaa tctgacttat aattctatat tagtacaact atggaaaaat ttaattctat
     8581 atctcctcac ttaatcttat gacttctctt aaatctttgt aataatcctc tatattcatt
     8641 tccatacatt gctttctatt ttcctcagtt ctactatgat cccaataaac tccaaaattt
     8701 tgtatagttg acactaagtt tatacattta tcaaaccaat ctttaatttc aattggtgga
     8761 aaatctaaat ttataacttt attgtaacac tcatccattt ttttctttct actatctata
     8821 atttgaaaaa tattatcttc acatattttc ttattttcat attcattata gaaggatata
     8881 atttcttcag atatgtttat catatttgtt aaaatttctt taacttcgta tactgccttt
     8941 tctttagtga ttgtattttg acttatatat gattttaagc tttcgtagtt atcatatgtt
     9001 tcagtgtgaa caccgttgat aaaaaattta tttttcccat ttacactata ccatatatct
     9061 ttctcttgat atttatcaat ccaagtagtt ttacacatat atattccatt tatcatatca
     9121 atttcatctt tgccaaaata tacccatatt atattagcat cttttccttt taatctatct
     9181 tctcctattt cactattata aacttcaaca gacttaacta tgtcaaaaac ctccttagat
     9241 atttctgata tatcacaata ggagaattta ttttttaata taacttctac tgaatatctt
     9301 tttattatac ttatatgtaa agatttgaca ttgtatatta tgtaatcgtc atgctcttga
     9361 aaaactttct tttttatata agatttataa tcttttacat taaaatatat atctcccagt
     9421 atgttagacc taaagatagg attttgttta cagtttatat aatttttgtt caatatactc
     9481 atacgcaaat taatatactt gcttattgct cttaaagata actccccttt tcttactatg
     9541 tattcatttc ttaacgcttc tgataataaa cttgtaaatc tactaattga actatctgga
     9601 agagaaaatg attcttgata ttcgctactt gatactaaca ctgcacatcc ttttctatca
     9661 aacaaggaaa atatgtcttt aatatttatt ttttgtttgt cacttacatt aaatttacct
     9721 gcaaagcaac aatcaataaa tacaatttta tttttacaat ttaatccttc tataaaatca
     9781 attacaaatt gaatagaaac taattcatca cttaatacta agtaatgcac tccgttatta
     9841 catatgcatc catgccctga aaagtaaaat ataaatgtat catcatctcc tatagaatcg
     9901 cttatttctt gaaaagtttt aaaaaataaa tcttttctga cttcatactc catacctaaa
     9961 gttcttatat tattcgaagt aatccccagt cttttatgta acacattatt aatttcaatt
    10021 atatcatttt tacaaaatag taaatcttta tttttaacta tactatattt acttactcct
    10081 acaattaatg cttttaagct tttcatattc cattccccct ttataatttt tcaatttaat
    10141 atacttaaaa tgatgtttta attgttttat tgattttata aaagctagta tcacactact
    10201 tttttgactt tttcattatt tacttttgta gttggattat aatttttcaa ggtaagtata
    10261 tatatattta tttacatcgc acccatataa tttatatctc tgatttatct gacctgaact
    10321 tagtgtaatt taaaccaatg taaaatcatc tatctcttca ccatatattt tttaaaattc
    10381 cccaaaatat tgatgttaat agaatttcat gtgttttttg tattctacta tccataagaa
    10441 ttttaaagtg agtaaatatt tccaatcatt tatatcttat ttaatatcat tttattccct
    10501 caagtattta tttaaattgt tatgctttat ttataaaatt atacatacat ttctaaagtt
    10561 agacatacat ctttaaaaac ct
//
LOCUS       DAMJZE010000140        10581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJZE010000140 DAMJZE010000000
VERSION     DAMJZE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.143"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(278..535)
                     /locus_tag="PC541_002898"
     CDS             complement(278..535)
                     /locus_tag="PC541_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2840342.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1115..1336
                     /locus_tag="PC541_002899"
     CDS             1115..1336
                     /locus_tag="PC541_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2840343.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2195..2425
                     /locus_tag="PC541_002900"
     CDS             2195..2425
                     /locus_tag="PC541_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2840344.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2438..2587
                     /locus_tag="PC541_002901"
     CDS             2438..2587
                     /locus_tag="PC541_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2840345.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2844..3281
                     /locus_tag="PC541_002902"
     CDS             2844..3281
                     /locus_tag="PC541_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2840346.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            3538..4431
                     /locus_tag="PC541_002903"
     CDS             3538..4431
                     /locus_tag="PC541_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2840347.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            4609..5433
                     /locus_tag="PC541_002904"
     CDS             4609..5433
                     /locus_tag="PC541_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2840348.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            5532..6140
                     /locus_tag="PC541_002905"
     CDS             5532..6140
                     /locus_tag="PC541_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2840349.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            6244..6420
                     /locus_tag="PC541_002906"
     CDS             6244..6420
                     /locus_tag="PC541_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840350.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            7686..8438
                     /locus_tag="PC541_002907"
     CDS             7686..8438
                     /locus_tag="PC541_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840351.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            8428..10539
                     /locus_tag="PC541_002908"
     CDS             8428..10539
                     /locus_tag="PC541_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840352.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         4063 a         1381 c         1313 g         3824 t
ORIGIN      
        1 tttatatagt ttatttattt atttttcccc tttatacaag caattgtatc attttatcta
       61 ttttttagcc aacaaatatt ttcgaccatt ttctttgtat tctcaatata tctccagtat
      121 ttaacaaatt tttttataga atatacaatg tatttaaatt aataagtagt aaatttagtt
      181 aatttgtcta aaaaaacttt ataaaacatc ttctttgtct ttactcttac gtttaacaag
      241 gctattttca ttatgtttaa acattatttt attatattta aacactattt tcattgagta
      301 ttcgcttaat attgcttaca cacatattat atctaatagc caattcattg tggttgactc
      361 cattaaattg ttttactata tccctatttc tagcatatct aaaaagcccc ttatacgtag
      421 gtatataagt atttgttcct ccatacaact tggcaacctc tatgaactta tcaattccga
      481 ttatttctac cattgcatga aattgaactg gtatatcctc cacttttatc ttcataagtt
      541 acacctcctt taagatattt ttatagactt aacaaagttt ttatttaata cattattgac
      601 taaactatat ttattagttt tattgaatct aataaaaaat ttcttagctt taaaattaat
      661 ttttgtatta acttcaataa aatttctcat aacttataac tttgttagct ttaataaaga
      721 ttttaatggt ttaaaagtaa aatgttaata ttggactttt aactttttta tttacagatt
      781 aaatttaata aaatttttaa cataagcttt aacatttaat ttaacataaa ctttagtacc
      841 taacatttaa ctctacacat atgaaaatcc atacaaaact atctcaacta agcatgaata
      901 tatacaaata ctatttcaaa acactaagaa catatcttca caaataactt gtaaactttt
      961 tcatcagcaa aaaagtaaaa tttttcctat aaaaatttac acaaaaaatc tttagaaatt
     1021 actgtttaac tccgatatta tcttagatga tagtattata gctaacaaag aaagtcgaca
     1081 aatctttgaa attaaatttt taggaggtaa ttttatggaa gttgtttcaa caaaaaattt
     1141 atctagtata aaactaaaat tggatgctgg ttttgatgat aagggtaagg cagttgttaa
     1201 aagtaaatca tttgcaaatg taaaagcaga ggcattaaat gaagatgttt atgctgttgc
     1261 agaagcaata gcaagtcttc aagagaaccc tattgtagat attttaaaat tagatagtac
     1321 atcactatct aaatagtgtc ttttaaacgt actctgcttt acaaattaaa aaattaactc
     1381 tttatataaa agattaagtt atataatgca cttaatacgc tatagatttg gcagattata
     1441 acttcaattt tggttataac tttaatatat taacgctttg aaaaattaat atattaaacg
     1501 tatttcaact taaacatgtt agctatttta gatgctaccc tactttaatc taattaattg
     1561 aaataaatta aatattttct atatcagcaa taactagagt caacaattaa aatactaata
     1621 ttgacaattg aaacactgat actaacaagc aaaacactaa tattgacaat tgaaatacta
     1681 atactaacaa gcaaaacatt aatattgaca attgaaatac taatactaac aagcaaaaca
     1741 ctaatattga caattgaaat actaatacta acaagcaaaa cactaatatt gacaattgaa
     1801 acactgatac taacaaacaa aacactaata ttgacaattg aaatactaat actaacaagc
     1861 aaaacactaa tattgacaat tgaaatatta atactaacaa gcaagacact aatattgaca
     1921 attgaaatat taatactaac aagcaaaaca ctaatattga caattgaaat attaatacta
     1981 acaagcaaaa cactaatatt gacaattgaa acactgatac taacaagcaa gacactaata
     2041 ttgacaattg aaatattaat actaacaagc aaaacattaa tattgacaat tgaaatatta
     2101 atactaacaa gcaaaacatt aatattgata attaaaatac caatattaac aagcaaacaa
     2161 ttaaaatata aattattata tcggaggtaa ttatatggaa attaagaaaa aattagtcat
     2221 gagctttaaa aatgtaaagg ataaacaagt ttctttttct atagaaaatc caaaggatga
     2281 tttaactgaa gaaaatataa aatctgtcat ggatttgata gtatctaaaa atattttttc
     2341 ggttggagga tttgacctag cttctgttgt tgaagccaag gttgtggaaa caaataccac
     2401 tccttatgac ttagttatag aatagggggc taattttatg gatgatagtt tacaactgtt
     2461 aataaccaat gttggttttc ctatagctct atccatgtac ttgttggtta gaattgaggg
     2521 taaattaaat actttatcaa acagtattga tacattatcc aaaaatattt tgatatttaa
     2581 gaagtaattt gacaatatat gtaagtcata aaataggtct atttgtaaaa taattagaca
     2641 tattctatgt gaaatactag gatatttgga taatttatcc aaatatccat agtatttttt
     2701 tgaattttac cacctcaaat ttgaatttca ctgttttatt tttgaatttc actatgctta
     2761 atttgaaatt tagaacgcat ctagttggac tcaataagat ataatgctat tctaatgaca
     2821 gtaataaaat gaggtgatga ttaatgaaaa tacatgtcga acaaggtatt aattgtgatg
     2881 atattgaaat aaccataaaa tgttctgctc tagataataa cattgaaaaa ataatttcat
     2941 tattgagtat accgttactt gatataaaag gtaaaaaatc aggagagatt tataaactca
     3001 atgtctgtga tattttatac tttgagacag ttggaaataa aacatttgct tattatgaag
     3061 atgatgtatt tgaaattgat ttaaaaatat ataaattatc aaaattatta gctaatacat
     3121 ctttctcacg aatatctaaa tccatgattt taaatatgga ctacataaaa agtataaaag
     3181 taatatcaca taatcgcatg caagctactt taaaaaacaa cgaaaaagtt attataaatc
     3241 gtcgttatat aaaaaatatt aaagaaaagc taaacattta ataaaaatct aaagtaattt
     3301 attaatatca ctaattgcaa atttattgac ctatttaata attaataata atataatata
     3361 ttcaatatta aatactaatt aattatattt agtgtaaaaa taattaatca aaatataaaa
     3421 aataggatat tattataggc aataactaac atcaagcgat aacttaatac atataaaaat
     3481 aaataaacaa ttaataaaaa ttaaaacttc attataaaac ggaaggatag tgaaaaaatg
     3541 caactatata acaccccctt taatacaaac aaacacttaa tctatttctt tacatttact
     3601 ctagtattga actggatatt cagtcttaca cctgcgttgc tcggaattac aaatacaata
     3661 cttggagaaa tcatttttta tcttgcatat acatcaccag ctattgtagg tctatttttt
     3721 atttctacaa tctatccaca aagtgcaaaa caagatttta tttatagata cctaagcttt
     3781 aaagaaatcg gaataaaatg gatactatta acaatattat tttttgcttt aacttttatt
     3841 acttcgttaa taatcggaaa ttactttaat gtaaaaactt ctgatataga ttggatacac
     3901 atagcagtgt ttagaccttc taaaatagct tatatgctat tagcaagttt tatattgagt
     3961 atacttagtc aagaagctgg atggagagga tatgccattg acaaattatt agttagattt
     4021 ggatttatag gctcttcaat tattttaggg cttgtttgtg gaatctggta tctagggtcg
     4081 tattttacac ctaatcaaat accatataat ttggcacaat actccctatt tgatgctttt
     4141 ttatttatac caaacattgt acttttaaac tttataatta attttgtata tatcaataca
     4201 agtagaagca ttttatcagc tggattagtt catatgatgt actatttttt caacattcaa
     4261 ttacttctac actaccctat taaattatgt gtaatagctc aatatgtaca aataatattt
     4321 ggtttaatat ttcttgtata tatgatttct tctaataaat ttaaacagaa ggtaaattct
     4381 gaaattgaac agataaagtc ggatgatttt gaatttgagt tagactggta attttaatat
     4441 gaattataaa ttaagataat ctatgatttg atacgattac tacttaaata taatttactc
     4501 tctataattt attgcaacat ctggaaaata attgtatttc cacaaacgct tgaagtatat
     4561 aaaaacaaaa tatatactga ttataagtga aaaggaggtc atattttcat gtttaaaata
     4621 ggagaggttt caaaactaac acaaatttct attcgtatgt tgcgttatta tgatgaactt
     4681 ggtattttaa aacctgcaaa aacagacaaa tatacaggtc atagattata ctcagttgaa
     4741 cagatttcaa tattacaaag aattgtatta ttgagagatt caaaatttag cgttgcagaa
     4801 atagcaaata tcgtacataa ttggaatgat gaatttgtaa ttaaggaatt gaatagaaaa
     4861 aagaatgaaa tacaaaagga aataaagcag gaacaacaaa gaataaataa aattgataaa
     4921 tttatagaag ctataaactg tgataaagat gaaattcatt acaatgttgt ctttaaaaag
     4981 attccaagtt ataaaatcat atcacttaga gagataattc cagactacca atctgaaggt
     5041 atactctggg aaaagctatc taaatttatc aaagaagaac atatagaagt atcacgtcaa
     5101 tccaacaata acatagcttt ttaccatgat gaagaagtca aagataatgg tgtagatatt
     5161 gaagttggaa tggttgtaaa gaagattgga aagaacaaat ctggatttat atatcgtgag
     5221 acagaagaaa ttgatatgat ggcatgtact atggtttatg gtccatatga aaatatagct
     5281 ggtgcatatg aatcgttctg ttattggtta gataaaaata gtgattatca aatatcaggt
     5341 ataaatagac aaatcggaca taaaggtgaa cataatgaaa taaatcctga aaattatctt
     5401 acagaaattc aaatccccct tataaaagtc taatagatta gtacttaaat acttaagtac
     5461 taatcaccca ttgacatccc aaaattttca tgatagtatc taaacgtgta ttaaacatgt
     5521 ttaatttttt tatggagaaa cttttaaggg gaaaaattat gaaagcagat tcaacaaagg
     5581 aacaattaat taatgccaca atccagttac tatcttcaca tagagatgtt tcaagcatta
     5641 cggctagaga aattgtagct aaagcaaatg tcaatttagc tatgattaat tattatttta
     5701 aatctaaaga ggagttaatt aatatttcta ttaaaaaatt tttaaagcgt catatggatg
     5761 aatataaggc tacacatgat actaaaaatt tagcacctaa acagcaatta agggaaatac
     5821 taattgcatt ttttgatttt ataggtgaac atagtcaatt tataaagatt tctgtaccct
     5881 atatattgat acaggaagaa attgtttatc catttgagat actaccttta attaaggctc
     5941 actatgaaaa tcagaataac aaaaaaactg aagctgaata cagaactatt gcgtatcaaa
     6001 taacttcatt tattcagttg ctttttcttc gttctaatgc ttttttcaag tatagtggta
     6061 ttgatattct taatattgaa cagagaaatg gtttaataga ttttcaatta gatttattat
     6121 tggtagatat tacaaaataa tactatatta attttttatg tacatttttt tattttatgg
     6181 taacgtttga aaatattctc attgactaaa aatacttgca ttaaataagg gggaggaatt
     6241 ttaatgtttt ttacatttat aggagtaata tcagcaattt tattgttgtg tacaattata
     6301 tgtggtttat ggataaaact taataatcgt aataatgact ctaattctca ctttactttt
     6361 tcaatacttt caattgcttt ttcactgtta attgtggtgg cctttttagt aagaaattaa
     6421 tgtctttgtt atgctgaggt ttattattat agatttttat tattgcatga tttttactat
     6481 attatagtat ttttattata ttacagggtc ttatttatta tagtgttttg ttgttacagc
     6541 attttattat attacagagt cctatttatt ataggatttt tattatattg taaaatctta
     6601 tttattatag gattttgtta tattacagac ttttattata ttgaaatctc ttatttattg
     6661 cagtgtttct attatattgc aaactcttat ttattatggt gttttgttat tgcaacattt
     6721 ctattatatt gtaaagtctt atttattaca gttctttatt atactacaga cctttattat
     6781 tacagcattt ctattacatt gtaaagtctt atttattaca gttctttatt acactacaga
     6841 cttttattat tacaatctct tatttattac agttctttgc tatactacag acctttatta
     6901 ttacagcatt tctattacat tgcaaagtct tatttattac agttctttat tatactacag
     6961 acttttatta ttacagcatt tctattacat tgcaaagtct tatttattac agttctttat
     7021 tatactacaa acctttatta ttacaacatt ttattatttt aacttcttta ctataatata
     7081 gtaatatttt aatatctcaa aatcatatca atcacttttc agcatagtct aaaatacttc
     7141 cactttctaa aattatctat catacctata ctaataatac tttttaacct ttttattata
     7201 catttcaatt gtttctaaca acctatttat ttccaaaact ccaatatatt ttctcaatta
     7261 cattgtgtta atagccccta aatcctttca aattatttac aatacattcc attaaataat
     7321 cttactgctc tataattctt tttttctttt taaccctact atacattatt tacatcattt
     7381 ttttcaatac aacattcgtt attctataac ctttttactt ttactatata cttcttttct
     7441 aattacacct tcaatgttcc acatcatatt actttagcac aacaattaat acctaaaaat
     7501 aaccctctaa atcctccaat aatataatca atttaactat aataataacc tctcacacta
     7561 tccaaacaat atattttcaa acattacaaa aaaacatcca actattacaa tatagctcat
     7621 atccctcctc ccctttcaaa tgctatcatt gacttacaag ataaatgaaa gtgaggatga
     7681 tatatatgct taaagtaaac caattatgta aatcatataa aacaggcagt aaaacatatc
     7741 ctgtattaaa aaatatttct tttgaaatca acaaaggaga atttgtcgca atcatgggtt
     7801 catcaggaag tggaaaaact actcttttaa attgtatttc atgttttatc ccaattgata
     7861 atggggaaat aattcttgga aataataata taagaaattt aaaagaaaag gaactttcag
     7921 aagttagaaa tcaacaacta ggatttgtat tccaagactt catgctattt gatggtctta
     7981 gtgtctttga aaatatatgt gttccacaaa ttatcaaagg tagtcaagtc ttacaaatgg
     8041 aaaaccgtgc aattaatcta tgtgagctat ttggtattga acatatcaga aaaaaatacc
     8101 ctgccgaaat atctggtgga gaaaaacagc gtacagcagt agcaagagca ctcatgaaca
     8161 acccagattt aatattagca gatgaaccaa ctggtaacct tgatagtaag tcttgtcgta
     8221 cagtcattga atcattttta aaagcaaagc aagaattaga tgctactata ctcatggtta
     8281 cacacgatag ctttgcagca agcttctgtg accgagttat tgtgcttaaa gatggaataa
     8341 tttataaaga agttgtaaaa tcaaattcaa gacataaatt cctagatgaa ttactagaag
     8401 ttttaaaaga aatgggaggc gctcaagatg acaattaata aattatacag taaacttcga
     8461 gcctataatt ggaagaacta tacaacttta ttcttttgta taatactttc tgtggtatta
     8521 gtaacttcat atgctctaat ttattttagc ccaacaatac aaaacatact tccaactggt
     8581 ggagatagca gaaaacaggc tacaatgata ttctcagttg ctatattagg atgcgctgtc
     8641 tttactacat acgcttcttc cctcttttta aaatttaaaa gcaaggaaat tggtattttc
     8701 atggctctag gaacagataa gaagaaatta aaaaaacttc tattttcaga gatattgtta
     8761 atttcaatag tatgctgttt tttaggtctt atattaagta ttcctgtatc ttttggtata
     8821 tggaaaatat ttcaattagt gattattgat accagagaaa tgacatatca ttttggaact
     8881 caaggattca tatatggatt tatatttagt gtatttgtga cattatgcat atttatactt
     8941 tctgtaaaat ttattaatcg tacaaatata attgatatat taaatgacca gagaatatct
     9001 gaaccaatac aggatgtaaa ggactggtat ggtatattag gtataatttt tattatactc
     9061 ggtatttttc taggatatct cgtaccttca ctaactataa acaaattaaa ttataaacta
     9121 ccatctatat ggagctttac ttatctactc tctcttattg gaatatatat gattatgaca
     9181 tacatagttg tacatgctaa aagaggaaaa catccagaga aatactataa aaacatcata
     9241 tcaactagca tgatgagatt tatgggaaga caaacagtaa aaaacatgtg tgttatttca
     9301 tttttgattg caggagcatt atttgctgta ttctatgttc ctaatatggt tacaggtatc
     9361 tatgatactc ttaaaaataa ccctatagat tatactttta cctacaatca aagagataaa
     9421 caggtatcaa aaaaagatat tgaacaatta gcagataaac acaatataaa aattcaaaac
     9481 tatatggaaa ttccttctat agaattaatt gttgatggtg tagaatcaat atatcataaa
     9541 gatggaacta tcgaaaaggt atatatgaaa aaaagctcct atgatatgtt ctttaattcc
     9601 aaagattttt ccaagctaat tggcaaacca ataactataa aaaatggtga atatcttgct
     9661 gttgtaaata aaggaaatga aaatcaagat tataaatata ccaaatttat atcaagccca
     9721 gtcacagaaa aagaaatccc ctttagatat gctggaaaaa taatatttaa tggttctttt
     9781 tttcaaaata gaagtatgaa cgcctatgtc cttaatgagg aggattataa atcatttact
     9841 aaagatttat ccataaaaaa ttacaataat tctatactat ttaatgtaga taaagatacc
     9901 tatgcttttg ccaatgactt aaagaaagaa attataaaaa gaacatctaa ggaaatgggc
     9961 atgttcagtg gttatgatga atacgtaaaa aaacgttatg aatcaactgg aagaaaatat
    10021 tttatggatg aagaatatcc aagtggtgaa ggtcatttaa aactagaccc aaacaacaat
    10081 cagttgtatc ttaattggaa atactatcct aactttaaaa ttttacaaag ccaagatatg
    10141 ataaaaaaca tggcagtctt cttaatgtta ttcatatatg tggcaatcat atgttttaca
    10201 gcagtaggaa tcattgcata tacaaggggt gttactatag ctataaatca caagcaggtc
    10261 tttatggatt taaatcgatt aggagcaaac aatgattata taaagttctg tattagaaat
    10321 caattaaaga aagtattttc ataccctctt attgcaggaa gttttttaat ttatctgttt
    10381 aattttatga ttatgttaaa taatgatggt agaatcgtca atagtgaggt caatgctctt
    10441 ttgataaact taggttttat agggatttgt ggggtttata tgtaccttat atatctatta
    10501 acttataaga aattcaaaaa aattattggt attttataat aaaaaaggtt attagatttt
    10561 taaagtaata aaatcattgg t
//
LOCUS       DAMJZE010000141        10535 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJZE010000141 DAMJZE010000000
VERSION     DAMJZE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.144"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            156..773
                     /locus_tag="PC541_002909"
     CDS             156..773
                     /locus_tag="PC541_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2840353.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(930..1382)
                     /locus_tag="PC541_002910"
     CDS             complement(930..1382)
                     /locus_tag="PC541_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF2840354.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1830..3188
                     /locus_tag="PC541_002911"
     CDS             1830..3188
                     /locus_tag="PC541_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2840355.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3461..3841
                     /locus_tag="PC541_002912"
     CDS             3461..3841
                     /locus_tag="PC541_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2840356.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3966..4166
                     /locus_tag="PC541_002913"
     CDS             3966..4166
                     /locus_tag="PC541_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840357.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4362..5090
                     /locus_tag="PC541_002914"
     CDS             4362..5090
                     /locus_tag="PC541_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2840358.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5112..6029
                     /locus_tag="PC541_002915"
     CDS             5112..6029
                     /locus_tag="PC541_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2840359.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6171..7355
                     /locus_tag="PC541_002916"
     CDS             6171..7355
                     /locus_tag="PC541_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2840360.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7462..7650
                     /locus_tag="PC541_002917"
     CDS             7462..7650
                     /locus_tag="PC541_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2840361.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7799..8449
                     /locus_tag="PC541_002918"
     CDS             7799..8449
                     /locus_tag="PC541_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2840362.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            8552..9490
                     /locus_tag="PC541_002919"
     CDS             8552..9490
                     /locus_tag="PC541_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2840363.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9516..10367
                     /locus_tag="PC541_002920"
     CDS             9516..10367
                     /locus_tag="PC541_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2840364.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         4288 a         1002 c         1855 g         3390 t
ORIGIN      
        1 aaagttgtat atttttttaa ggatataaag taaaaaaata tacaatatca agaaaaaaag
       61 atattgggta tttaaagtaa tactttattt aattttaagt aaatcgggta tctacatata
      121 tagatactaa tttcaaagaa taggagggtg tttatatgga aatattcgca ataggacatt
      181 caaattatcc atatgataag cttattaata tgataaaaaa atatgacata aattgtgtag
      241 ttgacattag agaaactcca tattctaaat ataatattca atacaataaa gaggcattta
      301 acgaatctct tagaaattcc ggatttttat atatatacat gggaaaagaa tttggagcta
      361 aaagaactaa taaagatgta tatacgcaag aaggttatgc agattttgag aaagtagcaa
      421 aagaagatat ttttttaaac ggaatagaaa gattaaaaaa gggttgtcaa atgggttata
      481 ggatagtttt attaggagct atgcaagaac ctataagatg tcatagaagt atcttggtgg
      541 gaaaagtatt aaataaagaa ggttttgatg taaaatatat aatgcatgaa ggaaatttgg
      601 catatcagga agatatagag gaatcattat tggataagta ttttagtgat agaaagcaac
      661 tgagtattga caatttatta gggagtgcat taacgagaga agaaatgata caagaaggtt
      721 ataagctagc taataaagaa ataggatata gaactgagaa actaggtaaa taaaaatata
      781 caaatagata aaatacagta cttatcttaa gtaaaataat attatgaatt tataatagct
      841 aggcatgatg tgccaattaa aaatattcta gttatttaat aatatcatta aatttagggt
      901 attttttaat agcacatcat gttgcagttt taaaaaataa aatctctaat attgccataa
      961 tcaactgttc tattaagagt aggatgacta gatataaatc taactgtaat ttttgaattt
     1021 aacaaacaag gatttgaaat taaaaatact ctatctccat tgacaataga agtaatagct
     1081 actggcaaaa tatctacaaa tgtatcatat ctgtttttag caatttgata aggataattt
     1141 cctacaaaaa caaatagatt taaatcatat atattatcag taaaccaagc ccattgacca
     1201 taaactttgt caatattcat tgtgcctaaa ccaagtgtat caaagtatgt tgctatagtt
     1261 aaataaatat tttccatgtt ttctaaatat gaaacagtat atcttctgaa acgtatgggt
     1321 gctgtggaag aaataggata aaccatattt acatttagat tatttgcatt taaattaacc
     1381 aaagtatcac atcctaaaat tagtctataa aatacaatat ttaaatggat gcgattttgt
     1441 tacatctaaa gtgtaatatt ttatatattg aaaaagtggt aaaagtttgg atgattaaat
     1501 aatattataa aaaatatata aaatgtactt attgttattg ctacacgata gtgatacaat
     1561 attaacgaaa tatagctact tttaataata acggaaaata tttatattaa attaataaat
     1621 attttaaatg tggtatccat aaaaaataat aaacataaga taattataaa gatatactgt
     1681 gttcaaatga tattaaacaa gatttatatt atttgaaagc agtatattat aatgcgaaca
     1741 atacaattct aaactttgca ttgtcaattt acagttcgat aaactaatta aaaattaaat
     1801 ttatataaag agggaaaaga ggaataggga tgtttataag gaaaaatgtt ataaagttag
     1861 caattcctat aatgatagag cagacgtttg tcatgctttt aggagtattt aatacaatga
     1921 tggcaggtca tattggggaa gaagctgtat ctgcagttgg aatggttgat tcaataaaca
     1981 atatatttat atctgtattt gctgcactat ctgttggagc tactgttgtt gtagctcaac
     2041 aaataggaaa aaaagatagt gaaaaggtga atgaaactat taagcaggct ctagtgtctg
     2101 gagtaatatt atcctttaca ataacaatat tgatgtgggt ttttagaact ggtgtaatta
     2161 atttatttta tggttccgca gaagaattgg taaaatcaaa tgcaaaatta tacatagagt
     2221 ttacattatt tacgtatcca tttattgcga cacaacaaat agcaaatggt attttaagag
     2281 ggtgtggaga tgtaaagaaa cctatgtatg tgactatgtt tatgaatata gtaaatgtaa
     2341 cattgggata tatacttata tatggtattg atttaaatcc tctaggaata aatttgcaaa
     2401 caccatccta tggcataaca ggtgcagcta tatctattgc tatagctaga ataattggat
     2461 gtatagtaat tataagagct ttatttaaag gaaacgaatt tatatctatg ggtaaaatat
     2521 ttccatttaa gattgatgtt gaaactcaaa agtctatatt taatatagga ataccagctg
     2581 gagttgaaca actacttttt aatgcaggta aattgattgt tcaagtattt atagttacta
     2641 tgggaacatc agcaattgca tccaatgcaa taggaatgtc aattgcatcc ataataaatg
     2701 taattgggaa tgctttagct ttgtctgcta caaccttggt gggacagtat ataggacgtg
     2761 gagatattaa aggagctaag agtactttat tatatttaac taaatttgca acaatttgtt
     2821 tatttatagt aggagctata tttataccaa ttgcaccctg gatttcaagt atgtatactc
     2881 aaaatgctag tgtaattgac atctcaacac aattaattcg aagtgattgt ttagctatgg
     2941 taatatggcc tatatccttt gtattatcag cagggctaaa gggagccggt gatactagat
     3001 atacaatgat gactgcagta ataggtatgt ggatttttag aatttttaca ggttacgtat
     3061 tgggggttgt attagaattt ggagtgttag gaatttggat aggtatgtat actgattggc
     3121 ttgttagagg gactttatat tgtttaagat taagaggaac aaagtggcta aaacacaaag
     3181 ttgcatagaa tatatgaaat cacttttaga tggataactc tatttagaag tgatttttta
     3241 tgtagttatt atcataatta taagtatgat atataaggtg attaaattac ataaataaag
     3301 gctataatag tatggatatt ataaattata tattgagcag aattacataa tttggcgata
     3361 aatattagaa atatatatct atagaaaata atatatatgg gtatattatt aaatataaaa
     3421 aatattaggt atttatttaa taataaaggt gggtattgtt atgataaata aaataggaaa
     3481 aataactctc tatgtaaata atcaagagga tgcaaagtta ttttggacag aaaaattgaa
     3541 ctttgtagta acttatgaaa aggaaatggg tccaaatatg aaatgggtgg aagtagcacc
     3601 aagtaaaaat gaatttacaa catttgtgtt gtatgataaa aatctaatga tgaagcaaaa
     3661 caaagagata aatgtatctc atccatcaat aattttaagt acaagagata ttaatggaac
     3721 ttatgaacaa atggcaaata acgaggttga ggttggggaa ttaatggaga tgccatatgg
     3781 aaggatgttt tcttttaaag accaagatgg aaatgattat ttggtcagag aggataaata
     3841 attttacaaa gtattaaatt tactcaatat ctgatataat tagagacaat aattattata
     3901 aactagtcta ggattagaat tatataatta aaaattaagt gtaaattttg agtagaggtg
     3961 ataaaatgtt taagaaaatg gcagtactaa aagatatagc aactaaaata ggtcgtaaaa
     4021 aagcgtatga actattagaa atggttgaag gtaatgatgc ctttgtagct gaggtaaaga
     4081 taaaaaagaa tggaatagaa tctaaaaaag aagaaattat gttaaaagat aatcaaaaaa
     4141 taatattaga gtatatagaa ggttaaattt taaatacata taagttgata tactaaattt
     4201 ctgagatgag tttacataaa actcatcttt tttacttttc aataactttg ttttattatt
     4261 taatatacat tttatctcct tagagttgct gatatataaa aattactgat ataataaaag
     4321 aatggatatt aatgtaatat tttatagaaa agaggggtat tatgcttgaa ataaaaaatc
     4381 tttcctttaa tgttgagtct aacaatgagg aactaggaat aataaatgat gttagccttt
     4441 catttgagag gggaaaactt attgttatca caggtccaaa tggtggagga aaatctacaa
     4501 ttgctaagct tattatggga attgaaaaag ctacgagtgg tcagataatt ttagatggag
     4561 aagatataac aaatcttagt attacagaaa gagcaaaaaa aggaatagga tacgcatttc
     4621 aacaaccacc aagaataaaa ggtatgacag ttgaaaattt acttacactt gctcatggaa
     4681 aaccattgtc tactgatgta tgttgccaat accttacaga tgttggattg tgttcaaaag
     4741 actatttaaa tcgtgaagtt gataactctc tttctggagg ggaaatgaaa agaattgaga
     4801 tagcaactct ttttgctaga gatttaaagg tttctatatt tgatgaacct gaagcaggga
     4861 tagacctttg gagctttgga aaactcaatg aaagttttaa gaaaatacat gaagagtcta
     4921 atcagactat aatcataatt tctcaccaag aaagaatatt agagttagct gatgaaataa
     4981 ttgttctaca agatggttca gtaaaaagcc atggaacaaa agaggctata ttacctgaaa
     5041 tcatgtgtca agttaattca agttgtgaat taatgaaaga catgaattaa ttagaaagga
     5101 aggcaaatta aatgaacaaa atagatttaa atcttttaga aaaagttgct gaacttcgtg
     5161 gaactccatt aggagcatac aatatacgta aaaatgggca aggagtagag agacattcaa
     5221 ctgaaaatgt aactataatt cctaaaactg ataaacctgg aatagatatt atagtaaagc
     5281 caaatacaaa aggagaaaat gtacatatac cagtaatagt aacagaggct ggtgtacagg
     5341 atatggtata taatgatttt gaaattggag aaaatgcaga tgttgttata attgctgggt
     5401 gtggtataca taactgtggt tgtgatgata gtgaacataa tggtatacat agatttttct
     5461 tacgtaaagg agctcgacta aaatatgtag aaaaacatta tggtgaaggt gatggagaag
     5521 gaaagagaat tttaaatcca gttactattg tcaatatgga tgaggattca tattgtgaaa
     5581 tggaaacaat tcaaattaag ggtgtagatt caactaaaag agaaatggat gcaaacctag
     5641 gtaaaggagc taaaatagtt gtatttgaga aattattaac tcatggtaat caagttgcag
     5701 aatctaatat gaaggttatc ttagagggtg aaaactcttc tgctcaagta gtttctcgta
     5761 cagttgggca agataattct aagcaaaagt ttaatccttg tgttattggg gattcccttt
     5821 gttctgcaca tgttcaatgt gattcaataa ttatgggaaa ttctcaaatt agtgctatac
     5881 ctgaaattgc tgcaaatcat ccagatgctc ttttagtaca tgaagcagct attggaaaga
     5941 ttgcaggaga ccaaatacta aagatgatga cattaggtct tactgaagaa gaagccgagc
     6001 aacaaatact taattgcttc ttaaagtaaa ttaaagattt tatttaattt aatataatta
     6061 atatagtata atatagtgaa taagtctaaa atatttttat ttttaggctt attttcttta
     6121 tgtaagaaaa gctattagag aaattatatt gtaattagga tgtggaataa atgaaaaatt
     6181 tacttataag aaatgggaca ataatatctc caacagatgg tatcaaattt gaaaaaagag
     6241 atatcttggt tttacatggg aaaatatatg ctattggatt gaaccttaaa gagaaaataa
     6301 gaaaagaaaa attacttaca aataaagagt tcgagataat aaatgccgat aatatgtata
     6361 tttccccagg atttatagat gttcacaccc attgttataa gagaaaatta cctacaggta
     6421 tggattctga ttcaattgga attgaaaaag gttctacagt tatatttgat gcaggtacag
     6481 ctggtccctc aaactactat gattttaaaa aaaagtatat ggatgagtgt aaaacagaag
     6541 tatattcttt tttaaatgtt acaaatgagg gactaaatat attgaatgaa tcaacttatt
     6601 tagatgttat aaattttgac aaggttgagt caattgtaga aaaaaatata gaaaagataa
     6661 agggtataaa agtaaaagcg agtaaatttc aatctaatga aagtggtata gatataatta
     6721 agaaatcaaa gcaagttgct aaaaaattaa atttaccctt agtggtatat ataggagagt
     6781 atccatcata tatagatgaa gtaatgaata ttttgggaaa aggagatgtg gttacaccgg
     6841 tatatggaaa tagtacaaat ggaatcctta aagaatctgg cacattaaga aaatcagtaa
     6901 taaatgcaaa gaatagagga gtattgtttg atgtatgtca tggagtggac caatttgatt
     6961 ttaaagtttt taaaaaagca ataaagcaag gtttagagcc tgacctaata agtactgata
     7021 tgcatataaa aaacatgaaa gacgtaaact attctctttt aaatgttata aataaaatta
     7081 tggaacttgg gatatcccta gaaaagtgta tagaaaaagt aactgaatac ccaacaaaga
     7141 ttttcgattt acatggaaat aaaggcagaa taaagattgg tggagaaggt gattttacaa
     7201 tatttacaat ggaggagtgt aatgatatta taaaagacta caataataat gaacttgttt
     7261 taaataaaaa attaagattg caatatactg taaaatcatg gatgaaaagc agtgaagttt
     7321 atagacatgg atacgaaaat actataataa actaaacaat agtgataaaa taataaatat
     7381 atgtttaaat tggtatatat tggataaata tctaaggaaa caacaattaa aaaataataa
     7441 ttttagggga ggtattttag tatgaaagca tttgttgata aggatgtatg tgtagcttgt
     7501 ggagcatgta taggaacatg tccagacgtt tttagtatga gcgatgaagg gcattcagta
     7561 gcaattgaag gagaaatacc agcagattta caagatttat gtaaagaagc cgaagaagga
     7621 tgtccagttt cagctataac agttaaatag agctgtttaa atttattttt aaaataaata
     7681 tataagattt aattggtact taagctatag tattagaaat ttctattact atagcttttt
     7741 aaaatagata aacttaatat ataagatatt actttttaca aatatggagg actgaaatat
     7801 gaaaaaaaat tatgaaatag tattattcga tttagatgga acacttacag accctaaaga
     7861 aggaataaca aagtctattc agtattcttt aaatagcttt ggcataaaag aagatttgga
     7921 aaacttagac caatttatag gaccaccatt acatgatact ttcaaggagt attataaatt
     7981 tgaagataaa aaagctaaag aagctgttga aaagtataga gaatattttg ctgataaagg
     8041 gatatttgaa aataaaattt atgagaatat gaaagagata ttagaaatgc tctataaaaa
     8101 tggtaaaata ttattagtag caacttctaa gccaacagtt tttgcagaaa ctatacttag
     8161 atattttgat atagatagat attttaaata tatagcaggt agcaatcttg atggtacaag
     8221 agtgaataaa aatgaagtta tacaatatgt actagaccta tgtaatgtaa aagataaaga
     8281 taaagttatt atggttggag atagaaagta tgacataatt ggagctaaaa aaataggtat
     8341 agattctatt ggagtattgt atgggtatgg ttcttttgaa gagattagtg aaagtgaacc
     8401 tacttatatt gtcgaaaatg tagagtctat aaaagatatt ttgttataaa tatcttttat
     8461 agacaaaaat attacaaaat ggtgtcgcat atttaaaaat atgatattat agttaaggaa
     8521 gtcatttaaa aaagaggagg atgcacttaa aatgaaatct attttaatta tgggtggaag
     8581 cgacttcata ggttctgcac ttgctaagcg cttgattaaa tgtgggtatc aaattgatat
     8641 acttacaaat ggtaaaaagg aaatagatta taatggtttt aaagaacatc taatttgtga
     8701 tagaaaagtt cgaaaagata tggaaaacat tatcacagga agaaaatatg attacatata
     8761 tgatatgaca gcatatacaa aagaagatgt aagtaatctg attgatttta taagcatgga
     8821 taacttaaaa aagtatatag tattgtcagc aggagcagta tataaagata gtggaagaaa
     8881 tataaaagaa gaaaatgaga aaggtgaaaa tgaaaactgg ggaaaatatg ggttaaataa
     8941 aaaagaggct gaagatttta ttataaatag ccctatacca tatataatta ttagaccaac
     9001 ttacatatat ggtgaaaata ataatttata tagagagtat tatttctttg aaaaaataga
     9061 aaaaaatgaa aagataccag taccaaaagg aaaacaagtt agtaatcaat ttatatatat
     9121 tggggatttg gtaaaggtat tggaaagcat aatgaaaaat cctcatgtaa gagaggccta
     9181 taatgtgaca aatccacagt taatttcttg ggatgacctt atatatacat gtggagaaat
     9241 tattggcaaa gaacctataa taaaatatgt agatatggaa aaagtagagt ttagagaaag
     9301 aacgtatttc ccatttagaa atatagactt taatcttgat ataaataagc ttattgaaca
     9361 tgggctttat ataccaaacg tgttactaaa agaaggtctt acagcaacat ataaatggtt
     9421 ttcagcaaat aagccaaaaa tgcatgacag aaaaatgaac aaagttgagt ctgtaatgca
     9481 aatagtttaa aaaaataaat aagtaggtag atattttgaa ctcaaaaatt aagtattttt
     9541 taaaattttt tatcttaata tttattttga tttttatata ctcagttgcg atagagccga
     9601 gtctattaat tgtgaaaaaa tataatataa gtgaaaatgg agcattgata aaaaatgatt
     9661 caattgagag taaatctgaa agtgtaaaag tagttcaaat ttcagatact caaataggta
     9721 gtttttattc aactaagaat ctcaaaaaag tagctaataa gattaacact ttgaatccag
     9781 atataatagt atttactggt gatttaattg attactccaa taaaaatcca agtgtagatg
     9841 aaattacaac tattttatca agtatgaatg ctaggttggg aaaattttca gtatttggca
     9901 atcatgatta tatgtataaa cttcctaggt attataggca gattatgaaa aattccaact
     9961 tcaatctttt agtaaatgaa aataaaaaaa taaaattaaa agatgataaa tatattaata
    10021 tattaggtgt tgatgaaata ttaaatggaa atcctaatat aaaatatctg gagagtcaaa
    10081 tagataataa aagttttaat ttattgttag cacatgaacc agatttagta gatatgctta
    10141 gtaaagatac aatgaattta gttctatctg ggcatagtca tggtggtcaa ataagattac
    10201 ctattaaggg agcattagtt actcctccat atggaaggaa atatactaaa gggttttatg
    10261 acataaatgg aaatcattta tatgtgagtt cgggtcttgg aagtacaaag ttaccattta
    10321 gattttttaa tataccagag ataatagaat ttaatattat aatctaacat aaaatattta
    10381 gaaaatataa ttttgctttt tataatatgt agatgtatat actgtaaaaa tggattaaac
    10441 cagtagattg aatgaattaa tagattaaat taatatataa aatatttaag ttataattaa
    10501 tgtattagac aaaataaata ctattaagaa ctatt
//
LOCUS       DAMJZE010000142        10404 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJZE010000142 DAMJZE010000000
VERSION     DAMJZE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.145"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            158..1480
                     /locus_tag="PC541_002921"
     CDS             158..1480
                     /locus_tag="PC541_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2840365.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            1777..3138
                     /locus_tag="PC541_002922"
     CDS             1777..3138
                     /locus_tag="PC541_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2840366.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            3211..4605
                     /locus_tag="PC541_002923"
     CDS             3211..4605
                     /locus_tag="PC541_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2840367.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            4667..5137
                     /locus_tag="PC541_002924"
     CDS             4667..5137
                     /locus_tag="PC541_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2840368.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            5140..7356
                     /locus_tag="PC541_002925"
     CDS             5140..7356
                     /locus_tag="PC541_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2840369.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            7439..8110
                     /locus_tag="PC541_002926"
     CDS             7439..8110
                     /locus_tag="PC541_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2840370.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            8473..9687
                     /gene="dpaL"
                     /locus_tag="PC541_002927"
     CDS             8473..9687
                     /gene="dpaL"
                     /locus_tag="PC541_002927"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2840371.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            9814..>10404
                     /locus_tag="PC541_002928"
     CDS             9814..>10404
                     /locus_tag="PC541_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2840372.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         3883 a         1218 c         2148 g         3155 t
ORIGIN      
        1 taaaacaatt atgttaaaaa attggcatta taattgcttc atattaagtt gagatgacaa
       61 aataaaaaaa gaaaggatgt tgtgtgagat aataataact gtcattaaaa aatagttggg
      121 agtgataacc ttgagtaaat atcaaaagaa tgtttctatg tttgatattg atggaaggcc
      181 accaatttta aagtgtttgc cactatcaat gcaacatata ctagcaatga tagttggaac
      241 tgttacagta cctatagtcg taggaggagc tgtgggtgtt agccctacac aaataacact
      301 tttagtgcaa tatgcattaa taatagctgg tatatcaaca cttatacaac tatatccaat
      361 aggaaatatt ggttcaagat tgcctataat ttttggagtt ggttttactt atgtaccaac
      421 acttgtagca gtcggaggaa attatggatt agcaagcatt ttgggtgcac aattaatagg
      481 gggagtggct acagtattaa tagggatatt cataaaaaag ataagaaaat tttttccgaa
      541 tatagttgca ggaacagtgg tttttacaat tggattatct ttgtatccta tagcaataaa
      601 ttatatggca ggtggtgtaa atagtcctac ttatgcctct atgcaaaact gggcagtagc
      661 ggctataaca ttagtggtag ttataggtct tagtcaattt gcaaaaggat atgcaaaatt
      721 ggcagcaata tttttgggaa ttatagttgg atattgtgta tctttagttc tgggtataat
      781 aaattttgac cctattagag aggcaacttg gtttgcgata cctaaaccgc ttgaatttgg
      841 gatggaattt gatatgcctg taataatctc agtagtaatt gtaagtataa tcaatgctgt
      901 tcaagcaatt ggagatttat cagctactac aatgggaggc atggatagga atatcacaga
      961 taaggaatta tcaagtggag taataggaag tggaatttgt actatgatag gagctttgtt
     1021 tggaggttta ccaccatcat catatagcca aaacgtgggg atggtagcta tgaacaaagt
     1081 aataagtaga tttgtgcttg gaatagctgg tatatttatg ttattgtcgg ggtttgtgcc
     1141 taaatttggt gcactaatga ctactatacc atattctgtt ttaggaggag ctacaatatc
     1201 tatatttagt atgataacaa tgacaggtat aaagttaatt attacagaag agttgtcatc
     1261 aagaaatatt accattgttg gtttggcaat tgccttaggc atgggaataa catcagtacc
     1321 agcagcccaa gaggcatttc cttcatttgt aaagttagta tttggagaat ctccaatagt
     1381 aattgcaaca ttggttgcat tcgttttaaa tctaataata ccaaataaaa gtctagagga
     1441 tgaggctaaa gaaagagctg ttatggaaga gactaattaa gatataataa tttaaaagaa
     1501 ttgtttaaat tatttagtat gagattgtta gattattttt gatagctatt cattttaata
     1561 aaaaatttaa caaataaata tataccaggc aattaatgtt catttaagtt gaaaatcggg
     1621 gagggaatat ttagaaattt gttaattttt tataaaaata gctcagttta gctgtgttta
     1681 tcggttgagc aataaattca tggtcataaa aatatttata gaaaataatt ataaaaatat
     1741 ttagtcaaga tatttatgta tgggaggatt tattttatga aaacacgtaa tacttcaaaa
     1801 tatgatgtcg atgggatacc accactaaag gaatcaatac ctttggcctt acaacatctg
     1861 ttagcaatga tagtaggaaa tatggtacct gcgatattaa tagcaaacgt ggtaggactt
     1921 aaccaggggc aagcaacaat gttgattcag ggttctatgt tagcagcagg tttagcaacg
     1981 tttttacagt tatatccaat acctttattt aaaggattta agttaggttc aagattacct
     2041 gtaatgatgg gaatgagtta tgtattttta ggagcttgtc tttcagtagc agctaaagat
     2101 ggtttagcag ctctatttgg agcacagatt gctgctgggg taatcgtatt ctttgtagga
     2161 tttggagtta agaaaattag acatatattc actccaatag tttctggaac tataatagca
     2221 tgtatgggac ttggactatt cgcaacagct ataaagaatt tagctggagg agaaggcaca
     2281 caaacatttg gaagcccaat aaacttcaca gtaggagtta tagttgcttt tgtaataata
     2341 atgataaata aatacggtaa aggtttagta aaaaattctt caatattaat aggaatatta
     2401 gtaggttatg ctatatcttt agttttagga ttggttgatt tctcagccgt tcaaggagca
     2461 gctatagtat ctttaccaac accagcagct tttggattag aatttagacc agagttaata
     2521 gtaatgttta ctattattta cataataggt atagctgata tgatgggtgc ctgtacaata
     2581 gctacagtag gagcaatgga tagagaggta actgacgaag aattagcatc agttgtttta
     2641 ggaaattcta taacttctat aatttcatca ctatttgcag ctcttccaac tggagtattt
     2701 agccaaaata cagtaattgt atctatgaac aaagttacaa gtagatttgt aatagcacta
     2761 ggggcacttg tattattact agcagggata tctccagcat tgggtgcaat aatgacaact
     2821 ataccatctt gtgttgttgg tggagcaact ttagtagttt tctcatcaat agcaatgtct
     2881 ggtttctcta ttatgagtat ggatggattc actgaagaaa acaatcttat agcaggtgta
     2941 tctatagcaa caagtatggg tcttacaact gctccacaag ttttagacca attcccagaa
     3001 acaattggaa ctgtccttgg agggtcaagt atagtttctg gagcaataat agctatatta
     3061 cttcaaacat tatttaaatt aaagtcacgt aaaagtgctg aaaacgttac ttcaaatctt
     3121 gaggaaaata ttggataaat gtattagata taatatgaga aaaccatatg gtaaaatgtt
     3181 gtaatgaaag tattcaaagg gggatttaag atgattgata tactaataaa aaatgcaata
     3241 attgttacag ttaataaaga cagagatgtt atatttgatg gagcaatagc tattcaaggt
     3301 gacagaatct tggacatagg aaatacagtt gatattgagc ctaagtatct tgatgctaaa
     3361 aaagttatag atgctaatgg aaaagtaata ttccctggct ttataaatac tcataatcac
     3421 ctgttccaag tgttattaaa aggtttaggt gatgatatgg cacttcatga gtggttaaat
     3481 acaatgatgt ttccatcagc aaaattctta acagaacaag atacttatga tgctgcaatg
     3541 cttggatgta tggaaggttt aaaaagtggt ataactacaa tggttgatta tatgcatact
     3601 cacaatagac caggacttac agatggaata gtaaaggcat ataaagattt aggtataaga
     3661 ggggttgtag gtagaggttg tatagattta ggtattcaca aagagcttat agaagatgtg
     3721 gaaacggttg aaaaagattt gagaagggta ttcgaaaaat atcataattc agaaaatgga
     3781 agaataaaaa tctgtgtagc tccttcttca atgtgggcta tatctgaaga aatgggtaag
     3841 atgctttgga atatagttaa agaatatgat tcatatttta cagttcatat atctgaaaca
     3901 gaatttgcaa gaacagcaac aaaggatata catggagaaa tagatataaa attacttgaa
     3961 aaatggggta tagtaggtcc agaagttgta gcagtacatt gtgtatgtat aactgatgaa
     4021 gacatggaaa tgcttaaaaa gtatgacata aaagtttctc ataatgtagc aagtaacatg
     4081 tatcttgctt ctggtgttgc acctgtgcca gaaatgttaa aaaaaggtat aaatgtgagt
     4141 ttaggtcttg acggtgcagc aagtaataac gcacaagata tggtagagct tatgaaatta
     4201 acagctttac aacacaaagt aaataattgt gaccctctgg caatttctgc agagaaagtt
     4261 cttgaaatgg ctacaattga gggagcaaga actcttagaa tggaagatga aataggttct
     4321 ttggagattg ggaaaaaagc tgacctagta atatttaact caatgctttc tccaaaatca
     4381 atacctcttc acaatcctgt atcaactttg gtatactcag ctagtatgca caatgtagag
     4441 agtgttttag ttgatggaaa tgtaatactt gaaaatggaa aagtaactac aattgaaagt
     4501 gaagaacaag tgttgcttaa tgcacaacat gcagcagaag aattatgtaa gagagctaat
     4561 gtaactaata gaatggaagg acataagtgg aatagcttat actaatatta aaataagatt
     4621 gtaataatat tctattacaa cattagttta tctgtaggag gagaaaatgg aaaaaataaa
     4681 attaaatatt aatgattaca tgtatgaaat agaagttgaa aagaatgaaa ctcttttgca
     4741 tgtacttcgt gaaaaattaa atctaactgg agcgaaacaa ggttgtaata gtggcagttg
     4801 tggagcatgt aaagttttga tagatggagt tgacatgaaa tcttgtaaac ttcttgcaaa
     4861 aaactgcgaa ggtaaagtta tatacactat agaaagtttt gctaatggag aaaatcttca
     4921 tccaatacaa gaagctttta tagaagtcgg agctgtacaa tgtggatatt gtactcctgg
     4981 tatgattata acaacacaag ctcttttaaa tagaaatgca tctcctaatg aagatgaaat
     5041 tagagaagct tttaaagaaa atctctgtag atgcacagga tatgtaaaga ttatagaagc
     5101 tgtaaaatta tctgcagcta gattaggggg tagataatta tggaaaaggt aataggaaaa
     5161 tcataccctc taaaagatgc agctttaaaa gttactggtc aaatgaaata cgtggctgat
     5221 atgaaaccac aaaatgtact ttacgctaag atgcttttaa gccctgtagc acatgcaaaa
     5281 ataaaaagta tagatacaag tgaagctgag aaagtagtag gtgttagagc tatagctact
     5341 tatctcaata catcacaaag aaaatacaat ggttcattaa gattttatga gcacaaaata
     5401 cctgaaaatg aagttatatt ttcagataca gttagatttg ttggtgaccg tgttgcagca
     5461 gtagctgcag agactccaga aatagcagag aaagctgtaa aacttataaa agttgaatat
     5521 gaagaattgc cagctgtatt tactatacaa gatgcaataa aagaagatgc tatctgcata
     5581 catggagatt caaatatagt ttctcaaaac aacataaatg ggggagatgt agaaaaaggc
     5641 ttcaaggaat gtgattatat atttgaagat gaatactcta ctccagcaat acatcatagt
     5701 gcaatagaga ctcatagtgt aattgcacac tatgattata atgataaatt aactgtacat
     5761 acaccttgcc aaaatacatt tggatttaga ataatactat ctcaaatttt tgggatgtca
     5821 ttaaataaag taagagttat aagacctgct attggaggct catttggtgg aaaattagaa
     5881 cttacaattg aaccagtagc agccgcatta tcaaaaatga caagaagacc agttaaaatg
     5941 gttcttacaa gaaaggaatc tatgatatca acaagaactc gtcatgggtc ttttgtaaaa
     6001 ttaaaaactg gtgtaaataa agatggtgaa gtaatagcac aagacataaa aatatataca
     6061 aatacaggag catatgcatc aagtgcttta aatgttatag gagcacttag tcataaagta
     6121 tttaaagttt ataaaatacc aaatataaag tttactggga tgcctgtata tacaaataca
     6181 ccaatagcag gagctatgag aggctatggg tcacctcaaa tatttatggc tcaacaagct
     6241 caatttgcga aaatagcaaa ggaaataggt atggatttag tcgattttca aaataaaaat
     6301 gcagtagagc cagatgatgt cgatataatc ttccacggtt ctcttggaaa tcctagagta
     6361 ttggattgta tagagcaagg taagaagatg tttaagtggg atgaaaagaa aaaacttcca
     6421 aaagaagaag gaaaatattt aagaggtata ggtatggcaa taggtgcaca tggtaatgga
     6481 gtttttggtg cacatagaga tgttgttaca ttgactctta aattaaatga agatggtacg
     6541 ttgacactac taacaggagc tcatgatatg ggtaatggtg ttgtaactat gcaaacaatg
     6601 atggtagctg aagttttagg aataacacct gataaagttg atacatttga aactgatacg
     6661 gatgcatgta cattcaacct tggtgactat gcaagtagag gggtatttgt agaaggtgga
     6721 ggagctaaaa agacagcaga gaaattgaaa aatcttatat tagaagaagc agaaaaatta
     6781 cttgaaacat caaaagaaga tttatatcta gacaatggat gtgtaattag taaacttgat
     6841 gaaaatgtaa aagcatcact ttctgatgtc gcagtatata gtcaaagtaa atcattaaga
     6901 gagttaactg ttgttgaaga ttattcttca ccagctggac gtacttctta tggtgtacat
     6961 tttgctgaag ttttagtaga taaagaaact aaggatataa aagtagttga ttttgtagct
     7021 gtacatgatg ttggtagagt tataaatcct ctaaatttag aaggacaatt agaaggtggg
     7081 attcatatgg gtctaggata cgcattgagt gaagaattgg catttgatga acaaggtaga
     7141 cctaaagcta ctgattttaa aacatataaa ttatttagaa ctgtagatat gccaaaatgt
     7201 caagttgcat ttgtagatgg ttatgaaaaa gctgggcctt ttggtggtaa gagtattgga
     7261 gaatgtgccg ttgtaccagt tgcgccagct gttgcaaatg cagtatataa tgcaactgat
     7321 gtagagatac attcattacc aataaaatta aaatagtaga aattaaagca gaaataacaa
     7381 tttattgtat ttgtaaatag ataataaaaa tagatttatt tggtgaggag atattattat
     7441 ggcaaagata ttaggtattt gtggaagtcc acgtaaagga gcaacagaat atgcagtgtt
     7501 agaagctttg aaagaagcag aaactatacc tggtatagaa actgagtatt ggtcagttag
     7561 agggaaaaaa ataagtcctt gtgtacattg tgatgcatgt ataagaaaag aaactatgtg
     7621 tattataaaa gatgatatcc aagaattaga gcaaaaaata ttggaggcag atggaattat
     7681 agtaggttct ccagtttatg atatgaatat aactgctcaa cttacagcag tatttaatag
     7741 acttcgtcca atgtatttag ttcatccagg aaaacttcaa aataaggttg gtgcagctat
     7801 aactactggt ggaactagat atggtggcca agaattgaca aaacttccta taataaattt
     7861 cttcctaatg catgaaatgt tagtgtctgg aggtttaggt ggatgctata taggtggtac
     7921 aatatggagt aaagatggaa aagcaaaagg tgcccaagag gatgagacag gtatggacac
     7981 agtaaaaaga cttggaaaag gtgcagcaga ggcagttatg gtttctaagt ttggtcttga
     8041 aaaatggaat gtcgtaaaag aagaattaga tgttaaaaaa gatgaaaaat cacctcttag
     8101 agaccactaa agatttatta tattattgaa tatatacaaa tagtgatgta atagattatt
     8161 gcatcactat tttatgtttt aatttagatt ttataaattt tatattaaaa aatatagaaa
     8221 caaagtgttt tgagatttat ttttaaattt taaaaatgag aattaaaaag tgagaagata
     8281 gaaaaggttt tttagttatt aaattctaca tacattgtat gaactaaaga tagaaaagaa
     8341 aaaataataa aagcattgca atttctatac taaagagtca tggaaaaata tctagtatat
     8401 atttagtatt ggcatgttaa ttgcttatat attaaagtat aaaaatttaa caataatttg
     8461 gaggtatttt agatgttaaa agaaattaaa tggaaagtaa ataatttgcc aaagggcgat
     8521 aaggaaaatt gtatcaaatt tttaaatgaa gaagaaataa ctaaggttag aaattttcac
     8581 aaaagctttc ctcaatataa agaaactcca ttagcaaacc tagaaggttt agcaaaaaaa
     8641 ttaggtgttg caggagtata tgtaaaagat gaatcttata gatttggatt aaatgcattt
     8701 aaagtattag gtggttctta ttctatgggt agatacttag ctcaaagatt agatacagat
     8761 atatctgagt tagggtatga caaattgact tcgaaagaaa taaaagaaaa attaggagaa
     8821 ataactttct ttacagctac agatggaaat cacggtagag gagttgcttg gacagctaat
     8881 aaattaggtc aaaaatctgt tgtattaatg ccaaaaggtt cttctgaatt tagattaaat
     8941 aaaattaaag gagaaggtgc agatgctagt ataacagact taaattatga tgatgcagta
     9001 agattagcta atgattatgc agaagctgat gaccatggag taatggttca agatacagca
     9061 tgggacggat atgaagaaat accagcatgg ataatgcaag gatatggaac tatggctcaa
     9121 gaagctatag agcaattaaa agaatatgga gtagatagac caacacacgt atttgttcaa
     9181 gcaggagttg gttcacttgc tggtgcagtt caagggtatg ttgcttcaat atatgatgaa
     9241 tgcccaataa cagtagtagt agaggctgat gaagctgatt gttactataa atcagcagaa
     9301 gctggagatg gaaaaccaag atttgttgga ggagatatgc caactataat ggcagggctt
     9361 gcttgtggag agccaaatac tataggattt gaagtattaa aaaatcatgc agcagcattt
     9421 gtttcagcac cagactgggt ttctgctaaa ggtatgagaa cattaggtaa tcctttaaat
     9481 ggtgatgaaa aggtaatttc tggagaatct ggagcagtta caactggtct tttagtagca
     9541 gctatggaaa gagaagattt agcagattta agaaaagatt taaaattaga tgaaaattca
     9601 agaatacttt taataagcac agaaggagat actgaccctg acaaatatag aagtatagtt
     9661 tgggatggag aatatccaag tatataagga attactaaaa tgcaattatt agatattggt
     9721 ttacatattt agaagatata taagttctaa aatttagaaa atatataagt atataaaata
     9781 catacattat aaaaaatcta gggggataat gaaatgttaa acgaacaaag aaaacaagaa
     9841 gtaactgaaa tatgccaaaa attaattcaa aatcctagtt cttctggaaa tgaagaaggt
     9901 gttgtaaaag caatagaaga gtcttttgaa aaattaggtt ttgatagttg gtcaagagat
     9961 agatatggaa atatagtagg atgtataaaa ggtaacaagc caggtaagaa aatattattt
    10021 gatggacata tagatacagt tccagttcca gatgcaagta aatggagtgt tcctcctttt
    10081 gaaggaaaaa tagttgatgg aaagatatat ggtagaggaa cttctgacat gaaaggtcaa
    10141 tatactgcaa tgatgtctgc tgtagcgtat tttgctaaag acactaatag agattttgct
    10201 ggagaactat atgtagcagg tgttgttcat gaagaaatat ttgaaggtgt ttcggctaga
    10261 gagataagta aggctgttca acctgactat gttgtaatag gtgagtcttc agagttaaac
    10321 ttaaagattg gtcagagagg tagaggagaa atagtagtag aaacttttgg taaaccagct
    10381 cactctgcta atccagaaaa aggt
//
LOCUS       DAMJZE010000143        10383 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJZE010000143 DAMJZE010000000
VERSION     DAMJZE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.146"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            105..2606
                     /locus_tag="PC541_002929"
     CDS             105..2606
                     /locus_tag="PC541_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2840373.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2766..3434
                     /locus_tag="PC541_002930"
     CDS             2766..3434
                     /locus_tag="PC541_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840374.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3418..4695
                     /locus_tag="PC541_002931"
     CDS             3418..4695
                     /locus_tag="PC541_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2840375.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5096..5317
                     /locus_tag="PC541_002932"
     CDS             5096..5317
                     /locus_tag="PC541_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840376.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5606..6370)
                     /locus_tag="PC541_002933"
     CDS             complement(5606..6370)
                     /locus_tag="PC541_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2840377.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6644..7318
                     /gene="fsa"
                     /locus_tag="PC541_002934"
     CDS             6644..7318
                     /gene="fsa"
                     /locus_tag="PC541_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2840378.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7440..8834)
                     /locus_tag="PC541_002935"
     CDS             complement(7440..8834)
                     /locus_tag="PC541_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2840379.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(8912..10258)
                     /locus_tag="PC541_002936"
     CDS             complement(8912..10258)
                     /locus_tag="PC541_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2840380.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         4226 a         1232 c         1606 g         3319 t
ORIGIN      
        1 aataaaaata tagatttcta aataagtaat ttatcaatat tacatatcaa aataaaacgt
       61 tacaaattag ctgaaaatat attgaaagaa aggaagattt tattatgagt tcaaataaag
      121 tggtagaaag ttataaaatt acaggtatga catgtgcagc ttgtgcaaag gcagtagaga
      181 gagtaactaa gaagatggat ggagtatatg accaaagtgt aaatatagct actgaaaaac
      241 ttaaaataga atatgataat tctaaggtta attttgatga tataaagcag gttgtagaaa
      301 aagcaggata tggaattata aaggaagaaa gtaataaaaa aattgatatg aaaattgatg
      361 gaatgacatg tgcagcttgt gcaaaagctg tagagagagt ggtaaaaaaa ttggatggag
      421 tagaaagtat tagtgtgaat attgcaacag ataaagctaa tatagactat gacccatcaa
      481 aagtaaaatt atctcaaata aaagcagcta ttgaaaaagc tggatataaa ccaatagaag
      541 aggtaaaaaa taaggttgat gtggatgaag ataaattgag aaaagaaaga gaaatgaagt
      601 ctttatttgt taaatttata gttgcaatag tatttgcagt acctctgttt tatatagcaa
      661 tgggacctat gataataaag cctatagggc catggccttt gccagaaatt ataaatccta
      721 tgacaaatac ttttaattat gcattaattc agttaatttt agtaatacca gtaatgatag
      781 ctggttataa attttatata aatgggttta aatcactttt ttctttaagc cctaatatgg
      841 attctttagt tgcaatagga acattagccg catttttata tagtctatat acgacactac
      901 aaatagccaa tggacaaatt caaggtatgc accatcatca gttgtattat gaaagtgcag
      961 gaataataat agcacttatc ttacttggta aatatttaga atcaaaatca aaaggaaaaa
     1021 catcagaagc aattaaaaag cttatggggc tacaacctaa aactgcaata gtgttagttg
     1081 atggtaaaga agttgaaact cctatagaag aagtagagat aggagatata ctattagtaa
     1141 aaccaggaac taaaatacct gtggatggag ttgtaataga aggatataca tcagttgatg
     1201 aatcaatgct tacaggtgaa agtataccag tagaaaagaa tgttggaagt aaggtgacag
     1261 gagctagtat taataaaaat ggtgttatta agtttaaggc tgaaaaaatt ggtggagata
     1321 ctgctcttgc ccaaataata aagttagttg aagatgcaca aggtacaaaa gcacctattg
     1381 ctaaacttgc cgatactgta tctggatatt ttgtaccaat agtaattgca atagctgtag
     1441 ttgcatcatt attgtggttt ttaataggag gaaaagatat agtatttgta cttactatat
     1501 ttatatcagt acttgtaatt gcttgtcctt gtgcattagg attagcaaca ccaactgcta
     1561 taatggttgg gactggaaag ggcgcagaaa atggaatctt gataaaaggt ggagaggcat
     1621 tagaatctgc acataaagta aatacagtga tatttgataa aacaggtact ataacagaag
     1681 gaaagccaaa agtaacagat atagtattaa ataataatgt aaaagaagaa tatcttatca
     1741 agattgcttc aagtgcagaa aaaggttcag aacatcctct tggagaagca atagttaagt
     1801 atggagaaga gaagaatata aaatttgaaa aagttgataa ttttaaagcc ataccaggtg
     1861 ctggtataca agttactatt aatgatgaaa gtatattact tggaaataga aagcttatga
     1921 atgacaataa tataaagctt ggggatttag aagaaaaatc aaatatattg gcaagccaag
     1981 gtaaaacacc tatgtatata gctgtagatg gaaatttatc aggtataata gcagttgcag
     2041 atgtagtcaa agaaagtagt aaaaaagcaa tagaaatact tcatgatatg ggaataaaag
     2101 ttgcaatggt aactggagat aatgcgaaaa ctgcaaatgc tatagcaaat caagtcggta
     2161 tagatatggt tttggcagag gttttaccag aagataagtc aaaagaagtt gaaaaactac
     2221 aaaatcaagg aaagtttgtt gcaatggtag gggatggaat aaatgatgcc ccagctcttg
     2281 caaaagcaga tatagggata gcaataggaa gtggaacgga tgtagccatt gaatctgcgg
     2341 atatagtact tatgaagagt gatttaattg atgttccaac agcaattaaa cttagtcatg
     2401 aaactataaa aaatataaaa caaaacctat tttgggcatt tggatataat acaattggta
     2461 tacctgtagc agcaggaata ttatatgtat ttggaggacc tttattaaac ccaatgattg
     2521 cagcagcagc aatgagctta agttcagttt cagtcgtatc aaatgcactt agacttaaaa
     2581 acttcaaggc atataagaga gattagatta ataaattaaa taaaatgtat ccaaaagagc
     2641 tatctttaaa ttaaaaggat taatttacaa attaagatag ctcttttttc atgtaaaatt
     2701 cacaacaata agataaaata taagtaatat acgaaatttt aaataaaata gaaaggattg
     2761 atagaatgaa ttcgtcaata cttgtaattg aagatgattc aaatatacaa gagttaatat
     2821 cagaattttt aagtgcagaa ggttatcaag ttgatacagc aaatgatggg ttggaaggta
     2881 tacaaaagtt taagcaagga agttatgatt tagtaatatt agatataatg atgccaaact
     2941 tagatggtta tggagtttgt aaaatgataa gaaagtcatc aagcgttcca attatatttt
     3001 taactgcttt aaatgatgaa ggagaccaac ttaaagggtt tgacttagaa tgtgatgatt
     3061 atataacaaa accattttca tttaatcttc ttataaaaag agtagaagca attttaaggc
     3121 gaagtaacaa gactataaat gacaaattta tagtttttga aaaattaaaa ttagatttaa
     3181 acacatatat tgcagagata gatggagaac ctatagaact tacactaaaa gagtttaata
     3241 tattaaaagc tttgatagaa aagtatccac aagttataac tagggaaggt cttttggata
     3301 gtatatgggg ttatgactat tatggagata ctaggattgt tgatgcacat ataaaaaata
     3361 taagaaaaaa aatatcatta ccatatatta aaactgtaaa aggaatagga tatacacttg
     3421 agaaggatat ttgataagtg ggaaaagtta agtataaagt ataagttatt ttcaataacg
     3481 acatcattat taatagcatt agctttaata atatatctaa ttttatattt tttattgcca
     3541 tcatattatc atgagtataa aattgagtct cttcaagaaa gtttgaaatc tttagtagat
     3601 agttcaattc atttcgatac atatacttta gaagaaagac tttattatat ggctaaagac
     3661 caaaatttgg ctattttatt aaaagataac caaggtaaaa ttgtatatgg taagaatgaa
     3721 gtagttattt taagatatag taagtacatg attaatagtc ttgaggatga gtatagaact
     3781 tctataccta tatatactaa agatgcaaaa gatggtccat atactttaga actcgttatg
     3841 ccattacaac caattgacga agcaaacgaa gttataagaa aactaatgcc atacatcata
     3901 tcaatagcaa tattgatagc gattattgga gcatatatat attcaattgt aataacaaag
     3961 cctcttataa acataataga gagtgagaga gaacaggaat atagaaggaa ggattttgtt
     4021 gctacaatat cacatgaact aaaaactcca ataaccataa taagtggtca aattgaaggt
     4081 atgatttata gtgtaggtaa gtataaggat agggatactt atctgaaaaa atcgtatgag
     4141 tgtacacaag aattaaaaga tttagtaaat gaaatgatag aggtttcaaa atcagaaata
     4201 cttgaaaaag atttgaaatt ggtatcaata aatataagtg aattattaaa tagacttgta
     4261 aagagacaag tatttttaat agaagaaaaa catatgaaaa ctattttaaa aatagaagaa
     4321 aatttggaaa ttaaagcaga ccaagagaga ataactaagg caatcaataa tataataaat
     4381 aatgccataa aatattctcc tgaagaatca gagcttataa ttagactcta tgataaaaat
     4441 aaacgaataa gtaaaaagac ttctaatcaa agagtggttt tagaaattga aaatactggt
     4501 gtaactatcg agaaacgtta tttagaagaa atatttaatc cattttatag aatagaaaaa
     4561 tctcgttcga gaaaaactgg aggaagtgga cttggacttt atatagtcag ccaaattttt
     4621 aaaagtcatg ggtttgatta tagcataaaa aacaaagaaa actctgtagt attcacagta
     4681 gaatttaaaa attaattact tattataaag aatatataac tttcaataat agttcatttt
     4741 aaaatcacaa aaaattcata atgaaactgt attatataaa tataaataag cttaaaacca
     4801 taaaatacac caactataat gatgagattt ccttacatcc tccatataag tcatcattat
     4861 agtaacccaa caattaagtt actatgaatt taaaaaataa caccgagcat ataatctatg
     4921 cttagtgtta tttttatttt gaaataaaaa atatttaatt tattgaaatg tatagattta
     4981 gatagtttaa actatattta agacttaaaa attataattg atattacaaa taaatatatg
     5041 tactttggga aaaataaata taggaagtat tatatttaca gaggaggtag ttttgatgga
     5101 tagattaaaa agagatatga aaaatatgaa agaagattta aaagaaggtg ttaaaaagac
     5161 agcttatgtt atgaaaaata cagctgaaga tttgaaagat gatgcacaag gtgctatggt
     5221 aaacttagaa atgaaaaaag atgagatgat ggaaagatat gaacaaaaga aattttctaa
     5281 agaattagag aagcaaatga agaatgaaat gaaataagct tttatttatg aataacaaat
     5341 tattgtattc atataaaaag tagccagtaa tattaaatag tttatttaag gaaatagact
     5401 ttctaaaaat aactgtatta gatttagaaa gtctatttca aattagtttt ataaatagac
     5461 ataaggataa agtagatgtt atagaactag attttaaaaa atagatacta aggataaaat
     5521 aaatattata gaaatatgtt tttaagaata tttatcaaat aaaaatttat atttataaga
     5581 aatcctataa aacatataat taaacttata aaacatttac attaaattct gataaaatat
     5641 ctactatatc aggagtaggc gttttttctg taattataga actaatatct tctaaatgag
     5701 caaacttgaa atttccatca aatttaaact ttttatcttc cataactata aatacttttt
     5761 tacttgaatt tattatagcc ttcttcgtaa gaccatcttc aatttcaaaa gttgtaagtg
     5821 aagagttgaa tacatctact ccacaacttc ccataaaagt cttatcaaac ttatatttcg
     5881 atataaactc tatagttgga gtaccgacaa aaccgtctaa actgagattt aataaacctc
     5941 cagtggaaat tatatttaaa ttatttggtt ttgaagtaac tacatttagt atatccagca
     6001 tatttgtaac aagtgttact tttttattac tttcagctaa caacttcgca agcaagatgt
     6061 taattgtaga gatatctaaa aatatagttt ctctatcttc aattaaatta aatgcttttt
     6121 ctgctattat tttctttgtt ggaatattaa tttcttttct gtcttttgta tcctgattct
     6181 ctagtgtttc tctagctagt acagctcctc cataaattct tcttattgaa gaaatctttt
     6241 ctatattttt taaatctttt cttatacaat cttctgtaac attaaattta gaactgagct
     6301 ctttgacagt aactctacct tcttttttta ggatatttaa tatagcttgg attctttctt
     6361 ctgcaaacat tttctacctc ttttcgcaat atagtattat agtaaatatt ataagataaa
     6421 atacaatata tcaactattt taacatgaaa tacaaaatta atttaatata aaaagtatgt
     6481 tttttctaac tatatattta gaatttagta aataaaaaaa ttataaaact aagaaggaaa
     6541 agtaaaacaa taataaaaaa taaaaaacaa taagcaaaat gttgaaaata ataccaaatc
     6601 aatatataat aagaataaag aaagattaaa gggggatatt ttaatgcaac taatcttaga
     6661 tacaggtaat gtggaggaga ttaaggagtt atgtacttgt ttaccaatag atggtgtaac
     6721 tacaaatcca agtattgttt caaaagaaaa aaagaatttt aagcaactta taaatgaaat
     6781 tggtgagatt ataggtgagg atatgcctat acatgctcaa gtgttaagta caaagtatga
     6841 agaaatatta gaagaagcat tatatataag tagtttgaga aaaaatatat atgtaaaaat
     6901 acctgtaaca caagatggtt taagagctat aaaagacctt cacagaaaag gtgtaaaaat
     6961 aactgctact gcaatattca ctgctcatca aggtttttta gctgcaaagg ctggagcaaa
     7021 ttatattgca ccttatgtaa ataggcttga taatatatct ggtgatggag tagctatggt
     7081 ttcagaactt ataaaaataa tagatactta taaaatggat acaaaagtac ttgcagccag
     7141 ttttaaaaat gcacaacaag ttatagaatt aatgcaacat ggggtgcata gtgttacagt
     7201 accatatgat atttgtaaat ctatgatgaa tcatccatta actgattgga gtgtaggtaa
     7261 gtttatagag gattgggaga atacttttgg aaaaggaagt aaaacaaata atatttagta
     7321 ttatggtaaa agagtagttt tgtccaattt cacaagaact tatataaaaa gatgaaaata
     7381 tgaattttct ttttaataat ttcgttatta tcttaagtta attcatattt tcatcttttt
     7441 tagatttcta gtcctataaa tttccaccaa gggaatgtta tacatattat tataaagtag
     7501 gttaaaggaa tcaaagttaa accgtatttg aaaataacct tgctattata cagattttca
     7561 ccatgaccaa ccattattgt agcatgatga aatggtaata tgtactgtat gcttactata
     7621 atgtagacaa atagacataa agtaacacta ttttccttta gtatatgtaa acttccaagt
     7681 aacggtatta ctacagatga tgtagtgaca gcacttccaa gacatatatt caagattatt
     7741 gtgagagtta tcaaaaacaa tattgaaata atttctgaag agtttggtat aaactttatc
     7801 aaataatttc ccattatatc tacaactcca gagtacttta acactcctcc aattgaaaat
     7861 gctgctataa aaaaaataag gaccttccaa tttatttctt ttaaagtact aaatccaact
     7921 atttttctta gtaataaaag aagtacacaa attgacatta taattaatgt gtttatgtta
     7981 tgtatgaact gagttaaaaa cataaaaaac attattccta ggcataaaaa tacgtgtttt
     8041 tgctctttgt ttagcaaatt aaattctttt tttaaattag aatatgaaaa atgaagttta
     8101 ttaccctcaa gattttcatt agaaataaat tttattgaat gcaattcttt tttaaagaca
     8161 aagataaata aaaaataagt tataaaacaa gtaattatag ttggaacaga catataaaaa
     8221 gcccattgac cccaatttat attacaattt ccaagttcta gtacaacata atttagaagg
     8281 gtgtctccat ttacaaaaaa cattgatgta aaggtagatg ctgtaaatat actaaataaa
     8341 agtatagatt ttgaatcttc agttatctgt tgttctttta aaaactcctt gtaaaaagca
     8401 gatacaagaa taacccttgg aaatggttgt gggataaaaa atattaaaag tatacctaaa
     8461 atatacgata aaagaatcat ttttttaggg gtatttacag tatttaaaat taacttctca
     8521 gaaataattt ttgcgacacc tgtttttgta actgcattag taattatgta tgcaagaatt
     8581 attatgtaaa aattttctgt aaataaaaac tgtaaaataa cctttattgg tgcaatgtta
     8641 aatataaacg ctagaattac aaaaaacaat gcaacaaaag ttttttcaac tgagtctaca
     8701 gcccatatca agatagttaa aaaagataag catatagaaa attttatgtt gaaactgtaa
     8761 ttaagagaga agtagagaat tatggtagct ataacagtca tataaaattg ttttggctta
     8821 agaataatac ccatttttat attcacctcc ataatttatt atatcataat ggaggtgaat
     8881 acaatgaatg tgaatacatt ttataaaaag attattcatt gattctatga tttacaatag
     8941 ctttagtttt ccaatgtcct ttcttatagt agataactgt aacaataaga cctaaagtcc
     9001 aaccaaaagg aatacccatc caaattccgt ctgttccaaa aaatttagaa agcatataag
     9061 aagctggaag tcttccaagc cataaagata ctattgagct tataagtgca aacatggaat
     9121 caccagcacc tttcattgca cttgaaagta caaaagaagt tccaataaat aagaagaatg
     9181 gtcctattat aaataagtaa gaagagccaa ttttaatgac atctttatca gtattgaata
     9241 gagcaattag attttcccta aatagcaaaa ttaaagcaat aactgtaact gctaaaacta
     9301 aggtcatttt tatagattct cgaatgcctt ttttaactct ttcattttct ccagcaccta
     9361 tgttttgagc aacaaaagta gaaacagcag aaccaagatt cataataggt aagcttataa
     9421 aattttctat cctaagacca gcaccaaaag ctgccatagc agatgtacca taactattaa
     9481 ctaaaaattg aagagccata tttccaattg aaaacaacat ttgttgaact ccacttggaa
     9541 gtcctaatct aagtccatct ttaaatattt tgttatcata tacaattcct ttgattttaa
     9601 atttaagcac ttcatgtttt ttatttaaat aaattatact tattataaat gatactgcct
     9661 gagccattat agtggcaagt gcaacgccct caacacccat tcttagcact acaataaaag
     9721 ttaagtctaa aactatattt agaattgtgg ctattatcag aaaataaagt ggagttactg
     9781 aatctccaag tcctctaagt attgcactta ctgaattata tccagcagaa aataatattc
     9841 cactaaatat tatagtaaga tacaattttg attgagcaaa aacggattct ggtgtattca
     9901 taagcttaag cattgggcca ccaaatacta ttccaagaat acttattaat atagaaccaa
     9961 taaataaaag tatatatgta gtatcaatag tagcttttac cttttttaaa tctcctgagc
    10021 cataaaattg tgacactaaa atagaaaatc caaggctaac acccataagc gctgccacca
    10081 ataaaaacat tatagggtta gctccagaga ctgccgccat tgcttctttt ccgataaatc
    10141 taccaactat tatactgtca gctgtattgt atagttgttg aaataaacta ccaataagca
    10201 taggcatggc aaaaaagaat atggactttc cctcatgacc tgtagttaaa tctttcacct
    10261 tattcctcca aataaaaaaa ttattaattt attatagtat aatcttaaca taaactttat
    10321 atccaaacta taaataaatt gtatcaaaat atcatatatt tttttaaaat gtaaagaata
    10381 taa
//
LOCUS       DAMJZE010000144        10335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJZE010000144 DAMJZE010000000
VERSION     DAMJZE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.147"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            253..1677
                     /locus_tag="PC541_002937"
     CDS             253..1677
                     /locus_tag="PC541_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2840381.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1712..3841
                     /locus_tag="PC541_002938"
     CDS             1712..3841
                     /locus_tag="PC541_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2840382.1"
                     /translation="MKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDVVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3871..4293
                     /locus_tag="PC541_002939"
     CDS             3871..4293
                     /locus_tag="PC541_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2840383.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4312..5463
                     /locus_tag="PC541_002940"
     CDS             4312..5463
                     /locus_tag="PC541_002940"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2840384.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5569..5670
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5853..6425
                     /locus_tag="PC541_002941"
     CDS             5853..6425
                     /locus_tag="PC541_002941"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2840385.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6489..7139
                     /gene="fsa"
                     /locus_tag="PC541_002942"
     CDS             6489..7139
                     /gene="fsa"
                     /locus_tag="PC541_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2840386.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7291..10215
                     /locus_tag="PC541_002943"
     CDS             7291..10215
                     /locus_tag="PC541_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2840387.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4234 a         1009 c         1940 g         3152 t
ORIGIN      
        1 tttaactgaa ttattatttt catttctatt aaatttaaat attttataaa aatagtatta
       61 tttacaaaat catatactcc aaaatactac ataaataaaa ttaaacaaaa actaaaatga
      121 caaatgtcat tatctaaagt gacatagaaa cggtttctta aagttgttac tataaaatat
      181 aaactctata ataaaagtat aacaaaaggg aatcatatcg atatagaata taattcaaga
      241 ggaggtaaaa ttatgagtag ttctaatcaa tcgacaataa agacatctat acagcgagta
      301 gggaaatttt taagtggtat ggtaatgcca aatataggag cttttatagc atggggtctt
      361 ataacagcat tatttatacc aacaggatgg atgcctaatg aaaaattgag tactataggt
      421 gacccaatga taaaatacct tttaccacta ttgatagctt atactggtgg taaagcaata
      481 gcaggacaaa gaggtggcgt aataggtgca gcagcagcta tgggagtaat tgttggtgca
      541 gatataccaa tgtttattgg agcaatgata atgggaccat ttgcaggatg ggtaattaag
      601 aagtttgata aatttgtaga tggaaagata ccaacaggat ttgaaatgtt agtaaataac
      661 ttttcaatag gtataatagg tatgcttctt gcaatactag gattttatgc aattggacca
      721 gcaatagtag cagggacagc tcttatagaa tctggagttc aatttatagt tagcaaaagt
      781 ttattgcctc tagtttcagt atttatagaa cctggaaaag tattattctt aaacaatgca
      841 ataaatcatg gtatattagg gcctataggt atagctgagg caaaagaagc aggtaaatct
      901 ataatgttct tactagaaag taaccctgga ccaggtttag gagttctatt agcatattgg
      961 atgtttagta gaggaagtgt aaaacaatca gcaccaggag ctgtaataat acatttcttt
     1021 ggtggtatcc atgaaattta cttcccatat attttaatga atccagtttt aatattggct
     1081 acaatggcag gtggagcagc aggaatattg actttttcaa tattaggagc tgggcttgta
     1141 gcagcaccat caccaggaag tatatttgca cttatggcac tagctccaaa gggaggatta
     1201 cttccagtat tagcaggagt tgcagtggca acagttgtat cattcttagt ggcagcacca
     1261 tttgttaaga gagcttcagc aaatcaaagt gaagaagaca gtacttcttt agaagaagct
     1321 aaagcaaaaa tgagtgatat gaaatctgca agtaagaact ctgagaaaaa tattgaggaa
     1381 aaacagcttg aagtaaatga gattaagaag attgtatttg catgtgatgc aggaatgggt
     1441 tcgagtgcaa tgggagcttc cagatttaaa aatagaataa aaaacttaga cttaaatata
     1501 gaaattacaa attcatctgt tgataatctt ccagatgata cacaaatagt agtaactcac
     1561 aatacattag ttgaaagagt tgctaagaat aattcaagtg ttgagatagt ttcaataaac
     1621 aattttttaa atgacccaaa tttagataca ttgtttaaaa gattagaatc taaataaaaa
     1681 taaaaagtac cactaattag gagtggataa tttgaaaaaa aagaagatta catctaggca
     1741 aaaaaagata atactaatga tagtagcaaa ttcaaagaaa aacattccaa taactatttc
     1801 tgagattgca gggactttag agttaagttc aagaactgta cttagagata tgtctggaat
     1861 tgaaaaatgg tttgatgaaa atgattttaa ctttgtaaaa aaaccaggag tgggattgat
     1921 tttagaggaa aatatagaaa atcaaaactt tataattgag ttgttagaag aagaaaaaat
     1981 agaaaaagaa tacagcaagg aagaaagaaa tttaattata ttatcaaagc tactagtttc
     2041 taacgagcca gtaaaatcct attattttgc aaaaatatta aaggtatctg aatgggtact
     2101 gaataatgat tttgccttag caagtaaatg gttggaaaga tttgatattg agcttgttag
     2161 aaaaccaggg ctcggtgtct acttaaaagg tcaagaaaaa aactttagag aggcatatgt
     2221 aaatcttata tacgattcat tcaatgaaaa agaaatacta gatatggtca gaaatataag
     2281 tgaaaatata caaacagata aagcaataga aattttaagt gaaaatagac ttttaaattt
     2341 aatggacagg tgcataataa gaaaagttga agctacatta acaaagaagc tttcagattt
     2401 agatgtaaat ttagctgata gtgcatatat agggcttgta gttcatattt cattagcctt
     2461 acagaggata aaaaatggtg aaaatataac tatggataaa gagtttctaa aagaattatc
     2521 tataacagaa gagtttaaat tagctaaaga aatcgtaaaa ggtatggaaa cagattttaa
     2581 catggatata ccagtagatg aggttggata tataactatg catataagag gtgcaaagca
     2641 aaggtcatct tcaaaccata aggctttaaa cttagatgat atagaaataa tggaaattac
     2701 aaataagatg atagatttgg cggaagatga atttaaaata agtttaaaaa atgacgagag
     2761 gttatttaaa gacctagcaa atcacttagg gccttctata aataggttga atatgggatt
     2821 agaaataaga aatcctcttt tagatgaaat aaaaagcaag tattcatatg cctatgatgt
     2881 agttgaaaag atttctagaa ttataaaaga taaactaaat ataaattcaa tacctgaatc
     2941 agaaatagga tatattgcaa tgcactttgc atcagccata gaaaaaaatc ttatgatgaa
     3001 tactaatata aatattgttg tggcatgtcc cactggaata ggtacttcaa gattcttatc
     3061 aactaagatt gaaaataaat ttcctaattt aaatatactt gaaaccatat cagctataaa
     3121 catagatgaa gagtatttaa aggaaaaaga tgttgattta atagtgtcta cagttgagtt
     3181 aaatacaagt ttaaattata tatgtgtagg tccatttatg agtttagatg atgaacagat
     3241 aatcaaggaa aaaataaaat ctatagctca aaataaatta atcaatttaa atgcgaaaaa
     3301 tgatactaag agtaagaata aagtatatga acagataaca gaaagtatga atataggaaa
     3361 agatattctt caatttttag aggaaattag atttgaaaaa tttgaaagta aagatttaag
     3421 tgagcttatt gaagactcat caaaaatatt tgcaaaatct agtgaagata taatctctat
     3481 aaaagaatcg ttaaaagaaa gattaaaaat ttcaattcca tatatagaag aatcaaaaat
     3541 tctattgtta cattgtatga gtgaaagaat agatattatg aagttatcaa taataaaact
     3601 agagaataga atcgtgctag attcaaatga agaaatcgac aatgtagttt ttatgttact
     3661 tcctaaaaat tcaccaagtt atcaaagaca aataatgagt gaaataagtg gttcattaat
     3721 agacaatata atttttacta ataagataaa taaattctct atagaagaga tggcattaga
     3781 ggtaaaagat atagttttta atttttatac aaacagatta aaagcattta tagataaata
     3841 aaattaatat taaaaaggaa ggttgatact atgagtaaag tattgaatga gaataatata
     3901 tttttagggt tagatagtgt ttcaaaggaa gaagctataa ctttagcagg aagaaaatta
     3961 gttgaaaatg gatatgtaaa agaagaatat ataccagcaa tgttagaaag agaaaaagta
     4021 atgactacat atatgggaat gggagttgct attccacatg gtgtaaatga agcaaaaaaa
     4081 gaaatattat cttctggaat agttatatta caattcccta atggtataga ttttgatgga
     4141 gaaaaagcat atttattaat aggaatagct ggtgttggag atgaacattt agaaatacta
     4201 tctaatatag cagtagtttt agatgatgac ttaacagaaa gactaaaaaa ttcaaatgac
     4261 aaacaagctt ttatggaagc ttttgccaac taatcttagg agggatttaa aatgaaaaag
     4321 gcaattcagt ttggagcagg aaatattgga agaggtttta ttggaggctt gttagttaag
     4381 tcgggttatc atgtagtatt tgctgatgta aatgaggaga tattaaactc aataaacaaa
     4441 gataaaaagt atactataca cataagagat gtagaatgta tagatgaagt aattgataat
     4501 ataagtgcgg tttcatctat aaaagaagaa ataatagatg agattgtaca ggctgaaata
     4561 ataactacag cagttggacc tttagttttg actaaaatag cctctacaat tgcaaaaggt
     4621 ataaaggcta gaaaagaaaa gggattaaca agtaatctaa atataatagc atgtgaaaat
     4681 gcaatatacg caagttcttc tttaaaagaa gaggtactta agtatttaaa taaggaagaa
     4741 gtagaatatt tagagatgta tgttggtttt ccaaactgtt cagttgatag aatagttcct
     4801 cctggtaaaa acgaaaatcc tcttgatgta acagttgaaa atttttatga gtggaatgtt
     4861 gaaaaacaag gatttaaagg ggaaatacca actatagttg gtatgaattt agctgataat
     4921 cttatggctt atatagaacg taaattgttt acactgaata caggtcatgc tataacagct
     4981 tatataggat atttaaaagg atataagact atagaagaga gtataaaaga taaatttatc
     5041 tgtgatatag tcaaaagtgc aatggtagaa agtggagaag gattgattaa aaaatacaat
     5101 tttgactcag aagtgcacta taaatatatt gataaaattt taaatagatt taaaaatcca
     5161 tatcttaatg atgatgtatt aagagtcgga agggaaccat taagaaaact aagtgataaa
     5221 gatagattga ttaaaccttt aatgacagca aaatcatatg gattatcagt tgataatttg
     5281 attcttggaa taggtgcagc gcttcattat aataatagtg aagatactca aagtgtagaa
     5341 ttacaagagt tgataaagtc tattggagta aaaaaagccg ttgctaaaat agctaatata
     5401 agcaatgacg aagaactttt aaacaatatc gaaaaaagtt atatatttat gaaaaattta
     5461 taaatttact tgaaaaaaag tagttctgat aaagtaacta taatatatta ttctacacag
     5521 tttgaattat ttgttgaata atagagaatt ataatatata attaaaagga ataaattaat
     5581 atatttacct gtatatgttt gataatatgg ttcaaatgtt tctactaagc taccattaat
     5641 agcttgacta caggagtgaa gaagatatag tttatactta ttttaaatta tatattaagt
     5701 taattacttt gaattttaag ctcctgtcta tgatatagac aggagctttt ttagtatttt
     5761 aatataaaat acttacaaga atagatagca aaattagaac aaatataatt tttttattaa
     5821 tataatttta gaaagggcta ggtggaagtt caatgaaggc tttacaagaa aagatattga
     5881 gagaaggcag tgtttcagga aatgatatac taaaagttga tagtttttta aaccatcaaa
     5941 ttgatgtagc ttttttaaat gaaataggaa gagaatttaa agagagattt aaaggagaaa
     6001 aggtggataa gatatttaca atagaggctt caggaatagc tatagcatca attgtttcac
     6061 agtattttga taatgcacct gtagtatttg caaaaaaatc agaatctaaa aatttggata
     6121 aagatgtata tgaaactaat gtatattcat ttactaaggc aagagagtat tcagttaaag
     6181 tttctaaaaa gtacataaac aaaggcgaaa atatattgat agtggatgat tttttggcaa
     6241 atggaagagc tgctcttgga cttaaagatt taattgagca agcagaagct aacttagttg
     6301 gagtaggaat tgtcattgaa aaaggatttc aagctggagg agcactttta aaggctaatg
     6361 atgtaagatt agagtctttg gcagtagttg aatctataga taatggaact gtaaaattta
     6421 ggtaatttat gatattatat acttgtgttt agcacatatt ttaactatgt aattatggag
     6481 gattttttat gagatttttt atagacacag ccaatattga agaaataaaa gaagcaaatg
     6541 atttaggtgt aatctgtggt gtaactacta atccgtctct aatagctaaa gaaggtagag
     6601 attttataga ggtagttaaa gaaataagtg aaatagtaga tggaccaata agtgcagaag
     6661 taataagttt agaacataaa ggtatgatag aggaagcaga aaaattatct aagatacata
     6721 aaaatatagt tataaagata cctatgacag cagaaggtct aaaagcggtg aaagtactat
     6781 caagtaaagg tataaagact aatgtaacgc ttatattctc tgctggacaa gctcttttag
     6841 ctgctagagc tggagctaca tatgtaagtc cttttgttgg tagattggat gatatatcac
     6901 aaaatggtct tgatttaatt gaagaaatcg ttgatatatt tagtgtaaat agtattgagg
     6961 ctcaaataat agttgctagt gttagaaatc caatacatgt tttacaagct gctagaatgg
     7021 gagcagatat agcaacagtt cctcttaaag ttataaacca aatgattaag catcctttga
     7081 cagacaaagg aatagatagc tttatgaagg attgggaagg tgcttcttta aaattataat
     7141 tataaactac gctttataag tatattaaag aggttattat ggaaattcta agtaataagg
     7201 ctaggctatt ttgcctagtc ttattttggt gtataatata ttgaaacata ttattgaaat
     7261 agattaattt tttaagtgag gaggcgaaaa atggataaaa tattaagaga gatacaaaga
     7321 gaagataaaa aaaatccata tactgaccaa gaacttgctg aaatgttaaa tgtagctagg
     7381 tctgaagtga tatcagttag gaaaaaaaat aatattttag attcgagaga aagaagaaaa
     7441 aaaatattaa taaaagatat atcaaaaatt gtaagtgaaa atcctcaaat gtctgaaaga
     7501 aaaataactg aaatattaat agaaaatgga tataatatat cgagaagtgc agtttcaaaa
     7561 ttattaaaag aggaaaattt aaatcagtca aagattggga ttaaggataa agacccagca
     7621 tcatcaggaa agtatgtgaa ggtgagtaaa gttgtagaag aagatggatt tgaagaattg
     7681 ataggtataa agggaagttt gaaagaaaag gtaaatctag ctaagtcagc aataatgtat
     7741 cctcctaatg gccttcatac tataatatat ggagaaacag gagtaggtaa aagtgaatta
     7801 gctacatgta tgtataaata tgctataaaa aacaacatta aagaagagaa cagcccattt
     7861 atagtgttta actgtgcaga ctatgcagaa aatcctaatc tattgatagc tcaactcttt
     7921 ggggtagtaa aaggtgcata tacaggagca gatgcaaata gagaaggtct tgtagaacaa
     7981 gctaataatg gaatattgtt tttagatgaa attcatagat tgcctgctgc tgggcaagaa
     8041 atcttatttt cattaataga tagaggagaa tttagaaggt taggagaaag tagttctaat
     8101 agaaaggcaa atgttctcat aataagtgct acaacagaaa atccagattc taatttactt
     8161 caaacattta gaagacgtat accaatggta ataaatttac ctaatatgag tgaaagacca
     8221 aaatctgaaa gatatgaaat aataataaag ttttttgaaa gagaagcaaa gcgagtaaac
     8281 aagaatttta taataactaa agaagttatg tgtgcactta tgaattataa gtgtactgga
     8341 aatataggtc agttaaagag tgatatacaa gtaacttgtg ctagagcttt tagtaaatcg
     8401 atattttcta gcgataaagt aattatagat ttagatagtt taagggatta tataaaaagt
     8461 ggatattatg attgtaattg taaaaatgaa aagtattctg agttttcagt tgaggatata
     8521 tatatagata ttagtgaatt ggtgagtgga aaaaatagaa ttttatctga taatgagata
     8581 agcgagatat ataattatgc agaaaaagag cttaaggttt tggaaagtaa gtgcttttca
     8641 gaagaagagt taaaaaatgc atttatagaa aagttagata agaagtttaa tgctattaaa
     8701 aacaataaaa atttatctga tagaagaagt cgaatagact ggggaggaca attaaaagag
     8761 tctacactgg agataatgga taaggtaatt gtatttatta aggagcagtt taagcatgta
     8821 aatcaaggtc tgtacttggc tctagcaatt catattgagc atgctatagg taggataaaa
     8881 gatggaaaaa ctataataaa tccgagttta gataaaataa aagcatcaat gccaatggaa
     8941 tatgagttgt caagatatat aatgggtata gtagaagatt tgacacaagt aacatttcca
     9001 gaagatgagc ttggatattt ggcttattat ataaataaat tttgttataa tgaggaaagt
     9061 ataaaagata aagttaaagt agtaatagtt acgcatggta aagttggaat tgaaatgtct
     9121 aaagttgtaa atcatatttt aggtatagaa tgtacactag gtatagaaat agcacttaca
     9181 gattcaccat cagaggggat agagcatgta ttagaagaac ttcaaaagat agaagctaga
     9241 aaaggtattc tagttctaat tgatatgggt tctttggtaa ttcttggtga tgaggttgaa
     9301 aaaaggcttg gaattagatg taaaactgta aatagagtag atacattatt ggcaatggaa
     9361 gcaggaaaac ttgcaacaat tgaaggaaaa agtttagatg gtataatagc tgatttaaag
     9421 aaaaataaga attatgctat gataaataca aataaattta gttacagaaa aaacgagtat
     9481 ggaaagaaaa atgtaatcat tactttatgt ttgagtggtg taggtacagc tttaaattta
     9541 aaagagcata tagaaaaaca aattaaggaa tatgacactt taattgaagt aaagccagtt
     9601 gcttttttaa ataataattt agaagaagag ttaaatgata ttgaatctga atataatatt
     9661 attgctatct gtggaactat tgatatagat tataaaaacg tgccttttat ttcttataat
     9721 gaggtacttg gtaaaaatgg tattaataaa gtattagagc atttaaatag taaacaacca
     9781 gctttaaata ttgaaaacca gttgaataat ttaatacatg aagatttgat tttatacaat
     9841 tttgaaggaa tttcaaagga gtatataatt gatactcttg tttcaaaact tgaagaaggt
     9901 ggatatgttg actcaaagta tatattaagt gtatataaaa gagaagcaat gggaagtgtt
     9961 gtaatggcct ctaaggtagc cgtgccacat ggacttccag aaaacgttat gaagcctgca
    10021 atagcaatcg cgaggttgaa taaacctata gtatgggaca ataaatttat ggtggatttg
    10081 gtggtactac tggcacttaa agaaaataat aagaaagaaa tacgaagctt attttcaaag
    10141 attaatgacc agcatacttt agaagtttta ttaaatactg aagataaaaa tgaaataaaa
    10201 gaacttttaa gctaattatt agtaccgaga agaaagacaa aaaaatttgc tttagtataa
    10261 aatttaaaac tactcaattg ctaagtagtt aattactcta tataaggcta gttctatgaa
    10321 atttatttat atgat
//
LOCUS       DAMJZE010000145        10275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJZE010000145 DAMJZE010000000
VERSION     DAMJZE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.149"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            338..1768
                     /locus_tag="PC541_002944"
     CDS             338..1768
                     /locus_tag="PC541_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2840388.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1871..2974
                     /locus_tag="PC541_002945"
     CDS             1871..2974
                     /locus_tag="PC541_002945"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2840389.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2989..3225
                     /locus_tag="PC541_002946"
     CDS             2989..3225
                     /locus_tag="PC541_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2840390.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3242..4291
                     /gene="vorB"
                     /locus_tag="PC541_002947"
     CDS             3242..4291
                     /gene="vorB"
                     /locus_tag="PC541_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2840391.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4293..5033
                     /locus_tag="PC541_002948"
     CDS             4293..5033
                     /locus_tag="PC541_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2840392.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5036..5575
                     /locus_tag="PC541_002949"
     CDS             5036..5575
                     /locus_tag="PC541_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2840393.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5699..7798
                     /locus_tag="PC541_002950"
     CDS             5699..7798
                     /locus_tag="PC541_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2840394.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8065..8580)
                     /locus_tag="PC541_002951"
     CDS             complement(8065..8580)
                     /locus_tag="PC541_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2840395.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8672..9883)
                     /locus_tag="PC541_002952"
     CDS             complement(8672..9883)
                     /locus_tag="PC541_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2840396.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3895 a         1249 c         1870 g         3261 t
ORIGIN      
        1 tgaataaatt attcttgaat aaaattataa aaaattaaaa tcactagaaa tatgataaaa
       61 taatttaaaa tacgcatttc aatgacattt caaaacaaga aaataataaa aaacaaacct
      121 aaattaagtc taaatagctt tttagttaat tttagtagta aaaaataaaa cttaaaaagt
      181 gtttgacaat tatgaatttt gagaatataa ttaaattaaa attatacaca tataaaaata
      241 tactcctgaa tcaagaatga gatatttaag attcaaaata tatattatta tacgtgaata
      301 ataaaaagta agtaaatttt ataagggagg ggtagatgtg agtagtcaag aaagcaaacc
      361 aaaaggaaat cttatatcaa attttagaga tgatatgaga gaccctaaga aaagagcaat
      421 atacacatat tcaagttcta taaatttagc gtacaatttt ataggttact ttgtatcaac
      481 tgttacaaat atttttttaa ctgatgtggc aaaacttggt gtaattatgg caggtggtat
      541 gcaaattata caatctttca taaaaattgc tgtagctcct ataacaggta cagtatttga
      601 caaacaacca ttcaaaaaag gaaagtatta tccatggctt agatatgcac cagcagcgtt
      661 agcattaaca tatggaatat ttttcatagt tccattaatg aaaataagtc cagcattact
      721 tttacctttg atgacagctt tattattaat agcatcttgt gctcagcaag tagttttcac
      781 tataatttat gctatttacc ctgtagttgc taagacacct agagaccgag ttataggctc
      841 aactactacg aatatattta aagaagttgg taaattctta gtaggttttt catatccgtt
      901 attattagtt ttatttgcag atgtattagg tggagaagga atgggttatt taggtaccta
      961 cttattcttt gcagcaggat gccttttagt attttggttt tcttcaacag agataataaa
     1021 atctggagca gaagaagaag ttcttcaaaa agaagagaag ccaaagatta aagcaagtga
     1081 aatggcaaaa gctttattca ctaatgtacc acttatgatg gcattttgtt tagaatttct
     1141 tatatgtata agatctatag gattaggacc tttggctcca tattatttta aatatgtaat
     1201 agaagatgaa cgtggattag cagtattttt atcaatcatg cctttggtaa gtgtagcatt
     1261 catgtttttc gcaccagtgt ttataaaaat ctgtagagag caaaaactag cttcaataat
     1321 ttctttttct atatgtgcac tatgccactt attggttgca tggactcctt gggggaaaac
     1381 tactttagga gtaactatgt tgttggcaat tggtggaggt ttttcaaatg tagtatcaat
     1441 aataaatctt aatttctttg ccggttcttg tgattatggg cattggaaat caggaaaaga
     1501 cttacctggc ttaagtatgt cactatatcc agttgcaata caagtaggag tattacttgc
     1561 aactactata agaacagttt taatgaactc aatgggatat caagctgata tggtagtcac
     1621 agaagcagtt aaatcaggtt ttataaatat gatttcttat tctatggcaa tacctttaat
     1681 aatagctgtt gttatagcaa tactatatcc agtaagtgac aagaaattaa accaaataag
     1741 agaagagtta aatcaaagaa atgcttagtt aaaagaaagt tgagtagatg gttaatttaa
     1801 gctattaaag taagtagacg caatagcgtt tatataaatg aaaatacata tacagagggg
     1861 gcttttaaaa atgatagaaa aattattgag aaataccgta aaagaattac atcaatatgt
     1921 tccaggtgaa ccaatagaaa aagttaagga aaaatatggt gtaaaagaaa taataaaatt
     1981 agcttcaaat gaaaatcctc taggaccatc accaaaagca atagaagcta tgattgaaat
     2041 gttaaaacaa ggtcaacttt atccagagcc agaagctaac gaacttagaa gaaaattagc
     2101 tgaaaagcta gatttaaaac cagaaaattt tattgttgct aatggtgcag ataacgttat
     2161 cactctaata ggtgaggctt ttataaatag aggagatgaa gttatatatt gtaacccaac
     2221 atttccatca tatagaactg caactataaa aaatgaaggg ataccagtag aagtaccttt
     2281 aacagaagat tataagtatg atttacaagg tatattagat aagattacag ataaaacaaa
     2341 attaatatgt gtatgtaacc ctaataatcc aacaggaaca attgtggatg ataaagagtt
     2401 agaagaattt ttaaagaaag tccctgaaaa tattataact atattggatg aagcatatat
     2461 agaattttta acagttccta attatgtgga tggacttaag tatgtaaggg aaaattataa
     2521 cgttatagta acaagaactt tctctaaaat atatggtctt gcagggctta gagtaggata
     2581 tggtattgct aaagatgaga taataagaac tttatttaca gtaaaagagc ctttctctgc
     2641 aaatcgtgtt gctataagtg gagcaacagc agcacttgat gatgaagagt ttataaaaga
     2701 aacatatgaa ttaaatagag ttggaatggc ttactttaaa gaagagttta caaaaatggg
     2761 atttgatgtt gtagattcac aatcaaattt cctttatgta gatatgaaaa cagatatacc
     2821 taagttattt gaagacttga aaaaaagagg gtttgtaata agaccaagtg ctacacatgc
     2881 acgtgtaagt attggaacta tggaagaaaa taggaaattt gtagcagttt taaaggaaat
     2941 attaaatatt aaaggagagc caagtattgg ataatagata gaaatattat gaaacggggt
     3001 gtagatatga acttaaaaat aaacgcagag agatgtaaaa gttgtgaata ctgcgttata
     3061 tcttgtaaaa aaggagcatt aaaaatatca agtaagataa ataaagaagg ttatgctcat
     3121 gtagaagttg atgagagtaa atgcatactt tgtggaattt gctatcaggt gtgtccagat
     3181 aatgtttttg aaataattaa agaagaagca tgtcaagagg cttagaagga gttgggataa
     3241 tatgaaaaag atgtggaagg ggaaccacgc cattgcagaa gcagccctaa gaggaggttg
     3301 tgaattttat gcaggatatc caattacacc acaaacagag gtaatggaat ttttatcaca
     3361 tagaatgtct gaacttggaa gaacttttat acagtccgaa aatgagatgg ctgctatata
     3421 tatggtatat ggagcatatg caagtggaat gaggtctatg acgagttctt caggaccagg
     3481 aatatcttta aaacaagaag ggatttctta cttatgtgca aatcattacc cttgtgtaat
     3541 cgtaaatgta cagagatggg ggcctggtct tggctcattg gactcagcac agacagacta
     3601 tctaagagat actagagggg gaggaaatgg agattatcat ttgatagttt atgcaccaaa
     3661 cagtattcaa gagactgtag atttgatgta taactcattt gatgtagcgg agaagtacag
     3721 agtgcctgtc gaaattttga cagaagcagc tttaggtcaa atgatggaac cagtagaatt
     3781 tccagagttt aagaagagag aaggagattt aggttggact tatgatggaa gtaatagaga
     3841 ccatgcaaag gttgcagata accaaaagcc aactttttgt atggagaaac gtatgagaat
     3901 aactgaaaat gaacaacaat gggaagatta tcaagtagaa gatgcagaat atgtgtttgt
     3961 agcgtttggt ataccatcaa gaacaactat gaatgctgtt agaagactta gagaacaagg
     4021 tgaaaaagtt ggaataataa gaccaataac agtatggcct ttcccatata aagcatttga
     4081 aaaggtaagt aaagatgtaa aaggatttat ctcagtagaa tcaacagaca caggtcaatt
     4141 agtagaagat gttgctttag cctcaaagaa agtatgtaaa gaaaatgtac ctgtgtatgg
     4201 tttatttagt ggaaatcata taccaaaaac attgcaagta atggacactt atagcaaaat
     4261 aaaatctggc gaaatcaagg aggtgttcta atatggctac agaaaaatta acacctagcg
     4321 ttgtttcaat accaaacaag ttttgcccag gttgtggtca tggaatagta aatcgtataa
     4381 tagcagaggt aatagaagaa catggatatg aaaaaaatca tgtattaaca ttaggagttg
     4441 gttgtgtctg caatatgaat tttagctgga atggtgataa gatgcaaact gcacatggta
     4501 gagcttcatc tacagctata ggagttaaag tagcacttcc agatacatta gtaatgactt
     4561 accaaggtga tggagatgct tatgttatag gtctttctga aactttaaat acagcatata
     4621 gaaatcataa tgtgactgta tttgtaataa acaataataa ttttgcaatg actggtggac
     4681 aaatgtcatg gaccactatg ccgggtcaag tcacaacaac aagtgtaaat ggaagagata
     4741 taaagactac tggtagtcca ctgaaagttc ctgagatggt ggcaaacttc tttgatgtag
     4801 catatgtagc aagaggttca gttcatagcc caaaggaaat aattaatcta aaaaaatata
     4861 taaaaaatgc aatagaagct caattaaatg gagagggata ttccttagtt gaaatattag
     4921 caccttgccc aactaactgg ggtgtatcac tagagaaatc tatcaaatgg atggaagaag
     4981 aaatagtgcc atattatgct cttggcgaat ttaagcaaag ggatggtgaa tagttatgga
     5041 aaaagagcta gtatttgcag ggtttggtgg tcaaggagtt ttaactcttg gtctgattgt
     5101 agctgagtca gcacttgaat tggggaaaca agtaacttgg atgcctgcat atggtccaac
     5161 tatgagaggt ggaaaagcct atagtgtagt aaagttttct gatgagagta taggtgggcc
     5221 tgatatggaa gaaatagatg ttttagtagc aatgaataaa ccatctttag attatataaa
     5281 tttagttaaa gaaggtggaa cagtagttat taatacatct gcaatagatg agaatgtaac
     5341 attaagagaa gatataagtg tagttaaaat aaattgtcag gaattagctc aaaaagtaaa
     5401 caatccaaaa gcagcaaata tagtagtact tggagcatta attgctaaaa ctgggttatt
     5461 tgaaaaagaa ttagctttaa aaactatgtg tgatttcttt gaggaaaaag gaaaaggaaa
     5521 gtttaatgtt caaaatgaag cagcattcat ggaaggctat aataacgcca tttgataaat
     5581 tacccttatc ttaagtgatt tatctcaaga tttaataatc tttagtgcca agagaaactt
     5641 catcttgaga taaaattcac ttaaaatcac aactataaac atataggagc gtgaatttat
     5701 gagtttggaa aaacttatta gaccaaaaag tattgctata gtaggagtga ctgataaact
     5761 tggttttgga agaagtgcag cacttagtat agtaaaaagt aaggaaacag atagagtata
     5821 ttatgttaat cccaaaagag aagaattgtt tgggagaaaa tgttataaga cgatacaaga
     5881 agttccagaa gtagtagact gtgtagtagt atgtacgcct agaaatgtag ttccatctgt
     5941 attaaaagat tctggagagt taggaacaaa ggcagcagtg gtatatgcta gtggatttgc
     6001 agaggaaggt acagaggaag gtacagattt agaaaatcaa cttatagaaa tatcaaatac
     6061 atacgatatg aaaatattgg gtccaaactg catggggttg ctaaattgta tagataaggt
     6121 taatctatgg gcaggaggtt ctaaatggga tttagatact aaaaaacctg gtataggaat
     6181 tgtaggtcaa agtggattta taactgctga aattgtttca agtgattatt ttaatatatc
     6241 ttatggtttt tcaacaggaa atggaaatat agttacacta gaagatgcag tagatttcct
     6301 tgtagatgat gattatgcat cagtaatagc aatttacctt gaaggtttaa aaaatcctca
     6361 aaaatttata gatgctttaa aaagggcagc acaaaaaaga aagccagtaa taatattgaa
     6421 atcaggaagg tcagaaaaag gtgctatttc tgctgcttct catacaggaa atttagctgg
     6481 ttcgagtaaa gcttttgaaa gtatttttga aaaatatgga gtaatttctg tggaaaattt
     6541 agaacagttt atgtgtttag cacaagcttt tagtgtttta gatggtaatt tacctgcaaa
     6601 ttctaatttt gcagcaataa acttctcagg aggagaaaat actatttgtg cagatttagc
     6661 agaagaaaat ggtgtagaac ttgcagaaat atcttctgaa actaaagaag aaatggaaaa
     6721 atatcttcca ggatttgcaa cacctaaaaa tccactagat gctacaacag cattattcca
     6781 tgaaaaggat atgatagtag gtttattaca tacctttgaa aaggatgata gtgttggatt
     6841 tactatggta ggtgcaaata taagagatga agaaaatgaa atgcatgaaa ccctttgtaa
     6901 agctgtaagt gaagctagag aacaaggatt gaaaaaacct gtgtttgcag ttcctacact
     6961 tgaaggaaat agatatttag attataggac tagattagaa gataatcaag tgcctataat
     7021 gtcatcagtt ggaactacat ttacttgttt taataaaatg gctaagttta tagattatga
     7081 ttattcaaaa agaactttag aatttaaagc tgttaagaaa agagagtcaa ataatgtagt
     7141 agcattatca gaattagata gtaagattga gatgaaaaaa tatggagtac cagttccagg
     7201 acaaggtaat gcaaaaagta ttgatgaatt agatgaaatc ttaaaatata ttaagtatcc
     7261 tgttgtttta aaaataaatt cttctgaaat attgcacaaa tctgatgttg gaggagttaa
     7321 aataggaatt aagaatagag atgaggcagt agatgcatat aatgagattt taactaatgt
     7381 aaaaaaagca aaaccagatg ctaatattga tggtatatta gttcaagaaa tggtagaaag
     7441 tggtatagaa attattattg gtataactaa tgatgaccaa tttggaccaa tgttattagt
     7501 cggtttagga ggagtatttg tagaggtatt taaagatact acattatatc cattacctat
     7561 aaatcatgat gaagctatta tgatgcttaa aaaactaaaa tcatttaagc ttttaaatgg
     7621 ctatagagga agtgagccat gtgatattga tgctcttgca gatatgatgg tcaagttagg
     7681 aaaatatgct tatgaaaata aggatgaagt caaggaaata gacttaaatc cagtgtttgt
     7741 atatcctaaa ggaaaaggcg tatgtgcagt agatgcctta atagttaaat ataaatagtt
     7801 tataagtttt ggtattcaac cataattaaa gcaccaactg aaaatcacat tttgtaagtt
     7861 agatagtgtg attttcagtc accccaaatt taatttaaaa atattttaaa atgaaagtat
     7921 agttttaagt ttcattgtga aatagcactg tataaacaat aaaatactac gtataaaacg
     7981 aataataaaa ggtgttttaa tgataagaat caatcttgat tttcattaaa acaccttctt
     8041 tattatatta aaaattttta aacgttatat tgtcacaggt gggtttgttg gagctgcata
     8101 tgtacgatta gcaaattctt ggtcaacaaa gaatagccca ccctctccag caagtttaac
     8161 acgttttata ttgtcttcac aatttgtttc ttcttctgct tgttcagata taaaccaaag
     8221 taagaattga gaagttctaa agtctgattc agagtttgct acagctgcta agttgtttat
     8281 taaagctgta accttttttt catgagctaa cgtacgctca agaatatcca ttgcagaagt
     8341 aaagtctgaa tctggcattg ggatagcatc aagctgaacc tttcctccta cattatgtaa
     8401 gtatttgtaa aaaaacatag cgtggtctgt ctcttcttta tattgaacat aaaaccaatt
     8461 agaaaatcct ttaagtcctt ctgcttcaag gtaagaagac atagaaagat aaagatacgc
     8521 agaatataat tcatggttaa tctgctcatt aagtaatttt tccattttag ctgaaatcat
     8581 ttttatacct ccaaatttta aatgctctta taaaatataa taatattaaa tggaaaaata
     8641 ttcaataaaa gtgtttaaat attttacata tttaataaaa atttatacta ccattaaaaa
     8701 aattataact tacaatggct atagagccaa ttaatggtgc atttgctcca aaatttgaat
     8761 gccttacact tattttttta tatttagaaa aacttgctga agcagtcaat ttttttaata
     8821 atgtattttc tataaatgta ccaggtatat taaagtcata gcctattatt atagaactac
     8881 aatcaatcaa tgtaagtgta ttaactaatg catatgatac gtatgtacaa aattcatcaa
     8941 gcaaaaacat ggcaagacta tctttattat ttgcctcatc tattaacttg actaaagaaa
     9001 aatctttaca gttaattaat ggggagttag gataaatatg gctcaatgat tcaattcttt
     9061 taattagatt actgacactt gtataataat ctaaacatcc attgttacca caactgcaca
     9121 atggtccact aaaattaatt gatgtatgtc ctatttctcc actttgtcct aggttgccag
     9181 tatgaagttt attttctaga actagaccag ctccaatacc attcattatg tgtaggtata
     9241 tataatttga tatattttta cctaaaccat acattttttc tgctaatgca cccgaattag
     9301 catcatttat caaaaatatt ggcaaatcag acatttcttt tataaaatct actatattta
     9361 ggtttgatac atttccaaaa tctgggggat ttagtatggt accatttata tcatctactg
     9421 gacctattga agaaatacca attccaatta tatctctctt gttgattttc attattttag
     9481 taaacattct ttttattata tcttttaaaa catcatttga tagaaattta gggtaagttt
     9541 cagaaagttg ttttacaata ttacctttta aatctgaaat aattacctta catatatttc
     9601 ttcttattag gattccacaa atacaaggcg atatattaga aatatctaat aaaataggtt
     9661 tacgacctga tgaatgtgca tttaaagttg atgactctat ttccctaatc atattttcat
     9721 taataagttc agtaactaga tttgataaag tcattttagt aagtcctgta gacttagcta
     9781 aatcaactct ggacataggt gtatttgtag aaagtaattt caatattatt aacttatttt
     9841 taatttttac atctttgatg ttagaacctt gtttgttact catcataaat ctccttaaat
     9901 aaagttaagt gttttattta ttttcaagta tactatcacc ttataataga gtaacatttt
     9961 tttacttgaa aatatcatta attagaatat atcacattat atatcataaa acatataaaa
    10021 aaatattaga attaaattaa aagtgaaaag tagattgata tataaatagt aaatacttaa
    10081 ttaaaaaaag ataatgatag caataattat tgttaccaag aatctactac ttcaagtgtt
    10141 accttaggta tttaagttga tagtggtttg cacgtaataa acttgatgta atatatattc
    10201 ttaataatat cgataaaaga tttacttaaa aataatatag attatgtaaa catataatct
    10261 atttattgta tacaa
//
LOCUS       DAMJZE010000146        10212 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJZE010000146 DAMJZE010000000
VERSION     DAMJZE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.150"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..882
                     /locus_tag="PC541_002953"
     CDS             <1..882
                     /locus_tag="PC541_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2840397.1"
                     /translation="FLIKEKYKYILFSFVIISSLIISLDKNIYLALESSSILLIITVL
                     SIYIENENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREI
                     HDSVGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEY
                     ENYQSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHG
                     KATEIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNG
                     FTIRASFPKFSGGDFFE"
     gene            875..1522
                     /locus_tag="PC541_002954"
     CDS             875..1522
                     /locus_tag="PC541_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840398.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1651..2586
                     /locus_tag="PC541_002955"
     CDS             1651..2586
                     /locus_tag="PC541_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840399.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2600..3697
                     /locus_tag="PC541_002956"
     CDS             2600..3697
                     /locus_tag="PC541_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840400.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3700..4764
                     /locus_tag="PC541_002957"
     CDS             3700..4764
                     /locus_tag="PC541_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840401.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5008..5505
                     /locus_tag="PC541_002958"
     CDS             5008..5505
                     /locus_tag="PC541_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2840402.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5681..5929)
                     /locus_tag="PC541_002959"
     CDS             complement(5681..5929)
                     /locus_tag="PC541_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840403.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5995..7011)
                     /locus_tag="PC541_002960"
     CDS             complement(5995..7011)
                     /locus_tag="PC541_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2840404.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7069..7836)
                     /locus_tag="PC541_002961"
     CDS             complement(7069..7836)
                     /locus_tag="PC541_002961"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2840405.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7904..10093)
                     /locus_tag="PC541_002962"
     CDS             complement(7904..10093)
                     /locus_tag="PC541_002962"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2840406.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3550 a         1574 c         1187 g         3901 t
ORIGIN      
        1 tttttaataa aggaaaaata taagtacata ttgttctctt ttgttataat atcttctctt
       61 attattagtt tagataaaaa tatatatttg gcactggagt caagctctat attgctaatt
      121 ataactgtac tttctatata tatagaaaat gagaatttat caaaattata cagtcaaaat
      181 ctttatgata agcttagaat ttctaaagat gaattaaaaa aagttaatgc tgatttggaa
      241 acttatgcaa gttctataga agaattagca attttaaaag aaagaaatcg aatatcaaga
      301 gaaatacatg atagtgtagg acattctctt tctacaacaa taatacaatt aaatgctata
      361 gaaaaactct taaaagacaa acctctaatt tatgacttag tacatgaact tagagaattt
      421 gtaactgaaa gttttcaaga tgtaagaagg gctatatctg agttaaaacc tgttgaatat
      481 gaaaattacc aaagtttatt taaaatcaaa gaattagtta aaagctttat taagcttaca
      541 aatattaatg ttaaattaac tatttcaaaa aatacttgga acttatccag aaatcaatct
      601 atagctttat atagacttat acaggaatcc ctttccaact catcgatgca tggaaaggca
      661 actgaaatca gaatttttat tacattcaat acttcaaatc ttataattac tattagtgat
      721 aatggtatag gatgtggcaa tataaaaaaa ggaaatggac ttaatagtat caatgagaga
      781 atatatgaat taaatggtaa agttgaattt gataattcta ataatggttt cacaattaga
      841 gcttcttttc ctaaattttc agggggtgat ttttttgaat aaaattaaag ttcttatagt
      901 agatgacgaa aaattaatta gaaaaggtct taaaattata ttatcatctt ataatgattt
      961 agaaattgta ggagatgctt ctaatggata tgaagcttta gaattttgta aaatgaatga
     1021 tgtagacata gttcttatgg atatacgaat gaaagtatgt gatggggttc taggtacaag
     1081 gcttataaag gaatataata attctataac attattaatt ttaacaactt ttaatgatga
     1141 tgaatatata aaagatgcta tgaagtttgg tgcttcagga taccttttaa aagatagctc
     1201 tgatgaagta ttgcatgaag ggattcgttc ttcatttttt ggcaatattg tgcttgataa
     1261 aagtgtagct gaaaaaatca tgacctctga aaaaacgatt aaacaagagc atctttatga
     1321 catgtacaat ttaacagaaa aagaaatatc tataattaga ctaattgcaa atggacttaa
     1381 taataaagaa ataagtcaag aactattctt atctgaaggt acaataaaga ataacataac
     1441 taatatttta gctaaactag aacttcgtga cagaactcaa ctagcaatct ttgctttcaa
     1501 aaataaaatt gtaatagaat aaatctaaaa atataaagtg acttaagtca ctttttttag
     1561 tgacttatag taatagaatt ataattcaca attctttatt ataatattat agctttttaa
     1621 cttatacaaa attattttgg agggattact atggatatag tcagagtaaa taatataaca
     1681 aaaagattta atgataaatt agtacttgat aacatcagct tttctgttaa aaaaggtgag
     1741 atatttggac ttattggccc aaatggtgca ggtaaatcta cacttataaa tataattaca
     1801 aatcttatgt taccaaatag tggtagtatt caaatcaatg gtttagattt atcaaaagat
     1861 tatataaaag caaagtctat aatagggtta gttccacaag aattagcaat tatagaaact
     1921 cttacccctt ttgataactt agagtacttt ggagctttct acggtttaaa gggaaaacta
     1981 cttaaagaaa gaatcattga agcattagaa gttacaggtc ttactgaagt caaaaagaaa
     2041 aaggtcaaaa aattatctgg tggtatgcaa aggagattaa atattggaat agcactctta
     2101 aatcatccta aaatattaat attggatgaa ccaactgtag gtgtagaccc ccaatcaaga
     2161 aatcatattt tcaattttat aaaagatata tctaaaaaac atgaaactac tgtaatatat
     2221 acttcccatt atatggaaga agttgagcac ctttgttcta aaatttttat tatggatgaa
     2281 ggtaaagaaa ttgcttttgg aaataatgat tatttaaaat cactcgtatc tacaaataca
     2341 aaattaatta tggaaataaa aaatataaat gcccaactta tctttgattt aaaaaatact
     2401 aaaggtgtta tatctgtttt agaaaataat tcattattag aattggatat agataaaaaa
     2461 ttacaactta cagatatact ctctgtcatt gacaaaaaca attcaaaaat aatgaaaatt
     2521 tcttatgaag aacctagcct agaagacgta tttttaaatc taacaggaaa aaatttaagg
     2581 gactaatgga ggtgatttta tgaaattata catgagtgta aaaactatgc taaagagttt
     2641 aaaaagctcc tttatactta atttaatata ttttttagct ttgcctctga ttttatcttg
     2701 gtttcttggg atggttacag aaagcatgtt tcaaaaccct ataaaaacag aatctacccc
     2761 cattgttata tatgacaaag ataacacaag attatcaaat gatttgacaa agtatttgaa
     2821 aaatgattta tcttatatat taacagttaa aaaagatgat tctaaggcag aattaaagct
     2881 tactatacct aaaggctatg agagtagttt attaaatgaa aaatcaaata ctctaaacat
     2941 agaaaaactt ggaactcgtg atgatattgc aatattgctt caagatatat tagatactta
     3001 ccatgaaaaa ttttatttaa ataattctca gaaaataagt tcagaagatt tttctaaatt
     3061 atttaataaa aattctattg atacttctat aataaataac aatgttaaac agagctctta
     3121 tgaatacttt gctttagttt cactaggatt tcttgtaata atttttataa tgaacaatat
     3181 tttgtcaaat tatataagtg aatcaaaggg actttcaaaa agactttatt ctatgcctat
     3241 aactagagta caattcttaa tttatgactt tgtaggttta tggatttatt catttatatt
     3301 cttattatta tatgttttat tctttagaat acttggaata acatttaaag gtaattttgc
     3361 catattgctc ctactttgtg ctttatcatc ttattttatg acttcaatat ctacctttgt
     3421 aaccagcttt tttagtaaaa aatacggtac agcaatagtt tatgcattac tctttttaca
     3481 gacaattttt ggaggtatct tcagtatgat aagtgataca tttactaagc ttacaagttt
     3541 atctcctacc tatttaatag gagaattgtt tagtaattat gaaacattta aaaccataga
     3601 ttcaatagga aatttaattt tcacatgttt aattatctct acaatattaa ttgcacttgc
     3661 tattgtaaag gaaaaatata aatggaggga ggtttaataa tgattagatt aactattgca
     3721 aatataaaaa gatatctaaa aaatcatact cttttaatta atatggttat gcttcctatt
     3781 atactaattt tttcacttaa tttttttatc aacaactcgg gaaatcaatc aatgtacttt
     3841 tctccagtag caattgtatc tgactcttca ggaaagtatg aacataagtt aataaatagt
     3901 tctaaattaa aagaaaattc atttagatta aatgaacaag ataaggctat gaatctatta
     3961 aaaaataata aggtttctgc tgtattcgtt ttagataaaa atttttcaaa tagtatagat
     4021 aaattacaac gacctgtagt taaatgcttt aaaattgaaa atggtggtgg tagtctttgg
     4081 gcagaatcac aaatagaatc ttttattact aaatcgttaa aattgaaagt ggataaaaat
     4141 atagatgata aattgacaaa gacaaatata attgataaca aaccagataa gaataaaggt
     4201 tcatttttag tagtcttctt gatatgctat tttatgtata ttaatgcggc acatcttgct
     4261 tctgatttat ttacattaaa aaaatcaaat gtgctaaaaa gacttatctc tacagataat
     4321 aaagatataa aaataatttt cagtatattt ttaggactat tttttattca atcaattgta
     4381 tacacttttg ctctattatg tttttcaatt atagaagact ttaatttgag tctgaatgta
     4441 ttgatgattg tactagcaaa tagttttgtt tccactggat ttgtattttg ggtagctaga
     4501 gtatttaaaa atgaaagttc tataagtctt atatctacat tttattcttt aataggtcta
     4561 gctatatcta tctctagtct aataccatca atggataaat tatcctttat gactaatctt
     4621 tcaaagttca caccatttta ttggactata gatgctatta aaaacaatgg tagtatttta
     4681 ataaatataa tagctctaat attaattgga attatatttg taactgcagg aagtcttaaa
     4741 ctaagagatt ttgctaaaaa ctaataatta ttaaatataa ttagtatcta tttttatata
     4801 attgtaaaag aatatttcaa catattaaat tattttatct tatatttttc tctttagttt
     4861 cacacatcac caagtctaaa ctctatatta tatattaata tttttgactt gtcaagttat
     4921 ttatgttata atattaatca ttagataatt aaagcattgt acctaagttg caatatctaa
     4981 aattcaattt ggaggtataa ctagcatatg tataaaattg gtgaaagtgt aatgtatcca
     5041 aaagagggag cctgctctgt aaatgatatt gtaacaaaga aaataaatca tgagatgcaa
     5101 aaatactatg aattaagtgt catttttaat agtaacctta aaatttcgat tccagttcta
     5161 aatgcagata gaattggcat acgtccagtt atggatggaa atgatgttga taattttatc
     5221 caatctatta ataaaacaga tggtgtatgg atttttgata gaaaagaaag acttaaactg
     5281 taccaagata aatttcattc aggagatgtt tttgaaattg taaaattaat caaaatgctt
     5341 atgatacaag acagttcaaa acaattgtgt agcacagata aggagttttt aaataaagct
     5401 caaaaatttg ctttatcaga attagctgct gctcaatgta aatcctatac aatggtatta
     5461 gaggaaatga aaaaacatat attaaattca aagaatacaa attaaaccag ttttttaaac
     5521 tggttttatt ttttatacta atcatccatg ctactttagc ttattttaaa ctgcctaatc
     5581 taatattaat aaagttatct caattagata gatgtatttt attcaaataa aaaaaactaa
     5641 gagtaaatca tattactgat ttactcttag ctaataaaat ttaatcttct tcaagttctt
     5701 ttttctcttg taataataca cttaattcat ttgcttctaa aacttgtgcc tctgcaatat
     5761 caagtaactc ttgttgccat tccacacatt tatctttgta atcaaaaggt agcgttctgt
     5821 caactatgta cttatcaata cagtcctccg cacttttaat tatttctttt ctttcatcaa
     5881 taaatttttc taattctact aaggcttctt cataagcttt tctactcata taaattcccc
     5941 ctttgtacaa ttattctaca ttaaaatgat ttttcctctt ttttgtataa aattttattt
     6001 gtatataact tttccatcaa tatcttttat agtttgacat ccagttaata tcatagaact
     6061 acttagctct gattgaagtt tatttacata agtttctaca ccatcagttg caccaccaaa
     6121 agaagcagtc acaaaaggtc ttcctattaa tactgcatct gcacctaaac ctatcatttt
     6181 tagtacgtct aaaccagttc tcactccacc atctgcaagt attgtaactt tacctttaac
     6241 agcatcagca atttttggta aaacttcaca tgctcctggt gtacagtcaa gcactcttcc
     6301 tccatgattt gatacgacta ttgcatccac acctgcttct actgccatta aagcatcttc
     6361 aacagtcatt atacctttca atataaatgg tagtttagta gactttacta actcctttat
     6421 ttgtttaaca tttttaggaa gtactggttt tccatgtaat gataaagtta ctagaccaca
     6481 ggcatctata tcaactccaa ctgcaaatgc accagcttct tcagctaatc ttatcttttt
     6541 tataatattt tcattatccc aaggtttaat aaatacaatt ccagctccat tatacttttt
     6601 gactacatct aaattttcca ttaaaaaagc atctactgcc gtatcaccaa ccattgcata
     6661 aataccacta ttaacacatc ctgcaactac tggttctatg taatctcttt cattaatttt
     6721 tccacccata tttaaagtag ttcctgttac tggagctgca aatataggtg cactcatttt
     6781 ctttccaaac atctctatag atgtatcagg atttgataca tcatgtatta ctctcatatt
     6841 tacttttact ttttctaaac tcttgctatt ttctataaaa gaagaaccac ttcctttacc
     6901 tcccatgcca ggcacttcac ctgcacaagc aaccccatta caaaccttac aaactttaca
     6961 acttccattt aaattttctc tagcactttt taataattca ttatattcca tctttaatcc
     7021 cccattaact atagtaattt atattatata cattatatca tattatattt acagtatatt
     7081 tttcaaaaaa ctttttctgt ggataggagt tatgccatat ttttcaatag cttcatagtg
     7141 ttctttagtt ccatatcctt tgtgagattt aaacccatat tctgggtata ctctatcata
     7201 ttggtacatt atactatccc tagtaacttt agcaagtata cttgctgctg ctattgatat
     7261 agattttgaa tctcctttca caattggatt ttgagatatg tcaatacctg gtattgttgc
     7321 tgcatcaact aataaataat ccggtgcttt ttttaaacaa tttatagcct ttttcattgc
     7381 catatatgta gcatttaata tattaaattc atctatttct tcattgttta ctatacctat
     7441 tccataatct agtgcttctt cttttattac ttcaaaaagt tcatctcttt ttgcctcact
     7501 cagtttctta gagtcattta caccttctat tttggtatct tttttaaata ctacaacaga
     7561 agctacaaca ggtccagcaa gtggtcctct tcccgcctca tcaattcctc caatatataa
     7621 ataaccttta tcatagcctt cgttctcaaa tatatttata gtttccaatc tttcttcttc
     7681 ttttctaata ttatccagct tttttgccag ttttatagct agaccttgta cagattttct
     7741 ttcatcaact cttaacagct ctatatattc catatatttt tctacttcta aagtctctat
     7801 tatttcttta atctccctca cacttttatc ctgcatacca ttccctcatc tttcgttttt
     7861 aaaaatttaa tttttaaata tattaaaatt ttttaattag agtttaattg catccactat
     7921 agccacattt taaacatata ctacatccac ctgtatgtgc aattccttca cttccacact
     7981 cagggcacac aaatttttca tctccttcaa aatttttatt tattatttct ttggcttttt
     8041 catctttatt atctttttct tttttattat ttttataata ttctttttta ctattttctt
     8101 ttttatcttc tttattattt tcttcctttt tttcatttat taaaattcca ctccttttac
     8161 atccatctct aaatattgtt actcctttaa gaccagcctc ccatgcctta atatatatat
     8221 tgaaaacatc ttcaactgta gtttcaaaag gtatatttat tgtagatgat atagaagcat
     8281 caatatactt ttgccatact ttttgcatgt ttattctttc ttcatatcct atattcatag
     8341 cagtaacaaa aaaatctggt aaacattctt catctgtaat acctttcttg tccatatact
     8401 ctttaacaat tggagtatat actttataat acacatcctc atcatgcaag ctttcagttt
     8461 ttcttatata tgaaagatta aatataggtt ctattccacc actgatacct atcattgtag
     8521 atatggaacc tgtaggaggt atggttaaaa gttgtgaatt tctaagccca tactttttaa
     8581 ccatttcctt agtgtcttca tttagatttt ctataaaaaa cttagattta gaaatatttt
     8641 catatttata ctcactaaat gttccatatt ctttagcaat taatgctgat tgcttcacag
     8701 cttcatttaa catagtctta ccaattaaat cacatagttc taaagattca tcagaaccat
     8761 atcttattcc catttttata agcatatcag ctatacccat tacacctaaa cctatctgtc
     8821 tatacttatg tacactatct ctttgttctt gtaatggatg caagttcaat ccctcatcta
     8881 agacatcatt taaagctact acagcttctc taacgcaaga ttttaattta ttaatatcaa
     8941 aagtagcatg ttcaccaaat ggttgtatta taaactctga caagtttatt gagccaagta
     9001 gacaacttcc tccacttgga agtggttctt ctgcacaagg atttactcct gaaaagcaaa
     9061 agttcttatc ttcactcata aaattatatg tttttatttt atcccaaaac aatatacctg
     9121 gttctgcata atcccagtta tttttcacaa atttattaaa taaatcataa gcatttacta
     9181 tcttaactat tttttctcca gtagtttcaa cttcaaaagt acaattatat aacttttttt
     9241 ctcttacagc ttgcataaat tcatctgtta ctcttacaga aatatttgct ttagtaacct
     9301 tctccaaatc tgtttttata tctataaagt cttctatctc tggatgattt acatccatag
     9361 aaatcataag agctcctctt ctccctcttt gaccaattaa gccagttgtc atactgtaaa
     9421 gttccataaa agagactgct ccagtcgtat gttttgcagc attgttaact ttcgctcctt
     9481 ttggtcttag ctttgaaata tcaattccaa ctcctccacc ataactaaaa gttctagcaa
     9541 gtttttttgc agtatcaaat atatcttcta tgctatcttt tggtggttct attacataac
     9601 aattagaata tgttactttt tttccctctt tctgtaatcc tctatttgct aaaattcttc
     9661 cagcaaatag aaattttttt tgtcttatta gttttgatat tcttgaacta ttatttgaaa
     9721 ttctctttat ccattcatca aaactctcac cattatgtct atacttgttc ttccatattg
     9781 atttttgtaa ttcatcaaac tcccaagctt tagtccttat ttcagagcgt ctttctctat
     9841 ataatatgta gtgttttgct atatcttttc tatttgattc cataagtagt acttcaacta
     9901 tatcttgtat ttcttctacg gataagcatt tttccataga attatagtca ttagctattt
     9961 ttcttgatat accatatgcc aactctctat ctactccacc ttcactattt atactagctt
    10021 tatagacagc atcttcaatc ttacttttac taaaaagttc ttttgtacca tctcttttta
    10081 ttatgtattt catttttaaa cctccaataa attagtatta aattaataca cacattaact
    10141 ataactctaa acaaactttt tatatatttg tatattttca tattaattat ctaacataat
    10201 atatattata ct
//
LOCUS       DAMJZE010000147        10174 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJZE010000147 DAMJZE010000000
VERSION     DAMJZE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.151"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            317..1477
                     /locus_tag="PC541_002963"
     CDS             317..1477
                     /locus_tag="PC541_002963"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2840407.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1479..2609
                     /gene="glgD"
                     /locus_tag="PC541_002964"
     CDS             1479..2609
                     /gene="glgD"
                     /locus_tag="PC541_002964"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2840408.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2638..4080
                     /gene="glgA"
                     /locus_tag="PC541_002965"
     CDS             2638..4080
                     /gene="glgA"
                     /locus_tag="PC541_002965"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2840409.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4094..6514
                     /locus_tag="PC541_002966"
     CDS             4094..6514
                     /locus_tag="PC541_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2840410.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6519..8384
                     /locus_tag="PC541_002967"
     CDS             6519..8384
                     /locus_tag="PC541_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2840411.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8629..9282
                     /locus_tag="PC541_002968"
     CDS             8629..9282
                     /locus_tag="PC541_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2840412.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9378..9977
                     /locus_tag="PC541_002969"
     CDS             9378..9977
                     /locus_tag="PC541_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HDF2840413.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         4088 a          992 c         1860 g         3234 t
ORIGIN      
        1 attaaaaaat aagcatgttc atatgacaca aaggatttat aaaaaatgtt tttatattta
       61 taaaataaaa tttgtattat tttttaaccg ataattgaat attaataaat actatagtgc
      121 ttatggaaaa taatgtatac gtcggtaagt ccattatggg gtatacatta tatcaattgt
      181 atagttgata ttattttatt tttattaagg tattatttaa gcaacattta tacacgacat
      241 ttattgactg ataaagttta taaaagtaaa aatgcaatta taaatattaa ttactagatg
      301 aggggagtgt cattaaatga aaaaagagat gttagctatg attttggcag gagggcaagg
      361 ttcaagatta ggtgttttta caaaaagaat tgccaaacca gctgtatcat ttggaggtaa
      421 gtatagaatt atagattttg tgttaagcaa ttgttcaaac tctggtattg atactgtagg
      481 tgttttaact caatatagac cgttgatatt aaattcacat ataggcatgg gaagtcattg
      541 ggatttggac agaataaatg gtggtgtcta tgttttacaa ccatttatga atgaaaaaga
      601 aggaaattgg tataatggta cagcacatgc gatatatcaa aatatggact ttgtagatac
      661 ctataatcca gaatatgtat tgatattatc tggtgaccat atatacaaga tggattatag
      721 caaaatgtta aaatttcata aggaaaaagg ctctaaagct acaatagcag tgattgaagt
      781 tccttgggat gaagcttcaa gatttgggat aatgaataca aatgaagatt caagtatcta
      841 tgaatttgaa gaaaaaccaa gtgaacctaa gagcaatttg gcatctatgg gagtatacat
      901 atttgattgg aaaatgttga gaaattattt taaggaagca gaaaaaaatc cagaaataaa
      961 ttatgatgat tttggaaaaa atttaatacc taaaatgtta gaagataatg ttggaatgta
     1021 tgcttatcca tttaaaggat actggagaga tgtaggaact atacaaagtc tatgggatgc
     1081 taatatggat ataataaaat ctccagaaac acttgattta gcagacccta agtggaagat
     1141 atatacaaat actatggcaa tgcctccaca atatataggt aaaaatgcaa atgttcatag
     1201 atcaatgata gctgatggtt gtagaatttt aggtgaagta ggtaactctg tattatcaca
     1261 tggagttgtt gttggtaaag gaagtaaagt aattgattca gtaataatgc caaatgttgt
     1321 aataggtgaa aatgtaacta tagaaaaagc tatgataggt gagtgtgcaa ctataaatga
     1381 taatgttcag ataaagaatg taaataatga aataaatgta gtttctgagt atgaaaatat
     1441 cgaacctaga tgcgtattaa tagaaggagg tctttaagat gagaaatgaa tgtttaggga
     1501 taataaattt aaacaaaaaa ggagacccag caataaacaa acttaactat ggaagaccaa
     1561 tagcatctac accaatagct ggaagatata gaataataga ctttgcttta tctaatatga
     1621 taaattcagg aatcactaaa gttggaatat tcgcaaaaga aaaatacaga tcattaacag
     1681 accatatagg tagtggtaaa gattgggatt taagtagaaa aaaaggtggc ctatcaattt
     1741 ttagtccaga aaacactaag tatagaaata cttactcaca tagagaaggt gatatatata
     1801 ctatcttagc taatctagat tatatagaaa agagtgaaga agaatatatt ttaatagctc
     1861 caagctatat gctatgcaat ttaaattatt ctcaagcatt ggaataccat aaaaaatctc
     1921 ataatgatat aactataata tataaaaatg taaataatgc aaataaagat tttgctggta
     1981 acttaactct aaatttagat agtaacaata gggtaataaa tgtaggaaat aatttaggta
     2041 agttccctag agcaaacata tgtatggaga catatattat gaagcgtgaa gattttgttg
     2101 aatgtatata taacattgta aataaaggca attattgtta tttagaagaa tttattattg
     2161 aagaagcaga aaacatgaaa ataggagcct atgaatatac aggatatcta aaatgtgtaa
     2221 attctgttga atcatatttt gaaatgagca aagatttatt aaatttagaa gttgcagatg
     2281 aattattata ttctgaaaga aagatattta caaaagaaaa gaatgaatca cctacaattt
     2341 atacagatag tgctaaagta gaaaatagct ttatagcatc tggttgttta atagaaggta
     2401 ctgtaaaaga tagtatcata tttagaaagg taaatgttga aaaaggaaca gttatagaga
     2461 actcaatagt aatgcaaaat tgtgtaataa agtcaaatgc aaaactatat aatgcaatat
     2521 tagataaaaa tacaagtgta tcaaatggga aagaattaag aggagatgaa aaatatcctc
     2581 ttgtagtgga aaagaataca aatatataaa taaatttttt agaaggggga atgactaatg
     2641 aaggtttttt atgtaacagc agagtgttgg ccctttgcaa aaactggagg attaggagat
     2701 gtttcttatg cattgcctaa agaattaaaa aaagaaggtg tagatgtaag ggtaataatg
     2761 cccaaatatt caactatacc aagttattta aaagatcaac taaaagaaat agctgtgttt
     2821 agtgtacgtg ttggatggag aaatcaatac tgtggtcttt tagaaatgga attggatggt
     2881 gtaaaatttt actttataga taatgaattt tattttagaa gagaagatga aagaaagagt
     2941 atctatggat atggagatga tgcagaaaga tatactttct ttacagatgc agttttagaa
     3001 gctataagta gaattgattt ttatccagat gtaatacata taaatgattg gcatacaggg
     3061 atgttaccat taattctaaa agaaagatat gcaacccttg aggggtataa aaatataaaa
     3121 actatgtata ctatacataa tctccaatat caaggtgtat ttgataagca tgttttatat
     3181 gatatattgg acttaccaca aaaatacttt gataatggag atatagagta ttatggctca
     3241 ataaatttta tgaaagctgg tataaatttt gcagataaaa ttataactgt tagtcctact
     3301 tatgcaaatg aaatacaaac atctttttat ggcgaacaat tagatggatt acttagaaaa
     3361 gagtcaggaa aattaaaagg tatattaaat ggtatagatt atgatttaaa tgaccctgca
     3421 aaagataaag atatatttgt tcattatgat gtagactcaa ttgacaaaaa agtagaaaat
     3481 aaattgagat tacaagatat attgggacta aaaaaagatt catctattcc gttgataggt
     3541 atagtttcga gattagtttc tcaaaaagga tttgatttaa tagcatacat gatgccagaa
     3601 cttatgagag aagatttaca gattgtcgtt ttaggaactg gagaacatca atatcaatca
     3661 atgttcaact attacgattc taatttttca gataaagtat cagctagaat tacttttaat
     3721 gcatcattgg ctcaacagat atatgctgca agtgatatgt tcttaatgcc atcattattt
     3781 gagccttgtg gtataggtca aatgttagct atgagatatg gttctttacc aatagttaga
     3841 gagactggag ggcttagaga tacagtaaca ccttataata aatttactgg agaaggcaat
     3901 ggatttagtt ttaaaaatta caatgctcat gagatgtttt tctgtttgaa aaatgcaatc
     3961 aaagtattta aggacaaaga aaaatggatt aaattagttg aaaatgcaat gaaaacagat
     4021 aatagttgga aaaaatcagc taaggagtat atagaaacat atagagacat atgtgattaa
     4081 aggagtggtt tttttggtaa caataagcaa aaagaaattc aagcaaaaat ttgaagtaaa
     4141 aatgtatagc ttatatgcac aatctatcaa ggaagctact gatgaacaat tgttaaatgt
     4201 attatgtagt ctattgaaag atgagatagc aaaaaaatgg gttgctacaa agttagataa
     4261 gaaaaaagaa gtttattatt ttagtttaga atttttaata gggagacagt taaaatctaa
     4321 tttattaaat ttaaatattg aagaagaagt tagagaaggt ttatctgaat taggaatcaa
     4381 cttggatgat ttaattgaag ctgaagtaga cccagcctta ggaaatggtg gacttggtag
     4441 attagcagct tgctttttag attctatggc atctttgaac ataagtggtc aaggatatgg
     4501 aataaggtat aaatatggtc tgtttgaaca aaaatttgta aatggttatc aggtagaagt
     4561 gcctgataac tggcttacag aaggacgata tgcatgggaa actgtaagac caaatgaagc
     4621 aacaatggtg aagtttggtg gtgaggtaga attaatcaag gaaggtagtc atttaaaagt
     4681 aattcataaa aactaccttc ctgtaatggc aatgccttat gatataccaa taatcggata
     4741 ccaaaaccaa tgtataaata ctttaagatt gtttaagagt gaaataccaa agagagattt
     4801 tggtgaactt acctcaaatg ctctaaatta ttctggtagc tatgaagagg ctttaaaaca
     4861 taagtattat actgaggaaa tatcacaggt attatatcct gatgattcaa actatgcagg
     4921 taagttgttg aggttgaaac aagaatactt ttttgtaagt gcagggatac aagatataat
     4981 aagaaaatat aaaaagaaca aattaaatat taataattta ttcgataagg tagcaattca
     5041 tataaatgat acacatccta ccttatgcat accagaactt atgagagttt tgcttgatga
     5101 agaaaattta tcttgggatg aagcatggga aataactaaa aaaacagtgt cctatactaa
     5161 tcatactata atgtcagaag caatggaaaa atggcctgta agtatgatga aagaactatt
     5221 accaagaatt tatatgataa tagaagaaat aaatagaaga tatgtagagg aattaaataa
     5281 taaaggatat gaccaagata gaataaaaag aatgagtata atagactgtg acaatatcaa
     5341 tatggcaaac ttatgtattg tcacaagtca tagtgtaaat ggagttgcta agcttcatac
     5401 tcatattctt gaaacagagg tattaaaaga tttctatcaa gatgaaccaa ataaatttaa
     5461 taataagact aatggaattg cacataggcg atggcttata agttcaaatc ctcaacttag
     5521 caatttaatt acagatttaa ttggtgattc atggaagaaa gatacattac aattaaaaaa
     5581 tattgaaaaa tttaaaaatg attcctctgt tttacaaaga cttgatgata taaaatacaa
     5641 taataaagca aatctagcaa agtttataaa agataaatac gatttaaatg tagacccaag
     5701 ttcgatattt gatgttcaag ttaagagatt gcatgcgtat aaaagacagt tactgaatat
     5761 atttaatgta cttcatatgt atcatgaatt acttgataat cctaatttga atcttgaccc
     5821 aagaactttt atatttggcg caaaagcagc accaggatat tatttggcta aatgtataat
     5881 taaatttata aattctgttg caagtactat aaataatgat gttagagtaa aggacaagtt
     5941 aaaagtagta tttttagaga attatggagt atcattagca gaaataataa taccagcagc
     6001 aaatgttagt gagcaaattt caactacaac taaggaagca tctggaacaa gtaatatgaa
     6061 gtttatgatg aatggtgcta taactttggc tacacttgac ggtgcaaatg tagagatatg
     6121 tgaacaggtt ggtaaagaaa atatgttttt atttggactt agtgcagaac aagtactaaa
     6181 ttataataaa tatggtggat attcttcact tgacctatat cattctaata tggatataaa
     6241 aagggtagta gatgatttaa taaatggatt tattcctaat cttggcgaag aaggaagaag
     6301 tatttataat tcactcacta cttacaatga tgagtatttt gtattgagag attttgaaaa
     6361 ttatggtcaa gctcaagcag atataaacag gttatataga gataaagaaa aatggaataa
     6421 aatgtcttta gttaatatag ctaattctgg atttttttct tctgatagaa caatttctga
     6481 gtatgcaaaa gacatttggt ttaaaagggt gtgattaaat gcaaagtgta atagaatata
     6541 actcttggga taaaaatttt aaggctccgt ttggagcttt aaaatttgat gaggaattga
     6601 ccatttgtgt taaagtaaat gagggataca atatcaagtc tatttcccta gaaataaata
     6661 gagaagaaga aatgagaaca ataactttaa atgaagaatt agataatgat aaacttggaa
     6721 agtgcttttg tggtaagatt gaaaagtttg atggtacagg tgtttatttt tattacttta
     6781 aagttgatgt tgaaatggat ggtcagataa agactttatt ttatggaaaa aacagagata
     6841 atggatattc ttgtgaatat aattatagtg atattaataa atatcaaatc actgtttaca
     6901 aagattttaa agtaccaaca tggtacaaag aaggaatact ttatcatata tttgtagata
     6961 gattcaataa tggaaatcgt agtggtaagg ttgataatcc taaaaaaaat agtttcatat
     7021 atggaaattg ggaagataca cctatgtaca taaaggatag tcaaggggat gttataagat
     7081 gggatttcca tggtggaaat ttaagaggga taatcaataa acttggatat ttaaaaaaat
     7141 taggtgtaag tatactatat ctaagcccta tatttgaagc ctctagtaat cataagtatg
     7201 atacaggtga ctataaaaaa attgacccaa tgtttgggga tgaagataca tttaaagaac
     7261 ttattgataa agcaaaagaa aaaggtatat caatagtatt agatggagtt tttagtcata
     7321 ctggagcaga tagtaaatat tttaatatgt atggaaacta taatagttta ggggcatatc
     7381 aatctaaaga atctccatat tattcatggt atatgtttga agaatttcct caaaaatata
     7441 aaagttggtg ggatgttaag acattgccaa atataaatga attagaacat agctatatgg
     7501 attatataat atatgacaat gatagcgtta taaataaatg ggtaaatatg ggtattaagg
     7561 gctggagact agatgttgca gacgaattac caactaagtt tataagagag ttaaaaaagg
     7621 aattaaaaaa ggcagatgat gactcaattc ttataggtga agtatgggaa gatgcatcaa
     7681 ataagattag ctatggtcag agaagaagtt atttattagg tgaagaatta gattctgtta
     7741 tgggttatcc ttttagaaat aacatgtttt ctttcttaaa aggagaaatt aattcatatg
     7801 aactttgtaa tagatatatg cagattaaag aaaattatcc aaaagaatca tttaggtcta
     7861 atttaaattt aattggaaca catgatgtta caagagctaa aacggaatta aatgatgatg
     7921 tagatttagt gaagcttgct gtagctatac agatgacatt tgaaggagta ccatatatat
     7981 attatggaga tgaagcaggt ctttgtggag gtgttgaccc agataataga agaacttatc
     8041 catggaaaaa tgaagacgaa gatatgctta atttttatag agatgttata agaattagaa
     8101 ataagaataa acttttaagt tctggaaata cagaatttat atatactaat aatgattctg
     8161 tattttcttt tattagagtt aatgaaaata atgatagaat gttaatttta gtaaatagaa
     8221 gtgaaaatgt ggaaagtata tcattatgta tagagagtag ttttattgag gaaataccta
     8281 taaagtatag tcttaaaaat gcaaatagta ctatacaaat tgaaaataat gagttgaaag
     8341 tagatttaga ctctaaatca tttagaattt ttagagtaaa ttaaatatct ttttttagaa
     8401 aaataaatag aaaataaggt gaatgtggaa agagttttgt tttatcaaaa ctttttctac
     8461 tttttttgta taaattatca taagaatttt agtatttcaa aatattacaa agaagtataa
     8521 aaaatatcat aattgtgata ttatataaat tagtaagata aaactgaact ctctaccaaa
     8581 gttgtaggga gatttaactt ttataatctt aatatggagg aacgtcttat gaaggtagat
     8641 ccacttacaa ctcaagtata tgactatata tcaaagaaaa tccaaaatgg agaatatgaa
     8701 gctaatcaga gaattactga atctgagatt tgtaaatcta tcggagttag tagaacacca
     8761 gcgagagaag cattaactag gttagcaggt gaaaatttac tagaaaaaat acctaataag
     8821 ggatttgtag taaaagaatt tcaggaaaag gagaaattag acacctattc cgtaattgga
     8881 gtattggatg cgttagccgg ttcttcagca ttacaaaatt taactgaatc agacctagta
     8941 aaaatggaag aattaacaga aatgatggcg gtctctataa agtataaaaa ttacaatagc
     9001 tatttaaaat taagtaatga atttcacgat atatacataa ctaaatcaga taatcaggtg
     9061 ttagtaaatc tattgaactc tttaagatat aattttatgt caaagtcata tactagtgat
     9121 aatgaagatg agttatataa aatgttgaca tacagcaata atcaacatat tgaagtagta
     9181 aaatctatga gggagaatga tttagaaaat gttgaaagaa ttttgagaga acattggaaa
     9241 acgataccta tggaagagat gaaaaaagag aagcttttat aaagtttctc tttatttttt
     9301 ataccatttt atcactattt ttttgaatta ttaagtagaa aataataatt aatttaaact
     9361 tgaggaggaa aactaatatg tatccaaata aagaaattgc aatagaagag ttagaagtgg
     9421 ctggcaaaat gaatcccggt ccatggataa aacattctta caatgttgct aatgtagcta
     9481 aattgattgc agagcaatgt gaaaatttga atagtgaaaa ggcctttgta tgtggattat
     9541 tacatgacat tggaagaaga actggtattt cttctgtaag gcatattatt gatggatatg
     9601 attatgttat ttctaaggga tgggatgaag tggcgagggt atgtttgaca cattcttttc
     9661 ctgttaagga tatagaagct gatattggaa ataaagatat tagtaaagaa cagtatgatt
     9721 ttatcaatca ctatttaaac aaccttgaat ataacgatta tgataagttg attattcttt
     9781 gtgatgcatt agcagatgca aatgggtttt gtattttaga aaaaagattt gttgatacaa
     9841 cacgaagata tggaatatat ccattttcag ttgaacgatg gaataagact tatgagtata
     9901 aagaatattt cgagaaaata atcaacaagt ctatatatac attgttaccc aatattgaaa
     9961 aatctatata ttattaaatc ctaagttaat aatttaatat ccagtatcac cagcagttgg
    10021 tcattatgat tgactgcttt tattatgctt atattaaaaa aatacactat ggtttgtgta
    10081 tatcaaagtt atatggaatg aaaagagaat ttattttatc aattaaaaaa tttaataaat
    10141 tttatattaa aattaaaata caaaaatagg aaaa
//
LOCUS       DAMJZE010000148        10076 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJZE010000148 DAMJZE010000000
VERSION     DAMJZE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.152"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(453..1610)
                     /locus_tag="PC541_002970"
     CDS             complement(453..1610)
                     /locus_tag="PC541_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2840414.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1764..2333
                     /locus_tag="PC541_002971"
     CDS             1764..2333
                     /locus_tag="PC541_002971"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2840415.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(2515..2931)
                     /locus_tag="PC541_002972"
     CDS             complement(2515..2931)
                     /locus_tag="PC541_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840416.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3157..4251)
                     /locus_tag="PC541_002973"
     CDS             complement(3157..4251)
                     /locus_tag="PC541_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840417.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(4674..5615)
                     /locus_tag="PC541_002974"
     CDS             complement(4674..5615)
                     /locus_tag="PC541_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2840418.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            complement(5608..6435)
                     /locus_tag="PC541_002975"
     CDS             complement(5608..6435)
                     /locus_tag="PC541_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2840419.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(6606..6989)
                     /locus_tag="PC541_002976"
     CDS             complement(6606..6989)
                     /locus_tag="PC541_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2840420.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(6958..7245)
                     /locus_tag="PC541_002977"
     CDS             complement(6958..7245)
                     /locus_tag="PC541_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2840421.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(7278..8435)
                     /gene="alr"
                     /locus_tag="PC541_002978"
     CDS             complement(7278..8435)
                     /gene="alr"
                     /locus_tag="PC541_002978"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2840422.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(8450..9040)
                     /locus_tag="PC541_002979"
     CDS             complement(8450..9040)
                     /locus_tag="PC541_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840423.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(9061..9522)
                     /locus_tag="PC541_002980"
     CDS             complement(9061..9522)
                     /locus_tag="PC541_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2840424.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(9537..9917)
                     /locus_tag="PC541_002981"
     CDS             complement(9537..9917)
                     /locus_tag="PC541_002981"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2840425.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         3240 a         1862 c         1059 g         3915 t
ORIGIN      
        1 aacctatttt tatatcgata agtatgaaat tgtttaacca agtcaaaatg atatataaat
       61 ttatatttaa atgtgtttag agtttaattt attttcaatg tttaaaaaag ttattctatg
      121 catctaattt aattagtaaa ctgaggttct agttagataa attatattat aatgaatata
      181 tagtatgcag tttacgatat tttaaaagta aatgatataa tatttggaac aactgggttg
      241 caatgtgttt ttaaaaaata tcaaaataag gctatgattt ttaaaaccat agccctgtct
      301 gtaaaaattt aattatcact taaaaaataa atttttattt aaagaataca cataaatatt
      361 tccagactcc ctcatgaact aaaaagttca ttttgagaca tctcttttat aatgttcttt
      421 attttatagt ataagtttat aaaaattaaa tcctatttat caatctgttc tctaagccac
      481 ttagttgcta catctaactg tttattttca tctttttctt caatagtagg ctttatacct
      541 ttttcattga tataatctcc attaggtgta aaatattgag cagtagttaa cttgtatcct
      601 gttccatcct tcaatcttac aactgattgt accaaccctt taccaaaagt tgtagtccct
      661 acagaaatac cttctttatt atcaacgatt gcagctgtta atatttcagc agcagaagca
      721 ctttctccat tagttaacac tactattgga agtcccaatt tttctttatc agatttcaag
      781 tactcagtat ttcccttatt atcttttgta tatactatag ttccttcacc aattaactca
      841 tcagcaactt ctttacatat atctagcatt ccaccaggat tatttctaag gtctattact
      901 aatcccttga tattttttgc ttttaattct tttaaagctt tatcaaagtc agtatatgta
      961 cttgatataa attgggtaat ttctatatat cctaaatcat tatcaattac tttactttct
     1021 atactcttct caattatctg gtctgttttt actttaaagt tcaattgttt atcttctctt
     1081 aatatagtca gttcaactgt tttacctttt ttacccttca tcatacgaac agcttcatct
     1141 gaatttttat atgagacaga ttttccattg acttttacta atttatcccc tgattttaca
     1201 cctgctttct ctgctggaga atctttcatg ggaactacta ctaagccacc atcttttgat
     1261 gcccctatat acatacctac accaacataa gaaccttcac tatcattaat tagattctcc
     1321 atttcatctt tagtataata accagaatac ttatcatttg ttcctagaaa tagacctttt
     1381 gctgctccat ttaccaaatc agtatctgat acttttttat aaaaatcttg ttttataata
     1441 gattctagtc ctaacaactt atcatatttt ttatagtctt catagagttc tttcgatatt
     1501 acaactttat tccccaatgt caactgaaat gctgatgtaa ccatagctgt tataattact
     1561 aaaatcaccc ctaaaaaaat agctttcttc tttgaaatca caagatacat ctccttttac
     1621 ataaactatt attattatat gactagctgt tatttttatt acatttaccc aaatatattg
     1681 ctttattaca ataaattata ctaaaattta tattaaatac aattgctgta cacattataa
     1741 catattaagg gaggaattat tttatgaaaa aagaatttag aaaaaaagtt attgaatcaa
     1801 gaaaaaataa gagtgcaaac ttcgtactta gtaattcaaa tttaatcact gaaaaattac
     1861 ttcaaatgga agatataaga aatgcaacta atataatgct ttatctaaat tttaacaatg
     1921 aagttcaaac agataattta atcaaaaaac ttttgtctct aaagaaaata gtatcaagtc
     1981 ccatcacaat aaaagaaaat catacattaa tccctactca aataacagat ttaaaagagg
     2041 gattgagaat tggagcatat ggaataagag aacctaacga aaaatcacct acaattgata
     2101 ttaaggcctt agatgttgta attgtaccag ctgtagcata tgatatacat tgctatagat
     2161 taggatatgg tggaggattt tatgatagat ttttagaaaa cttaagaaaa gatgctataa
     2221 ctatcggcat agcatttgac ttccagatat tcgattctat tccaaaagaa actcatgatg
     2281 cacaattaga ctacatagtt actgaaacta gaattatcac tccaaataat tagaatatta
     2341 agtatttcca gaccccattt aatatagtaa taaatactac tgttaatatc ttactttata
     2401 agcattttaa gtcatggctt ttacaacaat aagaaccata tacaatttac ttacattaat
     2461 aaaaaccatg actttattgt acaatatgca gttatttaat tataaaatac cttcttattt
     2521 cgtcgcattt aattctattc tatctggaag ttcactcata tttgtttcaa caactatgta
     2581 tggaaaagaa tcttccttat ctttatactt tatcttgact ctcaaaacat cctttccttc
     2641 ctcaaccaca ctttctattt tgtccatttc tataccatat ttattatttc ctctagtcac
     2701 tataatgtat attttgttat cgattttagc cgctaatgct ctttcttcat ttgtatactt
     2761 atccatcaca tctaatatct tttctggtat tgaatttctt tgtaatactg tataatctat
     2821 gggtttagat ttctctacaa acatacttgg tatgaatata aatcccccta taacgaatac
     2881 aagagctata actccataaa ttataccttt aatattaaac tttacattca atcttatcac
     2941 tcctcccata tattctaaat atatgattta taaacttatc ttagactcta aattattgat
     3001 tagtatatat ttatacataa ttataaatat atgtaatgta actaaaaaac atgttttttc
     3061 tatttcataa taaacgagct aaaaaataaa aagagctata tgaaaacatt ttcattgttt
     3121 tcatatagct cttgagttta caatattaaa aaatacttat ttgtcactga ttccttcttg
     3181 atatccagat gtttttgtaa tctgtctata aagaacacaa accagtatta tgacgcccat
     3241 atatccagta tatggatata aaataccaac cagcttatca aatggtagga agccacctat
     3301 aagacctaat atagctgcta ttaaagttat taatttaaac ttaggatgtt tatcatctac
     3361 aaatctatta gttactgacc ataataaagg tacagctgtt gtatatatac ctaatataag
     3421 aattattgag aataaaactc caattactgg tgaaatctta tctgctaaga ataatgatgg
     3481 tattgctttt gaatataagt ttcctatatc agaaagaata gctaagtgca tcattatagc
     3541 tgcagccata agtgctactc cacctaatat accaccacaa atagcttctt ttttgttagc
     3601 agcacttgct cctaatccag ttaagaatgc cataacaaca attgtgttat aagtagtata
     3661 tattacacca gatagatagg catttggaac tggtttagct atctttacag tgctaagaac
     3721 ttgtgcagct tctcctatat tacctatgtt tttagctaaa cttataccac caactaaaag
     3781 tgtaaagatt attattatag gccctattcc acctagtatc tttgataatt tatcaagacc
     3841 tagtgaaaca gttaataatg ctactactgc catacctact ctacctacaa atggatttaa
     3901 tccatagtat tcagttaaag ttgctcctgc tcctgaaatc attactacga aaacaccaaa
     3961 taagaataaa ggtccaaacc attcaaagaa tattcctaaa tacttaccac aataaacacg
     4021 ataaattttg ataggttctt taagttgaag ctctttacct tttgacataa ctgaagcacc
     4081 catccatgag aataatacca ttgaaattat cccaccaata atacctgcaa aaccataaaa
     4141 agagaagaat tgcattattt cctgtcctgt tgcaaatcca gaaccgatta ctgttgctac
     4201 atacgcacct gcgaaactga tgactgtttt aacatttact ttttctgaca tatctcagtt
     4261 acctccccat gtgaaatttt ttcttctgat taaacaattc gataaactac ctacaatttc
     4321 ctttaacata aaatacatga taactgtttt atagcttttt agtataataa cttaaaactt
     4381 ataatctgat tttataccct actcctcctc tcataatttt tttagattct tttatctaag
     4441 tagtcattat atataaaatt agtttctcta taaattatat attagtcata tttttctgaa
     4501 tagtcaatat atatttgaat attttctcta tttttgtaaa aatttgaata ttttattaca
     4561 tttttgatat attgatatca tataaaaaaa agagagatat aaattaatat aacactttat
     4621 acctctcttt taaatatatt atattattaa caaacatgaa taatttatat ctcttacatt
     4681 ctttttgcaa taataacttc tttaacagtc tttattatgt catcacaagt taattcatat
     4741 ttttttaaca attcatttgg agtaccagat tctccaaatg tatctttaat tccaactttc
     4801 tttacaatgg ttggacaaga ctctcctact acctcactta cagcagaacc taatccccca
     4861 attatgctat gttcttctac agttacaata gcattcgttt cttttgctga ttctaatatt
     4921 aaatctttgt ctataggttt tatcgaggac atgtttataa ctctagcact tataccttct
     4981 tcttgcaact tttcttgtgc taatagagct tcatttacca taattccaca agctataatt
     5041 gatacatcct ttccttctct taagactgta ccttttccta tttcaaattt ataatcttca
     5101 tcaaataaaa ctggtactga acttctccct aatctaacat aaacaggtcc attatactta
     5161 gctatttcaa aaataatttt ttctgtttca actccatcag ctggaacaag aactgtcata
     5221 tttggtatag ctctcattat agcaatatct tccacacttt catgagatgc tccatcctct
     5281 ccaacagtaa gacctgcatg agtagcacat atttttacat ttagctttgg ataacaaact
     5341 gaatttctta taatctcaaa agcccttccc gtagcaaaca tagcaaaagt acttgcaaaa
     5401 ggtattttac cagcagttga taatccacaa gcagcgccta ttaaattttg ttcagctata
     5461 cccatattaa aaaatctatc tggaaatgac ttgtaaaaat catgtgtttt tgtagacttt
     5521 gataaatctg catctaatac tacaacattg tcatttattt gacctaattt tactaatgct
     5581 ttaccatatg cttctctcgt cgccatttta ctcattgtca gcacctccta attcaagcaa
     5641 agctttttct agctcctcat cacttggagc tgtcccatgc catcctgctt gattttccat
     5701 aaaagatatt cctttgcctt ttatagtttt tgcaactatc attgtaggtt ttttaactgt
     5761 tgatttagct atatcaagag ccgcaaatat ttggtcaaaa tcatgtccat ctatctcaat
     5821 tacattccaa ccaaaagctt taaatttatc aactattgaa cctaaactca ttacgctttc
     5881 tatatctcca tctatttgaa gtccattatt atctaaaaaa gcaattaaat tatcaagttt
     5941 ataatgtgca gcactcatag cagcttccca aacaattcct tcttgaacct caccatcacc
     6001 aagcaaagta tacacattcc acggagcatt atccaacttt gatgccatag ccataccaca
     6061 tgcaactgaa aaaccttgac ctaaagaacc agtagatatt tccacacctg gcgtgccttt
     6121 catgtctgga tgaccttgta acatccttcc tatcttcctt aatccattca gttcttcctt
     6181 atcaaaatag cctttttcag ccaatgtagc atataatgct ggtgcagcat gtccttttga
     6241 taatacaaat ctatctctat cctccatttt aggatttgaa gaatccacat tcatttcatc
     6301 aaagtataac gcagtaagta tttcaactac tgataatgac cctcctggat gtccagattt
     6361 ggctctatgt atcattgaaa ctatatctct tcttatgata cgcgcaatct catttaatcc
     6421 tttatggtct ctcatgcaat tacctccctt gagttcgtaa ttttatatca attccatatt
     6481 attatatcac ttttatgcta tttttttgcg ttatttttaa ttttattaac atagtacatt
     6541 aaaattaaac cttaagagat atttaaaaga aaaaagggat tgtctaataa aagacaaatc
     6601 cctctttaaa aagtaaacaa tcctaaactt atttgaagtc catcatctac ttttatcatc
     6661 atattttcat ctagacaacc aattttttcc ctcagtctct ttttatcgat agttctaatt
     6721 tgctctaaaa gaattactga gtctttatta agcccatatt cattggaact tatctctaca
     6781 tgagtaggta atttggcttt attaatctga gatgttatag ctgatactat aactgtagga
     6841 ctatatttat tacctacatc attttgtatt atcagaacag gtctaacgcc tccctgctct
     6901 gaaccaacaa ctggacttaa atcagcgtaa tataaatcac cacgctttat ttcacaatta
     6961 gtactcacct tgggcaatct tcccttcatt tttatcatca gaagatactt cttcaactgt
     7021 catacctaat tcagctagtg acagattaat ttgacccatt tctctataac ccgttttcat
     7081 agattcttta atatgaattt tctttttctg agttatatag aactgaaaag cagcttttgc
     7141 aaaatcttct ctgttactgt tctccatctg aactatacta tcaacttcag aaatcagaga
     7201 gtcaggtaaa gtaacttcta ttttttcttt actcttatcg tacacaaaat cgcctcccag
     7261 attataatat catggtttta ttttagcaaa taactgttta tttgtacaag ctcattattt
     7321 tccatataaa cacggtcaac tcttcttgat atcatacata acacttcata gtttatagtt
     7381 cctaagtctt tagctatacg ctcagctgta acttcgcctt ctccaaatag tataacctca
     7441 tctccaactt ttatgtctat atctttgtca attctaacca ttatttgatc catacatatt
     7501 ctaccaacta catcaaacac ttctccctta ataagaacct ttggattttt ttggattcta
     7561 gtaaatccat ctgcgtatcc tattggaact gtagctattg tttctttacc tgtagttgtg
     7621 tattttagtc cataacttat tcctacacct ggttcaactt gttttatatg tccgattttt
     7681 gattttaatt tcatggctgg tcttaattct aatctatctt taaatacatc atcagatgga
     7741 taatgaccat ataatattat tcctgctctt accatattta atcttaaatc agggcaatcc
     7801 ataatagctg cactgtttga tacatgtttt atagctattt ttacaccagc ctcatctaat
     7861 ttatcagaca taaatttata attattagct tgtttataag tgtactcttt acttacttca
     7921 tcagctgtag caaaatgagt aaacatacct tctaaatcta tatattctaa tttatttaat
     7981 tctattattt cctgtactga cttctcatta ggttggaaac ctattctagt catccctgag
     8041 tctattttaa catgaacaca ggctttttct cctaaagatt ttgcaatttc atttatcttt
     8101 tgtgctgttt ctaaagaata aactgtcata gttattttat tttttataga atcttcaaaa
     8161 gcttcgtctg gagtatatcc caagttcaaa ataggaagtg ttatgccatt ttgtctaagt
     8221 tcaattcctt cagcagttct tgctactgct aagtaatcta ctttttcttt ttctagcaat
     8281 tttgcaactt ctactgcacc atgtccatat gcatcagctt ttattactcc acaaatctta
     8341 atatcttctt ctaataaatt tttaatatta tttaagttaa atcttaagtt atctagattt
     8401 atctctgccc atgtaggcac tgttattttt tgcatgtctt tagtccccct tagtttctgt
     8461 attcaaaatc tttgtatttt actgtaaatc ttggcactcc ctctttatct aacacttcca
     8521 ttttttcagg attttttgtg tcagcattta catataatac ttgcttgtta aaatatttat
     8581 tatccccagg aataaaagtc tctaaaacca aatgtccttt ggataacttt actttcatct
     8641 cctcattttg aagatagttt tttataaagt ctcctacaaa aagatattga ttattttttc
     8701 ctgtattagg taactccact acatcactaa tctttggatt tttaactaat attttatcat
     8761 cttgatattc tatacttttc ccttttaaat gttttgggga aattacttct aatttatagt
     8821 tatcggtttt tttataagta tgaattaaaa cataattgtg tgggctttta tttccaacta
     8881 cttctacttc agctttacaa ctgtaataat tcatatcaga aatctgcttt tgaaaatctt
     8941 tgtatacttc ttcttttgtg gactgtcgtt tttggcatcc tattacaatt ataaccagtg
     9001 ccaagaacat aatacatact atggtccact tttttctcac atcacacctc ctacttcagt
     9061 ttattcattg tataaagctt ttattatatc atatctacaa attatacctt taagtttgtt
     9121 gtcatcatct actacaggaa ctctgtttat tgatttttta atcattacat tagctacatc
     9181 atcaaactta tcatcttcat gaacctttat tatatcttta gacataagtt ctccaacctt
     9241 ataagcagct acttgtttta tatctttttc aacttttttt aagtcatcta agaaaatcaa
     9301 tccttgtaat agatttatat agagaggtgc ttctatatat ttttcttttt taagaatatc
     9361 tgtttcagaa attattccaa caactctatt ctctgaatct acaactggca gacctcctat
     9421 tttttctgca attagcatat tagctacatc tgctatacta tcatcttgtt ttgctactat
     9481 tacatctgtt gtcattattt cttttgctgt tttatccatc ataatgtaat cactccttag
     9541 tccttaatta tggttatcgc atttgctata gcataatctt ttgaatgtga tattgaaact
     9601 tttatttcta aaacattata atctatgcac atttttgcta gattattata agtttttact
     9661 attggttttc ccatttcatt tctgagaact tctatatctt taaaattaaa caatctaatt
     9721 cctgtaccta tagatttact tatagcttcc ttggctgcga aattacctgc tatactttct
     9781 gctttaaaat tcttagaatt aaaatactct atttctctat ctgtaaatat cttctccaaa
     9841 aacctattat ttttgtctac agcctttctt attctatcaa tttcaattat atctacgcca
     9901 atatcaaata tattcattgt tcctccagaa aatttataat acttctttca ataattaata
     9961 atgaatttat aaatttacta tcgataatac acattattat ttagtttatg ttacttatct
    10021 taatttaata atcattataa taaaactgta acttttaaaa ttatttgtaa tgaata
//
LOCUS       DAMJZE010000149        10026 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJZE010000149 DAMJZE010000000
VERSION     DAMJZE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.153"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(61..531)
                     /locus_tag="PC541_002982"
     CDS             complement(61..531)
                     /locus_tag="PC541_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2840426.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1401..1790)
                     /locus_tag="PC541_002983"
     CDS             complement(1401..1790)
                     /locus_tag="PC541_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2840427.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3551..4957)
                     /locus_tag="PC541_002984"
     CDS             complement(3551..4957)
                     /locus_tag="PC541_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2840428.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(5002..5709)
                     /locus_tag="PC541_002985"
     CDS             complement(5002..5709)
                     /locus_tag="PC541_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840429.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5880..6794
                     /locus_tag="PC541_002986"
     CDS             5880..6794
                     /locus_tag="PC541_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840430.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6794..7555
                     /locus_tag="PC541_002987"
     CDS             6794..7555
                     /locus_tag="PC541_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840431.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7567..8304
                     /locus_tag="PC541_002988"
     CDS             7567..8304
                     /locus_tag="PC541_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840432.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            9182..9451
                     /locus_tag="PC541_002989"
     CDS             9182..9451
                     /locus_tag="PC541_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2840433.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(9669..9914)
                     /locus_tag="PC541_002990"
     CDS             complement(9669..9914)
                     /locus_tag="PC541_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2840434.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
BASE COUNT         3377 a         1551 c         1088 g         4010 t
ORIGIN      
        1 tattatcttg aattagttct attacaatta atttctacta aataattata atttaaacaa
       61 tcattcctta ctattttcac actgctttac aaatttaatt ggatgttgaa ttattttaga
      121 agtttcttct acagagtatc cctcagataa atatcttcta gctataactt ccatagactc
      181 tcctatctca ataatatgct tatccgggtc ataaatacgc actactcttt gttgccattc
      241 atgttttagt ggcttatgga caaactctac atctttataa taattgagtt tttcaataaa
      301 ttcgtcaaaa tcatcaacct caaaataaag ttccatatta tttgattttt gtataattgt
      361 atctggatta atccctgtaa gccatgcgaa gtcttcttgt atagcaaatc ctcctttaaa
      421 tgttacattt ttgcctaagt ccaattcaat ttcttgctca aataattctt tataaaattt
      481 ttttgatact tcaatatcct tcactgctaa taatgaaacc tgatatttca taaattttcc
      541 tcctaatttt ttatttctat tttttcagta tcttctatta acattttttt ctctatctct
      601 tcagtacctt ttattaatcc tttattttta cttttttagc atcttctatt aacacttttg
      661 tttctaccta ttcagtatct tatatcaatc ttttattttt tcaatatctt ctattaacac
      721 ttttatctct atctattcag tactttctat caatcttttg tttttatgtt ttcaatatct
      781 tctattaaca cttttgtttc tacctcttca gtatcttata tcaatctttt attttttcaa
      841 tatcttctat taacactttt atctctatct attcagtact ttctatcaat cttttgtttt
      901 tattttttca atatcttcta ttaatacttt tgtttctacc tcttcagtat cttatatcaa
      961 tcttttattt tttcaatatc ttctgttaac acttttatct ctatctcttt aatatcttat
     1021 attaatcctt tatttttatt ttttcaatat catcattaat tcctatttat tttatcatct
     1081 taaatacgaa aacactgtgt catatttgca aaatatatat tatcatctat attaaagctt
     1141 cacaatttat atttaaaaat tctctttaat tacactacct ctatttaaaa agctacctta
     1201 attatactta taagtaaagg tagcttttta aatttaatta aatagaatat ccactattaa
     1261 tttatattta atttatttag attcacatta tagtatcata atccacttat aatcatttgt
     1321 catattactt aagtagacta atatattaca taaatacact tacatacact ttatattgat
     1381 gctataagaa atacgtttgc ttatacgtct ttctcctctt cttttagatt ttttatccac
     1441 tcttcaataa gtaaaatatc atctttgctc aaattaatat tttcatgtaa tgacaacaat
     1501 aaacttttta tagaagaatc atgaatattt ctaaaaaaat ctcttgtctc aactcctatg
     1561 tattcctttt ctttaattaa aacttcataa tgcgtatatt tatctatctt ttgagaattt
     1621 agaaatcttt ttactactaa tctagataaa agagtcaaag ttgtcgtttg tttccattga
     1681 tatttttgct ccattcctaa aacaatatct ctagatgtta ctttggaatc tgatttccat
     1741 ataaatttca ttatctttaa ttctgattgt ggcaactttt ttatagtcat gataaaacct
     1801 tgctactcag aatatagaca agcaatatcc ataataaaat ttttcgatta aatacatctt
     1861 atacggattt atataagctc agacttataa gatatttttc aatcttaatc ctaaatttag
     1921 ctatttgtac acggtaaata tatataaata atgtcaaaat tctcttgtat tatattgctt
     1981 aattttatga aatattttgt actcagctat ctattttgga tacttgtcca gtagctctcc
     2041 tttcttttaa actctttgat tttaaatatt tgtatttcag taaatcctac ttcctaaatt
     2101 ttttatttac tacaacttat tactgaactt cctacttcct aaacttttta tttactacaa
     2161 cttattactg aacttctcac ttactaaatt tcttacttac tataaattat aactaaactt
     2221 ctcacttact aaatttctta cttactataa attactacta aacttcctac tttctaaatt
     2281 tcttacttgc tacaaattat tactaaactt cctctcccta aatttcttac ttgctataaa
     2341 ttattactaa acttcctact ttctaaattt cttacttact ataaattatt actaaacttc
     2401 ctactcccta attttcttac ttactataaa ttattactaa atttcctact ttctaaattt
     2461 cttacttact acaaattatt actaaacttc ctactcccta aatttcttac ttgctataaa
     2521 ttattactaa acttcctact ccctaagttt cttacttgct acaaattatt actaaacttc
     2581 ctacttccta aatttcttac ttgctataaa ttattactac taaacttccc acttactaaa
     2641 tttcttactt actactactt ataactaaac ttcccacttc ctaaatttct tacttactac
     2701 cacttataac taaacttccc acttcctaaa tttcttactt actaccactt ataactaaac
     2761 ttcccacttc ctaaatttct tacttactac cacttatcac taaacttcct actttctaaa
     2821 tttcttgctt cttaagaata atgttaattt taattttaag attatagttt ttaccttgtt
     2881 taattgaaac attctatata attatatcct tctattttag agttttttta tttatatatt
     2941 acaaagttta aattttttca ttaattatat tataaatttt ccatatattt caacataaat
     3001 acatatatat atatgttatt tctaaaaata ctacacaaaa actctacttt agtagaactc
     3061 aatggcgcaa aatacttcaa tttaaaacat ttatcgtact ttaaataaat aactataata
     3121 aaatatactt acaatagtta catagaagct attataacta cacagaacag cttataatta
     3181 aaactttttt aggatgtcat tatatttttt cttaaaatta aaaaacacta taccataatt
     3241 ctattttagt agaatttttt tgtcaataat aaaattaaat attaactaaa aaataaaaaa
     3301 aatggaatta tctaaagaat ttaataacct tacaataatt ccaaaatatt atttctatga
     3361 aaattttgat accatgagta actcagccat aagcgcataa agcacaagtt actctttact
     3421 ttaataaaac ttgacaagaa aagaatttat ccttattcta ctctaataga gttaatttgt
     3481 caataaagaa taaaattatt ttcaaaatat tttcattctt cattcttatc tgttattatt
     3541 ttataaaata tcacttaatt agaggtaatt ttagtattgt ttttagtccc tgactatcaa
     3601 tgctcgtaat atctaattta cctctatgtg cattaataat gccagatact atcaaaatac
     3661 ctagaccatg attttgacct ttggcatatg cctcacttag tccattttgg ctctttaata
     3721 aactattaat tctttcttga ctcatacctt ttccatcatc ttcaacaatt atatagcaaa
     3781 aatctaaatc agacttagag gtaaccctta tattacatcc ttgttggtta tgagttatac
     3841 tattttgtat taaattacta atagcccttt ttaataattt tttatctgca tctataataa
     3901 tatcttcatt tagtatatca agttcaacgt aaaagtcatt atccaaatta ttatttataa
     3961 actcagaaat aagctctctc aaaaacgcag aaactctaat cttatcacaa ttcaatgttt
     4021 gcatattata ttcaagttta gacactaaat ttaggtcatt tactaattct ctgattttaa
     4081 tcccttgatt gcatataata gacacttgct cttgttctct atttgaaagg ctactgcttt
     4141 cttcaagttc tccagcatat ccaattatca ttgaaagtgg agttcttata tcatgagata
     4201 ttcctgcaat ccagttagac ctagcttcct ctttcttcct aagtttttca tctttcattt
     4261 gcaactcttt agatacagta ttaatagatt ttgaaatatc acttagtatt cctttttctt
     4321 gtaaaagtac tggttctcct tttgacataa gctttatgcc agtaataatt ggttttatag
     4381 atttcaccat ttttaaacct atgaatagag ctattaaaaa agtaactatt gcattaacca
     4441 gaaacatacc tattattaac ataggaaatt gttctatttc cttaaccgta taagtaaggt
     4501 taaacttagt atacttgtct tttggataac ctgttactaa tattccattt ggatgttccc
     4561 atacatacac aggatagtcc tttaaaaaga accttgaaaa ctttgctaca tctgtcaaag
     4621 tataatgact tggaatttct tttggtaaat caaatcccca tattctatct ccattgttat
     4681 caattaacat tgcccaaact ttatttttat gtagtacttc cttactagaa tcatctaaaa
     4741 taaactttcc atctcttaaa atcaaattat cttcaatcat aatcaagttt gattttattt
     4801 ctctactatc acttgtatct aaattatcag agtatttaga tacaaataac atatatgaaa
     4861 acagaagaaa acctgctata tttaatacaa taaaaagtaa tgaaatcaag atacttgaac
     4921 ctataaatcg cctcaatatg tactttacac tatccaaaat taaatcactc ctcttgattt
     4981 cttttattct aatatctaaa attaattaat catcaactta tatccaagac ctcttactgt
     5041 aataagatgt ttaggtgtag atggagtttt ttctatcttc tctctaaccc ttctaacatg
     5101 aaccataagg gtattttcat atccatacat atcatctccc caaatggctt gacatagtga
     5161 atcattagta acaattctcc ctttattttc atacaacttt aaaagcaacg aatgctcttt
     5221 agctgttaaa gaaatctctt gcccatcttt ctcaacacat gcactattta aattcacaac
     5281 aacagaatct cccagtttaa atactggcaa ctcctcttct tctataggca catatacacg
     5341 atttaatata cccacaagcc ttaaagtaag ttcctttggt aaaaatggct ttactatata
     5401 atcgtcagcc cctaagccca aaccaatcaa tctatcttca tcttcacctc ttgcagagag
     5461 aaaaatcact ggtattttag atgtctctct aatttttctc ataatagaaa aaccatctcc
     5521 atctggcaat acaacatcta aaattgcaca atctggcttt acatttctaa ataccctaac
     5581 agcttcctca taacttgatg ctaaaaatac tctactaaat ccctcttttc tcaggatttc
     5641 atctatcatt tttaataatt ccttgtggtc atcaaccaca agtatttttt tatcttttat
     5701 tgactccata atcatcaccc tcctttagta taccatatac ccattttcac aactttgcct
     5761 aaatagactt ttaaggttat tgtaaggtaa atttcagcta attgaaatgt aaccaagtta
     5821 ttatactgat ataagttaaa agaatcaata agcacagatt aaaaaagggg atgaaacata
     5881 tggaaatgat aattagaaca caaagacttt caaaggaata caataaaata tttagagtaa
     5941 aggatataga cctaagagtt ccacaaggag ctgtatatgg ttttttgggt cctaatggag
     6001 ctggaaaatc taccactctt aaaatgcttc ttggacttgc taaacccaca caaggaaata
     6061 ttaatatttt aggtaaagag ttaaatgaaa aaaatagaat atcaatttta aaagatatag
     6121 gttctttgat agaatctcca tcatactatg gtcatcttac agggcttgaa aacatgacca
     6181 taatgcaaag actcttagat ttacctaaaa aaaatgtaaa tgaagcatta aaaattgtaa
     6241 ggttagaaaa tcaaaaaaat aaaaaagtta gtcaatattc tttaggtatg aagcaacgtc
     6301 ttggcattgc tatggcaata atgaagtttc ccaaattatt aatacttgat gaacctacaa
     6361 atggactcga cccagcaggg attgaggaaa ttagagaatt aataaaatct ctaccagaaa
     6421 aatacgggat gactgttcta atctctagcc atcttttaag tgaaattgac caaattgcca
     6481 catcaatagg aataataaat catggcgaac tggtttttca agatagcata aaagaacttc
     6541 ataatagagg tcaaagtcag attgctatca aaactaagaa tgttgtatct gcacaaaaac
     6601 tattaaatac taaaggatat atgtcactta tacaagatgg gtacttgact attgaaaatt
     6661 taaaagactc agatgtcgct aaaataaatg gtatgcttgt ccattcaaat atagatgtat
     6721 ttagaataga agaacataaa aaaagcttgg aagatatatt cttagaactt actggtaagg
     6781 cggtgacttt ataatgaaat accttggtct agaattttat aaacttaaaa gaaagaagat
     6841 tattctgatg acctttttat ttgcagctgt tgaaataatg tggtgtatcg taaattcaaa
     6901 tagagctatt acacgtaatc cagatatgtt aggtggattt gaatattcct atatgcttat
     6961 gagttttgca agtttaaatg gtctattctt tcctatctta atatcaataa ttacatcacg
     7021 tataagcgat atagaacata aaggagatac atggaagctt ttaaaatctt ctgtgacatc
     7081 tttaaatagt atttatttat ctaaattttt atgttctgct attttggtat ctatacctgt
     7141 attgatgcaa gtgttatcta ttgtgttgtt tggacatttt agaggggtta ttgagtcaat
     7201 ttctgtatct ttacttttaa aatacacatt aggaacaatt cttgtaagta ttgctatcat
     7261 tgcactacac ctatggattg caactgtaat tccaaatcaa atgattgcaa ttacatttgg
     7321 catgattggt agttttatag gtttgacatc aggtttgttt tatcaaggta ttcgtagatt
     7381 gtttatatgg tcttattacc tagagttatc acctttaagt tatgcttatg ataatgtttt
     7441 aggttcatct gtctatagaa ttgatatgaa tttttctatt acgattttag ttttcttaat
     7501 tggaatgcta atttattata ttggaagaaa tcatttatta aaaaaagaag tataggaggg
     7561 atttaaatgt taaggaagtc agttttggga gaaattctaa agttaaaaaa ttcttttata
     7621 tggtatgtat tattagcttt gccacttata agtgtgttga ttggttctgg taatttttat
     7681 ctaaatcaag gcatactcaa aaaagaatgg tacagcttat ggactcaagt tagtcttttt
     7741 tatggggaat ttttctttcc tatcttaatc gcaatctttt gtgcatatat ttgtaggctt
     7801 gaacatatga atcataattg gaataatgta atgacactgc cagttaattt aaaaaatatc
     7861 tttttatcta aacttacagt agtttcaata cttacagcta ttacacaagt tttctttgtt
     7921 atcttttata tagttgcagg aaatatgttt ggtttttctg gtagtgtacc taaagaattg
     7981 actggttgga ttgctaaagg ttggattatt tctatttcaa tagcttctat acaattatac
     8041 atgtctataa agattagaag ttttgcaact cctattggta ttagtctttg cctttctcta
     8101 tttggtatgg gattaatagt agcaaaacct gctgttggta tatttttccc ttattctatg
     8161 ttaggtagtg ctatgggggt tataaagcaa tctgctttaa atgttaatga cactatagtt
     8221 acaatatgtg ttgcatttat tactactata gtattttctg aaattgccaa tagaaatttt
     8281 agaaagaatg atgttatttc ttaatggtaa atgctagata tttttgggtt tgaaatttat
     8341 attcttttta gttgaaatct atatccattt tatttaaaaa tatatatctt ttatttgaat
     8401 tttatatgtt ttatctactc gctatataat agatatactc ctaaaattta aaaatatata
     8461 ataatgttat tatttaaaat tattatgtat tatattaaaa cttaatgcaa aaacaatttt
     8521 tatattatat aatgaaactt aattaaatat aggtaaaaaa tcattcaata tattaaataa
     8581 atatttaact ttaataatct atatttaatt atagctataa catagaatta tcaaatatat
     8641 tgaatgaata tttgactctt ggaatgtgta attaaattaa gtttggttat tcgatggaag
     8701 gcaaatttga tataaaactc catttaaagt gccagataaa aactttatgg ttatataaaa
     8761 gttaaaagta atttatagtg ttatagaaca ttcaaaggga tttaaagtaa tctatggtgt
     8821 tttgaaatat tcaaagggat ttaaactaat ctatagtctt gtagaatatt caaagagatt
     8881 taaaacaatc tataatgttg taaaatatat tcaaagggat ttaaactaat ctatagtctt
     8941 gtagaatatt caaagagatt taaagcaatc tataatgctg taaaatatat tcaaggggat
     9001 ttgaagtaat ttattgtgtt ttgaaatatt caaaaagatt tttggaatag tttttataaa
     9061 tttaccagta ctaataattt tatgtttaac atttagatga cctaaaatca actatttgca
     9121 cttttgtatt catctaaaat cattttataa tatttttaaa taatggaaag gatgtttgaa
     9181 tatgtttgaa tcaagatgtg gtgtctgttg tgacagttgt acaaaaaaag aacaagtaaa
     9241 ttgcactggt tgcccaacta tggaaaaacc attttggggt ggagaatgta aggttaaaac
     9301 ttgctgtgaa agtaaggaac ttaatcattg tggtgaatgt gatactttcc cttgtgatat
     9361 gcttttaaat aagggaaaag accaaggttt tgaccctatg gtaaatattg agcaatgtag
     9421 aaaatggctt gaagaaacag tttctaatta attgttttag aatgctttgt taaggaattt
     9481 atttaatcct ttgttaggta gtatgtttag tcatttgttg attagtatat ttatacattt
     9541 attgatgggt atatttagcc atacaaatag atttacataa aatttacgac taaaaaagag
     9601 ttgagtatta gtttttatta tttaaaacgg atattcaact cttttttatg tctaactttt
     9661 ttgatttttt aatctttcca ataccaccac tttaattttt ctggttctgg attttcagtt
     9721 gatgcaaaat atactgcaca tctgtataca tataactgac atctgtctat gtgctgtcca
     9781 cgcattaaac attccttttc atacatttgt gctgggtttg catctttaag tgatttaata
     9841 gttgtatatc ctaacatgat taaagatttt tctgttgctt tccctacccc tggaattttt
     9901 tgtagttctc ccatacttca ccttctttct ggtatattct ttttatatta tacctattac
     9961 cagatatttt tttattatag ttaatattta atatttaatt ttttattata tcaatttatt
    10021 tatgct
//
LOCUS       DAMJZE010000150         9890 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJZE010000150 DAMJZE010000000
VERSION     DAMJZE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.154"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..118)
                     /locus_tag="PC541_002991"
     CDS             complement(<1..118)
                     /locus_tag="PC541_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2840435.1"
                     /translation="MAYDFYLDGVQLPIAPGKLEIKVTNKNKTVDLINTGEVN"
     gene            complement(130..2553)
                     /locus_tag="PC541_002992"
     CDS             complement(130..2553)
                     /locus_tag="PC541_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2840436.1"
                     /translation="MTTSINTTINSLERLQRELRNPLDEGGIQASVKIFDRMAPALKN
                     MNTSINATAKSLERLQQKLNNPINAGNIQASQQNLKNIESILTRIEQKIGRNTNEQEN
                     FNNKIRKGSEEGSLLVSKLKSLAGVYIGIRGIESITKAADTIASTKARLNLMNDGLQT
                     TEQLNKMIYLSAQSARASYADTAAQVAKLGILAGDAFGSSAEVVKFTELMNKAFVIGG
                     TSASEASAAMYQLTQAMGAGKLQGDEFRSIMENAPLLATKIADAMGKTKDQLKELSSS
                     GAITADVIRNALFKASDEIEKKFASMPITFSQALTMMKNDAYMIFGQTLGKISGALQS
                     VRFSEIVVSMRNVMIAISSNIYDTLNIIKNILNSDFFSDFVQGITLGAVLIIQGLGGI
                     TNAALSVVNVFAQNWNIIEPIVYGVVAAIAIYSLALAGMWLWEKLVAASKFAMMFAQM
                     LLNTKTLAGYAYLIKYAAGQWIANEAMLACPIFWIVAGILAFIVVVFVAVAAVNKFAG
                     TNLTALGVIVGAVFAAVAFIQNIMIWLFNRCVDVNEGITNGWNQCVFFMKQAIAKGVI
                     FIIEKMASLNDSVNNAGNALGKAFVTGANIAIRGVNKLIDLLNKIPGINIGKVGEATF
                     TPVKADNSYIKQQINSLNRWVGDAPEKIKLERMQYKDIGAEFQKGNALGTKWQNAISN
                     KLKDTFDINKMLEDAKDKLGLKDLWDKNNPLNNLGGFGGDLGKNVKDTAGNTAKMAKT
                     MDKSQEDLKYLRDIAEQEVINRFTGVNIKIDMNNTNNISKDADVDGIVNILTEKLNDA
                     MVVSAEGVV"
     gene            complement(2682..3443)
                     /locus_tag="PC541_002993"
     CDS             complement(2682..3443)
                     /locus_tag="PC541_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2840437.1"
                     /translation="MGLFRKSKEPCCICGKEKTDQKIKDGSVCIECLMNYMEYKDKQF
                     TFKDGLPTKSEIKSVLDSKKQNDFLVEKFNVTKEINSFIKFDETNKLIYIETKRKNGK
                     IKKNVYSFEDITGFELLEDGETVTKGGIGSALAGGVLFGGTGAIVGSVVGKKKTKKII
                     ENLQIKLTLKAISEPVAYINLISGKTKTNSIGYERAYEDAQEILSVLSIILKDTENNS
                     NNSNADEILKYKNLLDLGAITEEEFNIKKKELLNL"
     repeat_region   3569..3796
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3634..3664
                     /rpt_unit_seq="atttacataccacatagttaatataaaataa"
     gene            complement(4074..4232)
                     /locus_tag="PC541_002994"
     CDS             complement(4074..4232)
                     /locus_tag="PC541_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840438.1"
                     /translation="MDNFLQGVLASLVASLIVYLTSKLFKKVKSHSNTTKSDFDFKLT
                     IKFKRNKH"
     gene            complement(4555..4995)
                     /locus_tag="PC541_002995"
     CDS             complement(4555..4995)
                     /locus_tag="PC541_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2840439.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKPIEWELKAIDSD
                     RDRQLRKNSAVRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            complement(4997..5254)
                     /locus_tag="PC541_002996"
     CDS             complement(4997..5254)
                     /locus_tag="PC541_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840440.1"
                     /translation="MVKIKDKYEIKDSISFDYSKKRPIEECVGEMYRKAGLFLVDIAD
                     KLALDTIEGSSLKPITIKIKLDEDGIATIEKETKYLIMEVE"
     gene            complement(5307..5783)
                     /locus_tag="PC541_002997"
     CDS             complement(5307..5783)
                     /locus_tag="PC541_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2840441.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            complement(5802..7112)
                     /locus_tag="PC541_002998"
     CDS             complement(5802..7112)
                     /locus_tag="PC541_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2840442.1"
                     /translation="MALGGGTFVAQNKVLPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHDKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATAKYSGTRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLVYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSIRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMGKLYMTVSVS"
     gene            complement(7113..7289)
                     /locus_tag="PC541_002999"
     CDS             complement(7113..7289)
                     /locus_tag="PC541_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840443.1"
                     /translation="MSKTLSKETDYKFTKEQIVNSKKYVNRKDLLNAILRENELYSFS
                     EVEKIINNFMKGVS"
     gene            complement(7282..7719)
                     /locus_tag="PC541_003000"
     CDS             complement(7282..7719)
                     /locus_tag="PC541_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840444.1"
                     /translation="MINNIIDGISVKLDKSFGNEYTIYSEDVEQGINEPCFFIVPINS
                     SKALYPNGRELKKNSFDVHYLPRSKDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(7712..8143)
                     /locus_tag="PC541_003001"
     CDS             complement(7712..8143)
                     /locus_tag="PC541_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2840445.1"
                     /translation="MARGGSVDFKELKKLQRKLQRLEDSQLDKFLKDCARELAARLVR
                     KARKRGRTPKKTGTLKDGWGGIAYARKLPVTKVGDNYVIEVINPVPYASFVEYGHRTR
                     NHKGFVKGKYMMTISIMELRGEAEAIIEKKLMVLLKKVFDD"
     gene            complement(8143..8505)
                     /locus_tag="PC541_003002"
     CDS             complement(8143..8505)
                     /locus_tag="PC541_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840446.1"
                     /translation="MVNRTRKAIEMMYRDKCTIVEYQPIKDPITKRTNNKEVVVLENQ
                     PCKLSYKNTTSATDGKVAKLTQTIKLFISPNIEIKAGSKLIITNQNNITKEYIRSGEA
                     AIYPNHQEIILELLEDKA"
     gene            complement(8499..8900)
                     /locus_tag="PC541_003003"
     CDS             complement(8499..8900)
                     /locus_tag="PC541_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840447.1"
                     /translation="MENNLIDDIEKRLESFEYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESLNFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDTLVNYLLTYGKNKILTFRCLRW"
     gene            complement(8910..9143)
                     /locus_tag="PC541_003004"
     CDS             complement(8910..9143)
                     /locus_tag="PC541_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840448.1"
                     /translation="MPQVKKLNRILTIEECRIDDFLEMGYDLIDETGKVVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(9162..>9890)
                     /locus_tag="PC541_003005"
     CDS             complement(9162..>9890)
                     /locus_tag="PC541_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2840449.1"
                     /translation="VDSNRDTIAVAQRNYDNAWESKTLTNQRKWSTLVHPADIDQTNY
                     VASIGNITKVYNEEQKFPEMDAYCISKIYADWIALGKTAETTALTTANILEVFDSLME
                     KMTEARVPAVGRILYVTPGVDTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDSVKIVKV
                     PSNLMKTVYDFTTGWKAGAGAKQIFMSLIHPSAIITPVSYQFSTLDEPRAVTEGKYLY
                     FEESFEDVFILNKKADAVQFVVEA"
BASE COUNT         2948 a         1959 c         1070 g         3913 t
ORIGIN      
        1 tgtttacttc tccagtattt atcaaatcaa ctgtcttatt cttgtttgtt actttaattt
       61 caagtttacc aggtgcgatt ggtaattgta ctccatctaa ataaaagtca taagccattt
      121 atatccctcc taaactactc cctcagcaga tacaaccata gcgtcattta atttttcagt
      181 taaaatattg actataccat ccacatctgc atctttactt atgttgtttg tattgttcat
      241 gtcaatttta atgtttactc ctgtgaatcg gtttattacc tcctgctctg caatatctct
      301 aagatatttt aaatcttctt gacttttatc cattgtttta gccatttttg cagtgtttcc
      361 tgccgtgtcc tttacatttt ttcctaaatc tccaccaaat cctccaagat tattaagtgg
      421 attgttttta tcccacaaat cttttaatcc tagcttgtct tttgcatctt ctagcatctt
      481 gttaatgtca aaagtatctt ttaatttatt agatatagca ttttgccatt tagttccaag
      541 tgcatttcct ttttgaaatt ctgctccaat atctttatat tgcatccttt ccaattttat
      601 tttttctggt gcatctccta cccatctgtt taaactgtta atctgttgct taatgtaact
      661 gttatccgcc ttaacaggtg taaatgttgc ctctcctact ttcccaatgt taatcccagg
      721 gattttattt agcaaatcta ttaatttatt tacacctcgt attgctatat tagctccagt
      781 aacaaaagcc ttaccaagtg cattacctgc attattcaca gagtcattca atgatgccat
      841 tttctcaatt ataaagatta caccttttgc aattgcttgt ttcataaaaa aaacacactg
      901 attccaacca tttgtaatgc cttcatttac atctacacat ctattaaaca gccatatcat
      961 tatgttttgt ataaatgcta ctgctgcaaa tactgcaccc acaattactc ctaaggcggt
     1021 taaattcgtt cctgcgaatt tgtttactgc tgctactgca acaaaaacta ctactataaa
     1081 tgctagaatg cctgcaacta tccaaaaaat cgggcatgct aacatagctt catttgctat
     1141 ccattgtcca gcagcatatt ttataagata cgcatatcca gctaaagttt ttgtatttaa
     1201 aagcatctga gcaaacatca tagcaaattt agatgctgca actaattttt cccataacca
     1261 cattcctgcc aaagctaaag aataaattgc aatagctgcg acaactccat aaacaatcgg
     1321 ctctattatg ttccagttct gtgcaaaaac attaacaacg ctaagtgcag cattagtaat
     1381 tcctccaagc ccttgtatta ttaatactgc tcctaatgta attccttgaa caaaatctga
     1441 gaaaaaatca ctatttaata tattttttat aatatttaat gtatcataaa tatttgaaga
     1501 tattgcaatc attacattcc gcatagacac aacaatctca ctaaatctaa cgctttgcaa
     1561 agctccactt atttttccta atgtctgacc aaatatcata taagcatcat ttttcatcat
     1621 tgtgagagct tgagaaaaag tgattggcat acttgcaaat ttcttttcta tctcatcaga
     1681 agctttaaac agtgcatttc ttataacatc tgctgttatt gctccactac ttgataattc
     1741 cttcaattgg tctttagttt tccccattgc atcagctatt ttagtagcta gaagtggagc
     1801 attttccatg atagaacgaa actcatcacc ttgaagtttc ccagcaccca tagcttgagt
     1861 aagctgatac attgctgcac tagcttcact tgctgatgtc cctccaatta caaaagcttt
     1921 attcataagt tctgtaaact ttaccacctc tgctgaactt ccaaaagcat ctcctgctaa
     1981 tatcccaagt ttagcaacct gtgctgctgt atctgcataa ctagctcttg cactttgagc
     2041 agacaaataa atcatcttat tcagttgctc tgttgtctgt aagccatcat tcattagatt
     2101 taaacgtgct tttgtactgg caattgtatc tgctgctttt gtaatacttt ctatacctct
     2161 tattccaata tatacacccg ctaagctttt aagtttagat actaatagag aaccctcttc
     2221 acttcctttt cttattttat tattaaagtt ttcttgttca tttgtatttc ttccaatttt
     2281 ctgttctatc ctcgttaaaa tactttcaat attttttaag ttttgttgtg aagcttgtat
     2341 attacctgca tttataggat tgttcaattt ttgttgtaat ctctctaaac ttttagctgt
     2401 tgcattaata gatgtattca tatttttaag tgcaggtgcc attctatcaa aaatttttac
     2461 agatgcttgt attccacctt catctagagg atttctcaat tctcgttgta atcgctctaa
     2521 actattaatc gttgtgttaa tggatgtagt catgtttcta aatgcaggtg tcattccgtc
     2581 gaaaatcttt attgaagttt gtattgtagc catttcctca ctctcctttc ttttttgaaa
     2641 taataaaagc acttacttat ttttaagtaa gcgcttatgt attataaatt caatagttct
     2701 tttttcttaa tattaaattc ttcttctgtt attgctccta aatctaataa atttttatat
     2761 tttaatattt catctgcatt agaattattg ctattatttt ctgtatcttt taaaattata
     2821 gataaaacag ataaaatttc ttgtgcatct tcatacgctc tttcatatcc aatagaattt
     2881 gtctttgttt ttccactaat taaatttata tatgccacag gctctgaaat agcttttaat
     2941 gtaagcttta tttgtaaatt ttcaattatc ttttttgttt ttttcttgcc tacaacgcta
     3001 ccaacaatag ctcctgtacc accaaacaag actcctcctg ctaaagcact tccaatacca
     3061 ccttttgtta cagtctctcc atcttctagc aactcaaaac ctgttatatc ttcaaaacta
     3121 taaacattct tcttaatttt tccatttttt cttttagttt ctatatatat gagtttatta
     3181 gtttcgtcaa actttataaa actgttaatt tctttagtta cattaaattt ttcaactaaa
     3241 aaatcatttt gtttcttaga atctagaaca cttttaattt cagattttgt aggtaatcca
     3301 tctttaaatg taaattgttt gtctttgtat tccatataat tcattagaca ttctatgcaa
     3361 actgaaccat cctttatttt ttggtctgtc ttttcttttc cacatataca gcacggttcc
     3421 tttgattttc taaataatcc cataatatta cccccatata ataattttat aagattatta
     3481 tactatataa gtaaaatttt tacatcataa tcacctcctt tcaataaaaa aacacctact
     3541 attcaagtaa gtgtttttag cgtttttaat tttaagtcca catagttaat ataaaacatt
     3601 tttaattgaa gtccaaattt gagaaaatgt gtaatttaca taccacatag ttaatataaa
     3661 ataattatgg gtagatttta aagataaagt aaaaaatgga tttacatacc acatagttaa
     3721 tataaaataa ggtaatactg taatagaacc agaatttgat tatctattta cataccacat
     3781 agttaatata aaatcccaaa ataaacttag catttccaat acctacacat acgcatctct
     3841 ctcaaatttg cagtgaacca tgagtagtgc aattgataac atttatcaca caccctcaac
     3901 gccttatatt tcaatgctta agccttaatt tcttcaaaaa atcgaacact gcaaaatttc
     3961 tatgccttta ttatatcata aaaatattat ttttgaatat ctgtaccaat ttgtggtata
     4021 ataaaagcaa gaagaactac aatctatttg aaactagagt gaagttctaa aaattaatgt
     4081 ttatttcttt taaacttgat tgtaagttta aaatcaaagt cactcttagt cgtgtttgag
     4141 tggcttttta cttttttaaa taacttacta gttaagtaaa ctattaaact ggcaactaaa
     4201 cttgctagta caccttgtaa aaaattatcc atacatactc acctcccttc tatacgttgg
     4261 gaggataatc ttttgtatga actccactct ataaattgta gattacatct tcttgctaaa
     4321 aatattataa catataattc ttacatattt tacctatatt ttatcttctt ctacttcttc
     4381 tagcttcttt agcagtcttt ttttcttctt ttatttcttc ttctacttta atatctatag
     4441 aagcagcaac aaatgccctc tcgtagtctg gtaaatctgt atattcatgt ggtttccatt
     4501 tgaatttatg aaggcaataa tgagctgcac tagcatcata atcgcctcct tcaattagtt
     4561 ttttgcttct tctactttat cctcaaaagt cctatcaaaa ccatttacct ctcctacttc
     4621 acttgaaagg tctgtgtatt cgccaggagt taacattgtt gttaatagtt cctctgctcc
     4681 cattacacca tagctatttt gaagttctgc atcatgtaaa tctggaaata ctatagtttc
     4741 cacacataat ttcaaagtgt aagtattaaa atctgtttca ctagtgtatt gccctgttgc
     4801 ttttcctttt ttgcctataa caggaactct aacagcagaa ttttttctca actgtctatc
     4861 tctgtctgaa tctattgctt taagttccca ttctattggt ttaccatcct cacctataaa
     4921 cctttcactt gccacatact ttctattctc tacttttatt gcattttgac ttaaaaaagc
     4981 gtttaaatct cccatattat tctacctcca tgattaaata ttttgtttct ttttctattg
     5041 ttgcaatgcc atcctcatct agtttgattt ttattgtaat tggttttaat gatgaacctt
     5101 caattgtatc aagtgccagt ttatcagcaa tatcaactaa aaatagacct gcttttctat
     5161 acatttctcc tacacattct tctataggtc ttttcttact gtaatcaaaa gaaattgaat
     5221 cttttatctc atatttatcc ttaattttaa ccatctccta ttcttataaa taaaaaatac
     5281 acatttatga tttatagatg tgtattttac tccataccat ttgctatatt gaacttctcg
     5341 actattctcc aattctcaaa agtaaaatct acatcctcat ccaaatattc tccatcagcg
     5401 tcaaatttag caattatacc tgaatctagg ttacaatctt caagtattat agtttgacga
     5461 cctactgaac ttgttgggtc ttcgttagta atttgcatat caaagtaaat atcctcgcct
     5521 gtttctttat attcatataa caactctcta aaaatagaag tattataata aaaggtagca
     5581 ctccctgaaa atttacttcc tgtactttta tttccttttg taatgctacc taaaattggc
     5641 atctcacttt tatttttttc catcttagct tctaagttaa tagcttgcat aaaattatat
     5701 cttttgcctt ttattgttac aaaacattca gctttagaac cacttattgc atctctaaca
     5761 ttcatagtaa tatttttaga catctatatc actctccttt cttaactaac tgaaacagtc
     5821 atataaagct tacccatagc actaataact tttacagcat cacttactac aacagttttc
     5881 ttgtcgcttc caggttctac agaaacatca tcagctttga aatcttctat tgctctcata
     5941 ttttgcagtt gttcatgatg cttaactaca tcattccaaa acgagatacg accagattta
     6001 tcatttggta cttcgcccaa gtactttgta ttaaataaag tcgctatatc attagcaatc
     6061 tggtcaagta ctctaatact ttgattagat gaaaaatcgt cattcttatc gtctgtaaaa
     6121 cttacaaaag tatttatgtc ctctaataca tgaacttcat ctcccacctt gtggaatata
     6181 aatttaccag tttttaaagc ttcttcaagt tgtatttgag tataattaac atcaacatca
     6241 aactcaccat catacttctt atttgtatta gatttattta tgtcacatcc tgctatagct
     6301 ccagtagtcc aataaactaa actagattct aataacccag tatctttaat cttattttct
     6361 acagatacta caccttcata atctgcatca tttttcttat atagtactgt ttgaaactta
     6421 gctcctacct tatctctcat tctctttgta aattctacaa acaaactttt aatttctgct
     6481 gttgtagcca aacaccctag tgcattaaat gaataacttt ctattttatc caagaaagct
     6541 tggtactctg ctcctgtcac agcttcgcca ttagttccac cagtaaatac aagtcctgcg
     6601 cttgcttcta atgttgcatc cttcttccaa gtgatatagt cattatcttg taagtctgta
     6661 atcactttag ctatttgagt atctaccttc ttattatcta aaagtgttac aacatcaaac
     6721 ttagtgttat catctatatt tgttgttact ataactttta aatcattacc tctagtacct
     6781 gagtacttag ctgtagcaat actgcaacta gctttaacac ctttattcaa tttataaaaa
     6841 tatcccaacc ttatattttt aaataaatct ctaagacctt tcaacttatc atgtgtataa
     6901 tcatatccaa aatacttagt tgaatacttc tcaaaatcat cactggttac ttgaaatact
     6961 tcttcatcta tgccccagtc tagctccaaa ggcattgcca caatacctct atccgataat
     7021 gaactggttg ccctcttagc tgagataaaa tttatatatg caccaggtag gaccttattt
     7081 tgcgctacaa atgttcctcc acctaaagcc atctaactca ctcctttcat aaaattattt
     7141 attattttct ctacttctga gaaggaatat aactcatttt ctcttaaaat tgcatttaat
     7201 aaatcttttc tatttacata cttcttagag ttaactatct gctccttagt aaatttgtaa
     7261 tcagtttctt tgcttaatgt cttactcaaa attatcacct ctcttcaaac caccgaataa
     7321 ctctactgta tccatcttat ctgtatcatt actttttata gtaaagtagt tataatcaac
     7381 gaagaaatga agaacattat ctacaatttc aaagttcata tttgtacctc taactaaatc
     7441 tccatcaatt tctatatact ctaattcctc caataacatt tcagctatct catttatttc
     7501 aaaattctta tcttttgaac gtggtaagta atgtacatca aatgagtttt tcttcaactc
     7561 tctgccattt ggatataatg ccttacttga atttatagga acaataaaaa aacaaggttc
     7621 atttatacct tgttccacat cttcgctgta aattgtgtat tcatttccaa acgacttatc
     7681 taactttact gatattccat caattatatt attaatcatc aaataccttc tttaataaaa
     7741 ccattaattt tttctctatt attgcttctg cttcccctct taattccatt attgaaatag
     7801 tcatcatata ttttccttta acaaaaccct tatgatttct agttctatga ccatattcaa
     7861 caaaactggc ataaggaact ggattaatca cctctataac ataattatct ccaactttag
     7921 taacaggaag cttcctagca taagctatcc caccccaacc atcttttaga gttccagtct
     7981 tttttggtgt acgtccacgc ttcctagctt tacgaacaag tcttgcagct aattctcttg
     8041 cacaatcttt taaaaattta tcaagctgac tatcttctag cctttgcaac tttctttgta
     8101 gcttcttcaa ttctttaaaa tcaacacttc cacctctagc cattacgctt tatcctctag
     8161 taattctaag atgatttctt gatggtttgg atatatggca gcttctccac ttcttatata
     8221 ttcttttgta atattatttt gattagttat aataagtttt gaacctgctt taatttctat
     8281 atttggagat ataaagagtt taatcgtctg tgtaagttta gccacttttc catctgtagc
     8341 tgatgtagta tttttatacg aaagcttaca aggttgattt tctaatacaa ctacttcttt
     8401 gttatttgtt cgctttgtta tagggtcttt aattggctga tactcaacta tagtacattt
     8461 atctctatac atcatttcta ttgcttttct agttctgttt accatcttaa acacctaaag
     8521 gttaatattt tattcttacc ataagttaaa agataattta caagagtgtc aaagcgttgt
     8581 tctggtgttt gagagccact tcctatagca aaatctacct ttgtatcacc ttctgatata
     8641 gacttttcta cagcttcaaa attaagactt tctatatcta attgccccat atttttcttg
     8701 gtaaataaga actctccaac tatcatatca acttcaattt ctttcaattc aattggcata
     8761 gtttttatat tacaatctag tttaataata ttttctattt tttctcttac aaaacctatt
     8821 aaccacttat ccccatcttt taatatatat tcaaaacttt caagtctttt ttctatatca
     8881 tcaatcagat tattttccat aattttcacc tactttttag taagtttatt tttctcttta
     8941 agctgcttat tttcttcttc taaagactca acttttgacc ttaaaatatt attttcagct
     9001 attaaatctt ttacatttaa tgacttgcca tactttacta ccttaccagt ttcatctatc
     9061 aaatcatatc ccatttctaa aaaatcatct attctacatt cttctatagt taatattcta
     9121 tttaatttct ttacttgtgg cattatatat cactcccttt tctatgcttc aacaacaaat
     9181 tgtacagcat cagctttttt atttaatata aatacatcct caaaactttc ttcaaagtat
     9241 aaatattttc cttctgtaac tgcccttggc tcatctaatg tagaaaattg ataagaaact
     9301 ggtgtaatta ttgcacttgg atgtattaaa gacataaata tttgcttagc tcctgcacct
     9361 gctttccatc cagttgtaaa atcatatact gttttcataa gattagatgg tactttaact
     9421 attttaactg aatcaatatc tgtagtttgt ctatttaaag atgttcctcc atcttttatg
     9481 tttacagttc tttgtatttc ttttgcattc ttaatcaaag tatcaacgcc tggtgtaaca
     9541 tataatattc ttccaacagc aggaactcta gcttctgtca ttttttccat taggctatca
     9601 aaaacctcta agatattcgc tgttgtaaga gcagttgtct ctgctgtttt acctagagct
     9661 atccaatcag catatatttt agatatacag taagcatcca tctcaggaaa cttttgttcc
     9721 tcattatata cttttgttat attgcctatt gatgctacat agttagtttg gtcaatatct
     9781 gctggatgaa ccaatgtaga ccattttctt tggttagtta atgttttaga ttcccaagca
     9841 ttatcatagt ttctttgagc tactgctatt gtatctctgt ttgaatctac
//
LOCUS       DAMJZE010000151         9602 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJZE010000151 DAMJZE010000000
VERSION     DAMJZE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.155"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            144..1508
                     /locus_tag="PC541_003006"
     CDS             144..1508
                     /locus_tag="PC541_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2840450.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1530..2021
                     /locus_tag="PC541_003007"
     CDS             1530..2021
                     /locus_tag="PC541_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2840451.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2294..2965
                     /locus_tag="PC541_003008"
     CDS             2294..2965
                     /locus_tag="PC541_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2840452.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3050..3916
                     /locus_tag="PC541_003009"
     CDS             3050..3916
                     /locus_tag="PC541_003009"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2840453.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3949..5451
                     /locus_tag="PC541_003010"
     CDS             3949..5451
                     /locus_tag="PC541_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2840454.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5581..6033
                     /locus_tag="PC541_003011"
     CDS             5581..6033
                     /locus_tag="PC541_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2840455.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6193..6996
                     /locus_tag="PC541_003012"
     CDS             6193..6996
                     /locus_tag="PC541_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840456.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7054..7941
                     /locus_tag="PC541_003013"
     CDS             7054..7941
                     /locus_tag="PC541_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2840457.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7960..8808
                     /locus_tag="PC541_003014"
     CDS             7960..8808
                     /locus_tag="PC541_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2840458.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8871..9560
                     /locus_tag="PC541_003015"
     CDS             8871..9560
                     /locus_tag="PC541_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2840459.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         3873 a          909 c         1685 g         3135 t
ORIGIN      
        1 aactatttaa atatcaagac ctgttaaaaa acaacagcaa taaacaaata aaatagataa
       61 atatatccaa ttaaggtata attaagtttt atatgatata gttatatttg ataatttaca
      121 atagtaaaac ttggagggaa aaaatgggtg aaaagaaaat aaataatatg aattattcac
      181 ataataatta ttccaaaaag acaggtgaac caactagaaa aataaaaaat agccatatca
      241 ctcttaaaag taaagaagaa aaaatttgtt ctagttgcaa taaagaaaat aatactaagg
      301 ataattactg taaattttgt ggaaatgaac tttatgaaat agcatcatta agaccacttg
      361 aaacaaaact agacttgaaa tctaaaataa aagagctttc atatcatgca aataaaagag
      421 gtgtattttt aacaacattt acaaccatat ttatattgtt tattattgca ttgatattta
      481 aagctattat aactattcaa tttaatgata ttagttattt aataaatcca gcacatataa
      541 tcctagctct caacttgggt caaattagtg tatctatgtc taccatgatg ggttctgggt
      601 ttataaatgc caatataggt ttgttgatat tacttataat ccctattttg gtattattaa
      661 tatcaaacct aatttttatg agaaaaagat gtagagattc aaaaactgta ttagcaaatt
      721 ctctaggagt tggaattttt tatggattag ttttagctat tttatctatt tttactaatg
      781 taaaaactag ttcacatagt atgttagaat atggatatgc attagaatat agttatgaat
      841 tttttagtgt tcttttaaat ggatttgtat tagggtttat atgtacatac ataactactt
      901 ataaaaaaag ttatgaaaag gaaaatatgt atttatctct tttttcaaat tctataagaa
      961 cattcatatt aggttatgtt ttagtattag taatactatt ggtactaact atatctgata
     1021 gtagttactt aaatgaacta gatatgagta gttattcaaa tggattaaat ttatttacta
     1081 tactcccaca aatagctagt tatatgtggg cttttgcaaa tggaatttca gtaaccataa
     1141 taaactcaac agtatcaatg tttactttaa gttcttcatc tttatttgga gatactaaat
     1201 taatgtttta tgcaatggga gcattgtcta tgttaatatt attattaaat ggatacaagc
     1261 taagatttaa gtataataca gatagtataa gacctataat agtatttagt atatattatg
     1321 catttttgat gggaatattg gctttattta gtacatttat actagatagt aatattaatt
     1381 tctttaatac aactaattat ggaacaactt taataatgca atttaaagtc ttacaagcta
     1441 ttgtaatttc atttgtatat agttttgtga tatctttaat aggatataag ctaaattcag
     1501 ctgattaaat atgattggag ggaatttctg tggaaggaaa tactaataat aaagtagatg
     1561 ataaattttc taaggttaag aaaaagtttg gtagtggaaa atttaaagtt gaattatata
     1621 aagaaaaatc aaaattaaat agagagattg atgatattca agaaaagaaa gcaaagatat
     1681 tcttggaaat gggaatactg acatatcaaa aaattagaga agattctatt aatgataata
     1741 gttttgataa attctgtgat gagttactag aacttgataa gttaatttat gaaaaaaata
     1801 tggaaattaa tgaaatggaa atggcagaaa gtaatgtaac ttgtgagtgt ggttatgttg
     1861 gaaatgtaaa tgataaatat tgtgctgaat gtggtaaaaa atttgaattg gaaaatgaat
     1921 gtgatgattt tataatttgt ggttattgtg aaagtgagat agatacagag gctgaatttt
     1981 gtccatgctg tggaagaaaa attataatgg atttagaata atattgggaa acctatgtat
     2041 agtatacatg ggttttttta ttgaaattat ttaaatggac ttataacagt agttatataa
     2101 aatgaaaata aacaccaaaa aaatgaaata aaatttcttt taaatgtaaa aaacaagcta
     2161 atatataaaa taaatcagta aaaatataga tgcgagaaaa aaatatgaaa aaatattgca
     2221 aagaaaaagt tttcatgtta ttattaatac ataaatcaag aaaataaatt tcagaaacga
     2281 ggtaatttaa aaaatgctag ataaggtaaa gggaagatta atagtatcat gccaagcttt
     2341 agaagatgaa cctcttcata gtccttttat aatggggaga atggctaagg ctgctatgga
     2401 aggtggagct gttggaatta gagctcaagg agttgaagat attattgaaa taaaaaaagt
     2461 tactggactt cctgttatag gtattataaa gagaaactat gaagattcag atatatatat
     2521 aacgccaact aaaaaagaag ttgatgaact tttaacaaca gggtgtgaaa tgatagcatt
     2581 agatgctacc aatagagtta gaccaaataa tgaagactta aaagaactta taaagtatat
     2641 aaaagaaaac ggtgttctag taatggcaga tatttcaaat tatgatgagg ctatcaaagc
     2701 acaagagtac ggtgtagatt gcgtttcaac tacactatct ggatatactc catatactaa
     2761 aacattagaa ggtccagatt ttgtattaat ggaaagatta gttaaagatt tagaaatacc
     2821 tgtaatagct gaaggaaagg taaatactcc tcaagattta aaaaaggtct ttgagttggg
     2881 tgttcattca tcagtagtag gttcagcaat aacaagacca caattaataa cagaaaaatt
     2941 tgttaaagca attgaaatta attaaaaatt aatttataaa ttaaaaaatt attatattta
     3001 actatatttg aaaattaaaa ttggaggtaa agatatgaga acaacagata tgaagggaat
     3061 ttattcagca ttattagttt catttgataa ggaaggaaat ataaatgaaa aagggttaag
     3121 acaaataatt agacacaata tagatgtttg taaagtagat ggtctttatg taggaggaag
     3181 tacaggggaa aacttcatgc tttcaactga tgaaaagaaa agaatatttg aaatagctaa
     3241 agatgaagtg aaagaagaaa taaaattaat agctcaagtt ggttcagtta atttaaaaga
     3301 agctgtagaa cttgctaaat ttacaactga tttaggatat gatgcaataa gtgctgttac
     3361 acctttttac tataaatttg attttgaaga aataaaacac tattataata caataataaa
     3421 ttctgtagat aatagattaa taatatactc tataccattt ttaactggtg ttgatatgag
     3481 tttagaccag tttggagaat tatttgaaaa tgaaaaaata ataggtgtta agtttacagc
     3541 agcagatttc tacttattag agagaatgag aaaaacattc ccaaataaat taatatttgc
     3601 aggatttgat gaaatgatgt taccagctac agttttaggt gtagatgggg caattggttc
     3661 tacattcaat gtaaacggtg tcagagctag acaaatattc gaattaacta agaatgaaaa
     3721 aatatcagaa gcacttgaag tacaacatgt gactaatgat ttaataacag atatattagg
     3781 aaatggctta tatcaaacaa ttaaattact acttgaagaa caaggtgttg aggctggata
     3841 ctgtagacaa cctatgaaag aagcaactga tgaaatgaaa tcaagagcaa aagaaatata
     3901 cagaaagtat ttttaataaa atacttttag tatgggagga ttagtcaaat ggtaggattt
     3961 acttggatag attttgcaat actagtggtt tacttattgg cagttttatt tgcaggattg
     4021 ttattttcaa aaaaggaaat gaagggaaaa gagttcttca aaggtgatgg aactattccg
     4081 tggtgggtta catcagtatc aatatttgcc acattattaa gtccaatatc atttttatca
     4141 ttagcaggaa attcttatgg tggtacttgg attttgtggt ttgcacaatt aggtatgttt
     4201 atagcaatac ctttgacaat aaaatttttc ttacctctat atagtaggtt agaaatagat
     4261 acagcatatg aatacttaga aatgagatac gaaagcaaag gtcttagagt acttggagct
     4321 ttgatgttta taatatatca aataggacgt atgtctatca taatgtatct tccatcaata
     4381 gttctggctg gtcttacagg aatacctgta aatgtactga taatagttat gggtgtaata
     4441 gcaataattt actcatatac aggtggatta aaggctgtct tgtggaccga ttttatacaa
     4501 ggaatggtat taatagtagg tgttacaggg acattgttct atttaatagc aagtataaat
     4561 ggtggttttg gaacagtaat ggatactctt acaactggac acaagttctt agcaagtaat
     4621 gaagttatgt ttgataaaaa tatactatct acaagtgcat ttataatatt tgttggagct
     4681 ggtcttaata ctttttcatc atatgtatcg agtcaagaca tagttcaaag atttactaca
     4741 acaactgata taaagcaact taataaaatg acatatggaa atggtgtttt atcaatggga
     4801 cttgcaacag tattttatct aattggaact tgtttattta tatactatac tcaaaaccca
     4861 gatttagctc aaacagttca acaagaccaa gtatttgcat catatatagc ttatgaatta
     4921 ccagtgggta taacaggaat actacttgca gcgatatatg cagcttctca atcaacttta
     4981 tcgacagggc ttaattcagt tgctacaagt tggactttag atatacaaac tatcataaca
     5041 aaagatataa gtatggaaaa gcaaactaag atagcacaat atatatcatt aggtgtaggt
     5101 atactttcta tagctgttgc aatagtatta gcaaatggag aaataaagtc agcatatgaa
     5161 tggtttaata gttttatggg gctagtacta ggtgttttag caggaatatt tgtacttggt
     5221 gcattttgta aaaaagcgac taaaatggga gcatatgttg gattcatagt atcagcaatt
     5281 ttagttgttt atttaaaata ccgtatgcca gaagtaactt cttgggcata ttctctaata
     5341 acaatcacga cttctgtagt ggtaggtaca atagttagtg caatagaagc taaagttaaa
     5401 gcaaaagtat ctttaccaaa agctgagact actgtttatt atgaaaaata atatttaaat
     5461 gataaattgt cttatagagt gattagataa ataatttagt tactctataa gattgtataa
     5521 tatactttgt atataaaagt ataaaaatag actttataat tatataggga gatagtgctt
     5581 atgatatttg gaaatataaa tcatagtaag acatattcaa cattgcatga agatttactt
     5641 agatgttttg aatatgctaa agataattca ttaatagatt atgaaaaagg tacttataca
     5701 atagatggtg atgatatttt tgtgaatatt gtagagtata aaacttgtga aaaagaagat
     5761 agattctggg aagctcataa gaagtatata gatgttcatt tgatgcttga tggatgtgaa
     5821 cgaatagata taaattttat agaaaattta gaacaaaaaa tatatgaaga aaagggcgat
     5881 ttcttgcctc ttgatggtaa aaataatgga tacatggaac ttagaaaagg tgatttttta
     5941 gtttgttatc cggaagatgc tcatatgaca ggaataaaag ttttagaaaa agaaaatatc
     6001 aaaaaagcta tttttaaggt tttggttaga taatgataat ttatataacc actagggctg
     6061 aaaatagtga tttaactttc agcgattagt gagacttcgt aaattttagt tagattacta
     6121 ttttcagcct tagtttttta tgagaaaatt gaaaataatt aaaagcttaa aattttgtaa
     6181 aggagttaac ttatggaaaa tatagatgta ttcttattaa taggacaaag caatgctaga
     6241 ggattgggaa atccaaaaga gagcatcatt ccaaatgaaa attgttttga atatttgagt
     6301 actgatgaaa ttataaatat gagatgtgag ctagagactt cagaaggtga tggtactata
     6361 gcacctgcat tttcaaatga atggaataag cttactggaa ataaagtttg ttttattcac
     6421 aatgcaaaag atggttcaag aataaaaaac tggaatcatg ataataactg gtttctaaat
     6481 gacactatag aaaaatttaa tacaggctgt actaccttaa gtaaacatta taatatagaa
     6541 aataaatacg ttatttggat tcaaggtgaa agtgatgcaa aatatggtag tgatgtattg
     6601 tactataaag aaagtctaaa aaaaattgcg tataggctta aagaagaatg tatgattgat
     6661 aaaatgtttg taagtttaac tggttattgg cttggagaag atgaatattt tattagaaca
     6721 agacgaattg cagcagcaca ggagtcggct tgtagtgaat gtgatatatt atgtgtagga
     6781 agcaaaattg ctatgcagtt tcatgacaga ggattaacta ttgatgatgt tcattacact
     6841 caagaaggtc ttaatatgtt aggagaggac ttatgtaaaa atatttataa gtatcatata
     6901 accaaagaaa aaagtgtatt aagtgatact atagacttat cagaagcaag gaaatatatc
     6961 tgtgaattgg aaaagataag taaaagattt gtgtaatata aaattttaaa tagagttgtt
     7021 aatttataaa aataatttgt atggagatat gttatgaaaa attacgtttg tatagatgtt
     7081 gggggtactt caataaaata tggttttatt agagaagatg gatttataat tgataaatca
     7141 agtcttgata ctgaagcaaa ggaaaaaggt ggagaaggaa tattagccaa aataaaagat
     7201 atagtaaaaa aatatataga ggaaaatgaa ataagtggta tatgtatatc aactgcaggt
     7261 atggttgacc cagttgaagg taaaatacta tttgcattag aagaattaat tccaaactat
     7321 aagggcatgc aattaaaaaa agaagtagaa aaagagttta atataaaatg tgaagttgaa
     7381 aatgatgtta attgtgctgg acttggtgaa atgtggcttg gagcaggtaa agatgcaaca
     7441 tcttctatat gtttaacaat aggtactgga attggtggat gtattataat aaataataaa
     7501 ctgataaatg ggtttagtaa tagtgctggt gaagtaggat atatgaatgt aaatggaagt
     7561 tcttttcaag aattggcatc tactagcagt ctaattaaaa aagtagcaaa aataaaaaat
     7621 ttgaatgaaa atgacttaaa tggaaaaata atttttgatt tagcaaagaa taatgatgaa
     7681 gattgtttaa aagaactcga taatatgata aaatcattag cagttggaat cgcaaatata
     7741 tgttatgtaa taaatccaga agtggtaatt ttaggtggtg ggattatggc acaagaaaag
     7801 ttcctaaaac caaaaataga caaagcatta aaggaggttc ttatagagag agtttacaaa
     7861 aacactaata tagaatttgc aaagagacaa aatgatgcag gaatgcttgg agcattatat
     7921 aattttttaa acaaaatata aagaatagaa gggtttttaa tgagtatttt agaacaattg
     7981 gaatcaccaa cttttaaagt cacaaaatca gataaattat taatagctta tataaaagaa
     8041 aatatagatg atgtatttta taaaccaata tcacaaatag ctaaagagag caatattggt
     8101 gaagcaacta taactaggtt tgtaaaaaaa atgaatttta atggattaca ggattttaaa
     8161 gttactttag cacaagaaat atcaactatc aataaaaaaa atattataaa taaaaatata
     8221 caaaatgatg agccagcttt ggatacagca aaaaaacttt taagttcaaa tgtaaccact
     8281 ttagagaaca cagtagagat aatcaatagt aaggatgtac atgactgcgc aaggcttata
     8341 ataaatgcaa agaaagttta cttcataggt attggatatt ctggtataat agcacaggat
     8401 tcaaactata aatttatgag aataggtctt aattgtgtaa gttttgacag tagtcataca
     8461 atgataatga tgtcttccat aatggaagaa ggagatttaa taattgcaat ttcacattct
     8521 ggtgaaacag aagagattat aaaaactgta aagcttgcaa gagccaataa tgctaaaatt
     8581 atatcaatta ctgaaaataa aaattctgaa ttaaaggata tttcagatgt acacttatct
     8641 tatgtatcgg gagaaacagt actggaaact ggttcaatat cttctaaatt agcacaattt
     8701 ttcattattg atttagtata tacacaggta gtaaaagaac tttctaatga agctattgaa
     8761 aggaaaatta aaactacaaa tgctataaaa ttgtttaaaa atgaataaaa taagaaattt
     8821 gtaatgtttt atagagctct cttaaaaatt taggaatgga gagatattac atgtctaaat
     8881 taaaagatta taatattgat aaagtaataa tttttaataa tttaagtact aaaacgaaga
     8941 atcaacttaa agaaaaagtt aaactaaaaa gattacaaaa aaaagaaatt ttattttatg
     9001 aaaaagatag cttagacagt gtttatatac ttttagaagg aaaggtatct atttttaaga
     9061 taagtgaaaa tggagagaga aaagtaattt tcatattaaa tagtggggaa gtcataaatg
     9121 atattacact tgacgcttct aaaaactcta ctgttggctg tgaagcattt gaaaattcaa
     9181 ttgtagctta ttatagtatt gaagaatttt tagaaattat gcaaaatgat tttcaactta
     9241 caaaggatat tatttcttat atggaaagaa gaataagacg tttatataga cagttgaaaa
     9301 attctatatc cataaaagta gataaaaaac ttgcagcaaa attatatagg ttaagtagag
     9361 aatttggagt ttgctgtgga gaatggactt tactaaatgt aaatattaca gttacatatt
     9421 tagctgatat gcttggctgt aaaagggaga ctgtttccag aatgataaag gtattgcaaa
     9481 aagaagaact tattaaaatt gacaataaag gtttttatgt taaagaaact gaattatcaa
     9541 ggtattttaa aaataattaa ttatgattaa gtaagagtat tattagaatt ttaattaaat
     9601 aa
//
LOCUS       DAMJZE010000152         9402 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJZE010000152 DAMJZE010000000
VERSION     DAMJZE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.156"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            151..780
                     /locus_tag="PC541_003016"
     CDS             151..780
                     /locus_tag="PC541_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840460.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            781..1563
                     /locus_tag="PC541_003017"
     CDS             781..1563
                     /locus_tag="PC541_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840461.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1556..2434
                     /locus_tag="PC541_003018"
     CDS             1556..2434
                     /locus_tag="PC541_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840462.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2467..3426
                     /locus_tag="PC541_003019"
     CDS             2467..3426
                     /locus_tag="PC541_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2840463.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3741..4433
                     /locus_tag="PC541_003020"
     CDS             3741..4433
                     /locus_tag="PC541_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840464.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4443..5447
                     /locus_tag="PC541_003021"
     CDS             4443..5447
                     /locus_tag="PC541_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2840465.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5715..6566
                     /locus_tag="PC541_003022"
     CDS             5715..6566
                     /locus_tag="PC541_003022"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2840466.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6670..7416
                     /locus_tag="PC541_003023"
     CDS             6670..7416
                     /locus_tag="PC541_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840467.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7416..9332
                     /locus_tag="PC541_003024"
     CDS             7416..9332
                     /locus_tag="PC541_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840468.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3654 a          883 c         1664 g         3201 t
ORIGIN      
        1 acatatacta tttaatattt catttaaaat attaaaaaat gtagataaaa attgttttat
       61 taagaaaaaa ttaatatatt gacaaaattt aagtgctcat ttaaactatt aattgataat
      121 aatattcaat attaaataat aggaggcgtt atgaataaat atcttctaat aaatccagtg
      181 gcagaaagaa tgtatggaga aaagtttcac cttattaaag caaatttgat aaaaaagggg
      241 tacataatag cagaatgtga accacagtta gaatatgtaa aaaagcagta caaagagtat
      301 acaaaaaaga ctgaaaatac aatgttggat tgtagatgtc cagaaagtat aagtctttta
      361 aaaagaaata atttgataaa tgattttgaa gtacctatga tagaaccaat attagtaagg
      421 acttgtaggg ttttatacga taaatatata aaaagtaaag atgatatgtt ggtagtaacc
      481 tgtccatgta cacaattaag agactttgca agtgaaaagt ttaaggataa gggtaatgct
      541 atgttttata catggaaaga gtttgctgaa caagaaggtg taaaatcact tggaaaaata
      601 gatgaatcac caattccatt aggatatttc aaagatactt ttgaaaaagt tcttgaagta
      661 tcaagtgaag acgaaatatt aagtgaaata caaaaaataa gaaatggctt tgaagataag
      721 tatgacatag tagaaatgtt ttattgtaaa gatggttgta ataatggaga tggtttgtaa
      781 atggtcaata aatttaataa taagttttta aggctagtaa agagtgtttt gatatttttt
      841 ataattcttt tattatggaa aattacaaat tatcttggta tttggagtga ttatatatta
      901 ccatcacctg aaaaggtata tagtacattt ttaaatatga taagtgatgg ttcaatattt
      961 attaatgtat atgcaagtat gaaaagagtt ttgataggtt ttgcaataag tactgctata
     1021 ggtgtgcctt tagggatatt ctttggaatt tatagtggag tatacgaata ctttaaatct
     1081 ctaataaatt ttttaaggaa tacacctccc cttgctttaa taccaatgct tatattatgg
     1141 tttggtatag gagaggaatc aaaaattata attatagttt tagcatcctt tttcccaatc
     1201 tttacaagca cattgaaagg gattaaaaat tgtgactcaa aactgattga agtgggtaga
     1261 gtatttgagt tttcaaagct tcagataata tttaaaataa taataccaaa tgccattctt
     1321 gatattgctg ttgggttaaa gttagctctt ggatatagct ttagagccat tattggggca
     1381 gaacttgtag cagcttcatc tggtcttgga tacttgatat ctgatggaaa agaaatgtct
     1441 agaacagatg ttgttattgt aggtataatc gtaattggac ttttaggaat aattacagat
     1501 tacatatttt cgattattgt gaagaaggta agtaagggaa agatggtgga agcatatgaa
     1561 taatttagta tgtgactctg gatatataat tagaaattta tataaaagtt ataaagttga
     1621 caatgaagaa catgtagttc ttagtgatat ctctttaaac atagatagaa accatataac
     1681 agtaatttta ggtgaaagtg gttgtggaaa gactaccttg cttagagcaa ttgcatcact
     1741 tgaaggtata aatagtggtg aaattaggtt tattaatgat aataaagagt atagacctaa
     1801 tgttggtttt gtttttcagg agagcagatt aatgccttgg ttaaacgtaa gtgaaaatat
     1861 agcttttcat aatcaaagaa aaaatactat tttaaacaag gtgcttagaa aatttaaaca
     1921 caataaaaga attagcagta aaattataaa tgataatttt agaagcttag aaacaaatga
     1981 taatttgaac aaatcaaata aatataatag gaaaagtaat attgatgtag aaaaatatct
     2041 taagatgatg aacttgcaga aatttaaaaa ctcttatcca aatgaattgt caggaggtat
     2101 ggcgcagaga gtaagcatag ctagagctct ttctttcaat ccagatatgt tgcttatgga
     2161 cgagcctttt tcggcattag attattttac aaggattgat atgcaaaatg aagttataag
     2221 aatacataag tcaactaata aaggcgtgat atttgtaact catgatattg atgaagcgtt
     2281 aaagataggc aagaaaatag ttgttttcac agacgaaaga aaagtaaaag aatttaatat
     2341 agaagatggt tataatagag atttaacttc agattactat attaaattaa aaaaagatat
     2401 tttaatgaca atgaaatcaa ataaaggcat ttaaagaaat taataatatt ttaaggagta
     2461 aagtaaatga aattaaaaaa gctattgata ggtataacaa tattaacact tgcaacagca
     2521 tctttagctg gatgtgctaa aaaaaataat ggagaaaaac tttcagaaat aaatttaact
     2581 tatgtaaaat caccattaaa tgttccttct ataattcaaa aacaggatga tttatttgga
     2641 aaggaattta aaaaggataa tataaaagtt aatttccacg aaattactac aggtccagag
     2701 caaacacaag ctttagctgc tggagaaata gacttcttac atgcactagg tggtacatca
     2761 gctttaatag ctgcatctaa tggagtagag ttaaagatat taaacactta tagcaggtca
     2821 ccaaaaggat ttatgatttt gactaataat aattcaataa aatcagcagc agatttagta
     2881 ggtaagaagg tggcaggccc aaaagggact atattacatc aggtattgat ttcagcactt
     2941 gataaagaag gattaagtat ggatgatgtg gaatttgtta atatgggtat tccagaggca
     3001 tcagcagctc tttctgatgg cagcgtagat gctgcattga ttgctggtcc agcagctctt
     3061 aaagctatga agtcaggtag taaacttgta gcaaatggtg aaggtttagt agatggtata
     3121 atagtaactg ctgttagcac agactttgca gagaaacatc cagaaatagt tgaaagattt
     3181 atgaaggttg aaaaggaaac tttagaatat gtaaataata attttgatga agctatggaa
     3241 aaagtagcta aagaagttga tttaagtctt gaagaaacta aagaattata tgcttggtat
     3301 gattttagtt tagatataac agataaagac atatcttcgc ttgaagatac acaagacttc
     3361 ttaataaaaa ataagttaca agagaaaaaa gtgaacataa aagaattaat ctataatgct
     3421 aagtagaaac tatgataaaa taatactaag taagaaatat agtaaaagtt taagttacct
     3481 tattaaggta actttttcta ttttgaatta gtaatttcaa tttattttag aatgattagt
     3541 tgataataat gattaattaa tcataaaaag atactttttt atagagtttt attaagatgt
     3601 ctaataatat ggttatactt atgaagtcag taattgtgaa gttagtaata tgattaaatt
     3661 tatatattgt aaaaagatta taatatatta aaattggact gttaatatga tgagtaaata
     3721 ggtatattag gaggaattat atgagttata aagtatatat tgtggaagat gatatatcaa
     3781 taagtacatt acttaaagat tatattgaga aatatggatt tgaagtgatt gttgaagaaa
     3841 actttgatga tataatgatt acatttgaaa gttttaagcc acacttagtt ctattagatg
     3901 taaatttacc taaatttgat ggtttctact ggtgtagatg tattagacaa cagtcgaatt
     3961 tacctataat ttttatatca gctagagatg gtaatgtaga ccaaattaga gctttagaaa
     4021 gtggagcaga tgattatata acaaaacctt tttactatga tgtagtaatg gcaaaaataa
     4081 agagtcatat aagaagggta tatggtgaat attcacctaa aatagacgaa agaatagtgg
     4141 agctagatgg attaaagttt tatccagaga ggttagaact agaattaaat ggtgagaatc
     4201 taattataac taaaaaagaa ggtatactga ttgaatgtct tatcaagaga tacccaaaag
     4261 tagtaagtag agattatctt ttagaaaaga tatgggatga tattgagttt gtagaggaaa
     4321 atactttaaa tgtaaatgtt agcaggataa ggaaaagatt tgaggaatta ggtatagata
     4381 atgctgttca gactgtccga gggttaggat ataaattaaa taagaattgg taaggagaaa
     4441 gtatgaagtt atttttaaaa gattataaag gttatatttt tatatattat ataggcatag
     4501 ttatgacaat tatttactgt aatcttatga agtttattag ttttggagag agtttatata
     4561 ttcttttatt tagttctttt atattgttat gttttttaat atataagtat tataaaaata
     4621 gagaagtata taatctgttt gaaaaaggag tacattcttt ggaggagtca tttttgtatt
     4681 taggaagctc attcttgggg agaaatatat cagttatttt aaaacaacaa cataaattgt
     4741 atcaatcaat gatacaagag cataaaaaga tacatagtga acatttaact ttcatcaacc
     4801 aatggattca tcagatgaaa acaccaatat cagttataag ccttcagctt caagattatg
     4861 agggagaaga agtaactgaa aatataagaa aagaaataga tagattaaat aaagggttag
     4921 atatggctat gcattttgca agggcagata attttcagaa agactttata gttgaaaaag
     4981 ccaaattaaa tgaaattgta tctagtattg taaatgaaga aaaaaggtta tttataaaaa
     5041 gtggaatgat tcctaagatt caagtagaca aatttactta tgtttataca gatgtaaaat
     5101 ggatgaaatt tataataggt caattaatta caaatggagt gaaatattct aaagattata
     5161 gtaactacct aactataaaa tctgaagaaa ataataaata tgtaatttta agtataatag
     5221 atgaaggaat tggaattgtt tctaaagatt taaaaagggt atttgaccca ttttttacag
     5281 gagaaaatgg tcgtaagttt ggagaatcta caggtatggg tctttatcta gtaaaaaagg
     5341 tatgcgatga tttaggtcat aaagtaagta taaaatcaga agtaggaaaa ggtacagaag
     5401 tttcaatcta ctttaataaa gatgtaaata gtttaataaa ggtgtaaata gtttgataaa
     5461 gatgtaaatt atctgttaca atagggtttt tagttaattg tactttttta ttatacaatt
     5521 tattaaatga aatattcata gttaaataat caaaatttaa gagcgtttat ttgcaaacct
     5581 tacatagatg taaggtttgt gtaatgtttg cagatagtta aattttctaa tctaaattac
     5641 aattaatata agaaataaca taagagaaat aacataaaaa aagaaatttt gtaaaaaagg
     5701 aaggatgttt attgatgagt ttagatgtta tttttatatt gaaatcagtc ataatagcta
     5761 tagtagaggg gcttacagaa tttattccag tttcatctac aggtcatatg attttggttg
     5821 gtaatttaat agattttaag gggcaatttg ctgaaatgtt tgaggttgta atacaacttg
     5881 gggctatatt agcagtagta gtactttatt ggaaaaagat aaaagatagc gttatagaat
     5941 tttttaaatt tatatttact ggtggaaaag aaggaaagat tggttttaga tttggaatga
     6001 atgtaattat aggttgtata ccatttgcga ttataggagt tctattttac gataatataa
     6061 aatcattgtt taatttacaa tctgtaatta taggatttat agtgggtggg attttattat
     6121 tagtagtaga aactttgttt agaaaaaaga atcattccac agataatata gataaaatta
     6181 ctccaataca agctttaaag gttgggacat tacaagtttt gtctgcatgg ccaggtatgt
     6241 caaggagtgc atctactatt atgggtggat ggatagcagg acttaattct cctacagcag
     6301 ctgagttttc tttcttttta gcagtaccag ctatggtggc ttcttcagga aaagatttat
     6361 ttgaatttga ttattcaata atgacaccaa cattgtggat tgctttggta gttgggttta
     6421 tagtagcttt tattgtatca ataatagtta tggaaaaatt cgtgaatttt ttaaagaaaa
     6481 aaccaatgag agtgtttgct gtatacagaa taataatggg tgtagtactt gcagtattag
     6541 cttttactaa cataattagt gtatagaaaa aatagattta tcatctataa aaattaaact
     6601 aagataaatt ataattaaca attatatata tgttatttca aaaattaaat taggaaggat
     6661 agttaaataa tgaaaatttt aactgtaaat aatttaagta aagtatatgg taaaaaaata
     6721 attttcaacg cattaaatga cataaatttt agtatagaag atggagaatt tgtaggaatt
     6781 atgggtccat ctggtagtgg aaaaactact cttttaaata tgatttctac tattgacaag
     6841 ccaactacag gaactatgga attaaagggc aagaatcctt tattgttaag aggtgaggaa
     6901 cttgcattac ttagaagaag agagttaggt tttgtatttc aggattttaa tttattggat
     6961 acattaacta taggagaaaa tatagtattg ccattgacat tagataaggt cagtgtaaaa
     7021 gagcaagatg agagattgaa tgaagtatct acaatattag gtataaaaga tttattagga
     7081 aaaagaactt ttgaagtatc aggtggtcaa gctcagagga ctgctattgc tagagcattg
     7141 attaacaatc catcaatact tttagcagat gaaccaacag gtaatttaga ttcaaagtca
     7201 tcaaaggttg taatggagct atttcaaaag ataaataaag aaaataaagt tactactatg
     7261 atggtaactc atgacccatt agcagcgagc tactgtagta gaatcttatt cataaaagat
     7321 gggtctatat ataatgaaat atataagggt agtagtagag agcagtttta tcaagagata
     7381 atggatgtac ttacattatt gggaggcgat aactaatgaa tataaaacag tttgctatta
     7441 acaatatttc aagaaatatt aaagcgtatt taggatactt agcaagtatt gttatatcat
     7501 cttcactgct attttctttt attatgttta caagtcatcc agatttagat gtatctcaat
     7561 ttcctgatta tttaaaagaa ggtctcaaga tgagtaagat aattgcatat ctattcttat
     7621 tcttctttgt gttttattca gttagtgtgt ttcttaaaag tagatataaa gaatttggaa
     7681 tactttatat tacaggtata tcaaagcgcc aagtaatgaa attaatcttt atagaaaata
     7741 tgataataaa tatagtatct tctgtaattg gaataataat aggcttaatc tttgcaaaga
     7801 tatttttagt gattatgtct acttttttag aattatcacc attaaaattc tatataccat
     7861 ttaatgcgat attgtctact ttaatctatt ttatgatttt agctgtatta acatcaatat
     7921 ttacttcttt tatagttaaa gaaaatcagg tgttgaagtt acttaaaggt acaaaaactc
     7981 caaaaccaga gcctaaaaca tcactaatgc ttgctatatt aagtatttgt ttgtttataa
     8041 tagcgtatta taatgctatt acctcaaccg accaatatac aacatatctg cgcttagttc
     8101 cagttacatc ccttactata gtagcaactt acttgttctt ttctcaattt agtgtatttt
     8161 ttatgaagaa gctaaaacta aacaagagat tttatagaaa aaatacaaat atgctttgga
     8221 tttcgaactt gatttataag gtaaaggata atgctagaat atttttcttg ataactataa
     8281 cttctgcagt agcatttact gctataggta cagtttattc cttttggaaa gatgtagagc
     8341 gtcaaattaa tttaatatat cctaacacaa tatattattc aacaatgaca ttgcataatg
     8401 accctaaaaa gcctgatagc aaagaaaaag acaaggaaag aatgatgttt atagaaaata
     8461 atctaaaaaa agagaatgta gactatacaa ggactaatgg tgaatttaaa actgtatttc
     8521 ccaagaaaag tgattttaat gtcagaatta tgaaagaatc taagtactta gaaattacag
     8581 aaactatagg ggttaagtct ataagttttg aagataatga atgtatatca ttattatcat
     8641 caagtttacc aggaaataat aacgtcaaag aaaatattat tgtagggaat aggggtttaa
     8701 aggtggcaaa acaagtggaa gaatgtgtta tgccagcata ttacaaaaat atgtatgtgg
     8761 taaaggataa tttttatgat agtatagaat caaaatttat agtggataga tttactgcat
     8821 ttgaagtaga ggattcaagt aaaactatag agatatgcag agaatttgaa aataaattta
     8881 ataatgagtc agggatgcaa ccatatttgt ttttctctaa agaattaatg ataggaactg
     8941 ggaaattaat gtattctaca tttatatttt tagcaatatt tataggttta attttctttg
     9001 ttactacaag tagtttttta tataataaat tttatatgga ttgccaagtt gataagaaaa
     9061 agtatgaaca gttaaataaa ttgggtatga catataatga gattaaaaaa gcttcaacaa
     9121 ttgagattgg aatagtattt ttgtttcctt atgtagtggc tgttatacat tcaatatttg
     9181 cacttactgc acttaaaaat tcttttgata tagaagtagc atcatcagct gttttggtaa
     9241 tgggaagctt gttcttggtt caaatagttt atttttttat tgtaagaaat aattatttaa
     9301 aagagataag gttaaattta gtcaattttt agttactatt attaaatgat aataaaaagg
     9361 gtttaaaatt aattaatatt actggaatta tagatgttta tt
//
LOCUS       DAMJZE010000153         9255 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJZE010000153 DAMJZE010000000
VERSION     DAMJZE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.157"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            288..1304
                     /gene="gap"
                     /locus_tag="PC541_003025"
     CDS             288..1304
                     /gene="gap"
                     /locus_tag="PC541_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2840469.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1699..2868
                     /locus_tag="PC541_003026"
     CDS             1699..2868
                     /locus_tag="PC541_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840470.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3248..7501
                     /locus_tag="PC541_003027"
     CDS             3248..7501
                     /locus_tag="PC541_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2840471.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7650..8144
                     /locus_tag="PC541_003028"
     CDS             7650..8144
                     /locus_tag="PC541_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2840472.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8260..8637
                     /locus_tag="PC541_003029"
     CDS             8260..8637
                     /locus_tag="PC541_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2840473.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            8843..>9255
                     /locus_tag="PC541_003030"
     CDS             8843..>9255
                     /locus_tag="PC541_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2840474.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKD"
BASE COUNT         3752 a          960 c         1663 g         2880 t
ORIGIN      
        1 ttgaagatat attattttta aaaataagta tagtttaaaa attcaaaaaa aagatggcaa
       61 aatatgtccc aagggacatt gagtaaaata aatgatttca gcttatttac actaaaaagt
      121 acatactaaa tgaggggtaa aataaagaaa aatgatatac taattgtctt ttgataataa
      181 aaaaacatgg aatttgatta tttaatcaag aaaaagtatt gaacaaatga tttatttagt
      241 gtacaataat catgtaaaaa catagtaatg gagggagaca tttcataatg aaaattaagg
      301 taggaatgaa cggatttgga agaataggaa gagctgtatt aagaatagct caagaagaat
      361 taggagataa tatagaaatt gtagccataa atgcaagagc aacaacagaa tctttggcac
      421 atttatttac atatgattca tgctatggaa cttttagagg tgaagtagag gcaaaggatg
      481 aagacactct agtagtaaac tctaaagaga taaaaatatt aagatataat gacccagaag
      541 aattaccatg gaaagaactg ggggttgata tagtaataga atcaacagga ttatttacac
      601 aaagagaaaa ggcagaaaag catattaaag caggagcaaa aaaagtaatt ataacagctc
      661 caggaaaaaa tgaagatata actatagtta tcggagttaa tgagaaagaa tatgataatg
      721 aaaagcacaa tataatttca aatgcatctt gtactacaaa ttgtctagct ccttttgcta
      781 aagtattgga cgaaaaattt ggtatagtaa aagggttaat gactacagta cattcatata
      841 ctaacgatca aagaatatta gataaaagcc ataaagattt gagaagagca agagcagcgg
      901 ctgaatctat aatacctacc actacaggag cagcaaaagc tgtgtcaaga gtactaccac
      961 aattagaagg aaaattaaat ggtttttcac ttagagtacc aacaccaaca gtttcacttg
     1021 ttgatttagt gtgcgaactt aaagaaaacg ttacatctga gcaagttaac tctgtgttaa
     1081 aagatgctgc tgaaggagaa ttaaaaggtg ttttaggata ttgtgacaag ccacttgttt
     1141 ctatagatta taaaggagat tcaagatcat ctattataga cgctctatcc acaatggtta
     1201 tagaagacaa tatggttaag gtagtttctt ggtatgataa tgaatgggga tattcttcaa
     1261 gaactgttga cttagtaaaa tatgtagctg aaaaattaaa ataatatata tttcttaaag
     1321 caaattttaa aaaaattttt taaaaaaatt atataaaact aaaaaaaaag attatgaaaa
     1381 atattgaagg aaaaattttt tcataatctt ttttctttca tgcaaaaaga tacaagttac
     1441 aacttatatt tgcatattag gtcgttagag aagtatatat atatttagta aggaatcata
     1501 ctttttataa aatgaaagat aagatgtcga aaacttgcat aaatctgaaa aatttttatt
     1561 attaaaatac tgatttccat tttaaaaatg ataaaaaaat gacaaataaa gttgcaaata
     1621 gtaatactta taagtataat taaaaaagag aaaaggtttt gttaaacttt ataacaaaaa
     1681 aagaagggat gattcaaaat ggagaagtta acagacagga gacaggttaa ggcagtaagc
     1741 atggaaacct tcattttcct aatattacta gtagttggtt ttgggtatgt tggtagtatt
     1801 atgggagcag gaatgatgtt caaagtaata atgagtacag cacatgcttt actattggag
     1861 acagttttct taattatggc tatggcagtt cttgcaggcg cattaagcgc actactttca
     1921 gagtttggag taatagcgtt aattaataaa attttcgctg tatttatgaa gcctttatat
     1981 ggactacctg gtgctagtat agcaggagct ataacaactt atttatcaga taatccagct
     2041 attatacctt ttgctaaaga taaaacattc acacagtatt ttaaacaata ccaagttcca
     2101 gcattatgta acttaggaac agcctttggt atgggactta tcttaacaac atttatgatt
     2161 tctcaaggta cagaatatgt acttccagca ttaattggta atttaggtgc aattataggt
     2221 agtgtcataa gtgttagaat aatgttgaca tttactaaga agttttataa atacagtccg
     2281 gaagaagata aagcaacagg aacattagag aaaaaggatg aatttagaga aatcagagaa
     2341 ggaaatgtat ttcaaagagc tttagatgca attcttgaag gtggtaaaat gggggtagat
     2401 atgggaatgg ctataattcc tggagtccta gtagtatgta cattggtgat gttattaact
     2461 tttggaccat ctacagaccc tgttacaggt caagaagttt atacaggagc tgcatatgaa
     2521 ggaataaaat tacttccagt tattggagat aaacttggat ttatattaga accattattt
     2581 ggatttacat caccagaggc aatagcattc cctataactt cattgggagc tgttggcgca
     2641 gcaatgtctc tagttccaga gtttataaag tcaggagcta taactcctaa tgatatagca
     2701 gtatttacag ctatgggtat gtgttggagt ggatatttaa gtacacatat tggtatgatg
     2761 gatgcattaa atgctagaca gttggcaggt aaagctattc tttctcatac aataggtggt
     2821 ttatgtgcag gagcagctgc tcactttata tttacactcg taggataaat gtaatcagct
     2881 aagataaaaa ataatataca aaaaatggtt gttgatatgt aataagaatt tacatatcaa
     2941 caaccatttt tttctgacta aagataattt ataatacata aacattaagt ggatattaga
     3001 gttaagttat atttatgtaa aaagtaacaa aaatctgtag aaatttatga caaaatttag
     3061 tacactaact atatgttctt atttaaaatt taaggttaga attaggaatt acctagtaga
     3121 gtaagctttg attaataaaa gaatcataaa ataaaaatat tagtcataaa gattataaat
     3181 aacttactta aaataaagcc atagaactta caaaaattaa aaaagcatat aaatatggag
     3241 gggtacaatg gatgaattat ttcatatagg aattgatgta ggttctacaa cggtgaaggt
     3301 tgtggtactt aataatagta ataatatagt tcataaagaa tatagaagac attattctga
     3361 tgtaaaaaaa tcagttaaag aagtactaaa tggtatatat gaaaaattgg gagatataaa
     3421 tactaccatt ataataactg gttcaggtgg aattggaata tcaaaaaaat taggtgtaaa
     3481 atttgttcag gaagttatat ctagtacaaa atcaatagag tattttcatc ctgagactga
     3541 tgttgtcata gaacttgggg gtgaagatgc aaagataaca tatctaagtg gaggaataga
     3601 ccaaagaatg aatggaatat gtgcaggtgg cactggagca ttcatagacc agatggcatc
     3661 actcttaaag acagatgcca gtggattaaa tgaacttgct aaagggtata atgttatata
     3721 tcccatagct tcaagatgtg gtgtttttgc taaaacagat attcaacctc ttataaatga
     3781 tggagccaat cagactgata tagcaatgag tattttcaat gcagttgtcg tacaaactgt
     3841 gagtgtcctt tcttgtggaa gaaaaataga aggaaatgtt gcgtttttgg gtggtccatt
     3901 gtacttttta tctgaactta gagaagcatt taaaagagta ttagatttaa aagatgaaaa
     3961 cataatcttc ccagaaaatg cacaacttta tattgctatt ggagcaggat tattatcagt
     4021 agatgaagat aatatacaac taaaatctct aatagaaaaa ttggatagta taaagaatat
     4081 tgaagatgga gaagtaaatt tattagaacc tttatttaaa gataaaaatg agtatgaaga
     4141 attttcaaga agacatgaaa aagaaaaaat aaattatgtg gacataaatt caataaaaag
     4201 caattgttat ttaggaattg atgcaggctc aactacaact aaagcagcac ttattgatga
     4261 ggatggaaga cttgtatatt cttattataa tagcaatgaa ggaaatccat taaaaacaac
     4321 cattaaagtt ataaatgaag tatatgatat attaccaaaa aatattaaaa tattgagttc
     4381 aactgtaact ggatatggag aaggtcttat taaaaaagct cttaaaatcg ataatggaga
     4441 gatagagact atagcacatt acaaagctgc taaatttttc aacaaagatg tagattttat
     4501 acttgatatt ggtggtcaag atatgaagtg cctaaaaata aaagatggag taattgataa
     4561 tataatatta aatgaggctt gttcatcagg ttgtggctca tttttagaga catttgcaag
     4621 ttctttatct atgacaatag aagaatttgc gcatgaaggt atttattctc aaaatcctgt
     4681 agatttaggt tcaagatgta ctgtatttat gaattctaga gtaaaacaag ctcaaaaaga
     4741 aggtgcaagt gttggagata tatcagcagg gctatcttat tcagtaataa aaaatgcttt
     4801 atttaaagta ataaagatta gagatttaaa tgagattggt aataatattg ttgtacaagg
     4861 tggaaccttt tataatgatt tggtgcttag aagctttgaa aaactaattg gaaaaaatgt
     4921 aattagacct aatatttcag gaattatggg agcttttggt tcagcactta tagctaaaga
     4981 aaaatatgaa agtggataca aaacatcctt gttatctaag gaagagttga ataatatcaa
     5041 gctagaagca agtgtaacta gatgtaaagg ttgttcaaat cattgtcttc ttacaataaa
     5101 taaattttct gataatgaaa tttttatttc aggtaataga tgtgagaagg gtgaagctat
     5161 ttatggtgat aaaaaaatag gattagaaaa gaaaaaacca ataaatttat ttaagtacaa
     5221 atacaataga atttttagat acaaaccatt ggaaaaagaa gaagcaaaaa atggggaaat
     5281 agggatacca agagtattaa atatgtatga agattatcca ttttggttta cttttttcaa
     5341 tgctatgggc tttagagttg tactttcaga taggtcatca aaacagttat atgaaactgg
     5401 tataactagt attgcatctg aaacagtttg ttatccagga aaattagttc atggacatat
     5461 agaaaattta attgcaaaag gtataaaaaa tatattttat ccaagtgtaa caaatgaaaa
     5521 taaagaggat tcaaatgcag ataattatta taattgtcct gttgtaatat catattctga
     5581 agtaataaaa aacaatgtag agaatataag aaataagaac ataaattata taaatccatt
     5641 tataagtcta aatgataagg aaaaattgaa gaaaagactc tatgatgaat tatcaagaca
     5701 tttttctgat ataaaaataa ctaaagaaga agttaatcat gcagttgata aagctactga
     5761 tgaacagaat ttatttaaaa tggagataca agcagcaggt gaaagagcac ttaaagaaat
     5821 aaaagaaaga aatatgaagg ggattgtgtt atgtggaaga ccatatcata tagacccaga
     5881 aataaatcat ggaatgccag agcttataaa ttcattagat atggcagtgc ttactgaaga
     5941 ctctatatgt catttagcca atgtacaaag accacttaga gttgtagacc aatgggtata
     6001 tcattcaaga ttgtataaag cagctagttt tgtaagagat aaaaactatt tggaacttgt
     6061 tcaacttaat tcatttggct gtggattaga tgcagtaact acagaccagg ttcaagaaat
     6121 acttaatgaa aaatcgaaga tatatactat tataaagatt gacgaaggca acaatttagg
     6181 agcagctaag ataaggatga ggtccttaaa agcagctatg tttgagagag aaagcaaaaa
     6241 tatagatgta aaaaatataa aggtaaacag gatacagtat gctaaaaata atcagataac
     6301 gaacaaacat actatattag caccacaaat gtctccaata catttccaat tccttgaaaa
     6361 agctgttaac ttaagtggtt ataatataga aattctaaaa gatactgata gtagtgtaat
     6421 tgaagaaggg ttaagatatg taaataatga tgcatgttat ccagctataa tagttgtagg
     6481 tcagctaata acagcattaa aatctggcaa atatgattta aataacactt ctgttactat
     6541 aactcagact ggtggaggtt gtagagctac aaattatatt ggattcttaa gaaaggctat
     6601 atacgatgct ggatttaaag atgttcctgt cattgcactt agtgtaaatg gaatagaaga
     6661 tagtggtata atggataata tatcactaaa attaatcaat agactcttta tgtctgttgt
     6721 ttatggtgat ttgctgatga aagtactata tagagttaga ccatatgaaa aagttactgg
     6781 aagtgcaaat gctctatatg aaaaatgggt tgatatatgc aaatcttctc ttgttaaagc
     6841 aaaaatttct gtatttacta gaaatataaa agatatagta aaagaatttg ataatttaga
     6901 gatactagat atcaaaaaac caaaagtagg tttggttgga gaaatattgg tgaaattcca
     6961 tccaattgca aataataatt tagtagatat attagagcga gaaggtgctg aagcagtcgt
     7021 gccagacctt acaaatttct tcttaagttg tgcctttaat actatataca aacacactca
     7081 tttagaagga agtagaaaga gtagaatgat aggagaggca tttatataca taactggaat
     7141 atatcaaaga gtatataaaa aagcacttga taagagtgag agattttatg ctccagccaa
     7201 tataaaaaat gttgctaaaa gtacagaacc agtagtgtca ttaggtaatc aaactggaga
     7261 aggttggctt ttaacaggtg aaatggttga attattaaat gaaggtgtag aaaacattat
     7321 ttgtatgcaa ccttttggat gtttacctaa tcacataatt ggtaagggtt ctataaaaga
     7381 attaaagagg ttgtataaaa atgcaaatat aatacctatt gattatgacc cttctgcaag
     7441 tgaggtaaat caacttaaca ggataaaact tatgctttct aaggctttta aaaatatata
     7501 agatttatat tttgatatgt taaaagttaa tgaatttaaa aatatactct atattaagta
     7561 tctataaaaa agagttatcc tattttttag ggtaactctt tattagttaa aaaattatat
     7621 gtatactgat attaaagggg ggaagtaata tgagtttagt atatgatgtt tataaatcaa
     7681 aattaggaaa tttgtacata ttaagtgaag atggttttat agttagtata tacataggag
     7741 atgaaaaatt taataattta aaaaatgaat gtattaaaaa aggaattgat ataaagagaa
     7801 gtaaggaaga tttaaaggaa gcaataagcc agatagatga ttactttaat ggaaaaagaa
     7861 aagtctttaa tttaaatatt cgtattgaag ggactgaatt tcaaaaatca gtttataggg
     7921 aaatgttaaa aataccttat ggagaaacat tgtgttattc agatatagca agaaatataa
     7981 acaatccaaa atcagtaaga gcaataggac aagcaagtaa aagtaataaa attccaataa
     8041 taataccttg tcatagagta gttggcaaaa aagatattgg tggatatatg gggaatcatt
     8101 ctgatttgaa agagatacta ttaaatttag aaagacagag ttaacttaga aagaaatgtt
     8161 tttattgtta atatttgata taataatagt aaaaagaagt attttttaca aaaacaatga
     8221 agaatttatt ttaatttaca aaaatgaggg ggaattttaa tgtgtgataa agttatagca
     8281 tcaaaatatc atgagaaggg atttaattgt gcagaatcag ttataaaagc atataatgag
     8341 gaatttaata cagatatacc agtttgttta ggaagtggac tgggttcagg gtgtggggtt
     8401 gctagtttat gtggtgcagt aaatgcatca aatataataa ttggatatgt aaaaggcaga
     8461 aaccacgaag atgaaagtac taaagctaaa gtatatgcta aagatttaac tacaacagta
     8521 agaaaagagt atggttcaga gttatgtata gacctaaaaa aagatttagt tgcttgtaga
     8581 gaaataatgg atttcgcata tgatagtcta aaggaaactt taaaaaaaga attataagaa
     8641 aataagctat caaatataaa ttatttgata gcttattttt atagccgata ctaaataaat
     8701 caaatatatt ttagaattat tatttgtaaa taaacagcaa ataattttag gctttttatt
     8761 taatatagtt tttatttata tttatttttg aatatgttat actttgttta aataaatatt
     8821 tgtaatgagg gagatgagaa gattgaagaa tctcaattta cttattaata aattatattc
     8881 aaaaaatcat aatgaagcat ataaaacttt tttattctta gaaaatgaaa gtttaaaatc
     8941 aaatattaca tattgttttt ttgactcatt cctagaaatg attgataacg aaaactcata
     9001 tattcgaaca aggggattgt tattaatctc tgcaaatgct cagtgggata ttgataataa
     9061 aattgaaata aatatagata gtatactaag tcatatcgta gataaaaaac cttctgtatc
     9121 aagaatgttt attaaatcca tacctaatat tacaaagtat aaagaaaatc ttattaatcg
     9181 cataaaaatg gaattaagta atgcagatat aagtatttat aataataata tgaaaccatt
     9241 agtagaaaag gatat
//
LOCUS       DAMJZE010000154         9192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJZE010000154 DAMJZE010000000
VERSION     DAMJZE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.158"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            393..1598
                     /locus_tag="PC541_003031"
     CDS             393..1598
                     /locus_tag="PC541_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2840475.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="PC541_003032"
     CDS             complement(1669..1890)
                     /locus_tag="PC541_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840476.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC541_003033"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC541_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2840477.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="PC541_003034"
     CDS             complement(2643..2993)
                     /locus_tag="PC541_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2840478.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC541_003035"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC541_003035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2840479.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="PC541_003036"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC541_003036"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="PC541_003037"
     tRNA            complement(5068..5159)
                     /locus_tag="PC541_003037"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="PC541_003038"
     CDS             complement(5160..5615)
                     /locus_tag="PC541_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2840480.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC541_003039"
     CDS             complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC541_003039"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2840481.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7333..7538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7666..8547)
                     /locus_tag="PC541_003040"
     CDS             complement(7666..8547)
                     /locus_tag="PC541_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2840482.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8559..9068)
                     /locus_tag="PC541_003041"
     CDS             complement(8559..9068)
                     /locus_tag="PC541_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2840483.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2943 a         1599 c          973 g         3677 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggttc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ttacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtagcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatggtact gtcctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagta
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcctca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataacg tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctactataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 ggtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaataca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 taccaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt ttttaaaatt agttctaaaa taatttttag atattcatac
     5761 aagagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5821 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5881 ctcataatat attctcttca tcaaagcata ctctatatat acaattaata taaatttatt
     5941 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     6001 cctgctgata attttctaat atagcagcca aacatcttcc tattgctaat ccagaaccat
     6061 ttaatgtatg aacatattct gcttttgatt ttttatctct tttaaatctt attcctgctc
     6121 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtaac
     6181 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6241 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6301 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6361 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6421 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6481 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6541 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6601 ttctattggc cataaatgga ggtaagactt ccgtatagcc atgcttagct gtatgagtat
     6661 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatccccta tataaagtaa
     6721 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtaccta
     6781 tatcccaatg cgctttatgc tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6841 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6901 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6961 tcttatctga taactctttc aatcttgcct tttcttctgt tacatctttt ccttctttta
     7021 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     7081 gaagtatttc tcttctttta ttatctaatt ccactacagc atcaaggtca aattctcttt
     7141 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7201 ctaacatatt aattcctcct aataattttg ataatataag ttataattcc aacttaattt
     7261 acaaacatta tacttgtaaa ctcttagttt aaacaaattt taaaaataaa aaaagacctc
     7321 cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt tgccacccta attgactata
     7381 ctaaaatgta taatcctctc agacctataa cgtaggtaaa cgtctagctt taactagaag
     7441 ctctagagta gattcaaaaa ccattgtaat gagttttcac catccactca ttctctgaat
     7501 acaatctgtt ttttactatt ttccttcaca gctttttaac taattgtttt atttgataat
     7561 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7621 tttaattttt tatattaaat tcattttttc tatttaatta aaaccttatc ttttatccat
     7681 tgtatttaga ttacctgtat tattaaataa taatgaagaa ggaacaaatg gaacttttat
     7741 atcattctca tcaaatttat tttttacctt ttccattaca tcaaaatata catcccagta
     7801 atcatcagtt tttacccaag gtctagcaac tatttgcatg gtgttatttc ctatagagtt
     7861 taaacctatt ataggctttg gatcatttaa tattcttttt tcgtttgtaa aaatttcatt
     7921 taatatagac ttaactacat ctatatcact atcatatgaa atattatatg aaaaatctat
     7981 tcttcttttg ttttgatgtg tgtaattaat tatattatta cttgtcagtt gaaaattagg
     8041 tataattata gttttattat ctggcgtctt aagaacagtg ctaaatattt gtatgtcaga
     8101 aactgtacct tctacacctg aaccttgaat ataatcacct atattaaatg gcttaaaaaa
     8161 taaaataatc ataccagagc ctaaatttga taatatttct ttaaaagcta aacctatact
     8221 aaatccagca gctcctaaca cagctacaac tgatgtagtt tgtatcccta taatacctaa
     8281 gcttatcaat gatataatag ataacataga tatatatatt ccatatacaa taaaattatt
     8341 tacaccttta ctcattccat atttgattag aaactttgaa accatccttc ttactatttt
     8401 agatatataa atacctatca caaatacaat tattgcatat ataatatttg gtatattatc
     8461 actaaaatta tcgagcatat tttctaaagt tctcttagtt atattgctat ttgttgaggc
     8521 agtcttagcc aatgataaaa aattcattca actcctcctt atgataatat tccgttgtcc
     8581 tttaactctt ttactatagc ttctatagtt tcttctttta ttgcctcaat tgtatgaata
     8641 tggctacatt ctgtcagaac cgataattgt ttaaattcat ttgtctcaac tttcttcata
     8701 aagttgtcta tatccatttt agaataaata ttaagttctg cagttatctg accatatgtt
     8761 ggatgattta cgatgacatc ttttataata ccacctaaat ccacaattat ctttaattca
     8821 tcataaattt ctttagaatc cttatgattt ttacacttta tttgttttgt aaaatacggt
     8881 tgattatata ttatatatcc ttgaggtgta gctattattt caaatcctct agctcttata
     8941 agggctatat cttgaactat tacttgccta cttacattta atagttcagc tagtgttcca
     9001 ccttttacag gatgcttaga ttcttttaaa atatctatta atttacttct tctttcatta
     9061 gaattcatat tacaccttct ttccaaataa aaactttacc tatactatat aattttatat
     9121 tttatcttat tataaatcaa tattgacttt atattctatt taatcttaac ttttataata
     9181 aataaaaaag ac
//
LOCUS       DAMJZE010000155         9072 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJZE010000155 DAMJZE010000000
VERSION     DAMJZE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.159"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..362
                     /locus_tag="PC541_003042"
     CDS             <1..362
                     /locus_tag="PC541_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2840484.1"
                     /translation="ESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEID
                     LDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNES
                     LILNNLKNLEKQYNSSY"
     gene            375..887
                     /locus_tag="PC541_003043"
     CDS             375..887
                     /locus_tag="PC541_003043"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2840485.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            961..3168
                     /locus_tag="PC541_003044"
     CDS             961..3168
                     /locus_tag="PC541_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2840486.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3193..3642
                     /locus_tag="PC541_003045"
     CDS             3193..3642
                     /locus_tag="PC541_003045"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2840487.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3657..4271
                     /locus_tag="PC541_003046"
     CDS             3657..4271
                     /locus_tag="PC541_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2840488.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4362..5918
                     /locus_tag="PC541_003047"
     CDS             4362..5918
                     /locus_tag="PC541_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2840489.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5908..7170
                     /locus_tag="PC541_003048"
     CDS             5908..7170
                     /locus_tag="PC541_003048"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2840490.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7244..9031
                     /gene="aspS"
                     /locus_tag="PC541_003049"
     CDS             7244..9031
                     /gene="aspS"
                     /locus_tag="PC541_003049"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2840491.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         3670 a          974 c         1761 g         2667 t
ORIGIN      
        1 aagaaagtga aattataaaa tattataata aaacagacct attatattta aagaatgtag
       61 ttagtaatat agttccatca agagatgaat ttataacaat ttataaacaa gcactcataa
      121 aaaaagaaat tgatttggat atggtaaata taagggaaac ttttaatgta attccattaa
      181 agttttttac gatattaaat gtatttagag aattaaattt acttgacttt aatttaaatt
      241 atgaaaaaaa ttctgtactc ataagaatat tgccaaaacc acagaaaaaa ttagacttaa
      301 atgagagttt aatactcaat aatctaaaaa atttagaaaa acaatacaat agcagttatt
      361 aggaggcgtt tcccatggat ttaaaaaact ttataaggaa tattgatgat ttcccaaagc
      421 caggcataga ttttaaagat gtgacaactc tatttaaaga tggagatgca tttaaatatg
      481 cagtggattc tatagttgaa gaattgaaag ataaagatgt agatttagta ataggaccag
      541 aagcaagagg atttttgatg ggtacacctg tagcatatgc tttaggagta ggatttgttc
      601 caattagaaa acctggaaag ttaccaggtg aagttgagag ttatgagtat ggattagagt
      661 atggtacaga tactttagag atacataagg atgcaattaa aaagggtcaa aaagtagcaa
      721 tagtagatga tttattggct actggtggaa ctatggaagc cgctgccaag cttgtagaaa
      781 aattaggtgg agaagtagtt tctatgcaat ttttgattga gctaaaattc ttaaatggga
      841 gagaaaagtt gtctaactat gatgttaatt ctcttataaa atactagtac ttttataata
      901 gttggtataa caccaactat tttcatatat attaaaattt tatacaaaat aggtggttat
      961 atgcatgata aagaattaca agaaataatt gaaaaaataa aagtttatgc acctaatgca
     1021 aattttgaaa tggttgaaaa agcctataat cttgcaaaaa aagcgcatga aggacaatat
     1081 agaaaatctg gggaaccata ctttattcat ccattagcag ttgcaaatat tttaatagag
     1141 atggaattag atattgaaac tataacagca ggtttacttc atgatgttgt agaagataca
     1201 gaatatacct atgaagacat aaaaaaagaa tttggcaaag aagtagcgga tttggttgat
     1261 ggagtaacta aacttggtca aataaaatat cgttcgaaag aagaaactca atctgaaaat
     1321 ttaagaaaaa tgtttttggc aatggcgaag gatataaggg ttatacttat aaaacttgct
     1381 gatagattgc acaacatgag aacattaaaa tacatgcctc cagaaaaagc aaagtataaa
     1441 gcaaaagaga cacttgaaat ttatggtggt atagctcata gattaggaat ttccaagata
     1501 aagtgggaac tagaagacag agcattaaga tttatggacc cagaagggta ttatgattta
     1561 gttagtagag tatcaatgaa gagaagtcaa agagaggatt atattcaagg aattgttgaa
     1621 cttcttaaag ataagtttaa agatttaagt ataccatgtg atgtatatgg tagaccaaag
     1681 catttttaca gtatatatag aaagatgcaa aaaaaacata agacttttga agagattttt
     1741 gatttaacag cagtaaggat attggttgat aatgtaaagg attgctatgc agtacttggt
     1801 atagttcata cattatggag acctattcca ggaagattta aagattatat agcaatgcca
     1861 aaacccaata tgtatcaatc attacacacc actgttgtag gtcctgatgg agaaccttta
     1921 gagatacaaa taagaactca tgagatgcat aatatagctg aaaatggtat agcagcccat
     1981 tggaaatata aagaaggtat ctctagcaaa gaatcaaagg ttgaagaaaa acttcaatgg
     2041 ttgagacaga tgatggagtg ggaaaaagat ttaaaagacc cacaagagtt tatggatgca
     2101 cttaaagaag atgtatttaa tagtcaagtt tatgtattta cgccaaaagg agacgtaatt
     2161 gaattaccag cagaatcaac acctatagat tttgcctata gggttcatac aaatgtaggc
     2221 aataaatgtg taggagctaa aattgatgga agaatagttc ctattgatta taagcttcaa
     2281 aatggtaata ttgtcgaggt acttacatca tctaattcta atggaccaag tagggattgg
     2341 cttaatatag tgaagacccc aaatgcaaaa agtagaatta gacaattttt caagaaggaa
     2401 agaagagaag aaaatgttga ccgtggaaat acaatattag aaagagaatt taagaagcat
     2461 gatataccac aaaaagaccc tcttgttgaa aaatatatgg cattagttgc taagaaattt
     2521 aatcagccaa gtgttgagga cttaattgcc actgtaggtt atggtggtat aatgtcatct
     2581 caagtggtat ctaaggtaaa agacctatat ttaaaagagg ttaaaaaagc taaaaaggaa
     2641 caacagatac aagaagaaga tttaaataaa cacaatataa gtgaaacaga atacagtaaa
     2701 aagagaaaga aaaacgcatc acaaggaatt atagtaaaag ggcttgacaa tatcttagta
     2761 agatttgcta agtgttgtaa tcctcttcca ggagatgaaa ttgtaggata tattactaag
     2821 ggtaggggag tagccataca cagagctgat tgcccaaatg caaacaataa tggtgatttc
     2881 ttccaaaata gattggtgga ggtttcatgg agttcagcag gaaaaggtaa gtttgaagcg
     2941 gaaattcaaa taaaagctat tgatagaaga ggaattgtta atgaaataac tcatattgtt
     3001 gcaatggata aacttagttt aaacggaata aatgcaagaa aaggtaagga tagtattgta
     3061 agtataaact tacttgtaga ggttaatgac acagaggaat taaaaattct aatgaaaaaa
     3121 ttaaaagcaa ttcctggtgt agaaagtatt tatagaatga caaattagag ttaaaataat
     3181 agggaggaaa ctatgagagc agtagtgcaa agagtatctt catctaaagt tacagtagat
     3241 gaaaacacta taggtcaaat aaataagggg ttactagtat tgttaggagt gactcatgat
     3301 gataaatcaa gtgatgtaga ttatatgatt gataaaatat tgaatctgag aatttttgaa
     3361 gatgaaaatg acaagatgaa tttatctcta atggatatag gtggagagct tttagtagta
     3421 tctcaattta ctttatatgg agattgcaga aaaggaagaa gacctggatt tagtaatgca
     3481 gctaggccag aacttgcaaa taacctttat gaagaatttg taaaaaaagc aaaagataaa
     3541 ggtgtgacag ttggtacagg tcaatttgca gctcatatga tggtagagtt aactaatgat
     3601 ggtccagtaa caatactatt agactcaagt aaatcttttt aagggggaaa actttaatga
     3661 ttataaaaaa atttgtagat agttattttg gagtaaatac ttatgtatta ggtgatgaaa
     3721 agactaaaaa atgtgcagtt atagacccag gtggaagttt agtagagaca ttaagttttg
     3781 taaaagaaaa tgaattaaat attgaatata ttatccttac tcatgggcat ggagaccata
     3841 taggatatgt taaggatatt aaagaaaaaa ctggtgctaa agtagttgca catgttgatg
     3901 aaaaagaatt attaaatgac aaaaataaaa atctaagtta tacaatgaga tgtggagcgc
     3961 aagagtttga tgcagatatc tatgtaaatg ataaagacaa gcttgaactt ggtgaattaa
     4021 aaatgacttt cttgcatact ccaggacata ctcaaggatg tatgtgtata agagttgaag
     4081 atgaaatgtt cacaggagat actttgtttg ctggaagtat tggtagaact gatttatata
     4141 gtggagactt taaccaaatg gaaaaatcac ttaagaaatt atgtaaatat gaagatgctg
     4201 ttagagtata tcctggacat ggaccaacca gtactctagg ggttgaaaag aagacaaatc
     4261 cttacatgta attttatatt agtatttaaa aaagtataaa atatttaata actatattag
     4321 tgagggggtt cttctactgt taaatagaag gagttaagtt gatgttgtat gtttttttaa
     4381 aaggacatga ttataagtat gaagtagcag aacttataaa attgtttact agtgaattta
     4441 agtttaaaga ctctaatgct aacaataata tagagtcaaa gcatttaata aatagattga
     4501 tttatgagaa tggtgtatta ttctcaagta ctgaatacta tgaaaatgga aacttaaagt
     4561 acgagtcaat acagaatata gaaaatatga atcttgaatt tttagatgga tttgatagca
     4621 gcaaaacgat tgaagaattt cttaatagcc tagatagtga aagtaagagg aactttaaaa
     4681 agctgtgtaa agaaaatatc aaaaaaagca tgtttttagt gcttaaacaa gtatttaatt
     4741 cttatgtacc atggggaatt ttgactggta ttagaccagt caaaattgtc cacaatttaa
     4801 tggataaaaa gctagatgat aatagcataa gaaccattct aaaagataat tattttataa
     4861 tagatgagaa aatagattta gctcttgaaa tagctaaaag agagcgatta tttatgtatc
     4921 ctatagataa aaataaaatt tctctttatg taagtattcc attttgtcca actagatgtg
     4981 tatattgttc atttccttca aattcattaa aacaatttgg acatttaaaa agagaatatg
     5041 tttataaatt gattgaagaa attaaggggt ttgccaaagt tattaaggat actaagaaag
     5101 aagttgaaac tctatatatt ggtggtggaa ctccaactac acttgatgag gaagaattgg
     5161 atttgcttat aaattcattg ttcaatgaat tagatttaag taaaattaaa gaatttactg
     5221 tagaagcagg tagacctgac actattacag aacaaaaact aagagtgtta aaaaaacaca
     5281 atgttagtag aattagtata aatcctcaaa ctatgaatga tgataccttg gtaaagattg
     5341 gaagagagca tagagtatct gatttagtag attgctttaa tatggctcgt agaatgggat
     5401 ttgataatat aaatatggat ataattttag gtcttataga tgaagactta aatatggtaa
     5461 agaacacatt ggaggagata aaaaaacttt ctcctgaaag ccttacagtt catacattag
     5521 ccataaagag agcatctaac ttaaatataa atatggatag gtataaagaa catctaacac
     5581 aatatgaaga aatggtaaaa atgatagatt tatcaatgaa atatgcaaaa gatatgggat
     5641 taaatccata ttatatgtac agacaaaaac atatgttggg aaatttagaa aatataggtt
     5701 atgctaaaga aggatatgag tgcatttata atatacagat tatggaagaa aaacaaagta
     5761 actatgcatt aggtgcaggt gctatatcga aatttgtcta tgtagatgaa gatagaattg
     5821 aaagagtaga aaatgtaaaa aatgttgaac aatacattga aagagtagat gaaatgataa
     5881 aaaggaagaa agaagaggta tataaaaatg ttgactaaag ctcctagagg aactaaagac
     5941 ataactccaa aagaggctta caaatggcgt tatgtagaaa ataaatttag agaaatatgt
     6001 gctttatatg gatatgaaga aatggtaaca cctatatttg aacatacaga acttttcaag
     6061 agaagtgttg gagatactac agatatagtt caaaaagaaa tgtattcttt taaagataaa
     6121 ggtgatagag aaattaccct aaagccagaa ggtacagcag gtgtagtaag agcatttata
     6181 gaaaataaat tatatgcaga tactcaacca acaaaattat tttatgtgac accttgtttt
     6241 agatatgaaa gaccacaagc tggtagacaa agacaatttc accaatttgg aatagaagca
     6301 cttggaagtg atactccttc aatggatgct gaaataatcg cactagctgt tcaatttttt
     6361 aatgaagttg gattaaatga tttggtagtg agtataaatt cagttggctg tccagtttgt
     6421 agaaaagagt acaatgcttt attaaaagag tatcttgact caaaagctga tatattgtgt
     6481 gatacttgca atgagagaag agaaaaaaat ccaatgaggg ttatagactg taaaaatcct
     6541 acatgtaaag aaaatatcaa agatatacca tttattgcag accatttgtg tgatgattgt
     6601 aaatctcact ttgataaact tcaagaatac ttaaaggaaa tgaatataaa ctttgtaatt
     6661 gataaaacaa tagttagagg cttagattac tatagaaaaa ctgcttttga aattatatct
     6721 aatgatatag gagctcaaag tactgtatgt ggtggaggaa gatatgatgg tcttgttgaa
     6781 caattaggag gaccaaaagg tataagtgga attgggtttg gactaggaat tgaaagactt
     6841 ttacttacac ttgaaggtaa tggaattgaa atagaaaacc ctcaatctac ggatatattt
     6901 atagtaacta taggtgaaga agcaaataca agaagcttta aattgttaaa agacttgaga
     6961 caaaatcata taagtgcaga taaagaccat atagagagaa gtgttaaagc acaatttaaa
     7021 tattcagata aaataaattc aaaatttaca atagtaattg gagatgatga acttaaaaat
     7081 gacactgcga cattaaaaaa tatgaaaaca tcagagcaaa ctactgtaaa attaagtaca
     7141 ttagttgaag aattaaagca aaagctgtaa aataagaaag tatgatgttt tgtgtattag
     7201 ataaatatat agcattaaaa ttaaataaaa tggaggtagt atcttggaaa ctttaaaggg
     7261 tttaaagaga actcactact gtggagagtt gagagaaaaa aacataaatg aagaagtcgt
     7321 acttatggga tgggtacaga aaaaaagaaa tttaggtggt cttgtatttg ttgatttaag
     7381 agatacaagt ggattatgtc aaatagtttt tgatacagat gtagataaag aagcttttga
     7441 aaaagctgaa aaattagggg ctgaatttgt aatagctgta aaaggaaaag tatgcgaaag
     7501 acaatctaaa aaccctaata tgccaactgg tgatatagaa atatttgcaa ctgaacttag
     7561 acttttaaac aagtcagaaa ctccgcctat ttatataaaa gatgatgatg atgtttctga
     7621 agcactaaga ttaaaatata gatacttaga tttaagaaaa ccatctatgc aaagaaattt
     7681 aaaattaaga cataaagtaa tgaatattac aagaaattac ttatcaaaca atagattttg
     7741 cgaaatagaa acaccatttt taatagctcc aacaccagaa ggagcaagag attatctagt
     7801 accaagtaga gttaatccag gaaaatttta cgcattacca caatcaccac aattatataa
     7861 acaattattg atggtaagtg gtatggatag atatttccaa atagttaaat gctttagaga
     7921 tgaagactta agagcagata gacaaccaga gtttacacaa atagactgcg aaatgtcttt
     7981 tgttgagcaa gaagatgtta tgagtatgat tgaaggtttg cttgaagcga tatttaaaga
     8041 agttttagat gtagaattgg cacttccact tcctaagatg acttatgcag aagctatgtc
     8101 taaatatggt tcagataaac cagatactag atttgggtat gagttaacag atatatctga
     8161 tgttgtttgt aactgtggat ttaaagtgtt tgctgatgct acacaaccag gaaaaagtgt
     8221 tagaggtata aacgtaaaag gcaaagctga cgactttact agaaaacaaa tatcatcact
     8281 agaagaacat gctaagacat atagagcaaa aggtcttgct tggatgaaag taggacaaga
     8341 aggagtaact tctccaatag caaaattctt caatgaagaa gaaatgaacg ctatacttac
     8401 aagaatgaat gctgaagtag gagatttact tttatttgta gctgataaaa actcaatagt
     8461 atttgatgct ttaggacaag ttagacttga agtagcaaat aggcttaatc tattggataa
     8521 aaatgtatat aacttactat gggtaactga gttcccagtt tttgaagaag atgaagaaac
     8581 aggcacattc tcagcaatgc atcacccatt tacatcacct atggatgaag atttagacaa
     8641 gttagaagaa ggtgataagt catctttaag agcgaaagct tatgatattg tattgaatgg
     8701 ttatgaaata ggtggaggaa gtgttagaat atcaaattct gatgttcaat caagaatgtt
     8761 taaagcactt ggttttacag aggaaagagc taatgaaaag tttggttatt tattagaagc
     8821 atttaaatat ggtactcctc ctcatgctgg tctagctttt ggtcttgata gacttgttat
     8881 gttacttgca ggaactgata atataagaga agttattgca ttccctaaaa atcaaaatgc
     8941 tgtttgccct atgacaaatg ctcctacatt ggcggaagat gagcaattag aagaattaag
     9001 tataaaagtt gatataaaag ataatgaata atatgtagtt gcaagaaact aaaatgtaaa
     9061 aagtctggat at
//
LOCUS       DAMJZE010000156         9018 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJZE010000156 DAMJZE010000000
VERSION     DAMJZE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.160"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(93..827)
                     /locus_tag="PC541_003050"
     CDS             complement(93..827)
                     /locus_tag="PC541_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2840492.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            952..1668
                     /locus_tag="PC541_003051"
     CDS             952..1668
                     /locus_tag="PC541_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2840493.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1802..2494
                     /locus_tag="PC541_003052"
     CDS             1802..2494
                     /locus_tag="PC541_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840494.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2484..3665
                     /locus_tag="PC541_003053"
     CDS             2484..3665
                     /locus_tag="PC541_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2840495.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3947..4402
                     /locus_tag="PC541_003054"
     CDS             3947..4402
                     /locus_tag="PC541_003054"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2840496.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4392..5117
                     /locus_tag="PC541_003055"
     CDS             4392..5117
                     /locus_tag="PC541_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2840497.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5089..7470
                     /locus_tag="PC541_003056"
     CDS             5089..7470
                     /locus_tag="PC541_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2840498.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7675..8904)
                     /locus_tag="PC541_003057"
     CDS             complement(7675..8904)
                     /locus_tag="PC541_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2840499.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         3526 a         1046 c         1532 g         2914 t
ORIGIN      
        1 atataataaa taaaaaagta agattgtgta gattatataa agattattta caattaatac
       61 aatcttgctt ttttatttaa aaattttaat tattatttaa aattttttat cttctcttta
      121 tcccatataa acaatttctc agaattattt aaagcaccaa atacatttct tttaaaatca
      181 ggcatctctt tatttaaatt agcaataaga ttttttatgt actctctcct agtatgacca
      241 tttgcagggc agggatttgt gataatagga tagttagaat cttttgaaat tttcttagtc
      301 atatactctt ctatgtaaac cataggtctt ataactgtta aatcctgtct gtctaggtat
      361 gtctttgggg aaaaacaatt tactctaccc tcataaaaca tagacattaa aaatgtctca
      421 attgcatcat ctttatgatg accaagagct actttattac atccaagttt tttagcatta
      481 tcgttcaaag cacctcttct taaattagca cacaatgcac aaggattctt ttcttgtctt
      541 atatcaaata caatttcttt tatgtcagtt tgaaattcat aaaaaggaac atttatttct
      601 ttacacaatt tatctagtgg agaattatca acacctccag tatttaatgt tatggctatc
      661 aattcaaagt tttgtggaga aaatctttga taacttttaa gtatatgaag tagagttaaa
      721 ctatcttttc caccagaaag tccaaccgca attttatcat tttcttgtat catatcaaaa
      781 tcttgtatag ccttacgagc tttacttaga agtttttgta tagacatttt atcaccatcc
      841 tttaaaaata ttaaatttaa gatttattta agatttactt ataaaatact taagtcttat
      901 actctaaaat taacattata gcaaacataa caaaaaggag aagatttaat tatgaaaaaa
      961 tttaaattaa taggagccat tattttagta gtatttataa gtgtatttat atctaaagat
     1021 agattactta agaaagataa agatccaata gtagctgctc aacatgattc agaacaagat
     1081 gttgaggcaa attctcaaaa taaaaatcac tcaaatagta tatcaaatat attattggta
     1141 aacaagacaa atggaattag caaaaactat actcctgaaa atattactaa ggtaaacata
     1201 ccttttgttg aagaagcaac agaggaagaa aaacaaatgg caggagaacc tgcaaaggca
     1261 gttgaagatt tagttaagca agctaattca gaaggaatac agtttttagg gtcttcagca
     1321 tacagatctt atgatactca actggatact tatacaagaa gggtaaaatc tcaggggcga
     1381 gaaaaggcag atgcgtatgt agcaaagcct ggttacagtg agcaccaaac tggactttgt
     1441 attgatttaa caaatccaga aagatggttt gttggaagta caaaagaagc taaatggttg
     1501 gcagaaaatg ctcacaagtt tggatttata ataagatatc ctgaaggtaa agaagatata
     1561 acaggtacag catatgaacc atggcatatt agatatgttg gtaaggatgc tgccgaggaa
     1621 atttattcaa aaggattaac tttagaagaa tatttacaaa ataaataata taaatgtgta
     1681 aataatttgt attatattgg aaatttagaa attaataaat ttatagatta cactaactaa
     1741 aatatagtat aattttatat gaataaagct atatacaaag attaagttag gatgtgtaaa
     1801 aatgaataat acagttcttg ttgtggatga tgaaaaggaa ataagagacc ttatagagat
     1861 atatctttta aatagtggat ataatgtaat taaagctgaa gatggaaaag aggctctaga
     1921 tattttaaaa aaggaaaata ttcatttaat gatattggat ataatgatgc ctaaaatgga
     1981 tgggatagaa gtttgtagaa aagttagaga aagtttgaat ataccaatac ttatgttaag
     2041 tgcaaaatca gaagacatgg ataaaataca agggataatg actggtgcag atgattattt
     2101 gacaaaacca tttaacccac ttgaacttac tgtgagagtg aagtcgctga ttagaagagc
     2161 atattatttt agtggtgcta ataatgaaga taaagatgtc ataaaggttg gagtagtaat
     2221 tatagataag gaaaaacata gtgtaacagt ggctggaaat gaaataattt tgacatcaag
     2281 agaatttgat atattatatt tactagcaaa taataaaggc agagtatata gtacagaaga
     2341 aatatttgaa aaggtatgga aagagaaata ttaccaatct aataatactg taatggttca
     2401 tatgagcaga ataagggata aaattgaaaa atatacagat ggagaaaaaa taattcatac
     2461 tgtatgggga gtaggatata aaattgaaaa ataaaagttt attaaatcat atgtttcgac
     2521 ccatattaga tcttataata agctgtgctt taacttcaat gcttatttta acaagtatgt
     2581 atataataaa atcattatat gtcaatagtg atatgtctgt agttaatcaa cttgtatatc
     2641 aagcaagaga tttcagaagt cttatagggt atgatttatt ttatccattt ttatttacaa
     2701 tcacatcatt agcaatttat tccatgatat cgtacaagag aaataagcaa attgtagatt
     2761 ttataagaaa gactaataga aaaaatcaaa aattagaaat cattaaaagt gctgtaaatt
     2821 taatggatga aggtaactta gaaaaaagta tagatattga gtatgattta gatatattaa
     2881 aggataaaga aaaggatgat attgatgaac tagcacataa aatcaataat atagtccatc
     2941 aacttagaaa tataactatt gaagagagac aagctcagca gacaaaaaca gatttaataa
     3001 caaatgtgtc acatgactta agaacacctt tgacatccat aatgggttat ttaggagtga
     3061 tagaagaagg taagtataaa gatgaagttc agatgatgta ttatgttgat attgcatatg
     3121 aaaaatcgaa aaatttaaat gttcttataa atgatttgtt tgaattgact aaaatgcaaa
     3181 ataatacaat aaaattaaac aaaatagaaa taaatttagt agagttatta agtcaagtag
     3241 tatctcaatt tgaaatttat tttaaacaag aatttatggt tagtagaatt aattttaatg
     3301 aagaaaaatt aattgtaaaa gctgatccaa ataaattggt aagagcattt gaaaatctta
     3361 taactaatgc tgtgaaatat ggaaaagatg ggcattatgt agatatagtc acagagaaaa
     3421 aggaagatat ggcggttgta aaagtgataa attatgggga accaattcca gtgttagatt
     3481 taccaaatat atttgataga ttttatagag ttgaaaaatc gagaaataga aatgttggag
     3541 gttctggatt gggtcttgcg attacaaaaa atattgtaaa tttacatgat ggagaaatta
     3601 ctgtttcaag cgataaatat caaactgtgt ttgaagtaca actgccaata atacaatcag
     3661 cttagttata aaataaaagt acgattatgt gatataaata ttataacaat aaagtgttta
     3721 caaaaataaa aaagtaatat ataatttaag tcagtttaat aagatactta tcaagagtta
     3781 ggggagagag ttagctcagt gatcctatac caacctttta tcaggtggtc ctgctaacaa
     3841 cgatgagatg acttgaaata atatactctt cttatcaata atagatagga agagtttttt
     3901 ttagaattaa tgtaacaaat tttaaagtat aatggaggat taaataatgg aaaaagtaga
     3961 aagttttaag ctagaccata caaaagtaaa agctcctttt gtaagaaagt gtagtgtact
     4021 tgatggagta aagggagata aagtaactaa atttgacttg agatttttac agccaaatgt
     4081 agaaagtttt ggaactgctg ctatgcacgg tttagagcat cttttagcta cttatttaag
     4141 agatacatta gatggtgtaa tagacttatc tccaatggga tgtagaacag gattttattt
     4201 aatactatgg ggagatgtag atgccaaaac agttaagatt ggtttagaag aagcattaaa
     4261 aaaagttctt gaatcagata aaatgccagc agctacagct atagaatgtg gaaactacag
     4321 agacttatct ttatttgggg caaaggaata tgcaaaagat gttttagata aaggattttc
     4381 tttaaatata tatggagaat aatttaattg gaagagaaga tataagcata aatcaaagtg
     4441 aagttttaag atatttacaa tatagaggtc aagagataga taaagacctt aaatatacta
     4501 ttgatgaatg tgtagctata actaaaaata aaataaatcc tagatattta tctaggattt
     4561 atcctattaa aataaataaa gataatgttg tagaattaga gggaacaaat ttaacattag
     4621 agagtaaaga tgtatatgaa cttttaaaag aatgtaatga gtgtgtaatt atggcagcta
     4681 ctatagggat agatatagaa aaagaaataa gacgacactc ttattcaaat ttaacaaaag
     4741 gactaataat tgattcatgt ggaacaacag caatagaaga agtgtgtgac attgttcaaa
     4801 ataatttaga aaaggttttt ttaaagcaag ataaatttat aactatgcga tatagcccag
     4861 gatatggaga tttaccaata gaaaaaaata tagatatatt aaacgttttg aatgctcaaa
     4921 aacaaatagg tctaacaatt acttctaatg gaattatgat accgagaaag tcagtagtgg
     4981 ctattatagg tatttctaat acaagaataa ataaatctaa gaaagaaaag tcgtgtgaaa
     5041 attgtaaaaa ctatagaaac tgtacatata gaaaaggagc gaactcttgt ggaaataaga
     5101 caatatctga aaaataatat attaatattt gatggtgcaa tgggaacaat gcttcaacaa
     5161 aaagggttaa agttaggtga aaatccagaa gtatttggtc ttcaaaatcc agataaactt
     5221 atagaaatac atacagcata cttagaggca ggttcgaatg taattcttac aaatactttt
     5281 ggatgtaatg aactaaaatt agatagtaag tatactgtag aagaagtgat tgacaatgct
     5341 gttttggtgg ctagaaaagc tatagaaaat gtagataata ctaaaccaag atatgtagct
     5401 ttagatattg gtcctatagg agaaatgcta gagcctatgg gtactttaag ttttgataaa
     5461 gcatatgaaa tatttaaaag acaagtacta caaggcgtta aatctggagt tgatgtaata
     5521 gtaatagaga ctatgatgga tttatatgaa gctaaagtag ctgtattagc agcaaaagaa
     5581 aactctgatt tacctatttt ctgtactatg acatttgatg aaggtggaag aagttttact
     5641 ggatgtatgc cagaatgtat ggtggctact atagaaggtc taggtgtaga tgcaatagga
     5701 gttaattgtt cattaggacc taaacaactt ttaccaatag ttgaaaaaat agcatcaaga
     5761 gctacagtcc ctgttatggt tcaagcaaat gcaggtcttc caaatatagt tgatggagag
     5821 gctatatatg atgttgatgc aaaagaattt tttgaagggg ttaaaaagtt cgttgaagtt
     5881 ggtgctacta taataggtgg atgttgtggt acgaatccaa gttttataaa agaaatatct
     5941 gagaatataa attctgttac aaagggatgt atcgagaaaa ttgataaatg tgtagtctgt
     6001 tcaccatcaa aatttgtgga agtacaatcg cctacagttg ttggagaaag attgaatccc
     6061 acaggtagaa aatctctaca agaagcatta aaaaatgaaa atgttgatta tgctataaat
     6121 ttaggtttag aacaagtaaa tgctggagca caaatacttg gagtaaatgt tggtcttcca
     6181 gagattgatg aaaaaaaact aatgccaaaa ttaataagag agattcaagc agtagtagat
     6241 acacctttac aagttgattc ttccaatgta gaggccttag agcaaggact tagatattat
     6301 aatggtagaa caatagtaaa ctctgtaaat ggcaaagagg agtctttaga aagtatatta
     6361 ccaatagtta aaaagtatgg ttcatgtgtg gttggactta cacttgatga aaaggggata
     6421 cctaaaaagg ctgaagaaag atttgaaata gcaaaaagga tagttaatag agcagtttct
     6481 tatgggataa aaccaaaaga tatatttata gactgtttat cattaactgt ttcagcacaa
     6541 caagaagaag caatagaaac tataaaagct ataacaatgg taaagacact aggagtaaaa
     6601 actatattag gtgtttcaaa tatttcattt ggtcttccaa ataggaaagc acttaatgct
     6661 tcttttctaa cattagcttt aggggcaggg cttgatttag caattataaa tccaaatgaa
     6721 tatagtatga tggaagctat caattctttt aaaatattaa ataacacaga taaaggatgt
     6781 ataaactata taaatcaata tagcaatata aataattcaa aaaaatcgga ttcatctaca
     6841 aagattgata aagatttgcc cttggatatt ttagttgaaa gaggattaaa agatgaagca
     6901 aaaaatgtaa cactgaatct tttaaaagag aaggatgaaa attatatatt agatgagatt
     6961 ttaattcctg ctcttgataa agtaggaaaa agatatgata gtggagatat atttttacca
     7021 caattgattc aatcagcaga aacggtaaaa gtttcattaa atacaataaa ggaaacttta
     7081 ttaagtaaaa gtagcaataa tgtatccaaa ggaaaaataa tagttgctac agtaaaaggt
     7141 gatatccatg atattgggaa aaatatagta aaaataatgc tagaaaatta tggatatgaa
     7201 gttatagatt taggtaaaga tgtccctatt gaagaagtag tggaagttgc taaaaagaga
     7261 aatataaaat tagtaggatt aagtgcactt atgactacga ctgttcaaag tatgaaagat
     7321 acaattcagg cactgagaga taatgagata aatgcaaagg tatttgtagg aggtgcagtt
     7381 ttgacagaag aatacgctga agaaatgggt gctgattatt attcaaagga tgcaaagtct
     7441 gctgttgaaa tagcaaagtt aaatttttag catataaatt aaaataaagg ttttactaaa
     7501 taggatattt tatatataat ataaatataa gttttagata cttatgttta aaacaatagg
     7561 aattaatcta taataatgag attaattaat ataaaaaata tttatcatca acattaaaaa
     7621 gggctgggaa gtctggtata acttccatca gcccttttta gttaaaatca tatgttaaaa
     7681 agcccaatta ccatttttaa aaatctgtat ttgctttcca tcataagtct caccaataat
     7741 atccatatca gcagaaccaa tcataaaatc tacatgtgtt aaactgtcat ttacaccatg
     7801 ttcatctaat tcttcatctt ttaaattgtt tccaccttcc agacaagtct tataagcaga
     7861 accaattgca aagtggcaag aagcattttc atcatataga gtattataaa aaattatatc
     7921 cgtattggat attggtgagt tgtatggaac aagagcaact tctcctaaat aatgagagcc
     7981 ttcatccgtt tcaataagtt tttctagtgt atctagtccc ttttcagcag agaaatctat
     8041 tattttacca tctttaaatg ttaagaagaa attgtcaatt atgtttccac cataaacaag
     8101 aggttttgta ctataaacag ttccatttac tttaaactta tgtggcattc caaaaatctc
     8161 ttcagttgga atattcggat taaaacttat tccattaggg tctttggatg ctccacttag
     8221 ccaaacatga ccctttggca agtctagagt caaatcagtt tttgaggatt tatattttaa
     8281 agttttaaag ttatttttat ttagaaagtc cattttagat ttaagatttt cattatgttc
     8341 ttcccatgct tttatagggt cctgaccatc tactcttgaa catttaaata tagcatccca
     8401 cagtttttct attgctttat cttctgataa atttggaaac actttttttg cccaatcttt
     8461 tgttggcaca gagactatac tccatttaca tttatcagtt agagtgtaag aacgccattc
     8521 ttttaaagct tttccagatg ccttctgaaa atttgcaact ctttcagggt caacatcttt
     8581 taataaatct ggattttggg catagactga taaaaatgcc ccaccttctt ttgctatatc
     8641 tacatattgt tctgctgtcc atttggggaa ctcatgaaat gcttcatctg gagcatataa
     8701 gtatttaata agagtacttt cttcatcaga ccactctata taaacatttt tagcaccaca
     8761 tttatatgct tctttaagtg catttcttgc aaaatcagca cattctattg gtgactttat
     8821 aagtagaatt tgacctggct gtatgttaac tccaactttt attgctaatt cagcgtattt
     8881 ttctaaattt ctatttaaat tcatatttat cctcctatat ttttaatatt gaataactta
     8941 taaagtatta taacattact tatgttttta atattgaaca acttataaaa tattataact
     9001 ttacttagat tttgggtc
//
LOCUS       DAMJZE010000157         8967 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJZE010000157 DAMJZE010000000
VERSION     DAMJZE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.161"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..2001)
                     /gene="recJ"
                     /locus_tag="PC541_003058"
     CDS             complement(<1..2001)
                     /gene="recJ"
                     /locus_tag="PC541_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2840500.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDK"
     gene            complement(2014..3678)
                     /locus_tag="PC541_003059"
     CDS             complement(2014..3678)
                     /locus_tag="PC541_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2840501.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3976..4473)
                     /locus_tag="PC541_003060"
     CDS             complement(3976..4473)
                     /locus_tag="PC541_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2840502.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4502..5881)
                     /gene="scfB"
                     /locus_tag="PC541_003061"
     CDS             complement(4502..5881)
                     /gene="scfB"
                     /locus_tag="PC541_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2840503.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6177..6323)
                     /locus_tag="PC541_003062"
     CDS             complement(6177..6323)
                     /locus_tag="PC541_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2840504.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6353..6931)
                     /locus_tag="PC541_003063"
     CDS             complement(6353..6931)
                     /locus_tag="PC541_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2840505.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(7299..8729)
                     /locus_tag="PC541_003064"
     CDS             complement(7299..8729)
                     /locus_tag="PC541_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2840506.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         2841 a         1619 c          833 g         3674 t
ORIGIN      
        1 tttatcaata ttaggagaaa atataagctc taatttatca gtatttttgc taacatcttc
       61 aataatattg taatttatgt taaaatccaa atctataagt gatatatctg acattgctct
      121 ataaaatcca tttatagaat tagatattat tagtgtatct ttttcaaaat aatcaaatat
      181 atttatatct gtatttccat caaataattc aaaatcagac acagatatat tatacaggct
      241 ttgagtatct tttggtatta tttttgacat taatttcaat gaaagtttgt cattacttgc
      301 acttttaggt cgagccaatc gtatatcttt aagtaaaaat tgtactttcc tatttcccat
      361 atagttattt tcatctaatt ggaatagtac atctaccttg tctcctactt tatacattga
      421 ttttaaatga ctcatattaa atcctataca ttcatagctt ttctcttttt ctatgcttaa
      481 tttcagatgt tgttgattct tacctataac tttcaaatct tttaaaatat aatttctaac
      541 tataaatcta ggattaggat tatttaatcc aaagggttct aacttatgta attcttctac
      601 aagatttaaa tttattacat tttcttgtaa ttcaaactca acatttacat tctctatcat
      661 atcatcttca gttaaattat aatctgcaaa tttatttatt tcatttgcta aaatttctac
      721 attgtctgaa tccaaggata accctgctgc ttgttcatga ccaccaaact tagtcatcaa
      781 gtctttgcac tttatgagag cttcaaatat attaaatcct tttattgacc tcgctgaacc
      841 agtagcctcc ccattttcta tacctagaag aattgttggt ttgtagtatt tttcagttaa
      901 ctttgatgct acaattccta ttattccatg ttgccaaccc tctttagcta aaaccaacac
      961 cttgtcatca ttgtatctgc tattagactt tagcatttcc tcagcttcat gatacatctt
     1021 agcttctatc atctgtcttt cattattttt ttcttccaat atactagcta tttctactgc
     1081 ctcttcttgt gattgagtag taaatagctc tactcctaag taagaatatc ccaatcgacc
     1141 tgaagcattt atcctaggac ctattgcaaa acctatatga gaagaaccta tcttatcact
     1201 ttctacacca caaactttta taagctccct taatccaata tttttgcctt ctttcattga
     1261 ttttaaacca ttttttacaa ttattctatt ttcatctata agtggaacaa tatcacaaat
     1321 agttgctagt gttactattt ccaaataatt atacatacta gttttaaatt cttcttttgg
     1381 agtcaaggct tgaatcattt taaatgctac accacatcca catagtgaat caaatggata
     1441 attacaatct tcttgttttg gatttattac tgcaaaggct gatggaattt cactttgaca
     1501 ttcatgatgg tctgtaatta taacatctat gcccaaatca ttcgctgtat tgacctcaga
     1561 cactgatgta attccacagt caacacttat aatcaagtca catcccctag agcttataag
     1621 cttaatagca tcttcattta taccataccc ttcttctaat ctattaggaa tatagtaatc
     1681 aacattgtaa cctatactct taaaatataa gcacaagata gatgtagatg atacaccatc
     1741 aacatcataa tctccgtaaa tccaaatttt ttcgttcttc tcaactgcta actttattct
     1801 atcaacagct ttttgcatat ctttcattaa aaaagggtct cttaaataat ctaaagatgg
     1861 attcataaat atctctgaat ctttttcagt acatatccct ctatttttta aaatttggca
     1921 gatttccggg gaaatattaa gttttttgct aaattcactt tcctctatct tacctttata
     1981 cttcaatatc cattttttca aatttaattc cccctaatct tttttgtatt ttgacctaat
     2041 acttcttata actaagcaaa ggcctattat aaaggatata aagaaacccg ctattgcagt
     2101 aaattttatc catatattat tttctatatt tggagatgca attataagag aagaacctac
     2161 tataatagat gccaacacta aactcaaaga tatttgagat gttagctcta ttatacaatt
     2221 ttctaacctc gtcattttaa catcttcaat ttgcattttt atattatttc tttctatatt
     2281 ttttaaaatt gcttttaatt gctttggaat agtttttatg tccagtaata tttcttctac
     2341 attttgtctt gaacttaaaa caacattttt agggttaaat ttactcttat aatgatgttt
     2401 cataaactct tttcccatag aaccaaaaga aaaatctgtg tttaaagttc tacttgttcc
     2461 ttctaatgtt ataactgtct ttgcaagagt tacaaattgt gcaggcatga ctattttata
     2521 ctgtctaaaa aacctaaata gttcatttaa tatatctgtt acatttattt tttctataga
     2581 aatatcatag taataatgta ttagataaag taaatcctgt ctaagtccct taatatcagc
     2641 ctctccattt aatgcatcca tttctatcaa tagatatatt atcttgtcaa cattcttttc
     2701 tacacctgct aatgcaattt catttaattg gtttaaagtt ttattatcaa taattccaat
     2761 cattccaaaa tctatatatg ctatcttatt tttagcaact acgaatatat tacctggatg
     2821 tgggtctgca tgaaaaaatc catgagataa tacctgtgta aaaaatgacc taacgcctat
     2881 ttcaacaata gtttttgtgt tataacctag tctccttatt ttttctacat cacttaactt
     2941 tgtaccatta actttctcca ttactaaaat tttcttagta ttatattctg aatatacttt
     3001 aggtatatat acttcatcac tatttttaaa tatcctagag aatttagttg catttatagc
     3061 ttcaaatgta taatcaagtt ccctcattaa ttgagtttga aattcttcaa tcatctgtac
     3121 taaatctaaa ttaaaatcct tttttaaatc ttttaaagta tttgcaatag ttctaagaat
     3181 ctctaaatca gattttatta ttttctctat atttggtctt tgaattttta cgattacttc
     3241 ttctcccgtt ttaagtacac cttcataaac ttgaccaatt gaagctgctg ctattgcttc
     3301 ttctttaaat tctaagaata tttcttctat acttaaaccc acttcttctt taaatatatc
     3361 aatagctata tttgaatcaa atttctctac atcatctctg agttttgata tttcatcaat
     3421 tatatcctgg tctaacaagt cttttcttgt actcaatatc tgtcctattt ttatatatgt
     3481 aggtcctagt tcttctaaaa ctcttttcat tctctcacca gttgtcatat tttttatttc
     3541 ttctggtggg tcaaatagtg gtattttata ggcaattcct tctatattta gtctttctac
     3601 aataaaacta aaaccatact ttattaaaac atatccaatt tctctatatc gctttaaatt
     3661 tctataactt attctcaaat cttttcactc ctaagatagg tatacccaaa aacttacacc
     3721 ttaatttatc gtatatgaca ttatttcacc aattgatatt ctaatatcct atatcctctt
     3781 taatatttaa ttttatcaca tatactacca attttattaa aattatttaa ctcagttaat
     3841 ttttattttt gacattttat atctggttta atcaaatact ttttgaaaac taaaatatca
     3901 acaaaatcta ataaaataaa ttagtggttg cctcaaaaca gatttaaata tgttttgaaa
     3961 caaccactct ttaaactaat atttttgatt ttgtgctgcc tctaaatacc attcataaga
     4021 ttcatcaata tattttttat cttcttctgt aagctctcta acaaccttac caggagaacc
     4081 cattatcaaa acacctggcg gaaacttctt attagatgta attagtgtac ctgctcctat
     4141 cagagtatat tctcctattt cagcattatc caatattatt gaacccatac caattaacac
     4201 attatcacta attttacaac catgaactat agacctatgc ccaactgtta cattgttacc
     4261 aattatagtt tctgtatctc catgcactat agaacaatct tgtatattag tgttttctcc
     4321 aatagttatt ggtccttcat cacccctaac tactgcattg taccatatac ttgaatcttt
     4381 gcctatttta acatttccaa ttacatctgc tgattttgcc acaaatacac tttcgtcaat
     4441 taaaggttta tcttctaaat aatctctaat catatataaa caactccttt aaaatataaa
     4501 tttagttggt tgctccttct aacattaact tagcttgtat cattatagag cactctgtac
     4561 gttttctttg catttcacat cctaattcat atggttttga tatgtcatta ttaaagttta
     4621 tcgcatttgc atgacatcca cctgaacagt aaaacttatt ccaacattgt ttacactctt
     4681 ctttagtata tacatgagct tctctaaaca tattttttaa attttctgga agtactattt
     4741 cttcatcaaa tatatttgcc attttaaatt cttcatttcc aacaaattga tgacatggat
     4801 agatatctcc atttggagtt acagaaagat actcatttcc agctccacat ccagttattc
     4861 tctttattac acatggacct tgatttaggt ctatcataaa atggaaaaat ttaaatccat
     4921 ctccttgtaa ttgtctatct gcatactcta cagcaaattt ttcgtactct tcaaatatct
     4981 taggtaaatc ttcttctcta agtgcatatg gattactctc atctccaacg actggctcta
     5041 cagaggttaa tttaaatcct aaatctgcaa aatgcataac atcttttgaa aaatctaaat
     5101 tatctcttgt aaaagtacct cttatataat aatacttatc ttttgctctt ttttccacaa
     5161 gtttcttaaa tcgtggtaat gtaatatcat aacttccttt atcatttaaa gttggtctca
     5221 tattatcatt aacttctttt cttccatcta aacttaaaac tacattatgc atattttcat
     5281 ttatataatc tataatctca tcatttagca gtacaccatt tgtagttatt gtaaatctta
     5341 tatttttatt ataatctttt tctacacttc ttccatagtc aaccagttgt tttacaactt
     5401 caaagttcat caaaggctct ccaccaaaga aatctatctc taggttcttt ctacttcctg
     5461 aattagcaat caaatagtca attgctgcct taccaacttc aaaactcata agttcttttt
     5521 ctcctccaaa atcaccttga gctgcaaaac agtatttaca ttttaagtta caatcatgag
     5581 ctacatttaa acataaagct tttactacag gctctctatg tacaaagctt ggatgatatt
     5641 gatatgtatc ttctgtatat aaaagaccct ctttttccaa atttaatatt tcttcatatg
     5701 cttcatttat tttttcttct tgatattttg attttaatat ctctaatatt tcttctttac
     5761 ttttttcttt ataaaaatct aacaaatcgt atgcaacatc atctaacaca tgaacagctc
     5821 caccattcac atctaaaacg atattatatc cattcataga aaatttatgt atcatactca
     5881 taccctacct cctaaattac tatctatact aaagtttttt attattttaa ttaaacttta
     5941 cccttcatta tatcataagt ttacataaaa tacatacaat acaaaaatga cttactctcc
     6001 gtaagtcatt tcattaaata cactatatcc ccctaaattg gcgcactaat atattaccaa
     6061 ataggtatta ggctcctata aaataaattt tgtatctgat tttatattgt tttaatctta
     6121 ttaaaatata ataatttctt ttttcctgat aattttgtat aaataagtgc ttgtatttaa
     6181 tttgtcttct ttagattgct gatttctttg ggtactttag gctgatgaat aatccaagga
     6241 caggtactat tagctgacaa aatagcagtg ctaagtgcca atgagctagc aaacttcata
     6301 aatcttacaa taaacttttt cataacttaa ctctccctca tacatatttt acttatactt
     6361 tagcttttaa cttacctaat actaacatca caaaaatcca aaacatggca aatgcgctat
     6421 atgtgaaata atctacaaat atactcaaag aaagacttga aattgttatc aataacacta
     6481 ctgtagatat aaatatagca gttttcttat aaaccaactt ttccctatca cttatagggt
     6541 tactcctatg ttctattggg cacagaacac aaattccaac ccaactaaca cttgcaataa
     6601 tcataataat atttttaaat aagtctatat ttatattttc tataattaag attgtggata
     6661 aaattataaa aataaaagtt aataagcatc taaaataatt gtctgcatga taccctcctg
     6721 aaaattgtct tattggacaa taacacatta aaaaaaataa tacatatgta aatttaccaa
     6781 ataaaaaacc tataaacagc atggtggtta tattaactat gaaagctatt aatatttcaa
     6841 atccataaga atagacttta gattcattat tatctatcat attattacaa attaaaacag
     6901 atgtcatttt ttcagcatat cttttaaaca tttttacctc caaaaaaatt ttacatatat
     6961 agagtacatt cttttttgag aaaattcaat atactttaca aaactagttg ttaaaaatgt
     7021 aaaactggtc aaaaaaagac ctatataatt ttcttcttat tatttatata acaaaacaat
     7081 tataataaaa caattttatc aagctttata tgcataaaaa gcctatgtca aaatagaaac
     7141 aaaccttttc ccctttgtta aaaggctaaa aatatatttc atttcatata aaaagagtac
     7201 ctcacaaact aaaaagttcc ttttgagata cccattttat ttattacaaa atatcaaatt
     7261 ttatttattt gcatatattc cccatatatt ttagcctttt aactttgttt ttgttttaca
     7321 tactgtagtg cttttctatt ctgttttact gcttcaagac aaactttttc tgtttgatta
     7381 tcaacaaatt ctaaagctct ataattttgc tttactgctt ctaaacatat tttttctgtt
     7441 tgattattta catatttcaa cattctataa tcttgtttta ctgcttttag acaaacttct
     7501 tctgtttgtt ctttcacata tttaagcgct tcataacttt gattaactgc ttctaaacaa
     7561 atcttttctg tttgttcctt tacataagct aaagcattat agttttctct tactgcctct
     7621 aaacatatta attctgtctg attttctaca tatttaagaa tttttccatt ttgctttact
     7681 gcttctacaa atattctttc tgttggtatc tttacataac ttaatatttc ttttatttca
     7741 agttcttcaa atgaacctct tactgcttct atacatatat tttcagtctg tttttttaca
     7801 tattgcagta cttttccatt ttgtctaact gactccaagc aaatttcttc tgtttgctct
     7861 tttacgtact gtaatgctct tccatcaatt cttactgctt ccaaacaaat ctcttctgtt
     7921 tgcttattta catactgtag agccatataa cttcttttta ctgcttccat gcatattttt
     7981 tctgtctgtt catttacata atacaatgca aagtcattct gttttactgc ttctatacat
     8041 atttcttcag tttgctcttt tacacaagat agtgcactgt agttttcttt tacagcttct
     8101 aaacatatac tattactttg ttcctttaca taagctaaag attttccatc tttttttaca
     8161 gcttctaaac atatggctgg agtttgctct tttattcctt ttaatatctc tgcaacatca
     8221 tttttagatg ccctttctat aaatgctaga catacatctt ctgtttgctc ttccacaaat
     8281 ttaaatactt ccatgacatc ataagttgat gcatttttta tagcctcaat tagtacattt
     8341 tctgtcttat tatttatgta gtacaaagct ctgtagttct gttttactgc ttctatacaa
     8401 atatcctcat tttgatttgt tatatactta agagatttat atgtctgttt tactgcttcc
     8461 atacatactt tataagtctt gtcctttaca aactcaatta tagtaccatc ctgctttact
     8521 gctagcagac acatctcttc tgtttggttt tctatgtctt ttaactctct gtaatcatat
     8581 tttactctgc ttaaatcatt taaatgtact ttgttatctt cctttaaatc ttcttcatta
     8641 ttttctatat catactcaaa ttcttcatca tctaaatagt catttttttc tggtaaaaat
     8701 atagatttaa ttttattctt tatatccata aaattatcct cctttaatat gattctatca
     8761 ttaaagtaaa tcgtttttaa gcacttattt aattaatttt atattaagtt tatttaaaag
     8821 tctattcatt gtcgatagat tatattttat attaataatt ttatcttata tatgtggggt
     8881 aataaagtta atagtggaca ttttccatcc cattagtata cactatttat atttattact
     8941 catttatagt gatttttttc aaatttt
//
LOCUS       DAMJZE010000158         8850 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJZE010000158 DAMJZE010000000
VERSION     DAMJZE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.162"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..480
                     /locus_tag="PC541_003065"
     CDS             <1..480
                     /locus_tag="PC541_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2840507.1"
                     /translation="AKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLST
                     KLIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKF
                     FASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            912..1166
                     /locus_tag="PC541_003066"
     CDS             912..1166
                     /locus_tag="PC541_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840508.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1202..1525
                     /locus_tag="PC541_003067"
     CDS             1202..1525
                     /locus_tag="PC541_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840509.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1799..1951
                     /locus_tag="PC541_003068"
     CDS             1799..1951
                     /locus_tag="PC541_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840510.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2145..3068
                     /locus_tag="PC541_003069"
     CDS             2145..3068
                     /locus_tag="PC541_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2840511.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3335..4150
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3335..3363
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4341..4493
                     /locus_tag="PC541_003070"
     CDS             4341..4493
                     /locus_tag="PC541_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840512.1"
                     /translation="MKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4613..5426
                     /locus_tag="PC541_003071"
                     /pseudo
     CDS             4613..5426
                     /locus_tag="PC541_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5478..5639
                     /locus_tag="PC541_003072"
     CDS             5478..5639
                     /locus_tag="PC541_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840513.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5967..6431)
                     /locus_tag="PC541_003073"
     CDS             complement(5967..6431)
                     /locus_tag="PC541_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840514.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6567..6884)
                     /locus_tag="PC541_003074"
     CDS             complement(6567..6884)
                     /locus_tag="PC541_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840515.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7167..7463)
                     /locus_tag="PC541_003075"
     CDS             complement(7167..7463)
                     /locus_tag="PC541_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840516.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7482..7895)
                     /locus_tag="PC541_003076"
     CDS             complement(7482..7895)
                     /locus_tag="PC541_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840517.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7879..8112)
                     /locus_tag="PC541_003077"
     CDS             complement(7879..8112)
                     /locus_tag="PC541_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840518.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8331..8705
                     /locus_tag="PC541_003078"
     CDS             8331..8705
                     /locus_tag="PC541_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2840519.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
BASE COUNT         3564 a          927 c         1413 g         2946 t
ORIGIN      
        1 gctaaattac aaagagaatt atcaagaaaa acaatcggta gtttaaatag aaataaagca
       61 agattaaaag ttgcaagact tcaagaacat atagctaatc aaagaaatga ttttctacaa
      121 aaactatcta ctaaattaat aaaagagaac gatattattt gcatagaaga tttacaagta
      181 aagaatatga ttaggaatcg taaactatct cgctcaattt ctgatgtgtc atggtcagag
      241 tttattcgtc aattagaata caaggctaat tggcatggta gacaaattgt aaaggtgggt
      301 aaattctttg caagttcaca aatatgcaat aaatgtgggt ataaaaacga ggaagtgaag
      361 gacttaaata taagagaatg gatttatcct agttgtaatg aaactcacga tagagatata
      421 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaattca aaataaataa
      481 cgtatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatatgt
      541 tagtatgcaa aattctgtga agcaggaaac ctgtgacttt agtcatggga ggttcaggct
      601 agtattactc attattgtat tcaaaagcta aagtggaaac ctagtgaata tatgaattta
      661 gaagttaatg agagagcatt agcagccgac tcaatactta ttaaaattga agatgaagaa
      721 gaaacaatga aagaagctga aagagagaaa aagagaggta gaagaaggta gaatttaaag
      781 ttaaaatagt tacattgcta atatatgaaa atgttacaaa tttggaaata gatggatata
      841 tatgttgtat agtataatgt agataatata tcaccaagta aaaaacaaca aataaatcta
      901 aattatgagg ggtggaaaat aaagatatta aacttacaat atgtatattt gcttttgtaa
      961 ttggaataat agtgttattt tgctgtatag gtctaggtca atttaatatg gattgtatta
     1021 taaaacaaaa tggtgggtca atagctaccg aacaatacaa catatattta gaacaatcta
     1081 tttcacaata tagaaatttt ggttctatac tggttttact aggaggaata ggaatactgt
     1141 ttgataaaag tgtcagaaaa tactaaaaaa gtatattttt atttaggatg tgatgaatga
     1201 tatggcaaga gatgtattta gtgtaaagat gacaccacta gaagcacaga aaatggtaga
     1261 aaaaaatttg gaagcagatt tagtttctgt tgatatttat aacctagaaa ataataagtc
     1321 tataatagtt acaatctttg aaaaatatta tgcaagaagc aatagtgatg caggtttggt
     1381 agtgatttgt gaaaatacta caggaaaaac acttgttaaa attacctcaa ctggtagtgc
     1441 aattggacta tttcagattg attggggaac aggtaataat ttgattaaaa gagtaaaaaa
     1501 aatacttatt gattatatta tttaattatt ttatgtagat attcttaggt tggtatggaa
     1561 tttgtactaa cctattaagt aatcaataaa actaaataaa catagaaagc acttacaaat
     1621 atgtaggtgc tttttattga aaaaatagaa aataaaataa aaagtaagat ataggaaaaa
     1681 tatgtaagaa ttatatgtta taataattgt agcaagaaga tgtaatctac aatttataga
     1741 gtggagttca tgtattaaaa aattatcctc ccaacgataa gaagggaggt gaatatacat
     1801 ggataatttt ttatttaatg tgttagctag tgtaacagct agttacatag tttatttgat
     1861 tagcaagtta atcaaaaaag taaaaagcca ctctaccgca aagagtgact taaagattga
     1921 tataaatttt aaatatcatc gtaaaaaata actttcagtt atagaacttc actctaccgc
     1981 aaatagattg tagttcttct tgtttttatt ataccacaaa ttagaaaaaa tattgcatat
     2041 aatatttttt atagtcaata aaaatatgaa attttttata cacaatgatg atgaatctaa
     2101 ctagatgaat aaatttaatt gtaaataaga aagtacttac ttaaatggta ggtgcttttg
     2161 ttttgccaaa ttatgctata atattgagtg agatgagtaa tttgtggggg aattttataa
     2221 tgtggatatt agtaccaata ataacaatag tattacttat aatagctgtt tcttctatgc
     2281 aatatatttt agttatgata gcttttttgt tgattatata ttcttttata gaaaagaaaa
     2341 tagttatggg tcttgtgtca gttctttttt tcacttattc aatttatctt tgtgcaactt
     2401 gggaagataa atccttgata gcagataata aagttgagac tgttagggca caaagagaaa
     2461 ctgtagaaag agaaaaagaa atggaaagaa gaagaataca agaagaagta gacaaagaaa
     2521 gatatattga aaaacatgga atggaaatat cagaaaatga tttaaaagtt aagctagagg
     2581 ctttagtacc tcaagaatat aaggggaaga aatatgaatt aaaagtagga aaatttaaaa
     2641 gatatagtat gtattttgat ttaactgtac aaaatgaaaa atttagtaat tctgaagaat
     2701 gtaaaaaatt tgtaaaagaa attgctaatg atcttaaaaa aattaaaata agcaaggcgt
     2761 actttaagtt tcattcaaaa gatgacggcg gtatatataa ttctgtttat attgattatt
     2821 ttagaaacat acaaaataat gtagataatg ttgaaaattt agaattcaat gagtttgaac
     2881 ttaaaaccga agaagaagag aagcgagaac aagagaaaat agaacaagaa aaaaacagtt
     2941 ataataacta cattcaaaac agagtggttg acccactaga tagaataaag aaactaaaag
     3001 agttgctaga ttcaggagca attacacagg aagaatataa taaaaagaaa aaagaattat
     3061 tagaatagat aatatagtaa gcacttacaa gtatgtaggt gcttttaaat ttacaaagta
     3121 ttccatttta attttatagt ttagatttta tgatataata aaaatataga agttttgcag
     3181 tgtgcgatat ttgttacaaa gtagggctta atacttgaaa tctaagatgt tgagggtgcg
     3241 tgataagtgt tatcaattgc actattgccc gctcactgca attttaagag tattgtatat
     3301 atgtaggtat tggaaatgct aagtttattt tggggtttta gattaactat atggaatgta
     3361 aatattacat gggtaaaaat tttgatgatt tttatgacag ttttagatta actatatgga
     3421 atgtaaattg tatagaatta agaatttttt taatactaat tatagtttta gattaactat
     3481 atggaatgta aatattacat ttaatttgca ttgctttgat gacttctttg ttttagatta
     3541 actatatgga atgtaaatgc gtacaaggtt gagaagctcc gcaatctcaa cgtcgtgttt
     3601 tagattaact atatggaatg taaatatttg aaaggaataa attttaaatg aatgaaagga
     3661 taggttttag attaactata tggaatgtaa atcagccaaa aacttcgaat ttgaactaaa
     3721 atgcgtgggt tttagattaa ctatatggaa tgtaaattct catgactatt gaataaatat
     3781 aataaacaag catacgtttt agattaacta tatggaatgt aaatttagaa tcaaaagcac
     3841 ttaatcctag ttctttcata gttttagatt aactatatgg aatgtaaata gctttttagc
     3901 ttcgtctact actttataag caaagcgttt tagattaact atatggaatg taaatgatga
     3961 acaataaaac aatcatctaa agacgaagaa agttttagat taactatatg gaatgtaaat
     4021 gttcctgttg tgttttttac taatcccatt ttatatgttt tagattaact atatggaatg
     4081 taaattgctg tgatttcact aataaaacaa ttaataattg tgttttagat taactatgtg
     4141 gattcaaaat taaataaaca aagaaagcac ttacaaatat gtaggtgctt ttattctgct
     4201 caaaattggt cggttgggta aaatcattag aaaaaattag taaaaacctc ttttctgtaa
     4261 ctcgttacaa tattattatt aatgtaacga gttacagaaa agaggtgaat aaaatagcaa
     4321 ctaaaagtag ggcagagtat atgaaaaatt gtcgaaaaga taaaagaggt tttagtgtac
     4381 ttttagacaa agaaaagtta gataaatttg atgaagtatt agaggaaaag aatctaacca
     4441 agaaagaatg gctagaagaa aaaatcgacg aggaactgga acaaaaggaa taaaaaataa
     4501 gggtcactcc caccgaccaa agtttgagta acccctatga tgtatactat cgtatatcaa
     4561 ttataatata tgtcattcct taaaaaaatc aattattaag gagtgtaata ttatgaaaaa
     4621 tgaattaatg atgtttgaag gaaaagagat tgaagtattt gaatttgaag gaagaatttt
     4681 attcaactca aaagatgtag caaattgttt agacataaaa aatgttaatg agaatataat
     4741 acttatgaat gaaaagcaag ttgtaaagtt aagaaattcg gatatcagta atactgacat
     4801 ccgaaaactt aataatgctg gagaaaattt tcttactgaa agtggagtgt ataaattaat
     4861 ctttaaatca agaaaagaag aagctgagag attccaagac tggataagcg atgaagtttt
     4921 accaagcata agacaaactg gtgcatacat aactaataat gtaatcccga aaagttgaga
     4981 gaaaaagcaa gtgagattga aaaattacag ttgcttataa cagtacatct atgttaaaag
     5041 aattgctaga tgatgcaggc tttgacaata aatcaaaact attaacagct aaaacattat
     5101 ataagaaagc agaaattgat ttaccaatag agataaacga agaagaacat tattttgata
     5161 caaaacaaat agcatctaaa ctgaaaatat attctaagag taataaacca gctcagatgg
     5221 ctgtttgtga gattattaaa aagattgatt tagaagatgg tgaagttaaa ggtgtttggg
     5281 aaattaatgg ttcttggact ggtactgtaa ataaatatac aaagagtgta atagataagg
     5341 tgagaacttg gatagaggaa aataatagac ctaccaagat tgcaggtgag aagaagaatt
     5401 attatgtatt ttacaaaatt gagtaaattt atcagttgta ttaaataatt tagtttagtt
     5461 ttggggggat taatacaatg tgtgagaatt tacttgataa tatgtatatt gaaaagagaa
     5521 aagaagaata tagaattaaa cttttgaaaa taagagaaac agatatagat atatataata
     5581 agttagaaag tatagtatat aaactttctg agaaaaaatt agagaaaaat aataaataga
     5641 catataaagc acttggatat tctactgttt caagtgcttg tttggtataa aatggtataa
     5701 tggagataga gaattatatt aaccaaaaaa tggagaaaat agagaagatg atttagttga
     5761 cgctttatca gtaggagtac ctgcaggtaa gacagaatct ctagtacgat tagttggaga
     5821 tggaataagt gatactcaaa aaagtttaat aaataaaaga gaatttagaa atataactca
     5881 aattggtgga aatggaaatg agaaagcatt tagtgaagta gaaaacttag ttaaatagat
     5941 tagaaggtag caactagagt tggttgttac cttctttttt tatactttct ctctctaagt
     6001 attttatttt tcatatcttc taatacatct aatgcagtag tataaaattg tatattattt
     6061 ctataaggta tagctattgg aagaatgccc ttgttaacta aaaaaataag aagtacaata
     6121 aataaaagtg tcataatcat tctaataaaa taattagtga tatctgataa atcaaatgtt
     6181 aagttaattg ttgtagaaaa tacaacagcc ataatagata tatacaaagc aaaaatgggg
     6241 ttactatttt tttgtaactg tcctttaagc ctagattgct ctatttctat atcttgaata
     6301 ctttttatgg aatccatttc actataatac ttataaactt ttaaataata ttttttatac
     6361 ttgtttaaag cttcttcatt atctttaagg tctttgtcac ttaactctga tttaacttcc
     6421 ccaaaatcca taaaatccct cctacaatgt tatataatat actttaattt atagcatatt
     6481 taagaactta tatcagcaat ttgtcgaacg attttagcta aaaatagagt ataatgatag
     6541 gcttattatt tataagatat tattttttat atagttatat atttctttct aattagataa
     6601 tataatgctg ttgttataat ggctactaat attattgcta ttattgtaat atctaatctt
     6661 cttgtaaata agcctattgc tatatactca agacctgtta catagcgata aagtacttct
     6721 agtttaacag tttttttctt atctatattt ccatatattg attctaattt attaaaatat
     6781 tttttactac aaaaagcagt tttaggttta attaaatata ttattgtagt tcctattata
     6841 actattattc caataagtat aagattgata tttccaaaat ccatagcaga tacctcctat
     6901 taaatttgct tttcttaatc aaagtatatc agtaacacaa gtacaaagag tatctaaaat
     6961 attacaaata ttaacaggat agataaaact atagtaaaaa ttgtaataat aaagcgaacg
     7021 aaaattatca tactaaaaca taagacatgt tataattgta ttagatagaa gattaacata
     7081 caccaaagca ctccttataa taaagagtgc ttattttttt gaaattcatc aatatataaa
     7141 ctatcaagaa ccttattcaa cacaccttaa aatcgattta aatagttctt tttgataaca
     7201 taatttatat gatataataa aaaaatagaa gtgtaccacc aatacacttc tatagctata
     7261 actaagcatt ctcttgctgg gggagtgctt ttttcatttc tccaaacaac attatttact
     7321 tcatcagtaa ttatcaaatc aatctcatta tctttcaaaa tctctgttgc agtacaaatt
     7381 agattatcta agtcaaaact tcgagttatc tctagttgtt tgttatttac ttctcttttt
     7441 agtatataag ttttaaaata catttatatc accccttgta tttactcttt tttctctaaa
     7501 aatatattga taagtaaata tgcaacaaat aaaaaaatct caatgacaca tagtgttata
     7561 gctataacta gaagtgattg atttttccat gcactataag ctatgatgaa aagaaatgta
     7621 aaagtaaata atgtataatt cataatttta actaagatat gacttgtttt cattgttaag
     7681 tacaagtctc tttcatctgt actatcgtgt aattcttcaa caatgccttt tcttgaaaaa
     7741 gcattacaaa aattaaagat tgctattaca attaaaagaa atgatgaaat taggaatctt
     7801 ttatctttta aaatgcaata aataactaaa ctagcacaac ataacacaca aactataata
     7861 cccactataa aacttcgttt actctttact ttcatgtttt ttatcctcca tttctttatt
     7921 ttcttttaaa caacatagtt cttcaatcgt tgtaccaaac acttgtgcca tacgataggc
     7981 aagcataaga gaagggctat attgctcttt ttcaatagaa ataattgttc tgtttgatac
     8041 atggactaaa tcagcgagtt gttgctgtgt tagacccata tttgttcgaa attctcgtac
     8101 ttttgttttc attccataac ctctttctat atgaagctaa cttcatgtga agttaacttc
     8161 ataatatcct aaaatgagta aaatgtcaat ctcattagat aagtattata aaacctaaaa
     8221 gaataatatg gtaaaaattg gatggaatta tttaccttaa ttctcttgtt tgtaaaactt
     8281 tattgatata gaatttaaca tatacataaa tcggcaaagg ggcgcgaatt atgaaaatta
     8341 gtaaattacc agaagcggaa ttaaaagtta tgagatatat ttgtgaagct aacaaggtat
     8401 taacttctag ggaattagtt gctgcaatgg aagaaaaata tgagtggaat gaatcaacta
     8461 catttacagt tttgaaaaga ttagagcgaa gagagttttt aagtacagag aaaataggca
     8521 aacttacaca ttataatata ctagtaaaag agaaaaaata tctaaggttt gaaacaaagg
     8581 aatttttgaa gaacatacat aaaaattcaa tttcaagttt aatgtcagcg ttacatggtg
     8641 aagacgacga agtggatgaa gataaattaa gtgaactcga agaatgtttt aagaatttga
     8701 aataagaaaa tgacaaattg atacataata aataaaatga aggtatgaaa ttataacttt
     8761 ttctaccttc attttgttta ttattctgaa catgttaaga aaatgaaata agtttgtatt
     8821 ttagttatat aaattttaat agaatatgta
//
LOCUS       DAMJZE010000159         8740 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJZE010000159 DAMJZE010000000
VERSION     DAMJZE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.163"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(90..605)
                     /locus_tag="PC541_003079"
     CDS             complement(90..605)
                     /locus_tag="PC541_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2840520.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(968..2038)
                     /gene="leuB"
                     /locus_tag="PC541_003080"
     CDS             complement(968..2038)
                     /gene="leuB"
                     /locus_tag="PC541_003080"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2840521.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2045..2536)
                     /gene="leuD"
                     /locus_tag="PC541_003081"
     CDS             complement(2045..2536)
                     /gene="leuD"
                     /locus_tag="PC541_003081"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2840522.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2551..3828)
                     /gene="leuC"
                     /locus_tag="PC541_003082"
     CDS             complement(2551..3828)
                     /gene="leuC"
                     /locus_tag="PC541_003082"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2840523.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3785..5527)
                     /gene="leuA"
                     /locus_tag="PC541_003083"
     CDS             complement(3785..5527)
                     /gene="leuA"
                     /locus_tag="PC541_003083"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2840524.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5651..5897)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6045..6491)
                     /locus_tag="PC541_003084"
     CDS             complement(6045..6491)
                     /locus_tag="PC541_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840525.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6512..7231)
                     /locus_tag="PC541_003085"
     CDS             complement(6512..7231)
                     /locus_tag="PC541_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2840526.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7349..8623)
                     /locus_tag="PC541_003086"
     CDS             complement(7349..8623)
                     /locus_tag="PC541_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2840527.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2651 a         1723 c          880 g         3486 t
ORIGIN      
        1 tatttttatc ttgtattttt attgtatttt tgaattttat tttttctaaa tataaaattt
       61 atgttttaaa atataaaatt tttatttaat caaagttcaa tatattccac tttttctttc
      121 gattcttcaa aaaaccattc ttcattagaa aaatcacctt ctgcaatttg tccaataggc
      181 attttcatat actttccagt tgctgtcact gctatatctc catttggtag aattatttct
      241 cctgtacctt caaataattt tctgttttct ttagttattc ttccaacaac ttttatagta
      301 gcatctgttg gtactggttt cttatatttc aactctaaag atactgttac tccccatatt
      361 tggtcatcat ttatacttac agctcttcca atagtctcat ccaatattgc agcagacata
      421 cctccatgaa ctctcccagg ataactttga tgccaatcct ttgtattaca aattgataca
      481 agctctccat tttcaagttc ataaaaatct gctttaagtc ctagctcatt ttgtgttcca
      541 caaactaaac aagatttact actattttgc ttcttcaaaa ctttatactt tgccaaaaaa
      601 tccatctcct aaccgacatt agtagtcgct taatattaaa taattctaaa atatattata
      661 taatattaat aaaatccctg caaacttcta ataataaaaa taggctactt caatatgata
      721 agtttaattt aaagtattat tttccaaaaa ataactttat caaaatagaa actcaaagta
      781 gatataaacc tctttttcgt tattaaaata gtaaaaatat atttgactta atataaaaaa
      841 gagtgtctca ttaactaata aagtttattt tgagacaccc ctcataaaac aaattatact
      901 acaatagatt tttactaatt ctacaagtta tatatttaaa ttatattatt caaattaaac
      961 tataattcta aatttcattt actattaatt cgcccatttc ccttgttcct acaacatttc
     1021 ccacaccatt atagatatca atagttctat aacctttatt gagtacactt tgtacagcat
     1081 cttcaataca cttagcttct tcttctaagt taaaactatg tcttaacatc atgcttacag
     1141 atagaatagt cgcaattgga tttacaatat cttttccagc tatatcaggt gcacttccat
     1201 gtattggttc atacatacca aaactatctt ctctaagtga tgctgatggt aacataccta
     1261 tagaacccgt aatcatagat gcttcatcag atattatatc tccaaatata ttattagtca
     1321 ctattacatc aaattggcta gggtctttta caacttgcat agcagcatta tcaacataca
     1381 tatgtgacag ttctacatct tcaaactctt tagctaagtc ttccattata cttctccaaa
     1441 gtcttgaact ctcaagcaca tttgccttat ctacacttat aagtttttta tttctatttc
     1501 tggctatttc aaaagctctc ttaccaattc ttcttatttc attttcgtta tatatttcta
     1561 catcataagc attttcgata ccatcaatga tttttcttcc acgttctcca aaataaatgc
     1621 cccctgttaa ttcccttaca acaacaaaat caatgccttt ctcaactata tccgttctaa
     1681 gtggtgaagc ttcttttata ctttcataca ttgtagctgg tcttaaattt gcaaataaac
     1741 ccaatgactc tcttaatttt aaaagaccag actctggtct tttatctccc ttacatttat
     1801 cccactttgg tcctccaaca gcaccaagca atacagcttc gttttttcga caagtttcaa
     1861 cagttttatt aggtagaggt tctccagttt catcaatcgc gcatcctcca gctaatacat
     1921 attcacaatc gaatctatga ttaaatttac aacaaatttt atttagaacc tttatacctt
     1981 catctataat ttccggtcca acaccatcac cttttatgac tgctatatta caattcatac
     2041 ttcactaccc cctatttgct tttattgagt taattaagcc ttcatttaat ataatatttt
     2101 gcataaactc aggaaatggt tctgctttat attcttttcc ttttgttata ttttttatca
     2161 cacctgtaga aaaatctact tctatattat ccccttcatc tatgttattg gcagcttctt
     2221 cacattcaag tataggaagc cctatattta tagagtttct aaaaaatatt ctagcaaaag
     2281 tagaagcaat cacacaggat actccacttt cttttattac aattggtgca tgttctcttg
     2341 atgaaccaca accaaagttt ttagttgcaa ctataatatc tccttttttt accttagaaa
     2401 taaatttatc atcaatatcc tccatacaat gcgttgctag ttctttataa tcagctatat
     2461 ttaaatatct agctggtatt attacatctg tatctatatt gtcaccgaat ttaaaaacac
     2521 ttccattggc aatcataaat tttacctccc ttaaattatt tctgatggtt ttgatatttt
     2581 tccagttata gctgatgctg ctgctacagc tgggcttgca agatacacct ctgaatcaac
     2641 atgccccatt cttcctacga aattcctatt tgttgttgaa atggattttt ctcctgctgc
     2701 aagtataccc atatgtcccc ctagacatgg accacaagtt ggtgtagaaa ctgctgctcc
     2761 tgcctctata aaagtggtaa tatacccttt ttctatagct tcaagatata ctttttgagt
     2821 tccaggaaat atcataactc taactccatc tgctactttt ttgcctttca ttatctcagc
     2881 tactacttca aggtctgata ttcttccatt tgtacaagag cctattacaa cttggtctat
     2941 ctttatatcc ttatctatct catctactgt tctagtattt tcaggaagat gaggaaaggc
     3001 tactgtttct tttagttcag ctaaatctat ttcgtatact tcatcataga ctgcatcttc
     3061 atctgcttca tatatattta catcttttgt catagttgaa cacttatgtg aatttatata
     3121 ttccatagtc ttatcgtcta ctggaaaaat tccatttttt gctcctgctt ctattgccat
     3181 attacaaata gtaaatctat catccataga taagtattct actccatctc cacaaaattc
     3241 cattgacttg taaagtgctc catcaacacc tatctcccct attatatgaa gtattatatc
     3301 tttaccactt atccatttat ttggtttatt cttcaaaaca aattttattg ctccaggaac
     3361 cttaaaccaa cattttcctg tagccatacc tgctcccata tcagttgaac ctacaccagt
     3421 agaaaatgct cctaatgctc catatgtgca agtatgagag tcagcaccta taattacatc
     3481 tccagcagtt actatacctt tttcaggaag aagaacatgc tctattccca tatctcctac
     3541 atcaaaaaaa tttacaactc cttttgattt tgcgaaactt cttgtaaatt tacattgttc
     3601 tgcactcttt atatctttat taggtgtaaa gtggtctaaa accatcacta ctttagtctt
     3661 atcataaaca tcttcaattc ctaatttttc aaattctcta atggctactg gtgatgttat
     3721 atcattacct aaaactacat ctaaatctgc ttcgattaat tgccctttct taacttcatt
     3781 taatttagca tgtttggcta atattttctg cgtcatagtc atccccattt tttatttcct
     3841 cctcaatata acttttattt attgcattca taagtgcatt aatagctgct gtatttatgt
     3901 tttccgatat acttacacca aaatagactt cattattgtg ctctattcct acataagcta
     3961 cccctcttga atgtgaccct tcctctaatg catgttccca gtaatatcta aatttataat
     4021 ctttcatgcc acattgcttt agtgcattat taaatgaatc tacaggtcca tttcctatac
     4081 caacaatcct ttgttcattt cccatataac ttattcttgc agtcatctca atcatgttag
     4141 tatcattaga atcatcattt tcatagttta attcatccat tgattttatt ttatacttct
     4201 taactttata agggctctca acttcaatat attctttctt aaacaaatta aatattgctt
     4261 cactactaag ttcttcccca attctatcag attccatttt gactacattt ccaaaatcag
     4321 aatgcatatt cttaggtatc ataaatccat aatctgtttc cattatataa acagctcctc
     4381 cttttccaga ttggctattt atccttataa tttctttgta ttctcttcca atatcatgag
     4441 ggtctattgg taaatatgga acttcccagt aatcattatc tctgtttttc attgctatca
     4501 tgccttttct aatagcatct tgatgtgaac ctgaaaaagc acaatgtaca agattacctg
     4561 catatggatg tctatcatgt accataagtt ttgtacaatc ttcatatata cctattatct
     4621 tatctatatt gctaaaatct agctctgggt ctattccttg tgtataaaga ttaagtccca
     4681 catttactat atccatattc ccagttcttt caccatttcc aaatagtgtt ccttcaagtc
     4741 tatcggcacc agccaataat ccaagttcac ttgcagccgt acaagttcct ctatcattgt
     4801 gagtatgcaa acttagtata atagaatccc tacaacttat atttttacaa aaccattcta
     4861 tttggtctgc atatacgttt ggtgttgcca tttcaactgt tgatggaaga tttattatta
     4921 cttttttttg agtagttggt ttccaaacat ctatcactgc ctcacatatt tcaagtgcat
     4981 aatccatctc tgttcctgta aaactctctg gtgaatattc aaaagtaaat tctgtttctg
     5041 gatatttttt tacttcttca ttaaataatt ttgctccttc aactgctata tctattattt
     5101 cttgtttact catattaaaa accacatctc tttgaagaac agatgtagag ttatataaat
     5161 gtataattgc ttttttacat ccccttattg attcaaatgt tttttctata agatgagccc
     5221 tagattgtgt aagtacttgt atagttacat catctggaat catattctca tctattaatt
     5281 ttcttaaaaa tgtatactct gtatttgatg ctgatggaaa tcctacctct atttctttaa
     5341 atcctgtatc aataagcatt ttgaacattc tgactttttc atttacactc ataggtgttg
     5401 gcagtgactg atttccgtct cttaaatcga cactacacca aattggagcc ttaaatattt
     5461 cattatctgg ccatttacga tttactattt gcattttatc atatttttta tactttccac
     5521 acttcatgtt atttccccct ttttctgttt tttgtattca gtatacctca tatcgacgca
     5581 atcctgcaca attcataagg ataaatgtca attttatcat tagaataaaa aaaacctcat
     5641 ctctaataaa attagagacg aggttttgct cgcggtacca ctctatttag ctttctaaaa
     5701 agctccctta gtaatagcca atacaataat tataatgact atatttccta taacgtggaa
     5761 ttactccgat tattctaaga tttaaatctt agaaaacagc tcctagacaa gttcagaact
     5821 atcaactact aaatcacacc atcctttagc tctctgaaag tttcattgtt cttactactt
     5881 ctattcatca cttttttaat catttaaatt ttctaaatat tttgatttaa tagttattat
     5941 atacacatta aaatgtattg tcaacacaaa aaataattat ctcaattttt tatttttgat
     6001 aagcaattct ataaaacatg ctaactattg aaaacagtat gatattatgc cttaaacttt
     6061 ttcatctttt catctatgat attcaataat tttttttcat cttccaaact caatgccctt
     6121 aatctaattt tataattaga ttcgtcagtg tatatattta atatattttt tactacttct
     6181 attttttcta ttatcttata attaaacttt tgactttgtt tagattttat actgaatata
     6241 cattgattat ttattaatat tgaatgatta tcgagagtaa tatatacttt tccctcatat
     6301 ttatttgaaa aatcacgacc ttgtttaaat agaaaaactg ctgttattat acataaaata
     6361 tataatattg tcgttgaata tataaaaatt gtagctaaaa ttataagtgc taaaaccata
     6421 tatatagtca ttcctttttt aaaataacca ttgtcataat aattcatata gataaactta
     6481 tttttactca aattttttca cttccaagat tctaaaaatt tatatcaaaa aattctataa
     6541 tttcatcttc atgataattt atgactaatt catctggaaa atctactgct tctaaaactt
     6601 tttctgcatt ttcaaattct cctatagaat aacatatatg agagtcagta ccaagaatta
     6661 ctcttgcacc atatgtttta cacaatgtca acatatgact tacattttcc caagtacccg
     6721 ttctatgaga gtttgaactt aatgatgaat tatttatttc taaaaggata tttttatctt
     6781 tagctttttt tactataggt tcataatcta aaggatacct actgtcatct ggatgtccaa
     6841 ttatttttac cttaggttta tccataactt ttaaaatagc atttgtattt tcttcttttg
     6901 taccagaagc tatacaaggt ctatgcaaac ttgcgattat ataatccaaa tgttgcaaca
     6961 tatcttcttg aacatctaaa ttaccattat aatccattat atttccttct attcctctaa
     7021 gaatctttac gccttgcact tcacgtggaa gcaaatgcaa attataaaaa tagaatggat
     7081 gtggtcctcc tggcatatta ggtccatggt ctgatattcc taagtatttt atattcttct
     7141 tactcgcctc ttcgacattt tccttgaaag tactatgtgc atgtccactt attagagtgt
     7201 gacaatgtac atccataata gactttttca ttaaatttcc tcctaatatt ttacataaat
     7261 tcgtgaaaat agctaaaggg ctatttcaaa actaaagctg tcataataga cagtgtaacg
     7321 ttttaaaaca gccccatttt caattatttt acacttctaa atctttaaat ttatctttaa
     7381 ataattctcc aatacttcct aaagattcct ctacttctag gtattctgtt atatcctctt
     7441 ctggctctct agttgcttct tttattgaaa ccaacattct cttagtcttt ggtttaaaat
     7501 caagaatctt aactttaacc tcatctccaa tattaacaac attagtaact ttagccactc
     7561 tagtatcact caattcagat attggtaaat acgcatctac accttcttta atttgaacaa
     7621 aagccccttt ttctattatt ctaagaactt taacattgac aacatcatca atttgtactt
     7681 cttttgctat taaatcccaa gggtcattat ttatgtcttt taatgccaag gcaatttttt
     7741 tagtctcttt atttacatcc aacacataaa cttctacttc ttgtccctct gaaacaacat
     7801 cttcaacact ttcaactctt gtccaagcta aattatttat atgtgctaat ccttcaatcc
     7861 caccaatatc cacaaatgca ccataaggca ttatctttgt caccttacca tctctttttt
     7921 gtccaacttc aagagaatta aataagtctt ctttagctgc tttgatacga gctttttctt
     7981 cttctctttc ttgcttaatt cttgctcttt cagcttcttt ttctgcctta atttgagctc
     8041 ttctttcttc tctctcttta tttatacgct cttgtaatac atctctatga gatgctacaa
     8101 gtctattttt tctaacatcc aattctttta tataaacttc taagttaaga ccaacaaatt
     8161 tagatgtatc tgtaataaat tttgtatcaa gttgagaaat tggtataaaa aatgtatatg
     8221 tattgtaatt agcaaataca cctttatcta tattcttttc aacatttaca gttattattc
     8281 tatgttcatc ataagccttt tgtaactcgg caaaatcatt tcttttgtca agttgtaatt
     8341 tagataaagt tattgtacca tctttttgag atacttttgt tattacagca gttatttcat
     8401 cacctatgtg gtatatatct tctatatatt gatttttttc tatgttcaat tcacttatag
     8461 gtattattcc atcaaaccca tagtctaatc ctaattgtac ttcatcattt ctcactgcag
     8521 ttatttttcc agttgtcaat tctccaactt tcactttgct gaatacttgt tcttgctggt
     8581 caagtaattc ttgcattgtt aaatcatttt ccattataaa catcctttcc tcttattgat
     8641 tatcatatct gtaaatatta taccattaat attgcaaata cttacatttt tcttactata
     8701 atttttaaaa taaattcaac ttcatatatt tatacattat
//
LOCUS       DAMJZE010000160         8675 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJZE010000160 DAMJZE010000000
VERSION     DAMJZE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.164"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            100..558
                     /locus_tag="PC541_003087"
     CDS             100..558
                     /locus_tag="PC541_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2840528.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(675..1412)
                     /locus_tag="PC541_003088"
     CDS             complement(675..1412)
                     /locus_tag="PC541_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2840529.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            1629..3305
                     /locus_tag="PC541_003089"
     CDS             1629..3305
                     /locus_tag="PC541_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2840530.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            3511..3711
                     /locus_tag="PC541_003090"
     CDS             3511..3711
                     /locus_tag="PC541_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2840531.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            3765..4928
                     /locus_tag="PC541_003091"
     CDS             3765..4928
                     /locus_tag="PC541_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2840532.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5139..5561)
                     /locus_tag="PC541_003092"
     CDS             complement(5139..5561)
                     /locus_tag="PC541_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2840533.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(5582..6418)
                     /locus_tag="PC541_003093"
     CDS             complement(5582..6418)
                     /locus_tag="PC541_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2840534.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            6716..7312
                     /locus_tag="PC541_003094"
     CDS             6716..7312
                     /locus_tag="PC541_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2840535.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            7342..8061
                     /locus_tag="PC541_003095"
     CDS             7342..8061
                     /locus_tag="PC541_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2840536.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            8063..8602
                     /gene="scpB"
                     /locus_tag="PC541_003096"
     CDS             8063..8602
                     /gene="scpB"
                     /locus_tag="PC541_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2840537.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT         3414 a          977 c         1411 g         2873 t
ORIGIN      
        1 aataaattaa atttatttaa atgtcttgac aaaaaatgaa ttatcaatta caatattatt
       61 taataaagat tatcaattga aaatagtaag ggtgatgtta tggcaaatac aatggattta
      121 ttaaaagaca agttaaaaga aactggtttt aagattacgc cacaaagaag agcaatagtt
      181 gaaatattgc ttaaacatga ccattcacat ctaagtagtg aggaaatcta tgatttagtt
      241 agggttgact gtcctgaaat aggcttagct actgtatata gaacaatgca gctattggat
      301 gaaataggat taatatcaaa actaaactta gacgatggat gtataagata tgagattagt
      361 ctacataaag aagactgtca taatcatcat catcttattt gtaaaaattg tggaaagata
      421 atggaagcta aagaagacct tttagacaat atagaaaaag agatacaatc attgtacaaa
      481 tttaaaatac ttgatcatga tgttaaattt tatggactat gtgacgagtg taatggagta
      541 agcgattcag aagaataaac ttctattgct taatgtcgat ggaagaatag tatagaactt
      601 ttatattcta tgctattctt ccatttttta ttataaacca tctataaaat gcaaatatac
      661 agttttcaag atgcttatag ttttaatatt gaaaatataa ttaatcctat aagtacaaaa
      721 atgaatgatt tttctatgaa attactaaat ggtcctccaa cagttaaaaa acctaaagaa
      781 attttctttt cgcttagcgg ataaaaaatt ggtattcctt gctttgtaaa taaatctcct
      841 aaaaacatat gacttgcata accaatcgta ccataaaaag ataaattatt aatattagtg
      901 attaattcta tttgttttag taaaaaatat actattatag ttgcaaatat gctatgagaa
      961 aaacttctgt gctttaacca gggaaaaatt gacaacatta gacaaaaaat tgtaaaatat
     1021 atttcaactt ctatgatgta taagcatata gctaatagta taaatattaa tgatagaaaa
     1081 acttttctta gaaatgtgtg ggttagtttt gcctcaataa ttataattgc aacaaatgta
     1141 attattgcgc ttaaataaaa attatcattt atatttattg aaataaagaa aataataata
     1201 ttaacagcat atataaaaat tctatatatg tatttggata cattgttttt tagaatgaaa
     1261 tttgagacca tagaattaga tttatccaaa tcgggtagta cagcaaaagt agctgaaact
     1321 aaaatatcaa ctaaagatat aggaatttta aatattaaag atgtttggat agctgttaag
     1381 attcctattg cacaatgagt tttaccacgc ataaatcctc ctaaattatc cgatattaaa
     1441 tcatataata tttagaggat atcaaaataa aataggtttg tcaaaataat acgttaaatg
     1501 tattttaaat atagcttaaa atatgatacg aattatggta caataagaat aactggtagc
     1561 gagtgcgtga tttgatatta tttatgattg tagaacgtgt taaaaaatta aatagacaga
     1621 aggagatgat gcaattgttc aaaaagaaca ccaacaagat aaaggtaatg gccttgggtg
     1681 gacttaacga agttggaaaa aatatgactg ttgttgagta taaagatgaa ataattgtta
     1741 tagatgctgg acttagtttt ccagaggatg aaatgttagg agtagatata gttataccag
     1801 atataactta tttagtaaaa aatagggata aaataaaggg tatatttatt acacatggac
     1861 atgaagacca tataggagct cttccatata tattaaagaa aataaatgta cctgtatatg
     1921 gggcaagact tagtataggt cttatacaag taaaacttaa agaacataaa atgaataatg
     1981 ttaagttaaa tgttataggt cctagacaag ttataaagct tgataatatg gaagtagagt
     2041 ttttgaaaaa taaccatagt ataccagatg cttactctat tgcaatacac acagaccaag
     2101 gtataatata ccacacagga gattttaaga ttgacttgac tcctattgat ggagatgtta
     2161 tggatatgca tagaatctgt gagttgagca aaaaaggagt tcttttaatg ctggcagata
     2221 gtactaatgc agaaaaacca ggatttacta tgtctgaaaa gactgttggg gtaggtcttg
     2281 acgaattatt tgcaaaagga aatggcagaa gaattatagt tgctaccttt gcatccaaca
     2341 tacacagatt acaacaaata ataaatactg ctgaaaagtt taatagaaaa gttgctatat
     2401 caggtcgctc aatggtcaat gtagttggag ttgcaaaaga attaggatac ctagatattt
     2461 ctgatgacat gcttatagat ttaaatgata tatgtaagta tgaagatagt gaattagtga
     2521 taataacaac tggttcacaa ggtgaaccta tgtcagctct tgctagaatg gctttttcag
     2581 agcacaaaaa agtagaaata aagagtggcg atttagttat aatatctgct catccaatac
     2641 caggaaatga aaaattaata tcaagagtaa tcaatttctt atttgaaaaa ggagcagaag
     2701 tagtatacag tgatatagct gatatacacg tttctggaca tgcctgtcaa gaagaattaa
     2761 aacttataca tgcattagtt agaccaaagt tttttatgcc agctcatggt gaatatagaa
     2821 tgttaaaaag acatgctgaa atagctgaac agcttggtat ggataaagaa aatatttttg
     2881 tcatgcaaac aggagatgtt ttagaattag ataaaaattc tgctaaggta gcaaaccgta
     2941 tacaaacagg aaacatatta gttgatggtc taggagtagg cgatgtaggt aatatagttt
     3001 taagggatag aaagcattta tcagaagatg gtcttatgat agttgtagtt actatttcca
     3061 aggatgaggg taaggtctta gcaggtccag atataatttc cagaggattc gtatatgtaa
     3121 gagagtcaga agatttaatg gatggagcaa aggatataat taagaatgtt ctaaatgaat
     3181 gtgaagagaa aaatattaaa gaatgggctt atttaaagaa taatattaaa gaaaacctaa
     3241 aagaatattt atatcaaaaa actaaacgaa atccaatgat acttcctatc ataatggaag
     3301 tataatataa aaaaactata aatttgaaaa ttagacctct tgtaattttt aaagtaatat
     3361 caaaaatcca attgatatta tcttaagaat tacaagaggt ctttttaata ctcattttaa
     3421 aaaaatatat attacataaa ataccaatta taacaatctt ttattagtaa aaactactta
     3481 tatataaatt ttactgatag gaggattctt atggatgatg tttcaagaca aaatgctata
     3541 aaagctttaa aacaaacaaa aatggaaata gcaggagagt atggaatgaa ttatgaagat
     3601 gcttttgaaa taatagaaaa tgcgtctaat aaaggtgttt tagaaggtta ctttaaaaag
     3661 cttgaaaaga aaaaaaattt aggtcaaggt atatctaggc atttagaata aaaaaagtta
     3721 aatgtgagaa aataagttta aataaacttt aagaggtgac atcgatgaaa aaattagcta
     3781 gtactgcttt ggctatatta attgcgctta ctcctttaag cttttctttt gcaaataata
     3841 aggaaaatgc tgatgccaac caactaaata tatcttcaaa atcagcaatt ttgatggatg
     3901 ttggtagtgg acaaatttta tatgaaaagg atgctcataa aaagcttcct cctgctagtg
     3961 taactaaggt aatgactatg ctacttatag tagaagcttt ggattcaggc aagattaagc
     4021 ttgatgatga agttcaagta agtgaaactg catctagtat gggtggtagt caaatattcc
     4081 tagagccagg agaaactcaa aaggttgata cattattaaa aggtatagct gttgcttctg
     4141 caaatgatgc ttgtgttgct atggctgaac acttagctgg aagtgttgaa ggatttgttg
     4201 atagaatgaa tgctaaagca aaagagttaa atatgaatga tactcatttt gcaaatacaa
     4261 atggacttcc agttgcaaac cactatacat ctgctcatga tatagcttta atgtctagag
     4321 aactattaaa acacgaaatg ataagtaaat acttaactac atggatggat aaggttgtag
     4381 tagggaagaa acaagttaca gtaggtcttg caaatactaa taaacttata aagcattatc
     4441 aaggagcaac tggtgttaaa actggtttta ctcaagaagc aaaatactgt ttatcagcat
     4501 ctgcaaagag aggtaatact catctagttg ctgcaacttt aggagcagag acttctccag
     4561 aaagatttaa tgatgcctct agccttttaa attatggatt tgctaattat gaaagtgtta
     4621 agttatgtag taaaggtgat aatatagcaa cacttacatt ggataaggct gatgaaaaca
     4681 aagttaaatt ggttgctaaa gaagatttaa atgctcttat taaaaaagga agttctaaag
     4741 aatttgagaa gaaaattgaa atagtaaaaa atcctaagat gccaattaaa aagggaactg
     4801 ttttaggaaa aataaagata tgtaaagata aaaaagtaat aggcgaagtg gaattgataa
     4861 atactaagga tataaataag gcaagctatc tacaaatgct tcaaagaata attgataata
     4921 tgatataaat aattatcaga tgaaatataa ttataataca caaagatatg attataatac
     4981 acataaaaaa tacccttatg taattatttg ccataggggt atttttattt accatataga
     5041 agtattttat aatcttatga tttttataga aatatagaaa agtttatgta tcttaaaata
     5101 tcatttgatt taatatcata tatagggttg tttaacaatt atctaagtaa aggcatacta
     5161 ccagtaagtt tattcacttt ctttttattc ccataaattg ctatcccaaa atactgtaac
     5221 tcatcttctg gaacactagc aatcttttgt gtaaattctt cataaagatt gcaactttgt
     5281 gctacatctg aaaaatctac aacagttaaa tcgttaaatt ccaaagtata taatttttta
     5341 cgtaaatctt ttattatttc tttgtttcct tttaaaatag gtacaggaat gtcaataatt
     5401 cctaagtggc tattacctgt tttgtcaatt acatctgggc caacagtttc tggggcatgt
     5461 tttcctagtg taattcccat tatagctgct gtattggata taattcccat tggtaaattt
     5521 tcgtctatta ccatgacaca ttttgaattt ttagtttcca tttttatttc tcctttttac
     5581 cttatttaaa gttgtttgaa tcatcattta taaatatatt ttgatactgc tttggtgtaa
     5641 gaccaataaa attcttaaaa aagtttgtaa agtgactttg gtctacaaat cctgtttgta
     5701 tcgcagcatc tataggttct acgccttttt ctaaaagctt tttagctttg tttacccgta
     5761 tattttctag gtatctatat ggtgtaatac cttttaattt cgtaaaatta cgaagtagag
     5821 agtacttatt catacctgct atagtgctta attcgtcaag tactatatgt tcagcataat
     5881 tattttctag ataatcacat actgcttgaa tttctatatt tgtattttcc aagttagatt
     5941 gcaaatttgg ctctgtatgt tcttcaatta attgttcaat taggaagaaa aaaagctctt
     6001 cctttttaaa atccaattct tcttccatga ttatatagtg taattcttgt agtagaggta
     6061 ctagctcact gcgaaatata acaggttgat taaattttgg taaataattt ttacctgtaa
     6121 tttcaaagac agtttttttc attatttctg gcttaatatt aatacatctg taatcaagta
     6181 ctttatcatc tatttgttca caagtatgac tatcaaaagg gttaaaaagt agcaaatctc
     6241 cagtacttgt ggtatactct ttatttttac aagataaata tctttgacct ttttctataa
     6301 aaccaattac ataatgttca tgaaaatggt ttgggaattt ttgcattatg cctttaaatc
     6361 tatatgcttc aatttttaat tccctatcaa agcatactgt gcgttcttct tgtctcactt
     6421 aggtaacctc ctttcatgtt atacaaagtg taacacatat ttttttatac ttcttgtata
     6481 atattgacta agagacaata atgtcaattc atgtcaatat aatattacac ttacagtagg
     6541 tgtaattatt atattttcac aatgaatatg atataataat aaaagttata aaataaaaag
     6601 caaattttaa ttttatgcct agaagttaaa attattataa tgtaaaataa ttttgattat
     6661 aagctattta attgttataa taatattaaa aatttaattt aggagatgta cattaatgtt
     6721 aagtatgaat ataggagaaa tagtagcaag tttagttggt atagctatgg caatatcaat
     6781 acatgagttt ggccatgctt attcagctca cttattggga gatgatacag ctaaagctta
     6841 tggtagaatg acattaaatc ctgtaagaca tgttgaccca ataggtcttt tggctatgtt
     6901 tatccttaag atagggtggg caaagccagt accagtaaat ccaaataact ttaaaaacta
     6961 taagatagga aacctgatag tttcacttgc aggcgttttt tgtaatgttt tgactgctat
     7021 actgtgtgta attatcaata agtatgtgca tatgtatgct ataaatacaa tagctgaata
     7081 tgtgttttta tatagtattg gatttgcagc ttttaactta cttccgatac ctccacttga
     7141 tggatgggga gttatatcta catttgtacc atataaatgg aatgaatatt tgtataaatt
     7201 tgaaagcatg agttatataa ttttattaat tgctttattt actggtatgt atagaataat
     7261 attaacacca atatatgcag tgttgctaaa aatagtatat ttatttgttt aatataataa
     7321 acagattaaa gagggattga tatgaagtat aatatacaat tacaagtcta tgagggacca
     7381 ttagatttac tttatgattt aataacaaag cataaaatag atataaagga tatatccata
     7441 atagatataa ctaagcagta tctaaattat ttgaaaatgc tagataaaat ggatttagag
     7501 ataactagtg aatttataac tatggcatca aaacttttag agataaagtc taaatatctg
     7561 ctttataagc aaaaagatga agaagaagac ccaagaatag aacttatgga aaagttagaa
     7621 gaatatagaa agtttaaggt agcatcacaa gatataaaag aaaatataac ttatgttaat
     7681 gagagatttt atagaaataa agaagagata attattgatg acaatgtgga tttagaagat
     7741 atatcaatag aagcaataaa aaatatactt ccatacatat ttaaagttaa aacatcacag
     7801 atagaaaatg ctaatgatga gaaactagat aaaatagtaa ggaagaaaat aatatctgta
     7861 gaagaaaaaa tattatatat aagagatatt ataaaagaga agattgaagt tacttttaca
     7921 aatattataa aatcatatga aaatgatgaa ataatagcta cttttttatc tatattagag
     7981 cttataaaag aaaaagaaat agtcgtagta caagatatat tttttgatga tattctaata
     8041 agaaaaagtt cggagtgtta aaatgaaaag agaagatata aagtatatta tagaatctgt
     8101 tatgtttgca tatggtgaac caataagtat aaaagaatta aactatataa taaataaaga
     8161 actttcatca aaagaaatag agattatgtt aaatttactt attgaggagt acagagaaca
     8221 aaatagaggt attcaaataa tcaagttaga gaataagtac caaatgtgta caaataaaga
     8281 ctatgcagaa tatataaaga agattattga accaaagaaa aagaaaagtt tgagccaagc
     8341 tacattagaa actcttacta taattgccta caaacaacca ataacaaagg tagaaataga
     8401 ggatataaga ggagtaaaat gtgataaggt attacaaact ttgtttgaaa atgaacttat
     8461 aagagaagct ggtagattga ataagatagg gaaaccaata atttataaaa caacagatga
     8521 atttttaaag ttgttgaata tagaaagtct tgaagaattg ccacctatag aaaattatca
     8581 agaagtggca acaaatgaat aaaaatataa aatcacaata atttagtatg taattttaaa
     8641 ttttaaaaga tttaaaatgc tctaagttgt tgtgt
//
LOCUS       DAMJZE010000161         8644 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJZE010000161 DAMJZE010000000
VERSION     DAMJZE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.165"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            128..1909
                     /locus_tag="PC541_003097"
     CDS             128..1909
                     /locus_tag="PC541_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2840538.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1991..2521)
                     /gene="lepB"
                     /locus_tag="PC541_003098"
     CDS             complement(1991..2521)
                     /gene="lepB"
                     /locus_tag="PC541_003098"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2840539.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2561..3385)
                     /locus_tag="PC541_003099"
     CDS             complement(2561..3385)
                     /locus_tag="PC541_003099"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2840540.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3642..4550
                     /locus_tag="PC541_003100"
     CDS             3642..4550
                     /locus_tag="PC541_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2840541.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4694..6139
                     /locus_tag="PC541_003101"
     CDS             4694..6139
                     /locus_tag="PC541_003101"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2840542.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6450..6902
                     /locus_tag="PC541_003102"
     CDS             6450..6902
                     /locus_tag="PC541_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2840543.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(7237..8433)
                     /locus_tag="PC541_003103"
     CDS             complement(7237..8433)
                     /locus_tag="PC541_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2840544.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT         3416 a         1105 c         1259 g         2864 t
ORIGIN      
        1 ttttttagca gttttgtatt tatttgtatt tattttgaat ttatttacta aatttgccta
       61 taaaaagtat atataaaaat atagttcaaa atggtataat taagtataac agaaggaggt
      121 aatgtaagtg agccaataca taaatataaa ttctcagtta gacgccctaa aattgaagga
      181 gttcgagaaa atattttacg aaaatatcaa tgaggacgtt aaagtagtgc caaaagttac
      241 tccttttaaa ggaataaata ccgatttgtt gtatgttaaa gatggaagaa ttttatttat
      301 aaaatttatg gatacaacag aagatatatt ttttatttta gaagaagagt tacttgaagt
      361 tatgaatgag gaatatgaac ttttgaaact aaaaatggga caaaagaata gaaatataag
      421 ttataactac gtttacatta tgccatacgt tgaagttgag gaaacatatg aatttgaaga
      481 atttgttaat aataatataa tagataaaaa taagcttcaa gatataatga ataagggttc
      541 gctggaggaa tatcttaatg atgagaatga tgaaataaac ttaaatttat ttttactaga
      601 catatgttca gaatactata taataaatga taaacttcat ctaaatgaaa aatttaaaaa
      661 aatttcattc tacaatgatg actataagta tacagcaact atgatggaag acgttcaaat
      721 taaagatgtt gtttctatta aatatgggaa tacacttatt gaaggtggtt ctggtatagg
      781 caaaacagca attatgcttt ctagggctat aaaattagca aaagtatatc cacatcataa
      841 attagtcata tttacacata caaaacaact gtgcaatgag cttcgagaga ggatagagtt
      901 attatacaaa gataataaca atttagaagt tcacacattt agttctttta tatttaaact
      961 agctaaaaaa ttcaatttaa ttgtagatta taacatgtta aaaaatgatt acgaaaagac
     1021 ctttaataat cttgtaaaac aagcacagaa cattataaaa aataaaaata tgttcaaggc
     1081 tatttttatt gatgaagcag aaagcttttt agagtatgaa atagatttta taagggagtt
     1141 tttatataaa acaaaattca tatttaatgt gtgttcttgt aactccttga atatctctaa
     1201 tagattgaat atatttaaaa aattatataa tggtatagaa tttgatgata agattatctt
     1261 aagtaaaaat tatagacaag ctaaagagat tgtagatttt acaaataaat tttcaaataa
     1321 ttctaactca tatatcaacg aattaagacc aaatacagaa tttagtactt tcttttatac
     1381 taaagcactt agaggtggaa ataagtctgt tgatataata aaagtaagtg accttgatga
     1441 ccaaataagt tctgtaatct gggaaattga gtatttaata agtaaaaaag gtcttgatta
     1501 ttctgaagtt gctatcgttt atccttacaa taagaagaaa ttaaaaagtg gaaaaaccat
     1561 ttactttcaa tatatgttaa aaaaagcatt ggaagaggca aaaattcctt atatatgtgc
     1621 agaagataat ttgacaaata taagtaagaa ggtaggaact acaatagcaa atatatatgc
     1681 aataaagaat ttagagtata aagcggtaat agtatgtgaa ttagagatgt tatacaatca
     1741 aactataaat gatattgaac aagattatca agtaaatgac tttgtaggag atttaaataa
     1801 ggtttattta gctatgagta gagcaacaga ctacctaagt atagtaacaa cttttaatga
     1861 agaagcaagt gatataataa gacttattac agaatcaaaa gatatttagt aaaaaaatat
     1921 ttgtaattta agaaaataaa aagagactga gatttgtaaa ataaacttta ccaattcagt
     1981 ctcttatttt ttagtctact ttcccaatat tatcaagagg taatagtctc accataacct
     2041 taccaagaac ttcatcttca tcaaccatac ctacatcagg gaatctacta tcattactat
     2101 tttctctatt atctcccatt gcaaatagtt taccttttgg aacaacagta tctatatctc
     2161 cagaagtata gttattgtgt atatatggtt catttaataa ttttccattt acatacactt
     2221 tagaatttga tatttttatt ctgtcacctt cagtagctat aactcttttt attaagtctt
     2281 ttttctttcc tgtttctcca tcaactaaat tggttttaaa aactacaata tcgccttttt
     2341 caggtttacc aaccttgtat gatatcctat ttaaaatcag ataatcttta tcatctaaag
     2401 taggatacat tgattctcca cttacaatag aaggtcttat aaattgtaga attacaaatg
     2461 caagtacaat agccatagct attgacttaa tccaatcaaa tatttctttt ttaacactca
     2521 taacttttct ccttatcatt tgttattaaa ttagaataca ttataataga aagaaataag
     2581 caagaaccac tatacccaag actattctat aataaccaaa tactttgaag tcatgttttt
     2641 tgatatagtc cataaaaaat ttaataacta caattgaaac aacaaatgct actacagaac
     2701 caacacctaa aataagccat tcaaatccag taaatccaaa tccagctttt agaagtttta
     2761 atgcactagc tccaagcatt gtagggatgg ctaagaagaa tgaaaactct gctgctacat
     2821 atctagaagt accaagtaat actgcaccta ttatagtaga accagaccta gatgtacctg
     2881 gtataagagc tagacactgg aataatccta tacaaagagc taatttatat gtaacttgag
     2941 aaaaatcttt atacttaggt tttttatttc tcttttcaag ccctatcatt attataccat
     3001 atacaataag agcaatagca actactgttg aattaaagaa taatctatct atatcatctt
     3061 caaacagtat tccaataact ccagatggta taactgcaat tataacctta agccataaac
     3121 ggactgtttc ttttttctgc ttaatattct ttgcgctatc aaaaggattt aattttctaa
     3181 aaaatataac aagtacagct aatattgaac caaattgaat tacaactaaa aatgtactta
     3241 taaatgtatt tgaaaaattc aattttataa actcatcaac taatatcata tgccctgtac
     3301 tacttactgg tagccattct gttattccct gaactatgcc caaaatgact gccttaaaaa
     3361 tttcaagcaa actaaatgtt tccaaatcaa aatacctcca atacaaattt atagtagcat
     3421 attttattca tagattaata aaaaaatgcc accttttact aacttataat accatagtac
     3481 tgtgtagtgc acaatggaag attgatattt tcataaaacc ttcataatat aacaaaataa
     3541 acttaaaagt tatctattga ataaggggta tatttgaggg ggaaatatat ggtatagtaa
     3601 aagtatagct attttatttt atgaaaagga aaggagttat tatgtatagt acaaaaatac
     3661 tagatgatta tgagtatgaa acttttaatg ttttttctac aaaattaaat ccaaacacga
     3721 aacacgatta tttatcaaag gttatactgt ttaaagaatt tttaaaaggg aaagaattga
     3781 tttatgctac gaaagaagat tgtaaaaatt ttgtagacta tatacagact aaatatgcga
     3841 aatcaacttg tgaaaagata tatagttatt tacatagttt ttataacttc ttaaaaaaag
     3901 aaggatacat agatataaat ccatttagat atgtagaaaa acccacagta acaagaataa
     3961 aaacaaaaga tgatgttcta agtatacaag aaataaataa acttataggt atcctaccaa
     4021 aattaaatat aagagataga gtaataatag tctgtctagt tactactggg tgtttattaa
     4081 atgaattagt tagcttaaag tggaaagacc ttatggtaga tgaaaacgat aactcatatg
     4141 taaggttagg taagggaaga aaagaaagag tagttaaatt acatccatac ttttttaaac
     4201 ttttagaaga ttacaggaat tattctgggc ttccagaggt aataatacct agtgatgatt
     4261 ttatttttac aactcaaaag agtaattcta taacagatag aaatgtcaga cttatagtca
     4321 aaaaagcatt agatttagct ggactttctc aatattcagc aagagatttt agacattcat
     4381 ttgcagcaat aagtttaagg cttggggcag atgaaagtga tgtaaaaaat cagcttggat
     4441 ggagtgataa atactatgca attagatata agtatgtatt aaattttgtt gatagtgaaa
     4501 tagtggatta cttaattgaa aaagataatt tggatataaa taaaaaatag taataaataa
     4561 aaaatactat atataaatgt gaacaaaaaa taaaaaagtc ttgttatagt tcgtaaaatt
     4621 atatataatt atttatgtac taatgtgtac acagtaatca cacgacaaaa gatttaagga
     4681 aggtgctaat ataatgatag ataataaata tactacttac tcaaacccac ttacagacag
     4741 atattgtagc aaggacatga gttatatatt ttctccacaa tttaaattct ctacatggag
     4801 aaggttatgg gttgcacttg cagaaggtga acatgagtta ggaatcaata taactgaaga
     4861 acaattagat gagctaagag ctaatataga taatataaat tttgatgaag ctagaaaaat
     4921 agaaaaagaa gtaagacatg atgtaatgtc tcacgtaaaa gcttatggtt tacaatgtga
     4981 aaaagccaaa ggaataatac atttaggagc aacatctgca tatgttggag ataatacaga
     5041 tgttatacaa atgaatgaag ctttaaaact tctaagagta aagcttgtaa atttaataaa
     5101 caatttaaaa gaatttgcac ttaagtacaa agatatgcca gctcttggat ttacacactt
     5161 ccaagctgct caacttacaa cagtaggaaa aagagcttgt ttatgggctc aagatttaat
     5221 tcttgatttg gaagatttaa actatagaat agacaacatg aagttaagag gagttaaagg
     5281 tactactggt actcaagcaa gttttttaag cttatttgaa ggagaccacg aaaaagtaaa
     5341 agagttagat aagaaaattt gtaaaaagat gggataccat tcttcctatg cagtaagtgg
     5401 acagacatat actagaaagt tagattatca agtaataagc atactatcag ggatagctca
     5461 aagtatgcat aagatgacta atgatataag acttttacaa agtttaaaag aattagaaga
     5521 gccatttgaa aaaaatcaaa taggttcatc agcaatggct tataagagga atccaatgag
     5581 aagtgagaga atagcatctt tatctaagta tataatctct gaatctataa gccctgcaat
     5641 ggtacaagcc actcaatggt tagagagaac attagatgac tcagccaata agagattggc
     5701 aatacctcaa gcatttatgg cagcagatgc tatacttgaa atagggataa atgtaactga
     5761 tggtttagta gtatatgaaa atatgataaa tagacgtgta aatgaagaac ttccttttat
     5821 ggctacagaa acaatactta tggaagctgt aaaaagaggt ggagatagac aggaattgca
     5881 tgaaataata agagaatatt ctatgaaagc tgcctataga gtaaagcatg aaggcaagga
     5941 taataactta attgaactta taatgaatga tgattcattt aaaatgagca aggaagaaat
     6001 actatctata atggacccaa aaaactttat aggtagagct ccagaacaag ttgtagaatt
     6061 tgtaaatgaa gttgtagaac ctgctataaa agattataag gaagacttag gaaaagtaga
     6121 tgtagaccta agagtataga attgtacaga taaaatataa ttaaaaacgg ctatcttatt
     6181 tataaataag atagccattt taatgggcta gcgtcttctc tccaccgctg tgaagagttc
     6241 ttctttactt actataccat gaatttaata aaatagctat ataaataata tttaatagga
     6301 ataatataaa taaaaataaa aaaatgataa gattactaat taaatttaaa cattcagcaa
     6361 aaactattgt acttgcaata tttttaatgt aaactgaaat aaagttattt agataattta
     6421 ataaatttta aaacaaccga ggtgattata tgaactatat agtaaaagat accataagtt
     6481 ttttagtaaa aacatttcct aagatttatt ctagcctgta cttagaagac ctaaaaaaat
     6541 ttgcaccaga ttataatgta aataaaacac aattaagagc attagttttc ataaaaaatt
     6601 atggagtaat aagtatgact gatttatgct ctaagttaaa tatagaaaaa ggcagtttaa
     6661 ctagtatggt tgatgattta actgataaga agtatgtaac aaggaaaaga gatttagttg
     6721 atagaagaaa atatctaatt gacattactg aagagggaga taagatagca actgatttta
     6781 tggataaatt aagtgatggt ttaggagaaa aactttctaa attaactgaa gaagacagaa
     6841 aaaaatattt agaagcaata aatactcttc aatatatatt aaacaaagaa gaatttagat
     6901 agaaaaaata taaaaatttt tgtatttaat ggtactaaga ttaattcttt gttattcatt
     6961 taactatata attataagtc taaatacaat ctaaaaagta catcttaaat tttcttgatg
     7021 tactttttta taatttctct aactaaggca agacaagttt tagatgcttt aattaaagtg
     7081 aataaagatt actggccaac actatataaa gaaaatgaaa aatagtcagg tctaagtctt
     7141 aattaatata ttaattaaga aatgtcccct tttcaaataa tatctgaaaa ggggacattt
     7201 tatatcatta aatttatata aattaagttt aaaaaattat ttttgtaaag ctacatattg
     7261 ctctaaacct tcttttaata atgtagcagc tctatgaagg tctttttcat ttaagatgta
     7321 agctaatctt atttcatcac gtcctaaacc tggagtagca tagaatcctt cagcagggca
     7381 aaccataact gtttcattat ctttattaaa ttcttttaac atccaaattg tgaaatcttc
     7441 tgcattttca actggtaatt tagcaactat atagaaagca ccagttggtt ttttacatat
     7501 aacaccttca accttcatta attcattgta taatacatct cttctttttt tgtactcttc
     7561 attaacttct ttgaagtaag aaacaggagt tttgtataac tcaacagctc ctatttgctc
     7621 taatgtagga acacaaagtc ttccttgaca taatttaagt atttgagcga taagaccttt
     7681 atttttgcaa gcgatagaac caattctagc accacaagca ctgtatctct tagaaacact
     7741 gtctattatt ataactctat cctgtacacc ctctaagtta ccacagcttg tatactcaag
     7801 cccatcataa acaaattctc tataaacttc gtctgctatt atccataaat ctttttcttt
     7861 agctatttca gctaggatat taagttcttc ttttgtataa attgcaccag ttggatttcc
     7921 tgggttagaa agaattatag ccttagtttt atcatctact ttagacaata tttcttcttt
     7981 agatggaagg tggaaaccgt tttctgcctt tgttgtaaca gcattaactt ctacgtttac
     8041 agattgacca aatccattgt agtttgtata gaatggttct ggaactaata gattatctcc
     8101 tgggtcacaa actgccatca tagtgaaaag taaagcttca ctaccaccgt tagttactaa
     8161 taattcatct ttttcaaaat tcatatcgta agttttatag taattttgaa gtgcttctaa
     8221 taattctgga ataccttctg atgtagcata ttctaagact tcgctatcaa agtttttaac
     8281 agcatcaaaa aatcctttag gagtttttat gtctggttgt cctatgttta agtgatatac
     8341 tttgattcct ttatcttttg ctgcttgtgc aaatggaaca agttttctga ttggagaggc
     8401 ttgcattgca ctaactctgt ttgaataatt cattttaata ccccctattt aatttaaata
     8461 cgtatattgt attatgtaaa ttgtatgttt gaatacaaga catctttaca agataataat
     8521 aacatactta tgggtaaaaa tcatcaaaaa aaattaatat agctttaaaa taaactttta
     8581 acaaattata gtaaaatacc catgtttatg ccactaaatt atgtattcat aaattaattt
     8641 atag
//
LOCUS       DAMJZE010000162         8435 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJZE010000162 DAMJZE010000000
VERSION     DAMJZE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.166"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     regulatory      57..142
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            266..6229
                     /locus_tag="PC541_003104"
     CDS             266..6229
                     /locus_tag="PC541_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2840545.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            6439..8319
                     /locus_tag="PC541_003105"
     CDS             6439..8319
                     /locus_tag="PC541_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2840546.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
BASE COUNT         3598 a          968 c         1573 g         2296 t
ORIGIN      
        1 taaaaaaatt gtattaaaaa gtttaaatat agtataaaat gggtaagaaa gtagtgaaaa
       61 ttaaatggca aaattaggga aatctaatga cgcaaaacta tagggattaa agcttttaaa
      121 gccatatcag ccagttgcta aaaagagtaa agtcttttta ttttataatt aagattctct
      181 ttgagtgtct ttttttattg tctgaaactt atagtattta aattcatgaa aaattagata
      241 tttattaagg gaggattgtt atttcatgaa aaaggcaata tcttgtgtac tagcagtatc
      301 tatgtgtagt gcaccactaa acgtttttgc agaacctata ttagagggga agctaagggc
      361 agttgaaaaa tcagttacag aaaagttaag ggggagtctt gaagtagatt taaatttttc
      421 actgccaata aaaaatagcg attcagaaag tatgactaat atgcagttac gattaaaaga
      481 tgatagtaac aatagtggaa taataaagct tggagaaaaa gattcaggaa cagtaaatgt
      541 tgggacagat aatatagaat atactataaa aaaattatct gcaagtagaa gtgaaataaa
      601 aaataaagat gagaatatat catattataa tattgtattt aataatttac ctgttggaaa
      661 atacaatgta gaagtctctg gagatggatt taagaacaaa gaaattaaag acattgatat
      721 atcaagttat tcacaaagag tcttattaag caatagagca agtgtagata ataaaaataa
      781 acctttaaat tatgaattgt tcttaatggg tgatgtaaat ggagacggta aagtagataa
      841 gagtgattat aataaggttt tagagaatat agattcaaat aaaagagaat ttgatttaaa
      901 tagagatgga aaggtagata ttgttgactt agattatgta cagaaaaatt taggacaaag
      961 tgaagatgca aaaagcctag aaagtatagt atctaccaat cctatagtag atacaagtaa
     1021 ggttgaatta aaaggaaata gtgatgtaga tattaatggt aatgtagaaa atttatttag
     1081 tggagaaaat aatggagtta caatatcttc aaaaaatgat gaaatatctg aggaaaaacc
     1141 agcagtaatg gatatagctt tatcagaagc acttaatatg gaagtcatca atataaaagc
     1201 tccagaaggt actgctccag caaaaggaat agttgaggtt actgacaaag atggtaatgt
     1261 tcaacagatt ccttatgaaa acaaagaata tatgaaaaac ttaaaagatg aggagcaaca
     1321 agaggaagta aataacgaac aagaagaagt aaatcaagaa gaagtaaata ataaagatga
     1381 aaatataaac cagactgata ataactcaaa taataaagat gaaaataatg atgaacaaca
     1441 ggaaaatagc gataaacaag aagtaaactc taataccaat caagataaaa ataatgataa
     1501 agaaaattca tcaatggata aacctaattc agatgaagat actccaaaag aagagggtaa
     1561 tatcagtaaa gatgataact ctacaacatc tacatatagt aagtcaacat tagaaacata
     1621 tgcagatgtt caaaatagtg atatagtaat aaatcttggt aaacaagtag cagtaaaaaa
     1681 gataactata aaaataacag ctacaacagc aaacgataga aatcttgcag aaatatcaaa
     1741 agttgaattt ttaaacaatg tatataaaga aataccaaag ccagagatga atatacctaa
     1801 aataaataaa atatcgacta gcactgctgt tggaaatgaa aaagtatctt tttattggaa
     1861 taatgaagta aatgtatcag catacgaact tatactagat aaaattgata gtaagggaaa
     1921 agtattatct actaaaaaat tacaaaccag taaaaataca atagaagtta gagatataga
     1981 agcatatgga ctatataggg ttagcataca atctttaaat ggagattggt ctagtggtta
     2041 taaagatgca acaccagaaa acttagacgg agtaccagaa aatgtagatt caaactataa
     2101 acctatacaa tttagcccag atagtatatt agaatttcaa gttgttccag acacaaaacc
     2161 agaaccacca gaaggtataa atgttaaagg taaatttaaa tctcttgatg taagctggaa
     2221 aaatcataaa caagctaaag attttgactt gtactataaa gaaaaaggct ctaatgggtc
     2281 ttggataaaa tacaatgata agcttattcg tgggacaagt tcaaagatag aaggtcttaa
     2341 ggataataca acatatgaag ttagaatgac agctactaac cacttgggaa ctagtggaat
     2401 gtctaaaaca tatataggta caactgaaag catggatgca ccaacaacac ctaactatag
     2461 attaattaat actcctaaag agaatggtgg atttggaata ccaacaaatc acattgtaga
     2521 tgttacttat ccagcaggat atgacaaaag tgaatatagt gataaaaatc catttgataa
     2581 attcaatgta gtagatgggg actttacaac acattggaca ttcccaacat ataatgcagc
     2641 tgagggaacg aatagaggac caataataac ttttgataaa tcttatacta tggatacttt
     2701 tatggctaca cctcgttttg atgtttctga taagacaata aatgactttg aggtacgata
     2761 ttgggatgaa aatggtcaat tacacaatct gggggatgtt ccttttgaaa caaagacaga
     2821 ccctgtaaat aataataagt atgtaatggt tagattaaaa gaaccaataa ctgctaccaa
     2881 gatacaagta agtgtttgtt catcctattc atataagtct aatatatcag aacttaagtt
     2941 ttatgagtat gacagtatag aagatgatgt aagaaatcta tttatggatg acctacaagt
     3001 tgaattaaaa gaagatgtaa ctcaagaaaa aataactgaa ttaaaagata gattaaatac
     3061 tccagataaa gctagtggag aatatcatcc atataagaca gttattgaga gagaattaaa
     3121 attagctcaa gacttatata atgatagagc cacacttact gatgtaacta ctgtaaatca
     3181 agaaataaat aaattaggaa aatataaaga taaaaacgga cagtatactg taaattccaa
     3241 taatttaggt atgcaaaatg actggcaggc attaggagta gctgctagag caggagatga
     3301 aatcacagta tatgttggaa gtaaaagtgg aaaaactcct aagttgatat atacacagta
     3361 ctatggagaa tctggtgcat ataagagtgg agaaataaat cttaacactg gaaaaaatgt
     3421 aattcaatta agcaaacttc atagtttaga tatagaatgt ggaggaagtt tatacataag
     3481 atacgctgaa gatactccaa gtggaggaga tataaaagta cgtgtagcag gagctactaa
     3541 gataccacac cttaatctaa atggaacaat aaatgataag agtcctgcgg gagtttctga
     3601 atctaagaaa aagataaaag catatataga agaattagct agatataaag atgctgtaaa
     3661 aggagaacca tattatccag gaacaaatgt tggtaattca tatggatatt cagaaaaaac
     3721 tggtgtgtta aacactacag atattgaaag tgataaagta acattaaatg ttcctgcaac
     3781 agctgtatat caagggatat caaaaggaaa tgctgattta aatacacaag tagatagatt
     3841 atataatagt atgcttgcat gggaacaaat aatggattta gtttactcag aaagaggagt
     3901 atttaaaaca caagatttag atggtaatgg aactgtagat gataatgaat ttaacttaac
     3961 taaaaatgat atggctccaa aatctcgtat gaacataaaa taccaaagaa tgtttattgg
     4021 agcatttatg tatgcttctg gattacatgt aggagtagaa tatggttcag ttccagggct
     4081 tatgaatgga gtacctttcc aaatagatga tagtggaaaa gctacaggag gaaacttatt
     4141 tggatgggga ataggtcatg agataggaca tgttacagat attggaaaga tgacatatag
     4201 cgaaactagt aacaatgtac ttgctttact tgctcagacc tttgatgata agacacattc
     4261 aagaattgaa ggttcaacaa tggataagat atatgagcat gtaacttcaa atagcttagg
     4321 tataccaagc aatgtatttg aaaggcttgg aatgttatgg caattacatc tagcttatga
     4381 tgatgatttt acgggttcaa tgttaaagaa taactcagat gctgatttaa gtaatgatac
     4441 attctatgca aagatttcaa gaaaatacag agcactttca agtagtgacc caattaatag
     4501 tttgccaaaa gaccaaatgc tagtagcaat ggcatcaagt gttgttgaaa aagatttaag
     4561 agattacttt aaggcatggg gagtagaaat tacaccagaa ttaaacagta taatggatag
     4621 taagaactac gaaaaagaat caagaaagat acaatatgta aatgacgaag ctagaagaaa
     4681 gattttaaat gaaaatatag ctagtatggc aaaagatact aaagtaagtg ctagtttttc
     4741 tgatggaata caaagtggtt ctctagttaa atctaataaa atatctatag acctagatgt
     4801 agataaggat aaagataaga tactaggata cgaaataata agaagtgatg gaaattattt
     4861 agatggtgaa aatggaacac aggtaaaata cagaacagtt ggatttgtgg atgcaaaagg
     4921 aaatagtaag actaaattta ctgataatat atctcctctt aataacagag catttacata
     4981 taaggtagtt gcatatgatt atcacctaaa tccaacagaa gaatttgaag ttggtactgt
     5041 aaaattatca gatgaaggga aaataaataa atcagcatgg tcattcataa caaatactgt
     5101 ttctgacggt gatgttagaa ctgaaaatga ctcacatgga ccattacaaa atcctgaaat
     5161 tgataatata aaagataatg atgcaaatac tacatataaa ggaaagataa taagtaaagc
     5221 agagtggaac aaagaccctc aaaaagaccc tgatataaat gtagaggaaa atccatatat
     5281 aatagtagac atgaaagaaa cattacctat agtaggacta aaatatacaa aaccagagac
     5341 agaagctagg aagttctcat taaaaggatt atttaacttt agaaagaata ctagcacaac
     5401 atacaatcct ttaactaatt acaaaataca agtaagtaat gataaaaaga actgggaaga
     5461 tgtaagtcaa ggaacttttg aatatggaca agatttagtt ggtggaggaa aagaccaaga
     5521 taatgaagct agagttacat ttaataaaga tggtaaactt tggacatacc aagctagata
     5581 tgtaaaacta atctcaaata ataagtctaa tatagaagtt gcagaattag atataatagg
     5641 acctccaggt gataatatag aaattggcac aatgaataat gcagacgaaa ctcgtactaa
     5701 tggaattgga aaattagcag aagattatgt atatcaaagt gatgactcta gcacaagtga
     5761 agatgaaaca ggtaagattc cagcgggttc aatagttatt acaggtgaat acagtggaaa
     5821 tccagcattt aatttaccac tacttataga taaaaacaat aagactatat ctggagaagc
     5881 acttttattt gcagaagttc cagaaaaagg tgaactaggt gaaatcagta aaggtacatg
     5941 ggtttactat atgccaaaag aaaactttga ttctttaagt gataaggtta aggctgaact
     6001 atatagatac aatgaccttc aaggagatac tccagtaggt caaagatttg taagtgatac
     6061 aatgtatgta cctgtaggag ctaagaatta tgatgagtta aagacaataa gtcttactga
     6121 tagtaatagt aaagctagaa aagctacaag aaattctatt gatatcagta ataagaaggt
     6181 tataactgta agcaataaag taaagggtaa tattgttaga gataaatagt aggtggaact
     6241 ctaaaaacgt aaaaatacta acactaacat tgtatatcta ataagattag gtactagaat
     6301 agttttaaaa tagtagatat taaaagtaaa gtatataaat gtaatcataa atttagggta
     6361 atttataaat taaatataaa agctaggtgg ataagtaaat ttatccactt agttaatgat
     6421 agataaggac gggtgttaat gagtaaaaga aaatctttta taagaactat agcagtatca
     6481 actatggcag tagctgtaac tggtagtgct acatgtgcat atgcagctcc agttttacaa
     6541 ggaacaaaaa cgtatgagaa agtaaatact atagatatat ctgtggactc agtagaaaac
     6601 atagtatatt catttcaagc cagtataaaa gtacaaggtg aggttgaagt agtagataat
     6661 gagcagaaag aaaagattac ttggtctgat aatataaaat ctcagataaa aagtggaaat
     6721 gcaaatgcta cttgtagagc agaatacaac aaatcaagta atacaactac attagatata
     6781 tatgttacat caaatgaaga cttactagac ggaaatagat tgaatatagg aagaatatct
     6841 gtaaaaaaat ctggaagtaa ctcaaatgct gactataaag ttttaggaaa aggaacaaat
     6901 gataaaccag ctttaaaaat tgtaacatat aacaacaaaa cagtggatta tgaaaatata
     6961 agctctgatg aaggattaat atttaccctt attaatgaga gtgaagtaaa accgattggg
     7021 ggaactggtt ctagtaaaaa tgaccctgaa aagtataagg tagagaaaag tgaagcactt
     7081 gaatatttat taaacaatat taggataaac tattctattg ttagtaaaga aacacaagaa
     7141 agtggttcaa atgtaatatt aaaacttgga ttagcacaaa aaacaactaa gggaagaaaa
     7201 gttacaataa acaaatatgt tgaagttaca cttccaaaaa gtttagaata catagttgaa
     7261 aatgaattat caaaaccgga tgaattacca ccagataatg gtagtggtgg aaataatggt
     7321 ggaggaagta attcaggcgg ctcatctagt ggtggaagct ctggaggagg aaattcaagt
     7381 gattctacat ctaatgtcac agtcaaaaaa ttaaaaggtg atgatagatt tgagacagct
     7441 atcaaaatat ctcaaagtgg ttggacaaaa tcagatacag ttgtaatagt aaatggagaa
     7501 gataaaagta tggtggatgg gcttacagct acaccacttg ctagtgtaaa aaactcaccg
     7561 attcttttat catcaaatga taaacttcct caaaaaactg tagaagaatt gaaaaggcta
     7621 aatccatcaa aagtagttgt gataggtgga aacaactcaa tgccaaattc agttgttgaa
     7681 gctatcaaag ctgtaaattc aaaaatatca gtacaaagaa taggtggaga tacaagatat
     7741 caaacttcta ttaatatagc aaaagaaata gataagacga ataatgtaag caagttgtat
     7801 ataggagcag gaaatggaga agctgattca ttatcaatag catcactggc tggtaaagaa
     7861 aaaactccaa tagtacttac acaaaaagat ggtgtagata atgaagctga acaatttatt
     7921 aaatcaaata aggtgtcaaa tatatatttt attggtggag tagaaaaaat atctaacaag
     7981 gctattgaac aagttggaaa aatagcaaac aaagatatat caaacaatag ggtggcaggt
     8041 caaactagac aagagacgaa tgccaaagta atagataaat tctactctca atctaagcta
     8101 gatggagttg tagttgcaaa tcaagataaa cttatagatg ctttagcagt tggaccatta
     8161 gcagctaaaa acaattcacc agttatactt gctacaaata cgttagataa atcacaagaa
     8221 tcgtcattaa aaggtaaaaa ttcatcaaag ttgtttgaag taggtggagg aatagcctca
     8281 tctgtaatag acaagattaa gagtttaatt gaaaaataaa tattaagtaa atagaaaata
     8341 agaatgtatt tagatataaa gttatatatt taaatgcatt cttattttat tttcattatc
     8401 ttaacaatct aataatattg aggttaaaat gtata
//
LOCUS       DAMJZE010000163         8376 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJZE010000163 DAMJZE010000000
VERSION     DAMJZE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.167"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            58..213
                     /locus_tag="PC541_003106"
     CDS             58..213
                     /locus_tag="PC541_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840547.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            343..1356
                     /gene="aroF"
                     /locus_tag="PC541_003107"
     CDS             343..1356
                     /gene="aroF"
                     /locus_tag="PC541_003107"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2840548.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1378..2442
                     /locus_tag="PC541_003108"
     CDS             1378..2442
                     /locus_tag="PC541_003108"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2840549.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2442..3755
                     /gene="aroA"
                     /locus_tag="PC541_003109"
     CDS             2442..3755
                     /gene="aroA"
                     /locus_tag="PC541_003109"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2840550.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3733..4812
                     /gene="aroC"
                     /locus_tag="PC541_003110"
     CDS             3733..4812
                     /gene="aroC"
                     /locus_tag="PC541_003110"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2840551.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4815..6011
                     /gene="pheA"
                     /locus_tag="PC541_003111"
     CDS             4815..6011
                     /gene="pheA"
                     /locus_tag="PC541_003111"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2840552.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKTAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6058..6867
                     /gene="aroE"
                     /locus_tag="PC541_003112"
     CDS             6058..6867
                     /gene="aroE"
                     /locus_tag="PC541_003112"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2840553.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6879..7412
                     /locus_tag="PC541_003113"
     CDS             6879..7412
                     /locus_tag="PC541_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2840554.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7433..8272
                     /locus_tag="PC541_003114"
     CDS             7433..8272
                     /locus_tag="PC541_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2840555.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3442 a          828 c         1511 g         2595 t
ORIGIN      
        1 tttgttataa ttattaaaca agaagaataa taaattataa aaatggagga taatactatg
       61 aaaggaatat taaaagaact aacagcaaaa ctttatagct ttttttactt tatgttctgg
      121 ggcttttatt ttagcgctgg ctatttcttt tacaaaaata tatgtacttc ctttctggtg
      181 aggccttatt ttagtgattt tgagcataag taaattttat aaaaatgatt attttatata
      241 tttatataag atttagtgct tgatagatta ttttagagaa ctcattagtg agttctcttt
      301 tttattttaa atataaaatt ataatattgg gaggtagtaa aaatgatagt agtattaaaa
      361 atgggagcag ataaaaatga agttaaaaaa ttaattgagg ctattgggag agaaggagta
      421 gaagttaatc ccatagatgg aacagaatta actgttttag gtttagttgg agatactagt
      481 aaaatagatg ccaaaagaat agaagcaaat aaggtagttg aaaaagttat gcatgtggta
      541 gagcctttta aaaaggccaa tagaaaattt catccagaac cttcaatcat aaatgttaat
      601 ggaatggaaa ttggttcaaa gaaaattgct atgatagctg ggccttgttc tgttgaaaca
      661 gaagaccaaa tagtatctat agctaaagat gtgaaaaaat caggtgcagg atttttgaga
      721 ggtggagcat tcaaaccaag aacatcacca tacgctttcc aaggattgaa atatgatgga
      781 ttggatttat taaaaaaagc aaaagaaaaa acagggcttc ctatagttac agaaataatg
      841 tcaacacaag atatagatat atttgaagaa aatgtagatg taattcaagt tggagctaga
      901 aacatgcaaa actttgattt attaaaagaa ttaggtaaaa caaataaaac aatactttta
      961 aaaagaggat tatctgctac aattgaagag tggctaatgt cagcagagta tataatggct
     1021 ggaggaaatg aaaatgtagt tctttgtgaa agaggtataa gaacttttga aacatatact
     1081 agaaatactt tggatttaag tgcaatttta gctgtgaaga aacttagtca tttaccagtt
     1141 atagttgacc caagtcatgc agcaggtaaa tcatggatgg ttgactcttt atctaaggca
     1201 gcaattgcag ttggagcaga tggtctaata atagaagttc ataatgaccc agcacatgca
     1261 ctttgtgatg gaaaacaatc gattaagcca aatgaatatg atgaattgat aagtgaatta
     1321 aagactatcg catcagcagt tggtagagaa atataatcta taaaaggatg gttaaacgtg
     1381 gaagaaaaac tattagtaaa tttaaaagaa aatagctaca atatatttat taaaaaaaat
     1441 atactaaaga atataggaga agaaataaaa aaaatatatt ttggaaataa gatttttatt
     1501 gtaactgatg aaaatatagc aaaattatac ttggataaag taaaaaataa tttaagtgaa
     1561 tctggatttg aaattaaatc tttaatatta aaatctggtg aagaaacaaa atcatttaat
     1621 actcttccta aaatatacga tagtttactt gactttaagt taactagaaa agatttgatt
     1681 attactttag gaggaggggt aataggtgat ttaggtggat ttgcagcagc aactttttta
     1741 agaggtataa gttttattca aattccaaca tctttattag cacaagttga tagttctgta
     1801 ggtggaaaag ttggagttga ccttgagaga ggcaaaaact tagtgggaag cttttaccaa
     1861 ccaaaggctg tgtttatcga cccaaatgta ttattatcac ttccagaaaa attctataga
     1921 gatggaatgg cagaagttat aaaatatgga tgtataaaag acaaaacttt tttcaataaa
     1981 cttttaagtt taaaaaatag agaagaagta atgcaacata tagaatacat tatacataaa
     2041 tgttgcttta ttaaaaaaac tgtagttgaa gctgatgaaa aagactttgg agatagaatg
     2101 cttctaaact ttggccatac attaggtcat gctattgaga aatactacaa ttttacaggt
     2161 tatacccatg gagaagctgt tgccatagga atgtataaga taactttaga aagtgaaaag
     2221 ataggaatta caaaaaaagg tacatcagaa cttataaaat caattttaat gaattataat
     2281 ttgccttatg agattgaatt aagagataat aaatctatag tagatgctat atctcttgat
     2341 aaaaaaaatc taggtgatac attaaaaata atacttttaa aagatattgg agagagtatt
     2401 atatataata ctacttctaa attttttcag gaggtaagat aatgggaagt ctaaaaatat
     2461 atccaagtaa attatctgga gatgtaaaaa taccaccatc aaaaagtatg gcacataggg
     2521 cagttatttg ttcatctcta agcaatggaa aaagtagaat ttctaatata gatttttctg
     2581 atgatataat agctactata agagcaatga catcattagg agctattata gaaaagaaag
     2641 aagatatttt agaaatttcg ggaatttttt ctaaagaagg tattttaaat agagagaatc
     2701 agttgaatca acctaaattg acaatagatt gtaatgaatc aggctctaca ttgagatttt
     2761 tagtacctat atctttaaca tttgatggtg taaaaaggtt tataggtaga ggtaatcttg
     2821 gaaaaagacc acttgatacc tattatgaga tatttgacag acagaatata aaatatagtt
     2881 acaaagaaaa tcagttagat ttgattatat caggtaagct taaaccagat gaatttagag
     2941 taaaaggaaa tataagttct cagtttatca caggtttact gtttatattg cctacgctag
     3001 aaagtgattc aaagataata attactactg agctagaatc taaaggatat ttagatttaa
     3061 ctctttcaac gataaaagat tttggggtag aaattataaa caataactat aaagaattta
     3121 ttataaaagg aaatcaaacg tataaagcga gagattataa agtagaagga gattattctc
     3181 aaggagcatt ttatttatca gcagatgcta taggagaaga tattagcata ttagacctta
     3241 aagaagattc tcttcaaggg gatagtgaag tagttgaaat tttatctagg atgggaatgg
     3301 aaatattaag agaaggcaac aaaataaaag gaataacaaa tggtttaaat ggtactttga
     3361 tagatgcatc tcaatgccca gatataatac ctgtattatc tgtagtagca tccttaagta
     3421 tagggaggac tacaataatt aatgctggta ggcttagaat taaagaatgt gatagacttc
     3481 atgcaataaa tgttgagtta agtaaacttg gagcaaatat agaggagaaa gaggatagtt
     3541 tgataatcga aggagtaagc aagttaaatg gtggagtaga ggtgtggagt cacaaagacc
     3601 atagaattgc tatgacactt gcaattgctt cttgtagatg tgataaacca ataatattaa
     3661 aagattttga gtgtgtatca aaatcttatc cacatttttt taaagatttt aagatgttag
     3721 gaggtcgaat tgatgagtgg aatatgggga aataatttaa aagtatcaat ttttggagaa
     3781 tctcatggaa atgctatagg tataaatata gatggtcttc cttcggggat tgaacttgac
     3841 cttgataata ttgataaaga aatgaagaga agagcacctg gaaaaaattc tatatctact
     3901 tcaagaaatg aaagtgatat accagaaatt ctaagtggat attttaatgg aagaactaca
     3961 ggaacgccat tatgtgcaat cataagaaat agtgatactc gttcaaaaga ttatggagag
     4021 cttaaaaatt taatgagacc aggacatgca gatttcacag gtaatgtgag atattctgga
     4081 tttaatgact atagaggggg aggtcatttt tcaggtagga taacagctcc attagtattt
     4141 tgtggggcta tatgtaaaca aattttatca caaaaaggaa tagagatagg agcacacata
     4201 aaaaaaatta aaaatataga agatatgagt tttgattatg taaatatttc aaaacaacag
     4261 ttatcaaatt tgcaaacttt ggaactacct ttacttgatt tatcaaagga agaagccatg
     4321 aaaaatacga ttatagatgc aaaaaatcaa ggagattcag ttggtggtat aattgagtgt
     4381 gcagtagtag gtattaatgt agggcttgga aatccatttt ttgactctgt tgaatctact
     4441 ttatcacatt tattgttttc tgttcctgct gtaaaaggtg tagagtttgg tttaggtttt
     4501 gaacttgcag atatgtatgg gtctcaaagt aatgatgaaa tgtattatga aggaaatcaa
     4561 gttaagagca aaacaaataa caatggagga attataggag ggattactac tggaatgcca
     4621 ataattttca aagtggcaat taagccaact ccatcaatta gcagacaaca aaacacagtt
     4681 aatataaaag ataaaaagga cgatatttta tatattaaag gtagacatga cccttgtata
     4741 gttcaaaggg ctatacctgt aatagaagca gtaacagcca ttggaatttt tgatttgatg
     4801 aaaggacgat aataatggag gatttaacga gatatagaaa tgagattgac aatatagata
     4861 aagagcttat tcaattattt gaaaagagaa tgaatacagt tcttgaaatt gccagacata
     4921 aaatgaaaaa taacactact atacttcaaa aagatagaga agaaaaagta ctaagtagag
     4981 ctgtagataa tttagataat aaagcatatt cacaagaaac ggtacagttt tttaattcta
     5041 taatggaaat tagtagagga ttacaaaaaa gattgataga taatgatggt gagcagaaaa
     5101 tcgagtttag tttagattca tcaaataaga aatttgattt gtcaaatgta aatagatata
     5161 agtctttaaa agatgaatta aataaaaaaa atatcctagt aggatttcca ggaaaatcag
     5221 gttcatttac agaagaagca ctaaataaat tcttcaacaa aaaaatatca aaaaaacaat
     5281 ttaaagaatt tgaagatgtt ttcatagcac ttaaaaataa agaaataaac tatggaataa
     5341 tccccataga aaattcatct acaggagcta tctctgaaac ttatgacctt ttgagaaagt
     5401 acggttttta tatagttgga gaagaatgta taaaaataga ccaaaattta atagggataa
     5461 aagacacaaa gcttgaagat ataaaagaaa tatattctca tccacaaggt ataggacaat
     5521 gtagtaaatt tttaaaacaa aatagtacat ggaaattgat accatttaat aacacagcaa
     5581 caagtgcaga acttgtaaga aaacttcaag ataaaacaaa aacagcaatt gcaagcaaaa
     5641 aagctgcaag tatttatgga ttagaaataa taagtccttg tataaatgat attgcaaaca
     5701 actatacaag atttgtagta atcagtaatc aaatacacat tgaagaagaa tcaaacaaaa
     5761 tgagtgtagt attttctgtt gaacatgaag caggaaaact ttataaagta cttggatact
     5821 ttgctaaaaa taatataaat atgacaaaaa tagaatcaag acctatgaaa aatgcctcat
     5881 ggagatattt cttttatata gattttgagt gttctatata caatgctaaa gtgtacgatt
     5941 tattagaatt gatagaacat aatacagcat attttaaatt tatgggagta tataaaaata
     6001 aaactgaata gataagataa atttttattt aatttataat tattaggagg tgtaagtatg
     6061 aattttttcg gtctagtagg tgaaaaactc tctcacagtg tttcacctca aatccacaag
     6121 agagtattcg agatattgaa tattgaaagt gcttataaaa actttgaaat ttcaaaagaa
     6181 gatatatcta agctagacgg tgctataaaa ttattaggaa tacaaggggt aaatgttact
     6241 gtaccatata aggagcgtat tatgaaatat cttgatttta tatcaccaga ggcaaaacgt
     6301 ataggagcag ttaatacaat tttacttaga gaaaatatgt tatatggtta caatacagat
     6361 tactttggtc ttgatagtat gtttaaaatg gctaatattg atgttcaagg aaaagtagca
     6421 gttatattag gaactggagg tgcttctaaa gctgctctta catattttat tgattctggt
     6481 atagaaaagc tttatgtttc aactagaaaa aaggatgata aaaaattatt aaatagtaaa
     6541 gctatattaa tagattatga agaacttaaa catataaaag gagatataat attaaatgct
     6601 acaccagtag gtatgtaccc taatgtagga atttcaccag tgtcaaaatc tattatacaa
     6661 aattttgata tattaataga tttaatttac aatcctggag aaacagagtt tttaagaatt
     6721 ggtaatagta tgggtaaaaa aacttgtgat ggattatata tgctggtggg gcaagcaata
     6781 aaatctcagg aaatttggca agatactaaa atagataata gtattttaga tgttatatat
     6841 aatgaactaa aattagaatt tttgtaaggg agattgttat gataaataaa acaaaagaaa
     6901 aattgatatt aataggtatg ccaggtagtg gaaaaactac aattggtaaa cttttggcca
     6961 aagaatataa ttgtagtttc tgtgatatgg atgattatat aattcaaata tctcaaaaaa
     7021 gtatagcaga gttgttttca gaaggagaag atatctttag gaactatgag actcaagcat
     7081 gtagagaact ttctataagt gataaaacag tgatatctac aggtggaggt gtaataaaaa
     7141 aggatgttaa tatggaaata ctaaaagaaa caggtataat aatttttatt gatagaccta
     7201 tacaaaagat tttggaagat ataaatatta attcaagacc attattaaaa aacggaaaag
     7261 atagattgta taatctttat aatgagagaa taaatcttta taaaaaattt tcagatatag
     7321 agattttaaa tgataaatca ttaaataatg ctgtatataa tattacaaat gcagtcagtg
     7381 aaaattttaa atttgacttt aaagaaaaat aatctagggg gaaataaaaa taatgaatat
     7441 tgttattgta ggactaggag ttataggagg ctcttttgct aaagcattaa aaaaagctgg
     7501 atatgaaaat gtatttggag tagatgtaga tttagaaact ttaaaaaaag cagaaaaagc
     7561 aaaaatcata aaaaaaggat gtactacagg taaagagtta tttaaaaaag ctgatttaat
     7621 tatattatct atatatccta gattagttgt agattttcta aataataata aaaacttttt
     7681 taaaaaaggg actataatta ctgatactac aggaataaaa gaaacactta tcaatgatgt
     7741 gttgcaaatt attccagatg atatagattt tatatttgga catccaatgg caggtaggga
     7801 aaaaaagggt attgattttg caagtgaaca agtttttaat ggcgcaaatt atataattac
     7861 tccaactggc agaaacaaca taaaaaattt agagttagtt gaaaatttaa ttttagagat
     7921 tggatttaaa agagtaaaaa agttaacttc acaaaaacat gatgagatta tagcttttac
     7981 ttctcagtta cctcatgtta tggcagttgc tcttataaat agtgatgaag aaggtagaga
     8041 tacgggtaaa tttattggag atagttatag agatttaact agaattgcaa atatgaatga
     8101 agacttatgg agtgaacttt ttttaggaaa tagagataat ttactaaaag ttatagaaaa
     8161 ctttgaatca gaagtaaatt tagtaaaaga agctatattt aacaatgata aaaataaatt
     8221 aatagagtat tttaaaaaat catcaataag aagagaagct ttagaaaaat aaatattaat
     8281 atttaagcta tatatagatg ggtataaata taagcgatag tgtatttaat tgtattaata
     8341 aatatggatt caatataatt aaatatgcta ttgata
//
LOCUS       DAMJZE010000164         8359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJZE010000164 DAMJZE010000000
VERSION     DAMJZE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.168"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..1606)
                     /locus_tag="PC541_003115"
     CDS             complement(<1..1606)
                     /locus_tag="PC541_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2840556.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVL"
     gene            complement(1596..2444)
                     /locus_tag="PC541_003116"
     CDS             complement(1596..2444)
                     /locus_tag="PC541_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2840557.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(2674..3726)
                     /locus_tag="PC541_003117"
     CDS             complement(2674..3726)
                     /locus_tag="PC541_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2840558.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(3726..4400)
                     /locus_tag="PC541_003118"
     CDS             complement(3726..4400)
                     /locus_tag="PC541_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840559.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            complement(4505..5341)
                     /locus_tag="PC541_003119"
     CDS             complement(4505..5341)
                     /locus_tag="PC541_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840560.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(5343..6386)
                     /locus_tag="PC541_003120"
     CDS             complement(5343..6386)
                     /locus_tag="PC541_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840561.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(6405..6944)
                     /locus_tag="PC541_003121"
     CDS             complement(6405..6944)
                     /locus_tag="PC541_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840562.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(7380..8069)
                     /locus_tag="PC541_003122"
     CDS             complement(7380..8069)
                     /locus_tag="PC541_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840563.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         2783 a         1453 c          810 g         3313 t
ORIGIN      
        1 ttaaaacact tatagatact atgaatatta aaactttact aatattggat atggatttaa
       61 atctagtatc acttttagtt agttcatcta agtccttatc atagttgact ataatttcat
      121 ctccattatt tcttatttgt atttcatcaa cttttaccca attattaaaa tctcttaaat
      181 tcataaattc tatattctgt ttttctaaat attctattgt gttttttaga tactctatgt
      241 ttaaatatga atgaaagaag aaccctccac taaaacctct tacaatggat aatttatcta
      301 aattttcttt tatattttga aaagtaaact tatcttctgg gtctatatat cctaaatttt
      361 cgggtataaa tatattaaac tcctctgtat ccctaataat ataaggatat gtatttgtgc
      421 taaatttttt atcattattt tgatgttgcc ctatataagt agaaaaatat ttttttaatt
      481 ctttatatcc ctctgattcc attgcataat gtggcgcttc aaatgcaaga ggataaattc
      541 cattctctat acatactcta agaccattca aaactctatc tttgacaaat atcttcatat
      601 tttcttttaa gggttcatct tttttaccat cccaaaattc atacccttct ccagaaactt
      661 cttctttctt gtattgatga gtatatccat gcaatatcac agttccacct ttatcttgca
      721 tatattttat tgctttgaca atttcagggc tttctgataa agttattacc ttatgatttt
      781 taggatttac atatgctggt attaatgcaa tcgtaaatgg tatattctta ctacttaaat
      841 aatccgctat ctctacaaga ttttttggtt ctctaaaagc atgtacatct tctattctta
      901 caaatatacg tcctttatca aacgtcttaa tattaaaaat atcattaagt gaatcgcaaa
      961 atatataaaa taaaactcca tctaaatcta attttgatac ataaaaaaga tttttatcat
     1021 ttattatata tggatactta tttaaggtat cattaatact tcctattact ttattacttg
     1081 tatcttttga ttctacaatg ttaaaaacat aatgttcatc caagttatat gatttacctc
     1141 tataatttac actaactata ttgtttgttt ttcctacata gtctaaattg tattttttat
     1201 gttccaaaag attttctata ccatatccta accaacatat agttccttta tataaattta
     1261 aggaactaat caagttttta gtgtttttat cattattata tgagttttca ttgattccta
     1321 aaataaatat atgactgtaa taatttttat ttatctcacc atcatagtta ctcaatttta
     1381 atagctctat atctgaacta aatcttccca atagagtcct cataatattt aaaatatctc
     1441 tattataagc tgtttctttc ttagaatcat atatgattaa taccttatct gtgctggaag
     1501 taatttctga tgcatatgaa gataagctaa aatttgatat tgtcagtact attgataata
     1561 caactactaa ttttaataga cttttattaa atattttaaa catcatattg aagttcctct
     1621 tcaactatat tcttaaaatt tatactatct ccaaaactac tcttatattg taaaaatgca
     1681 attttgacat ctatatttac atattttccc ctattgttta aatctaaatt taattctttt
     1741 atccttttct ttattctgtc tttaacaact tttgaacctt ccaaagatgt atttggcatt
     1801 agtatcccta tcatatcttt ttgtaaacta taaattatat cttcatttct tgtacattct
     1861 ataatatttg aaccaatata ttttactatt ttattagttt tgttttcacc aaatatagtc
     1921 tgtaggtttc cgtagtatgg taattttact atcataagag aaaaatcagt attatgtcta
     1981 atagatttac ttatctccat atttacatca ttataaaaaa tctttaaatt tctaagtcca
     2041 gtttcactat caatagtcac taattcctta tactgttcag atagtttttt atttgtattt
     2101 tgcaattcat ttatgctttt gttcagatta cccattataa ttgaagatac tggtgttgct
     2161 attatccaca tatatgatat aaattcaaca tccatattgt gagtaatatt gttatataaa
     2221 atataggatg tatacataaa tataattatt gaacttgtta tcaaccccac tactgaattt
     2281 gataaataag ttaacattat tataaaaaaa attattgcta acattatgaa cacattcaat
     2341 ttattttctg tcctaatcga tagtattact attccaacaa ttaaaaatat aaaaacgagc
     2401 aaagccaata ttaataaatc tattttttta tttatcctat tcatttttta taaatttaca
     2461 cctccaatta ttctattaag ttgcccattt tttctaatta ttattaaagt cattatacaa
     2521 ataagttttc atttattaat aaactattta aattgcatat gaatataact taaacaaaaa
     2581 ataaaactgt tcaataatta gaaaataaat tttctaaaaa agaacagttt tattaagtat
     2641 ttattaagta aatttactta aaccaacaag accctatttt gattttaact ctatccaagc
     2701 cttgtcatat aattttatat tttcaccaat atcttcaaaa gtttcacatt tatcaagcac
     2761 ttctttagat ggatatgcaa caggattctg agttatttct gggtctaata attttagtgc
     2821 tcctgtatta ggtgttgaat acccaatgta ctcagcattt acttttgcat tctctgggtc
     2881 taataaaaag tttataaaca gctcagcttc tttcttattt tgtgctgttg taggtacaca
     2941 catagcatct acccatttat tagttccctc tttaggtata gcataatcaa ggtcaggatt
     3001 ttgctccatt agtgtaactg catctccaga atacagcata cccattgcca cttctccacc
     3061 aagcaatcta tccttacctt catcatttac ataagccaat actaaatcct tttgtttcat
     3121 taaaagattt ttagcttcat ttatttcttt tggatttaca ctattcatgc tatatcctaa
     3181 ttttttaaga gttatcccta tagtatctct aacagagtca aacatcatta tatttccctt
     3241 atactttgga ttccaaagta tattccagct atccataggg tctttaactt ctttcttatt
     3301 gtacagtatt ccaagagttc cccacatata tgcaacacta tattcattag ttttgtcgta
     3361 tacaggattt aaaaaactct tatctaaata tttcatatta ggtatattct taaaatctaa
     3421 tttttccaaa agaccttcat ttttcatttt ctctaccata tagtctgaag gaaatactaa
     3481 atcatagtgt gtactaccac tctttacttt ttgatacatc atttcattag tatcataagt
     3541 tgaatattga acatctattc ctgtttcttt ttcaaactta tctataagag actcatctat
     3601 ataatctcca acattatata catttaaaac ttttgtagaa tcagttgctt tattacatcc
     3661 tgtaaataaa gtaactgtca tcatacctac agctataagg gatattgctt ttttaaactt
     3721 aatcattatt taatccccct tactatagat tcttttttat tagctagtaa aagtaaacct
     3781 agaaccactg caaacattat tgttgataat gcatttattt caggttttat acctcttcta
     3841 gccattccat agatttctat agaaagattg cttacaccat ttccagcatt aaagaaactt
     3901 attataaagt catctattga catagtaaat gcaattaaaa atccagaaac tatacctggt
     3961 tttatttgtg gtaaaattac ttttctaaga gcgtatatag gtgttgctcc caagtccata
     4021 gctgcatctt ctatattatc aggtaattgc ttcatttttg gcaaaactga aagtataacg
     4081 tatggcaaac aaaacattat atgtgccaat agcattgttg taaatccaaa ttccattttt
     4141 acaaatacaa acagactcat aagagctact gctgtaacta tctctgtatt taaaatagga
     4201 agatagttta catttagtat taatttctta ctcttacctc tcatcttatg tattcctata
     4261 gcacttatag ttcctactat tgtagatatt acagacgcta atatagctat tacaatagta
     4321 taatataaag cactcattat actctcattt tgaagtaatt gaccatacca cttccatgta
     4381 aatccattcc atcttgccat tgattttgaa tcattgaaag aaaatagtac caaagcaaat
     4441 atgggtgcat acaaaaataa gaatactaat gctaaatata tattagaagt tattttcttt
     4501 aattttaaaa tagtaaccca ccgccttctt tgtcagattc atctccaaat tttgacatta
     4561 tagacataga tattaatatt actatcatca taaatatcga tatagctgaa ccaaagttcc
     4621 aatctccaac aactgtaaac tgttgttcaa tcaaatctcc aaccagcatt attttacctc
     4681 cacctaacag tcttgatatt gcaaaagtag tgactgctgg cataaatacc atagttattc
     4741 cagacattac acctggcaaa ctcaaaggaa atataatttt tctaaaaact gtcatattat
     4801 ttgcacccaa gtcatgagca gcgtttatca aatcattatc catctttgat agtgctgtat
     4861 atattggaag aaccataaat ggtaaaaagt tatacaccat acctaaaagt atggcaaaat
     4921 tagtatacaa aattgctaat ggttgtattc caaaccaacc taaaaatgta tttaaaagtc
     4981 cattttttcc taatatagca acccatgcat atgttcttaa aagaaagttc atccacatag
     5041 gaagaataaa caataatatt aatgaacctc ttctacttac acgagccttt gaaattatat
     5101 atgccactgg ataaccaact ataaggcata ttaatgtagc tcctcctgct aaaagaatag
     5161 aacgcccaaa aactttcata tatatagggt caattacttc tttataattc tcaagtgtaa
     5221 aggcataacc tccacctgat tcttttgtaa aactaaaaaa tactactaat attaaaggaa
     5281 ttattacaaa tatcgcactc caaactacat atggatatgc taaaaaagat tttcttttca
     5341 tcttagttat tctcttttct cattatatgg atatcttctg gatatatatc catgcctagt
     5401 tcagtcccaa cctcagcatt tttagtatta tgaagtatcc acaatcttcc attttcattc
     5461 aattctatct cgtaatgcac tcctttaaac actgtagatg taactttccc ctttaacatt
     5521 ccttcttctg gctttaccat ttttatatct tctggtctta ttactacttc tatattttcg
     5581 tttttttcga atcctttatc aacacactca aaatctctat tacagaaatt aactttataa
     5641 tcttctatca taattccatc aaatatgtta ctttcaccta taaacttagc tacaaatgaa
     5701 tttttaggtt cattgtatat atcttgtggt gttcccattt gttgaatttt gcccttatac
     5761 attacaacaa tagtatctga catagtaaga gcctcttctt ggtcatgagt tacaaaaaca
     5821 aatgttattc ctaatttttt ttggattctc tttaattcta tttgcatctc ttgtcttaac
     5881 tttaaatcaa gtgctccaag tggttcatct agtaataata cctttggttc attaactaga
     5941 gctcttgcta ttgcaattct ttgttgttgt cctccactca atgattcaac ttgtcgtctt
     6001 tcaaaacctt ctagtgcaac aagtttcaaa acctctttta ctttagcatc tattacatct
     6061 tttggagtct ttttaatttt taatccaaaa gctacatttt cataaacatc catgtgtgga
     6121 aataaagcat atttttgaaa tactgtgttt acttgtcttt catatggtgg caaattattt
     6181 atctccttac cttcaaataa aactttacca tcatcagcat actcaaatcc tgctattatt
     6241 tttaatgttg tagtttttcc acatccactt ggtccaagta atgttagaaa ctcatttttc
     6301 tttatatcta aattgaaatt atctaaaaca gtaagttctt cataatgttt agatatatcc
     6361 cttaattcta ttatattttc taccaattat taaacacctc tattctaaaa tgatggtggc
     6421 gtacttaccc aaatcacttt tgctgtagtt ttgccctcgt ttgatatata gtgatttgct
     6481 ttcggtctaa agtaaaaact ctctcctttt tttactttat ttttcttttc gcctatatgg
     6541 acatatattg aacctgctaa tacatatcca aattcttcac cttcatgtgg cttttcttct
     6601 ttatactgtc ctcctggata caatgttatt attattggct ccatttcatt tttttgtgaa
     6661 tttggaatta accactttaa tttatatttt aaatcttcat cttctgtttc aaacatatct
     6721 tcttttgtaa aactgattct ttcatcatct atttcattaa aaaattccct taaatttgta
     6781 cctagtattt caagtatatc tataagggta gctatggatg gtgatgttaa gttattttct
     6841 aattgagaaa taaatccctt tgacaattca catctattag ctaactcttc ctgggtcagt
     6901 tgtttttctg ttcttaatct ttttatcttt tcacctatat ccatgttgca cctcctagtt
     6961 tctgttttat tttactaaac attttgttta ctaaacattg agtttaaaat ttctaaacaa
     7021 cataggtaat tatatataaa atttggatat aatacaagag tttttttgac atttttttat
     7081 ttttttattt agaataaata caagcatttt aaaggctttg gaaaatacac agtttaaagg
     7141 tgtttatatt aatatctata aagtttagta tctctaatta taaattaatt cattcattaa
     7201 atagagaata agacttctag tactaaaaaa atattgaaca aataaaaaat tgctagaaat
     7261 ctatatttta tagtgttcta gcaatcctca tattttaata ttcttttaat attcaattta
     7321 tctatctaaa caaaatgtta atttaaaata cttaacattt cgttgaattt attcttaaat
     7381 tacttaacta tattttgaac agcagctttt ttctcaccac tcatatacac tttgataaat
     7441 tttatatcat ctactataaa tctatgttta atcttactac aatctaattt aaataagcca
     7501 aataccattt gtgtgaaata ccccaatcca aatgcactta ttatagttcc tataccaact
     7561 tttccaccta ataaaaatcc tactattaat gctccaactt ctatagtacc tcttattaat
     7621 ttaagtgatt ttcctgtttt cttttgaatt gcaaccatca aaccatctct aggtccactt
     7681 cctaaaccac ttcccatata aaataccatt ccaacagaca aaagaaccaa tcctaatatc
     7741 aacataatta ttcctacaaa taaactatca gcctctggaa ttaaattact atataaaagt
     7801 aaatccataa agaatccaat tagtagcatg ttaaaaacag ttccccatcc tacattttct
     7861 ccaagaacga catcagcaat aactactata gagcctacaa ttatagaagc agttcctata
     7921 gttattccta tatgatttga aagtccttga tgaaatacat cccagggaga taatccaagg
     7981 ttacaattta tcgtcaatac tataccaact gaacatataa ataaaccgac tactagcttt
     8041 aaaaatttcc ataatacttt ttgcaacaaa ttctacccct cctaaacata atttaactac
     8101 tcactctaaa tatcaaaagc cagcagtttt cttacaaaaa tcttatttta ttaattttat
     8161 aatatacttc tatgatacat tattttatgc taaataacta ccattttatt acaacttagt
     8221 tacaattttt gtcattattt tttggtgaag taatttatac atttatccaa ccaatttcta
     8281 tccttcaaat gttttcaatt tcatttatat tatttataaa tgaaaaaaac tgtctaaata
     8341 taagttatta tacttaaac
//
LOCUS       DAMJZE010000165         8347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJZE010000165 DAMJZE010000000
VERSION     DAMJZE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.169"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(130..1806)
                     /gene="bclA2"
                     /locus_tag="PC541_003123"
     CDS             complement(130..1806)
                     /gene="bclA2"
                     /locus_tag="PC541_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2840564.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2184..2723)
                     /gene="hpt"
                     /locus_tag="PC541_003124"
     CDS             complement(2184..2723)
                     /gene="hpt"
                     /locus_tag="PC541_003124"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2840565.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3051..4358)
                     /locus_tag="PC541_003125"
     CDS             complement(3051..4358)
                     /locus_tag="PC541_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2840566.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4783..6558)
                     /locus_tag="PC541_003126"
     CDS             complement(4783..6558)
                     /locus_tag="PC541_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2840567.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6555..6692)
                     /locus_tag="PC541_003127"
     CDS             complement(6555..6692)
                     /locus_tag="PC541_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840568.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6770..7339)
                     /locus_tag="PC541_003128"
     CDS             complement(6770..7339)
                     /locus_tag="PC541_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2840569.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7523..8158)
                     /locus_tag="PC541_003129"
     CDS             complement(7523..8158)
                     /locus_tag="PC541_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2840570.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2384 a         1662 c          958 g         3343 t
ORIGIN      
        1 ttggtatatg aaatcatatt gtttattatt ttatagtctt ttcatcatat aaacctatta
       61 aatattattc tatttattgg tatatgaaat catattgttt attattttat aatcttttca
      121 tcatataaac tattgtattc tataaactga tacatatcca gctgcagaag tacctgtcgt
      181 gcctgaacca aatgtaaaac ttaaagtaac agctgctgct ctagcatcaa ctaaaaagct
      241 taaacttaca cttacatctg atgaagtaag tgtatttact gcttttgcaa taaagttatt
      301 ttgagctact ccattaaagt tagtagtaat tgacataaat cctgcaagtg aaagtgtggc
      361 acgtataccg aaagatacat tgtatatccc tccaactgca agatttatag tactacttgt
      421 tggaaaagtt atattagttc catttataaa tgatgaatta aatgtcacta atgtattatt
      481 cactaaactt gaagaggaca ctgtaaattg tgcattgttt gctgctaaca ctcctgttgc
      541 tccggtcgct ccggttggac ctgttgctcc atttgctcct gttgctccgg tcgcccctgt
      601 tgctcctgtc gctccatttg ctccagttgg gcctgttggt cctatcgctc ctgctactcc
      661 atttgctccc gttgctccta ctgctccggt tgggcctgtt gctccatttg cccctgttgc
      721 tcctgtcgct cctgttgctc ctgctactcc atttgctccg gttggacctg ttggacctat
      781 tggacctgct actccattcg ctccggttgc tcctactgct cccgttgggc ctgttgctcc
      841 atttgcccct gttgctcctg tcgctccggt tggacctact gctccatctg ctcccgttgg
      901 gcctgttgct cctactgctc ctgttggacc tattgcccca gctactcctg ttgctcctgt
      961 cgctcctgtt ggacctacca aaccatttgc tccagttgct ccggttgggc ctgttgggcc
     1021 taccaaacca tttgctccag ttgctccggt tgggcctgtt gggcctgttg gacctaccaa
     1081 accatctgga cctgttgctc ctactgctcc tgttggacct acctctccat ctgctcctgt
     1141 tgctccggtt gctcctattg ggcctgttgc cccagttgtt cctgttgctc ctgtcgctcc
     1201 tatacttccg gttggacctg ttgctcctgt atttcctgtt gggcctgtag cacctgttac
     1261 tccatttgct ccggttgctc ctgtatttcc tgttggacct gttattccat ttgctccggt
     1321 tgctccggtt gctccagtta gtccggttgg acctgttgct ccagttgctc ctgtattccc
     1381 tgttggacct gttattccat ttgctccagt cgctcctgta tttcctgttg gtcctgtaga
     1441 gcctgttatt ccatttgctc cagttgctcc tttgtttcct gttggacctg ttattccatt
     1501 tgctccggtt gctcctgtat tccctgtcgg acctgttatt ccattcgctc cagtcgctcc
     1561 tgtatttcct gttggtcctg tagagcctgt tgttccattt gctccagtcg ctcccatatt
     1621 tcctgttggt cccgttattc cattcgcccc ggttgctccc gtatttcctg ttggacctgt
     1681 tattccattt gctccggtcg ctccagttgc acctctaggc cccgtcggtc ctgttggacc
     1741 agtagcacct gttggccctg ttggacctac atcttgatat aaacttggac ctgaaatatc
     1801 actcatataa ttaatcctcc ttttttaaag ttagagtatt actctataaa tattgagtat
     1861 tactctatat aattatatga atcgacaatt tcttatgtga taatgtatct atttgcttat
     1921 atcaacatat atgctttgat tcttgaattt cattactttt ttgtaaaatc ttttacaatt
     1981 attagtactc ttttacaatt attaatacaa ttaaaaaact cattttccat caattattta
     2041 aaattttttt attatgttat aaaaaataga taattaaatt gcataaaatt aatttaatac
     2101 tactttaatt actttgtaaa taaaaaaacc tcaacacgat taataaaaat catattaagg
     2161 catccacaaa attatataat tgcttatcta tcttcatcag taacattgaa tatataagga
     2221 acatttctat aataatcacc aaaatcaaac ccatatccaa ccacaaactt atcttctatt
     2281 tcaaatccac aataatctgg cactaaatcg acttttcttc tgcttggttt atccaaaaga
     2341 acacaacatt ttaaactagc aggattttta gcttttaaat gttttaacac aaaatccata
     2401 gtaagtgcag aatcagtaat atcgtctaca accaaaacat catatcctgc tatatcagta
     2461 gtcacatctg aaactacttt aacccttcca gtactttctt cattgtttcc ataactagat
     2521 gtagtcataa aattaactct aagtggtaaa tttatatttc taactaagtc tgcacaaaat
     2581 ataaagctac cttttaatag agaaactacc ataagatttt tatctttata atctttttct
     2641 ataattgctc ctaattccct taatttatct ttaatttttt cttctgaata caatacttcc
     2701 catttttttg tttctatatc cataaaaaga cctccaattg taataatcct tcttaatttt
     2761 ataagagatt gctcactttt tcaatactct tattcaatat tagtcaatat tattgagttt
     2821 ttatgttcat tcaacaatta ttaacaatgt atcttatatt ttaagtagtt catatatcca
     2881 cactaaatta catactatat attttagaca aaaaaagaaa aaacttgcac taacaagttt
     2941 tttctattat acataaattt atacataatc atattttatt agagggtact aattaaattt
     3001 atttatgcta tttttgttat acctcataat acaaaacata ttataaagta ctagtcaact
     3061 ttattcatat cattcattac atttaagatt atctcatctg ttatagacat acctttgtca
     3121 cttactctac ctaagttttg tatactttgc tcaacacttg aaccaactat accatttagt
     3181 ggtggtacaa aacaatcttg cttagctatt atagcacttt gtatagctgc agatgctgca
     3241 gaagataatt ttaatgcaca accagctttc gcaccgtcac atatcattcc acttaagtca
     3301 gctatcatat tttctatagc tccttctatt tgcttttcag taccattcat aagccaagag
     3361 ataccagcag ttgctccagt agaagcagca acaccacatc cacatacagc agataatctt
     3421 cctgtatagt tcttaatata accagttact agatgagata tagctaaagc ttttgctagt
     3481 ctttcatcag attgtgggaa ttttttatta taagcaacga ttggaagaat tgctgttaat
     3541 ccatgatttc cacttccatt tgaactcata acaggcattt ttacccctgc cattcttgca
     3601 tctgaagctc cagcagttaa catcattgca taatttataa catcattacc taaaagacct
     3661 tcttctatag actttttaat tccatacccg acaccaatac cagttttttg ttttaatcca
     3721 tattcagcca tctcttcatt catttttact ccatctagta aaaattctat atctttaaaa
     3781 tcaatttctt ctacattttt tattaattct tgtatagtta ctgtatccat taaactttca
     3841 gctttttctg ccgcatcatt tgaagctgat acttttggtt cattgtctaa taatacttct
     3901 ccatcttttt ctaagaacgt aaaattatca tgtttagttc ttattctcac tttagcaata
     3961 tgattttttc cttttaacac aacttctata aatactttct ctcttgtatc agcaggtgtt
     4021 attgatagtg gagtattatc catataatcc tcagctattt taacttcctc ttttgttaga
     4081 gtttctaaca cacttaatcc attttcagaa tgtccaccta ctatacctag agctgctgca
     4141 atttttaatc ctaatctttc agtacctggt atacctacac acataccatt tttatagata
     4201 ctaggactaa ctaacatacg attttcaact atttcttctc ctaataattc ctttgccttt
     4261 gcacatgcta gagctaaggc tactggttca gtacacccta ctgctggttt aacttctgcc
     4321 tttaacattt ccactaattc ttttcttata tctctcatta tatataaacc tcccaattaa
     4381 gtttagattt agtactcaaa taattagtta aataatttct tatctatatt tatagcaaaa
     4441 tacatgccat attttttata tctttttata tttttagtct tatattctat aaccattggt
     4501 atgactgcct ctatttatgt atttttttta tttgtataga gtatttatat tctttaaaat
     4561 ggagaaacat atgacaattt cgcaaaaaga caaaaaaaag tctcaaaatg agattctata
     4621 tcattttgag acggctcaaa tatattattt tttaacttat aaaaaccatt ttttaattca
     4681 taaaaaaaga ttctgcaaaa cacttatagt ataagtttaa agctttcttt aaagttatga
     4741 atttagaaaa atttaaattt tatatagctt tttattatat tactatgaaa ttatattgta
     4801 tttttcaatt tttctatata atgtagctct tgatattcca agtgctttag ctgttaattc
     4861 tttatctttt ttataatctt tatacttatt taaagctttt attatctcaa tcttctctag
     4921 ttcatcaagt ggaattatcc tatcgctttt aacttctaac tcttctacag aggtattctt
     4981 aattttttga ggaattacat tcattgtgat tattgaagaa gaagacatat tcacactata
     5041 ttcaataata ttctgcaact ctcttatatt ccctggccac ttatattcca tcatagcatc
     5101 aacaaccctg ctatctatgt caataacatt tttatctaat ttttttgaat actctttaac
     5161 catatgcttg attataagtg ggatatcttc ttttctctct cttaaacttg gtaattttat
     5221 aggtataaca tttaatctat aatataaatc ttctctaaaa gtgccatttt gaaccattct
     5281 ttctaaatct ttatttgtag cagcaataat tcttacatct attgaaacat ttgatttact
     5341 tccaatttta ttaagttcct tttcttgtaa tactctcaat aactttgact gtaaatgcaa
     5401 actcatatct cctatttcat ctaaaaatat acttccctta tgagctactt caaacattcc
     5461 aagctttcca ccttttttag ctccagtaaa agctccctct tcatatccaa ataattcact
     5521 ctctaataaa ttatccggta tagcagcaca gtttacagct ataaaaggat tatctactct
     5581 attgctatgg ttgtgtatgg ctcttgcaaa tagctcctta cccgttccac tttccccagt
     5641 aataagtact gttgatgttg atttagaagc tattagggct tcctttttag aatttcttat
     5701 agctacactt tctccaatta tgttttccaa ttttattcta taatctttat tcatcttatt
     5761 gtagtttttt atagcatctc ttttatctat aaaatcaata acatatccct ttaactcatt
     5821 ttttaatact attttattga tgttgtatat tcccttcgaa tcatatccgt atcttttata
     5881 aaaaaaacta caggatttgt ccttcttaaa gttatttata gaaggtttct ttataaaatc
     5941 taactcgaca aatatgtttt tgccagttat actatctttt atattgaaaa gctctttaaa
     6001 ttttgaatta tatttatcaa tttttccatc cacatcaata gacacaacag ctttgtccat
     6061 attgtttaat agtgtctcta attttttctt ttctaattca agctcataag ttttaatttg
     6121 tgcttttaat ttattagata ttaggtctgc cattttgcct aaaaagttta taagaccact
     6181 tttattattt tctataattt gtgcttgttc ttttgtaaag gctataaggc ctataacacc
     6241 atgagaatcg ccatcacata taataggaca acacacctct gcaaactcta cacaagcccc
     6301 acttttatag cattccctac atatatcact actctttggg tcatcaatta gtattgtaat
     6361 ctgttctgta agtgattttt taaaagccgt atatttatct actttctcac ctattttgtc
     6421 tatatatact cctgtacctg caatcctaaa taactcctta tttactatag ttacatctat
     6481 attgagtact gatactatag cttcagctat attttgaata tcagatttta tatattctaa
     6541 acctatcata atcatcattc cttatttttc tttagcataa gtatttgtct tatttcttta
     6601 agaatcaagt atatcttatt taaagtaaat tggattgaca tcaggattac aaagagcata
     6661 acaattatta ttaactcata atttatttcc atctaaacat ctccttataa caaaagacta
     6721 ggcttaacct agtcttttat attatatcca tatttatatt ttaactcatt tatcttcttt
     6781 ttcccacata aatatatttt aatttatttg actctttaat cataccatca aagtttaatc
     6841 ttgaaaatat attaaatact tcatgtggat agtgaatctt tgcaacagaa ccacaaaatt
     6901 tatgtttaag atagtttaac ttaactttta aattttttaa atttaaatcc tctaatataa
     6961 cctctccatt gccctttaac actctttgta tttcagatga agctttttca caatctgtaa
     7021 taacattaaa ttggtcaact attattatct tgttgaactg attatcatca aatggtagaa
     7081 actctaactt acctttaact acattgcaat tttcattata cactagagaa taacagacat
     7141 cttcataatt actattctcc agtatagtta cagaagttcc aagacctctc aatctcttgc
     7201 ctaggtttcc tatatgacca acaagcaata ttttatcacc tacattatac tcaattgtat
     7261 ccaaaaaaca ttctaattca tcagatgtat atttagcttt aactggctgt ttatttatct
     7321 gacataatat gcttttcaca atgcttcacc tcttcatttc ctcaattata aattatgtac
     7381 agattacatt ataataaatg gcgagcaact attcaagaga tatttcatta gtattttttt
     7441 aaacaatctt tatacatttt aatatgatat tataccaaat ttattaggat tttattcata
     7501 aaaatcagtc tattttttat gtttattcat tgttattttc atccaatttt tttattttcg
     7561 atatggatac ttcatatgcc atttttttga ccacattatt ttcatctatt tttttctcat
     7621 attctctact ttgaacccta ccccaaagct gtattctatc accaaccttt agattctttg
     7681 caaatttagc atttctaccc caaactattg atggtatata atcagattta ttataaggtc
     7741 tgtttatagc aactaataaa tcggttattt cccttcctaa aggtgtcttc ctatacacag
     7801 gttccttaca tacatatcca tctaaaaata tactattagg gtctttattt tcctcgtcta
     7861 tactttttat ttctctgaca aacactgtaa gcactaatct atttttatta tttaatgttt
     7921 tgttatatga tcttaattgg cctacgacat tgactaaatt ttgtttatca aggtcaatat
     7981 cttggagaag tctttctgat acagtcattg gaagtatatc aaaagaatcg cttagtctgt
     8041 ttatttttat tttcatatta taaaattttt ctccaaatat ctcatggcta aattctagct
     8101 tattgtctaa ctcccctctt aagtttacta cgttattctc atctttaaca gctatcatat
     8161 tttatccctc cacaattgtt aattcttata tttctggtat cagcttatat aataaatata
     8221 tttataaggg atgtatttta ttacaaatct ttgattgctg tttaaaagtt tttatttagt
     8281 tataaatttc atttaatatt tcacttttat aattataaaa ttattatctt tttgtaccac
     8341 atatact
//
LOCUS       DAMJZE010000166         8310 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJZE010000166 DAMJZE010000000
VERSION     DAMJZE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.170"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(118..987)
                     /locus_tag="PC541_003130"
     CDS             complement(118..987)
                     /locus_tag="PC541_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2840571.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1084..1941)
                     /locus_tag="PC541_003131"
     CDS             complement(1084..1941)
                     /locus_tag="PC541_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2840572.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2156..4174)
                     /locus_tag="PC541_003132"
     CDS             complement(2156..4174)
                     /locus_tag="PC541_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2840573.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4445..5221)
                     /locus_tag="PC541_003133"
     CDS             complement(4445..5221)
                     /locus_tag="PC541_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2840574.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5278..6249)
                     /gene="asrC"
                     /locus_tag="PC541_003134"
     CDS             complement(5278..6249)
                     /gene="asrC"
                     /locus_tag="PC541_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2840575.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6262..7053)
                     /gene="asrB"
                     /locus_tag="PC541_003135"
     CDS             complement(6262..7053)
                     /gene="asrB"
                     /locus_tag="PC541_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2840576.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7054..8061)
                     /gene="asrA"
                     /locus_tag="PC541_003136"
     CDS             complement(7054..8061)
                     /gene="asrA"
                     /locus_tag="PC541_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2840577.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2672 a         1505 c          755 g         3378 t
ORIGIN      
        1 tgttttttaa taaaaaatca tgctacatca atagtaagca actattaatg tagcatgatt
       61 ttttgtatct aaataatctt agtatttacc tatcagtata gattgatata tcaaaaacta
      121 tagtcttcta tattcttctc tttttagctt ttggttactt agatagtaat caatttcatc
      181 aattagacgt tgtttatcat caggtaatac accttttaaa ttagcataat ttgatgcatg
      241 attacttcta aatacaattt tttcattagc atctatattc tttatcatct ctttaatttc
      301 ttgtaacaca gccttatcct ctagtattgt aaactctcct gccttaagta aatcgtaaag
      361 ttcagtatta ggttctacca ttaaactaag tacacccaca taatctggtg aaattgctcc
      421 tagcattttt cctgtatcaa ctgcatgctg atgtgttttt ttagttcctc caagacctgc
      481 tattacagtg gctgataatt taatcccact ttttttaact tttctaccaa cttttattaa
      541 ttgttcacta ctaaaacctt tcttaatatc ttctaatact tcattgcttc cactttcaag
      601 ccctagataa atcattttaa caccaagttt tcttaattct ttaagttcat catcagattt
      661 tttctctata gatttaggag agccgtacat agtaattctt tcacactctg gaaaaacttc
      721 ttttatatat aaaataatat ctcttaattt ctccattggt attatcaatg catctccatc
      781 tgctaagaat attctttcta catgcttata atatcttcta aatatatcaa tttcattttt
      841 tattacttct agtggcttta ttttaaattt tttagatttg tacatactac aaaacgtaca
      901 tttgttatga gaacatccta gtgtgacttg tattataaga ctataagcct cacttggtgg
      961 tctgtaaagt ggcatatcgt attccatatt aaaatccccc ttgtaataaa atattcattc
     1021 tattatatta ttaatattta ctttaaataa taacttataa aattatattt tttaaatttt
     1081 agtttaagat atattcttta aaaatttaat gatactattg gcagtaatat taagttgttt
     1141 ttcttgactt ataatagcat cattatctcc cttttgtttt ccataatctc caaattgtgc
     1201 atgatttcct ccttcaatct caacataagt tgtatcatca ggaagttttt gttttgattc
     1261 tacaagactt ttaaaattga ctactccatc tttgctcccc catatagata taactttttt
     1321 tcctaattct ttcaactcat cacccattgg ataagaagct aaaaatacaa cgccttcaat
     1381 taatttatta tcacgtgcaa aatttgatgc tgcaactcct cctaatgaat gacctcctat
     1441 tacccaatgt tctatgttgt tgtaagaatc cataactttc tgtgccttat tagttccaag
     1501 tatagctaga ttaagtggca tctttacgat tacaacttca tatccacttt ctgctatttt
     1561 tcttgcaagt ggagcataag attctatttc tactttagct cctggataca aaattaaacc
     1621 tttagaaact tttcttcctt ttggtgtaaa tgaaatgttt tcatttacag ttacttcaac
     1681 tttactattg ctgactagag cttcttttgc cagcttttga ggttcatatg ttttatttat
     1741 ccatatgaaa aagccaagca aaatcaatac aaatagtata cctattccct taaatatctt
     1801 attctttatg ttaaattttc tattattatt tttatcatct ttgttctcaa cctcatcata
     1861 aagtaaaggt ctctcaagtt ccatagttat actaaatgta tcatatttct tattacgctt
     1921 tacctttaca tctttactca taaatctaac ccccataata tttgaatact attatagttt
     1981 atcctaagta aattgttttt tcaaccaatg aaaaaaatca atattttaac tattttcata
     2041 taaaattcat aaatatatgt tttttgtttt ttatatatta ttttctttta gtattttata
     2101 aaacaaatag ccatatactt tattttaaat atatggctat tctataattt aatatctaag
     2161 ctgcttcttt tattgaaact ttagttatat tacttattct atacatttct ttgacaccat
     2221 ttagagtttg cagtaagttt agacatatta tcttgtcacc tagactttta gtatttataa
     2281 tactctctaa aattacactt cttccttcat catacttatc tacattgtaa tttgaaatat
     2341 aattagtatc tctcaatatt ttttcaacat cttcatcttt tattttattt ttattaacat
     2401 ttatatatag attatacata ttacttatta gtaatttctg attttctaaa aaatcctctt
     2461 gatgtttatc tttatatata gcatttatca acataagtct atcttcaata agtgatggta
     2521 tcaataataa attttttata tagtaaacat cagctacatt ttctatagac ttattgactt
     2581 catcaattat atttttaact attcctttat acatttctat aacatgttga tagcctgttt
     2641 tagcatcata tggtaatata aacttattta ctattaatgc aataattgct cctaatgcta
     2701 catatgaaat cctgcttatg gttaaaacat ttgggtctcc cataactact gctgaaccaa
     2761 gagctgaaac agttacacat accattaatt ttctataatc aactacataa gagtttatat
     2821 accctgctaa aagaactatt aacgacctta atgtactatc ttttattatt gaaaataata
     2881 caataaatat tacagctcct ataaatgtac cttctattct ttttctagac ctttgtatac
     2941 aattttctgc gtaaggttgt gttaatgaaa agacagttaa cattatccat cttccatctc
     3001 ttaaatggaa ataatccata ataaaacccg ctacagctgt agctaaagca atctttactg
     3061 cataagaaaa tcttatagaa tttaaattaa agtttttctt atatattgat agtcgtttaa
     3121 attcatgagg tatctttgat tttctttcag ctttcttttc gtctcttgat tttacttttt
     3181 tataatctac taaaaactca tataaatttt ctaaaacact ataaatttct tttaaattta
     3241 tatttttagt atcctgagca ataacataat ctctaaatcc taatatctct ttatcagtta
     3301 tggtatcttt attttctaaa cacatcttaa tatagtttat ttctttcgtt gctacaacta
     3361 gaacatctga tttactattt atgctacttt cttctaaaat attttttata tcttcatctt
     3421 caaattcttt actatccttt ttgtatcttt ctaggattaa acttattctt tctaatgaaa
     3481 ataaaatatt cattatgtct atacctctaa tactgatata aaagtcatct tttctattat
     3541 caaaaataac cattttcaaa tttttagata tagaatttat tctataatta cagctatcta
     3601 tttttctcat actagattta attatagaac tatcatcatt aatagtattt tttaaaagac
     3661 taattttttc taataattca tcacatatag atatcagttc tttatctccg acatttttca
     3721 tcctatttct attcataaca aattgagcaa tcattattat aactgctcca acagctaaag
     3781 ccgacaatct cataggaaat tcactttttc ctacaggtat acttaccata aatatataca
     3841 ttagaccaaa gggtacatat actgctcttt ttaagtcata actaaataca taccctatta
     3901 taaataatgc tataaaattt aataaaactc ctaaatacat attttgtact gcaaaaaatg
     3961 atagtattcc taagattata tttattgcta aatacttcag taaattttta attggagaaa
     4021 tagttaaatc tctttccaag agcgttagca tagctgttac tatacctaca cctataagtg
     4081 tatttttact tccaaataaa tatataaata tagaaataaa tgctattata aaaataaaca
     4141 actttgtttt tgatattact agtttctttg tcatcatatc ccccctaaaa atttcaacaa
     4201 gaccaatttt atcatatttg ttatcttatt caaaatgcgc ttttttacgg taggacttta
     4261 acttttttta tatttagaac tatataattc atatataaac gtatttttta tatatataac
     4321 cgatatattt tcgtatgctc ccatattttt tttgtttcaa atattttttc aaaaatataa
     4381 aaataactct ctcaacaata ctaaaaatgc atttatcaag agagttataa tttatacaat
     4441 aaatttaatt aagcttttct ttacctaata tataagttcc aagacccata aataaagctc
     4501 ctccaacaat atttccaagt gttacagcta ctaaattata aatagctcct ccaatagtaa
     4561 ctgtatttat tgttggtgaa aataatgaaa cactataaat tgtcatattt gctacactat
     4621 gctcaaagcc tgatgttata aaagcaaata aacataagaa tatcataatt atttttgcag
     4681 tatcactatt agttctaaac gaacacaaca ctgatacaca tactagaata ttacataaaa
     4741 tccctctaaa gaaaagagct gtaaatggca tggatgcttt actagctgct gtatttgcaa
     4801 aaaattcagc tacaggtcct ttgtctatta gaccagttcc tacaaatata atgcctaaaa
     4861 ccaaagctcc gattaaatta cctacccaac tgtatgccca tattttagaa gtatctttta
     4921 tacttacacc tttatttaac ataccagcag acataaccat attgtttcca gtaaataact
     4981 ctgttcccgt cattataacc aaacttagtg ctattgcaaa tgataacccc attactatct
     5041 tagtcatagg tgaacctgca tcagtcaaaa gtcctccaat agtaaatatc aaaagtatac
     5101 ctatacctac ataaagtcct gcaaacgcag aagataccaa atattttact ttacttgtgt
     5161 ttaataaatt tatcttattt atagctgcat tagttaattt atccaaagtt tctttatgca
     5221 taattccctc ttccttctat taaattttaa tttattcaaa tatatgtttt actcaattta
     5281 tagatattta attccactcc agtatatgtt ttcgtacatt ttagttattt ctggaagctc
     5341 tacaccttca agtgcccatt ttttaaattc cataaatcct gttctatcaa ttatatatcc
     5401 tatatgttct tttcctcctg gtgcatcttt atctatatac ttttctacat atctataagt
     5461 attaagtatt atctttatta tgctgtcctc atctacccat aaaagaaaat cttctgctag
     5521 tctagggttt ttctttcctg ttctacccat tatagctaat ctatagtatt ttttctcatc
     5581 tctagtccat gcatttgttg ggcaatttag cacacactct ccacagccta tacacttatc
     5641 tttatctctt accaccttat tattttctgt ttttaatgct ccaacagata tcttagaaca
     5701 tttttttaca catgctccac aatttacaca tctatccttt tcatatattg gtaaagccat
     5761 accaataatt ccaaagtcat tcattctagc ttttatacaa tcatttggac aaccagttaa
     5821 agccacttta aaatgtaaat catttggaaa tatagctttt tctattcttt tagcaaattc
     5881 agttgtatta tattgagcct ttggacagac tttatttcct atacaagctg ctatatttct
     5941 tgtacctgct gctggataac ctgcgttttt ctcactttga tttatattca ttttttcaat
     6001 aacaggttgt ataattttat ttatttcttc catatcttcc atatttattc ctaatatttc
     6061 aaacccctgt cttgtagtta tgtgcacctg tccattgccg tactcacttg caattttaga
     6121 aactacagct aaactctcag ggtcaataag acctccaggt actctaactc ttaaagaagt
     6181 ctcatatttt gtttttgtaa ttctataagc atttttcatt acttttttgg tatttaaatc
     6241 tcttatcaca aacttcaccc cctaatcaat aagattttta gcatatgaat aatcaaatac
     6301 tggtccatct aaacatatgt aagtatcatc cattttacaa tgtccacatt tacctattcc
     6361 acaacacatt tttctttcat aagaaaccca aatgttcttt tcatctaaat ctctttttaa
     6421 aaattcttct actgtaaatt tcatcatcat tggaggtcca actacaacta tagaagtatt
     6481 gtttatatct ttcatatcca attctggtat gtattttgtt actaatccta cattaccact
     6541 atatccttcc tctgctccat ctacagtaac taaaatatca agttttttac tccatctctt
     6601 taaatcatct gcaaataaaa tatcttttgg tgatttaaat cctactatta atttaaactt
     6661 ttcgcatttt tctttattat tataaaaata ttcaactatt cctcttacag gggctaatgc
     6721 acttcctcct gccactacta caatttctct tccttcataa agggatacat caaatccatt
     6781 tccataagga cctcttataa aaaaagaatc tccttcttta taattaaaca actcgctagt
     6841 tacttttcct acatttctta ttgtaaagtc aatataatcc tctccatatc ctgatactga
     6901 tattggactt tcaccgtatt taggtataga tatctcataa aactttccag gcaatacacc
     6961 ttttgtattg cagtttactc taaaagtcca ttctttatca gtatgtttaa ctatatctag
     7021 tatttttgca cttactggta tatatgggtt catttatact tcctccttac taattactaa
     7081 atcatttatt ttttctatac actttgaaaa tgatatgtat tgcggacagg catcatcaca
     7141 tcttccacag ccaacacaca tatgatatcc gaatcttttt ttaaaatcat gtattttatg
     7201 catagtttta aatctcattc tttcgccttg tttttttcta aaagaatttc cacctgctat
     7261 atctgtatat ccatctactt gacaagaagc ccaaaccctt cttctctcac ctacattttc
     7321 gttttcctta tagaaaatat cctgcattgt aaaacatgaa catgttggac atacaaaatt
     7381 acatctacca catcctatac atcttgtatc atattcatcc catattgaat ttcctatcat
     7441 ttcatttgaa tcaatattct ctggcacatc tacagatatt aaattatcag ttacataatc
     7501 aacattaaat tcagttgctt ctccattaaa tacttcaaat tcactatcct ttatatctaa
     7561 gtacagtgta tctccttcta tatttaatcc aattgaataa ttatcagttt tgttagaatc
     7621 catgctcaca caaaaacagt ttctaaaact ttccttacac cctacaagtg caaactttac
     7681 tttatctctg atatttttat aaaaataatc tttttcttca ccattattta gatatatttc
     7741 atcaagtctt tttataccat ttatgtcaca agctcttaag aaaattaaaa ttttcttgtt
     7801 gtctaaatcg ctttccttaa actctttttc agtaaaataa aacaatactt gatttatggg
     7861 aagtacaact tcttttggtg aaaagtttgc tttcttagca aattccattt cttcaaaagc
     7921 gtttacctcc ttgtatctta taacatcagt atctgaaaaa gtacctttaa atgggattaa
     7981 aactggtgca aatatcttgt agtctttttt tagataattc agaccttcat tgaacttaga
     8041 tgaatctaat tttagtttca tttttatcac ctttcttata ttttagtttt acttacttgc
     8101 tatcttgttt aaatcttaac actttatttt ctatgcttcc gtgataatta tcacagtttc
     8161 aaaaataata aaaaaaatat taatggagtt tattaagttc tgttaatatt tttttattat
     8221 ctacattatt cgatttcaaa tctaatcact taaatacaat cattaagtac aataaaacta
     8281 ctatctactc aattttttga ataaaaaaga
//
LOCUS       DAMJZE010000167         8275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJZE010000167 DAMJZE010000000
VERSION     DAMJZE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.171"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            56..1042
                     /locus_tag="PC541_003137"
     CDS             56..1042
                     /locus_tag="PC541_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2840578.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1211..1359
                     /locus_tag="PC541_003138"
                     /pseudo
     CDS             1211..1359
                     /locus_tag="PC541_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1378..2859
                     /locus_tag="PC541_003139"
     CDS             1378..2859
                     /locus_tag="PC541_003139"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2840579.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            2885..3856
                     /locus_tag="PC541_003140"
     CDS             2885..3856
                     /locus_tag="PC541_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2840580.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4112..4714)
                     /locus_tag="PC541_003141"
     CDS             complement(4112..4714)
                     /locus_tag="PC541_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2840581.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            4995..6206
                     /locus_tag="PC541_003142"
     CDS             4995..6206
                     /locus_tag="PC541_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2840582.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6274..6471
                     /locus_tag="PC541_003143"
     CDS             6274..6471
                     /locus_tag="PC541_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2840583.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            6505..7476
                     /locus_tag="PC541_003144"
     CDS             6505..7476
                     /locus_tag="PC541_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2840584.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7663..8040
                     /locus_tag="PC541_003145"
     CDS             7663..8040
                     /locus_tag="PC541_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2840585.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3358 a          869 c         1334 g         2714 t
ORIGIN      
        1 tttaaatata taatataatg taataaaata tatcaaatgt agaaggtgat aacctatgaa
       61 aaaaataaca ataaatgata ttgctaactt ggcaggagta tctaaaagta ctgtatcaag
      121 atatctaaat aataaagata tcagtgattc tactaaagag aaaataaaga caataataga
      181 tgaatatgga tatgagccga atgcatttgc tcaaagtttg agagctaaaa aaacatactt
      241 tattggtatt ataacaccgt gtttagattc ttttgtaaaa tctaaaatta tgatggcaat
      301 agatgaagaa ctcaaagaat taaaatatac atcactaatt attaatacta atagaaaaat
      361 aaggtcagaa atagatagta tatcaaaatt agctagtcta aaagttgatg gaataatact
      421 aattggtacc gaaataacta aagaacataa gaatgaaatt gaaaagttag atataccgat
      481 agttgtagtt ggacaaaaag ttgatggtat caatagcata gtgaatgatg attatggagc
      541 aggatataaa atgggtcaat atattgctaa taaaggttac aaaaacattg tatatttagg
      601 tgttgatgaa agtgatatat cagttggttt aaatagaaaa aatggagtac ttaatgggtt
      661 aaaagataaa gggtatgatg caaaagtatt ttatactgac tttgaccaag aaacgtctat
      721 acaaagaagt ggagaaatgc tagaaagtga aaatccagat ataataatat gtgcaactga
      781 taatatagca atagcaacaa tgaaagaaat caataagaga ggtaaaaata taccacaaga
      841 tatatctgta gctggatttg ggggatatga tgtattgtct attataagcc ctaaacttac
      901 aacaataaag tttgaaaata aaaatgcagg taaagtagca gcaaatacga tagtaaattt
      961 aatacaagaa agaaaagaac cactattaaa ggagattaaa tttgaattaa tagaaggtga
     1021 aagtacaata aataaaaatt aagatacaca ttacatatat tagtaatgtg tattttttta
     1081 ctaaaaaaac tataattcta aaaattagga aaactttttt gaaagaaata ttgcatctga
     1141 aataaattta tgctaatctt taattaaacc ggtttcgaaa ccggtttaat taaaccgata
     1201 gaatagcaaa atgagtgcaa aaataactga ttgtaacaaa gtatcaaagt aattattaga
     1261 gtacatagga ataaacaaaa tataaaagat gtagcacatt gtgtaataag actaagaatt
     1321 gttttagata gcaataataa agcaaatata aaattataaa agaaaaggaa taaaaatatg
     1381 tacaaagagc ctaaatatag aaccatatta gaatcgacaa aagaagaatt aaataaatta
     1441 agaaatatat ctttaaatga taaatataaa ccactatttc atatacatcc acaacatgga
     1501 ctacttaatg acccaaatgg attagcatat tataatggta agtatcatgt attttatcaa
     1561 tggtatccat atgatgcaac tcatggaatg aagcattggg cttatgtaag ttctgatgat
     1621 tttgtaaact ggaatagaga agatgtagca ttaataccaa tcgaaagtta tgaatctcat
     1681 ggagcatatt caggtaatgc gatagaggta gatggaaaat tgcatatgta ttatacaggg
     1741 aatataaaat atagtgcaga agatagatat gcatatcaaa acttagctat aatgaacaag
     1801 gatggtaaaa taactaaata tgaaaataat ccaatagtaa gtgaaatacc aaaaggatat
     1861 acaggacatg taagagaccc aaaagtattt aaaagaaagg ataaatactt tatgttactt
     1921 ggagcacaaa caagtgataa aaaaggagct ataatagtat atgaatctaa aaactctata
     1981 gattggaatt ttaaaggaga attaaatgta aaaaatatag atgaagattt tggttacatg
     2041 tgggaatgtc cagattatat taatatagat gaaaaagata tattaatatt ttcaccacaa
     2101 ggagtagaac ctaaaggatt tgattatcaa aatatatata atgtagtata tgctattggt
     2161 aatatggatt tagataattt aacatttgaa atagatacaa tgaaagaact ggaaaaagga
     2221 tttgatttct atgctccaca aacatttata aaggactcac aaataatact atttgcatgg
     2281 gctggaatgg gagaagtttt ataccctaca gataaaaaca aatgggcaca ttgtctaact
     2341 gttccaagaa aattaaatat aaaaaataat aagctattgc aaatgccagt agatgaatta
     2401 attaaattaa gatatgatga aacaagtggg caaaatacta taaagaataa tattaatatt
     2461 atagaaaatg atgaaaatgt atatgagctt aatattaata taaaaaatat tgacagcaat
     2521 aaatttgggc tggaattatt ttcatcacaa gatgaaggtg taaaattaga atttaacaaa
     2581 ttaggaaaca ttgtaacttt agacagaagc aactttaaaa aagttttcgg tgttgaatat
     2641 ggaacaaata gaaaagaata tataaatatt gatgaaaata ctaatatcaa agtactagcg
     2701 gatagaagta ttttagaaat ttttataaat gaaggagaag tagtatttac tagtagaata
     2761 tttgctaaag aaaattctaa tcaaataagg gtttatagtg ataaaatagt agagtatgag
     2821 tatactaaat ttaaattaaa gcaaggtatt gaattataaa aacacattat aaggggtaga
     2881 aaagatgaaa aaagtaatta gcataggaga agctttaata gactttatac cgaatcaaaa
     2941 tggtggtaaa ttaaaaaatg tatcagaatt tagaagagta gcaggaggag ctccagcaaa
     3001 tgttagtgct gttgtagcaa agcttggagg aaaatcgagt tttatatcta agttaggaaa
     3061 agatgcattt ggtgattata taatagatgt attaaatgaa gtaaatgtaa atactgatta
     3121 tgtattgaga acaagtaaag caaatacagg acttgctttt gtatcattaa aagaagatgg
     3181 taatcgtgat ttctcatttt atagaaatcc aagtgcagat atgcttttag aagctgatga
     3241 agttaaaaaa gagtggttta ataattgtca tatcctacat ttttgttcag tagatttaat
     3301 agatagtcca atgaaattag cacataaaaa agcaatagaa tacgcacttg aaagtaatag
     3361 tataataagc tttgacccta atataagatt acctttatgg gatagtgagc aatcttgtaa
     3421 aaaggcaata tcagaatttt taccttttgc tcatattgtt aagatatctg atgaagaatt
     3481 agaatttata acaggagaaa atgatataga aaaatcatta cataaactct ttgtggggaa
     3541 tgttgaacta gtattatata caaaaggaaa agatggagta gatgcataca ctaaaaaagt
     3601 aaaaggtatg tgtaaagggg ttaaagtaaa tgcaatagat acaaccggag caggagattc
     3661 ttatataggt tcatttttat acaccttatt gtataaacaa ataacattga atgatataaa
     3721 agaaatgaaa caagaaacgt taaatgagta tttagaattt tctaattatt atgcagctcg
     3781 tagtacaact attaaaggtg cgataagttc atatgcaaca aaggaagaaa taactgaatt
     3841 tataaaaaat ttatagtagg aatgagtaat aggttttgaa ggaagtaaca tgtaaagagt
     3901 ttttaaggat gtttactatg aaaataatta acatataaaa agcaataaag aggctgtctc
     3961 aaaatagaga taagcctctt tatcattgtt gaaatgataa aaaatatttt aattattgtt
     4021 actttttaga agaatatata tcttatttta aataagcaaa ttatgtaaga tttatttata
     4081 ttatcaatac tttacttgga gtattatgtc tttagcagtt atctacttca attgaattat
     4141 ctaaaatata ataaatttgt tttgcaaatt gacgagaatg ttcttctttt tctttattca
     4201 taaaattagg tttacctaaa tcacgttcaa caagcattcc tatatatttt aactgtgaat
     4261 gcttacagta gcgcttcata ccattttccc aaatatcagc acccttttca ggtctatatc
     4321 cacatgttgt aataagtgct actggttttc ctgcccatag agtggggcct ttctcattac
     4381 catagtattt attcattcca tacaccaatc tatcaagtag tgctttcatt ggagaagtgc
     4441 agtaccaaga ataaattgga gtagcaaaaa caattaaatc acacataaga attttcttga
     4501 aaattttctg aacatcatct ttacaataac atccaaaatc aaataagtta tcctgacatt
     4561 ttttacaagc aatacatggc tctattttac aatcatataa ccatattact tcaatatttg
     4621 aattataatt ttctaattcc tcaataaaag gttttaaaat agcagcagta ttaccttttt
     4681 tcttaggact tcccataaga atgcatactt ccatattgat acctccacag tattttaaaa
     4741 tagtttttag tgcttaatat agttttagga tactatgaga atacgttaac aacaatacac
     4801 tttgtatatt tgtaataaaa ttgaaacaaa ttgttttatc aataaaagtt ggaatgtatc
     4861 tataattaaa aatattttag gtatgttttt acatatatgt gtgtagttaa ctatggtaat
     4921 ttattgtaaa attatcttat aggattataa ttgcttaatt tatgattata tttaaaatct
     4981 tggaggtaaa aaatatgtac tcaaatgaag actataaatt tatagaagaa aatgcaccat
     5041 gttatatata tgatgagact aatataattg aaaaatgtaa ggaattgaaa aataatattt
     5101 taggttttga ttttttatat tctataaaaa caaatccata taacaaaatt gtaaaaagca
     5161 ttgcagagca aggttttgga gcagatgctt cttcaactaa tgaagttatg atttcatcag
     5221 aatgtggagt ttctaaagat gatatttatt attcatcacc aggcaaaaca gagattgata
     5281 tagaaactgt tttagataaa tgcataatta ttgcagatag tattacagaa atagaactta
     5341 taaataagat atctaaaaag tataatcaga aagttaaaat aggaataaga ataaatcctg
     5401 attttactat ggatgatgat agtgctaaat caagcaaatt tggtatagat atagaagata
     5461 tgagcagtgt ttttaagatg ttaagtatgt gtgattatgt atctgtagtt gggattcata
     5521 tacatattaa atctcaggta ttggattttg aaaagttagg acattattat gaaaaatgtt
     5581 tttttatagc tttaaattta aataaaagta agtatattaa tattgaattt ataaattttg
     5641 gtagtggact tggagtagct tatgataatc ttagagaaaa tccacttaat cttaaagagc
     5701 tttcaaaatt aactcaaaag attgctttta ctaattcaaa cacactaaaa tcaagacttc
     5761 ttattgaatc aggacgtttc atagtttgta aatcaggtaa ttattatgtt aaaattgtag
     5821 atattaaagt atcaagaggg aaaaaatatc ttattgtaaa aaatggactt aatggattta
     5881 tgagacctat tatttctaat ttaattgaaa atattacttg tggagtagaa ttagaaggtc
     5941 aagagccatt atatacatca aatcatgcct tttctctttc agtattaaat aatttaaaag
     6001 aagaagagta tgtaaatgtt gttgggaatc tctgtacatc attagatgta attgcaaata
     6061 atgttaaatt gaaaaaagca aacataggag atttgatttc aattagtaat gctggaagtt
     6121 actcctattc tttatcaccg ctattatttt caagttatga atttcctaaa caatattata
     6181 aaagaaaaga tggatatatt gagtaaacaa tagcataaat taataaactt agtattattg
     6241 aaaaaattta aaatgcaggg ggtgtaattc tatatggaat tgtggaaaag aaatttattt
     6301 gtatgttgga taggtatgtt ttttagttct atagggatga gccaaatagc acctatactt
     6361 tcattatata ttaaacagtt gggggtaact gatgttagtt taatacaaca gtattcaggt
     6421 atagtttttg gatgtacctt tgtagtagca gcattctttt cgccaatttg agggaaagct
     6481 gctgataaat atggaagaaa atcaatgctt ttacgtgcaa gtattgggat gggaatagtt
     6541 atatttacca tggcgtttgt tcaaaatgtc tatcagttat taggattaag aatacttcaa
     6601 ggagttttta cgggttatgc tactgcatgt actactctta ttgcaacaca gacagataaa
     6661 aatcattcag gatgggcttt gggtactctt gcaactgcat caactactgg ctcattaatt
     6721 ggtccaacag taggaggata tatagaatca atcttaggtc ttaaaagtac atttattata
     6781 acaggaggat tgttgtttgt atcatttatc atctctattt tatttgtagt agataatttt
     6841 aaacctaaag aaataaaaga atcaatttct atacctaaag aacaattaaa tatattgcca
     6901 aataaatttt taattgcatc tatttttgta accacattca ttactcaact agctttatac
     6961 tctatagaac caattgtaac catatatata tcacaactta caaattttgc atcaaatgta
     7021 gctttaatag ctggattgac attttctgca tcaggattag caaatttatt agcagctcaa
     7081 aaacttggaa aaatgtcgga taaaataggt cctcaaaaag tattgttaat ttcactttta
     7141 tgggctggga ttatatttat accacaagct tttgtaaaaa ctgcatggca actgatgttg
     7201 ttgagatttt tattagggtt gtcagttgca gggcttaatc cttctgttaa cagcttgctt
     7261 aaaaaaattg caccagaaga atatgtggga aaaatatttg gatataatgc atctgcccag
     7321 tatataggct gttcatcagg agcattttta gggggacaaa tatcagcaca tttaggtatt
     7381 agaacagtat ttttttcaac aagtttattg ttgtttatga atgcactttg gatgtataaa
     7441 tttactggat tgtttattaa agataagact aaataatcat actatttaca tttctaattt
     7501 tatgatgtaa aataaatatt ttttatgtaa tttatcatag aattatataa aaaataaccc
     7561 tatggtaaaa attggaaaat attattatcg acaaaatctt tgacttaaaa actatattgc
     7621 gatagtatta atttaataaa agtttgagag gggcgtaagg ttatgacaat ttcaaaaata
     7681 ccacaagcag aattaaaagt tatgaagttt atttgggaaa aaaatgatac agtaacatca
     7741 aaagaaacta ttgaagctat ggaacgaaaa tatggttgga aacaaacaac tacattgaca
     7801 cttttatcaa gacttgttaa aaaacatttt ttaagtgcag aaaaaataga tagatacact
     7861 cattatacaa tagttgttgg acataaggag tatttaagtg ttgagacaaa agatttttta
     7921 agcaatatac acgacaattc tttacagagt ttaatttcag cactacatga tgatgaagtt
     7981 gttgataaag atatgttgga tttatttgaa agtcatttta aaaatttaga agaagagtaa
     8041 ttatatcagg atagaactct aagaatttaa ttaatctatg gctgaaaacc ataaatggta
     8101 ttatttaatc agctactcac tattcataaa gtaatattaa ataggttctc taattagtaa
     8161 aatgcctaaa ataattggaa tagggagtat caagttaaat gataccactt atttaaataa
     8221 gaaatgattt ttaaaattta aaataatttt ataacttgtc aatattcatt aatct
//
LOCUS       DAMJZE010000168         8208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJZE010000168 DAMJZE010000000
VERSION     DAMJZE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.172"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            98..1540
                     /locus_tag="PC541_003146"
     CDS             98..1540
                     /locus_tag="PC541_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2840586.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1582..1935
                     /locus_tag="PC541_003147"
     CDS             1582..1935
                     /locus_tag="PC541_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840587.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2126..2947
                     /locus_tag="PC541_003148"
     CDS             2126..2947
                     /locus_tag="PC541_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2840588.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            3205..5088
                     /locus_tag="PC541_003149"
     CDS             3205..5088
                     /locus_tag="PC541_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2840589.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5312..5992
                     /gene="pgmB"
                     /locus_tag="PC541_003150"
     CDS             5312..5992
                     /gene="pgmB"
                     /locus_tag="PC541_003150"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2840590.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            5989..>8208
                     /locus_tag="PC541_003151"
     CDS             5989..>8208
                     /locus_tag="PC541_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2840591.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     "
BASE COUNT         3300 a          776 c         1512 g         2620 t
ORIGIN      
        1 atataattat taaattataa taaatgacta tagcgtagat gtaatactat gttatagtca
       61 tttttgaatt aaacttgata tatcaagggg gaagaatatg agaacaatta gaagagtttt
      121 agcattaggt ttaacattag ctatatttct aataaatgta cctaatgtag atgcattaac
      181 atcagataca attaaaggta acaatatata tgagacggct ggattaatag cagataaaaa
      241 gagttatgat acagcaatta tggtaaatat ggataattct atagcagatg gtctttcagc
      301 aagtggtctt gcaggtgctg ttgatgctcc aattttgctt gcacaaaaaa ataagatacc
      361 aaatgaaaca aaacaaagat taaaaaatgt taaaaagata tacataatag gtaaagaatt
      421 atcaataagt aagtcagtag aaactgaact aaaaaacaca ggggcacagg taactagatt
      481 gggtggagat gacagaataa agactagtta tagtgttgct aaagaggtaa atggtatcaa
      541 aaaagttgat gaagtgatat taactaatgc atataaagga gaagcagata caataagtgc
      601 tgcacccgta tcagtaagag atatagctcc tatagtactt acagatggaa agagtgtgcc
      661 tttttcaaca agcaatgtaa aaacatatgc agtaggtggg agtatatcaa tgagcactag
      721 tttagttaat aaaactaatg ctaaaagact tggtggttca gatagatatg atacaaataa
      781 gaaagttata aaagagtttt atccagatgc atcagaattt tatttgagtg atggatatga
      841 tttagtaaat gcacttacag gttctacaat tgctaaggaa aatccaattg tattggtgtc
      901 agaaagtagt gataagtcta tattagcagg agcagataag attactagat taggttcaat
      961 aagtgacagt gtgtataata agtgtgtttc tgctgcacaa aataatggcg attcgtctac
     1021 aaaaggtgag tcacctatga aaaatgagac aagcatatta ggtcagccaa cagctagttt
     1081 agaagcgtgc ttaaaatggg caaaatctaa aaaagctaat gatttattta tagagttaat
     1141 accaatatta tatgatactg ctgttcagga aggtgttaat cctgttttgg cagtagctca
     1201 atctgcaaaa gaaactggtt tttgtaattt tggtggagta ttagatgcat catttaagaa
     1261 tccttgtgga cttaaaactt ctgtaggtgg ttctgatact gataagaatg cccattcaag
     1321 atttgatact tgggaagaag gaatattagc tcaaattcaa catttatgct tatatgcagg
     1381 acaagatggt tacccacttt caaatccagt agaccctaga catgaaaaat ctttatttgg
     1441 taaggctaaa acagttgaaa gtttatctaa taattgggct ggaggtcaat atggacaaga
     1501 tttagtaaga atgatgggag aaattgaggc aacaaaataa ttgaagatta tcaaatatcc
     1561 aataaataag gattgattat tatgaaaaag agtaatattt ttagtatcat atttcctaca
     1621 gtcataatgg tgctaatgtt tttaactttt gctacagata tatttaatat tccagatatt
     1681 caatctaaaa gtatttttgt tgtgggaatg gttttgattt ttccaatttc ttttttgata
     1741 caagggataa tttgtgtatt aaataagact aattggattt tatctcttat tgtttcttta
     1801 gtaacttata tagtattaat gtatatattt ttaaatgatt ctgcatatat atatgtttta
     1861 ctttatacag ctttttatat gattggacat ataacagcta aactctatta caaaattaag
     1921 acttttaaaa attaaattga agtatataaa agtaacatta ggagaagtat ccttaatgat
     1981 tttctaaaat cagaaatagg tgcttctctt ttttgtaaaa ataaaagtat gtaaataaaa
     2041 tttaaccatt ataattttct tttaacaaat gtatacaata aacttgttgt ataattaaga
     2101 tagataaaat aaagggaggg ataatatgaa tttaatagat aatcatattc atactaattt
     2161 ttcaagtgat ggaaaggatt ccatggaaga tacaataaaa aaagctataa gtattggtgt
     2221 tagatatttg acttttacag accatttaga acatgatgaa gatagaggat tttgcataaa
     2281 ttataatgac tatgttccag tatttaataa gtttaaagaa aaatataaaa aggacataga
     2341 gttattacta ggtgttgaag tgggttacag aaaacatcta aaaaatgaaa tagaagaaat
     2401 aataaattct aatccttttg attttgtatt gtgctcaact catacaattg acaatgtacc
     2461 tgttccatca aaggcgtatt ttaaaggtct tagcaaagaa gatgcgtatt ataaatattt
     2521 taatagcata cttgaaacta atcgtgaatt tggagattac aatatatatg gtcatttgga
     2581 ttatatatct aggtatggaa tatattcaga taatagagtt atatacaatg actttaaaga
     2641 tgttattgat gaggtcttaa agtcaataat caataatggg agtggtatag agctaaatac
     2701 ttctggttat agatatggac taaatgctat acatccaaat gaagatattc taaaaagata
     2761 taaagaactt ggaggattta tagttactgt aggttctgat tctcataggg tagaagatat
     2821 atgcaaagat tttgatgtgg cctatgatat gcttaagtat cttgacttta aatatgtatc
     2881 tttatttaaa gaaagagaaa cctattttgt aaatatagaa aaagttaaat ctaacaatat
     2941 agcttagagt gtgttttgaa gtaaattaat agatagttaa aaagttaata gttatagtat
     3001 tgtatcctct agaaaaatac tagaggtttt tattttgcta aaataactaa atagaattaa
     3061 atttaaatag tatagcttat tttaaattga atagtttatt gttgatatat tttactatta
     3121 tatattttta ttgtgatata atagtaaaat atattaacct atctttagat tatataaatt
     3181 attataaagg agggaaattt taaaatgtct aataaaaata aagaaaatat aactttaagt
     3241 aatcaaatca aaggaacaat tcaaaatagt ttaacaaaag aagctatatt acatatacca
     3301 atgagcaatt atgcctatgg ttatgataga gaaacactac atataagact tagaactaaa
     3361 aaaaatgaag cgaaaagagt aattttacga ataggagacc aatatgtgtg ggataaagga
     3421 ggtgcaggag gaggaaattt aaatgcatct ggagttggtt ggtctggtgg aacaaatata
     3481 gtaatgagta aagaagtaga aactgaatta tttgactatt ggatagctaa gtgtaagcct
     3541 ttaaataaac gctcacgata tggctttata atagaaggtc aagaggaaaa acttttattt
     3601 acagaaaaaa gaataataga gttgggaaat gaaaatgatg aaaaggaatt atgtgaaata
     3661 ggtaattttt ttggatatcc atatttaaat tatatagata tacctaaagt gccaaattgg
     3721 gtgaaagata ctgtttggta tcagatattt ccagatagat ttgcaaatgg tgacccgtct
     3781 ataaacccag aaggagtaga taaatgggga gctattccaa ccagagataa ttttacaggt
     3841 ggagatttac aaggtgtgat agaacactta gattatttga gtgatttggg aataaacgga
     3901 atttactttt gccctattac aattggaaaa accaatcata gatatgatac ggtagattat
     3961 atggaggtag accctacatt aggagataaa gagactttaa aaaaactaat agaagaagcg
     4021 cataagagaa atataaaaat aatgttggat gctgttttta atcatatagg atattattct
     4081 aaacagtggc aagatgtagt tcaaaataag gaaaattctc gatataaaaa ctggttttat
     4141 ataaaagata tgagtaaaat tgatactcca atagaacaaa tagatgaaaa aaatatacct
     4201 tatgaaacct ttgggtgtga aaaatatatg cctaaactta atacagaaaa ttcagaagta
     4261 ataaaatatt tattggatgt aggcaagtac tggatacagg aatttgatat agatgcgtgg
     4321 agattagatg tttcaaatga agtagaccat gttttttgga gaaagtttag agaagaagtc
     4381 aaaaaagtaa aaccagacat ttatatattg ggagaaattt ggcatggtag tttaccatgg
     4441 ttaatgggtg accaatttga ttcagttatg aattacttga tgtcagaagc catgaaaaaa
     4501 ttcttttgta cagatgagat aaacgccgaa gaatttaagt atatgataaa tgatgtaatg
     4561 gtaagttatc caatacaagt tagtgaagtt atttttaatt tacttggaag tcacgatact
     4621 acaagaatat tgacttatgc taatggaaat attgatagat ttaaacttgc atatatgttt
     4681 atgtttgtac aaacaggatg tccttgtata tactatggtg atgaaatcgg aatggaagga
     4741 gaattaagtt caatatctga aggtcaaaga aaatgtatgg aatggaatga agaaaaatgg
     4801 aataaggata tatttgattt tatgaagaag attattagac ttcgtaaaga aaataaagaa
     4861 cttaggtcta tatcaaatga atgggtgtta gcagataagg aaaatggaac gattatattg
     4921 aaaaaagaat ctatttctat aataataaat aattcatcta aaaatataat tttgacgtta
     4981 ccagattatt tagcaaataa aaaagtaaaa gatttatatg aagaagaaat agttgattta
     5041 aaagaagaac taatgttaga gaaatacaag ttcattattt taaaataata caaaaaagta
     5101 tttattttag atttcatgtt aaattattat acagtttaaa caagtcttta taaacgataa
     5161 aaaatatggt ctacaagtga gtgaattgaa agtagatttt aaattaaaga ctgagttaga
     5221 gtagataatt ttataaataa tatcttaaat acaagataga aaataaattg attattagtc
     5281 taaaaatttc ttgtagaaag aggaaataag aatgattgaa gcatttatat ttgatttaga
     5341 tggtgtaatt acagatactg catattatca ctatatggct tggagaaaac tagctcataa
     5401 agttgggata gatatagaca caaaatttaa tgaatcttta aaaggcataa gtagaatgga
     5461 gtcattggac aggatattag aatttggaaa taagaaatat tcattttcag aagaagaaaa
     5521 agtgagaatg gctgaagaaa aaaataatta ctatgtatca ttgatagatg agataacttc
     5581 aaatgatatt ttgccaggca tagaaagctt gttaattgat gtaaaatcta ataatataaa
     5641 gataggtcta tcttctgcaa gtaagaatgc aataaatgta ttaaatcatt tgggtataag
     5701 tgacaaattt gattttatag ctgatgcagg aaaatgtaaa aacaataaac cacatccaga
     5761 gatatttctt atgtcagcga aagggcttaa tgtaaatcca caaaattgca tagggataga
     5821 ggatgcaagt gcaggtatag acgctataaa ctcagctaat atgttttctg ttggtgttgg
     5881 aaattatgaa aacttaaaaa aagcaaattt agttgtagat tcaacaaatc aattaaagtt
     5941 tgaatatata caagaaaagt ataatgagta tatagttagg agaattatat gaaatgttta
     6001 tttagtaatg tagatgagtg gaagattata caagataaga ttgaaatcaa agaaaataga
     6061 ttagcagagt ccataatgag tataggcaat ggatatatgg gtatgagagg aaactatgag
     6121 gaaaattata gtggagatac acacaagggg tcttatatag ctggagtatg gtttccagat
     6181 aaaactcgtg ttggttggtg gaaaaatggg tatccagaat actttggtaa aattcctaat
     6241 tcagtcaact atataggtgt aagaattttc atagatgatg tagaattaga tttagcaaaa
     6301 tgtaaagtag aagattttca tagagaatta gatatgaaaa atggtatttt aaatcgtagg
     6361 tttattgtaa atataaatga taaaaaattt gaagttaggg ttactaggtt tttaagtata
     6421 tctgttaagg aacttgcagt aattaaatac agtataaaat cattaaattc taatactaaa
     6481 ataaaatttg taccatattt agattcaaat ataaaaaatg aagattcaaa ctatgaggaa
     6541 aagttttgga atggaatttc ttcaagttct ttagaatata aagggagtat tctttctcga
     6601 acaaaggaga atgcatttaa tacaccagta ttcactgtgg gttcaatgat gtatattaag
     6661 acaaatggtg atgtagaagc tgtatctcat gagagtgatt ataattactg tgaaaattcg
     6721 ataacaatgg atgttttaaa aaatgaggtt gcaagtatag aaaaatatgt atctataacc
     6781 tcaactagag attataagga aagtgagcta ttaagaaaat gtgaagacat tttaaataga
     6841 gaactatcta aaagttatga tgatatatta aatgaacata taaagttatg gaataaaaga
     6901 tgggaaagtg cagatgtaaa aataaatgga gataaaagta gccaacaagg tataagatat
     6961 aatttattcc aactattttc aacatattat ggagaagatt cgcgattaaa tataggtcca
     7021 aaaggattta ctggtgaaaa gtatggagga gctacttatt gggatacaga agcgttttgt
     7081 gttccagtat atctaggtgt agcagataag gaggtaacta gaaatctttt agaatatagg
     7141 tacaatcagt tagaacaggc aaaggataat gcaaaaaaac taggtcttaa aggtgcttta
     7201 tatccaatgg taacatttga tggagtagaa tgtcataatg aatgggagat aacatttgag
     7261 gaaatccacc gaaatggaag tatagtatat gcaatatata actatacaaa ttatacaggt
     7321 gactataaat atataaaaga aaaaggaata gatgtaataa tagaaatagc tagattttgg
     7381 gcaagtagag ttcatttgtc tacaaaaaaa gatttgtata tgatacatgg agtaacaggt
     7441 ccaaacgagt atgaaaacaa tatcaataat aattggtata ctaattatat agctaaatgg
     7501 tgtttagatt atgctgtaga aaatatctta cgattagaga aagagtgtaa tgaatgtatt
     7561 gacagaaata atgtgactag ggaagaagtt gagttttgga aaataatatc aaataaaatg
     7621 tatctgctgt atgatgagga actacaaata ataattcaaa atgataattt tttagataaa
     7681 gaatttgtag aagtaaagga tttaccaaaa gaaaattttc ctcttaatca aaattggtct
     7741 tgggataaga ttcttaggtc gtgttttata aaacaagcag atgtattaca gggaatttac
     7801 tattttggaa atagatttac taaagaagaa aaaaagagaa attttgactt ctatgaaaaa
     7861 tatactgtac atgaatcttc tttatcttct agtatttatt ctattattgc ctcagaaata
     7921 gataacctag aaaaggctta tgaattatat tcaagaacag ctaggcttga tttagataat
     7981 tacaataatg atacaaatga tggactgcac ataacttcaa tgtctggttc atggctttcc
     8041 attgtacatg gatttgcagg catgagaaca tggaatgaaa ctctatcttt tgaccctaag
     8101 ttgccaaaag agtggaaaaa ttattctttc aatataaatt atagagataa tattattaat
     8161 gtagatgtat gtagtgattt aattaaaata gaaaatttaa aaggtaaa
//
LOCUS       DAMJZE010000169         8147 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJZE010000169 DAMJZE010000000
VERSION     DAMJZE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.173"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..597
                     /locus_tag="PC541_003152"
     CDS             <1..597
                     /locus_tag="PC541_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2840592.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="PC541_003153"
     CDS             728..1750
                     /locus_tag="PC541_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2840593.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="PC541_003154"
     CDS             complement(1914..2471)
                     /locus_tag="PC541_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2840594.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="PC541_003155"
     CDS             complement(2477..3553)
                     /locus_tag="PC541_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2840595.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC541_003156"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC541_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2840596.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC541_003157"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC541_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2840597.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="PC541_003158"
     CDS             5391..6320
                     /locus_tag="PC541_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840598.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="PC541_003159"
     CDS             complement(6481..6930)
                     /locus_tag="PC541_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840599.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="PC541_003160"
     CDS             complement(7133..8113)
                     /locus_tag="PC541_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2840600.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2815 a         1347 c          979 g         3006 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgtttgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttataggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaat ccccttgctt tcttatatat atttggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaatg cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaagtcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgcc cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acgttataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtctatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac tgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatatt
//
LOCUS       DAMJZE010000170         8096 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJZE010000170 DAMJZE010000000
VERSION     DAMJZE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.174"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(140..967)
                     /locus_tag="PC541_003161"
     CDS             complement(140..967)
                     /locus_tag="PC541_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2840601.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(1015..2451)
                     /locus_tag="PC541_003162"
     CDS             complement(1015..2451)
                     /locus_tag="PC541_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2840602.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2538..3407)
                     /locus_tag="PC541_003163"
     CDS             complement(2538..3407)
                     /locus_tag="PC541_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2840603.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3565..4218)
                     /gene="phoU"
                     /locus_tag="PC541_003164"
     CDS             complement(3565..4218)
                     /gene="phoU"
                     /locus_tag="PC541_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2840604.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4246..5010)
                     /gene="pstB"
                     /locus_tag="PC541_003165"
     CDS             complement(4246..5010)
                     /gene="pstB"
                     /locus_tag="PC541_003165"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2840605.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(5029..5853)
                     /gene="pstA"
                     /locus_tag="PC541_003166"
     CDS             complement(5029..5853)
                     /gene="pstA"
                     /locus_tag="PC541_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2840606.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5855..6772)
                     /gene="pstC"
                     /locus_tag="PC541_003167"
     CDS             complement(5855..6772)
                     /gene="pstC"
                     /locus_tag="PC541_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2840607.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7153..7599)
                     /locus_tag="PC541_003168"
     CDS             complement(7153..7599)
                     /locus_tag="PC541_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840608.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
BASE COUNT         2594 a         1475 c          853 g         3174 t
ORIGIN      
        1 caagattatt ttattttaat tttaatgctt tctcattaat tttatattat ccttacagta
       61 tacctcttat tcgtattttt tatattatat gttttatttt ataaataatt tactttatct
      121 tttactatat ttaaatttat tataagtttt cttgccagaa atttttaggt gttatatttt
      181 ctgtaattgg caatattgta tatcctctct cttttaagat tcttataata ttatttaaag
      241 catttaaact ttgctctttc tcatgaatta atactactaa ttcatctctt tttcttccat
      301 aatataatat attacttact atctgacttg tagaagactt ccaatcctgt gtatccaaat
      361 tccagtccca aattaaataa tcatttgcca taagtttatt atatgaacct tctggcatat
      421 atggtttact tccgtatggt aatcttatta attttgaact ctgaccagtt atttcttgta
      481 gagtattatt gcatgtatta aattccccaa ctgttacttc tgggctttta tataacttat
      541 gtatatcatg acttacacta tgaaaaccta ttgagttttc ttcttccaag actctcctta
      601 cttcttcctt gtggtttttc atattctgat taagcatgaa aaatgtcgct ttgacatcat
      661 tttcttttaa aatatcaagt agtgcatttg tatattttga tggaccatca tctattgtta
      721 tgtatgctac tttttcagaa gtagcccctg gaccttttgt ttcttcttga gatgctttgt
      781 atatgatttc tcctaaagta ttactttgag aaagcagttc cagtgtattt tcaccttttg
      841 aacttatatt ttcaactgga acattattaa ttttttcctc caataatgtt ccagttaaaa
      901 ttaaaacagc tataagaaat atccttataa atacatgaaa aacagcctta ttatctttaa
      961 ttttcatccc ccatatctcc cttttataaa agcataagtt taagacttaa aaagttattt
     1021 tttatattta ttctctttct tattactttt tatatctcta ctaaatattt ttttaacctt
     1081 ttcttcttct ttttctccta aagattctac tcttttgatt cccaaagaag ttaaaaattg
     1141 taatacattt aaattatcaa atggagtttc catattcgta ctttcactat attcatcaac
     1201 attcttagta ccttcttcca cacttaaaat acgcttcgga cctcctacac agcctcctac
     1261 acaccccatt ccttctataa atgtagcttc tatttcttta tttagaactt tctttagacc
     1321 gtccatgcac tcttttgttc cttcaaatgc cttttcttca aattttatat ttttgtcaat
     1381 tcttctaact gataacttaa ctgctttact tacacctcct gttctagcat aaactctacc
     1441 tgaaagggac gcctctactc tattactctc atccattatt gctggattta actctaatgc
     1501 atcaaatatt tcttgcaact ctttaaatgt aagcacaaaa tcaactgcat cttttatatc
     1561 ttctaacatt gcctctttct tttttgccat acaaggacct atgaatacaa ctttagcatc
     1621 tggatttaaa atttttacag ctctcccaca agcaatcata ggagaaacgg atggtgatac
     1681 attctccaat atttgaggaa aactattttg tatcaaacta acccaaatag gacagcaaca
     1741 actagttata aagcatcctt cttcgtcctt tttcatatgc tcataataat catatgcttc
     1801 ttttgcagta agcatatctg ctgccaaagc aacttctatc atatcttcaa atcctatctt
     1861 ttttaatgca gttcttaatt taccagggct tacatctttc ccaaactgtc ctacaaaagc
     1921 tggtgcaact attgcatata cagggctctt tatatctttt aacaatttta tcattggtat
     1981 aaattctatt ttatctgaaa ttgcacccaa cttacatctt gatacacaat caccacaagc
     2041 actacataca tcttcctcat tacaattaat atgcttactt cttatcttac atgactcatc
     2101 ttccttatta ttttcatcac aatatttgca agcatctgat attacagata ttattggtct
     2161 tttgacttca tctaaattta cagcttcatg tagtgactct tttaaatttt tatctgtatc
     2221 ttctgtcaca tccaatccca tagttaccct aattaaattt accatagtat ctttatcaaa
     2281 ttttccacta aaataattat ttaaattatc ctttaaattt tcatttaaaa ttttattagg
     2341 taagttttca atatcctcta tgtcatcatt ccacactgct tttacaagtt cgccaaaaag
     2401 cttcatttta tatatttcta gtattttaga attgtcattt ctagtattca taaaaaatag
     2461 tacctccata aattaaactt atatatgttt aatatttaca aagatactat tttttattta
     2521 ttatttaact actattatta atatgattta tttagttttc tcaacaactc tatttcaatc
     2581 attattttca ttagatgata gttgaataca gggctttttt cttcatctat attccagtct
     2641 agcttatcca cattatatgt tttcttaagg tatttatatg tatgctcttt caaataaagt
     2701 ttattttcaa gtaactctat agactgcata tgaaaatgta tttctctaca tatccttaag
     2761 gcattctcaa ttttttctat attagtttca ctcaaactat aatccaactc atcaataagt
     2821 tttgaatata tttcttctaa tttttctact tcttcttcaa atttctctac atttaaattt
     2881 tcttttctaa gaattttatc ttcaataagc aacattgaaa gttcttctat ctttttaaac
     2941 tcatctataa cactatttag ataatttggt ctagcaacat aataattaac aagtactcca
     3001 aacaaaacac ccacagatgt atctaacacc ctatgtaaag aataatacac agggtcacta
     3061 tttcctacac ctagatgtat agccagaaag gttacacagg caactactac tgactttatt
     3121 ttaaacagat tgcataaata tattgagcac attactccta gaccacataa aaatgggtct
     3181 ccagctttaa tgatggcaaa tatgaatcct attataccac ctaaaatagt accttccact
     3241 ctattgattc ccgtctttaa agaacctttt actgtatctt gaacagaaat tatacatgct
     3301 accagagaat aaaacatatt ctctactaaa tagtcaccaa gaatcataca taaagtgaca
     3361 ctaattcctg tttttatagt cctcataccc atcttttgaa gtttcaaagt tccacctcct
     3421 aaaaagcacc ttatataaaa tacgcatcct catatttaat atgttcctag tatatcacaa
     3481 ttttttaaat ttacccatta atcatatttt tttatcaatt ctttcttaat ttagacaata
     3541 aaaaaactgc tttaaagcag ttttttatcc tatctcaacc attttaccct caattgcaaa
     3601 aattatcttc tcacatacat ttgttatatg gtctccaatt ctttcaaggt atcttcctat
     3661 aagtagaagt tttacccctt ggtctatggt atttgagttt tcatgcatta tttttaaaca
     3721 atcaatttgt acttgttcat acaatccatc tacaatatca tctttagttc caacatcata
     3781 agctatactt gcatcaaata agattaaagc ttctctagtc tgagacatca tagacttaca
     3841 ttcttttgcc atcttaggaa tatctattag ctcttttata taagcctctc cacctatctt
     3901 caacatttct ctagctatat ttgctgcata atctcctatt ctttccagct ctatagatat
     3961 attacctaat gaataaataa atcttaagtc ccttgccatt ggttgcttta atactattag
     4021 ctctatactc ttatctctta tttcttctct taatttattt atttcttcat catcttctac
     4081 tacttttctt gcaccctcta aatccttctc tatcatacaa gtcacagact tttctattgc
     4141 ttcttcacat ttttccatca taagcactgt ttgttctttt aaagtatcaa tacttatatc
     4201 taagctatta ttgaccattg tctaactcct ttatagttat atattttaac caaatctacc
     4261 tgttatatag tcttctgttc ttttatctac aggagtggta aacatagttt ttgtatcgct
     4321 aaattctata acctctccat ttaagaaaaa agctgtttca tctgatatac gagcagcttg
     4381 ttgcatatta tgtgtaacta tgactattgt gtagtctttt tttaaatcct ctataagttc
     4441 ctctactttt aatgttgaaa ttgggtctaa agcagatgta ggttcatcca taagaataac
     4501 ttctggtctc atagcaattg ctcttgctat acatatacgt tgttgttgac caccagaaag
     4561 tcctagtgca gatgatttaa gcctgtcttt aacctcatcc caaatagctg caccttttaa
     4621 gctttcttct acaattttat ctaattgctt cttatctctt agtccatgtg tacgaggacc
     4681 atatgccaca ttatcatata tactcatagg aaatggattt ggcttttgaa ataccattcc
     4741 tactttagta cgaagattta taacatcatc tgaggtatat atgtcctctc catcaacaga
     4801 aatgtttcct ttaattgtta catcttcgat taagtcattc attctattta acgttcttat
     4861 aaaagttgat tttccgcatc cagatggacc tattaaagca gtaaccttat tctcttttat
     4921 atccatattt attttcttta atgcttgctt atcaccataa aataaatcca aatcttttac
     4981 actcattttt atcttatcga ttaattccat tattcacacc tcttaaattt aataattagc
     5041 tttatttaat ttcttagcaa caaatcttgc tagcatgttt agtgctaata caattactat
     5101 taaaacaatt cctatactgg ctgctgttgc tatatctcca ctctctttag tcagtaagta
     5161 tgagtgtaca gttaatgttc ttgcactatc aaatattcca cctggcatct tggctacagt
     5221 acctgctgta agtaatattg cagctgattc tccaattata cgaccaactg ataagattac
     5281 tccagataaa attcctggta ttgcacttgg aagtactacc ttatataaag tttgaaattt
     5341 tgtagctcct aatgccaatg atgattctct ataagcttga ggaactgttt ttaaagcttc
     5401 ttctgttgta cgaattatta ctggtaatat tattattgca actgtaagtg aacctgcaac
     5461 tatggaatat cccattttta atgtaactac aaagaaaatt cctccaaata atccatatac
     5521 tatagatggt atccctgata aactttcagt tgcaaatctt atagctgtaa ctgcttttcc
     5581 ttttttagca tattcttgta aatatatagc agccaatatt cctataggag ttgctacaac
     5641 tatagaaact actactgtat ataatgttgt aattatcatt ggaagtatac caccatcacc
     5701 tgatgctgaa taattgctaa ataagaaatt aaaattcatg ttgccaattc cctttataaa
     5761 tataaatccc actataacta ctaatgatac tattgtaaat agtgctgata gatatactaa
     5821 tgacttcaat agattctctt tgaactttct catcttaatt taccgcctta tttgaaagtt
     5881 tatttaaaac taagtttaaa attaatataa atgtaaacaa tactactcca gttgcaaata
     5941 gcatttcttg atgtgtacca aatgcgtatc ccatttcaag tgctatatta gttgtaagtg
     6001 gtcttacact atctgttatt gcagttggca taactggtga gttgcctgca acaagtatta
     6061 ctgccattgt ttcacctaaa gctcttccta ctcctaacac tactgcggct aatactccag
     6121 attttgcagc aggtaataca actttgaata ttgtttctat ttttgatgca ccaagtgcta
     6181 atgaaccttc tttgtatgct tttggtactg ctcttattgc tgtttctgat acagatatta
     6241 ttgttggtaa catcattact gctaatacta ttactattgc taacaaactt tgaccttttg
     6301 gaagattaaa tatactttgt atagttggaa ctatcactgc aagaccaaat acaccatata
     6361 atactgaagg tatacctgct aaaagttcaa ctgctggtga cattattttt gctaatcttt
     6421 taggtgctac ttctgctata aatacagagg ttaatatacc aacaggtact cctataacaa
     6481 gtgctccaag tgttgcaaat attgatgcta ctatcataga tgatattcca aatttattgg
     6541 cagaaggaac ccaatctgca ccagttaaaa agtctataaa tgaatatcct tcaaatataa
     6601 atggtcttaa tcctttataa aatacaaatc ctattatcaa aagtaaactt gcaactgcta
     6661 ctaaagcact aagtagaaaa atattttttg ctactttttc aattacgtat tttgtcttat
     6721 tgccattatt gtcatttttt tctaaaccta ctaatctaga ttttactatc ataataccct
     6781 cccattaaaa tatacttatc gattttcgtt ctgtattcaa aatttaattt tttataattc
     6841 gtcttcatca aatttcctgt acttttattc tacttttttt aagttaaatc tatattagga
     6901 ttaagttaag caagtgtaaa attatattta ctttttaaca ttaattttac ttttaaaact
     6961 atatttgaca tttactttac aatatactgt tgtttctcta tattaaaata gcagatatag
     7021 ataataatac agtttatgta taatttatta cctatatctg ctattaagat ttgttaacta
     7081 aatttaactt ttatactaca tatataatta ttgtatcctc tagtttatat cactattatt
     7141 tccatcaaat tgttacgata cttgatgttt gcaataggta tgaatagcca aaaatataaa
     7201 aataggtatt tctatcactg agaaaaacag tataatgtgt ggtgtccaaa aactcatacc
     7261 tcctaagtgt aagaaattaa atgatgtcat ttgatataat aaactaatct taaaaccaat
     7321 tccccctggc ggtaataggt atccaatcca actaacacct attcccatag gaattaagca
     7381 aatagaaaaa gcaatcagca tagaagtaag agaatcctta cattttgcag ataaaaataa
     7441 tgttaaacta accattgcaa gtattgaaat tagaccacca atagctataa gaatctgtgc
     7501 ttgtagtaaa tttatattta ctagactaat gacagaaaac aacatttgaa atgaggtttt
     7561 caaacattca gtaccaaaag ctaaatcaga aattgccaaa tagatagaaa tacatataac
     7621 aaacattatt ataaatatag aaaatagagc taatatttta gtaaacgcta gacaaatatg
     7681 tccatgtttc gtacaacgaa ggatgctatc tgattgtgtc tgatactcac cagaaaaagt
     7741 aggtgcagca attgcaacat atataattaa taatataaaa atataaaata ttatataatc
     7801 gaaagcatct tctggattta ttaagaaatt cttaatctta ttatttaact attttattat
     7861 ttaatatata ttaaaggtaa aaaaataata tatagattta gatatacaat ttatctatct
     7921 tttcaaatat aatctataaa ataatagttc cattacgcat ttttttatat aataaaactg
     7981 actgtaattt aactggcaaa gtctagaaga ataggcttta tcaattaaat tacagtcagc
     8041 tattttataa taatttagtt tagatattgt tagtatttca catttataac aattat
//
LOCUS       DAMJZE010000171         8090 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJZE010000171 DAMJZE010000000
VERSION     DAMJZE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.175"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            108..569
                     /locus_tag="PC541_003169"
     CDS             108..569
                     /locus_tag="PC541_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2840609.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            637..2883
                     /locus_tag="PC541_003170"
     CDS             637..2883
                     /locus_tag="PC541_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840610.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            2876..4708
                     /locus_tag="PC541_003171"
     CDS             2876..4708
                     /locus_tag="PC541_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840611.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            5427..6704
                     /gene="hflX"
                     /locus_tag="PC541_003172"
     CDS             5427..6704
                     /gene="hflX"
                     /locus_tag="PC541_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2840612.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            6937..7434
                     /locus_tag="PC541_003173"
     CDS             6937..7434
                     /locus_tag="PC541_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2840613.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            7484..8008
                     /locus_tag="PC541_003174"
     CDS             7484..8008
                     /locus_tag="PC541_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2840614.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         3203 a          834 c         1473 g         2580 t
ORIGIN      
        1 taaatgaaat ttatatgaat tttttataaa tatattgaaa aaatgcatgc agtatagtaa
       61 aataatgctt agccgactaa gtaatataaa aaataaggag ggcaagtatg aatagcaatt
      121 ctgaagaaaa attaaatgat gttttcttgc aattaatgcg aatatatttt ataagaactc
      181 atttttattt tgataaatta ggcttatatc atggtcaacc ccaaatactt ttttctttaa
      241 agatgaaaga tgggcaaagt caaaaagaat tagcagataa aaggaaagta aaagcatcta
      301 caactacagt tatgataaag aggatggaaa aagctggact tgtggaaaga aaacaagatg
      361 aaaaggacca aagagtatct aggatttttt tgacagacaa aggtttagaa atatgtaata
      421 aagttgaaga atttaacaag gaattggaaa gagagttttt tttaggattt agtgaagaag
      481 aaaaaatagt acttagaaga cttctaatgc aagtacgaga taatttaaaa gcaggatgtc
      541 ataagcatga agatgaaaaa tatttttaaa gtttaaaata atgtttttca atttaatgtt
      601 attagactaa ttaatattac agaagggaag aaatgtatgt ttaaattaat taaatattta
      661 aagaaatcgg cattatcaat agtaataata gtctgtctat tggtaattca agcggtatgt
      721 gacctttctt taccagaata cacatccaat atagtaaatg taggtataca acaaggtgga
      781 gtagaaaact ctgttccatc tgtgattaga gagagtgaat taaataaaat tactttattt
      841 atggataaaa gtagtaagga taaagtacta gataattata ctttgctgaa taaaaaggat
      901 tatgttaagt ataaagataa gtatccaggt ctaaaagatg aatctcttta tgagttaaat
      961 actaaagaca aagacactat agatgattta aatgttattt ttggtaaagc tattttaata
     1021 gtatcagggc ttgagggcga tagtaaagag gtaaaagcta tgaaagctca gttaatgagc
     1081 aaacttccac cacaagctac tcaaagtgga gatgttgata tatttaaatt gttatcagca
     1141 atgccaaagg aacagttaga tacattgact aaagagatga gcaaaggctt tgaaagtatg
     1201 ccagagagta tgataacaca aagctctgtt tcatatgtaa ggtcagaata tgagaaaata
     1261 ggtattgata cagaaaaaac tcaaaataat tatatattat ttactggtgc taaaatgctt
     1321 ggtatagcaa tgattagtat ggtagctaca ataacagtag gatttttagc agcaagagta
     1381 gccgcaagtg taggtagaaa tttaagaagt ggagtattta gaaaagttat gtcattttca
     1441 aatactgaaa tgaatgaatt ttctacagct tcattaatta ctcgtagtac aaatgatatt
     1501 caacagatac aaatgttgat ggttatgttg cttagaatag tattctatgc acctataatg
     1561 gctataggtg gagtgataaa ggttttaaac acaaatactt caatggcctg gattatagca
     1621 gtagcagtag ttgctatctt atcattaatt gtagtgttat tctcagtggt tatgcctaag
     1681 tttaaactgg ttcaaaagct tgttgataag ttaaacttag taagccgtga aataattact
     1741 ggtattccag ttatcagagc ttttagtact gaaaaacatg aagaagaaag atttgataaa
     1801 gcaaataaaa atttaactaa aacaaatctt tttgtaaata gagttatgac atgtatgatg
     1861 cctgctatga tgcttataat gaaccttatc acagttctta tagtttggag aggtgcatac
     1921 agtgttgatt ctggagcaat gcaagttgga gatatgatgg catttataca atatacaatg
     1981 caaataataa tgtcattctt aatgatatca atggtatcta ttatgcttcc aagagcatcc
     2041 gtttcagcta cacgtataga tgaagtatta gtgtcagata taagcataaa agacccagaa
     2101 aaatcagagg cacaagagtt taaggaagat aaaaagggtc ttgttgaatt taaaaatgtt
     2161 tcgttcatgt atccagacgc agaagaacct atattaacaa atatatcttt tacagcaaag
     2221 ccaggagaaa caactgcctt tataggaagt actggtagtg gtaagtcaac tcttataaat
     2281 ttaattcctc gtttctttga tgttactgaa ggagaaatat tacttggtgg agttaatata
     2341 aaaaatgttt ctcaacatga tttaagggaa aaaattggat atgtacctca aaagggagta
     2401 ttgttttctg gtactataga atccaactta aaatatggta gaaaagacgc aacagatgaa
     2461 gaaatgagaa cagcagctga aatagctcaa gctacagaat ttatagattc taaaccggaa
     2521 gcttttaaaa ctgaaatttc tcaaggtgga acaaatgtat ctggaggtca aaaacaaaga
     2581 ctttctattg caagagcaat agcaaaaaat ccagaagttt atatatttga tgatagtttt
     2641 tcagcacttg acttaaaaac tgatgcagct cttcgtaagg ctttaaaatc aaaaactgct
     2701 gaaagtacag ttctaatagt tgcacaaaga attagtacta tcttaaatgc agaacagata
     2761 attgttttaa atgagggcga aatagttgga ataggaacac ataaagagct tctaaaaaat
     2821 tgcgaagttt atgagcaaat agctttatca caactttcaa aggaggagct agctaatgag
     2881 tagaatggga agaggtccta tgggaaaatc tatgggagca ggacaaaaag caaatgactt
     2941 taaagggact atgcgaaaac taattgcata tttatctaaa tttaaaatat caataattct
     3001 agtaattgta tttgctattg gtagtgcgtc gttttctata gtaggtccaa aaattttagg
     3061 aaaggcaact acaaagatat ttgaagggct agtgagtaaa gtttctggag gaaacgtagg
     3121 tatagatttt aatgcaattg gaaaaatctt aaccttttta cttttccttt atttgataag
     3181 tgcactgttc tcttttatac aaggatttat aatgagtgga atttctcaaa aagtatccta
     3241 taatttaaga aaagagattt cagcaaaatt ggatagattg ccaatgaagt attttgatac
     3301 taagacgcat ggtgaaatat tatcgagaat tacgaatgat atagatactt taaatcaaag
     3361 tttaaatcaa agtatgacac agcttataac atcagtaaca actatgatag gtgttttgat
     3421 aatgatgtta tctataagtg gaattatgac attagtggca gttttaatat taccaatatc
     3481 aatgtttgta atttcaagaa taataaagaa atcacaaaaa tactttagat atcaacaaga
     3541 atatttagga aatgtaaatg gtcaagttga ggaaacttac agtggccaaa caatagtaaa
     3601 agcatttaat cgtgaggatg aagtaattga agagtttgat aaactaaatg atagcctgta
     3661 taattcagcg tggaaatctc aatttttatc aggtataatg caaccactta tgatgtttat
     3721 tggaaacttg ggatatgtaa tggtatcaat cttaggaggt tggcttgcaa ttaagaaaac
     3781 aattgaggtt ggtgacatac agtcatttat acaatatgta agaaacttta cacagccaat
     3841 gactcagatt gcacaggttg ccaatttatt gcaatcaaca gcagcagctt ctgaaagagt
     3901 atttgagttt ttagaagagg aagaagaagt ccaaattgtt gaaaatgcag tttcaataga
     3961 tggtttagaa ggtaaaattg attttgaaaa tgtcaacttt ggatataacc caaacaagac
     4021 tataataaat gatttctcag taaatgttaa acctggtcaa aaagttgcta ttgttggacc
     4081 tacaggagca ggtaaaacta caatagttaa actgttaatg agattttatg atgttaatag
     4141 cggttcaata ctgattgatg gtcataatat taaagatttt aatcgtagtg agcttagaga
     4201 aatgtttggt atggttcttc aagatacatg gttatttagt ggttcaatta tggaaaatat
     4261 aagatatggt aagttgaatg ctacagatga agaagttata gaggctgcta aatctgcaca
     4321 tgttcataga tttattaaga cattaccaga tggatataag atgaaattaa atgaagaagc
     4381 aagtaatgtt tctcaaggtc aaaaacaatt attgactata gctcgtgcta ttttagctga
     4441 tcctaaaatt ttaatattag atgaagctac aagttctgtc gatactagaa cagaagtatt
     4501 gattcaaaaa gctatggata atcttatgga aggtagaaca agttttgtta tagcccacag
     4561 attatcaact atacgtgatg ctgatatgat tcttgtaatg aatgaaggag atatagttga
     4621 acaaggaaat cacgaggaac ttctaaagaa aggcggattc tatgccaatc tttacaattc
     4681 tcaatttgaa gaagatgaag caatgtaaca aaaaactatc ttagcataga catgtaatta
     4741 ataagataaa ataaatgagg gtgccttaaa atgaactttt taattcataa ggcactcttt
     4801 tgtgtatgaa ataaagtgta tttttatcat tttaacaatg aaaaagagga ttctctatat
     4861 tttgagatag ccacataatt ttatgtcttt acaaagtgtt tattttatta taatatgtag
     4921 taaggaaaat gttggaggtg attatcaaaa tgtatattta attttattct ttaaatttta
     4981 ttataaatag aatttaattt taatagttga tgattatatt gaagtatatg tttttattac
     5041 ttttattaat catgcaaata actacttaaa tttaagaata gggttaaata taaaatgata
     5101 attacatata agttaattaa actaatgtat ttttaatata tatttagaat tttaatcctt
     5161 cttatgatat aagtagcttg ttttgtatta cttagatttg taaggagttt ttttattgaa
     5221 aaaactgcat aatatatttg ggaggaggtg taagaaagtg attttttata taagattgag
     5281 aaaataatgc acgattatgg ttattataat agtaaataag aagtgtcatt taaggataag
     5341 ataaaaagca aaattcttga tgaatttaat tatagagcca taatctcgtg ctatagatta
     5401 aataaaaaat agcagataag gagaatatgg atatggcgaa aaaaggaata acagtaggat
     5461 taaacataaa taataaatct gaggacttta atgaactaat gattgaactt gaaaacttat
     5521 gttcagcatg tgatattgat gttgtaggga gtataactca aaatgcaaag caggttaata
     5581 gagcatttta cattgggaca ggtaaagttg aggaaatatt aaacttaata aagaaagaaa
     5641 atatagagat agttatattt tacaatgaac tttctacatc acaactaaaa aatcttgaag
     5701 aaaagcttaa ttgtgaaatt atagatagaa cggcattgat acttgatata tttgcacaaa
     5761 gggcaaaaac tagagaagct aaacttcaag tagaagtagc aagtttaaaa tatatgttac
     5821 caagacttat aggttcaaat gaaaatttag gacgtcaaag tggtggagtt ggtactaaaa
     5881 atagaggttc tggtgagaaa aaattagaac ttgaccgtag aagaatagaa gaaaagataa
     5941 catctttaaa tagggagttg gatgatttaa aattccaaag agaaactcaa agaagtatga
     6001 gaagaaaatc aaatcttcca aatgtagctc tagtcgggta tacaaatgct ggtaaatctt
     6061 caataatgaa taaactagta gatatattta aaaactctga agaaaagaaa gtgtttgaga
     6121 aaaacatgtt atttgctaca cttgaaactt ctgtcagaaa tatagttctt gcaaataaca
     6181 aagaattttt attatctgat actgttggtt ttgtaagtaa tctaccacat gacttagtaa
     6241 aagcatttag gtcaacatta gaagaagcat gtgaggcaga cgtactgtta catgttattg
     6301 atatttcaaa tccaagctat aaaagtcata ttaaagtgac agaagatact ttaaaacaaa
     6361 taggtgcaga tggtatacct atgatacatg tgtataataa aattgattta atagatgtag
     6421 aagtattgga tagaatttta gatagtatcg ataaagaagg tatatttgtt tcagttaaaa
     6481 aagatataaa tatagataaa atgataaaat gtatttgtga tagcatattt aaagattatg
     6541 taagatgtaa gttcttaatt ccatatgata aaggtcatgt agtttcttat tttaacgaaa
     6601 atacgagcat tataaataca gaatacagag aagatggagc tattttagat gtagaatgta
     6661 gcaatataga atataataag tataaaaagt atgctttaga gtaaattttg aaaaacttat
     6721 tatagcctaa aatatattta atttataatt tattataaat tgattgtaaa tatgatttta
     6781 tgaaacggta tagccataat gctatgccgt tttgccgttt ataataaaaa taaatttatt
     6841 gacataatag taactaatta ctataatata aagtaattag ttactattta aaattatcaa
     6901 aaaattaaaa tctaagataa agagaagtgt ataataatgg atgcagaaag tttaatgaaa
     6961 aaatatatgg tagatgatga tagtagaaaa caagctatat ttgcaagtat atttgtaatg
     7021 caaaataaac ttcaaactac atgtgataaa ttagacccag aattaacaat gaaacaatgg
     7081 ttacttttag ctattgcgga ctctgtagat gaaactctta cattaaccaa acttggagaa
     7141 ttaatggggt gctcaagaca aaatgtaaaa aaactagcta ttgctttaga aaagaaaggt
     7201 tttatacaaa ttgagcagaa tgaaaaagat tcaagagcta tctgtcttgt gacaaaagat
     7261 agagtgcaag agtattctaa aaaagtaaga ggtatgcagg aaaaagtatt acagctttta
     7321 tttgaagatt ttacagagaa agaagtggaa cagttatttt ttggaattat gaaattaagt
     7381 ataggaattg aaaatgtgaa aaattatgta gaaaataatg acgttggaga ataagcaagt
     7441 atcaaatcaa ctttgtatga catcagagag ggggataatt tatatgaaaa atgtagtagt
     7501 gatttataag agtaagtatg gttcaacaaa aaaatatgct aaatggcttt cagaattatt
     7561 gtcttgtgat atttttgaaa ctaagaatat taatatagaa aaggttatga agtatgatac
     7621 gattatttta ggaggaggtc tttatgcttg taatatagcg ggaatttctt tcttaaaaaa
     7681 aaattataaa gaattgcaga aaaaaaattt agtagtattt ggcgtaggag ctgctccata
     7741 tgatgatgaa gtaataaaga aattaagaga agtaaatttt aaggatgaat taaacaaaat
     7801 tccttgcttt tattgtcgtg gggcatggga tgagacaaaa atgtctttta tagattatac
     7861 tctttgtagt ttttttaaaa aatctatagc taaaaaagaa cgtcaaggtc ttaaaccagc
     7921 agaagcacct ttattgtctg gagaagcctt tgattggtgt aataaagaaa atttaactcc
     7981 aatacttgat tatataagag aactttaaga ggtatatatt tgtaattatt tattatctaa
     8041 agttagattt catagaaact tactagacat tatttataaa ataatattat
//
LOCUS       DAMJZE010000172         8051 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJZE010000172 DAMJZE010000000
VERSION     DAMJZE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.176"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(109..960)
                     /locus_tag="PC541_003175"
     CDS             complement(109..960)
                     /locus_tag="PC541_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2840615.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1009..2178)
                     /locus_tag="PC541_003176"
     CDS             complement(1009..2178)
                     /locus_tag="PC541_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2840616.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2649..3566
                     /locus_tag="PC541_003177"
     CDS             2649..3566
                     /locus_tag="PC541_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2840617.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3695..4624)
                     /gene="pfkB"
                     /locus_tag="PC541_003178"
     CDS             complement(3695..4624)
                     /gene="pfkB"
                     /locus_tag="PC541_003178"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2840618.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4893..5618
                     /locus_tag="PC541_003179"
     CDS             4893..5618
                     /locus_tag="PC541_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2840619.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5701..6855
                     /gene="nagA"
                     /locus_tag="PC541_003180"
     CDS             5701..6855
                     /gene="nagA"
                     /locus_tag="PC541_003180"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2840620.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(7564..8034)
                     /locus_tag="PC541_003181"
     CDS             complement(7564..8034)
                     /locus_tag="PC541_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2840621.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
BASE COUNT         2823 a         1165 c         1023 g         3040 t
ORIGIN      
        1 tttcatcaat taaatgaaaa aaatacacca tttacatata attaatataa atttttcata
       61 taataaatat cctaaactta tctaaaataa atttaggata ttttattttt atactctatt
      121 tacagaccca cacactttaa tcttttcttt cacaaaatcc tttactgatt gaagccctgc
      181 taatccatac tttttagggt ctataacctc agaatcttca tctaaaactt ttttaacccc
      241 tttactatat gcaattctta attcagttgc atagttaact tttgcaatac ctctttttat
      301 agcttctttt ataatatcat catgtattcc tgaacctcca tgcaatacta aaggtattga
      361 aactacattt ttaatttctg ataacctatc taaatctatt ttaggttcac ccttatacaa
      421 tccatgtgca gtacctatag caatagccaa tgaagatatt cctgttctat cgacaaactc
      481 ttttgcctct tttggatttg tataatcatt actattcccg ccatctaaat catcttcttt
      541 tccaccaact ttcccaagtt ctgcttccac tggtataaga gaaggactac atgcttttac
      601 tactgattta gatatagata tattattttc aaagctttca tgagaaccat ctatcattat
      661 tgatgtatat ccacttctta aagctttcat tgcaaggtca aaacttgaac catggtccag
      721 atgcattgct acaggtatat ttactctttc tgcagcaacc tttgcatttg ctagataata
      781 atcaagacct gcatatttta cagtagatgg agtagtttgt aatattactg gagaatttaa
      841 ctcctcagca gcttctataa cagcaattac catttccata ttttctacat taaatgctcc
      901 tactgcatac ccttcatctt gtgccttttt aagcattgat gttgttgtta ctaatgccac
      961 attgataccc tcccaaatta tttagttata atattttaat cttatctttt aatattcata
     1021 tattgttaca ccttttacta ctcgatttac tgttcctgta gctgatggag tatctggctt
     1081 atttccaacc tttactgata caaacaatgc tatagtttgt gcaaacaata tgtatggcat
     1141 tgcaagatac acatctggta atttattata cttttcttta aactcaaact ttgttcctga
     1201 gaagttattt tcattttcta ctgacactgc acaagtgaat ttagcaattt tatccctttt
     1261 tatctcctct aaaacatcta aatcatattt tcttgtatat aaacagttag atacaaattc
     1321 aaatacaagt gtattttcat caataaatga ttttggtccg tgtctaaacc ccataggaga
     1381 atcatacact gtagatattt ttcctgctgt aagttctaaa agttttagtt gtgcttcttg
     1441 tgtcaaacca cctaatcctc ctgaacctaa atatacaact ctatcaaaat ctttatttat
     1501 taactcatgt atttcatctt ttctatctat aacatttctt cccatttcaa ttattgcatt
     1561 tatatatgac ttttctacat catcttctaa actatcgaat attaacatag cagataacat
     1621 catacacgaa aaacttcctg tcatggcaaa gccttcatca tttgattttg atggcattaa
     1681 taacaagtaa ttattttcgt cgtttttagt catcttagcc agttctcctt ctggattaca
     1741 agttatagct aaatgataaa aattatctac aatcttattc cctaaattta aagctgctaa
     1801 actttcagga ctatttccac ttcttgcaaa tgaaattaat aatgttggta tatctttttt
     1861 taagtaatca tatgggtttg aaactatatc cgtagtaggt attgcttcaa atatgtattt
     1921 tcttatgtca tttttatttt taaggtaagg aagtattgaa tttcctatgt aagctgaagt
     1981 tccagcacct gtaaatataa ctctaaactg cccatgatta ttacttattg tatctatgaa
     2041 tctacttaat ttttctttgt tagatttata tatttcataa gtttctaacc ataattcagg
     2101 ctgttgtttt atttcattag ttgttattat tgcacctaaa tcttttagtt tttgttcttc
     2161 taatttaaac attttcattc tcctttcata cttgtaacgt ttacatttgg taatgaggtt
     2221 ataaccactt taaactataa aaaaatatac tcttttataa ttttttgctt agatttttta
     2281 gtactactta aactcactat aaaatataca ctatagattt taattaattt tatagtttat
     2341 ctttaaaaat ttaataaaat catactctat atactttagt atttttattt tacgataaaa
     2401 gcttgttcag accaaagtgg taattaccac taatccaaat ataaattaat ttgaattttt
     2461 tgtcaataca taaaatattt atatcaatca attttatgga tatcctacaa aattacttta
     2521 tcaatatttc ttatttcgct aatttactta tataagcttt aaatttattt tttgatacaa
     2581 agtttacaaa tttatgtgtc tgaattataa tcaattctgt aaatataaaa accttaggag
     2641 aaagtattat gaaaaatata aaaatgggaa tgataggtct tggctccata gctcaaaagg
     2701 catatttacc tatattaact aagtctgaga gatttgagtt tgtaggagct tttactccaa
     2761 ataaagttaa aagggaaaaa gtctgtagtg attatagaat catgccattc gattctattg
     2821 aatccctagc taaaaaatgt gattgtattt ttctacatag ctctacagaa acacactacg
     2881 aaattataaa aatattatta aatttaggtg ttcatgtata tgtagacaaa cctcttgctt
     2941 caacagtaag tcaaggagaa gaacttatcg agttaagtac gaaaaaaaac ttaaatttaa
     3001 tggttggatt caaccgtaga ttctgtccta tgtacaaaga aattaaaaat aatgctacag
     3061 aaattgtatc tatcaatata tgtaaacatg gattaaattc cttaagaaat gttagattcg
     3121 acagtactct tatagatgac tatatacatg ttattgacac tgctttatgg ttagcaaatg
     3181 aagatgttga aattagtgga gaggatttat ttttaacaga caataagaat cttatatttg
     3241 taagtcataa acttaaaggt aaaaatttct ctataaacac ctctatgcat agagattctg
     3301 gaacaaagtt agaacaagtt gaaattcttt caaagggtaa gattcaaaga gttaagaatc
     3361 ttaatgtact ggaaatagaa gaaggtggaa acctaacttt aaagcaaagt ggtgcatggg
     3421 taaatatatt aaaacaaaag ggttttgagg atatatcaaa tcattttatt gattgtatag
     3481 aaaataatat aaaaccagct attaatggag aagagtgtat caaagctcaa agattactag
     3541 agaaaattat aaattcagta aaatagatat ttattataaa aataggttaa aaatgaattt
     3601 ttctaaccta tttttatata agactatatc ttttatatta aaattaaaat ctattacaac
     3661 aacattaaat ttattatatt cccattaaat gtatttaact taactttttt acttcaacta
     3721 aatcttgata tttatcaatt tctcccaaat ctacaaatcc agtttccata tttaaagcat
     3781 tacttatacc acaagcattt gcaaatttaa gtaaattttc tattttataa ccccttaaaa
     3841 tacctacact aaatccagcc actgtgctat ctccactacc tacagtattt atacacttta
     3901 caactggtac ttttacatca tatacattat cttcgtttaa atacaccata ccatcttttc
     3961 ctaaagatat acatacattc tctgcaccca tttcaataag tttttttcct gacaagataa
     4021 tttcctctct tgattctata ttcattccta ataaatgtct tatttcatct atatttggtt
     4081 ttattaaata tggttttgat tttaaagcta tttttaatgc ttctcctgat gtatctaata
     4141 taaactttat attatttgct ctacatatat ttcctatctt ttcataatag tctatcggca
     4201 tattctcaca ataactacca gaagctacca gtattttagt attttttagt acttcctcta
     4261 tatcattttc aaatctctct aaatcgcttt tttctactat tggtcctttt tctaaaaatt
     4321 ctgtactaac tttattgtcc tcaattatgt ttaagcaatt tctagtttcc tgctctattt
     4381 ttgtaaattt attttgtata tctagttttc taagttcatt ttttataaat tctccattaa
     4441 atccgcctag aaatcccata gctaaaggat tttcttttaa aattttacat accttagcta
     4501 cgtttaatcc tttacctcca gcagttgcat tcttacttac taccctttgc acctcaccta
     4561 tgtttatatt attcactcta tacattctat ctatagatgg gttgaatgtt attactgtaa
     4621 tcatactaga ttctcctcat tattaatttt tacttttcta aacaataaat taatctgtaa
     4681 aataaaaaat acctcatcta taatataccc aaagtggtta ttaccaattt gaatataaat
     4741 tattttactt gttttgtcaa tgtaccataa ttttataata aattaataac cttacttatt
     4801 gaattttgat taaactttat tcattgacat aaataattct atattctata atcaaattgt
     4861 ggtaataacc acttagaaag gactatattt ttatgataaa aagaaatgat aaaagaccaa
     4921 tatatgacca gcttgttgag atattaagaa ctaagattga aaatgagatg gaaccaaacg
     4981 atagaatgct ttccgaaaga aaaatttgtg atgaatatgg agtaagtaga accacagtta
     5041 gactagctat ggctgagttg gaacatatgg ggtatatata caaaagacat ggaaaaggaa
     5101 cttttgttgc tgcactaagt caaaattctc aaaacttaat ggaaagttat agttttacag
     5161 attatatgag aacacaagga aaaaagccag gtacaaaggt attatcattt gaggtagtag
     5221 aaagtacaaa gtatttctct gaaaaattag gtataactcc tggtgaaaaa atgataaaaa
     5281 taatgcgttt aagacttgct gatgaccttc ctatgatgct tgaaagaaca tatcttccta
     5341 tgaaagaatt tataggattg acaaaagaaa aaatcgaaca aaaaccttta tatgaaatat
     5401 ttagagaaga ttatggcgag attattaaag ttgctgatga agaattttca gctggaataa
     5461 tctctgataa ggaagctaag ttattggaag taccaggaga ttcaccttgt ctgaaattac
     5521 ttcgtaccac atataatgat gacaatatag ttattgaatt tactttgagt gttgctagaa
     5581 gcgataaatt tgtatataga gtaagacatg taagatagta agttaaataa ctttcaagaa
     5641 aatagaatga tattataaac gtcaatgcca caatgatagt ataattataa ggaggttatt
     5701 ttgaaaagtt ttatttatgc agataaattt tttatgaaac atggaatcaa agaaaaaggt
     5761 taccttagta taattaatgg caaattcggt gaatttcaac aagaaagacc taatgttact
     5821 gataatgtta tagatttttc aggtaaatat attgctcctg gcttagttga tactcacatt
     5881 cacggtcttt taggtgcaga tgtcatggac aatacatttg aagcaataaa cacaatttca
     5941 aaaggcttat taaaatatgg agtaacatct ttcctaccta caactttaac tgattctgta
     6001 gatacactaa gtgattctgt agaaaatata agcaatagct tcaatcgagt tgagggtgca
     6061 aaaatacaag gtatttattt agaaggtcca ttttttacag aaaaatataa gggcgctcaa
     6121 aatgaaaaat attttaaaga ccctgacatc aatatattaa aattatggca aaaaaaatcg
     6181 aaaaatttaa taagaaaaat agcaattgct ccagaacgta aaggttcaat agaattaaca
     6241 agatatgcaa ctaaaaataa tatatcagtt tctcttggtc atagttcagc tacatttgag
     6301 caagttaaac aagtcgttaa ttcaggtgct aaagtgtttg tacatacata taatgggatg
     6361 agtcctttaa atcatagaga acctggaatg gttggggctg ctatggtact aaaagatact
     6421 tatgctgaat taatttgtga tggtcatcat gtaagcccaa tagctgttaa aataatgatg
     6481 gatgcgaagt ctagtaataa catagcttta attacagatt gtatgagagc tggtgctatg
     6541 gaggatggaa aatatactct tggtaagttt aatgtaaatg taaaaaatag tgttgcaaga
     6601 ttaaatagtg ggagtttagc tggaagtgta ttaactatgg acaaggctgt gagaaatata
     6661 gttgattgga acatatcaac tttagaagat gcaataaaaa tggctacata tgtccctgct
     6721 ttaagttgta atattgataa tgtctgtggc tctatatatc aaggtatgga tgcagacttt
     6781 atagtaacag ataatgactt taatatacac gaaacttata taaatggtat ctgtaagtac
     6841 aaagttaata aataggttca atataaggtt atggaggctg tctaaaaaga gtctggaaat
     6901 atttaaagtt atttctccaa aatctgattt aacactttta tttccatatc gttaataaac
     6961 tattgcgata gaggttattt taaacaaata tatattataa gtatttttgt gataatattt
     7021 tcgataatat gtacatttaa agtaactttt ctattattat ttgtaagttt ttatcttgag
     7081 caaacatata aatttttaat aaagcatgag ataaacccta tatttcaatt actaatgatg
     7141 agattttgat tgataacaga tatattttca aatgtaaaaa tactaaaaat catatcaatt
     7201 ttaaagatat tgtaggtgta aataaacaag ataatactat agaaattttt acaaatgaaa
     7261 gcacttattg tatatatcta aaagttttaa gcattgagag cgaagaatat ctaatagata
     7321 gactaaaatc attttgttga ttttatatat tttatgttaa atataaagca gagtagttaa
     7381 aactaccctg cttttgtaat taaaatttaa aaataataac tcataataca ttgaatttga
     7441 gcttaacagt aactattgta ataatagtat caatcttttg tgttgaatat aatttattta
     7501 cactatagtt aagcatgatt tctaatgtta ttttctctaa tttacaatta tgattaaaat
     7561 tcattaatat atatatttat aagttgtatt gtttatattg tttattactt ttttaaatcc
     7621 actagctgta ataataaatg tcttttctgg tatccctggt gaaaagatta ttttatcatg
     7681 gttaaatata tcttgatcta ttacaagaga gatatttttc tcatatccaa aaggagatac
     7741 acatccaggc aaacattgtg ttttttgtgt taattcttct tttgaacata tagatacttt
     7801 tccacctaaa acatttttta tagtttttgg atctaatttt tttccttctg tggttacaaa
     7861 tatatagtaa tttccagatc tatcttttat aaataaactt ttactttcta ctccagtata
     7921 ttgtaatttt tctcttattt ctttagcttt ttcaaatgat aaaataggtt catggtcata
     7981 ttcttcaaac gttatatgat tttcttttaa aatatttttg atattgttgt acatatattt
     8041 tcctttctat a
//
LOCUS       DAMJZE010000173         7928 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJZE010000173 DAMJZE010000000
VERSION     DAMJZE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.177"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(31..744)
                     /locus_tag="PC541_003182"
     CDS             complement(31..744)
                     /locus_tag="PC541_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2840622.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1036..2700
                     /locus_tag="PC541_003183"
     CDS             1036..2700
                     /locus_tag="PC541_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2840623.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2807..3706)
                     /locus_tag="PC541_003184"
     CDS             complement(2807..3706)
                     /locus_tag="PC541_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2840624.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3733..4371)
                     /locus_tag="PC541_003185"
     CDS             complement(3733..4371)
                     /locus_tag="PC541_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2840625.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4708..5706)
                     /locus_tag="PC541_003186"
     CDS             complement(4708..5706)
                     /locus_tag="PC541_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2840626.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5804..6379)
                     /locus_tag="PC541_003187"
     CDS             complement(5804..6379)
                     /locus_tag="PC541_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840627.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            6695..6958
                     /locus_tag="PC541_003188"
     CDS             6695..6958
                     /locus_tag="PC541_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840628.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(7395..7772)
                     /locus_tag="PC541_003189"
     CDS             complement(7395..7772)
                     /locus_tag="PC541_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2840629.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         2789 a         1219 c          837 g         3083 t
ORIGIN      
        1 taaaaatata aatataagtt ttctatattt ttaaccaaca gatgttttct tcaaattaat
       61 cagatgattt agtatatctt gaacctttcc agagtccttt aaatatccta atgccccatt
      121 tatttgtgat tttaaatcta tttctacttc catatccaaa tcaacatttc ctttttttat
      181 agatatattt ttaagtgtta ttgggtaaag ataacttttt tccaaagtaa tcttatttcc
      241 attaacctca aatggaactt ttttattgta gttacttaat attttttcta gcattttatc
      301 acttagttta aattttccaa ttttcacatt ctcaagactt acaactaagt ttccatcctt
      361 taaagtaggt ctcaattcaa gttcatattg actatttacc aaaccaaata ccttatatgg
      421 tcctgctacc tttattttac catttttcat ttcaacaaaa ttattttcaa gttcattaat
      481 tccatgcttc tgcatcaatg tatatattaa atttttaaac tcttcctctg aaacagatat
      541 ttttccaacc attctaagtg gattttttac tacctccata gaatttatat atgagccttt
      601 tgataaatat tcttcatcta tgattttatt attttgtgaa ctaacatcat atctttcttt
      661 tggtgtcaag atatatacaa caattcctgc aagtactaaa acaataacaa ttaaacttaa
      721 caaaatcttt gcaacctttc ccataatcgt ctcctttaca aatctgtcat cagatttaaa
      781 ataaatttag ataactaact tcacataata ttcttaacaa tatacatact ataatacagt
      841 cttttaaaaa ttaattcaac caatctaatg ttttgtaaat aaactgtaaa aacatgtttt
      901 tagtataaaa gttaatattt tgcataatac tcttaattac acctttattt ttttctaatc
      961 ttggattaaa attaatatta tgcaatacag tgtagtgttc tgtattataa tcaaattaaa
     1021 taggaggtat agaatatgtc aaatgaaacg aactcatcta actttataaa gaatattatt
     1081 ataaacgact tagaaacagg aaaacatgac agtatcataa ctcgttttcc acctgagcca
     1141 aacggatatt tacatattgg tcatgcaaaa agtatatgtc ttaattttgg attagctaag
     1201 gagtttaatg gaaaagctaa tttaagattt gatgatacaa acccattaaa agaggatgtt
     1261 gaatatgtag agtccataaa agaagatgta aagtggttag gatttgattg gaacgagtta
     1321 aattttgcat ctaactactt tgatgaaatg tataagagag ctttaatact aataaaaaag
     1381 ggtaaagctt atgtatgtga tttgacacaa gaggaaatga gagaatatcg tggtactcta
     1441 actgaaccag gaaaagaaag tcctcataga aacagaacta ttgaagaaaa ccttgattta
     1501 tttgaaagaa tgaaaaatgg tgaatttaaa gatggtgaaa aaacattaag agccaaaata
     1561 gatatgtctt ctccaaatat aaaccttaga gacccaatta tatatagaat atctcattca
     1621 acacaccata atacaggaga taaatggtgc atatatccta tgtatgcttt tgctcatcca
     1681 attgaagatg caattgaagg aataactcac tctatatgta ctttagagtt tgaagaccaa
     1741 agacctcttt atgattggtt tgttaaagaa tgtgagatgg aaaatatacc tagacaaata
     1801 gaatttgcta gacttaatat aaataatact gtcatgagta aaaggaaatt aaaacaactt
     1861 gtagatgagg gtattgttga tggttgggat gaccctcgtg ttcctacaat atccggaatc
     1921 agacgtaagg gatatactgc agaagcattg cgcaatttct gtagtgaaat aggggtatca
     1981 aaagttaact ctacggtaga tagtcaaatg cttgattatt tcctaagaga aaacttacaa
     2041 ccaaaggctc ctcttactat gggagtttta agacctttaa aacttattat aactaattac
     2101 ccagaagata agattgagat gcttgaaata gagaataatg ctaaagatga atctcaagga
     2161 aagagattag ttcctttctc tagagaattg tatatagaac aagatgattt tatggaagaa
     2221 cctgttaaga aatattttag atttttccca ggaaatgaag tccgtttaaa aggtgcttat
     2281 tttgtaaaat gtactgatgt aattaaggat gaaaatggaa atgtagttga aatacatggt
     2341 acttatgacc ctgaaactaa gagtggctct ggctttacag gacgtaaggt aaaatctaca
     2401 atacattggg ttgatgctaa atcagctata ccatgtgaat ttagattatt tgaaccatta
     2461 attcttgatg atatacctga aaatgaaggt aaacatttct taaaacaaat aaatcctaat
     2521 tcattggaaa tattacaagg ttttgttgaa cctacacaga taaaagatgc taaacctttt
     2581 gataaattcc aatttgttag aaatggattc tttagtatag ataataaata tacaactgat
     2641 gaaaaatttg tatttaatag aatagttcct ctaaaaagtt cttttaaacc aggtaaataa
     2701 tcttacaatc aaaaaatgaa gacttaaaat ataaattact tctactataa ataaacttag
     2761 ttagaagtaa ttttttattt gagtcttcat catttacttt aaatgattaa cttttttcac
     2821 tacagattcc catgacttct attccatctt ttatgcattc tatgttaaga ggaaaatctg
     2881 gtcctttttc tccgtcaata tctgttataa gtgaatcttc acattctata tgcacctttt
     2941 tagttttaaa gtatagtatt tcatttccat tatattgatc taaataatca cctctaagca
     3001 cacttatcaa aactggtatt gattttggta ttggcattcc tttaaaaata attacatcca
     3061 aatatccatc atctatttca gccttatatg ctagatttat atttcctgct gttttgccat
     3121 taaaaatcaa cataagatac atatctccat cataatacat ttcctcagaa gatactttta
     3181 tcttaaattt cctcatatac aatgcttctt ctattccctt tatataataa gaaattcttc
     3241 caaatgaatt ttttaagtct gtgtttatcc tttgtgatac atccgtaaac ataccagcac
     3301 ttgcaacatt tataaagtac ttattattga ctttccctat gtctattttc tttggttttg
     3361 aatttatgat ttcatttata gcttctttta tatcaaatga aatttgaaga gcatttgaaa
     3421 aatcatttgc agttcctgta ggcaaaattc caattggaat ttttatgtct aattctttca
     3481 ttgcatttac tactaaatct acagtaccat caccaccagc tatcaatatg tgttcataag
     3541 aactatcaac ttccttaaat gcattcttta caggttcttt aatagttaat ctatagggta
     3601 tcaataaata atttttttct tgatataatt ctatgatagt gtctaaattt gataatatct
     3661 ttttttcacc tgaattagga ttgtatataa gttttacttt tttcattatt tctccttact
     3721 cattttattc ttttagtatc caaaatcttc atttaaaaaa atcacatgta ttctttctgg
     3781 tgttaattgt ctttttataa gtgtataact acagcatatt cttcctggac ttgaacagtc
     3841 tatacatata ccagtagtct tacatccagt atttgtactt aatcttttac aattggctgg
     3901 agcagctatt gtttttgttc tttttatagc ttcatctaca tctttaacta ttttatttac
     3961 tcctgctata actattactt tatcaggtcc atatatcata gcagcaactc tattcccatt
     4021 cccatctaaa ttgtaaagtt ctccatcctc tgttatcgca ttagtacttg taaaataagc
     4081 atctgcagaa aatgactttc tatatatttc tttcatttct tctggtttga tatcttttgc
     4141 atatctatca agaaaattat atttttctct tataaaatca atcactcctg tttcaaataa
     4201 ggtcatagaa cctccacatc ctactagaga acctgcttta actatttctt caattttttt
     4261 aagaagctct tctttgttac ttactaaata ccctttcata ttattgtttt ccaaagattt
     4321 tattgttttt tctattcttt tttcattaag ccaatttaaa ttttgatcca ttttaacttc
     4381 ccccttattt ttaatatgca cttaattatt tataaacttt attttcttta taagtttatt
     4441 ttagtacatc taaaaaaaaa tacaatataa aacttaaata gttttacata attttttcca
     4501 aaataactta ctcaatcaaa aaaggaaagt agatgtacta ttctactttc cctatattgt
     4561 atgtagtatt tattgcctaa aatctatata ttaattatat gtttcatatt atataatatc
     4621 acagttaatt aaaatgtaat actatcttct ttttaaacat ataaattgta ttattaaatt
     4681 taatatatcc aacttcaata aatcttatta atttgatgaa gaagatgcta tagctgcact
     4741 tgttgctgcc attacacatg caatttgaat agcaacaaca atatttaaaa atatattatt
     4801 agttatagag cctatattat tctcatttcg tatcaaatct gcataaattg aagctacaat
     4861 agcagaacta atcattactc tattactttt tcctaaagac caatttccaa agcctttttc
     4921 ttctttaagt ttatttgaaa cattttttat ctcttctatt atttcatctt ctctataatc
     4981 taataatgaa attgctccta ttattggata tccataataa ttcattttac aatcattttc
     5041 tttaaatgct tttttaagtt caatagtctt tttacattta agcatagttc tatcatcact
     5101 aaataaaaga atttgggata atgcttgtaa atcatttcct ttataaaaac cattttcatt
     5161 tagaaattca taacattttt ctatgtattc taaatcttca tctaaatttt ttgaatgaat
     5221 tgcaataagt gctatattac aatagtcatc actagaagtt agaaatggat gttttttcct
     5281 catgtaatca tatattattt tcatttttga tatacatgta agataatcca ttttctctct
     5341 attttcataa actattgatg ctgtaagtgg taaatatgta tcgccccaaa acttttcact
     5401 tttaagttct ttgtatatat ctaaaatcat cttaaaacta ctttctggat ttgattcaaa
     5461 cgaaagtagt gttgccatat aaaacatatt atatcctcta aaattagaga ataatccagt
     5521 atttttttta ataagctcta ttcgttcttc cactaatcta ggacttattc tcttgttctt
     5581 aagggtaaat gcaagtgcac aactatgttg tatcaatccc atttgccaag aacctttaac
     5641 ttcatttaat gctttgtaat tttctattaa taagtttact tttccattta taaacgattg
     5701 ttccatattg ccctcctagt aatatatctc attgtactaa tagttttata tttattatat
     5761 atatattaaa tactacaatc aatacatatt acttcaaaga acatcattct tcaacaataa
     5821 tgtagccttt tttgtaatgt cttcccatat tattgactct atcatagtac tctgattcac
     5881 tttcattact ccatttgggc acaaatacat ctattgcatt agaattacta aaatacttac
     5941 tagaccccct atccataaca gtttttaatt ttccatctac aataatctta gttccaaaac
     6001 ttaagtgatt gtttgctaca aaaccatctt ctaaatcatc tcctgtagca gttatcgctc
     6061 catatccatt ttcttgatta agacttgtgt aataactaac ttcaaaagtt ataggtacta
     6121 acttctttat tttaatacct aactgatttt gtagtttctc cttttgtact ttcaacttat
     6181 actcatcact atttttataa tcttccatac cttgattata cttggaatta aagcttttac
     6241 ctgactttac tatagtatct atatttacta tagattcttt acaatgatct gcatctattt
     6301 ttgtttctcc atttaccaat aatggagtta aacatacact acatacaaca catgtaatca
     6361 atgcttcttt aagcttcata aattcccccc ttattatttt gatatattag catatttaat
     6421 attatatgtc tacatttaga tttaaatacc acacccattt gctttaagaa gttgtcttat
     6481 atttaattca ccttaaacac ttataaatcc tctatcttag aatcatatag aataaagctt
     6541 ttatctttaa atatttttta ttaatctttt aaaatctaaa actactttat ctaattttct
     6601 cttttttaga atttaagaat taaaattcta taattttaaa acatattgaa tttatttttt
     6661 tgagatgata taattttctt aagaggtggt tgatatgata aaaaatacaa aacaaattac
     6721 agtagaagtt aatgaaattt tattacaaga aatgtctaag ataactaatg acgaaaataa
     6781 tattttgaca gatttgataa atgaatcaat tgtaaattat attcttgaaa acaaaaaaca
     6841 taatattgaa aataaagatt tagaagaagt gattaaattt tacaaaaaag aaaatactga
     6901 aaaacctaaa agtgcagcaa gagctatagc tgaagcaaaa actcatactc tgtattaatc
     6961 aaataaaaaa gttcctaaaa actaggaact tttttatatt aataattatt aatatatctc
     7021 aatatttatc tcaaatctat caatataagc atgtttaata caatctattg tatacttatc
     7081 ttaccctaga aataatcaaa acaaaaataa aattatgccc tttatatcta aaacaattaa
     7141 tttacatgat taatatacaa aaattctgac ttttaaattc gtatacaata tcaaactaaa
     7201 ataatattaa tgacaaaccc aatataaatt aaattcaaac aggtttgatt ttacatcaaa
     7261 cttattgtat taatattaaa taacaagaca aaaatattta taaaaatcac aaattttaca
     7321 taaaacttta tatttgattc atttaactaa ttattgtaaa aactatttat tttatgataa
     7381 ttaactacac caaattattc atctttttta atatccttaa cccatttttc aacagagttc
     7441 aattcttctt catttacccc ctttttgaac aaggatttca taaaactctc aatagaactt
     7501 ccatgcatat tatctaaaaa agtttttgtt tcaaaatgta aatactcttc ttctccaata
     7561 agaactgtat aatgtgtatg tctatcaatt tttcttgcac ttaagaattg cttatttact
     7621 aatcttgata aaagtgttag tgttgtagtt tgcttccaat tatatagttt ttccatagct
     7681 tcaatgacat cttttgatgc tactgtaaaa tctgaactcc aaataaattt catgactttt
     7741 aattctgctg gtggtatctt agtaatagtc atatgatttt acactcctct caaacttcta
     7801 cataattaaa tactatatta atagactttt cgagtcaaag gttttattta aaatatctca
     7861 tccaaattta ttaatacaaa aaatttatat ttttattcaa aattaaacac acaatctatt
     7921 aatataaa
//
LOCUS       DAMJZE010000174         7914 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJZE010000174 DAMJZE010000000
VERSION     DAMJZE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.178"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            27..101
                     /locus_tag="PC541_003190"
     tRNA            27..101
                     /locus_tag="PC541_003190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC541_003191"
     tRNA            116..200
                     /locus_tag="PC541_003191"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC541_003192"
     tRNA            210..293
                     /locus_tag="PC541_003192"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC541_003193"
     tRNA            317..391
                     /locus_tag="PC541_003193"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC541_003194"
     tRNA            416..492
                     /locus_tag="PC541_003194"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC541_003195"
     tRNA            502..577
                     /locus_tag="PC541_003195"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC541_003196"
     tRNA            586..661
                     /locus_tag="PC541_003196"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC541_003197"
     tRNA            664..752
                     /locus_tag="PC541_003197"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC541_003198"
     tRNA            756..831
                     /locus_tag="PC541_003198"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC541_003199"
     tRNA            838..911
                     /locus_tag="PC541_003199"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC541_003200"
     tRNA            926..1002
                     /locus_tag="PC541_003200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC541_003201"
     tRNA            1020..1096
                     /locus_tag="PC541_003201"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC541_003202"
     tRNA            1105..1180
                     /locus_tag="PC541_003202"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC541_003203"
     tRNA            1188..1261
                     /locus_tag="PC541_003203"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC541_003204"
     tRNA            1269..1345
                     /locus_tag="PC541_003204"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC541_003205"
     tRNA            1358..1433
                     /locus_tag="PC541_003205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC541_003206"
     CDS             1509..1922
                     /locus_tag="PC541_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840630.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC541_003207"
     CDS             2231..5662
                     /locus_tag="PC541_003207"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2840631.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC541_003208"
     CDS             5904..7832
                     /locus_tag="PC541_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2840632.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3098 a         1011 c         1592 g         2213 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttgaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttca ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaagagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aaaatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttag
//
LOCUS       DAMJZE010000175         7902 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJZE010000175 DAMJZE010000000
VERSION     DAMJZE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.179"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(195..950)
                     /locus_tag="PC541_003209"
     CDS             complement(195..950)
                     /locus_tag="PC541_003209"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2840633.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1390..2379
                     /locus_tag="PC541_003210"
     CDS             1390..2379
                     /locus_tag="PC541_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2840634.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2735..3451)
                     /gene="dapD"
                     /locus_tag="PC541_003211"
     CDS             complement(2735..3451)
                     /gene="dapD"
                     /locus_tag="PC541_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2840635.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3675..4424)
                     /locus_tag="PC541_003212"
     CDS             complement(3675..4424)
                     /locus_tag="PC541_003212"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2840636.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4491..5378)
                     /locus_tag="PC541_003213"
     CDS             complement(4491..5378)
                     /locus_tag="PC541_003213"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2840637.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5495..6496)
                     /locus_tag="PC541_003214"
     CDS             complement(5495..6496)
                     /locus_tag="PC541_003214"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2840638.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6921..7802)
                     /locus_tag="PC541_003215"
     CDS             complement(6921..7802)
                     /locus_tag="PC541_003215"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2840639.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2699 a         1287 c         1026 g         2890 t
ORIGIN      
        1 gatgaagaga ttataaaata ataaacaata taattccata taccaataaa taagataatc
       61 tttaagaaaa atgattatat ttaaaaggta aacataaaat ttataaactt ttagaataga
      121 aatctcaatc aattagatat tcaaattgat gaatatgaat acctaaatag attgagattt
      181 attatgtaca aaacttatcc taatatatct gacattttat aaagcccaga ttgcttacca
      241 attaaaaatt tagctgcaac tatagaaccg tttgcaaata cttcatcaga ttgagaatga
      301 tggcttaatg taagaacttc attatcacct gcaaatacaa catcatggtc gctgattata
      361 ttgccacctc ttatagagtg tattccaatt tcatttggtt ttcttttaca atctctacca
      421 tatctaccat gtgtattttc actttcaggc aagacttctc taattgcatt agctatcata
      481 gtagcagttc cactaggggc gtcttctttt ctattgtgat gtttttctat gatttcaata
      541 tcaaaacctt ctaacatctt tgaagcatcc ttaactaatt ttattaatat attcactcca
      601 agagatgtat ttgatgataa taatatagga atgttttttg atgcttcttc tattttattt
      661 aagtcatttt catcatatcc agtagtagct aatactacag gtgtattatt ttttagagca
      721 tatgacaata ttggctctaa agtctcatga tgtgaaaagt ctattattac atcagttttt
      781 tcagttacat cagtcattgt tttgtaaagc tttgtatcac tatgatgacc tttttcatca
      841 gcagacctag ctacaccaca aaccaactct aattcattat ctttttttac acattctgct
      901 aaaactcttc ccattgttcc attatagcca ctaattataa ctttcaacat aaattcctcc
      961 tatgaaatta ataaaaattt gttatatttc tataagttta ttttatcact aatataattt
     1021 atataaaata atattagtga taataagctt acaatttatc ttacattaaa aaatttaagt
     1081 ggatttttca aaatatagat aaatttcttt ttcattgtat aaatgataaa aatatatttg
     1141 acttcatacc aaaaagagtg ccccatgaac taaaaagtta attttgaaat accatattca
     1201 tatatcatat gatttatttg gaaatcaata ttaatacatt aaaataacta aatacatgta
     1261 tgcataaaga tgtataaaaa atatataaac atatagtatt aattttaaat tcaacaattt
     1321 aattgttaaa agtgttatta ttaaattaga aatatcttaa cttaatatac tgagatggga
     1381 gtgatagata tgttaaaaga agaaaaaact tatcaagaga aagacgcaaa agtattcaat
     1441 ggagtggcag ctttgataat gattttatta tttttagtag tttcaatagg tatcatggtt
     1501 tttgggatag taaaacttga tagtggattt actggaatag gttcacttat gttaattttg
     1561 ggacttgtat ttatagtagt agattttatc ctattttttg ggctaaaaat gattaatcct
     1621 aaagaagcta tagttctagt tttgtttggt aactattatg gaactataaa aaaagaaggg
     1681 tactattggg tgaatccatt ttgttcagca ataaacccag cagtatcaag aattaatgta
     1741 tcgaaatctt catctgatga aaaggtagat atatcaacta attcaagggg caaaaaagta
     1801 tctttaaaaa caatgactct taataatgaa aagcagaaag tcaatgattt attgggaaat
     1861 ccaattataa taggagtggt tgtaatttgg aaggttatag atgcaacaaa ggcagtattt
     1921 aatgtagata attataatac attcttatct attcagtgtg attctactat tcgcaatgtt
     1981 tcaagacttt atccatatga tgtatctgaa gatggagatg aaaaatctct tcgtggtagt
     2041 agtcaggaaa tagcagatag attaaaagat gaattacaat caagggttga tattgcagga
     2101 attgaagtgt gtgaagtgag aattactcat ctttcttatg caccagagat tgcagcagca
     2161 atgttacaac gtcaacaagc agaggctata atagcagcac gtaaaaagat tgttgagggg
     2221 gctgtaagta tggtagagat ggcattgact aatttaagtg aaaataatgt tgtgactttg
     2281 gacgaagaac gtaaagcagc tatggtaagt aatctgttag ttgtgctttg tggcaataaa
     2341 gatgcacaac cagtggtaaa cagcagttct atttattaaa ttataaaaag tttaatagtt
     2401 tattttattg atatatagaa aatttagtag ttcattttat taaattatat aaagattaat
     2461 ggttttcact ttattaaatt ataaaaagtt taatagttta ttacattgat atattgaaaa
     2521 attgaaggaa ataaaaatac ttgtcagcaa gaaataaaaa tgatataaga atgaataaga
     2581 gaattgtaaa aaaataaaat gattataaat aaaagataaa aatattgtaa aaaagagggt
     2641 gcctctggtg aattaaattc acacagagac acctttttaa ttaatttttt aaattagtat
     2701 attattgttt aattaatagt atagtactat ttaattaatc taaatttcta aggtcttcca
     2761 taagcttagt tttgtcagcc gttttttcat ctttcatttt tataacttta gcaggagaac
     2821 cagcaacaac ggcaccagct tctacatcag tagttacaac agcaccagct gcaacaacag
     2881 cacctttacc gattctaaca ccttcaagaa taactgcatt agctcctatt aaaacatcat
     2941 cttcaactat aacaggagta gcagaaggtg gctctaaaac accagcaaca acagcaccag
     3001 cacctaagtg aacattttta ccaagagttc ctctagctcc aataacagca ttcatatcta
     3061 ccatagaacc ttcaccaata acagctccta tatttataac agctcccatc attacaacag
     3121 catttttttc tatagtaacc atgtctctta ttattgcacc tggctcaatt cttgcatgtt
     3181 cacttaaagt attttttaga ggaatagcag aatttcttct atcatattct acatgagtat
     3241 ctgttatatc atttccatgt ttatctaata cttccattat atcctcatag tcacctacaa
     3301 atatataaga accattactt ccgaatacct taaatttatc agttgattgg aagtgtaaag
     3361 agttagtatt tacatatacc ttaacaggtg ttttcttttt aacttctttt atataccttg
     3421 ctatttcata agcatcgttt aaatttacca tttgttaatt ctccttgtat ttcattatat
     3481 ttcttttatt atatacttat ttgtcaattt tagtatatac ttacttctta ttatttatat
     3541 tttattttag ttagttttta ttgtatagac atagtattaa ataaatttat tatatatgtt
     3601 tgatttgtat tgcgtattat tatatctata ttgcatatgt ttaatttata ttgtgtatta
     3661 ttatatttat gctattatcc caacatgctg tccatattat aaaatcctgg tttttgctta
     3721 actaagtatt tggcagcagc tatagaacca cttgcaaaga taccacgaga ttgagcttga
     3781 tggcttattg taagaacctc attgtctcca gcaaatagag catcatgttc gccaacaata
     3841 tttccacctc ttatagcatg aataccaact tcatttggtt gtctttttgc actacgacca
     3901 tatctaccat aattattttc aacgtttggc aaaacctctt ttatagcgtt agcaatcata
     3961 acagctgtgc cacttggggc atctactttt ctgttgtgat gtttttctat tatttctata
     4021 tcaaatcctt ctagtaattt agctgcatct ttaaccaatt ttactaatac atttacacca
     4081 agagacatat tatatgattg gaatacagga ataactttag ccgcttcacg tattttgttc
     4141 atttcgtcat cattatatcc agtagtagca attactaaag gtgttttagt ctttaaaacg
     4201 taagataaga tattatctaa attagagtga tgagaaaaat ctataactac atcagcttcc
     4261 tcaactatag tactcatatc ttcataaatt ttaaggtcgc cttcatagct atccagacct
     4321 ctagctgcaa cacaaaccaa ttctaattca ttatcttctc ttatagtatc ggctaaaatc
     4381 ttacccattt ttccatcata accagttgct ataactttta acataatttc ctcctatgat
     4441 taagggctaa cttttgacca aacttacttt ttaccatgca ttaatcttta ctatgcatta
     4501 acttttagcc caaagttaac taattctttc tttaatactt ctaaatttgc tgggtccatt
     4561 tctgctagag gtaatcttaa atctccaaca ttgaatccca ataaattcat tgcagtctta
     4621 actggtatag gatttacttc tataaataaa gctgcgatta gagcatccat accaagttgt
     4681 aattttctag aaccttcaat atcgccttca aagaatttag ctaccaaatc gtgagtttct
     4741 tgagggcaaa cattagcaag tactgatata actccttgac ctcctaaaga taataaagga
     4801 agtatagtat catcattacc agagtagata gcaaagtcat ctggaactaa actagctatt
     4861 tctgcaacct gtcctaggtc tccactagct tcttttacag caactatgtt atcaatttta
     4921 gcaagttcaa ctataaggct aggacttata tttacacaag ttcttccagg gacattgtaa
     4981 agtataattg gaagtttaac actgttagct attgtttcaa aatgacgctt aagtccagct
     5041 ttattagctt tattgtaata aggtgttatt attaatagag catctactcc taatttttca
     5101 gcttcttgac ttaagttaac tgagtgcata gtatcattag aaccacttcc agctataaca
     5161 ggaactcttt tattaacttt ttcaacaaca tacttaatag tagcaagttg ttcttcatca
     5221 gtcatagtat tagcttcacc agtagtacca caagatacta tagcatcagt accattagct
     5281 atttggtatt caactaattc acctaatttg tcaaaatcaa ctttgttatc ctttgtaaat
     5341 ggtgtaacta gagcaacagc agaaccttta aatatcatat aaaatcaatc ccctcttttt
     5401 tatttttgtg ttttatatta cagttacttc taatgcttgt ttttgagtta ctattaatat
     5461 tttacaaaat tatatattta tacaagtttg tatattatac taaatcgtat gataaagcca
     5521 tttcagctat ttgaattgcg ttagttgcag caccttttct tatattatca gcaacaaccc
     5581 aaagatttac accattatca acactaaaat ctcttcttat tcttccaaca aacacttcat
     5641 ctctatcatc aaattctaca gcagttggat attcgttttt agcaggatta tcaactacta
     5701 caacacctgg agcttcttca agtgttttta ctaaatcatc taactcaaat ggattgttaa
     5761 actcaacatt tatagattca ctatgaccat tttttactgg aactcttaca gtagttgcag
     5821 ttatttttaa atcgtagtta tcaagtattt tcatagtttc atttaccatt ttcatttctt
     5881 ctttagtgta tccattttca gtgaatgaat ctatatgagg aagacagttg taagagattt
     5941 gatgagggta gaatccagta ggattaccgt tcattccttt ctctaaatct tcaacacctt
     6001 taacaccaga accagataca gcttggtaag tagagtaaac aactctctta attttgtatt
     6061 tatcatcaag agctttaagg ggaaccatag cttgtatagt agagcagtta ggattagcta
     6121 ttataccctt atgatttttt atggcttctg gattaacctc aggaactact aaaggcacat
     6181 ctggattcat tctccattga ctagagttat ctactactaa gattccattt gaagcagcta
     6241 ttggagcata cttttcacta atacttccac cagcagagaa taatgctatt tgtatgcctc
     6301 tatcagtaaa tgaagtttcc tttaattctt ctactatata ttctttacca caaaacatta
     6361 ctttagaacc agctgattta gaagaagaga ataaaaatag attttctatt ggaaaatttc
     6421 tttcttctaa tacttttaaa aatgttctac ctaccattcc agtagcacca actattgcta
     6481 catttacttt tttcatataa attcacccca attttaaatt attaaaacat tattttattt
     6541 tagttataca agtaagatat gttaatattt ttatatatcc aaaaacttta atattatatt
     6601 aacaacttta attatataca aaaaacgtac aaaggtatac tttgtacgtc taatttatca
     6661 ctcaaagtaa accctgtagc tcatcaccta caaaaataag tgacagtatt acacatgtta
     6721 tgtgcaatcc cagaaaatat tattgcaaga aatattttct tcggctgtaa atcctttttc
     6781 caatcattaa cacctgccag ctactattgg atactcttag ttacagctac ctctacccta
     6841 ggttaatagg atattaaaat ttgattattc taataataat acatatttaa gaaaaaatca
     6901 acttatttat ataaatattg ctaaaattta aatccaaaat ttgtcaattc ttgttttaac
     6961 acattaagat tagtttcttc catttcagct agaggaagtc ttaaatcacc aacattaaaa
     7021 cctaaaatgt tcatggcagt tttaacagga actggattta cttctataaa taaagcagca
     7081 attaaagcat ccatatcaag ttgtaatttt tttgagcctt caatatcacc ttcaaagaat
     7141 ttagttacta aatcatgtgt ttctttagga catatgttag caagtacaga gataacacca
     7201 cttccaccta atgataatag tggtagtata gtatcatcat taccggagta tatagcaaag
     7261 tctttaggta ctaatttagc tatttctgca acttgagcta agtctccact tgcttccttc
     7321 acagcaacta tattatctat tttagcaagt tcagccacaa cagatggctt tatattcact
     7381 ttagttcttc ctggtacgtt gtataaaata attggcaact taacgctatt tgctattgtt
     7441 tcaaagtgaa gtttaagacc tttctcatta gtcttattat agtaaggagt tattattaaa
     7501 agaccatcca cccctaattt ttcagcttct tgactcatat gaacagacaa catagtattg
     7561 tttgaaccag tacctgctat aacaggtatt ctcttattaa ctttttcaac tgtatatttt
     7621 attacagcaa aaatttcaga ttcactcata gtagtcgcct cacctgttgt tccacaaaca
     7681 actagtgcat ctgtgccatt ctcaatgtga tattcgatta attcacctaa tttttcaaag
     7741 ttgacattat tatcctctgt aaatggggta actaaagcaa cagcagaacc tttaaataac
     7801 atataatcaa atctcctttt ttatctaaat ttaatttttt aagtacaata ataattatag
     7861 tacaaattaa ttaattttaa taggtatagt taatatctta ac
//
LOCUS       DAMJZE010000176         7827 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJZE010000176 DAMJZE010000000
VERSION     DAMJZE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.180"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..164)
                     /locus_tag="PC541_003216"
     CDS             complement(<1..164)
                     /locus_tag="PC541_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2840640.1"
                     /translation="MVIAMRVRIDFNPSFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQG"
     gene            complement(142..825)
                     /locus_tag="PC541_003217"
     CDS             complement(142..825)
                     /locus_tag="PC541_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840641.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKNKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDSNES"
     gene            complement(888..1566)
                     /locus_tag="PC541_003218"
                     /pseudo
     CDS             complement(888..1566)
                     /locus_tag="PC541_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(1819..2025)
                     /locus_tag="PC541_003219"
     CDS             complement(1819..2025)
                     /locus_tag="PC541_003219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840642.1"
                     /translation="MSNLREKIIKELNEFNIDADDEFLSYGVEYVESFIGKSAISEEL
                     LIRGVVFTANYIVNMKAKDGVNKA"
     gene            complement(2465..2947)
                     /locus_tag="PC541_003220"
     CDS             complement(2465..2947)
                     /locus_tag="PC541_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840643.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLESEFCGCKAQ
                     SYEEKTGETYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            complement(3051..3788)
                     /locus_tag="PC541_003221"
     CDS             complement(3051..3788)
                     /locus_tag="PC541_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDF2840644.1"
                     /translation="MNNLKLIENEGLIKVYTTDEDIKVVNGRELWEGLGVKQQFSDWI
                     ETNLKNVDANKNEFYILKGKTSKQGGRPTDEYIIKLEIAKEICLVAGASPRANKELKR
                     NSKNYRKYLIAVEEKYKVSNNLTKTQLNQINDIVSNALCEMQTKHDAQIEQFKKESSQ
                     YYRPTSKTKYDISSYIKNRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSLN
                     IIDESIRIIKSERKTNQISFFEKDNFC"
     gene            complement(3851..4258)
                     /locus_tag="PC541_003222"
     CDS             complement(3851..4258)
                     /locus_tag="PC541_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2840645.1"
                     /translation="MKVNFVIDGEPVGKERPRMNSITKRTYTPNKTKNYEELIKWLYQ
                     SKVKYRFTGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDIS"
     gene            complement(4248..4646)
                     /locus_tag="PC541_003223"
     CDS             complement(4248..4646)
                     /locus_tag="PC541_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840646.1"
                     /translation="MQKDIWLYSWDDEYFASDEYESKEEAINGAKEELKRFKEVKQCV
                     YIGKKEDVNIPNIDAEDALERVQDRIDDEFGGFGEDWFENIRAEDILTLENRISKVFK
                     KWIDEFGYKPYWFVVRDTEEIELNEVANES"
     gene            complement(4785..4982)
                     /locus_tag="PC541_003224"
     CDS             complement(4785..4982)
                     /locus_tag="PC541_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840647.1"
                     /translation="MKNKVEINNIQQLNDALNKYNIPFDVLSDIDRRICGWMATGGNE
                     DDAYIKQQYRYVENFINLFCD"
     gene            complement(5078..5407)
                     /locus_tag="PC541_003225"
     CDS             complement(5078..5407)
                     /locus_tag="PC541_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840648.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNNTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            complement(5489..6493)
                     /locus_tag="PC541_003226"
     CDS             complement(5489..6493)
                     /locus_tag="PC541_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2840649.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVTFYEEK
                     "
     gene            complement(6490..6711)
                     /locus_tag="PC541_003227"
     CDS             complement(6490..6711)
                     /locus_tag="PC541_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840650.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYYCDGGIEI"
     gene            complement(6729..7424)
                     /locus_tag="PC541_003228"
     CDS             complement(6729..7424)
                     /locus_tag="PC541_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840651.1"
                     /translation="MDLNKIMNDALVEIEESGFVEETVKKELEETIKRAVNKIFGSYS
                     KFRKNIEQHLGENININLDELDIQKYNLLVANVVKEKVDATMKGQGIEHLKRNLDNML
                     VGLKKEYKMSELLEELKEDKYNLDEYPCDEDCITFILESNFNSEWARWIDFDECPNVS
                     RNNCEHSILLRNDGTIAAFRYQNREISSKDIMNGFGNFGDLLFKIYAHNSKIILDLGN
                     DVDDYDLTNGEDY"
     gene            complement(7508..>7827)
                     /locus_tag="PC541_003229"
     CDS             complement(7508..>7827)
                     /locus_tag="PC541_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2840652.1"
                     /translation="NRNCDNSGVLVNAVLTPDRVNKRQNGRRIKESGEPMFTLTAQDK
                     HGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQLYKQAGNAVTANVVYEIAK
                     RMG"
BASE COUNT         2250 a         1528 c          736 g         3313 t
ORIGIN      
        1 ccttgatact ttttatctcc taactttagt atataatcct gtgctacatt aacagatttt
       61 ccactccctg ctgaaccttt cattgctctg tatctttttt tagtaaaatt agcttcttta
      121 aaagatggat taaaatctat tctaactctc attgctatca ccataatcta cacttatttt
      181 caactcatca tccccaatat catctttact taggttatca acctcacatt ttaacttctc
      241 aactctattt ttctgctcct ctgtagccaa attccaatcc ttatgaatca tttcatcata
      301 ctgcttaata agactcctta actcactcat tgccctactc tgtgcattaa gaaaagatgc
      361 ttgcctatcc catgcaaatt gaaattcata ctctatcttc tcaccatttt ctgtgctttc
      421 atgtttcttt aattccttaa tcatttcttc cttgtcttta acatacatta tcttctgtgc
      481 tcttattatt gctgcatatt gtattgttat ctgttcccaa agaatatcaa atttatcttt
      541 attctttatc tcattaatta agtcttgagt ttcttcaggt aagtattttg agaagaaacc
      601 aaacttttca gcatttttat taccaggtgg accagtagcg tttttattac ctatgggtgc
      661 acccctttta tttttaggtg cacccttctt tttctcacta gcccaattgt atctttttat
      721 ccatgacttt aaagtgttta aactaatgtc atactttgct gatatttcct tttgtttcat
      781 accttttatg taatcttgtt ttaccttttc tttgacatct tgcacatcac cacctcgttt
      841 gtttgtcgtt ttgggaatta aaaaagaccc tccatcaaga cagtcattta aatcatttct
      901 attaattctt taatcttttt atatacttct ttataattca tatctttatc tattaactta
      961 ggtaatttca tagatataat tctttcaagt gcttgtatat caaacagttc actttgattt
     1021 aactcatccc ttttcacacc ttttggtata cctaattttt ttcttacaag ttcggtaaaa
     1081 tgtttataat acatctgagg tttattactt ccttgactaa ttgcataata aacaaattct
     1141 tgtatttcat ctgtaaaatc ttttcttact ttttttacct tctgttctta tatccaacca
     1201 ttcttggtct ttttctgtag caatgtagta accatgtatt ctaatttgtt taagtgtttg
     1261 tgtaacccat tttgtaaata actttgcttc tggtttatta cttctaaatg acatattgta
     1321 cacagcttct tctgtaacaa aagtagtacc gaagttaggt aatttatctt taaagtttct
     1381 agtgtaggaa tctccgacag tagactcatt aaatttcttt ttatattctc tatctatatt
     1441 tcttaatgta tcacgaatat ttactatgcc tagttcctct cctacgtcat ttgcattaaa
     1501 ccaaacttct tctccatttt tggaccacat tacttttaca tttttctctt gtaaaatttt
     1561 caacatacta ctacctccta aattatttat attaataaag ggtgctcaat ttgaacatcc
     1621 tttttatata tcgacacctc aatttgaccc cccgtcattt cataaaatca acctcctggg
     1681 catttgaatt ttagatatgc atagtgtgca tatccgaata gtgcatatta tgcattttta
     1741 atagaccttt tcagatgtcg gagttaccga catctgaaac ttattaattt ttgtattaaa
     1801 aaagacctag aaattaattt aagccttatt tacaccatct tttgctttca tattcactat
     1861 ataatttgct gtaaacacta ctcctcttat caataactct tcactaatag cacttttccc
     1921 tataaaactc tcaacatatt caactccata acttaaaaat tcatcatctg catctatatt
     1981 aaattcattt agttctttta ttatcttttc tcttaaatta ctcatttatc atactcctgt
     2041 aaaaaaataa aagactaagc ttaccctcac ttagcctttt taaataggga gatacatata
     2101 ttatgtcgca agttctaaga atcgaactta gattaagcac cagcacctgc atggtgagtg
     2161 aggttaccaa gccccacccg atttttagac ttctgaatta agatacaaaa tataaaattt
     2221 tgccctcaat ttctctactt ttagcgtata cgttgattaa tatttgaaca tagttagaat
     2281 tgaactaaca acgtcctcac gccctgccta gtctgttcat attgctagat tagcccttta
     2341 aactaaccta gcaattattt agttttgaga gggaaatctt tatttccacg atattattat
     2401 ctcatgtttt tgccaacaaa aagtctcacg atagtctcaa aaaagtctca aaatagtctc
     2461 atttttaagc tttccatgaa aaaatgggta attcaaactc ttttattttt ggatataaca
     2521 tatccataat tttacaaact atcctttttc ttattctaaa gcaatgactt ctatctatgt
     2581 gcatagcatt agacatataa tccatgttga ttttctcatt gttcatatac atttcattga
     2641 agaactctgt ttcaaagcta tttagacttg ttaatgcaca ttctatagtt tctttttcaa
     2701 tttctaatgt tttcttatct tcttttaatc tatttaaatc ttcttctctc ttaataactt
     2761 cattttcaac acttgaactt atattataag tttctccagt tttttcttca taactttgag
     2821 ccttacatcc gcaaaactca ctttctaatt ttttaatata tatatctttt attcttattt
     2881 gactttctag ttttttatag ttatacaatc taccttcaac ctcttgaaat agtgtctttt
     2941 tattcatact tccacactcc tatcaattat ttatgttata ataatatttg tatataaaag
     3001 ttttatattt ttgacaactg gagtgtgaaa gcactccttt tttcttttta ttaacagaaa
     3061 ttatcttttt caaagaaact aatctgattt gtctttcttt cagactttat aattctaatt
     3121 gattcatcta taatgtttag ggattttaat aacacatctt ttggtatgtc ttcccactta
     3181 tcagctccaa gaattaataa agttctcttt ttaactaaat caaactcctc attagctcta
     3241 ggaatatcta atctattttt tatgtagctt gaaatatcat acttagtttt acttgtaggt
     3301 ctataatatt gtgaggattc ctttttaaat tgttctattt gtgcatcatg tttggtttgc
     3361 atttcacata atgcgttact tacaatatca ttaatttgat ttaactgtgt ttttgtaaga
     3421 ttgtttgata ctttgtattt ttcttctact gcaattagat attttctata atttttagag
     3481 tttcttttaa gttctttatt tgctcttgga cttgctcctg ctactaaaca aatttctttt
     3541 gctatttcta atttaattat atattcgtct gtaggtcttc caccctgttt agaggtttta
     3601 ccttttaaaa tgtaaaactc atttttatta gcatccacat tttttaagtt tgtttctatc
     3661 cagtctgaaa attgttgttt tactcctaat ccttcccaaa gttctcttcc atttacaact
     3721 tttatatctt catctgttgt atatactttt ataagtcctt cattctcgat taattttaaa
     3781 ttattcatac ttataatcct cctccaaatt atttttatct aatgatttga taaatggatt
     3841 tttccattgt tcaactaata tcttctaata taacctcaac cctgggattg tcactataat
     3901 atttactagc tacaacctca acaatctgtg tatcatcctt ataagctatc tcattgagtg
     3961 aatcagctat aatcttaaca acattgtcta tatcgggttt cttactaggt cttaatacat
     4021 tatttctttt ctgctcttta acctttttac tgttactttt agctatagaa tagtaacatc
     4081 ttaaagtcat ttttatataa ccagtaaaac gatacttaac tttagattga tacagccatt
     4141 ttattaactc ttcataattc ttagtcttat taggtgtata ggtcctttta gttatagagt
     4201 tcattctcgg tctttctttg ccaactggct ctccatctat tacaaaatta actttcattt
     4261 gctacctcat ttagttctat ttcttccgta tctctaacaa caaaccaata tggtttatat
     4321 ccaaattcat ctatccattt tttaaatact ttgcttattc tattctctag tgttaatatg
     4381 tcttcagcac gtatattttc aaaccaatct tctccaaacc ctccaaattc atcatcaatt
     4441 ctatcttgaa cacgttctaa agcatcctct gcatctatat taggtatatt aacatcttct
     4501 ttttttccaa tataaacaca ttgtttaacc tctttaaacc ttttaagttc ttccttagct
     4561 ccattaatag cttcctcttt gctttcatat tcatcacttg caaaatattc atcatcccaa
     4621 ctatataacc aaatatcttt ttgcatatta atacctccac tatttatttt tcttattagc
     4681 cttctttcta cagtccttac aacaataaat ctccttagat ttttccttaa gataaaacag
     4741 cttaccacac caactgcatc ttcttcgttt catagaatca cttcctagtc acaaaacaaa
     4801 tttataaaat tctctacata tctatattgt tgtttaatat aagcatcatc ttcattacca
     4861 ccagtagcca tccagccaca tattcttcta tctatatcac ttaacacgtc aaatgggata
     4921 ttatacttat ttaaagcatc atttaattgt tgtatattat ttatctcaac cttatttttc
     4981 atatgcaaat cacccttttt ataagtcaaa gtaagtctat aaacttctag tttcattcac
     5041 aaacttacct tgactattta tactccattt ttattattta atatttagga aattctccat
     5101 aagtcgtagc aagacattca attctccact ctggtataac ccatgttgta ttgttatcac
     5161 ctacatatgc ttttacttta taaagcgggg tatttctttt tctaaatttc gtactataac
     5221 atttttctaa aatttcacaa ccaaccattc tgttatagta acagtttatt tgatgatata
     5281 taatcttttc ccctacagaa tatttatatt ttaaattatt tatagatttt ttatatatgc
     5341 gattttttac ataatttgct aatatacaaa taattattag cagtatagtc aataaaaaaa
     5401 tcttcataag ttattcctcc taactagttt aaatttacat cctcttatta tttacttctc
     5461 cttctctagc caatagtcac atgagtacct acttttcctc ataaaatgtt acattcttaa
     5521 taattatatc tatagaccca ttttgatttt gtcttacagt atacttcatt gggtcttcaa
     5581 aatcagttag attccctttt atgtcaaaac cattatccgt ttttatactt ctttttttaa
     5641 gctttttctc aacccatttt ttatctatac taaaactttc gtcaagacct ttttcttcca
     5701 tatgctcttt aaagctatcc tttaactcat catctttaat tgaattatca acaaagtcat
     5761 ttatatcaac ttcatgtttt tccttcagtg tataatttaa tatacttctt acatcttcag
     5821 cttgctttat atcattacta agagcattgg ttatccaatt ttcagctgta tttttaaata
     5881 ccttggtctt gtacttatca tcatctattt ttttagcatt taaaaattct gttacaaact
     5941 tagattctga cccttctttt tcagcatcct tatctaaaag ccttagatga aactcatcat
     6001 ttataccact tagactaatc aaagctcctt gcttttgtct taaagtctct gggataccaa
     6061 tttcatttga aaccatttga atattaaatt tatcatccac aaattcaatt gaatgagtgt
     6121 acaacttttt ataatctaat ttcagtattg ctacactctt ttcatcttta atagtgtata
     6181 aacaaattgc caaatcacaa gattctagtg cagcattaat tttcataacg tcaaacaagt
     6241 aagatgcaat ttctttagaa tttttcaaaa aagtactttc atcataaata atttgttcac
     6301 aacacttctt aattagattg ttactatagt cattaaatac tgctgttctg atgtcattat
     6361 ctcttgatac tttgcttatt ttcttttgaa agaaagcttc aatatcttga ctaaccctac
     6421 cctcaaaatc atttagtatt ggtgtatcgc tattcttatc taaaacatgt attataaatt
     6481 tgtgtattat catatttcaa ttcctccatc acaataatat tcagccattt tttgacttct
     6541 agtatctttt ataacttctt caactttatc tattgttaca agcagtatct tgtcatcttt
     6601 agctaatagt tctgcttttt gttttaacat ttctttactt ccacatgaat aatttatctt
     6661 gcgttcatct agtaatagtc ctttttgcca tctcaataca tactttgaca tttgttccat
     6721 cccctatttt aataatcttc tccatttgtt aagtcataat cgtctacatc attccctaaa
     6781 tctaagataa tttttgaatt atgtgcatat atcttaaata ataaatcccc aaaattacca
     6841 aatccgttca ttatatcttt tgatgatatt tctctgtttt ggtatctaaa tgctgcaata
     6901 gttccatcgt ttcttaatag tatagaatgt tcacaattat ttctgcttac attaggacac
     6961 tcatcaaagt ctatccatct tgcccattct gaattaaaat tagattcaag gataaatgtt
     7021 atacagtctt catcacatgg gtactcgtca aggttgtatt tatcttcttt caattcttct
     7081 aataattcac tcattttgta ttctttctta agacctacaa gcatgttatc caagtttctt
     7141 tttaaatgct caataccttg ccccttcatc gtagcatcta ccttttcttt tacaacattt
     7201 gccacaagta aattgtattt ttgtatatct aattcatcta aatttatatt tatattttca
     7261 cctaaatgtt gttcaatatt ttttctgaat ttactatagc ttccaaagat tttattcaca
     7321 gctcttttta ttgtttcttc taattctttt ttaactgttt cctctacaaa gccactttct
     7381 tctatttcca ctagtgcatc attcataatt ttatttaaat ccattttatt atcccacccc
     7441 ttattttcat ttttgagagt tacaaaacac ttcaaaaata ttcatactaa aagacatttt
     7501 gcaactttta gcccattctt tttgctattt catatacaac atttgcagta acagcatttc
     7561 ctgcttgctt gtacagttga ctatctgagc atacacttgc tgctctttcg taatatttat
     7621 ccggaaatcc ttgcaaccta aagcattcct ttggtgttaa ccttcttata tctccatttt
     7681 tcaaaattcc atgtttatct tgagctgtca atgtgaacat tggttctccg ctttctttaa
     7741 ttctacgacc attttgtctt ttatttaccc tatcgggcgt taaaactgca ttaactaaaa
     7801 ctccactatt atcacaattt ctatttg
//
LOCUS       DAMJZE010000177         7752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJZE010000177 DAMJZE010000000
VERSION     DAMJZE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.181"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(318..980)
                     /locus_tag="PC541_003230"
                     /pseudo
     CDS             complement(318..980)
                     /locus_tag="PC541_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1140..1676)
                     /locus_tag="PC541_003231"
     CDS             complement(1140..1676)
                     /locus_tag="PC541_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2840653.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1749..2162)
                     /locus_tag="PC541_003232"
     CDS             complement(1749..2162)
                     /locus_tag="PC541_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2840654.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2628..3182
                     /locus_tag="PC541_003233"
     CDS             2628..3182
                     /locus_tag="PC541_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2840655.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3790..3969)
                     /locus_tag="PC541_003234"
     CDS             complement(3790..3969)
                     /locus_tag="PC541_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2840656.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4195..4728)
                     /locus_tag="PC541_003235"
     CDS             complement(4195..4728)
                     /locus_tag="PC541_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2840657.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            5045..5395
                     /locus_tag="PC541_003236"
                     /pseudo
     CDS             5045..5395
                     /locus_tag="PC541_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6476..7048)
                     /locus_tag="PC541_003237"
     CDS             complement(6476..7048)
                     /locus_tag="PC541_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2840658.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(7110..7373)
                     /locus_tag="PC541_003238"
     CDS             complement(7110..7373)
                     /locus_tag="PC541_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2840659.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7373..7594)
                     /locus_tag="PC541_003239"
     CDS             complement(7373..7594)
                     /locus_tag="PC541_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2840660.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2680 a         1330 c          927 g         2815 t
ORIGIN      
        1 atagcttgta gtgtacttcc ctcaagagga atagtcgttg aagtttctcc tattcggagt
       61 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
      121 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      181 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      241 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      301 ttcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      361 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      421 ttatggactt cttcaagtga aaacataaca cccgctagtg gtgcatgaaa ggctgctgat
      481 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      541 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      601 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      661 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      721 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      781 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      841 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      901 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      961 tgagatgtat cctttttcat actattcctc cttcatccaa taattctaca caatctgaat
     1021 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1081 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
     1141 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattaa ttcttgcttt
     1201 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1261 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1321 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1381 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1441 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1501 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1561 tcaatagcag tcttatccca tatacttttt aataatataa tgacatcttt tgcactttta
     1621 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1681 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaatag
     1741 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1801 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1861 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1921 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1981 aacccttttt gacagacaca atcctttatt ttgcacttac ggcagtgttc tggctttcct
     2041 gtatcgccgt tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2101 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2161 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2221 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2281 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2341 acacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2401 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2461 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2521 gttttgaata gtagataatc atatgattaa aaattaaata tgacatagcg tttgtggaat
     2581 ataacaccta aattatgtta taataataaa gcaggagtgt gatagtgatg gatgaaaaaa
     2641 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
     2701 gtatcgaagg cactattagt gatatgcaaa gcaatattaa tggtatcgaa gacactatta
     2761 gtggcatgca aggcactatt agcgatatgc aaggcagtat cagtagtatg aatggcacta
     2821 ttagcgatat gcaaggcagt atcagtagta tggatggcac tattaatagt atgcaagaca
     2881 gtattactga tatgcaagtt gaaattaaag aagtcaatag taggttagac aatatggaac
     2941 ctgatattaa agacttaaaa actggacaag ttgaaactag tagtagactt gatactattc
     3001 agagtgatgt tacagaaatt aagtcagatg taaaaattaa cagaaataca ttagatacag
     3061 tgattaaaca tacctcttca ttagtaaaag atacatcaga tattaaaaat gatttaaata
     3121 atatagagat agtcacatct aagaattcga atgatattgc taaattaaaa tcagttaaat
     3181 aatatactat agtaaaaaat acctgaaaat ttcacatact tctttaaagt tataccaaat
     3241 tttatacaga aatactgatt atccgacaac acactttact tataaataat ttattaaaaa
     3301 aatactcatt ttatcctaaa attaacacga ctaaatgagt cttgatataa taataaaaaa
     3361 taagggagat tgaacactaa aaagttgtgt atctccctta tattctataa tatagttata
     3421 taatttttca gcctaaaata aaagtccatc tttgattaaa acacatcccc ttatcactta
     3481 taaaaggata aaatcttgtt ttcatttttc catttatatc ttcttgctta agagtaataa
     3541 ttttttcttt tgatacttta agtgattggt taaatttaac caaacgacta agttttgttc
     3601 aacatctatt tttcatattt tccaatactt aatctatttt ttataaataa ttcttttatt
     3661 tctaactcac ttacattttt tacataaaac gaagcatata aatttcaacc ttatcttgta
     3721 tggttcttaa tacttaatga gtagcttaag tatcaatcat tataaatttt tcccttacat
     3781 ttaacactct taccatttcc aaaatataca aaatatattt tgaacatttg tacaatgatg
     3841 aaatacaata catgaaaata caatatcata actgttgtct aaaaatagaa gttcttcaac
     3901 ataacctttc acaaaaaaca tattatctaa gtattttttg tctgctcctt gttttcctgt
     3961 ttgtagcata taaattactg catctaaaca tataactact cacacgaaaa aagaaaataa
     4021 acttccaaaa tacaagctct aaaaactacc tctaaagttg tataatgttt actaggaata
     4081 aggttgtata acatatagtt taaataatca ttttttaaaa ttgcaagtta ttgctttcaa
     4141 aattagatgt ttttatatca aataaatgat taagaatttc taaatttaat ggctttataa
     4201 tgcactccct ttctttggga taaaataatg ttgcacagat actttttcta aagataacaa
     4261 ttttattttt ctatcaattc caccagcata cccagtcaaa ctcccatttg ttcctacaac
     4321 acgatgacat ggaataataa tagagattcc attatgccct actgcaccac cgattgcttg
     4381 tgctgacatt ttagatattc ctttttgttg agcaacttta tatgcaattt ctccataagt
     4441 gattgtttta ccatatggta tttgttgtaa aattttccac actgtagtct gaaaaggagt
     4501 acctaccata tggagtgatg gtgtaaaatt gggttctttt cccgaaaaat aaatatctaa
     4561 ccatttcttt gtctgctcga aaattggcaa ttctttttct tgatgttctt ggtcaagatt
     4621 ataagcataa tacttttgat tctcaaacca aagaccagtt aacccaatat tatctgatgc
     4681 taaaagaata tttccaattg gactatggta atagctaata tattgcatac aattcctcct
     4741 tatattctga aaacttcact caaatcatta gacacttctt ttctatctac cattatgtaa
     4801 aaacatacta atataatttc cttctaacta cactttcgtt ataatatcta tcctcatatc
     4861 atattagttt tatatttacc tgcatctgtt catccttttt tccctttccc tttgatgaat
     4921 ataaaattca tacatttaag cttcctactg tattaacttt tatagaagtg tatattataa
     4981 ttttatcact aaataggcca aaaaaatatc gatatagcat caagtaatat cataatactt
     5041 tcaaatggga aaagtaagaa taggctgtaa aaaagaaaag tatttttatg ttagaaatcc
     5101 agtaaaatca ttagtagata gaataacaga aaacgaacaa tttgctattt atcattttac
     5161 acataaatat aattgacgca ataaagtgtt ttctattcta caacgattaa taaggttaga
     5221 aaaaagtaaa aatgaactgt atatgtatga agcattagtt tctgtttctt tcctttggct
     5281 tattatgata aaaaatattg ttttgctacc aataaaacac aaaaatataa ttaatatcca
     5341 tatgcaaaaa attttacgtt atattgaaaa atactattgt tttacattat attgaagggt
     5401 actattgtga aaacttaatt ttggagtact atcaagcagt ggtaatatca ataaatcaga
     5461 gtgctccaga tgatttgaac taagtttaaa tactatacct tataaatatt ttactgagtt
     5521 ttgtttatta aaagcaactc aatttctaaa aggcacaaat gagctaataa gtgacattgc
     5581 tataaaagtt gtttttcatc aaataatcta ttttagtaaa tgttttaaag aaaaaacggg
     5641 atattctcca aaagtgtaca gaaatataaa aaaagagata tgatttttta tttcttttga
     5701 ttatccaaag agtaaataaa aaatattgta aaaaacttac taacaacggc aatgctttga
     5761 actatggtaa tactttgaac ttttgattgg actttttttg ctaaaataat atatttaaat
     5821 agcaaagtaa tctgtttaat caataaaaaa taagagataa aagaaatcaa ctaaaacttt
     5881 tacctcttat tttgatagtt tatcagagct aatatttaca ctctttttat ttttgatact
     5941 tcattaaaaa tgatgcttac tctccattca cttttaattc ttcattaaaa atgatgctta
     6001 ttctccattc acttttaatt cttcattaaa aatgatgctt actctccatt cgcttttaat
     6061 tcttcattaa aaatgatgct tattctccat tcacttttaa ttcttcatta aaaatgatgc
     6121 ttactctcca ttcgctttta attcttcatt agaactatgc ttactctcca ttcactttta
     6181 attcttcatt aaaaatgatg cttactctct gttcactttt aattcttcat taaaaatgat
     6241 gcttattctc cattcacttt taattcttca ttaaaaatga tgcttattct ccattcactt
     6301 ttgattcttc attaaaaatg atgcttattc tccattcact tttaattctt cattaaaaat
     6361 gatgcttatt ctccattcac ttttgattct tcattaaaaa tgatgcttat tctctgttca
     6421 cttttaattc tttattaaaa ctgatgctta ttctccattc actttcaatt cttcattaaa
     6481 actgatgctt gcactctgta aactcataaa catccattaa tgcttcacca aatctttgat
     6541 agtgaactac ttccctctca cccaaaaatc tcaatatatc tttcaaatca gggtcatcag
     6601 ttaaatttat taattgatag tacgtagcca atgctttttg ttcagcagcc atatcctcat
     6661 gtaaatcagt taccgaatcg ccaaagacat taatataact tgtagtaaag tttactccat
     6721 tagaatctac tggctgaaga ccatgtccaa aattagcata attactacca agtccagcag
     6781 ccttaagctc ttctggagta caattgccta ttaattgata taacactgaa gaaattatct
     6841 caacatgagc catttcctct gtacctatat cagttaaaag tgcacgagat tttccagttg
     6901 gcatagtata tctttgttga agatatctag ttgcagctgc caactcacca ttaggtcctc
     6961 ccaactgagt catgatatat tttgccattc tagggtctgc ttgtcttatg ttaactggat
     7021 gttccagtgt tttttgataa atccacatat tatcttactc ctccttaata tatatttaat
     7081 aataaatcta attttaaagt ttaataattt tatttataaa atgatttgtc ccatggccaa
     7141 ggttcagata tccatgacca aggatattca cttgattcat atccaaaatt agttaatgct
     7201 ccatatttag attcataatc acacacagct tgtgtaaatt tatcgcagta tctgttgtat
     7261 gaatcaagag ctttcttatc ccttggattg ttatctaaat ataaatttaa ttctatacag
     7321 gcaaattgat attctgaaat tctctctaaa agttctcttc tatttaatga cattagtaca
     7381 tgttaccccc ttttttagct ctaactctat ttttaggatt tgcatatagc caatcattat
     7441 agttatttga ctctattaaa tctaattcag gaaataaagt acctcttttt aatgctgttt
     7501 ttggattgta tgtgtcatca taaatttgac gattgatgta tggtctagca aattcacaac
     7561 aatagtcttg tttatcctct tttaaatatc ccattaatta tcctccttaa tttttagaat
     7621 tttaaaatag cattattttt aaaaattgta tactggttct atattataac tggtagtttt
     7681 aataggtctt taataagatt aaatgattaa ataagtatct ttttttctta ttttacctct
     7741 aaaatttttc ta
//
LOCUS       DAMJZE010000178         7743 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJZE010000178 DAMJZE010000000
VERSION     DAMJZE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.182"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            82..414
                     /locus_tag="PC541_003240"
     CDS             82..414
                     /locus_tag="PC541_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2840661.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            576..761
                     /locus_tag="PC541_003241"
     CDS             576..761
                     /locus_tag="PC541_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840662.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            788..1873
                     /locus_tag="PC541_003242"
     CDS             788..1873
                     /locus_tag="PC541_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2840663.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            1866..2324
                     /locus_tag="PC541_003243"
     CDS             1866..2324
                     /locus_tag="PC541_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840664.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2359..3450
                     /locus_tag="PC541_003244"
     CDS             2359..3450
                     /locus_tag="PC541_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2840665.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3455..3679
                     /locus_tag="PC541_003245"
     CDS             3455..3679
                     /locus_tag="PC541_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840666.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3689..4252
                     /locus_tag="PC541_003246"
     CDS             3689..4252
                     /locus_tag="PC541_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840667.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4213..4842
                     /locus_tag="PC541_003247"
     CDS             4213..4842
                     /locus_tag="PC541_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840668.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4882..5019
                     /locus_tag="PC541_003248"
     CDS             4882..5019
                     /locus_tag="PC541_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2840669.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5019..5771
                     /locus_tag="PC541_003249"
     CDS             5019..5771
                     /locus_tag="PC541_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840670.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5784..6269
                     /locus_tag="PC541_003250"
     CDS             5784..6269
                     /locus_tag="PC541_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840671.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6276..6425
                     /locus_tag="PC541_003251"
     CDS             6276..6425
                     /locus_tag="PC541_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840672.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6561..7382)
                     /gene="yidA"
                     /locus_tag="PC541_003252"
     CDS             complement(6561..7382)
                     /gene="yidA"
                     /locus_tag="PC541_003252"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2840673.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT         2979 a          854 c         1362 g         2548 t
ORIGIN      
        1 tacagataaa taagaataaa cttataatat aattgtatct gtaaaatttt gtaaaatata
       61 aaaagtatag ggggaataaa aatgaaagta gcttatattt ttagtagtac taatacacat
      121 aaaattctag acaaaatgat aataccacaa cttgaacaag gaactcatgg agtagacgtt
      181 ctagggatgt ttttctttat ggataatacc ttatttttat ctaaaggtaa tcctgtaggc
      241 gagagactaa gtaaattaca tgaaaaaact gatatgataa taatggcttg tgaccaatgt
      301 gctatagaaa gaggtataga gaataatttg gttgatggag ctacaattgg atgtttccca
      361 aacctatatg cttgtttagg cagtgcaggt gtggaccaag ttataacgtt ataaattagt
      421 ataggataca atccatagaa aaatttattt tctgtggatt attttaagta aaagataaat
      481 tgtttaaagt aggggattaa caaatgtcta aaattttagt tgaatttata aacacttgac
      541 catcttgtga tgaacatgga gtgtttataa aaaaaatgtc agaaaaatat gctgataaat
      601 tggaaataaa attatatcaa gcagggaaag atttttcgta tataaaaaaa tatgggatta
      661 taacaaaagg aactttaata ataaatcaga aaaagaaata tgatagatta aataaagata
      721 caattgaaag ggcaatagta gaagctataa acaataatta aaagtttaaa aattaggagt
      781 atcaaaaatg aatatattag aaattgtttt aaatggagtt aaagatacta tagtagcttc
      841 tatagttaca ctgaatggag catcaggatg gcttatgata agttttatat tggcaggtgt
      901 acttcacaat gttttaactc caagaaggtt tcaaaaacaa cttggaaata agaaattttc
      961 ttctttaatt aaaagtacta tctcaggaat gtttttacca atatgtagtt gtggagtaat
     1021 accacttgga ataagtatgt attattcagg agcatattta ggaccagtac ttgcttttat
     1081 gacatcaact ccaataataa atccaatagc tgtagttctt tgtctaggtc ttttaggtcc
     1141 taaagttaca atcatttatc taatagctgg ttttttagta ccatttttag taggattttt
     1201 aggaaattgt tttggaaaag aagaattatc acttcataat gattcagagg aagaagtaat
     1261 agaattagaa gaagaaaaaa gaagttttat gggaaatatg attgatggtc ttaaatggtc
     1321 ttttggtgaa ttagccctta ctattagtaa atatgtagta atgggtatgg tttttgcagg
     1381 atttataaca actatttttc caaatagtat aattcaaaaa tatttaggaa atccaggttt
     1441 gctatctctt ggaagtatag caatacttgc atgtgttatg tatgtatgtg ctgtaggaca
     1501 tataccgttt atagcagccc ttgttgcaag tggagcatct cctggtatag cgataacatt
     1561 tctaatagct ggagcagcta caaatctacc agaacttata agtatgttta aacttattgg
     1621 aaaaagaact gttattatat attctgtaac tttgacactg tcttcaataa taattggata
     1681 tataacaaat ttacttttag tgaataataa ctcagtaaat ttagatgcaa caaattcttc
     1741 aatacagttg gcaaataaac taatgttaaa tatacctgat agttttgagt atgtatgtac
     1801 tattttaatt attttatttg catttaaatc aataatacca aagatgaaag aggtcatagg
     1861 aaatcatgct taaaaagata gttataatat cctgtatagt agttgtgtta atcattttat
     1921 caaaaatagt tgatgataac atcaaagaag atgcttctat tccaaatgtg aataaagaaa
     1981 cattagaata ttttagaaaa aattataaag aagacataat aacttgtgct gaagaagact
     2041 taaataatga tggaaaaaaa gatttagtag ttatatataa aaaaagcaat aattcaaatg
     2101 aaatggtagt cgtagtttct gataaaaata gccactatat aacaaagcca ataccagcac
     2161 ctatagagaa tcaaacgata acctttaaaa atattgatga taaagcacca atagaagtaa
     2221 ttgtatcagg gtctaaaaat ggtaatgtag gatatgctat ttacagagta gagggtaaaa
     2281 aatttgttga tttatttggc gaagatatgg acaaatgttg ctaaaaataa aaataataca
     2341 taaaggtggg aaatttatat gaaattatct aagaagattc aagcattaat cttatttggt
     2401 gtaatgggta catctatatt aacaggatgt agtagtaaac caaaagaaaa agaagaagca
     2461 aaaactggaa aagatgatta tacaataaca gtaggttatt ataattgtga ccatatgact
     2521 gcgggaccag tagcagaatc ggcaggaata tataaagatt taggtcttaa tgtaaaaact
     2581 gttggaaatg gtaaagttcc tgaagctatg gcagcaggaa aaatggatgc aggttacata
     2641 ggaactaagg ggttagttgg agcaatacct aagggttcgc caataacaat agcagctaac
     2701 aaccatactg gtggttctga atatttaata gtttcaaaag atataaaaga acctaaagat
     2761 ttaataggta aaaaaattgc aacagatatg agtgatttct tatggacaag tgattatggt
     2821 ccagaaacag gtctaccaac tgacccatca aaatatgaag ttgtaaatat ggattcagac
     2881 aaagataaat atttagctct taaaactggg aaaattcaag cttttacatc ttgtgaccct
     2941 tggggttctg tagctgaaaa tgacggtgca ggaaaaataa tagcatctac tcaatataaa
     3001 gaaaaagcta atggcaaaga atataactgt tgttcattct cattaaacaa aaactttata
     3061 aaagaacatc cagatttagc aaaaaaattg gttctagcac atacaaaagc aatagaatat
     3121 atatatacaa atccagctga agcagctaaa atatttgcaa aatattataa tgtagaagaa
     3181 gaagttgcac ttagaactat ctataagaag actgttggag aaggtagaac tcttacttgg
     3241 aaagttacag gtgaagaata taaaaataat ttacaaatgt ataaagattt aaaggcttta
     3301 gatgatatac ctaaatatga agatacgatt gatacaagct tattggactc ttgtggagct
     3361 gatgattttg acaaatttat aaaagaaaaa gttaacccag agtttcctga aggtatgagt
     3421 tatgaagact ggaaagcaaa ggtattataa ataaatggag aatatgaaaa atataataat
     3481 actttttatc attattttgg gaagtatatt tataatattt tttaattttg aagataaaga
     3541 agaaataaaa ggaaaattaa gaataggggt atctgatgat acctctggtt ttgtaataaa
     3601 ttatatggta aatagagact actttgaaaa tataaaagta agtgatataa tggatatttt
     3661 tacaattaat gattgctgag caagtacaat gcagtgggct ctgagttcag aggaaataga
     3721 tatagcaatc atatgtaaag atgctgcaaa acaatatgta aatgtaaata gtggatttga
     3781 aattgttggt actgtagttc aaaattctga tatatttcta ttaagtgata ataatcctga
     3841 aaagatagga gtcattcaaa atagagatta ccagtcagaa ttggttaaaa aatactacaa
     3901 gaatgtggaa gtagctccct tattagggac tgcactagca tatggattag aaagtaattt
     3961 agtagatggt gttgtaatag attgcataaa gtcaattggg ttagagggta aaagaaaatc
     4021 tacaactaaa ttaggagatt atgataccta tgtattagtt gtaaataaag agtttaaaaa
     4081 aagtaaagca tatagtgatt ttataacact ttacaataaa agtgttgatg ctttaaagaa
     4141 taaagctaca tttaagaaag tattaaaaga gtacaggaat ataagaaata tagatatgga
     4201 ggaaataagc aattggaaat taaaatttct aaagataaaa gcgatagatt aactaagaaa
     4261 aaaactgatg gtagtatgga tattttatat aaaataatac tattagttat tatattttta
     4321 gtctggcatt ttgcagcaaa agatataggg agttctttgt tacttccaat gccagtagat
     4381 gtaataaaag gtttcttttt ctgtgtgaca gatgcagaga cagtcactaa tctttttata
     4441 acattgcaga gagtacttaa aggttttatg tatgcattat tgtttgggct tccaatagga
     4501 tttattatgg gattttctaa aacttttgaa agggttttat ctccagtagt agattctgta
     4561 aggcaagttc caataatggc atgggttcct cttactatag tatggtttgg tataggagat
     4621 ggtccaacaa tatttttaat agctttttca ggggttttcc ctataatttt aaatactatt
     4681 caaggagtaa gagcaatatc aaaggattat tataatgcag ctagaagtat gggagcatcg
     4741 ccaattgtga tatttacaaa tgtaattgtt cccgcatctc ttccagatat actaactggt
     4801 tcaaggattg ctataagtac aggatggatg tctgttatct gagccgagtt tatagctacg
     4861 agtgccggtc tcggttactc aatggtacaa gctcaaacat taatgcatac agatgtatta
     4921 attgggctta tgatatttgc agctttaata ggatttataa tagatagggt attaaagttt
     4981 ataaataaaa tcctttgtag atggaggttt gcagattaat gaagttaagt gtaagagata
     5041 taaataagac atttgtaaat aatagagtac atacaaaggt tttagaagat ataagtatgg
     5101 atatagatga tggacaattt gtctgtcttt taggaccatc aggttgtgga aaaacaactt
     5161 tacttactat aataggtggg tttcaaaaat cagaacgtgg agatgttttt ataaatgata
     5221 aaagagttaa aaaaccgggc atagatagag cttttatatt tcaaaattat gctttatttc
     5281 cttggaaaac tataagaggt aatgttctat ttcctatgaa acagcagaaa ataccaaaag
     5341 aaaaaagaga agaaatgcta gaagaacttt tggttatgtc agacttgaaa ggaaaagaaa
     5401 aactgtttcc gcatcaatta tctggaggaa tgaaacaaag agtggccatg ataagagctt
     5461 tagcatgtaa tccagaggta ttactaatgg atgaaccatt aggagctgta gattttcaaa
     5521 tgagacaaaa tttacaggaa gagctagaac gaatatggat aaagaagaaa ataactgcat
     5581 taatggtaac tcatgatgtt gatgaagctg tatatatgag tgatagagtt atagtaatgt
     5641 cgagagataa aggaagaatt attgatgata taaatataga tattccaaga cctagaatta
     5701 gaggaagcca aaaatatgaa gagtacaaaa acaaattaac tgatacttta tcaaagtgtt
     5761 atgaagttta aagaggagta gacatgagtc aaatagaaaa atacaatagt ttggataata
     5821 cacaacagaa aataagaatc tctattatgg atatgataat agataaaggt tctagtgtta
     5881 cacttcaaga agtgactgaa tatgtaagta aaaagttgaa tatagaaaaa gaatatatag
     5941 aaaggacact acaatatttt atttataaaa atataatggt tgttgatggg aatagtataa
     6001 attttatata tcctgtatca gcattgccaa caaatcataa agtaactttg agagataata
     6061 gaagtttcag tgcaatgtgt gctatagatg ctataggaac ctcttgcaca ttcaaccaag
     6121 atataaaaat aaactctatt tgtagtgcta caggtaagga aatagaagta attataaaaa
     6181 atgaaaagat tgagtatgtg aataatccaa acttaagagt gcttcatata aatcttgata
     6241 agcatctaaa ttgggctgct agttgctgaa atataatgaa tttcttctgg acgaagagtg
     6301 attttgatgc ttggacaaac gaagcgggat tatctgatga tgaagatata tattgtttag
     6361 atattaatga ggcaatagta gaatcatata aaatatttaa actcaaacaa aaggtactat
     6421 catagatata catatatgaa aaactaaaaa tgacctttag ataaaaatga taggtaatat
     6481 ccatactata taaattagta tagagtaggg atgtgaaaag tatttaaatc taaaggtata
     6541 tttttagttt ttttagatga ttagatagcc ttattaagta tatatttatt tataaccttt
     6601 gcaactccat tttcattatt agtatcagta acataatctg caatttcctt tatactatta
     6661 cgtgcattcc ccatagccac accaagacct gcatacttta tcatatgttg gtcattttct
     6721 tcatcgccaa cagcaatagc attttctata ggtatattta gattcgtaca taatgtttta
     6781 agagcagttc ctttattagt agttttacct aaaaattcta gataaaaagg agcagacctt
     6841 actatagtat atttattgaa taaattttta ggtagttgct gtatgatttc ttccaatctt
     6901 tctggttcat caactatcat aattttacag attttaatag attcatcaat tttatctttt
     6961 tgtatatatt tgacttctat attatttaat ttcgcttcat gaagtgtata tttgccaggc
     7021 gtttcatttg gtgtaattaa atttgtcatt gtatttatgt gtattttagt cttatatttt
     7081 ttacaaacgt tattatatat aaaatcaaaa tcatctaaac tcatttctac atcatgtatg
     7141 attttctcac ttttaacttc ttgaataatt gctccattaa agcatataac ataatcacct
     7201 tcatctgtta aatttagttg atttagaagt tcttttacac caggaagagg tctgcctgtt
     7261 gttataacta cttttgcccc agcccttttt gcctcctgaa tggactcaaa gacttcagga
     7321 gtaatccttt tttgtgtatt taatattgtt ccatcaatgt ctagtgcgat taatttgtac
     7381 atatcaacct ccaataaatt atatactcaa atttcctgtg aaaacgttga cacagctagt
     7441 gttgaacaaa gtttcagtgg gaaatatggt ttgttttgta tactattata tcaagagata
     7501 agtaatttag aaagtttaat aatattaatt ttttgataaa aatattactt atgtgagatg
     7561 agaatgtaaa aactttgtaa tcaataattc caaaattctt aattacaaat aatggtatcg
     7621 tttgcattgt taatataaat aaatgtatat tatatagtta gaagtttgaa taaatagact
     7681 gatggtaatt tgttatatta gttagttttg tgttataaaa tattgtaatt ccattaatca
     7741 tat
//
LOCUS       DAMJZE010000179         7715 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJZE010000179 DAMJZE010000000
VERSION     DAMJZE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.183"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            322..1623
                     /locus_tag="PC541_003253"
     CDS             322..1623
                     /locus_tag="PC541_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2840674.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1699..1974
                     /locus_tag="PC541_003254"
     CDS             1699..1974
                     /locus_tag="PC541_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2840675.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3309..3539
                     /locus_tag="PC541_003255"
     CDS             3309..3539
                     /locus_tag="PC541_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2840676.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3532..4113
                     /locus_tag="PC541_003256"
     CDS             3532..4113
                     /locus_tag="PC541_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2840677.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4692..5369)
                     /locus_tag="PC541_003257"
     CDS             complement(4692..5369)
                     /locus_tag="PC541_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2840678.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5558..6922)
                     /locus_tag="PC541_003258"
     CDS             complement(5558..6922)
                     /locus_tag="PC541_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2840679.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7050..7571
                     /locus_tag="PC541_003259"
     CDS             7050..7571
                     /locus_tag="PC541_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2840680.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3015 a          880 c         1328 g         2492 t
ORIGIN      
        1 gagtaatgaa ttatattgaa cgatatatca ataattggca gtaagttata tcaaataaga
       61 tattactatc gagtaatgaa ttatattgaa caatatatta ataattggca gtaagttata
      121 tcaaataaga tattgctatt atgtagtaaa ttatataaaa caatgtatca gaataaatag
      181 tatattaata atttgtagta tatcatgacg aataatacag tcataacaaa tagtgagtca
      241 taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata aaaacttata
      301 aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga gaagtctatt
      361 ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt aaatgaaaac
      421 atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact taaaaaagaa
      481 aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc tctagcaata
      541 aaaacaagtg atgtgactaa tctaattact tggggtcaga attttggtct tgatggtcat
      601 attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg ccaaaacctt
      661 ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt tgaaagtaat
      721 ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag tatagcaggg
      781 aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg tataaaatta
      841 cttgatacca ctataggagg tctttttaaa ggagagctaa ccacaatagc agctaaatcg
      901 ggggttggga agactgcttt ggcacttcaa ataatgttaa attcatttaa gcaaggtaaa
      961 aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag aaatatatgc
     1021 agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga aaatgattgg
     1081 aaacttattg taaatgcgat aggtgattta agcgaaaata acttaattta tatcaatgat
     1141 aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc agatttatta
     1201 atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag agaaagggag
     1261 gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat acctgtaatc
     1321 caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga tagacctatg
     1381 agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca tcaattaaag
     1441 gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc agttagagca
     1501 tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag agatggacag
     1561 actaggcata agcatttttg ttactttggg gataagttac attttcaaga actaaattat
     1621 taggtcaata aagtagtaat aacaagctta ttaaaagtcg ggaaatagca aatagattat
     1681 aaaatatgag gtgaaaatat gttggaatta agaaaaatta tagaattact tgaagaacac
     1741 aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga ataccttgaa
     1801 ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg ggctttaaaa
     1861 gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat ggaattagcg
     1921 aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttagaaaaaa atagatattt
     1981 ttggagaata caagtgtgat atacttctga aatagaagtt attataagag gagtataagt
     2041 gtgattaaca acatgatttt gtgaataaaa ttgaggatat atatgagtta aatgtgtgat
     2101 taataaggta agattgtgta taagattatg agtatttaaa aggaggggct ataattaata
     2161 gtgtagagtt gtgtgtttgc ttaagattat ataataattt aatagattaa actatactaa
     2221 taaggtaaaa tcgtgtgttt agttaaagct tattataaaa tcttgatatg tgattaatga
     2281 tgcaatatgt gaataaagtt aagattataa tagatttaac ctgtggctaa tagaataaaa
     2341 ttgtgaataa agttaagatt ataataggtt taaattgtga ctaatagagt aagagtgtaa
     2401 atagagataa acttcaatat gagcttaacc tatgattaat agagtaagaa tgtaaataga
     2461 gataaacttc aatatgagtt taacttgtgg ctaatagagt aagagtgtaa atagagataa
     2521 acttcaatat gggtttaact tgtggttaat agagtaaggt gtaactaaag ataaacttca
     2581 atatgagctt aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg
     2641 agtttaactt gtggctaata gagtaagagt gtaactaaag ataaacttca atatgggttt
     2701 aacttgtggt taatagagta agaatgtaaa tagagataaa cttcaatatg agcttaactt
     2761 gtgattaata gagtaagagt gtaaatagag ataaacttca atatgggatt aacttgtgat
     2821 taatagagta agagtgtaac taaagataaa cttcaatatg agcttaacct atgattaata
     2881 gagtaagaat gtaaatagag ataaacttca atatgggttt aacctgtggt taatagagta
     2941 gggtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     3001 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3061 taaaattata gagctgcata agaaaatatt atagatatat taaatattag ataataaata
     3121 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3181 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3241 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3301 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3361 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3421 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3481 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3541 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttgat attgggattt
     3601 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3661 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3721 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3781 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgccaa tatgcttttt
     3841 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3901 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3961 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     4021 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4081 atgatatgta aggcaaagag taagtctgta tagaaaggcc ttatgtgata tttacagttt
     4141 tgttcaagta gagatgagta gtaggtttga atatatatga caaatatatg agtatagggg
     4201 catagattta gtttgtgtaa ggtaaactga tttatgcttc ttttttatgt cttagattat
     4261 tggctaagtg gaaacaatga atttagatat taagaattat tcagttattt gaagaaaata
     4321 gaataataat taacattata ttaatggata ttcattacat ataagccagt taatagcaag
     4381 atttatttaa tatgatatag gtcaagtatt taaactaaat aaggtcatga caattaatat
     4441 aagtatagat ataaaaagat tagaagttat aacttataaa aggtattcta tataaatagt
     4501 atggggaaaa tagtcaaatt aacttattag aaacaaaaaa tatacataaa aaataaatgt
     4561 aaatatcaaa taagatacta tatcaaaata gatataagta tcttttttat tattgaaatt
     4621 atgaaaatat atttgatttt acacaaaaaa gagtgcctca tgaactaaaa agttcatttt
     4681 gagacacact tttacagtgt caaagaacga agtttatcct tatccaatac ctttatacca
     4741 ccacgagata aagacacaat accttctcta gcaaaatatt tcaacattct cgatacaact
     4801 tctctggcac tacccatata cttagcaatt tgctcatgag tcatatttat agtgctactg
     4861 ccatttttag ctatctcatc aattaaaaaa gatgccaaac gcttatcaaa actcataaaa
     4921 agaatttgtt gcatagccca cataacatca gaaaaacgtt cagtagctaa tttatatgaa
     4981 aataactctg catgaatatt ttctgtagat aattttgcaa aaacagagga actaatctgt
     5041 ataatatcgc agtccgtttc agcatctaca tagacatcaa aagttattgt ttttagaata
     5101 caggaagctg ataaaataca tacatctcca tcatcaagtc ggaacagtgt aacctctcga
     5161 ccttcatctg aaagtatgta tgtacgcact gttccagatt ttataatcaa aataccaata
     5221 cagtcactat caccacaatg tactggattc ccttttttgt aatgcgaggt atttgctgaa
     5281 gaaattaata attctttttg taagtcagta agtttatccc aaaaaggcaa gctatgtgta
     5341 agtatatcaa tttcattttg tgaaatcata caatatctcc tttcttaaat tgttataatc
     5401 ccatgataca agtgaataga taaattgaca agtagtttgt taagaggatt atttagtcag
     5461 atagtaatag tatgatttat gtagaaaaaa tatgataata tatattcttg ctattaatga
     5521 gaaagtaata tgttttgatg atagtaggtt tgtataacta atgcataccg tttattttta
     5581 aaagattaag ttccttatgt aaccttatag acataaaacc agcaattata gcagccatca
     5641 aatcagctat tggttgtgca tataagatac catttaaccc atagaataca ggaataatca
     5701 aaattatagg gataaaacag acaccttgtc tacacatacc taaaaatcca ccttcctttg
     5761 cttttcctag tgcgaggaat aatgtagaat acactgtata gaatccaaat agcataaatg
     5821 aaaacccatt tgccctaaga gctttactac cattttctat taataaaatg tcaccacttg
     5881 taaattgtga aattatagat tttgggaaga gtatcaatat aagaccaagc actacacaaa
     5941 atactgtagt ccataatatg gaagttttta ttgctatatg taatctatcg tagttctttg
     6001 aaccaaaatt ataaccagca attggttgga aaccttttat aaaaccaaat acaacaagac
     6061 tacctaaaga cgttattcgt gttacagcac ccattccagc tatcacagaa tcaccatact
     6121 ttgtagcctc catattagtt aatgtaatag caagactagt caataattga aatattaagg
     6181 taggtatgcc aatttttagg atttctgaaa aaatttcttt tgaaaaacag cattcgttaa
     6241 ttctaaaact aaatatactt ttcttgctta aaatatatcc tacataaaca agtgttgaag
     6301 caatctgtga gatagctgtt gcaatagaag caccccttac ccccagacca aaagtataaa
     6361 tgaatatcgg gtctaaaatc atattaagca aagcacctaa taacatagca cacatagttg
     6421 ttttagcagc tccttcactt gtgacaatat tagtcatagt gacattgaat atattaaaaa
     6481 tagatgacac gacataaatg ctagcataag atattgcata aggcataata ctttttgtag
     6541 ctcctaacaa ttttaaaatt ggattgagaa aaatcataat cacaataata actaatgtac
     6601 ccactataat actactgtaa atagatgtac ttaccacttt tcctgctttt tcattatcac
     6661 catggcctaa aagtctggca acatatgaag cagctccatt tccaaacaat aatcctaatc
     6721 ccacaacaag ctgacctaat ggaaatgcaa cggatatagc tcccatttga cttgttccaa
     6781 gtccacccac aaagtatgca tcaaccaaat tgtataatgc agttatcatc atacctatca
     6841 tggtaggtaa tcccatagat aacaatgctt ttgagattgg ggcagaccct aataattcaa
     6901 ttcttttgtt actttctttc atacttaaca ctcctcttct attgacatta agtctaaatt
     6961 ataatactat aaattatagt aagtatggaa aatagtacta taatttatag tatttgtcaa
     7021 tagtaagaaa actaaaggag atgattttta tggctacaat agatttaatt gtattgggaa
     7081 tagtaaagca acaacccaag agtgcttatg atattcaaaa ggcagtggaa tacaggaata
     7141 tatcacgttg ggttaaaata agtacaccat caatttataa aaaagtaatt caattagaac
     7201 aaaagggata tattgaaagt aaaactgtga agaatggaaa aatgccagaa aaagcagttt
     7261 attcattaac tgaattaggt gagacacatt ttttagattt aatgtttgat acatctagca
     7321 aaccaataaa tattctttta gattttaatg ctgttatagt aaatttaaat agccttacac
     7381 cagaaaaaca gaaattatgt ctcaataata ttgaagagaa tgttcaagag ttaaagaaaa
     7441 ctattgagga aaatataagt ataaagacag aagtaccaga cacagcaaaa gcagtgttgg
     7501 agcaacagtt ggtgctagca caagcattgg aaacctggat aaattcttta aaagataata
     7561 tgggaaatta gaattagaga agctaactta ataatcatta aacaaaaacc agcatattta
     7621 ataatgagat attagaaaaa ataaaacaaa tgatataaac tgaaaagtat atttaaaata
     7681 aagctcactt aattgaggga gaatgttaaa ctttt
//
LOCUS       DAMJZE010000180         7661 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJZE010000180 DAMJZE010000000
VERSION     DAMJZE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.184"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..1311)
                     /locus_tag="PC541_003260"
     CDS             complement(<1..1311)
                     /locus_tag="PC541_003260"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2840681.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKE"
     gene            complement(1460..3265)
                     /gene="lepA"
                     /locus_tag="PC541_003261"
     CDS             complement(1460..3265)
                     /gene="lepA"
                     /locus_tag="PC541_003261"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2840682.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3547..3798)
                     /locus_tag="PC541_003262"
     CDS             complement(3547..3798)
                     /locus_tag="PC541_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840683.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3829..4848)
                     /locus_tag="PC541_003263"
     CDS             complement(3829..4848)
                     /locus_tag="PC541_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2840684.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(4866..5819)
                     /locus_tag="PC541_003264"
     CDS             complement(4866..5819)
                     /locus_tag="PC541_003264"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2840685.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6161..6937)
                     /locus_tag="PC541_003265"
     CDS             complement(6161..6937)
                     /locus_tag="PC541_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2840686.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6971..7492)
                     /locus_tag="PC541_003266"
     CDS             complement(6971..7492)
                     /locus_tag="PC541_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840687.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
BASE COUNT         2359 a         1376 c          861 g         3065 t
ORIGIN      
        1 ttcttttagt atttcttttt catttaactc tggattattt atgactattt caagcatctt
       61 atttaaaata tatcctatat ctttacctgg ttttatacct atagatatca aatcttttcc
      121 attaatagct aaatctttaa gattaaagca ctccttttga tttattatat gtttaaattc
      181 tttttcacac tcaataagat ttaatatctt ttgttttgca tactttggat tttttgcaag
      241 tgtatcagcc caattaactt ttattaaatc ttcaaataat tcaacatctt caagtttatt
      301 taataacttt ttaattatct tccttttagg ttctattcta taatcatgat attgtattaa
      361 tattaaaact ttattaatag tataattatc atacttaagt cttttcaaaa tcttctttgc
      421 aatctttaca gattctctac tatgagtata aaaatggtca acaccatttt tatcagttgt
      481 tttagttact tttttaccta aatcatgaaa caacattgta agttttaaat acagttcatc
      541 ttcaattact tctgtagcta ttaaagtatg tttttgtaca tcatagatgt gatatggatt
      601 gtgttgagta acatcttcta attcacatat ttcaggaatt ataaatttca acaaacctgt
      661 tgattttaat aagtttaatt tagatgaatc agatactaat attttattaa actctgtctg
      721 gattctttca atagaaacat tttttatcaa atctgctttt ttatgaatac tttgcttagt
      781 accttcagct attttaaaat tgagttgtgc tgaaaatcta actgctctaa gcattctaag
      841 tgcatcttct tcaaatctat ctactggatt tccaacacaa cgtatatact tattttgtat
      901 atcttcatag ccattaaatg ggtcgactaa tcctgtcttt gaattgtatg caattgaatt
      961 gatagtaaaa tctcttcttg acaagtcctt aactatgtta tttgtaaatt ctattgaatc
     1021 tggatgtcta ccatcactat aatttccatc aattctataa gtcgtcactt caaatggttc
     1081 atgttcaatc ataactgtta cagtcccatg ttgtatacca gttggaattg ttttttcaaa
     1141 taattctact accacttctg gtctagcact agttgttata tcccaatcgt ttggaactct
     1201 ttctaataaa caatctctaa cacatcctcc aactatataa gcttcataac cattcttttc
     1261 taattcttta attatatagt ctactttttt aggaatttct atattaatca taaatatcac
     1321 ctttttattc ttctttaata ttcttactta tattctctat gagtttactt aaaatatacc
     1381 ataaaaaagc tagactaatt tagaaataat tctaaaatca ctctagcttt taaatatctt
     1441 tatctaaatt tataaggatt tactcatcta atttaagcac agacataaat gctttttgag
     1501 gaacttcaac agaaccaatt tgtctcatac gcttcttacc ttctttttgt ttttcaagaa
     1561 gtttcttctt acgagatata tctccaccat aacatttagc aagaacatct tttcttagag
     1621 cgcttattgt ttctcttgca ataaccttat taccaacagc agcttgtatt ggtatagcaa
     1681 actgatgtct tggaatttct ccttttaatt tctcacacat tgcctttcct ctagggaaag
     1741 cacgtgtctc atggacaata aaactaagtg catccacttg ctctttattg attaatatat
     1801 caagttttac aagtgtagat gctacataac ctttaacctc ataatcaaga gaaccataac
     1861 ctctagttct tgattttaaa gcatcaaaga aatcgtaaac aacttcattg agaggaaggt
     1921 catagtgaag cattactctt ctatcatcta tatattccat atttaacata tttccacgtc
     1981 tttcctggca aagctccata acaacaccca catagtcttt aggaactatt atatctcctt
     2041 ttactatagg ctcttctatc atctttatct cagatggttc tggtaagttt gcaggatttt
     2101 gtatcattac aacctctcca tccatttttg taactctata tataacagat ggagctgtag
     2161 taattatatc aaggttaaat tctctctcaa gtctttcttg cattatctcc atatgtaaaa
     2221 gacctaagaa accacatcta aaaccaaatc ctagtgctgc tgaagtctca gcttcatatt
     2281 caagtgcagc atcatttact tgaagctttt caagagcatc tctaacattc tcatactttt
     2341 ctccttctcc tggatatata ccacaatata ccattggagt agcttttttg tatcctggta
     2401 aaggttcttc tgtaggatta tttgaatctg ttattgtatc accaacacga caacttctta
     2461 tatcttttat actagcagca atataaccaa catcacctgc tgataattca ctaagttcag
     2521 tttgacctgg agccataaca ccaacttcag ttacctcaaa ctttttattg gtattcatca
     2581 tttttattgt catacccttt ttaacagttc cttcaaatac tctaacataa gctacaacac
     2641 ctttgtaagc atcatagtaa gaatcgaata ttaatgcctt taatggagct tcattatctc
     2701 ctttaggtgg aggaacattt tttacaatat cttctaaaac atcttctatg tttaaaccag
     2761 ttttagctga aataagagga gcttcacttg aatcaagccc tattaaatct tctatttcat
     2821 tttttacttc ctctggtctt gcacttggta aatctatttt atttattatt ggtaatattt
     2881 ctaaatcttg gtctatagca agataaacat tggctaaagt ttgtgcctca acaccttgag
     2941 ctgcatcaac tactaatagt gctccttcac atgcagctaa acttcttgat acctcatagt
     3001 taaagtctac atgtccaggt gtatctataa gatttaaata atattcattt ccatccttcg
     3061 ctttatacat taatcttatg ttttgaagct ttatagtgat tcctctttct ctttcaaggt
     3121 ccatattgtc aagtaattga cttttcatgt ctctttcact aacaagccca gtttgttgta
     3181 ttaatctatc agctaaggta gactttccat ggtctatatg tgcaattata ctaaaatttc
     3241 tagttctact ttgtttattg tccaccttaa aattccctcc tcttgattat tcttatagtt
     3301 attattttac attatctaaa aactctttat ataccaaatt taaaattttt gaatataaac
     3361 taattggcac taaacaaaat tatccacatt atatgtggat tatcaagtct aacactaaat
     3421 attatataat atcataaact taaagtaaat cctataaaat acagttaatg aatttttatt
     3481 ttatacatta aaaatatata actaaaataa ggcttatttt aatcaataaa ccttattcac
     3541 ttattactac ttattaaatt tttttatgat ttcatgtata tcaaaatttg gtatgtcaaa
     3601 atcaaatttt gttatatcaa atttaatact tggaaaatca tattgtttca ttcctagcat
     3661 actttttgca ttactgtcca ctataaatac acaaactgat gtacatataa tcatagtaat
     3721 caaaaataag attctcataa tatacttgta ttttcttagt ttacttttac tttctatatt
     3781 tttttctaat cggctcattt agcatgctcc taattataac ttaaagctct aatttttttg
     3841 tttaaaatat tcatccaaaa tctcggctaa atactttgta gtagccttag attcttctac
     3901 acttgttgcg ttattcccta tttctactaa catatgattc ttaactgcaa actgattgaa
     3961 tctagcatag tctttttcta ctattggaga tactagacca ggatactttt tctcagcaaa
     4021 agcagttata tcttctgcta aagctctaag ttgttttcta tttgtatttt tacctcctac
     4081 aacaaagaag aactttgata ctctctctcc atttatcata gttgtatatt ttaaatggtc
     4141 tttttcctta gtggctggat tagttaaatc cctagcatct ctatgtaagt ctatcgctat
     4201 atccactgag ttatatttag gtaatatact ttgtattgtt tttaagctac ttgcataaga
     4261 gttattataa gatggatagt catggtattt agtagtatgg actacacccc atcctttgct
     4321 atctaaagca ctagttaatg cacttcctac ttccattact gaatttttct tatctcttga
     4381 atgataattt ccatcaggtg aatttgaata agtctcacat ccatgagtat gatatatcaa
     4441 aattcttgga ttttctttgg ttactcttaa atcatcctta taatctgaac tagatgttgt
     4501 gttagtttca gttgtgtctg aattttttga ctcaatatct ggaacatttt ctttaccaac
     4561 ataaagtttt atatattctt tttcactctc ttttttatta tcaactttac ttgcatcttt
     4621 agatttagtg tttccttctt tactttcttc cttattttcc ttacttgttt ctttattttt
     4681 attattcttt ttattttctg tatcatttcc ttcgacttta gcttctggat aagatgaatt
     4741 tactaaaaac tttaaaaaat catcttgatt caaagctaat gattttcctg gtaaaatgca
     4801 tactaatata aaagtcaagg ttactacttt aatgcatttt ttaaacataa ttgctcctcc
     4861 ttattttata ccaatctacc tggatgtaaa gacctattta agccttcact tattattata
     4921 gatagatttt cgattgtatc atctatatcc tttggtgtta ctattaagtt cttgtcatat
     4981 gggtctaacg aatcttttat tagatgatat ttttcttctt ctttaagttt tttcaacatt
     5041 tcataaaaac tttctgtttc ttctgattga gctattaaat tgtctattgc catatctaag
     5101 acatctattg taagagttgc tgcatcaacc accgttggaa cgcctattgc aataacatcc
     5161 acgcctaaag tttctttagt tagagatttt ctagtattgc caacacctcc accaggtgat
     5221 attcctgctg ttgatatctg tatagtagag tttactcttt ccatttttct tgaagccaat
     5281 gcatctattg ctactactct atctggctta accttctcaa ccaatgattt tactatttca
     5341 ctagtttcta ttccagtaac acccataact cctgggctta aagctgatac ctcagtgaaa
     5401 tcatcatcat agtctttatt ataattttta aaaagatgtc tagttacaag tgtttttgat
     5461 acactctttg gtcctaacgc atcagaagta atatttctat ttccaagacc aattaccagg
     5521 gtttttttat ttttgtcttg gccaaatata tctactaact cctcttttaa ataacttatc
     5581 atttcttctc ttgactcatc atcatcaaac ttcataagat tactttccag agttatatac
     5641 tttcctatat ctttattcat tatctctgcg ccttgcctgt ctattacttc aactttagtt
     5701 acaatgcaat tttctaattc tttagtttct atttttacac caggaatact acttccttct
     5761 tgagactttt cacacatttc actagcttct aaagctaaat ctgttcttac actaatcata
     5821 taaacctcca gtataaatta aatgctaatt ataattgtta acaaaattta gtattttata
     5881 acaagaaatt gtaaatttat ttaaaacaag taaaattaag gctttcaatg taagaaaatt
     5941 gggtttagtc agtgatttgt cagtaaacct tcttatctat catttattta caaacagaaa
     6001 aaggtagcaa ctatttatat aggtgctacc tttaatctat ttataatttt cctattaagc
     6061 tttcactaat atattaacta aaataatttt ttatatttat acctaattta tttactatat
     6121 aaaaaaacag caataattat acaataatca cctaacacct ttattccttt tggaaaaatg
     6181 gttctgcaat ttccaaccat tcatttggaa gataaagtga taaataccca tgattatagc
     6241 tcttagcttc ataaatctca caagagggca cataagactg aaacagtttt gcagattttt
     6301 tgacacactt catttctttt tctccatacc agtacattac ttgtgctgtg gtttttttaa
     6361 cattctcttt aagtgtatag ttcatcatgt aagtacgata catattatag aatgtttctt
     6421 ttcttacatg tggcatatct gtcatataat acgcttttat tttatctgga tattgcatat
     6481 tttcaggtaa caattttggc atcatagcaa tttgaaagcg acaagccttc tcactaaaca
     6541 aaagtcctcc aaaaaatcga ataccagcaa tacaaaacct tgccataaga ggtcttgggt
     6601 agcaaatact accatcaata attgcttttt tagtaatatt aggttttcga gataaaagtt
     6661 ccatgacaat ttgaccacca agtgataccc cacacaaagc gaaaagatgt ccactgcaat
     6721 tttcatctat atatgatatc aacttatctg ctgtatcctc tgtagaaatg tatgttgttt
     6781 catattcttc tccatgacca tccaatgtag gaagtatcac atgatatttg ttagacaaaa
     6841 cttctgcttg tcgcaaatag ttccaccatg cattccctcc cccatgaatc aacataatat
     6901 gtggtctgtt tttatttcca aactcatgaa atttcatata taacctcctt ttataaaaat
     6961 gggttgcatg ttattttgtt ttatcattat cagctacagg tggagaagat ataattgaaa
     7021 gttttctaag ttttagattt gatttaggtt tttgttctat atatggtttc aataaatcag
     7081 ctatactttt aagaagattt tcatattcat tatcactcat atatagtaat gctgaagaga
     7141 aaaatacatt atccttttta tggtcttgct cttttctgct aaagtattca ttcatttcct
     7201 gcatcagacc tacataaaat gctgtagaaa gtgcaacgaa ggtctctcct tccatagatg
     7261 ttgtatattc ctttagtgca taaacctttt caatagtacc acgcacacga ttttctttaa
     7321 ttacttcaat catattgttc tcttctaaaa tttttacatg gtgataaact gttgcacgag
     7381 gaatatcatt taaatacttt acaatatcag atgtacgaac actgccataa agtcgaatat
     7441 attgtaaaat acgaagcctt gcaggattta aaattagttt tgctatgtcc atcttatcag
     7501 ccctttcttt agtctataaa aatattatag tctatttttt tagactattc aagtctatga
     7561 tataaatagt aattacaact ataataaatg ctacaaatgc tattctatat aaacactgac
     7621 attaattaca tataaccttc taacatatta gttatattgt t
//
LOCUS       DAMJZE010000181         7630 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJZE010000181 DAMJZE010000000
VERSION     DAMJZE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.185"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            115..1389
                     /locus_tag="PC541_003267"
     CDS             115..1389
                     /locus_tag="PC541_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2840688.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(1550..4591)
                     /locus_tag="PC541_003268"
     CDS             complement(1550..4591)
                     /locus_tag="PC541_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2840689.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            4797..5096
                     /locus_tag="PC541_003269"
     CDS             4797..5096
                     /locus_tag="PC541_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2840690.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(5335..7563)
                     /locus_tag="PC541_003270"
     CDS             complement(5335..7563)
                     /locus_tag="PC541_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2840691.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         2639 a         1095 c         1078 g         2818 t
ORIGIN      
        1 taataaatta ctagaggtat ttaactattt agaaagaatt ggtattatat aaagtaagag
       61 aatatataat aagaaagaat atttgatttt attggaaaac aaaagaaaaa gaaggtgatt
      121 actatttctc atataactgc gaagaatatg tataaaagtt tagaggaaag aataaacaag
      181 tttccacaag gtgctccacc atctgataca ttatacaaaa tactaaatgt actatataca
      241 gaacaagagg cgaagttagt tgctcagctt ccaataaagc ctttcagagt aaaaacagca
      301 gctaaaattt ggagtgtaag tgaaagtgaa gcatatagag tattggataa acttgctagt
      361 aaggctctga tactagatat agaagataat aaagggaaga aatatataat gcctcctcca
      421 atggctggtt tttttgagtt tgccatgatg agaacgagac acgatataga ccaaaaactt
      481 ttggcagagc tttactatca atatatgaat gtagaagaag attttatcaa agatttattt
      541 tattcaacag aaacgaagct tggaagagta tacgttcaag aggaagtttt aactaatgat
      601 aatgaggtta gtattttaga ttatgaaagg gctactcata taattgatga atcaactcat
      661 ataggtataa gtatgtgtta ctgtagacat agaatgcaac atgttggaaa agcttgtgat
      721 gctcctatgg atatatgtat gacgtttgac aatgtagcaa attctttaat aaataataaa
      781 tttgcaagac gtgtggataa aatagaatgt aaagagctac ttcatcaagc atatgaacat
      841 aacttagttc aatgtggaga aaatgttagg aaaggggtta cgtttatatg taattgctgt
      901 ggatgttgct gtgaagcaat ggtagcagct aaaagatttg gaaatcttca tccagtacag
      961 acaacaagtt ttataccaaa tataaaccat gaaaattgtg taaagtgtgg taaatgtata
     1021 actgcatgtc caatagatgc gataagcaag gttaaggaag atggtaaaga atatataaaa
     1081 attgatgagg atagatgttt aggttgtgga gtttgtgtta gaaattgcca taaaaatagc
     1141 attatgttat taaagagaga tgaaaaaata ataactccag caaattcagt acatagagca
     1201 gttcttatgg caattgaaaa agggcagtta caaaatttaa tttttgataa taatgctctg
     1261 gctagtcata gagctatggg agctatactg tcagctatat taaagcttga accagctaaa
     1321 aagattttag ccagtaaaca attaaagtca gtatatttag ataaattgtt aagtatgaat
     1381 gataaataga aactaactta aaaaaggcaa agttttagac ttatctatat cttaataaac
     1441 ttatcattta atatttaaat ttaattttta cacatataaa acttataact taaataaaaa
     1501 aaggctatat tcaattaata ttacttgaat acagcctttt ttagttatgt taagctattt
     1561 cttttatttg cttaaatgag ttttcatttc catttccacc aatttgtgtt acagatttaa
     1621 atctcatagt tttaaatagt tctttttgat tataatctag tgagtttcct actagcatta
     1681 caggagattt agtttttcca gccaaaactc caacagataa tccgtctatt aaatcatctt
     1741 gtttattcat tccattttta gcaacatata aattgtctat cttagaatcc ttgtaaaatt
     1801 cttttattac ttttgcatta gtttcatttc tagtgctacc tgatattctc tgtggatttt
     1861 gaagtttact ttctatattt ttagaaactg tatattctcc accgattaca taagatttat
     1921 caatcttttt gtttttaaac aagttattta tttcagttat attactattt tcattagtaa
     1981 gtattatagg catatcattt tgagctgata cagctccaat gcttactgca tcggctaatc
     2041 ctttttcgcc attaactact gaaatctttg atatattgct aatattgttc aattctttag
     2101 caatgttcat agaagtttga tatctatcac ttcctgaaat tctagataca gagatattgt
     2161 ttgattttat agtatctaaa gatttttcat ttatagaagc ttctccacca actacatata
     2221 catttttaac ttttaatctc tttaattcag ccaaagtttt ttcatttatt tgagagcttc
     2281 cagtcaataa tataggagcg tttttcttgt atgcaaatgg tgttgcagcc aaagcatctg
     2341 caattgctga atcgtttatt aaaactgcat tttcagcatt ttcccaacct gcttgactta
     2401 tcttaataga agtttcatat ctatcagttc ctgtaagtgc acttgcattt acttcatctt
     2461 gatatgtaac ctcaaatcta gcatttggac ttcctaagaa aacaattacg cctccttttg
     2521 gcaatctaac tctattgttc tttgtaacta aagaatagtt tacaggaaca cctttatcat
     2581 cataaacagc aaaagctgaa ttttgaggaa gattaacttc tattttttta tttgcgatat
     2641 cgtcaccaat cttataccat tttgcataac cattggattc tagttcacaa gtaaaagatt
     2701 tttctgctgg caaattagtt atagaatctt cactaacata cgttagtgtt ccaaaagata
     2761 aatactctgt accattttct ttatgaagtt taatgtcgga taaatcacga cctattacac
     2821 caggtatttc tataaaagca tttgagttat tctcatccat tattttagtg ttaactatat
     2881 aacctggagt ttcatcagaa agagcaagag ttgccttcac agaaccaaac atatagcttt
     2941 gagaagtata tttttcatct actagatagt aatctttacc atttcttttt ttccatgctt
     3001 cttttacact atttgatata ttgttgtcat ctatcttttg agctacatag tataaacttg
     3061 ctgtctgacc taatccaggg acatcatcat aagagctcat atttaaatat gtgatattgt
     3121 ttttttcttt tacaaatttt agacagctgt tacctttttc agatacaaat ctatcttgac
     3181 ctatgtatac gtatttatct tcaggtccat tttctatata aggagaagat actgttaaag
     3241 taccattatc attaacatct acttttatca tatttgaaga agcataaaga ccactatttt
     3301 cttttagaga gctaggcatt ttaacttgtt gtggtttact aaatgtttta tcaggtttta
     3361 tttctttaat tttacctttt tcttttaatg cagatagtaa gatttcttgt ccaattattt
     3421 cattaagttg actacttcca ccagaagaaa gaactgcaac tgccatattt tcatctggaa
     3481 gaactataag attactgtgg aataataaag agtctccacc ttttgtaaga gcttttaaat
     3541 tatactgatt aaatggataa gtgttgacac aatcccaccc taaaccatat ccaagtatag
     3601 agtcttctcc ttcaggccat agaccattta aatattcttt gttttccata gcttttactg
     3661 aagcaggaga aagaatacca tttgaatttt tcataaatgt ttgtgcaaat gtacatagat
     3721 tttctgcact tgaatataaa ccacctgcac ctatagcatt aaggttatct tgcggaactg
     3781 catcttccca ataaggcacg tatgcttttg ctagttttga actatcaaaa ctattttctg
     3841 tagtttttgt attttgtaag ttaagtggat tatttatgta tttatcaaga aaattagtaa
     3901 atgacatacc agatactctt tctactaaaa tttcagctaa agtaaatcca tcattacaat
     3961 aaacggaaaa agctcctggt ttagctttta atctttgttt ttgtaattct tttaagaaat
     4021 tatcatgacc ataagaatca ttgtctgcta ataaaatagt gtttttaaaa ctacttccca
     4081 tcaaccctga tgaatgattt aataacattc ttggagtgat ttccttgtat ctatcatcag
     4141 ccattttaaa ttcaggtata tactttacaa caggagtatc taaatttaat tttccatcat
     4201 caaccaactt cataacagct gttgtagtaa acattttact gatagatgcg atactataca
     4261 tattatcctt atttaagttt ttgttatcct gtttgctgta aactccacca ttaccagaaa
     4321 tctcaatttt gccattatca atcaaagcat attgagcact tacagcacca tactttgata
     4381 gcaaagtttg gattttctct ttagcaagag ctttagtttt tgtgtaagat tccgcgtttt
     4441 tactataatc atgtaattct atatcagaac tttgatattt ttcggaatta tcttttgtat
     4501 tatcagcaaa agtaatattt ggagcagcag taaataacat actcaatact aatgtactag
     4561 ataataattt agagagcctt tgcttaaaca tattacatta cctctttccc tttttaataa
     4621 tttataatta gcataacatt cttaattatt ttaatcaaca gattattatc aataaaaaaa
     4681 gcttttataa gtatagcttt ttatataaca gagaaaaata taaaaataat atttgttgtt
     4741 gtataaagaa taaagggtat aggtaatata taaataaaat ggaggaattt tagacaatgt
     4801 ataagaatat aaataaaaag caacttaaag aaatgatgaa aaatgaaaag gatgttttat
     4861 tattagatgt aagaactaaa gatgaattta aggaatataa aatagaaaat gctataaata
     4921 tatctttaca agagttaatc aataatatag atgaaattta cgattataaa gataagaaag
     4981 tagtagttta ttgcagaagt ggtcatagaa gtgttactgc atgtaatttg ttagctgaag
     5041 aaggttttga gcatttatat aatttaaatt caggaataat tgattatatg agttaaataa
     5101 tgtaagttat acattaaata ctatgtatag ctctgcttat taagtaggaa tatatctaaa
     5161 tatttataag ataaatttag gaaagaacga attttttcgt ttttttctat gacagttttt
     5221 gtagtaaata ttagttttta agttggaaaa aggtagtagc tttttagcta ctacctttta
     5281 atatgaactt agaatattat taaaaaaaat atatttaaag acagaattaa ttttttaatg
     5341 accagttaaa ttactatcgc cgaatttatc attaaatggt tttataactc tatttatagg
     5401 tccttttaga agtacattta aacaaataga cagaatcata aatagtaata gaactagtat
     5461 atctttagtt agatttggag catatatacc accaactgct tcccttgtag cacctattgc
     5521 ataggtaaat ggcattatag ggtttattct ttggaagaat gtaggtgtaa cctgtatagg
     5581 gaatgtacca ccagaagaac caatctgaac gactaatagt actattgcaa ttgcttttcc
     5641 tacatttcca aatacagaaa ctaaagaata cactataaag ttaaatacca tactagtaaa
     5701 cataagcaga actatcaata aaattggatg ttttgctgtt actcctaaca ataatatatc
     5761 tccagttcct attataaaac cttggacaag agctatactt aaaaaagtaa ggcctcttcc
     5821 aaagtaaatt tcataaggtt tatagtttcc atgtacagat gtagacaata gtgctgaaat
     5881 aagtaatata ccaacccatg tagaaagaac actataaaat ggtgtcattg cagaaccata
     5941 attttcaatt ggatataatt tctctgtttt cacctcaaca ggttctttta agaaattaga
     6001 ctttgtcaat atatcacttt caagcaagct gactaatttt ttcatatctt caccattact
     6061 aattttgctt aaagtatcaa tcaaatcatc caacattcct tttgctaaag gaagtctttc
     6121 tcttatcaat gatatacttt cctgtgcatt tcctgaaagt tttagagaag tagtaagtat
     6181 ttcttcaacc tttggaagtt ttgcttctgc cttatctaaa acagtaatta tatcatttgc
     6241 tacttttata ctgtttgcaa atatactatt tataggtttt gatatttttg agtcaaaatt
     6301 atttaaaata tttaaattaa ttcttccaat accatttgat agacttaaaa cattattcaa
     6361 agcacttata gatggttgtt gtccagatat aactttgttt ttaatatcat ttaatgttga
     6421 tatagatgaa tctattttat tgctagagtc ttcaagatta tctattacat catccaatct
     6481 attatttgat gtaagttggt ttaatttagt taaaaagtcc accaaagtat cgtttaaaga
     6541 ttgcaaatta gaaagttttt ctgataaatt atctatcaac ttaggtacat cttcactgcc
     6601 actatttact gcatcaatta aatttaatgt aagagaagaa gcagaagatg aaaggtcaac
     6661 cattaaattt aagtcagact taataagagg agaaagttcg tctaaattat catttgtatc
     6721 ctctaagaat ttttttaagt cacttgataa tagttttgta tcattcagag tctttttaat
     6781 tgtaggtaaa tcagatttaa tatctttaac tgtatcagat aattttgaag tagcatcaga
     6841 agctaaatca acagtcttat ttatatggtc aaatctcttt tgaaggtcaa taagggaatt
     6901 ttcaactttt gtcaattttg gaagactgtg ttcaattcct gcaccaactc cgtttgatat
     6961 accaagaacg atttcactaa ctgtttttac tatagtctga ttaacctgta gttgtattgt
     7021 agaagcacct ttatctgtaa tctttggtgc aactgcattt attttttcat taacagtata
     7081 gataatttcg ccttttttaa catcatctgt aattaaagat gtcaaatctt ttgtaaaatt
     7141 ttttggaatc ttaagtgatg cataataagt acctttttta acaccttcaa gagcagtttt
     7201 ttcatctaca aactgccaac ctaaactctt gttatctttg agttgttcaa ttaattggtc
     7261 tcctacatta atcttttgtc ctgctatttc atttccttta tcttcatttg ttactgctac
     7321 aagcatatta gcagtgcttc cataagggtc ccaagatgcc tttatattga accaagcata
     7381 cagagatggt aaaaaagcaa gacctaaaac tattacaagt acggcataat tgccacgtat
     7441 atcttttagg tcacttttaa aaatttctaa tatatttttt aaaccctttg aaggtcttaa
     7501 attcttgata ttattccagt ttaaaatttc ttttaaacta ttagtttttt tcaaatgtct
     7561 cacctcctaa aatattttga taaagaaaaa agttagattt ataatcaatt ttaaattata
     7621 ttacttttta
//
LOCUS       DAMJZE010000182         7499 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJZE010000182 DAMJZE010000000
VERSION     DAMJZE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.186"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(69..281)
                     /locus_tag="PC541_003271"
     CDS             complement(69..281)
                     /locus_tag="PC541_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840692.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(282..698)
                     /locus_tag="PC541_003272"
     CDS             complement(282..698)
                     /locus_tag="PC541_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840693.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(766..1179)
                     /locus_tag="PC541_003273"
     CDS             complement(766..1179)
                     /locus_tag="PC541_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840694.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(2012..2641)
                     /locus_tag="PC541_003274"
     CDS             complement(2012..2641)
                     /locus_tag="PC541_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2840695.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2720..3682)
                     /locus_tag="PC541_003275"
     CDS             complement(2720..3682)
                     /locus_tag="PC541_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2840696.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(4444..>7499)
                     /locus_tag="PC541_003276"
     CDS             complement(4444..>7499)
                     /locus_tag="PC541_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840697.1"
                     /translation="AEGDANAVVVEEEATAEEPTSVGDFKISGGEINVDFKFASGVLT
                     ILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTLTPDKDGGAY
                     AHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVPEGGKIPRDV
                     TLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIIINGGAITAIG
                     QESSRNTGYGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGS
                     AFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFK
                     SLGGAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTD
                     AQGILYLWFSDQFKGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTI
                     DDTPPYLIIKRYDGLPFEEDVEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRL
                     NEETMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPA
                     DTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKD
                     PVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEK
                     KLKLDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTE
                     GGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTG
                     NPNLNKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPD
                     PDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGD
                     GKPDINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTD
                     ESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         2444 a         1332 c          794 g         2929 t
ORIGIN      
        1 tttaacaaac ttaataatta aataactata ttttattatc catcttataa atgtttataa
       61 actttccact attcttcttc aataaattca aacaaatttt ctactgtagt atcgaatact
      121 ttagcgatat ccatagctaa cttcaaagaa ggattatatt tcccattttc taagtgtaca
      181 atagtctcac gtcttacccc aaccaattta gcaagttcat tttgcgtcat aaggagtttt
      241 tctctgtatt ctttaatttt tgttttcatc aatattgcca cttagttaca tatccccctt
      301 ttatcaaagt atatatacaa cactaattta gataaagact gtataaataa aactgcaatg
      361 aggattatac ctgtatctag gtttgatata tttatattac ttgtataagg tgttgcaaag
      421 aaaatagata ttattactat agaatatatc attattttta ttgcaatctt attaaccttt
      481 gatagtataa attcagatga ttcatcaact aaatcattat attttttatt ataaattgaa
      541 taacatccaa aaactattaa ccaactcaac cctggaagag aagcagtgct tggaccttta
      601 aaaatatact tagtggctaa tacagctata aaaaaataag ttattacaag caactccagg
      661 ataagctttg atcttacacc agtttctgat ttagacatat aaaatcactc cttaaaaatt
      721 atattctcta tactctttaa ttttcgtttt tatcaatatt atcacttagt tgcatattcc
      781 ctttctatca aaatgtatat acaaaattaa tttagataaa gattgtataa ataaaatagc
      841 taagagaatt acacctatat ccaaatttga tatatccaaa cctcttgtat aatttgctga
      901 aaagaaaaca gctattagca aaatagaata taacattatt tttgatgcaa tatgatcaac
      961 ctttaataac ataagttttg acgcctcgtc aactaaatca ttatattttt tattatacat
     1021 gaagtcacat gcccaaaaag cgaataaaat tataaaagta atacaaagat ttccctcttt
     1081 aaaaataaac ttttttaata atgagtctat aaaaaaatag gatatcataa gtaactctga
     1141 aacaagattt gttttaacac caagctctga tttagacata taaaatcacc ccttaaaagt
     1201 tatatattta taacacctta tgttataaat atataacttt tccaaataaa ttgcaataga
     1261 atattgaaaa tattcaataa tatcttcata aatatatttt ataaaagatt ttataataaa
     1321 cattacaata cagtactaaa ttagtttatt tcatctatca tctcaatcat atcatttact
     1381 aactattatt ctaatgagca tataacattg aaacttataa ttatacaacc taaatgcctc
     1441 ccaatgtacc ctcaaaacat catttcctgt aactgaagta taattggtat attttatcac
     1501 tgaaataaaa aaacactagt caataaagac tagtattttc aagtatttta tcattctttt
     1561 tttaattatt ggagacgaat catcagaata atgcccataa tttatctata tgcaaattct
     1621 aaatttaaaa taaagtttca gataaattta ctattctatg ctatgataca acagaaaaaa
     1681 ctgaaatcta tgagtggtag cttcatataa tcctagttcc tacagtaatc aaaactgagg
     1741 tttattctat atgtaactaa tgataaattt tattatagga gttattagat ctatttgtgt
     1801 tactataagt agtaattata tatatgataa ttttttaaca cttttctgtt ggctatatag
     1861 aaagtggttt agaatttaaa tttaaataaa atttaaattc accaaaaaga aatctatatt
     1921 agaaaccact actctattcc accagattgt aattttttgt tctttatatc acaatgctat
     1981 accatattaa attgaaaaat aagttttaaa tttatttgtt acaattcatt aattcaaaaa
     2041 aatattttga gtcatgagtt ttgtaaagat ttagccatga atttaatgct tctgattcaa
     2101 aaacatctaa aagtttcaat acttgaaacg taaattccaa cggagcaatt cctgaagccg
     2161 taactaaatt ttcatcacta actacagatt ccgttttata atatttttct ccagagtaat
     2221 ttggtattac cattttaaga aactctaagt cattacttgt atgatttcta aaatctaata
     2281 gccctttttt agctaggcct aatgtagctc cacatatggc tgcaactata gaatcttgtc
     2341 ttaaagcttc tttaactttt tctaatatta tctcatgatt catagataac caagtatttc
     2401 cccctggcaa aattaataaa tccttacttt ccatattaaa ctcatcaact gtaatttcag
     2461 gtaaaacttt taacccaccc attgttataa taggattttt atcaattcct acagtaatta
     2521 cttttagtgg ctttaatcct tttttaaaat atcttcctgt atttaattca gcagttaaat
     2581 atgccacttc ccaatctgac atagtatcaa atacatacat atatactttt ctatttttca
     2641 tcttttttac tccttatgta attaatttat atatctaatt ataaaataac ttaactgaca
     2701 tattcagtca gttaagctat taaatttgat aataatcaat taattctagt aaatcttcaa
     2761 ttattttctt cctaaggctt agtggttcaa ttacttgtat tgtctttcta taaggtaaaa
     2821 gtatatttgg tatgtacttg catattactt cctcttcaag cataaatatt attttattag
     2881 gtgttctttc tttcaagtag tgccctaaaa accaatgttg gcttaaacta tctaaagaat
     2941 ctatactccc ttctataact aattgaatta tattttgttc atcttttatt tcaggtataa
     3001 ttccttttat aaaaaattca cttgctgaaa attctgtcgg tctgttaaat gtatttgatg
     3061 tttctatgat ttcactaatc ctatccactc tgaaactacg aacctcattt ctcatattac
     3121 aaaatgcaac tacgtaccaa ttattattcc agtgaacaat tccatatgga tttataattc
     3181 tatattttga ttttacttta cgacctgcat gatagttgat ttctacagat agctccttta
     3241 atatactatt ctccaatttt ttaagtactg attctataga cactttccca attggtttta
     3301 ttacttcaag ggctcttaag tggtttgtta aaatatcttc ttgtttttga tttgaataca
     3361 tttttaactt taatatcgat ttatctaatg cttcgtctaa gaagtatcct tcttttttag
     3421 caaatagtgc tgcatgaagt agagattttt gctcttctat atcaaaaaaa agaggtgctt
     3481 tagtaaaatt atttaaaagg ctatacccac cattatgacc tgtatctgaa attataggta
     3541 ctccactcgc agaaagtaca tcaatatatc ggtacactgt tcttatattt atttctaatt
     3601 tttcagctat ctgttttgct gtaatttttt tgtcagagtt taacatccac agtatagaca
     3661 tcatattgtc aattttagcc atgtagtttc acctctttct atttgttata taaagttatt
     3721 atataacttt ttgattgatt ttttacaaaa aaacattagt aaactgctaa taatttatac
     3781 taagatttat ttcaaatatt tatagatagt ctttttctat agttaagtta tccattaaat
     3841 tacatagtag tatatctatt ttagttgata tattttcagc tacctccctt gaatattatt
     3901 acaataagtc taaatcttct ttcttccata aatatattat gaggaaagac tatcatcttt
     3961 aatggtttaa tagtataaaa gatattaatt taaaaaaatc tatttttata tcagatgggt
     4021 attgtttttt ataaaactga aaaaatcacc taatagaatc tatgattttt taacataacc
     4081 tccataaatt agttccaatt atcacttcaa attcaaattt ttatctgaag aatatacaat
     4141 catatttcca tactcaacat aatattgagt gtttggcttg tatttacaaa ttattattct
     4201 aacaaaaaca actatacata gattaaaatc atcttctaaa cataactact aaaattgaat
     4261 taatgatatc aattccatta atctctttta ataaaaaatt ctcttttcta tatgaaaatt
     4321 atttcaaaac ttttaagtta aatacaaaaa tgactataaa tagattttta tttacacaaa
     4381 tctatttata gtcactatag tttatccaat aaaataataa aataaatatg tacacaataa
     4441 atgttattta cgtcttaagt atttatttgt tagattttta attaaaaaaa acaagctaat
     4501 gaacattaat gctaaatcta gcattatatt gctttgatct cctgtttgta cattttgacc
     4561 tacattacta tctaaacttt catctgtatc tatatttata tctggttttc cattcccatc
     4621 tgtatctata tttatatccg gttttccatc tccatctata tctatattta tatctggttt
     4681 tccatctcca tctgtatcta tatttatatc tggttttcca tctccatctg tgtctatatt
     4741 tatatctggt tttccatctc catctgtgtc tatatttata ttcggttttc catccccatc
     4801 tgtatctata tctggtttgc tatttcctcc attatctggg tctggtttac tatttccacc
     4861 gttatctgga tccggtttgc tatttcctcc attatctgga tctggtttac cattgccacc
     4921 gttatctgga tctggtttgc tatttcctcc gttatctgga tccggtttgc tatttcctcc
     4981 attacctgaa tccggtttgc tatttcctcc attacctgaa tctggtttgc tatttcctcc
     5041 attacctgaa tctggtttgc tatttcctcc attatctgga tctggcttat ttagatttgg
     5101 attacctgta tcataacctc ctgagccagt atctacattt ttatcaggct tccaataatc
     5161 atctactcca tcattatctg tatctattgc tggacgatta taatctgtat cagggtttcc
     5221 atctccattg gtatctatat ttttatcagg tttccaaata ccatctccat ttccaccttc
     5281 tgtactaggc ttccattcac ctgtattatc agtatctatg ttaatatctg gcttccaatc
     5341 accactcgta tctatattga tatctggcct tccatcacca tcagtgtcta tatttatatc
     5401 cggtattcca tccccatcta catctacatt tgtcatatct aactttaact tcttttcatc
     5461 ctcactacct tcaatgtaat ttatatcatc tccaatatat tttacagtaa tagtttgttc
     5521 agtagtaaaa atatgatgat tgctatctaa aggtctccat tcaatagatg cagtactata
     5581 attatgactc tcactaccct tattatgagt ttttaattct actggatctt tatattcctt
     5641 tccattaata tagaattgta cgtaacctct tggagaggca caatctggtc cttctttctt
     5701 atctggtgaa atagtaacat ttaatgtaaa cgtatctgtt ggtttaaaaa catcagtttt
     5761 agctttagac tcttctacaa ctaatgaagt ttctgtatca gcaggaataa tttctgcctt
     5821 ataatatgct ctatgccccc aataagcatc cttaaaagga tatatactcg aaaactgtgt
     5881 tgatgtaatg cttaattgat atttcccagc atcaacttgt tttgtaagat catcacttcc
     5941 taaatttata tctggttcca tcgtttcctc atttaaacgt tgagaactaa tagtcataaa
     6001 ttttttatct gtgagttttt cacctttagg agtaattatt ggaataccac catctttaaa
     6061 tatccaatct aaaaatgatt tttctacgtc ttcttcaaat ggtaatccat catatctttt
     6121 aataatcaaa tatggtggag tatcatcaat agtaaattct tcatattgct ttaaaaattg
     6181 agaacctgaa cctatatctt taatataaaa atcatctggt tttagaactt ctccagtaat
     6241 tttatctata acaactaaat taacagatac ttcagatccc ttaaattgat cagagaacca
     6301 taagtaaaga atcccttgag catctgtata actaggtgct ccataaggat aaatttttcc
     6361 atcaattagc attttaaaat catcaactag tgtagtacta actttatctt gccctaaata
     6421 cgttagagat attttattca taaaaacttt tgttttcttt tctaaatcac cgtatgcaac
     6481 tgctccacct aaacttttga atgttgttgc ttttaatgaa ccacctgtta ctataacatc
     6541 tcccgttgta gcacttaggt caaaaatcca atctcccact cctgtcatat aaggtaaaat
     6601 cgttcctcca gttacaagta tacgatatcc tctatttgtt cctggattaa cacatccatc
     6661 cccaaatgca ctaccatgtg cacctccata tgaataggaa agtccaccat taattgtaat
     6721 attcccacta taaccagaac ctgtgctagg atctactact gagggactta atgcattctt
     6781 tgttgttgta tagtatcctc cacctattcc agctccatat cctgtattac gactcgattc
     6841 ttgacctata gcagttatag caccaccatt aataataata tttcctccac tttcagtatt
     6901 tccaccacca atacctgcag aataataatt tccaacaact tttaaacttc ctctattatt
     6961 actatctagt agtgtaaggc gatctccttt ttttaacttg tctccatttt ctagagttac
     7021 atctctagga atttttccac cctcaggaac tataaactct ccttcaatat ctctatttac
     7081 aacagaatca tcaataatta gagtagtacc ttttcctgct ctcatacctg gataatatac
     7141 atcacctgac tctagcttgt tttcactacc atcttgtaat ataacatgtg catatgcacc
     7201 accatcttta tctggtgtta aagtaaatgg gctattagat gcagttttaa tatttactcc
     7261 atcaaatatt aattcagctt ttacatcttt aggtataaca atcctatgtg ttgttggagt
     7321 atttggattg ttattttgaa ttgtcatttg ttttccacta agaatagtta aaacgccact
     7381 tgcaaattta aaatcgacat ttatttctcc accactaatt ttaaagtctc ctacagacgt
     7441 aggctcttct gcagtggctt cttcttctac aactactgca ttagcatctc cttctgcat
//
LOCUS       DAMJZE010000183         7495 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJZE010000183 DAMJZE010000000
VERSION     DAMJZE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.187"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            126..1103
                     /locus_tag="PC541_003277"
     CDS             126..1103
                     /locus_tag="PC541_003277"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2840698.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1096..2064
                     /locus_tag="PC541_003278"
     CDS             1096..2064
                     /locus_tag="PC541_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2840699.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2247..2837
                     /locus_tag="PC541_003279"
     CDS             2247..2837
                     /locus_tag="PC541_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2840700.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2856..3626
                     /locus_tag="PC541_003280"
     CDS             2856..3626
                     /locus_tag="PC541_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2840701.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3763..4731
                     /gene="dusB"
                     /locus_tag="PC541_003281"
     CDS             3763..4731
                     /gene="dusB"
                     /locus_tag="PC541_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2840702.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4840..5319
                     /gene="greA"
                     /locus_tag="PC541_003282"
     CDS             4840..5319
                     /gene="greA"
                     /locus_tag="PC541_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2840703.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5334..6863
                     /gene="lysS"
                     /locus_tag="PC541_003283"
     CDS             5334..6863
                     /gene="lysS"
                     /locus_tag="PC541_003283"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2840704.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7172..7366
                     /locus_tag="PC541_003284"
     CDS             7172..7366
                     /locus_tag="PC541_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840705.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2997 a          758 c         1510 g         2230 t
ORIGIN      
        1 aaaacaacca ttataataat taaaaaggaa atttaaaatt aatagtatta aaagtatatt
       61 aataatgtat aattatttat tagaagaaaa taagtcatga aattatgtca tattagaggt
      121 gaaatatgag agataaaata atagagatta tattaaataa taaatctgaa tttatttcag
      181 gagaagaatt atcaaaacaa ttaggggtgt cacgtgcagc tatatggaaa catatgaaag
      241 ccttaaaaga agagggatat aatatagaat ctgtaaataa aaaagggtat cgattagcgg
      301 aaaatccaac tgatttatta agcccacaga atatttacta taaattagat acagagttta
      361 ttggaaaaaa tattgaacat cttgacacta tagattcaac aaatgaacat gctaaaagga
      421 tagctataaa tagtgtagat gggactgtga ttttaagtga acatcaagat atggggaaag
      481 gacgattggg aagaagatgg gattcaaaac ctcatgaagg aatctggatg agtattatat
      541 taaaaccaga tatggaacca ttcaaagccc catttataac tttaatagca ggagcaagtg
      601 taacgaaagc tttaaacaat ctaggagcaa atacattaat aaaatggcca aatgatgtta
      661 ttttaaatgg aaaaaaaata tgtggtatat taacagagtt atctgctgaa attgaaagaa
      721 caaattatgt tgttcttgga attggaataa atgttaaaat aatggatttt gaacaagaaa
      781 tagctgacaa agccacatca ttgtataaag aaggatatat gttatccaga gtagatattg
      841 ttaggaatgt attaactgaa tttgaaaaat tatatttaga ttatactgta aataattcca
      901 aagaaaaaac cttaaaaata tgtagagatt attctgcaat tatagataaa aatatatatg
      961 tattaaaagg agataataga gaactagtga aatgtttaga cataaatgaa gagggaaatt
     1021 tattagttca aagaagtgat ggaagtataa aagaaattat ttcaggtgaa gtttctataa
     1081 gaggagaaaa aggatatgta taataatatt ttggatatag aaggcttaaa ggttgggcag
     1141 gttgaggata gaaatggtct tactgggtgc acagtagtaa tatgtgaaga aggagctact
     1201 tgcggtgttg atgtaagggg ctctgctcca ggtactagag aaaccgattt attagaccct
     1261 gtaaatatgg ttcaaaaagt tcatgcagta gttttggctg gaggttctgc atatggtctt
     1321 gaatccactt gtggtgtaag tagatactta gaagaaaaag gaattggctt tgatgtaggt
     1381 gtaggtaaag tgcctatagt tgtaggagca gttttatttg atttaggagt agggagcttt
     1441 aaatgtagac cagatttaaa gatgggttat agagcttgtg aagttgcaaa tactgatatt
     1501 ttaaatcaag gaaattatgg agcaggttgt ggagccactg ttggaaagat aagaggtcag
     1561 gaatatgcta tgaaaggcgg aataggtagc tattctataa aattagataa tggtctaatt
     1621 gtatctgcat taattgctgt aaatgctctt ggagatgttt atgaaaatgg taaggttata
     1681 gctggtgctt taaatgataa aaagacagaa acaattgata catataaatt aatgaaacaa
     1741 ggtgaaagta aaggtggatt tagtgtagat aatactacaa ttgggataat tgcaacaaat
     1801 gctaagttaa ccaaagctgg atgtaaaaag atatctcaaa tgggacatga tggatatgct
     1861 aagtctattt ttcctatcca tacacctcat gatggagata caatattcac aatatcaaca
     1921 ggagaaatag aagcagatat aacattagta ggttcattgg ctgtagaggt tgtggaaaaa
     1981 agtataataa atgcagtaaa aaaagcggat agtgtagaaa atataatggc atataaagat
     2041 atagtaaaaa aacgagaaat atgatgaaat tagcatattt ttcttgtcaa attgttgaca
     2101 atggcaactc atcttgataa aattattctt gttagagcgg gcatcctaca atggtagctt
     2161 gactcatagt tataagaaaa aacgaaaaaa attaaaaaat taagtagtcc ggcatcttaa
     2221 tgagctggaa cggaggtgta ttttttatga tgaacacaag tgcatcagta agaaaagtaa
     2281 atgtaagaaa gatgactata ataggagtac tatcagctat ttctattatg ctatctatga
     2341 ctccattggg atttattcca gttggaccta caaaagcaac tataatgcac ataccagtta
     2401 ttattggagc tattatggaa ggaccaatag ttggggcagc aataggattt atatttggga
     2461 tatcaagtct cttaaatgct ataacaaacc caacagcaac atcttttgtt tttataaatc
     2521 cattagtttc aatattacca agagttatga taggtgtatt agcttattat gtatatcagt
     2581 taataattaa ggctacaaat aaggtataca tatcaggttt aattacaggg gcaataggtt
     2641 ctctgttaaa tacagctgga gttttaggaa tgatatatgt cttatatgct gacaagtatc
     2701 ttcatgcaat gggtaaaagt ggttcagcag gaaaagtaat aatggctctt gccgcagcaa
     2761 atggggtgcc agaagcaata gtaggggctt tagtagtagc tgctgttgcc actgtactta
     2821 agaaaagcaa aaaatagaaa gaaggttaat gacatatgct tctagtattt gatgttggaa
     2881 atactaatat ggttttaggt atatataaag gtgacaaatt agttaattac tggagaatta
     2941 aaacagatag ggaaaaaacg tctgatgaat atggaatcct gataagtaac ctatttgatt
     3001 atgataatgt gaatataagt gatattgatg atgttataat atcatctgta gttccgaatg
     3061 ttatgcattc tcttgaaaac ttttgtataa agtactgtaa aaaacagcca ttaatagtag
     3121 gtccaggcat aaaaacaggt ctaaatataa aatatgataa tccaaaacaa gttggggcag
     3181 atagaatagt taatgctgta gcagggatag aaaagtatgg agcaccaagt atacttgttg
     3241 attttggaac agcaactaca ttttgtgcta tctctgaaaa aggtgaatat ttgggtggaa
     3301 caatagcacc aggaataaaa atatctagtg aggcgttatt tcaaagtgcg tctaaattac
     3361 ctagagtaga attagctaag ccaggtatga ctatttgtaa gagtactgta tcagccatgc
     3421 aatctggaat aatttatgga tatgttggtt tagttgacaa aataataagt atgatgaaga
     3481 aagaattgaa ttgtgatgat gttaaggtta tagctacagg tggattagct aaactgattg
     3541 cttcagagac gaaaagtata gattatgtag atggtttttt aacactagaa ggattgagaa
     3601 taatatatga aaaaaaccaa gaataattaa tatacaggga attgggttat atatccaatt
     3661 ccctttttat gatagatttc tagctatatg tggtatatta taatgaggaa ataagaattt
     3721 aaagttaaat cacaaaataa aggcaaaaat aggagaaaaa caatgaaaat aggaaattta
     3781 gaattgaaaa ataaagtttt tctatcacct atggcaggtg taacagacct tccatttaga
     3841 ttaatttgta aagaacaagg atgtgggctt ttatatacag aaatgataaa tggtaaggca
     3901 ctttgctatg atgatgaaaa tactaaaaaa atgcttaaga tagaagagga agaacatcct
     3961 gtagcagttc agatattcgg ttcagaacca gagtttatgg gaagagcaac agagataatg
     4021 aatgattatt caaatgagat tctcgatata aatatggggt gtccagctcc aaaagtagtt
     4081 aaaaatggag atggttcagc acttatgaaa aatcctaagc ttgcagaaga ggtattgagg
     4141 gcagtagtga agaactcaaa aaagccagtg actttaaaga ttcgaaaagg ctgggatgat
     4201 aatagtgtaa atgctgttga aatagcaaag atagcagagg attgtggaat aagtgcttta
     4261 gcaatacatg gaagaactag agaacagttt tatacaggca aagcagactg ggatataatt
     4321 gcagagatta agaaaaattt gagtatacct gttataggaa atggtgatgt atttacaata
     4381 gaagattcaa taaatatgtt agacaaaact ggttgtgatg ctataatgat aggtaggggt
     4441 gctcaaggta acccttggat atttaaaaga ataaaccatt atatgaatac aggcgagatt
     4501 ttgccagagc caactcttaa cgaaaaaata agtactgcca ttaagcattt aaaacttgca
     4561 gtagaagaac atggagagta tgtagctgta agggaaatga gaaaacacat cgcttggtat
     4621 ttaaaaggac ttagaaactc tgcaaaatta agggatgaaa ttaacaaaat agaagactat
     4681 caagaagttg tgtcgaaatt ggagtactat atggaagact ccttgacata aaaataatag
     4741 tgacttataa tatagcgcat aaattaaaaa aagattataa aatttttaaa atataattag
     4801 gaaaattcct aagaaatttg tgaaggagtt gtatttataa tggaagaaaa taaagagttt
     4861 ttattgacac aagagggata tgataaatta gaagaagaat tagaaaactt aaaggttgta
     4921 aagagaaaag aagttgcaga gagaataaaa gttgctatat cttttggtga tttatctgaa
     4981 aatgctgagt atgatgaagc taaaaaggaa caagcacaag ttgaagaaag aattttaaaa
     5041 ttagaaaata tggttagaaa agctgttatc atagatgaaa gcaaaataga ccttaatgtt
     5101 gttacaatag gatcaatagt taaagttaag gatttagaat ttgatgaaga ggttgaatat
     5161 actatagttg gttcgacaga agcagaccca tatgatggta aaatatcaaa cgaatctcct
     5221 gtagggaaag cacttttagg tagagctgcg aaagaagttg tggaagtgca agttcctgat
     5281 ggagttgcta agtttgaaat attagaaata agaagataat ttggaggaaa aaaatgaaaa
     5341 ataatcaaca aagcaatgaa gaagcgcaaa ttcaagaaga ccttagtgaa gtacttcaag
     5401 taagaagaga taaactaaaa aaattacaag agtcaggtag agatcctttt aaagaaagta
     5461 gatatgatag aactcattat tctatggata taaaagataa ctttgattct cttgagggta
     5521 aaactacaaa aatagcagga cgtataatga gcaagagaat acaagggaaa gctggcttta
     5581 tagatattca agaccaagag ggaagaatcc aatcttatgt aagactagat gctataggag
     5641 aggaagaata tagtgtattt tcaacatatg atattggaga tatagttgga attgaaggag
     5701 aaatctttaa aactaaaaaa ggtgaaatat ctgtaaaggc taaatctgta gtattactat
     5761 gtaaatcttt acaagtttta ccagaaaaat atcatggatt aaaagaccaa gaactaagat
     5821 atagacaaag atatgttgac ttaatagtaa atccagaagt aaaaaatgct tttttaatta
     5881 gaacaaaagc attaaaagca ttaagagcat acttagatga tagaggattt ttagaagttg
     5941 agactccaat attaaatact atagcaggtg gggcaaatgc tagaccattt ataactaatc
     6001 ataatacttt acatatacca atgtatttaa gaatagcgaa tgaattgtat ttaaaaagac
     6061 ttattgttgg tggatttgat aaagtttatg aaatgggaag aatgtttaga aatgaaggta
     6121 tggatttaaa acataatcct gaatatactg caatagaatt atatcaagca tatgcagact
     6181 atactgatat gatggaaata acagaaaatg ttattgcaca tatggctgaa gttgctacag
     6241 gaagtatgat agttaactat caaggaacag aaataaactt tacaccacct tggaaaagaa
     6301 tgagtatgga agattgtgtt aaagaatatt ctggtgtaga tttttctact ataaatacag
     6361 atgaagaggc actagaagtt gctagagaaa aaggtataga gataaaacct ggaatgagaa
     6421 gaggagaagt aataaatgct ttctttgaag aatttggaga agataaatta atccaaccaa
     6481 catttataac tcaccatcca gttgaagtat ctccattatc taagagaaat gttgaagacc
     6541 caagaagaac tgatagattt gaggcatttg ctaataagtg ggagttagct aatgcatttt
     6601 ctgaacttaa tgaccctatt gaccaaaaag gaagatttat agaccaattg agaaaaagag
     6661 aattaggtga tgatgaagct tttgaaatgg atgaagattt cttaaaagca ttagaggtag
     6721 gtctacctcc tacaggagga ctaggaatag gtatagatag agtaattatg ttattaacta
     6781 actctccatc tataagagat gtattattat tcccgactat gaaacctatt gataacaatc
     6841 caaataaaga agaagaaaac taagaaatat tagcattcta ctaagtttta aataaagttt
     6901 agctattaaa ttatcaataa atagctaaat tttatttaaa atgtcagtgt ggaatgcttt
     6961 ttatatgctt caaagattct tttagatatt taatttataa attaccaaca aacgttagaa
     7021 attgatatta gtttttgttg attgagactt tatatttaga aaatatgatt gaaagtaatt
     7081 tattatgaaa atatttagaa taatttatga gaaggtaaaa agaataattt atgagaatac
     7141 tatatataaa aaattattgg gggaatgtct gatgtttcaa gtaattaatt ttttatgttt
     7201 attttgtgtt ggatttttgg ttcttgatgt tatgatatat ttttgtaagg atgcaatgga
     7261 tatttttgag ggtacaacta atgttaataa agttaagagt ataaatgttt gggcagatag
     7321 agtgagagct aattcatatt cttatgaaaa taaaattgca aaataggtaa aattatatat
     7381 gcatgtattg tttgtaatat caagggttat atctattagt tttaattata tttattaaaa
     7441 aaaccaaaag ttataacctt ttttaaaata aaaaacaaaa aaagtattga cgaaa
//
LOCUS       DAMJZE010000184         7467 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJZE010000184 DAMJZE010000000
VERSION     DAMJZE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.188"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(162..557)
                     /locus_tag="PC541_003285"
     CDS             complement(162..557)
                     /locus_tag="PC541_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2840706.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            698..1090
                     /locus_tag="PC541_003286"
     CDS             698..1090
                     /locus_tag="PC541_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840707.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1190..1606)
                     /locus_tag="PC541_003287"
     CDS             complement(1190..1606)
                     /locus_tag="PC541_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2840708.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1621..2532)
                     /locus_tag="PC541_003288"
     CDS             complement(1621..2532)
                     /locus_tag="PC541_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2840709.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2714..3736)
                     /gene="splB"
                     /locus_tag="PC541_003289"
     CDS             complement(2714..3736)
                     /gene="splB"
                     /locus_tag="PC541_003289"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2840710.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            complement(3963..5093)
                     /locus_tag="PC541_003290"
     CDS             complement(3963..5093)
                     /locus_tag="PC541_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2840711.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(5481..7418)
                     /gene="htpG"
                     /locus_tag="PC541_003291"
     CDS             complement(5481..7418)
                     /gene="htpG"
                     /locus_tag="PC541_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2840712.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         2431 a         1294 c          781 g         2961 t
ORIGIN      
        1 tattcaaaat agtatatcat tattgaaata cacatctaat aactaaaaat ttttacatat
       61 tcattagtta aaaatctttt tattgcattt aaattaaatc tatcaatatt tcagtaataa
      121 cgttttctta attttcgcta tggatgtcga ttcaattata attaaagctc tacatcaatc
      181 tttttaccac tcattgaaca acaactaact tttgccttat caatagagaa tatttcaaac
      241 ttaccatctc ctacagagta catctgacct aaacttggca ttatttcaag tgccttagct
      301 tgtgtttctt ttgatgtaac aaatactgct tcacctaaaa tacgtagagt attaaggttt
      361 tggtctatac aacaaagttc aacctttgga ttaaaaataa gttgcttaaa catatccttt
      421 tgattagatg tgcaaaatgt aagtttacca ttgtattcca taacaaatcc taaaggacgc
      481 acatgtgctt gattgttctc atcaactgtt gctaaataat aaactcctgc cttgttcaaa
      541 aattctaatg ctttcataat tcttacaccc cttttaaata cttgtattaa ggattatcct
      601 ttgatataat tttaatataa aatacagaaa aaacaagtat gcagtttttt ctgataaagt
      661 ataaaaaaga tactactata actaaggagt aattaaaatg agtataaata ctgaaaaaaa
      721 tataactaaa aacgttgaag aattaacctg tccaatccgt tatgcattag acattgtcgg
      781 tggtaagtgg aaattaccta ttatatgtat gttagcagtt gaaaatccaa ttagatatag
      841 tagcataaaa agaaaattgg atggcattac aaatactatg ctagctcaat cattaaaaga
      901 tttagagtct actggtattg ttcatcgtaa acaatataat gaaattcctc caaaagttga
      961 atatacatta acaagtaaag gtaaaagtat tgtgcctatt ttacaacaat ttgctaattg
     1021 gggtgctact aatatgcaag aaaaaaacac atgtggactt agttgcaaag aatgtagaaa
     1081 gataaagtaa atttaaattt actaaattat tcatattata gactatatat taatttatct
     1141 gattgaatat aaaaacagga tgacttatat attcatcctg ttctatatgt taagctctaa
     1201 atttaattag ttcaagcata tcgtttaata ccaataaact atctacttga atcattagcc
     1261 atttacttcc attaaaaaag tttacctcat tatatatttt tttgatataa tcacaacaag
     1321 tattaataaa taaatcaacc tctactctat tgctttcact tacaataacc aaagctttaa
     1381 aataaccttc ttgtggatat aaagtacata gtgatttacc tcttttttta tattttacat
     1441 tccaacctct ttgcattgaa catttactgt attcaagctt aggttctaca ttataagtag
     1501 aaaccaaagt tttatttaac ttgtcccata atggattatt aataaattca ttaatttgct
     1561 caaatgtagg tttagtatct tgaaaaaacg catcttgcca attcatattc atcatccttt
     1621 ctattgttca ataggaaccg gaacaatcca atgttccaga tatgctgctt caggtgtagt
     1681 ggggtaatac atctcaattg caaaatcagt agttgttaaa ttatgtttct tcatccatct
     1741 ttccataaat ttatctgctt taaaaacagc tgaattaact aactcattaa agttttctgc
     1801 ctcaaatcca catactaaat attctttagc tggcaactca aaagtactat atccttctac
     1861 tgatgtacct gactctacct ctacacctgc catatacata caatttcctt cttttgcatt
     1921 tcccatataa agagcagcaa attcatttcc acctggaatt tgatttggta tattatgtct
     1981 ttgacttcca agtttcatcc ataattcctc tactattgca attccagttg tttcaccgcc
     2041 caccaactgg caaataggta cttctatttc tattccaaca aatgtccttg gattactcaa
     2101 tgtcttacga ttgacttcaa tcactatatc atctgcaatc agtggaacac cttcatcaac
     2161 catagcataa tttaaaatta attctggctt aataaaatga ttgagtttta ctggatttgc
     2221 tctatatttt tctggagtta atccatatgc ttttttaaag gaacgtgtaa atgtctcatg
     2281 actagcaaat ccaaaattta aagctacatc aagtatacga ttcttatgtt ctgctaagta
     2341 ttcagaagct cttgcaagac gacgaagctt tatatactcc attacagtct tatttaccaa
     2401 tcttttaaat agtctttgat agtaaaatgg tgataaattt gctttatttg ctaattcatt
     2461 aattgatatt tcttctgata aatttgcttc tattaaatcc aatgtaattt gaatagactc
     2521 ccatgcatgc atactataac tcctcctgat atttttgtta ctcttatcct ttatagtatc
     2581 aaaactagaa aaaatgttct tgatagatag tgcccttttt aattttctta tatgataatg
     2641 ctaaatctca ttgctttatt ataaacataa aatatataaa aataaaatat agcaaatctt
     2701 taggtaagat ttgctatatt atatatttta ttaaactatt tttaaatact ccatttattt
     2761 ccttaagaaa gaactctttc atatcatcta attgttcttt atcatacacg tacttaccat
     2821 atccaaattg accgtattta aactttctat cttcattttc cattggcaat agtgtatctg
     2881 gataaatctc tagtattcta ttttttgctt tagttgtata tctatgagat ataacttcaa
     2941 atataatttt tttatcaaaa tcctttggta aagcttcttt tatacttata atcagatttt
     3001 tatactcttc tttccaacct tcatataaaa acacaggtgc tattatgaaa ccaatctgat
     3061 aaccagccct agcaagtttt acagctgact ctattctttt atttgcagag gctgtataat
     3121 gctcatgttc atttataatt ctaggagtat tgatactaaa tcgtatttct gtatgattat
     3181 tatgttcagc atccaataag ctatccacat cactgtattt agtaacaaat ctaaatcttc
     3241 ctttttcctc tcttccaaaa aactctattg ttttcttcaa agaattagta tatggctcta
     3301 ctggaactgg gtctgatgta gctgcccctt caaatatggt tacctgtggc tttctatctt
     3361 caatatattt ttttgtctgt tcaagtattt catctacatt tacaaacacc cttataaatg
     3421 gtttatcacc taattgtgtg ttcaaataac aatactcaca tcttcccata catccactga
     3481 caagtggtag ttgataatta gcagaaggtt tacatgtttg aaacttcaga ctttttctaa
     3541 tcccaactac catagtctgc ttcccacttc tatattgttc atatagattt tcacctggaa
     3601 tatgttcttt aatcctattt gatgatatat taattatctc tacatcttta ttatctctaa
     3661 atttattgta tattttttta cccacatcag tgtctaaagt tcctttttcg aatattattc
     3721 ttttgggatt aaacatttat ttcacctcta cttaaagttt ttaaatcgaa gaattttata
     3781 aaatattact taattaatct tccctttttt aaactccttt atttattaga aagtaaaata
     3841 aaagcttaag acaaatttat tatatatata acttctatac acaatttttt tactattaat
     3901 tacttataat tttttattat taatttataa tttttatata aaactaaata tgtaaagtca
     3961 aattataaag acttatggta catttccttt atttgttcct tattaaaata ctttggatgt
     4021 ctcttaatat ttgcaactga tattttcata caattatcag ttaaccaatc tatatctgat
     4081 ttttcaatac ctaaatcact tagctttata tctaaatcta tttttttaag gaaatttcta
     4141 attgcatctg aacaatcttc tgcacaagtt cctcctaata cctttgaaat attaccaaat
     4201 ctttcaatat cactatccca tgaactttct ataatagtag gagtcaaagc tgcaagtcct
     4261 tttccatgca ttacatctct aagaccactt acaggatgct ccattccatg tggcaaggct
     4321 gtacccgctg taccaatcgt cattcctcca atagtacttg caagtgttat tttttcccat
     4381 gcttctaaat cattaacatc attatatacc tttattaggt tttctgatat aagttcaatc
     4441 ccataaatag cttgtacatc tgcaagaggg tttcttccat tagaaagata tgcttccata
     4501 ttatgggcaa gtgcatcaaa tccaactgat gcaattatat gttttggcat tgtaaccata
     4561 agttctgggt caattattga tgcttttgca tacatactat tcttctttat agatttttta
     4621 tctttagttt ctgaatttgt aagtactgca aaacaatttc cttcactacc tgtacctgat
     4681 gttgttggaa ctaagattat aggtaaagct tcttcacctt gtctaattcc aaatatataa
     4741 tcatttaaat ctcctgggtt tttataagaa aaagctatac tcttggcagc atccataatg
     4801 cttccaccac caagacctaa aacacaatca catccagttg attttataat ttctactcct
     4861 gaatatatag tagttataag aggattttgt tctactctat caaaaacttc atattctatt
     4921 ttagagtctt ttagtaaatc tattgtttta tcaagtaatc cagttttctt tgtactattt
     4981 ttacctgtta ctatcaatac ttttttgcca tatttagata cttcttttcc cacatcatta
     5041 attctgccgc tcccaaataa gatatttact ggcaaattat aattaaaatt catgctaaaa
     5101 cctcctattt ctactttagt aataatttta tgtttttaac aaagtgtaat tatgtattta
     5161 aacataaact aaaaaacaat cttttactta attatatgta ttatcttatt atatatcaag
     5221 ttaactatat ttaactaaat agagtttttt attagaacct acttaaatat ttaaaaatta
     5281 accaaagata aaaaaataaa gaacggtctg tctcaaaata gagataaata tatttttcat
     5341 tgttgaaatg attaaaataa atttaatttc acataaaaaa agcgcctcat gaactgaaaa
     5401 gttcattttg agacacttct ttcttatcac tgatattgaa tactttttat aaatttgctt
     5461 caatgtattc aaattcaatc ttataattct atcaaaagac tcataagctt attacttctt
     5521 tgtacaaatg aatctaactc atctggcttt aattctttat tagaaagtaa agctaaatct
     5581 gctatctggt tacaaataag cgtaatctta tctttctttt cctcatcatc tttaagagaa
     5641 accaattttt tgattattgg actgttctca tttattacaa gagttttttc ttcttcaaaa
     5701 ttcattccta aatccattcc agcaaatctt gactgcattt ctgccattct aatagattgt
     5761 tctgatacta acaccatagc tggagtgtct tcatttttca atccttctac agaataattc
     5821 ttcactcttt ctccaacagc ttctttaaac agaccttcta tcttagctat ctcttctttg
     5881 ttttcttcac tatctttatt ttcattttta tcttttaata catcagaaag gtctgcatct
     5941 attctattaa acttaactcc attctcttta tattccataa atgaaataaa atgagtatct
     6001 attgatgagt ttaaaacaac agcacttaag tcgtattctt taaatagttt aatgtattga
     6061 gattgctgtt ctttatcact tacataaaat accttatttt catgtttgtc cttacacttt
     6121 tctaaataat cttgtaatgt tatatactca tcatcaatag ttttgaataa tatacattct
     6181 tttaccttct catagaaact ttcatctttt aagcaaccat atttaataaa tatttgtata
     6241 tcatcccaaa acttattgta ttcttctctt tcatttttac aaagagattt taacttatca
     6301 gctacttttt taattatatg tttagaaatt ttgcttacat ccctgtcatt ttgtaagaaa
     6361 cttcttgata catttaatgg taagtctggg cagtctataa ctccttttaa taaaagtaaa
     6421 aattctggta taacttcttt tatattgtca gccacaaaca cttgattgtt gtataatttt
     6481 acttttcctt cagtcagttc aaactcattt tttaatttag ggaagtaaag tatacccttt
     6541 aaattgaaag gataatctac atttaaatgt atccaaaata aaggttcatc aaatacattg
     6601 aatacctttc tataaaactc tttatactcc tcatcagtac agtcttttgg agattttaac
     6661 catagaggat tagtgtcgtt taaaggctca attacttttg catctacaat ctcttctttt
     6721 ccatcttcct tttgtttttt agctttttct tctgcttctt tagcttctct ttctaatctt
     6781 tcaacatctt ctaaatatat ttctactggt aagaaagaac agtatttatt tattattcct
     6841 cttacagtaa attcatctaa aaattcttta ctatcatcat caataaatag tgttatagta
     6901 gttcctctat catttctagc atctgattca gatatttcgt attctgtacc accttcagat
     6961 atccatctta caggagtcgc tccttctgta tatgaaagtg tatctatttg taccttttta
     7021 gacaccataa atgcagaata aaatcctaat ccaaaatgac ctattatatc atttgactct
     7081 tccattttat ccttatattt attaaagaaa tcttcagcac ctgaaaaagc aacttgattt
     7141 atgtattttt ttatctcttc ttcagtcata ccaattccat tgtcaataaa tttaagtgta
     7201 ccttctcctt tatttacaga tactgtaatt ttataatctg atgacttatt ttctgatatt
     7261 tctcctagtg atacaagtct tttatgttta cttactgcat cacatccatt acttataagc
     7321 tctcttataa atatgtcttt atcagagtat aaccattttt taattatagg aaaaatattt
     7381 tctgtatgaa ttgaaatact ccctttttca aattccattt tttatccctc ctcattttat
     7441 aattaatatt ttaaattttt taaaatg
//
LOCUS       DAMJZE010000185         7418 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJZE010000185 DAMJZE010000000
VERSION     DAMJZE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.189"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            445..1137
                     /locus_tag="PC541_003292"
     CDS             445..1137
                     /locus_tag="PC541_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840713.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1333..3393
                     /locus_tag="PC541_003293"
     CDS             1333..3393
                     /locus_tag="PC541_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2840714.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3598..3921)
                     /locus_tag="PC541_003294"
     CDS             complement(3598..3921)
                     /locus_tag="PC541_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2840715.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3914..4615)
                     /locus_tag="PC541_003295"
     CDS             complement(3914..4615)
                     /locus_tag="PC541_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2840716.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4893..6278)
                     /locus_tag="PC541_003296"
     CDS             complement(4893..6278)
                     /locus_tag="PC541_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2840717.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6386..>7418)
                     /locus_tag="PC541_003297"
     CDS             complement(6386..>7418)
                     /locus_tag="PC541_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2840718.1"
                     /translation="HNIDNYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYNYI
                     ENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYN
                     NKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFHELCELSCDEIV
                     IDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRLDSMFNQDKKKI
                     NLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIE
                     PELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGI
                     KIIGRFEIN"
BASE COUNT         2851 a          850 c         1049 g         2668 t
ORIGIN      
        1 ataaaaatag aatttaaata agtagctatt attgcagtga aatgaatgtt tctaagtata
       61 tttgggtgga agaatggtgg aagaaaatta gtgaaatgta attcttccac cattcttcca
      121 cctagaaaat agaaactgtt taataagaat taatatacat taattcagct gaatgcaatc
      181 aaaatactta aatgaaagta atctgaaaac aacataattc aaatcaattt attcaaataa
      241 acctcaattt atcttaaatc ttaagaaata tttaagaatt aggataatat tttcttaaga
      301 tttatatttt attatatagt tataagggaa agttacaaaa atgtaagcca aagtaatata
      361 acaatttagt cattgttgag aaagggagtg aaagatattg tataatatag ataagtatta
      421 ttatacaata gaggaagtga aaatatgtac aatatattag tagtagacga tgataaagaa
      481 attgttgatt cgatagaaat atatcttaaa tcagaaggat ttaaaatata taaagcatat
      541 gatggaatga aagctttgga aataataaca gagaatgaca ttcatctaat actaatggat
      601 ataatgatgc ctaaattaga tggaatcaaa gcaactgtaa aaatacgaga agaaagaaat
      661 attccaataa ttttaatttc agcaaaaagt gaagatacag ataaaataat aggattaaat
      721 attggagctg atgattatat aacaaagcca tttaatctat tagagcttat agctagggtt
      781 aaatcaaact taagacgata tgtaacttta ggtaattatg aagatacatg taaagaaatt
      841 ctaaaaagtg gagctttgga gttaaataca ctaacaaaag aagttaaaat tgatggtgaa
      901 aacataaaaa tgactccaat tgagtataaa ataattgaac ttcttttaga aaataaagga
      961 cgagttttct caatagatga gatatacgaa aaagtatgga atgaggaaag ttttaattca
     1021 gaaaatacag tagcagtaca tatcagacga ataagagaaa agattgaaat aaatccaaaa
     1081 gaaccaagat ttttaaaggt ggtgtgggga gttggttata agattgaaaa attttagtgg
     1141 aaaaattata aaagaaaaga gtttaatatt taattgcatt ttgattttag tcatttttac
     1201 atttacacaa atgttaggaa ttttaatatg ttttaatttt ttctctgaga gtttaattta
     1261 ctctatgggg attatgcttt tcttaaactt tattttatat aaaatactga caatgtagat
     1321 gaggtgggca acttggagaa atatagagta ttaaagttag tttcattaat cataatatct
     1381 atctcatttc cattaactat attgttggtg acaataggac ttgctgagcc taaatacaat
     1441 atacaaaaaa cttctacaaa gagtgggatt tttgatatat ctgatgattt ttatgatagt
     1501 aatatatttg acgaaggaac aatggcaagt ctattaccca agtttaagct atctgtaggt
     1561 ggtaaattaa ctagtaaaga aaaaggtaaa ttccaacaag aaggtaaaag tatagattta
     1621 agtagtagtg attctgagta tgataaaatc atgattaatg aacaagcttc tgaagaatat
     1681 ttaagttatc taaaaaatat aaaagtgtat gttattaata catacacagg agtattttat
     1741 acaaatacgt catatgctag tttatatgat tttaaacaaa aaactaagag ctattgtaat
     1801 gtagagataa taaataagat aggaaagagc tcatatatta aaacaataaa tggagaaaaa
     1861 tttgaactga ataatctgag tctggaacta aatgaagtag atgaaggttt tgaggcatat
     1921 gtatcttttc cagaagagcc aactatagaa gatggaatag tatacactaa ttttcaaata
     1981 tttaaacaag ctacagaaaa agtaagatta ttttttgtag ctagtttaag tttagcaatt
     2041 atatgtttat tatctataat tttatatatc aaaataaaag gtgaagtttt aaaaagaaat
     2101 aatgtatttt tactaatata cagtaagata cctatggaat ttaatgtaat attagtatct
     2161 atttcaattg caattttaac tatatatgct cttgctgtat cttatcatat tgatgtaatt
     2221 gcattagttt ctttatcttt actgatagta tcaaataata tattttttat aaatgtgcaa
     2281 ttgagctatt tagaaaataa aatgtgtata tttaagacaa gtattatagt aagagtgatt
     2341 ttatggttaa taaaaactgt aagggaggtc agtaacgcta taaagaaagt atctttagca
     2401 aggaaagtta ttgttatagt gataatatct ttagctttaa tcatggcaat tagcactcga
     2461 agtataggta tgggattact gttttctata tgtatttttt taggatttac aatatatata
     2521 actaaaagac tttcatatct tagttacgta atagaaggta cagaaagaat aaaaaaggga
     2581 gaattagatt ataagataaa aattactggg aatgataatt ttacgtcttt agctgaaaat
     2641 ataaataata ttggagaagg tcttgataga gctatagaag agcaagtaaa aagtgaaaga
     2701 atgaagtctg accttataac aaatgtatct catgatttga aaactccact tacttctata
     2761 ataaattatg tagatttaat taaaaaagaa gaatcaatac aaccagagta tataaatgat
     2821 tatataaatg tacttgagtc aaagtcaaaa agactaaaat tattgataga agatttattt
     2881 gaagcgagta gagtaagtag tggcaatata gaattacaaa ttagcaagat tgatttagtg
     2941 caactattaa gacaaagtat aggtgaactt gaagaaaaat tatctaaaaa taatttatat
     3001 ttaaaattaa atgttccaaa tgataaggtg tatatatggg cagatggaag aagaatgtat
     3061 agagtatttg aaaatctttt atctaatata gcaaaatatt ccttagaaga aactagagtc
     3121 tatattgatg tatatgacta tggtgaaaat gtaaaggtaa ctatgaaaaa tatatcttct
     3181 tatgaattaa attttgaccc aagtgagatt atggaaagat ttaaaagagc tgatgaatca
     3241 aggaatactg aaggaagtgg attagggctt gctattgcaa gagatttagt agctttacaa
     3301 ggtggaaaat tttatataga aatagatgga gatttattta aagcaaattt agagtttcaa
     3361 aaatatgaag aagaaataac aaatatggaa tgagatagta tttaagtata tatatttttt
     3421 tactgtcgaa agcttttatt ataatatatt agaatattta cagtagagaa tgaagagtaa
     3481 aaacagagta actttaagtc actctgtttt ttatttaaaa tcaataatat aacaacttaa
     3541 attaaaattg tacacatagt tttaaaataa cttatacaat aaaacattat ccaaatatca
     3601 caagataatt ccaaataaat atatagatat cactcctgta ataatagaaa taccaataga
     3661 tttaaactta tatgcaacaa gaagtacaat cataaaagat ataattttag ggttagctat
     3721 agataaatat agactatctc cattaaaaaa tacatcttta gcagttaagg aagtaagtat
     3781 tgctacaggt ataaatttca tgattttttt caaaaaatca ggcatctctt tttttgaaaa
     3841 aattaacata ggaagcactc ttggtagata tgtaacaatg tacattccaa ttataataaa
     3901 aaatatataa ttattattca taagattcct cctttttaca gtaagattct tctataaaga
     3961 aaccaattaa agatgctaaa attgatgcaa ctattattac taaattgctt ttgaataatg
     4021 catataaaat aacagaaata atgattgatg atataaatac tacaatataa atcttatttt
     4081 ttatttgaga aactattaaa gctataaaca tagaagtcaa tatgaagcca gaaatagaac
     4141 tgtctataga tatactttct cctaaaaatg ctccaataaa attagctaaa actgatgaaa
     4201 ttaagcagaa gaaattaaga taaatagcat ttctatcaga ccaatttcca gcagtaaatt
     4261 tttcataatt aactgcaaaa gtttcatctg ttatagtatg acaaaatggc aatataaatt
     4321 ttggactttt attttttatg tacatgttca aattagaact cattagaaaa tgtcttaagt
     4381 taactataaa tgtagtcaaa acaatagatg ttacacttgc accagtagaa atcattgaag
     4441 ctgcaatgaa ttgagaactt cctccaaata ctaaagttac cattagtgct atttgaaaag
     4501 gtgaaagacc agatttttgt aatagtattc ctcctactat acctataggt atataaccta
     4561 agaatatagg aattgaagca ataaagcttt cttttaattg ttgagttctt tccacaataa
     4621 atcactcctt ttgtattaaa tgtattaaag tattcgtaaa acagaaaaat agattaattg
     4681 tataattgca attataatta aaaataaatt tggattaata tataaaatac tatacaacaa
     4741 atagataatc ttaaacggtt attaaatttt atctatttgt tattatgatg taataataca
     4801 tattaatctc tataagttaa ttactatctg gtgtaatttt attttaacaa caaaaaaaaa
     4861 tacacacaat taaaatattg tgtgcaatac aattattttt tctcttctaa tagtcttttt
     4921 aatacgctca aaccagcttt aagttcttct gtactttctg tagcacatac agctattctt
     4981 attgcagaaa ttggtttttc ttttccaaca gcaaaacgtt cagaagcata gacctgtaca
     5041 ccttctttaa gtgcttgtat ctcaaatttt tctgccatta ttccatcagg tagaatcaac
     5101 catctaaata tacattcttc atttcccaaa agattatatc cagataaata ttgattaata
     5161 atcttgtttc ttcttcttgc agatttacgg cgtgcattaa ttaatttatc ggcttcacct
     5221 gatgtaatca tgcgataagc aatttctgtt aaaaaataag attgagatag attaatgttg
     5281 tataatgcgc ttgataatgc ctctcgatac tgtttaggtg tagagatata tgctagccgt
     5341 aaactaggtg caataatttt tgataaactc gtaatataaa ttgtttgatt gggcagatag
     5401 gaagccactg gatttaaatg tgtttcatta agtaaactat gaatagcatc ctcaataaca
     5461 attaaattgt atttagatgc aatatttgca atcatttttc gtccattttg tgacattata
     5521 tgtgtagttg gattttgata atctggtatt atgtatagac ctttaatatt ttcattttta
     5581 caagcatata atagtccttc ttcactaatc tcattatctt cttgtttaat tggaattagt
     5641 tgaatcccaa gcattttagc tgctgtcttt attccaggat aagttaatgg gtctacacct
     5701 attctatctc catgttgaaa caaaccagct aaaatagcaa caattgcatt ttgtccacca
     5761 cttgcaggaa gtaagctatc tgatgtagtt tcaagcccag ctttataaat taatttggca
     5821 atagcttctt tttgccattt agcaccatct ctttgtccat attggaatat ggttttaaaa
     5881 tctatttcct taattatgtg tttaaacaaa gtattcattt cttctagtgt gaaatctgga
     5941 ttcattgtgc ccatttcaat aaatgtaatt ttattattac tggacatcaa gaatagattt
     6001 gaacgtgtat cataagatac aaaagttcca cttcctgtaa ctccacttat tagacctttt
     6061 ttttcacaaa ttttaaaagc tctagagata gtactcacat taacatctaa gaaatctgcc
     6121 aattcacgtt ggggtggtaa tttagtacct ggtaataaaa atccattgtt gatatcttgt
     6181 tctagttgtt ctgcaagcgc cttatataat atttgccctt ttgaaggtct ctttggtttc
     6241 cagttcatag gataattttc aaaagaatta attggcataa gttttcacct cacattatta
     6301 atttgatttt ttctttatct aaaacaataa attctaaaga tacaaataaa aactgatagg
     6361 aaataattct atcagttttt gtcttttaat tgatttcaaa tcttcctata atttttatac
     6421 cttctttatc ataaagaggg attgtttttt taaatatatt aaaatttctt tttcttgtat
     6481 ataaaacagc ttcttctggt gtctttggtt gattatcaca atcgtacaaa tctctttttt
     6541 ttacgtagcc gactttttta cctctaccta ttgtttgtat aagttctggt tcaatggaat
     6601 ttccatttga gtctattata tgtgtaccat aggtttctcc atttttatta taattaatct
     6661 taccaatagt atattctaca tcttcatttt gtttaaaatt ggagttgata gaaaatattg
     6721 accctattat tataaaggtt aataaaaact taagacctaa attaattttc tttttatctt
     6781 gattaaacat agaatctaac ctctttttta aaataatatt ttttttattt agaaactgag
     6841 atacgaatga tgaatacttc aaagattttt gatactttat tgtatctaat attatatatg
     6901 catactcttt aatttcactc gtggtacact tgtcaatcac aatttcatca caagataatt
     6961 cacataattc atgaaaatat ttttttaata agtataaaat tggattgaac caatatatga
     7021 tacaaattat actaattaga agcttaaata agttatcttt tcttttataa tgaattaatt
     7081 catgtttaaa gatgaatctt aactctttat tattgtactt tttttctgga attattattt
     7141 tacagcgtgt taaacctata atggcaggag aataaatacc tttaacctta tatatctgaa
     7201 gttttttatt tatacttagt tcttttgctt gttcatctaa gatactagta atatctattt
     7261 ctaaaatttc ttttttaaaa ttttctatgt aattataaaa tttaaaattt gttcttaaat
     7321 agtaaaaaga gattgctata attcctaata tccagatgta cataaatata tctaaagtaa
     7381 tgccctggtc atatggtata taattatcga tattatgc
//
LOCUS       DAMJZE010000186         7218 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJZE010000186 DAMJZE010000000
VERSION     DAMJZE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.190"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..546)
                     /locus_tag="PC541_003298"
     CDS             complement(<1..546)
                     /locus_tag="PC541_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840719.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGR
                     YYVSLCCIDVDIEVFENTNNHI"
     gene            complement(1195..1602)
                     /locus_tag="PC541_003299"
     CDS             complement(1195..1602)
                     /locus_tag="PC541_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840720.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(2076..2960)
                     /locus_tag="PC541_003300"
     CDS             complement(2076..2960)
                     /locus_tag="PC541_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2840721.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(3251..3727)
                     /locus_tag="PC541_003301"
     CDS             complement(3251..3727)
                     /locus_tag="PC541_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2840722.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3993..5168
                     /locus_tag="PC541_003302"
     CDS             3993..5168
                     /locus_tag="PC541_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2840723.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(5249..6052)
                     /gene="proC"
                     /locus_tag="PC541_003303"
     CDS             complement(5249..6052)
                     /gene="proC"
                     /locus_tag="PC541_003303"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2840724.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(6092..6676)
                     /locus_tag="PC541_003304"
     CDS             complement(6092..6676)
                     /locus_tag="PC541_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840725.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2367 a         1206 c          805 g         2840 t
ORIGIN      
        1 tatatgatta ttagtatttt caaatacttc aatatctaca tcaatacagc ataatgatac
       61 ataatatcta ccacttggtt ctttagatat agtagcatta agtattctcc ctttaggtac
      121 ttgtttatcc cttatcttaa ccataccaag tttaggtagt tttatatgtt gaccacaata
      181 cataatgttt ccatttgtaa agttagtttt atatgaaaat ctattaattt tctttgattt
      241 aaatttagga aatcctgctt tttctttaaa gaatttttta tatgcattat ctaaatcttt
      301 taaggcgttt tgtagtgaaa atttgtcagg ttctttaagc cattttaatt ctttttttaa
      361 attggtcaaa tcagaactac actgtttata agtaaaagtt tctttatcgt ttttataaac
      421 atctattctt ttagcaagat acttattata tacaaatcta caacatccaa aagttttgtt
      481 aattaactct tgttgttttt tatttggata cattctaaac ttataagctt ttttcactgc
      541 tatcacctca ctttaattgt tttttgatac actttttaat gattttatca cattttatca
      601 catttaatat attatataat gctgtggtga acaatttatt aatcacctat aataatgtta
      661 acaaatgtaa ttatttaaaa caattattta gaaggtttta tatttaattt taaataacat
      721 atttaagaac cgtaagtctt aacttttatc atttttgagg ttgtcgttca cataagttcg
      781 ctactaccta tgcagttctc ttatgaactt cttacacttt catgtaagca cagactatat
      841 cttatccttc agcattatcc gttaaggtct acccacttcc actaccaata gcttgtagtg
      901 tacttccctc aagaggaata gtcgttgaag tttctcctat tcggagttta cctgctgatt
      961 gcccatttta aagtacttag gatttaacct tataccatct aaccaatttt ttctactttc
     1021 gtaactttca cacttaggtt tatttcatcc ttatgtttta gtttgattag ctttaggggt
     1081 ttccagcaat tcaagtagta ttggataggt tctatcctat ctctacatat aggtttccct
     1141 atatgctgac tattttagct actaactcat gactaaagtc acgagtgtgc gtagctattt
     1201 tttaatcaat attttagtaa attttacggt aagtaaaaac ctctctcctt caaatcctat
     1261 agcaaatata cttacaagtt gctttgagaa aataactcct aatatagcta caactatgca
     1321 tataccaact attatattta aaacattgtt taaaaacttt agtgcttgct tttcaccttg
     1381 tttactactt atatcttgat acattggaat aaaagtagta actatagcag tacctattgc
     1441 tgcaaatata atatttggta tattcatagc tgttaaataa ctctctgtat ataagcctgt
     1501 accataaatt gatgataata ctaattctct acccattcca agaatcttag agattatagt
     1561 aactatcatc aaataaaaag ttgctttagc taccttgctc attttatcac tccacttcta
     1621 acatacctct ttttaatcaa gtaaaattta gctcattaga tataattcta tcaactaatt
     1681 tgccttgctt agccctatca aaatataatt aacacctatt tcttaatata tcacaaacta
     1741 cattattctt catatttttt tatttctcca ataagtttta catttttcct atataaattg
     1801 tatagcttca attatattca aagaaatgga gttgctaccc gataacttta aaagtaaaaa
     1861 acagatttta ttatcttttt aaactatatt ctaacatatt ttgtccatat tgagaaattt
     1921 tactaaacta ttatgtactt tattaaataa ataaaataac agcacaccta taaaatacaa
     1981 gtgtgctgtt cataaacaga ttttataaaa aaataattcc taagtagaga ttaacatgct
     2041 ttctcatact ctcatatata atgtgtctaa aaatattaat aaaaatgctt taattgtttt
     2101 aacttacttg gatgtcttaa ttttcttatt gctttagctt ctatctgtct tattcgttct
     2161 ctggtaactc caaatacctt tccaatctgt tctagtgttt tagggtcatc atcatcaatt
     2221 ccaaatctca ttcttaaaac ttgctgttct tgctctccaa gacctgtaag tagttcttct
     2281 atcttttctt ttaaattatg ctgttcaact tcgtgtatta caacatcttt aaaatcagca
     2341 tctgatggta taaattcaac taaactacta tcctcatctt cttttaatgg agtatccaaa
     2401 gatacaacat ttttatctct tcttaataat tccaacactt tgtcaacttc taagccacat
     2461 gcatctgcaa tctcatctat agttggctct cttaataata catttaaaag ttcttgtttc
     2521 tttttcttta caaaatttat tctctgatga agatgtatag gtatcctaat agtattttcg
     2581 caatcgtcga tatatcttgt tatactttgt tttatccacc aagttgcata agtgctaaat
     2641 ttgtatcctt ttttataatc gtacttttct acagctttta taagtcctat atttcctgct
     2701 tgaattaaat ccaacatatc aatactatct cttttatatc tctttgctat gctcactacc
     2761 aacctataat ttgaatttat aaatttttct tttgctactg aagatgagtt gattatttcc
     2821 ttagccagct ctacctcttc tcctgctgac aacattttgt attcctctat ttcctttaaa
     2881 tacattttca tcgcattcga tacatttgat gaacccacac aaatataatt ttcataatta
     2941 ttttcttttt tagtatccat aatcaaaccc cttctattca tagaatttgc taaaacaaaa
     3001 ataaataatt taaacctaca taatctacaa ttacataata tcccattctt taaatgaatt
     3061 gttttgtata ttatgtatac aaatttataa aaactatacc tataatatta ttctacttct
     3121 aacagcaata tcctccttat atatataatt tattttatta tatgttgtta tattcttagt
     3181 tttttgaatg atatatgtct aaaaaaagac tgtttactaa acagtctttt tttatatata
     3241 tgctcaatat ttaattttca tgatgatgat tacatccaca tccacaattt tcatcatggt
     3301 gatgatgttc ttctaaatct tttacctgtt gttccatttc atgaaactct tttaccatta
     3361 tatcataagt tggaagagct gcctcagtat cgtattcttc ccctctccaa tcagcgtaca
     3421 tcatagcatc tccattagtt aaaaccaatc tacctgacat agttatatta tccacttctt
     3481 ctaaaaattc tatttgtttt tgtaaaactt ctccttctaa ctccacatct tcatctaaac
     3541 attgcactat cattacaggt gcataataat cttcttcatc tttaacattt acaacaatat
     3601 ctaaaattcc ttctgcacct tcttcaaatt ctgccaactc aacatttgta tcaatcatat
     3661 cttgaggtac taataaatct tctattaaat attttattac tctatctttt actaattctt
     3721 ttgacattta tttttctcct tccgattcta taacattatc ataatcattt ctatattata
     3781 gtatactaca ttaattgttt tttcaatctc ttacaaaaat tattttttat tatcttcctt
     3841 atattccctt ttttattaca aagttgtaat cgcattgatt taaaaaatag tttatattta
     3901 gtataataga aatgtagcac atatttttat tcaatatatt aataaaaaaa tatattgaaa
     3961 atgtaatgta tgttaaatct aggaggaata ttatgaagtt taaaaaacaa attagttcta
     4021 ttgttgtgtc tactatgctt gtacttggga gtatgaacct ttcttatgca gacggtacaa
     4081 atgtagttac acttggagct aacttatcag agtctcaaaa gcagcaaatg cttaattact
     4141 ttggagtaaa aaaagaccaa gtcttagttt tagatgtaac aaatgcagaa gaaagacagt
     4201 atttacaagg tgttgcaaca gagggtcaac ttggaaaagt tactatctct tgctcatacg
     4261 ttgaacctac taaaaaagga aatggaatta atgttaaaac agcaaactta acttgggtaa
     4321 ctagttcaat gattgcttca acccttacaa cagctggtct tgaagatgca aatgttattg
     4381 ctgctgctcc atatcctgta agtggtactg gagcattgac aggaatcatg aaagcatttg
     4441 aggatgcttc aggaaagaaa cttgatgaaa ctaagaaaga aattgcttct gaagaattgg
     4501 tagttacagg tgacttagga gatgatatag ggcaagaaaa agcaacaggt gtaatgaatg
     4561 atataaaaac agaaattgta aagaatggta caaaagatac caatcaaata gcaaatacaa
     4621 taaataatgt tgtaaataat tacaatatta ctattacacc agaacaaaaa gaacaaatta
     4681 aaggtgtaat gaaaaaaata gctgaccaaa attacgatta caatagcatg aaaaatacac
     4741 tagataatgt atcagataat gtaaatgaac aattaaagaa attaggtgaa agtgttaagg
     4801 gttctggtat actagatact attggtggat ggtttagtgg aataggagac tggttctctg
     4861 gtttattctc aggcggagat aaaaaagatt tgggaatctt aaataatact aatgatgagt
     4921 ctcttggctc tgatgctgta ataaattcta cagaaggtgt tcaaataaat ccacaaaata
     4981 atactaattc taacaatgat agtgatacaa atagctcaaa aaatgatact tcaagtaata
     5041 atgaaaataa cgatagttct caaacaaatg aaaacaacac aacaaataat tcagaaaatc
     5101 aatcaaataa taatgactca gttggtttct ttgaaaaaat aaaaaattgg ctttcaggct
     5161 tattttagaa attaaacaaa taaaaagaat tgcctaaaat ggaaattttc tataaaaggc
     5221 aattctcttt atttacaata aatttttatt actcactcat ttttttagat ttttctatac
     5281 atgcacatat tgcttttatc acagaagctc taaaaccttc ttcttcaagt actttaactg
     5341 cttctatagt agttccacca ggtgaacata ccatatcttt taattcacca ggatgttttc
     5401 ctgtctcaag taccattttt gcagaaccca taacagcctg tgatgcaaac ttatatgctt
     5461 gtggtcttgg cattcctgca attactgctg catctgctat agcttctata aataaaaata
     5521 catatgctgg tgcagaacca cttgcaccaa ttacagcttc tataagatac tctggtacta
     5581 cctcagtatt tccaaaagaa ttaaatactt ctgtaactgc ttctaagtct tctttgttta
     5641 tatttttatt tatcgaaaga gaagacatgg cttcatttac aagagctgga gtatttggca
     5701 tagttctaac tattttttta tcttctccta taatagactc tatatctttt atagattttc
     5761 ctgctgctat tgtaactata attttattgt catctataaa gtcttttatc tcttccaaaa
     5821 tatcatcata tatatctggt ttaacagcta caattactat atcagaggtt ctagttactt
     5881 ccttcgagtc ttttgtagta tttattccaa aatttgcatc tattctatcg agagttcctt
     5941 gactatatgc tgatgctata accatattag gttcaactaa ttttgaattt actattcccc
     6001 ctatcatagc gcttgccata tttcctgcac ctatgaatcc aatttttttc actttagtac
     6061 ctccttattg actaatagta atcgttaaaa tttaagcttc tttaactgca tcagattttt
     6121 caaatggatt tggtatactt actaccaaaa ttttcactac cttattagta tagttgaccc
     6181 aattatgatt gttcttttca ctattatagt gtattgtgtc tcctggatac atttctattt
     6241 gttcatctcc caaaaacact gtaagagtac cctctaatac atacacaaat tcctcacctt
     6301 catgtacgta acaacaaatt tcctcactac tcttacttgg aagaatttca attaatcttg
     6361 gtagcattgt tttttccttt aaactagatg ataaatgata atgtataaat gttgagtttt
     6421 ctacatcaaa tacttctttt tcataacttc ttaatacagc tcttttatgc tcttttggct
     6481 tcataaaaaa gtaagttaat tctacatcta gtacacttgc tatttttcct aaggaatctg
     6541 ttgccacact tgttaatcct ctttcaagtt gtgataaaaa accaattgac aagtttgttt
     6601 tttcactcat atccttcagc gtcatttgct tttgagttct gagttgcttt atttttgctc
     6661 ctatgtcctt attcaaaacc atcaccccta aataatttta attttttcat aatataaata
     6721 caaaaatata aatataggta ctgaacccat ctttatttca tttaaaaaaa tttttataag
     6781 tattactata atttatcaat ctttttttgt ctacattttt acgattattt acgatttatt
     6841 tttcacatat gtatatttaa tcttgccaac taaagataca ttttttataa gcatttactc
     6901 tttatataat aaaacttaag ttgttcatgt agtcttcttc tggtttaatt attaaaattt
     6961 caaattatat tattatttct acacattatt acatccttta gtacaattat aacataattt
     7021 tacaatttaa tgatatctat tcaatgtctt tttttactct acaacatatt ttattttaga
     7081 gtttaatact atatatattt tttatataca gccttgtcat tttttagatt gtcaagagtt
     7141 attgaagtat tttatgtaga tttagatttt atttcctaat ttctcattta acaagaattg
     7201 cttatgataa aaaataat
//
LOCUS       DAMJZE010000187         7190 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJZE010000187 DAMJZE010000000
VERSION     DAMJZE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.191"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            467..976
                     /locus_tag="PC541_003305"
     CDS             467..976
                     /locus_tag="PC541_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2840726.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1004..1198
                     /gene="rpmI"
                     /locus_tag="PC541_003306"
     CDS             1004..1198
                     /gene="rpmI"
                     /locus_tag="PC541_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2840727.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1254..1610
                     /gene="rplT"
                     /locus_tag="PC541_003307"
     CDS             1254..1610
                     /gene="rplT"
                     /locus_tag="PC541_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2840728.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1920..2465
                     /locus_tag="PC541_003308"
     CDS             1920..2465
                     /locus_tag="PC541_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840729.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2900..4825
                     /locus_tag="PC541_003309"
     CDS             2900..4825
                     /locus_tag="PC541_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2840730.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5226..7130
                     /locus_tag="PC541_003310"
     CDS             5226..7130
                     /locus_tag="PC541_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2840731.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3027 a          718 c         1356 g         2089 t
ORIGIN      
        1 tagtatatta gagtacaata tagaatttgt tatgtagtat agtagtatat tagaatacaa
       61 tatagaattt gttgggtagt atagtgttat attagaatac aatataggat ttgttatgta
      121 gaatatatag agtcatgtag aatatacata tataaaagga ttttgggtat aaatttaaaa
      181 attaatagca aaatgagtag tatagacata taattagtag taggaagtaa ttaaattttt
      241 cctaaacaaa aaaaatagtt gcaaataatt tgattaagtg atatagtaaa taaggtaaga
      301 ataaaacaaa gaagaagtcc gcttctcacc ttacgccgga ggttagtaag gtaaatataa
      361 gttaaagctt aattttaatt taagtttgtt taaatttggt aacttatatt gcggatgatt
      421 acgtcatccg ctttttattt tataaaaatt ttggaggtgt cctacaatta gcaaagaatt
      481 agcaatcaat gaacaaataa aagataagga aataagagtc ctttcaccaa caggtgaaca
      541 acttggagta atgcctacta aagaagctca agcaatggct aatagcaaaa acttagattt
      601 ggttcaaatc tcgccgaatg caaatccacc agtatgtaag ataatggatt atggaaagtt
      661 tagatatgag caagcgagaa aagagaaaga agctaagaaa aaacaaaaaa ctatagttgt
      721 aaaagaagta agacttagac caggtattga acaaaatgat ttaaatacga aagctaataa
      781 tgctataaag ttccttaaaa aaggggataa agtcaaagtt gaacttagat ttagaggaag
      841 agaattagga cataaagaca taggtaaaga agttatgctt aagtttttag atataataaa
      901 agagtttgga gaaccaacaa aagcccctgc atttgagggt aataatatgg ttgtaattat
      961 agatccaaaa aaatagatga ttaactgaga ggaggaattt gtcatgccta aaatgaaaac
     1021 tcatagaggt gcagcaaaga gattaaagaa aacaggaact ggaaagttaa agagagctaa
     1081 agcttttaaa aaacatatat taactaaaaa atctgcaaaa actaaaatga acttaagaaa
     1141 atcaacttta gtaagtgatg gagatgctaa gagaatagca caattattac catactaaga
     1201 tacagattgt aaataataac tatagaagag ggatataaaa ggaggtcata acgatggcaa
     1261 gagttaaaaa agctatgaac gctcgtaaaa agcataagaa aatattaaaa cttgcaaaag
     1321 gattcagagg atctagaagc aaattatata gaccagcaaa tacattcgta atgaaagcat
     1381 taaaaaatgc ttatatagga agaaagttaa agaagagaga cttcagaaaa ctttggatac
     1441 aaagaataaa tgcagctgct agaatgaatg gaatatctta ttcaagatta atgaatggat
     1501 taaaattatc tggtgttgaa gttaacagaa aaatgttatc tgaaatggct atacaagacc
     1561 cagaaggatt tgcaaaatta gcagaagttg ctaaagcaaa attagcttag tattgaatca
     1621 tgagatgtaa agtctttgag ttatataaat aattcaagga cttttttatt ttttgaaaaa
     1681 tacttattag tgtcatgtac aaccaatttt ataatgataa ataaaataag caatacaata
     1741 gaattttatg agatttatta taagaattgt tacaattatt tatgatagct ataaaaagtc
     1801 atagtaaaaa tgatattgat gtatgttgat atatgtataa agtttccaat caatcttgaa
     1861 ttaatattat caacatgata atattatctt attaataata taaataaggg ggacataata
     1921 tgaatgaaaa actaatggat tgttttgtaa acccaattaa atgcaagtta ttgattgaaa
     1981 ttttttcaaa taatcgaact acagctaaac aattaggaga aaaatttcaa gatatatcaa
     2041 aggcaacact ttatcgttac cttaataaaa tgttgaaaga tgatattata aaagtggttg
     2101 aagaaaataa aataagggga actgttgaaa aagtatatgc tatgaagtta gatatagctg
     2161 atgatgtgaa caaaattata gatgagaact ctggagaaga atatgctcaa ctgtttactc
     2221 aatatatgat gggattttta agagaatttt atgaatacgc tgaaagaaag gaaaaggata
     2281 tcctaaaaga tggttcagga ttttctgtct gtccagtata tgctagtatt gatgagatag
     2341 ttgaagtgag taaaaaaatt gctagtatac tagaacctct aagaaataac tcacatgaag
     2401 aaggtagaag aatgcattct ataggagtga taattactcc acctaaaaag gatgaaggga
     2461 aataaatctg atttacatgg ggtagagcca atttatacag ggaatcgttt tatgatttac
     2521 atatgtatcc agagcaaagt atctctgctt ggattgtact tcattgtacc atttcatcca
     2581 atgacttgga gtggtacaat gaagtttttt aatttttcat attaatgcta aagaaatgaa
     2641 ctttagacca catatgcaat taggttgcat tatacaatta gaccacatat acaaaataaa
     2701 aatcagtatt tttatagcag ataaattgac tgtcattctt cttaaaaatc atctattagt
     2761 tattgttagc atattgaaag aaattatagt aatctattga ataaatgaat attgcctata
     2821 atcatataaa ctgctatagt ttaaaataaa gcaggacgtt aaattttata gtaatctaaa
     2881 aatttaagag ggggatttta tgaaaataaa atttgttatt atgccattgg tgatattttt
     2941 aatggtatta tttgtggggt gtcaaagtac aaaagataca gaagttaaag caaaagatac
     3001 agaaattaaa atattaggga caggtgattt acattcaatt ttgacagact ctatggtttc
     3061 gtatgtaaat gaggaaaggg aaaagaataa aaatctgctt atggtagatg cgggagattt
     3121 ctatggtaca caaagtagag aaatgtggga atggtcttct ggaaaaaagt taattaatat
     3181 taaaagaaat ggtagagcag agtatgatga cataattaag agtagtaaag atgaagtacc
     3241 tatagttaaa gatatggcaa agttgaaata tgatgctgta gttttgggag ataatgaatt
     3301 tgtttcaaat gataagcaaa gtcttgataa actggtttct gattttaaaa ataataatat
     3361 gccattggta tcagcaaata tatatgaaca atctggagaa aattatgtac aaccatatgt
     3421 aatgaaaaat attaagacag atgagggcaa tgtaaaagtt ggaatactag gtcttactat
     3481 aaaagaagta ggggaaaccc ttggtcttga ggattttgaa aaggataaaa aagctagaga
     3541 gcttggagaa caagctgatt acaaaggaaa attatatgca aatgatttag tagaagatgc
     3601 aaaaaaatgg ataaaagtta tggaaaaaga aagtccagac ataatagttg cagttgtaca
     3661 ctcaggggaa gaacctaaaa cagatagaaa tccaggaaat agaataaaag agttagcaac
     3721 tacagtggat ggaatagatg caattgtagc tggtcatact catgaaaaaa tagatgaaca
     3781 caaatataaa aataaatctg gagatgaagt aattgttaca caaccaggtt ggcatggaga
     3841 tagagtatca gagattaatt tcaaactaaa taaagagaat ggaaagtggg acataaagga
     3901 aaagtctagt aaattaaaaa taattgataa agaagtaagt atagttgcta ctgcagattt
     3961 acattcacat ttctctgata aattaacagt tgaccttgtt aatgaacgaa gtaacccaat
     4021 tgaacctgtt gtagtagatg caggcgactt cttagaccct caaacagatg aaatgactca
     4081 atggtataaa gagtggaaaa gtataagaga taataatagt gataaaacga ttagtagatg
     4141 cccaatggtc atggatatga atcagggaat gtatgatgca gtagtacttg gaaatcatga
     4201 atttgtatct gaaaaagatg aattttggtc agatgaacgc cttttagatg ctgttataga
     4261 ggattttgaa caggtggcaa tttctgtgtt atcggcaaat atatataggc aatctggtaa
     4321 aaattatgtt aaaccatata ttataaaaga tgtggagacg agtgaaggta atgtaaaagt
     4381 aggaatattg ggacttacta taaaagaagt aggtaaaggt cttaaaaatg taaacttaca
     4441 agagcaattt caatataagg ataaattgta tgcaaatgat ttagtggaag atgcaaagaa
     4501 atgggtaaaa gttatgaaaa agaaaaaacc agatataata gttgcagttg tacattcagg
     4561 agaagaacca aaaaatccaa aacatccagg aaatagaata aaagaactag caactacagt
     4621 agatggtatt gatgcaattg ttgctagtca tactcatgaa aaaatagatg agcatgaata
     4681 taaaaataag tctggagaaa aagttatagt gacacaacca ggtgagcatg gtaaatatta
     4741 ttctaaaatt acctttaaag taaataaaga aaagggttcg tggactatta atgataaatt
     4801 cagtaaaaca attgaggttg aataacttta taaaatttat agataaagta aattatctga
     4861 tattggcatg atatataaga taaagtttct aatattagta taatgcataa tgtgaaatat
     4921 tttaataata agagaataaa caagacaaat aaaaatattt taacatgtaa gatatagtat
     4981 gaactattat actaatgctt actttataat ttgaacatgt tagaataagt cttatttgaa
     5041 aattttagtg tactagagta agtattattt tactagtaaa aaatattgag aactatatgt
     5101 agatttgctt aaaaaggaat ttacatatag ttcttttcgt ataataaata gtacaatctt
     5161 atatggtaaa gataaagtgt gatattaaat tttattatca tccaaaatct aaggaggagt
     5221 ctcttatgaa aataaaattt attatagtag catcaataat atttttaata gtattacttg
     5281 tggggtgtca aagtactaaa gacacagaaa tcaatgtact cgccacaagt gatttacatt
     5341 caattattcc agataattta atttcctatg tagaagagga gagaaaatat gatgagaatc
     5401 tacttatggt agatgcagga gatttttttg acatgcaaag tggtgagatg aataagtggt
     5461 ttactggtca aaaattagta agatttaaag atgggatacc agagtacaaa aaaatagctg
     5521 aaccacgtga aggagaagtt ccattggcta aaaaaatggc aaagctgaag tatgatgcag
     5581 ttacatttgg gaatcatgaa tttgtatcta atgacaagca gagccttgat aaattggttt
     5641 ctgattttaa aaataacaat ataccattag tatcagcaaa cgtatatgaa caatctggag
     5701 aaaactacgt aaagccatat gtaatgaaaa atataaatac tgacaaggga actgtaaagg
     5761 ttggtatact tggtctgact ataaaagaag taggggaacg ttcaaggttt aaaagttttg
     5821 aacaagataa aaaggctaga gaacttgggg aacaacctca atacaaaggg aaattatatg
     5881 caaatgattt agtagaagat gcaaataagt gggtaaatgt tatggaaaaa aaagaaaagc
     5941 cagatataat agttgccatt gtacattcag gtgaagagcc aaaaaatcct aaaaatccag
     6001 gaaatagaat aaaagaacta gcaacaactg tagagggaat agatgctatt gtagcaggtc
     6061 atacacatga aaaaatagaa cagcatacat ataaaaataa ttctggtgaa gaagtaatag
     6121 taacacagcc tggagctcat ggtaatagta tatcaaagat taattttaag ttggataaga
     6181 agaatgataa gtggtttata aaagacaaat atagtgaatt aaaggttttt ggaaaggaag
     6241 taaatataat tacaacttct ggtctgaatc caaaattttc agatgaaatg gcagttaatt
     6301 tgtttaatga aagagataga gatgttcaac ctatctttat agatactgga gattttttaa
     6361 atactaatac aaaagaaatg actaagtggg ctaaagagtg gcaatacaat gaaaataaag
     6421 gcatctataa aaaaataaat gaatggccaa ttgtatatct tggttatgat gctgtagtac
     6481 ttggtagcca tgaatttaca gaggataaag agacttttga ggaaaaaaaa tctacattgg
     6541 attatattat agaaaacttt gaagaaatga agataccagt attatcagct aatatatatg
     6601 atgaatctgg agaaaattat gtaaaacctt atataattca taatgtagaa actagggaag
     6661 gaaatataaa ggttggagta ttaggtctta ctactactga taaggatgaa gaagacttaa
     6721 gtaactcaag cttaagcaaa gactcaaaga cttcgaaaat tcctgaagaa acaggaaagt
     6781 tatatactaa taatctagta aaagatgcaa gcgaatgggt aaaagttatg caaaaggaaa
     6841 gtcctgatgt gattgttgca gttgtacatt cagataataa tagtactcta aaaaatccaa
     6901 gtagtgaagt caaaaaactt gcaatgtcag tagatggaat agatgctatt gttgtaggtc
     6961 atacgaaaaa taaggtagaa gaacatattt acaaaaataa gtctggggat gaggttatag
     7021 taacacaatc tggagaagat tgttattcaa aaataagtct tgaactaaga aaagaaaatg
     7081 gcaagtggaa tatcataaac aaatatagta aagcaataaa aatgaagtga atttattgat
     7141 atataaacca ttattaacac tgatttttat aatataataa atactgattc
//
LOCUS       DAMJZE010000188         7108 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJZE010000188 DAMJZE010000000
VERSION     DAMJZE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.192"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(197..1036)
                     /locus_tag="PC541_003311"
     CDS             complement(197..1036)
                     /locus_tag="PC541_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2840732.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1060..1818)
                     /locus_tag="PC541_003312"
     CDS             complement(1060..1818)
                     /locus_tag="PC541_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2840733.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1865..2338)
                     /locus_tag="PC541_003313"
     CDS             complement(1865..2338)
                     /locus_tag="PC541_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2840734.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2367..2783)
                     /locus_tag="PC541_003314"
     CDS             complement(2367..2783)
                     /locus_tag="PC541_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2840735.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2830..3882)
                     /locus_tag="PC541_003315"
     CDS             complement(2830..3882)
                     /locus_tag="PC541_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2840736.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4278..4958
                     /locus_tag="PC541_003316"
     CDS             4278..4958
                     /locus_tag="PC541_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2840737.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5091..5411)
                     /gene="sugE"
                     /locus_tag="PC541_003317"
     CDS             complement(5091..5411)
                     /gene="sugE"
                     /locus_tag="PC541_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2840738.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5793..6620
                     /locus_tag="PC541_003318"
     CDS             5793..6620
                     /locus_tag="PC541_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2840739.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2406 a         1296 c          839 g         2567 t
ORIGIN      
        1 aagcacctca ttctgattaa aaatctttct gagatacttt ttaaaatatt aaatactaca
       61 tatagcttac ataagtttaa aatttaatct attataaaat taccctaatt gccaaacttt
      121 ttaatataaa ttttccaaca tcctacaaat ataatcaata gatatagaat cctaacttac
      181 accaatatat atttatttaa aatagtccta aaatacctgt aatagctccc actactatca
      241 gtataaacat tatttttatt ggagaaagac ctttttttaa tgcaaagagt actaataaag
      301 taagtaccaa aggaattaat cctggaaata tcttatcaag taaatccgtt tgaagactaa
      361 atttaaaatc accactagta taacttattg gtgtagataa attgataaac ctacctacaa
      421 gcccccccat gactaaggta cctagtatag aagccccttc tataacctta tttactattc
      481 caccttgaag tatactttca acagcttttt tcccatactt atatccattt gtccacattc
      541 caaaagaaat tgaagtcatt attatatatt gagctattac aaataatata ggtcctgcaa
      601 ttgatgctcc tccctcagtt aaaccaatac aaacagctaa cactatagga gttataactc
      661 cttgagttaa agtatctcca attcccgcta gtggacccat caagcctgtt ttgatagcat
      721 tcataccatc tccacttatt tcatctgctc cattagcttt ttcttcttcc atagctattg
      781 ttattccatg tataacacat ccacatatag gttctgtgtt aaaaaatgct agatgagtct
      841 ccacttcttg ttttaactct tccttatctg gatataattt tttaagtact ggcaacattg
      901 aatgtgaaaa agctgttgat tgcaatcttt cataattgta attagattgt gcaaagaaaa
      961 accatcttaa ccatgatttt attacatctt tcttgcttaa cgttttctta gatacttcat
     1021 tttttatatc tgacataata ttttccccct cacatgtatt taagatgtag cactatcttt
     1081 tccgtactta attgaagtaa ttgtaagagc aagtattact ccaataacag atattactat
     1141 tatatctact ttaaaataaa cagccattag tattcctaat atgaaaaagg gcatagtaaa
     1201 cttatttcca atcaatttta aattcatagc aatcccaagt gctggcaaca tagccccaat
     1261 tacattaaat acatgtaaca cattatcatt cattacttcc aacataccat ctatcgcatt
     1321 tggaccaaaa taaacaacta ataatgctgg tataaaactc attgcaaata acagtatctg
     1381 tggtggtact atatttataa atgctactct atcaacattc cctttttgaa cctctctatc
     1441 agcccaatgt gcaaaaaaag aatttactgt catctttcca atccaaagta cagttgcaac
     1501 agttcccaat ggtacagcta ttgccattgc agtacttaca tctagcttag atgctatagc
     1561 taaagcagta cctacatatc ctgcaaaagc tgggtccccc atctgagctc cccctgctga
     1621 tataaaacct agatatatca tatttatacc tgctcctata attgctcctt gaacagggtc
     1681 tcctaatata attcccgtta caaatccagc caatagaggt ctattccatg taaaaaatcc
     1741 aacataagtc aaccacggac tccaagaaag ataataaaaa attgctatta atactgcttg
     1801 aattagtgaa atgtgcatat tcttcctccc tattttcttt taaagattta caagatagtc
     1861 ttctttacaa taatgttcct acatcaattt tctttgcatc tggtactatt tgtataaagc
     1921 atttaacatc ttttgatatt agctctctaa atatttcttt ttcttcatct gacacagata
     1981 tatttttata aaaacttttt ctaccaagcc ctgctcccat acctccaaca ttcaattcct
     2041 ttaaatctac cccactattt actaaaccta agacagtttt agggtatttg accaatacta
     2101 ttaaattttc atctccatta tgtgatttta agacttcagc acctgattct acatcatgta
     2161 cttcaacctt aacagttgat ggagctgcca tttgtaaaac ttgaactata aatgggtcct
     2221 ttactactaa atcatccacg attactatcc tttttgctga tgtaatctta caccaagcag
     2281 taataacttg cccatgtatt aatctatcat caattcttgt taatgctata ttactcataa
     2341 ttattcctcc agatttttaa tttattttac aactttaaaa cagttttaac atctttgatt
     2401 cctgatatcc caacttcctc acataaattc ttcaagtctt ttaacttcat agtatctctg
     2461 tttaaaaata actctaaaag cataggtaaa tttacacctg tcaaacattc taactcacat
     2521 accttagcaa atttatttat tacaatagca gatgtattaa atggagttcc gccataaaaa
     2581 tcaagcaata ctaaaacccc ttcatcctta tatttctcaa ttccatcttc taactttcta
     2641 ctaaactcat ctatgctgtc accatggttt aatcctaaag tttctacatt ttcctgtttg
     2701 cctactatta attcagcact ttctaacatt gctttagcca tatttccatg tgtggctata
     2761 attattcctg ccatatgaat catacctttc ttaacctttt ttatatggat tattatttag
     2821 tactctaatc taattatcta tattaatcat tatcttacca aagaaaatat cccttttgta
     2881 tactttttca aaataagctg gtacttcttc taaatttatt ctatgtgtta ctaatttttc
     2941 aacattcata tcaccctttt gcatatgata caatccagca ctccattctt ttcctgggaa
     3001 tggaaaggag tttccaaacc aagttccctt tacagttaat tcacttctta ctatcttttc
     3061 aaattgctct ctagtcaaag ctacatcccc atatggtaca cctgcataca atactgtacc
     3121 tcccttttta gctagcaaca gtacttgacc acatgtcagt ggagtaccag ccgattcaat
     3181 aacaatatct gcaccatctc catcagttaa cctttttatt tcttctacaa tattcttttc
     3241 ttttgcattt atacaaatat ctgcccctaa ctcttttgcc aagtctaact tctcatcaaa
     3301 tacatccaca gctattattt ttgtagaacc aaatattttt gcccattgta ttgaaaataa
     3361 tcctattgga cctgtaccaa gtactacaac agtatctcca actttagctt ctgacctaaa
     3421 aagaccatgt cctgctatac atactggttc tagcgcagcg gcagtctcat aacttatctc
     3481 atctggtatt tttattaaat ttctctcttt cacctttgta tactctgcaa aacaacctcc
     3541 aagttcttta tttcctatta ttgctacatt attacatctt gaatacaagc cttttttaca
     3601 ttcatcacat tcaaaacatg gcatagctgg acaaacagca actctatctc ctattttaaa
     3661 actccttact tctttaccaa cctgtgccac ttctccagaa aattcatgcc ctaaaatttc
     3721 tccaaccata tgtggacctg tctttgaata ttttgatata tcagaaccac atattccagc
     3781 tacttttact ttaattatta catcatcttt ttctaatatt tttggtacat ccacatcctc
     3841 cactcttgtg tctcttatac catagaatct aactgacttc actattaacc acccctactt
     3901 tttatttgag aatatatccc tattgtttat atgttaaata tctaaagcaa ctttcatgcc
     3961 aatatatgta agcattgtat tcatgcattt tataaaatta tcattaaatg tgtgtcaaag
     4021 tgtgtcaaaa gtttcttata ttaaaactta tgtgtgtcaa tataaacgac actaacattt
     4081 tatattagtt ctcttattat aagttttata aaatgtactt tttttgttat ttttataaaa
     4141 ttgttcggaa atttcccttc tatttgtgat ataatattaa atataaggta tcaatataaa
     4201 ttttcatgcg aatatcagaa cttttttgac actaatgcct tattcaatat aataatttaa
     4261 ctttttggag gttgtttatg aaaaaagtgt atcaaggaaa gactaaagat gtttttgaat
     4321 tagataatgg aaattactta cttaaattta aagacgatgt aactggtaaa gatggagtat
     4381 ttgacccagg cgaaaatagt gtaggtctaa gtatagatgg aattggtaga gctaatttag
     4441 agacatctgt taagttcttt gaaattctaa acaatgctgg aattaaaact cattatgtaa
     4501 gtgctaattt agaagaagca actatggaag ttctaccagc aaaagttttt ggtaatggat
     4561 tagaagttat ttgtcgttat agagctgtag gtagtttctt acgtcgttat ggttcatgtg
     4621 ttgaagaagg tgctaaacta gattgttacg tagaagcaac tttaaaagat gatgaccgtt
     4681 gtgaccctct tataacatct gaaggtttag aagctttaaa cattatgact caagctcaat
     4741 ttgattcaat gaaaagcatg actcaaaaaa tttctaacat agttcgtgat actatagctg
     4801 aaaaaggtct tgagctttat gatattaaat ttgaatttgg attctacaat gatgaagtaa
     4861 tcttaataga tgaaatagca tctggaaata tgcgtgttta taaagatggt gtaatagttg
     4921 accctatgga tttaactgct cttttattag ataaatagta tatacatata atttacaaat
     4981 ataataaact tataaataag ataagctatg aagtaaagac tttttaaatg ttgattattt
     5041 ttaaacattt tctcgccttt aatttcatag cttatttaaa ttaatatact ctaattagta
     5101 gataatagct ttaaccctac aattcccacc acaataaaac ctatacatgc aattcttgat
     5161 atactaatag gctcatttaa aatagccatg cctacaatag ctgtaccgac ggtaccaatt
     5221 ccagtccata ttgcatatga agttccaagt ggaagatgtt ttagtgctaa tgacaaaaaa
     5281 taaaaactta caatcattcc aacaacagta agtatacttg gagttaattt agaaaatcca
     5341 tgtgaatttt ttagttgaaa agcccaaaac acttcaaaca aacctgcaac aaacaaaaat
     5401 aaccatttca taaattcatc cctcttttca aatatgtctc ttatttttcc aataaaataa
     5461 cctgagaaat attataaaat atctcccagg tttttgtcct tccgtgttca caacaaaaac
     5521 tatgttgtgg gttttatctc ggaccagacc aatcattaat aattgcggaa ccctataaaa
     5581 ctctattaat tattctattt tatctataat aatactatca acatatataa aaaaagtcaa
     5641 tatatgggtt ccatatataa taaaaataac ttttatatac cttttaatat tcttattcac
     5701 ttctctccat acccatttgg gtcaatctaa gaatatctaa tttttattta atatgttata
     5761 ataacataaa aatattgaaa ggagtaattt ctatgaattt ctataaaatt gcagtatgtc
     5821 agatgattac tacagaaaat aaaattgaaa atatcaatca tgctgtagat atggtaacag
     5881 aagctgccat caatggagct aaaattgtag ttttgccaga aatgtttaac tgcccatatg
     5941 aaaataaata ttttcctaaa tttgctgaag aatatccagg tgaaacaaca acgatattaa
     6001 gtaaattagc tgagaaacat ggaatttatc tagtttctgg tagtattcct gagttagaag
     6061 atggaaaaat ctataatact tgctatgtat ttgataaaaa tggagctcta attggaaaac
     6121 atcgtaaaat gcacttattt gacattgaag ttacaggaaa agttagtttt aaagaatcag
     6181 atactcttac agctggaaat gatgtcactg taatagatac cgaatatgga aaagttggta
     6241 tagctatttg ctatgatatt cgtttccctg agttatcacg tttaatggct cttaaaggtg
     6301 ctgaaatcgt tatcttacct gctgcattta atatgactac tggtcccgcc cattgggagt
     6361 tatctataag aatgcgtgct ttagataatc agatattcta tgttggtgct gcacctgcta
     6421 gaaatatgaa tgcttcttat atagcttttg gaaattcaag aatttcagac ccttggggaa
     6481 gaataattgc tcaagctgat gaaaaagaat gtattattta tgctgacatt gatagagatt
     6541 taattcctga cataagacaa cagttaccac ttcttaagca cagaagaact gatttatatg
     6601 aacttaatac tttgaaataa aacagaacat aaccccataa atcataaatt aagtgttaaa
     6661 aaaatttatg gggttctcat taatttgatt taaaattaat ttcataaaaa taatgctaat
     6721 attaatttaa ttaaataata ctaataatta ctaatgatta tgctaattat cagcatttac
     6781 taatttaatc actattagtt atttctttta ttaaatatct ttactttttt acttattatg
     6841 tcttctagct ttacatttgg attatggtat ttctttctat ttttagtcac atctttctat
     6901 tgaattactt gcttaaaaca ccaatgcaca catgccagat aatgttcaca attatttaga
     6961 aatcttgctc taccttcttc aatgttgata ttatctctcc tagttgatat tatctttcct
     7021 aaaatttctt ttaattaaaa aacaacatta gttgatatat caagtatatt ttaagcaact
     7081 gaaaaaaaca ataataattg atatatca
//
LOCUS       DAMJZE010000189         6960 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJZE010000189 DAMJZE010000000
VERSION     DAMJZE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.193"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            87..1490
                     /locus_tag="PC541_003319"
     CDS             87..1490
                     /locus_tag="PC541_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2840740.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1709..4186
                     /locus_tag="PC541_003320"
     CDS             1709..4186
                     /locus_tag="PC541_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2840741.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4201..4887
                     /locus_tag="PC541_003321"
     CDS             4201..4887
                     /locus_tag="PC541_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840742.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5161..5994
                     /locus_tag="PC541_003322"
     CDS             5161..5994
                     /locus_tag="PC541_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840743.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            6032..6493
                     /locus_tag="PC541_003323"
     CDS             6032..6493
                     /locus_tag="PC541_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2840744.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            6524..6772
                     /locus_tag="PC541_003324"
     CDS             6524..6772
                     /locus_tag="PC541_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2840745.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2684 a          749 c         1080 g         2447 t
ORIGIN      
        1 taaattttta catatcaata attattgtct attttatata agcaacaatc aaactaaaat
       61 aaaagtttaa gaaaagagtt gattaaatgc ataaacctag ttataaaaca gtatttcatt
      121 catacattat ttttttcatg atttttattt gcttaatcct gacagtaata ggtattttct
      181 tatcacttat tactattcag aaaccaaatg gggaatttat tcgtagtgac tggccgaaaa
      241 aatttacaga aaactttaat gaacaaatcg tatttataga taataagcca caagtaaaac
      301 agtctggatt agaaaaactt cgagaaaatc aattgtggtt gcaaatttta gattctagtg
      361 gaaatagagt ttatgggttt ttagaaccaa aagagcataa agattattat tctaattctg
      421 aattattagc attagctaac caaaataatt caagtaatgg aactgtatac tttaatacaa
      481 ttaaaaataa taaaaatgac tatatttata ttatatattt tccattaaat atatctaaag
      541 tgaatatgtt ttttgatgga ggtaaattta taggtggtaa gtcaattgta ttttcaatca
      601 tagggattat atttttaata attttggttt ctggaattgc ttatggttac tgggtaacaa
      661 agtgcatatc aaatattaca tctgctgtta gtgatatttc aaaacgaagc tatcttccag
      721 ttaatattaa aggtgttttt gctgatatat ataatagttt aaatatttta aatgaagaaa
      781 ttcataaaag tgatgagata caaaataaaa ctgatattat gagagaagag tggatttcaa
      841 atataactca tgatttaaaa actccacttt caccaataaa agggtatgca gagcttctta
      901 cagatagtag tactaattat tcacctgaaa aagtatataa atatgcttca atcatgttaa
      961 agaacataga atatacaaat acacttataa acgatttaaa attaacctat caattaaaaa
     1021 atggaataat ccctttaaat aaaaataatt atgatttagt aagattcttg aaagaattat
     1081 ccattgatat tttaaattat ccagaatatg aatcaaatat agttgaattt aagagtaata
     1141 tagataatat taactttgat tttgattgta cactattaaa acgagctttt agtaatctta
     1201 ttgtcaatgc ttttacatat ggagataaaa atactaaagt ttgtgtacat gtaacattaa
     1261 atgaaagaat tgaaatcatt atatctgata atggaaaagg gatgtcaaga gaagaagcat
     1321 caaatttgtt taatcgttat tatagaggaa gcaatacaaa acaaaaaata gaaggaactg
     1381 gtttaggact agcaataacg aagcaaatta ttgaagttca tggagggaat atatctgttg
     1441 aaagtatacc tggattaggt agctcatttt atatcacctt tcctatttaa ttaaggttaa
     1501 ttaaggttaa ataaaaaata tattaaggtt gatagattat tatatttata tagtagttta
     1561 tcaatctttt ttatttaagg ggtgatttga ttaaaaatat tataaataga atgataaaag
     1621 tatggaggag tctattagag atttaattta gattaaagta taaaaatatt agattgaaat
     1681 atcttagata agggaagaag gtgtaagttt gaaaattata ttaaagtata ttttcacaaa
     1741 tattaaagaa cgaaaaatta gaacagcagt tatgttattg tccatagttt tatcaacagt
     1801 tttacttttt gtttcattct caattggatt atcttatgaa agtgcacaaa gaaaaatggc
     1861 aaaaggtatg tatggtacag cgactatatc tgtccaaagt aaaaatccag atatattaac
     1921 caatttagaa gacatacctg atttaaatac tataaaatca aaggttggtg tattggaaag
     1981 ctctgctatt tataataaag ggggatacta tgaagaattt agtcttatat cagcagactt
     2041 aagtcaacta aataaaatca acaagccacg attagaaaat ggcgatagta ttactgattt
     2101 ttctggtgat aaaattattc ttccaaatcg ctttacatca aaatataaaa taaaaaaagg
     2161 tgattctatt actttgcaga tttatggaaa gtcttatact tttcaagtta gtgatattgc
     2221 atcttatgat acagtatttt tgaggaacac aagaggtgta aatgcactgc tacccaaaga
     2281 gactctatca aaaattataa ataaaggcag tggatataca agagttctta tagagtcaga
     2341 agaagaaatt acagaaaatc tagtaaataa attatccgaa gaactttcta ctgaaaaata
     2401 tatggtatca aatactataa atgaaactaa gataatttca gatgctagac aaaagacaat
     2461 gcctttcttt ttaataagtt tttttgcatt aacccttagt atatttataa tatacagcag
     2521 ttataaggtt attacattag aacgtcttcc ttttattgga acatttagaa gtattggtgc
     2581 caatgaaaaa actgtaactc gtatattgat gcttgaaagt atattatacg gaagtatagg
     2641 aggattaatt gctattccta ttggtgtagt tgttttaaat ttaatgcttc atgggttagg
     2701 aaattctctc gaacaaggaa tatcaattcc agtagtaata tcacccattg gagtgattat
     2761 atctgttata gtagcaataa tcgtttcttc atttagtgca tatatacctg ttaaaaaagc
     2821 tagccatttg ccgataaaaa atattgtact aggaacagta gaagaaaaga atgtttcaaa
     2881 tcgttctata ctttttatag gaagtataat gtttatttta tctattttac ttccaagaat
     2941 atcccctgaa aatacattgt atctagctgg tggattttca ttactaggat taattgtagc
     3001 tactattgtg ttgattcctc ttattactga tatcatgtct atagtttttg agtttgttta
     3061 taaaaatatt ttagggaatg agggtaaatt agctgctaga aatatgaaaa ataacaaaaa
     3121 tataatacag aatatcactt tactatttat aagtatttct gcagttattg caataagtgt
     3181 tgttggtaac tttgtcaaaa cttatataac agatgtgttt cgtgacgcag aattacaagg
     3241 ttttgcagat ggaaatatga atgaagagtt tattgaagat gttagacata tggaaggaat
     3301 taaaaaaata ttacctcttt atgttatgaa taatgagatt tctggtaatg gtgtaacatt
     3361 atcaagacta gagggtacag ataatataaa actatataac tctatgtttg gaatcaatta
     3421 tacaaatttt gagataaaaa aacaagtaat agaagctttt aatgataaac gttctgttat
     3481 tttgaatgaa gatactctaa aaaaagtagg cttatctata ggagatacaa taactctatc
     3541 taatgataag tatgattttt cttataaaat agtaggtagt tttaaatccc gtgcaaatga
     3601 tgtagaagct gttataccat cacattatgc agttaatgat tttgataaaa ctaattatgg
     3661 ttttttagta tatacagcag tcaatccaga tgctgttatg attcagataa gatatttatt
     3721 tggagataca tataattgga gtcgtacagt tgaagaattt aataatgatt ctttaaatac
     3781 tataagtagt tttttgtctc ctatgaataa gatgacctat ttcatttttc ttcttgctac
     3841 agtaggtatt attaataatc tactgattaa ttatattcaa aaaagacgtt ctatagctat
     3901 gtataaatct ataggactaa gtaataaaca aaatataaaa gtaacattga ttgaaggggt
     3961 tacttcaggt ttacttggag ctgttattgg tattgtgata tctatacttg agattcaaac
     4021 tatctttata gttgcaggac caaaaatttc aatgaaacca gatttagatt ttaaaacatt
     4081 tattattgtt gggttattgg gaattatagt aactttaata ggttcgatag tgcctattat
     4141 aaaagggaag aaaatgaaac tgatagaaga aattaagttt gaataattat ggaggttaat
     4201 atgaaaatta cacataatag tttagctgta gaagcaaaaa atattatcaa agaatataaa
     4261 attggaaata caacaactcg agtgttaaaa gaggtatctt tacaagtaat gaaaggtgaa
     4321 tttgtttcta tcatgggtca atctggttct ggtaaaagta ctcttttata tattttaggt
     4381 ggtttagata ctccaacgag cggaaaagtg tatatgaatg gagctgatat atcacatttt
     4441 aatgatgaga aaatgagcat aatacgtcgt agaaatatag gattcgtatt tcaattttat
     4501 aacttaatac caaatttaaa tgtggaggaa aatataatgc ttcctttgtt attagatggt
     4561 aaaaatttaa aagattataa aaatcaatta gatgaaatct tagatatagt tggtttaact
     4621 gatagacgta aacatacacc acgagagtta tctggtggtc agcaacaacg tgttgctata
     4681 gctcgagctt taattggaaa accagagatt ctatttgctg atgagcctac tggaaattta
     4741 gatagtaaaa cagggataga aataatagat ttactgaata aaataaacag agacaatgga
     4801 caaactataa ttatggtaac tcactcacca gaagcagcca aaagtagtag tcgtactatt
     4861 acagtttcag atggtttaat tgtataagga ctaattattg tatttgagat aagcattttt
     4921 aattatataa attagacata tgtaaagtgg atataagtta aatactataa atgtgctata
     4981 atattaaaat aacagataaa agtactatat tttttattga ttattttaat tttatttgtt
     5041 tattaaatat ctaatgagta ttcatactat ctctattatt aaaagcagtt aatgatatat
     5101 agagataata tggattcttc ttactttaat aaatattcac gataggacag gatataatat
     5161 atgttaaaaa aagaaagtat tcattttata aataaaaata ggtcacttat agtttgtgaa
     5221 gacaattttg tttatctctt gccttgtata caattaagaa attggatatc gaattttact
     5281 atatcgtttc caaatcaaac aataatttct gataattaca cgattattcc tcatggaagt
     5341 gtaactttgg tgttttttta tgatgccaca gggttacata gtctattatt tggtccaact
     5401 actaaaccaa aaacagtagg aaatattgct aatagatgtg atgttatact aattatagaa
     5461 tttcaacctg ctggtttttt cccgcttata ggtatacagc aaagtgaact tattgataaa
     5521 gttgttccat tttctataat taatacttca ttagatttag aaattaaaaa aatatttaat
     5581 gaatctttat ctatagataa attgatttta aagttggaag aattattgat ttctaatatt
     5641 aaaattgaat actcatatga gtttattttg gcaattcagt tgattattca aaactctgga
     5701 aatatatctt cacaagaaat ttctaaaaaa gtattttata gttctaggca tttaaacaga
     5761 ctctttaatc agtatctagg gttgagtatg aagtcatttt ctcgtttggt acgtattaat
     5821 aaatctataa aacttctaaa caataataaa acttcatgga tgtctatatg taatgaatta
     5881 ggattctatg atataccaca ttttattaaa gattttaaaa ttgtctgtgg aataactcct
     5941 caaaaatacc gagctaatat gtctgatttt tacagtgaaa ttgcaaaatt ttaagttata
     6001 atacttataa caaagaaagg atagtgataa gatgaaattt ggaggagtat tgctaattgt
     6061 caaagacata aataaatcaa aaaagttttt tgaagatgtt atggagcaaa aaatcacatt
     6121 agatttagga gaacatgtat catttgaaaa tggatttgga ttacaaagta attataaagc
     6181 tataattgga tatgatttac caatatatca acaatcaaat aactttcagt tgtattttga
     6241 ggttaatgac atcaaacaat gggaaagtaa gataaataga ataggaaata ttgaattttt
     6301 gcattatata aaagaatacc catggggtca aagaactttt aggttttatg attttgataa
     6361 aaatattata gaaatttcag aaagtatgga aagcgtcata aagaggcttt taaaacaagg
     6421 tttagcttta gaagaaattt caaaacgcac tatgtatcca gttgaattta ttacacaatt
     6481 tcaacaagta taaaatatta aaatatataa ttttttcttt atattgcaaa aaacaaaggc
     6541 ttggggcata atagaaccag aaaaacctga atctcaacag caaatggctc atatggattt
     6601 cgctgttaag gatttagaag aagcagttca atatgcaatt cattgtggag ctacaatcgc
     6661 agaggagcaa tttactgatg actggagagt tatgattgac ccagctggac accctttttg
     6721 cttatgtcag atgaaatcaa ttatggaaag tagccatttt tcattgttat agaccaaaat
     6781 attgtttatt aattacaaaa aagcacaaca gtatataccg ttgtgctttt acttattata
     6841 catgaataca ctaaaaaata taaatagtag agctttatca ataatgataa tttttaaaaa
     6901 atagtatttt tttattgaat aatttcttag atattttaaa gtatataaat tttgcatata
//
LOCUS       DAMJZE010000190         6891 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJZE010000190 DAMJZE010000000
VERSION     DAMJZE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.194"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(331..1227)
                     /locus_tag="PC541_003325"
     CDS             complement(331..1227)
                     /locus_tag="PC541_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2840746.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1368..2795
                     /locus_tag="PC541_003326"
     CDS             1368..2795
                     /locus_tag="PC541_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2840747.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2991..5585)
                     /gene="clpB"
                     /locus_tag="PC541_003327"
     CDS             complement(2991..5585)
                     /gene="clpB"
                     /locus_tag="PC541_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2840748.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5622..6218)
                     /locus_tag="PC541_003328"
     CDS             complement(5622..6218)
                     /locus_tag="PC541_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840749.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6235..6639)
                     /locus_tag="PC541_003329"
     CDS             complement(6235..6639)
                     /locus_tag="PC541_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2840750.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2218 a         1103 c          715 g         2855 t
ORIGIN      
        1 tttaaatttt ctataaaaat catactattt tagctgttta cttgcttatt taatagtttt
       61 atttctttga ttaaatatct ttcagaatct aagccagatg tctcaaaaaa actgatttat
      121 aatataatca ttttttctgc tattcttatt tttccaattt tagtttcata ttaaaatata
      181 cttttcatag acactacatt ctttcatact cattttcaca tttaactata caagtagtct
      241 tactcattta tgtactatac tttattaata tttattaaga aaaactcaaa gatttaaaaa
      301 tatatatttt ctataaagta tatttatgaa ttatttcata tcttctttta aaataacatt
      361 actaacttct ttatgttcat ttctatactt agttggagat atattataat tccttttaaa
      421 taagcgacta aaataattta tgctatcaaa gccacatctc atagcaattt ctgtcatatt
      481 taaatctgtt tcctttaaat aatatatagc tttatcaagc ctgattccat ttataaaatc
      541 gattgtagtc ttacctgtta tttgcttaaa tgttctacaa aagtgatgtg cactcaagtt
      601 cacatgacta gcaagttctt ttaatgtaat tttatccata tatttttctt ctatcaaatc
      661 aaagatacat tcaaattttt ctaaagaata ctttcttttt ataaattcat cttcagaata
      721 tattttatca atatgacttc ttaataagag tactattaat tgatagatat tagcttttat
      781 agataactca tatccaactt ctttattgtg atattcatca agtatagttg taatacattt
      841 taaaatcaga gaatcatttt gaattaaatt attaaataca atatgattta aagccaatgg
      901 tgctaagtac ttggtttgta acaaatcaac ttgattacta aaaaggaatg tgggttcaat
      961 acgaatacta tagaatttta aattatctga taagctttct aaataatgta attcattact
     1021 gtttgctatt acaatatttc caggcactac cttaaaatta tccaatccac attcaagata
     1081 tgctatacca tcaataaaat aatatatttg tagatgctca tgccaatgtc ttttaaagtt
     1141 tgctcctttt ttaatgtcat cctttaaaaa aatatctata aagttttctt gacctttcct
     1201 gttaaaatat gtatttttat ccttcattat tcccccctca tcaaaatagt gctaattata
     1261 agcaatatag ttattgtatt cattatttca tatcgctatt atataaaatg taactatcat
     1321 tgtaaacatt aacataatgc agtcattata ataaggaggt aataaaaatg acaaactctt
     1381 ttaaaaaaga tgataataga aaaaaaatca cagttttatt tgtagtcatt gccatgactt
     1441 ttatggcaac attagattct agtattataa atgttgctct cccagttctt gcaagcaaac
     1501 taaatgtatc tctagcatct attgaatggg taatagcaag ttactcaatt attatttgtt
     1561 caacactatt attttttgga agacttggag atattattgg taaatcaaga gtatttcaag
     1621 taggtactat tctctttaca tcagcttcat tactatgtgg attaagtaat tctttaactt
     1681 tacttatagt ttgccgcttt attcaaggaa taggcgcttc tgcctacatg gcaaacaatc
     1741 atggaattat cacagaacta tttccaaaag aaagcagagg aaaagcccta gggattttag
     1801 ttactgcagt tgctattgga aatatggttg gaccatctgt gggaggattt attttgtcaa
     1861 tatttgattg gaatgttata ttttttataa atattccaat cggtcttatt gtaatctttt
     1921 taaatacaaa atttcttcct aatagtaaaa aatcttcaga aaatatggat aaaactggtg
     1981 ctatacttca atttttagga acaacattat tttttagtgc tctaattagt gctcaacaaa
     2041 caggattact aaatccatat atattaattg cactattatt atcaattatt tttattatat
     2101 tatttttgat attagaaaaa aaacatcctc aaccgcttct tgaccttgaa atttttagaa
     2161 attttaaatt ttcattgaat ttaatttgtg ctcttacatc attcatttgt attgcatcat
     2221 caagtatttt aattccattt tatttacaaa gtactatgaa acttccccct attcaagcag
     2281 gtttatttat gatactatca cctttgattt tagccatttt ttcacctata tttgggaata
     2341 tatcagataa aattaagtca gaaaaaatta ttttaattgg tcttctcgtt atgagttttg
     2401 gattctttct aatgtcaaga ttgaaagaaa gttctgccct tattttattt gtaatttata
     2461 tatcgataat atcaatagga caagctatat ttcaacctgc taacaatgct ttgataatgt
     2521 caagctgttc tagaagtaag ctaggagttg taggaagcat aaattccctt gtaagaaatt
     2581 taggacaagt tataggtatt actatttcta caacactttt atataatttt atgagtatta
     2641 aagctggata tagggtcaat gattatgtga ttaataatga taaaatattt gtatttggaa
     2701 tgagaaatgt ttatattatt gttacacttg tttgcttaat aggtgcaata ttaattggct
     2761 tttatttatt taaatataat aaaaatgaac aatagttttt ataattatat tcaaattatg
     2821 attatattat aagaaattta ctaaaatact tcctttgagt taaatttaat ttggacatat
     2881 tgacttaaaa atgttttatt aatttgataa aatatttctt ttcctatagt ttataaataa
     2941 aaatcagctg agcaaaatat ttagtatatt atgctcaact gattctttta ttatttaact
     3001 aaaacttcaa tattttcatc ttttccatct ataactatag tacatccatt atacacatct
     3061 ccagcaataa gtttctttgc aattatagtt tctaatgtat ttcctatata tctcttaaga
     3121 ggtcttgctc catatacagg gtcatatcct tctctcacca taacatcttt tgctgaattt
     3181 gtaacttcaa tagtaatatc tttttctttt aatctatttt ttaaatcttt cataaatata
     3241 tctataattt tcttgatatt ttcttggtct aaaggcttaa acattataat atcatctact
     3301 ctgtttaaaa actcaggttt aaatctatgc ttcatttcat tcattactaa attgttagta
     3361 gtctctgtta tatttcctcc tgcttctaat agataattac ttcctatatt agaagtcatt
     3421 attataagag tatttttaaa gtctactgtc ttacctttat tatcagtaag tctaccatca
     3481 tctaatatct gtaagaacat gttaaataca tcctcatgtg ctttttctat ctcatcaaat
     3541 agtattacag aataaggagc acgtctaact gcttctgtaa gttgaccacc ctcttcatat
     3601 cccacgtacc ctggaggagg tccaactagt cttgatacag catgtttttc catgtattca
     3661 gacatatcta ttcttattat attatcttca ctatcgaata aatttctagc aagagttttt
     3721 gcaagttcag ttttaccaac acctgtaggg cctaagaaaa taaatgaacc aattggtttt
     3781 ctttcatctt ttaatcctgc tcttgcacgt attactgcat ttgaaactgc tgtaactgct
     3841 tcatcttgtc caataactct tttatgaagt tcatcttcta gtttaagtaa tttttctctt
     3901 tctccttcaa ctagtttagt tactggtata cctgtccact ttgatacaat gctagatatt
     3961 tcttcttcag taacttcttc ttttaaaaga gaattttcag aaccatcatt catattttct
     4021 tcgtactgct ttatttgttc ttctaactta ggtatttcac catatttaac ctctgctgct
     4081 ttgttgaaat catattctct ttcatacttt tcagctttac ctttagcttc gtctaattga
     4141 gcctttaagt ttttaatatc taatatttga ctcttttctt tttcatattt tgctgtcatt
     4201 tcatcatttt tagattttaa ctctgctatt tctttttgaa tatcatccaa tcttgattta
     4261 cttttatcat cattttcttt aagtaatgct tctctttcag tttctaatgt aaataacttt
     4321 cttctaacaa catctaattc tgttggcaat gaatctattt cactacgaat catagctcca
     4381 gcttcatcaa ttaaatcaat tgctttatct ggtaaaaatc tatcttgtat atatctatga
     4441 gaaagttttg ctgctgctac aatagcatta tcatgtattc ttactccatg atgtatctca
     4501 aatctttctt ttaaacctct aagtatagat attgtatctt caacagtagg ttcttctgca
     4561 attacaggct gaaaacgtct ttcaagagcc ttatcttttt ctatatactt tctatattca
     4621 tcaaaagtag ttgcaccaat acagttcaat tcacctcttg caagcatagg tttaataaga
     4681 ttacctgcat ccattgaacc ttctgtttta ccagctccaa caatagtatg aatctcatct
     4741 ataaacagta taatttttcc atcagaactt tgaacttctt ttaaaactgc ctttaatctt
     4801 tcttcaaatt cacctctata ttttgcacca gcaattaaag ctcccatatc aagtgagaat
     4861 acaatcttat cttttaaacc ttctgggaca tctcctctta ctattctctc tgctaaccct
     4921 tctacaattg cagttttacc tacacctggc tcaccaatca atacaggatt attttttgtt
     4981 cttcttgata aaattcttat aatccttctt atttcttcat ctctcccaat tacagggtct
     5041 aatttatttt tcttggctaa atctactaaa tttgttccat atctagcaag tgcatcatat
     5101 gtgccttctg ggtcttgtgt ttctactctt tgactacctc tcacttgtga taaaatattt
     5161 aaaaagttat tcttatttat attatattgt tttaatattt taccaacagc tgattttgat
     5221 tctgtttcca tcattgcaag cataacatgc tctacactta tatatgaatc tttaaattct
     5281 ttagaaatac tctctgcctt tataagcacc tcatttattt ttctagtagc tgatactcct
     5341 tgtgagtcag caccttctcc atataccttt ggaagcttat caatttcaaa gtttacagta
     5401 tttctcaaag aatcaattga tatattcatt ttttcgatta tatttggtat caatccatcc
     5461 tcttgattaa taagtgctga gaaaagatga attacatcta cttgttggtt atgatttttt
     5521 actgcttcgt tataagcctc atttaaactt ttttgaactc ttaaagtcat tttttctacg
     5581 tccatataaa tccctccatt ttaatattat atccaaataa attaaattct ctcatttata
     5641 attattgatt ttctaagctc taaaagacta tcaagtaaat tctcgttttt ttgtatatcg
     5701 aaactcaatg ttaaattaaa tgttccatct aaattaaaaa aatcgaatac ttcttttgat
     5761 tttgctatta aatacaagtc ttcatctata agtctttttt cacctattaa taaatactga
     5821 catttatcta taaaactctt tttaaaatca atagtgtata gattttctaa aatattatat
     5881 tgataaatag caatattatt aaacactttt aatatgtttg aatttacaaa attgtaaatt
     5941 tgattattgt tatacagtga tatttgttcg tatatatatt ccctaaataa aggatgaatg
     6001 ttttgcttaa gttttaaagt aacttcagct ggtatttcaa aataaagctt ccagtactta
     6061 gagttatttt tttctaaaat aaaagctact ttgttcaaac tggtagaact aatctccttt
     6121 agtgtacaat taaaattata gtgtctcaaa ctagtttttt cattctcaaa tacatagtca
     6181 aaccactgta gaacatttct atcaagtttt aaactcatat aaaaaccttc ttccttattt
     6241 actaacctta ttaggtcttt ttatctgatt atattctgct aataaacttt ctttatcagc
     6301 tctactacct ttatatgcta tatgtggatt tatcatatac tcaccatttt tcactctttg
     6361 taaaaaatcc atttttttca gctccgtaaa caatgaagat aaagaagttt ttggatattc
     6421 aaattcacaa gataattcat ataatgtttt tctaatctta ttatttttat ccatagagtt
     6481 tattaatttt aatggaatta aatccctctt agtatacatt ccttctacta ctttaatata
     6541 attcttaaaa caaacattca tatacttact tttattcata attaaacttt tgctctctgt
     6601 atattcctga tttaaatcct tttttaatgt cttatccaaa ttaaatatct cttctcactc
     6661 tccttatctc taattgataa tataaatata ttacaaaaaa atatgtatgt caaaactgaa
     6721 aatgcaaaaa gttcacctta taagtaaact tttatttagc tataagctaa actttattat
     6781 ttttttaatt tagtatttcc aatttattgg caggattttt gaacaatatt tataaatttt
     6841 ttaaaaaaat ttaaatctat ttaatatggc agactttcat aaaatacatt a
//
LOCUS       DAMJZE010000191         6839 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJZE010000191 DAMJZE010000000
VERSION     DAMJZE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.195"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..1240
                     /locus_tag="PC541_003330"
     CDS             <1..1240
                     /locus_tag="PC541_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2840751.1"
                     /translation="SEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEIKEIDEY
                     PMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILENIKDRYN
                     NYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLVQTIVMN
                     DELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTNFILRRS
                     IIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQRTRWAK
                     GNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISKLVEISI
                     PINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFVAIKGMI
                     EYLKDIIFKREVKWYKTERF"
     gene            1263..3344
                     /locus_tag="PC541_003331"
     CDS             1263..3344
                     /locus_tag="PC541_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2840752.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            3381..4466
                     /locus_tag="PC541_003332"
     CDS             3381..4466
                     /locus_tag="PC541_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2840753.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            4520..5644
                     /gene="wecB"
                     /locus_tag="PC541_003333"
     CDS             4520..5644
                     /gene="wecB"
                     /locus_tag="PC541_003333"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2840754.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            5722..6696
                     /locus_tag="PC541_003334"
     CDS             5722..6696
                     /locus_tag="PC541_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2840755.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         2847 a          643 c         1141 g         2208 t
ORIGIN      
        1 atcagagtat ttgttcgtat ttagtttgtt tagtatatgg tctttattgc ttataaatat
       61 tattttagct atgggtggat atatatttta ttttaaaaac tttgataaag aaataaaaga
      121 aattgatgaa tatcctatga tatcaatact tgttcctgct cataatgaag ctaaagtgat
      181 aggtagaact gtggagtctt tgctattact caattatcct aagagtaaga tggaacttat
      241 tgttataaat gacaattctt ctgataatag taaagaaatt ttagaaaata taaaggatag
      301 gtacaacaat tataatttca ctataataaa tacagatagt ttaacaggtg gaaaaggaaa
      361 gtctaatgca ttaaatattg gatatacgat ttcaaaagga gattttatag ctgtttatga
      421 tgcagataac acaccagata aaaatgcact tagatatcta gtacaaacta tagtaatgaa
      481 tgatgaatta ggtgctgtta ttgggaaatt taggactcga aataaaaata aaaacctatt
      541 aaccaaattt ataaatatag aaacattaag ctttcaatgg atgtcacaag ctggtagatg
      601 gcaacttttt aatctatgta caataccagg aactaacttt atattgagga gaagtatcat
      661 agaagaaatt ggaggatggg atagtaaagc aattgcagaa gatacagaaa taagttttag
      721 gatatataaa ttgggataca agataaagct tgtaccacaa tctataacat gggaacaaga
      781 gccagaaact gttaaggtgt ggataaagca acgtactaga tgggcaaaag ggaatatata
      841 tgtattaatg aaatatataa aaaatatatt taaacaggga agaaataaga ttgtgtttga
      901 tatagcttac tttttttctg tatattttct atttttaaca tctgtaatta tatccgatat
      961 tttatttgtt ttaagtatat ctaaactagt agaaatttct attccaatta acttttttct
     1021 aatatggata ctatcttatc ttttatttat tatagaagtg agtataagcc ttactataga
     1081 aaaaggtgaa gcaacaattg aaaatatatt catagtggca ataatgtatt ttacatactc
     1141 tcaactatgg ctgtttgtag ctataaaagg aatgatagag tatctaaaag atataatttt
     1201 taaaagagag gttaaatggt ataaaacaga aagattttga ttcatgtaca ggaggaatta
     1261 acatgaaaaa atttattata tcaattatat ctttagtatt gtttttttca aatatatcac
     1321 ttatatacaa agtaaatgct gatgagacta aagttaaaaa ttataagttt gaaagagata
     1381 taactattga tggtgttata ggaagtaata gtactttttt tgaagtaaat aaaaattggg
     1441 atattgaaga agttttattg catcttaatt ttagtaagag ccaaatatta aatggagatg
     1501 tttcaagttt aactgtattg ataaataatg taccaataaa atctattaaa ttaaatgcta
     1561 agacaaatta taaaaataca ttagaagtat tagttccaaa agattatata attcaaggat
     1621 ataatgaaat aaaaataaaa acatacaaaa caatatctga taaaatttgc caagatgatt
     1681 ccaatactgg gaactggatg gttattcaca aagaatcata tatatcaatt agatataagc
     1741 agaaaaaggt ggaaaactca ataaatgaat atccgtatcc atatgcagaa attgaaaata
     1801 atcataaatt agatacaact atagtagtac cagataatat gactagagga gagactacag
     1861 cagtgtttaa tttggcatca gcatttggaa aaataacaaa aaatgatgat ttaaagttag
     1921 atgttaaatt atattcagaa atgaaaaatt ggtcagatga taatattata tatattggta
     1981 aacctgaaaa tacagcagaa gaaattttgg atattttaag tataaaggaa caaaccctat
     2041 tatcttcaaa ctgtataatc aagcaagtag attcaccata caataagaat aagaagatga
     2101 tggtggtaat tggtagtaat gaagatgatt taataaaggc atcaaattta ttaatagaaa
     2161 atagactttc taaccaagtt ttatcatcat cagtacttgt aaacaaagag actaatataa
     2221 aaattaatag ggaacaaaaa ttaaatttag gtcatttaac attaaaagat ttaggatatt
     2281 ctgacttttt gttggaagga gcatttaatc aacaagcttt gtttgatgtc aaaataccaa
     2341 cgggtaaggt attagatgat ggttcaaaga ttatcttaaa tttaagatat tctgacaatt
     2401 tagattttga aaaatcttta gttacagtgt ctataaatga tgttattgtg ggaagtaaaa
     2461 aattggatag gtcacattct aataatgata agttagaatt aaaaatacct aaagatatag
     2521 ataataaaaa ctattatcaa gttaaattga catttaattt gagtataaaa aattcgaatt
     2581 gtgttactag agagagtaat aatccttggg catatgtatc aaataattct tatttggcgt
     2641 tatctacgaa agaaaatgaa accttatctt ttgaaaatta tccgtatcca tttgttagag
     2701 atgatgaatt taatgattta actgtgataa tgccagatta ttcagggtca caagctatga
     2761 cctggatgtt tagattaggt gttactttag gtgcgaatat aaattcacat aatggaaata
     2821 taaatgtaat tagaggaaaa gaatttagtg acaagtataa ggatactaat attgtagttt
     2881 ttggagtgcc acataataat tctgttataa agatgttaaa taataattta aatataaagt
     2941 tcgataaaaa ttactccaat tttatatcaa atgataagat aagctttatt gatgattatg
     3001 gaaaaaatat ttctactatt caattaataa aatcaccata taataatcaa aaaaatatca
     3061 tggtaattag ttcaatgaat gaaaaaaact tatatttagg aatggattat ttgttgaata
     3121 aaagtaaagt taatgattta aaaggagata cattgataat tgatgaatac ggagaggtag
     3181 aagatttagc ttataattta aaatctaaga aagaagtaaa agattcaagt tggaatatga
     3241 gtataaataa gacaactaaa gtatttttaa tgatttcttt tattactata attatagtca
     3301 tgatattatc aatgctatat ataaaaaaat ataaaagaag ataatatgat aaatttatta
     3361 ttttaaaaga ggtatataat ttgaggaaat atatgaatga atttaaatta aaaagaagta
     3421 ttttaatctc attaataatc ttaattttta tatttatagt ttactttgta agtattgagt
     3481 taaaagatag gagtctagta aataatcaat ataaaggtaa gattaaatcg gccaatttat
     3541 ctgtagatta tgaaataaat caagttatga aggatataga taaattggga ttgaatacag
     3601 taaatgtacc tataattata aatgtagagt ccattaattc tgatattatg tcaatagata
     3661 ataatagtaa agaaaaggca attaagttaa taaaaaaatt aaataaaaaa ggtatttcaa
     3721 caatattaga ggcatatcca tggattgaaa atggaaaatt gtatgagact gactggaacc
     3781 ctataaataa aaaaagattt ttttatactt ggcaaaatgc catattaaat gaacttataa
     3841 ttgatgtagc aaatccactg gatgtaaatg ttttaaatat tggttctaat tttgtacatt
     3901 tggaagaata tcaacaaaat tggggtgaaa taatagattt tgtacaatcc aaatttgacg
     3961 gtcttgtaac gtacagaact aattggtggt atacaaaaaa agatgattta agtagtaagt
     4021 tgttttatga acaaaagctg aacaataatt tttttgataa gttagatttt atatctatag
     4081 cagcatattt tgagttaagt aataaacctg ttaatacagt ggatgaatta atttctgcct
     4141 tacactcatc tactgtaaac aatagaggac aaaatataaa acaagaaata tataaccttt
     4201 ataaagctca tagaaaacct atattttttg gagaattagg tttttcaaat agagaaagtg
     4261 caagttctca gccgtggaat catactcctt caaaagctgt aaatggcgaa gaacaagcaa
     4321 ggtgttttga ggcatataag aaagtttttg aaaatgaaga ctggataaat ggtttttctg
     4381 tgttttgtgt aggtaaaata gatgacgaaa aaaattttta cccatcaaaa gagagtataa
     4441 aagttattaa gtcttggtat gaataatttt gaaagtgtag aatttatata gattaaatag
     4501 atgattagga gagatataaa tgaatgacat caaggttatg actgtatttg gaactagacc
     4561 tgaagctatc aaagtagcac ctttaataaa ggaacttgaa aaaagagaga atataaaaag
     4621 tattgtttgc gttacagctc aacatagaga aatgcttgac caagttatag aaacctttaa
     4681 tataaatgta gactatgatt tggatataat ggaaaaagga caaagcctga atgatataac
     4741 atgtaagatt ttaaataaac ttcccttgat tttaaacaag gaaaatccaa atataatatt
     4801 ggtacatgga gatacaacca caactctagc tacaagtttg actgcatttt ataataaaac
     4861 tcttgttggt catatagaag ctgggcttag aacctatgat aaatattctc cttttccaga
     4921 ggagctaaat agacaactta caggtattat tgctgatatg cactttgctc ctactaattt
     4981 ggctagaaaa aacttgattt ctgaaggaaa acctaataat aatatatttg taactggtaa
     5041 tactgctatt gatgctttaa agatgacaat aaaagaaaac tataatcatc ctataattga
     5101 tgagattggt aacgatagga tgattttgtt aacctctcac agaagagaaa atttgggtaa
     5161 acctatgaaa aatatattca gagctataaa gcgaatagta gatgactttg aagatgtaca
     5221 gatagtatat cccatacatt taaatcctaa aatacgaact atagctgatg agatatttgg
     5281 aaaatttcct gaaaaaattc atattataga gcctttagat gtagctgatt ttcataactt
     5341 tttaaataaa tcttatatga taatgactga tagtggagga atacaggaag aagctccatc
     5401 attaggtaag ccagttcttg tgttgcgtga taaaacagaa agaacagaag gaattgaagc
     5461 aaagacactt aaattagtag gtactaatga agatagaata tataattcag tgagcgattt
     5521 attaataaat aaagataact atgttcagat gagtaaagca tctaatccat atggagatgg
     5581 aaatgcaagc aaatatatag tggatataat aattaaaaaa tttaattgca aatatttaaa
     5641 ttagataagg cctaaaacat taactaattg atataagatt tatgataata ttaaggaata
     5701 caatttttaa gggggagtaa tatggctaga aaattaataa aaaatttggg taaaagtaaa
     5761 agtgtaaaga gagtgaaact tttatttaag aagattttta ttactgtatt catagtagca
     5821 agtatagttg ctatttttaa tataacgaaa tattttgagg aattatacaa agtaagggat
     5881 ttaaaaagta ctaaaattga atactatatg gatgtggctg atgaagcagg agatggaaag
     5941 gtccaattaa gttggaaggc cttacttgct atagacatgg taatacatga tgaagattta
     6001 agtaatataa aaaagaaaga cacattggat ataggggaaa agtttatagt agaagataaa
     6061 aatggtaaag gcgaaaaggt gtataaggta aaaaagttta ataaggtatt aagcgaattg
     6121 aaatttgact cttctcaaaa aagtagagca agaaaataca tgaaagattt agaatacaca
     6181 taccttggaa ataaacaatt agatagtagt gatgaaaaaa ttaaatttat aaagaagtta
     6241 gaagactcag ctataagaga atatattgat tatggaatat tgccctctat aacaattgga
     6301 caagctatat tagaatctgg ttggggaaat tctaaactta caaaacagag taataattta
     6361 tttggtataa aagcagataa agcatggaaa ggaaaaagtg tagaaatttc aacttcagag
     6421 cattataatg aaaaaattgt agctagtttt aggtcgtata attcattaca agattctgtc
     6481 aaagaccaca gtttattttt aattaataat aaaagatata gaaaacatgg gttgtttgag
     6541 gcaaaagatt atattagtca agctcaagca ttagaaaatg caggatatag tacagcagaa
     6601 gataaaaaag gaaatcgtat atatgcagag ctattaatag atgttattag gagttataat
     6661 ttgcaattga tagataataa agttgagaca aaataattgt taactttata tgaggagata
     6721 acaagataag atttttttct acaaaaagaa tagaaaaatt gtaaaaaaaa ctaaaaaaat
     6781 gtattgactt gttatttaaa aggtgatatt attattactg tactcgaaag agaacaaaa
//
LOCUS       DAMJZE010000192         6772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJZE010000192 DAMJZE010000000
VERSION     DAMJZE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.196"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            84..425
                     /locus_tag="PC541_003335"
     CDS             84..425
                     /locus_tag="PC541_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2840756.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            576..989
                     /locus_tag="PC541_003336"
     CDS             576..989
                     /locus_tag="PC541_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2840757.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1191..1832
                     /locus_tag="PC541_003337"
     CDS             1191..1832
                     /locus_tag="PC541_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2840758.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1856..3109
                     /locus_tag="PC541_003338"
     CDS             1856..3109
                     /locus_tag="PC541_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2840759.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3155..3805
                     /locus_tag="PC541_003339"
     CDS             3155..3805
                     /locus_tag="PC541_003339"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2840760.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3856..4452
                     /locus_tag="PC541_003340"
     CDS             3856..4452
                     /locus_tag="PC541_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2840761.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4465..5304
                     /locus_tag="PC541_003341"
     CDS             4465..5304
                     /locus_tag="PC541_003341"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2840762.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5549..6703
                     /locus_tag="PC541_003342"
     CDS             5549..6703
                     /locus_tag="PC541_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2840763.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
BASE COUNT         2804 a          635 c         1189 g         2144 t
ORIGIN      
        1 atcagtctta catatatttc cgatagatta tattgataaa aaacactaaa attaaattaa
       61 taaaagtact tggagggatg gaaatgaaag aggaaaattt tattaatgtt gaatacgagg
      121 ataaacgttt ttcaattaat agtaaagaag ttttgtacat agaaaagcaa gagtatgaag
      181 taaagattta tactgaaagt ggtaattatg aagttggaga aaatgctcct tgtataagaa
      241 aactattgaa agaagaaaat tttttcaaat gcagtaatag ttatattgta aatctagaca
      301 agtgtgaatt ttatctcagc aataattgta tttttgtgaa taataatttt ataagaattt
      361 taggttcaga aagaaaaaag aaattaaaaa agaaattaga aaaattatat gattatgaag
      421 attaatattg ggaggctgta ttttatacag cctctttatt tattgattgt attattaata
      481 aattttaaat tatgataaaa tataaaacaa gtaacttatt tcagaataaa atacttttta
      541 aaagaaaggg acgagatatg ggagattata ataaaatgct tgaattaaag aataacttag
      601 gtatcagtga aagaaagtta aaatatcctt gtatctataa acatttcaaa gggaaatatt
      661 atgctacaat gggtcttagc aaggctattg atgatataga aaacatatgt gaaatatatg
      721 gtaaagaaaa tttaatccaa aacagaaata aatataaatt agttattaga catactgaaa
      781 gagaagaaga tatctatgtt tatagggatt tagatggaaa cttttatcat aaaaaagaag
      841 aagatactaa tgatttagtt ttatacaaga ccttatatga tgatacagga atatttgcaa
      901 gacctttgga tatgttttta gaaaaagtag atacagataa gtatgtcaat tcaattcaag
      961 aatacagatt tgaagaagta tataaataat ataagagaaa tatgtttata aatttagaaa
     1021 aattctttaa cagtacaata atttgcagaa atacaaatga gatttattgt aaataatatg
     1081 ttagattaga ttataaaata gaaacaaagt aaagtattat atgatataat taagttttga
     1141 gaagtgataa tttatattta ataaaaaata ggcatatatt aggaggacat atgggaaata
     1201 ctttttatat agtaagacat ggacaaacgg attggaatat attgggaaaa acacaaggtc
     1261 atggtaattc tgaccttacg cctcaaggta tagaacaggc aaaagaactt tctgaagata
     1321 ttggcaagta ttcaattgat tatattttta gtagcgacct tgggagagca atgcaaacag
     1381 ctcaaatttt aggagataag ctcaatattg aagttcaaaa aactgaggca cttagagaaa
     1441 tgggattcgg agtttgggaa ggccttttaa taaaagaaat tcaaaaagat tattcagata
     1501 tatatgcaac atggagaaat gaaccacact tagtaaatat accagaaggc gaaactctta
     1561 agattataaa ggaaagagta gatgctttta taaaggaact aaatgaaaaa tatgataata
     1621 aaaatataat tcttgttaca cattcaatta cattgagggt tatgttgttg tcatttttag
     1681 aatcaggtat ggaaaatata tatagaatta agcaagataa tactgcttta aatatagtag
     1741 aatttaaaga ttatgggcct gttatagtaa aaatgaatga tacaagtcat ataaaaaatc
     1801 atgtaaagat aaataattct gcactggagt aggaatcaat tataaggaga aatttatgag
     1861 taaagttata gtagttggtg gaggagcttc tggaatgatg gcagctttgt cagcttcaaa
     1921 aaacaataat gaagtaatat tagttgagag aaatggagaa ttaggaagaa aacttagggc
     1981 aactggtggc ggtagatgca attttaccaa taatagagag atagaagatt tttttgataa
     2041 agttgtcagt aataaaaaat ttctctatag tagtttttat acatttacaa ataaagacct
     2101 tatttcgtat tttgaaagta gaaatttgga atataagatt gaagaagaaa atgaccacaa
     2161 agtatacact aaaaatgata aatctataga agtaatagaa gtattaaata aagatttact
     2221 caatcacaat gtaaagataa tgtataataa gaaagttata gatattataa ctgaagaaat
     2281 tgctctaaaa gatgattcta ataaggacaa gtcaaagtat ctaataaaag ggattatttt
     2341 agataatgga gataaaatac taggagacaa ggttattata tcaactggag gagtgagtta
     2401 ttcaaagacg ggttcagatg gttcaatgta taagattctt aaaaagcatg gacatacatt
     2461 aaacaaacta tatcctgctt tagttccact tacaatagag gaaaaatgga ttaaagattt
     2521 acaaggaata tccatgaaga atgtggaaat atcttgtaag attaagaaaa ggaaaatatc
     2581 aaaatctgga gacatgctat ttgcccattt tgggataaca ggaccttgtg ttcttataat
     2641 gtcttcttat attaacaaga ttattgaaaa ggaaaaggta gaattaaata tagatttctt
     2701 accaaattta agtactgatg aaatctctag tataatcaga gcatttccaa ataaaaatgt
     2761 tctaaataat cttaagcaaa tactacctca aaatttctta aaagagattt tttctctatt
     2821 atctttagtt gataaaaagg caagtgattt gtctaaggca gatgaaatta gaattattga
     2881 gtatataaaa aatatgaagc taacttgtaa tgggactaca ggtataaaca ctggtatggt
     2941 tacttctggt ggtatttcag ttaaagaaat taattcttct actatggaat ctaaattagt
     3001 taagaactta ttttttacag gtgaagtaat tgatatagat gcagaaactg gtggatataa
     3061 tttacaaata gcattttcaa ctggatatct tgcgggtata agtgtataaa tataattttt
     3121 taataattaa atatgaataa atagaggtgt atatatgggt aatttagtta tagctgtaga
     3181 tggtccagca ggagctggaa aaagtacaat agccaagata gtagcaaaaa agctaaatat
     3241 aaattatata gatacaggtg ctatgtacag agcagttact tataagtgtt taaaaagtgg
     3301 tattgatgtc aataatgaaa aagaagttat tcaaatagct gaaaattcgg atatagattt
     3361 taaagataac aatatatatt tggacaaaga agttataaat gaagaaataa gaacaataga
     3421 agtaagcaat aatgtttcta atgtagcaaa aataaaagaa gtaagacagt tgatggttga
     3481 agttcaacga aaaataggaa tgaaaaattc agtaatacta gatggtagag atataggttc
     3541 atatgttttc ccagatgcag attacaaatt ctttttagta gctactccag aagaaagagg
     3601 aaatagacgc tataaagaac tttgtaataa aggatataat actactttag aagaagtaat
     3661 tgaggatata ataagacgag atgaaattga ttcaaataga gaatttgcac cattggtaaa
     3721 agctaatgat gcactagaaa tagatactac aggaaaaact atagaagaag ttgtagaaga
     3781 agttgtatct aaaataaatc tttagttttt tatgcatcat aatgaaaata aatgtagtgt
     3841 tgaagggaga taaacgtgaa tttttataga tttgtaataa atatttttaa gggtttttct
     3901 aaaatatttt ttaaatatga agttataggt gcagagaata ttccagatag aggaaatata
     3961 gtaatagcat caaatcataa atcgaatctt gaccctatat tcttggctgc tgccatagaa
     4021 aatagagaaa tagctgcaat tgcaaagaaa gaattgttta aagtaaaacc attgggattt
     4081 atattaaaaa aattgcatgt tatgcctata aatagagaaa aaccagatgt atctacaata
     4141 aaaactattt taaggtcagt tagagacggt tatgtattag gaatatttcc agagggtact
     4201 agaataaaag gagattcttt tggtaaggct aaggcaggtc tttctgtatt tacaataaaa
     4261 agtaaatcta aagttgttcc agtatcaata atatctaaat ataaactttt tagtaaagta
     4321 attgtatata ttggagaacc aatatcattt gaagaacatt ttaaggaaaa attgagtaat
     4381 gatgaccatg aaaggatatc acaagagatt ttagaagtga taaaacaaaa ttactttaaa
     4441 tattcaaaat aagggggtaa agttatgaac ataaaaatag caaaaaatgc tggcttttgt
     4501 tttggagtaa aaagagcaat gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc
     4561 atatatgctt taggtcctct tattcataac aagcaagcag tagcaaaata tgaagaaaaa
     4621 ggtcttaaga ctgtgaatga aattgataca ataccaaatc atgaaaatat gataataagg
     4681 tctcatggag ttcctgaaaa tatatataag gaagccaaag ataaaaaatt aaaaattgtg
     4741 gatactacat gtccatttgt aaagaaaata catacagtag taagtgaata tcataataaa
     4801 ggctatgaaa taatagttat tggagatatg aagcatccag aagttatagg tataaatgga
     4861 tggtgtgaaa attcagctat aataataaag accttagaac aaatggaaaa tatggaattt
     4921 gataactcca aaagatattg tcttgttgca cagactacaa taaatccaga gttgtatata
     4981 agtatagtta ataaactatc agataaatta gaagaaatag tatttaatga tacaatttgt
     5041 tcagctacaa aaacaagaca agaatcagct aaagagttag ctaaagaagt tgattgtatg
     5101 atagttatag gaggtaaaca cagttcaaat actcaaaagt tagtaaaggt ttgtgaagac
     5161 ttagtaccaa cttttgcaat agagactaaa gatgaattgg atgtaaatac gcttaaaaaa
     5221 tataaaaatt tgggtattac agcaggggca tctacaccta attggataat agaagaagtt
     5281 gtaacattcc ttgaaaattt ataatttgat aggtaataaa aataggtgga cattgttata
     5341 atttatcata atatccacct ttgatttcat attacatgtg gtaaatagat aacctaatat
     5401 caaaaagtgt gtatcttaag atgaaaaact aagagtataa gatgcgcact ttttaatatt
     5461 agaaattcaa aagaaatatt gatattgatt atcaatagaa aatagaatat aatataattg
     5521 ttgcaaaatt agtctaggag gtaaattaat ggaaatgttg atatccgctt ctgaagaggc
     5581 gtttttacat gtaggttcta tgattggatt ttttattttg ttatttggat atattaatta
     5641 taaaactagt ggaaatttta ctaatatcat atcaaaaaac agaaaatttc aacccttaat
     5701 aggtgcttta attggagcta taccaggttg tggcggaagt ttggcaataa tgccattgta
     5761 tataaatgga aaattaagct ttggagcaat aattgcaagt ttaatagcta gtatgggaga
     5821 tgcagcattt gtacttattt catcaaatat taaaatgtat ttttttgtca ctatagttag
     5881 tacaattaca ggaataataa ctggacaatt agtggattac tttaaactag aagaaaaatt
     5941 agggttaaaa aaccgtagaa aatcagataa atatgatata agtaaaaata accctaaaga
     6001 aaataaagaa caacataatc atgatgaaat aattttagac actctagcta aatctcatgg
     6061 aaatacaaat agacttgcat ttataataac acatggaaag ggatataaaa tttatatagg
     6121 aattatagta atcggtttta tatttatgtc acttgctcac tctggactta atcttcctat
     6181 aattgagaag ttacattcat tggaagaagt aatagcagtg attggaatat tattttcaat
     6241 aatttatatg tggtgtttta aaaaggtatt taaaaattct aatcaaaaag aggaagaaaa
     6301 taaaaagatt tctttgcgag aaatgcttat ccattctgtt ggagaaatat catttgtaat
     6361 tacatggatt tttattgcat atcttattta cgatttgata atattaattt taggtggaga
     6421 tgaatactta gttaaattag tattatcaac aggtgtaata agtgtattta taggtgcagg
     6481 tcttggactt attccaggtt gtgggataca aattgtgctt atgtcatttt atttgaaggg
     6541 aactatacca cttggtgcag tgatagccaa ctctatttct caagatggag atgcactttt
     6601 cccactttta gcaatggata aaaaatcttc tttatgggct atgattatta ctactatacc
     6661 agcaatactt gtaggtatga tagtttatgc atttttaggt taaatataat tatgaataaa
     6721 gaagtattaa aaaaataata aagcacataa atttatttat aagaatagtg aa
//
LOCUS       DAMJZE010000193         6760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJZE010000193 DAMJZE010000000
VERSION     DAMJZE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.197"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            210..1043
                     /locus_tag="PC541_003343"
     CDS             210..1043
                     /locus_tag="PC541_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2840764.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRVPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1136..2068
                     /locus_tag="PC541_003344"
     CDS             1136..2068
                     /locus_tag="PC541_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2840765.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2120..2602
                     /locus_tag="PC541_003345"
     CDS             2120..2602
                     /locus_tag="PC541_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2840766.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2614..3483
                     /locus_tag="PC541_003346"
     CDS             2614..3483
                     /locus_tag="PC541_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2840767.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3526..4281
                     /locus_tag="PC541_003347"
     CDS             3526..4281
                     /locus_tag="PC541_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2840768.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4323..5144
                     /locus_tag="PC541_003348"
     CDS             4323..5144
                     /locus_tag="PC541_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2840769.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5149..5910
                     /locus_tag="PC541_003349"
     CDS             5149..5910
                     /locus_tag="PC541_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2840770.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6071..6328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6441..6680
                     /locus_tag="PC541_003350"
     CDS             6441..6680
                     /locus_tag="PC541_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2840771.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         2982 a          633 c         1147 g         1998 t
ORIGIN      
        1 taaaaaacac tatataatac tcaattttca acattctata caattataca gaaattatac
       61 agcataataa tcaccgaaat aacaaatttt aaaaattaag caaaactttt tgtcgaagtt
      121 caacgtctat atatatgtag tgtaaatttt tgggataaaa tactacaaaa atataaatgt
      181 aaaaatatag taatattgga ggtcgaacga tgagaaataa aaagaaaaaa ataaatagaa
      241 agaagttgta tttgctttta agtgctattg tagtgtgtct ggcttttata gtagttggtg
      301 ttcgcgtcag ctctttaaat atgaaggaaa atgaaattag tagaaatgca aaactgtcaa
      361 gttctactat tacagatata gtatctaata catcaataga agttagtaaa gggccaagta
      421 aaagttctgg aaaaatagca tacataacta tagatgatgg tccatctaaa tttactgacc
      481 agatgataaa aactttaaat aaatacaatg ttaaagcaac tttttttatg atagatggga
      541 atatgaagga atatccacag caagttaaaa atataattaa aaatgggaac acagcaggtt
      601 ttcatagtgt atcacatgat attcacaagc tttatgttac tagtacatca gcaaaagagg
      661 aatttgatac aaatgatcaa actttttata agataactgg taaacattca aaagtaataa
      721 gagtacctta tggaagcaaa ccatatacac cacaagcctc ttatcaggct ttagttgatg
      781 ctggatataa aatttgggac tgggacttag atacagaaga ctggagatct aattcttctc
      841 aaattgtaca aaatgtaaaa aatcatatta agaatagaaa aggtgaagat aaagaccaac
      901 ttgtagtttt aatgcatgag aaaaaacaga gtgcagaagc tttagattca gttttaaaat
      961 tcttatcaga tgaaggttat gaatttgcgc cagtagatca aaaccaaata ccaaaaaatt
     1021 attggttacg taatttagag tagttaaaat ataagaatat tgattgatat ttttagaaat
     1081 tgagaataaa aatgagtgag gtgtaggtat attgaataaa aaactttata taggaatggt
     1141 cggaatattg tctttgatga tggttggttg taataaaagt ttagcaaaag taaatgatgt
     1201 ggaaatcaca aaagaacagt acaaaaaaac aaaagcagtt ttatctgcta ctaataatta
     1261 tataaatggg cagtctttag atgaattaga aaaaactttg gataaaaaag gtagaaataa
     1321 attagaaaat gttataatat cgtttatggt ggataatgaa cttttatatc aagaggctaa
     1381 ggataaagga ctaactccaa gtaaaagtga agtagactca aaatatcaag aactagaaga
     1441 taagatgaat ttgaatacaa gctataagga aaaaatggac aaagcaggtg ttgataaaga
     1501 ttatttaaaa caagaaatat ctagagattt agcaatagat aaaaataaaa aggcctttga
     1561 agatagaata aacataagtg ataatgatat ggaagcctac tatacaagtc ataaaaaaga
     1621 ttttaatgta gaagaagtaa gtgcttctca gatacttata tcgactttag ataaaaataa
     1681 aaaagaagta agcaaagata aaaaagaagc tttgaagaag aaggcagata atatattgac
     1741 taaaatcaaa aatggagaaa gttttgaaag tttggctaaa aaatattcgg atgataaagc
     1801 aactggaaaa aatggaggac agctaggata ttttacaaag gatgataaaa atgctgagtt
     1861 tacaaaggaa gtgttcaaat taaagaaaaa tgaagtttca aatgtgtttg aaactagtta
     1921 tggatatcat atagtcaaag ttactgataa aagagaaaga caaaaaagtt ttaatgagtg
     1981 tcagagttta ataagagaat ctattttaaa tgaaaagtat attgagcata taaaaaaact
     2041 gaatgaagat gctaaaatag acagatagaa tttctaacag ctcaatccta gcctgtaatc
     2101 ggagggagat tttacaggaa tgtttaagag tttaaagaga acgaaagttg agaaatacat
     2161 tatcgataat aaagactctt tttatagaat agcatatagt tatacgaaga atgaagaaga
     2221 tgccttagat gtggttcaag aagccatgta taaggcacta tactcagtgg aaaatattaa
     2281 agaagtaaat tatataaaga catggtttta taaaatttta gttagaacga gtatagattt
     2341 tattagaaaa aaccgtaaat ataataatat gacagatatt gaccttatag atgagactgg
     2401 agagtatgat aaatatactg atttggattt aagaagagct ttggaagaat tacctattga
     2461 atacaaatct attataatat taagattttt tgaagattta aagattgaag aagtagcaat
     2521 aatattggat gaaaatgtga atactgttaa aactaggtta tatactgctt taaaaaaact
     2581 aaaattaaaa attgaagagt agggataaaa agaatgacaa gcaaagaaag gttaaataag
     2641 ttaaaagatg agtatcataa aattccaata cccaagaaat tagatactat aataaatcat
     2701 gaaaaaatag aaaacatata tcgagagaaa aataaaaaag taaatagatt aagatttaaa
     2761 gttgcaatag cctttgcatg tatatttaca gtattagtaa atataagtcc tgtatttgca
     2821 gacaattttt ctaagatacc tgtaattgga gcaatagtag aagtaataac tataaaaaat
     2881 tatagtttaa aaagtgaaaa ttatgaagct gagattgata ttcctaaaat acgaggttta
     2941 aaagataaaa acttagaaca aagattaaac agtagtttta tggaagatgg aaaaaggctt
     3001 tatcatcaat tccaagagag aatggagaag atacaatcat caaagaataa aggatataaa
     3061 tctttaagtt tgagttatag tgtaaaaaat aattctaaaa aatttctatc aatagagatg
     3121 acaaaaaatg agatagaggc ttcatcatat gtgagtaagg tacattatac tattgataaa
     3181 aaaagacaaa tagttttgac tttgccaatg ctttttaaag atgataagta tataaaagtt
     3241 ataagtgata atataaaaga acaaatgaga gaacaaatga aaaaggatag tacaaaatcc
     3301 tattttatag atcaaaaaaa agacttgcct gttgaagatt ttaaaacaat taataagtat
     3361 caggattttt attttaataa aaatgaagac cttgtaattt gttttgatga atatgaagta
     3421 gcacctggtt atatgggcgc agttgagttt gtaataccat ataaagtaat aaaagatttg
     3481 taaattgaat agtagtttat atataaatct tttaaggagg acaatatgac tacatataaa
     3541 aaacttaaaa aatctatatt taaaattgct atattttcaa tatgcttatt agcttcatta
     3601 gtagatacaa accctatatt tgcaggtagt tcctttaatg aaacttcaaa tagtgatacg
     3661 ataaaggtgt taaatgctaa gagtatcaac tttaaacaag acaattatga agtgaatgtg
     3721 agtattccta aaataaaagg tttaaaagat aaaactttag agaataaatt aaacaatgag
     3781 tttttgcaag atgggaaaaa aatttacagt gaatttcaaa ataaaatgaa aaaggaagat
     3841 aataagggtc atagatacct aaatgtaatc cataatgtca aaaataattc taaggacttt
     3901 ttatcaatag aaatgacaaa agaagagata gaagcttcct catctataac taagaagcat
     3961 tacactgttg ataaaaatag aggaatacta ttaaatttac caatattttt taaagatagt
     4021 agatatatta gagctataag tgataatata aaagagcaga tgagaaatca aattaagaaa
     4081 gatgatacaa aatcttattt tatagaccaa gataaagact cgccagtgaa agattttgaa
     4141 tctataaata agtatcaaga tttttatttt aataaaaatg gaaatcttgt gattagtttt
     4201 gatgaatatg aagtagctcc tggttatatg ggagcagtag aatttgtgat accaaataaa
     4261 gtaataaaaa gactaagata gatatataga agatttttag atagactggt aaggagaatt
     4321 atatgaatat atatgaacaa gcaataagat taatagaaaa taatgaggat ttcgcatttg
     4381 ctacaataac gtctcattca ggttcaacac cacgtgaaac tggagcaatg atgattgtga
     4441 aaaatgattc tactatattt ggctcagtag gaggaggtag tgtagaagca gaatgtataa
     4501 aacatgcaat taatgttata aaaaacagag aatcaatgtt gtataaattt actctaaaca
     4561 aatctgatgt tgcaaaacta ggaatgatat gtggaggtac tggagaaata caaatagatt
     4621 ttatagatag taaattaaaa agtaatatag aaaaatttaa taagagacta aaggaaaata
     4681 caagtaaagc atatatattt ggtgcaggtc atatttcaag agatgttgct gtgatactat
     4741 ctcttttaga atttagaaca gtagtaatag atgatagaga agaatttgct aatcatgaaa
     4801 ggtttccaga atcagaagta gtagttttag attcttttga aaacatacca gattttccaa
     4861 ctgacgaaaa tagttatata attattttaa caagaggtca tctatatgat tcaagtgctt
     4921 tagaatgggc tcttaaaaga gaggctggtt atataggtat gattggaagt agaacaaaaa
     4981 ttggattgac ttacgaaaaa ctcatgaaaa aaggttttaa gaaggaagaa ttatcaaaag
     5041 tacatgcacc aataggcata aaattgaatg ctcaaacacc agctgaaata gctgtatgta
     5101 tagctgctga acttataaat tgtagagcta ataaggaaaa ataaactaat gaacacaggg
     5161 agtaaagata ttgatacttt aaaggttaat tttagaggga ttgaaataaa agcagtttta
     5221 gtatataaaa atagaaaaaa tatttctata aaaatagatc catttggcaa aataactgta
     5281 atgagtcctc ctaaaatatc caaaaaaata ataaaagata taattattga aaaaggagac
     5341 tggatactta aaaagtcaga aaaatgtaaa ggaagagagg aagtatataa acaaagaatg
     5401 tttataactg gcgaaaaatt tctttattta ggtgaagagt accatttagt tataaaagaa
     5461 ctgttgagag attctgtcaa aaaaagtaac tgtagaataa ctattaacga atatcaaata
     5521 gttgttcaga caaatgatac aagtacagat tttattaaaa aatctttaaa atcttggtat
     5581 aagatggaaa gtgaaagaat agtattagag agaatagatt ttcttaaatt aaattgtgaa
     5641 ataatgaagc aattagttcc agcgagtgtt aaggtaaaag aacaaaataa gagatgggga
     5701 agttgtacag ctcaaaaaaa tatttatata aactcaaaaa tatctatggc aagattagat
     5761 gtaattgatt atgtaataat acatgagttt agccatttag tacatatgaa tcactctaaa
     5821 aaattctatg atttagtaaa atccattatg cccgattata aagataaaga aaattggcta
     5881 aaaataaatg gatataaaat aataatctga aaacgattat tccaaaatat aggcgtaaag
     5941 tataaaagaa gtattattgt gaaaaaaata aataatctga aaattttaaa aaaagtcatt
     6001 gacattgtaa atgataggga atataatcta tattaacaac aaaacagaat taaaatataa
     6061 aattaaaaaa ttaaaagcaa tgacagagac aacaatacta atagtgttat ccacagagaa
     6121 ccaatgttgc tgagaattgg tgttaacagt agtataatga tcactctgga gcttttgtac
     6181 tgaatatttt tagtaagtac aaacgtatgt cagcgttaca tgactaaggt taaggtaatt
     6241 aattacccga cctgaaaaaa gaggggtaga tgtatttttt atatctacaa ataaggtggt
     6301 accgcggaat ataactttcg tccttataag taatatttat attgcttata aagatgaaag
     6361 tttttttatt ttaagcgata taaataaatt taatccatat aaaattaata aaaaaataat
     6421 aaaaataagg agggattttt atggaaaata ctgtatatgc taaattaaac caaggaatag
     6481 attctttttt aagagtggct atgactctaa gaagaagaga ggttgatata caatcaataa
     6541 gtatgactgt agataatatg aataattcag gtataaaatt aatagtaaat gaagaaaaga
     6601 catccttaga ttctgtctta aaccatatga aaaaactaca tgatataaga gagataactg
     6661 ttgaaagaat gagacattaa ttaaatatat tttataaagc taaagataaa tatgcttatt
     6721 ttaaaataaa aaactaaaga taaatattaa ataagaaaaa
//
LOCUS       DAMJZE010000194         6510 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJZE010000194 DAMJZE010000000
VERSION     DAMJZE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.198"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     misc_binding    100..311
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            474..1763
                     /locus_tag="PC541_003351"
     CDS             474..1763
                     /locus_tag="PC541_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2840772.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1817..2896
                     /locus_tag="PC541_003352"
     CDS             1817..2896
                     /locus_tag="PC541_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2840773.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2917..4461
                     /locus_tag="PC541_003353"
     CDS             2917..4461
                     /locus_tag="PC541_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2840774.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4624..5166)
                     /locus_tag="PC541_003354"
     CDS             complement(4624..5166)
                     /locus_tag="PC541_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2840775.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5314..5598)
                     /locus_tag="PC541_003355"
     CDS             complement(5314..5598)
                     /locus_tag="PC541_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2840776.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5661..6425)
                     /locus_tag="PC541_003356"
     CDS             complement(5661..6425)
                     /locus_tag="PC541_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2840777.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2265 a          808 c         1126 g         2311 t
ORIGIN      
        1 catataaaac taaaaaatgc aagtaattga ctttttaagt tgacaaaata ttgcatatat
       61 attatgatat aaatcataaa ttaactacaa atcaatatta tatattcgta gaagaagaaa
      121 gtagcataaa ggaattttac agagagttag atttggctga gaactaatat tggaatttat
      181 gtgaaaagga ctttggagaa tactattgta attatgttag aatagtggtt taattcgcct
      241 taagaaaatg agtgagtaaa attaattttt actaataaga gtggtaacac ggatttatat
      301 tcgtctcttg gatttttcca agaggcgttt tttattataa acttatttca gaaaatttaa
      361 aactatatat taaatgaaaa ttataaaaat atgatagtta ataaatttat aaattagaaa
      421 attgtaaaat atgctaaggt cagtagtttt acgatttaaa tggagggatg aaaatggaag
      481 aagaaaaaat taactatgct ctggaacaga ttccagagaa tcaaaaacgt ggttgggtag
      541 ctatgttttc agttttagta gcaataggtg tggatttatc atctgttatt ttaggcgctg
      601 agttggcaca gagtatgcca atgaaacaag caatattgtc agtaattgtt ggttcatttt
      661 tttctgcaat tttgtatact acatgctctt tggttggttc atctacaagt ttatctacat
      721 ctatgataac aaagtatgta tttggagaag ctggagcaaa aatattttct ctagttatag
      781 gggtttctct attaggttgg tttggagttc aagtaggttt ctttgctcaa aatgcacaga
      841 taataataaa agatatattt aatttagatg tcagtatgca gattttaagc ctaataggtg
      901 gtctcttgat gatgagtact gcaatttatg gatataaggc aatggagaag ttgagtgtgt
      961 attcagtacc atttttatta gttcttatga tgctaactat attcttagct tttagagcaa
     1021 atggaatttc agttgatgat aatatgaaat ctactatgac atttgcaggt ggagtttctc
     1081 tgtcaatgag tataataata gttggtgcaa ttgtatctcc tgatataagt cgttgggcta
     1141 aaagtagaag agactgtgct ctttcaagct ttttgggaat acaatttgga aatgctttta
     1201 tgataatagt ttcgatagta ttagtaaaat gtatgggaac ttcagatatt atgagaattt
     1261 ttattacatt aggaattgct ataccaggta taatagtact tacattagca caatggacta
     1321 caaatacaag taatgtatat tcagcatcat taagtatagc tcttgtactt aagaaggctc
     1381 ctgagaaagt attaactata gtccttggta ttatagcaac attattggct gtatttggaa
     1441 tatatgaagg ttttatagga tttttaaatt tacttggaat tgtaattgca cctgttggtg
     1501 gtgtatatac agcagaatat tacatagtaa agcaagaact taaaggcttt gataaaggag
     1561 tattatataa accaatagtt aaacgttcaa tagtgtcttg gataattggt atattaataa
     1621 cttacctttc aacatatgga tttataactc ttactactat agcaccttta gatggtttta
     1681 tagcagggtt tgtagtacaa tctattattg gaaaagtgct ttgttcaaca aaaaataagc
     1741 aagaaataga caaagcagta taaagaaatt tgtttataaa gtataaaata aattagaaat
     1801 atgaggagaa agtaacatga gatttttaac agaagaaagt gtagataaaa tagcagttgg
     1861 tgcagcagtg cttggaactg gaggtggagg tgacccatat gtaggtaaat tggttgcaaa
     1921 acaagccatt aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac ttgatgatga
     1981 tgctttggta gttccagtat ctggtatggg ttctcctgta attacaatag aaaaattact
     2041 ttcagaagta gagttaacta cccctttaga aataatggag aaattattaa atagaaaagt
     2101 ggatgttata attcctatag aaataggagg gataaactca cttatgccta tagcagttgc
     2161 tgctaaaaaa ggacttccta tcttagatgc agattctatg ggtagagctt tccctgaagc
     2221 tcaaatggta actttttatc ttgaaggata tgaggcatct cctgccgtaa tgtctgatga
     2281 aaaaggtaat tctgccatcc tttatcctgt ggatggaatt tggtcagaaa gattagctag
     2341 aactttaact gtagaaatgg gaggaagttc ttctattagt gactataatt taagtggagc
     2401 tcaagttaaa aaagctgcta taggtaatac tcttacaata gctgaaacta taggagggtt
     2461 tttacttgaa aataaatctg attcaaaagg tgctgttgag aatatactaa aagaattgtg
     2521 tggatacaag ttatttgaag gaaaagtaat tgatattaag agagagttga aaggtggatt
     2581 tactagagga catgcctttt ttgctgggat taatgagtat gatggagagt attctatatt
     2641 attccaaaat gagaatttaa ttgccaaaaa aggtgataca ccactttgta ttacaccaga
     2701 tttaatagca gtattagatt tagaaactgg atttccaata actacagaga gaataaaata
     2761 tggctctcgt gttatggtag tagcttttcc ttgtaatgaa aaatggagaa cagaaaaagg
     2821 aattgagaca gttggacctg gatactttgg atatgatgtg gaatataaaa cagtagaaga
     2881 attacaagga aaataaaagt ttaggaggaa attttgatgt atcgtgtagg aattgacgtt
     2941 ggaggaacaa atacagatgc ttgtatactg gatggggaat taaaagtaat tcattcagtt
     3001 aaggtggcaa ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa aaaagttata
     3061 gatgaaagta agattgacca ttcattaata aaatatgcta tgcttggtac aactcattgt
     3121 acaaatgcta ttgtagaaag aaaaagactt aataatgtgg ctatgataag aataggaaag
     3181 ccggccacta ctgccattct tccatttata gattggccag aagacttaag agaaaaaata
     3241 gaattagatt caatattggt atctggtgga tatgagtttg atggcagaaa aataaatgaa
     3301 ctttgtaaag atgaagttgt agagttttgc aatagaataa aaggcaaagt ggaaagtgtt
     3361 gcaataagtg gagtatttgc tccagtaaat aaacaacaag aagaagaagt tgcaaaatgg
     3421 gttagagaaa ttttagggga tattcctata tctttatcaa atgaaatagg taatataggt
     3481 atattagaaa gagaaaatgg agctatttta aattctgcat tgtctcaagt aggtaaagct
     3541 gtatctttag gatttgaaaa agcacttaaa gatttaggta tagatgctca aatatatttc
     3601 agtcaaaatg atggaacatt aatgaattta gagtatacaa tgaaataccc aatttttaca
     3661 attggatgtg gaataacaaa ttctataaga ggagcttcct tcttatcaaa agtaaaaaat
     3721 gcaatagtat tagatgttgg tggaactaca agcgacttgg gtgtactata taatggattc
     3781 cctagagaat catctattcc agttacaatt ggaggagtac atacaaactt tagaatgcca
     3841 gatgttttaa ctattggtct tgctggtggt acatgtgtaa aaggcgaaaa agataatata
     3901 aagataggtc ctgaaagtgt gggatataga ataactgaag atgcaataat atttggtgga
     3961 gacactctaa ctttatcaga tgttgttaca aaattagata tggcgtttga agatagaaaa
     4021 agcaatgttg aaaacttaga taaagagtat tgtaatgagg tatataagaa ggttatttta
     4081 aaattagaag atgctgtaga ccaaatgaag actagtcaag atgatgttga attggtttta
     4141 actggtggag gaagtataat tgttcctgaa aaactaaaag gcataagcaa tgttataaga
     4201 cctcctcatt atactgctgc aaatgctatt ggtgcagctt taggacaaat aagtggagat
     4261 gtagaaaaag tatacagttt agataaaatg tcgagagaag atgccataga agatgcaaaa
     4321 aaagaggctg taaataaagc tctaaaagct ggtgctaaag aagatactat agaagtatta
     4381 aatatagaag atgttccact tgcatacctt cctggtaatg cacttcttat aaaagtaaaa
     4441 gttgttggtg atttaattta aagaataaat ataagtattt ccagaattat ataaatattt
     4501 ctggactcat ttttaaactt attttatcaa taaaaacagc ataacttttg actatattct
     4561 tataagtttc cgaacagccg atatatacct attttttgag gtttatcggc tgtttgttat
     4621 cttttatcgc attccaacta tttttcttac cgtatttgct gtacgaattg taatcttatc
     4681 acttacattt gaactggcag tctttgacca ccaatttgtc tttttataat ctttacgact
     4741 aaaagaccaa aagatagcat ttttataatg atatactttt tcatactctt ctttaggatt
     4801 tccaacctca tcataaaact cgtcataaga taagtcagga aacataaaaa taagattatc
     4861 atatatttct ttgttgttat ctccccacca attaggagca ttatttagta tatcttttaa
     4921 ctcttcttgt gaaataataa aaactggtat ttccaattca aactgttttt ttatcattaa
     4981 tcttattgta tttgagataa ccttgctgtc atctacatca cttaaaaaga caacattacc
     5041 actattaagg tatgtagaaa cttctgaata accaagttct acaaagcttg cttttaattc
     5101 agacataatt atcttgttct taccacttat attgatacct cttaataatg cgatataact
     5161 tttcatataa taatttcacc tctaaacatt cacatttttc tatgtagtac aaacttgaat
     5221 ttgcatcttt tataattctt tcaaaacttc ttcatatgta atttttttaa tataaaaaaa
     5281 tcggtgctta atttgtaccg atttttgttt ttcctagtaa agaactcgtt tttttgcttc
     5341 tgctatagct ctagcagcac tttttgcttg cattcttttt tcttcttctt ttttaaattc
     5401 atttattgtt tcatttagat tcttatcatg ttcatttttt tctttatctt cgttttctct
     5461 gtatactaat atataatcta ttattgattc gtttattatc tgagatatac ttttattttc
     5521 attaattcct attctcttaa cttccttaag tagagtctca tcaatatgaa ccgttgtttg
     5581 ctttgtattt tttgccacct taatcacctc ttaaaaaaaa ttctaacatt aaaatttttt
     5641 ttagtcaata aattttaata ttaaaattct ttaaatgttt tatttgattt tctaaaattt
     5701 tctattgtat ttatcatctt attttttata ttttcaccta gtacagaaga ctttagtcta
     5761 ggattttcga tatggtcata aattggttct aaaaattcaa cttcgacagg aacagagtta
     5821 attttaccaa taggttggta tccttcataa gtatcttttg aattttttat tacgagtgga
     5881 acaatagggc attttgcttt atatgctatc tttaaagctc cacttctaaa ctccgaaacc
     5941 aatgaatttg gttctttaat ccatgttaaa tctccttctg gaaacactgc catactttga
     6001 cctgtaagta tattttgaga ggcttgggct atactcttta taccctcacg attgtttttt
     6061 ctgtttacat atacacatct tagtaatttt gcccattctt taaatatagg tatatttctc
     6121 caaactggtt catctgcaat tacacatcca attggtcttt ctacacttgc tgttagtatg
     6181 aagctgtcaa gcatactaga atgatttaca acaaatagaa gtggttcttt tggcagtgtt
     6241 tccttaccaa ttatattaag ctctatattc actatatcta aagatttttt agcttgtttt
     6301 tgcataaatt caaatttttc ctttaagcta aatttatcag gattcttttt aattttaata
     6361 agcctaggta tagaagatgt aaaacctata agttgatatg tagcaaattt taagtaatta
     6421 ttcataaaat tatctcctta aaataaaatt atttattact gtaaagattt taacataaaa
     6481 taaaatttaa gtctatttta attcaatttt
//
LOCUS       DAMJZE010000195         6504 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJZE010000195 DAMJZE010000000
VERSION     DAMJZE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.199"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            397..1311
                     /locus_tag="PC541_003357"
     CDS             397..1311
                     /locus_tag="PC541_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840778.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            1335..1970
                     /locus_tag="PC541_003358"
     CDS             1335..1970
                     /locus_tag="PC541_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2840779.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            2346..3062
                     /gene="pyrF"
                     /locus_tag="PC541_003359"
     CDS             2346..3062
                     /gene="pyrF"
                     /locus_tag="PC541_003359"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2840780.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            3088..4131
                     /gene="carA"
                     /locus_tag="PC541_003360"
     CDS             3088..4131
                     /gene="carA"
                     /locus_tag="PC541_003360"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2840781.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            4184..>6504
                     /gene="carB"
                     /locus_tag="PC541_003361"
     CDS             4184..>6504
                     /gene="carB"
                     /locus_tag="PC541_003361"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2840782.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT         2715 a          669 c         1274 g         1846 t
ORIGIN      
        1 gtatgttttt tgttgataat aatattaaag tagaaataaa aatacttttt tagtgaaaag
       61 ctatacaaga ataccgaaaa aaacttataa tataataatg agatagatat ttataaatga
      121 aaaaaaattt ttctggcaaa gcttaattaa aaaggtaata gctagtgttg ttatttaaat
      181 tagcatagaa aatcaaataa aaaaattatg ctaatgtata gacattgata aggtagaatt
      241 taagcctttt gaatgctata ttaaactaga gtagatattc ttatgaattt caaaataggg
      301 caggaggaaa aaggaaataa aactataaaa aatataaagt atttatataa atttatagag
      361 tttatacgtc agaagatttc agttaggagg gcataaatgg agctttatga agccattttt
      421 tatagaaaat ctgtaagaaa tttttccagt aaaaagataa aaaagccatt gatggaagaa
      481 ataaaaagaa gttgtaaaaa tttagaatac ttaaatgaag atttaaatat taaagcacat
      541 gttgttgata gaggtcatat aatacatttt ttgatgggga aggaatgtaa agtaaaggca
      601 cctcattata tagtagttac atcagacaaa ggtgaggact atttacagaa tataggattt
      661 gctacagaag gtgtagtttt gcgccttaca acattgggaa tagctacttg ctggttggaa
      721 ggaaacttaa agagagaaga tatcttggaa tttgtagata ttgggaataa gaattttgaa
      781 gaagatgagg aagatgattt agattatcta gctggaataa aaacagaaga agatgatgaa
      841 aacaaaatag aaaatcctta tataataata gcatttggat atccagcaaa aaatgaagag
      901 ttgtttagga cgagatatgc tagacctgat agaaagccag taaaagatat gataaataag
      961 ataaatagga atagaaagtg gaaagatatc ttagaattga caagaagagc tccatctgta
     1021 aaaaattctc aaccgtggta tttccataag gatgaacgag gtcttcattt atttgaaaag
     1081 agacctaaga aacactgtga agatatgaat aaggttagct taggtgtagc cttaagacat
     1141 tttgatatag cttgtataaa aaataaaata gatgtttcat atgaaaaact tcctataagg
     1201 aataaaatag gaaaatcata ttttataact gtagtcgagc atgtgaaacc agaagaagaa
     1261 acccaagaag aaaatgtaac tctagaaaaa gaagaatcgc aagatgaata aaattttgga
     1321 aggggatatt ggatatgtat aagtgtggaa aatgtaatct tagaggctgt tctaatggaa
     1381 atatggataa attaatgaag atatgtccaa ctagagaaat tgaactacaa caaaaagcaa
     1441 agcaacttta tgaagaagaa gagaatagaa aaatagctta taatgctgga ttagttgaag
     1501 cagaggggta ttgtcaatat actagactaa aagagattat agagtttgct aaaaagtgtg
     1561 gttataaaaa gattggcata gcattttgta ttggactaaa aagagaaatg caaacagtgc
     1621 aaaaaatatt tgaacatcat gggtttgaag tattttctgt tatatgtaaa aatggggcaa
     1681 tgcctaagag tacaattgga ctagaagcat ctcaaacaat atcaaagtgt tcagatgagg
     1741 taatgtgcaa tccaatagga caagctttat ttttaaatga agctaaggtt gaccttaatg
     1801 taattcttgg attatgtgtt ggtcacgata ctttagccat gaaatatatg gaagctccta
     1861 taacaacttt agttgtcaaa gatagagtaa ctgggcataa tccagttgct gcaatatata
     1921 atgcagaatc atattttgag aaaaaattgt ttgataaaga tgaagaataa ttcaaagact
     1981 tattgacata aaaagcaatt agtggtttaa ttataaaaaa gataaaactt taaattatcc
     2041 tatagataca taaagttata taaataaaga aaaatattac agctaaattc cttaaagtag
     2101 tccagagaga ctaaagggtt atagataatg ttagtatata acctctagta aatcttctag
     2161 aggtttttga tgagtataaa ataaaaattt aaatataata aagtctttta aactagtcca
     2221 gagagactgg gaaagatatc tcatagctaa tgaatagagt tatgtataga taaaatcctt
     2281 atgcctcttt tggtgtgagg atttttttat gattatttag gctttattaa aaaggagatg
     2341 taaaaatgat aaatggtaaa gaaaaattga tagttgcaat tgatactgac gaatttgata
     2401 aagctaaaga actaatagac agattagaag atagtgtaga catatttaag gtagggctag
     2461 agcaatacgt tgccacaaaa ggtaaaacaa tagattatct taaagaaaaa gggaaaaaaa
     2521 tatttttaga ccttaaattc catgatatac caaatactat gaaaagtgca gtgaaagctg
     2581 cagtgagaga taatgtttgg ttaatgacaa tacatgtatc agatatggaa ggaatgagac
     2641 aatgtgcact aattgctagg gaagaagcag aaagattaaa tatagaaaag ccattagtag
     2701 ttggagtaac agttttaact tcattaagca accaagattt acaggacata ggatgtaata
     2761 tgacaacaga agagttagct ataaagagag ctaaactggc aaaagaatca ggaatagatg
     2821 gggttgtttg ttcagcgcaa gaagtagata aaatagtaga agcatgtgga actgatttta
     2881 taacagtttg cccaggaata agacctaaat cagcagaggt tggagaccaa aaaagagttg
     2941 taactccatc agatgcaata aaaaaaggtg ctcattattt agttgttgga agacctataa
     3001 caaaagcaga aaatccaaga gaatcagcta ttaatattgt aagagaaata gaaaatgctt
     3061 agataaaata tttgggaggt agcttacatg aaggggaaat taattttaga agatggaaca
     3121 gtatttgaag gaaaagcatt tggatatcta aaggaagcta ttggagaagt tgtatttaat
     3181 acttcaatgg ctgggtatgg agaactattg acagatgcaa cttattatgg acaaatagtt
     3241 actatgactt atccacttat aggaaactat ggaataaatt tagaagatgt tgaatcaaat
     3301 aaggtgcaag taaaaggact tattgtaaga gaaaaaagtg ataacccaaa taattttaga
     3361 tgtgaaatgg atatagacac atatctaaag caaaataagg taataggact tgaaggaata
     3421 gatacaagag cattaactaa aattattaga aacaatggaa ctaagagagg aattataact
     3481 ttagaaaatg tatcattaca agatgttcag tctaaattag aagagcattg taacaaagat
     3541 tcagtaaaaa atgttacaag aaaagaaatt gaaaatataa aaggtactgg agctaaagtt
     3601 gtagttatag actttggtgt taaaaatagt gtactagagt ctcttaaggc ttgtaactgt
     3661 gatataacta tagtaccagc aatgactaca gtagaagaaa ttttaaatat aaatcctgac
     3721 ttaatcttat tatctaatgg cccaggaaat ccagaggata taaatgatat agttgaaaat
     3781 ataaaaaaat taataggcaa aaagccattg acaggaatag gtttaggaca tcaaatttta
     3841 gcattggcat taggaggaaa gacttcaaag cttgtatttg gacatagagg aggtaatcac
     3901 ccagttaaat gtttagaaac tggaaaggta tttataactt ctcaaaatca tggatataat
     3961 gtttcagaaa tgccagaaaa tacagaagta actcacataa atttaaatga caatactata
     4021 gaaggaatga gacataagga attaccaata tatagtgttc aatacattcc agaatacaca
     4081 acaaaagaag atttagattg tatatttaat aaattcttag cattaatata gatttagtat
     4141 tatttacaga tagtaaaaac catttagata aggagaaaaa tttatgccta aattagatag
     4201 tataaagaaa actttagtat tagggtcagg accaataata ataggtcaag ctgcagagtt
     4261 tgactattca ggaacacaag cttgccagtc tttgaaagag gaaggaattg aagttgtatt
     4321 aataaatagt aatccagcca ctataatgac tgataaagaa gtggcagata aaatatatat
     4381 agaaccatta acaatagaat ttatagaaaa aataatagaa aaagaaagac cagacagttt
     4441 acttgcaggt atgggtggac aaacagggct aaacctagca gttgaacttt atgaaaaagg
     4501 catactagat aaatatggag taaagataat aggtacttca gtagaatcta tcaaaaaagg
     4561 tgaagataga gatatattta gagaagtaat gaaagaaata aatcagcctg taatagttag
     4621 tgatatagtt acagatttac aagcaggtct tgattatgca cttacaattg gttatccagt
     4681 agttgtaaga ccagcataca cactaggtgg aactggtggg ggtatagctg ataatgagga
     4741 agaattaaga gaaatacttt cacatggtct acaattaagt cctgttggac aagtattaat
     4801 tgaaaagagt ataaaaggtt ggaaagaaat agaatatgag gtaataagag attcaaaggg
     4861 aaattgtata gttgtatgta acatggaaaa tatagaccca gtcggagttc atacaggaga
     4921 cagtatagtt gttgctccaa ctcaaacttt aagtaacaaa gaatgtgcaa tgcttaaaaa
     4981 agcatcttta gatatattaa atgcagtaga agttcaaggt ggttgtaacg ttcaatttgc
     5041 acttaatcca catagttttg aatatgcagt tatagagata aatccaagag ttagtaggtc
     5101 atcagcttta gcatcaaaag ctacaggata tccaatagct aaagtttcgt caaaaatagc
     5161 tttaggttat actttagatg agatagaaaa tgcagtaact aaaaaaactt atgcttgttt
     5221 tgaaccaact atagattatg tggtggcaaa aataccaaaa tggccttttg ataaatttaa
     5281 aaaagcaaat agaaaattag gaactaagat gatggcaact ggtgaaatta tgagtatagg
     5341 aagtaacttt gaggctgcta ttcttaaagg gataagatca cttgaaacag gaaaatattc
     5401 attagttcac acaccatctg aggagagaag cattgaggaa ttaaagaaaa gagttgtagt
     5461 acctgatgat gagagactat ttgatttagc tgaaatgata agaagaggct ataaagtaga
     5521 aatgatagaa caaattacag gtatagataa atggtttata aataaattta aatggatagt
     5581 agagcaagaa gaaaaattaa aagttatgaa aattgaagat ttaactaaag actatcttct
     5641 tgaattaaag aaaaagggat tctctgataa aggtatatct gacctaatga agataagtcc
     5701 agaaaaatta tatgaattga gaagtctata caatataaaa ccagcatata aaatggttga
     5761 tacttgtgca ggtgagatag atgctatatc tccttactat tattcaactt atgagcaata
     5821 tgatgaggta gttgttagtg ataagaaaaa ggtgatagta ttaggttctg gtccaataag
     5881 aataggtcaa ggtatagaat ttgactattg ttcagttcac tgtgtaaaat ctttaagaaa
     5941 aatggacata gaaactataa tcataaacaa taaccctgaa actgtaagta cagactttga
     6001 tacatcagat aagctgtatt ttgaaccatt aacagaagaa gaagttttaa gtataataga
     6061 aaaagaaaag ccagaaggtg taatactaca gtttggtgga caaacagcta taaaattggc
     6121 taaattttta catgaaaaaa atatacctat attgggaact gattttgatg atatagatgc
     6181 agcagaagat agagaaaaat ttgatgattt actagagaga ctagatataa atagaccaaa
     6241 aggaaagggt gtatggtcac ttaacgaagg tattgaagtt gctaatgagt taggttatcc
     6301 agtattagtt agaccatctt atgtacttgg tggtcaaggt atggaaataa cttataacga
     6361 agaaaaatta tctcaatacc tacaagatgc atttgataga gaccataaaa atccagtact
     6421 tatagataaa tatctaactg gtagagaaat agaagtagat gctatatgtg ataaggaaga
     6481 tatattgata cctggtataa tgga
//
LOCUS       DAMJZE010000196         6447 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJZE010000196 DAMJZE010000000
VERSION     DAMJZE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.200"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..898
                     /locus_tag="PC541_003362"
     CDS             <1..898
                     /locus_tag="PC541_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2840783.1"
                     /translation="DNKKVDTQIAKVITDLQDNDYITWKKDATLEASAGLVFTGGTNG
                     EAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLFVEFTKRMRDKVGAKFQTVLYKK
                     SDADYEGVVSVENKIKDKDLVESSLIYWATGAIAGCDINKSNTNKKYDGEFDVGVNYT
                     QIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTDDKNDDFSSNQSVRVLDQIANDI
                     ATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNMRAIEDFKADDVSVEPGSDKKTV
                     VVSDAVKVISAMSKLYMTVSVS"
     gene            920..1390
                     /locus_tag="PC541_003363"
     CDS             920..1390
                     /locus_tag="PC541_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2840784.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            1443..1700
                     /locus_tag="PC541_003364"
     CDS             1443..1700
                     /locus_tag="PC541_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840785.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            1705..2145
                     /locus_tag="PC541_003365"
     CDS             1705..2145
                     /locus_tag="PC541_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2840786.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPILNKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            2145..2339
                     /locus_tag="PC541_003366"
     CDS             2145..2339
                     /locus_tag="PC541_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840787.1"
                     /translation="MYEGDYDSNIAHYCLHKFKWKPHEYMDLPVDEKAFVAASIDIKV
                     EEEKEEAKKADKDAKRGRRR"
     gene            2407..3198
                     /locus_tag="PC541_003367"
     CDS             2407..3198
                     /locus_tag="PC541_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2840788.1"
                     /translation="MGLFSKKNKKPCCICGSEKGLMPSIEGENFCTACNCKYIDFSEN
                     ILKVTSIMKMMSNSEGMKKFIEVEKKNLKLLEKFTETKSINSSISFDEEQNLLKISYK
                     NRNQILVEKIIKFDDILEFELLEDGETIVKGGLGRAITGGVLFGGTGAVVGGITGKKT
                     SRKVVEIFKIKITVKDINNPIEYINLINSKVKTNSSIYQKAFSDAQEILSILSIITKY
                     NVMEDKKKSISSSTADEILKYKNLLDMEAITQEEFDAKKKELLNL"
     gene            3265..5607
                     /locus_tag="PC541_003368"
     CDS             3265..5607
                     /locus_tag="PC541_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2840789.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMNNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIRESDNQQRRLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLSMINDGQLSDGGLNKMIFQSAERSRASYLDTAK
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTLGQIFTSVKNNAVMVFGAIQKKIEDTVSSRGFRTFITDVKDSLYVLGAVGFNV
                     FSGFINLLSSPAFQNFFNVMIVGTSLVVQGLGWIITQALSVANVFSRNWSIISPIILG
                     VVSAISTYIIALGIMQAVTIAGTVANWMSWTSEVIRQRGILGTARALAIGKAEQWGFN
                     AAIYACPIFWIVLGIIAVISAVFILVAVYNHFAGTSISAISLIVGAWYWLCAVVYNVI
                     VGIVNAINICVVEIANIFRTGLYAVQCFFIDMANAGLKAGVNLDKAFDKFATNLANGI
                     IKAVNIAVKGLNWLVQQINKIPGIDLPQMKEFQKVNTVIGTKTTFKPIQKPPEPKAWK
                     PELVEYKNLKSEFIKGYDVGHQLQNKFKDTFDISKIAEKAKKDLGLDDLWDKKYGLGD
                     GLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVN
                     IKIDMNNTNNISKDADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            5623..6324
                     /locus_tag="PC541_003369"
     CDS             5623..6324
                     /locus_tag="PC541_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2840790.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDADNGSDVKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPTPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     VPKYTIYKGQVLKLG"
BASE COUNT         2526 a          744 c         1317 g         1860 t
ORIGIN      
        1 agataataag aaggtagata ctcaaatagc taaagtgatt acagacttac aagataatga
       61 ctatatcact tggaagaagg atgcaacact agaagcaagt gcagggctgg tatttactgg
      121 tggaactaat ggcgaagctg tgacaggagc agagtaccaa gctttcttgg ataaaataga
      181 aagctatagc tttaatgctt taggatgttt ggctacaaca acagagatta aaagtttatt
      241 tgtagaattt acaaagagaa tgagagataa ggtaggagca aagtttcaaa cagtactata
      301 taagaaaagt gatgcagatt atgaaggtgt agtgtctgta gaaaataaga ttaaagataa
      361 agatttagtt gaatctagct tgatttattg ggctactgga gctatagcag gatgcgatat
      421 aaataaatct aatactaata aaaagtatga tggtgagttt gatgttggtg taaattacac
      481 tcaaatacaa cttgaagaag cactaaagag tggtaaattt atatttcata aagttggtga
      541 tgaagttcat gtgttagagg acataaatac ttttgtatca tttacagatg ataaaaatga
      601 cgatttttca agtaaccaaa gtgttagagt acttgaccaa attgctaatg atatagcgac
      661 tttatttaat acaaagtact tgggtgaagt accaaatgat aaatctggtc gtatctcgtt
      721 ttggaatgat gtagttaagc atcatgaaca actgcaaaat atgagagcaa tagaagattt
      781 caaagctgat gatgtttctg tagaacctgg aagcgacaag aagactgttg tagtaagtga
      841 tgctgtaaaa gttattagtg ctatgagtaa gctttatatg actgtttcag ttagttaaca
      901 ataagaaagg agaataataa tggcacaaca aataaaagca agagatacaa taagtgcatc
      961 taaggcagag tgttttgtaa ctataaaagg taaaagatat aattttatgc aagctattaa
     1021 cttagaagct aaaatggaaa agaataagag tgagatacct atattaggta gtactacaaa
     1081 aggaaataaa tcaacaggaa gtaaatattc aggaaatgca acattttatt ataatacctc
     1141 tatatttaga gaattgttgt atgagtataa agaaactggt gaggatattt acttcgatat
     1201 acaaattacc aatgaagacc caacaagttc agtgggtcgt caaactataa tactggaaga
     1261 ttgcaatatg gactcaggca taattgctaa atttgatgct gatggggagt atttagatga
     1321 agatatggat tttacttttg agaattggaa attagttgag aaatttaata tagcaaatgg
     1381 tatggagtaa aatacacatt tatgatttat gtatgtgtat tttttattta taagaatagg
     1441 agatgattaa aattaaggat aaatatgaga taaaagattc aatttctttt gattatagca
     1501 ataaaagacc tttggaagaa cgtgtcagcg agatgtataa aaaggcagga aaatatctta
     1561 tagatatttc agataagtta gcaacagata caattgatgg ttcgtcatta aagccaataa
     1621 tcataaaatt tgaaataaat gaagctggtg ttgcaacaat agaaaaacaa acaaaatatt
     1681 tggtgatgga ggtagaataa gaatatggga gatttaaacg cttttttaag tcaaaatgca
     1741 ataaaagtag agaatagaaa gtatgtggca agtgaaaggt ttataggaga agatggaaaa
     1801 gcaatcgaat gggaacttaa agcaatagat tcagataggg acagacaatt aagaaaagat
     1861 tcaactataa gagtacctat attaaataaa aaagggaaag ctacaggaca atacactagt
     1921 gaaacagatt ttaatactta cactttgaaa ttatgtgtgg aaactatagt atttccggat
     1981 ttacatgatg cagaattaca aaatagttat ggtgtaatgg gtgcagagga gttattaaca
     2041 acaatgttga ctcctggcga atatacagac ctttcaagtg aggtaggaga ggtaaatggt
     2101 tttgatagaa cttttgaaga taaagtagaa gaagcaaaaa actaatttat gaaggtgatt
     2161 atgattctaa tatagctcat tattgccttc ataagtttaa atggaaaccg catgaatata
     2221 tggatttacc agttgatgaa aaagcatttg ttgctgcttc tatagatatc aaagtagaag
     2281 aagaaaaaga agaagctaaa aaagctgaca aagatgcaaa gagaggtaga agaagataat
     2341 ctctggtgta aaaattttac taatatagta taatatattt aaaaattgta ctgggggatt
     2401 aaataaatgg gattgtttag taaaaagaat aagaaacctt gttgtatttg cggaagcgaa
     2461 aaaggactta tgccatctat agaaggtgaa aatttttgta cagcttgtaa ttgcaagtac
     2521 atagatttct ctgaaaatat tttaaaagta actagtatta tgaaaatgat gtcaaactca
     2581 gaaggaatga aaaagtttat agaagttgaa aagaaaaatt taaaattgtt agagaagttc
     2641 accgagacaa aaagtataaa ttcttcaatt tcttttgatg aagagcaaaa tcttttaaaa
     2701 atatcatata aaaatagaaa tcaaatatta gtagagaaaa taataaaatt tgatgatata
     2761 ttagaatttg aacttttgga agatggagaa actatagtta aaggtggttt aggtcgtgct
     2821 ataactggag gagtcttatt cggaggtaca ggggctgtag ttggaggaat tacaggtaaa
     2881 aaaacaagta gaaaagttgt agaaatattt aaaataaaaa taactgtaaa ggacataaat
     2941 aatccaattg aatatataaa tttaattaat agtaaagtaa aaacaaactc ttctatttat
     3001 cagaaagctt tttctgatgc tcaagaaata ttatcaatac tatcaataat tacaaaatat
     3061 aatgtaatgg aagacaaaaa aaagtctata tctagttcta cagctgatga aatattaaaa
     3121 tataagaatt tgttagatat ggaagctatt acacaagaag aatttgatgc taagaaaaaa
     3181 gaattgctaa atttataata tataagcact tacttaaaaa taagtaagtg cttatattat
     3241 gttaaaaaag aaaggaggtt aaaaatggca actatacaaa catctatccg aattttcgac
     3301 ggaatgacac ctgcttttag acacatgaat aatgctatga atattgtatt aagttcattc
     3361 gagcaattac aaagaacatc tagcaatgct atagatgcta atagtattag aacagctaga
     3421 gaagaactag cacgtgcaga agctggcttt gatagactag aacaacaaat aagagaatca
     3481 gataatcaac agcgaaggct taatgaggat ataaataagg gtgcaagttc tacagataga
     3541 ttagttggaa gtgcaaagaa gctagcagca acctacttag gtataagaac gttaggaggt
     3601 ctaggaaatt taagcgacca gatgacaagt actaacgcga gacttagtat gataaatgat
     3661 ggtcaactct cagatggagg attaaataag atgattttcc aatctgctga aaggtctcgt
     3721 gcatcttact tagatactgc aaaaatagtt tcacgaatag gcatgaacgc aggtaaggcg
     3781 tttagcagta caaaagaaat tgtaggtttt gcagagcaac taaataaaaa gttcgtaatt
     3841 gctggagcaa gtactgagga aatgaattcg gcattgttac agctaaccca agggttgagt
     3901 tctggtgtat taagaggtga ggaactgaat gctgtgtttg agtcagcacc taacatcatc
     3961 caatcgattg cagattattt ggacgtggac ataggaaaaa taagaggaat ggcatcagag
     4021 ggaatgttaa cagcagatat tgtaaaaaac tcattacttg cagcagcaga gcagactaat
     4081 gcagagttcg agaagatgcc ttatacgcta ggtcaaatct ttactagtgt aaaaaataat
     4141 gcagttatgg tatttggagc tatacagaag aaaattgagg acacagtttc aagcagggga
     4201 tttcgaactt tcataactga tgttaaagac tcattatatg tacttggagc agttgggttt
     4261 aatgtattta gtggatttat taatttactg agtagtccag cttttcagaa tttttttaat
     4321 gtgatgattg ttggaactag tttagttgta caagggctag gttggataat aacacaagca
     4381 cttagtgttg ctaatgtatt ttcgcgaaat tggagtataa tttcacctat aattttaggt
     4441 gtagtttcag ctattagtac ctatataata gctcttggta ttatgcaagc agttactata
     4501 gctggaactg tagcaaattg gatgtcgtgg acatctgaag ttattagaca aagaggaatt
     4561 ttaggaacag ctagagcttt agcgataggc aaagctgaac aatggggatt taatgctgct
     4621 atatatgcat gtcccatttt ttggatagtt ttaggtataa ttgcagtaat atctgctgta
     4681 tttatattag tagcagttta caatcacttt gcaggcacaa gtatttctgc aatcagtctt
     4741 atagttggag cttggtattg gttatgtgca gttgtttaca acgtaattgt agggatagta
     4801 aatgctataa atatctgtgt ggttgaaata gcaaatatct ttagaactgg attatatgca
     4861 gtgcaatgtt tctttattga tatggctaat gcaggactca aggcaggtgt taatttagac
     4921 aaagcttttg ataaatttgc taccaatcta gcaaatggta ttataaaagc tgttaatata
     4981 gctgtaaaag gattgaattg gttagtacaa cagattaata aaattccagg aattgattta
     5041 ccacaaatga aagaatttca aaaagtaaat actgtaattg gtacaaaaac aacatttaaa
     5101 cccattcaaa aaccgcctga gcctaaagca tggaaaccag aattagtaga gtacaaaaat
     5161 ttaaagtcag aatttataaa aggatatgac gtaggacatc aattacaaaa caaatttaaa
     5221 gatacttttg acattagcaa gatagccgaa aaagcaaaaa aagatttagg tctagatgac
     5281 ctttgggata aaaaatacgg acttggtgat ggattgggtt cagctggatt aaattcacct
     5341 ttgagtgacg cagcaaaagg agcaaaggac acagcaggca atactgcaaa aatggcaaag
     5401 actatggata aaagtcaaga ggacttaaaa tatcttagag acattgcaga gcaagaaaca
     5461 ataaaccgat tcacaggggt aaatataaaa attgacatga acaacactaa taacataagt
     5521 aaagatgcag atgtggatgg tatagttaat gtactaacag aaaaactgaa tgatgctatg
     5581 gttgtatctg ctgagggaat agtttagaga ggaggtgtaa tcgtggctta tgatttttat
     5641 ttagatggag tacaattacc aatacctcca ccaaagttag agattaaagt tacaaataaa
     5701 aataagacag ttgatttgat aaatactgga gaagtaaaca tattaaaaaa agaagggcta
     5761 tctgaaataa gttttgaagc agagtttaca cataataaat taccttttta tcgtggaact
     5821 tttagggatg ttcaattctt tttaagtaag ctagaattac taaaaactga ttgtaagcca
     5881 tttcaattta ttgtatcgag ggaaatgggt ggaaaagtac tatttaacac taatatgaaa
     5941 gtgtcattag aagaatataa catagtagaa gatgcagaca atggttcaga tgttaaagtt
     6001 gcaataaagt taaagcaata tagagattac tcaactaaaa aattagttcc tgcaactcct
     6061 gaaaagacaa actatggtag gactcccact ccagtcatga aaccaaaaga atttagacca
     6121 gattcatcca ataagccaaa tggtaaaaca tatacagtaa aagcagggga tagcctttgg
     6181 tcaatctgcc agaagcaact tggtaatggt tcattatata agaaagtata tgagttaaat
     6241 aaaacaatga tggataaagc taacaagggt aaaaaagtac ctaaatacac aatttacaaa
     6301 gggcaggtgt taaaacttgg ctgatgaatt agttctggca aatgataggg atgtaaggct
     6361 agttattgca cattgggaag atttctacga accagttgtc cttgatggta tcacatggga
     6421 gatagaaaga agaggaacac cttctaa
//
LOCUS       DAMJZE010000197         6416 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJZE010000197 DAMJZE010000000
VERSION     DAMJZE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.201"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            343..1617
                     /locus_tag="PC541_003370"
     CDS             343..1617
                     /locus_tag="PC541_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2840791.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1824..2141)
                     /gene="trxA"
                     /locus_tag="PC541_003371"
     CDS             complement(1824..2141)
                     /gene="trxA"
                     /locus_tag="PC541_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2840792.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2408..3160
                     /locus_tag="PC541_003372"
     CDS             2408..3160
                     /locus_tag="PC541_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2840793.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3147..4028
                     /locus_tag="PC541_003373"
     CDS             3147..4028
                     /locus_tag="PC541_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2840794.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4254..5609)
                     /locus_tag="PC541_003374"
     CDS             complement(4254..5609)
                     /locus_tag="PC541_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2840795.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2469 a          931 c          816 g         2200 t
ORIGIN      
        1 ttactatttt tacaacttta ttagtcatat tcataattat ttctaattag tgtttaacat
       61 tttatgtaaa atttataaaa aaatgtatat aaattaaaat aagtgactat actaaaaata
      121 tgatacggat aaacaataag ttcaacctat ctcccccaaa atcaagtgag ctacagtttc
      181 ccccaactgt agctcatttt cgtgtaattt aataaacatc tcaacaagaa ttaattaaca
      241 aaacccctta taaaaacata acatatatta aacaagctct tgattaaaac ttgagctttt
      301 ttaataaaaa acagaatagt ttattagata aggggttttt ttatgcaatt catggatgta
      361 tctaaataca ttgaaggtgc aaaccttaaa gttaaaacta aaagtggtga aactgttaaa
      421 agaatatatt ttaacaactc tgccactcca ttagttttaa aaaacgtagt ggataactta
      481 aactgtgaga ttcctttgct tacttatatt aatgctccag gaataatctc agaaaaaaat
      541 actttaaaat atgaaaatgt acgctctact atcctcaaac ttattgatgg agacgaagaa
      601 aaggattctg ttatttatgt caaaaatgct acagaaggaa taaacttctt agcaaaattt
      661 tttaaagatg aaaatccaga taaatttgtt attaccacag caatggagca tatggctaat
      721 tacctgcctt ttaaagttaa ttttcctaca aaagttgttg gaattacaga aaaaggagaa
      781 cttgatttat gtcaattaga gaatatactt aaaaattatg ctggtaatgt atctctcgtt
      841 acagttacag gagctagtaa tgtcacaggt ataaccactc ctatatatga aatagcaaga
      901 atagctcata agtatggtgc aaagatattg gtggatattg tgcaagtaat tcagcataaa
      961 cctttttcaa tgatgccaca tgaatgtgat gagcatatag attttgttgt ttttagtgct
     1021 cataaatgtt attctccact aaatggagga gctctggtag gacctaaaaa attctttgaa
     1081 aaatttaatc ctgtactata tggctcaggt tctaccaaat ttgtaaatga taacaaaata
     1141 ctatatgcaa acgtacccca acgttttgaa gcaggatacc cagagtattt tggaactctt
     1201 gcaatgggaa aagcactgaa cactttaaat aaaattggtc taaacagaat ttcaaaatac
     1261 gaaagagaat tatttttata tgctaaagaa gaaatgagta aaatacctaa tataataata
     1321 tatggagata aatcaaataa tgtaattatc ccaaatatat cttttaatat aagtaatatg
     1381 tattacaaag atgtagcaaa atatttagtt gataactttg gaatagaaac tggagctgga
     1441 gtagtaggtg cagatattta tgtacaaaaa ttattaggta taagtccaaa agaagcatat
     1501 gtcagattta tgagggaaaa tcctgtaggt cttgttagga taagcttagg aatgtacaat
     1561 acttttgatg aggtggatag ctttatacat gctattaaaa ctttggcttt taaataagtt
     1621 ttttctattc ttttttatca ataaatagag agtagttttg ttttcacaaa aaagcaaatt
     1681 atatcaacag tcaatttcat tagcaattag acactcattt gtaactatac aaactatttt
     1741 acactataca aacaaaaaac tcattacaat gagttttttt gtttatatat gagatttatt
     1801 ttaattactt caatttactt taattacata tatgattcaa ttttgttttt taaattctcc
     1861 ttaggcataa aacctattaa tctatctaca ggttttccat ccttaaatat tagcatagtt
     1921 ggtactgtag atacttcaaa tttctgagct aattctatac tttggtctat gtccaccttc
     1981 aagaagtttg ctttttcaac catctcattt cctaaagctt catatattgg tgatagcatc
     2041 ttacaaggtc cacaccaagt cgcaaagaaa tctacaacta ctaaaccatc tttattttct
     2101 acttcattta taaattcatt attatttact atttttacca taatatacat ctcctttatt
     2161 atcttcttga tattaatata ctacgaccta tataacagtt caattaggca cttttttgta
     2221 accgcctatt tttaatagat ttaatattat gataaataga tttataagat taaatattta
     2281 taagactaaa tatttctaat atattttatt tttttcttaa tatagttgac attttttccc
     2341 ttaaattata aaatcaaatt aataactaca taggtagtta ctaatctttt atcaaaggag
     2401 tttctatatg gataatatta ttaatgaatt acaaaatata ggatttacta aatacgaatc
     2461 tcaaatatac atatcattat taaaggaaag ccctttaaca ggatatgaaa taagtaaatt
     2521 atcaggtgta ccacaatcaa aagtctatga aaatataaca aagctattaa acaataatat
     2581 aattatcaat gttggaactg accctattaa gtacatacca ataaacccta atgaattatt
     2641 aaaaaataag aaaaaagaat ttgacaattc tatggataat ctaaagaata gcttacagat
     2701 attaaataaa agtaaaagtg tagaatacgt attaaacata aaaggagata aaaatatatt
     2761 aaaaaaagca atagaaatga taaaaaatgc aaaacaagaa attataattt cttgccctta
     2821 tgaagaaatt cttgaattaa aaaaagaact cctaattgct tacaaaaaaa atattaatat
     2881 tgaaatacta cttttagaaa acaaaagtct gaaaggtctt gataatattc atattcatgg
     2941 aggtaataaa actacacttg aaaataaaat gctttacaaa ggaattattt tgattgtaga
     3001 taatgatgaa atattgacag gaaacttatc agaaggcagt gaggccattt caatttggac
     3061 aaaaaactct aacattttat atattggagt tcaatacata aaacatgaaa tttatatttc
     3121 agaacaaata aaaggaggtt ataaaaatga acaaaattaa gattaaacaa gttgatgctt
     3181 ttacaactat accttttggt ggaaatcctg ctggtgtcgt tactgatacc tctaacattt
     3241 ctgatgaaat taagcagaat gtggctaaag aaatgaattt atcagaaact gcatttgttt
     3301 cagagtcaaa tattgcagat tttaaagtac aatttttcac accaggttta gaagttgatt
     3361 tatgtggtca tgcaacaata ggtactttcc atgcactttt ggaagaaggg aagcttaata
     3421 ctaataaaag tatattttat caagaaacaa aagctggtgt attatctgta gagcttaaga
     3481 aaataaataa tgaaaatata tttatgatgg aacagaatac acctgaattt gaaaatttag
     3541 ataattgcag aaaagaactt gcacaaatga taggtgtgca tgaagataga cttcttgaat
     3601 atcctattat aaaggtaaat actggacttt ggtggcttgt atttggtcta aaatctttag
     3661 atgatttaaa aaatatctct ccagatttaa ataaaatcaa aaaatttagt ggtgataata
     3721 atataattgg tataactcca ttttgtatag aaactataga taaaaagtgt gactaccatt
     3781 taagatctat agcaccatat gttggagtta cagaagaccc tgtatgtgga actgggaacg
     3841 cttgcgtatc atcttatata gtacatcaca atattttaga taaaactagt tttattggtg
     3901 agcaaggtaa ctttataaac agacctggaa aggtatatgt aaatataact agagaaaaca
     3961 ataacataac ttctgtaaaa attggaggaa gtgcttatac tgtcttaagt ggtgaaatta
     4021 tgtattaatc ccatacctaa taaaaacaaa taaaaatact agctaactaa agctagtatt
     4081 tttatttgtt atttttattt aataatcata ctctaacaaa tattataatt atatatatca
     4141 taatattgta tttagaagta aaattttatt tttattatta attagttata aatcaatcta
     4201 atacaacata attacagtag attaacctat aaaacataat aattgcttac tgattattta
     4261 tcgtcctcaa caagtctact taatgtctta tcagataaga ataataatat cgctgcaaca
     4321 aatagtatta ctggaattat agtatatact ttaatcatat caaatttaag agtaaatgca
     4381 tatatatatc catatccttt accagctata aaagtagcaa atgtccatac tcccataaga
     4441 acacctagaa gcttcttagg tgcatactta cttacaaaag agttaccaag tggtgagaat
     4501 aacatttcac caagacttaa aagaatacca aacatgatta tccatagtat actagcttta
     4561 gcagtttcag gagctccaac tcctcttgaa aattctgcac caacaagcat taagaatgca
     4621 agacctaaga atattaatcc aagacctgtc tttttaaata aactcatatc tccttgtggt
     4681 ctactagcaa gtttaaacca taatgctcca agaactggtc ctaatactat acatacaagt
     4741 gaatttaatg agtcaaacca agcaagagga acatcaaatc ctccaacatt catgtttaca
     4801 aatgctgcac cataatcata aactgcaaga taagttagat accagaatac ccagaaaatt
     4861 acagagaaga atgatataag tataatagcc caaattctct tcttatccat gcttgtaaga
     4921 ggagacttat ctttttcttc aattttttca gcaactacac cttctttaaa aggtctcttt
     4981 cctgcattcc caaggaatct ccatccatat atgaaccata ttgcacctat tacacaaagt
     5041 acagctgcta taaagaaaca ttgagaaaat ccaagtacac cattttttgc aaatgtttta
     5101 aggtaaagaa taccaactgc tatagtacca ataaaagaac ctatattaac aaaagaatat
     5161 tgtactgaaa atgctgtatc aagttcttct tgactatcaa aaagctgtcc atttaccgca
     5221 gatacgttac ctttaaagaa tgcagtacca attgaaacta agattatcat tgcatttacc
     5281 atagaaactg tagttgcaat agaaccaaga tagtatccta cccccatgat aagcatacct
     5341 ataggtatag tatatctagc tcctatccat ctatctgata tatacccccc aattataggt
     5401 gcaaggtatg tgaatgcaac aaggttggct tgcattatag cagcttgtcc tttatctatt
     5461 ccaagaccac catgaactat agctgttgta aggaatatat atattaagta ctttgaagca
     5521 taatatgctg ctctttcaaa tgagaaagtt gttgagcaga tataaaatcc cataggaaat
     5581 ttacccttct tttttactgt tgagctcata attttaaatc ctttcttaat tgtattttta
     5641 ttttttacgt tttgtccaaa ttctttcttt tatgaaagat ataatattat tattttgtaa
     5701 aacaatttgt aattattatt aagataattg cgcatttatt tgtagagtac atattctcat
     5761 ggaataaaat taatacactt cttaatcata aatttaaatt atataaattc actattttca
     5821 gatatagtta taaattaata tattctcatc taataccatg aaaaaacctt cgccatatgc
     5881 aattcttatt ttttacgtga tagtatttta caaactaaaa tccccctttt gtagcaactt
     5941 atgtaattac attttagtgc ttttttaaag gagtctcgaa taaagcaaca tatattttgt
     6001 tcaaataatg tttttattta ttttttaaat ttatatatta cttcacccaa ccaattctca
     6061 acttaaataa ctaaactact atctattcac agattaggct tatttcttac acattagttt
     6121 ataataaaca ctgtgaatct aaacataaaa actgttattt taaagtaatt acccatatat
     6181 tcgctttgat actatttatt gaggctatac tctttatgta taatatatta tttataaaat
     6241 acctcaaaat atattattca taaaatacct caaactgaaa actttttcac gaatatttat
     6301 ttttcacctc ccctttttta tttcaccatg taaatcgtac cataaatatg cacttatatg
     6361 caagaaaaaa atctattaat cgacagtaaa taattagaaa aattcaaatt atattt
//
LOCUS       DAMJZE010000198         6370 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJZE010000198 DAMJZE010000000
VERSION     DAMJZE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.202"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            295..1239
                     /locus_tag="PC541_003375"
     CDS             295..1239
                     /locus_tag="PC541_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2840796.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1261..1887
                     /locus_tag="PC541_003376"
     CDS             1261..1887
                     /locus_tag="PC541_003376"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2840797.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1921..2970
                     /gene="hisC"
                     /locus_tag="PC541_003377"
     CDS             1921..2970
                     /gene="hisC"
                     /locus_tag="PC541_003377"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2840798.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2973..3551
                     /gene="hisB"
                     /locus_tag="PC541_003378"
     CDS             2973..3551
                     /gene="hisB"
                     /locus_tag="PC541_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2840799.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3553..4158
                     /gene="hisH"
                     /locus_tag="PC541_003379"
     CDS             3553..4158
                     /gene="hisH"
                     /locus_tag="PC541_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2840800.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4155..4877
                     /gene="hisA"
                     /locus_tag="PC541_003380"
     CDS             4155..4877
                     /gene="hisA"
                     /locus_tag="PC541_003380"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2840801.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4859..5617
                     /gene="hisF"
                     /locus_tag="PC541_003381"
     CDS             4859..5617
                     /gene="hisF"
                     /locus_tag="PC541_003381"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2840802.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5645..6313
                     /locus_tag="PC541_003382"
     CDS             5645..6313
                     /locus_tag="PC541_003382"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2840803.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2604 a          538 c         1260 g         1968 t
ORIGIN      
        1 ctgtaaattt agaatattaa taaaaattat ttgggaggaa attatcatgg tgagattaaa
       61 tatagtaaaa ttaaatacaa aattaaatca tcaccatcat catagattaa agggattgta
      121 atatgaaatt aattcataga cacaagccct gtttttagtg taattggtct tgtatctatg
      181 atgggaagta attttaagag aaaattatag ccatataggt ataaagccta tgtggctttt
      241 ttattgtttt aaatttgcaa atataagagt aaatttaatg aagagggtga gaatgtgaaa
      301 tttttgagga ttgaagatga ggttatttat tctaaaaaaa gatataaatt aaaaagggaa
      361 atagataaac tttttttgga tgaagaatat tttcaaatag aaccacaatt atttgaagaa
      421 tatgatgaat ttacaactat aaataataaa attccagaag aatctatggt aaaggttgta
      481 aatggaaaag taatggtact tagagctgat ataactacaa gtataataaa aagccttgta
      541 ccaagatggg aagatggttt aaagcttaag cttttttata attcatcaat atataaaaat
      601 aaaaatacag ttggaataaa agagataaga caaataggtt gtgagtatct aggagaagcc
      661 tctgtagaag cagatagaga agtagtgaaa ttagctctta agatacttga aaaatacaat
      721 aataatttta ttctagaggt tgggagtagc aagtatattc atggtttgtt agaagaatta
      781 aatttaaata agaattgtga aaatcaaatt aaaaatttac tatatacgaa gaacactcat
      841 gaactaaaag tatatattga agaattaaaa attaagcgtg aagtaaaaga gttattatca
      901 aatattttaa gtttacaagg gaatttatat aatgttattg aaaaatcaaa caagttctat
      961 tgtaataata agatgaagca agctcttgaa gaattaaagc aagtaaataa tttaattgag
     1021 gaatgtaatt ttttagataa ggctagattt gatttatcaa tgattactat gcttgactat
     1081 tatgaaggaa ttatgtttag aggatactac ccaaactcat ataaagaaat tcttagtggc
     1141 ggaagatatg attctttaac taaagagtat ggaaaagaaa ttccagccat tggatttaca
     1201 ctaagtgttg atgagttaat gaagtatgta tataagtaaa gtttagaagg agggattgct
     1261 ttggattatt tgactatagc actagcaaaa ggacgaattg aaggggaatc atttaaaaaa
     1321 tttaaaaaaa tgggtctagg agacagtata gacacagata cgagaaaact aatttttaaa
     1381 gatgaagaaa ataaaattat atatatacat gtaaagccat cagatgtagt tacatatgtt
     1441 gaaaaaggag tcgctgacct cggtatagca ggtaaagata ctattttaga aaatgagaca
     1501 gatgtatatg aaatatatga tttaggtttt ggaaaatgta aatttgctgt agcaggatta
     1561 aagggagata gcatatatag agaagatgaa tatttaaaag tggcaacaaa gtatcccaat
     1621 atagcaaaaa agtattttaa agagaaaggt caaaaaattg aaataataaa attaaatggt
     1681 tctgtagaac tagctccaat agtagggttg tcagatgtaa ttgtagatat agttgagact
     1741 ggcaatacac ttaaagcaaa tggtcttgaa atattagaag acatatgtaa tataagtgct
     1801 agaattatat caaatagagc tagctataga tttaaatatg aacaaattca gaatataatt
     1861 cgtttatttg aagaacttga taattaaaat ataattgagc tttataagga gggatttaag
     1921 ttgagagaaa aagaaagcat aagagagtta aggggatatg aaccaaatca cgtaaactgt
     1981 aaagtaaaac ttgatgcaaa tgaaggaagt aagaggttgt ttaaatacct tattaaagaa
     2041 atttcagata gtgatataga tttaaattta tatcctgaag attcttatag tgatttgaaa
     2101 gaatctataa tagattacat aaatatttct ggagtaaata agaaaaatct tctagtagga
     2161 aacggttcaa gtgaaataat tgatttaata atacatactt ttgtagataa agatgaagta
     2221 atattgagtt tctctccaag ttttagtatg tattctatat atagtcagat aaatgggtct
     2281 aaatttatag gtgttgaaag tgatgaaaat ttagttataa atatagataa tgtgattgaa
     2341 aaagtaaagg agaataatcc taaaatagta attgtatgta atcctaataa tccaactgga
     2401 acaatattaa agagggaaga gataataaag cttttagatt caactaatag tctagtggtt
     2461 ttagatgaag catatatgga ttttggagaa gaaagcatgt taagtgatgt ttttaagtat
     2521 gataatctaa tagttcttag aactttatca aaggcatttg gattggctgg aataagaaca
     2581 ggatatatgt tatctaatag tagtttaata aattctgttg agaaagtaag acctccatat
     2641 aatttaaatt cattatcaga ctttattgct acaagagcac ttagaaataa agatgtggta
     2701 aaggcttata taaaggaagt aaaagaggaa agagaagttt tatatgaaga aatgattggt
     2761 atgggaatta aagcatacaa atcacaagca aattttatat tattttattc tgaaatcgaa
     2821 aatttatcac aaaaacttat tgatagaggt gttttaataa gaaaatttgg tggaaagtta
     2881 gaaaattatt acagagttac aattggtgat aaagaagaaa attctatgtt tgttggagct
     2941 ataagagata ttttgaaaaa ggagaaatag gtatgagaat ttggaaggta gaaagaaata
     3001 ctcttgaaac acagatttta gtagaattaa atattgatgg ttctggtaaa gctgaaatag
     3061 atactggaat aggattttta gaccatatgc ttaccttaat gtcatttcat ggcaagttcg
     3121 atttaaaagt gatttgtaag ggtgatacat atgtagatga ccaccatagt gtagaagata
     3181 taggtatagc tataggtgaa gcatttaaaa atgcactagg tgacaaaaaa ggaatacgaa
     3241 gatattctaa tatctatata cctatggatg aatctctatc aatggtagca atagatataa
     3301 gcaatagacc ttatcttgta tttaatgcta aatttgatac ccaaatgatt ggtagtatga
     3361 gtactcaatg ctttaaagaa ttttttagag cttttgtaaa tgaatcgaga gtaacattac
     3421 atatcaatct cttatatggg gaaaatgacc atcataagat agaaagtatt tttaaagcat
     3481 ttgcaagagc tttaaaagag ggtagtgaaa tagtttctaa tgagatagca tcttcaaagg
     3541 gggttttgta gaatgaatat aatagtagac tatggtcttg gaaatataga ttctgtgtct
     3601 aggggtttta gaaaagctgg aatagaaact aaaatttcta gtgatataga tgaaataaaa
     3661 caggcagatt cacttatact tccaggtgtt ggagctttta gagattctat aagtgcctta
     3721 gataaactag gattgatacc aataataaag gagcatgttt ctaagggtaa atttatgata
     3781 ggtatatgtc taggaatgca attactttat gaaaaaagtt atgagtatgg agaatatgaa
     3841 ggattaggac ttataaaagg aagtattgat aaactggata taagtttaaa agtgccacat
     3901 atgggatgga ataatctaaa atttaataag gcaaatgatg atatacttaa atatataaat
     3961 gaagatgatt atgtatactt cgttcactca tattatgcaa attcatcaaa tgaagaacta
     4021 attgcatttt cagaatatga aaagaaaatc cctgctattg ttagaaaagg caatgtatat
     4081 ggtatacaat ttcatcctga aaaaagtgga gaagtagggt taaatatatt aagagcatat
     4141 ggggagatga taaaatgata atatttccag caatagatat aaaagataat aagtgtgtaa
     4201 gactcacaca aggagaattt gacaaagtaa atgtatatta tgataatcca ttagaagtag
     4261 catataaatg gaaaaatgaa ggagcagaat atatacatat agtagattta aatggtgcta
     4321 gaagtgaatt tggtgtaaat acaaaaataa ttgaggacat agcaaacaat atagatatac
     4381 ctattcaagt tggcggtgga gttagggata aggaaaaagt caaaagttta ataaatgctg
     4441 gtgttactag agtaatatta ggtagtatag cgattgaaaa tttgaattta gttgaagaat
     4501 tggtcaatga atataaagag aaaattgtag tttctataga tgctaaagat ggaaaagtag
     4561 cagttagggg atgggaagtt gtaagcaatg tagactcatt aacattatgt aaacaacttg
     4621 aaaagatagg tgtacaaact attgtttata cagatatatc aaaagatggg atgttgcaag
     4681 gtccaaattt tgacatatat gaaagaatag caaaggaaac atcactaaat gttattgctt
     4741 ctggaggcgt aacttctata gaagacgtaa aaagactaaa agctatgaac ttatatggtg
     4801 caataatagg taaagcgctt tatgataaaa agatagactt taaggaggca caacaattat
     4861 gcttactagg agaataatac cttgtctaga tgtgagaaat ggaagagttg taaaggggaa
     4921 aaaatttaaa gatatagtag atgttgatag tccagaagta cttggaaaat tttatagtga
     4981 ttgtggtgca gatgaacttg tattctatga tataactgca tcaaatgaag agagaaagac
     5041 atctttagaa tttgtgacaa aagttgcaga aaatataaat ataccatttt gtgttggtgg
     5101 aggagtaaat aaattagaag atttcactga tattttaaga aaaggtgctg ataaagtttc
     5161 tataaattca tcagcagtca aaaatcctga attaataagg gaagcttcat taaaatttgg
     5221 agcacaatgt gttgttttat cgatagatgc aaagaaaaat gaagagggtt catggagcgt
     5281 atacgtaaaa ggtggaagag aaaaaactaa tctggatgca attgaatggg ctgttaaagg
     5341 tgtagaactt ggagcaggag aaatcgttgt aaatagtatg gatgaagatg gaatgaaaaa
     5401 tggatatgat atagaattat tatcaaagat aacttcttta gtaaatgtac ctgttatagc
     5461 atctggaggc gctggaaaaa aggaagattt ttatgaggcg gttaataaat ccaatgtaga
     5521 tggaatttta gcagcatctg tttttcattt tggcgagata aaaataaacg atttaaagaa
     5581 atatttaaaa gacatgggtg tagaagtaag attataagtt aaatactaaa aggagattag
     5641 aaatatggat aataagtgta acaatgtata cagtgatgaa gtagagaaat ttataagaag
     5701 tataaagttt gatgataaag ggttagtccc tgttgttgtt caagaggtag ttagcaaaga
     5761 tgtactgatg ttagcatata tgaataaaga tgtgataaag aaaaccttaa aagataaagt
     5821 agcttgctat tttagtagaa gtagacaaga actttgggtt aaaggtgaga catctggtaa
     5881 tactcaaaaa gtagttaaga tgtcttatga ttgtgatgta gatactatac tactttttgt
     5941 tgaacaaact ggtgtagctt gtcatacagg caattattct tgtttttaca gagacttgtt
     6001 tgatgatagt tcaaaaatgg agcttgaagt acaaacaaat atattgaaag aattatatga
     6061 tttaatcaat gaaagaaaaa ataatccagt tgaaggttct tatactaatt atctttttga
     6121 aaaaggaata gataagattc ttaaaaaagt aggtgaagag agtagtgagg taattatagc
     6181 atcaaaaaat actgataaaa gtgaacttat ttacgaaatt agcgatttag tatatcatac
     6241 attggtttta atgatagaaa aaggtgttga aattgatgaa ataaaaaaag aattacttaa
     6301 aagaagaaaa tagaataaga tagattaatg ggtaataaga aaatatacca cttttaataa
     6361 aaagtggtat
//
LOCUS       DAMJZE010000199         6363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJZE010000199 DAMJZE010000000
VERSION     DAMJZE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.203"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            143..3322
                     /locus_tag="PC541_003383"
     CDS             143..3322
                     /locus_tag="PC541_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2840804.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3607..4371)
                     /locus_tag="PC541_003384"
     CDS             complement(3607..4371)
                     /locus_tag="PC541_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2840805.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4527..4760)
                     /locus_tag="PC541_003385"
     CDS             complement(4527..4760)
                     /locus_tag="PC541_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840806.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4834..6327)
                     /locus_tag="PC541_003386"
     CDS             complement(4834..6327)
                     /locus_tag="PC541_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2840807.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2362 a          907 c          740 g         2354 t
ORIGIN      
        1 aaatctaata gtaaattgtt atttacacat atatcgtgat agataaaagc tattcatatc
       61 tgccttaatt ttagtcttaa atttagagtg taaattgaat gcatttagat tataatatat
      121 acagtgaggt aaggtgaata atatgaaaat agatatgata tataaactaa taagaagaaa
      181 tacagaacca tcaagatggt caagaggtgt aaaatattat actaatcaat tagttgatta
      241 tatagacata gatattgatg acctacaaaa taatctgttg acatttcgtg gtggagtcaa
      301 ttctgaagat aattatagct tttattttaa ttcaataact atagatataa agcactttaa
      361 gataattgaa aatgattgta gttgcgctga ctgtcaatca aagggcaaaa gtatttttat
      421 atgtaagcat atagttgcta ttgtgataaa aagcttaaaa caaataaaaa ataaaaaaga
      481 tgttcctcct attctaatag atatattagg tgcagataag gataagcttt ctaatacaaa
      541 ttctaaaacc agcttagatt taagcaagca attactttct ttgttagaca atactaaaag
      601 agaaagggtt aatttagatg tattgataaa tgtggattca aataaagcaa ttgctgaatt
      661 taaaatagga attaataaaa tgtacatttt aaaaaacatt actgaatttg catattcaaa
      721 acttaatgga tatgaattag attttgggaa gaaatttact tacaacccca acactcatta
      781 ttttgctgat gaagatgaga agttagtaga tattatagaa gaatatggga caaatctttc
      841 atacaacttt tcttcatcac attcaaaaaa atttatggag ataaatgcaa gctctttaaa
      901 acaattttta aatgctatta agtacaagga ttttaccctt attttttcta aagaggaata
      961 tcatccaaag atattaaatg atactctacc tgtaaggttt gattttaaaa atatagaaga
     1021 taaaatagtc ttatattcta aagattcact acctgttcca cttactaaaa aaaatgatgt
     1081 acttttatat gatggcaata tctatttatt atcacataaa aaagctcatg attattcaaa
     1141 aatatatcaa atattatcaa agactaaaga gttaaaattt gataaagagg attctaagga
     1201 tgttttagga ttgctagttc ctagattaaa atctatatct caagaagttc atttagatga
     1261 taacataaaa aataatataa caaaagattt taaatctgag ttctactttg atatgattga
     1321 tggcaacata tgttgtgatg taaagtatat atatgatgat gaagataaaa aatttgtgct
     1381 ccccaacata caaaaggaag ctactattga aaataaactt acttcttcac aatttacaaa
     1441 ggaaaataat cattatgtgt ttaaaggaac tgaccatgac ctatttacat ttttagattt
     1501 acaattagaa tctctaaagg aatttggaga tatatattat tctgagaagt ttaaattgaa
     1561 aaaaatatat aacgcttcat caattcaagc ttcaatcaat cagactaacc agaattacct
     1621 agaatttaat tttaatatat ctgatataaa tccaaaagag tacaaggata ttttgaaagc
     1681 ttttcaagaa aaacgtactt tttataaact taaagatgga aactttatag atttatcaga
     1741 aagagaaaca aaagattttt ttgaattagt tgaaaatcta gatataatgg ataaatcaaa
     1801 aaataataaa attcataaaa ataaagctct atatataaat gatgtaatac gtagtaaaaa
     1861 tttaaagttt atagatggta aaaaagaact agacagtata tgtgaaaaat ttagaaattt
     1921 tgatagcata aatttagaaa ttcccgcaaa tttaaatgca aaccttcgtg actatcaaat
     1981 aaatggcctc aattggttta aagttttaga ttattataaa tttggtggta tattagctga
     2041 tgaaatgggt cttggtaaaa caatacagac tattgcattt ttattatcat tatctaataa
     2101 aaaaagtctt attgttacac cgacatcact catttataat tggaaaaatg aatttgaaaa
     2161 gtttgctcca gatataaaag ttttattgat acatggaaat aaaagagata gagaaaaatg
     2221 ttttatggaa ttagaaaatt ttgatgtaat acttacaaca tatggtactc ttagaaatga
     2281 tttagacaaa tatagtgaaa taaagtttga ttattgtata ttagacgaag ctcaaaatat
     2341 aaaaaatcca gtggctttag taactgagtc tgtaaaaagt ataaatgcag aaaataaatt
     2401 tgcactcact ggtacaccta tggaaaataa tctactagaa ttatggtcta tatttgattt
     2461 tataatgcct ggctatctat atagtaaagc aaagtttcag gaattgttta taaataaaga
     2521 agataatgtt aaaaacctga aaaaattgat aaaacctttt attcttagaa gaagtaaaaa
     2581 acaagttatg aaagaacttc cagataagat tgaaaaaaac ttttttgttg aattgaataa
     2641 agagcagaaa aagatatata gtgtatattc aaaagatata caagataaaa tgaaagataa
     2701 aaatctaaag aaagataaaa tagttatatt ctcttattta actaaactaa gacaactttg
     2761 tttagaccca agtatagtag taaaagatta caataaaaaa agttcaaaaa ttgaaacatg
     2821 tttagaaata cttagggata gtataaacga aaaccacaaa atattattgt tttcacaatt
     2881 tacttctgtg cttaagaaca taagtaagga acttgataag tataaaatta aataccatta
     2941 tatagatggt aaaactaatg ccaaagaaag acttgaactt gtagatgagt tcaataatag
     3001 tatggataaa aaagtttttt taatatcttt aaaagcaggc ggaactggtt taaatcttac
     3061 atctgctgac atggtaattc actttgaccc atggtggaat ccatcagtag aaaatcaagc
     3121 tagtgataga gctcatagat ttggtcaaaa aaattcagta caagttataa aattgattgc
     3181 taaagggact atagaagaaa aaataataaa attacaagaa agtaaaaaag agcttataaa
     3241 tcaatttata aatggagaac tttctaatga aggtgtatta aaaagtctat ctgatgagga
     3301 gataataaac ttatttaact aaattattta atcctacagc ttaactatat gaatttgttc
     3361 taacatttat atagttaagc taaaatatct gaataaataa aatttaatat tgttatttaa
     3421 tcccaacttt acaatctaat gatatctctt tctatacata ttgcatatac aaatgtaata
     3481 acttgactat tagaatattt tgttttaaca atcctatttc atcataataa tgaagtatat
     3541 gtacactcac ttttgtaagt tttgcaatcc attttacagt tttcattatt gtaaattttg
     3601 gttaaattat tcctctaaaa atttctttgc tacatttgaa aaatctaatc cattcgataa
     3661 aattgcagga tgaccacctt tatcaaacaa atacatttta ccatttttaa tcttttcaag
     3721 aagtaatgaa aaagtatttt caaaaaaatc actagatatt agagatacaa actcatcttc
     3781 tctacttcct gttaaaagca cctctggttg catagtttct agaggcttat gaaaaaactt
     3841 tcctatagtt ttataatgtt caaaaatagc ttctgtatca ttatctacaa ctttttccca
     3901 gttttctcct tgattataaa tataaaaagc ttttgcacca tcatcttgct tagatgcttc
     3961 acgctctatt ttcacatttt gtacaaatga ctctagagga acttctcctt caaaactatc
     4021 tgcaattacc ttattcacta aatcaggtct ctcaagtgca acatttaatg caacaagagc
     4081 tcctccacta ctaccaatta tgtttacttt tttatagttc attgcttcta aaaaacatat
     4141 cacttgcata gcctcatcaa accataagtc agcagaaaat ttatcgacca tttgagattt
     4201 tccatgacct acaaaatcaa ttaatattac tttatattca tctttataaa aattgattat
     4261 atcattaaac atcttggaag aggctgtatt tccatgtaaa aaaagtaatg gtgtaccatc
     4321 cccaatttca ttatagtagc actgtttgtc gttatagtta aaatatgcca tatatttccc
     4381 ccctttaata tccctatagg tatatattta ttacttatat ttaaaatgac tattccctta
     4441 gccacttaaa aatagtataa cttagaatgt agataaaatc aacattcctt gctaatttat
     4501 ctattgtttg tctaatttat tataatttaa cttctgtaaa atttcttttt tgtacgaaaa
     4561 tattccttcc atttacaaca atcattcaaa tgaagtataa catgacgagt aggaggcata
     4621 agcaaaattc cagcaatatc tttattcccc caaaagtcca acaaccaaat agaatcttca
     4681 tgctttatta ctcctccagt caccaaaatc ctttctaagt caattggaga gacctttcgt
     4741 ttcatatctt cagctttcaa atccttaaca acttctattg tgttttttgc aacttcattt
     4801 cgataacaaa gcaattcatc aatattgact aaattatcgt atatctcttt tcactttaac
     4861 catttttttc tttataggta tctccttata tttttttaat atcaaatctc ccttataatt
     4921 taatatatct acatatgaac ataaatcttg aacttcaatt acacctatat catcattagt
     4981 tatacctttt atattactaa atgcaccaac tatatctaaa actcttattt tcttcttctt
     5041 accagcattt aaatataatt taacaacttc actttctata tttagtttta tatctttact
     5101 tttgtaagaa tttttatcaa ctttttttct ctttgatact ttttttagga ttccttttga
     5161 atattcttca aacaaaactt tgccatgtgt aattctactt ttttctatat cttttaattc
     5221 atttatttta tatcctatat acgtttctat attctctata tatttttcat ccttttcagt
     5281 tactatagta attgcctttc cactatttcc ttttctccca gttctaccta ttctatgtat
     5341 atagttttct ttatcttgtg gcacatcata atttattact aaagaaatat catcaatatg
     5401 tattcctctg gacgctacat cactactaac taatatatta aatttttggt ttttaaaatc
     5461 cttaattaca aaaattcttc tttcttgtga taaatctgca tgtaactctc ttattaaaaa
     5521 accctcttta ctcatcttgt tatataattt agatactttc tctttggtat tacaaaatat
     5581 tatcacactc tttggatttt cgctgtatat taattctttt aaaaggatgt acttctctct
     5641 tccatttgcc ttgattattt tgtcatctat ttgtcttgtt ttttgattag tatcgctctc
     5701 attttcttct atatttataa cactgtaatc tagcatatat ttttcacata tatattttat
     5761 ctcttcatct attgtagctg aaaaaagact tactattttc tccttaggta aatttaataa
     5821 tattttttcc atttgttcaa caaaaccttt attaaacatt ttatctgcct catctattac
     5881 taaatacttt acattttcca acttaataga acctctattt atatggtcaa gtattctgcc
     5941 tggagtagct acaacaatat gaaccctttg tttcaattca gctatttggt cttttataga
     6001 ttgtttgcca aatatagcac tacatctcac cttctttagt ctccctatat ctgatatctc
     6061 atcttttact tgcaaggcta attctcttgt tggaaccact ataagagctt gaatattatt
     6121 gtaatctaca tttatattct cgcataaagg tattgcaaaa cttgctgtct ttccacttcc
     6181 tgtttttgac ctaactacta agttttgacc ttttaataat tttggaataa cttctctttg
     6241 tactctagaa ggtatattat atcctaaact cttcaatgac ttaagtatct tttcatttaa
     6301 cttaaatttt tcaaatgtat atttcatata ttaccacctt attatattta tcttatctat
     6361 ata
//
LOCUS       DAMJZE010000200         6316 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJZE010000200 DAMJZE010000000
VERSION     DAMJZE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.204"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            62..700
                     /locus_tag="PC541_003387"
     CDS             62..700
                     /locus_tag="PC541_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2840808.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            832..1494
                     /locus_tag="PC541_003388"
     CDS             832..1494
                     /locus_tag="PC541_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840809.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            1494..2675
                     /locus_tag="PC541_003389"
     CDS             1494..2675
                     /locus_tag="PC541_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2840810.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(2856..5516)
                     /locus_tag="PC541_003390"
     CDS             complement(2856..5516)
                     /locus_tag="PC541_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840811.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5513..6196)
                     /locus_tag="PC541_003391"
     CDS             complement(5513..6196)
                     /locus_tag="PC541_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840812.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         2212 a          972 c          873 g         2259 t
ORIGIN      
        1 attatattta cataaatata ccatcagtat acttttttat caatagaaag gaataattta
       61 tatgtcagaa ttatcaaaaa gagataaaga aaaaatacaa agagaaaatg agattattga
      121 taaggcagaa aaattatttt gtcttaatgg atttgataat acaactatga atgaattagc
      181 aaaagaagtt gaatatacta aacgcaccat atacaagtac ttttcatgta aagaagattt
      241 attctttgct gttgtcttaa gaggatataa aagactttgg gataatgtta aaatagaaag
      301 tgcaaaaggc aaaacagggt ttgaaaaaat aaaactttca tactttgctt ttcataaatt
      361 ttactctgta gaaccttctc tattatgcct tatgggaatg attggtattg taaactctga
      421 aaaatcggat actgatatgc tgtttaaaga aaagtttttt tcttttaata aatttatgtt
      481 tgatgaaata caaggaatgt ttgaaattgg aaaaaatgat agaagtatta ggcatgatat
      541 tgaaatacct actcttatgt attcatcaat atttacactt acaggatttt ttaatttact
      601 atctgtaaca ggaaaatcat acctgaacaa ctttaatata gatgaggaaa aatttattga
      661 aacaactctt gctcttttaa tagattcaat taaagcataa acctaagttt ataaattaaa
      721 gtttatatac ttaaatgata attttttcta tctctaatat gaaaaatatt aaacataaaa
      781 ctaataactt agttaatgga ataactaaat tactgaaagg attaaaagta aatggctaaa
      841 atacttcttt tagaagataa tgatacaatt acatttggaa taaaaacttc ccttgaaaag
      901 aaaaatcaca ctgtatgttg ttataatacg attgctgaag ctaaaaaaaa ttttgataaa
      961 actattgatt taattctgct tgatttaaat ctacctgatg gtattggata cgagttttgc
     1021 caatatgtta aaagtataga agatactcca gtgatatttt tgacagtccg tgatgatgaa
     1081 aaagacatta tacgtggatt agatatgggt gcagatgact atgtggttaa accgtttttg
     1141 ctctcagtac ttcaatccag aattaatgca gttcttagaa gaactcattc aaatactaac
     1201 aatactatta catgtgaaaa tataagcttg aataaggttg aagtcaaagc atatttagat
     1261 aatgttgaag tacctttgac agcaggagaa tataaacttc ttctaagtct tatggaaaat
     1321 aaaaatagag ctatgactcg tacaaagtta ctagaaatgc tctgggatgt agatggtaat
     1381 ttcgtaaatg ataacactct tactgttaca ataaaacgtc ttagagaaaa attaaaaaat
     1441 ccaccttgta taaaaacatt aagaggcata ggatatagaa tggaggacaa ctagtgaaaa
     1501 ctccaagaaa tatcaacagt acagttgttt ttataatatt ctgcttaatt gtaacttttt
     1561 tattgagtgt aattctaaca actataactt cttttaatga atacaatgta ctgtctaagg
     1621 ctcttggtgc atcatatgag tacaatccta agtctacaga aagtatacta aaatcactga
     1681 aaaattctag tgataaggat tttaaaacag gaaatgaaat tttatcaaaa catggatatt
     1741 ctaaaagtac attttggagt aaaaactttt tttattttct agtgatatcg tttggtataa
     1801 tttcaacaat ggctacagtt gtatatataa tcaaacatag aacaaataag caaaaactga
     1861 gtagaataaa caatcttact caatacttag aagaagtgaa tttaggaaaa gagtctgtac
     1921 ttttacgatg tgaagatgaa ttttctcacc ttgaagatga gatatataaa acagttgggg
     1981 aacttcgttg ttcaaaagaa tttgctttaa aagaaagaca aactctttca gataaccttg
     2041 cagatattgc tcatcaactt aagactccaa ttacctctat gtcacttatg gcacaattac
     2101 tttcagaaaa ttgtccagat gaagaaatag attatattaa cagacttaca aatcaaatct
     2161 caagacttga gagactagtt tcatctttac ttacattatc aaaattggat gcaagtactt
     2221 taatatttga aagcaaactt atagatgtac attctcttct tacttatgca attgagccta
     2281 tagaaggttc tttaagagaa aaacatcaga catttactat cgaggcttct ccttcggtag
     2341 aatttgaaat cgatattaat tggactttag aagctctttt aaatattctt aaaaattgta
     2401 gtgagcatat agaagatggt ggatatatag ttgcttatta tagtcaaaat cctctatatg
     2461 ttgagattat catagaagat aatggtaagg ggtttgctca ggaagaactt ccatatattt
     2521 ttaatagatt ttatcgtggt aaaaatgcta gtaaggacag tattggtata gggcttgctc
     2581 tatcaaagtc tattatagaa cgtcaaaatg gtactctaca tgcagaaaat tgccataatg
     2641 gtggtgcaag attcataatt aaattttaca cataaaatgc tggattatag gtaaaatacc
     2701 tataatccag ctatatctat attctatgtt atctattgtt ttagtaatat acttctattt
     2761 acatttaagg ttttattaaa caaacaactt aactatccct atacttgtaa gtagtcttaa
     2821 atcgaaacct ttaaataagt tcaaatagat tttgtttata ttgtttcatt ttttatagat
     2881 tctactatat tttcattctt caactgcttg tatcctgtca tatatgatgc tattacaata
     2941 aaaagtatca atatagtaaa acctattatt gtagatatag gtgcaaatgg taaatactct
     3001 ataaaatata tttcatttat acgtagaaaa gcatacacta ttcctatttg cacaggaata
     3061 ctaagtagta atggtgtaat tcctaagaat aaaccttcca aaataagcat cttttttatg
     3121 ccttctggag ataatccaac agaacgcaac atagcaaact cttgtcgtct acttctaata
     3181 tttcctgaca ctgtagcaag tacatttgat aatccaatca tagccaataa acctgtaata
     3241 aagcctacta ttaaattcat ggtctttatt ccactagcct tttgtttttc actttctaaa
     3301 acatcctcaa tactataatc tcctgaacca tagtctttat tataaatgga ctctaatttt
     3361 tctcttactc taggaatgtc ctcacgatta tttactttaa atactcctat cagcctattt
     3421 gaacgtaacc tacttacttc atcataattt ttagatattt cctgcaccat agacattgga
     3481 actatttgca ttaatgtata ctttgaacta gtatctacta ttttaggaag tgtgcttgta
     3541 atagctccca ctttcatgtt gtaagtataa tcccctttat cttcgtcata tactttttca
     3601 tttaatttaa tgttatctcc tattgaaaga tttaaataat ctatatatac tttatttcta
     3661 cgtgtactac gattaatatc ttcctcctta ttatatacaa ttgaggttgg attttttgta
     3721 tcataaaata actttgggtc tacagaaatt tgcctacaat attctgtaaa actcttatca
     3781 tcaagcccaa ttatatttga aattatacga aacttgccat cacgttctat aggagagtac
     3841 tttttagatg aagctatatt tttaaatcct ccatttttcg caagttcttt tgaagcatct
     3901 ttttcagtca accaagtgga agcagacatt ttttgataat atagtgaact ttttacacct
     3961 tctgtattat ctaatttttc aatgaaagca gactctacag gttcactatt ttccaaataa
     4021 actgctatat ctctttggtc cttataatta tcacttccat aaacagcttt tgctccttct
     4081 tgatttgcaa ttatatgcaa aaaacatgta agtaataaaa aagataatgt taaagatatt
     4141 gttgcagtac gataagattt tttacgagca cgtagtgcgt ttgatgcaag ttctccttct
     4201 attccaaata tctttcctaa tgaatattta tgcgactttt tgattttaac actatctcct
     4261 tgtcgtattg cttctatagg agatatcttt gatacttttc gtgctggtat aagtgcagaa
     4321 atccagactg ttactattgt aagtaaaact gctggcagaa aagcaggaat accataagta
     4381 aataccactt ctggaacatc agtatgataa ttaactgaat taacataaac tataagccta
     4441 ttacacaata accatcctaa aaataaacct attggtaatg gtataattgt cagtaaaaag
     4501 ccttcaaaca ctacagacct tttaatttgt tttggtgatg caccaacact agataatatc
     4561 cctaattgag taagtctagc atttgcagac agtgaaaacg cgttgtaaat caccattaca
     4621 aacacggcta ctgtaaaaat tgctattgta agatacataa taggagttga aaattttgat
     4681 atactactca tagatgcttt ctgttctggt gaaaagacta acatctttaa aagataatta
     4741 ctattgtatt taagattgta ttttccatat tcattagtct tccatcccaa tgatttagca
     4801 agttttggaa gttccttata cgtgtctctt atatttttaa atcgcaagta cacagttata
     4861 ctatcattag gttttatact tgttttatca agatatccaa gagctgtata tgcaggtatt
     4921 actgatgaag tagaaacatc tacctttcct actattgtca atgtagcctc tgaaattttt
     4981 ttaaaacttt cccctttcga ttttggggct atgggttcaa gaggctttcc atttaggatt
     5041 ctatctccta ttgggagagt aattttatca ccaattttta cacttggatt attctcaaaa
     5101 tattgttttg ataatgcaat ttcattagca ttatttggtc gacgaccctc tattatagta
     5161 tccttttctg gcatagaatt ccaatactct gatgtagcat ttctataaat tatataatct
     5221 cttttctcat cattaatttt gcctacatac cattgtcctt ttaccattac atcatcaact
     5281 gaagaaaaat tttcaattaa tggtaagtca cttccataag tatagtcaaa caattcacca
     5341 tgccagttac cttctttctc aattgaaagt tttataaaat ctgtccacat ggtatacaac
     5401 agtccacaca aagaagacat catggttgta gtaagtaata tagctaacat tatagctata
     5461 ctactacgtt tattgcgtcg tacaaagtct aaggtgtatt cttgaagaat attcatttct
     5521 tcaccacctt ttcagagaca atcactccat cttccatagt tacaatacga tttgcctcta
     5581 gtgctatttt ctcatcatga gtaataagaa gtattgtctg attaaatcgt ttatttgata
     5641 agtttaacaa gtctacaatt tcttctgaat tttttctatc aagattacca gttggctcat
     5701 ctgctaataa aatagctggt ctatagatta aacttctagc tatagatacc ctttgttgtt
     5761 gtccacctga aagttgactt ggaagatggt ttaatctatc agataatccc aatatagata
     5821 caatctcaga gaatctatcc tcatccacct ttcttttatc aagtaacatg ggtaataata
     5881 tattttttct aacatcaagg gtaggtataa gattataaaa ttgataaata agacctactt
     5941 ttctacgtct aaaaactgca agagcttctt cctttaaact tgatatttct gtatcttcaa
     6001 cataaatctt tccagatgta ggtctatcta ctcccccaag cagatgtaac aatgtagatt
     6061 tgccagaacc agatgctcct acaatggata caaaatcacc tttttgaact gataaattta
     6121 cattattaag ggctgtcact ctggtttgat ttgaaccata tatttttgtg agattttcac
     6181 attttaatat ttccatatac aatcatctcc tttatggtta aattttagca acctaaaatg
     6241 acattatagt gacatgaata caaatataaa ttactattaa aaatattgtt tagtttttgc
     6301 acattattta aaatta
//
LOCUS       DAMJZE010000201         6213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJZE010000201 DAMJZE010000000
VERSION     DAMJZE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.205"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            893..1654
                     /locus_tag="PC541_003392"
     CDS             893..1654
                     /locus_tag="PC541_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2840813.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1719..2501
                     /locus_tag="PC541_003393"
     CDS             1719..2501
                     /locus_tag="PC541_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2840814.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2768..3187
                     /locus_tag="PC541_003394"
     CDS             2768..3187
                     /locus_tag="PC541_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2840815.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(3290..3556)
                     /locus_tag="PC541_003395"
     CDS             complement(3290..3556)
                     /locus_tag="PC541_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2840816.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3783..4484
                     /locus_tag="PC541_003396"
     CDS             3783..4484
                     /locus_tag="PC541_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840817.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4566..5174
                     /locus_tag="PC541_003397"
     CDS             4566..5174
                     /locus_tag="PC541_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2840818.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5498..5962
                     /locus_tag="PC541_003398"
     CDS             5498..5962
                     /locus_tag="PC541_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2840819.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         2550 a          568 c         1063 g         2032 t
ORIGIN      
        1 gtgaaattta tctatagtat atttttataa acaagtaatg ttattaaaat taagtatata
       61 gttaaaagtg tatagcaaaa aggctgttat attaatgtta gaggttcagg gaaaatatat
      121 aaagcaaaga tatttacaga taatgtagat tgcacagcta taagttagat aatagaatta
      181 tgtaatcaag aatttacaac aaagtataag ataaggataa tgctaaattg ccatacaggt
      241 gctagatgtg taaatggatt taattaatac tattgataaa gtgcaacaag cacataaacc
      301 aattgatgag attattgaaa ctataagaga tatagttgac ataattaaaa tattggaacc
      361 tgtttataat tttaaatcaa atcaataatg attaagatta aaataatatt aaggcatcaa
      421 tgttataaga gtacaatata aggagtacta tatagtactc cttacttata catattaggc
      481 aatagggaat ttttttatag ttaaattttc aaatattttt tagggaactt tataattgag
      541 aattaagggt atagtatgtg atgaacttac taaatttata attaggttat ataatggaat
      601 aatcatcaat agttttactt tagggaaaat atatgcttaa ggaagtatag cttactaata
      661 ttattaaaat gtatatttaa aatgataaaa aaagaattag acaagtattt agtaaattga
      721 ctatgcaaaa ttctatcaag caggaacact atgactttag tcatgggagg ttcagtaggt
      781 aacttgttga attaagttta tattatttaa ataaactact taatttagat aatataaaaa
      841 gtcagaattt taagaataga gctatataat cttagctatt aggagaagta ctatgatagg
      901 aatatatttt agtggaacag gaaattcaag atactgtgta gaaaaatttt tacaagaata
      961 tgatataaca gcaaattctt attctattga agataatgaa cttttacagc acatcaataa
     1021 tcatgagaat attattttta gttatcctgt gcaatatagt aatgtgccaa aaatattgaa
     1081 agattttata ataaatcatt caactttgtg gaatggtaaa cgtgttttta ttattgcaac
     1141 tatggctcta tttagtggag atggctctgg tgtgttagct agaatattaa aaaaatacgg
     1201 tgctataact gttggtggat tacatgtgaa gatgccagat agtataggag atgaaaaggt
     1261 attaaaacat tctttagagt ataatagaaa gcttgtaata aaagcagaaa agaaagtaac
     1321 taaagctgct aaaaaactga aacatgggtc accaccaaaa gaagggttgg gttttttgag
     1381 tcatattgta gggttatttg gacaacgatt gtattttatc aataaaacaa atatgtatac
     1441 agataaacta aaaattaata ctgaggaatg tattgtatgt ggtaaatgcg ttaatttatg
     1501 tccaatgaag aatctagtaa ttaaaaattg tatgattgtt gcaaatgggc aatgtacaat
     1561 gtgttatcgt tgtgtaaata attgtgccaa aaaagcaatt acattgttag gtaaaaaagt
     1621 tgttgaacag agtaatatta aaaaatatat gtaagaaatt taaagtttta ttgaatgtaa
     1681 attgatgtat attttatgtt agaaagagag gttaaataat ggaatactac atagttgatt
     1741 catttgcaac taaactattt aaaggaaatc ctgctggagt ttgtgttctt gacagaagaa
     1801 tacctttaga gttaatgcag aaaatagcag aagaaaacaa tttaccagaa acagcatttg
     1861 tagttaaatg taaaggaaat tatgaattac gatggtttac tcctaaagca gaaattgatt
     1921 tatgtggtca tgcaacttta gcagcagctt atgtaatatc aaattttatt gatgttaatg
     1981 taaaaaggat agatttcttt actcaaagtg gaaagcttga agttacacga aatggaaatc
     2041 tatatgaaat gatatttcca gaaataatgc ctattgaaat agagttatca ccacaacaag
     2101 ctaatttgat aggctgtgta ccaagtgctg tttattcttc aagagattta atcttattgc
     2161 taaacagtga acaagaagtt ataaattata aacctaatta cgcacaattg cgtaaattga
     2221 cagactggtt aggtataata atcacagcac aaggaagtaa tacagatttt gtatcaagat
     2281 atttttgtcc agaattagat tcagaagacc ctgttactgg ttcatcacat tgtaatctta
     2341 ttccatactg gtctgaaaag ttgggaaaac acaaaatggt agctgctcaa ctttctaatc
     2401 gtggtggtat aattcagtgt gaagtgttga aggataatac tgtaaaaatt tctggtgaag
     2461 cagtcttgtt tatgcaggga acaataaaga ttgatattta aataagtata ataactaatt
     2521 tattatgaaa aataattaat tttcttagtg ttaacaataa taaagtagag ttaaaaagct
     2581 tgatttcact taaatcaata tgatagatgt tgatactaag attgacaagc atgattaaaa
     2641 aagataatat ttaatctata aagataaaaa cacagtctgg ttggtagtcc agacgcagca
     2701 atagctgtca gtaacctgcc tccttggttg tccgttcttt agaaagtaat ttaaggagga
     2761 actagcaatg gacaaaatta gtggaaaatt aacagtttta tttgaagaac cattttgggt
     2821 aggtatattt gaaagacaag atggtaaaaa atatgaagca tgtagggtag tttttggagc
     2881 agaacctaaa gaagtagaag tgtatgaatt tatacttgaa agatttttct cattagattt
     2941 tggtagcatt aaattagaaa aaaatgtgac caaagacaac attggataca aaagaatgca
     3001 gagaaaagta aaaaaggaac aagaaaaaga aactataggg actaaggctc aaaatgctct
     3061 taagcttcaa tatgaagaaa gaaaacagga tagaaaaaac ctagctaaaa atagaaaaga
     3121 agaagaaaaa gaaagactat ttaatttgaa acaagaaaag agaaaagcta aacataaggg
     3181 tcattaattt taattaaata tctttttaat aattgctaga aaaaggtgta taaaattttt
     3241 aattttatac accttaattt attgaaaaga ataaggtttt atatttcaat tactttaaaa
     3301 tataaaaata tactaataaa aattgtaaaa atagtataaa tggtatacca aatgtaaatt
     3361 taatgtgttt agttttatgt ctaaaagaat acattccaat tatagaacct atactacctc
     3421 caattactgc tatagacata agagtagctt cttttattct ccattcgttt ctaattgctc
     3481 ttttcttatc tatgtacatt gaaaagaaag caatagaatt aatcactata aaatatatta
     3541 gaataaaatt tttcacagtc ttgcctccat aaaaatatat tatacattat aaaacaagat
     3601 atttgttttg gaaagttaaa tttgtaatta gtacatataa aatatttgat aagaacaaat
     3661 ataatattaa ttggaaaatt taaaaaaatt aaacaattag caaatagggg gtatatttat
     3721 aagacacaaa gtgataaaat ctatttagaa aataatgtaa aataacagga ggataaagca
     3781 aaatgaaatt tatagtagaa aaagaagttt ttgataaatt agaaaatgta tgctttggtg
     3841 tagtagttgc aaaaggaata gataatacta aagaaataga aagaataagc aaccttttag
     3901 atataagtat agatagagta gaagattact tcaaagataa aaaagtaaag gaatcagaag
     3961 aaattatacc atatagagag gcatttagaa gtctaggcat gaacccaaat aaatttatga
     4021 gttcgataga agcaatgaca acaagggttg ctaagaataa gaaacttcct catataaatc
     4081 ctattgtaga tttaggaaac tctatttctt taaaatattt acttccaatg ggtgctcatg
     4141 atatggattt tagaaatgat gatgtatatg ttagattttc taaaaaaggg gataaatttg
     4201 taccatttgg agaaactgat gttgaattaa tggaagaagg agaattaatt tattcagttg
     4261 gagacatggt aaaaactaga agatggatat ggagacaagg agaggaagga aaaattacta
     4321 atagctcaaa aaatatattt tttcctatag atggttttac tgatgctaat ttagataagg
     4381 taatgagtgc aagagaagaa ttagcaaaat tgttaaagga aatatttaat tgtgaaatta
     4441 aagtaggttt tgtagataaa gataatcctg aaatggaaat ataataagtt gttgaatact
     4501 tgttaagata ttatatttac ttaatgatgt taaaaaggta ttaaaaaaga aaggtcgtag
     4561 taaatttgaa aaaaactata ataaagaatg taaatccaat gattgaaaat aagtttatag
     4621 gactttttga aatagagtat aaaaacaaat tagatgaaga taaggtttgg atggtagcat
     4681 caagaaaaag tagtgagcaa ttaaaaaata tttatttgaa gaatgaagaa gatagtgtag
     4741 atgcagttgt aatagttggg cttcataaaa gtagtaaaaa attaatttta ataagacaat
     4801 ttagagtacc tattaatgga tatatatacg agttaccagc aggtcttgtt gatgaaggag
     4861 agtctataga tatttccgtt gaaagagaac ttagagaaga aacaggactt actcttttag
     4921 aaattaaaaa aaataaaagt attgataaag tttatttatc tcctggaatg agtgatgaat
     4981 ctgttgcctt tgtttattgt atatgtgatg gtgatataac agatgaattt ttagaaccag
     5041 atgaagagat tgaagcgctt ttaatttcgc aagaagatgc aaaagagatt ttacaaagta
     5101 atcataagat agacacaaaa gcatttttga ttttacaaat gtttgtgagt ttaggagcta
     5161 aattatttga atagatatat agaaaagagg ttatatagaa gagtaaaata ataaagttta
     5221 ttttacttgt ataaattata atctcttttt ttatacaata aagtataatt ttgtgtattt
     5281 ttaaataagt gacttgactc ttaaattttc ataatatgtt ataaaccaat ttaagatact
     5341 acaataatta aaattccaaa attatttaac atatttgcaa ttaaaatgag aaaaggtaaa
     5401 ggttgaatga ttgaaaaatt tgatgtaaaa tagtagctta gtttaatatt atagtttgta
     5461 gggttaaaaa ttagaaattg taagggagat aaattctatg aaaaaattag ttattatttt
     5521 aattagtatc attagtatgg ttagcttgac agcttgtaaa aaaagcaatg aaagtaaata
     5581 taataattta aacaacaaag aatccggaca aacaaccatt gaaatggact tagataaaaa
     5641 ttatgatact tcagacccat ttgttaatgc acgtttgttt tgtgtgtcta atgatataga
     5701 tgttttagac acagagattt cttttaaaat ggatggagat agtggtattg tggaaataaa
     5761 ggacaataaa acatatgaaa ctttatggag taatacatgg catggaagcg ttgataatga
     5821 tacattcaca atttctcttt ctaatataca aaaagagaaa gagtatgcta tatgctttac
     5881 tggaattaaa ataaatcatg cagttgtaaa ggttactttt gaaagcaatt tagttaagga
     5941 gaaagaaaaa ccctcaaaat aaattccaga ttgtctataa cttgatattc agtacataaa
     6001 agcagttgat atttatgatt gactgctttt tcttatcaaa aaggagagtt tgtgcatttt
     6061 tacttgaaat agtttatgca catcaaaagt tggtgagcat gatattggaa atggtaagca
     6121 tgaaatcttg ataagacaag gaaataaatt taagttttca attaagtata tttccataat
     6181 ttagttaaca ataagctaat tattatttat att
//
LOCUS       DAMJZE010000202         6212 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJZE010000202 DAMJZE010000000
VERSION     DAMJZE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.206"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            85..630
                     /locus_tag="PC541_003399"
     CDS             85..630
                     /locus_tag="PC541_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2840820.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            643..1656
                     /locus_tag="PC541_003400"
     CDS             643..1656
                     /locus_tag="PC541_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2840821.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1878..3851
                     /locus_tag="PC541_003401"
     CDS             1878..3851
                     /locus_tag="PC541_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2840822.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3920..4279
                     /locus_tag="PC541_003402"
     CDS             3920..4279
                     /locus_tag="PC541_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840823.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4314..4670
                     /locus_tag="PC541_003403"
     CDS             4314..4670
                     /locus_tag="PC541_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2840824.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4707..5999
                     /locus_tag="PC541_003404"
     CDS             4707..5999
                     /locus_tag="PC541_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2840825.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2453 a          580 c         1182 g         1997 t
ORIGIN      
        1 tacagaaaaa agatgtaatt tgtaaaattt atggtaagca gtaaaattaa aaatttaaag
       61 atattatagg gaggttataa ttttatggaa aacgttatta caggtctaag taaaggtgca
      121 gagtggttta tcggtttgtt tcagaaaggg ggagaacaat ttataggtct tgtttcaggt
      181 actcttccaa cgctaattgt attaatggta gctattaact ctttgattaa gataattggt
      241 gaggaaagag ttaataattg ggcttctaag ctaggaaaga actcttttac aagatatatt
      301 ctacttccat tagtatcagt atttttcttg ggaaatccta tgtgctatac atttggtaga
      361 tttttaaaag aagagcacaa agcaggattt tatgatgcag cagtttcatt tgtacatcca
      421 attacaggtc tttttccaca tgcaaatgca ggagaacttt ttgtatggtt gggaatatct
      481 gcaggtttga ctactttagg taaagaaact actacattgg cattgtggta ttttatagtt
      541 ggtctaattg taatttttat aagaggtata gtaacagaaa aaatgtatgc ttttctaaca
      601 agaaaaaata gagcaacaaa taaggcttag gaggtagata aaatggagga gaataaaata
      661 ttaaaaatag aaaagggaac ttcaggctgg ggaggtcctt tgtatataaa aaaagaaggg
      721 aatagaaata agatactatc aatgactgct ggcggtatcc atgaagtaac attaaaaata
      781 aaagaactac tgggttgtga aattgtagat ggatttaaaa ctggagtttc tgatgaagaa
      841 gtagcagtag ttattattga ttgtggtgga actgctagat gtggagttta tccaaagaaa
      901 aaaataccta caataaatgt aaatccagtt ggaaaaacag gtcctttagc aaaatttata
      961 acagaagaat attatgtatc agatgttaat cctaactgta taagtgttgt agatggggaa
     1021 gatatgccac aaaaatctca agaaaataaa agcgaaaata agagttctat tagaaagcct
     1081 gataattatg atgaggttaa gtcaaaagca caaggtgaat atgctaaaaa gaacataata
     1141 ctttctattg gtcaaggggc agggcaagta gtatcaaaat tttatgatgc aggtcgagat
     1201 actatccaaa tggtaatgaa taacgtaata ccatttatgg catttgtaag tatgcttatg
     1261 ggaattattt tagcatctgg actgggagac tggattgcaa gagttatatc tccacttgca
     1321 ggtaatattg gtggattatt gattatatca gtaatttgca cacttccatt cttatcacca
     1381 atactaggac ctggtgcagt tatagctcaa gtagtaggga ctcttgtagg tacacaaatt
     1441 ggtcttggtg caataccagc atatttagca cttccagctt tatttgcaat aaatgggcaa
     1501 gctggatgtg attttgtacc agtaggatta agtttgggag aagcagaacc agaaactgta
     1561 gagtatggtg ttccagcttt attttactct cgtttaataa ctggtccaat agctgttatt
     1621 attgcatatg gagttgcagt atttgcattg agataatatt taatgtcaat ataaggcttg
     1681 gaattaatta ataaaattgt aaatataaat ttaagctagt caagattttt tgttaatgaa
     1741 agactagctt ttatactatt aattagctgt aattatcgag ttagattaat actaaatttt
     1801 cctttattaa agtgaaattt tatgtattaa tttggtttca aatttgtact agaatgaaat
     1861 caaggaggag atacaaaatg agtgtaactg agaaaattcc ttctattgac cgcaagatat
     1921 ttaagatatt gattatgtgt agtaaaaaag agtttgtgag tattaactca atagcaaatg
     1981 aattgaatgt cacaactaga agtgtaagga cgtatataaa acaattgaat aaagatttag
     2041 gtaatgatat agcagtaatc aaatatataa agggtcaagg ctataaacta gaaataaaag
     2101 atgagcagat actcaataag attattgata tgaatagaaa aaatgtattt tcattaaatt
     2161 caaaagaaga tagagtagag tttatactaa attacttgat agaactagat ggatttatta
     2221 cacttgatag tttagctgat gagatgtgtg taggaagaac tactttagta aatgatttcc
     2281 agtatgtaga aaaagttttg gcttcatata atttaaactt gattaaaaaa cagaatacag
     2341 gtatgaaatt aaatggaaat gaacttgata ttagactgtt tatattaaat cagttgtata
     2401 aaaactcaag gaaagatttc agtaattcaa agtattttaa aggaatcaaa aaagaagaaa
     2461 tcataaattt agaagaaaaa ctattgactt tatttaaaaa aaataatttt tatgttacag
     2521 atgaaatgtt aagagaagtt attaactaca tagtagtttt agtctataga gtaaaagaag
     2581 aaaagaaagt aaaagattat gatgtaaagt ttgatttact taaatcatat gatgaatact
     2641 ttattgctag ggaaataaaa catataatat caggaatgtt tgaatgtact ttaaatgatg
     2701 aagaaattat ttatttaact ataccgttag ttagtggaaa tgcaccagca tcagaatgtg
     2761 ctttaaatag tagtagaatt agtaaaaaca ttgatgagtt gatggaaaat atatttaatc
     2821 aaatatatgt cgatatgggt atttttataa atgaggatga attaagagta ggtctaggat
     2881 atcatcttag ttttacttta aacaggctat tatttaatat aaagttaaaa aatgttttgt
     2941 tagaagagat taagcaaaat tatatacttc catttaaatt agcacaaata gcaggaaaag
     3001 taatagagaa aaagtataac ttagaagttt cagaagatga gattggatat atagcaatcc
     3061 atttcagtgg atatttagaa agaaatagca gtagattcta ttcaataaaa aaaatagcaa
     3121 ttatatgtag tacaggtcta ggaacagcaa aactattgaa aataagagta gaaaaactga
     3181 taggaaataa tcctaaaatt gatactcttt caagttttaa tttgagaaat attaacttag
     3241 atgaatacga tatagttttt acaactattg atttagacac atcaaatata aatacaatag
     3301 tattaaaaat aaatacaata tttgatgaaa acaaactaag agaacaatta aaaacagtat
     3361 tgtgcctaag agaagggaat attgacacta ctaattctac aaatttacta ataaataatt
     3421 tgttagatga agataaattt atgatattaa atgaaaagac tatacttgcc tcattagaaa
     3481 aaatgatgga ccatcttatg gatttgggat gtattgatga gaagtttaga aaaaatatag
     3541 tcaatagaga agaaaaatct cccacagtat ttgataaagg tttattattt cctcattcta
     3601 taaatgaaaa atcagataag tttttaatgg cagttggaat tttagaagaa ccaatagaat
     3661 atgcaaatag aagtataaaa ataattctta tgacaatgtt tccatgtgag aataagatag
     3721 actcagactt attagtaaaa atttatgaag aagttttaaa gataggccaa gatgtaaaat
     3781 taacgaataa gataagtaaa tgtagaactt ttttagaatt taaaaaggtg ttacttaaaa
     3841 accttatata actattgtac agcatttata gttgttatac agatttaaaa ataatgtgat
     3901 ttttggaagg tgtataaata tgaaattatt agtaatttta tttgttgctt atattttaaa
     3961 tactatattt ataatgaagc agaataaatt gtattttaaa actttaaata gagctaaaaa
     4021 aaagggtgat atagtgtcta caggcaaaaa gaaaagctat ttttctaaag gaagtatagc
     4081 cgttatatct tcagattcag atgggtttat aaagtttgga gaaattttaa aagggcgtac
     4141 agtaatggct aaatttaaag agattgaaga aatagaagga ttagatatat atagtgcaga
     4201 gcaaaaattt aaagatgaag agagtattgt acaagctata aaatttataa aagaaaaaat
     4261 aaatgtttct tttaattaac tcaatataaa tataaattat caggaggaat gtcatgaaat
     4321 atgaagttaa gataagtggg ataggcagtt tagttaaaga gttaatggat gaaagcaatt
     4381 gtcttattat atatgatgaa accattaatg atgacgactt aaaggatata tctgtaattc
     4441 attcaatagc aacattaaaa agtgatgttg aaataggaga tacacttact atagggaata
     4501 gagattattg tatagtttca gttggtgata ttgctcagaa gactcttaga gaaattgggc
     4561 attgtacaat caaatttgat ggaaaatgtg aagtaaattt accaggagaa atacatgtgg
     4621 aaataggaaa tccagatata actattggag atttaataac tatttcttaa tttgaaaata
     4681 aaattattat aacaggagga attggtatgt tagatttaaa cagaaaactt gcaaaattgg
     4741 aagaggaagg taaaaaaata aaagtagctc ttataggagc tgggcatatg ggaaatggaa
     4801 tggtaagcca aatggcaaat atgaaaggta ttgaagcttc tattgttgta gatatagatt
     4861 tggagttggc tcataaagct tttactgatg caggaataga tgaagaaatt atagataatg
     4921 taacaaatga aaaggatgca gaaataaaat tacaagaagg aaaagtattg acatgtaatg
     4981 actttcttgt ggcttgtaaa actaagtcaa tagatgtagt tatagatgca actgggggta
     5041 ttgcaattgg tgcagagata gcattaaact ctattttaaa taaaaaacat atagttatgt
     5101 taaatgctga aactgattgt gttgttggac caatacttaa aaaattagca gatgatgctg
     5161 gagtaatatt tacaggttct gcaggagatg agccaggtgc agtaatggaa ttatttgatt
     5221 ttgcagatgc aatggggttt gaagttagag tgataggtaa aggtaaaaat aataaattag
     5281 atttagattg taatcctgat acagttagag aagaagcaga aagaaaagga gcttcacctc
     5341 atatgatagc ttcttttaaa gaaggaacta aaacaatggt agaaatggct ttaatgtgta
     5401 atgcaactgg atttgtgcct gatgtaagag gtggtcatgg tattgaagct acagttaatg
     5461 aagtacctaa gaaatatgca ttaaaaagcg aaggtggagt attagataat tatggtgtag
     5521 ttgattttgt aaatggtata gcacctggag ttttcgtagt agttgcccat aaattaaaag
     5581 cagttaatga tgaactaaaa tatttaagta tgggtgatgg acctaattac atattatata
     5641 gaccatatca tttatgtagt ttagagactc ctttatcagc agctatggca gttttagacc
     5701 ataaggctac aatagtacct aaagctggac tagtagcaga agttatgact atagctaaga
     5761 aagatttgaa aaaaggtgag tttatggatg gctatggagc atatacatgc tatggtacta
     5821 tagaaaaata cgatgtagca aaggctatga atgctgtacc tattggtttg ataagtaaaa
     5881 agactaaagt tgtaaaagat attaaaaaag gtgaagttat aacttacgat atggtagaga
     5941 tagaaaaaga tactacttta tatcatttaa gacaattgca agaaaaaata tttgggtaag
     6001 atttgatggt aatataaata gatattagta aaaagggttg atggttattt ttaaatcaac
     6061 ccttttaaat ttttatatat tcttgttagg atactttctt taaaagtata ttataggtta
     6121 tttgtagtcg taaaaattat tttgaattag ttctgcaatt ttatattcac attgaaataa
     6181 tattattaaa aattttaata taaaatgaat ta
//
LOCUS       DAMJZE010000203         6196 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJZE010000203 DAMJZE010000000
VERSION     DAMJZE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.207"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            187..1209
                     /locus_tag="PC541_003405"
     CDS             187..1209
                     /locus_tag="PC541_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2840826.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1269..2276
                     /gene="gap"
                     /locus_tag="PC541_003406"
     CDS             1269..2276
                     /gene="gap"
                     /locus_tag="PC541_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2840827.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2498..3700
                     /locus_tag="PC541_003407"
     CDS             2498..3700
                     /locus_tag="PC541_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2840828.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3726..4469
                     /locus_tag="PC541_003408"
     CDS             3726..4469
                     /locus_tag="PC541_003408"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2840829.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4478..6010
                     /locus_tag="PC541_003409"
     CDS             4478..6010
                     /locus_tag="PC541_003409"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2840830.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2468 a          728 c         1248 g         1752 t
ORIGIN      
        1 taaaaagaaa aaatactgtg atttgaaaat ggtggagaaa tattttataa aataaaaaaa
       61 taaaaaagtt tttgaaaaac tattgaaatc tatacgcaaa tcctataaaa taaaaatatc
      121 ttgtgggact taaataaaca ctagggacaa aaaaagtccc gaaaggaaag caataaggag
      181 tatgttatga agaacttatt agaaattcaa aagaaattga ttccacaggc aattgaactt
      241 atggaaagaa gatactctat tttaagacaa atatctttaa gtgaaccaat aggtaggaga
      301 acattatcaa atgtattaga gattagtgaa agaataatca ggtcagaaac tgagttttta
      361 aaagaacaag gacttataga cgtagctgta tctgggatga ctatcactaa agaaggttct
      421 gatttgttag ataaacttaa ggacatcatg agtgacatca tgggattgtc taagttacaa
      481 gatagagtca gagaaaagct tggaataaaa agggtaatat tagttcctgg aagctgtgat
      541 gaaaatggaa gtttactaaa agatgtaact agatatggat gcgaatattt cttaggcatt
      601 ttaaaagatg gagatatcgt ttcaataaca ggaggaagca ctatgttgga gttttcaaac
      661 gttattaaaa ctgataagag atatccaaac tctacaatag ttccagctag aggaagtatg
      721 ggaactgatg tagagatgca atctaacaat atagttgctt caataagtaa gaaattacat
      781 tctaattgca aacttttaca tataccagat gagttggatg aaagtgcaat gaaaactctt
      841 agccaagtgc cagagattaa gacaactttg gagtatatta aaagaacaga cattttagtt
      901 tttagtatag gtagagcaga tgtcatggca aagagaagaa aacttccaga agacaaggtt
      961 gatgagatat tatctaaaaa tgcagttgga gaagcatttg gatattattt caacaaagat
     1021 ggagaaatag tttacaaact gaacactgtt ggaattgatt tagaaacagt taaacaagta
     1081 aaagaaacta ttgcaatctt tgcagggact gaaaaagtag aagccttatt agctatatca
     1141 aatatgcata aaaacatggt tcttgtaaca gatgaagaaa gtgcttataa gatattgtca
     1201 ttattataaa agtcaagacc tagcacagag ctagaaaaca tacatttact ttataggagg
     1261 tagcgaaaat ggttaaagta gctataaatg gatttggaag aataggaaga ttagcattaa
     1321 gaaaaatgat ggagcaacaa gataagttcg aagttgttgc aataaacgac ttaacagacg
     1381 ctaaaatgtt agctcattta tttaaatatg atacagctca aggaagattt aacggagaaa
     1441 tagaagttaa agaaggtgct tttgtagtaa acggaaaaga aataaaagta acagcagaaa
     1501 gaaatcctgc tgacctacca tgggctgaat taggagtaga tatagtatta gaatgtacag
     1561 gattctttac atctaaagat aaagctgaag ctcatataca agcaggggct aagaaagttg
     1621 ttatatctgc accagctact ggagatttaa aaacaatagt attcaacaca aacagtgaca
     1681 tattagatgg ttctgaaaca gttatatcag gagcttcttg tactactaac tgtttagctc
     1741 caatggctaa agtattaaat gataaatacg gtatagaaaa aggcttaatg actactatac
     1801 atgcatacac taatgaccaa aatactttag atggtcctca tccaaaaggt gatttaagaa
     1861 gagctagagc tgctgctgga aacatagttc caaacactac aggagctgca aaagcaatag
     1921 gcttagtaat accttcatta aaaggaaaat tagatggggc agcacaaaga gtaccagttg
     1981 taactggttc aataactgaa ttagtttgta cattaggaaa aaatgttaca gttgaagaaa
     2041 taaatgcagc tatgaaagaa gcttcaaacg aatctttcgg atatactgaa gaaatgttag
     2101 tatcttctga cataatcgga ataagctacg gttcattatt tgacgctact caaacaaaag
     2161 ttatggaagt agatggaaaa caattagtta aagttgtttc ttggtatgat aatgaaatgt
     2221 cttatacttc tcaattaata agaactttag gatactttgc acaattagca aaatagtcag
     2281 ttttgagaaa atagaaaagt agattgacaa agtccgagtt tgtagtttaa tactacggac
     2341 tcaggacttt ttttaagaaa aaatatataa aatacaccta aataatgtta catactatat
     2401 ataaaaatat gataatatga tatatttaat agggtaaaag tatgtactat attatacttg
     2461 gcaataaaaa attactttat taaaaggaga gatatttatg tcaatgctta acaaaaaaac
     2521 tatagaagat attgatgtgt gcggaaaaaa agtattagta agatgtgact ttaatgtgcc
     2581 attacaagat ggtgtaatta ctgatgaaaa tagattgaac ggagctttac caactattca
     2641 atatttaatt tctaaagggg caaaagttat attatgctct cacttaggaa agccaaaagg
     2701 agaagctaaa ccagaattat ctttagcacc agtagcaaag agattatctg agatgttagg
     2761 aaaagaagtt gtatttgcag cagacgataa tgttgtagga gaaaatgcaa agaaagcaac
     2821 tgaaaaaatg gaaaacggag atgtagtatt attagaaaat actagatata gaaaagaaga
     2881 aactaaaaac gaagagaatt tctctaaaga actagcatca cttgctgaaa tatttgtaaa
     2941 tgatgcattt ggaactgctc atagagcaca ttgctcaaca gttggtgcag gcgagttttt
     3001 acaagagaga gtttgtggat acttaattca aaaagaatta aaattcttag gagaagctgt
     3061 tgcaaaccct gtaagaccat ttacagctat acttggagga gctaaagttt ctgataaatt
     3121 agctgttata aacgaattat tagaaaaagt tgataatcta ataataggtg gaggaatggc
     3181 ttatacattc ttaaaagctc agggttatga agtaggaact tctttacttg aaatagataa
     3241 agttgaatat gctaaagaaa tgatggaaaa agctaagaat aagggtgtta acttattatt
     3301 accagtagac gttgttatgg cagaccactt tgcaccagat gctactccaa tagtaacaga
     3361 agatgctaat gtaaaagaag attacatggg actagatatg ggacctaaga ctatagcaaa
     3421 ctttgtaaaa actataaaag aatcaaaaac tgtagtatgg aatgggccaa tgggagtatt
     3481 tgaatttgaa aactttgcaa atggtacatt atcagtagct agagctatgg cagagttgac
     3541 tgatgcaact actgtaattg gcggtggaga tagtgcagca gcagttaatc aattaggatt
     3601 tggtgacaag atgactcacg tatcaacagg tggaggagca tcactagaat ttttagaagg
     3661 taaagaatta ccaggtattg cagcattaga taataaataa actaatatat agaggagatt
     3721 gtaaaatgag aaaacctata attgcaggaa actggaaaat gcataaaaca ataaaagaag
     3781 ctttagaatt tgtaaatgaa gttaaagata aagtaaattc tgataaagta gaagctgtta
     3841 tatgtgcacc atttacttta ttaaaggatt taaaagaagc tactaagggt acaaatataa
     3901 agataggtgc tcaaaatatg cactttgaag aaaaaggagc atttacagga gaagtttcac
     3961 ctcttatgtt aaaagaaata gatatggact atgttgtaat aggacattca gaaagaagac
     4021 aatacttcaa tgaaactgat gaaactgtaa acaagaaagt tttaaaagca ttagaagtag
     4081 gaatagaccc aatattatgt gttggagaaa ctttagaaca aagagaagct ggaaaaacta
     4141 aagatgtttg taaagttcaa gttgaaaaag ctttagaaaa tgttcttaaa gatgatttag
     4201 caaaagtagt tgttgcatat gagccaatct gggctattgg aactggtaaa actgcaactg
     4261 ctgaagatgc taatgacgtt atatcttata taagagaagt tataaaagga ttatatggag
     4321 aattagctaa tgaagttaga atacaatacg gtggaagtgt taaaccttca aatgtagccg
     4381 aaataatggg tcaaagtgat atagatggag ctttagtagg tggagctagt ttagctagta
     4441 atgattacct agaccttgtt aatttctaag gagtgaaatg atgaaaaaac cagttgcttt
     4501 aattattatg gacgggtttg gatacaataa ggatgttaag ggaaatgcaa ttgctgagtc
     4561 caaaactcct aatttagata gaataaaaaa agagtatcca aacacattga taaatgctag
     4621 tggtcttgat gttggtctac ctgatggtca aatgggaaat tcagaagttg gacacactaa
     4681 cataggtgca ggtagaatag tatatcaaga tttaactaga ataacaaaat ctataaaaga
     4741 tggtgacttt tttactaata aagttttatg tgaagcaatg gataatgcaa aagaaaactc
     4801 tcttcatgta atgggacttt tgtctgatgg aggagtacat tcacatatag accatcttaa
     4861 ggctataatc aaaatggcaa aagataaagg cgtacaaaag gtgtatgttc acgcatttac
     4921 tgatggtaga gatactgacc ctcaaagtgc attagaatat gccaaagaag tacaagctag
     4981 tatggatgaa attggagtag gtgaatttgc tacagtttct ggtagatact acgctatgga
     5041 tagagataag agatgggaaa gagtagaact tgcatacaat gccatggtta gaggtattgg
     5101 tgaaaaagca aattctattg aggaagctat tcaaaactct tatgatgatg gaaaaaatga
     5161 tgagtttata atgccaacag ttattatgaa agatgataaa ccagttggaa gtataaagga
     5221 aaatgattca attatattct tcaactttag accagataga gctagacaaa taactagagc
     5281 tttagtttgt gaagagtttg atggatttaa aagagaagat attaaaaact tctttgtatg
     5341 cttaacagag tacgatataa ctatagagaa tgtgcatata gcatttggac ctcaatcttt
     5401 agccaacact ctaggtgagt atctagctaa aaatggaaaa actcaactta gagctgctga
     5461 aactgaaaag tatgcacatg taactttctt cttcaatggt ggagtagaag agccaaataa
     5521 aggtgaagaa agactattga taccttctcc aaaggttgca acttatgatt taaaaccaga
     5581 aatgtcagca tatgaattga cagataaagc tttagataaa ttaggagaag ataagtttga
     5641 ctttatagta ttaaactttg caaatcctga tatggtaggt cacacaggaa gtatagaagc
     5701 ggctataaag gctgttgaaa ctgtagatac ttgtgtagga agattgatag ataaaatagt
     5761 agagttaggt ggaagtgcaa taataactgc tgaccacgga aatgctgaat atatgttaga
     5821 cccagaaaca ggaaaaactg taacggctca ctctattaat ccagtaccat ttattgtagt
     5881 tggtcaagaa tatgaaagtg caaaattatt ggatggagga agattatctg atatagcacc
     5941 tactatttta gatatgatga agttagaaaa gccagaagaa atgactggtc attcacttat
     6001 ttcaaaatag tcgattctga taagtagata ataatatata aactacaata taaatttaaa
     6061 aataatgcag tttaggttta ttttgtaaag tttagacttt atgacataag gtttaaattt
     6121 tgtaagttct ggatattgta aggtgtagat gaagatatat tttataaaat ttacaagata
     6181 gacaatttat aaaata
//
LOCUS       DAMJZE010000204         6162 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJZE010000204 DAMJZE010000000
VERSION     DAMJZE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.208"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            287..1654
                     /locus_tag="PC541_003410"
     CDS             287..1654
                     /locus_tag="PC541_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2840831.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1679..2287
                     /locus_tag="PC541_003411"
     CDS             1679..2287
                     /locus_tag="PC541_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2840832.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2335..2706
                     /locus_tag="PC541_003412"
     CDS             2335..2706
                     /locus_tag="PC541_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2840833.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2885..3223
                     /locus_tag="PC541_003413"
     CDS             2885..3223
                     /locus_tag="PC541_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2840834.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3689..4192
                     /locus_tag="PC541_003414"
     CDS             3689..4192
                     /locus_tag="PC541_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2840835.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4239..5198
                     /gene="moaA"
                     /locus_tag="PC541_003415"
     CDS             4239..5198
                     /gene="moaA"
                     /locus_tag="PC541_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2840836.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5202..5672
                     /gene="moaC"
                     /locus_tag="PC541_003416"
     CDS             5202..5672
                     /gene="moaC"
                     /locus_tag="PC541_003416"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2840837.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5708..6139
                     /locus_tag="PC541_003417"
     CDS             5708..6139
                     /locus_tag="PC541_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2840838.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2603 a          501 c         1118 g         1940 t
ORIGIN      
        1 aatttttata tagtaaatga caaaagttta caataaatgt atattttctt actcattcct
       61 taaaattact catttagttt aataactctc atttcatatg taaagttggt aaaaaaatta
      121 aaattaaaag taaaaaacta aataaattaa tgttaatttg aaatatatat gaaggttttt
      181 ggagaatact tacttatgat aggaaaaaat gttaaaataa tagtgtaatc aaatataaag
      241 gactgaaata attaacagtt attaaatatt aggagtgtaa aaatttatgg gattaaaaga
      301 atttgatttt attgaagagt ctattgatat gttgaggaca atgtcaccaa cattagaaac
      361 tatatcagat gaaatagaag aatattttga aaatatcttg gatgaaaaga atcaagaata
      421 tataaatgta acctctagaa taaaatcaga atcaagttta agagaaaaga taattagaaa
      481 tagatattta aaaaaatatg gagaagcaag taatcttatt cataatgttt ctgacttaat
      541 aggcctaaga attgagtgta gatttattga ggatgaaaac aaaatatata gacttttgag
      601 aagatatttt aataaaacag atgacaaaat aaactattat aataaagaga ataagaatat
      661 taaattaaaa ctatctgaga ggcaacccaa taagcaaaaa aatggatttg aaatatataa
      721 aatagatgga gtttttgcgt atttagatag agaagtaaaa tttgaacttc aaattaaatc
      781 tcttgtaaat gtgttttgga gtgaaataga acataaaata atttataaaa acaatacata
      841 tttacttgct gataaattta taaaggatat gatggactct ataaagaata atcttactat
      901 gatagataat cagcttttaa gtatttataa aaactttcat tcagggaagt cttttaatat
      961 gaaagtaagt aaaaaagaaa ttgaaaaatt atttgcgaaa ctagtctatg acgctttttc
     1021 tgaaaagatg aataaaagta taggttttgt tgtagatttt aaaaaaccat gtgagactat
     1081 attgaactat tcttttaata aacaagatat aagtgatgaa gttttaggta attttatgct
     1141 tgatgagttt gcaagattaa atgaaattgt aaacaaggat atagatttta atgagcaaat
     1201 agaatttgaa agagaaccaa catttgatga taagttttgt aaagacctag gaaatcattt
     1261 tagaagtaga ttaaacacag aatttccatg gaacctattt tttagaatat tatttgaaat
     1321 agagccatat aacaatacca aagactttga aaactttgta gagtttataa aagaaaatgt
     1381 gctactagaa gaaaataggg agcaattatt atgccaattt gaagaggata gtaagcttat
     1441 aattgaagat atatatgatt gtatatttaa tagtataagt gagattgata gtgttgaaat
     1501 tttatataac tataatcttg aaaaaataaa ctttacatca agtgaaatca taaattgtat
     1561 atgtagagaa tatgagtgtt atggtgaata cttagaagaa aaagaaaagc ttatgaatac
     1621 atttaaagaa aagattattc aaatctttga gtaataggtc aatgctgaag tggcgctaat
     1681 gcgccatttt tcaaatatat atgaattaag taaaattatt ttggaggagg cgatttgtat
     1741 agagaaaaca ggtgtaattt tggcaggggg aaaaaattct agaatgggta gagataaagc
     1801 ctttttagag ttacatgata agctttttat tgaaattgca atagaagcat ttaaaaactt
     1861 tgatgagttg ataataatat ctaacaatga agaattatat tctaaatacg atattaaagt
     1921 ttataatgat atagtaaaag atgttggtcc aattggagga atatatactg ctcttgaata
     1981 tgcaaagtat gatatagtta caattgcatg tgatatgcca tatctaaata atcaaatagt
     2041 cgaaagaata gctaataaaa tggatgataa aagtgtgatt tcagtgacaa atggaaagtt
     2101 acaaccattg tgttctggat ataaaaagag tataataaat aaagtatctt tatgtataag
     2161 ggaaaatgat ttaaagctca gaagctttat agataaaata gataaaagtt atatatactt
     2221 tgatgaagaa gatttatttt taaatgtaaa cactgttgat gagtataaaa aattaactaa
     2281 agtttaatta gctatggttg taaaatagag taggatttat atggaggaga gtttatgaat
     2341 ttacttaaat gcgattcttg ggcagtaata ttaaataata gcgataaaga aagtaaagca
     2401 tataaaatat tagatgaact taaaaggaat atgtacaaag ttgttgctat agatgaagag
     2461 aaaaaaccta ttgaaggaat agatgtgtat gaatgtttaa aagatgttcc acacaatatt
     2521 gatgttgtag ctattattga taaacaatct aagatggatg tgattttaga agaagtagag
     2581 cttttggata tacaaaatat gtggtttgaa aaaggaagct ttagtgaaat gatgattaaa
     2641 aagactaaag atttaaaatt aaatatagaa tataatttaa gtttatatga tgagttgact
     2701 agataaaatt atatttctta ataataaaaa tataattagt taataaaaca tattatttta
     2761 tagtatagat tggttaataa aagtacagat tggttaatga aacattaaat tacaatattt
     2821 gttaaaaaaa ttgtgagtta catgatataa tgtattaaat aggatactaa ggagttgata
     2881 tattatgtta atagtaacta cagaaaaagt agaaggtaaa aagatatcaa aggttttagg
     2941 attagtgaga ggaagtacaa taagagcaaa acatgttgga aaagatatag gagcaagttt
     3001 taagaatctt gttggaggag aacttactgg atataatgaa atgctcactg aagcaagaca
     3061 aattgccata ggtagaatgg ttgaagatgc agaagctaaa ggtgcaaacg cagtaatagc
     3121 atttagactg tcttcagctt cagttatgca aggagcagca gaaatgcttg cttatggaac
     3181 agcagttgtt ttagaagatg ataatagtat tcttgaaaaa taaaatataa atagttaaaa
     3241 agaggcatct tttaaaaatt ttatggatgc ctttttttat ttgaaaaaac taatttgaga
     3301 agaaatttta actattatga caataaaatt agggtgatat tatataaata agtaaaatta
     3361 tattatataa tgtatggtat agactttaaa aattttattt gtagagaaaa atatggacaa
     3421 tgttataata attatagaaa ttaaaagtac ttacattcat tatgtatgtg acatgtataa
     3481 ttagttttat atttaataca gttttgaagt aatatcttca ttttgagaac gttggaataa
     3541 taaaaaatgt attattatag gtgatataaa taaaaaataa atataatatg ttatattgct
     3601 gatagaaaag agaataaaag aaaataggtt ttattaaaag agatttttat ttataggact
     3661 taatcaatgt atagaggggt gagaacatat gtttaatgtt gcaattataa cacttagtga
     3721 taaaggatat gaaggaaaaa gagaagatat aacaggaaaa aagttaactg aatttgttga
     3781 aaatacagga gtttataaag ttactgaata tgtacttata aaagatgata aggaaatgct
     3841 aaaggaaaat attataagac tttgtaatag taataaaata gatttaattc ttacaaatgg
     3901 aggtactggt ttttcaaaaa gagatataac cccagaagca actaaggaag tgttggaaaa
     3961 agaaatacca ggtctgagcg aatatatgag aatgaaatcc acagaaatta ctaaaaaggc
     4021 catactaagt aggggtgtga gtggaataag aaataattct ataataataa atttacctgg
     4081 aagtcctaaa ggtgcagttg aaaatctaag ttttataatt gatgttttag accatggcat
     4141 agaagtttta agaggagaag ctacagaatg tgccactaaa aaaagtgaat agtatataat
     4201 ctagatagat aaaaaataga caaagaggta gataaagtat gattgataag tatggaagaa
     4261 aaatagacta ccttcgtatt tcacttacag ataagtgtaa tttaaggtgt gtctattgta
     4321 tgccaccaga tgtaaaattt gataaaaatt atataaatga aaatctaagc tttgaagact
     4381 acaagtttat tataaaggct atggctgaac aaggtataac aaaaattaga tttactggag
     4441 gagaacctct tttatatcct aaattaaatg agcttataaa atttacaaaa gaagaatgtg
     4501 gaataaataa cataggaatg accactaatg ctattggtct ttctgataga atttttgagc
     4561 ttgaaaaatg tggcttaaat aaagttaata taagtttaga ttctttgaag gaatataagt
     4621 ataaatccat aacaagagga ggaaacttaa aagatgtctt aaaatcgatt gaatcttgtt
     4681 taaatttagg tataaaagta aagttaaatt gcgttgctat aagtgggttt aatgatgatg
     4741 agcttaaaga ttttatgttt ttaacagtta aatcaccaat tgatgtaaga tttatagagc
     4801 ttatgccatt aggtgaggct aatagagttt ataagaaggg ttattttaat gtaaaagaaa
     4861 tgattgaaaa aatatatggt ttatataaag tcaaaaatga tgaaaaaagt acagcagaat
     4921 attatatgtt taaaggagct aaaggaagaa taggaattat aactccatta agctgttcat
     4981 tttgtaacac ttgtaatcgt attagactta cttctagtgg tgcaataaaa ttatgtctac
     5041 attcaaaaga agaaatagat ataaaaccat ttttacataa accattgtta tttagagaat
     5101 ctatgaaaga tataataaaa caaaaaccag aaaaacatca tttaatagaa aataaatgta
     5161 gtgatacaaa taggcgtatg tatgaaattg gaggataagt aatgttaaca catataaatg
     5221 aaaaagggta tgccaagatg gtggacataa gtgaaaaaga tgatactaaa agaacggccg
     5281 tagctacagc tacaattact cttagtaagg aagcattaga aaaagtaaaa aatggtcaga
     5341 taaaaaaagg agacgtattg tctgtagctc aagttgcagg tataatggga gctaaaaata
     5401 catcttcaaa cataccaatg tgccatcaga ttaaattagt tggatgtgat ttagaatttg
     5461 atgtagatga ttctatgtct gctattaaga tatacgcaac ctgtaaatct cttgggaaaa
     5521 ctggagtaga aatggaagct ttaaatgcat gttctttagc agcacttaca atttatgata
     5581 tgtgtaaagc agttgataaa aaaatggtta taagtaatat ccatcttgtg gagaagactg
     5641 gtggaaaatc aggagatttt aagttcgagt aaattaataa tatattgggt taaaatttgg
     5701 aggaaatatg gcgaaggtag tgtcaataaa tataagtgag aaaaagggtg taattaaagt
     5761 tcctgtacaa tgtgcagagc taaaaataaa tcatgggata gttggagatg cacatgcagg
     5821 aaactggcat cgtcagataa gcttacttgg aaaagaaagc atagataaaa tgaaaatgaa
     5881 aggctttgac caattaaaat atggagattt tgctgaaaat gtaactacag aagggataga
     5941 agttttttcc ctgcctgttg gtacaaagtt aagaataggg caatgtgaag ttgaagtaac
     6001 tcaaataggg aaaaaatgtc acaatggatg tgaaattaag aaattgacag gtgactgtgt
     6061 gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa ggaattataa atgtgaatga
     6121 ttcaatagat gtaatctaat gaagtcacta ttaaaatata at
//
LOCUS       DAMJZE010000205         6151 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJZE010000205 DAMJZE010000000
VERSION     DAMJZE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.209"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            139..1503
                     /locus_tag="PC541_003418"
     CDS             139..1503
                     /locus_tag="PC541_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2840839.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1642..2808
                     /locus_tag="PC541_003419"
     CDS             1642..2808
                     /locus_tag="PC541_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2840840.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2925..3707
                     /locus_tag="PC541_003420"
     CDS             2925..3707
                     /locus_tag="PC541_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2840841.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3826..4479)
                     /locus_tag="PC541_003421"
     CDS             complement(3826..4479)
                     /locus_tag="PC541_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2840842.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4503..5867)
                     /locus_tag="PC541_003422"
     CDS             complement(4503..5867)
                     /locus_tag="PC541_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2840843.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2318 a          764 c          935 g         2134 t
ORIGIN      
        1 aataaggggc tttttttatt ggaagattta tgttatattg agatgaattt attatgatat
       61 aaaaaataat gttaggataa atttaaatct ttttctaaaa atgtattagt ataattctta
      121 aagaaaggat ttttaacaat ggatataaaa gagttagtta aaatgcaaag aaaatattat
      181 aatacaggta aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta
      241 gttgtatcac aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg
      301 gattttgaag gttttatgac agaggtaggc atgttttata gtgaattaaa ctttgctata
      361 aagaatataa gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca
      421 tctatatcca aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat
      481 tatccatttc aattagcact tataccatta gtttggtcat tagcagcagg gaattgtgtt
      541 atattaaaac cttctgaata ttctgtgtca acatctagtg tagttaaaga catagttgaa
      601 gatacttttt caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag
      661 ttactattgg aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata
      721 atcatgaaat cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt
      781 ccttgtatca ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga
      841 aaactaataa atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat
      901 agaaaaaatg agttaataga gaaaataaaa tattatacta ataagtattt tggggataat
      961 gcatgtaata atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcggatatta
     1021 tctcttattg ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag
     1081 atagagccaa ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata
     1141 tttgggccta tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt
     1201 atacaaatgc caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat
     1261 aaacttttag aaatgattcc agcaggcgga tgttgtatca atgatacagt tactcatata
     1321 gctacgaatt acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga
     1381 aaagctggat tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat
     1441 gtccctatta gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg
     1501 taggttatat tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat
     1561 tttattttta atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta
     1621 tatagaaata aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca
     1681 atttatacat ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt
     1741 gatgtatttt ctaataattt agatgctata tttacaacat tacaaccttt tataatagga
     1801 tttgctattg catatcttct taattttatt ttaaagtttt atgaagacag aatttttata
     1861 aaaagtgaga aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta
     1921 acatatgcaa cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta
     1981 attgagagta ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag
     2041 cttattgata aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt
     2101 gataagtgga atgaatttgt tacatatatt ataaagtttg taacagattt aatcccaata
     2161 ttaggaagta tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt
     2221 gtttcagtgt acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact
     2281 tatgcagtat ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac
     2341 tatacatttg gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta
     2401 acatttgtaa tacttacgtt agttaaaatg ccatatacat tattaatatc agttataatt
     2461 ggtattacaa atattatccc tttctttgga cctttatttg gagctatacc atctacgcta
     2521 atagtgatgt ttgtatctcc tataaaagct ttctggttat tgttgattat attaataata
     2581 caacagatag atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct
     2641 gcattttgga ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg
     2701 gttataggag ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt
     2761 aaattggaca aaaagggatt accgacagat acttccgatt atatgtagct aaaaaatatt
     2821 atgtataatt aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct
     2881 tggtgctttt tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat
     2941 gtaaatttaa atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag
     3001 caaatggaaa agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa
     3061 cattatactt atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg
     3121 tagagcatta tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt
     3181 tttatactga tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta
     3241 aatttgttag agctatattt cctttatcag ataaaaggga agataacttt gtagctggta
     3301 tgtctatggg gggatatgga gctttaaaat tagcgctttc aaaaccaaaa gagttttcgg
     3361 cagttgcaag tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag
     3421 aaaccaatgc agacattcta tttcacgatt ttggtagacc agaagacgct agaaatagtg
     3481 agaatgattt actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa
     3541 tttatcaatt ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat
     3601 atgcagattc tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc
     3661 gtctttggaa tttatggata aagaaattta tagatataat tggatagtac acttatggta
     3721 ttaacaggta tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa
     3781 ttatgatttt aaaatttcag tctctaagaa tttaataaaa ttttattaag ttcttacacg
     3841 agttattatt ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa
     3901 aatctcttcg cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg
     3961 ctttattaca gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc
     4021 aggtttcata gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc
     4081 tttatcatta acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat
     4141 agcttcttca taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa
     4201 acaaaggtca gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt
     4261 atagattaaa gactgcattt catttcttat atcttcatat attatagctc ctatttcgct
     4321 tttacttgag aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg
     4381 cctagttgtt gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat
     4441 actagctttt gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct
     4501 tttcaatctt tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata
     4561 ggaaccaaaa ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata
     4621 acacttacta taacagcata gatagctcct ttttttgtag cacctttcca gcaaagacca
     4681 attacaaatg gtgcaaatat tacacctgta cataatggag ctggccaaaa caataattct
     4741 aataaactat caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca
     4801 atttgagaat actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat
     4861 ttgttataaa tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta
     4921 gacattatgg cagccaatat agcagctaag aataatacag atattaaagg tggcaaagta
     4981 gtcgatatca tttgaggtaa aacctgaaca gatgatatat ttggaaataa actaccacca
     5041 atagctccaa ttattcctat agggaaagca agaaataata gtataaatcc tgcaattaca
     5101 ctacttttaa ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa
     5161 gaaaatattg agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta
     5221 tttcctggaa agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca
     5281 actaatttta agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga
     5341 ataaatgctg atactgcaac tccccacatt cctgatgtat aagtatatat aatgataact
     5401 ataactccta taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca
     5461 aaagaaatca attgactacc cataatagct agtatagata ttggtgttac aagactttga
     5521 taagctctta aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata
     5581 tcacaagata agcttcttaa ttttcctgcg aaaaatgcat ttataaagca agctaaagct
     5641 gcacctatag tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca
     5701 ccaactaagc accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa
     5761 ggcagatttc tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa
     5821 aaaccaatcc atatgtatat tgcacacgta acaattaaaa gtaacatagt cttttctcct
     5881 ttataaaata ttttaataaa atatacaaaa tatatttaaa atatatttta tataaaagta
     5941 gtacgatatt tgttttattg gttttagatt aatatagcta taataattta tgatattaga
     6001 tggaaattac gtaaaaatag aaaattgaaa attctgaaat ttttgcatat tgatatttat
     6061 ctcatagatt cttttttgta attgtaacac gcttttattt ttaaatcaat agtacatgta
     6121 taaaaataaa aggaatttaa ataagaaaaa g
//
LOCUS       DAMJZE010000206         6139 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJZE010000206 DAMJZE010000000
VERSION     DAMJZE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.210"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            173..1498
                     /gene="cdeA"
                     /locus_tag="PC541_003423"
     CDS             173..1498
                     /gene="cdeA"
                     /locus_tag="PC541_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2840844.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1593..2387)
                     /locus_tag="PC541_003424"
     CDS             complement(1593..2387)
                     /locus_tag="PC541_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840845.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2388..3263)
                     /locus_tag="PC541_003425"
     CDS             complement(2388..3263)
                     /locus_tag="PC541_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840846.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3267..3635)
                     /locus_tag="PC541_003426"
     CDS             complement(3267..3635)
                     /locus_tag="PC541_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2840847.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3818..4477)
                     /gene="deoC"
                     /locus_tag="PC541_003427"
     CDS             complement(3818..4477)
                     /gene="deoC"
                     /locus_tag="PC541_003427"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2840848.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4513..5700)
                     /gene="murJ"
                     /locus_tag="PC541_003428"
     CDS             complement(4513..5700)
                     /gene="murJ"
                     /locus_tag="PC541_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2840849.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5777..>6139)
                     /locus_tag="PC541_003429"
     CDS             complement(5777..>6139)
                     /locus_tag="PC541_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2840850.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIR
                     QLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT         2032 a          964 c          679 g         2464 t
ORIGIN      
        1 gaaacagtta gattttacta atataagttt attaattagc attatactaa tttatcttaa
       61 tatgattttt tgttgatttt aagctgttta tcgtataaat aaaatttttc ttgactatat
      121 agttactata tagcttatta tatatagaga gtaataaaag gaggtaaaat ttatggaaaa
      181 tttatttaca agaaaattca ctacttttga atttctaaaa tttgtttctc ctgcaattat
      241 atccatgata tttatatctt tgtacacaat aatagatggc atctttgtat cgacattagt
      301 tggttctgat gctcttgcta gtataaatat tgtactacct ataattaacc ttgtttgtgg
      361 atttggaata atgatggcaa ctggtggagg tgctatcgtt tctatacgta tgggtgaaaa
      421 tagacaggat gaagccaact ctacattttc ttttatagtt ttgttttcat tgattgttgg
      481 gattttattc acagtaatct catatttctt tatcaaagaa atatctattt tgcttggtgc
      541 aacagataag ttattaccat attgtataac ttatggtaaa gttatgattt tatgtacacc
      601 attttatatt ttaaaattta tatttgagta ctttgcaaga actgatggaa attctaaatt
      661 tagtttattt ctatcagtca ttggtggtgt aacaaatata atattggatt atgtatttat
      721 taaatatttt ggaatgggtc ttttaggagc tgcagttgca actgctatag gtattatttt
      781 aacttgtgtt ttaggtatta tttacttctt atctaataaa tctacactaa aattaagaaa
      841 accaaaaacc gattttagac ttataagaga tactatgata aatggttctt ctgaaatggt
      901 tacagaatta tctacaggaa ttacaacatt cttatttaat gtagtagctt taaaattagc
      961 aggagaaaat ggacttgctg ctcttactat agtattgtat gctcattttt taatgacatc
     1021 agtctatcta ggattcgctg ctggagtgtc tccattaata agctataatt ttggtgctga
     1081 aaacagtgat aaattaaaag aaacatttaa acattctcta aaatttatat ttgtttcttc
     1141 tcttttagtg tttatttttg ctttagtatt tgcaccattt attgttaggg tctttgtaag
     1201 tccagataac acagtattta aactagcctt acaaggatta aaaatatttg catttgcttt
     1261 tttgtttgtt ggtataaata tatttgcatc aggatttttt acagcatttc acaatggaaa
     1321 aatttcagct attatatctt ttagtcgtgc ctttgttttt ataatcatag gaatcataat
     1381 tcttcctcct atgttaaaca tgactggatt atggcttaca gttccatttg ctgaggttat
     1441 aaccatattt atatctattc tatttataaa aaaatataaa tgtagatata agtattaaaa
     1501 attaatactt aaaatcaaca tttattaaaa ctaataaacc tgctttaaaa tacaaattta
     1561 caaatttgat ttttaagcag gttttatttt agttataata agtcatttgc atactcttta
     1621 attggtgctt ttctaagtaa taaaattata aatattatgc aaataactat aattcctact
     1681 tttgttccaa ataaaataaa aggattctga aatgaataaa taattttttc taaatacaat
     1741 agcattttat gcatattttt agttgcaaaa ttaactatat aaattatcat taatatcggt
     1801 aatcctatca atatagtaat actagtaact tttctaactc tatatgtaaa agctccaagt
     1861 aacactccta taaaacctat acatatttca tttatacata tttgtacaaa ataatttaaa
     1921 taagttagtg gcatacttga tgttgaagga tctatcagtg ctgatataga tgtccaaatc
     1981 atatctctct ttagtaaaac tgatagttct atactgtaac ctgttataat ttctataaat
     2041 aacttcgata aaaaaatcaa aactatacct aaaatagaaa tcacaaagct taaaataatt
     2101 aaatctatga caatagcttt tatacaactt tttctatctg ctcttatact taaagctcct
     2161 gaaaagtctt tatttgctat atttactcca tacacaaata gcagtatagt tatatatgtt
     2221 aaaataggat tcacaatcgt ggataaatta gttttagatg cttctccaaa aatcttgaga
     2281 ataaacactg gcaaaatatt cataacaatt cccacaatca taaatataat tatatacaat
     2341 ttagtatttt tattaaaaaa ttttatatat ggctttaatt cattcaatta caattcctcc
     2401 ttcttagtca tgttaacaaa catatcttgt agccctatgt atcctacatc aatatttgaa
     2461 tttttaagta ttttttcatc actttcacta aaatcaccat agtaaaaata agcaactgca
     2521 tttccaaatg cctttttagg ttttatattt tttatacatt ctaatttttc taactcttct
     2581 ttatttccag taatataatg agctttttgt tttatgacat caatttcttc atctataata
     2641 atttttccct catttaatat tattacatgc tccagtaaat catctatttc atcaatcaaa
     2701 tgagttgata ttattattgt ccttggattt tttatataac tatctaaaat tatgttataa
     2761 aactcttgtc tatttactgc atccagacca attgttggct catcaaatat ggtgatagct
     2821 gaattagaac aaatacctat tatatttgaa agtatagttt tcatacctct tgacagttgt
     2881 ttatactttt ttttagtatt taaatcaaaa tatttgcata attcttcttc taagccttta
     2941 tcataacttt tataaaaact agaagaaaat tcaaatatct gacctacttt aaaatctgga
     3001 ctataaaatt ctttttccct aacgatacaa acttcttcca aaacttttat atcttctttt
     3061 gggttctttc ctaatatttc tatctcacct tcattttgaa ttaactgatt taccattata
     3121 tttaataatg ttgtttttcc agctccattc ttacctaata aaccatatat cttatttttt
     3181 tcaaagctta gagacatatt attaaaaata ttttgcttct taaaactgtg agataaattt
     3241 tctatcttaa tagaactact catatattaa tcctccttga aattaattat aatatcaacc
     3301 aattcttctc tatttatttc taatttgtta gcttcttgaa gtaggttttt tacaaaatta
     3361 tgtttaaaat tttcttttct agcttctttt ataatagttt ttgcaccatc tgatacaaac
     3421 atccctatcc ccctcttctt atataaaata tttttatcaa ccaatatatt tatacctttt
     3481 aatactgtag ctggattaat tttataaagc tttgaaagct ctgttgttga tggaacctgt
     3541 tcttcttctt ttattccatt tgatagtatc tcatcttcaa ttgctcgtgc tatctgaata
     3601 aatataggtt cttcattatt taatactaat ttcataggtt cctcctttct ccatcagtta
     3661 gttactcaac taattaacta tataacttat aatatcttga aaaatatttt ctgtcaatac
     3721 taaataaaaa acagagggaa aattccctct gttttttgtt ctttatattt ataattaaat
     3781 tttgattttt gtaatcctat cccataagtt aaaaacttta ataatctgtt gtatcttcat
     3841 tttttaatat tttacatatt gagctagttc ctaacctttc tgctcctgcc tctataaatt
     3901 tttctgcatc agaaattgat ttaactcccc ctgcagcttt tatttttaca ttttttccaa
     3961 catgctcttt cataagtttt atatcttcta aagtagctcc acctgttccc attccagtag
     4021 atgtttttat aaaatctgaa cctgacattg ttactatttc acacattttt attttttcat
     4081 cttcatctaa ataacatgtc tctataatga cttttaatac tttatctcct atagcttttt
     4141 ttatttcttt tatttctttt cctatattat cataatcttt attttttata tctgaaatgt
     4201 ttacaaccat atcaacttca tctgctccat tttcaatagc atcttttgct tcaaatactt
     4261 ttgtagcagt agtttgatat cccaaaggga accctatcac tgtacatatc tttattttac
     4321 cttttagata ttctgatgct cttttcacat atgatggtgg tatgcaaact gatgctacac
     4381 tcatatctac agcttcatca cataaaattt taatatcttc ccatgtagtt gtagctttta
     4441 aaatagtatg gtctactgtt tttaatatat gtttcataaa atcctcctaa atttataatt
     4501 aatataatta gattactttt ttaaatgcaa ttttctcttt gcaatgttta gtatcatatc
     4561 aacctcttca actttaaaaa taatcattag tagtgtataa attccacctc caactataac
     4621 tgaaatagct agtgatacaa actcattaaa tgttcctact cccaaaattc caaatataaa
     4681 tttatacgta aagtatgata aaacacccat aattaaagat gcaactaccg atttcaaagt
     4741 tgcttttatt attttatctt gtccaaaata tccaactttt ctctttaggt tcaagaagag
     4801 taacaatata catgcaattg atgatgaact agttgcaaga gcaagaccac catgagccat
     4861 tggttttatt aaaactaagt ccaatactat atttacccct acagatataa tcccatttac
     4921 cataggtgtt tttgtgtctt gtaatgaata aaaaaccttt cccaaaatat ctcttaagcc
     4981 aaatgcagtc ataccaactg cataaaaaat taaggcagtt gctgtcattt gggttgccct
     5041 cgcatcaaaa gcacctctct caaaaatgat tctaacaaca ggagttgcaa aaaaaataga
     5101 agcaactgaa ataggaatca tagaaattat tatcatattt atactggatt taactgaact
     5161 agtaaacttc ttttgattat tttctgcact taacttagat aacataggat acataacaga
     5221 tactattgaa gctgtaaatg tccctgtaac aaaaccattt aatctatctg cataagtaag
     5281 tgctggaatg cttcctttaa ctaatgttga ggctaaagtt gtatctacca atgagttcac
     5341 ttgattaaca gcaacaccta taaatactgg tgaaagtaat gctagtaatt taataaggct
     5401 atcatcttta aagtttaagt agtataagta tttgtagttc gtttttttaa caaaaaacat
     5461 gtaaaagagc agttgaacta ccatagcaac cacagcacca attggaagta tgtatggtcc
     5521 aaatatggta cttaacatta ttgaaataat tattactata ttatagggta tacttccaaa
     5581 tccaactaca ataaagttct ctttaatttg taaaaatgct gacataacac tcgtaatacc
     5641 tatgaatatt attcctgttc tgaacctccc atgactaaag tcacagggtt cctgcttcat
     5701 agaattttgc atactaaaat atgtcttact aattctccac aggctatccc cgtagttcct
     5761 acggttctta catacgttat ttattttgaa ttgttattag tcttaatcct tcttttaata
     5821 tatttatact tgcatttata tctctatcgt gagtttcatt acaactagga caaatccatt
     5881 ctcttatatt taagttctta acttcctcgt ttttataccc acatttattg catatttgtg
     5941 aacttgcaaa gaatttacct acctttacaa tttgtctacc ataccaatta gccttgtatt
     6001 ctaattgacg aataaactct gaccatgata catcagaaat taagcgagat agtttacgat
     6061 ttctaatcat attctttact tgtaaatctt ctatgcaaat aatatcgttc tcttttatta
     6121 atttagtaga tagtttttg
//
LOCUS       DAMJZE010000207         6137 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJZE010000207 DAMJZE010000000
VERSION     DAMJZE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.211"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            79..1560
                     /locus_tag="PC541_003430"
     CDS             79..1560
                     /locus_tag="PC541_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2840851.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1593..5027
                     /locus_tag="PC541_003431"
     CDS             1593..5027
                     /locus_tag="PC541_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2840852.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5045..5791
                     /locus_tag="PC541_003432"
     CDS             5045..5791
                     /locus_tag="PC541_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2840853.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5740..6048)
                     /locus_tag="PC541_003433"
     CDS             complement(5740..6048)
                     /locus_tag="PC541_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2840854.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2561 a          605 c          932 g         2039 t
ORIGIN      
        1 tgtaaaaaat acttattaat agtaaaataa agtgaatcaa ttatattata attgctaaag
       61 agagtgaggt gtagctattt gaaaaaaaat agattaatag caaagaatat atcaatagca
      121 tttatagtac tattcttttt tagtgtattt acattttttt atgtaggtaa tattaataga
      181 gtgttagaat atgaaacaaa tgacataata acagttacaa ttgctggatg gattatactt
      241 tcatttttat ttttaggtat tataatttac atattatatt caaaagcaaa tagtcaaaaa
      301 actatagaaa aagtagcata tacggatttt gtaacaggtt attccaactg gagaaagttt
      361 gaattagatg ttacaaattt attaaagaaa acatcacaaa ataataaata tgcaatggtc
      421 atattcgata ttgataaatt taaagcaatt aatgatatat atggccataa aaagggaaat
      481 ttaatattaa aagatattgc agatacttta aatgaattga ctgatatcaa tgaaactttt
      541 gctcgtgtaa gtgctgataa tttcaatata ttattaacat ataacaaaaa agaggatata
      601 ataaatatta tcaaaaagat tatggcaaat aatgaacttg taaacttatc ttttggaatt
      661 tatgaaatca aagataagga tttatctgta agtgtatata gtgatagagc ttcacttgca
      721 aaatcatcaa taaaaaataa tagtgatgta aattttgcat tttttaatga caaattaaga
      781 gagaaattat tatttgaaga taagatagaa aaagaaatgg aatatgctct tgaaagtggg
      841 caattcgtaa tgtatttaca accaaaatac aatattaaat tagataaatt ttgtggaagt
      901 gaagccctag tcagatggca atatacagaa aaagaggtca tatatccagg tgattttatt
      961 cctatatttg aaaaaaatgg atttattaga aagattgata tgtacatatt agaacaagct
     1021 tgtaaagaaa tccggtcttt atttgataaa ggaatttcac ctttacctat atctgtaaac
     1081 ttctcaagag ttgatttttt taaaaaagat tttattgaaa acatagtaaa tatatgtgat
     1141 agatataaaa taccatatag tttaatagaa attgaaatta ctgaatctag tatgtttgga
     1201 gatactgata ctctatttaa tgttagtaga aacctacagg atattggctt tatagttgca
     1261 atggatgatt ttggctctgg atattcttct gtaaatatgc ttaaaaatat accattaaat
     1321 gtaataaaat tagatagagg attttttgtt gatgataaag atgttgataa gagtcaaatt
     1381 gttataaaaa gcatagtttc tctcattaag caattaggaa taagggttgt tgctgaagga
     1441 attgaaacaa gaagtcaaat agaaatgtta aaaaaagcaa attgtgatat tgttcaagga
     1501 tattattttt caaaaccatt accaattaaa gaatttgaaa aattagtcta taaaatatag
     1561 tcagcgagtc ttaaatggga ggtggtgatt aagtgataag agcaaaatcg ttaaaaaatg
     1621 tactgagtat tgttttaata tttttattta caataatttc tttttctgat tttgcattta
     1681 aaactaatgt ccaggaagaa gaaaatagta aaattaattt aagagaaata tccctacaaa
     1741 gttccaatgc tataaaaata aaattagata gttctctatc ctttattaaa agtgtggcaa
     1801 atatggtttc ttattttaat actatagata actcaaaaat agttccatta ttagctaata
     1861 tatcaaaaga atctgacttt caaagaatct ctattgtatc tcctgatgga ataagtcata
     1921 ccagtgatgg caaaattatc aatgtgtcaa agtatcagta ttttaagaaa gctattaaag
     1981 gagaaagtaa tatatctgaa gttgtgaaat ctccaattga tggaaaagat acttttgtta
     2041 tagctactcc tgtatttaat aagaataaca aaataattgg tgttttatac ggttcttact
     2101 acaatgataa attagctgaa tttatagata taacaagttt taatagtgaa gggtacatag
     2161 atatatttga atctagtggt caatttgttt taaaatcttc acatacaaac aaactgatta
     2221 agtcaagtag taacatatat gatatattta aaaaagcttc ttttgatgat ggttatagct
     2281 ataaaaaaat atatgaagat acaaaaaata acaagagtgg ctttacctca tatgaataca
     2341 aaggagaaaa tcgttatgct acttatacac ccattggtgt aaatgactgg tatgtaattt
     2401 ctgtgatacc acaatatact gtaagaagaa acattgataa aataaatcaa ttagcatttt
     2461 ctcttatttt caaggtattc ttattgttta ttatgcttat aatatatatt atttataaaa
     2521 acaataaatc tcagaaagat atactaaaag ctaacaaaga aatctcatct attacaaata
     2581 gcattcctgg aggagtacaa aagtgtaccc tgggtgaaac atgcaatatt aattttttaa
     2641 gtgatggatt tatagagttg tttggttata caagagaaga aataaaaaca ttgtttaata
     2701 atgattttta tagtttaata cataaagatg atgttgaaag aataaaaaaa gaaattttta
     2761 aacaacttta caaaaataaa actgttgaat tagaatatag gattataaca aaaaatggta
     2821 atacagtatg ggttttagat aaaggggagc ttataacaga acctgatgga aaagaatatt
     2881 tttgttgtgt aactgtagat attactagaa caaaacaatt agaacaagag atggaacaaa
     2941 aaaatctaga acttgaaatg atatcagaaa atgttttagg tggtataatt ataacagaat
     3001 ttaatgatga ttttactata aaatacgcta actctggtta tctttcaatg attggatata
     3061 cacgtgaaca attagaaaat gaacttaaat acaaagcttg taatcttgtt ttttatgagg
     3121 ataaaaagaa ggctcttgaa agtatacaac atcaattaaa cacaaaagga atagctgtat
     3181 atcaacatag aataagaaga agaaatggta atattatttg ggtttctata aaaggaaggc
     3241 ttattgttga tgatggtatt gaaaaaggta tttggattct aaatgatata acagatttaa
     3301 aaaatgcaga acaaaaactt aaaataaatg aagaaagatt tagaattgca ttaaagcaaa
     3361 catcaaacac tatatttgag tatgatataa aaacaaagtc acttttaaat actagtaaag
     3421 agcttgataa atataatatt tataaaatta ctaaaaacgt tccatattct ctagtagaat
     3481 caggagttat tcataaagat tttagtgatg attttataaa catgtatgaa aatgtaataa
     3541 atggagcaaa aaaagcaagt tgtgtagtta agatgaaact tccagatgaa aattatatat
     3601 ggaataaagt taccttgact actatttttg atgaagatgg taatcctgta agagcaattg
     3661 gtatacagga agatataagc aaacaaaagg aagcagagat aagatacaat caagaaggta
     3721 agtatcattc agctatattg tcggaagcaa tgataactta tgaaataaat gtatcaaagg
     3781 atacttttat caaaggaaat gaaagttggc aaaaaccatt tggaattaag cctgacaata
     3841 actattctca aatggtaaaa actatagcaa atagtattgt acataaagac aataaagaaa
     3901 catttttaaa tacattttta tgtgaaaact tattggatgc atataaaaat aatgtgactg
     3961 aaataaaatt agaatatcga agaatgaatc aagaaaataa aatgatttgg gtcatttgta
     4021 caatgcactt aataaaagat tctattacga acgatataaa agcacttgct tatgttaaag
     4081 atattgacaa gcaaaaaaat gaagaaatta gattaaaatt aaaagctgaa agagatactc
     4141 ttacaggatt atataataaa ggtactacag aaaaattgat taatgatttc ttgaactctg
     4201 aggaagcaag cttaggaatg catgcatttt taattgttga tatagataat tttaaatctg
     4261 tgaatgataa tttaggacat gtatttggag ataaagtatt atttgaagtt tctaaaaacc
     4321 taaaacctat atttagaaaa gatgatatag taggtagaat tggtggagat gaatttatag
     4381 tattcttaaa aaatattcag tcttttgaga atatcaaaaa gaaagcagaa aaaatatgcg
     4441 atatatttag aaattcttat acagaaaata aaaagacata taaaatatct ggaacagttg
     4501 gaatttcatt tgtaccagaa catggaactg tatttgaaga actttataaa aaagcagaca
     4561 tagcacttta ttatgcaaag agcaaaggaa aagataagta tgcactttat aatgattgta
     4621 tctaccaaaa tgaacttgaa aataatataa gtgataataa tataaaatta gcagataaat
     4681 gtgagaaatc caaaccagta cttaaagaaa ttcttgattc tattgataat tatatctata
     4741 tagtgaatcc aaatacttat gaacttcttt ttgtcaataa taaggtgtct cgtttggatt
     4801 tagagataaa attaggagaa acctgttata aatcactatt aaaatctgat agaccatgta
     4861 ataattgccc tcttaacgga ttagaagaaa ataagcattc aaaattcaaa atagagacaa
     4921 atacatttgg taaaaatatc agtatagcaa gttggattaa ttgcatagat ggagaataca
     4981 actgtcttat agagtgtata gattcttctt tatacaaaaa taaataaaaa aggactatga
     5041 agatatgtta aatagtataa ctttgatact tggtataata tttacaattt actattttta
     5101 tctcaaaata gtttttggag gaatttcatt tagtgagatt ttcttagttg taggaattgt
     5161 actaattatc tatcaaatat ttaagaaaaa aattaaagaa aagaaggttt tttatagggt
     5221 gttaaaaata ataatcagca tttttttaat agtatttgta gttactgaga gtctaattat
     5281 tttttatccc aaaaatagtt tagaatcaaa aagtgattat ctcttaattt taggagcttc
     5341 tgtaaaaaag actacaccaa gtacaaccct gaagggtagg ttagacactg cattgaaata
     5401 cctaaaaatt aatgataatt gttatgtggt agtatctggt ggtaaaggaa gtggagaaaa
     5461 aataactgag gcaaaagcta tgaaagatta tcttataaaa aatggtatag ataaaaatag
     5521 gataatagaa gaggataaat ccacgaatac ttatgagaat tttaaatatt caaaattgaa
     5581 gatagaagaa catagtcaag gaaaactaag cgatttaaaa gtaaaaatag taacaacaga
     5641 ttttcatgtt ctcagaagta aaatattagc atatagaaat ggatataaaa atacgtcttt
     5701 ttatgctagt aaatcaaaac tttcatttgt accaacttac tatacaagag aattttttgc
     5761 attatggaaa acaatcattt ttgatagata ataaataaaa agactagata taaactcata
     5821 tctagtcttt tctattccct taattctaat ccattttcca actttacttg ttgtagaaat
     5881 ttcaacattg ggatttacaa gtaattgctt ataagaagtt ttatatttta cgattccaaa
     5941 atataatttt ccttcataat ccattataaa tccgaatggt cttactcttg gtttattatc
     6001 ttcaactgta gcaaaataga atgttgagtt ttctttaaaa aaattcatta ttttttcatg
     6061 attaaatcct ctttgtatct tatactaata taatgaatat aaataaaata ttcataatac
     6121 taatataaat cgatata
//
LOCUS       DAMJZE010000208         6028 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJZE010000208 DAMJZE010000000
VERSION     DAMJZE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.212"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            92..712
                     /locus_tag="PC541_003434"
     CDS             92..712
                     /locus_tag="PC541_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2840855.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(878..1414)
                     /locus_tag="PC541_003435"
     CDS             complement(878..1414)
                     /locus_tag="PC541_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2840856.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1527..2201)
                     /locus_tag="PC541_003436"
     CDS             complement(1527..2201)
                     /locus_tag="PC541_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2840857.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2404..4278
                     /gene="asnB"
                     /locus_tag="PC541_003437"
     CDS             2404..4278
                     /gene="asnB"
                     /locus_tag="PC541_003437"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2840858.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4370..5806)
                     /locus_tag="PC541_003438"
     CDS             complement(4370..5806)
                     /locus_tag="PC541_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2840859.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         2319 a          702 c          883 g         2124 t
ORIGIN      
        1 ttttaatatt agattcattt tttgagattg gtatataata aacatataga gtatataata
       61 atagttaaag ttaaaaaagg ggaaaagcaa tatgcagtat atacaagaac tacttgattt
      121 gactcaaaat tattgggtgt tagccacaat aatcggctta ttaagtgcat ttatagaaag
      181 ctttatacca gccttaccac ttgttgctat agttacagca aatgctgcaa tacaaggatt
      241 atttattgga tgtttgttat catggattgg ctcaggactt gggacaactt cattattctt
      301 attaataagc agatttaatg atagtaagct ttttaataag ttgagaaata gtaaaactga
      361 aaaagcaata agttggatgg ataaacaggg atttaagtta ttatttatag cgtatgcctg
      421 tccatttatg cccggttgtt tagttactat cgcatcagct tttggtaaaa aagatttaaa
      481 agattttgtg ccagcaatgt tggcaggaaa atttgtaatg tttatagtta taagttacgt
      541 ggcaagtgat atagaaggtt ttataacaag tcctttaaag atagcatcat ttatattatt
      601 ggtgtttttg tcatggaaga ttggaaatag agtaaataaa aatcttgaaa atcataatta
      661 tgatttccat cataaaaaac atcacaatga taaagatgat aaaatgattt agcttgtacg
      721 aaataaaacg tatcttaaaa tgatttagta ctaaattatt ttaagatacg ttttattttt
      781 tgtattaagg tacaaaattt ttagttcttt tgtaaactaa aatatataaa acttatttat
      841 acttagttca aaatattgtt atttattttt tctacaacta taataaaata taaaattgat
      901 atccaatcaa aaaaataact atgaaattta atagcttaga tatgaatttt tctgtttttg
      961 agttagtttt tataggtagt aaagaaatgt ttatggtaat gggataaaac aattcaatac
     1021 cttctttagt aataaggtca agacacaagt gagatagata tcctattata aacccactgg
     1081 acaaacatga gaaaactata ttattgttag tgaagattgt cagagattta aaaaaactat
     1141 aaataagtaa taaggcaatt agactgtgag taaaccctct atgtctaaac ctacttccaa
     1201 ataatttgta tattaacata aattttttac ttatgtaaga acctctcata tctatatctg
     1261 gaaataaaga acctaaatat gcaaaataaa tataaattat aattaatact attttatatg
     1321 caatatcgta tttaactagg tatagattgt ttataaaggg gagtatcaaa agtgcgaata
     1381 tataacctcc cctttcatgt gtttctttta tcatataaaa atcctcctga atcgtcacta
     1441 taaacattat attggtaaat ttagactatc ttttattata ttatatagca aacataagtt
     1501 tttttcaaaa ggattttaat atttaattaa tcttgagatt ttaaattttt gtaattttct
     1561 aaagcaccat tccaatttac tacattaaac cagttgtcaa tgtattcatt tcttctattt
     1621 tgatatttta aatagtaagc atgctcccaa acatcaagtc ctattatagg agttaggttt
     1681 ttacttacag ggctatcctg atttggagta gtcataatag ataatttgcc atctttagta
     1741 gctacaagcc aagcccaacc agaaccaaag acatctaaag cagatttttg gaactcttgc
     1801 ttaaattttt caaaagaacc aaagtctcta tcaatagctt cttttaaaga ttcagaaggt
     1861 atggtttttt ctggcgtcat tatatcaaaa aagaatttat gattataagc tccacctgca
     1921 ttatttctta cagttgtagc aatatcttta ggtaaagaat ctaaattttg caataattca
     1981 cataaagaat aattataaag ctcaggatat ttttcaagag cagcatttaa tttatcaaca
     2041 taagcttgat aatgcttatc atgatgcagt ttcattgttt ctttatctat ataaggttca
     2101 agtgcatcat atgcataagg taatggttta accttaaatt tgttattttc aggtgtaaaa
     2161 gcaaatgaag ttatgcactg tgagattata aataaagaca taataactgg tattaatatt
     2221 tttttcttca taaaaatcac ctcctaatat agtatttcta gaaataaaaa aaatatgaaa
     2281 tattaacaga taatttataa gaatttaata gcaatttcat tttaaattga ataagtttta
     2341 aagccaaaga caaaactatg aaatatagaa taagactaaa ggacagaaag gaattttgat
     2401 tatatggacg gcaatatttc aatttattat aaaggtgata tatctaaaaa agaagatatt
     2461 aaatttaaaa taaaagaatc tgtaaataat acaagacttt ctagtagaat agagtataag
     2521 gatgaggaaa aaacttttga ttttgaaaga tttaatgtaa ttgatgctat ttatggaaat
     2581 aaaccatttg aaaaagatgg aaaagttatt gtttttaatg gaaatatata taactatcat
     2641 gaaataaaag aagaattaat taaaaaagga cacaatttta ctacaaatga tgatattgaa
     2701 gtcttattag catcttatat agaatttggt aagaattgtg tacataagat taaaggaatg
     2761 ttcaacttta taatctatga tagagaaaat gaaagtatat ttggagcaag agatttattt
     2821 gggataaaac cactttatta tataaataag gaaaatgcaa taattttttc ctcagagtat
     2881 aagtttttac ttgaatatat gaaaaattta aatataaatg aaagaagctt acaaagttat
     2941 ttttcatttc aatatgtact tcctgaagat actatgatac aaggaataag gctgatacca
     3001 gcaggtcact attttagggt agaaaatggt atactatctt taaagagata taataaactt
     3061 gagtttagaa gtagtacaaa atttttttat actaaaaatc acttaggcaa tagagatgtg
     3121 aatgaggatg atataagaaa tatagtagta gattcaatag taactcatat ggaagaggag
     3181 aaagaaattg gaacattttt gtctggtgga atagattcat ctattataac tactgtggct
     3241 tcacaaatta atccaaatat aaaatctttt tctgcaggct ttagtgtcaa aggatatagt
     3301 gaattagaag tagcaaagaa aacagctgat aagttaggta tagaaaatat ccagattaat
     3361 ataacacaag atgaatatat aaaatcatta ccgaatgtaa tttattcttt ggatgaccct
     3421 atagcagacc cttcagaagt aggtctttat ttcttaatta aagaggcagg taaacatgtt
     3481 aaagtagcat tgtctggaga aggagcagat gaattatttg gaggatataa tatatataaa
     3541 gagtatagta ctatgaaatc agttgtcaat tcacctactt atattaaaag tatattagga
     3601 agagtttcag aacttatgcc aaacataaaa ggaagaaatt atctttatag agctacaaca
     3661 ccattagaaa aaagatatat aggaaatgca aaagtttttg aaaacagcga agtaaaaaga
     3721 ttctttttta agtataagga aaagaatata tatgaatacc tgttgtctaa tttgtataga
     3781 gatgcacaaa aaaataatta cgattatata agtaaaatgc aacatataga tgtgaataca
     3841 tggcttcaag gcgatatact tcaaaaaatt agcaaacttt ctacagctga gcaggttgaa
     3901 cttagagtac catttttata taaagatgta tttgatgtcg caaaaaattt aagaatggag
     3961 caaaaaataa ataagaataa tacaaaagtt ttacttagag aggcgtttag agaaatagtc
     4021 ccagaacatg ttgttcaaag gaaaaaatta ggatttccaa ctccaataag agtttggtta
     4081 aaagattctc taggggatat tgtaaaggaa actatttata attcaaatgt agatgaattt
     4141 atagataaaa aatatgcaat aaaactttta gatacgcatc ttaaaggtca tagagataat
     4201 tcgagaaaaa tatggactat atttacattt tgtctatggc atcaattatt tattgagcat
     4261 aaaaatgtcg agtattaata cttttttcaa tattaatcag agggcaaaag catgcaatgt
     4321 tttcaccctc ttgattatta tggaatagga taagtagaat tttgattgat tagtttacta
     4381 tctcaataaa attattggca aatattttga caagactact ttcaacagct aaatttaagt
     4441 tttgtccatc atcatatcca gcggaaatca atcctagtaa gtttccaaac atatctaaaa
     4501 gagctcctcc tgaactacca ctagagattg gagctgtaaa ttgtatcatt tgaaccgttt
     4561 caaaatcacg aaaagctgaa ataattccat ctgaaactgt attaaaaaga cctagagggc
     4621 ttccaattgc aactattttt tgacctctca caggtttttt actgacttta acaggtattg
     4681 gcttgcattt tctatcaacc tttatcacag ctaaatcata atctgaatgg tattttataa
     4741 tttggtaaga tgtataaact ttatcatcat tttcaaatct aactaaaaat gaatacccat
     4801 cattgactac atgaagattc gtaagtatat aaccttcatt atttattact actccagaac
     4861 cgctcttaaa cggtttattt tcgttattgc acacaacaat cataacaaca gaagctgata
     4921 actttgcaag gtcttcataa gaaagttcta cttcctcatc ttgtacaatt tttttagatg
     4981 ttagatttgt gttattaagt gtatttttag agttaggttt attcgtagta ggagaagtct
     5041 tattggtatt ttcactgtaa tttatatttg taatatgcct ttcaaaaggt atttctaata
     5101 tatttggagt cttcctagaa agttcaaata gagtagaaaa ttttgaacat tcaggatata
     5161 aatcaagaat atctttattt ggatttatat ataaaatatc acacccaagt tgtgataaaa
     5221 aatataagaa aaaaagttct tgccttttaa tatttccaaa gtatactatc tttggtgaaa
     5281 tagtagtctt caccttgtta taaaataatt taggtagaaa atagtctatc caacacaaaa
     5341 gtttaactgc aaagtttttg atgatagagg atgtacaatt tggcatactt gtggaaaata
     5401 aatctaaagt atttttaaat gcaatctttt tagtccaatt taaggtgtca ttttctattt
     5461 tacatggaaa gtcgatatta tataaatccc aactgttaaa cttagaacta ttataaatat
     5521 taaaaatttt aagcatctta tctgtttgtt ctttgtcaaa atttattttt agattgttta
     5581 atctaatata gggaattgta ggattattaa aaaaataagt atctatattt ttaatttttt
     5641 ctatgtattt atcaattgaa tcatcaacac ctatatatct tgtaaagtag acttctttaa
     5701 tatatgaaga actcttattt ttataggtag tatcagatat aaattcaatt agaggttcag
     5761 tagacttttt tattgaaatc tttaatggtt taatagtaca aatcatattt tttctcctat
     5821 aataagatga tataatctat tataaaacaa cttaaaacaa tatagtacaa taattttgta
     5881 gtttcaaaat aaaatatgta cttgtttata taaatgatat ataattacaa taaaattgcc
     5941 gaaaagccct ctcctttagg tgtagggatg aaaggtaagg tcttaatata aaaaaagtta
     6001 gaataatgct tgtaatctaa taccgcta
//
LOCUS       DAMJZE010000209         5994 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJZE010000209 DAMJZE010000000
VERSION     DAMJZE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.213"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..410
                     /locus_tag="PC541_003439"
     CDS             <1..410
                     /locus_tag="PC541_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HDF2840860.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /locus_tag="PC541_003440"
     CDS             complement(710..1297)
                     /locus_tag="PC541_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2840861.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="PC541_003441"
     CDS             complement(1383..1820)
                     /locus_tag="PC541_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2840862.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="PC541_003442"
     CDS             1924..2922
                     /locus_tag="PC541_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2840863.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="PC541_003443"
     CDS             complement(3042..3968)
                     /locus_tag="PC541_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2840864.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4353..5957
                     /locus_tag="PC541_003444"
     CDS             4353..5957
                     /locus_tag="PC541_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2840865.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         2228 a          755 c          940 g         2071 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttaaag
      481 atgagatttt atgtgagtgt gtaaaagtta aagatagatt taagtaggtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atagaattat tgccttaatt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttctaat aaaagtaaaa
     2041 tattcttctc taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttgtgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaacctccta aaaaatattt aaattttttg cattgaatag attacatgag
     4021 atgttatttg agaactaaga tacttatatt gtgaataata atgtaactgt atatttaaaa
     4081 taatacatca tgatttaaaa aattacaatt gtacagatat aattgagttt agatatttaa
     4141 atttaacaac ttataatatt ttttcaatta gtataaagcc tattaatttg tcttatttac
     4201 aatgaatagt atttacttct tggcaaacaa taattaaaaa taaaactttt atatgattaa
     4261 actctaaaga aaaagtttat aaagaagata atataattaa gcagaaaata atgaaaaaaa
     4321 tttattaaag tagacaacgt aaggagaatt ttatgaaaga aaaaatttat tacaatggaa
     4381 atatcatcac aatggaagat tcaatatgtg gagatgctat tcttataaaa gacaaaataa
     4441 taaaaaagat aggaacaaaa gaagaagtat ttgctttgaa aaataaagat acagaaatta
     4501 tagatttaca aggtaagaca ttgatgccat cttttataga ttcacatagt cacttgatag
     4561 cttttgctac tactctaaag ctagttcctt tagaagatgc aaccagtttt aaagacatag
     4621 taaaaaaaat acaggatttc aaagagtcga ataatataaa aaaaggagac tggataattg
     4681 gattttcata tgataataat tttttagaag aaaataagca tccagataag agtgtactcg
     4741 atagtgcatc ttcagaaaat ccaatactaa tttcacatgc ttctggtcat atgggagttg
     4801 caaatacatt ggggttaaaa cagcttggtg ttaccaatga aacacgtgac ccagaaggtg
     4861 gacatatagg tagagtagaa ggcagtgaag aaccaaatgg atatttagaa gaaaatgcat
     4921 tttttaatgt agcatctaaa ataaaacaac catcatcaaa tgagattttt aattcaattg
     4981 aaaaagcaca gaacatatat ctaagctatg gtattacaac agctcaagaa ggtttaatgg
     5041 aagaaaatca atttaacatt ttaaaagcaa tggcaaatca aaataaatta aaaatggatg
     5101 ttgtaggata tgtaaatttg aaaaaaagta agtctgtagc agataataat agggagttta
     5161 taaaaaaata tataaataga tttaaaatag gaggttataa gattttctta gatggttctc
     5221 cacaaggtaa gacagcatgg ttgagtagac catatgaaaa ttcagatgat ggttattgtg
     5281 gatatcctat atacaaagat gaagaagtag aaaagtttat agatatttca ctaaaagaaa
     5341 agatgcaact cttaactcat tgtaatggag atgcagcagc agaccagttg atagatgcgt
     5401 ttgagaaagt tttaaattta aaagagcaaa gtagtgaaaa tcatataaga ccagttatga
     5461 tacatgctca aacagtaaga gaagaccaga ttgatgatat gaaagtaata aatatgatac
     5521 cttcatactt tgtagctcac acatattatt ggggtgatat tcatatcaaa aaccttggag
     5581 aagatagagc gtttaagatt agcccactta aaacaactat agaaaaggga ttaatttaca
     5641 ctcttcatca ggatacacca gtaatagcac ctaatatgct ggaaacagta tggtgtgctg
     5701 tcaatagaat tacaaaaaaa ggtatacaaa taggagaaaa tgaaaagata agcccattag
     5761 atgctttaaa gggtgttact ataaatgcag catatcaata ttttgaagaa gataaaaaag
     5821 gttcaattaa agaagggaaa ttagctaatt tgataatctt agatgaaaac ccacttacaa
     5881 tcgaccctat gaaaataaaa gatattaaag tattgcagac tataagagaa ggagaagtat
     5941 tgtattcttt gaagtagatg aaagttagat attttataat ctataaaaaa tagt
//
LOCUS       DAMJZE010000210         5986 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJZE010000210 DAMJZE010000000
VERSION     DAMJZE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.214"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(954..1505)
                     /locus_tag="PC541_003445"
     CDS             complement(954..1505)
                     /locus_tag="PC541_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840866.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1696..2658
                     /locus_tag="PC541_003446"
     CDS             1696..2658
                     /locus_tag="PC541_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2840867.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2953..4797
                     /locus_tag="PC541_003447"
     CDS             2953..4797
                     /locus_tag="PC541_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2840868.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5119..5703
                     /locus_tag="PC541_003448"
     CDS             5119..5703
                     /locus_tag="PC541_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2840869.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2337 a          588 c          923 g         2138 t
ORIGIN      
        1 cctttgaaaa taaaaattaa aaaatattta atgctactac aataggaact agaactacac
       61 ttaataaata tacagaaatt agatttggct caatataaaa tacaaatata gaatttaggt
      121 gttttttatg aaaaaatttt tatatgcttt atatagtttc attgttatta tagctaattt
      181 tagattaaaa gaaaaaaatt ataactttat tttattagca atctttttta tattgctgtt
      241 aattcaaaat ggagttttaa aaacaggtta tcaaaataga gctacatttt atcaagataa
      301 ttattttaat agagaggata aaaatgataa gataaaattt atgactataa agatttttat
      361 atttttgtca ttaccattat gttttaatat actattcaat tatttaacct cttaaattat
      421 atttagatat aggagtctta tgataaaagt aagcttatgt gttatttgta gtaaatataa
      481 agtgcatata gtgataaaac aaaagtttca tataaatgaa gaaaaaaata taaaataatt
      541 tatttaacta cttaaaaata aactaaaagg gataataaaa tagcttttag gttatggtaa
      601 taaaggtatt taaaaagagt gcataagcac tccaattgtg agaaatacat acacaaatca
      661 ttattacaac aatgaaaaat gtacatataa gagacgtttt gtatttgttt taagtgataa
      721 tattttaata ctaactaaat aattatcaca atagtaaaaa agaaaaagga gtattttgta
      781 ctccatttgt caaaaatata aaacttttat ataagcttat tataacataa ttgataaaag
      841 tgtaaagata tataatgaat ttataaagaa aaataaaata aaaaagataa atcctaatca
      901 aaggatttat ctttttgtat agaaaataat tattctatta ttaaatggca tggctaaact
      961 ggataataat ttattattac tgctttcaat gttttttctc cttgattatg gtatgtatga
     1021 attgattcag ctgaaaaaca aatagagtca ttttctctaa gttgataaat agagtcattt
     1081 acttctaatt ttaattgacc ttctataatc ataatatatt cctgtgattt ttcagaatga
     1141 ccaacagatg tataactatg accttcttct aattccattt gaaataattc gaagtttcta
     1201 gttggtgtat tgggataata acaataaaga cgataatctt tattttctga aacttgtaca
     1261 tctatatcag tttttgatac aataaaagtt tcattttgag gttgttccag tattgcagta
     1321 tatggaacat ttagtccact tgcaattttc caaattgtgt ttactgttgg gtttgagtct
     1381 cccttttcaa tttgcgaaag cattacttta cttacgccag ataattcagc aagttgacct
     1441 agacttaagt tacgttcatt acgtaatcta tttaaatttt tagcaataac agtattaata
     1501 ctcatagtag ctccttaaaa tgtaaattat tgacaaatat aatttacgaa tgtataattt
     1561 atttaacatt atttataatt tacaaatgta taatataatg aacgttaaat acactgtaca
     1621 tccgtaatat aaattatact gcataaagag ttgtttttca acggatgata gttgagagat
     1681 taagggggaa ttgtaatgcg tgtacttaat aatgttacag aggctatagg caatactcat
     1741 atgttacatt tatccaaaat agcagatgaa tttggagttc aaggtaatat ttatgcaaag
     1801 atggaatact taaatccggg atttagtaaa aaggaccgtg tagcattaca aatgattacc
     1861 gaagcagaaa atgcaggttt attagttcca ggtcaagcag ttgtggaatt aacaagtggt
     1921 aatactggaa caggactagc aatagcttgt gcatgtaaag gatataaatt tatagcgtgt
     1981 atgtcaaaag gaaattctat ggaaagagca agaatgatga aagctttagg tgctgaagta
     2041 gttcttgtag accaaatgcc taattcagtt catggacagg tatctggtga agatttggca
     2101 cttgtagaaa ttgaggctca aaaaatagca aaagaaagaa atgcatttag agcagaccaa
     2161 tttaatttag aagcttgtaa tcatgcacat gaaaagtata cagcagaaga aatgtgggaa
     2221 caattggatg gaaatattga tgtatttgta gattttcttg gaagtggtag tacttttgca
     2281 ggatgttcaa aaacacttaa aaaacataat tcttctatca aatgttatgt tgttgaacct
     2341 gaaacagcag caatttattc aggtaaagaa ataactgacc aagggcataa aatacaaggt
     2401 ggaggatatt ctatggattt gccattagta gacaagaaac ttattgatgg ttatattcaa
     2461 gttacagacg aggaggcaac tgatgtagca agaaaattag ctaaattaga aggtgtattt
     2521 gcaggatttt cttcaggggc aaatgtatgt gcagcaatta agttgttaaa gagcagtgaa
     2581 aaaggaaaaa atatagtttt aactcttaat gatagtggtt taaaatattt aagtactgat
     2641 ttatatgaag aattgtaata atagcacaat agttatgcaa tgttttttaa attaagtata
     2701 tagatttaaa ttataaaaag tatctcaatg taatttttga aagttataaa ggagatactt
     2761 ttttattttg taaatatgga gtatcctact tataagtatt aagctatata gatattgatg
     2821 attagctatg caataaaaaa gagtatgtgt ttttgcattt atacatcata acttggaata
     2881 atacatatta atgttctaaa tgtatttaaa acatatgata aaattagaac aaatagatac
     2941 aaggagataa aaatgcaaaa gagaatttta agaaatttta tatttacggg taaaaatatt
     3001 ttaagtaaac ttgtttttat aataaaatat aattttagta cattaatttt atttgaatta
     3061 tttcataaag gattagcctt attttttata tggccatcta ttagatatat atttgatatt
     3121 ttaataaaaa gtttacatat agtctaccta aatatggata attttataaa agttttgacc
     3181 aatcccatgt caatagttct gataatacta tcagttataa tactagcttt ttatgcattt
     3241 tttgaattta cctctgtaat tatttgtttt aataaatcga taaaatatga aaaaataggg
     3301 ttatttgaac tctttaaaat atcatttaag aactctataa aattgttata tcctaaaaat
     3361 atactattat ttatatttgt gatattaata attccattag taaatacagt tttaatttca
     3421 gggtttatag gaaaaataca aataccagag tacatactag attatataaa gtctgatata
     3481 attttaaata taatctatat gatttttctt ttaataatat atatttcggt tattagatgg
     3541 atttttagca ttcatgagat aactttaaat acagaaaatt ttaaaagagc aagaaaagaa
     3601 agtgttaatc ttataaaggg aaagattata agaacaatta tttattcatt aatattatct
     3661 ttagtagtag ctattattgg atatgcgatt tatcatagtg gaattatatt tataggcata
     3721 tggactaaat attgtactga tatggaattt ttgaagaatg tatttataaa tagatgtatt
     3781 gcatttgaag aatattttag attgatttta tcaatattca tttttatatt ggacattgga
     3841 tttatctcag caacttatta tcaatataaa ggagtaaata tttctaaaaa taatattaaa
     3901 aaagagaaaa aatctatatc aaaaattgtt tttaatttag tattaatatt attgatttct
     3961 tatatagagt cagttgcatt ttcttcacat ataaatggta tgtttaatac gtcattttca
     4021 ttttatacaa cagcaattgc tcatagagca ggagctgaaa tggctccaga gaatactttg
     4081 gcagcaatag aagaagcttc acatgctaaa gctgattatg cagaaataga tgttcaagaa
     4141 actaaagatg gagaacttat tgtacttcat gattcaaact ttaagagaac tacaggagta
     4201 gataaaaatg tttgggatgt aaattataat gaagtaaaga catatgatgc gggaagcttt
     4261 tattattatg gaaaaggtta ttatgatgaa aagtttgttg gggaaaagat accaacactc
     4321 aaagaaatga taaaatattc taaaggtaag gtgaaattat taatagaaat aaaacttaat
     4381 ggacatgaaa agggagacgt agtaaaaaaa gttgttcagt taattaaaga aaataatttt
     4441 gaaaatcaat gtatagttgc atctatggat aaaactatat tacaaaaagt aaaaaaactg
     4501 gatgagaatt tattgacatg ctattttatg gcaattgcat atggtgattt ttcaaagtta
     4561 aattatgttg atatatatgg aattgaatca acttttgtta ataaaacagt agtagaaaag
     4621 attcataaaa tgaacaaaaa aatcttcgtt tggactgtta atagtgacag tttaattgag
     4681 aaaatgcttg acctaaatgt agatagtatt attacagata atccatacct tatagagagt
     4741 gcaatttatt ggaagaaaaa tggatttatt agtcaagtgt cagattattt attttaaata
     4801 ttctataatt gtattatcca gtaaaagaga tattattaaa atcaataata tctcttttta
     4861 acataaataa ggttgttaaa ttaaggttat tcttataaaa attgtgctta tcagctataa
     4921 acaaggaata aaagtgaaaa aattgctctg agattattac agttaaaaga gttttaagat
     4981 gacagagaga tagattgtca actgtatatt taataaatct tgttatttat taaattttat
     5041 ataagtaaaa tatataaatt aataaatctt atgctagaat atatatgaaa aacaatataa
     5101 atttggagga ttaaaaacat gacagagcga gaaaaaatgt tggcaggaga actttacgat
     5161 tgtggtgata ttgagttgtt aactcaatgg cataaagcaa aaggcctagt aagagattat
     5221 aaccaaacag attcctcgga tttggaaaaa aaagaaaaaa tattaaatga gttacttggt
     5281 ggtaagggta aaaacttatg gattactgca ccgtattatg tagattatgg aaataatatt
     5341 tattttggaa ataattgtga agtcaatatg aattgtacgt ttttagatga taataaaatt
     5401 atcattgggg ataatgcact aatcgcacca aacgtacaaa tatatactgc atttcatcca
     5461 actaatgctg gtgatagatt tggtaatgcc aaagaagatg gttcatttga attttgtaaa
     5521 actcaaacag ctccagttac tattggagat aatgtttgga ttggcggggg tgctattatc
     5581 atgccaggtg ttactattgg agataatgta gttataggtg caggaagtat tgttacaaaa
     5641 gatattccaa gtaatatgat tgcatacgga aatccatgcc gtattattcg agaaaataaa
     5701 taaagttaaa ttttcttgca aaatctcaaa aaacaccttt cttactttat acatagggta
     5761 ttttctaata ctttcacatc catattcatt tgttaaattt ttgtggaatg tagtttttat
     5821 cacttctatc ttacatttcc accatttttt tataaaagtc attcctttat agatttattt
     5881 ataatagtat atattctatt tgatgtttga ataagcactt atttttaagt aaaatactaa
     5941 cttttaggtt agtattttgc tatagtatga tgattaagta agaata
//
LOCUS       DAMJZE010000211         5956 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJZE010000211 DAMJZE010000000
VERSION     DAMJZE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.215"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            135..947
                     /gene="modA"
                     /locus_tag="PC541_003449"
     CDS             135..947
                     /gene="modA"
                     /locus_tag="PC541_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2840870.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            957..1748
                     /locus_tag="PC541_003450"
     CDS             957..1748
                     /locus_tag="PC541_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840871.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1750..2445
                     /locus_tag="PC541_003451"
     CDS             1750..2445
                     /locus_tag="PC541_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2840872.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2432..3163
                     /locus_tag="PC541_003452"
     CDS             2432..3163
                     /locus_tag="PC541_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840873.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3477..4277
                     /locus_tag="PC541_003453"
     CDS             3477..4277
                     /locus_tag="PC541_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2840874.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4817..5533
                     /locus_tag="PC541_003454"
     CDS             4817..5533
                     /locus_tag="PC541_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2840875.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2316 a          720 c          842 g         2078 t
ORIGIN      
        1 aatagatttt ttcttaacta tacataacaa aacataacaa aacattataa aacataacaa
       61 aaacttagac atcgttttct tagtatgata tactaaaaaa gaatttacat taatttacta
      121 agggggaata tcttatgttt aaaaaacttt cagtactagc tttagctatg attatttcat
      181 taagtattac tgcatgtaat tctaaagaaa acaaagctaa tgatgaatct aatactaaag
      241 tgacaacagc tgatgattca aaagaaaaag agactctttt tgtctattca ggggcaggtt
      301 taaagaaacc aatggatgaa atagcaaaaa aatttgagaa agaaaataat gtcaaagttg
      361 aatatagcta tgcaggttct gcacagctaa tagctcaaat tgagacaagt cacaaagggg
      421 acgtatttat tgttggttct gaaccaattt attcaaaagc ctttgaaaaa gaacttgtag
      481 gtgaacataa aacggttgca catcatacac ctgcaatagt tgtacctaaa ggaaatccag
      541 ctaatattca aaaattggaa gacttaaata aagatggagt taaacttatt ttgggagaca
      601 aagagtctaa tgctataggt aagactacac aaaaaatact tgaaaaaaat aatttaaaat
      661 ctataaatga taatgttgtc tctacagctg caactgtaaa tgaaatgatt gtacagttaa
      721 catcaagtaa agctgatgct acaatagcca caaaagattc ggtctttggc aatgaagatg
      781 tagaagttat agaaattgcc caagataaaa atatagacca acttatatca gcaggaatcg
      841 taaactatag tgataaaaaa gatttagcta ataaatttat aaattatata gagtcagacg
      901 aagctaaaca gatatttaaa aaatatggtt ttgaacctgt taagtagtga ttaaacttga
      961 aaaataaatt ttttactact ctacttactg gattttttgc tatagtcttt ttgtttattg
     1021 tatcaccact tcttatgtta atcattaaag ggataagtta tgttcctata tgcttaaaat
     1081 cagtggaagt acagtatgca ataatactta gtattaaaac atctttaata tctactatag
     1141 tatgcctttt attagctatt ccagtagcat attttttaca tattactaaa ttaccattta
     1201 aaaaactgat tatacagata ataaatctcc ctatgtctct tccacactta gtatcaggaa
     1261 ttgctctttt acttttgttt ggaagaatgg ggattggaga ttccatttat aagattttta
     1321 agcttgattt tatatttaca aagcaaggta tagttttagc acaagtattt gtaaatcttc
     1381 cacttactat taaaatactt tacacttcac taaatgaaag taatgaaaag atgatatttg
     1441 ttgcacgtac ccttggatgt aatagttggg aagcatttag atttataatt cttccaaatc
     1501 taaaaactgg aatcatatct gcaactgtaa tgacttggtc aagagcactt ggtgaatttg
     1561 gtgcagtagc tatgatagca ggttctacac gtatgaaaac tgaaattatt cctacatcta
     1621 tatatttaaa tatgtctact ggtgatatag acattgcaat cggaatagct gtaattttga
     1681 tatttatctc acttacatgc ttgatgcttt ttgagatatt ttttaataga gaggttgata
     1741 agaattaata tgttaaaaat aaaaaattta tctcttgatt tacctaattt taaattacaa
     1801 gaaaatagtt taaatattaa taatcatgaa tactttgttt tattaggagc tagtgggtca
     1861 ggtaaaactc tatttctaga aacattagca ggaagatatc catctgctaa aggaaatgta
     1921 tcatacaaag aaaatttaat atcaaactta cctccagaaa atagaaatat aggatttgta
     1981 tatcaaaatt ttgaattatt tccaaatatg aatgttgaag ataatataag gtttccactt
     2041 aaattgagaa aaatacctat agatgaacag attcttaagg ctaaccactt atctaaatta
     2101 atgcagatag aagatataaa aaataggtat cctaaaaatt taagtggagg tgaaaagcaa
     2161 cgagttgctt ttgcacgtgc cttaatagct gaacctgata tattactttt agatgaacct
     2221 atgagttcat tggattatat tacaaaaaaa catataagca aaattttgaa ggatgtctac
     2281 ataaaataca aaccaacagt gattcatgtt actcatgata tttctgaagc aatgtttttt
     2341 gctcataaga taggaataat gaacaatgga aaaataaatc atatttttga tttaaatgaa
     2401 gaaataaaaa ataaaggaga atcttttttc tatgaatata tttgatattt tgagagatta
     2461 tctattagta caagttgata aatataattt aaatatggat atgataagta ttgtgtccaa
     2521 atctctatct tcaaaagaag caattggaaa tactaagcgt aaagatttcc caattatagt
     2581 tggtaaagaa ataatgcttg aagctgactt taaaggagct aaaggacaag catttacttc
     2641 tactccatct acatttgaag gttctttaaa agacatatta tcattagatt tacatgacaa
     2701 tcctcatgat agaagtcttt ttatagcatc attaaatgca gttatgaaat acttaggcaa
     2761 gacggataga accattcatt gtaaaaataa cgagccagag atatgtgcaa aaaaattccc
     2821 tgagtttata aaaatggaat ttggtaatcc aaaagtagca attataggat atcaaccagc
     2881 cataattgat aatattaagg actttttcga aacaagagta ttagatttaa atcctgaatt
     2941 tgtggatact atccaatata atgttaagat tgaagatggt atacgagatt atgaagatgt
     3001 aatatcttgg gctgatttag tgatttgcac aggaagtaca ttatgtaata actcaattat
     3061 aaattttcta tcacttaaca aaccagttta ctattatgga actacgatag ctggtgcatc
     3121 aaatatttta ggtttaaaaa gactctgttt ttgtagtaaa taagcaatca tatcatcaag
     3181 gagtacacaa atatatatgt gtactcttta aatttaaaaa gttattactt ttattctaag
     3241 tactaaagta aaattctatt aaaatgaatc ccgaataaat gtacaatccc tttaaatctc
     3301 aattcctatc aatcattaat attttgatta gtcttataca taaatgtttt ctttatttta
     3361 aactaataaa aaaattttat tccattaatc tactctatat ttttcagcat ttaagaaata
     3421 atttttagta ctttgtttga taatataact aagacaactt ttcaggagat gattaaatga
     3481 catttaagaa taaaaaattt actataaata aatataaaat tctatctatt attctagttt
     3541 tatgtttgat tttaaccgct tttagtagct atgtgaatta tagtcataaa aaatttaaag
     3601 attatgttgt cttctatgtt cagcatcaag atgatgaagt gttgtgggct gggagtgcta
     3661 ttgttaatgc tataaaagaa agaggaaaaa atcatgtatt tgtggtttta gtatctactg
     3721 gatgtggtat atccgtcttt gataaatata aaaaatatga aaacctaaca aatatacaaa
     3781 aatcagaata tagaaataga gaatttttag cttctcttaa tagccttggt gtaataaggg
     3841 aaaatataat attattacca gaaacaaata caaatggtaa tactgacttc aattacatgg
     3901 aaaaaattgc tcttacattt gaagaaaaat ttaaaaatgt aactcatata gcacatactt
     3961 ataagttaga tgaccattta caacatttaa aaaatggttc tgttatccaa aatttatata
     4021 atgctggtaa aataaaagat gctaaatatt ttgtaaaacc taaatttgct gataaaataa
     4081 cttttaataa taagatagtt tataaagctc attctactga agactatgaa aaggttaaaa
     4141 atgcctgtca acaatacaaa attgttgatg aattagaata tagagaaggt attggctata
     4201 agtctgacca taaatcattt gataagttat taaataataa agatgttcct gctatacttc
     4261 atacacctaa tttataagaa atatttattc ttttaattta agttataaaa aggaaattat
     4321 ttgagatttt gtgatatagc actttttgtg ctatactact ttttctcata caactattac
     4381 ccatatctaa tttcatcatc tcttaaaata tataaagata tcatcattaa aacctataag
     4441 ccttattaaa tgctttatac atatctacat taagctacct atttatatta aactacatta
     4501 aaataaatat ttgttataaa ttcttaataa ttatgaaact catttgttat ttatatttgt
     4561 taatatatgt ttaaacctaa aaaattaata aaccaaaata acataaggag tgtgatagat
     4621 taccctagat attttcataa acttttacct tcagattaat atgggcgtct atacttttaa
     4681 atttactata tgcattgaca atatattaaa agtactagtt tttatttcat aatgaacggg
     4741 cttactgttt tctaatacct ctttttggta taaaataaaa attggttaaa aataaaattt
     4801 caagggggtt aataagttga atcaaaatat attggttgta gatgatgata gagagatagt
     4861 tgatgctata gagttctatt tgagacctga taactttaat gttttaaagg catatgatgg
     4921 acttgaagca ttggacatac taatcgaaaa tgatattaag ttaataataa tggatgtaat
     4981 gatgcccaat ttagatgggc ttaaaactac acttaaaata agagaaaaaa ggaatatacc
     5041 aataattctc ttatctgcaa aatcagaaga tatggacaaa attataggtc ttaatatggg
     5101 tgctgatgac tatataacta aaccatttaa tccactagaa cttactgctc gagtaaaatc
     5161 tcaacttaga aggtatataa atttaggtga ttttagtaat acaaataact caaatgatac
     5221 taattatatt ctaaaaagtg gtggcctaga gctaaataca gatacaaaaa tagtttcttt
     5281 agatggtgaa gaaattaaaa tgacaccact tgaatataaa atacttacac ttcttatgtc
     5341 acgaaaaggt aaaattttct cttctcatga gatttatgaa aatgtctgga atgaggatgc
     5401 ttatagttgt gagagaacta ttgctgttca tattagaaga attagagaaa agattgaaat
     5461 taatccaaaa gaaccaaaat atttaaaggt ggtgtggggc attggttaca aaattgaaaa
     5521 acttaattat taatgattct aaagcaaaat ttattttatt attattactg tttctattag
     5581 tttctatatt atttgaagca gtttatgatt tgaataataa cttttcttat aatattaatt
     5641 attattataa agagtttgct ctcccttatt tttatttttt cggtactcct gtacatttgt
     5701 caaaagtaga attagagttt gtctgcatag gtattatatt atcaagtttt ttattgacta
     5761 tactatgttt tacattgtat aaaaaaaata aacagactaa agtcaaattc ttagagaatg
     5821 tcataagcct tttttctaag gtaaaaatag aacttacact tatattaact ttgatactat
     5881 taaccttaag gtttcatgca tttacaagta ataaaatgtt ttacaacact aatttaataa
     5941 tctacaattt tataat
//
LOCUS       DAMJZE010000212         5901 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJZE010000212 DAMJZE010000000
VERSION     DAMJZE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.216"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            105..671
                     /locus_tag="PC541_003455"
     CDS             105..671
                     /locus_tag="PC541_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2840876.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            711..1292
                     /locus_tag="PC541_003456"
     CDS             711..1292
                     /locus_tag="PC541_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2840877.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1349..2266)
                     /locus_tag="PC541_003457"
     CDS             complement(1349..2266)
                     /locus_tag="PC541_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2840878.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2396..4027
                     /locus_tag="PC541_003458"
     CDS             2396..4027
                     /locus_tag="PC541_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2840879.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4030..4977
                     /locus_tag="PC541_003459"
     CDS             4030..4977
                     /locus_tag="PC541_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2840880.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5262..5525)
                     /locus_tag="PC541_003460"
     CDS             complement(5262..5525)
                     /locus_tag="PC541_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2840881.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5624..5869)
                     /locus_tag="PC541_003461"
     CDS             complement(5624..5869)
                     /locus_tag="PC541_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840882.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2127 a          807 c          976 g         1991 t
ORIGIN      
        1 ataaatatag ccaacttagc ctaaatacgt atataatata gctaatatgg ttcttatact
       61 tgataccatc aataaaacta aaaatataaa aaggagaact taaaatgagt gttagtttac
      121 aaaaaggaca aagagttagc ttagtaaaag ataataatcc tgtaaaaaat ctagtagtag
      181 gcttaggatg ggatatgaat aaattgggta aaaaaaatta tgacctagat gcatttgcaa
      241 tagcattgac taaccaagat aaaatgaatg ttagaggtga cttggtttat tttggaaatc
      301 ttaaacaccc ttctaaagct attatacaca ctggagataa cttaactgga aaaggtgaag
      361 gtgatgatga acaaataatt gttaatttag aagacatacc tgaatatgta cataaaatag
      421 tttttggagt ttctatatac aaagctaaaa aaagagacca ggattttggt caaataaaca
      481 actcttttat aagattaatt gattcaaatt ccaagcaaga attatttaaa tataatcttc
      541 aagaagactt ttctggtaaa gtaacagttt tggcaggaga aatttatcgt agaaatgaag
      601 aatggaaatt taatgcttta ggaattggtc aaaatgaaga gcttcgtgaa ttaattaata
      661 catataagta ataattacaa taaaaaattt ataatgagga gttggtttaa atgggtataa
      721 ctttagcaaa aggacaaaaa gtaagtttaa caaaaagtaa ccctggtcta aaaaaagtaa
      781 tagtaggact aggatgggat attaataaat atgatggtgg ctttgatttc gatttagatg
      841 cctctgcatt tttaactgga actgatggaa aagtaacgaa tgatggagac tttatattct
      901 ataataattt aaaacacgct tctggagctg ttgaatatat gggtgataat agaacaggtg
      961 ttggtgatgg tgatgatgaa caaataaatg tagacttatc taaaatacct caaaatattg
     1021 ctaaaatatc atttagtgtt actataaatg aagcaattac aagaagacaa aatttcggac
     1081 aagttgaaaa ttcatatata agaatatata atgaagaaac taatgaggaa cttattaaat
     1141 atgaattggg tgaagatttt agcatagaaa cagcaatagt tgtggcagaa ctatatagac
     1201 ataatggaga gtggaaattt aatgctttag gttctggatt tgaggatgga cttgcaggat
     1261 tgtgtaaaaa ttttggcgtt aatataggct aatatatcta aataaaaata agagtatatt
     1321 gatatactct tatttttatt tagatatatt ataaactgtt tacacttcta ctcttctttc
     1381 tcgcttgttt tttcccatat tctactgaaa tatcaaaaaa tgctgaaata ttttctgtaa
     1441 aaaacttgct tctatgatat gcaatatcaa atgtcctatg taaattaata ccttcaatat
     1501 cacaagccca caaaagatgc ttttttaaat aatcctcaat aagtaattcg gatataactg
     1561 caatgccaaa attatctaca actgctctta atatagcttc agagttataa catgaccatc
     1621 taatattcat tgggattttt aattctttta attgcttttc taattgtgct cttgtaccac
     1681 ttcccaattc acgcataatt aatggttgat ttgaaagttc tgaaactttt atagagtctc
     1741 ttttataaaa ctcatgcttg tgtgaacata taagtacaag attatcatta ataatgcttt
     1801 ttgtaacaat atctgggtgc ttaatttttc cttctacaat cgcaatatct aattcactat
     1861 taagtaattt ttttgcaatc agtcttgtat cttctacaaa tacttcaggt tctataagag
     1921 gatttacctt gtataattct aacattattg gacttattac acatgtacca acagttactg
     1981 ttgccccaac tcttatacgg atattttttg cacaatgctt catacaatca ttcatttctt
     2041 catttacagc taaaagatgc ctcccatatc caagaagttt tttccctgcc tcagttaaat
     2101 ataatttttt tgaaattcta tcaaatagct taacaccata ctctttttct atttctgaaa
     2161 tagcttggct aactgatgac tgggtaatat acatatttct tgctacttca ctcattttac
     2221 cacattcagc aaccttaaca aagatttcaa gactacgaat tgtcatatta tcacaacctt
     2281 agcaaacatt agtaaatgct tatgatttta ttaatatttt agtattttac aaatatattg
     2341 tcaagaagta taattttatt atagagttat aaagtagatt ggagtgtgat atattgtgaa
     2401 agtattaatt ataggtggag ttgctgctgg aacaaaaact gctgcaaaaa taaaacgtga
     2461 aatgggtgat aattgctctg ttacaatact aaataaaggt gatgatattt cttatgcagg
     2521 ttgtggtctt ccatattacg ttggtaagat aattgaagat aaatcatctt taatcgtaaa
     2581 tacacctgaa agtttttcta aactaacagg agcagaagtt cgttgtggag ttgaagttac
     2641 atctattgat agaaatgcaa aacaagtaac tttgaaaacg ctttcaacag gagaaaaaga
     2701 aaatctaagc tatgacaaac ttgtaattgc tacaggggca gacccaataa aaccaccaat
     2761 acctggaata gatttacctg gagtattctt tatgcgtact ccaaatgatg ctataaaact
     2821 aagagattct attgaaaacg gtattaagag agctgttgtt attggtggag gtttcattgg
     2881 tcttgaaatt gctgaaaatc tttctgcaat gggtatacgt gtaagtgttg ttgatatggc
     2941 tgaacatgtt atgcctggat ttgatactga ttttgctgaa tacgttgaaa atcatatggc
     3001 tgataaaggc ataatgattt ttacaggtga ccaggttgta ggtattgaag gtgaaaataa
     3061 agttgaaaaa cttcgtacta aaaatagagc tattaaaaca gatttagtgg ttatgtcagt
     3121 tggaatccgt cctaacacag gttttctaaa agacacaggt ttagaatttg cccctaataa
     3181 tacaattatt gcagatgaat atatgcttac aaatgacaaa gatatatatg tagttggaga
     3241 ctgtgctttt gtaaaaaatt ctttaacaaa taaacctaca tggtcaccta tggggtcatc
     3301 tgcaaatcat gagggtagaa tttgcgcaca aaatatatct ggaaaatcta aaacttacaa
     3361 tggtgttctt ggaacaacag tagtaaaatt acctgagctc aatgcaggaa aaacaggtct
     3421 aagtaaagaa atagctgaaa aagaaggtta taatgtttgc tctgtaacca tagctactga
     3481 tgataaagct cattattatc caggtgcatc taattttatt ataagaatgg ttgctgaaaa
     3541 aaatactaga aaattattag gtgttcaagt tatgggacct ggagcggttg ataaaatagt
     3601 agatatcgct gctactgcaa taactttagg cgctgacctt tattcattag aaagtatgga
     3661 ccttgcatat gcaccacctt tttcaacagc tattcaccct tttgtagtgg cagtaaatgt
     3721 tttacaaaat aaacttaatg gagaacttga tagtgtttta cttgacgaaa ttgaggattt
     3781 tgacagttgg actaaacttg atgtttcaaa agctccttct attcctagtt tacgttatat
     3841 tcctgttggt gaaattaatg gagaaattga aggtcttgct aaggatgaaa aaattcttct
     3901 tatatgtgct aaaggtaaaa atagctatat ggcgcaaaat cgcttaagac gctttggcta
     3961 tacaaatacg aaagttcttg aaggtggaat actattttat ccaaatctta aggtaatgga
     4021 ggagtaaata tgagtaatgt tacagcagaa caaaaaaagg aagtaaaagg tagaggtttt
     4081 ctaccaagca gggatggaga acattttgca gcacgtataa taactgtaaa tggtgtaata
     4141 aatgcatcac aagcaaaaaa aattgcagaa gctgctgaaa aattcggaaa cggtcagtta
     4201 gcatttacta ctagactaac agtagaatta cctggtgtaa aatttgaaga tattgaagct
     4261 ctaagtgaat ttattgctag cgaaaatcta ataacaggtg gaactggatc aagggttcgt
     4321 cctgttgtgg cttgtaaagg tactgtttgt gttcatggtc tagctaatac acaggcactt
     4381 gctgctgaaa tacatgaaga attttacaaa aattggtatg atgtaaaact acctcataag
     4441 ttcaaaattg gtgttggtgg atgccctaat aactgtatta aacctgacct aaacgatctt
     4501 ggaattgttg gtcaacgtgt acctgactat gattcagagc tttgtgttgg ttgtaaaaaa
     4561 tgtgctgttg tagaggtctg tcctgttaaa gcagctaaat taacagataa aggtaaactt
     4621 gaaatagata gtaatctatg taataactgt ggtaagtgta tagaaagttg taattttgat
     4681 agtattgaag aaaaagaatc tggttataaa gtctatatag gagggaaatg gggaaaatca
     4741 gttcgtcctg gaacacagat tgatagatta ttttcaaaag aggaacttat gactttaatt
     4801 gaaaaagcaa ttcttttatt tagagaacag ggtaacacag gtgaacgttt tggaataact
     4861 atcgatagaa tcggtgctga taaatttata gaaatgcttt tatcagatga agttttggaa
     4921 agaaaagaag agatactaga ggctctttta caccttacag gtggagctgt atgctaacaa
     4981 taaatatttt aattgtacag ataacctatg gaaaaaattt tagttaagaa taattaaaat
     5041 tctatatatg atatatgata aatgaggcta tctcaaaaca cagatacgcc tctttttcat
     5101 tgttgaaatg ataaagtata tttgagttca tataaaaaag agtgtctcat gaattataaa
     5161 attcattttg aggcacccac attattttat aataattgct tattaatttt attttaacta
     5221 tataaaatgt acaaaaatta agaatatcca atattctaaa attaattttg aattgtattg
     5281 attgaaaagt ttactattcc actatttcca tcttgattta ctactagata ccatgttcca
     5341 gtattaggaa ctctcattct acatggattt ttctttgcaa atccaccata gtaatcataa
     5401 tttctttgat ttttaaattt cacataattt atatggtcta acaatattac attggcttgt
     5461 ttctctaaat ttacctctac aatatcccct ttttttaagt tcatcttact ataactatat
     5521 tccattgaat tactcctcca gtatcgaata atctttgtta tttactcatc tcaaatatgt
     5581 aaaaattcat ttaaaatatt acaaatacta tataattaga ctattatcca gttttaacct
     5641 tatttactac aatccaaagg ttattaaaaa tattattggc aacaacttct ccatcccata
     5701 caactctttc ttctttattt tctaataaag catagtaccc agagtcagaa ctttcatctt
     5761 tatagactgt aacataaaac tcttcatcac tatattcagg tatattttta aagtcttcag
     5821 atattttaca tgttaatata taaccttcta attcttcatc tataaacaaa gcaattcctc
     5881 caatctctat atttttacta a
//
LOCUS       DAMJZE010000213         5860 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJZE010000213 DAMJZE010000000
VERSION     DAMJZE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.217"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            106..846
                     /locus_tag="PC541_003462"
     CDS             106..846
                     /locus_tag="PC541_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2840883.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            858..2636
                     /locus_tag="PC541_003463"
     CDS             858..2636
                     /locus_tag="PC541_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2840884.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            2803..3720
                     /gene="pfkB"
                     /locus_tag="PC541_003464"
     CDS             2803..3720
                     /gene="pfkB"
                     /locus_tag="PC541_003464"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2840885.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            3757..5676
                     /locus_tag="PC541_003465"
     CDS             3757..5676
                     /locus_tag="PC541_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2840886.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         2413 a          539 c         1082 g         1826 t
ORIGIN      
        1 aaccaaatac taaaaaatat tttaaataaa gaacttaaaa ttgtgataaa attaatttat
       61 agatattaaa aaaagtataa tatatggttt ttaagaggtg ctaagatgaa aaattttaga
      121 gatttaggtg ggaacaaaac tgaggatggc agaactgtaa agaagggatt attttatagg
      181 tctgcaaaat tatcaaatct aagtgaaaat gatataaaga ttttaaaaga gttaaatatt
      241 aaatatatat ttgattatag aagtgatgaa gaagctcgta aacatccatc aacaataata
      301 tcaaatataa aaaatataag aataccagca atgagagagc tagaagaatc aggaggaagt
      361 tttggttcta ttgaagatat gattgatgga ttgtttgaaa aagatggtgc atttaatatg
      421 ttaaataata gttactataa tttgcctata aataatccgt catataaaaa attagtagaa
      481 cttataagag actattctaa tttaccaata ttaaatcatt gtacagctgg taaggataga
      541 actggtgtgg ggagtgctat aattcttatg attttagggg tatcgagaga gaatataatg
      601 aaagattatc taaaaagtaa tgattttgca gataaagaga tagaaagatt tatagaatat
      661 aagcctaaat ttaaggacat tccaaaagaa aatttgaaat atatatttgg tgtaaatgaa
      721 gagtatatga aaactgcatt tagaagaatt gatgaagaat acattagtgt agaagcatat
      781 ttatatggag aatttaattt aaataaagaa gaaataagaa aattaagaaa tcaatatcta
      841 gagtaaaagg tgagtttatg agtttaaata taacagcaag acagataaat ataataaaaa
      901 tattgttaaa ttcaaaagat acaattagtg gtattgcctt aagtcaagag ataggatgtt
      961 cttcaaaaac tatacaaaat gaaataaagg atattaataa acaacttaga aatggtaaaa
     1021 ttttgtcaat aagaggagtt ggttataaac tagaaggaag ttttgatgag gtaaatctaa
     1081 atagcaatct ctatgatgat gtagatagag tagaatatat aatcagaaaa atattaacct
     1141 taagtaatga agaaaaaaat actataaaac ttgaagattt agccgattct atgtatgtaa
     1201 gtgtttcaac agtaaaaaat gatttaaaag aagtaaaaag aatgttggaa aagtattcaa
     1261 tatcagttaa atctaagcat aaacaaggta tatgtgtttt ggaaagtgaa gataagatat
     1321 taaattgtat agttgactta agcaataaga gagataatca attgagttta aatgattttt
     1381 taaatgatga tataaaggaa agaaaactat taataaaaaa attattatta gacatattaa
     1441 atgaaaatgg attagtatta actgatatag aatttaagag tgtgttaaat aatatattag
     1501 ttatgttaag cagacataag tataatgaaa aagaatttat agagaattat atagaaaatt
     1561 ataggagcaa aagaagctca attttagaaa atgaacaaaa caaagaaatt ataataaatt
     1621 caataaaaac attttgtaaa aatttaaaat tagctacatc aatagatata agtaatgata
     1681 aggtatttga agagtttttg tataagcata ttagtagttt atgtaaaaaa ataaagttag
     1741 gtataaatca atcacctata atggctcaag atataaaaat aaaatatcca tttgcttttg
     1801 aactagctaa tatagctaaa aagacaatag aaaaagattt gaagatagaa attaatgaag
     1861 atgaagttgc aaacatagct ctacatatag gaggagcagt tgaaagagca tcttataata
     1921 ataaagataa ggttttcaaa actataatag tttgtacttc aggcataggc acatcaatgt
     1981 taataaaagc taaattagag aatatattta aggaaaaatt agaaatacta aagattattc
     2041 cttcatactt agttgaatat ataaaagtta tagatgttga ttttgtaata tctactgttc
     2101 ctattgaagt aggagatgtg ccagtaataa atatatctcc aattttaagt gagaaagaaa
     2161 ttagattaat tgaaaaatat atagaaacag gaaatatata cctagattta gatataaaaa
     2221 gcctgtttga tagtgaattg ttttttactg atttggtgtt taaaaggaaa gaagaagtta
     2281 tagattttat ggcaagtaaa cttgtacaaa agggctatat agatgaggat atgaaaaaat
     2341 cttactttga gagagaaaaa atagcaacaa cagaaatagg gaatatggta actataccac
     2401 atggagctat tggaaaaatt tttgataata aaatagcaat tggtatatta aagaagccta
     2461 tttcttggga aatatcagat gttcgtttaa taataatgtt agcccttgat aaagataaaa
     2521 tattagatta tgaagtaata ttttcaaaaa tatataaaag agtagattct atagcaaaag
     2581 ttataagtat atgtgaaaat agtagttttg aaaaatttgt aaatttattc aattagatgg
     2641 gtagtattgt atatacacct gtaaatttgg ataatataaa actaaataag ataaaaacga
     2701 taaatttctc ttttaattaa gagaaatttt ttgactttca aggatattaa tataaagtta
     2761 agatataaac ataaagcaaa taaataaaaa agaggtggca atatgattta tacagtgaca
     2821 ttaaatccat caatagatta tatagttaag ctagatgagc taaagacagg aagtacaaat
     2881 cgtgtaaaag aagaatacgt ttatccagga ggaaaaggta taaatgtatc tcgcatattt
     2941 aaggaacttg gaaatgataa tacatcttta ggttttataa gtggttttac tggagaatat
     3001 ataataagaa cattggaaga aaaagaattg aagacagatt ttataaagat aaaaagtggt
     3061 ttttcaagaa taaatgtaaa aataaaggcg ttaaaggaaa ctgagattaa tggacaaggt
     3121 ccaaatatag atgatgaaga cattgatact ttatataaaa aacttgataa gctaaatcaa
     3181 gatgatattt taatattggc aggaagtata ccatctactt tagatgaaaa actatacgaa
     3241 aatataatgg ctagattaga aaaaaagaat ataaaagttg tagtagatgc aactaaaaat
     3301 cttcttctta atgttttaaa atataaacct tttctaatta agcctaataa cgacgaatta
     3361 gaagaactat ttggtgttaa attaaattca attgaagaca tggtaaaata tgctagaagg
     3421 ttaaaggaaa tgggagctat aaatgtcttg gtatcaatgg gaaaagatgg agcattgttg
     3481 attacagagg aagatgaagt gcttataagt gatgttccaa aaggaaaagt aaaaaattct
     3541 gtaggagcag gtgattcaat ggtagcagga tttatatctg ggtatttgaa tactgggaaa
     3601 tatgattatg ctcttaaatt gggagcagct tcaggtagtg caactgcttt ttcatatgac
     3661 ttagccaaaa gagaatacat tgataaattg gtaaatgaga tatctgtaaa gcagttttaa
     3721 aatattaaaa aatatataga aaggtaatta atatttatga aaattataga tttattagat
     3781 gaaaagtcaa ttaagttaaa tttaaattca aaaaccaaat cagaagcaat agaagaatta
     3841 gtagatttgg tcgcaaatag tggaaattta aatgataaag aaaactacaa aaaagcaatt
     3901 ttggctcgtg aagaaatgag tacaacagga attggagaag gtgtagctat acctcatgct
     3961 aaaaattctt cagttactaa agcttgtata gcagcagcag tgtcaaaaga aggtatagat
     4021 tatgaatcgt ttgacggaag tttatcaaat ctatttttta tgatagctgc accagacgga
     4081 gctaacaata ctcatcttga agtattatca agattatcta caatacttat ggatgaagac
     4141 tttagaaata aacttataaa ttcaagctct gaaaaagaat ttaaggaaat aatagataaa
     4201 aaggaaagag aaaaattcag tgaagaatat caagatgaga aagttactga aaaaaatata
     4261 atagatacta aagagaatga tgctaataag tatcctaagg ttttagcagt aacagcatgt
     4321 ccaactggaa tagcacatac atttatggca gctgaaagtt taaataaaat ggcagaaaac
     4381 aaaggtgtat caataaaagt agaaactaat ggttcagcag gtgttaaaaa taagcttaca
     4441 aaagatgaaa tagaaaatgc aacatgtata atagtggcgg cagataaaaa tgttgagatg
     4501 gctagattta atggtaaaaa agtcataaaa actaaggtgg ctgatggtat acacaaagcg
     4561 gaagaactta tagacaaggc tgtaaatgga gatgctccaa tttaccatgg aggagatgga
     4621 agtcacaacg aatcaaatga agaaagtgaa agtggattta gaaaagtata caaacactta
     4681 atgaatggtg tatctaatat gttaccattt gttattggtg gagggatact tatagcgata
     4741 gcctttttac ttgatgatta tactattaac ccttcaaact ttggttcaaa tacacctata
     4801 gcagcatttt ttaaaggaat aggagataaa gcttttggat ttatgttacc tgtacttgct
     4861 ggatatatag catactctat atctgataga ccagcgtttg ttgtaggatt tgtaggaggt
     4921 gctctagcag gtgatggtgg ttcaggtttt ttaggtgcac tacttgcagg ttttatagca
     4981 ggttatttag tagaagggct taagaaaata tttagtgtac taccagcttc attggaagga
     5041 ataaaaccag tattgttata ccctttactt ggaacacttt tgatgggaat tataatgaca
     5101 tttttagtaa tacctccagt tacagcaatt aataatgcaa tggttggatt tttaaatggt
     5161 ttaggtggaa cttcaaaaat agttttagga ttagtattag gtggaatgat ggctgtggat
     5221 atgggtggac ctgtaaataa agcagcatat gtatttggag ttgcatcact tgaatcaggg
     5281 cagtttgaaa taatggcagc agtaatggct ggtggtatgg ttccgccact tgctatagct
     5341 cttgcaacta cattttttaa gaatagattt actaaagaag aaagagattc tggtaaagtt
     5401 aattatgtaa tgggattatc atttgttact gaaggagcta taccttttgc agctggagac
     5461 ccacttcatg ttataccagc ttgtgttgga ggttcagcag tggcaggagc acttagtatg
     5521 ttgttcaacg cagctcttcg tgcacctcat ggaggagtat ttgttatacc agtggttacc
     5581 catccatttg catatatatt agctatagct gtaggtgctt tggttggaat gatgctttta
     5641 gcattactta aaaaaccatt aaatcaagaa gtatagcaat taaaggatat tcaagaactt
     5701 aaaatatgat gttttataaa aattaatata ttgctgaatt attttaaaat aaggatttta
     5761 tgaggctgtt atataacgat ttgtaattgt tatcttgaca gtcttttatt tttatataag
     5821 taaaataagt gtgttttgct ttatttgtat ttaaattgtt
//
LOCUS       DAMJZE010000214         5828 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJZE010000214 DAMJZE010000000
VERSION     DAMJZE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.218"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..439)
                     /locus_tag="PC541_003466"
     CDS             complement(<1..439)
                     /locus_tag="PC541_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2840887.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMII"
     gene            complement(587..1378)
                     /locus_tag="PC541_003467"
     CDS             complement(587..1378)
                     /locus_tag="PC541_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2840888.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1414..2070)
                     /locus_tag="PC541_003468"
     CDS             complement(1414..2070)
                     /locus_tag="PC541_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840889.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2063..3028)
                     /locus_tag="PC541_003469"
     CDS             complement(2063..3028)
                     /locus_tag="PC541_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2840890.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3134..3240)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3530..4474)
                     /locus_tag="PC541_003470"
     CDS             complement(3530..4474)
                     /locus_tag="PC541_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2840891.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            4585..5052
                     /locus_tag="PC541_003471"
     CDS             4585..5052
                     /locus_tag="PC541_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840892.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(5222..5821)
                     /locus_tag="PC541_003472"
     CDS             complement(5222..5821)
                     /locus_tag="PC541_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840893.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         1895 a          981 c          627 g         2325 t
ORIGIN      
        1 taataatcat attccagtta atatcactaa aaatatttct tataaagtaa tctaatatca
       61 caaaagcact ataaaaaatt agtattgggc ttattcttag tttttttact ttagaaatgg
      121 ttaagtatat aaaagtagac atattaatta tgcttaagtt aagcaataca atttggaatt
      181 tttgaatttt taaatttact actcctaaaa tacttacaaa aactaataca agacacccta
      241 ctttcaatat atttataaat tcatctgttt taaagctact tatataaact atagaatatc
      301 caaatatcca aagtaaaatt attctataca tatttataga aactgtatac aaatttatat
      361 ttaaaagtgc aataaccaaa aataacccat tagctagtat atagacctta ttcttactta
      421 aagttccttt agattccata ttccccacca cttcttcatt tttatatagt tttacctata
      481 aatatgttac cacaattctt ccaaaaaatt tgtcactttc tagttataaa aaaaaatgag
      541 taaatatttt taattttact cattttgtag attttgtata ttcagtttaa aaagctggaa
      601 ctatacttcc tttatattca tcttgtataa attttttaac ttctttacta ttcatagcct
      661 ctgataaagc cttaatctta tcactatctt tattattttc tctacaagct attatattta
      721 catatggaga atcacttgat tctattacta ttgcatcctt agtaggattt aagttagctg
      781 ttaaagcata attagaattt attactgctc cgtcaacatc ttttaatact gttggtaatt
      841 gctctgcatt catttctttt attactatat ttttaggatt tttagttata tcttttttag
      901 ttataagctc tccatctttt acttctatca atttattttt tgctaataat ttaagtgctc
      961 tagctccatt agttgcatca tttggcacag ctataacagc tccatctttt aattcatcta
     1021 atgacttaac tttttctgaa tagaatccca ttggctctat atgaacttta gatgtataag
     1081 taagtttata tcctttctcc ttcacagttt cctctaaaaa tggaatatgt tgaaagaagt
     1141 tagcatccaa agacccttca tctaaagctg tatttggaag tacataatca tcaaatattt
     1201 ttacttctaa atcatatcct ttttctttta acaaaggttt tgcaacttcc aatatctttg
     1261 catgtggatt tgatgaagct cctactagta tttttttatc ctctttgttt gaacaaccta
     1321 ctgctgatat tgctattgct gacaccaatg ctactgataa taatttcttt aatttcattt
     1381 ttactccccc tctttaaaat gtttataatt aatttatttt agtttcttat aagccagatt
     1441 tcctactccc tggattatct gaactaatat tataagtgct attacagtat ataccattac
     1501 tgctgtatca aatctttgat atccataaat aagtgctata ttacctaatc ctccagctcc
     1561 tactgcacct gccatagctg tataaccaag tatagatatt attgataatg taattccaga
     1621 tactattgaa ggcatagctt cctttatcat aactttaaat actatttgtc tttttgtagc
     1681 tccaaaagat tttgctgctt ctatcaatcc tttatcaact tcatttaatg agctttctat
     1741 aattctagcg ataaacggtg ctgccccaat agttattggc actattgcag cagtttcccc
     1801 tatacttgtt ccaacgatta atctagttat tggaatcatt gcaactataa gtatcataaa
     1861 tgggaaactt ctaaatatat ttactataaa tcctaatgca ctatttattg tgctatttgg
     1921 ttttaatcca tctggttttg taactactaa tatgattgct aatataaatc ctaatattgt
     1981 tgccactata gttggtacta taaccatata caaagtttgt aacaatgcat ttggaaatag
     2041 tgttgttaaa aagtcaatta aactattcat ttatcatttc ctcccatctt atgcccttac
     2101 tagttagata ttgttttact tgctctccac ttttatctga aatgtttata ataagtgaac
     2161 ctaaaatatc atctttaaat tgttctaatt tcccaaatat tatagacaca tctatattta
     2221 gttctcttgc catagtcgta ataatagctt cattagaaat atctttaggg aatagtatct
     2281 ttatatttgt tccttttggc aatattatac tttcttcacc tattaatttt ctaagtcctt
     2341 tagtatttct taagaatatt tcttctgtat ctcctacctc taatacttct ccattttcca
     2401 ttattgctac tcttccacaa atctgcttta taacttccat ttgatgagtt acaactatta
     2461 ttgttattcc caatttttta tttatatctt caagcaatga aagaattgac tttgtagtat
     2521 ttgggtctaa tgctgaagtc gcttcatcac aaagaagtac ttgtggattt agtgctaaag
     2581 ctctagcaat tgcaaccctt tgtttttgtc cgccacttag atttctgggt ttatcatttt
     2641 tcttttcaga tattccaact acttctaata actctaaaac tcttttttta atttctgcct
     2701 ttgagtatcc aaaacattct aatggcaatg ctacattatc aaatacagtt tttctttcaa
     2761 gcaatgaaaa gtgttgaaaa atcattccta actcttttct taaatctctc atttgctttt
     2821 cattcaatga ctttacttct ttatcagaaa caagtacact gccttcttgg tattcttcta
     2881 atccgtttat acatctcagt agtgttgatt tccctgcacc actatgacct attattccaa
     2941 aaatttctcc agattctatt tcaatactta catctttaag cacttgaatc tttccataat
     3001 acttattaac attttttatg ctaatcataa aatttcctcc catatattct gctcagtttt
     3061 tatgtttatc ctctcttatg tattacaaca aaataaaagc ccgagtataa actcaggctt
     3121 agaaagctat ttatactcat ctatcaacta tattagttgc aggatttagc accaatgtat
     3181 ataaaatata ctggttgccg ggtttcaaag ggccagtccc tccaccactc ttgataagac
     3241 gttatttaat ttaattctat tatcatttgt tgctatgtta tatatgatat attcattaat
     3301 agtatttgtc aataaattta tttaaatttt ttatttttta ttatcgttaa ttaactattt
     3361 ttaattagtt ttctagctcc taacacttta tattttaatg tctatataac tatagaacat
     3421 cattataaga aataaattaa ataacatcaa tctatctaaa tcatctgtaa agtaaattaa
     3481 gaaaattaac attttatcca aactctatcc tactattaaa taaaataatt taaatacaat
     3541 gatgattata tagctctagt actttagact ttatcaaatg tttaagttct ataggttcta
     3601 ttattgtagc atttttccca aaagttaaaa aatattcagc cacccagtta agtgtagatt
     3661 ttggtatata catatctatg tatccacctt tagacaagat tttcaaccct cttgccaaca
     3721 aaaattctgt ttcacaacgt tttactccct catctgtcaa attaatttta atatgataat
     3781 ctgtacctac ttccatattc ttaagataat catgtataga agataaaacc ttatatttct
     3841 tacttgataa gttttctata ctcaaaatct tcacaattct gtctacacga aattccttaa
     3901 tagattctga ttttatacag taagctggac aataccaaag tccattcatg gcatacaaac
     3961 caataggaac aatggtacgt acactatttt tttgctttga tgaatattca attttaattg
     4021 taaattcatt cattactata ttaaacaatt ctttcaataa tggtgttgaa cagtgcctat
     4081 ctggtgtcca aaaaacaaat ttacgcctta tattctcaat attatattga atatcatttg
     4141 gtatacaatt taaaaatttt tttaaaacag aaatagtttc ttgctcaaat ggcaaatcat
     4201 tataatattc tagtgactga tatgcaaaga atatagattt tacctcactt tctgaaaatg
     4261 caatcggtgg aagtatacgc tctttaagta catgatatcc accagccgct ccaacttttg
     4321 aatataatgg aaatccaata ctctccagca cctgtaaatc tcttagtata gtacgttttg
     4381 atacagaaaa ttcttctgct aattctcctg ctgtaaaact tctttttgca tttattgtta
     4441 tcatcagttc aattaaacgc tcagctcttg acatatttat actcctaatt taaatatagt
     4501 gacaataatt gtcactatta tatgataaga tattcttgca tctaaagcaa taataatagt
     4561 aaagaaagaa gggattgata ctttatggaa aatgatacta ttaaggctga tgatattctc
     4621 aattattgtc tatcaaactt agatgatgtt gtactaatgg atagttgggg ggaacgagca
     4681 atttactaca atcctaatgg tgttttaaag cgaggggtat atgttcttac cattaaggaa
     4741 aaagacagta ataatgataa aggttcgtta gttagtcgtc caaatgtata ccgtgtgaat
     4801 ataggattaa aaaaagaaac ttttattgaa atgtttggat atattccaaa gcgtccaggt
     4861 gtaggtcaaa tagttgatat ggattttgat tttacaaaat tggacacaat catgccacat
     4921 cctatctact catggatggg atggatatgt gtcctaagcc ctactgaaaa gacatttgag
     4981 aactttaaaa ctttaatagg agaatcttac aattttgcaa aacaaaaatt taaaaagaga
     5041 aaaaacaagt aaaaacgtgt gaaaataacc tctaaataaa ttataataaa aatattaagg
     5101 ttacaagata aaaattaata tcttaataac atgcaaaaaa gcgaagccaa aaaatgtatt
     5161 atttataagg cttcgctata tctcaaataa tttatttcaa atttcaaaac tatttattct
     5221 attatttctt attatttcct gttattccta atctatcatt catatctttt atcatttttc
     5281 taaaaccact acctatatca tttccaacat cttcagttcc atgaactaat tttataaagt
     5341 cattttgacc ttttttcata tcttcatcag ataccttatc taatttttct attctcttat
     5401 ctatatcttt tactaatctt tccccttcag cgattaggtc atcattcatt ttctttaact
     5461 ctgtatcttc tgttttacca tcttttttaa gagatgttac aaatgattgt attttatctc
     5521 tactatcttt atatgcagcc tttacatcac ttagatattt ttcatttcct ggatattctt
     5581 tatctttaaa ctgattattt atactttctg gagtatcata aatagaatat ctatttagac
     5641 ctgctatata atcattatag cttgttgaat aataattcat atatccttca ttattattta
     5701 cagattcatt aggatttttt cctgtatttt cacttggttt ttcattattt ttagtacatg
     5761 cagttatacc tatagctaat actagtgcta atcctacaaa taaagttctt tttaatttca
     5821 tttttatt
//
LOCUS       DAMJZE010000215         5793 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJZE010000215 DAMJZE010000000
VERSION     DAMJZE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.219"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(50..334)
                     /locus_tag="PC541_003473"
     CDS             complement(50..334)
                     /locus_tag="PC541_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840894.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            541..1359
                     /locus_tag="PC541_003474"
     CDS             541..1359
                     /locus_tag="PC541_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2840895.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1492..3612)
                     /gene="feoB"
                     /locus_tag="PC541_003475"
     CDS             complement(1492..3612)
                     /gene="feoB"
                     /locus_tag="PC541_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2840896.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3638..3865)
                     /locus_tag="PC541_003476"
     CDS             complement(3638..3865)
                     /locus_tag="PC541_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2840897.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4192..4632)
                     /locus_tag="PC541_003477"
     CDS             complement(4192..4632)
                     /locus_tag="PC541_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2840898.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4784..5773
                     /locus_tag="PC541_003478"
     CDS             4784..5773
                     /locus_tag="PC541_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2840899.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
BASE COUNT         2133 a          773 c          759 g         2128 t
ORIGIN      
        1 ctttttatta ttaattttat cagtgtttta ttataaatag cttacataat tacacatttt
       61 ttctgattaa ataatatgat gctattgtta taatagatac taatgctaat gatgttattg
      121 cagtatctaa tcttcttatt aataaactca tttctatata ttcaagttct attaaatact
      181 gataaaatat ttctagttta acagcttttt ctctatctat atttccgtac tttaactcta
      241 atttatctaa agatttttta ttacaataat taactttatg tttaattaaa tatcttattg
      301 tagcacctat tacaattttc actctaacaa gtataaatat aatattccat ccaaaagtta
      361 agaggggata tcttttttat gatgggaaag ggtaaaatat ttataactga catctaactg
      421 atatttaagc tttaaaatca aaaccatatt gattttctaa aataataact acttgattta
      481 gtaaataata aagaatatag taactatata gacaacaaaa agaaaaggag tgtataaccc
      541 ttggaaaaac agtattttac aacaggtgaa tttgctaaat tgtgtggtat atctaagcag
      601 acattaattt tctatgacaa aataggtatt ttttctcctg aatataaaga taaaaataat
      661 tatagatatt actcagttta tcaatatgat acacttgata tattacaatc attaagagaa
      721 ataggaatga gtttagagga aataaaagag tatatacaaa atagaactcc ccagctttgt
      781 gtgaaaatgc ttaaagaaga agaaaaaaag ataagagaaa aaataaaaaa actaagaaag
      841 ataagttcta agatgcaaaa tagaataaat ataacagttg aaggaatttc taaaatgaat
      901 agtgaagaaa tttatatatc caaaaagttt gaagaatatt tagttctatc aagcgatttg
      961 agtaatatga gtaatagtga ttttatgatg gaattaatag attttaccaa ttattgtaaa
     1021 tcgaaaaatt tgtatcaagg atatgagttg ggagtaatag taagtaatga aaatataaaa
     1081 aatggggact atttaagtat atcaagtttt tatttgaaga tagataaaaa aataaaggat
     1141 aaaaagctat atgtaaagcc agaaggaatg tatgcttgca taaaacatat tggaaaatat
     1201 gaggattcat ataaatctta cgaaaaattg aaaaaatata tatatgaaaa tgaatataaa
     1261 attgttggaa attcgtatga ggagagtata cttgactttt tttgtgaaag taatgaagat
     1321 aattatatga ctgaaatttc aatccaagta tccagataag aattaaaata gagatatatt
     1381 caaatgttaa gtaattttac ttatcattga atatatctct atgctttata ataaagtata
     1441 taaattttta aatggtttct ctaatatatg tctactgtca gttaatacta attatataaa
     1501 taaattgtaa actgtagttg ttaaaaaaca aacaacaaaa gcaagtatag taggcattaa
     1561 gaatcctaag gcagtccatt ttaaactacc agtttctttt tttattgtta aaagtgttgt
     1621 tgcacaaggc caatgcagta aactgaacag cataacattt aaagctgtca aataagtcca
     1681 tccatgttct ctaagaactt gacctaaagc actaaagcta tctaattcta tcatagaacc
     1741 agttgctagg taagccatta aaagtatagg tacaactatc tcatttgctg ggaatccaag
     1801 aataaaagca agtaatataa aaccatcaag accaattaat ttagctaaag ggtctaaaaa
     1861 gtttgcaaca tgtgataata tacttaaatc tcctatatat atatttgcaa atatccaagt
     1921 tatgacacca gcaggtatgg ctaccattac agcccttcct aaaacaaata ttgttctatc
     1981 tattatagag gtatataaag ttcttcctat ttgaggaact ctataaggag gaagttctaa
     2041 tgtaaatgta gacggaactc cttttaatag cgtcttagaa agagtgtaag aaaccaacaa
     2101 tgttattata acacctagaa ttatcagtaa agttatgcaa agtgcagttg ctacgcttga
     2161 tacgaaagag ttggttataa ctgagctaaa aaatattgta gatatagcta tcaaagtagg
     2221 aaaacgacca ttacaaggaa caaagttgtt tgtaagtata gctataagtc tttcccttgg
     2281 agagtcaatt atcctacaac caataactcc agctgcatta catccaaatc ccatacacat
     2341 agtcaaacac tgttttccat gtgcacaagc ttttttaaat aggtggtcta agttaaatgc
     2401 aactcttggc aaatatccaa aatcctcaag tagggtaaat aatggaaaaa atattgccat
     2461 aggaggtagc attacagaga ttacccatgc taatgttctg tataatccta aaaccaacat
     2521 atcatttaac catgatggac aattaatact atttaatata cccgaaatac ttggctcaaa
     2581 tccgagtaat aaattagata ataaagtgga tggatagtta gctccttcta tagtaatcca
     2641 caatatagta cctaataata agagcattat tggaagccca aatattttag atgttaatat
     2701 tttatctact ttttcttctc tatcagttga ttttttagca acattagaac aacactcatc
     2761 actcagtttt ttagcataat catagttgat tttagtaaac tcatctctta ttttctgttt
     2821 atttatattg tctggaatct ttttctttac ttcattaata gcatctatag aatttttgtc
     2881 tatataatta ctcatagatt caaatatact ttcatcgcca tcaataagtc taagtcccaa
     2941 ccatctagaa tttatacctg gtataatatt atccaattct ggttgaattg attttaccac
     3001 attctctata ttttcattgt atctaacagg tttattattt aatttatatt tgtcaaaaga
     3061 tacatcattt aaagtatcca atagttcatc cataccagag ccatttcttg cagcagttaa
     3121 aataactgga ataccaagag aatcctctaa aagtttttta tcaatagtaa tgcctttttt
     3181 tctagcttca tctattaaat taatacacaa aataacttta tctgtaagtt ccatcacttg
     3241 aaatactaaa tttaaatttc tttcaagaca tgtagcatca catactacta ttactgcatc
     3301 tggattacca aaacatataa aatcacgagc tacaatttct tcttgtgata aggcaaataa
     3361 agaatatgtt cctggtaaat ctattaatgc atattcggta tttttatatt taaaattacc
     3421 acgagctgta gctacagttt ttcctggcca gtttccagta tgctgtctaa gtcctgtaag
     3481 atgattaaat acagtacttt tacctgtatt tggattgcca gccaatgcaa ttacaaactg
     3541 gtcttcctta ttgtctatat caaacatgtc ttttaaagaa ctcattttag ttgagttatg
     3601 tgtcaacccc atattaatat cctcctgcta gaaattttta aatgtcagaa actaatatta
     3661 agctactttc ttcttgtcgt agagctatct tgctacctct gattttatag acagttaaat
     3721 tattttttgg accttttcta actacatcta taacagctcc tctagtaaga ccaagcgcta
     3781 acattctttc tcttaaatta ccactagata atatatcttc aactttgaca gtttttttca
     3841 cttgaatatc gttaaggttt ttcataatag cacctccatg cttgaaattt agcctgggct
     3901 aatcttctta ttttaaacta tgcattatat tattaccttg ttacttttac aaaaaaaaga
     3961 taggcaagat gcctatcata caaagttggg tataattctt acttttgggg aaagtagaat
     4021 atagaataaa aattaacact ttgaatccat ataattattg aattttgata ataaaaaagt
     4081 cagaaagcaa aagccttctg actctatgta aggtattttc aaatacctaa atatcaattc
     4141 aatatttaat ttatacttaa attatatatt aacaatatta gcttgtcaac actataatct
     4201 cattttaata tgttcaatac ctgcttcgtc gtatacttct gagatttctt taaaaccaca
     4261 agataagtaa aggttcttta tataagtttg tgctgataag gttatattat tttcgtgtaa
     4321 gttaactttt ataaaatcta tagcacaatc catcatttgt tttgctaatc cttttcttct
     4381 atgactagaa agaactaaaa ctctaccaat ggaaacatca ttataagagc tgtattcttt
     4441 aggaattatt ctagcgtaag cacaaatcaa tccattttct tttaaaaata tgtgataaga
     4501 gtttttatct atatcatcat aatcgtttaa tgaaaatatc ttctgttcac aagcaaatac
     4561 ctcatatctt gattttgcta tttcataaaa ttcatctaaa ctcaaatcat taaagtgttt
     4621 aattttaaac atagtaatta cctcctagca tcttaaaaaa ctgaatatta agatacatta
     4681 taccattttt aacataaata cctttattaa aatttatatt tattaaaaat atttttaaaa
     4741 agtgatatac tatattaaaa acaaaacttt agttagaaaa tttatgattt atggaggcag
     4801 tgttttgaat actactaaaa aattaactga agcagcttta ttgtcatctt tattcatagt
     4861 agtaacaatt atagctgtga gtactggatt tggatatgct atttacttag attttatagt
     4921 gccagtattt ttttgtataa tctgcttgaa atgtgattta aagtatacca ttttatctgg
     4981 aattacttcg cttttaataa tttctctggt acttggaaat ctaggaactg ctatttgggc
     5041 aagtcagagt gtattacttg gtataatgtg tggctatcta ataaataagc ctacaatggt
     5101 tatggatgat ttagtatatg gttcagtatt tgggcttatt gtaatggttt ttattgatat
     5161 atatgcttca acattaatag gttatagttt tatgaaagaa tttcaaggat atagcaaatg
     5221 gatttatttt aatgggtata caaattttat ttattattta atgatagcat tgtttccttt
     5281 tggtatggtg ttttgcatat atttattaag tttaatttta gggaataaat tgcatatttt
     5341 agattctaat tctctaaaaa aatttttaat atttaagaat tttagaagtt taaaccaatt
     5401 tttatgttgc tcaaaaaaag ctttttacat atgtgtttct tatctagcca tttttgaagt
     5461 tctaaaactg ttgaatgtta aagttgattc tgtttattta aaaacaatct ttatatcaat
     5521 aacctatata agtctctatt ttatcattag agactcatgt atgtcactac aaaactttat
     5581 aatagctaag tttagaaaat tattatatgc tagaatttta tttttgataa taattttatt
     5641 gctgtttttt atatttgatg taacagttat tggtttaata attataaacg catttttaaa
     5701 taagaaaata aatataagat tgagtcagag taatatagtc aataaacaaa taaatctttt
     5761 gattgaaaaa taaattttgg gtattggtaa aaa
//
LOCUS       DAMJZE010000216         5717 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJZE010000216 DAMJZE010000000
VERSION     DAMJZE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.220"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            235..417
                     /locus_tag="PC541_003479"
     CDS             235..417
                     /locus_tag="PC541_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840900.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1244..2158
                     /locus_tag="PC541_003480"
     CDS             1244..2158
                     /locus_tag="PC541_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2840901.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2548..3195
                     /locus_tag="PC541_003481"
     CDS             2548..3195
                     /locus_tag="PC541_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2840902.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3362..3931)
                     /locus_tag="PC541_003482"
     CDS             complement(3362..3931)
                     /locus_tag="PC541_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840903.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3936..4835)
                     /locus_tag="PC541_003483"
     CDS             complement(3936..4835)
                     /locus_tag="PC541_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2840904.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4846..4995)
                     /locus_tag="PC541_003484"
     CDS             complement(4846..4995)
                     /locus_tag="PC541_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840905.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4988..5572)
                     /locus_tag="PC541_003485"
     CDS             complement(4988..5572)
                     /locus_tag="PC541_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2840906.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         2092 a          860 c          662 g         2103 t
ORIGIN      
        1 tattaattta tattaaatat ctactaactc aaatattcat aattaaactt taaactggat
       61 aaattttctt ttaagaatga aagtttatcc agttttgcga ttataataac cagttttaca
      121 tatccacttg taaaatatat ctaagcaata tatcatacta tatagattta tgagtgaaat
      181 aatatctgct atttactgaa tatttttatt tttcgttttt tagggggatt agttgtgttt
      241 aaagataaga ttgatgaatg tgttcatata atgactgcat atattgctag tttaaaagaa
      301 tactattcat tcatagaaac tcaaattggt gattttatta aaaaatatgg agaagatgtt
      361 gtagagttat gtctccatag agtgatgatt ctactatgcg aatgtggttt ggcataaaat
      421 taaatttatt aaaatgggta agaagaatcc tatataatat tcttcttacc cattttaata
      481 tatcaatttc atagcttatc gtttaaaata ttttacatcc attactagac aactttacat
      541 tctcttttta attctagtat atttaaatca ttttagttgc tccttattta ttcttattac
      601 ttgttttatt tataaaataa ttctttttaa taatgttttt tgttgatatt cctatcacac
      661 atccaattat ttatcattgt cttgaaattg tttgcatact taatttaatc caattgaaac
      721 accccagcat agaatcccct gtaaaataga agttggatta aatcctaaca tctatacaga
      781 aaatactact ccaagcacaa gcaatataat ggaatatatt tatcacttaa ttttttatac
      841 tttttaaatc ccatacctaa aacattaagt gcagctatta gaattaataa ttgttgtggt
      901 ataaaattat gcttgcttca tatttattca ttttttgcta taaaagttct tgttttacaa
      961 aaaacatttg ttttgtatca aaataatact tataaattac ataattctca taaaaatttc
     1021 tagtattact tgtactctta ataaataata gccaacaagt accttaaatc attatatcta
     1081 tatctaataa gaaacacagt aagataatct tttcgcccat aacttaataa aaactgttga
     1141 tattagtagc aattgaattt agttaattta tttataatat acataaattt aaatattacg
     1201 aataaagtat tataaatagg atactttcta aggggaggaa attatgatag ataatcaaaa
     1261 atatgttatt ttatcactag aattacattt atttttttca agaattatga aagagcatgc
     1321 tcttttttta gaagcaggat tcacaaataa aaattataat cttgctatgg aagctgacca
     1381 ctataaaaag caatttgaag atttattatc atacactgtt agtgctagta atggtataat
     1441 tagacctgat atattatatt cagaagagct tgtaactact ctcacatcag ttgcagaaca
     1501 aaaaacagaa gagtttacag ggatagaaat aaacaaaaac atcactacaa gagaattaaa
     1561 tctacaaagt ggtgtaaacc cacaagttgg tcaagactta gtgaactatg tagctcaact
     1621 taactctgat gcaataagat tacttgatgg gcttattaat tttaaagaaa gagtcttaga
     1681 tggtgtacta tcatgtacta tatttacatc aaactaccct ctacttcttg aacatataat
     1741 acatgaagca aatttatatc gttcttatgt agttgacctt gaaaataaaa tagatattga
     1801 gtcaaaaaac gctaaagaaa tagaattatt ctgggaccat attatgatgg aacatgctct
     1861 gtttatgaga ggattactag acccctcaga aggtgaacta ataaatactt caaatgattt
     1921 tgctataaaa tttaatgaat taattgaaaa aacaaacgaa atgactgatt ctaatatcaa
     1981 gaacattaca gaagaaactc taaatgaaac tgttgagttt aaagatttta aagaagcagg
     2041 agcatcagga atagaacagt gtaagataaa atctataata ttaccacttt tagcagacca
     2101 tgttttaaga gaggcaaatc attatattag aatattggag agttataaaa acatgtaatt
     2161 aaaaattact tttaaaataa tctttgcata tttagataaa ataaaggtac agttaactgt
     2221 acctttattt tttataaact tataaaattt aaacaagtta ttcataaaat aaaataataa
     2281 tcatctataa cccacaactt tattttatat taaataaaaa gtattagtgg ttatgtttta
     2341 taagtatttc tttattcagt tatttattaa aaactaatag taaatcttat actaatcgca
     2401 tatttatgtt ataactatat tgtaactatt ttcgtaatct taggaaaaga ttacttataa
     2461 aattaacctt atacaataat atatcgtata atcttaatta gcataatagt tacaatatta
     2521 aaatttaaat atgggagtga ttttagtatg ataagacaaa caccattaga gaaacgatat
     2581 gacaagctag gtcctcatat tgtaacagca ctcaaaaaac gttattttga tgcttattac
     2641 tgtaaaacta gaaatgaagc tttgcaacaa attttagatt taattcctca aaatcatgta
     2701 gtatcatggg gaggatctga aacacttaaa gaaatgggcg tacaaactgc tgttagagag
     2761 aaaggattta aagttattga tagagattta gcaaaaactc cagaagaaag aactgaaatt
     2821 atgcgtcaag cacttctatg cgatactttt ttgatgagta gcaatgcaat tagtgaagat
     2881 ggacaactct ttaatattga tggaaatggc aatcgtgtag ctgcaatgat atttggtcca
     2941 aaaaatgtta tagttgtagc tggtatgaat aaaattgtaa aaaacattga tgatgcaata
     3001 caaagaacta gaacaattgc atctccagca gttgttcagc gttttcaaga tgtcaataca
     3061 ccatgctata ttaatggaag ttgtatagat tgcacaagtc ctgaaagcat ttgtgcatac
     3121 atggttacta caagaatcag tcgtccagca aaaaaaataa aggtaatatt ggtaggagaa
     3181 aatcttggtc tataaaatat acatacatgt aaatagctct ataaataata aaatcatatg
     3241 aaataactta tttctaaaag tggtagaata aaagcttcta caagactttc attctgccac
     3301 ttttatattc ttttaatttg tgtttctata ttattaattg ctccaatttt catatatatt
     3361 actaactatt tttattttca taattttttt gttcttcatc aagctgttta taataatctt
     3421 tattatagtc agctagataa tctataccac ctcctgtaat cacatttaat aatttcattg
     3481 cagattcttg attaacttta caaacataat tatcaccaga tttaacaagc tctaatgtaa
     3541 tattaaattc ttttttcttt gcagaatcaa tatctttcat agttggattt ggcatcattt
     3601 ctcctagttt aggaggattc ttttttaaaa tagaaaccat atcagtattg ttaattttta
     3661 aatctacagt cactttatcc ttattatttt ttatatctga aatttcccaa gtcatatttt
     3721 tagaatacat tttaaatcct tctttatctt cgtatataag attacctaac cctgattttg
     3781 ggaaatactt atcaacagta tctgcatctt gtgtttttat tgaattaaac atactatcca
     3841 cttcattttt tatcttatta tcattagtac aaccagctaa caccagtact gatattgcaa
     3901 acattataat acatagaata aatttcttca ttaaattata tccccctata cttttttgca
     3961 caaatattat tctctttttt atcttttgaa aaagttgtag taattgcatt ttgtggacaa
     4021 acttttatac attcaccaca cctaatacag tctggactat ttacatcttt atatattggt
     4081 atatcaagct tacattttgt ttgacatgct gtacaatttg tacatttatc tttatcaatt
     4141 ttaaatctat atattgaaat aggattaaaa aaaccataga ttgctcctaa agggcatata
     4201 tacttgcaaa atggcctata agtaatgatt gacatcaata atatcaataa caaaatactt
     4261 aacttccagc taaataaaaa tcctattgat tctcttaaat tctcatttcc taaaataagt
     4321 ggtattcctc ccaaaagtgt tcctgaaggg caaatccatt tacaaaacca aggatttcca
     4381 ccccctgcta catttacaat agcaactggc aaaataatta caaatattat taagactata
     4441 tactttagat attttagata tttatcacca taaacttttt ttatctttac tggaaagggt
     4501 actttatgta gtaaatcttg tacaagtcca aagggacaaa gccaaccaca aacaaatctg
     4561 ccaaaaaaag caccaaatgc cattaaaaaa ccaacaacat aaaatgagaa tttatactct
     4621 ctgcttgata aaactgcttg taatgaacct attggacatg ctccaagtgc acctggacat
     4681 gaataacaat tcaatccagg tacacataat tttttacttt ctcctccata tatagttcct
     4741 tctaaaaatc catttacata tccatttgtt aatgctgtaa atgcaacttg agtaataagt
     4801 cttatattat tatttacttt agataataac ttcattcctc ttctcctaac caataccaat
     4861 acactcaaga caaatcataa ttgctttagt taatactgta tttgtttctc cacgatatgt
     4921 acctaatgca ataaatataa tactaattgc aataatagaa atgccttttt tattatcttt
     4981 aataaagtta ctcattggct tttaccattt ttagtctttc atctataatt tttttccaat
     5041 cttccttact atttgcacct acaaccttct cacccacaaa gtttcctttc ttgtctacaa
     5101 aataagttgt tggaaactcc ataactcttt taaaatcagt ttttttaagt tcttcagata
     5161 cagtcaattg ttggtaagtt gcatctgact ttttcataat gtctttaact aaatttttct
     5221 cttcatttga aagaccagtt ctcatatcag ttttatcaac ttctacaact aatcccttta
     5281 tagctacatt ggaattatta ctctcatatt cttttgataa tgcttcaagc tctggcattt
     5341 cacccacaca tggtccacac caagtagtcc agacattaat aagagtaagt tctttatttg
     5401 aaaatatctc ttttgttacc ttatttccat caacatcctc cgtatctaaa ttactaaacc
     5461 attcactatt attcttgttt ttttctactg gcttagattc ttttttgcta catccagaaa
     5521 tcatacctaa aacacaaata catcctaaaa ctactatgaa aaaccttttc atatctcaca
     5581 ctcctagaat ttatatagta tataaaatcg ctgcttgaaa taaaaataac ttatcttcta
     5641 tattaaagta attatattta ataaataaga gctaatttaa aaaaagatat acagttaaaa
     5701 tctatttctt ttcagac
//
LOCUS       DAMJZE010000217         5630 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJZE010000217 DAMJZE010000000
VERSION     DAMJZE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.221"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            256..1083
                     /locus_tag="PC541_003486"
     CDS             256..1083
                     /locus_tag="PC541_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2840907.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2108..2572
                     /locus_tag="PC541_003487"
     CDS             2108..2572
                     /locus_tag="PC541_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2840908.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2839..3483)
                     /locus_tag="PC541_003488"
     CDS             complement(2839..3483)
                     /locus_tag="PC541_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840909.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3413..4702)
                     /locus_tag="PC541_003489"
     CDS             complement(3413..4702)
                     /locus_tag="PC541_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2840910.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4699..5571)
                     /locus_tag="PC541_003490"
     CDS             complement(4699..5571)
                     /locus_tag="PC541_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2840911.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2037 a          781 c          699 g         2113 t
ORIGIN      
        1 atcttctatc ttctaaataa ttttaacatg cccataaaat ataataccca caaatgataa
       61 ataatactca aaatgtctat tgaaataact atacatcctt aaataactta acttaaatct
      121 aaacatttca aaaacttctt aaaaatatgt aaaaatttga tgataaagta gcgatttttt
      181 caaaaaaaat gtaatataga gttatatctt ataatatttt agagataaat tttatcatag
      241 tggggagaaa tttgtatgga tttaagcaaa aaagtaaaaa ttttagaaaa tgcatcagat
      301 ggcaaaagcg aatttgagat attagaattt gaaaaacttg agggagcttc tagcccatta
      361 atggcaatgg gattatattt tgctagagaa gctggtttga aagctagaca aattaagata
      421 aaattaaata attcatcaat aaaaacagaa gctggtgcat tatactatta taaaggaaat
      481 atatcttctg aaactaagat tggtggtgtt ggaggtcttt ttaaaaaagg aatatcagga
      541 gcacttacta atgaatcaat gatgaaacct gtatacaaag gtactggaga agtttatttg
      601 gaaccttctt ttagacatta tttatttatg agtcttgata atgattctat aattgttgat
      661 aaaggattat tctattgttg ctctgataca atagaagtaa aagcatttac acaaaagaac
      721 ttctcatctg ctttacttgg tggtgaaggc ttcttccaaa tagaattaag tggtactggt
      781 gtaatcgttc tggaatgtgt tgtacctcaa agtgaaattg tagagtatga attgaaaaat
      841 ggtgaagaat taaaggtaga tggtaacttt gcaatagcaa gaacatctgg tgttaatttt
      901 tctgttacaa aatcagataa atctttaatt ggttctgctt taaatggtga aggtttctta
      961 aatacattta ctggtaatgg tacagtttgg ctagctccaa ctcaagtaat gtatgaaaaa
     1021 ctaagttttg gagcaatgcc tactaatgat aatatgaata ataaagtttc taagcaaaaa
     1081 tagttgtttt ataaatattt attaaatcat tattgatttt ataaagtcac tataaatttt
     1141 attagattat tattataaaa tcagagtgtg gaaatgttta tgtgtattct ttaaataaaa
     1201 atttccacat tctgtaaggt tattatactt gatatataaa attcaattta ggaacatagt
     1261 ctaacattta taaacatatt aaggtaatga tttctcttgg taattattct catatcttat
     1321 tctttgatta tatttatctt attcctagcc cttatgtcat attatatttc ttatcttatt
     1381 acttataatt agcattaact tatctaaaat cttgtatttt tttaatagta aagtttacat
     1441 ctatattaaa aagtttacat ctatattaat ttcataaaac aatcaaggta gtcaagagat
     1501 tcaactttac ttgattttaa aaatttaagt gaatttgttc ttatacacat ctttttaaaa
     1561 ttatatctgt agaaaatatc atttagagct ataattaaat taacttatta aattttagaa
     1621 aatttattaa aaaatataag ttaaattaat atttttaaag attctttttt gtaactacta
     1681 gaatagtaat ttagtagtta ttgtatctct aaagtttaag catggataga tatacatttg
     1741 ataatttgcc tataatttac ctactacttg gattattgga gcaaatacaa tatattaata
     1801 ataagttcct aaaaccaata aattaactaa tacacataaa gatattgatg ttgattatac
     1861 aactataaga aacggattta acagaattta atcaagctaa gaaattatat atatatcgtg
     1921 atgtttttga attagaaggt aattcaaaat tagtttatag tcttctgtca atccaataaa
     1981 aagtacaggg tacaagaatt gttagtaaaa tttttaaaga ttttatggat aaatataaat
     2041 agttttactt attttctaaa actttaaaat accattaatt accttaaata ttttaatagg
     2101 aggacatatg aatttaaata aagttaaatt tactgaagaa catgctattg aagttactaa
     2161 ttggaaatat gaaggtaaat attcaatata caatctacct ccatgggagg aaattaaaaa
     2221 gaagaatttt tcactagcaa aagaagataa aagaaaaaat tttataagtt ttataaatga
     2281 caataaagaa ttgataggtt ttataaactt attagatgaa ggaagctctg tattctttgg
     2341 tattggtatg aaaccgaatt attgtggaat gggaattggt aaagaaataa ttggcctagg
     2401 attacaagaa tgtagaaata aatatagtac taaaccaatt gtattaaatg taagaacctg
     2461 gaacatgaga gctgtaaaat gttatgagtc tcaaggattt aaaatagttg agactaaggt
     2521 acaaaaaacg catttaggag atggagaatt ttttgttatg aggtatcaat agtttataac
     2581 aatagtctta ttgttaatac aacaattaaa atgaaaaaat acatatgtgt aggtcatagc
     2641 aataagtgtc tttgaaatat aaatttgaga gcttttgata aaattttgta tacatatata
     2701 ttttatacta cggatatata aatctatcta catatataaa gagattgtaa gtaggtttgt
     2761 gtatataaaa atagatttat atttcataaa tgtacttttt atttgtatta cctttatttt
     2821 atctaagctc tcattttatt atctaatctt cctttttact ggttttatta agagagatgc
     2881 aattatcaac acaacacttc caagtatcag ccaatatttc tcatcaaaac taccttgact
     2941 catagacatt ataaaaaatt ttccaacata cttgccttta gtcatcatat ttaaaattaa
     3001 atatcctata gatatcatat aacctatgat tgtattatta aataaatttg agaaaaacat
     3061 tcctaaactt cctataaaca tagctgatgc gaaacttcca ataatatact caactactgg
     3121 aaaattacat ccaagatata ttaaaacatt tacaaaacct atgattaata ccactatacc
     3181 tataattgac atcagtatcc taattaattg tacaataact tgagataatg cttttgaaga
     3241 tactatctca tcaatttctt ttgattgctc tagcgaaaat acaggtacca acattactat
     3301 ccctattatt gcaacataat tttctaaaca tatagctgtt gaaattctgt ctaaattagc
     3361 tactccttgt attagaggaa ccatacatag caatataatt gaacatatta aattagttaa
     3421 agtgcttaat ttgaagtttt cttttattag aactaaacag ttttgaaaac tttgcccaaa
     3481 catcaagatt cccccttctt ttttgctcat ataagaatat agctaacaaa acaagcacca
     3541 atgctcccaa gcttatcaca attctattaa tcataatttg attgaagttt tgtataataa
     3601 tatccccatt taatacacta ttatgtctta tcattagccc cattgttgaa taatcacctt
     3661 tgagtcctgt agaatttata ctgtaaagcc accaaattaa ttgcacaata attgctactg
     3721 gtgtatctgt taatatagta atactcattc ctacagctgt aactatcata agagttggta
     3781 gtatccacca taatgtatat tttataaatg ctaagttatc aatagatata cctttatctt
     3841 tagcaaagaa aatcaaagga agtaaatctt tcatacctaa aattaataca ggcaatatca
     3901 tcataaagac aagtgcaaaa tatcttgata aaactatttt gcatgaagat acttgttttg
     3961 aataaataat ctctctcatt cttgaacgct tatctttaag tgacataaaa actgccacaa
     4021 atactggaaa tatacttaaa actataccta tataatcgca aaaaattctt gcatacccat
     4081 tactaacttt atctttttgt attgtatagt tgtaattctt catagcttca tcataggtaa
     4141 ctggaactac tccaaatctt tgtaacttgt caaaagcata tgcagaccct cctcctaaaa
     4201 tgtcgtctac tctttgcatt atttcttcaa atctttcaag agatacttta gaataatcta
     4261 tttttatact ttcatcagtt ttccaaagtg aatttatttc gctaatactt ttaccagtta
     4321 tttcttctat atatttttct atggtcttcg ttttattttg gtctaatttt atatacttaa
     4381 ctacccctgt tggatacgtt gtatatgtgt ttttttgata ttctctaatt aggtttatag
     4441 ttgcatttgg aatgaccttt tctgttttca tagattcttt ttgaccatat ggatattgat
     4501 tgtgtacaga ataatattcc tcaatattct ctactttttg tggagcaact aatggatttc
     4561 catcattagg aatattttta cttacatcat ttgaaactga acttccaagt tgagaaatat
     4621 agaaaaaaac taaaactaca ataaaaatat aatataccat actctttaat atatttttac
     4681 attcttttat aaatagattc atatattatc tcctcccatt ataagcatat agccatcttc
     4741 aatagttgga ttacaattta ctgctgtctc aaaaggttta tcatctgata taatcctaag
     4801 tatagccttt ccaccatgag tcaacattcc tataactata aatctacttt ttatattttc
     4861 aatgtctttt ttatctactt caatactata tactttccct tcagctagtt ttgctaatgc
     4921 ttctgttgag ccattgtata tgacttttcc actattcaat atagctacgt tttcacatgt
     4981 agcttctata tctccaacaa tatgtgtaga taaaataacg attctatcat ttgcaatttc
     5041 gcatagtaaa tttctaaatc ttatacgttc ttctgggtca agacctgctg tcggctcatc
     5101 tactattaaa actctgggat tatgaattaa tgcttgtgct atcccaagcc ttctattcat
     5161 acctccagat aaagatttta ctttcacatt ttgataatta gtaaggttta ctttgcttaa
     5221 taactttgct ataatttttc ttctttttct tagtggtata tccgataaaa ctgctaaata
     5281 atccattgct ttatatactg tcatgttagg atacattgaa aaatcttgtg gcaaataacc
     5341 aaccatattt ctaatttcct tagtattctc aataggaact ccacacatat gtattgaacc
     5401 ttctgttttt gataataaag tggctaaaat tctcataaat gtggtttttc ctgctccatt
     5461 tcttcctaaa agaccaaaca taccactgtc gatttcaaga tttatattat caagagcttg
     5521 ttttttccca aatcttttac taagattgga tactgctatt ttcatttcca tatataaata
     5581 cacccccaga aacaatttta tagaatttta tttttataaa tttttactgt
//
LOCUS       DAMJZE010000218         5615 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJZE010000218 DAMJZE010000000
VERSION     DAMJZE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.222"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            132..632
                     /locus_tag="PC541_003491"
     CDS             132..632
                     /locus_tag="PC541_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2840912.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="PC541_003492"
     CDS             689..1675
                     /locus_tag="PC541_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2840913.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="PC541_003493"
     CDS             1725..2447
                     /locus_tag="PC541_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2840914.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="PC541_003494"
     CDS             2476..4410
                     /locus_tag="PC541_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2840915.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..>5615
                     /locus_tag="PC541_003495"
     CDS             4403..>5615
                     /locus_tag="PC541_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2840916.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLD"
BASE COUNT         2343 a          428 c          970 g         1874 t
ORIGIN      
        1 aatgaattgg gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttaaaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttttggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaaa
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caggtaggtt
      541 ttggagaatt tgtagtagta acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtgataaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagctg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaagg aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttagag aagaaagcat aattgcactt
     2581 ggatttgata taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagtt catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagc ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggagggtgga
     3241 tatttttttc ctgttagatg gatagagtat gaaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca gatactatac aggttactta
     3481 gattataaaa ataatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggcc ttaagtctga tggtgaaggc aattttaagc cagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gaccatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataaggata ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 gtatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 acttagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tatagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctgttggatt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttctac ttttaaattt
     5161 gtctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaactg gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taagtttata gaagcaaaaa ctgtgaggtt agatg
//
LOCUS       DAMJZE010000219         5610 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJZE010000219 DAMJZE010000000
VERSION     DAMJZE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.223"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            172..1218
                     /gene="recA"
                     /locus_tag="PC541_003496"
     CDS             172..1218
                     /gene="recA"
                     /locus_tag="PC541_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2840917.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            1394..2935
                     /gene="rny"
                     /locus_tag="PC541_003497"
     CDS             1394..2935
                     /gene="rny"
                     /locus_tag="PC541_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2840918.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            3249..5585
                     /locus_tag="PC541_003498"
     CDS             3249..5585
                     /locus_tag="PC541_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2840919.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT         2251 a          602 c         1035 g         1722 t
ORIGIN      
        1 atattttctt ttgtgtatat aattattaat aatggtttat aaataaaatg taatgaataa
       61 aaatataatc attagtagcg tttttaaatt gactaggttt gatttatgtt atataattaa
      121 tatataacaa gagaacaaat gtttgtagat aaatgaaggg atggtataga gatgagtgta
      181 gatcaagaaa aattaaaagc gttgaatgaa gctttaggta aaattgaaaa agattttggt
      241 aaaggttcag taatgaaatt gggagaagca acatctatgt ctatagatgt tatatcaaca
      301 ggagcgattg gtttagacat agctattggt ataggaggtc tacctagagg gagaatagtt
      361 gaagtatatg gtccagaatc ttctggtaag actactgttg cgcttagttg tgtagcatca
      421 gctcaaaaag atggaggaat agctgcattt atagatgcag aacatgcact tgacccagta
      481 tatgcaaaag ctttgggtgt ggatgttgat aacctaataa tatctcaacc agatacaggt
      541 gaacaggctt tagagatagc agaggcattg ataagaagtg gagcgataga tataatagta
      601 atagactcag tagcagcatt agttccaaag gctgaaatag atggagatat gggtgattct
      661 catgtaggtt tacaagctag acttatgtca caagcactta gaaagttaac tggttcaatt
      721 aaaaaatcaa attgtgttgc tatatttata aaccagttaa gagagaaagt aggaataatg
      781 tttggaaacc cagaaactac tactggagga cgtgcactaa aattctattc atcagttaga
      841 ttggatgtta gaaaaataga tacaataaaa caaggtgata aagttatagg tagtagaact
      901 agagttaaag ttgttaaaaa caaagtagca ccaccattta agcaagctga atttgatata
      961 atgtatggag aagggatttc aaaaattgga gaccttttag atatagctgc tgatgtagat
     1021 atagttaaaa aatcaggttc atggtatagt tacaatgata ctaaacttgg acaaggaaga
     1081 gaaaatgtta aaaaattctt ggaagataat ttagatttaa ctactgaaat agatgagaaa
     1141 gttagagcat tttacaattt aaatgaagaa catgaagaat caggtacttc agtatcaaaa
     1201 gaaattgtag aagaataagt aaaaatacaa aaaaataact agtaattaaa gacatgccac
     1261 gctccctatt agcttgacac aatgttgaaa aaattataaa attaagtgag gataaaatat
     1321 ctaaatttaa ttttaattat taggaatatt ttttaatcat agtgatgaca ttttgggatt
     1381 attaacgaag gaggtggatg tcatagatag tgtagtaatg attgtaatag gtgcagctgt
     1441 tggtattata gctggatatt ttgttagaaa aaatatatct gaagcaaaaa ttggacaagc
     1501 tgaaaacttg gccaaagaaa taatagataa agcacaccac gactcagaaa cagtacaaaa
     1561 agaaaaatta ctagaagcta aagaagagat ccataagtgg agaacagaag ctgaaagaga
     1621 aaacagagaa agaagaacag aagtacaaaa gtatgaaaaa agagtagtac aaaaagaaga
     1681 agtcttagat agaaaattac aaaatttaga atctaaagaa gtaaacctaa gtgaaaaatt
     1741 aaaggtagtg gaaaagaaag aagaagaagt tgaagtaata aaaactcaac agttagaaaa
     1801 attagaaagt atatcaggaa ttacttctga taaggccaaa gaaatcatct taaccaatgc
     1861 ggagagagat gtaagacgtg aaatgtctat aatgataaaa gagatagaaa gtcaagctaa
     1921 agaagaagct gataagaaat caagagaaat tataggatat gcaattcaaa aatgtgcagc
     1981 agatcacgta gccgaaacta ctgtaacagt agtaagcttg cctaatgatg aaatgaaagg
     2041 tagaataata ggtagggaag gtagaaatat aaggacttta gaaaccttaa caggtataga
     2101 ccttataata gatgacactc cagaagccgt aattttatca ggatttgatc caataagaag
     2161 agaaattgca agaattgctc tagaaaaatt aatagcagat ggaagaatac atccagctag
     2221 aattgaagaa atggttgaaa aggcgagaaa agaagttgac aatattataa aagaatatgg
     2281 tgaacaagct gcatttgaaa ctagtgtaca tgggattcat ccggaattaa taagattatt
     2341 aggtagactt aactatagaa ctagttatgg acaaaatgta cttaaacatt ctatagaagt
     2401 tgctcatata gctggtataa tggctgctga aataggagca gatattagat tggctaagag
     2461 ggcaggttta ttacatgata taggtaaggc tgtagaccat gaaatggaag gtacacatgt
     2521 tgaaataggt atggatttac ttagacgtta taaagagtct aaagaagtta tacatgccat
     2581 gagtactcat catggagatt acgagcctca aacagtagag gctgtattag ttacagcagc
     2641 agatgcaata tcagctgcta gacctggtgc gagaagagaa actttagaag catatataaa
     2701 gagacttgaa aaattagaag aaatagctaa ttcatatgaa ggtgtagaaa aatcatttgc
     2761 tatacaagct ggtagagaaa taagaataat ggtaaaacca gaagctataa atgatgaaga
     2821 aatacatcta ttggctagag atatgactaa gaaaatagag gatgaacttg aatatcctgg
     2881 acaaataaaa gtaagcataa taagagaaac aagagctata gagtatgcga agtaaaagaa
     2941 ataatatcta aataataaga aaatattttt agaaatttaa atttgccaca agatatgata
     3001 atatatcttg tggcttttaa attgtttaag tttaatttta tataatgcaa aaataatcta
     3061 agtgctaaaa gtgctactta caaaagatta ataaaataaa gctttatata tgtattattt
     3121 atataattat ccaagtttgt ttgatataat gaagttgtaa aataaatata ttactacttg
     3181 gaatgcttgt acttaaataa gatgaaaata caatgacgct aaagtgagga ttagagagag
     3241 ggtacactat gaaaaaaaat acacttttac atacaaatat tatggtttgc attattatta
     3301 ctcttggttt tgtggctacc tctataattg gatatcaatc taataccaat atttttaaaa
     3361 atgatattga acatgtatct acattggctg ctgaaggtat ttattatcag atagataaac
     3421 tactttcaga acctataaat gtatctctta caatggcaaa tgacagtcta ttaaaaaatt
     3481 ttttagatgg tgagaaagaa catttaaatg atgaagaatt tatttataaa ttacaagatt
     3541 atctgaatgt gtatagaaat aaatattcat atgattctgt ttttttagta tcaactaaaa
     3601 ccaataatta ttatcatttt aatggacttg atagaaatct tagcgctaat aactcagaga
     3661 atcaatggta ttatactttc ttaaagaatg atgatgaata ctcattaaat gttgataatg
     3721 atgaagcaag taacaatagt attacagtct ttgtaaattg taagataaaa gatgataatg
     3781 gagcaactat gggaattata ggtgttgggt taaaggttaa ttcattacaa atgttgctta
     3841 aaggatataa tgacaaattt gatgttgttg cacgcttaat tgatgataaa gggtttgtac
     3901 aattagcagt agataaaacc ggtcatgaga atataaattt ttttgaaaat tctagtagcg
     3961 acttgtctaa ttcgaaacaa ttaatattaa acaacaaaaa ggaacaaaag agtttttggt
     4021 attcatctga aaaatcaaaa agttatattg tatgtcagta catacctagt ttaaaatggc
     4081 atcttgttct agaaaatgat tttactttaa tgattaaaca gcttcatctg caatttttta
     4141 aaggcattgc agttatagtt ttaattataa tatttgtact ttttactatt acacatgtgc
     4201 ttaaaaaata caaaaaccaa ataataaagc ttagtgtttc acagaaattg gaatatcaaa
     4261 aattgttgag caaatctact gaaggtcttt atgaaaatat ttttgaaatt gatattacta
     4321 acaataaagc tggttgtgaa gatgagaata ggtattttca tgaatttgga ttagggctta
     4381 ataccccata tgatgaagcc ttacatacaa tagctaaaaa ccaaatcaaa gaagaatatg
     4441 cacaaggtta tttagataca tttttacctg aaaatgtaat taaacgttat aatagcggaa
     4501 ttaataatat cagctatgat tttttaatta aaaaagatga agaaagttat cattggatta
     4561 gaattaatgc acgaattttc ttttggacat cagatgaatc tgttcgtatg atatcttatc
     4621 gtaaaaatat agatgaagaa aaaaatagag aattactact tctcgaacgt tcacaacgtg
     4681 attctcttac agggctttac aataaagcta caacagagga atttattcgt tcatttttaa
     4741 aaaatgaaag taatcaagat ttagaacacg tttttctaat tttcgatatt gataatttta
     4801 aaaatgtaaa tgataaatat ggtcatgtat ttggagactt tgttataagt gaatttgcct
     4861 tagaattaaa atctcaattt cgtgaggacg atattgtggg acgtattggt ggagatgagt
     4921 ttgtagtatt aataaaagac tttaataata ataactatgt gcttatagac aaattagagc
     4981 gtttttgttc taagattaat cagaaaaaat ttgttgagca agcagaattt tctattgcat
     5041 gtagtattgg tgtagcaatg tatcctgaac atggatatgt atactctgaa ctatatgaga
     5101 aagcagacca agcactttac tatgcaaaaa gccatgggaa aaattcttat catatatttt
     5161 cagaggattc tattgatagt atttcatttc atatagactc tagagatata aaagtattat
     5221 tagaaatggc tactgatggt gttgtcaaat ttgcttgtat ggaaaactta aaattacttt
     5281 atttcaacca aaaaatgctt gaatttactg gatattcagc tcaaactgtt acttctatag
     5341 gttttgatat tttttcgcct gtattacaag acgacttaca aatggtacta gatacattaa
     5401 gagaagcaat ttctaaaaga gagacttttg aaatagtttt tcgtttttgt catacagatg
     5461 gtcatatttt tcctgtaagg atgcaaggta agtttgtaag tgaattatat caaaatcgtt
     5521 atcctgtttt ttatgcgatt tatacagaca tatcaagtat caatacttta ctagaaaata
     5581 aatagtaaat actacagtaa aatctaaaaa
//
LOCUS       DAMJZE010000220         5449 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJZE010000220 DAMJZE010000000
VERSION     DAMJZE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.224"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            81..2312
                     /gene="pflB"
                     /locus_tag="PC541_003499"
     CDS             81..2312
                     /gene="pflB"
                     /locus_tag="PC541_003499"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2840920.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2472..3206
                     /gene="pflA"
                     /locus_tag="PC541_003500"
     CDS             2472..3206
                     /gene="pflA"
                     /locus_tag="PC541_003500"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2840921.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3328..4998)
                     /locus_tag="PC541_003501"
     CDS             complement(3328..4998)
                     /locus_tag="PC541_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2840922.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         1941 a          785 c          799 g         1924 t
ORIGIN      
        1 aatatataaa ataaattaag tatataaaaa ttaattttaa tagtattatt tatcatttaa
       61 atctaaggag gaaatactta atgaatgcat ggcaaggatt taaaacagga agatggacta
      121 aagaaataaa tgttagagag tttatacaat taaactattc tccatatgag ggaaatgact
      181 ctttcttagc tggtgccact gaaaatacta aaaaattatg ggacgaagca atggtcctat
      241 ttaaaaaaga aagagaaaat ggtggtactt tagatgttga tacaaaaact gtttctggaa
      301 tagctgcata tgctccagga tatttagata aagaattaga aacaatagtt gggttacaaa
      361 cggatgcccc tttaaaaaga gctgtcatgc catatggtgg tataaagatg gttgaaaatt
      421 catgtgaagc ttttggatat gaattagacc cagaaataaa agatatattt actaaataca
      481 gaaaaacaca caaccaagga gtttttgatg tttatactcc agaaatgaga gctgctagaa
      541 aatctggtat aataacaggt cttccagatg cttatggtag aggtagaata attggtgact
      601 acagaagagt tgctctatac ggtgttgatg ctttaataga agataaaaat gagcaaaaga
      661 aatcattaga agtttcttgc atggatgagg aagttatcag attaagagaa gaaataactg
      721 agcaaatatc agctttaaat gaacttaaaa aaatggctga atcttatgga tttgatatat
      781 caaaaccagc aactaactca aaagaagctg tacaatggtt atactttggt tatttaggtg
      841 ctgttaaaga ccaaaatggt gctgcaatgt ctttaggtag aacatctact ttcttagata
      901 tatactttga aagagattta aaagctggaa tcgtaacaga agaagaatta caagagtata
      961 tggaccattt tgttatgaaa ttaagaatgg ttaagttcct aagaactcct gattacaata
     1021 acctattctc tggagaccca acttgggtaa ctgagtgtat aggaggtatg ggaatagatg
     1081 gtagaacatt agttactaaa aactcattta gaatgttaaa tactctatat acattaggac
     1141 cttctccaga accaaactta acagttctat ggtcaactaa attacctcaa ggattcaaag
     1201 atttctgttc taaagtatct atagatacaa gttcagtaca gtatgaaaat gatgatttaa
     1261 tgagagcata ctggggagat gactatggta tagcttgttg tgtatctgca atgagaattg
     1321 gtaaacaaat gcagttcttt ggagctagag ttaacttagc taaaacttta ttatacgcta
     1381 taaatggtgg tgtagatgag aaatctggtg ttcaagttgg accaagattt gaacctataa
     1441 cttctgagta cttagattat gatgaagtta tgagtaaatt tgaaccattc actgattggt
     1501 tagcaacatt atatgtaaat actttaaatg taatacatta tatgcatgat aaatattctt
     1561 atgaagcatt agaaatggca ttacatgata gagatatatt tagaactatg gcttgtggta
     1621 tggctgggtt atcagtttgt gcagactcat tatctgctat aaaacatgct aaggttaaaa
     1681 ctattagaaa tgaacaagga atagctgttg attttgaaat agaaggagat tatccaaaat
     1741 acggaaataa tgatgataga gttgatagta tagctgtaga gttagttgaa agctttatga
     1801 ataaaataag aaagaataaa acttatagaa attcttatcc aactcaatct atacttacaa
     1861 taacttcaaa tgttgtttat ggtaagaaaa ctggtaatac accagatggt agaagagctg
     1921 gtgctccatt tgctcctggt gctaacccaa tgcatggtag agatactaat ggtgctttag
     1981 cttcattatc ttcagttgct aaattaccat atgaacatgc tcaagatggt atatctaata
     2041 ctttctctat agtacctggt gctttaggaa aagatatgac tgaaagaata aacaatcttt
     2101 ctgcaatgat ggatggatac ttcgctcaaa atgctcatca cttaaatgta aatgtatttg
     2161 atagagctac tttagaagat gctatggaac atccagaaga atatccacaa ttaactataa
     2221 gagtatctgg atatgcagtt aacttcataa aattaactaa agagcaacaa ttagatgtta
     2281 taaacagaac attccacggt aaaatggctt agtctttaaa tttttatact ttaactaaga
     2341 ttactactgt gtaaaaaata aaaataaaag gctatcttat atttgaatta tttctggtat
     2401 aagatggcct tttttagaga aatattataa ctattgataa ggttattaaa atatattgtg
     2461 aggtgaactc tatgattaag ggaaagatac attctataga aacgtttggt actgtagatg
     2521 gaccaggaat aagatatata ttattttttc aggggtgtcc tcttagatgc aaatactgtc
     2581 ataacaggga tacatgggat attaaatcag gaaaagaata cacagttgat gaaataataa
     2641 cggatgctct taagtatact tcttttatga agttttctgg tggaggtata actgcctctg
     2701 gtggtgaatc cactcttcaa ccagaatttt taagtgaatt atttaaaaaa gcaaaagaaa
     2761 atgatataca cacttgcttg gatacatctg gatttgtaga tatagaaact atagacccag
     2821 ttttagataa tattgatttg gttttacttg atttaaaaca tatggttgag gaaaaatcta
     2881 ttgatttgac tggtgttggt atggataagg cattaaaact tgctagacac ttagaatcaa
     2941 gaaatattcc tgtatggata agacatgttt tagttcctgg cattactgat gatatagata
     3001 atttagaaaa actaggtcaa tttgtagcta cattgaaaaa tgtagaaaga tttgaattgc
     3061 taccatacca ttctatggga atacacaaat gggaaagttt aggtatcgat tatgaactaa
     3121 aagatgttcc tgatgccact aaggaagatg tccaaaaagc tagtgaaata atctctaaat
     3181 ttggagtgaa agtatataat agttagtgta gtatattaat cctttataga agacataaca
     3241 aaatagggta aagtgtaaat ttttaataaa cttcaaaact tccactttgc cctattttta
     3301 tatactatat atctcatttt atatacatca ctttgatagc tgaaatttat aaaattcaga
     3361 ggcacttaca ggtcttccaa ataaaaaacc ctgaataata tctacaccta agctttttac
     3421 tatatcatac tcttcataag actctattcc ctcaacacaa acttttaaat ttataatatg
     3481 agctaattta ataatatatt caagaaaagt atattcataa ctattatatg taatattctt
     3541 aacaaaactt ctttcaattt ttattatatt aacagacagt tctttaaggt aatttaaaga
     3601 tgaatatcct gtaccaaagt catctattgc aatatatact cctataccct ttaaactaat
     3661 aaatttatca tttagtaaat ttatatctgg tatccaacaa ttctctgtta attccaaaat
     3721 taaaaattca ggtcttaatt gatactctac taaacattct actataaaat ctctaaaaaa
     3781 atcttccttt aactgaatat acgatacatt tacagatatt ttaaagtctg gatttatttt
     3841 atgccattct ttacactgtt ttacagcttc ttttataatc cacttcccaa ctggtataat
     3901 taaattactc tgttctagta ttggtataaa ttcaacagga gatacttctc cataagtact
     3961 cgaatgccat cttaaaagta cctctgcccc tattacctct tttgtaacag catttacctg
     4021 gggttgaaaa aacaactcaa aatcattaaa attattttca acgcactctc ttagtttttg
     4081 ttgcattgaa attaccttta gtttattttc atagagctct tgtgaaaaaa acttaattct
     4141 atttttacca ctaatttttg ctatatccaa tgcaatatca gcatgtttaa ataaatctaa
     4201 ataattatct ccatcttctg gatacatagc aacgcctgca gtcactgtgc aatagtactt
     4261 atttgattca atttcatgct gagtatttgt atacatctgt atcttttcat atattttctc
     4321 tatagtttct ttagtacaca tagggtaaaa aaatgcaaat tcatctccat ctaacctata
     4381 caatcttaca tcttttggaa gatattctaa tacttcagta gcaattgcac gcaatacttt
     4441 gtcaccataa gaatgtccat atttttcatt tatattttta aaattatcaa tatccataat
     4501 aaacatagca ccatttttat aaattttctc ttttataaga taattcattc tatgttcaaa
     4561 ttgttctcta ttccacgttc ctgtgataga atcaaattta tttttttcac caatattctt
     4621 tattctcccg actagaaata tagtttttgg gtcatccgac acataaactt tacctctaca
     4681 agaaatccat actatttctt tatatttatt aatcaaccta tattctaaat tgtgctcacc
     4741 tttttttcca tgtagtaatt cttgtatatc atctttccat atttgaacat catccggata
     4801 aacaatttta ctccaacaat tgaccaaatc agattctatc tctttactta agttaaattc
     4861 atcaaaaatt gctgaagaaa ttttaaattt attcctattt atatcccaaa aaaatatata
     4921 atcttctgta ctttgagata aaagttcaaa gaaaatttct atatggtcta aacttaaaga
     4981 gttcgattct tctctcatct tttcccccta caatattact attagtgtag tttaatcaac
     5041 aaaatatcct tatttagatt atactatttt actaattttt tgtctcatca aacacatttc
     5101 cccaacttta gttatcttat caaatctctt actcttaact aagttttttg agattcataa
     5161 agtaattttt ctataatcat tactggtgat ttatttttac ctgctaaaac accatgttaa
     5221 atcatttatt aaatcatttt ctcttaaaat tctatttttc gttatataaa gagttatcag
     5281 ttctttattc tataaaactt ttctattact cttgaattta ttttattttt ttcttcgcct
     5341 attctctttg gatttggtat tgcttttcta tagattttgg caaagaagtg tctcttccta
     5401 taatataaga ttttgatatt tcttcatcat ctataaattt tttaatagg
//
LOCUS       DAMJZE010000221         5352 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJZE010000221 DAMJZE010000000
VERSION     DAMJZE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.225"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(204..1388)
                     /locus_tag="PC541_003502"
     CDS             complement(204..1388)
                     /locus_tag="PC541_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2840923.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="PC541_003503"
     CDS             1761..4112
                     /locus_tag="PC541_003503"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2840924.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="PC541_003504"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="PC541_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2840925.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2097 a          598 c          851 g         1806 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttca tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa ccatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcat ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tattgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcat aa
//
LOCUS       DAMJZE010000222         5340 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJZE010000222 DAMJZE010000000
VERSION     DAMJZE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.226"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(214..2493)
                     /locus_tag="PC541_003505"
     CDS             complement(214..2493)
                     /locus_tag="PC541_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2840926.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2764..4218)
                     /locus_tag="PC541_003506"
     CDS             complement(2764..4218)
                     /locus_tag="PC541_003506"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2840927.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4382..5251
                     /locus_tag="PC541_003507"
     CDS             4382..5251
                     /locus_tag="PC541_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2840928.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1821 a          832 c          569 g         2118 t
ORIGIN      
        1 aagtattttg ctataatccc atatactctt acctcaaaaa cattcaaatg taaaaaatac
       61 tacatattaa taaactttgc ccaataattc ctaaaataat atcctaacaa tgatttatgt
      121 ttttataata gtatattttt acataaaaaa gtgaacattg attaacaaag ttcacttttt
      181 tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc tatttaaact
      241 atatctatct aatattaatt tttcatattc ttctattggc attggtctag caaataaaaa
      301 tccttgggcc atatcacaac ctataaacct taaaaactct gcttgctcta cagtttcaac
      361 accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac ttgaaactac
      421 aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta atttcaatac
      481 atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa aatcatccat
      541 agaaatttta aatccaacag atttcaaagt ttgcattatt ttaaataata tttttgtatt
      601 gtcaaatatt gcactttctg tcaattcaat ctcaattaaa tttggttcaa tttcatattc
      661 ttttgttatt ctttcaagct cagaaataaa gcctggattc tcaagatgta ctcttgataa
      721 gtttattgat ataggaaatg tggggaaatt ttcattttcc caacgtttaa aatttttaca
      781 aacttgggta aatacaaaca tatccaattc tgttataaaa ccatttttct caaaaatagg
      841 aataaataca cctggactta ttagaccttt tttgggatgt tgccaacgta caagtgcttc
      901 agcacctaca attttagtag ttgataaatc aaccttaggc tgtaaataaa ctttaaattg
      961 ttcatttttt aaagcatcat gcatttcttg ttcaatatca cgttcttcta tgagagtttc
     1021 ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta ctgtagtttt
     1081 tgcaatcata gctttgtcaa gacaggtaga aatatttgtt tctccctctt caacaaaata
     1141 tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta tcattgacaa
     1201 ttttttacgt ataagttcac atatttctat aattgactct ctattatttt tcttttcaaa
     1261 tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt taaatatatt
     1321 cttaattatc tctgctattt cctttaatac tctatctcct tcttcatacc caaacctatc
     1381 atttactagc ttaaacttat ctatatcaaa ataaattaaa actagattct ttttctcatg
     1441 agaggataaa aattttcccc cttcaagatt aaatttattt gaattgccaa tacctgtaac
     1501 gttatcttta aaagcaaatt cttccaattt ttccttactc tttttatgta taaatagtat
     1561 ataaattgtt aatgccgtaa agattataat caagactaca catgctgata atgtaagttt
     1621 tataatagca tatgatttct cagatatagc agttttagga acttcagaaa aaagatacca
     1681 atcattgatt cctattgggg cataacttaa ataatatcct tttccaaaca tactataact
     1741 taatgaacct gatttaaagt tttttatatt tgacttcata ttgttgatac tagattttga
     1801 tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt tggcgttttt
     1861 actgcttgga tttaaaacaa tatcaccatt acttctagtt atataagaat atccttttcc
     1921 attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg tggcaaatat
     1981 aactccttta acctcattat ctttataaat tggtacagaa tatacattaa tattactatt
     2041 ggtattttta tcatcttcta ttacatcttt taattttcct gttataacca cttctccttg
     2101 cattgatttt aagaaataat ctcttttaga gaagtcttct atataaccat ctgtagtgta
     2161 tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa tagcttgtct
     2221 ttttagtata gaaaggatat tatctacttc aaaagactct tctccttcaa taaatattga
     2281 aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat tgacgctttg
     2341 atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat tcaaccttga
     2401 accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa ttatggacaa
     2461 accataccta ttattttttg catcacttaa cattatgctt cctgcctttc tcgaatctat
     2521 gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa tacttatttt
     2581 tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa attaattaat
     2641 atagatatta gtgtttacat tattgcatta tctattttac aattatttac atataaaaaa
     2701 atgtaatatc aaagtgaagc ttgatattac atttttgttc ttgtattttg tttaattttt
     2761 atgctacatc ttacttaaac actcatttgc aacttcttta tcatcaaaat gatgcttaac
     2821 tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa catcatcctt
     2881 ttttgccata cttatagctt ctgctatagc ttgttctcta tcaacaacaa catggtaatt
     2941 ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg gttcttcagt
     3001 tcttggatta tcagaagtta taatacataa atcagaatat ttttgtccaa ttgctcccat
     3061 caaaggtctc ttttcagtat ctctatctcc accacaccca aacacagaaa ttatttttcc
     3121 ttctgcaaat tgttgagttg tctttagtac attttcaagt gcgtcaggag tatgagcata
     3181 gtctacaatt acgcttatac ctttatcatt tggaactgtt tcaaaccttc ctgatactcc
     3241 attagaaagt ctaaggcctt ctttatatat tggtttaggt atgccaagta catagcaagc
     3301 agctataact gctaaagtat tatatactgt aaacatccct ggaactggta taaatattac
     3361 ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg attttatatc
     3421 tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta catttatacc
     3481 ttttatattt tcatatatct tttttccacc ttcatcatct atgtttatta tatttgccat
     3541 agtagtctta aaaaataatt tttcttttgc ttttctataa tcctctaaat ctttatgaaa
     3601 atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg tttcatcaac
     3661 tctatttaat gccaatgaat gtgatgaaac ttccattgca cagtagtcac atccattatc
     3721 caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg aattggaaac
     3781 aatgtctttt tctccatcaa aaatttttat agttcctata agtccaactt tattcttatt
     3841 taaagttaat atctcattta gtatagtagt tatactagtt tttccattag tacctgtaac
     3901 tcctataacg ttaaattttt gagatggatg attgtaaaaa ttatcagcta cttttgccat
     3961 atcttttctt gtatctttta cctttataaa tgtacatcct tttattgcta catcttcttc
     4021 aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat gaccatctga
     4081 cacaaatcct tttatacata taaataatgt accttttgta acctttcttg aatcatattg
     4141 tacattatta atatctatat ttaattcacc cttgacattg attatatcca acccctgtat
     4201 aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct aaaattacac
     4261 tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat gcttgaaaca
     4321 ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt ctataaaaac
     4381 tatggataat aaaatcgggt acttaaacga taactttaaa atatttaata ttagagataa
     4441 aaaagatata caatttgaat accataatca tgattttaat aagataatta tatttataaa
     4501 tggaaatgta acttattttg ttgatggtat tccctataaa ctaaagcctt gggatatact
     4561 ctttatcagc aataatgaaa tccataaacc agttatagat tcaaatgtct tttatgagag
     4621 aattgcaata tggattagcc cagaatttat gaaaaaaaat agtgatgcta gtagtgactt
     4681 agaaaaatgt tttaaaatag ctacagagaa taaatgcaat agattgcgtt taaatatgca
     4741 tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat ccaatactga
     4801 atttggaaat gaaatattga gaaatacatt atttatacaa cttatgatat tcttaaacag
     4861 aattttccta aaaaatgaaa atatggaatc aacagaagat gttttttatg atgaaactat
     4921 taagaaagta ttaaactata taaattcaaa tatagatgat aagctttcaa tagatagcat
     4981 atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat ctcatactgg
     5041 aacgtctata cacaattata tagttcaaaa aagattaatt ttggcaaagt cactaataac
     5101 caatggtcac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt attctagttt
     5161 tgtaagggca tttaaaaaaa actacggtgt atctcctaaa aattatataa acaataaaaa
     5221 tcctttagat attactcttt tagatgaata gataatttat gtaaatttta ttttaataaa
     5281 acttgaagta ttagtgtatt tatcttacta atacctcaag ttttttaatg ttttaaaata
//
LOCUS       DAMJZE010000223         5325 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJZE010000223 DAMJZE010000000
VERSION     DAMJZE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.227"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            305..1831
                     /locus_tag="PC541_003508"
     CDS             305..1831
                     /locus_tag="PC541_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2840929.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1859..2416
                     /locus_tag="PC541_003509"
     CDS             1859..2416
                     /locus_tag="PC541_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840930.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2938..3975
                     /locus_tag="PC541_003510"
     CDS             2938..3975
                     /locus_tag="PC541_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840931.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            4086..5228
                     /locus_tag="PC541_003511"
     CDS             4086..5228
                     /locus_tag="PC541_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2840932.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2250 a          441 c          942 g         1692 t
ORIGIN      
        1 aaaataatag taaactgatt gcataaaaat gatttttata taaaatttaa gcagaagaat
       61 aaatttctaa cgaaattgga tatttcttct gtaaatacat attcaaaaaa agaattataa
      121 gagtttagtc agcagtcgtg taagtaataa ggactttata aatatataga aatataatga
      181 tattaatata accaagcgat ttttaattta gctttttaat aagcttattt tttatactat
      241 ttataataaa taaactccat gatgggaaaa ctattactat ttaatatgta aaggagtatt
      301 ttaaatgaat gaatcatatt ggtttttaaa ctcaagtcca aaagaatata acaaattagg
      361 tgaaaatata aaaacagatt gtcttatagt aggtggtgga ataacgggat tgacaactgc
      421 atatttatta gcaaaagaag gtaaaaaagt agtgcttgtt gaggctgata aaataggata
      481 tggaacttct ggaaggaata ctggtaaggt aacttgtcag catgatatat tttactctaa
      541 gatagagaaa aaatatggat tagataaagc aaaatcatat tataatgcaa ataatgaagc
      601 tttaaattta gttgaacaaa taatagaaga aaacaatata aaatgtgatt ttaaaaggga
      661 aacttctttt gtatttacag aaaaagagga tactataaag gatattaaag atgaatatcg
      721 tacatgtaaa aaaataggaa taaattgtga gtatcatgaa actatagaaa atattccatt
      781 agatataaag ggggctatta gttttactaa tcaaggtcaa tttaacccaa agaagtatat
      841 tgatggattg gcaaaagcag cagtaaattt agggctaaaa atatatgaaa atacaccagt
      901 agtagacctt gaaaaaggaa aaatatgtag ggttaaaaca agagaggata atataataga
      961 agctgagaat gttataattt catctcatag tccatggtat gatggattga atttttattt
     1021 tgcaaaagaa tatgcagagc gtgcttattt aatggttgca gttttggaaa ataaattagc
     1081 agatggtatg tttataagta tagatgaccc atcaataact tttagacaat ataatgatgg
     1141 aagtgaaaat ttactgattt ttggaggagg agaccataaa gttggtcagg gaggaactga
     1201 gaaagaaata tttgatgatt tagagcatta tggtaaagaa gtctttaagg taaaagattt
     1261 taaggggaaa tggtctgctc aagataatat gagttttgac aatgttcctt atattggtta
     1321 tatcaacaaa agagaagaca atatttatgt agctacaggg ttctctaaat ggggaattac
     1381 taatggaact gctgctggaa taataataaa agatttaata ataaataata attctgatta
     1441 taaagatact tttaatccat ccagactagg tagctacttt tcaaaagatt ttataaagga
     1501 aaatgcaaac gtagcaataa attatgtaag tggaaaatta aaaataggta gtggagatat
     1561 gcctaaaaat aatggagaag gaaagattgt taatattgat ggaaaaagat atggtgtata
     1621 taaggatgat aatggagatt tttatattgt agacactaca tgtactcatt taggatgtga
     1681 acttaatttt aacagtgaag aaaaaacatg ggactgtcca tgtcatggtt ctagatttga
     1741 ctataaggga aatatacttg aaggaccagc tcttaagcca ttaaaacttt atggacatgg
     1801 tgataatgat gttaatccta agttattata aaattatttt taggaaggta aagtgtatat
     1861 gaataaactt ttaaagccaa taactatact attgtatata gtttatattt tatctgccgt
     1921 attttttgta gcaaacaaag aatatggaag tctaggtcta gtagcaatta gtttagttgg
     1981 tactttcatt ttgagtttta taaataagca aactgaaaga ttattgagta aagaattata
     2041 tataatgctt gctttattta taatgttttc atcattgtta ggtacttgtt ttaattttta
     2101 tgatataaat tattatgatg attttttgca tttatggtca ggtataatat cttgtattgt
     2161 ggctttttct ataattaaat acttttatac acaaagagag ataagtaaaa tgagtaaagt
     2221 gtttttagtt atatttttat ttatgtttag tatgggagta gcgagtttat gggaaatagg
     2281 tgaattttta atggatactt ttgttgggac tactacacag gccggagggt tagaagatac
     2341 tatgattgat atggtagatg ctttaatagg cacaattata actattcctt ttatagtaaa
     2401 aaaatctaat atatagtttt aaatttttta tattaattat aatggattta attaatcatg
     2461 ttgagtttca aggtctatat gattaattaa atccattttt gttatttata attattattg
     2521 aatatatttt tacatataag atataaggtg atagatagaa attgttggat tatgtaaata
     2581 attaatatat aataccatta tgggcaataa atacaaatca aatgataata tagtctattt
     2641 atgtaaatgt tatattgtat cctaagtata atagtaagtt ttttgatagt ggtgtagaaa
     2701 gtaaaaatct actagcctaa attgatgtat ggattagttt aggaacagta attgcatatc
     2761 ttaagtttga tgtcatgttg ggctactaga aataggtcat ctttttcact tgaatatctt
     2821 ttctttaaac atggggagta tcatatcaaa aacatatgaa aattaagtga tttatgagaa
     2881 cagtaatatt ttatatgata gtatagaata gtaataaagt attaggagga acattgagtg
     2941 ttaaaaaagt ggtttggtat tgtgaaaaaa agacaaaaaa gtgagtcagt aaaagaagaa
     3001 ggtgaagtaa tattaaaaaa cgaaaaaata ttatctgaag aaaagttgat agatgaagaa
     3061 ggagttgtag ttaatattga taatcaaata ttaactaaag tagtaaatga tgacaatgaa
     3121 atagaagaaa aaataataga agaagatttg ttaattagtg aaaataccat aaaattagat
     3181 gataaagaag caattattaa tgataaaaac atagaattat gtaaaaaaga agttcaagtt
     3241 gaaagtgaaa aaataggttt aaataagttt gaaggactgg accaaaatga aaattataat
     3301 ctagaaaaaa atgttattga agaaaaaaaa gtaagtgaat gtttgacaga agaagattta
     3361 gagtacataa aagaaattaa aataaaaaga ggaaaaagta taaaagctat aaacttgtat
     3421 actaaagaag agtgggtttt tgacactcat atacagtgta gtaaaaaact caaagttcca
     3481 ttagggtaca taagagaaaa tttaaaatat ggatatatgg attactttgg agatgcaata
     3541 aattatttaa gtgaagtatt aaatatagat gaatactgca aaagtgaatg gagctatctc
     3601 gataatagca aatctccatc tgagatattt aatattctaa acaataaaat atttagtata
     3661 aggctttcta atgaaaaaag aaatgaaatt ttgacaaatg ataaaattga agcattaaaa
     3721 atgaattata gatttgaatg tattgatgaa gaatatgatg aatattttaa aaaatataag
     3781 tctataatca aaagaggtgg aaagaaaaaa gttgaattag taaataaaaa aggtgacatt
     3841 ttagaaatat ttaagtcttt agaagaatgt gccatttatt tgcaaaagga aaagaatgaa
     3901 gttatacaaa tgttaaaata tggagataca aaagtaggaa gaaactttat aaggtatagt
     3961 ttgagaagta tttaatttca ttttaaattc ataagagttt catttctgat tcacacattt
     4021 aatattattt taatgtaaaa taataaaggt gaagtattgc acaattaaaa ttaaggaggg
     4081 tattaatgtc tgaagaaaaa aatccaaaag aaaatacatt agatgaatct aaaaattcag
     4141 ataatgtaaa ttctaaaaaa gatgatgtgt cagatgaaaa aaatactgat aacaaagtta
     4201 aaaaaaggga aataaagcta aacaacaaaa cacttaaagc tattgtagca attgttgcag
     4261 taggagctat atgtttaggt ggaggctatg tttttggcaa agaagctgga agaaaattgc
     4321 ctgctacttc aaaacactat aatagcagtg aggtagtagc tactattggg gataaaaaaa
     4381 taacaggtga aatgttaaga aagagaatgg agccattatt ttatttaaat gcaaaaacta
     4441 ctatgactga tgacgaaata cgttcatatg aatcaaatat gattgaaact atgattacat
     4501 ctgatatatt atcctctgaa gcagcaaaag agaaaataag tataactaat gaccaactag
     4561 atgctcagta taataattta atgagtagta tagaatcagc aatgggaatg actaaagaag
     4621 agtttttaaa acagtttgat ttgactgaaa aatatataaa agatgatatg aaaaaagagc
     4681 tagtagcatc aaaatattta aatgaaaaat caaaagttac tgataaagaa gcagagaact
     4741 attacaataa aaacaagagc aattatttac aagttagagc ttcacatatt ttaataaaaa
     4801 ctgttgatga taaaggtaag caagttagta gtagcaaaaa agctgaactt aagaaagaag
     4861 ctgaagaaat acttaaaaaa gcccaagctg gggaagattt tgctactcta gctaaaaaat
     4921 actctgaaga tagtagtgca gaatcaggtg gagatttagg tttctttggt aaaggtcaga
     4981 tggtggaatc ttttgaaaaa gcagcctttg cattgaagaa gggagaggta tctaacaaac
     5041 ttgtagaaag tgattatgga tatcatataa ttaagaaaac tgatgaaaaa tatcagccat
     5101 ttgaagaggt aaaatcagat ttagtaagca gtttaacatc agaaaaacaa aacctactta
     5161 tacaaaatct aaaagaaaaa tataatgtta aaattacaga taaatatcaa atgaaagcta
     5221 aagaatagat aaagtcaatt tattaaaaaa aagaaaaatt aactgtatgg ataaaagcta
     5281 tttttactct caatatagag agtataaaat agcttttatt atttt
//
LOCUS       DAMJZE010000224         5289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJZE010000224 DAMJZE010000000
VERSION     DAMJZE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.228"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            99..935
                     /locus_tag="PC541_003512"
     CDS             99..935
                     /locus_tag="PC541_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2840933.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1288..2865
                     /locus_tag="PC541_003513"
     CDS             1288..2865
                     /locus_tag="PC541_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2840934.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2858..4042
                     /locus_tag="PC541_003514"
     CDS             2858..4042
                     /locus_tag="PC541_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2840935.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4060..4659
                     /locus_tag="PC541_003515"
     CDS             4060..4659
                     /locus_tag="PC541_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2840936.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4683..5153
                     /locus_tag="PC541_003516"
     CDS             4683..5153
                     /locus_tag="PC541_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2840937.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1976 a          559 c         1017 g         1737 t
ORIGIN      
        1 aaaatatata cgaaagtaaa attttaaata ttgacattaa attaaatata ctatactatg
       61 taagtgtgat gagagaattt ttttagaggg gatttatagt gaatataaaa aagatattta
      121 ataacaatgt agtagtaagc tccctagaag atggcacaga aataattgtc actggtgctg
      181 gagtaggatt taagaaaaaa gtaggcgatt tgatagatga aaatttgatt tctaagaaat
      241 attttgttca agacgatcaa agggacaaat acaatcaaat acttaataag acctctattg
      301 aatactttaa aatttcagaa gaaattatag aaaaggctaa tgaagtttta aatactcaag
      361 tgaatgattc aataatatta gccttaacta gtcacataga atttgctgtc caaagagaaa
      421 aacaaggtat aaaattacca aacttaatat tgaatgagac aaaacaactt tatagggaag
      481 aatttgaatt tggattatgg gcaattgatg aaattgaaaa aaggatagga ataaaattgc
      541 ctgaagatga agcaggatat attgctattc acataatcaa tggtttggaa aattctcaaa
      601 aagatgaagg tattaatata ctagagtttt caaaacaagt tattcagata attgaagaag
      661 tacatggatt taaattggat gtaaattcat taaattatac tagacttata actcacttaa
      721 agttttttgt tcaaaggatt ctccgaaaag aaacttacaa tgatagtgat attgaagata
      781 tgtataagtt agtgaataaa aattacaata aacctaaagt gtgtactgaa gaaattgcag
      841 cattggtatt agacagattt gaatataaga ttagtaaaga agaagaatta tatttaatga
      901 ttcacataaa taaaataaca aacagtggtt gttaggattg taactgataa agcaggcaaa
      961 acctaagtca ttgagcttaa ctattagatt aagttttttg atttaggttt ttttattaga
     1021 tacaatcaaa atacagacag tgacttttag gattgtaact ggttaagcag gcaaaaccta
     1081 aatcattgaa tttaactatt aggttaagtt ttttgattta ggttttttta ttagatacaa
     1141 tcaaatacag acagtggttg ttaggattgt aactggttga gcaggcaaaa cctaaatcat
     1201 taaacttaat tattaaatta agttttttga tttaggtttt tctataaatt tgattatgtt
     1261 tttagataaa aataggaggg gaaaattatg aaaaaaaata agttaggttt atggaacttc
     1321 ttccaaatgt taggaaaaac gtttatgttc ccaatagcac tacttagtgt ttctgggatg
     1381 atgctaggac ttggggcagg ttttacagac cctaagatga ttgaaatggt tccattttta
     1441 gggaatgaat atgttaactt agttttaaac ttcatgttaa caataggatt atttgcattt
     1501 aacaacttgg gagcattatt tgcaatggca attccactag gattattaaa aaaagaaaaa
     1561 gagtttggtg ctttttcagg attagtagga tttatagcaa tgcatatagg tacaaatttt
     1621 tatttaacaa gagctgattt gttagtacca gcagaacaga tgtcaactaa tggtcaagct
     1681 atgattatgg gtatacaaac atataataca agtgttttag gtggtattgt agcaggatta
     1741 attgtttatg caatatatga taaagttagt aatttaaaga taccagaatc attaggattc
     1801 tacagtggac caagattagt tccaattgtt agtttaatag ttatgagtat agttggatta
     1861 ttaataccta ttgtttggcc accatttttc aatgcattcc aaagtttagg aagatggatt
     1921 tcttcagcag gaccattagg atatttttca tatgcagtgg cagaaagagt tacaattcca
     1981 tttggactaa atcatcttgt aacatctgta ttccgtttta caccaatagg tggaactgct
     2041 gttattgatg gagaaacgta ctttggtaca ttgaacatgt ttatggcata tgtagaaaat
     2101 aatcagatta tacctcttga tttagctggt aaaatggaac aaggtaaatt gatgattcaa
     2161 tatggattag ctggtgctgc attggctatc taccgtacag caaaaccaga aaatagaaag
     2221 gcacttaaag gacttttaat atcaggagtg ttgacagtta ttataggtgg tataagtgaa
     2281 ccaatagagt ttctattctt attcatatca ccagcattat ttgctttcca tacatttatg
     2341 aatggtctag caaacatggt acttccatac ttaggcgtgt tgatgggatt cacaggagat
     2401 ttaattgcat ttataagttt tggtgtgtta agaggaacag ctacaggatg gccaatagca
     2461 gtattagtag cagttttata ttttgcaata tactattttg tatttaagtg ggcaattatt
     2521 aagtttaata taaagacccc aggacgtgag gataaagaaa taaaagtatc tgaaggaaat
     2581 ggttcaaatt tcaaaaattt gtctacatat aaaggacaac aaatgatatc tgctttaggt
     2641 ggtcaatcta atatattatc actagataat tgtgtaacta gattacgttt aaaattacaa
     2701 gatgtatcat taataaatga ggatgcaatt aaagaggctg gtggtatagc agttgttaaa
     2761 ttggatgagc atacaataca agtaattata ggaacccaag tgtattcttt aagaaagcag
     2821 atggataaag ctatggaagg tcatttggag gcatgtaatg attaattttg ataaaaaagt
     2881 agaccgattg ggtacatatt gtacccagtg ggactatgta gaagatagat ttggtaagaa
     2941 ggatttgctt ccttttacaa tttcagatat ggatttagag tttccatttg aaataacaga
     3001 ggtgttgcag gaaaggttaa atcatagagt tttgggatac agtagatgga aacatgatga
     3061 ttttagaaat tcagttacga attggtatat taaaagattt aattgtagcg ttgatagtaa
     3121 ctggatatat tatagtccaa gtgtaatgta ttcaattagc aaacttattg agatgttaag
     3181 taatgaagga gatggaatcc ttataaatac tcctgcatac aatgcttttt atgatgtgat
     3241 aataaataac aatcgtaata ttataaagtc acatcttgta aataggaatg gattctattc
     3301 aattgatttt aatgattttg agaaaaagtg taaaaaatct aagatattta ttttatgtaa
     3361 tcctcacaat ccaactggta gagtatggag tgagttagaa cttgttaaga tgataggaat
     3421 ttgtaagaat aatgatgtca agataatatc agatgatata cacatggata ttgtatatga
     3481 caatagaatg acacctatct taaatgttgc aaaggactat ttagattctg tattcatttg
     3541 tacatcagcg tctaaatctt tcaatatacc agcattgact gggtcatatg tgattatacc
     3601 aaatgaaggt attagaaata aatttgaaaa tatcactaga taccgtgatt ttgtaaattc
     3661 gccagcaatt ctagcaatat tagccacaat ggtctgttat gatgactgcg aatattggtt
     3721 agatgaacta ttaaaatacc ttaaagagaa tcttaaatat acgattgatt atataaaaga
     3781 taatttagaa ccattgaaat tggtcatgcc agaaggctgt tactttgctt ggatagattt
     3841 ttccgaactt aaaataagtg gtgagaaatt ccaaaaatta ttgatggata ttggagaagt
     3901 tgctattatg tcggggtctg tatatggtga agaaggtgag ttttatttaa ggttaaatgt
     3961 ggcatgttca agagaaaaac ttgaagatgg tttaaatagg attaaaaaaa ctatggatta
     4021 tattgaaaaa agaattttat agtaggaagg gagatagtta tgggatattt aaaaacaatg
     4081 gttgatgtta tgactactga aatgaatgcc ataagatgtt taatagatga agcaggaata
     4141 gaatatgaaa gtatagttag tgaaattgca aattgtgaag gcaaagtaat ttttatgggt
     4201 gtaggaaaat ctgcacatat aggtaagaaa ttggcagcta catttgctag tactggtaca
     4261 cctagctttt ttgtacatgc aacagaggca gtgcatggag atttaggaat gattgaaagt
     4321 aaggatatta caattttaat atcaaatagt ggaaattcta tggaagtggt aaactgtata
     4381 aaatatatta aagcaatagg ttctaagaca attgcattta cttcaaacag aaattcagta
     4441 ctggcaaaag agtgtgatta tgctttaata tatcctgcaa aggatgaagc agaccattta
     4501 aatctagcac caacaacatc atctacaatt actttggttt taggagattc tattgcatgt
     4561 gcattatcta agagtagtaa ttttggttct tcagattttt ataagtacca tccaggagga
     4621 agtttaggtg agaagttaaa aacagctaac aatggataaa agcaaaaagg agagtagttt
     4681 ttatgttcaa aatgtttaaa aaaaaatcaa taaaatcacc catatcagga aaagtaatag
     4741 agttatctaa aatacaagat ggtgtatttt ctcaaaaaat tatgggagag ggtgtagcta
     4801 tagattctac aggagacatt gtatatgctc caatcaatgg aaagatagta gtagtagcag
     4861 agactaaaca tgcatttgta atagaattag aaaatggaat ggaacttctt attcatgttg
     4921 gtttagatac tgtaaattta aaaggcgaag gttttgaggc attggtgagt atgggagaac
     4981 aagtgaagga aggaacacca atgttgaaaa ttaaccgtcc tttgattgaa tcaaagggta
     5041 tatcacttat tactccagtt acaataacta atcattcaga gtacaatatg aatacatgta
     5101 atgtaggcaa tagtgttgaa ggtgggaaag atactgtaat tgagtttaaa taaagaacta
     5161 aaatacaagg ttaatattta taaataccta taaacttatg ttttataaga ttgattgtat
     5221 agatttgttg tataaaaaag aactctttaa ttataaatta aggagttctt ttttattttg
     5281 taaatattg
//
LOCUS       DAMJZE010000225         5195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJZE010000225 DAMJZE010000000
VERSION     DAMJZE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.229"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            103..1566
                     /locus_tag="PC541_003517"
     CDS             103..1566
                     /locus_tag="PC541_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2840938.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1622..2758
                     /locus_tag="PC541_003518"
     CDS             1622..2758
                     /locus_tag="PC541_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2840939.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            2994..4109
                     /locus_tag="PC541_003519"
     CDS             2994..4109
                     /locus_tag="PC541_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2840940.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4261..5130
                     /locus_tag="PC541_003520"
     CDS             4261..5130
                     /locus_tag="PC541_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2840941.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         1993 a          539 c          985 g         1678 t
ORIGIN      
        1 ggtagatttt ttagatatat taatataaag attatataaa atattgaaat atagctgttt
       61 aaattattgt ctataaatat aaaatgaatg gtggtgagtt aagtgaccga tttagttaac
      121 accattaatg gaattgtttg gtcacccgta ttagttgcaa tgtgtttgtg tataggatta
      181 tatttttcat tgaaattaaa attttttcaa ataagagatg taaaagaaat gtttactctc
      241 ttgcttgagg gagatagttc tcatgttggt atatcatcat ttcaaggttt tgctacatct
      301 atggctggaa gaatagggac tggaaatata gctggtgtag ctgtagcaat agcaatggga
      361 ggtccaggag cattagtatg gatgtgtata atggctacat taggttcagc aactgcattt
      421 gtagaatcta ctttagctca aatatataaa gatgagcatg atgggcagta tagaggagga
      481 cctccatatt attttgaaaa aggtcttggg tggaaaaagt gttcagtttt attttcaata
      541 gttgcaataa taagttatac attttttatg cctggtacac aatcaaacac tttatatcta
      601 gcaatgaatc aagcatttgg attttcaaaa atgacaatag cagtagcatc ttctattgca
      661 ttagcgataa taatttttgg aggaattaaa aggattggtt tatttgctga aaaagtagta
      721 cctataatgg gtggagcata ccttattatg acattagtaa taattataac aaatattagt
      781 ttagttccag aggctattgt aacaatgttt aaatctgcat ttggatttgg ttcagttttt
      841 ggaggaatgc taggttcagc aatatcttgg ggagtaaaaa gaggtatata ctcaaatgaa
      901 gcaggagaag gttctgggac ttatggtgcg gcagcagcag aagtaagtca tccagccaaa
      961 caaggattag ttcaagcatt ttctgtttat gtagacacat tattaatatg tatggctaca
     1021 ggtattatga ttgtaataac aggaatgtat aatgttacag gtgtttgcac agcactacca
     1081 ggtgtagaac cgggaccaca atatgtacag agtgcaataa atacattaca tccacaatta
     1141 ggtgatattt ttatagcaat agttttatta ttatttacat ttacaacttt attagccttc
     1201 tcatatatga tggagactaa tgtatcttac ttagtaacta gaattaaagc agaaaaacat
     1261 gaaaagtaca taattaatgt ctgtagattt atgcttatag catgtgcagc aatgtgttgt
     1321 tttatagaac cacttacaag ctgggcatta ggtgatatat gtataggtgt attaacatat
     1381 ataaatttaa ttgccatctt tacactaaaa aaaccagcta taaaggcata ttatgattat
     1441 ataagacaga agaagatggg aataaaaagg agtgatagaa cttttaaccc tatagagtta
     1501 gatataagaa atgctgagtt ttgggaaaat agatacttaa aagaggataa gcatgagtta
     1561 aattaaagtt ataataaaat ataaaaatta ctaaataaat ttagtgggag gtaagattgg
     1621 tatgaaagaa agaataatac aagtggctga ttcaaagaaa gaaaaaattc tagggctatg
     1681 tcaatcttta tatgatgaac cagaaattgc tttgcaagaa tataaatctg ctaaaaaaat
     1741 atcagaattt ctaagagaag agggttttga tgttgaggaa aacttggcag gtatggctac
     1801 agcttttaaa gcaaccaaga aaaatggaga tggtcctaaa atagcattta tagcagaata
     1861 tgatgctctt ccaggcaatg gacatgcatg tggacatcat ttaattgcat ctatgggagt
     1921 tggagctgga atagctcttt caagtatact ggatacttac aagggagaag taagtataat
     1981 tggaacacct gctgaagaaa ctggagatgg aaagccttac ttgatagaac atggtgtatt
     2041 tgatggctat gatgcagcaa tgatgataca tccaaattca aaaacttgtg taacacctga
     2101 aattatagca ataggaggtc tagatttcat atttactgga aaagcatctc atgcaggagc
     2161 taagccatat aacggaataa atgctttaga tgcagtagta ctactatata ataatataaa
     2221 tgcactaaga caacaattag tagatggaac gagaatacat ggtattatat tagaagcagg
     2281 aactgctgca aatgtaatac ctgatatggg aaaagtaaga ttagagataa gggcaaaaga
     2341 acaaaattac tttgatgaag tagtagaaaa agtgaaaaat tgtgctagag gtgctgcaat
     2401 agccactgga tgtgaacttg aattttatca ctttgaacca acttgccaag gattaaatga
     2461 aaataaagtt ttggtagaca tatttacaaa gattatggag gaatttggaa tatatgaaga
     2521 tgaacaagta atgttaggtt caacagatat gggaaattta agtcaaataa tgccatgtat
     2581 acatcctttg atgaagtttt ctgaaaatgg agaagaacta catacaaaag aatttttgga
     2641 ggcttctata aattcatatg caaaagatag agtaattgat ggtataaaaa tattagcttt
     2701 aacaggattt aatttatttg aaaatccaga attattgaaa aaaatgaagg aagagtagaa
     2761 cttagactat ttttaattat actctttaaa ataagaagag tgtctcaaaa tgaacttttt
     2821 agtttatgag acactctttt ttgtatgaaa ttaaatatat ttttattatt ttaaacatga
     2881 aaaagaggct tatctgtttt tatatgacct tttttaactt gtagcacaaa atatcatgaa
     2941 tgattacaat tcgaatgata aaatcgatta taagatatat aggaggggta catatggaaa
     3001 ctttagaagt aagaaatatt gaattgagtg gtacaaatta tgagatagga tacagactag
     3061 gagaattggt tgctaatatg ccagagataa tagaaggtca gattaataaa tctaatctgg
     3121 taagcaaaaa agaagaaaaa gagatgatag agttgtttga taaatattgt ccaggtctta
     3181 atgaagaatt gcaaggtttt gctgatgcaa ttcaagtgaa ttgtaatcaa attttatatt
     3241 atactatgac ttatcttaaa ccaggttgta gtcaagttgc attagcacca gaattaactg
     3301 aaaatgggca tgtactattt gcacgtaatt tcgatttctc acacaatatg gaggattttg
     3361 ttttatgtaa aactaaggta aatggaaaat atgcacatat aggtactacc attatgcaat
     3421 ttggaagagg tgaaggtatg aatgaatgtg gcttaggtgt aagtcagtca tcatgtggta
     3481 ttcctgtagg caattcagat ggtttaagaa aaccagcaat tgtaggttta cagttttggg
     3541 ctgttattag atatttgtta gaaaattgca aagatgtaga tgaagcatta gaatatttaa
     3601 aagatatgcc aattgcatac aatattaatt tattgcttgc agataagtct ggaaacattg
     3661 cacttgtgga gactttagat gggaaaaaag aagtaaatat aatcaacagt ttagaaagta
     3721 aaagagaata ttttttacac tctactaatc atacacatat tgataaactt cataaattag
     3781 atccacaatc aatgaaaaat tctattcata gatataaatt aattaaagaa tatataaata
     3841 agtctaaaaa aattggagag aaagagttaa tgaatttatt gtcttcaaaa tatccaaatg
     3901 gcttatcttg taattactat aatgattttt ttggaacttt aaaaagtata gttatggatt
     3961 taaatatagg aaagtttaat attttatggg gtggactaga gaataaatgg gagagctact
     4021 atttaaaaaa tgacattaaa tctataacac agaggataaa cataaatatt gaaaaagcac
     4081 catctgactt ttttgatttc attgaataaa aaatgtattt aattattcta aataattagg
     4141 acatataaat caactaaaag tttttattta tcgtattgaa ttagttataa tattttagta
     4201 tagtaaaaaa tagtgctatt ttacatattt aaagagttta agtagaagag aggtgccata
     4261 ttggattact taaagtattt tgaaaaagct gtaatgtata ttgagaataa tttacatgaa
     4321 aatataactg taaatgatgt ggcaaaagaa actggatatt cttattatca tttaacacgc
     4381 ttatttaagt ctatgtttgg tgaaagtgta ggaagttata taaaaaaaag acgattagta
     4441 agtagtacaa aagaactttt atatagtgat aaaaaagtga tagatatagc tattggtagt
     4501 ggatttgaat cttcagaagc atttagcaga gcatttaaat ctatatacaa ggttagccct
     4561 atagaatata gaaaaaatag aattgatgta tttgttggta aaaagaaaaa attagagtta
     4621 gattttatga gacatcttgt tggaaatata acgattaaac cagttataaa agaaatagaa
     4681 aaaataaagg taataggtat aaaggataaa gtaattttag aaaataattc attacctgac
     4741 ttgtgggaga aattcagaaa agtacatcat attgtcccaa atactttgcc atcaaggaga
     4801 gtttttggaa tctgtgaagc aatctctgaa atacacctag ttagtgaaag tatggagttt
     4861 aatgagataa taggacttga agttaatagt tatgatgtga ttccaaattc atttgtttca
     4921 aaaactatta aggaaggaaa gtatgctata tttactcata ctggtagctt agataattta
     4981 gacaaaactt atgaatatat atggggtact tggtttttaa gttcaaaaga agaacttgat
     5041 ataagagatg attttgaagt atatgatgaa cgatttttag gtcctagtaa tgtgaactca
     5101 caaattgata tttatatacc tataaaataa tctataagaa aactaaaaga taatttgatt
     5161 aagaaataga tgttcgtaaa agagcatcta ttttt
//
LOCUS       DAMJZE010000226         5082 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJZE010000226 DAMJZE010000000
VERSION     DAMJZE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.230"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            276..1613
                     /locus_tag="PC541_003521"
     CDS             276..1613
                     /locus_tag="PC541_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2840942.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1949..2518)
                     /locus_tag="PC541_003522"
     CDS             complement(1949..2518)
                     /locus_tag="PC541_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2840943.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2888..3493
                     /locus_tag="PC541_003523"
     CDS             2888..3493
                     /locus_tag="PC541_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HDF2840944.1"
                     /translation="MTNWNLWRTLNMEYIINPCSGIKIKITRGQKIEIVDIEGGQVVD
                     FFAEMKDDSTEFISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLS
                     KANDKIILEALENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(4172..5074)
                     /locus_tag="PC541_003524"
     CDS             complement(4172..5074)
                     /locus_tag="PC541_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2840945.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         1834 a          826 c          586 g         1836 t
ORIGIN      
        1 aaacttaatt ttttaaagct cagtttctag aatttcaatc tttcaaaatt caattttttg
       61 aagcttagtt tttagagttt ttttcaaatt taattttttg aaacttaatt tttaaaattt
      121 caatctttta aaactcaatt ttttgaagct taatttctag aattttattt ttcaaaatca
      181 attctttgaa atctaatttc cagaatttta atttttaaga tttaattttt taaaacttag
      241 tttccagaat cttaattaat agaaaggtat attaaatggg cacttattct ttaatctcta
      301 caagcataaa ttttattcta atattaatca atattttcat agcctttttt ctttataagt
      361 tgacttccta ttttatagac tttaggaatc attggatttt caaaatcata ttgattttgg
      421 gctactctat tattagtcaa atgataatat ggctttccga ccccataaat attttgttta
      481 cactattcgg atattttata ctacttataa tatgtagcaa aagcaaatta attcctataa
      541 tatcagttgt catgatactt tatcctatta taattgggct taattttctt tttataaaca
      601 accctatata tgaggaactt atacactcaa aagagtatat tttaatactt tcaatagtcc
      661 agataatttc tgctctaatt aaattattat tttggttttt aatacatatt ttcctaaaga
      721 atagattggt caatataaaa caatacttat caaataaaat atggattttt attgatatgg
      781 tatgtatatc ttcattttta tcaatattaa ttgctattat tctgccacct actaatttaa
      841 tccctatggg tagtaataag aatattatct taggcaactt accttatggc acttatctaa
      901 tcatcttgtc agcaattctg gcaaatatcg gtgtgatact tcttttacag ccattgattg
      961 aaaatgtaaa gatgaagata gaaaaacaag caaatcacct aaaagaaaaa tactataact
     1021 tactagaaaa tcaacaaatt caaatacgca aaatgcgaca tgatatgaat aatcactttc
     1081 aaatgattga atcgtattta gaaattgaag atattgctgg agctaaaaag tattttaatc
     1141 agttaaaaat aggtataggt actcttagtg gaaaacaatt ttgcaaaaac caagctctta
     1201 actcaatttt aaatcttcgt catacaagtt tagaagaaaa tggtgttgat gtgcacttta
     1261 atatagatat agacaatcac ttgggtatag aacctataga tttatgtact attttttcaa
     1321 attctttaga taacgctata gaagcatctt tgaaaattcc tgatttgtca aatagaaaag
     1381 tggtattaaa ggcacgttgt gagaaggagt attttagcta tttattgaca aacaacaagg
     1441 taaatcctat caatagaaaa caagaactat ttatttcaga taaaaaagat agtaattctc
     1501 acggatatgg tattgagaat attagagaaa ttgttaataa gtacaatgga aaattggata
     1561 tttcttatac tgagagtgaa ttttctttat ttctttatat tagattattt tagtctaaaa
     1621 ttagattatc ttatataata caaattagat tgtttgatac tatgtaaaat tataataact
     1681 tataattaaa tatttacaca tatattcatt aacttaaaat ttaatcaaat gtgatatttt
     1741 atttacctta gtaaaaatgt gtgtgtctca aaataaactt tttagttgat gaggcactct
     1801 tttttacatg aaatcaaata tatttttatc ctttcaacaa tgaaaaatgg atttatctct
     1861 ccattttgaa acaagccctt tttctattaa ttactttctt aaaaaatata atacgatata
     1921 taagataaat atctaattgt acctatttct aattatatct attagaatac aacacctttg
     1981 ccttttcagt aaactcccta acaaaatcta gtttactatc tgtataagca tcacgattat
     2041 gctcatactt cttccaaagg tccaacttaa gtttttcata ctcctttgca acatctgggt
     2101 ggtcaattaa gtaatctcta aaaaacaact catcattatc acctacatac cttatatgca
     2161 aatgaaaaac tcgttcttca aacccctcta ctgtataccc tttattaagt gaaagcctac
     2221 tactgctctc agacatacaa atatatccat tcgactcaat caaatcttta atatctttca
     2281 tattataatc aggaattatc tcaatcagta tgtcaacaat gggcttggca taaatcgtac
     2341 tcacagaagt acttccaata tgacttattc taacaatttt ttgcattggc aatattccag
     2401 ttagtctata ttgttcttct ttaaaccaat ttttccaaca ttctttgtga gtagtcaaaa
     2461 atataggaaa caagttccat aactcctcta aagtcatatc ttccaacttc tttttcatta
     2521 gttacctcct tttaaatttg tactttatat tttttctttt tcaaatttac ttagttaata
     2581 tttaacttta ttttttatca aatcattttt ctaattatgt aaatttttct aaatttagtt
     2641 tttaatgtat tataacagat ttttaccctt cttacacttt tgtatctatt taaatattag
     2701 aacatttttt cttacttcta aatacagtaa gattgcttaa ataccaaata ggagcaaaaa
     2761 aactctgtaa tctaaaaaaa actacaacat ctttatgaga attaaaaaat gatagactat
     2821 gttatcaaca ttaagaaaca gatagaaaag aaacagagta tgtgctaaaa tgggaacata
     2881 gaacagaatg acaaattgga atttatggag gacattaaat atggagtata taatcaatcc
     2941 ttgtagtggt ataaaaatta aaataactag agggcagaaa attgaaattg tggatattga
     3001 aggaggacag gtcgttgatt tttttgcaga aatgaaagat gattcaactg aatttatatc
     3061 tccaggtgtt acaattgatt gtaatgagtc attaagatta caagttggga atttaatata
     3121 ttctaataag tatagaccta tgtttaaaat tttatatgat gatgtaaggg aacatgactt
     3181 gctacatcca tgttgtagac ctgagatgta cgatttcttt tatcacaatg gagaaaatca
     3241 tccaaactgt tttgataata ttaatcgcac acttggaact tcccatcaaa ttattcaccc
     3301 cattaatttg tttatgtaca caaaagtcaa tacagatggg agtattgaag tatgtgaacc
     3361 tttatcaaaa gccaatgaca aaattattct tgaagcattg gaaaacatga ctttagggat
     3421 tgctgcatgc agtgtttcag aaagttcttg taatagcaga aaatgtactt ctattaagat
     3481 tttggttgat tgacaaattt catttatcga aaaaatatca aaattaaaaa atgacgtcaa
     3541 aggcgcaaaa gggtaaccac ttgcgctctc aattcattat ggtttgttac gtatttatgg
     3601 gtaaaaatcc cttgattcta tgatgttttt cagacacaat tacaaatgag taaggatttt
     3661 atactcatac tctaaaaaat agcattttat tgcgtaacag aacgaaaaaa cagtgtgtac
     3721 ctttttcttg caatacacac tgtattcata gccactctag ctgttaactg ttaattatct
     3781 cataataccc ttactggtat tcatctaatc ttaacccctt ttaattattt tcatttataa
     3841 ataaattcta ttcttcccaa aatatcgtaa ttaaaatata ttaattaaaa tatcccaata
     3901 agaatatctg aaaaatcttc tacctatttt ttcaaacctt ttattcccat tcctcataca
     3961 atagtgcaac ttttagcaca gtataaatat accattagtc catataatct cattaacaaa
     4021 tatatacaat tattttcttc aaactctttt atatctaaga tatcacattt tcattttctt
     4081 atattaattc cttaactctc tttcaaatta ccaatttttt aatcaaatct tcattaaata
     4141 ttctatcatt attaaacatt catcattatc cctaaaaaat atgtgccgta tgttctttac
     4201 acaaattttc catcttatca tacacagcac cactatctct ctcatctgtc atagtcacaa
     4261 ttacatcact tggtctaaga attgtcttac ctctaggaat aatttcttct ccacctcttt
     4321 gaattgctac caaaagacaa ttttcaggcc actgtacatc ctgaattgcc ttgttcgcaa
     4381 caggagagcc aaacataaca gagaactggt ctagtatctt ttcgcccctc tctttatgta
     4441 caggttctcc attcttttca agtaacctct caagaagact ttcataaata ggtttagact
     4501 tcataagtgt agccacaata tatgccacaa ttgaaattac tgatagtgaa agcatttgac
     4561 taacagaacc tgtcatttca aaaattaata tgataccagt caaaggggca cgcacaatag
     4621 ctgtaaaata tcctgccata gccaaaagca caaagttatt tacataatcg ggattcattc
     4681 caaaaacttt aacacctacc ataccaaata ttccacctat aaatgctcct aaaacaagca
     4741 atggaaaaaa tattccccct ggtgcacctg acccaaaact aacagccgaa aatataaaac
     4801 gtataacaaa aatcaaaaca accattccaa aaatatattt atgacttgta agttcttcaa
     4861 ctaaattgtg tccactcccc agaacatcag gcataacaag cccaagaata ccagccatta
     4921 taaatggaat caacagtttt ccaaacgtac caattttagg aatcttcttg tataaagact
     4981 gcacatatag agtaaaccag ttgtaaaatg cccccataac tccaagaata acacctaaaa
     5041 tcactattaa tccataataa tcttgtggca acacatttcc aa
//
LOCUS       DAMJZE010000227         5063 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJZE010000227 DAMJZE010000000
VERSION     DAMJZE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.231"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(32..232)
                     /locus_tag="PC541_003525"
     CDS             complement(32..232)
                     /locus_tag="PC541_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2840946.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(457..633)
                     /locus_tag="PC541_003526"
     CDS             complement(457..633)
                     /locus_tag="PC541_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840947.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(687..776)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(956..1156)
                     /locus_tag="PC541_003527"
     CDS             complement(956..1156)
                     /locus_tag="PC541_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2840948.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1158..1508)
                     /locus_tag="PC541_003528"
     CDS             complement(1158..1508)
                     /locus_tag="PC541_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2840949.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1670..1858
                     /locus_tag="PC541_003529"
     CDS             1670..1858
                     /locus_tag="PC541_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2840950.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2335..2505)
                     /locus_tag="PC541_003530"
     CDS             complement(2335..2505)
                     /locus_tag="PC541_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840951.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(2894..3382)
                     /locus_tag="PC541_003531"
     CDS             complement(2894..3382)
                     /locus_tag="PC541_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2840952.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4186..4560
                     /locus_tag="PC541_003532"
     CDS             4186..4560
                     /locus_tag="PC541_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2840953.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4665..5039
                     /locus_tag="PC541_003533"
                     /pseudo
     CDS             4665..5039
                     /locus_tag="PC541_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2049 a          620 c          580 g         1814 t
ORIGIN      
        1 cttaaaagag tctttccgaa aaatctatat attataatat agttatattt tctgcttgag
       61 gacctctagc gcctttaact atatcaaagc ttactttttg accttcttct aatgatttaa
      121 atcctgaagt ttgtatagct gagaaatgag caaatacgtc atctccactt tccattgata
      181 taaatccaaa tcctttttca ttattaaacc attttactat tccattattc attaaaaata
      241 ctcctattaa cctatatcaa gatatacttg atttacttgt gtattaccac aaatattatt
      301 gtatcataga ttaacataaa tgtctattta attataattt ttagttaaca tttttattta
      361 ttcttaatat gaaaataaat ttaaacattt tatattacca atatattaat gtaaatgtag
      421 ataaaactaa aaagggtttt ctagaaaaaa atctgtctaa ttatacttac ttaccaatac
      481 catcacttga tgcaagcaag attccacaac tttttctccg tactctaata taaaatcatc
      541 taactgtgta tctataaaat cacaataatc gtatgaatta ctaatataag cagttaacat
      601 atgtgtacat ttatccattt tatctctaaa cataatgtat ttatccccct aaaaaacttt
      661 catgaaaaac aaaaagaaaa tatctttttg gcaactggct gacataactt attttataag
      721 cgttagtccc tatagctttg cgtcactaaa tttctctagt tttgccgatt tagttttatt
      781 ctacagatta ataatacaat agaattagtt atcattcaac atatctagtt gaataattaa
      841 taaatttata tatgaacttt aaattatttg aaaaaataaa aagcccctct gtcaggagtg
      901 acttaaaaat tgaatttaag tttgtattta aaacacattt ttaataatgc aaaaactaat
      961 caaaaataaa caattcatca ataggtgtat ctactatttt tgatatatct attgctaatt
     1021 tcaaagaagg attatacttt cctgcttcta aacgcataat ggtttctctt ctaactccta
     1081 ctttttctgc taattgttct tgagttaatt cttttaattg tctatatttt tttaggttac
     1141 aagtgaattt aggcatatta tttttcctct aaaatataaa gttttatagc ataaacattt
     1201 aaagtgattg catatgtaat tgatagtaaa acacacatta attctatatt cctagacact
     1261 atagtaatac accataaaat gaataattct ataatagcaa gctgtcctgt aaatgccata
     1321 gctgcttttt catcttgtac atacctttca tctgctttat caccatttat ttttgctata
     1381 ataaagtttg agaaaaaagc aaaaaatcca atatatgtaa ggtcagcaac attgccagat
     1441 gaaaaatatc taagacttaa aaatccaagg aaaccacaaa aacccataaa aaattttttc
     1501 tgaatcattt tttatcctcc ttagtaattt taaaagagat atatttatat ctttatgata
     1561 caaatatatc tcttttatat tcttttgtca atagaaaatc agtattatac aatatttata
     1621 ctgcaccata attgttagag caaaaatcta atgcttgaga tgtttatgta tgactaaata
     1681 tgattttaat ttcaaattaa aagtagctaa attttatttg aatagagaag gaagagatat
     1741 tttaatagta aaacaatatg gagtttatga ccattctcaa gttgaaagat gggttaatat
     1801 attaggagag agtgaactaa aaataaaaaa attttctata ctatatggtt caagttagat
     1861 gttataaact atatgatgat aactaaatat agtgtacaat aaatcgctaa gtattttgga
     1921 ataaacaata gcacagttat agcaacatga aaataaaaat ttttacatag tggtgttgat
     1981 gccttttcta aaatgaaagg aagaccataa tgaataaaaa caaaaacact aaagaaaaaa
     2041 taaattaaca agagaacaag aattagaata tgaaaataaa tttttaaaag atgagcttga
     2101 gtttttaaaa aagatgtgtg tctcagggaa gccacattaa aaaacagtgg tatcattatt
     2161 aatcaaaaga aagttagatg tattatcaaa aaattagaac ttaaatgcat agcatttttt
     2221 cataaatcac ataaatataa ctcctataaa ggtataattg gaaaagtaaa aaataataaa
     2281 acaaatagaa gatttaaaac atcaatagca tatcagaaaa taacaaatga tatgctagaa
     2341 tttataaaat atatctaatg ataaaaatgg aaaatcaatt ataaaaaagg catatctcga
     2401 ttcatttttt gttatatagt caaataagaa tactatagat gaaagatacg attttgcata
     2461 tgtattaagt aagtattctg atgtttttgg ttgatacaca tgcaataaag aagttgtgtt
     2521 tgcagataaa ttagcaaaac tatagtataa gttgcaacaa taaagaaaat gaaaattatc
     2581 atactaaagc ataaggcatg ttacaatttt attagatagg acattaacat acactaaagt
     2641 actttttatt ataaagagta cttatttttt tgaaattcat taatgtataa actatcaaga
     2701 atattactca acataattta aaaacaactt gaataggtat ttttaaatta tgtgatataa
     2761 taaaaaatag aagtgtatgc caatacactt ctgtagttat aactaagcat tctattcttg
     2821 aggagtgctt ttttcatttt tataaccaac tctttttact ttaacaataa ttatcagacc
     2881 tatatattta tttttaattg tctgatttat tagataaaaa agttattatc atatttttat
     2941 catttggtat ttttattgaa ctaccataat cttcttctaa tttatctaga ccacttttac
     3001 ttatttctcc atctataatt atgtttaatt ctaagtattc ttcactacca ttagatgaca
     3061 ctaaagtgta cgaaccatta taaggcatgc ctaaattttc tattttatgt gccaaagggt
     3121 cttcatcacc aataccagag tttactgtta tttttccatc ttttttagtc caccatatta
     3181 tgtttccttc agtatatttt gattcatctg catagtagct aagagacgca ctttttattg
     3241 cacttatatc agcttcaagt ttagctatct ttgctttttc tatattttta aataatgctg
     3301 gaagtgctac tattgccaat attcctatta tagcaattac tactaataat tccactagag
     3361 taaatccttt tttatttttc atttatttcc cctccatata aaattaataa tatataaatt
     3421 tttattttgt taatttctac tttctatatt ttaccatata tagacaaaaa aatcactttc
     3481 tatgacaaat atactattat tttacaattt atggaaatga tttaaaatga catttcttgt
     3541 tagaaaagat ataagaaatg gattaaaata aagaaaacta cagtcatatc aatgtcaata
     3601 atattattta aaagcagatg aaaataaaaa gaggactcag aaaataatgt ctatttaatt
     3661 atacttactt accaatacca taacttgata taaacaagat tatactacat ttttttatgc
     3721 tctagtataa aatcatctaa ctgtgtatct ataaaatcaa aataattata tgaactatca
     3781 gtataataag tcaaaatatg tatacatgaa tacatatttt aaaagatata tattattaca
     3841 tacctaaaaa actttcataa aaacaaaaat aaaatatgct tttagtaact tgttaacata
     3901 acttataatt tattttataa gcattagcct atatagcttt gtgctactaa atctctttga
     3961 ttctaacgat ttagtgctat tctacaacta aaataataca atagagctat ttattgtttg
     4021 gtagcattgt ttgtaaatta aacaaattta tacatatgaa tattatttta cattcaaaaa
     4081 aacagactaa atatgtagaa tatttggtaa aacttacctc tagtttttgt cttaaaaagc
     4141 tcctattatt aacttaagtt agaagaatta aggaagtgac ataaattgaa agaagaaata
     4201 ttaatagaaa aattattaag agcagaatta atagtaatgg agtatttatg gaaaaaagac
     4261 actctaatgc ctaaaaaaga aattgtaaaa gaaataaaac agatttatgg atggcataaa
     4321 agtacaataa aaatactact aaaaaggcta gttgctaaaa ggtatttggc tagatatatt
     4381 ataaattctc aatctcatta taaaataata gatagtaaag aatattatac ttttaagaaa
     4441 aaggtactaa aatctagtaa aagcataaaa ataatgcgtt cacttaccac tacacataaa
     4501 aatgtaagta aagagaagtt agacctatta gatgagtatt atagaaactt aaaagaatga
     4561 tatagtaaaa atcgaatgta attatttacc ttaattcttt tgtttgtaaa actttattga
     4621 tacagaattt aacatataca taaattggta aaggaatgta tactatgaaa attagtaaat
     4681 taccagaagc ggaattaaaa gttatgagat atatttggga atcagaaaag gtattaatat
     4741 caagagaaat caccaagtct atggaacaaa aatatgaatg gaaagataca actatattta
     4801 cagtattaaa aagattacta aagaagagag tttttagaag cggagaaaat agataagtgt
     4861 acacactata atgtattagt aaaaaaaaat aatatcagag attttaaaca aaagaatttt
     4921 taaagaatac acataaaaat tcaatattaa gtttaatgtc agcactatgt agtaaggatg
     4981 atgaagtaga tggtgatatt ttaaatttat tagaagaata ttttaagaat ttgaaataag
     5041 aaaatgacaa attgatacat aat
//
LOCUS       DAMJZE010000228         4929 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJZE010000228 DAMJZE010000000
VERSION     DAMJZE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.232"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     misc_binding    193..424
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            532..2013
                     /locus_tag="PC541_003534"
     CDS             532..2013
                     /locus_tag="PC541_003534"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2840954.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2023..2916
                     /locus_tag="PC541_003535"
     CDS             2023..2916
                     /locus_tag="PC541_003535"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2840955.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3040..4101
                     /locus_tag="PC541_003536"
     CDS             3040..4101
                     /locus_tag="PC541_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2840956.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4256..4768)
                     /locus_tag="PC541_003537"
     CDS             complement(4256..4768)
                     /locus_tag="PC541_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2840957.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1840 a          607 c          760 g         1722 t
ORIGIN      
        1 tcattaaaaa cagcttcatc actatgaaaa tctaggtttt taaactttta aaaaagttaa
       61 aaaaatattt ttctaatact caatttgatt acaatttttt tacaatttaa aaaatatttt
      121 ttccaaattt ttatattaat acttgacttt gtctgtttta taaactagaa taattaataa
      181 tattaagaat attgaaaggc attgattagg aagagtaaat ctgaatcgat taccagagaa
      241 ggaaactact agctgaaatg tttccctaat tagaaggatt gaaaactacc taggagcttc
      301 tatccgaagt tatcttaatg attttagtag gaatagacgt ttttattact tgcgttaaag
      361 tatcaagtgg attttttaaa atcaatttgg gtggaaccgc gggaaacaga ttctcgtccc
      421 ttttttaggg atgagggtct ttttttatta cctaaattca gtttttaaaa tattctgtat
      481 ctaatttaat tagctaatta aaaatttaaa atatatggag gattataaaa aatgtattat
      541 tcaagtacga gaggcaccga agaaaaagta actgcatcac aagcaataat aaaaggaata
      601 tcaaatgatg gtggtcttta tgttccaagt gaatttccca atgtaaaaaa tgaacttata
      661 aatttagtaa atttaacgta ttctcagata gcattttttg tacttagtaa atttttatgt
      721 gattttactg aagatgaaat taaaaattgt atagaaaatg catatgatga aaagtttgat
      781 tgtagtagta tagctccatt aaataaagta aatgatactt atttcttaga actatatcac
      841 ggtccaactt tagcatttaa agatatggca cttactataa tgcctcatct tcttaaaaca
      901 tctataaaga aagataattt agagaaagat gttgtgattc ttactgctac atctggagat
      961 acaggtaaag ctgcacttga gggatttaaa gatatagata aaataaagat aattgtattc
     1021 ttcccagaag atggtgtaag cccagtacaa aaacttcaaa tgaaaactca aactggaaaa
     1081 aatacatatg tagttggtat aaaaggaaat tttgatgatg cacaatcagg tgtaaagaag
     1141 attttttctg ataaagaatt taatcaaaaa ctattggata ataattatat attatcttct
     1201 gcaaattcaa ttaatatagg aagacttctt ccacaagttg catactattt ttattcatat
     1261 atgaaattag ttagcaatag tgatataaat ttaaatgata aaataaattt tgttgtccca
     1321 actggtaatt ttggtaatat tctagctgga tattatgcta aacaaatggg tcttccaata
     1381 aacaaattaa tatgtgcatc aaatgataat aatgttcttt atgatttctt taaaactggt
     1441 gtatatgata aaaatagaac attgaaatta actacttctc cttctatgga tatattaatt
     1501 tcaagtaatt tagaacgatt actgtttgaa atatcaaata gaaatactga tattgtaaat
     1561 aagcttttat cagaccttag tgataaagga gtctacaaaa taagtgatga tatggaaaaa
     1621 aatttagcct cattctatgg agaatattca aatgaagaag aagttaaaag cacgataaac
     1681 aatgtgttta aaaattataa ttatttgata gatactcata ctgctgttgc atacaattgt
     1741 tatgaaaaat ataaaaaaga aacttctgat aacacaaaaa ctgttattgt aagtactgca
     1801 agtccattca agttttctga agatgttcta aagtctatag attgtgattt taaagacctt
     1861 gatgactttg ctataattga tagattatca agtatttcta aaatagatat tccaaaacct
     1921 attaaaaatc ttaaaaatgc agaaatacta cataaagata tttatgaaaa agatgaatta
     1981 aaactagcta taaaaaaatt cttaaaggta taggtgatta ttatgttaga gattatagtt
     2041 cctgcaacaa gtgctaatat aggtccagga tttgattgtc ttggaattgc gttaaatata
     2101 tacaataaat tttatgttga agaaattgaa agtggtcttg aaatagaagg ttgtgaagat
     2161 gcttacaaaa acgaaaacaa cttagtttat acatctatga agtacttttt tgatagagtt
     2221 aaaccagaaa aaatacctgc tggtatcaaa ataaaaatcc aaagtgaagt acctatatgt
     2281 agaggtcttg gcagtagtgc ctcttgtata gtagctggag ttatagctgc aaatgctcta
     2341 tcaggtgcta atttagataa aaatcaatta ctaaatatag cttcagaaat agaaggtcac
     2401 ccagataatg ttgctcctgc tattttagga aatatgattg tatctgttac tgataatgag
     2461 aatatacatt atgatattat aaaaattcct gaagaactta aattttgtgc aatgatacct
     2521 aattttaagt tatcaacaga aaaagctaga ggtgttttac caaaggaaat accatattct
     2581 gatggggtat tcaatgttag tagagttgct ttactaatat ctgccttact aaacaaaaac
     2641 tttgacctac ttaaagtagc ttgtcaagac aaattgcatc aagattatag aggaactcta
     2701 attgagaact acaatgatat tgttgaaaaa tctgaacaat taaatagtat tggagtattt
     2761 ttaagtggag ctggacctac tataatgtct ttaataaaag aaaatgatga tagttttgtg
     2821 gataatatga aaaattacct acaaaaacta aaatctgatt gggaaataaa agagttatgt
     2881 tgcgattcta atggagctgt tttaaatata atataattat cgtcttctat acaaaatata
     2941 actactcttt tattataatt tgtagtataa taattaggac taggtaataa tattaagtat
     3001 tataatctag ttctttaaac atgtttacat tgaggaggga ttatcataaa agagttaata
     3061 attagagatt tagttgcaaa agttcctata attcaaggtg gaatgggcgt tggtatatct
     3121 aggtcatcac tagctggtgc agttgctaag cttggtggtg ttggtgtaat ttctggagtt
     3181 caaattggat atgatgagga agattttgaa actaatacta taaatgcaaa tttaagagct
     3241 ataaaaaaac atatttctaa ggccaaagaa atctcaaatg gtggtattat tggtattaat
     3301 tttatggtag ctatgaaaga atatgaaaca tacgtaaagg aagctgttaa agctggtgta
     3361 gaccttataa tatctggagc tggattacct aataagctac catcattagt aaagggaagt
     3421 aatgtaaaaa tagcacccat agtttcaact gcaaaagctg ctaatgtaat tttaaaaatg
     3481 tgggatagaa aagagaaaac tactgctgac ttaatagttg ttgaaggtcc aaaagctggt
     3541 ggtcaccttg gatactctaa cgaggaactt gataacatag attctataga ctatgataag
     3601 gaatttgttg aaattttaaa ggttgcaaat acttatggag aaaaatttgg tagaaacata
     3661 ccagtagttg ctgctggtgg tataacttct agtagtgatg ttaaaaaata tatagatatg
     3721 ggtgcaagtg gtgtacaagt tggtacaaga tttgttgcta cttacgaatg tgatgcccat
     3781 gagaacttta aaatggcata cataaatgct agtgaagaag atgttcatat agttaaaagt
     3841 cctgtaggtc ttcctggacg cgccataaga aacaaattta tagaagaagt taaaataaat
     3901 cgtccagaaa taaagaagtg ctataattgt ttgattcctt gtaatccaaa agaaactcct
     3961 tactgtattt ctcaagctct aatcaatgca gttaaaggag atgttgaaaa ttcactttta
     4021 ttttgtggaa atgatgccta taaaattgat aaattaagta ctgttgaaga tgttatcaat
     4081 gaattgattt ctgaattata atttaaagaa aactaaatta aaattttatc aagctaagga
     4141 gtttaaatac gttatttatt aatctttata aacatattta tgtccttagc ttttttgtgt
     4201 caatttcctt ttatggaaat tttaatttaa ttagagatag tattaacctt tataactaat
     4261 cttcttttac aagtacattg gttatttcag ctatatataa agtatgataa tctttttgtg
     4321 gataccatct tccatctata ccttcagcaa taaaacattg aggttcaagt ttctgactat
     4381 acattttttt gcatataata gccattttag attctttaaa ataaggtgta tcatttaagt
     4441 gttctatatt aaatccaatc tgtgatatct tatcctcttc tctaccagat acctttccac
     4501 aatatgaaag ctctttacga aaatcttcat caaaaaatgt aagagaaaat ttatctgtat
     4561 tatctataaa ttccttagta taacgctgtg gtcttattac tacaaaagca acatcttttc
     4621 cccacataac tccaagccct ccccagctag cagtcattgt attcacttta ccttcatttt
     4681 ctgctgttat aagtgtccaa tctttaccta tcaatccaaa tggattattt ttaatctctt
     4741 ctgccaaaat ctctttaaaa tcactcatat ctctaataac ctctcattct cttatattta
     4801 tattcctttt attttcgtga taaattataa atgaagtcta gttttgaaaa taaaatactt
     4861 ttttaaatat attattgcaa ataagtaaat atatatatac tctcttattt tttaatatat
     4921 ttaataatt
//
LOCUS       DAMJZE010000229         4898 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJZE010000229 DAMJZE010000000
VERSION     DAMJZE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.233"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            51..854
                     /locus_tag="PC541_003538"
     CDS             51..854
                     /locus_tag="PC541_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840958.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            866..1996
                     /locus_tag="PC541_003539"
     CDS             866..1996
                     /locus_tag="PC541_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2840959.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            2000..3409
                     /locus_tag="PC541_003540"
     CDS             2000..3409
                     /locus_tag="PC541_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2840960.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3620..4129
                     /locus_tag="PC541_003541"
     CDS             3620..4129
                     /locus_tag="PC541_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2840961.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2037 a          416 c          855 g         1590 t
ORIGIN      
        1 tattataatt aatatagata taaaaataat tatagaagga aaggcacatt atggaaaaat
       61 tgagagttat tgaaatgata aaaaataatc ccaatattat agctacaata gataatccaa
      121 ctgatgaaat gaaattgtta gctattaaag aaaatgggct tgtactagaa tatataaata
      181 atccaaccag agagatgcaa gaactagcaa tagataataa tattagagca attaaattta
      241 tagataatcc aacagaagat atgatgataa aagcagtaaa tgagggatgg agtatactgg
      301 attatatcaa aaacccaact gataaagtta ttgaaatggc tataaatcaa gcaggatggg
      361 caattaaata tgctaaaaat ccaagtgaag aattacaact gttagcagta agaaaaaatt
      421 atgattcgat aaagtttata aaacaacctt atgaaagtgt acaagaggag gcagtgagaa
      481 taagttatga tgccttgaga tatatagatt cacctagtta taatgcagaa cttatagcta
      541 taaaaaataa tgaagcagct attagcttta taactgattt agataaaaat aaaatgttgg
      601 aatttttaaa agtgaatatt ttagtgatta agtacattct gaaaaaagta tctaaagatg
      661 aattagagag tgcattaaaa gaagtattat ccaaagaaga tgttgaggaa aagtatatta
      721 gagatttttt gaattgtagt gttatagata gaaatagcga aagtatacca atagataaaa
      781 ttatgtttat ttataaatat ggaagtaaga cagcaaaaaa aatagctgta gatgagaaat
      841 taaagattat gtaggagaga gaaaaatgaa ttttatagac acattaaaaa gcgttgactt
      901 aatattatcc actggagaag tacctttaat agtaggtgaa agtggaatag gaaaaacagc
      961 tttagcaaat aagcttgcca aagaaaatga ttggagttta attgttattg atggaaactt
     1021 acttaaagag ggtgaaatag gtggtcttcc aactatagaa tcttatgtag gagttaattc
     1081 taatggatat aagactgaaa agaaaacaac agtgtatgct gttcataaca aattaagaga
     1141 aattgatgaa gagatatcta aaagtaagac agttctttta tttatagatg agataaatcg
     1201 ttgtgagcat acagtacagc aagaacttat gaatttaatt ttaaatagag aaattaatgg
     1261 atataaactg catgatgatg taaagatttt agcagcaatg aatccatcaa gtaaatatgg
     1321 ttcagatttt gattatcaag tggtagatat ggatgcagcc caagaaaata ggtttgtttg
     1381 gttaaatatg gagagtgacc atacacaatg gataaaatgg gcaatagatg agggtattga
     1441 aagaaaagtt atcgagttta tttccacttt tccagaatat ctgcataaaa taaatgaaga
     1501 tgatgtaaga gccactccta gaagttatga gagagtatct aaaatttata aagtttataa
     1561 agaaaaaaat aattctattc ctagagcagt atttttaaat gttgtaaagg gtaatgtagg
     1621 taaagttatt gcagaagaat ttataagttt tattgaatca aactctgaac cattaatatc
     1681 ctatgaagat gtttttttag gagaatctat tgatgagtct attgtagaaa gagtaaaaaa
     1741 tgagagtcat acaagacttt atctatcagc tatgaacatt ctaaaagact tagaattaaa
     1801 tattaagaat gataaatatg aatcaaacca ctatattaat agatttattg aatttttaaa
     1861 aatgtatcct gtagatttaa tgataggtat tatgaaggat attagaaaca gttatattga
     1921 agtatataaa aaagctatag aaaatgaaga gtttgtaaaa tcatattttg aatcttatag
     1981 tttaataagg ggataatgta tggaaaatta ttttgataaa caagcaaaag agctttataa
     2041 taaagcaagt gaaattataa atactcattc tatgctaaag gcaaatcgtg ctaatgagaa
     2101 atttgatata gatataccac aagattttaa gtatgagttt tttagtcttg tagataaagt
     2161 gaatttaagt cttatggaag aagaagataa tttttatgga tattttttgt ttcagatgtc
     2221 aagagaaata agatttgata taagtagtcc tacaggtgtg aattttaagg gagctaaata
     2281 tattatatat tttaatccaa taatcttttt aacgcttgac ataaagcaaa tggagactac
     2341 aattaaacat gaaattcatc atatattatc tatgcatttg atgagagcaa aggagttaaa
     2401 aggaaagtat agtacattag caattaatat ggcaatggat atagtagtaa ataaatttct
     2461 aaacaattta ccaccatatg caactacctt agagtgggta aatttaaaat attccttaaa
     2521 acttgaacca tatgaacctt ttgaatatta tgtagaaaag attcagactg aattagattt
     2581 gatggaagtg gatgaagatg gtgaggaaga tgatagtgat aaatatggag atatagaaat
     2641 tgattatagc cccgaaagaa ctcatgatat atgggatgaa tccaatgaga tagatgaaaa
     2701 tacccttaga gaatttactg aaaaatttat taacagttct caaaaaggta aagttcctgc
     2761 ttatttagaa agtatgatat catcacttaa aaatagtaag ggtgaattgc catggaattt
     2821 atatcttaat agaataatgg gaacagttga aaataataaa aagaagacta taacaagaag
     2881 aaatagaaga caaccaaatc gtttagattt gaggggagaa cttagaagtc ataagtcaga
     2941 aatagcagtt gcacttgatg taagtggaag tattagtgat gaagagttta aacaagctat
     3001 taaagaagtt cttagtatag tgaaaaacta taatcatgaa attacaatta tagaatgtga
     3061 taatgaaatt agacgtgtat ataaagtgag aactgaaaaa gatataaaag atagaattag
     3121 gataggtgga ggtactagat tttctccagt ctttgagtat gctaataata aaaagattaa
     3181 tttgttagtc tactttactg atggtaaagg tgaaaataaa ctacaagtaa tacctagagg
     3241 gtacaaaatt ttatgggtta tttctggaag aggagataaa atttcattaa aagaacctta
     3301 tggagcagtt aaaaaactta ctaaggttaa aacaaatgat gaaatattag aaataaatga
     3361 tgtaagaaat gatgggtact caatgaatag ccaagcacca attttatagg tattcacagg
     3421 agataagctg tattaattta ttttgatatg cttgttataa aaatctaaaa aatacattaa
     3481 tcaatggtta atgtattttt taattttaat aatataagtt ttacttacat ttaagtctca
     3541 aaatgtgtta aaatataata tatagcagat agttttggca aggtggattt agttgtcaaa
     3601 actatttttt ttattaagga tggtgatgtt tttgtttaag attgatgatt atataatgta
     3661 tggtatgaca ggagtctgca aagtcatgga tataacaaat gaacgattta caaatggaat
     3721 aaaaaaagag tattatgtat tgagtcctat ttattctaat aatacagtaa taaaaattcc
     3781 tgtagataat gaaaaagtac caatgagaaa gttactttct aaaattaatg tgctttcatt
     3841 aataaatgat attcctaata tggatacatc atggatagat aatgaaaaat tgagaagtga
     3901 gcaatttaaa aaaatactca gaggtggtaa atgtgaagag ttaataaaat tagtaagaag
     3961 tatagataat aacagagagt atgtaaaatc tattggtaaa aaaacacatc aagcagatga
     4021 taatataatg aaagaagctg aaaggttatt aaatgaagaa tttgcaacta ttttagatat
     4081 ttctcctaat gaagttagtt catatatttc aagtcatata cctcaataaa gtgttttagt
     4141 atcctttatt taatctattt taagtattat ttatgtatgc tggttttggt aaaataaaat
     4201 atataattaa attatataaa aaataataat gattgtattg aagaattaca gaatagtgcg
     4261 gagagcctac tcctttaggt gtgagatgga tatcacttaa agatttaaat tgttattgta
     4321 gatgttttta atataaaaca agattaataa gtgtattgta agctttaagt gaaattgata
     4381 ctataatgag ttacttagat tttaaaatat ataatattat aaaaaatcct tttaggtttt
     4441 gaatgaataa gatggatact atgttgaata gaatccatct tatttaagtt ttatttatgt
     4501 tgtttatttg taagcattat ttttgtacat gaaaacacag tgtaaaaata tgcttagaat
     4561 tatgaagttt tattaaaaag atattcgaca aaataagact aaaaatattc ttaaacttat
     4621 aactaagata aatattgtat atttatagtg tagcctgaaa aattgtggat gtacgaaatt
     4681 tatttagtat tttattgaaa caaagtagaa gaagtactat agtttatctt tgaatagatt
     4741 gttatttttt agtgaaaaag ttattaggtt aatacataaa tataatagct tataaatagt
     4801 ttaagcatgt taaaaaagta gttattagag atatatagtt acattgctta taagatactt
     4861 aaaatgatag cattcactta tattcagaag attaagat
//
LOCUS       DAMJZE010000230         4869 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJZE010000230 DAMJZE010000000
VERSION     DAMJZE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.234"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     misc_binding    388..626
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            690..2609
                     /gene="thrS"
                     /locus_tag="PC541_003542"
     CDS             690..2609
                     /gene="thrS"
                     /locus_tag="PC541_003542"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2840962.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3377..4468
                     /locus_tag="PC541_003543"
     CDS             3377..4468
                     /locus_tag="PC541_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2840963.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1923 a          477 c          858 g         1611 t
ORIGIN      
        1 atttagatat taattgtatt tatttatttg attgtgttac ttattaatag tatttaagta
       61 tttactgtac tacttattaa ccatattaag tattgattgt atttaagtat acaaaaccta
      121 aaaagagtac aaaaattaga agttagtttt aataaaaatt agttttagta tttatctagt
      181 catatctatc atgcatgtgg atacgtttta aaaattttaa atttatgaaa atattataat
      241 ataactataa atcaagagat taatttaata taaaaatata agaatattgg tttacagctg
      301 attgagatga aaaataatgg gtaattaata tattacatta atgttgattg atgtataact
      361 atctgttata atgaaaatat attaaaaatc atagtgttga agaggaagag tagagtagaa
      421 accattctca gagaatttgg gtctatggct gagagcccat gtaataggtc tatttgaaaa
      481 ctaccttgga gctgagctta ttagcagtat ttgtatttat actatacgtg aaagtgctcg
      541 ggtgacgacc ttatagtcat caagagggtt gtatatactt gtatacgcca acttgggtgg
      601 aaccgcgaat taatatcgtc ccaaacatat ttggggcgat tttttattac aaaaaatcta
      661 aaaattattt caataaggag gaaaaaacaa tgattaaagt tgctttaaaa gatggctcaa
      721 taaaagaatt tgaaaatgct atttctgtta tggatgtagc taaatccatt agtgaaggat
      781 tagctagaaa tgtggtagca gcgtctgtga acggagaggt tgtaggtctg gaccacatta
      841 tagatactga ttgtgacttg aatttattta agtttgaaga taaagaaggt aaggaagttt
      901 ttagacatac atcagcgcat atattagctc aagctataaa aagactttac ccagaagcaa
      961 agcttgctat aggaccaagt atagaaaatg gattctacta tgatatagat ttagaccata
     1021 gattagttcc agaggaccta gagaaaatag aagctgaaat gaaaaaaata gctaaagaag
     1081 atttaaaaat agaaagattt gaacttccta gaaatgaagc tttagagctt atgaaagaac
     1141 aaggagaaga ctataaggta gaacttattt ctgatttacc tgaaagtgaa ataatatctt
     1201 tctacaagca aggtgatttt actgatttat gtagaggacc acatttacca tctactaaaa
     1261 aggtaaaagc tgttaaatta caaagtgtag caggtgcata ttggcgtggt gacgaaaata
     1321 ataagatgct tcaaagaata tatggaactt cttttgagaa aaacaaagac ttagaagagt
     1381 acttacattt actagaagaa gctaaaaaga gagaccacag aaaattaggt aaagaattag
     1441 gattatttat gatacctgaa gaaggtccag gattccctat gtttttacca aaaggtatgg
     1501 aacttaaaaa tgaattatta aaattctgga gagaaataca cagaaaagct ggatatattg
     1561 agatagaatc tccaatcata ttaaatagaa aattatggga gacttcagga cactggtatc
     1621 attacaaaga aaatatgtat acagttaaaa tagatgatga ggattatgct ataaaaccta
     1681 tgaactgtcc tggtggatta atttactata actctcaact acattcatat agagatttcc
     1741 caatgagagt tgcagagctt ggtagagttc atagacatga gttatctggt gcattacaag
     1801 gattaatgag agttagagca tttactcaag acgattcaca tatattcatg ttaccagagc
     1861 aaattaagga cgaaataaaa ggagtagcta acttaataga tggtatatac aaaacttttg
     1921 gatttgaata taatttagaa ttatctacaa gaccagagaa ctctatggga agcgacgaag
     1981 agtgggaagc agcagaaaat ggattaagag aagctttaga agagttaggt ctaccatata
     2041 ctattaatga gggtgatgga gcattctatg gtcctaagat agacttccat ctaaaagatt
     2101 gtttaggaag aacttggcaa tgtggtacta tccaattaga tatgcagctt ccaagacaat
     2161 ttgataatac ttatattggt caagatggtg aaaagcatag accagttatg attcataggg
     2221 ttgcttttgg tagtatagaa agatttatag gtatattaat agagcattat gctggtaaat
     2281 tcccagtttg gttatctcca actcaagtta agatacttcc tatatctgat aaatttatgg
     2341 attatgcaaa tgaagttaag aaagagttat ttgataaggg tataagagtt gaattagatg
     2401 atagagctga aaaaattgga tttaagataa gagaagctca gcttgaaaaa gtgccataca
     2461 tgttaatagt aggtgaaaaa gaagtagctg ataataatgt ttctgttaga tcaagagata
     2521 aaggtgaaat aggtagtatt aaattagatg aatttatagc tagtatttca aaagaaattg
     2581 aatcaagaga aagtattatt caagactaat atatattata ttagtagcta agatattgtt
     2641 atataagata agtttaatca atatgttgat atgttgatgc atagtgatta aatttatgaa
     2701 aagatgagta taattagagg tacagttaat catattttta tgattaatgt acctctagtt
     2761 ttttgtatgg tagatttatt aataaatata tcatagaagt gttttgaaca tggaaatatt
     2821 tatgtgcatt ctttaaataa aagtttattt tttgagtgat aattaagtgc ttgttggtaa
     2881 ggttatggct gtaaaaatca taactttatt ttcatatttg ttccattttc ataaaaatta
     2941 ttatcccttt aagaaaattt cttaatcctc ttgaaatatt tcctttttag tctaaagtta
     3001 aatttctttc ttcaaaatga atatttaaat gaactaaaat ttaagtacat tttctaatct
     3061 tatgagtcta tttttcatag ataattgccc tataatagaa gaatctatgt tggtgctatg
     3121 tggtgacaat aacagcattg tcagtgatta attaatgctt tataaatgat taattagtgt
     3181 tttatgttat ttttggactt atcctctata catagattaa tttatttaac tttagataga
     3241 gagagaatgt caagtacata cacagacatt agagtttaag aaagtgttta tggagcaaga
     3301 acttttaaaa gaggtagtga cttagtttaa tttcaagaaa tgataaatac aaaagaggaa
     3361 aaagagaggt gttgaaatga tagaaaaagt agaagaaaaa tctaagatta ttaaaacatc
     3421 aaaagaatat aagcgaacta taattgaaga aaaagataaa gaatggacca aaaaattaaa
     3481 tgatatactt agtagaagta gtttttctaa tgatgaatta gataaaatca aggcattaat
     3541 tccaaaagag attttgacta atgaaaatgt tggagaagtt gtgactgaag atggatatgc
     3601 taagcctgag tatgatgagg tatttaaatc attgttcact ttaaataatg aatatgattt
     3661 acttgtaaat tttataaatg atattctaaa agatgctcca tatgctaaca ggaatatcaa
     3721 accgttcact caaataaaga gaataattag agttgaaact gatccaacaa ttaattacat
     3781 tggagagaaa agacctaaac ttgatatttt ggctgaagat gaagaaaata atcatataaa
     3841 tattgagatg caaagagctt ttgagaacga ttatttggaa agagctgaat attatcttag
     3901 tcgtgtacat ggtagaaagt tagaagaagg taaagagtat aaagaaattg gaaaaactat
     3961 tggaatacat atactaaatc atgtaaaata taatcacata gaagattatg taaattgcct
     4021 gagattaaca atggacgggc atccagatat ttattctagt aaaactgcat tatattttat
     4081 agagcttcca aaaattcata aatctgacta tatagtaaat agaattatgc tatggggaaa
     4141 acttattagt aatccttcta acttagatgt aagagttata gctaagaatg attctattat
     4201 taaaaaagca cttgatagat taaaagaatt aggtagcaat gaagaatatt tattaaatct
     4261 gaaaagaggt gcttatatca tgaataaaag tagaaatttt aaagaagaga ttgaaagaga
     4321 gacagcaatt agagttgcaa aagaaaaaga ttataaaata attataatgt tgaaaaaaaa
     4381 tggttttaaa ttagatgaca tattaaataa atttaatgat tttgacttaa gtgaagatga
     4441 gataaaagaa gtatatgaaa ataattgatg ataaataaaa agtgaactta tataagtttg
     4501 ctgaatcgga gtctggaaat atttatatgt attttttaat gaatgaaaat ttattttaaa
     4561 aacaataatt tagaagtaca gttaattata tttttatgat actgtacttc tagttttttc
     4621 tattgtagat ttattattta ctaataaatc tacaatagaa atgcttttgt tgtaagaatc
     4681 attttatcta atgattgttt tttataatag ttaaatttcc atttgtagtg tttaaatata
     4741 aatctataac agcatttcta taaatgttat aaatcactat aatgattcaa ctcaaagtta
     4801 ctgcttaaag aagcagagat tttatcaagg tatatcatgg aaaaaaatat tttattgtaa
     4861 tatttataa
//
LOCUS       DAMJZE010000231         4779 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJZE010000231 DAMJZE010000000
VERSION     DAMJZE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.235"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..1777
                     /locus_tag="PC541_003544"
     CDS             <1..1777
                     /locus_tag="PC541_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2840964.1"
                     /translation="LYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNKDTYVYN
                     NKTASELVKMLTKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLREKKEMYV
                     LYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEESGKRDV
                     YIAQDSAHMRSWGILQMFETVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKNVLGDIR
                     VRAGCLIPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYSSSSYSS
                     GNANNKDEKKNGPAQNTTSKEDNDMINKLNKVFKGKLSNTGNIFVKYSNAYKVNPALM
                     AAISMHESARGTSNIANTKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHIGRKTLES
                     IRDKYAPLYDSPLNKDWVPGVGKFYKQITGNAYSSNSAGTGVGSNEEAEKNLKDTTYQ
                     VQNSNNANTSTNNNSKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFKRGAGI
                     NLKRVSADQSKDSRGKLLCNINDVKAGDLVFFKNEQGKVHHVGLYIGNDQYIHAPQTG
                     DVVKISSLSGRQKKKHDFARARRFF"
     gene            1791..2051
                     /locus_tag="PC541_003545"
     CDS             1791..2051
                     /locus_tag="PC541_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2840965.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLVVKIEQKA
                     SFEEFFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            2056..2475
                     /locus_tag="PC541_003546"
     CDS             2056..2475
                     /locus_tag="PC541_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2840966.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLHIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNIKGKVHCKFMVHTKYGNIKAEKVVSV"
     gene            2476..3525
                     /locus_tag="PC541_003547"
     CDS             2476..3525
                     /locus_tag="PC541_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2840967.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDIGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            3518..4135
                     /locus_tag="PC541_003548"
     CDS             3518..4135
                     /locus_tag="PC541_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2840968.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFNEVHTFVSSIKPCNMSLDYTRVLVSKDNG
                     MYFGATTVTGHEITIYPWSPSDIETQGEIKIMSGNGFGYQEITIY"
     gene            4151..>4779
                     /locus_tag="PC541_003549"
     CDS             4151..>4779
                     /locus_tag="PC541_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2840969.1"
                     /translation="MATDKSYYTILTDIGKAKIANASLVGEKVDFAKIQLGDGGGNEY
                     NPTEEQTALKNVVWEGNVGNVKTDEAMANCLILESLIPANAGGFIVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     IDTTKTELTSNIEIAKTELNNKIGDTTLLETTDKTSLVGALNEVKTSVD"
BASE COUNT         1958 a          530 c          933 g         1358 t
ORIGIN      
        1 gctgtattat aagggaatag gtatatttta tggatatata tttcaaaaga aaagagataa
       61 agaaaatcat attaaaattg ttgcttacga ccagttgaga tattttaaga ataaagatac
      121 ttatgtgtat aataataaaa cagcaagtga acttgtaaag atgctaacta aagattttaa
      181 cttaaaatac aatgtcatag aagacacaaa atataaaata tcgagagttg aagaaaataa
      241 aactttattt gacatgatac taacagcact agatgatact ctaagagaga aaaaggaaat
      301 gtatgtactc tatgatgatt ttggaagaat aacattaaag aatgttgcat caatgaaatt
      361 agatactgtt atgaataatg atgtcataga ggattttgac tataattcaa gtatagatag
      421 tgatacttat acaaaaatta aactcgtcag agacaacgag gagtcaggaa aaagagatgt
      481 gtatattgct caagactcag cacatatgag aagttgggga atacttcaaa tgtttgagac
      541 agttgataaa aacatgagtg aagcagagat aaaacaaaag tgtgatatac ttttaaaatt
      601 atataataag aaaactaagt cattaagttt aaaaaatgtg ttaggagata ttagagtaag
      661 agcaggttgt ttaatacctg tttttttaga tttaggagat attgagttac aaaattatat
      721 gttagttgag aaagtaaaac atacatttga aaataattca catttcatgg atttaactct
      781 tgttgatgga gatgaatttg cttcttattc ttcttcaagc tacagtagtg gaaatgcaaa
      841 taataaagat gaaaagaaaa atggtcctgc acaaaatact acaagcaaag aagataatga
      901 catgataaat aaattaaata aagtatttaa aggtaaatta tcaaatacag gaaatatatt
      961 tgttaaatat tcaaatgctt ataaagttaa tccagcactc atggctgcta tatctatgca
     1021 cgaatcagct agagggactt caaatattgc aaatactaaa aataatttct ttggaatgaa
     1081 aggaatgtct tttggctctg tagatgaagg aataaaaaga ggtataagta atttatcaag
     1141 aaattatatt catataggaa ggaaaacatt agaaagtata agagataaat atgctccact
     1201 ttatgatagt cctcttaata aagattgggt accaggagta ggcaaatttt ataaacaaat
     1261 aacaggaaat gcatatagtt ctaatagtgc aggtacagga gttgggagca atgaagaagc
     1321 agaaaagaat ttaaaagata caacttatca agttcaaaat agtaacaatg ctaatacatc
     1381 aacaaacaat aatagtaaag taagtaaagt tattcaagaa gcaaaaaatc aacttggcaa
     1441 gccttacaaa tggggtggta atggtccaaa gagttttgac tgtagtggtc ttatggtgtg
     1501 ggcatttaaa agaggtgcag gaataaatct caaaagagtt tcagcagacc aatcaaaaga
     1561 tagtagaggg aaactattat gcaacataaa tgatgtaaaa gcaggtgatt tagtattctt
     1621 taaaaacgaa caaggaaaag tacatcatgt tggactttat ataggaaatg accaatatat
     1681 tcatgctcca caaactggtg acgtagtaaa aataagtagt ttaagtggta gacaaaagaa
     1741 aaagcatgat tttgcaagag ctagaagatt cttttaagcg aggtgataaa atgagccaag
     1801 aattattgca aataatcaag aaagcagcaa tggatgcagt agaaacaagt aatccaatgc
     1861 aaattgcatt tggaactata gaaagtgtta atcctttagt agttaagata gagcaaaaag
     1921 catcttttga agaatttttt ctaatacaaa cagatacatt caaaagatat acagataaaa
     1981 aaatagggga taaattggtt ttaattcgta tgcaaggagg acaacaatac ttgattttag
     2041 ataggatgtg ataaagtgtt accaacagat aacattgact atgatattga agatgtatca
     2101 attataaatt ttgatgtaag gcaagaacca agtaagacat ttaaacttca tatagaaaaa
     2161 aacagagtag atggtatttg tgatgatgtg gaagcattaa aacaaaccat ctttttgatt
     2221 ttaaatactg aaaggtatga gcatttaata tacagtagaa attatggagt cgagttgaac
     2281 gaccttattg gagagcctat atcctttgta atacccgaac ttgaaagacg aatcaaagaa
     2341 gcactaattc aagatgatag ggttgaaaat gtagataatt ttgagtttca aaatataaaa
     2401 ggaaaggtcc attgtaaatt tatggtccat accaaatatg gaaatataaa agcagagaag
     2461 gtggtgagtg tataattgtt tgagttaatg acatttgaaa atataattaa aagaatgtta
     2521 gatagtgtac cagatacttt tgataaaaga gaaggttcta taatatataa tgccttggca
     2581 ccagttgcta tagaacttac agaaacatac attgcaatgg atgaattact agaccaaaca
     2641 tttgtagata ctgctagtta ttactattta gagaagagat gtaaagagag aggaattaca
     2701 cctttgccag ccactaatac gattgctaaa ggcgttttca atatagatat tccacttgat
     2761 tctaggttta atttaggaga gtataactat gtagcaattg agagaatatc tgaaggtata
     2821 tataagatga aatgtgaaac tgctggacct atttttgaac ttggtcagtt gattccaatt
     2881 gaatatatag acggtcttga aacagcagag ttaacagaaa tactgataaa tggagaagat
     2941 gaagagagcg aggacagttt aagacaaagg tactatgata gtttaaattc acaaagtttc
     3001 ggtggaaata tacaaaacta taaggatgaa gttaacaaaa tacaagatat tggaggagtt
     3061 aaagtttatc cagtttggaa cggtggaggt actgttaagt tagtaataat taattctaat
     3121 ttcaaagtac catcagagga tttagttaat ttagtgcaag aagaaattga cccaattgga
     3181 catcaaggac aaggcttagg attagcacca ataggtcata gagtcacagt tgaaggagtt
     3241 acaagtacaa ctataaatat atcagcagaa ataacatata agagtggata tacatgggag
     3301 aatataaaat caattgcaga agaagcaata gacgactatt taaatgaact taacatgagt
     3361 tgggaagatg aagaaaactt aatagtccgt atatctcaaa ttgaaactag attacttagt
     3421 atagatggag tgttagatat tacaaacaca atgataaatg atgttaaatc taatctaaca
     3481 atagatagta acagtatagt agtgagaggt gaggtagttg gataaagaga ttaatctaat
     3541 aaattacttg ccacaaattc tacaagataa agaagaatat ataaaagtat ttaatgtaga
     3601 aaataaagaa ataaaaacac tacatgataa attaaaggac ctatcaaatg accagttttt
     3661 agaggaccta actataagtg gtataaaaag atgggaaaag ataatgtcta taactcctaa
     3721 aagtaatgag agtttagaag atagaaggtt taggattttt agtaaatata taagtaaatt
     3781 accttactca gagagatttt taaggaactg gctagataat gtagttggag aaggcaatta
     3841 tgaattaact attaataatg ctacttataa catacacctt gagagtgatg ctagaaatca
     3901 agattggttc aatgaggttc atacatttgt aagtagcatc aaaccttgca acatgagtct
     3961 tgattatact agagtgttag ttagtaaaga caatggcatg tattttggtg caacaacagt
     4021 cacagggcat gagattacaa tttacccttg gtcacctagt gatattgaaa cacaaggaga
     4081 gattaagatt atgagtggaa atggatttgg ataccaagag attacaattt attagatagg
     4141 aggtggtagt ttggctacag ataaaagtta ctacacaata ttaacagaca taggaaaagc
     4201 gaagattgca aatgcaagtt tagtaggtga aaaggttgac tttgctaaaa ttcaactagg
     4261 tgatggtgga ggaaatgagt ataaccctac agaagaacag acagcactta agaatgtagt
     4321 ttgggaaggc aatgtgggaa atgttaaaac tgatgaagct atggcaaatt gtctaatttt
     4381 agagagtctt atccctgcaa atgctggagg atttatagtt agagagatag gttatttaga
     4441 tacagagggt aatttacttg ctatatcaaa gtataggtca gcatataaac ctaaagtgga
     4501 agatggagca gtaattgaca tgaaggtaaa aactatcttt gttgtatcta atgtaaataa
     4561 tatagaactt aagatagacc caacaataat ctttgccaca ttaaaagatt tgcaggacct
     4621 ggacactaaa attgatacaa ctaagacaga attaacaagc aacatagaaa ttgctaaaac
     4681 agagctaaac aacaaaatag gtgatactac tctacttgaa acaacagata aaactagtct
     4741 tgttggtgca ttaaatgagg taaaaactag tgtagatag
//
LOCUS       DAMJZE010000232         4732 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJZE010000232 DAMJZE010000000
VERSION     DAMJZE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.236"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            160..1311
                     /locus_tag="PC541_003550"
     CDS             160..1311
                     /locus_tag="PC541_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2840970.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1357..2532
                     /gene="thiI"
                     /locus_tag="PC541_003551"
     CDS             1357..2532
                     /gene="thiI"
                     /locus_tag="PC541_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2840971.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2688..3491
                     /locus_tag="PC541_003552"
     CDS             2688..3491
                     /locus_tag="PC541_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2840972.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3734..4375
                     /locus_tag="PC541_003553"
     CDS             3734..4375
                     /locus_tag="PC541_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2840973.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1900 a          454 c          917 g         1461 t
ORIGIN      
        1 ctataaaaat ttgtaataaa gtgaaaaaac tagcttttca tgaaataata ttttataatt
       61 taagattgtc tttttaataa agattaacaa aaaaaatact ataattagtg gtataatagt
      121 tagagttgaa gattttaaat aaaggtaggt gtaattaagt tggaaatata cttagacaat
      181 agcgcaacta caaaacctta tcaagaagtt atagataaaa tggtttatgc acttaatacg
      241 gaatatggaa atccttcttc tgtccataga aaaggtgtag aagttgaaaa agctataaaa
      301 gaagtaagac aagatatagc taaatctcta ggagcgaaag aaaaggagat ttattttaca
      361 tcaggtggta cagagtgtaa taatactata ataagaggta ttactagttt aaataaaaaa
      421 agaaaaaatc acataatatc tactaacata gaacatcctt ctgttttaaa tacattaaaa
      481 gatttagaag aagatggttt tgaagtaact tatttagagg tcggaaaaga tgggaaaata
      541 aatatagagg atttaaaaaa tgcgataaag tcaactacat gcttagtaag catgatgcat
      601 gttaacaatg aaataggaac tatacagcca ataggtgaag ttggaaagta tttgaagggt
      661 ttaaaagaaa aaatttattt ccatgttgat gctattcagt catatggaaa aattaacttt
      721 agaccatcta aatacaatat agattttatg agtgtaagtg cacataaatt tcatggtcca
      781 aagggaattg gatttatgta tataaaagaa aataatcgcc ttaagcctat gttaacaggt
      841 ggtggtcagg aaattggaat taggtcggga acagaaaatg tacctggaat atatggtctt
      901 ggagaagctg ttagaatact aaacaaagat ttagatgctg ttatttcgaa agtagataac
      961 ttaaaaaata tattaaaaaa tgaaataatt gataatatag aagatataaa aatcaattct
     1021 ccagaagacg gagtatgtca tatattaaat gtatcattta gagggacaaa aggagaagtt
     1081 ttacttcatt acttagagca aaaaggtatt tatgtatcaa ctggctctgc atgttcttct
     1141 aaaaagaaag gtagttacgt attaaatgct ataggtctta caaatgaaga aataaatgga
     1201 acaataagat ttagtttatc tgatatgaat acagaagaag aaatgttaga agctgtgaaa
     1261 gttttgaaag aatcaatttg tgacttaagg tcaataatga aaagaaaata aagaatactg
     1321 tataaatagt gataataaat tagtttaaga ggtgaaatgg tgtataatat attaatagta
     1381 aagtatggag aaattggagt aaaaggaaag aatagatata tatttgagaa taggcttata
     1441 agaaatataa gaaatatgtt aaaaccaatt ggaagattta atgtatataa agagtatgga
     1501 agaatatatg ttgatttaga agactatgat tatgaagaag taattgaaga agttagaaaa
     1561 gtatttggga tagtgggtgt gtgccctgga gtaagagcta aaaaagatta tgatacatta
     1621 aaagaaatag ctttaaaaat gttagaagaa aaaatagagg ctggatataa aacttttaag
     1681 gtagagtcaa gaagaggaga taagtctttc ggattgactt ctcaagaaat gagtatggat
     1741 ataggtggat acttattatc aaaggtagga gataggatta atgttgatgt aagaaatcca
     1801 gaagttaaaa taaagtgcga atacagagaa tttcatacta tggtctatag tgatactata
     1861 cctggatatg gtggattgcc acttggaacg aatggtagag ctatgtctct tttatcagga
     1921 ggtatagata gcccagtagc tacatggatg gttgcaaaaa gaggtatgga agtagaggca
     1981 gttcactttc atagttatcc atttacaagt gaaaggtcac aggaaaaagt taaggattta
     2041 gctaaaatac tggcaaaata ctgtggtaga gtaaggcttc ataaagttaa tatcttagaa
     2101 atacaaaaag caattggcga gaattgtaat gaagaagaat ctactattct ttcaagaaga
     2161 tttatgatga ggatagcgca gagactttct gaaaaaagac attgtgatgc tttaattaca
     2221 ggtgaaagta ttgggcaggt tgcatctcaa actatacaag gacttacttg tactaatgcc
     2281 gttgtagact taccagtgtt tagaccactt atagcaatgg ataaatcaga tatagttgat
     2341 atagccaaaa aaatagggac ttttgaaaca tctatagtgc ctgaagaaga ttgttgtagt
     2401 gtattttctc ctagaaagcc cgtcactaaa cctagattag agaaaataga aaaatctgaa
     2461 acagcacttg acattgaaaa attagtacag gatgctattg acaaaataga ggtagaggat
     2521 attgagtttt agcatacaag ttatttaatt actaaattga atattgagta tattagtagt
     2581 tctgtaagta tattagtagt tctgttgaaa ggatttatgt agacaattta ttaatatgta
     2641 gtatttaaaa ataagtacta taattttatt taatgggaat gattattatg aataaaaagg
     2701 gttttacact aattgaattg ttagtagtta tatctataat aggcatttta gttatagtag
     2761 ctgttccagc attgtttaga aatatagaaa aaagtaaagc agttacatgt ctttctaata
     2821 gagaaaatat aaagactcaa attgttattg caatggctga ggaatcaagt aaaggcaaga
     2881 atgaagtcat gaaagaggta ttagaaaaca aagatggtaa gtactttgaa acagaaccaa
     2941 agtgtaagtc aggtggaata tattcagcaa cgtttgatga tggttatgat ggaataactg
     3001 gaatagaaag cattgcaaaa gtgtatgtta cttgtacaaa acatccagat ggtgttgaaa
     3061 tggctaggga tatacatcaa agtatgaaag atttgattgc atcatttgca caagaccctt
     3121 ctataatacc aggagcctca aagggtaatg atgattttag aaaatattta ttagacaata
     3181 aatataaaaa tgggtggcct acaattccag atgaatttaa ggcaaaatat ggattaagta
     3241 aggatacact atatatacaa ccatatgcat ataatcctac taaatctgat gctactgtag
     3301 ttgtatttgc aaataataag actgggggta attggtatac ttccctagtt tacgattatg
     3361 atgaaggtag atggtataaa ggtaaaaatg gtatttctgt tgcaggtagg tcatgggatg
     3421 ttgacacaga tagtgttaag tctgtaaaaa cagagattca ttctaaagag ggatggggtc
     3481 ctttaaatta atatatcatg ttttattaat ctggtattga ttaaactata gaaaagagca
     3541 gtttctatag atgacgaata ccagattttc atttatatat ataattgaga tatattaaaa
     3601 gtgtttatat ttttaagtct tcacatacaa gctaaaaata attataataa tattgtaaag
     3661 aaatttaatt ataagattaa ttttctgaag atttagtatt tgtactaaga aatacataaa
     3721 aaggggaata gatatgaata ataattttca agacaaccaa acaataaatt taatacaaag
     3781 tcgtagaagt atacgaaaat ttacgacaga acaaattagt gatgagcaag taaacacatt
     3841 attacactgt gcatttgcag caccttcagg atgtaataaa cagccttggc acataactgt
     3901 tgtgcaagac cagaaacttt taaaagagat tagtgatgat actttatcta ggattcatga
     3961 ggtgagtaat gttgagataa ataaaaactt taagttattt tatggtgctc caaccgtttt
     4021 atttatttct tatgatgaaa gtagttcatg ggctccatat gatataggaa tacttacagg
     4081 gaatataaca acagctgcac aagcactagg gcttggaagt tgtataatag gaatggttag
     4141 aggtttattt acacctgttg aacaagggga tattgaaggt cttgtgagtg tattggataa
     4201 agaagatgta aaagaatcag agtcaataaa gatgaagttt gatacaaata aaaaatacag
     4261 agaactactt gatataccag aagggtattc tgtaccattt ggtattgctg taggtattcc
     4321 agatggaaat ttaccaaatg ctagagaggt agtttacaaa gtttccagag tttaggataa
     4381 agtattgcaa gaaccaatca gttacttgaa gaattaaatc agtaatatac aaataaattg
     4441 atggaagagg aagctattta aaaagtagtt tagatttttc gttgtaaaat aattaaataa
     4501 agaataaggt gataagtatt ttatttatta tcttattctt ttttattttt gttttgtaga
     4561 atgataaaaa taaggattaa gtgtaataaa atagaaaaca agtatgttaa ttttaagtat
     4621 atgctttaaa atttatccaa gttaaaaatt taagatatct tttcagtata atggaaaaaa
     4681 tgtcaaaata ttgaaatagg gaaaaaatag tgatataata agaaaaaata ga
//
LOCUS       DAMJZE010000233         4694 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJZE010000233 DAMJZE010000000
VERSION     DAMJZE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.237"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(154..957)
                     /gene="proC"
                     /locus_tag="PC541_003554"
     CDS             complement(154..957)
                     /gene="proC"
                     /locus_tag="PC541_003554"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2840974.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1282..3651)
                     /locus_tag="PC541_003555"
     CDS             complement(1282..3651)
                     /locus_tag="PC541_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2840975.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3656..4564)
                     /locus_tag="PC541_003556"
     CDS             complement(3656..4564)
                     /locus_tag="PC541_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2840976.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1383 a          908 c          512 g         1891 t
ORIGIN      
        1 aatatttaag taataaatta tcactcaatc attaaattcc tttttatagt aaattactct
       61 tattttactt ataccaaata catttatgta tagatataaa taagcactaa aaaaacaatt
      121 caagaaacca tatagtaaac ttaattacat ctgttatttg ctcatatctt tagacttatc
      181 tatacaagca acaatagatt ttataactgc tgctctaaag ccttcttctt ctaatacctt
      241 aacagcttct atagtagttc ctgctggaga acaaaccata tcttttaatt cacctggatg
      301 ttttccagtt tcaagaacca tcttagctga ccccattact gcttgagatg caaatttata
      361 agcttgttgt cttggcattc cagctaatac tgctgcatct gccatagctt ctataaacat
      421 aaatacatat gcaggagaag aaccactaat acctgtaact gtatccatta aatattctga
      481 tactatttct gcctcaccaa aagattcaaa cagagcctta atcatattta actcttcatc
      541 tgttacatta ctatttttac aaagagctga cattccttcg ccaacaaggg ctggagtatt
      601 aggcattact cttactattt ttttatctga acctataaaa ctttccagac tttctatagt
      661 tttacctgct gctatagata ctattatttt atttgcatct attaattctt taagtggagt
      721 taaagccttt tcgcatatat taggtttaag tgctactatt accacatcag aatttgtaac
      781 agctaccttt gaatcagttg tagtatttac tccaaatttt tcttttactt tatctaaggc
      841 aacttggctt aaatctgata cagttatatc ttctgccttt actaatcctg actgtactat
      901 gccacctatc attgcacttc ccatattccc tgaaccaata aatcctaaag ttttcatatc
      961 aaatctcctt ttttctatta gttttatttt aaagtagcta aattgacttt attttaactg
     1021 cttaaaatta tcttattttt ataaaatata accttattat aactataata aattattctt
     1081 ttgtatgtat tacttactaa tatgtgtatg atttactaaa tataaattta acgatttttt
     1141 cattaaactt aacgagctaa cttcctattt agaaagttag ctcataatta atctaaaatt
     1201 ttaaaaatgg aatcaaacca tttatacttt attatatcag ttacgatttt atatagtaag
     1261 aagttttatt tacaagcaaa cctagaatgt ttgctcagtt cttcctatta tctcatcttg
     1321 tagtgttcta cttaaattat tgaaatggtc actatatcct gcaactctaa ctattaagtc
     1381 tttataatcc tgaggattct tttgagcctc taacagtaca tttttatcaa atacattaaa
     1441 ttgtatatga tgaccatcca tattaaagta tgctcttact agattagcca tattatctaa
     1501 ccctttttct ccttgaacta ctgatggtgc aaatctttga tttaataatg ttccaccagt
     1561 ttttatgtgg tccatcttag cacaagattt tataactgct gtaggtccat tagtatctcc
     1621 acctttttca ggtgaaattc cctctgaaac tggttttgca caaagtctac catttggact
     1681 tgctcccata atttctccaa agtatatatg acaagttgtt ggtaacatat ctactctgta
     1741 ttctccacca caaacagttg gtctaccagt tacctcattg taatatgcat tgaatatctc
     1801 ttgcattatt tcatcagcat aatcatcatc atttccatat ttaggagtct tatttgaaac
     1861 taaattaagt atagcttcat atccttcaaa attagcatca agtgctttaa gcaatgtatc
     1921 catatcgaat tttttcttat caaatacatt atattttatt gctgttaaac tatctgttat
     1981 agttcctata ccaacacctt gtatatatct agtgttgtat cttgctccac ctctttggta
     2041 atccttgcct ttttctatac aatcatcaac tattgtagac ataagtggac aaggcatgtg
     2101 ttttgcacat atattttcta tcacagcatt tccttctatt tttatgtcaa tcatataatg
     2161 taattgcttt ttaaatgctt caaatagttc ctcataagat tggaaatttc tagggtctcc
     2221 agtttcaata cctatttgtt ttttactaat tgggtcatat ccattattta atgttaattc
     2281 aagtattttt ggaatgttca tatatccagt tagaacatat gcttctttac caaaacatcc
     2341 agtttcaaca caaccactgg ttccaccaag tcttgcatct tctatagttt ttccagcttc
     2401 cataagctct tgaactattg cttcagtatt ataaaatgct ggttgacccc atccttttct
     2461 tgatatttca caagctctct ttaagaattt ttgaggagtc tttttactta tttgaacatt
     2521 tgaactaggt tgtatcaatt tcatttcatc cataacatca agtattatat aactaacttc
     2581 attaacacca tcttgaccat ctggatttat tccaccagtg tttatatttg caaaatctgt
     2641 atatgtgcta ctttctttta atgttatacc aacttttggt ggtgctggct gattgttaaa
     2701 tttaacccat aaacattcaa gtaattcttg tgctctatct ctatctagta ttccattttc
     2761 tacatctctt tcatagaaag gatttagatg ttggtcaagt cttcctggag taaaagcatc
     2821 ccatatatta agttcagtag taacccctat atgaacaaac caatacattt gtatagcttg
     2881 atgataagtt tctggtttgt gagctggtac aacatcacaa gtttcagcaa ttagtaataa
     2941 atcttttttc ttagcttcat ctgtttcttc ttctgccatt tgtcttgctt tctcagcata
     3001 tcttttacct aatataacca ttgcatcaca agctattgcc atagcttcta aatctgcttt
     3061 tttattatat gcatctaaat cattaagaaa atctagctct tttaatcttg cttctatgtc
     3121 tttctttaaa tctaaaaatc cttttttata tattgtatct ccacaaactg tatgtcctgg
     3181 agctctttgt tccataaact ctgtaaacat acctgcttca tatgctgcta accactcagg
     3241 tgtcattgca tttattatct tatctcttgt ttgtctcttt ttccaaaaag gaattatttc
     3301 ttctttatgt atttttctag cttcttctga tacgctaaaa tttattatat ctctattgtg
     3361 cattacttct aaatcttcca ttgtatgaca acaaatttct ggatatgttg gtgctccatt
     3421 aggagaatca cctttttcac ctactattaa ttctccatca tttatgctaa gagttctatt
     3481 ctcaatataa tgcttaaatg ataaagcacg aagtacagga atctctacac ttccctcata
     3541 ttttttataa gcttctgtca taagtactgc tctttcaata gatatgtgtg gttctgcatt
     3601 aatactttct tctcttaatt ttttagttct ctcaaaagtt cctcttgcca ttttattacc
     3661 ctccaatctt agtattgaaa ttacttgctt caaacagtct ttttatttcc tctaattttt
     3721 cttctgatgg tctttgtagt tcttctcctt catacttttt ataaatttta tcatacttgt
     3781 gtttaccaat attatgatat ggaagaagac ttactgcttg tatattcaaa ggctttaaaa
     3841 actctatcat ttttttaacc tctaaatttt catcatctac atttactcct acaattaatg
     3901 gtattcttat atttatgtta gcccctaatt cacttaaaat cttaacattt ttcagtatta
     3961 agtcatttga ttttcctgta aattttatgt gcttatcttc atcaataagc ttaatatcat
     4021 ataaaaacaa atctgcacat ttagcaactc tttcataatt ttctgacttt gcatagccac
     4081 atgtatcaat tgctacatgg attccctttg atttgcacat gtttattact ccacaaatga
     4141 agtccatatc ttgagccatt acttctccac ctgatagagt tactccacca cctgattctt
     4201 catagaaaat tctatccttc tctatctctt taaccaaatc tcttacagaa tactcttgac
     4261 caacaatctc tcttgcattg tttacacaat agtctaagca agtttcacaa aactcacact
     4321 tatcttggtc taagcatatt tttgtttcac ctttgtaaat tgccttatga gggcatatat
     4381 ttatacatgc ttcacatttt gaacatcttt cttcattata caaaacttgt tttgtatatg
     4441 tttgactctc tggattatga caccaaacac attcaagtgg acacccttta aaaaatactg
     4501 tacttcttat ccctggtcca tcatgtatgc tacatttttg taagtttata actaatggat
     4561 tcatgatttt tcacccctaa ataatatcca ttccattttt tcatttttat gcaattcatc
     4621 ttcatagaat atacctatga aaagcatttc tacaattaca taatactata ttcgttatta
     4681 ttttgtctat atat
//
LOCUS       DAMJZE010000234         4542 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJZE010000234 DAMJZE010000000
VERSION     DAMJZE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.238"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(247..1383)
                     /locus_tag="PC541_003557"
     CDS             complement(247..1383)
                     /locus_tag="PC541_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2840977.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(1415..1960)
                     /locus_tag="PC541_003558"
     CDS             complement(1415..1960)
                     /locus_tag="PC541_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2840978.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            2284..3054
                     /locus_tag="PC541_003559"
     CDS             2284..3054
                     /locus_tag="PC541_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840979.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            complement(3508..3936)
                     /locus_tag="PC541_003560"
     CDS             complement(3508..3936)
                     /locus_tag="PC541_003560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2840980.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(4132..4365)
                     /locus_tag="PC541_003561"
     CDS             complement(4132..4365)
                     /locus_tag="PC541_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2840981.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
BASE COUNT         1576 a          677 c          479 g         1810 t
ORIGIN      
        1 attataatga caaataatag gaatattttt tgaataatta atagcaaaaa aactaatcaa
       61 ctgaaacatt atatttttag aatatattaa aattgggata ttttcaccta aagaaatttg
      121 ctttcctagt tcctcactat aactcaatat attttcataa gtatattttt ctccatctat
      181 aattaaaaaa tttttatttt tatgcctctc tttttttatt cttaaaagtt cataatagtt
      241 cataatctac accctttcaa gcagtaaagc tgaacctaga ccaccagctg cagctataga
      301 gcaaattcca tatctaccct tagtcttttc taatgctttt aaaagatgta atgcaataat
      361 tgcacctgaa gcaccatatg gatgtccata cgctaaagct ccaccaaata tattatatct
      421 atctattaac tctggatact ttctttgaaa caacacatct ataactgcaa atgcttcatt
      481 aaattcaaca gcagatacat ctaaataatt aaggctttct atctctaaaa gtttatccat
      541 tgcttgtatc gctgatgttg gacttaaggt tgcatctgtt cctatcgtgc atgtgttaat
      601 aatttttgct ttaggcttct tttttacatg ttctaaatac ttcttagaac atattataat
      661 aaatgaagct ccatcattaa taagacatga atttgctgca tttgtaatag tttcttttcc
      721 taaaattgat ggcatcctat ctaataacct ttgactcatc ttatcacgta taccttcatc
      781 ataggtacta ttatttatag gagatataat atcttcaagt attttttcct ctcttgcttc
      841 ttttgctctt ttatgacttt cctttaccca aaaatccaag tctgctttct caatttgata
      901 taactctgct actctttccg caccttcgag catactattt tggctattat catcaggaga
      961 aaattgagca actgtataat ttggattatt tgtattatat cgcttatcat tcttatgata
     1021 tgtgcgcatt ggttgcagag aactactttc aaaaccacct gcaataatta aatcacactg
     1081 accagattta acctttgaaa aagctatgtc tatactcatc atagcagaag cacactgcat
     1141 atccacagta aaagcaggta cttcatttga cactcctgct gttaaagtca ttaatctagc
     1201 tatattccct cctgtcccaa ctgcatttcc acatataatc tcatcaattt tgtcaatttc
     1261 atacttttta attaaatctt ttaaaacact tgctcctagt aattcaggtt gaacaaattg
     1321 aaaaattcca ttttttagac ctatatgact tctcaatcct cctaatataa aaacttcctt
     1381 catacattac acccttcctt atattataga tttatcatag atttcttctg agtacattgt
     1441 tcagtttagt gcctaaaaaa gatgcaataa ctgactttat tgtatctcca attaaaaatg
     1501 gtactactgc tgcaaaaagt gctgcttcta ctgtcatttg attaaaatag cacataaata
     1561 ctgttcccat aaaatatata ataggcattc caacaaaaat tgttattaat atatatctct
     1621 taatatcatt atttcgtcct tttaataaac taataattag tgctgcaaac aaaaacccaa
     1681 aataaaaacc acctgttggg cttaataact ttccaatacc agatgtacct ccaccaaata
     1741 ctggaatccc aattaaacct atcattatat atacaccaac tgtactaaat gcttgtcttg
     1801 gtgttaaaat caaagctatt aaatttatca ttattgtttg tgctgttact cctgctggag
     1861 taaatggcaa tggtatatat atgtaagatg atatacacaa taatgctaca catattgaca
     1921 ttttagttaa atcttgaata tttactttag tatttttcat ttttataatt cccttcatat
     1981 gtcttatttc atttagttta tatgtacata tacaaagctt ctattaaagc tttgttttag
     2041 tataaaataa agacgttttt ttgtcaacta ttataattat ttaggtttac atttaattta
     2101 taagtaaact ttatactgtt actttatatt ttaatagtca tttataaaac tttgcttaaa
     2161 aacagctcta tttagactat acttgtaaat tataatatga tatattttta tataagattt
     2221 tattttatat aaaaaatagt aacaaatagt ttatacacaa gatatttaaa aggaagtgat
     2281 gatatgaaag ttaatgaaaa taatatagat gaagagttag atgaacttag taggaaagtt
     2341 tacttagagt caccagattt ttggataagt atttctgaca aaataataga taaagtattt
     2401 gatgaaatgt tattattttt tgcagttaaa tataactatt tcaatatagt atgctttgct
     2461 attgaaaaca attatctaga tttaaacatg ccttcaaaaa acaaaaaata cactaatata
     2521 atggagcatt tgttagatac atcaaaacag aatgaagata aaaaaattta taattatctt
     2581 ctaagcttaa ataaaggtaa taacctagat gaaagaccaa ttttagatga attaaactgt
     2641 aatattagta gtgatacttt tgaaaaaaaa gatatatatc ttccaaagta cttatgtcca
     2701 agttgtaatt ctaatatatt tgatacaggt tataaagtat caaatgacat aacatataaa
     2761 ttttcttcta aagaagctga acctattgaa atgtgtagtg aagtttcttc tatcagatgt
     2821 tgtaattgtg atgaattatt agaagatatt acacttgata aactatataa cctatgcaaa
     2881 attcaaaact gtaataattg tggttctaat ttaactaagg taggaattat tgcaaagaaa
     2941 aaaatgaact ttgatgagaa tactgaaaaa tttaaagatg tctcaacatt ttactgttgt
     3001 ggaaattgtg actctgaaat aagcttatct caaagagaac attttaagat ttaaatattt
     3061 ataggctaaa gtaataagat tacattatta gatactgtat aagtttttta aatctgtaaa
     3121 aataaataca cttataagaa ttaaaattgc tatgaaacta gttcttgatg aactaatttc
     3181 atggcaattt tctataatct aataattatt aaattttaac aaacctttat tagcattcca
     3241 taattattta aaattctttc aatactctat agtttatagg tgaaagtatc tttaatatta
     3301 tatcaaactt attatcttta aatagtaaat ttgttaaatc tgaaatacac catacaattt
     3361 tattttcctc ttttgtagct atatcaattc tgattaactt acatctataa taaatatctt
     3421 tatcttcata tagtctattt taaaaattac tcttaaaaaa tcaactagaa aataaattaa
     3481 ttatcctttt aatagaatgt aatcatatta tttactatat tccattatgt actctttaaa
     3541 tcctgaatct tcatttactt gttcttttac tatattaaaa ccttttttat tataaaacac
     3601 aactgctttt ttattatctt tatatactgc taagcttaga cttttatatt ttttagttgc
     3661 ataatctaaa agtttacctc cgatacctag attttggtat ttgggcttta taaataaagc
     3721 tcctataaag tttttatcta ttatgcttat aaatcctttt atttcatctc catcatcata
     3781 tacaaatgta tctgatatag gtatatattc gtttttaaca gtattatagt tattttgcca
     3841 gtattcttta cttataaagt catgtgcttt gattgtactt ttttcccata tttccatgat
     3901 tttatttatg tctttactgt ttaatttctt tatcataaat tttgtttcac ctcatttttg
     3961 tattgatttt ataatgtttg taggtaagtg tattttattt agatatgatt tagttaatat
     4021 aaggtggatg ttttagtata tgctattgtt gattctacaa ttttatatac tgctttatta
     4081 atataaaata agtttctaaa tactcgttga tattgctaat attctactta tctaccactt
     4141 agttgatata aaaattgaaa cttcactatc taataattta attaatgata tgctattaca
     4201 tttatctatt tcataaccac cataaatccc aggtgttgaa tttataaata taccagcttg
     4261 ctctaaatac atcttgtaac tttttatttg cctttcactt acttccaact cttctgataa
     4321 ttctttgcat ttcattttgc ctctgctttg aattaatatc atcattctaa gtgtattagc
     4381 taatttacta atgagtaacc cccccctaat aacattaatt gatattttta ttatttattt
     4441 ctacacatat gtacatataa cctctttata ttttatataa gtagaatttt tttataattt
     4501 tgacatttac catattatat tttatatttg tacaaaattt ta
//
LOCUS       DAMJZE010000235         4463 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJZE010000235 DAMJZE010000000
VERSION     DAMJZE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.239"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     misc_binding    75..309
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            359..1378
                     /gene="pheS"
                     /locus_tag="PC541_003562"
     CDS             359..1378
                     /gene="pheS"
                     /locus_tag="PC541_003562"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2840982.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1395..3788
                     /locus_tag="PC541_003563"
     CDS             1395..3788
                     /locus_tag="PC541_003563"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2840983.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3813..4403
                     /gene="zapA"
                     /locus_tag="PC541_003564"
     CDS             3813..4403
                     /gene="zapA"
                     /locus_tag="PC541_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2840984.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1761 a          520 c          917 g         1265 t
ORIGIN      
        1 attggatata tattaaaaat agtcacataa tttgaatgga aatgatataa tactaaaata
       61 aacaatataa tattgtaaat gcaatgaaag aggaaagtat tttgattaaa cttagtaaag
      121 agataaacac ctaggctggg agtgttttct aagaggtcat gagaagttcc ctctggagta
      181 acagagctga aattttacag taggctttga cgtcaaaaac gcgttaagtt tgttagaggt
      241 ggtttgatga tttttaaatt gttaaactac tagggtggta ccgcgaaact atagcctttc
      301 gtccctagac agggattgag gggctttttt tatacaaaaa aacgaaaggg tgatatgtgt
      361 gcaagaaaaa ttacttgctt tacgtgaagc agctttggct gaaataaaag aagcacaaag
      421 catagaaagt gtagaaagtt taagagttaa gtacttagga aaaaaaggtg agataactgc
      481 catacttaaa gaaatgggta aattatctgc tgaagaaaga ccagtagttg gtaaggttgc
      541 caatgaggta agagaaaaca ttgaacttag cataaattct aaaaaagaag aaataaatgc
      601 tattgaaaaa gaaagaaaat taaaagagga agtgatagat gttactcaac caggaaaagt
      661 tttaaaggtt gggaagaagc atccaataac tcaaattata gatgaagtaa cagatatatt
      721 tatcggaatg ggattctcta tagcagaagg gccagaagtt gagactgttg aaaacaactt
      781 tgacgcatta aacgctccta aagaccatcc atcaagagat atgagcgata cattctatat
      841 caatgatggg gtattactta gaactcaaac atctccagtt caagtaagaa ctatgagaag
      901 tcaagagtta ccaataaaag taattgcacc aggtagatgt tttaggtcag actcgccaga
      961 tgctacacac tcaccaatgt tccatcagat agaagggctt gttgttggaa aagatgttac
     1021 tatggcagaa tttaaaggaa ctatggatat cttcgttgaa aaattgtttg gttctgatat
     1081 caaaactaag tttagacctc acaacttccc atttacagaa ccaagtgcag aggttgatgt
     1141 tacttgtttc aaatgtggtg gtaaaggttg cccaatgtgt aaatatgaag gttggataga
     1201 aatattaggt tcaggtatgg ttcatccaaa tgtgcttaga aattgtggaa tagacccaga
     1261 agtttacagt ggatttgcat ttggagttgg ggttgaaaga cttgcaatgc ttaaatacga
     1321 aatagatgat attagattat tattcgaaaa tgatatgaga ttcttaaatc aattttaatt
     1381 aggagggtat gtagatgtta gtatctttaa aatggcttag agactatgtt gatatagaca
     1441 tggatgtaaa agagttcgct gataaaatga caatgacagg aactaaagtt gaaacaatag
     1501 attattatgg tgaagaaata gaaaatatat tggttggaaa gattttagaa ataaaacaac
     1561 atccaaatgc tgataagttg gttgtaacta aagtagatat tggagataaa gttgttcaaa
     1621 tagttacagg agctacaaat atatcagaag gagattatat tccagtagct gtaaatggtt
     1681 ctaagttacc tggaggagtt gaaatcaaac agactgattt cagaggtgaa ttatcagatg
     1741 gtatgatgtg ttcagcagct gaactaggta tagatgaaca ttacattgag gagtataaaa
     1801 gaggtggtat atatatttta gaccacgaag attcttatga attaggaaaa gatataaaag
     1861 atgttttagg attaaaagat gctttaatag attttgaatt aacttcaaac agacctgatt
     1921 gtaaatgcat gatgggtata gctagagaag cagctgcaac tataggaaca aaagtaaaat
     1981 atcctgaaat cgaagtaaaa gaaagtgacg aagagataga tttcaaagtt gagatagata
     2041 atccagattt atgtagaaga tatgttgcta gaatggttac agatgtaaaa atagaacctt
     2101 ctccatattg gatgcaaaga agacttacag aagcaggagt aagacctata agtaacatag
     2161 tcgatataac aaacttcgta atgttagagc ttggtcaacc acttcatgct tttgatataa
     2221 atcaagtaga gactggaaga atagtagtaa gaaatgctaa agatggagag aaacttgtaa
     2281 cattagatga tgttgagaga acattagaca aagatatgct agttataaca aatggagaaa
     2341 aatcacttgg tttagctggt gtaatgggtg gtgctaactc agaaataact tctaatacga
     2401 agactgtact ttttgaaagt gccaatttca aaccagaaaa cataagaatg acagctaaaa
     2461 aagttggtat taggtcagaa gcatcttcaa gaaatgaaaa agacttagac cctaatcttg
     2521 cagagatagc agcaaataga gctgcacaac ttgttgaaat gttaggagca ggaaaagttt
     2581 taaaaggtgt tgtagatgta tatccaaata aaccagaacc taaaaaattg gtagtaaatc
     2641 ctcaaagaat taaccaccta ttaggtgtag atgtaccaat ggagcagttt gtaggaattt
     2701 tagaatcatt agagtttaaa tgtaatttgg tagctaatga taaattagaa atagatgtac
     2761 caagctttag aacagatatg gaacaagaag ctgatgtatg ggaagaaata gctagaattt
     2821 atggatttga gaatatacct tctgtacaat tagaaggaaa tacgacagca ggaattaaaa
     2881 cttcaaaaca aaaattcatg gatgctttaa aagataattc aactgctgta ggattaaatg
     2941 aaatattaac atattcattt gtaagcccta agggagtaga taagataaga gtaccagaag
     3001 gtaatgcaaa gagaaatttt gttaaattac ttaacccatt aggagaagaa acttctgtaa
     3061 tgagaactac tttaatacca aatatgttag atgttttatc aactaatgta tctcataaaa
     3121 tagaagaagt atctgcattt gagtgtggac atatattcat cccacaagat tcagaattac
     3181 ctaaggaaga aaatagaatg tgtgtaggta tgtatggtaa agatgttgac ttctttgcac
     3241 taaaaggaac tatagaaact atacttgtaa atgtcggatt taaaggttat gaaattgaac
     3301 ctcaagataa taatactacc ttccaccctg gtagatgtgc taaaatagtg tataataata
     3361 aatatgtagg tacattaggt gaattgcatc cagatgttat agaaaactac aatctaggtc
     3421 aaagagttta tgtcgctgaa atagatattg actttgtatt tgataattct gatagaacaa
     3481 agaattatgt gccattacca aaatatccat ctacatcaag agatatagct cttattgtta
     3541 aagatgatgt atttgtaaaa caaatagaag atataataaa ggaaaatggt caaggattag
     3601 tagaaagcta caagttattt gatgtttaca aaggttctca aatagaagct ggatacaagt
     3661 caattgcata ttcaataact tatagaagta aagataaaac tctaacagat gaagatgtag
     3721 ctaaggtgca tgataagata ttaagtgaat taagcgaaaa attaaatgct aacttaagat
     3781 caaattaaca actaatgtcg ggggaggtta ggatgaacaa agtaatggtt aaaatccatg
     3841 gcgctgaata tccaatggtt ggagacaaat cagagaagtt tatgattagt attgctgatt
     3901 ttgtagacaa agaaatggat aaaataacac gtcaaaaccc gaaattgagc ttatcagttg
     3961 ctgcgatatt aactgcatta aatatatcag accttttatt tgaatgttca gatgagaatg
     4021 agaagttgat taaagcgaat gaagaattga gtaagaaggt tggagcatct aatgaagagc
     4081 ttcaattaga aatcaaatct ttaaagctta cgatagcaga aaaagaagca gaaaatcgtg
     4141 aagctgaaac taagatgaaa gagttaattg agattataga aaataaaaag caagaaatat
     4201 tcgaacttag taatacgaca gaaggttcta gagcagagtt agatgcttat aaaaacaaga
     4261 ttgaggagtt aagtactcaa cttgaagaag caaatgaaag agctactata gctgaaaact
     4321 tagcttctga gtttcaaaac aaagcttatg atttacagtt aaaatgtaca gggttaaata
     4381 atgatgcaaa aaatgtggaa taaactaaaa gatttgctgt ctcttgtttt ttgagacagc
     4441 ttcttttaat gtataaatat aaa
//
LOCUS       DAMJZE010000236         4455 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJZE010000236 DAMJZE010000000
VERSION     DAMJZE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.240"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            163..1302
                     /locus_tag="PC541_003565"
     CDS             163..1302
                     /locus_tag="PC541_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2840985.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1328..1516
                     /locus_tag="PC541_003566"
     CDS             1328..1516
                     /locus_tag="PC541_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2840986.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2300..4084
                     /locus_tag="PC541_003567"
                     /pseudo
     CDS             2300..4084
                     /locus_tag="PC541_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1915 a          490 c          721 g         1329 t
ORIGIN      
        1 gtaacatata ttttaaatgc ttattcttaa atttttgcat tatttattaa aatttattat
       61 gctttaacgc taaattgagt ttgataatta taaaacaaat aatcaaaaat agtgtaaaaa
      121 ttaatacttt ttgttaatat taatagataa agataatcta aggtggtgat taaactgaca
      181 atagaagaat acaaaaacaa atactttact ggaaataaaa tcgtactttc tataaatagt
      241 tttgataata aagaactata ctatcatgaa actgaaaata tattaaattt aataaatgaa
      301 atagataaat taacatcaag taagatattt aaaacaatag aagataaaaa tgatttggca
      361 ctagagtctt attattctag tgtaatagaa ggtgcttttt caactagaaa gattgcaaaa
      421 tctataatta gaggaaagat gaaaccaagt aataaaagtg aatatatgat ttacaataat
      481 catagagcct tagaatatgg attagataac ttagatgaat tgtatagtca caaatttata
      541 tatgatttgc atcatatatt gggcgaaaac tgcttggatt cagaagaata tgagtataga
      601 actgaaaaag tatatgtatg tgattcaaaa ggtgagatta tacatacagg gcttgaacct
      661 ttgaagatat atgactttat tagtaaatta atagatttta tggaaaatag caaagtaagt
      721 aatttaatta aaagtgctat aattcatttt tactttgtat atgttcatcc ttttagtgat
      781 ggtaatggaa gaacatcaag agccttatca tatctatatt tgattgataa aggatatgat
      841 actttcaagg atttttcaat atcatatatg atatctaaaa acagaactaa gtattataag
      901 gctatacttg atgtagaaaa caaaggaaat aatttaactg tatttataga atttatgctc
      961 aagtctataa tacaatctat taatgagatg cgtaatatgc atgatagaaa atctttagaa
     1021 agtatattaa aagaagaatt gtttgaaaat gacataactc tatcagcaac agaagaacat
     1081 atattaaaat atatttgcaa taaagataac tattctatga cattagaaaa ttatataaaa
     1141 aagaataaaa gtagatactt aaaggctgga ataaaggaaa ttgaattagt tgaccagttg
     1201 atggaagtgt ttaataatct tgaagagata gagattttgt ctaaagaaaa aaatatatat
     1261 aaagttaatg ataagtactt aaaaatgctt gatatagact aatttagata atctggagag
     1321 ggaatatatg aataataaga aaaaggcatt tttgatttct ggggctataa acgtaatttc
     1381 attaattatt gttttttcat tgtttcatta taatattata gaaaatagta cacaaaatat
     1441 gataataact gtaatcttaa tttttatttt tgaaatgata aaaataaaaa tcatagagaa
     1501 atactataaa ctataaacaa gaaatatctt ttctaagagc atattgtaca atatgctctt
     1561 aatttagtta tatagattta atataaataa tgtatttttt gtagatgatt tatgtaagat
     1621 ttatgaccaa tttgtgtatg atttgtgttc tttaatcatg acaatattat attgtaaaaa
     1681 ataaatttaa taaaaaatga cttggaattt atattctagg tcttttttat ataaaaattt
     1741 aataagtttc aaatgttggt aactttgaca gtagaaaacg tctattgaga taacgaggat
     1801 tctaattgag gtggagagat attagcactt aaagtagtat atgcaacaga tataatcttt
     1861 acaaaagtaa tttgaattaa aatgtaaata taaagaataa ttaaaaaaat atcgcaatta
     1921 tttgtttttc atatggataa aattggttat ttaatatata attacaagta taaataataa
     1981 aatttatttg taaaaagagt agcactttta attctaaagg ctactttttt tatacaaaac
     2041 cctatttttt ataacaattc attaacataa aaatcaaact taccttaaaa tataaattta
     2101 caaatttttc aatcaataag aaaatttctt tattccttac ataatgactg caatgtaaca
     2161 aaataattac aaattaatta tgaaatcatt atagttagaa acctctttaa aattagatat
     2221 taagtatcct ttagaattag aacgggaaca aagttaaaaa attaagtaat atcaaatgga
     2281 caagggggaa gaaaataaaa tgaaaatacc aaagaagatt gctgcaatgc ttacagttac
     2341 aatgatagct ggaagttcaa cagcaggcat agctagtgca caaactgtgg caacaaattt
     2401 aacagggcaa gaaagatatg aaactgctgt taaaataagt caagatggat ggaaaaatgc
     2461 tgatgaggta gtaatcgtaa acgactcatc tatagcagat gctctatctg caacaccatt
     2521 tgcaaaagca aaaaatgctc caatactttt aacaagtaaa gataaattaa atgataagac
     2581 gaaagcagaa atacaaagat taaaagctaa aaaagtatac ttaataggtg gaacttctgt
     2641 attaagtaca aatatagaaa aagaaataaa agatttaaaa atatcttttg agagaatctc
     2701 tggagctgag agatatcaaa catccttaga attagcaaag aaattagatg ctataagtga
     2761 tgttaagaaa atagctgtag ttaatggtga aaaaggactt gcagatgctg taagtgtagg
     2821 agcaccagct gcacaaaaca atatgccagt aatacttgct gattctaaaa atggaacagc
     2881 agttgcagac aaatttataa aggatgctgg aataactcaa tcttatgtag taggtggaga
     2941 gagttcaatt tctgaagctg ttaaaaataa actaccaaat agtacaagac taggtggaac
     3001 agatagaaat gatacaaatg caaaagtaat aaaagaattt tataaaaaga cagatttaaa
     3061 aaatgcttat gtaactaaag atggtatgaa taaacaagac caacttatag atgcactagc
     3121 agtaggtgta ttaggtgcta aaaatcaatc accagtagtt ttagttggaa agaacttatc
     3181 agcttcacaa aaatctctag ttaactctaa gagttttgat aagataacta aagttggtgg
     3241 gaatggtaat gagacagcat ttaatgaaat gaagtcatta caagaagtaa aaacagttga
     3301 agctaaaaca atttctgaat taaaatcagc tatagataaa gctactgcta atgatgttat
     3361 aaactttaag ccaactagtg aggttaaaga agcatttaca atacaaactg ataaagcagt
     3421 aacagtaaac ttaaatggta catatacaaa gactgtgact atcaacatgc ctaatggtga
     3481 tgtaaataac tatgcaaaag tggatgatgt agttatagat gatgtaaaag atggaacttt
     3541 tgttaattat ggaaaaataa ctaacttaaa ggttaatgat aaaaatggag ccaagataga
     3601 aaataattct aaaggtgaga ttggtagttt aacagtagca tctggagcaa gtcaagtaaa
     3661 agtgactaat ggtggtaaga taactacagt tacaaataat tctaaaggga ctactataga
     3721 taataaaggt acaataagta gtgttaaggg agataattct ccaacaataa gtggaaatag
     3781 tccaagttct aactcttcag gaggaagttc atcaagtgga ggttcttcac atggaggagg
     3841 ctcatcttca ggaggaagtt cttctaatca aacatcagta aataatgaag cagcaaaaat
     3901 aacaagcgtg cctacaccag caaaagatgc aactagatta actatgccat cagtatcaag
     3961 tggatatact atagcaataa aaacatcaag taatgagagt gttataaaaa aagatggaac
     4021 aataatacct ccaaatacag caacaacagt aaaattagta tttacggtaa cacatacatc
     4081 aagtggtaaa acagcagata caaaagaatt atctgtaaca gtaccagcta aatcaacaga
     4141 tgaagaatta caagcagcat tagacaatga agtggcaaaa ataacaagcg ttcctgcacc
     4201 agcaaaagat gcaactaaat taactatgcc atcagtatca agtggatata ctatagcaat
     4261 aaaaacatca agcaataaga gtgttataaa ggaagatgga acaataatac ctccaaatac
     4321 agaggaaaca gtaacattgg tatttacagt aacacaagaa tcaagtggta aaacagcaga
     4381 tacaggagaa atagatgtag tagtaccagc caaatcaaca gatggggaaa tacaagcaga
     4441 agtacaagct gaggc
//
LOCUS       DAMJZE010000237         4381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJZE010000237 DAMJZE010000000
VERSION     DAMJZE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.241"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            141..1616
                     /locus_tag="PC541_003568"
     CDS             141..1616
                     /locus_tag="PC541_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2840987.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1703..2116
                     /gene="speD"
                     /locus_tag="PC541_003569"
     CDS             1703..2116
                     /gene="speD"
                     /locus_tag="PC541_003569"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2840988.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2146..2997
                     /gene="speE"
                     /locus_tag="PC541_003570"
     CDS             2146..2997
                     /gene="speE"
                     /locus_tag="PC541_003570"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2840989.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2987..3865
                     /gene="speB"
                     /locus_tag="PC541_003571"
     CDS             2987..3865
                     /gene="speB"
                     /locus_tag="PC541_003571"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2840990.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4045..4245)
                     /locus_tag="PC541_003572"
     CDS             complement(4045..4245)
                     /locus_tag="PC541_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2840991.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1732 a          494 c          750 g         1405 t
ORIGIN      
        1 tcaaaaaaac acttgtaaaa atgctgagat gttaatataa taaatatcat gagtttaata
       61 aaagtaaaaa taaaaaatag aaataataaa taataaatat taaaaatatt tgaatataga
      121 gaggagatga atttgaaatc atggatagcg agtttgcaaa tgcagttgaa ttaagctatg
      181 agtgtgctcc attattagag tcgttaaagg aatactcaga gaaagatata gcttgttttg
      241 atgtgcctgg acatgtaaaa aatagaggtg tagctatatt aaataaatat ctaggtgaaa
      301 gtctcatgaa aatggatata aattcatcac caactatgga taatgtatct gcgccaaatg
      361 ggattataaa aaatgcacaa gatttgctag cacaagcata tatggctgat gaggcattct
      421 ttataacgaa tggcacaact caagcaatac atgcaatgat tttgagtgtt ataaaaccag
      481 gtgaaaaagt attacttcca agaaatatac ataaatcagt aataaatgcg ttaatacttt
      541 gtggaggaat accaatattt atacaaccag aatttgatga aaaactaggt ataagtttaa
      601 atattactct tgaaaaagtt agaacagaga tagaaaaaga ttgtaatata aaagcattat
      661 ttttccttaa tccaacttac tatggagttt gtgcagacct tgagagtatt atagaactat
      721 gccataaaaa taatgtttta gtgttagttg atgaagctca tggtgcacat tttccatttc
      781 atttagattt acctccttct gcaataagtt tgggagctga tatggtagct gtaagtatac
      841 ataaaactgg aggagcattg acacaatctt cagcactctt attgaataga gataatgtaa
      901 gttttgaaaa ggttcttcaa tcaatcaata tgctacaatc aacatcagca tcttatttgc
      961 ttatggcaag tatagatggt gctagagtta atctagttga gaatggtgaa aagcaactgt
     1021 caaaagcatt aaatctatct aggtatgcaa aatctaaatt aaacaaaatt gatggaataa
     1081 aagttttatc tacagaaata ttgaaacaaa aaggtgttaa atttatagat gaaacaaaat
     1141 tgtgtataaa tgtaaaagaa ttaaatttga ctggatttga agtatacgat ttattatata
     1201 aaaatttttc aattcaagta gaattgggag attcatataa tatactggca cttgtgtcca
     1261 ttggaacaaa taaatcagat atagatagat tagtaaaagc actttctata attgcaaaga
     1321 tttatagaaa agaatcaaca ttaaatgaat ttaatatggt tcaaataaat ccaattatta
     1381 aacttaatcc aagagaagct ttttatgcac caaaggaaag tgtagaaatc aattcatgta
     1441 tagatagaat ttgtggagaa tcaataatgg catatccacc aggtataccg attattgctc
     1501 caggagaatt gataacagaa gaaattatgg agtatattat tttcttaaaa aatagcaatg
     1561 catatcttac agatgtacaa gataaaaact tagatagaat attagtaata aaataaatta
     1621 ctttattttg caagggcaag tatttgttaa gtagagttta tcaaatagaa cttattaaaa
     1681 gtataaagga gttggggttg aaatgaaatt tgaaacacta ggaagacata tactagtaga
     1741 atactataat tgtgatgaaa acgtacttaa agacccacgt cttatagaaa attttatgaa
     1801 tgattcagca ttaaatgcaa aagcgacaat tgtggattct gtattccacc attttaatcc
     1861 ttggggggtt tcaggagctg ttataattgc tgaatctcat ttgacaatac atacatggcc
     1921 tgaatatggc tatgcagcag cagatttttt tacttgtggt gatatagacc catggaaaag
     1981 ttttgaatta ttagagcaac ttttaaagtc tgaaagaagc gagtctacag aaattccaag
     2041 aggcttaact acaaaaatta aaaaatattc aaaaaaagat ttaggtaaga ttactcataa
     2101 accagaagca gtataaaaca tacaagagaa agtgaggatt attatatgga actttggtat
     2161 acagaagaat ggacagaaaa tgtaagattt tctataaaag taaataaaca tctatttgaa
     2221 ggaaagtctc aatttcaaag gatagatgtg tttgatagtg atgaatttgg aaaatttcta
     2281 acaatagatg gattaatgat ggtaacatat aaagatgaat ttatatacca tgagatgata
     2341 actcatgttc ctatggctac aaatttaaac ataaagaaag tattagtcat aggtggaggt
     2401 gatggaggta cagtaagaga attaagccgt tatcctcaaa tagaaaagat tgacatggtt
     2461 gaaatagata aaatggttgt agatgtatct aaggagtata tggatatatg ctcatgtaaa
     2521 ttagatgata aaagagtgag tttatatttt gaagatggtg ttaattttgt taagtgtgca
     2581 catgataaat catatgacct gataatagta gattcaactg acccaatagg accaggtgaa
     2641 gggcttttct caactgattt ttataaggat tgttatagaa tactgactga tgatggaatt
     2701 ttagtaaatc aaagtgaaag tccgtatttt gactttaatg ctaaagagat gaaaagagct
     2761 aataaaaaat taaaacagat atttccaata tcagaagttt atcaagcaca tataccaaca
     2821 tatccatctg gtcactggtt gtttggattt gcttcaaaaa aacttaatcc agtaaaaaat
     2881 caagatagaa atggatggga gaaattaagt ttaaaaacta aatattataa tagtgatata
     2941 catttaggtt cgtttatgtt acctcaatac gtgaaggaga tgctagatga agaataattt
     3001 ctatcatatg aacactttta tgagtatgga caaaaattat gaagaatcta atcttatagt
     3061 atttggtgtt ggatttgatg gaactacttc taacagacct ggggctagat ttgcaagtag
     3121 ctctatgaga aaagaatttt acggtcttga gacatatagt ccttttttag atttggattt
     3181 agaggattat aatatatgtg attatggaga tttagaaatt agtgttggaa gtacagaaca
     3241 agtcttaaaa gaaatctatc aagagacata taagattgtt agagattcaa aggtaccctt
     3301 tatgattgga ggagagcatt tagttacatt accagccttt aaagcagtac atgaaaagta
     3361 caatgatata tatgtaattc attttgatgc ccatactgat ttgagggaag aatataataa
     3421 tagtaaaaat tctcatgcaa cagtaattaa aagaatatgg gatattgtag gtgataataa
     3481 aatatttcaa tttggtataa ggtctgggac aaaagaagaa tttaaatttg ctacagaaga
     3541 aaaacacaca tacatggaaa taggaggaat agatacgttt gaaaatatag ttaatatgct
     3601 aaatggaaag aatatttatc taactataga tttagatgta ttggatgcat ctgtgtttcc
     3661 aggaacaggt acaccagaac ctggtggtgt aaattataga gagtttcaag agatttttaa
     3721 gattataaaa aactctaata taaatatagt tggttgtgac attgtagagt taagccctga
     3781 ttacgataca acaggtgtat cgacagttat agcttgtaaa atcttaagag agttatgctt
     3841 aataatatct gataaaatta aatagagtat tttatataaa tagtatatat tataatataa
     3901 gaagttattt taaaaaagtt agtttaagtt tgacgctttt ctaaagtaac ttcttatttt
     3961 tgtaattaaa aataaatata ataaacaaca aaaaagagcc ctcgatagct ccttttctaa
     4021 aatatatata tttaaattaa ataattatat tctatttacg ttttcagctt gaggtcctct
     4081 attaccttga gttatatcaa agctaacctt ttcgccttct tctaaagttt tgtatccatc
     4141 attttgtata gctgagaaat gtacgaaaac atcatcttct ccctctacag atatgaaccc
     4201 aaatcctttt tcattgttaa accattttac tattccgttt ttcattataa gaaaacctcc
     4261 gcattattaa aaaatacaaa ctaaaaaaat aagtctgttc tattaatata acataaattt
     4321 taaataaaag caaataaaat ttttaaaaaa attaattact tttagaattt aataaaaatt
     4381 g
//
LOCUS       DAMJZE010000238         4338 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJZE010000238 DAMJZE010000000
VERSION     DAMJZE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.242"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            155..1045
                     /locus_tag="PC541_003573"
     CDS             155..1045
                     /locus_tag="PC541_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2840992.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1263..2549
                     /locus_tag="PC541_003574"
     CDS             1263..2549
                     /locus_tag="PC541_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2840993.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2638..3081)
                     /locus_tag="PC541_003575"
     CDS             complement(2638..3081)
                     /locus_tag="PC541_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2840994.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3105..3899)
                     /locus_tag="PC541_003576"
     CDS             complement(3105..3899)
                     /locus_tag="PC541_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2840995.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         1684 a          492 c          675 g         1487 t
ORIGIN      
        1 attgttatta attgaaaaga tttattagtt acttgacata gtttacactt ttaattatag
       61 tataaattgg aatatttaga taattattat ttaatgtata agctaagttt ttaattaata
      121 tggttttata catttatata gaaaagaggt acgtatgagt agcacaatca aatcaaatcg
      181 aataaaaggt ataatgttta taatagcatc agcgcttggc tttgcaatga tgtctgcatt
      241 cgtaaagcta gctggagatt taccaagttt tcaaaaagtg ttctttagaa atctggtatc
      301 agctataata gctttttggc ttatcataaa acataaagga agtctaactg gtaaaaaaga
      361 aaatttaaaa gtacttttat ataggtcaat atttggaact ctaggagtta tatttaacta
      421 ctatgcaatt gacaagttga tgctttcaga tgctaatatg ctaaataaaa tgagtccctt
      481 ccttgtagta atattttgtg caattgctct aaaagaaaaa ataaatgtaa agcagatagg
      541 agctattata attgcattta taggagcttt attcataata aaaccaacat ttagtgtaga
      601 aataattcca tatcttgggg gaattgcagg agctgtattt gcagcaatgg catatacttg
      661 tttaagagtt cttggagata gagaagatta ctatacaatt gtatttttct tttcagtttt
      721 ttcattagtt actgtagggc caattgcttt tgcagtatat gaaccaatga cccttatgca
      781 acttatatat ctattactag ggggagtatt tgcaagtcta ggtcaatttg gaataactct
      841 agcatataaa tatgcacctg ctaaggaaat ctctatattt gactatagta atataatctt
      901 ttcagcaatt ttaagtatat tcttattcaa tgtctatcca gatatgttaa gtgttgttgg
      961 atatttaata gtattttcgg cagcatttta tatgttttta tacaataaga agttagataa
     1021 attagacaaa gaaaagaaga agtaaactag ataaaagtgt ttttataatt tgtattgaat
     1081 atttaagtct ttgtattaaa attaaagaaa atttaattaa aaatgtttgg tatattaact
     1141 tataacagca gttaaaattg aaaaattata actgctgttt cttttattaa aaatatccaa
     1201 atgtctgtaa aaatagacta taatagagta gtataaataa tataaagggg ataaattaaa
     1261 gtatggaaaa agtgaaagtt gctgtgattg gagcaggtaa tagaggaacg tatgcatatg
     1321 ctccatatat ttatgaaaat tctgatgtat gtgaaatagt tgctgtagca gaacctaaaa
     1381 aaggaagacg agaactattt acacaaaaat ataacctaga tagtaaaaat gtatttgaaa
     1441 ctttagaaga cttttttaaa catgacaaga tggcagatgc agtaataata gctacaaatg
     1501 atgatagaca ttatgatgta gcaaaactag ctcttgaaaa aggttatcat gtactattag
     1561 aaaagccaat gtctaatagt ttagatggtc tggtacatat agatgacttg tgtgataagt
     1621 ataaagataa gatttttatg atttgccatg tactgagata ttctccattt tataataaat
     1681 taaaagaaat tgtagaaagc aaaaaattag gagagctagt tagtatacaa tataatgaaa
     1741 atataggata ttggcatttt gcacatagct ttactagagg gaattggaga aatagcaatg
     1801 aaaccagccc tttaatttta gcaaagagtt gtcatgacat ggatattttg ttatatttag
     1861 taggaagtag atgcaaaaaa atatctgcat ttggaagctt aaagcatctt aataaccaaa
     1921 atgctagtgg agagatggca caaaattgtc ttcagtgttt agttgaaaaa aaatgtccat
     1981 attcagcaaa gagaatttat ttagaaaaag atagaagtat aaatagagct gttcacataa
     2041 atccaactga agagaatcta ttaaatatac ttaagacaag tccatatgga agatgtgttt
     2101 acagatgtga taataatgtt gtagacaata tggtaaatat actacagttt gaaaatggag
     2161 tgacggctac ttttaattta tgtgcattta ctaaagaaaa tggaagaact ataaagttaa
     2221 tgtttagcca tggtgaagtt ggtggggatt taaataaaaa tgagataaga attaaagaat
     2281 ttggaaaaaa cgaagaaata gttatgaatc catcaaatca acagaatatg gtagaggaat
     2341 atgatagaaa tcttatagct gaatttatta aattagtttc aaataaagaa ctagaaaagg
     2401 gtagagtagc agcaaaagaa gctatacaaa gtcatgtaat ggcatttgca gcagaatatt
     2461 ctagggtcag tgatgaagta gtgtatatag aagaattttt tgatagtgct aagcaaatga
     2521 ctaaggagat agaagaaact atattttaat taataaaaat ttattaaata aaataaagat
     2581 agattaaata atagaaatac gaaaatatgt gttctattaa ggaatctatc ttttttatta
     2641 aggttttttc ttatccctat caaaaaaatc catacattct gataaaagat aagaaagtaa
     2701 agcgaataag atttctgtac ttaaaggaat cgttactgaa tccataaagg tatcaattat
     2761 gttcatagct agaaaagtta gtataaaatc aattataaat tcgcaaagtt tatatattga
     2821 gtatggtaat ttgtttacat atctgaatat atctaaaata gtagtgcata gaaaatcaat
     2881 tggagtggtt atacaaaaat atattaaaaa gaaaattaat acagctccaa gagatttata
     2941 ttcaaatcct aagaatgata agaagccaat tcctataacc gtgaaaatac aaaaagttat
     3001 accaaaaaaa acagcaaaaa ataaaaatac agaaataaat tcgcctaaag gaatccttct
     3061 tttattactt atcttaatca actccttaat ttatgatatg aaatttatat aaataataac
     3121 attcttaaaa attcataata gtcctctgtt tcaaatagta ctgtttgaga atctattttt
     3181 ttcatatttg gataaaaaga ggccttaaaa gctttataat gttctctaaa ttttatttct
     3241 atcttaaatt tatctggcaa ttttattaag tgcctagata aatctccaga taaagctttt
     3301 ttgacacctt tttcaatttt ttttagtgca aggtttggat ggattgaaat tgaaccattt
     3361 ccaattcctt ttgacacagg aacagtgact atatttggat ttagtttttt tgcactctca
     3421 caaagcattt catctccact taaaaatgct acaggaacat tataatatgc agcaatataa
     3481 gcatttatag taaattcaga tgctatttca tcatttattt taaaatagtc ataatcaata
     3541 tccatagtat gggaaagtgg attaccatta caagtagctc cagagtgata gccggtgaat
     3601 atacatgcat caaagctttc atcaattcca gccatcataa ctagtgggtc tttggtccat
     3661 cctctaagta ttttgacgtt ttcaggtaaa atatttggat taatatttct accactgtca
     3721 tgtgcatctt taacaagtat ttcatgtaca ccagattgag tagctccatc acatgctgct
     3781 gttatctctt tactcatttg tgttctatag tgttgcgaaa attcacattc aagttttgtt
     3841 tcatcccaat ttacaatacc acaagtacct tctatatcag cacttaaata caatttcata
     3901 atatataccc ccttatgttt attattgatg ataataacag ataacagaaa atacataatc
     3961 aataaagggc ataaaaatat tatgtcttac tattaattat tattaaatct gtaactgaaa
     4021 aataactaaa ttggtattaa aaaatataac actatgtgtc atttatttat atagttttta
     4081 tatctgatgt ttatataaaa ttgtaacata taagtggtta catttactag tttaacatag
     4141 ttaaaatagt taggtattat ttttaagtta aattgattat tataaaatcg cagaataaaa
     4201 gaaagattta atttattaaa atgcatgaaa tgttttttcc tcaacataat gctaaattaa
     4261 actcaaaaag aaataataga aaaatcttat gtagcttaga aagaatgtga atgatttaag
     4321 ggaaaaatag aaatttat
//
LOCUS       DAMJZE010000239         4329 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJZE010000239 DAMJZE010000000
VERSION     DAMJZE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.243"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            340..2439
                     /gene="nrdE"
                     /locus_tag="PC541_003577"
     CDS             340..2439
                     /gene="nrdE"
                     /locus_tag="PC541_003577"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2840996.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2452..3423
                     /gene="nrdF"
                     /locus_tag="PC541_003578"
     CDS             2452..3423
                     /gene="nrdF"
                     /locus_tag="PC541_003578"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2840997.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3499..4125
                     /locus_tag="PC541_003579"
     CDS             3499..4125
                     /locus_tag="PC541_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2840998.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1735 a          458 c          791 g         1345 t
ORIGIN      
        1 tatattttta agctatttag agataattta aatttttaaa caggacataa taagcttgta
       61 ttgttttata tttgagttga taaaaatttc tttaaataaa aataatgtca attataaagt
      121 aataaaagtt tagatataaa aaaatttgtg tatatactat atgtagtata taatagataa
      181 agcactgact atatgtagtg cttttgtttt tgaaaaatac ttattgcatt tctataatta
      241 tagattaatt ttgggatttc aactgataag taccaaaaga aataataagc aaattaagtg
      301 aaattatggc acaagattat gttaaaagga cggtaaataa tgaattggat agagcttaac
      361 aatcaagtga taattaaaaa tgaacaaggc aagtaccaat tggaaaagga taaggaagcc
      421 ttaagtagtt acatagaaga atttatacag ccaaagttga gaaaatttaa taacctagaa
      481 gaaagattga agtatttaat agatgaaggt tattattcaa aagaagtaat aaatcagtat
      541 agcatagatg ttattaaaaa tatatatgat atgattgata aacataagtt tgaatttcaa
      601 tcatatatga gtgcaaataa gttttatcaa aattatgcat taaagagcaa tgatggaaag
      661 gaaatattag aaacatataa tgataaagtg ttaattgtag ctttgacact aggaaatggc
      721 gataaagatt tagctttaaa cttggcagat aaaatagtaa aacaagaatt tcaaccagct
      781 acacctactt ttttaaatgc tggaagaaaa agggctggag aaatggtatc atgtttttta
      841 ctatctgtag aagatagtac agaaggtata tcgtatgcta tatcttcatc aaatcactta
      901 tcaaagattg gtggaggagt tgcactaaat ttatctaaac ttagagcatc tggcgaatct
      961 atcaaagata tagaaggtgc agctggaggt gttgttggag tagctaaaat gttagaacaa
     1021 tcatttagtt actttaatca aatgggagct agacaaggtt ctggtgcggt gtaccttact
     1081 gtattccatc ctgattttga attgttgatg gatactaaaa agataaatgc agatgaaaaa
     1141 ataaggcttg ctacattatc attaggagct ataataccag ataaatttat ggagttggca
     1201 gaaaaaaatg aagtagcata tgcattttat cctcatactg tgtataagaa atatggagtt
     1261 tctcttgatg agatagaaat ggataagtgg tatgataaac tggttaatga tagtgatata
     1321 agaaaaaaag aaataaatcc taggcagatg ttgacaaaaa tagcacagat gcaacaagaa
     1381 agtggatatc catatgtagt gtacatagat acagctaata gagaacatac attaaaagat
     1441 gttggaatta ttaaaatgtc taacttatgt tgtgagatat ttcaatatca aacaccttca
     1501 gaaatagagg ggtatggagg aaaaaatgaa tggggacaag atataagttg taacctaggt
     1561 tctttaaaca tagccaatgt aatggataat aaaactatag aaagtacagt tgaaacagca
     1621 ataagagcac tgtcatttgt agcagatagc acagatataa aaccagtgcc aacggtaagc
     1681 aatagtaaca gcaagtctca ttctatagga ttaggagcta tgaatttaca tggatactta
     1741 gtaagagaaa atattttata tacttcagat gatgctatag atttttctaa tgtatttttt
     1801 gcaatggtta gatactattc aattaaagct tctatgaaaa tagctataga aagaaatcaa
     1861 acttttgaag gatttgataa atcagaatat gttaagggaa gaaatagcaa ggttttatca
     1921 aaatactatg aacaatctta tctacctaag tcagaaaagg taagagcatt atttgaaggg
     1981 atttatatac caacaaaaga agactggaca aaattattgg atgaggtaaa agaaaaaggg
     2041 atatataatg catatttaat ggcagtagca ccaactcaaa gcattagtta tgttcaaaat
     2101 gctacatcaa gtataatgcc aattactgag ccagtagaag taagaacata tggagactct
     2161 actacaatat atccaatgcc atttttaaca aatgataata tgttgtatta tcaatcagca
     2221 tacaggatgg atatgagaaa ggtcatagat ttagtggcaa cagttcaaaa ccatgttgac
     2281 caaggtatat caacaacact gtttgttaca gatgaaaaga caactagaga tatagctaga
     2341 cattacatat atgcctataa aaaaggtttg aaaagtttat attacacaag aacaaagatg
     2401 acaagagata ctcatgagtg tctagtgtgt tcagtatagg gagagaaata tatgacattt
     2461 aatttacata aaatacataa tgcagttaac tggaatagag aagaagatgg atttacacaa
     2521 gctttttggg aacaaaatgt aaaacaattt tggcttcctg aagaaatatc tgtttctaaa
     2581 gacataaagg tatggaatga actaagtaat aaagaaaagg aattatataa aaaagtattg
     2641 ggtggtttaa cattactgga tacaaaacag gggaataatg gaataccatc tatgatgagt
     2701 ttaacagaaa acctacaaag aaaagctgtc ttgtctttta tggggactat ggaagaaata
     2761 catgcaaaaa gttattcttc tatatttatg actctacttt ctaatttaga aatagatgaa
     2821 ttatttgaat ggatagaaac agaaccaaca ttacaaagaa aggctgattt agttttagct
     2881 caatacgaaa atactacaaa tcaagaggga ttatacctat caatggttac aagtgtattt
     2941 ttagagagtt tcttgtttta ttcaggattc ttttatccat tgtatctatc tggtcaagga
     3001 aagatggtag ctagtggaga aataatatcg cttattctta gagatgagtc tttacatggt
     3061 aagtatatag gattgctagc acaagaaata tacgattcat ttgataaaat ggataaggag
     3121 atgttagaag agaagatgta ttctatactt tatagcctta tggagaatga gatagagtat
     3181 acaaatgtta tatacagaga aagtgggctt gaaaaagagg ttgtgaactt tttaaaatac
     3241 aatgcgaata gagctttaga aaatctaggg tttgagaagc tatatactgt agatgctata
     3301 aatccaattg tattaaatgg tcttagcact gaaaccaaaa cacatgattt tttttcaact
     3361 aagggtaatg gataccaaaa aggagtgtat gaggaattag aggatgaaga ttttataata
     3421 taatttataa ttatgacaca ctgttgtcac ttttagcgtg ataatatcaa tgtataaaat
     3481 atatatggag gtatttttat gaaatatgaa tggagaaaaa gagataaaga gttatattta
     3541 cctaaaaaga atcctgtaat agttgaaata ccagaacaga aatttatcat gttaaaaggg
     3601 agtggagacc caaattctaa agaatttact gaagcaatag gagtacttta ttctttggca
     3661 tatgcaataa aaaccatgcc taaaaaagga attgtaccag aagggtattt tgactatacg
     3721 gtttttcctt tagaaggtat atgggataag ggagaaaaaa gtaaaggctt agatgtactt
     3781 attaaagaag atttaattta cactataatg atacgtcagc cagattttgt tacacaagac
     3841 ctgttggata aagctactga tatagtaaag aagaaaaaac ctcataatct tcttgaaaaa
     3901 gttagatttg ggtctattaa tgaagggcta tgtgttcaaa tgcttcatat aggttcatat
     3961 gataatgaac cagaaagctt tgacattatg aacgaatttt gcaaaaaaaa taaacttata
     4021 agaaaatctt acacacatcg tgaaatttat ataagtgatg ctagaaaagt atcgcctgaa
     4081 tcattgaaga cggttttacg ttttgaagta gaaagggaag tataaatatt ttgaataatt
     4141 tataagagtt ataaaaagtg aagtctgttg agacttcatt ttttgatgca attttaaaat
     4201 taaatttgat gtttcataat attttaaaaa ctttctactt ttattagtgt gaataactgt
     4261 tgctattaaa taataatact aattatataa gtattattat ataatattta tagattatta
     4321 tataaatag
//
LOCUS       DAMJZE010000240         4278 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJZE010000240 DAMJZE010000000
VERSION     DAMJZE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.244"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            477..956
                     /locus_tag="PC541_003580"
     CDS             477..956
                     /locus_tag="PC541_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2840999.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            953..1732
                     /locus_tag="PC541_003581"
     CDS             953..1732
                     /locus_tag="PC541_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841000.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1982..2482)
                     /locus_tag="PC541_003582"
     CDS             complement(1982..2482)
                     /locus_tag="PC541_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2841001.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3094..3801)
                     /locus_tag="PC541_003583"
     CDS             complement(3094..3801)
                     /locus_tag="PC541_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2841002.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         1565 a          637 c          461 g         1615 t
ORIGIN      
        1 gtttatttat gttcatctct atctaaaata atgacatttc taactcttaa ttctcattta
       61 agtttcattg ttttttctta aaagcctact tttagtcaag atactgttat ctttactcat
      121 tatttaaaga acttatttta aatctgtaca tgaagtaaat aatccaaaag cattgttatt
      181 ctaactactc aacattaaat acttactatt ttctaattac tcaatataaa gacttactat
      241 aaaactaaat acataaatgg cttctaaagt ttaatatatc tacatggctt tatatatgac
      301 aggaaaaata ctagaaaaaa tatattatag aatacttgta tataataggc aaaactttca
      361 aacttaaatt tatcaaataa tcataaaaaa agtgaaccat ctatagtaaa attttatcta
      421 ataagtgaaa ggcaaaaaag tagcgctacg atttgcatag aagggaagga acttagttgg
      481 ataaaactac atttacaaac aatatacttg aatcagaaca gactttgtat cgagtatcta
      541 agtctattct aggaaatgac caagactgtg aagatgccgt caacaatgct attttaaaag
      601 catatgaaaa gttggattct cttaaggaag aacaatactt taaaacatgg ctgattagaa
      661 tagtcataaa tgaatgtaat tcattaagac gcaaacgatt aaaatctcta tcatttgaag
      721 atgtcttcaa aaacaaaaaa attgatgaaa aagatgacta tagcgatttg tatactgcta
      781 ttcaaagtct acctaaaaaa attaaaattc caatagtcct ttactatatt gaaggttatt
      841 cagtagatga agttaaggaa atattagata tacctcaagg aactgtaaaa agtagattat
      901 caagaggaag aagattatta aaaactaaat tagaaaacac agaggtaatc atatgaataa
      961 aaatatatta aataattcgt ttccagaagt tccagaaagt tttcataata gtttaagcaa
     1021 aacactaaat agtttaccag aaagagagga aaattataaa atgattaata ataaaacaca
     1081 caaattacca tttagaaaag gcttagttgc aactttagcc attactttga tattgagcac
     1141 aacagccctt gcagtaggac atatatatag tttagttgga aaatcaagta ctaaggctac
     1201 ctatacatct atcccaacta cagaacaagt aaaaaaagac tttaacttta cccctaatat
     1261 agtttctgaa tttagtaatg gatataaatt taaaggagca tatactgtaa ataaaaaagc
     1321 atttgacaaa gaaaataatg ttttaggaaa atcaaaagca cttagttttg aatataaaaa
     1381 tggcaatgat gaaatatctc ttgacacagc aaataatgta cttgggaaga gttctgacaa
     1441 tctaaaagtt gtagatactt acaaagatat taatatttct tatgaagcat tcgcacaaaa
     1501 gtttatacca gaaggttata aaatgacaga acaggataaa attgatgaaa aatcaggcaa
     1561 atacacattt agtgtggggc caaataatga atcagacaaa gttgaagtta atgaatttaa
     1621 gtttttgaca tggaaacaag atggagttta ctattctttt acagttcaag actcaaattt
     1681 atctatgaat gacttagtta aaatggctca tgaagtaata gatgcaaaat aaacataagt
     1741 aataataagt tgtttatgag tttattattt atgactttaa tttttttatt agtaaacatg
     1801 gttgtatctt ccatctttat attttcttta aatttaatat ataaaatttt agatatttag
     1861 ctaccttcaa aagaagcatt attaaaaagc ccttaaaaat atttctttta tatggaattt
     1921 taagggcttt aactttattt tatacaagtg tatatggaat tttaaagact ttaactttat
     1981 tttatataag tgtaagctcc atttgaatta cttcttcttt tacaggtcta tcattccatg
     2041 taaaaccact agatattgta tttacctcta caaaccctaa agatttataa acatgaatag
     2101 ctctactatt acttttaaat acatcaagct caattttttc atacccatat tcttttgata
     2161 aatcaataat ttgttttaaa agactacttc cgataccttt ttccctatac tctttaagaa
     2221 tacccatgcc taaataacca actttttcag ttttaggtaa aacgtcacac caaccaatac
     2281 agttatctga ttctaaatca attataaaca gttgtggtaa gtttttattt ataatatttt
     2341 ttataaattc aactgtttct tcaaaaggaa aggcttcatc catagctaga tatttcctct
     2401 cttttgctat tttatcaaaa gtttgccaat acgaataaat atacttttct tctgccttta
     2461 taaattttat gccttctatc aacttaacat ttcccctttt taatttttga ttgtagttaa
     2521 cttattttat atgattctag tttataatgc tatataaatt actatatatt agcttaaaat
     2581 atctttgtat atttctataa aattaaaacc aatatatcta tctcttgcat ctctattaaa
     2641 ataattttta atagtataat aaatatagtt ctttaatctt ttgggtatat atacttaatc
     2701 attttgaaat ttgatttaaa gtataataaa atatataaaa aaatatataa aatggtacta
     2761 taaaattttt aatcttcatc aatacctttt ttgctttcat tgttaaaact attttttcta
     2821 ttagtttatt tcttataaaa caaacattta gtattaaaat atatatcaca caatagtatt
     2881 tgcaatttaa ctttaaaaaa ataaaaatac ccaactgacc taagcaactc gattgggtat
     2941 aaataatcta aaaaatcatt tttgtataaa ctctttattt aacttacatt tttatttacc
     3001 ttctacttta attcaactta tatttctatg cttcttatac tctcattcaa cttatatttc
     3061 tacttatctt atacttttat aaattcatca caattattta gttctcgcta tcaaagaaat
     3121 tgtattaaaa tactttccat tctctacttc catcattttt ttgtcattta tagcatatga
     3181 attatcacaa tttatccaat ccttccaagc ttcatcatga caatttaaag cttcacattt
     3241 ctctacaatc acagactccg attcagacca aagtttattc caccatgtaa ttgaatggaa
     3301 gttcatatta tcttgccaaa atggaatgag ttcatctggt atacaatcca cgaaatcttc
     3361 ttttaatcca ggcattgcca tagcaagtat tcccccctct tttacaagag gtgatatatg
     3421 attgtctaaa aatccttctt tgtttccaaa atagtgataa gaatcgatac ttatcaccgc
     3481 atcaaagaaa ccttctgcat aaggtagttc atgtgcctct gcctgtattg gaaatatttt
     3541 atcatccaat ttaaactctt taaatctttt atagttttca gttggttcta tccataaatc
     3601 tgttgcaaat acagtagcat caaattcctt tgctaaaaat atcgaagata tacccttacc
     3661 acaacctaag tctaaaatac gcattccctt ttctagtttt atcttacttg ttaattcttc
     3721 caatattttt atacaattag gtcccatcat atttttcatg agaaagtcct tatcgtattt
     3781 attacttttt gtaaatatca ttctatcttt cctttcccta tcagttatta tttattaaat
     3841 tttatacgtt tgatttttta ttttttatct gcttcttata ttgagcaatc tcttttttat
     3901 tcattcttta acagacaatt ttaatgtgta attttatcca ctctgtgata gtaactttaa
     3961 tgaataatct tatctactgt gatagttatt ttagtgagta attttatcta ctccacagca
     4021 atcgctttaa tgagtaatct tatccactct atgatagtta ttttagtgag taattttatc
     4081 tactccacag taatcgcttt aacgagtaat cttatctact ctataatagt cactttaatg
     4141 agtaatcata atttttatat cattttagat acttactcta ataagaattc attttctaat
     4201 acttaataag ttttaattac ttccacttta acctatatac taattaactt atttaattta
     4261 gtgcatattt gacacttt
//
LOCUS       DAMJZE010000241         4270 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJZE010000241 DAMJZE010000000
VERSION     DAMJZE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.245"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            57..1655
                     /locus_tag="PC541_003584"
     CDS             57..1655
                     /locus_tag="PC541_003584"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2841003.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1894..3921)
                     /gene="bclA1"
                     /locus_tag="PC541_003585"
     CDS             complement(1894..3921)
                     /gene="bclA1"
                     /locus_tag="PC541_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2841004.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGEIGPTGA
                     TGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGATGSIGPT
                     GATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSIT
                     VDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGG
                     VLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT         1266 a          770 c          656 g         1578 t
ORIGIN      
        1 tgatataata tttttatcaa ttattattat attcaagttt tgaaaggagt attaaaatga
       61 gtttattagt tttcggacat aaaaatccag atacagattc aatatgttct gcaatttcat
      121 tagcacattt aaagaacaaa ctaggaataa aagcaacagc ttatgcttta ggagatatca
      181 gaaaagaagc taaatatgcc ttggattact ttaaggtaga tgcacctgaa attttagata
      241 atgttagaat acaagttaga gatttaaatt atgataaagt agtaccttta acaccaactt
      301 catctatatt agaggcttat aaccttatgg atgaaaaaaa tgttaaaaca ctacctgtaa
      361 cttatgatga tggaactttt gctggtatca ttactatgaa agaaatagct aaaaatctac
      421 ttcatcaaca ctttacaact atacacacat cactatcaaa catttgtaaa aaccttaatg
      481 gtactattct tgtggactgt ggtgaagata taaaaggtag agtagtcact ttatcatttg
      541 gaatggagac tctaatagag actttaaaag aaggagacat agctattgtt ggagatagat
      601 atgatagtat tgaatatgct attgatacaa aagttaaact tttaatactt actaatcata
      661 cagagatttc aaaagatttg ctagctcttg ctaagataaa tggagtgtct gttgtttctg
      721 ttgaaagtga caattataaa acttctaatg taataaatca atgtagtttc ttagactcta
      781 tagaagctac agaaaattta attacattca aagaagatga ttatatggat gaaataaaag
      841 agattatgct agaaacaaac ttcagatctt atccaatatt agatgatgat aataagtttt
      901 taggtttagt ttcaaaaggt catctattaa acccatctaa aaagaatgtt gttttagttg
      961 accataacga atatgctcaa agtgctgatg gaattgaaca agcaaacata gttgaaataa
     1021 ttgaccatca taaacttggt ggtatttcta ctgatgttcc tatgtcattt agagttatgc
     1081 ctgttggatg caatagtact ataatttatc aaatgtacaa agaaaataat gttgagatac
     1141 cttatgaaat agccgggctt cttttatctg ctatattatc agatacatta ttatttaagt
     1201 ctccaacaac aactgatata gataagaaag cttgtgaaga attaagtaaa atagcaaaag
     1261 tagacatgga aaaatacgct atggatatgt ttaaatgcgg tacttcttta gatgaatata
     1321 ctatagaaga aatagtaaat atggacttta aagaatttaa tatgagtgga catagagttg
     1381 gtattggaca agtatttacc ttagacatag actctatatt tgctaaaaaa gatgagtttt
     1441 tatcttatat aaattcgact gattatgaca aactaattct tgcggttaca gatataatca
     1501 aagaaggttc ttatttaatc tataaagcag aagacagctt aattgctaac gcatttggtg
     1561 ttgaaggtgt tcaaggtaca tttgcaccag gtgttgtatc aagaaaaaaa caattagtcc
     1621 ctaatttaac tactgcaatc aaaaatttta aataatataa aatttttaaa taacgaaaac
     1681 tgtctcaaat ctatatttga gacagttttt tattatatat atatatattg ataataaagt
     1741 taaataaaaa tccataaatt taaaattttt atgaatatat tttattatta ttgttatttt
     1801 attcattcta aaataggtaa aactcaatat ataaaaatac ttttgtcatt taaataaagc
     1861 ctaaatagtt tttaagatta ttttagacac gttttaagtc ataacagtat cagctattct
     1921 aaatatcgtc aaagtagccg cagttcctgc tgattgccca tttcgtatcc ctactgttgt
     1981 agatgaaaaa ttatttattg taaaagtagt cccaccaaat aaaaaaccag caaaatatcc
     2041 agaaagtact cctccgcctc ctggctcagt agtaagccct gccaaatctt ttcctcctaa
     2101 aattgggaat aggatagcga aagaatattg atttatactt gatgaacctg gttgtaatgc
     2161 tactgaaact gatatataat acacaccatt ttcttgtact gtaaacgtac catttgcatc
     2221 tactgttata cttgaactat ttcctattat tgtttgccaa cctgttatag catctccatt
     2281 atttagtgtt tgtggagatg gtgcatttac gaggaagtta gcaactggct gagaggaatt
     2341 gcctgttgct cctgttggac ctgttgggcc tgttactcct gtcgctcctg ttgctcccgt
     2401 tggtcctata cttcctgtcg ctcctgttgg acctgttact cccgttggcc ctatacttcc
     2461 tgtcgctcct gtatttcctg ttggccctat ttctcctgtc actcctgttg ggcctgttgc
     2521 tcccgttggt cctatttctc ctgtcactcc tgtatttcct gtcggacccg ttgctcccgt
     2581 tggtcctatt tctcctgtta ctcctgtatt tcctgttgga cctgttgctc ccgttggtcc
     2641 tatttctcct gtcgctcctg ttgggcctgt tgctcccgtt ggtcctatac ttcctgtcac
     2701 tcctgttggg cctgttgctc ctgttggtcc tatttctcct gtcgctcccg ttggtcctat
     2761 acttcctgtc actcctgttg ggcctgttgc tcccgttggt cctatttctc ctgttggccc
     2821 tatttctcct gtcactcctg tcgctcctgt tggacctgtt gctcctgttg gtcctatact
     2881 tcctgtcact cctgtcgctc ctgttgctcc cgttggtcct atttctcctg ttggtcctgt
     2941 tgctcctgtt ggtcctatac ttcctgtcac tcctgttgga cctgttgctc ccgttggtcc
     3001 tatttctcct gtatttcctg ttgggcctgt tactcctgtt ggtcctatac ttcctgtcac
     3061 tcccgtattt cctgtcgggc ctgttgctcc cgttggtcct atacttcctg tcactcccgt
     3121 atttcctgtc ggacctgtta ctcctgttgg tcctatactt cctgtcgctc ctgtacttcc
     3181 ggttgggcct gttactcccg ttggccctat acttcctgtt gctcctgtat ttccggttgg
     3241 gcctgttggt cctatgcttc ctgtcgctcc tgtacttccg gttgggcctg ttactcccgt
     3301 tggtcctgta cttcctgtcg ctcctcttgg tcccattgga cctgtaattc taattggaag
     3361 acactcacat ttaggataac aattatctat aattctatct tcaaatgtta caagtatata
     3421 cggtttgtta caacctctac tagatgtaaa ttcaagaatc tgataagggt aatttaaaga
     3481 taaagctaac ccataattag gatatttatt cattgcccat gcttttatag ttcccgtaat
     3541 atttaaacaa atatagttgg atgtatcact tatcccaact ggtaaatatc ccctaatatt
     3601 ttccatagac actctatcat tccatgttat agttcgtaaa ttataatatt cacttattct
     3661 tttaatttca atatctactt tttctttaaa aatatgattc ttatttgaat caatatgaat
     3721 aaataactct gcttttaata tacttttgtc ttttaatata tctggtattc tcactttaaa
     3781 aaaagtaatc aacttttctt ttacaaaact atttgaacat ttacttccta ttaagcaata
     3841 atctaaatta ctaaagtttt tatctggata ttttttagat ataaaagtat catcatttaa
     3901 ataaagtata atatttctca tttataatcc ctccattttg ggtctatatc atactatgtt
     3961 taaaatacat gcttagttac aatataatta aaatttatag tattaaattt ttatataata
     4021 atgctgattt ctacaaaaaa agaacatgtc attagaaatt tctaatagca tgttcttttt
     4081 cattattata tattacactt taaattattc cttgactggc ttagctggtg taaactaaac
     4141 cagtctttct aaaatattaa gcactttttt cttattatgt tttttaggat ttattgtcta
     4201 tattattgta aaccaaagtt ataaaattgt aaatagaata tattaccata acaatatctt
     4261 tttataagta
//
LOCUS       DAMJZE010000242         4225 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJZE010000242 DAMJZE010000000
VERSION     DAMJZE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.246"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            69..1763
                     /locus_tag="PC541_003586"
     CDS             69..1763
                     /locus_tag="PC541_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2841005.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2095..2205
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2309..2680
                     /locus_tag="PC541_003587"
     CDS             2309..2680
                     /locus_tag="PC541_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2841006.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2775..3722
                     /gene="trxB"
                     /locus_tag="PC541_003588"
     CDS             2775..3722
                     /gene="trxB"
                     /locus_tag="PC541_003588"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2841007.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3826..4143
                     /locus_tag="PC541_003589"
     CDS             3826..4143
                     /locus_tag="PC541_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2841008.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1756 a          393 c          784 g         1292 t
ORIGIN      
        1 tattacatag tacatataaa tctatattta taaatacaat aaagtaaagt tgtaaaggag
       61 tgctttaaat gaagttttca gagtttaagt atgaaagacc caattataat agtatgaaaa
      121 aagagttttt gagttgtgta gaagatataa ataattctcg taattataaa gagcaacaaa
      181 aaaatataca taagattaat ttattaagaa ataaaataga aacgttgtca aatatagctt
      241 cgataagata tagtacagac acatttaata aattctacaa agaagaaaaa aattattggg
      301 atgaatatat gcctttgtat gaagaattaa attcatattt ttataatgcc attgtaaatt
      361 caaaatttaa atatgatttg ataaaagagt ttggagagca atttttcaca atagtagagt
      421 attctttaaa aagtttttcc aaagaaataa taagtgaatt gcaggaagaa aataaattgt
      481 gttcagagta tactagactt ttagcttcag ctgagataat gtttgatggg aaaataagaa
      541 atttatctgg tatggggaaa ttcatgtatt ctaagaatag aaaaacaaga gaattagcaa
      601 acaaagcata ttataatttc tttgaagaaa atgaaactaa atttgatgat atatttgata
      661 aattggttaa acttagagat aagatgtcta aaaaattagg ttttgaagat tttgtagagc
      721 ttgggtacat tagaatgatg agaagtgact acagagaata tatgataaaa aatgttagaa
      781 aacaagtact aaaatatgtt gttccaatgg caaatgagct ttatgaaaaa caagctaaaa
      841 gaattggtct agaatattta agttacatag atgaaggcgt ggaattttta acaggaaatg
      901 caagtttaaa gggagattca agatacataa ttaaaaatgg taaaaggatg tattctgaac
      961 tgtcaaaaga aacaaatgaa ttttttgatt ttatgttaga aaatgaactt atggatttag
     1021 aaacgaagaa aggaaaagga gctggagggt attgtactta tataccagat tataagtcac
     1081 cattcatttt ttctaatttt aatcaaactg ctgatgatat agatgtatta acacatgagg
     1141 caggacatgc atttcagttg tatatgtcta gatggattga tatgccagaa attaattttc
     1201 caacgcttga tagttgtgaa atccattcaa tgagtatgga atttataaca tggccgtgga
     1261 tggatttatt ttttaaagaa gatacagata agtacaaatt cactcactta tcatcttcaa
     1321 ttaaatttat accttatgga gtaattgttg atgaatttca acactatatt tacaaaaatc
     1381 ctaatgtaga taagtcaaaa cgaaaagaaa tctggagatt tttagagaaa aaatacttac
     1441 ctcatagaaa atatggtgat aattcttttt tagagagagg ttgttggtgg tttaaacaag
     1501 gacatatatt taaaaatccg ttttattata tagattatgt attagctcaa atttgtgcgt
     1561 tacaattttg gaaaaaaatg attcaagata gagatgcagg atggaaagat tatataaata
     1621 tctgtaaagt tggaggaaca aaatcatttt tagatattgt gagtatggga aatttgtatt
     1681 ctccatttga tgatggctgt attgagtcta ttataggtga tgttaaatct tggtttgatg
     1741 aaataaatga cagcaaatta taaaaaatta tgagaatttt attgtataaa agtaaaaaat
     1801 taaaaaatat ataaaaagtg attatttgtt gtgaaaatag tcttgttttt aactaaattg
     1861 ataaaataaa ctaaaaaaga tttgtactta aaaaaaaaag aaaatacaag aaatatgcaa
     1921 tatcaacatt tggaaaaaaa agtaaaaatg tatacaaaat acatataaaa aaactattgc
     1981 aaattttata gaatgtttat atacttaagt caacagagat agaaaaaagc tatttataga
     2041 aaacaaagga taaattgctt aattttaaat tttctgaaaa aaataataat ccggatgaag
     2101 agtatgggag actgctttat aaaaagcgac cgaagggaca agtttaatct tacttaccca
     2161 aaagattaga tgaaattctc aggtaaaggt gactgtactt ggacggggct ctgaaaatga
     2221 ttttaacagg gaagacaaat tgggagattt gtttttcctg ttttttattt atcaagtaat
     2281 aaataaaagt ttgaaagaga gtaaaaaaat gataataatt acgaataatc caaaagtaaa
     2341 agaagaagtt caaggcagag aagttttgtt taaagacaca acttatattg gaatcttaga
     2401 agcaagtaga gatttgatac atgaaggtta tgaactttta tctcatccac tctatgggag
     2461 tgtaaagcca aatgaaacac cttacaggac tgttatacta aagaaaggaa atcgtttgga
     2521 tataaactct ttaaccttaa ttgaagaagc aatcatcact gcaagtaaat ttcagaataa
     2581 taaaaagact ccaaaatgga cagaaagtgt tcaagatgat tttagagtaa ttgattatga
     2641 tatattttat aatacaatac aaagaatgca gtatgaataa aaaaattgtt aaaaagttat
     2701 taaaacagcc taaatattaa atctaattta atattaaata aaatgagaaa tttaattggg
     2761 gaggataagt caaaatggaa aatgtgtatg acttagtaat aattggttca ggaccagcag
     2821 ggcttgcagc aggtctttat ggagcaagag caaagttaaa aactctaatc ctagaaaaag
     2881 acaagactgg tggacaaatt gtaataacac atgaaattgc aaattatcca gggtcagttc
     2941 caaatgcaac aggaccaagt ttaatagcta gaatggttga acagtgtaaa gagtttggag
     3001 cagaaatgct aagagacaac attgttgata ctgagctaga tggagatata aaagttttaa
     3061 aaggggaaaa ggcagaatat agagcaaaag cagtaataat aggaacagga gcaacaccaa
     3121 gaaagattgg ttgtcctgga gaaaaagaac ttactggtaa aggggtttca tattgtgcaa
     3181 cttgtgatgc agatttcttt gaagattttg aagtatttgt tgtaggtgga ggagacagtg
     3241 cattagaaga agcaatgtat ttaactaaat ttgctagaaa agtaacaatt gtacatagaa
     3301 gacaaggatt tagatgtgct aagagtgttg aagaaaaagc aaaagctaat ccaaagatag
     3361 agtttttatt agatacagta attgaagaaa tcaaaggtga tggaatatta gaatcagttg
     3421 tatttaaaaa taaagttact ggagaaactc atgaatactt tgcagatgaa gaagatggaa
     3481 caatgggagt atttgtgttc gttggattag atgctcaaac tgatttattc aaaggtaaag
     3541 ttgatatgga tgaaaaaggt tacataataa ctgatgaaga tatgagaact aacataccag
     3601 gcgtatttgc agcaggagat tgtagatcta agactctaag acaggttgta actgcaacta
     3661 atgatggagc aattgcatct atagtagctg agaaatacat tgatgaaaaa tttgggaact
     3721 agaatctgta aattaagaaa tagtgtctgt aaatttaatg aataatattt aaaattaaaa
     3781 ttaatattta aaataaaaat aaaaatttca agggggaata agaaaatgtt agatttagat
     3841 aaggctacat ttgaagaaga agttttaaat gcagaaggtt ttgtatttgt tgatttttgg
     3901 agtgaaggtt gtgagccttg taaagcttta atgccagatg ttcataaatt agctgagact
     3961 tatggggata aaataaaatt ctgtaagatg gatacaacaa aagctagaag attagctata
     4021 aaacaaaaag tattaggtct tccaactatg gctatataca aagatggaga aaaagtagac
     4081 gaagttacta aggatgatgc tacagttcct aatatagaaa acatgattaa aaaatatctt
     4141 taatatgttg ataaattatt aacattaaaa gaaaaaatct atttttatct gaagataaga
     4201 agaaaaaggt ttttatgtcc aactt
//
LOCUS       DAMJZE010000243         4193 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJZE010000243 DAMJZE010000000
VERSION     DAMJZE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.247"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            497..1405
                     /gene="trxB"
                     /locus_tag="PC541_003590"
     CDS             497..1405
                     /gene="trxB"
                     /locus_tag="PC541_003590"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2841009.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1495..3318)
                     /gene="typA"
                     /locus_tag="PC541_003591"
     CDS             complement(1495..3318)
                     /gene="typA"
                     /locus_tag="PC541_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2841010.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3530..3772
                     /locus_tag="PC541_003592"
     CDS             3530..3772
                     /locus_tag="PC541_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841011.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3920..4111
                     /locus_tag="PC541_003593"
     CDS             3920..4111
                     /locus_tag="PC541_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2841012.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1535 a          545 c          672 g         1441 t
ORIGIN      
        1 catataaata taagtaaata gtgctaatat ttaaactatt aatcataatt ggttcaacaa
       61 aagtatatag ttaataatgt ttaatttgaa gaaattgtta gtgtgaaatt acatatatat
      121 tcaaattaaa ttcataaata tttcacataa taatagtata ctgtaaacaa aaggaggtat
      181 taaatatggg aatagtactt ataacagcag cattagtttt ggttatgtat ggactttcaa
      241 ttctatcata tatatcagaa agcaatatat tagaagacac tgtaaatgat aaccaataag
      301 gttttgaatt atataaataa gtgagaactg taaataaaag ttaattttta tttgcagttt
      361 ttttatatat atgtaaaata aagttaatta ataaaaaata atgttttgtt tatagtatat
      421 atggataata atagtaaagt ataagaatga tgagaataat atataagaaa taataatata
      481 aagagaggta aacgttatgg tagatatcat tgtaattggt gctggaccag caggtttaac
      541 ttcagcaatt tatgctatga gagcagggtt aagcgttaca gtatttgaaa aaaatattta
      601 tggaggtcaa gttgcaagta cctctgaggt agaaaattat ccagcagttc aaaagatttc
      661 tggggtagaa ttttctaata atatatacaa tcaagcagtt gctcaaggtg ttgatattca
      721 gtttgatgag gttgaagaga taaatttaga gggaaaagta aaggttgtaa aaacttcttc
      781 aggtgaacat aaagcaaagg ctgttattct tgcaaatgga gttgaaagac gtaaattagg
      841 ttgtgcaggg gaacaagaat ttacaggtag aggagtatct tactgtgcaa catgtgatgg
      901 agcctttttt aaagataaag aagttgctat agtaggtgga ggaaatactg cactagaaga
      961 tgctttattt ttagctaata attgtacaaa agtgtattta attcacagaa gagatagttt
     1021 tagaggtgaa gaagtgttgg agaaatctgt gaaagcaaga gagaatattg agatacttta
     1081 cagtcatggt gtagaaaaaa tagaaggaga aaagactgta tcgaaaatag aagtaaaaaa
     1141 cttaaagact gaagaaaaaa gaactataga tgttagtggt atttttattg ctataggtct
     1201 taaacctaat aataaaatgt ttgaaaatgt tcttgatttg gatgaaggtg gatatataat
     1261 ttcagatgag tcttgtataa catcagtaga aggtgtatat gtagcaggag atagtagaac
     1321 taagttttta cgtcaaataa taacagcagc atcagatgga gctattgcag ccgttcaagc
     1381 agctaattat ataaatgttg aataatataa atttcaaata aaaatagcca tataaactga
     1441 aataattttt cagtactata tggctatttt gttaattaga ggtaaatgta cgttttattt
     1501 accttgtcta gatttgttat atcttaatct atcatgttca tttaagaatc tttttctaag
     1561 tcttatagaa tcaggtgtaa tttcaactaa ttcatcatct tctataaact ctagtgcttc
     1621 ttcaagagta aatattctag gaggagataa ttttatagca tcatctgaac cagaagcacg
     1681 tacgttactc attttcttgt ttttacaagg gttgactacc atatcatctt ttctggaatt
     1741 cataccaatt atcatacctt cgtaaacttc tacaccaggg tctacaaaca ttacagctct
     1801 atcacttaag gcattaagag aataccccat agtaacacca ggtccttgtg ctattaaaac
     1861 accatttcct cttgaaggta tttctccttt aaattcttca aatttttcaa atgaacgaac
     1921 taaagtacct tctccacgag tagcgtttat aaattcactt cgatatccta aaagaccacg
     1981 agtaggtgct aagaactcta tttttacata gtctccttct attgacatag actccatcat
     2041 accttttctc atgtttaatt catttataat agtaccagag taaacttcag gacagtttac
     2101 aacaactctt tctattggtt ccattaattt tccatcttct ttatgcatta aaacttcagg
     2161 tttagaaaca ccaacttcat aaccttcacg tctcatattt tcaagtagta tagataagtg
     2221 aagctctcca cgtccagaaa ctttgtatcc atcagtagta tcaagaggtt caactttaag
     2281 accaacattt acttcaagtt ccttttcaag tctatctttc aaatgtctag ttgtaacgaa
     2341 ttttccactt tttccaacga atggagagtc gtttactaag aagttcatag aaagagtagg
     2401 ctcttctatt tttatcattt ccataggtag tggactatct aaatcgcata tagtttcacc
     2461 tatagatata tcaggaatac cagctataac aactatttct ccactagtag cttcatcaac
     2521 ttcaacttgt tttaaacctt catacacaga aagttttgat acttttcctt tagatacaac
     2581 agaatcttct ctacatatag caacttgtgt attgttttta agagtacctt tgtaaattct
     2641 acctatacca agtctaccta cataatcatc atatgcaaga gctgatattt ggaactgtag
     2701 aggttcatta tcgtaattag gataagcttc aacgtgattt acaatagttt taaatagagg
     2761 agaaaggtct tcactatcat catccatttc aagtttagca ataccttgtt tagcaatacc
     2821 atatattata ggaaattcgc attgctcatc tgttgcattt aagtctacaa ataaatcgaa
     2881 aacttcatta actacttctt ccgctctctg gtctttttta tcaatcttgt ttataaataa
     2941 tattggtttt aaaccgaact ctaaagattt ttgtaaaacg aatctagttt gaggcattgg
     3001 gccttcacta gcatcaacta atagaataac tgtgtcaaca gttttcataa cacgttcaac
     3061 ttcagaagaa aagtcactgt gtcctggagt atcaactatg tttattttgt aatcttcata
     3121 gtttattgca cagttttttg aatatatagt tatgcctctt tctttttcaa ggtcgttact
     3181 gtccataacg cagtctttaa ctacttcgtt ctttctaaaa acaccgcttt gagaaaggaa
     3241 agcatcaacc aatgttgatt ttccagcgtc aacgtgggct ataactgcga tatttataat
     3301 tttatgtttt tgtgacattt ttgttgctcc cttcgattaa aataacttat taattatagc
     3361 acaatatatt attaggtaca aggaggaata tattaaattg tattaatata gtttaaattg
     3421 aataaaactg ttagttttgc atctgaaaat tattttgttt aaaaaaattt tttagacaaa
     3481 taataacctt aatagataaa aagattaata aaagagtaaa ggatgatata tgttggaagt
     3541 aaccaaaata ataattaaat attttgcaag tgaaagtgat acttttgtaa aagaagaagt
     3601 ttgtaatttt gaagaacttc ttaagtggtc tcataaattt attgaaggta aaaagataga
     3661 agatgttttg tatataaagt ctaaagaaaa ctataaagta aatgtgatta agtctttaga
     3721 aattaaggaa ttggaaatag tgagaaaaca acagatacca ccgtacatgt agtattaata
     3781 atatataaaa ttaatatgta atactcgcaa taggatataa atggtatata tggtaataat
     3841 ttatattaaa aaaactttta taaagtttat atttgggtat aaaagtatta attagacatt
     3901 aattaggagg aacttaaata tgataagagt atgtccaaac tgttcaaatg tggatgtaaa
     3961 taagctaaaa actttggttg gagaagaaaa tgtaaagact ggttgtatag gtcagtgtag
     4021 agcatttaaa aaagaagctg ttggaataat agacaatgag cttgaaataa aggaaacaga
     4081 agcagagttt tttgaagcat gtaaagaata aattttaaat aaaatataat aaatagagca
     4141 tctaaattgt ataagcatta aacaatttag atgctctatt tttatatata cat
//
LOCUS       DAMJZE010000244         4143 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJZE010000244 DAMJZE010000000
VERSION     DAMJZE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.248"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(171..1088)
                     /locus_tag="PC541_003594"
     CDS             complement(171..1088)
                     /locus_tag="PC541_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2841013.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1350..1727
                     /locus_tag="PC541_003595"
     CDS             1350..1727
                     /locus_tag="PC541_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2841014.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1883..2332)
                     /locus_tag="PC541_003596"
     CDS             complement(1883..2332)
                     /locus_tag="PC541_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2841015.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2496..2900)
                     /locus_tag="PC541_003597"
     CDS             complement(2496..2900)
                     /locus_tag="PC541_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2841016.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(3013..3879)
                     /locus_tag="PC541_003598"
     CDS             complement(3013..3879)
                     /locus_tag="PC541_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2841017.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1358 a          688 c          455 g         1642 t
ORIGIN      
        1 aactaacatg taatgttagc tactctagat atgaaatctt aaaactttta aatattgaaa
       61 tatttcttta tatatattat tgtatatttc ttataaatta ttgtcaattt attttttctt
      121 aaaaataaaa taattgcata atttatttca aattatgcaa tttataaaat ttattcccaa
      181 aactctttta ctgcttcgat tatctcacta taaggcaata aagtcgcttc gattattgat
      241 ttatttgtct tttctaaata atacttaatc atataatatc cacaagcata tcctgcacaa
      301 taaggcaacc ctactggaaa atatccttgc attttagcta tatcatcacc ataaagataa
      361 gatgttatat tttgaaatcc agtttctttt aaagcacttt ttattattgg ctttatataa
      421 gtatttaatg tttcgatatc tgttctactg acccaaggtc ctaacatttc ctctccaaac
      481 atccatgttg caaaattttc tgcaagacct tcatttatca tcatttcttc taatgttata
      541 ttattattcc actctataaa ctgaaatcta atattgtgat tacattcatg tgctattaat
      601 actggtaatc tattgatagt atattcatta ggaaccaatg atagaaatat atatccagga
      661 atcccaccat caccccagta tccatcactt aatattgtat aaggactatt tggattcgcc
      721 aataatattg aatatttata gtccttaatg tttaaatcat acccttcttt tataaaagca
      781 tttatagagt tttctatggt ttctttgcaa gtgccccaaa ttttatcagc agataacaca
      841 tttatattct ttttttgctt ttcatcaata tccttaggcg ataaaacccc taacatttca
      901 cttgctataa ttacatcata tccacctttc tgtctagcaa ctattggaac attcatacac
      961 ttccacttaa attcaaaagg cttcattaat tcatatctgt atatatcttc ttttttgttt
     1021 attggtgcat ccattatctt cttgtatatt ttatctgagc gaatttctat aatattcata
     1081 tttaccatct cctttaagac aaagtatata caattacgta gcgtcaatgt caatatacat
     1141 tttttataat gtcaccttaa tctttttatt atattttatc ctgtaaacct tccgtacata
     1201 taacattttt ttaaattaat aaatttgtat aatataataa taaaaaatta tatacttcaa
     1261 ggtttcccta tgatacaatt tactaaatca taatttagat tatcaggtgc aactataaat
     1321 tattctatat caaggaggat atattggtca tgctaactcc atatttaata tttaatggta
     1381 cttgtgaaaa agcatttaat ttttatgctg aggctttcgg aggaggaaaa actatatttg
     1441 cgcgattaga cagcaatcca aacaatccta ttatgcacgc aagtgttact ttcacaaaat
     1501 acgaaggttg tataatgggt gcggatacag acaagcctgt tgtaatttct ggcatggcga
     1561 tttgtgttgt tctaccatct cgagaagcga tagaagaaat atctgtaaaa cttgccgaag
     1621 gtggtacact tgtacaagaa tttttaccac acccaccacc acatcaaaat gatggcgctg
     1681 ctgaagtact tgataggtat gggtatactt ggtatttaag tacatagtta aaataaaaaa
     1741 actacatcta ataatcataa tatagaacag ataaatttca atttaatagt gtagtaaaaa
     1801 agtcgtgtcc taagactgtc acgactttta tttgtataaa aatttaagta aatatttttg
     1861 cgaataagtg ggaagtataa ctttaaatta atttcatatg tagtattgga aatgggttcc
     1921 cttgtccatc tatttcagac ctatcaaaca ctttaaatcc taagtgaata taaaatccta
     1981 cagctttttt attctgctca tttacatcaa catattttac attgtattta tctatagcat
     2041 attttattaa ttttttacca ataccatttc ctctatattt atcttctata aagagcatct
     2101 caattttatc tttatctatt cctataaagc cctttattat atcattatct tttatacaaa
     2161 ctatattttc tacttcttta agtccaatct caactaatgg tatcaaagat tcaatatctt
     2221 tttctgttaa aaaatcatgc gttgctctta ctgatgattc ccagacttgc aacatttcgt
     2281 catattctgt tgaatttatt tgtttcattt caagaattat tccagatttc ataaaaattt
     2341 cctccctata tcacttttaa ttgcataaca cttttttata caaaacttag aaaggactac
     2401 gaaggttaaa tatcatagtc ctcattaagt ttaaatatta tctattatat attatactat
     2461 aatctagcaa attcactaca ataattcaca tcagcttata ctaaaggtgc ttctatttga
     2521 gtagtaaaaa ggaatttatg acaatgacca tcattaacag tagtttctcc acatattaag
     2581 tgaatgtgtt tatcagttcc aggtattcta attgctggcc ctgttgtaac acatatctta
     2641 tggaaatggt caaggaaatc tacattatca tttattttat gaacatgatt tgtacctcca
     2701 tttattggta taacttcacc agtaacccct gctgcacggt ggttatgtct atcctcacat
     2761 tcttctgcta acttaacact ttctgaatat tcatgaacat gtgtcatttc ctcataatca
     2821 ttatagcact tgttgcactt attgtattta taatcatcat acacataatc tctttcacat
     2881 ttataatttt gataactcat ttttatatct ctccctatat tatattaaga aattcttgta
     2941 aagaatctct aatgtataat atgaattaaa gtcaaaaaag ttcatatttt gtaaaattat
     3001 gtattaattt aattattttt cccagtattt agggcgtatt agtgaagctg gtaacttcgt
     3061 actagaatct ccttttgcag tatttacttg taattgtgtt aaaaatatac tattttcaag
     3121 attagcacct ctaatatcac aatctcttaa gtctgctcct attaaatttg ccccactcaa
     3181 atctgtattt cttaaatttg ctgctattag aaatctacct cttaaatctg ctcctattag
     3241 atttattttt ctaaggtctg ctcctattaa atctatattt ttacttaatt ttttcatgtt
     3301 tttaagagta gaagtcttga taaatgattt aacctttccc atcatagact cactcgcttg
     3361 tgataataat aaattaacct ttgttctatg tgatatgata tccaaattaa ttatctgttc
     3421 tgggcccata ttagttatct tttcagtttc ttcaattatt aaatcaattt ttgatttgat
     3481 tgtatctgat atatttaata cagatgcttc tttaagatac cataacattt catgtaattg
     3541 tctcataatt aaaaacactt cagacatttg atttgttagt tctggtgatt gcatccagtc
     3601 tatcccctta tatgttactt gtgctacttt ttgacctgaa ccaaaacaat catatgcagt
     3661 acacccttta aaacctcgct taataagact attatgtaca gaacatttaa aatctggttg
     3721 taaattaata cagggttttc ctgattcctt attgatagga aatccatctg aagcagaaaa
     3781 ataaagagct acacagcaaa gtccaaagca cttcttacag tcaatcttta gctcctcaaa
     3841 taacttatta tcatatatat ttttatgtat atctatcata ttttttctcc tcttaaactt
     3901 tatttttaat attatattat ctataactaa atgataatca agtcccttat ttaacagttc
     3961 ttattcttaa ttataattta aggtattatt tattccaata gcatacataa gtttctttat
     4021 acttgtaaca atacctttat actaagactt attttaaatg tagcactgta ataataaatt
     4081 gtttatatac tcataatgta tttgataaat tgtaaatctt tatagaagtt gaaattttta
     4141 atg
//
LOCUS       DAMJZE010000245         4139 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJZE010000245 DAMJZE010000000
VERSION     DAMJZE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.249"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            164..1567
                     /locus_tag="PC541_003599"
     CDS             164..1567
                     /locus_tag="PC541_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2841018.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1658..1898
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1941..2639
                     /locus_tag="PC541_003600"
     CDS             1941..2639
                     /locus_tag="PC541_003600"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2841019.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2662..3663
                     /gene="trpS"
                     /locus_tag="PC541_003601"
     CDS             2662..3663
                     /gene="trpS"
                     /locus_tag="PC541_003601"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2841020.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1556 a          457 c          736 g         1390 t
ORIGIN      
        1 atttttaata agatttgatt tcaaagtatt taaggtttat tattaaaact gtttttgaat
       61 atgcaaatat tttgaaaaat atcagtttaa taataatgaa tctattttat taattttgaa
      121 attaaattta aaataattaa tagagagggg aaattaaatt aggatggaaa agaaaaaaat
      181 aggcctatgg agtttggtat ttatgaatgt atcagcttta tatggtataa gatggatagc
      241 taaatctaca gcatcaagct ttggtttagg attaggtgca atacctatgt ggttattatt
      301 ctcatttata tactttgtac caggagcttt aatttgtgct gaattagctt ctacatatca
      361 aaaaggtgat ggaggtcttt acgattgggt taaggaagcc tttggagaaa agtatggatt
      421 tttagtttct tggttaaact ggacagctaa aatattttgg tattcttcat ttttaacttt
      481 cttagcagta aatgtttctt atacaatagg aaatccagcc cttgctgata ataagatgtt
      541 tgtacttata ttatctttaa taatattctg ggcattatct cttatagcaa ctaaaggaat
      601 aagttttaca aaaatattta ctaatacagg tgcattaggt tcaacaatac caagtgctat
      661 acttataata ttttcatttg tagcagtatt tgtgttgaaa aaacatgata tagcttctac
      721 atacacaata gcttcaatga ctccaaaact taatatggat gcatttgttg ctatatcagc
      781 tataatgttt ggtttagcag gagcagagac tgctgctaac ttcataacag aaatagataa
      841 gcctgaaaag aacttcccta aggcaatatt aatatcagct ggaatagtag cttcattata
      901 tgtattaggt tcaattgcaa taacaatgat aataccacca gaccaaatta ctgcttctaa
      961 aggtgtatta gatgcacttt ctgctgtttg tgcgtcttta ggaattggtt cttggttggt
     1021 tcaattaata gcttttggta tagctttttc agtattagga gcaatagttc tttacatagc
     1081 atcaccaata aaaatgttat ttggtagtgt taaaaaagga atatttccag atagtttagt
     1141 agaggtaaat gaacacaaga taccttctaa agcagttata cttcaagcaa taatagttac
     1201 tataatacta ttggtgacta cattaatgcc ttctgttgat gctatataca atgttcttgt
     1261 aactatgaca gcattaactg cattattccc atatgtattg ttatatgctt cttatattaa
     1321 actaagaaaa gagagaccag atgaaattag accttatact atggcaaaat caacaagtac
     1381 ttgcctaggt ttagcaaaaa tggttttagt tgtaactgta gtgggtatat tattatctgc
     1441 agcaccagtt atgcctacat ttgctgaaaa tgttatatat gagatagaga tgattggtgg
     1501 aggtcttcta gttatattat ctggtctatg gttatggaat agatatgaaa agaaaacagc
     1561 taaataaaat gtattttaaa aatttctttt ggatattata atatatagtt gacaatttat
     1621 agtgtaagga atattataaa ataaagaaat taataaatta aatcctgaga taagaaatag
     1681 taaataaata tatttttgca cagagaatta aggatgctga gaacttaata aaaagtattt
     1741 attgaatggg tctttgagga gtaaaatgaa atgtaattta atattacaga gtatttgata
     1801 tcgtgtatgc acgttatagc atatattaag atatatatat tcaattttga gtgtatataa
     1861 atttaggtgg taccacggaa aatatatccg tcctattgat ttaggacgga ttttttaata
     1921 tagaaaattt aggaggaaaa atgaacgtaa taggaattat aggtgcaatg gatgaagaag
     1981 ttagtatttt agttgattta atggatatta gagagactat aaaaaaagct agcttagaat
     2041 tttataaggg aattttggaa ggtaagaatg tagtgttagt aaagtgtggc ataggtaagg
     2101 ttaactctgc tctttgtgcc cagatactaa taagcgaatt taaggttgat gctattgtta
     2161 atacaggtgt tgcaggagct ttaaacgaaa aacttgatgt taatgatata gtaatatcaa
     2221 ctgatgctat acagtatgat gtagacacta cagcatttgg agaccctaag ggagttatac
     2281 caagaatgaa gacttctgta tttaaagcag atgaaagact tatagatgct gcttacaaat
     2341 cttctgtgga agaagtaaag actcataaag tattaaaagg aagagtagtt acaggagata
     2401 aatttataaa ttctaaagaa ttaaaagaag aattagtaaa tgattttggt ggatattgtg
     2461 gagaaatgga aggtggagct atagctcatg tatgctattt aaataatact ccatttgtaa
     2521 taataagagc aatgtctgat aaggcagatg gaagtgctga tgttacttat gatgtatttg
     2581 tacatgatgc tgccaataac tctaaagaca tagttttaaa tatgttaaaa tcaatataaa
     2641 tatataagga ggtacaaaaa aatgagtgaa aaaaaagtta tatttagtgg tgcacagcca
     2701 tcaggcaaaa tgactttagg aaattattta ggagctataa aaaactggac tgaacttcaa
     2761 gacaattatg attgttatta tagtatagtt gatttgcatg caataacagt acctcaagac
     2821 cctaaagttt taagagccaa tactatagaa ttacttgctc aatatatagc ttgtggatta
     2881 gacccagaaa aaaatactat atttatacaa tcacatgtca aagagcatgt tgaacttatg
     2941 tgggtattga atactatgac ttatatgggt gaattaagta gaatgactca attcaaagat
     3001 aaatcacaaa agagtgaagc aaatctaaat gcaggtttat tcacatatcc agttcttatg
     3061 gctgctgaca tattgttgta tcaaactgac ctagttccag ttggagatga ccaaaaacaa
     3121 catttagaat tagctcgtga tttagcaaat agatttaata atagattttc acctactttt
     3181 gtagttccag aaggatacta cccaaaaggt ggagcaagag tgatgagttt gcaagagcca
     3241 actaaaaaaa tgagtaaatc cgattcaaat gaaaatgcat ttatactatt agcagatgat
     3301 tcagattcta taaagagaaa gataaaaaga agtgttactg attctttagg tgttgtaaaa
     3361 tacaatgatg aacaaccagg tattaaaaat ctaattgata tatattctaa tttaagtaaa
     3421 aagagtgtag aagagattgt aaacatgtat gaaggaaagg gttatggcat atttaaagaa
     3481 gatgtggctg aagtagtatc agaagcttta agacctataa gagaaaaata tgttgacctt
     3541 cttaataata aagattattt agaaaaaata tatagtatgg gagcagaaaa agccgaaaaa
     3601 caagctagaa aaactcttag aaaagtttat aaaaaagttg gatttataga gagaagatac
     3661 taattattag ctatctttac ttgataaata aattttaaag ttattaaaaa ggtatatgat
     3721 atgaaagaga gagtctcaaa gtgaactttt gttcatgagg cactcttttt tgtatgaaat
     3781 aaaatatatt ttaatcattt caacaatgaa aaagaggatt atctctattt tgagacagca
     3841 tcttttgata ataaaaaaag atgagctttt gctcatctca aaggggttag attattctta
     3901 ctttggggaa agtagaataa aggtaaaagt taactgatgt caactttgtc ttttttgcat
     3961 atcactactg agcataaaaa aatcagaaaa ccatagcctt ctgaaacaac ttctaacata
     4021 cattgtattt tatacccaat atgtaattta tgagttaatt ttaactctta taatgaaaat
     4081 tgtcaataat atatcaaaaa aatatttttt aaatttatat aattaaatga atttatgtt
//
LOCUS       DAMJZE010000246         4101 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJZE010000246 DAMJZE010000000
VERSION     DAMJZE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.250"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..2321)
                     /gene="carB"
                     /locus_tag="PC541_003602"
     CDS             complement(<1..2321)
                     /gene="carB"
                     /locus_tag="PC541_003602"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2841021.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
     gene            complement(2514..3563)
                     /gene="carA"
                     /locus_tag="PC541_003603"
     CDS             complement(2514..3563)
                     /gene="carA"
                     /locus_tag="PC541_003603"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2841022.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(3820..>4101)
                     /locus_tag="PC541_003604"
     CDS             complement(3820..>4101)
                     /locus_tag="PC541_003604"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841023.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
BASE COUNT         1177 a          811 c          415 g         1698 t
ORIGIN      
        1 tccattatac caggtatcaa tatatcttct ttatcacata tagcatctac ttctatttct
       61 ctaccagtta gatatttatc tataagtact ggatttttat ggtctctatc aaatgcatca
      121 tccaaatact gaattagttt ttcttcgtta taagttattt ccataccttg accaccaagt
      181 acataagatg gtctaactaa tactggataa cctaactcct tagcaatttc aacaccttca
      241 ttagttgtcc atacaccttt tccttttggt ctatttatat ctagtctctc tagtaaatca
      301 tcaaattttt ctctatcttc tgctgcatct atatctctaa agtctgttcc cagtataggt
      361 atattttttt catgtaaaaa cttagccaat tttatagctg tttgtccacc aaactgtagt
      421 attacacctt ctggtttttc tttttctatt atatttaaaa cttcttcttc tgttaatggt
      481 tcaaaataca acttatcaga tgtatcaaaa tccgtactta cagtttcagg gttattgttt
      541 acaattatag tttctattcc catttttctt aaagatttta cacagtgaac tgaacaatag
      601 tcaaattcta ttccttgacc tattcttatt ggaccagaac ccaatactac aacttttctc
      661 ttatcactaa ctactacctc atcatattgt tcataagttg aatagtaata aggtgataga
      721 gcttcaaatt ctcctccaca tgtatcaacc attttataaa ctggttgtat attatataaa
      781 cttctcaatt catataattt ttctgggctt atcttcatta agtcagatat acctttatca
      841 gaaaatccct ttttctttaa ttcatgtaaa tattctttat ctaagtcttc tatttttaga
      901 acttttaact tttcttcttg ttctactatc catttaaatt tatttataaa ccacttatca
      961 acaccagtta tttctgatat catttctact ttatagcctc ttcttatcat ttcagctaag
     1021 tcaaatagtc tctcatcatc aggtactact actctagcct ttaattcttc taatgttcta
     1081 ccttctgatg gagcatgaac caatgaatat tttcctgttt caagtgatct tataccttta
     1141 agaatagcag cctcaaagtt acttcctata ctcatgattt caccagttgc catcatctta
     1201 gttcctaatt ttctattggc atgtctaaat ttatcaaatg gccactttgg tatttttact
     1261 accacataat ctaatgttgg ctcaaaacaa gcataagttt ttttagttac tgcattttct
     1321 atctcatcca aagtataacc taaagctatc ttagctgcta cttttgctat tgggtatcct
     1381 gtagcttttg atgctagggc tgatgaccta ctaactcttg ggtttatttc tattatcgca
     1441 tattctaaac tatgtgggtt taaagctatt tgaacgttac atccaccttg aacctctatt
     1501 gcatttatta tgtctattga tgcctttcta agtagttgat actcttcatc acttagagtt
     1561 tgacttggag caacaactat actgtctccc gtatgaactc caactgggtc tacattttcc
     1621 atattacata cagttataca gtttccattt ccatctctca ttacttcata ttctatttct
     1681 ttccaacctt ttatactctt ttcaagaaga acttgtccaa caggacttag ctgtagtcca
     1741 tgtgaaagta tttctcttaa ttcttcctca gtatcagcta tacctccacc agttccacct
     1801 agcgtatatg ctggtcttac aactactgga tatcctatct tattggcaaa ctcaagacct
     1861 gcttctaaat ttgtaactat atcactaact attactggtt gatttatttc tttcattact
     1921 tctctaaata aatctctatc ttcgcccttt tttatagatt ctatagatgt tcctataact
     1981 tttacattat atttatctaa tataccagca tcatgtaatt ctacagctaa gtttaatcct
     2041 gtctgaccac ccatacctgc aagtaaactg tctggtcttt ctttttctat tattttttct
     2101 ataaattcta ttgttaatgg ttctatatat attttatctg caatttcttt atcagtcatt
     2161 atagtagctg ggttactatt tactaataca acttcaattc cctcttcttt taatgcttgg
     2221 caggcctgtg ttcctgaata gtcaaactct gcagcttgac ctattattat tggccctgac
     2281 cctaatacta aagttttctt tatactatct aatttaggca taactctttc tccttatcta
     2341 aacaattttc acagttctta tcttcatcat ctatacaatc aactttaatt actttatttt
     2401 atcttaagtt caaatttact catcaataca tctatttaat acatctatgt taattaaacc
     2461 aaactttttg atttaagtta ttcttgtttt ttagttagct attctaatgt tgtttatttt
     2521 actaattcta aaaatttatc aaaaatatag tcattatctt ttggtcctgg gcaagcttca
     2581 ggatggtatt gaacactgta tacatctagc ttctcatgtc gcattccttc aacagtatta
     2641 tcatttaaat ttatatgtgt cactttcatt tgttcaggaa cttctgaaac ataataacca
     2701 tgattttgag aagttataaa tacttttcct tcttctaaat ctttaactgg atgatttcca
     2761 cctctatgcc caaatttaag tttagcagtt tttcctccaa gagctaaagc taaaagttga
     2821 tgtcctaagc atattccaac tataggtttt ttaccaataa gagcttttat attttctata
     2881 acatcttcta agtcttctgg gtctccaggt ccatttgaca agaatattaa gtcagggttt
     2941 atactcaata catcttctgg agatgttgta gctggaaata ttgttatatc acatccacga
     3001 gctataaatg atcttaatat atttcttttt actccaaaat ccataacagc cacttttgga
     3061 ccatttcctt ttatatgctc tacttctttt cttgtaacag ttctaactgc ttctgtattt
     3121 gaaaacttat ccaatttatg tttaacatct tctaatttac tgtcttctaa tgtaattata
     3181 cctttcattg ttccattatt tctaagtatc ttagtcaaag ctcttgtatc tattccttca
     3241 agtccgataa ctttattttg ctttaaatat tggtctatat caatttcaca tctaaaatta
     3301 cttggagaat cacttttctc ccttactatg aatcccttta cttgaacttt ttcagactct
     3361 gctgatgata aatttatacc ataatttcct acaagaggat aagtcatagt aactatttga
     3421 ccatagtaag aagggtctgt taaaacttca ccatatccta tcatcgaagt attaaatact
     3481 acttctccta cactttcttc taaatatcca aatgcctttc ctataaaaac tgttccatct
     3541 tctaatatta accttgcttt catactacag tacctcctat aattctcttt agacaaacta
     3601 aagaacatta actaaatata agcttttaaa tatctatatc tatttttcac ttttacattc
     3661 tagttttata cttatttaca tttatttttt atctttttct attatactac aaatttctta
     3721 aaaaaaagaa gttatctcaa aagtaaaaac tcctagaaaa aataacctct tttttttatt
     3781 taataattaa attcttaaac tatacaatca acttaatttc tattcagcta tattgtaaac
     3841 ttttaactca tctttattta aatgtttctt cttaacttct actaaagctt ttagtgtatc
     3901 caaagatgtc attatttcag tagaaaattc aatagctgtt cttcttatct taaatccatc
     3961 tcttgtagag tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct
     4021 tatagcatct aatatgtttg gtctagattc ttctactcta tttactatat cagcctctat
     4081 tccattttct cttaagctat t
//
LOCUS       DAMJZE010000247         4052 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJZE010000247 DAMJZE010000000
VERSION     DAMJZE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.251"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..394
                     /locus_tag="PC541_003605"
     CDS             <1..394
                     /locus_tag="PC541_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2841024.1"
                     /translation="KKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVKIN
                     LNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFGNM
                     KSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            382..1320
                     /locus_tag="PC541_003606"
     CDS             382..1320
                     /locus_tag="PC541_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2841025.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1704..1928)
                     /gene="secG"
                     /locus_tag="PC541_003607"
     CDS             complement(1704..1928)
                     /gene="secG"
                     /locus_tag="PC541_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2841026.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2521..3813)
                     /gene="eno"
                     /locus_tag="PC541_003608"
     CDS             complement(2521..3813)
                     /gene="eno"
                     /locus_tag="PC541_003608"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2841027.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1396 a          653 c          453 g         1550 t
ORIGIN      
        1 aaaaaaaata gaacatgtgg tcattactaa taataatata gaaaattata tcaattttta
       61 taaaaaattt ggtttttcat ctaaagaaat gagcaaaaat tataaactta atgctagaaa
      121 tgttaagata aatcttaaca taaaaggtta taaattttta ccttatgtta aaaattcaaa
      181 agcaagtaga gataacttat gttttaaaat aaacacaaat attgatgagc caaaaaaata
      241 cttagaacaa aatgagattc aaattaagag aggaattgta gaatgtacag gtgtctttgg
      301 taatatgaaa tctatttatt tgaaaaaaac taatggaaat ttaattgaat tatcaaatca
      361 tagaatcacg gagagtaaac cttggaaaat ttaatcctat caataaatgt tgttcttcct
      421 ttgtttctca caatgtcact tggatatgtt ctaaaaaaat taggtctatt tgatactcat
      481 actttaaata aaatgaataa tgtttgcttc aagtctttct tgccattatt attatttttt
      541 aatatataca atacagattt agatggttct cttaatttta agcttatggc tgtagctgtt
      601 ataattattt tttcaatctt cttaatctta gtctttatta ttcctaaaat tgaaaaagat
      661 aataagacaa ggggtgcatt aatacaaggt gtatttcgta gcaatttcgt tatatttggt
      721 attcctgtga caatttcact atttggcgat aagagtattg gagtcacatc tttattaatt
      781 gcagttattg tacctatgtt taatgtatta tctgttgtaa ttcttgaaat ttttagaggt
      841 ggcaaagtaa atataaaaca tatgattaaa agcgtcttaa caaatccact cataatcgcc
      901 tcttttatag gattgacaat gctaatttta aatattaata tacctacatt tttacacaaa
      961 actattggtg atatttctaa aatagctact cctttatcac ttataattct aggcggctct
     1021 tttagttttt ctgctataaa ggggcatata aaacccatta tattgggcgt atgtggaaaa
     1081 cttatacttg tacctgctat atttttacca attagcattt ttcttggatt tagaaatgta
     1141 gaattagctt cacttatgat aatgttatct gctccaactg cagtttcttc atttacaatg
     1201 gcacaacaga tggattctga cagtgaatta gctggacaaa ttgtagtatt tacttctgga
     1261 ttttctataa ttacagtatt tttaataata tttattttaa agcagaataa tcttatttaa
     1321 tgatatttga taacacaaat tggtattata agaaacatat ataaaactga tttaatcgcg
     1381 taaaaacata ttattatact atttttacta tataatataa ctttgtgtat attatctcat
     1441 taaacatggt gaaagtagtg ttgctatata ttttttagtt tgaagaaaat atataaatac
     1501 aaacaatata taaatacaaa taataaacgc caccaaatag tcaatctata aaataaaaac
     1561 tggactaatc atataaaatc attagtccag cttttatttt acgtactttt tatgttgatg
     1621 tacttttatt aaaaactaaa tttaattttt aaatgtctag caaatgcact aattaaataa
     1681 tggccagtta ttcaacaata tcactacttc ttaactataa gtaatgccaa tgcatttata
     1741 aaaaataata ctgctgttat ctttgtaact ctagcaagaa atgcttgttt tccttttggt
     1801 ttaaagtaag cttgacttcc ttctccacca aattctgaag gtaaagcatt cttagaatct
     1861 tgaggcatta tgctaattac tagtaaaatc cctagtacta cttgcacacc cattaaaatg
     1921 ttgctcattc ttgcccctcc tatctgttta ttacttaaat ttcattactc tttagtgtat
     1981 cacaataata tagccatttc aagactatta tgctataaat aacatacaaa aaaatatatt
     2041 gttactataa tatattacta tattctcaat ataaaaatga ataaaactta gaagagaaaa
     2101 ctatttaaat taatacataa attaacactt aaaataatct actctcattg tatatattat
     2161 aaatagccac caaaataaaa caatcatttt aaaatattga tataactttt aatttcaaca
     2221 taatttaaaa taaataatcc acatttagca ataatacttc aatgacaatt atgattaaat
     2281 caaaatatgt cttcactttt attttttaat aaaaacctcc tatgcaatta gataaaaatg
     2341 agatagaaac atttattcat attttttctg aatctatgaa taaaattaag taataaaata
     2401 taaaaaagga taagtgaatt gaaaaatcaa ttaacactta tctcttttat atcttataag
     2461 atgagcttaa atatcttata ttctctcttc attaatcttt tttagtaacc attttaataa
     2521 ctattttttt aagttataga aagattttaa tccacaatat ctagcttgct ctccaaccat
     2581 ttcttctatt cttaataatt ggttgtactt agcaactcta tcagttcttg atggagcacc
     2641 agtttttatt tgaccagcat ttactgcaac agctaaatct gctatagtag aatcttcagt
     2701 ttctcctgat ctgtgagata taacagcagt atatcctgct ctcttagcca tttctatagc
     2761 atctaaagtt tcagttattg tacctatttg attaacttta actaatatag agttagctac
     2821 tccattttca attccttttt ctaatctctc tgtgttagta acaaataagt cgtcaccaac
     2881 taattgtaat ttatttccta atttttcagt taataatttc catccatccc aatcttcttc
     2941 gtctaaacca tcttctatag ttattattgg gaagttgttt gaccaatctt catataaagc
     3001 aaccatttca gcagcagtta actcttttcc ttctcctgct aaaacgtatt ttttagtttc
     3061 tttgttgtac atttctgtag cagcaacgtc aagacctaac ataacgtctt cacctggctt
     3121 atatccagct tttgttatag cttcaactat taattctaaa gcttctctgt ttgaacctaa
     3181 gtttggagca aatccacctt cgtcacctac accacaagct aatccttttt ctcctaaaac
     3241 tttctttaac gaatggaata cttctgctcc cattcttaaa ccttctttga aacaacaagc
     3301 tcctactggt aatatcataa attcttgaac atcaacgttg ttatcagcat gctctccacc
     3361 gtttaatatg ttcatcattg gaacaggtaa ttgtttagca tttactccac ctaggtattg
     3421 gaataatggt aaacctattt catcagcagc tgctctagct actgccattg aaacacctaa
     3481 tatagcatta gctcctaatt tacctttgtt tggagttcca tctaattcta tcatgattgc
     3541 atctatagct ggttggtcaa atgcatccat tccttctatt tcaggtgcta ttatttcatt
     3601 tacgttagca acagctgttt ctacaccttt tcctaagtat cttcccttat ctccatctct
     3661 taattcaaca gcttcaaaag ctcctgtaga tgctcctgat ggaactattg ctcttcccat
     3721 tgctccatct tctaatacta cttcaacttc tacagttgga tttcctcttg agtctaatac
     3781 ttctctagca tatactaatt caataactga catttgtata tctcccttcg attttctatt
     3841 agttaaattt tcatttactg taaacatccg tctactattt taatctactt tctatattat
     3901 agtacataaa gttgcttttg aaaattaggt acaaatttat ttgatgggta aatttgtaaa
     3961 agttcctatt tttattttat tttttataat attttaattt atataatata aaaaaacttg
     4021 tctaaaatcc aaaaccaatc ttaaatacac aa
//
LOCUS       DAMJZE010000248         4051 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJZE010000248 DAMJZE010000000
VERSION     DAMJZE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.252"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            100..1269
                     /locus_tag="PC541_003609"
     CDS             100..1269
                     /locus_tag="PC541_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2841028.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            1452..1652
                     /locus_tag="PC541_003610"
     CDS             1452..1652
                     /locus_tag="PC541_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2841029.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            2073..2501
                     /locus_tag="PC541_003611"
     CDS             2073..2501
                     /locus_tag="PC541_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2841030.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(2644..3399)
                     /locus_tag="PC541_003612"
     CDS             complement(2644..3399)
                     /locus_tag="PC541_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2841031.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT         1606 a          462 c          614 g         1369 t
ORIGIN      
        1 tttttaatat tggacataat aaaatagaga attattaaat atgaaaatta ctatatgtga
       61 cagaatattc tttgtaattt gatttgtaag gtgtactatt tgggaggtgt agctttgaaa
      121 atactgaaaa ataagaataa gtacatcaaa ggtataatgg catttgtatt tttgtcttta
      181 gctactacag caggtgtatt tttaatcaat gatttaagta tgaaaaatat ggaagaaaaa
      241 gtcatggaat ctgcaaatgt tgaaggtgat gatgtaaaag accaatataa actattgctt
      301 gaaaacttat ttgactacag aaataaggca atattagaga aaaatgaaaa tattttaaaa
      361 ggtctttatg aaacggataa aaaatttggt ctgtgggctt atgaaaatga agtaaaaaag
      421 atgaaatatt tagaaaactg gtctagtaaa caaggtgtaa aatttaaaga cataaagaca
      481 agggttaaga ttaaaaaaat aaaggaaaaa gaaaaagacc tctatggtat aatctgttca
      541 gtatccactg aatataagta ttcatatgaa aatcaaaagg acaaagaaaa tgcctttaga
      601 ataggtacat atcattatct aaatgtaaaa attatagata atcaatatgt cataactaaa
      661 gagtggtata cagacccttt tgcagactct ttaaatttag aaaatataaa atctgatgat
      721 ataagaaatt atattggtca acaagatggt gtagagttac agcttacaga tgaacaaaaa
      781 aaagctatag attatgcaca taaatactgt ggagccgcag ctgatgaaga acatggtatg
      841 aaatttaatc caaactatag ggattataat ccagaaggtg gagattgtgc aaactttgca
      901 tcacagataa tgtttgaaag tggaaggttc aaaaagaact caatttggaa ttatagcgag
      961 agagcaggta caaaagcatg ggtaaatgca caagctttta aaaattatgc actgtatagt
     1021 ggaagaggtt cattaattgc taaaggttcc tatgaagaag tatacaaaga agcttataat
     1081 ttacgtccag gggattttgt tggttatgag aagaaaggca gaataacaca tgtttcgaca
     1141 gtgacaggat tagactcaag aggatatcct ttggtaactt gccataatac tgatagaatg
     1201 ttggtgccat gggatttagg ctggagtgat aagtccataa gattccatat aataaaaata
     1261 aattattaaa aaattataaa gaaaaataaa gtgtaaaaga taataatcat ttaaaattat
     1321 aaagtaaaaa aattgattat taatgtataa aaataataat aattgacaaa catagctata
     1381 tatgatagag tagtaataag gttacagaaa agagtagcta aatatttgtg tttttttagg
     1441 aggatttatt aatgaaaact ggtgtagtta aatggtttaa caacgaaaaa ggatttggat
     1501 tcatatctgt agaaggtgaa ggagatgttt tcgtacattt ctcagctata actggagatg
     1561 gatacaaaac attagaagaa ggacaaagcg ttgagttcga agtagtagat ggagctaaag
     1621 gtcctcaagc tgctaacgta gttagattat aattaaaaat tcatatatat tagtgttcta
     1681 gtaatatgag agataagccg ctaatcattt agcggttttt tcagtttttg taaataaaat
     1741 taatagcaaa ataaattaaa attattaaaa taaaatcatt aataatgaga taaatatgtt
     1801 aaaattaata gtgtagagta aaattattat ggctaagttt aagtcaatta atttttagta
     1861 aattttctaa aaagagaata agtgatactt atagaccata taaaaatatt aataaagcat
     1921 aataagatta aaatgagcaa aggaaaaacg cttgttacag cgtatagtat taagaatact
     1981 tagaaaataa gaaaggagaa agttactgca aaaaagtaac attataaata ttgagtaata
     2041 tttataagtt ttcagtaacg gtgggtagtg taatgaacat attattcttt ctaacaccaa
     2101 aaagtgaagt agcatatata tatgaagact acactataag gcaagcccta gaaaaaatgg
     2161 aatatcacaa atactctgca attccaataa taagtaagga tggtaaatat gttggaacta
     2221 taacagaagg tgatttttta tggacattaa aaaatgattt aaatctggac ttaaagggat
     2281 tggaagatgt tccagtgaca gatataaata gaaaaatgga taattctcct gtttccatca
     2341 acgcagatat tgaagattta gttataaaat ctttaaatca aaatttcatt cctgttatag
     2401 atgaccaaga cacatttatt gggataataa aaagaagaga tgtaataggt tactgttatg
     2461 agataatacg tggatataaa aatttagcta atgataatta aaaatcttat ttaaactttt
     2521 aagctgagtt ctttacaata aaaaaatgat aatatgaaac tgaatattta aactttaata
     2581 taaaaagctg tcttttctaa taaatattgt gaaaagacag cttttttata tatgacctta
     2641 tgattaatcg ttgtatttta caaggtttcc gtacttagca ttaagagcat ttatcttatt
     2701 tccataaact tcattttgtt tttgatataa taaacttttt tcaacttcag ccttaacttc
     2761 atcaaatgta gactctgtac tttcttgaag gtcttctaat tttattatat ggtatccaaa
     2821 ttgagtttta actggttcac ttacttcacc tttagccata gagaacacag cttcttcaaa
     2881 ttcaggaacc atttgtcctc taccgaatgt tcctaaatca ccacccatat ctttagatgg
     2941 gcaagaagaa tgtttaagag ctgcatcttc aaaagatatt tcttctgatt ttatttgagc
     3001 aagtatttct ttagcttttt catcactatc tactaatata tgtttagcag ttgcactttc
     3061 tggtttagag aaagagctct tattagcttc aaagaatttt actttttcat cttctgttac
     3121 attaacacta gataaaactt tatttatagc aaattgcttt aatacatttt cttctatctt
     3181 tttcatttct aatttaaata tttcttcttc atctaatttt tcttctttag catctaagaa
     3241 gaataattct tgattcacta aatcatttaa taagtgtttt tttccttctt cagttttaaa
     3301 ttgcatagct tgataagggt ctaatgattt taatgcattt tcaatatcaa tattagttat
     3361 ctctttttct ccaactgttg ctaaaacttt tttttccata atagttaact ccttcatagt
     3421 gtatttcatt attaatataa gactaccatc ttaaataata tcagaaaata atgtagatgt
     3481 ctaatattta aagccatatt aagtcaaata gaattaaatt tagtagaaca aagttattac
     3541 aattaataaa agtgagctca taatacccac tacaccttcg tcatctttaa atttaagagt
     3601 ttttactatt gaataataaa ggtatattcc agtaaaaata atctttaaat acaagaaaaa
     3661 taaactcttt gggtatatta aaaagctaaa aagtgtaaat ataaaagcaa gtagagtact
     3721 tatcctgtaa aagaaatcta tttgtgtaat gtctttatat tttatcataa acaccgaata
     3781 taaaatgatg aaaacaattg cgacgattgc atatatggta aataacatat tttcaagagt
     3841 accatttgaa attactatcc acttatacca cataattggc caaaataata gtgctaagaa
     3901 cttaaaataa ttatcaaaca acttttcttt atacattcat caaacaacct ttcttaacaa
     3961 aagcatatat ttgtttttag aattttaaat aatatgatat cattattata tattaatatt
     4021 gaatttatag aaaccaaaat ttgttaaaat a
//
LOCUS       DAMJZE010000249         4027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJZE010000249 DAMJZE010000000
VERSION     DAMJZE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.253"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(461..1780)
                     /locus_tag="PC541_003613"
     CDS             complement(461..1780)
                     /locus_tag="PC541_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2841032.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1774..1902)
                     /locus_tag="PC541_003614"
     CDS             complement(1774..1902)
                     /locus_tag="PC541_003614"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841033.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(2041..2751)
                     /locus_tag="PC541_003615"
     CDS             complement(2041..2751)
                     /locus_tag="PC541_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2841034.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
     gene            complement(3842..4012)
                     /locus_tag="PC541_003616"
     CDS             complement(3842..4012)
                     /locus_tag="PC541_003616"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841035.1"
                     /translation="MPPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTTVSSLL
                     GRIAVNIFNFNK"
BASE COUNT         1500 a          571 c          304 g         1652 t
ORIGIN      
        1 agtcctcctc aaattagtaa aatatttttc tttaatattt attactaata tcaactttac
       61 tatctaatta gttatttaat tgttgttttt atgttcctag ttataaagtt tatcaaattt
      121 acattttatt tttttatact atatagaact tttggtcaat aatgttacca tactaaggtc
      181 gcatttgcag ataaaaataa ctctattttt ctaaatttat ttaagatata tcatgcaaat
      241 aatataacta taaaacctat caacatttta aattcaaaca aatataaatc acattttttg
      301 ttctattatt ttattgagta ctaaaaagga tgttatctta ttagcatagc attcaaccat
      361 ttttatcacc tccaaatatc ctatgcctta aagatagcat cttctatttt atctttcaat
      421 actactgctc caactgggca atttagagcc ccaactagaa ttagttccta gttaatggta
      481 tataaatagt aattgtaaac ttgtttttat ctgagtatat ttccacattt ccattatatt
      541 tttctactga actttttaca ctacttattc ctattccatg taaaaaggaa tctttcttac
      601 ttgtaataac cttattattt tttaatacaa cttcatttat ctttgtattt tcacatttta
      661 ttacaaataa cctatttata acagttcctc ttaatttaat ctttttttgt atacttgtgt
      721 ccttaatctt aatacaagct tctattgcat tatctattat atttgaaaat atactacata
      781 tatctggcat ctctataaaa ttacattcta aaaaattaat atctactaga aaatatatat
      841 tattcatatc acaaatagat tttttatcac ttaaaataac atctagaatc atattctgag
      901 tattaaatat agtattacat tcttttattt gcttatttat atctttaata tacttatctg
      961 caaactcatt tttcccataa acattctgaa tacaaatgat atgattattc atatcatgat
     1021 atagttttct tattttcaac tgatattcct gtaaatttaa ataatattta tattgcatat
     1081 tcattctttt ttttgtaatt tcatattcta tctttaaact attatctttt attattcttc
     1141 ctattatgct tactaatgaa agatttgata gtaaaaatac agttgatatt gtaagtatta
     1201 ttaaactctc tttagaatcg ataatcttat ctttaaaaat aaattcaaat attactatta
     1261 caccaataat gtttgctatt aatggaatag ataaatatat acaatccctc ttgtttattt
     1321 ttgtttttaa tctgataaac ttaagcaatg gtattaatac tattagtaat gatttagata
     1381 tcattattaa ctctaacttc aacaaattat cctctaataa atcattcata ttttttatag
     1441 aattaagtgt cattactata cttaatccaa tactatcaaa tccaattaat aacatccaat
     1501 atagcaaact tataaataca cttttccatt tatccactac ataatttatt ttatacaata
     1561 catatgtaat aaatatacat ataaataatt taatatttat attaaactca attgtattca
     1621 acatagtaat tatcataata gcaattacta tgctactatt tagggttaat ctattctttc
     1681 ttaactcact aaattcattt aataaaaatt ttaatacaat ccattctatc gccgtagaaa
     1741 atataggtaa aaaaatccaa aatacattta tatttaacat aatacatctc ccaatacata
     1801 tgttaatttt ttctttaaat tagccattct atatttacta actggtatat ctttaccatt
     1861 aataactaca gtatttttac atataaattg aatatactcc aaattaatta aatagctctt
     1921 gtaacatttt taaaaattta ttctttctta aatcactgaa ttcatttgat aaaaatttta
     1981 atacaattca ttctatcgcc gtagaaaata taggtaaaaa aatccaaaat acatttatat
     2041 ctaacataat acatctccca atacatatgt taattttttc tttaaatcag ccattctata
     2101 tttactaact ggtacatctt catcattaat aaccacagtc tttttacata taaattgaat
     2161 atactccaaa ttaattaaat agctcttatg acatctaaaa aaattatatt tttctagctc
     2221 taattctatt ttatccatac tatttttagt ataataagta tcatttttag tatgtatagt
     2281 caaaaccttt ttcctaacct ctatatacat aatactatta attaaaattt tattgattat
     2341 acctttattt tctatcagca taaaattttc tctttttttt ataatatccg atatacaact
     2401 caaaatactt tcatttaaat ctccaaaatc tataggcttt aataaatatc tataagccct
     2461 taccttatag ccttcctgta tgtaatcaac tattgaagtt ataaatatta tttcagaagt
     2521 atcattagtt tctctaatta ttttagccac atccatgcct gttagttgaa gcattttaat
     2581 atctaaaaaa aatatatcaa taccttctaa atttgtttcc aataattctt ctcctgaatt
     2641 aaactcaaat agttcaattt gactagaaat accctctaag gactttaata tacactctct
     2701 taaaaaagct atttgtgtaa tatcatcctc acagataact attctataca taaacacccc
     2761 tccttagaca ttagatctga aaacaaataa aatatcaatt tataaataat tatttagtta
     2821 tacaactaac atggtcatca aatactatta ttcacttaaa aaatcacccc ctatttttag
     2881 tatgttaaca acaaaataag aagatatctt agatttttaa aaacaattaa tacttaatgt
     2941 agttttgtat actatttata ggtaattttt tatatcattt taaacattaa aattattgat
     3001 gtttaagaac tattatattg atagtaatat ataattaatg atatcaaaaa tttttaatta
     3061 gacttctctc ttattcaaga tttttctata taaaatctaa gaataaatag aaaactaaca
     3121 tctaaccttt tattttttat tttcgatcta tttacttgaa ttttaacata aatctatctt
     3181 ttagtatata ttaatcatgc tgaatttgtg atgacatgta tacaaaatat cgtttagtat
     3241 tttctaataa aaaatattac tgaaattaaa aatctacaat agcatttaac aagtaaaaac
     3301 atgtttacac aaatatataa tttaaggtct ttaaaataga gtatgtatta ttctaaaaat
     3361 tagttatagt tatcacataa aaatagaaaa gaattttcaa aagatttaaa gaacatatat
     3421 acttcaattg cagaagatgt aatgttaagt gaactagcat tcttcgagga aaaatagact
     3481 caaaaaatta gaaaatagaa tcaggtttta gatgatttaa gtatttattt tcaaaaaaga
     3541 aatttgattc taagttaata aagaaatatt taaaaaaatc aaaaaatttt cttaaataag
     3601 taataggttt tgtaaaaatg gaaaaatatc aaaataagac tatgattttt aaaactatag
     3661 ccttatcaat aaatttttat ttaaagaaca cacataaata tttttaaacc taatttatgt
     3721 gaagttttat gtatttttta tataagtagt attctaatct tactcatcta atataccaaa
     3781 aagactgtaa gtaagttcac ttttatgcaa acttacccac aatctctttt acacattaat
     3841 tctatttatt aaaattaaaa atatttactg ctattctacc caataaagat gatactgttg
     3901 ttgttctggc tggtactgtt acttctattt ctcctgtatc tgctgtttta ccacttgatg
     3961 tgtgtgtcac tgtaaatact aatgttactt ttgtttctgt atctggtggc actattgttc
     4021 catcttt
//
LOCUS       DAMJZE010000250         4008 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJZE010000250 DAMJZE010000000
VERSION     DAMJZE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.254"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            49..717
                     /locus_tag="PC541_003617"
     CDS             49..717
                     /locus_tag="PC541_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841036.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            876..1382
                     /locus_tag="PC541_003618"
     CDS             876..1382
                     /locus_tag="PC541_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2841037.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1510..2526
                     /locus_tag="PC541_003619"
     CDS             1510..2526
                     /locus_tag="PC541_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2841038.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2880..3584
                     /locus_tag="PC541_003620"
     CDS             2880..3584
                     /locus_tag="PC541_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2841039.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3775..>4008
                     /locus_tag="PC541_003621"
     CDS             3775..>4008
                     /locus_tag="PC541_003621"
                     /inference="COORDINATES: protein motif:HMM:NF022521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 family protein"
                     /protein_id="HDF2841040.1"
                     /translation="MVIGLLSGFYYRELTKAHDFVGDTQLSLVHTHTLILGMFMFLLL
                     LPLEKVFKLSSYYLFNWFFFVYHLGVLITISMMT"
BASE COUNT         1680 a          353 c          698 g         1277 t
ORIGIN      
        1 tatattatat taggtatttt aaataaattt tttaaagggt gattttttat ggaaaatata
       61 actagttatt ttacaacaat actatgtgta tttatatgct tatcatctgt atttatattt
      121 actcaactag ctagagtatt tattaataag aagaagatta atcaaaaaat taaatcaaga
      181 aacggattta ggtatgatag agattttatt gaagccagaa gagaagaaat acatataaag
      241 gataacaata ataataaaaa taagagtaac aataagaaat taaagggaga aaaagtattt
      301 aaatacgata atggtgattt atataaaggt gagtttgttg atggaaagaa aaatggattt
      361 gggatatata ttttttcaag taaggaaaag tatgaaggat tatggaaaga tgacaagatg
      421 catggaatag gaaaatatac ttatagagat ggttcaatat atacaggtga atttaaatac
      481 ggattaaaaa atggtttggg aaaattaaca tatcccaata atgacattta taagggatat
      541 tttttagata ataagaaaaa tggtaagggt gtattatata aaaatgatgg aaataaacaa
      601 gcaggaatat gggaagatga tgaacaatgt aaaagtttag attttaaaga tttaaataat
      661 aataaaataa ataatgttta tcaaaataga ttaaataaca ataaattaaa agtttaatta
      721 ttctaataaa aaaatcttta tttatgatgg aatataggat atatctatgt tgtaatttgt
      781 aaaatcttcc tatatcatat aaaattaagt attgattagt ctattacata aatatagaat
      841 ataaatatat ccatttaata ataaggagaa gagatatgaa agagtatgta gttgtggatt
      901 tagaaactac aggattagat ccatataaag gttgtgaaat aatagaaata ggaataacag
      961 aaataaaaaa tgaacaaatt gtaaaaaact attcaaggtt gataaaacct aaggggataa
     1021 tttcttcatt tataacagaa ttgacacata taagtaatga aatggtagaa aatgaagaac
     1081 cactagaatt agttttacct agatttagaa aatacatagg tgatagaact ataattgcac
     1141 acaatgcgaa atttgattta aaatttttaa attactatct tagaatgtta gatttagaac
     1201 caataaacaa ttatatatgt acagtagagt tattaaaaaa atgtaaaagt tataaaggaa
     1261 aaaacaagaa acttgaaaca gcttgtgctt attacaacat agagaatata aatgctcata
     1321 gagctgacag tgatacatta gcaactgcaa aactattttt gaaaataaaa gatgaatatt
     1381 aattataaat atagagaaaa ttagtgaaaa tatcctttat aaaattgacc cttaagctta
     1441 caattttgtt ctttgattta ttttgtagaa caatgtataa tgtaccttat atgaagaagg
     1501 gagactaaaa tgggttatta caatgataat gataataata aaataagacg taaaaaaatt
     1561 agtaacagca aagatagtaa taaaaaatct aaacattctt atactccggc aaataaaaaa
     1621 aataaaagtt ctaagacaag ttctagtaaa agaatgagtg aaaaagtaga ttcacagaat
     1681 cttataaaaa gagatagaga tagttttaca agagataatg taagaccttt aaatacagat
     1741 tacaatcaaa gagaaagaag aagagtcgag tataaacaaa gagagaaaaa attaaaaagt
     1801 aaaaaaagaa aaaaacaaaa aaggattaga atagttagag cattattatc tattatgtta
     1861 gttgcactaa ttatattttc agtggcaggt gcaatattta cagtttcagc tataaaaggg
     1921 tcaccagaag ttactaaaaa gttaattagg tctaaatata taagtagtga ggttgtaagt
     1981 ataaaacaaa tgccaaatga tttaaaacat gcaatagttt ccatagaaga tgagagattt
     2041 tataagcata aaggagtaga tgttatttct ctagttaggt cagtattaaa caatgtgttt
     2101 acagatacaa cacaaggtgc aagtactata gatatgcaag tttcaaaaaa cctgcttact
     2161 agtaatgata aaactatgaa gcggaaaata agggatatgt ataatgctaa gcagatgaat
     2221 aagattatga ctaaggatga aattcttgaa gcatatttaa ataatatgta tctaggtaaa
     2281 aatgcatatg gagctgcaaa gggagctgaa gtctactttg gtaaaaaagt tagtgaatta
     2341 aatttagctg agtgtgctat gttagctggt atgacaaata atcctgcaag gtatattgac
     2401 catggagagg ctaagaagag acaagtgatt gtactgtata agatgcatca gcttggttat
     2461 ataactgata aggagtatag aatagcaaaa gctgatgaca ctccatttaa atcagaaatt
     2521 gattaaaatt gtaagaatat agtattttca atagtttaaa caaacaaaaa tgtaataaaa
     2581 aaggtgattt gccacccatt tgccaccata attataatct aatggcaaat tgttttaatg
     2641 tacaaactag ataatagaat attgtcttaa atgctatatt atgctaaaat ttaattgtat
     2701 tttgtaaatt aaaaaactta taatttagtt ttctagggtt ccgcatttta taatggtcag
     2761 gtccaagaga aaacgcgcaa ttgttgtgta cacggaagga taaaagcctg ggagatattt
     2821 aagaatattt ctataggctt ttttattttt acatatttta aatttgagga ggttttctaa
     2881 tgaaaaaatt tgttattgag gatgcatttt ggaatctatt tcctagtgca aaaattggag
     2941 ttgttatttg taatgatata gataactcta taaaagatga agactataag aaaataattt
     3001 tgaagggaga agaagaagca ttaaaatact tggaggactc agaatttagc aacaaccaag
     3061 tgattaaagt atggagagaa gcatttaaaa aatttaaaac gaaaaaagga gcaaggtctt
     3121 ctattgaagc attattaaaa agagttcata atggaaataa tataggaact attaaccctt
     3181 tagtggatat ctacaatttt atctcattaa aatatgcatt accttgtgga ggggaggata
     3241 tagataaatt tataggagat attaggctaa ctagagcaat tggtaatgag gagtttatcc
     3301 cactagggac agatgaaaat tcatttccat atgaaggaga aataatttat aaagatgatg
     3361 gtggagcaat ttgtagatgt tggaattggc gtgaagcagt gagaacaatg cttactgaag
     3421 atactaacaa tgccttttta tgcattgaat tagtagatga aagtagattt aaagaatttg
     3481 aaaatgcttt aaatgaatta gctaaaatag ttcaagaaaa attgggtggg aattgtaaaa
     3541 tttcaatttt agatattgat aataaagagg tatcagtgga ttaatatgga ggtgctgttg
     3601 taatggacta ttctagttat aaagatactt atatttaggg taagaaggtc tattttagat
     3661 ataacgatta tcaaaggata taatatagaa aataaaaatg tagaatataa aaagccttta
     3721 aaataagaaa aaaatgtatt ttaaaggctt ttattatact cttttttatt ttatatggtc
     3781 ataggtttat taagtggctt ttactacaga gaattaacta aagcgcatga ctttgtgggt
     3841 gacacgcaat tgtctttagt gcatacacat acacttatct taggcatgtt tatgttttta
     3901 ctcttattac cacttgaaaa agtatttaaa ttaagtagtt attacttatt taattggttc
     3961 tttttcgtgt atcatttagg tgtgttaatc acgatttcaa tgatgaca
//
LOCUS       DAMJZE010000251         3991 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJZE010000251 DAMJZE010000000
VERSION     DAMJZE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.255"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(238..618)
                     /locus_tag="PC541_003622"
     CDS             complement(238..618)
                     /locus_tag="PC541_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2841041.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(819..2012)
                     /locus_tag="PC541_003623"
     CDS             complement(819..2012)
                     /locus_tag="PC541_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2841042.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2075..3331)
                     /locus_tag="PC541_003624"
     CDS             complement(2075..3331)
                     /locus_tag="PC541_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2841043.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1449 a          734 c          415 g         1393 t
ORIGIN      
        1 aaaactttat ataaagaata gaaacaagtt ctagattact atagagatga catatcaaaa
       61 tatttttaaa aagtaattta agtattagaa gtaattgaag ttagtaaata taaatgcgtt
      121 tacttttaag tattgcttaa gggaataaaa aataaaaata aactctgcaa ccatctaaaa
      181 atagttgcag agcttacaaa accaaaatta tctaaattgt aataaaaata ataacattta
      241 tacataagct tcttcccaat ttactaccca gttttctaac gactttatct tttcctctgt
      301 tagctcctct tcgtgaaatg atactaataa attccttata aaattattct tcaaaagatt
      361 ttgcacattt cttgatataa actctaaata tgccttttct tttattgcaa ctgtataata
      421 catacacttg cctgtttctt gaacatataa gaatcttttc tttgatagtt ttgataaagt
      481 ttttaaagct actttttctg gtaaattatg ttcttctttc atataatcaa taatctcata
      541 tgaattggtt tttatatcta aattccaaat aaatttcata ataactaact ctaattttga
      601 taattttttt aaacacatag ttatcactcc gcttctttat ctatataatt aaatattaca
      661 ataataattt cacaaaatca attctaataa tcataacata ccttaaaatc tgttcttaac
      721 ttctaccttc ttaatctccc aaatgatttt tttatattgt attcaatagt ataatccttt
      781 tccaaaatat aacttaccag ttaaaataat ctttattttt acttactctc gccttttaca
      841 gaagttaata attttttata agttacatca attccaaaag ctccaagagg tgcagttata
      901 agtatagcta gaactgctaa cgtcaaaata agttgtccag atgccatccc cattgcaagt
      961 ggtacacctc ctattgctgc ctgtactgta gcctttggaa tatagccaat catacagaac
     1021 aacctttcct ttattgtcaa atttgtctta ataaggcaga agaatactcc taccatacgg
     1081 aataaaagaa ctccaaatat taaaattaca gctccaattc ctgctttaaa tgcataactt
     1141 atatctactg ttgcccctac caaaacaaac aagagaattt cagcgccaac ccatagtttt
     1201 gaatacttta tggacaatct tcttgcaact acacgatatt ttatctgaag gaaaatccca
     1261 atactcataa ctgctaataa tccagaaaat ggcacaatac cctctaaagc agtctctaaa
     1321 cttatcatta aaaatgagat acttaaaagc aacacccctt tagcagaatc tctcatatga
     1381 accttcttaa aaaatactgc taaacagatt ccaatcactg ctccaactat tacacctaat
     1441 ataattgata ctggaatttg taaaaaacta atagctgata tactatttcc ttgtgccaat
     1501 cctgtgaata ctgtaaacat cacaattacg aaaatatcgt ctatagaagt tcctgctaaa
     1561 agcatctgtg gaatactttt ttctgtacca taaccttctt ccattaattt aagcatttta
     1621 ggtacaataa ttgcgggaga aactgctcct actactgcac ccatgactgc tgcttccaat
     1681 atagaaacgc ctagtaattt tggtgctaaa actatcattc cgataatttc aaaagtagct
     1741 ggaataaagc acataagtac agcaggtctt cccactttct tcaaatcgtt tatatctaaa
     1801 gataatccag cccttgttaa tataataatc aatgcaatct ttctaaggtc agaagaaata
     1861 tctagtattg aattatcaat caaatttaaa acatatggac ctaatataat tcctgtaagt
     1921 aacattccca ataagttagg taactttatc ctcttaaaaa tacctcccga ggccattcct
     1981 aataaaaaaa tcaaagctaa acttgtcaac atattcctac cctatccttt ctctaatatt
     2041 ctttctcttc taatcataca ttatgttgtt ttatttattg ttttgttgaa attttttgta
     2101 gtgaataaat agatttatcc atattaaata tatatgaaat agcgcatact acaaagaaat
     2161 aaggtaaata gctgtatcca aaaacttctg cccctataaa tacaggtgca aaaaaagtat
     2221 ttgtagcact accaaagaca ctagcatagc caagcgctgc aactaattca attggcaaat
     2281 taaaaaatcc tgctaacaat acaccaagac tagctccaat ggaaaacaaa ggtgtaacct
     2341 ctcctccttg aaaacctgct gacaaagtca gtattgtaag tataaacttt aacagccaat
     2401 caaatgaata aatttctcca ccatagaaac tattttgaat taaatttgtc cctaaacctg
     2461 aatatcttcc tttataaaat aaaaggaata atacactaag acatactcca ataatagcaa
     2521 ttcttatcat aggatttttt aatctattac ctatttttct ttttgacaat ttcagacacc
     2581 atgcaaatgc tccaccaacc attccaaaaa taattcctaa aacaataagc ttccaaaata
     2641 ttgacaagtc aaatacaact ttagaagaaa gtgcaaaaga aaatttctct aaccctaaag
     2701 cttttgatac tgaacttgct gtaaaagaag cagtaaacgc aggaaaaagc gattgatact
     2761 ccagtgaacc agctaccaaa acctccatag caaatagtat tgctgctatt ggtgtctcaa
     2821 ataatcctgc aaaaccagct gccataccag ttactagaaa aatactacaa gcatttttta
     2881 taggcaacct tttacctacc caatgtgaaa aagttgcacc aatctgaaca gcaactcctt
     2941 ctcgtcctgc actaccacca aataaatggg ttaaccatgt acctgatatt ataaatggaa
     3001 ccaatcttaa aggaataatt tcttcttctc catgccctac ctcaaatatt agattcattc
     3061 ctttactact ttttcctcca aatttaaaat agcaataagc aataacaact cctactactg
     3121 ctaaaaatgg tatcagatac attggatatg tttctcgaat atctgtaact tttagaagaa
     3181 ccgttccaaa tatggtgtct ataagaccta taattgcacc aactggtata cctatgagtc
     3241 ctaaaaaaaa tagtccacca tatgttttat aaacgctttt aattcttatt atcattttaa
     3301 tccatattaa agttttaaat ttttctatca ttttaattcc tttctatttg catcataaga
     3361 ttttttgaaa gttttattac atcaactgta gtcaatgtaa tgactatcat aattgttttt
     3421 taaattgata ggatataggt acactcttaa cccattctta ataaaataat taacatataa
     3481 tctactattt tttctattaa ttaaatattt cttcaaattt aaaaaataaa aatttagtaa
     3541 gtacttcacc tccattttat tgatttaaac aacaaaaaaa gctggtggca tctatgatat
     3601 catagaagtc atcagctcat tggcagttta ggatattatc atccaaggga gaacctcatt
     3661 cccataatta ttatataatt ttttattttt catatgtcta ttttatgtaa attgtatttt
     3721 taatgttata aataatataa cctatcaatt aaaatagtaa agcctctaat attatgagtg
     3781 ttcaatattt catattattt ttatgatata taatttaaat gattttcttt actttgcatt
     3841 atgatacata acttaaatat ttttcctcac tccacattat gatatattat ttaaatattt
     3901 ttcttcactc tgcttatatt atgatacatg atttaaatat tttcttcact ctgcttatat
     3961 tatgatacat gatttaaata ttttcttcac t
//
LOCUS       DAMJZE010000252         3953 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJZE010000252 DAMJZE010000000
VERSION     DAMJZE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.256"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(68..928)
                     /locus_tag="PC541_003625"
     CDS             complement(68..928)
                     /locus_tag="PC541_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2841044.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1077..1967
                     /locus_tag="PC541_003626"
     CDS             1077..1967
                     /locus_tag="PC541_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2841045.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            2185..3939
                     /locus_tag="PC541_003627"
     CDS             2185..3939
                     /locus_tag="PC541_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2841046.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1609 a          422 c          626 g         1296 t
ORIGIN      
        1 aagtataata taacatataa aaagttgaat ttgtatatgt taattatact tgctatttat
       61 ttgttagtta ttctattttt ttaaatccat tacctaaagc ttcatgtata tctgtaatta
      121 taataaaagc attagggtcg acatttttta ctaatttttt caattcgaca acttctttct
      181 tttcaactac tactaaaagt acatttttat tttctccagt atatgcacct tctcctttta
      241 gaatagttgc acctctatct aaatcttcca ttattttttt acttatatct tttgaaaggt
      301 cagatataat agtgaatgat ttggaataat taaatccttc aataagcaag tcactaactt
      361 ttactaaaat gtataaagcc acagttgaat aaagtgcaat ttcaacctgt ctatttacta
      421 tacctgataa aacaactaca acgcagtcta caatacccat taaaacaggt atactaaatg
      481 taggaaaata tttatttagt aggagtgcca ttaaatcagt tccacctgta gagccattta
      541 ttctaaaaat aagtccttga ccaactccca ttaaaataga accagatata attgcaagat
      601 acatatcatt tgttatatct aatgtagcca tatttgcagt aagcttaagt gctatggtaa
      661 gaaaaattat tcctaaaacc gttttaaaac aatctttttt tgaaagtatt ttgtaagcaa
      721 atatgaataa tggaacattt aaaagtaaat tgacaatcca aagttctaca ccgaataatg
      781 aatttaaaac aactccaagt cctgttatac caccagcagc gattgcatgt ggattaaaaa
      841 ataagttaag agaaatggcc attaatatac agcctacaat taatattaaa atttctgaaa
      901 aagttattct atcttttttc aataacatca ctccttatat atagattata atatatatta
      961 tatagttttt ccaattttta ataaaagaaa taaaaatata ttataaaaac aatttgattg
     1021 gaattttcag aaaattaatg ctataattaa aagtaaattt aaagggaggt tataaaatgg
     1081 ctaaattaac tctagaacat ataaaaggaa atacatatta tattccactc cctacaatag
     1141 taggtgtata cgtagatggt aaggatgcaa ttttaattga cagtggaaat aataaggata
     1201 cagctagaca agttttaaga ttattagaag aacataattt aaatcctaaa ttaataataa
     1261 acactcattc aaatgcagac catataggag gaaatgcata tttgaagaat caaacaaaat
     1321 gcaagattgc tacgactaaa attgaaggat attttacaga aaatccaata ttagaatcag
     1381 catttttata tggtggctat ccaagtaaag cactgaagaa taaattttta ttagcaaagg
     1441 aaagtgaagt tgattatatt atcccttcaa atggcaaaat tgtagataca gaacttgaag
     1501 cgatttctct tccaggtcat tattttgaaa tgattggtgt tagaactcca gataatgttt
     1561 tgtttgttgc agatagtctt acaccagaga atataattac taaatatcat tttttcttcc
     1621 tactggatat agattcacag tttaagacac tagaaaaatt gagaatgtta gaggctgatt
     1681 tctttgttcc aagtcactct gtcaaaacta cagatataaa aagtttaatt aatattaata
     1741 agaagaagat ggaagaaata atagataata ttaaaaaggt ttgttatgag ccagttatga
     1801 ttgatgaagt tattgaaaaa atgtgtgatt tgtacaatgt caagcttgat gctaatcaat
     1861 atgtgttggt tggaagtaca atacgttctt atataactta tttatatgag aataatatgg
     1921 ttgaatatat atttgatggt ggtaagatga tgataaaagc tgtgtaaata gtaataattt
     1981 tcaaattata tgatgaaatt gttttagtat gactgttatt tgaaaagtaa tataaaaaaa
     2041 tttatataaa taagctaaga tgattgatat aaagttactg ataattttaa ggtaatatat
     2101 acgttactat aatattatgt tattatttgt aaataagggg ataatttata agcaaattaa
     2161 agaccttaat ttacaggagg aatagtgtat attatgaata aaaaattcaa aaaattcagt
     2221 aaaaaactaa gtatattaat tttagttttg attatgtttc tttttaataa tttaactgca
     2281 aatgcacaga ttaaagaaga ttcaataata ggaattgata gatatgaaac agcaggactt
     2341 atagcagata gacaaactta tgatactgta atattagtta atggagataa aaatttatct
     2401 gatgggcttt catcaagtgg attagctgga gctattaacg caccaatact tttgactaaa
     2461 aaaagtgaaa taccaaaagc tactttaaat agactagata ataagagttt aaatgttgta
     2521 aaaaaagtat atataattgg tggttataat actattgaaa attcagtaga aaaagatata
     2581 aaaagaaaag gtattcaagt ggaaagaata aatggtaaca atagaattga aactagttat
     2641 aatgtagcta aaaaaataaa tgaagtaagt agtgtaaaag aggtaatgct tacaaatgga
     2701 tttgtaggag aggctgatgc tatgagtata gcacctgtag ttgcaaaaaa taaaggagct
     2761 ataatactta cagatgggaa atcagttcca tttaagactg aaagtttaaa tgtttacgct
     2821 ataggtggca aatctgcgat aaatgaggat ttagtaaatg aaactaaagc aactagaatc
     2881 ggaggaaatg atagatttga gaccaataaa aaggtaatgg aaaagtttta taatggagcg
     2941 acagattttt atataacaaa tggatatcaa ttagtagatg cactaacatt atctccttta
     3001 gccaaagaaa aaccaatcgt tttagttgct gatggaagta ataaaggtat tttaaaagga
     3061 gctaaaagta taacaaaagt tggaggtatt gatgccaata tatataaaca gtgtttagat
     3121 atgattaaat ataataatat ggatatgact ccaaatattt taaaacactt aacttcagtt
     3181 gaaggaaatg aagtttctga aatacttata gaatcagata atttaggtgt ggttgttgaa
     3241 aagacgaata ccaataaatt tgagtttgat tacgtaagtg taactaatga aaaaaattgt
     3301 actttttctg tatataaaga aaattcttct aataatataa aaaatggaaa gctagttgta
     3361 agtgctaaga aaaaaataga taagaaagat agtgatttgc aagatatgaa tggcgacaat
     3421 atgataaatg caaataaaga taggatggta aatgtgataa aaattggagt tcccgataaa
     3481 atgtattcta attttaaagt agatgttgaa agtggttcag tagaacttta taatattaaa
     3541 ggaggagcta ctgttgatgt caatgacggg attgctaaca ttgttgataa caatgtaaca
     3601 aatcctttca atataaatac taatagtggg atttcaggtg taacagctga aactatatca
     3661 tctgaaataa aatttagagc aaacaatgga attgtaaatg taacagctac gaatataaat
     3721 ggcaatataa gtttatttgg aagcgataat aaaggtacct ttgatggaat atttaaatta
     3781 aatctgaaaa aagaaccatc aaatttacac ttgaagttaa ttggaaatgg gataaataaa
     3841 ttgccagaag gttggtctaa agattatatt ttaggaaatg gacaaccaat tattgaagtt
     3901 aaaaataatg gcataaataa tatatcaatt ggagaataaa aacatatgaa aat
//
LOCUS       DAMJZE010000253         3910 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJZE010000253 DAMJZE010000000
VERSION     DAMJZE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.257"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            111..1766
                     /gene="ilvD"
                     /locus_tag="PC541_003628"
     CDS             111..1766
                     /gene="ilvD"
                     /locus_tag="PC541_003628"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2841047.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2110..2739
                     /locus_tag="PC541_003629"
     CDS             2110..2739
                     /locus_tag="PC541_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2841048.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            2765..3577
                     /locus_tag="PC541_003630"
     CDS             2765..3577
                     /locus_tag="PC541_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2841049.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1513 a          445 c          734 g         1218 t
ORIGIN      
        1 ttttcattag tatatgctaa tattttttta caattttcgt aattaattaa tagaaaaata
       61 tattataata atattatgaa aatttaaaaa agtaagaaag aggtaatatt atgagaagtg
      121 atataaaaaa aggaatagaa ggagctccaa agagagcgtt aatgtatgga atgggtctta
      181 ctaaagaaga aattgaaaga ccattaatag gtatagtaaa tgcacaaaat gaggtgattc
      241 ctggtcatct gcatcttgat gaaatagcag aagctgcaaa aaatggagta agaatgagtg
      301 gtggtttgcc attagaattt cctgctatag gggtatgtga tggtatagct atggggcatg
      361 tcggaatgaa ttattccctg gcttcaagag aattaatagc agattcaata gaagctatgg
      421 ctatggcaca tggttttgat gctttggtac ttatacctaa ttgtgacaag atagtgccag
      481 gtatgttaat ggctgctgca agacttaata taccaagtat tgttgtgagt ggagggccta
      541 tgcttccagg taaaaaaaat ggaaaagtat atgattttaa ctctgcaatg gagggtgttg
      601 gagcatgtaa agatggaaca gtaagtgaag aagaattaga agagttagcc atgaactctt
      661 gtcctggatg tggttcatgt tctggtcttt tcacagcaaa tagcatgaat tgtttaacag
      721 aagctttagg catgggtata ccatataatg gtactgctgc gtcacattct ggagaaagaa
      781 aaaggatagc aaaatatgca ggtatgtatg ttatggagtt acttaagaac gacataaaac
      841 ctagagatat tttaacaata gatgctttta aaaatgctat agctgtggat atggcaatgg
      901 ctggttctac aaatacagta cttcacttac ctgcaatagc ttatgaatca ggaatagagc
      961 ttaacttaga tttttttgat gaaataagtg aaaaaactcc ttgtttaaca aaattaagtc
     1021 caagtggaaa acatcatatt gaagatttac atatggcagg aggaatacca gctataatga
     1081 acgaactttc aaagataaat ggaataaatt tagattgcaa aaccgtaaca ggcaagacta
     1141 taggggaaaa tataagaaat tgtgaaatag aaaatgaaga agtaatacat acattaaaga
     1201 atccatatag taaccaaggt gggcttgcaa tattgaaagg aaatcttgct ctaaatggag
     1261 ctgttgtaaa aaagtcagca gttgcagaag aaatgttagt tcatgaagga ccagcaagag
     1321 tttttaattc agaagaagaa gctgtaaatg ctatttttgg taaaaaaata aacaaaggtg
     1381 atgttatagt tataagatat gaaggtccaa agggtggtcc aggaatgaaa gaaatgctat
     1441 ctcctacatc agcagttgca ggaatgggac ttgataagca tgtagcactt cttactgatg
     1501 gtcgtttttc aggggcaact agaggggcat ccataggcca tatttctcca gaagctatgg
     1561 aaggtggttt aatcggacta gttgaagaag gagatataat ttctataaac ataccagaca
     1621 aaaaattaga gttaaaagta gatgaagttg aaatagaaaa tagaaaatta aaatttaaac
     1681 ctttagaacc gaaaataaag catggatact taagtagata tgctaaactg gtaacatcag
     1741 caaatacagg agcagtttta aaatagtaga ctatatttat gtaaattaga aaaagttaag
     1801 aaaatattaa ttggatatct taggtgttat agcactaatg aggtatcctt tttattttgt
     1861 atgaaaaaca tagcaaaatc ttgattatct ttgaaaacaa tacatctcta ttataaggaa
     1921 tacgagtaca gcatagagat gtatctttta gaccttgttg aggtaataca actttttaaa
     1981 agtttgatgt ggaatgcttt tcgttccttc tgaactatga agaaattgaa agtaagttaa
     2041 ttcttgacat aataaatatt aaaatgtata ataaaaaaca aacagacagt ctgtttttag
     2101 gagggaaata tgaatagaga agaaaaaagt aaaaatagta aagaaaaaat tatccaatca
     2161 gcattttcac tattttcgtc taaaggatat gattcaacat ctacacaaga tattatcaat
     2221 ttatctggtc tatctagagg tgcaatgtat catcacttta aaactaaaga agatatactg
     2281 agaagtgtca caaaagaact ttactcacaa atgaataatt ttttagagca tcttgttgct
     2341 gatgacaccc ttacagcaaa tgaaaaaata atagaattgg ttgttcatag tgcgaatgat
     2401 tacacacgta gaaaaatggt acattgtagc tggttagaaa aaatcccatt cgctttaata
     2461 gaggaagttc gtaatctcaa caatgtagtt gcacccaata ttgctaagat aattaaacaa
     2521 ggtgttgaaa ataaagaatt ttcttgtgaa tatccagagg aattagctga aatgctcgtt
     2581 tttagtattg atattttact tgaccctgta ttatttaaac gtgaatacag tgaagtatgt
     2641 aataggttgg attttttatt attcatgtta aaaaaaatgg atattcctct aattgatgag
     2701 tatggtattc aaaaatttaa agatttattt aaacaatgat aataggaaaa gtgaggaata
     2761 gactatgttt tataatgcaa aaagttgtca aatgatgata ggaagtaata cagtgaatta
     2821 tattgaattt ggtagtggta agaagacact aattatttta ccaggattag gagatggttt
     2881 atttccttta catggaaaaa tacaggcgat tgcttttgct tttagatata agcaatttac
     2941 aaaagattat aaggtttatg tatttagtag aaaaaatcaa attacagaaa aatattccac
     3001 aagggatatg gctaaagacc aagcagatat aatgaaaaaa cttggaatta tgaaagcaga
     3061 agtaatgggt gtttctcaag gtggaatgat agctcaatat ctggcaattg attatcctga
     3121 gttagttgag aaacttgtgt tggcagttac ttcttcaaag caaaatgata ctatccaaaa
     3181 tgttatttgt agttggattg atatggcgaa gaagcagaat tacaatgatt taatgattga
     3241 cacagcaaag aaatcctatt ctgaaagata tttaaaaaaa taccaattat ttattccttt
     3301 tttaggaaaa gtaggtaaac caaaggattt taaacgattt attattcaag cgacctcttg
     3361 catagaacat aatgcctttt ccgaattaaa taaaataaca tgtccaacct taattatagg
     3421 aggtgctaat gataaaattg ttggaaataa tgcttctttt catttagtag aaaaaattaa
     3481 aaaaagcgaa atatttatct atgagggatt gggacacgct acatatgaag aagcacagga
     3541 ttttaatgaa agagttttag agtttttaaa taagtaaaca cctttgttaa aaatatacat
     3601 atgaagattg gaaatttaat gttaaaaata gaaacatgaa aatatgctta actggtattt
     3661 ttactattca taaccaattt ttgatacatt atctactata aatacaaata tagcttcaat
     3721 gtgattatat attgttgtat tggtaaagca cttatacaaa cagaggaatt ttgtaaattc
     3781 atattatacc cacatttgtt aacttatgaa aatataatca aaatttttat gagcttttta
     3841 tataaaaaat ataatgtttg tatacaaaaa tattaaagac attttagtaa gttttgtata
     3901 taagcaaaca
//
LOCUS       DAMJZE010000254         3869 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJZE010000254 DAMJZE010000000
VERSION     DAMJZE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.258"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..548
                     /locus_tag="PC541_003631"
     CDS             <1..548
                     /locus_tag="PC541_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2841050.1"
                     /translation="SHINILIIADIKNKDICLKLNIKYMFNLININRQLYPAENSKNN
                     DKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVYVLINT
                     LYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIMNKNKG
                     NKEGDSNESNRFDTESYGNNS"
     gene            502..930
                     /gene="ytfJ"
                     /locus_tag="PC541_003632"
     CDS             502..930
                     /gene="ytfJ"
                     /locus_tag="PC541_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2841051.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1178..1618
                     /locus_tag="PC541_003633"
     CDS             1178..1618
                     /locus_tag="PC541_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2841052.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            1634..3085
                     /locus_tag="PC541_003634"
     CDS             1634..3085
                     /locus_tag="PC541_003634"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2841053.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3176..3844)
                     /locus_tag="PC541_003635"
     CDS             complement(3176..3844)
                     /locus_tag="PC541_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2841054.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1622 a          355 c          664 g         1228 t
ORIGIN      
        1 tatctcatat aaacatctta ataatagctg atataaaaaa caaagatatt tgtttaaagc
       61 ttaatataaa atacatgttt aatttaataa atataaatag acaactttat ccagctgaaa
      121 atagtaaaaa taatgataaa aaagagggga tgaagaataa tatagattca agtatattat
      181 tagctgatga ccttttgagc atttatagat tattaaaaaa gataaaaata catgaacttt
      241 attcaaatat taattttgga actggaaata ttggcttaac atcttctgta tatgtattga
      301 taaatacttt gtatggaaat ttatttaata tgattgatgc agaaaaaatg tatttaaatg
      361 taaatccaga tttcacaaaa gattatgttt taggaaatat aaggatacat ataaggccaa
      421 gaataaaagc tttatttaat attataataa tgattaataa aattatgaat aaaaataaag
      481 gaaataagga aggtgatagt aatgagagca acaggttcga tacagagtct tatggaaaca
      541 actcttgaaa caattaaagg ttctatagat gcaaatacta ttataggaga ccctataaaa
      601 acagatacta cagtggttgt acctatatca aaggttacaa ttggctttgg tataggagga
      661 ggagaatact caaaaggata tgatgataaa gatagggaaa ttgaattaaa aaatgaaaaa
      721 tcagatacga attttgctgg aggtagcgca ggggctatat ctgtacaacc agtagcattt
      781 gtagtagttg aaagtggaga gactagaata atgagtttag atagtaatat caatctagtg
      841 gataatatat tatctataac tcctagagta ttggagaaaa ttcaaaatat atcacaaagt
      901 aataagaatc aagataaaaa caatatgtaa gaataaaata tttttaaata tgtatatagt
      961 tttaagtaaa gtagtggggt taatataaat cccactattt tgttgtgtac tttaaacatt
     1021 tatatctaat aagattttag tttaaaacaa tttaaataga taataaagtt gatatatagt
     1081 ataattataa aaagaaaaga agtattatat tatagatata taaacgcatt tgagatataa
     1141 gagggagtta taaatcatta gttttgaggt gaatgttatg agcgataaag aattatctat
     1201 tttgttgtta gaaagtatga ataattttca taatttaata aagattatta ataacgaaag
     1261 atataaaaaa gataaggttc ttacagaaag acagttcttt gccttagtaa aaataagaaa
     1321 acatgataaa atagagctta aaaatctaag tagagatttg catgtatcta cttctagtct
     1381 gtgtatacta cttaataaat tggttgagca agagtatgta tatagggaag aagatagtag
     1441 agatagacga aatacttttt atggaataac taaaaatgga gaaaagatat tggataatga
     1501 aatactaaag tttgtatcta taataagtga taaaatggat tgcctagata taaataataa
     1561 agataaatta tttacttctt tggaagagtc taaaaatata atagaacaat tattttgaaa
     1621 aggagaattt attatgaata taaagtattc taaagaaata ttaaaaagtt tagaaaatgc
     1681 tataacagta gtagctctat ttgagggaga agatgtttct acgacatttg ataaaataaa
     1741 tatgataagc agaaatttta attctaaaga aaatagtata gatactgtaa cattggttga
     1801 aaataacaat ttggtaaatg tttgtcttgt tggttttggt aagagagagt tattgaatag
     1861 agaaaaaatt agaatcatag gtgggaattt gtgtaaaaaa ttaaaaaata tattgaaaaa
     1921 tgaagcatat aagagtagtg atgttaatgt actgaatctt aatttaaata gtgatgaaat
     1981 aggttgtttt gtagaaggga tattacttgg aaattataag tttgataaat ataagacaaa
     2041 aagtaaggat tttgaaagaa aagaagttga tactataact atatttactg aaaaagaagt
     2101 tgagtgtcaa atagaaaagg ctaaaatatt agctaattca actataatag ctagaaatct
     2161 agttaatgaa ccatcaaacg taatatatcc aaaaacatta gcagtagaaa cagttaaact
     2221 tggtgcagag tttgggttta atgttgatgt ttatgaagaa gaaaaaataa aggcattggg
     2281 aatggatgct tttttcgctg tttcaagagg ttcaattaat aaaccaagat tcatagtaat
     2341 gagatacttt ggagataaag aaagtacaga tatattaggt ctagtaggta aaggattgac
     2401 ttatgatact ggtggatatt ctttaaaatc aaatgcttct atgctagata tgaagacaga
     2461 tatggctggt gctgcgagtg ttataggtgc tatgtgtgct atatctcaaa gtaaattaaa
     2521 gaaaaatgtt atagcagtag tggcagcatg tgagaatgct ttatcaggag gttcatacaa
     2581 acctggagat ataattagtt ctatggcaaa gaaaactatt gaagttttaa atacggatgc
     2641 agaaggtaga cttacattag ctgatgcaat atactacatt ataaacaatg aaaaagtaac
     2701 aaaggtggtt gatgtagcta cattaactgg tgctgcttta atattgttag gtaatgtcgc
     2761 aactcctata gtaacaaata atgatgattt ttattgtgaa ttagaaaaag ctgccacttt
     2821 atcaggggaa agggtatgga agatgccaat ttatgatgaa tttaaagata tgataaaagg
     2881 agaagaagca gaccttaaaa acactggtgg taaaaatgct ggatgtataa cagcaggagc
     2941 ttttattggt gaatttgttg ggaatactcc ttggatacat atggatatag cggggacaag
     3001 tacatcatca aaatcaattg gatacaaagc aaagggggct acaggagaac ctgttagaac
     3061 attgtactat ttggcagaaa tctaaaaaac aattataaaa tataatttat aaagccacgt
     3121 ggatattttt gtaatttaag attatctatg tggctttatt ttttatttaa ataaattatt
     3181 tactcttttt ctttttttca agtataaaag tatctctatt aaataaaaga gcaggtatgt
     3241 acataatact agtaacaaaa ataaacgtaa aaatagcagt tgtgctatga cttaaaaaac
     3301 cataatagta cctagagttg gtaaatgtgt ttagtaaaaa tatgaatgaa aaataaataa
     3361 aacatatagc taagttatct ctaagtgcat atctgttaac tctaaatttt ctaacatcaa
     3421 ccatatatat atctgcataa attatcaagc acttagagaa gataaagata aaatatatta
     3481 agctaggaaa ttcatagccc aagtttttag aaaaaatgag ttctccaact gaacaaacta
     3541 aactccaaga aaaaaatata ttaaaaagat gatactgctt aaaaattagt atggctatac
     3601 acagatatat acaaatataa ttgattccaa ttggtaaaga gtttagagta ctataattac
     3661 ctgaagaaat aagtaataat tgctctagag ccataattat tacaagtaat gtacaaatta
     3721 tcttctcaac tgtatagcta taaggtaaaa tatttttggt tagttttgga caatagtata
     3781 aaaataatcc aaatataaag agacttacaa attgttcact agaaaaaaga aaaattttaa
     3841 acatataaca ttctctccta taataaatt
//
LOCUS       DAMJZE010000255         3788 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJZE010000255 DAMJZE010000000
VERSION     DAMJZE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.259"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            78..1130
                     /gene="ytvI"
                     /locus_tag="PC541_003636"
     CDS             78..1130
                     /gene="ytvI"
                     /locus_tag="PC541_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2841055.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1220..2446
                     /gene="pepT"
                     /locus_tag="PC541_003637"
     CDS             1220..2446
                     /gene="pepT"
                     /locus_tag="PC541_003637"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2841056.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2653..3705
                     /locus_tag="PC541_003638"
     CDS             2653..3705
                     /locus_tag="PC541_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2841057.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1469 a          396 c          701 g         1222 t
ORIGIN      
        1 tttattttgt attcaaataa aactagtatg aaatggacaa aataaaaata taaattagaa
       61 gaaaggagat ttttaagatg agcatactag aaaataattt tttatacaaa ttaaaaaaca
      121 acactatctt tttttgtata tatacattgt tatttatttt tatttacaaa gcgtttccat
      181 acatagctcc attttttcta ggtgctataa tagcacttat gattaatcct atatcacaaa
      241 agttagaaaa taagtttcgt ataaataaag gtatctctac tcttgttcta agttttcttg
      301 cagtggctat agtcagtaca gttactacaa taatagtaat aaattcaatg aaagaattaa
      361 tgggattttt aaataatata tctgctaatc ctgaggatat aagtaatact attatgtatt
      421 ttttaaataa aataaatgac tttatgaaat catttcaaga aatagccaat tttgatgtag
      481 aacaattagt aaacaaattc agtggtgaag taatgcaaat tacaaaaaat cttcttacta
      541 gtatacttgg attggctact tctattccat atattataat atttataatt accttgttta
      601 ttgctacgta ctttatagca aaagaccttg ataagattga aaatagtttt tataatatgt
      661 ttactgttga tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg ctttcacttg
      721 ttggatatat aagggcctat acaatactta tggctataac attttttgca atctggggaa
      781 gttttgcttt atttggattg aaatacggtc taatagtagg gtttgtagga gctttaatgg
      841 atttaattcc attcctaggt ataataacaa tatatcttcc agtaattgtt tattatttcc
      901 ttataaaaaa ttattttata gctataagta tgactgtgat attttttgtt ttgtctttga
      961 ttagagaaat acttgaacct aaactagtat cagtaaatgt tggactaaat ccattagcta
     1021 ccctagcagc gatttttatt ggaatccaag taaaaggaat aattggagta attttttgtt
     1081 tgggtttggt atgtatgcat gatatactta agaaggtgga tattttttaa ttataataat
     1141 tgatagattt tttaaaaata aaattttatt cacaggtaaa taaaaaaata ttaatcttat
     1201 taagaaagag ggtatatacg tgaaacaaaa agtagtggaa agatttttaa aatatgtatc
     1261 atttgataca acatctaatt ctcagtgtga gaactgtcca tcttcagaag gtcaaagagt
     1321 tttagctaag tatatagttg aagagctaaa aacaatgggt gttgatgatg tgagtttaga
     1381 tgaaaatagt tatataatgg ctactttaaa aggaaatact gatggtgtgg atactatagg
     1441 attcatatca catttagata caattgaaga tgttagtgga aaagatatta aaccaagaat
     1501 aatagaaaat tatgatggaa aagacatagt tttaaatgaa gcgttaaatg ttattacgta
     1561 tgtaaaggat tctccagagt tggaagaatt taaaggtgac gatttaatag tcactgatgg
     1621 aacaactcta cttgggtctg atgataaagc tggtatagct gagatagtga ctgcaataga
     1681 gtatctaata aatcatccag aaattaagca tggagatata aaaataggat ttacaccaga
     1741 tgaagaaatt ggtagaggtg ctgatttatt tgatgttgaa aaatttggag caaaatatgc
     1801 atatacttta gatggaggaa tagttggaga acttgaatgt gaaaatttta atgctgcaaa
     1861 tgcaaccatt actatacatg gtagaaatgt tcatccaggt tcagctaaaa ataaaatggt
     1921 aaatgctata catatagctg ctgaaatatc agaaatgttc ccagcagatg aaagaccaga
     1981 aacaacagaa ggatatgaag gattttggca tttaaattct ataggtggaa atgtcgagaa
     2041 tgtaagtatg gcttatatta ttagagacca ttgtaaagaa aaatttgaaa atagaaaatc
     2101 tattatgata gagaatatag aaaaaataaa taagaaatac gataatagag tggaattgga
     2161 tttaaaagac tcttattaca atatgaaaga aaagattgag ccagttatgt ttatagttga
     2221 tatagctaaa gaagctatgg aggaattagg tataaaacca cgtttagttc ctgtaagagg
     2281 tggtactgat ggagcaagat tatcttttaa tggattacct tgtccaaata tatttactgg
     2341 tggattaaac ttccatggga aaaatgagtg tataccagtt agttctatgg agaaagctac
     2401 taaactaatt gtaagaatag ctgaaaaata tgctgaaaga gtttagttaa aaagtattta
     2461 aaaattaagc atttaaagtt ataatagcta tatcattctt tataagtcaa gctgattata
     2521 agcttgtgtt gtacttaaaa tggtatagct atttttgtaa ttatttttat aaaagataat
     2581 tatttttagt ttagggtatc aataaaaagt atgaatatca ataggtatta acatttaaaa
     2641 gggggaattg tattgaaaat taaatctaaa aaaataatag catttgcact ttcacttggg
     2701 attatgttgc caagttgtgt atatgcagac actattagta aaaatcaaaa tgcacaggta
     2761 gatatgaaca attatcttca aggtagaaaa tctccaaagg aggaaaaact tattggtgaa
     2821 gatagatttg atacagcgat aaaaatatct caaagtggtt ggaataatgg ttcagaaagg
     2881 gtgtttctag ttaactcaaa ttcattgcca gatgcattgg cttcaactcc tttggcaagt
     2941 aaattggatg caccaatact gcttacaaat aaaaatagta taccatataa tgtaacagag
     3001 gaaataaaaa ggcttaatcc agcagaagtt attttaattg gaagtgaagg agctatttca
     3061 tcaagtgtaa aagaatctct tgaagatatg gaagtattag tcagtagaat cggtggagca
     3121 gatagagaag aaacatcttt acttcttact agacagctag atgatactgg tgatttaggt
     3181 gtctcaaaag tggcagtggt taatgggtat aatggactgg cagatgcaac ttcaatatca
     3241 agcccagctg caagtgataa tacagctata atatatacag gtaaggatag cataagaagt
     3301 gaggcaaaga attttattac acaaaactca actaagacat acatagtagg tggggagtac
     3361 agtataagta aaaaacttga aggacagtta gtaaactcag aaagactagc agggacagat
     3421 agaaaagata ctaatgcgaa agttttagaa aagttttatg gaaaatcaag taaggttaat
     3481 aatatgtatt ttgcaaaaga tggaagtggt agagaagctg atttagttga tgggcttgct
     3541 gctggagtat tagcatcaaa aacaaagtca cctgtagtac ttgcaagtgg aagtttaagt
     3601 agtgcacaga aaagttttat aaaaaaagta aaggctgata aatttgtaca agttggtgga
     3661 ggtaaaaata gtaaaccata tgcagaagca ttagccttac agtaatattg tgtaaatcaa
     3721 aattaatata taataatagc aagtaaacca agttgcactt caatatatat aataaaataa
     3781 gtaaacta
//
LOCUS       DAMJZE010000256         3776 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJZE010000256 DAMJZE010000000
VERSION     DAMJZE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.260"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            83..1195
                     /locus_tag="PC541_003639"
     CDS             83..1195
                     /locus_tag="PC541_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2841058.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1403..1879)
                     /locus_tag="PC541_003640"
     CDS             complement(1403..1879)
                     /locus_tag="PC541_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841059.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2078..3703)
                     /locus_tag="PC541_003641"
     CDS             complement(2078..3703)
                     /locus_tag="PC541_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2841060.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1311 a          609 c          419 g         1437 t
ORIGIN      
        1 tgtatgcaac tctatttcaa aattattttg tgatatatat atgttataat aaatgtataa
       61 aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt
      121 tgtttacctg gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac
      181 ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa
      241 tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa
      301 taaatatagt gaaaatatat ctttagctac atataataat tatgttagta tttgtaagaa
      361 ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca
      421 aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa
      481 tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat
      541 ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca
      601 aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttggc
      661 tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt
      721 tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt ctgttattct
      781 caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc
      841 tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa
      901 taataataat ttgttatttt tcaataagaa atgtgaacct attgctgaag atgttatgag
      961 taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt
     1021 aagacactca catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga
     1081 gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga
     1141 aatggataaa gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta
     1201 atttatctta gctttaaatt agcttagctt attgagatag attcattaat attaaatgta
     1261 tctatctcaa attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt
     1321 attatacttt tttacgaaaa attatacttt acaataaaaa agatattccc ttatagaaaa
     1381 tatcttttta aatactatat tattatatac ttgctaacat tggaatatct aacatcttct
     1441 tcattacatc atctactttt tttgctttat attcattgta tccaattaaa tatccttttt
     1501 catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac
     1561 ttaaattata aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa
     1621 aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt
     1681 ttgtactagc ataaggacaa aaacctttat tgtgttgatg tgctgaatgt cctccacaat
     1741 aataacgtgt taactctgat ttatttttat tatgatgacc tcctgatgaa ttatgccctc
     1801 ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactaatt actacagcta
     1861 ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt tattatatca
     1921 tctactatat tatatttaaa ttcttaatat acaaaatatt actctattct gctaataaac
     1981 atgtttaaac aatataaaaa acctcctaat atttgattaa aaatttacac actcatgcat
     2041 agcatgtctc aaatattaaa aggttttata ctaataatta tttatctttt ccatacattt
     2101 tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc atttttctat
     2161 ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat ataaattcat
     2221 ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta tttacagaag
     2281 ttatactttc taggtctaag tggttagtag ggtcatctaa tacaagtaca tttgcatttg
     2341 aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata acttgagctt
     2401 cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt ataaaacttt
     2461 cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct actccgtcaa
     2521 aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc catttatact
     2581 ctccactgtc tggttcaagt tctcccataa ctatgtttaa aagtgcagta gtagctattt
     2641 catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca ttatcaagaa
     2701 ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca ttcccaattt
     2761 ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt atatcttcaa
     2821 cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt gaagcattag
     2881 aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc tttttatttt
     2941 ggtccttagc caattgtaaa gccaattgac ttgactcata ccagaaatca tagtttccaa
     3001 cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt tgatttagga
     3061 aatgtctatc atgtgatact attataacta tagattcttc taaatccatt ataaaattat
     3121 ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt aatagtattt
     3181 caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca ccaactagtt
     3241 ccttcatttg tttgtaatgc atatctttcg ttatgccaag acccataagt attttctcag
     3301 cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt tcggcagctt
     3361 ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt tctttcatta
     3421 tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct tcttcatatt
     3481 caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat atactacctg
     3541 tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc gcaccattag
     3601 ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct ttaaatagtt
     3661 ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata ttttcctttc
     3721 tttttgctca taatttattt tatcataaaa ctttgataac atttttatta tatatt
//
LOCUS       DAMJZE010000257         3644 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJZE010000257 DAMJZE010000000
VERSION     DAMJZE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.261"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            202..1578
                     /locus_tag="PC541_003642"
     CDS             202..1578
                     /locus_tag="PC541_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2841061.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1889..2560
                     /locus_tag="PC541_003643"
     CDS             1889..2560
                     /locus_tag="PC541_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2841062.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2749..3546
                     /locus_tag="PC541_003644"
     CDS             2749..3546
                     /locus_tag="PC541_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2841063.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1379 a          405 c          676 g         1184 t
ORIGIN      
        1 tagatgtttt tggtgcaatt taaattaaaa gatatatata aaaatttaaa atattccaaa
       61 cagaaattat aaaaattact atcattgtct ttatacaata tttatactat aatagaataa
      121 taatttagag gaaagctcta aaaataaaca aattaagggt cttaacagta gtgattttat
      181 gttaaggagg tataaagaaa gttgaaaaca atagttagaa gacttattct ctacgcagat
      241 ggaatgcgtc ctactcaaat aatggtatct gggtttgctg cgattattgt aatcggagca
      301 ttgttgttaa cactcccgat agcgtcgcag agtggagaga gtataggctt attaaatgct
      361 ctatttacgg ctacttcagc ggtttgtgtt actggtctag ttatggtaga tacagcaact
      421 tattggagtt tatttggaca gatagttata ataacattga tacaaattgg tggtcttgga
      481 tttatgaccg tagccacaat gttttcactt atggcacgaa aaaaaataca actaagagaa
      541 agattattaa tacaagagtc attaaatcaa gctgatttat cgggacttgt aagacttaca
      601 agatttgtat taataataac cataacaata gagggaatag gtgcactggt tttatcaact
      661 gtctttattc cacaatttgg tttgtctaaa gggatatggt atagtgtatt tcacgcaata
      721 tcagcgtttt gtaatgccgg atttgacttg atgggcagtg taagtggccc atttacatcg
      781 ttaaattctt atgtaaacaa ctttacagta tcaatgactg tttgcgcact tatagtgctt
      841 ggagggctag gattccctgt tgtacttgat attgtaagaa aaagaagatt ttcaaaatta
      901 aatgtgcatt ctaaggttgt attgttttca actgctacac ttatttttgt gggtgcatta
      961 tttatttttc ttatagaatt taatcaaaaa gctactatgg cggacttgcc tttaaaggga
     1021 aaagtactat cagcaatatt tcaatcagta acagcaagaa ctgctggttt taatacgctt
     1081 gatttagcta ctttacgaga aagtagtgta tttgtaatga taattttgat gtttattgga
     1141 gcatcacctg catcaactgg tgggggtata aaaacaacaa cactagctgt tttaatcatt
     1201 acagttagaa gttttctatc aggaaaatca gatatagaag cttttgaaag aagattagct
     1261 ccatcaacta taaaaaaatc tcttggtata tttgtaatca gtatatcagc agttattttt
     1321 ggaacattga ttatttcaat aactcagcct aattttacat tagttcaatc agcttttgag
     1381 gttacatcag cacttgctac agttggttca agtttagctg gaactccaaa cttaaatgct
     1441 ttaggtaaga ttataataat aatctttatg tttatgggta gagttggttc tcttacttta
     1501 tttatggcta tactttctgg tggtagaaga aagagtcaac caatcagata tgcagaaggt
     1561 aaaattatgg ttggttaagt taccaaggag tagtataaat ttataatggg tttagagtag
     1621 cactgtaaat ctgttgataa aataaatgag cctagatgta attatatgaa gaaataaaat
     1681 caactaatga acaaaattaa ttaatgaaat aaaaaaacag ggaaataaaa tacaaataaa
     1741 aaggtaaatc aaaattaaat taatctgtgt gtataaaatt atatattgtg tataagtttt
     1801 gtatgtgtaa agtatataag ttttgtatgt gtaaacaatt aatttaagag atattatata
     1861 aattgtgtat tagggaggaa gtttcgagat gaagcaatat atagtcattg ggtgtgggag
     1921 atttggaagt tcagttgcgt ctactatgca tcttttagga catcaagtaa tggcaataga
     1981 caaaaatgaa gattcagttc aaagtatatc tgacaaggta acccattcac ttatagtgga
     2041 tgttactgat gagcaagcgt taaggtcatt aggtttaggt aactttgatg tagcagtagt
     2101 tgcaataggt tctgatataa gggcatctat aatggcgact cttatagcca aagaaatggg
     2161 tgtagagttg ataatatgta aggcaaagga tgaattacaa gctaaagttc tttataaaat
     2221 tggtgcagat agagttgtat ttccagaaag agatatggga gtaagagttg cacacaattt
     2281 agtttcggat aatatattag accatattga acttgaccca gagtattcaa ttgttgaaat
     2341 cgtaactcca aatagttggg ttggcaagac acttatagag cttgaattaa gagctagata
     2401 tgagataact gtacttgcta taaaaacagg taaaaatata aatgttacac cttctccaga
     2461 tgaggaactt acagccggaa gtatcctagt tataatcggt caaaatacta gtataacagc
     2521 gataacatct ggaaataagg ggataattag aagaagataa tttactattt aatatatatt
     2581 taattgcaat gaaagcagat agtatcatat aattatgaat agttatatga tactattttt
     2641 ttaatcgaag gtagtattta tttgtaagat tagataaaga gaagttaaat taaaagtaag
     2701 gaggctgtgc taatataaaa atttataagt tattagcatt tagataaaat gattacaaat
     2761 ataaacagta aggataatga aaagttaaag tatacaagag cactattaaa atcaaagaat
     2821 aggaataaag agtcaaagtt cataatagaa ggatacagaa tagtaatgct tgcacttgaa
     2881 tgtatggcaa accttgatta tgtatttatc aatgaagaat ttgaaaataa gaaagaacat
     2941 gtaaaactat tagaagattt ggataaaaaa aacacaaaga tatacaagac tactaataaa
     3001 aactttaaag aattagtgga tacagaaaat actcaaggaa taataggtgt agtttcattt
     3061 aagaaaaaaa aattaagtga aagtataaat aaaaaagata aatttgtatt gattttagac
     3121 agaatacaag acccaggaaa tatggggact ataataagga ctgctgattc tgctggagta
     3181 gatgccataa tagcactaaa gggatgtgtc gatatataca acccaaaagt aattaggtct
     3241 actatgggtt ctatttttga tatgaatata attgatgctt cacaagatga aactgtggac
     3301 atgcttaaat cattggattt taatatagtt tcaagttact taaatacaga aaatttttat
     3361 gacaaaatag attatggctc aaaagtagca ttggtgatag gaaacgaagc aaatggaata
     3421 aatgaagaac ttgtatcaaa gtctgatatt ttggttaaga tacctatata tggtaaagcc
     3481 gagtcgttaa atgctgcgat aagctctgct atactgatgt atgaaataaa aaaatactta
     3541 atttaatgta ttgtaataaa tataatgtca tgttataatc ttgaattaaa tagtagagta
     3601 tcaaaaatag tcaatatata ttaaaaaaat aattaaatta atta
//
LOCUS       DAMJZE010000258         3634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJZE010000258 DAMJZE010000000
VERSION     DAMJZE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.262"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            317..2128
                     /locus_tag="PC541_003645"
     CDS             317..2128
                     /locus_tag="PC541_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2841064.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2216..2797)
                     /locus_tag="PC541_003646"
     CDS             complement(2216..2797)
                     /locus_tag="PC541_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841065.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNY
                     TNYYMEFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(3233..3439)
                     /locus_tag="PC541_003647"
     CDS             complement(3233..3439)
                     /locus_tag="PC541_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841066.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT         1371 a          369 c          499 g         1395 t
ORIGIN      
        1 caaatttaaa aagagacata aagacattat acaaatatat tattggggtg atattttgta
       61 agaaaagaag tgatgcagta aattaattga gtaatttaaa aatgatttaa tgataatgtt
      121 gatgaatgtt taaaaagaaa ttttagaaaa acgtatattg ctgtttgtgt tggaggtgat
      181 gaatttgtag aatttatagg gtataaatga ttaaactatt taagataaat acatatcact
      241 gtaaaatatt gataatcatt ttttataaga gattgattat ttgcaaatta tatatattac
      301 aaatgaggta acaataatga agaaaaaata tgatttttta atttattctg ctatttggct
      361 agttattatt attcttttta tattttctct atatcatagt atagagcata ttaaaattat
      421 aagtcacact ggtactattc gcagtgaaac tcaaaaggtt gttaagcaag aattgaataa
      481 tgaaagaaat gatgacttga taaaacgact ggataatatt ctgataaagc taagaacagg
      541 taatggagaa aatggatttc aaagatgtga taataaagaa tttcaacaaa aactaaatca
      601 aatggattct atgtgggaat ctatgaagaa agaaattata aaagttagaa atggtgcttc
      661 aggtgataaa ttatataaat tgagtgaaga gtattctgtg ttgtctaatc aaattgtttt
      721 tatatctgaa aaacactcta atgcaaaatt gtattctttt gctactgcac tatttattta
      781 tcttatattc tcaacaataa gtcttttaat ttgggaatat tataataaaa aaaggtttaa
      841 aagaatattt tatactgata atttaacaaa aataaaaaat caggttgcat ttgaaaacag
      901 agcgattgaa attttgcaca atgcatctaa caaagagtat gtactgctaa atattgatat
      961 tgacaatttt aaatatatta atgatacaca tggatatgaa tatggagata aagttctaat
     1021 aatagttgca actgttcttt ctaaaacttt taatataaaa gaaacttgtg caagaatttg
     1081 ttcagataat tttgtaatac ttgcaaaata tagagattct cttttagagg atattcggga
     1141 aatgctaact aatgctataa tctctgaatt agatatgaat gtaactcaaa ctatatctta
     1201 ttgtatagga gcttacttag ttgaaattga taatttaggt tataaatcta tcaattctat
     1261 gatggataag gcaaatattg cacacaaggt aagtaaaaca agaggaattt cctccacagt
     1321 atggtataat gaaaatctgt taaaacagtt acaaatggaa aatagtattt ataattatat
     1381 gtataaagcg atagaaaatg aagaatttca tatgtatttg caacctaaat ttcaaatatc
     1441 ttctttaaat gttgtgagcg cagaagctct tgtaagatgg ttttctccag aacttggttt
     1501 tttatcacca gatgaattta ttccattgtt tgaaaaaagt ggatttatta ttgaattgga
     1561 cttttatatg ttaaaaaaag cgtgttcatt tgtaaaaaaa acgtttatga aaaaaaatca
     1621 atatacatat ccgattgcag ttaatttttc aagggttact atttaccaaa atagttttta
     1681 tcaaaggttt ttagatactg ttaaagaata tgaaattcct tttaagtata tagagataga
     1741 ggtaactgaa agtgctttta atgaaatatc tcaaccagtt atttcaattt tagaggaact
     1801 taaaaagtta ggatttttaa tttctatgga tgattttgga tcaggatact cttcattaag
     1861 tcttttatgt tctctatcta ttaatggatt gaagttagat aaaagcttac ttaaagaaac
     1921 atttaaccgg gagaaagtat atagtattat tcagtgtatt attgaaatgt cacatcgtat
     1981 aggcatgtct gttgtatgtg aaggtattga aactaaaaaa gacttggaat ttttaaatac
     2041 agttaaatgt gatgtaggac aagggtttta tttttctaaa ccgatagaag aaaaagaatt
     2101 ttttaacaaa tatgtaacta aaaaataata taattatttt tatcagtcta tacattgagt
     2161 cattttatat ctcgttaaat gactcaatgc actgttatgt attttaaaat ataaattatt
     2221 taaaacgttt tttatactta tatagaaatt cttctatatt ttttgattgt tctcttattt
     2281 caactgcttc tatatctagt aaataaagta tagaatcatt tgtaagttca acatattcac
     2341 catcttctga accgagtgca tgtctctcaa tttttaatcc tttttcttca taatattttt
     2401 ttgtttctat atataatgat tctcgctttt gtatgtagtc tttcacttca ttatttggaa
     2461 agttatatga atccactaat gtttcgccat ttataaaagt tttctcggta gcaacttttt
     2521 catcccaaca atggatttct tcccaagtaa aaaatatctc agttccatct ggaatgaatg
     2581 aaaacctggt aataatatat aaataattat tgaaatttac ttcataatat tcaggaatag
     2641 cttcaaattc catatagtaa tttgtataat taaagttact actatctttt tttacaaatg
     2701 gtattttctc aataaattta tatattgatt gttcattttt aaaaattcct atatcatagc
     2761 ttctttcaaa ttgattaatt tttaataaat atgacatata ttctccttat ttgttgaaat
     2821 ataattttta taactctact aattttaaac atttttaaat aaatatttgt atatacctta
     2881 tataatatta tagtaattag ttttaaatca atagaatagt atgtatttat ctaatgcggc
     2941 aatataattt aaagctggtg ttgtagaaaa actaagagtg aatatagcaa agagaagcaa
     3001 tagtatttta aactttgtct ctcttatttt ttgaaaacta acattaaaac ataaggtata
     3061 aataatatta tcatataaat gaaaacaaat ttgaattttt ctctggtcga tgtatatagt
     3121 aagtttgttg atatgagaaa aattattatt gataatatac atgcatagaa aggaaatggt
     3181 atataatatt ttcttaagtt tcttttttag gcatttctat tggcatttta ggctatagta
     3241 ctaaatacat taaccaacaa aataatgaaa taaatatata tatagaaaac ttaattttcg
     3301 tgggaagaaa aaaagcaata atagcaagta ttattagaga ataacgaata atacgagtaa
     3361 catttctttc tttttctatg atagttaaat cccagtgatt ttttataaat tcaaggtctt
     3421 tttcttgact ctgattcatt taatcatctc ctgttaatag tataatttta aagctattac
     3481 tttgttttat tttaacatat ttatacacta aagtgaattt tatgattaga tttatttaat
     3541 tatttctgaa ttaaaactta ttagaattct ctattttgat aatttcaaac tctattgatt
     3601 gtatgataat tgaaactata ggatatgtag gata
//
LOCUS       DAMJZE010000259         3633 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJZE010000259 DAMJZE010000000
VERSION     DAMJZE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.263"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            147..1727
                     /locus_tag="PC541_003648"
     CDS             147..1727
                     /locus_tag="PC541_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2841067.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1901..2347
                     /locus_tag="PC541_003649"
     CDS             1901..2347
                     /locus_tag="PC541_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2841068.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2368..3411
                     /locus_tag="PC541_003650"
     CDS             2368..3411
                     /locus_tag="PC541_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2841069.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1461 a          336 c          691 g         1145 t
ORIGIN      
        1 aattcaaaat tttttgttat tttaaattat ttcaaaactc ttagttttag tgattttatt
       61 tatgatattt atgggattaa aaagatttca atagatattt ttggtaagta tttaagaagt
      121 ctttaacttt ataggagtga taatttatgc taataggtac atctaaatta agcagaaatg
      181 ttgatactgc atgtgtagaa gaagtaggga ttccaatgat tgtattgatg gaaaatgcgg
      241 ttatatctgc aatgaaaaat atggatatag acatgtataa tagttataca gttgtatgtg
      301 gtgttggaaa taatggagga gatggtcttg gaatagcaag acatctaaat acatatggaa
      361 aagaagtaaa cgtattttta gtaggtaatc ttgaaaaact aagtgaatgc tctaaaataa
      421 attacaatgt acttttaaat atgggaatta atattataaa tattaataaa aatgatttaa
      481 gtgaaaaaaa ttatggagag gaaaatgaag tagataaaat tattaaagtt gagaaacaaa
      541 atattataaa aaataaaaat ttaacaattt ttaaagatag agtatctaaa agtgatgttg
      601 tagtagatgc tatatttggg actggattaa aacgtgatat aactggtatt tttaaagaag
      661 ttattgacat agtaaacgat aatagcaaaa atacgtattc tatagatatt ccttcaggta
      721 tagatagcga taatggaaat gtattaggaa cttgtattag agcaaacaaa acgattagct
      781 ttgagtttta taaaagaggt tttctaaact atgacactag cgcacttata ggtgatgtag
      841 ttgtagaaaa gataggtgtt ccagatttta ttacaagaaa ataccatgat gaagagttca
      901 taactgataa gacttttgta aaaaataata ttagaagaag agataagcat ggttttaaaa
      961 gtgattttgg aaaagtatct atagttgcag gttcaaaagg attttatggt gcaagtttca
     1021 tagcaactga atctgctgtt aagagtggga gtggacttgt aacattaatt agtagtgaag
     1081 atgtgcaaga aaaggtatct attagactta cagaagcaat gactttaaat tttgaagaag
     1141 aaagattaaa taaattactt aattctagtg atgcaattgg ttttggtcca ggtatgggag
     1201 ataatagtca aacatttgat aaattactta agatagtaga aaattctaat tgcccaatag
     1261 tattagatgc agatggattg aacgttatga aggacagatg ttataagttt ttagaatgga
     1321 aaaatagatt tgtaattaca cctcatttgg gagaaatggc caggttgaca ggagattcaa
     1381 ttgggtatat aagagaacat agagtagatg ttgcaaaaga atttgctcaa aaatataatc
     1441 tggtggtgct tttaaaaggt tatcaaactg tcataacaga tggtaaaaaa acatatataa
     1501 atcctacagg aaatagctgt atggcaactg gtggaatggg tgattgtttg cttggaatga
     1561 ttacttcatt tataggtcaa ggtatggata tattggaagc tacagttagt ggagcatata
     1621 tacatggcta tataggagat aaattgagca aagcaatgta tacggtaaat gctacggatt
     1681 taataagtaa catttcatta acaatgaaag agttgttgga tatataattg gtgatatttg
     1741 atactacttt gtaacaattt taacaaagta tgttgatata atactattaa ttaattaatt
     1801 tattttattg ttttgtaatt taaatttata ttcaaaaaca ttgtataaac atataattta
     1861 tatagggttt tataattcat atgaaaagag ggtagatagt atgaagctaa tatggttgat
     1921 agtagcagtc ttatttggaa tagcagaaat gttaacacca agcttaacat tgatatggtt
     1981 tagtgtagga gctgtaatac ttatattttt aagtagcttt atagagagta ttttcttaca
     2041 aattttaata tttgcagtaa tttctatagc aatgttagtc gtagctacta aaaaaatagt
     2101 aaaaaaagat aaaggctata aatctaacac taatttacaa gcaatgatgt ctaaaaaagg
     2161 tattgttaca gaagagattt ctccaaataa cacaggtcta gttgtagttg aacatgaaac
     2221 atggacggct atttctatag atggtgaaaa gatagaaaaa ggttctacag tagaagtatt
     2281 aaaaattgaa ggggtaaaat tagttgttaa aaaagtagac gccacagtta atgtgacaaa
     2341 ccaatagaat aaaggaggat ttatagtatg ggtactaaaa ttgttttaag tattgtatta
     2401 atagtagtag tcgtagcaat aagtttaact tgtataagag ttataaaaca atctaaagta
     2461 ggtataataa tgagacttgg taagtttcaa aaagtggctg aaacaggtgt acatttctta
     2521 ataccatttt tagataaaat ggcatatgta attgacctta gagaaattgt aatagatttt
     2581 ccacctcaac cagttataac taaagataat gtaactatgc agattgatac agttgtatat
     2641 tataaagtta cagacccagt tagatatgtg tttgagatag ctaatccaat agctgctatt
     2701 gaaaatttaa cagctacaac actaagaaat ataattggtg aacttgattt agatgaaaca
     2761 ttaacatcaa gagatataat aaatgtaaaa atgagaacta ttcttgatga agcaacagat
     2821 aaatggggta taaaagtaaa tagagtagag ttaaaaaata taatgcctcc tcaggatatt
     2881 caagttgcaa tggaaaaaca aatgagagca gaaagagaaa gaagagaggc aatacttcaa
     2941 gcagaaggta ataagtcagc tgcaatactt caagctgaag gtgaaaaaca atctgctata
     3001 ttaacagcag aagccaaaaa agaagctatg gtacgtgtag cagaaggtga aaaagaatct
     3061 gctatattag tggcagaagg tgaagctgaa gctataagac agacagctat tgctaaggca
     3121 caaggtgaag ctgaaatgat aaaaagaact caaatggcaa cagcagaagg tttaaaatta
     3181 gttttttcag caatgaaaga ggctgatata gacaataata ttctagcatt aaaatctatg
     3241 gaagctcttg aaaaaatggc tgaaggtaag tcaacaaaac tggttttacc ttcagaagca
     3301 gttaatttcc taggaacatt caaaggaata aaggaagtta tgagtgatga taacaaagaa
     3361 gtacttgata taaaagaagt tttaaatgat aatgaatcat taaaaaaata aaagagtttt
     3421 gttatcatag gaaggatatg gaaattaaag gtcgaattaa ttaaatatag cacaaaaatg
     3481 cacgttgaaa agaagaggac ttataaattt ttaagtcttc ttctttgttt ttattagaaa
     3541 gatgaaatta taaaaataaa ttaacagaat ttttatacaa tttatccttt actaatgtaa
     3601 aaagtgttat ataatattat ataaaacata tag
//
LOCUS       DAMJZE010000260         3586 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJZE010000260 DAMJZE010000000
VERSION     DAMJZE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.264"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(70..984)
                     /locus_tag="PC541_003651"
     CDS             complement(70..984)
                     /locus_tag="PC541_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HDF2841070.1"
                     /translation="MELVKNIHKNKSKDEIFKELLENLKGTIASWDYYVNWNKALENT
                     RKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVIPIVIASRDNEFKILDPSDEDM
                     FKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQKNIKNLVDYVFGVEVGLDTNA
                     RKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKISEKWSIEVPTDKSKRKYDFAI
                     NNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKDGGFTFCWITDGLGWLESSIPL
                     RDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(1003..1917)
                     /locus_tag="PC541_003652"
                     /pseudo
     CDS             complement(1003..1917)
                     /locus_tag="PC541_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(2345..2694)
                     /gene="ssrA"
                     /locus_tag="PC541_003653"
     tmRNA           complement(2345..2694)
                     /gene="ssrA"
                     /locus_tag="PC541_003653"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2852..3307)
                     /gene="smpB"
                     /locus_tag="PC541_003654"
     CDS             complement(2852..3307)
                     /gene="smpB"
                     /locus_tag="PC541_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2841071.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         1180 a          644 c          341 g         1421 t
ORIGIN      
        1 ttttatataa tatataagtc taaattaact tgcaatataa aagcgcctct taatataggc
       61 gctataatgt tataaatttt taattatata ttccaatgaa cctttatcaa ccatatctaa
      121 attaaatata taatccatct tatcaaatgc atctcttaat ggaatacttg attcaagcca
      181 tcctaaccca tctgtaatcc aacaaaatgt aaaacctcca tcttttatat acttatctaa
      241 cttagtaaac tcatttgcaa cagattttaa cttagaacct ccaccattat aaaaattaac
      301 ttctactaaa acaatttttc cattattatt tatagcaaag tcatactttc ttttactctt
      361 atctgttgga acttctatag accatttctc acttattttt ttcactgtag cttgtgttaa
      421 ataactccaa ttattctttt cacacatatt ctttacatat ctttctacaa gagtttccat
      481 actacttcca gttctatttt ttctagcatt cgtatcaagc ccaacctcta ctccaaacac
      541 ataatctact aaatttttta tatttttttg ttttaataaa tctaaaaact ttattttttt
      601 aagaaattct gtagctaatt cgctttcttc atcacttata ctctttctct tcttaaaatt
      661 aaacagttta tatttaaaca tatcctcatc tgatggatct agtattttga attcattatc
      721 tctactcgct attactattg gtatcacact aagaacttct ggatactctt ttaataaatt
      781 tttagtttcg ttttctatat cttcttttcc tataagatag tttaagatat ttaactgaac
      841 ctctatcttt ctagtatttt ccaaagcttt attccagtta acataataat cccagctagc
      901 tatagtacct tttaaatttt caagtaattc cttaaatatt tcatccttac tcttattttt
      961 atgaatattc ttaaccaatt ccatttgatt ccccacctct tcataattag taattagcaa
     1021 ttcagttata aaacctcttc cattcccctt agaatttatg aatcttttag catgtactct
     1081 ttttatatta aaatctgaat acaaatcatc aaagaaagta tcttcttcat ctaaaacttt
     1141 tggatcagaa ttacttaaca tcaattttgt atctatttta tctagaactt tataaaattt
     1201 acttaactca atttgactat catcattaaa attacctttg ttataggaat taaatcctcc
     1261 tatagttact ggtctatatg gtggatctag atacacaaaa caatttttat ctattatatt
     1321 ttctagattt aaataattct tatttaatat ttttactttt agctctcctc gagaatctct
     1381 tttatttaaa agcatggata atttttcaag ttcattttta tcaaaaaaac taggattttt
     1441 atatcttcca aaaggtacat taaattcacc tcgagaattt tctctatata atccattaaa
     1501 acatgtttta tttaaaaata taaaatatga agcttgcttt atacaatctt cattttctat
     1561 atttttttca tttgtaagta tattaaatct atctctaata tcatagtaca tcttttctct
     1621 atctgaagaa ccttcttcgt aagatatgta ttgttctttt aaattagtta gttctgttat
     1681 taactcttcc aaatcatctc tgactttaat atataaatta attaacttac tatttatatc
     1741 attaattaca tacttgttga aaatgtcctc ttctaacaaa tatagaaata gtgcacctgc
     1801 acctacaaaa ggttctatat aagtcatgga actaaaatca caacctgtag gcatttgttt
     1861 tcttagaata tcaagtagct gtgttttacc tccagcccat tttaaaattg gtttcatgca
     1921 acacctctct cagttatatt gttatataat tatataaaat aagattctaa aatacaagta
     1981 ctcatcagaa cactatattt atatcaaatc tttccaaaat ctaagtatta ttttatccta
     2041 acacttttca aaacctctaa ataatataca atattctcta atatttactt actaaaaatt
     2101 taaatctatc tttaagtata acctaaaaca gattataagt tacgtagttt ttaatataaa
     2161 atatacttct acaagtaaag ctttaaatat caattactta tttcataatt aatgactatg
     2221 taccctcaaa aaaacctcat tttctatggt tggggtacgg ttggtggatt tcatcaccaa
     2281 aataaaaaat actagtcaat aaagactagt atttccaagt atttcatcat tcttttttca
     2341 aaattggtgg agacgagggg agtcgaaccc ctgtccgcaa atcttttcct tgctgcatct
     2401 acatgcttag ccaacttcac taatatctca cctcacatag cgccaagtgg caggctctat
     2461 gcttagccag tcccaattaa tctacttcag gtcgagacac ccctaaagta gttccctgca
     2521 taatgacgtc cagtcttagt cggcaggagg actaagaggg acgagcagca actaggctgc
     2581 tatcgcgaaa ttatcgtctg cgtttatttt aaagttcccc agtttttacg tgacccagag
     2641 taacacgaca tgcagcctca agttccaaac ccacgtcgaa acctttacgc ccccttgagt
     2701 agtttatagt ccagtagact tttcgcaaac tttatcacca tttttattaa atttttaatg
     2761 tatatataat ataatacttt taagaataaa aatcaatgtt atgttctaga taataaagaa
     2821 ttttattcta aaaaatacag ttttatctag tttaatactt acctgtcatt tctctttcta
     2881 tacgtctttg tgcatctttc ttagccatat cttgacgttt atcatatagt tttttacctt
     2941 ttgctaaggc aatctctact ttaactttac cgttttttag atacaaacta agtggtataa
     3001 gtgaataccc ctttatagta gttgctccaa taagctttct tatttcatgc ttatgaagta
     3061 atagttttct aactcttaaa gaatcaacat tgaatatatt tccttgctca tatggactta
     3121 tatgaacttg ttttaaaaat acttcgctat tatctacaga tgcaaatcca tcacttaggt
     3181 taactttgcc ttgtcttatt gattttactt ctgttccttt taattcaatt ccacattcat
     3241 aagtttcttc tataaaatat tcatgtcttg ctcttttgtt tgttgctaat atttttttac
     3301 cagccatata aatctcacct tctttgctag aaatttattc cataaatatt agaaatttac
     3361 taaatattta atgttgccaa attatttgac taacttgtat gataacatat ttaggcattt
     3421 tagtcaatat ttcactgata tattaagtta gttaggctat atataattaa gtaatataaa
     3481 tgtatgaaaa tagctaaaaa actacttcga atatacttaa tactaaacta actctaacca
     3541 gtaatactaa tttataaatt tcaaaacaaa aatattacat attata
//
LOCUS       DAMJZE010000261         3571 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJZE010000261 DAMJZE010000000
VERSION     DAMJZE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.265"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            17..670
                     /locus_tag="PC541_003655"
     CDS             17..670
                     /locus_tag="PC541_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2841072.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            811..2082
                     /gene="brnQ"
                     /locus_tag="PC541_003656"
     CDS             811..2082
                     /gene="brnQ"
                     /locus_tag="PC541_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2841073.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2284..3471
                     /locus_tag="PC541_003657"
     CDS             2284..3471
                     /locus_tag="PC541_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2841074.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1303 a          396 c          663 g         1209 t
ORIGIN      
        1 tatagtggag gggaaaatga ctaaaattat taaagtgcaa aaacctaaat ttacaggaga
       61 attagaaatt attgaaaatc ttgaagatat acttgaagat atatttaatg atgaaaaaat
      121 atttaataaa attatagaaa atgaagttat aagtgattta gattctgtaa gagttagctt
      181 tgaatcttgt atatttagaa atgtgtcttt tgaaaattgt aatttaaaaa agattgattt
      241 acttgatgtg gtatttgaaa actgcaattt atcaaataca ttttttgatg gaggaagtat
      301 atacagagtt gaatttaaaa atagtaaatt gataggagtt agatttgatg attgtatact
      361 taaaaacgtt ttgttcaagg atgttttagg taagtattct aacttttcat tctctaaatt
      421 taagatagtt tctatcttag attcaaattt tgaaaattct gtatttcaag aagtaaaagg
      481 tgacaatcta gtatttgagt atacaaattt aaaaaaagca aactttaatg gaactaaact
      541 ttctggcata gattttacta ctaataatat tgagggaatt gagattggta tagatgatat
      601 tagaggagca atttttgatg tgtcacaagc aattgattta acgaaattga tgggaattat
      661 cataaaatga aaaaaatatt cataaaataa attgggaaaa cgttaacaat gtgtgaattt
      721 ttaccagatt gacatttttc tgaaaataaa gtaagattat cttaatattc aaaatatttt
      781 caaagggggt aatttagtgt gagtaagcag atgagagata tttttatagt tggttttgct
      841 ttattttcta tgttctttgg tgcaggaaat cttatgtttc ctccattttt agggatggaa
      901 tccggcaaag actggttaat tcctcttatt ggatttgttg ttgcagatgc tggtatagca
      961 atgcttgtta ttatagcaac agcaagatgt aagggaagca tggatgatgt acttaggaga
     1021 gctggtaaag ggttagcaag agtaatgagt gtagctgctc tagcatgttt agcacttctt
     1081 gtaataccaa gaacttgcgc aacaacatat gaaatgggta ttatgcctat atttggagac
     1141 catggtccag tagctaaagc tgtattttca ataatcttct ttgggctaac tttagcattt
     1201 actattaggt cttctaaggt tatagacata ataggtaagt atttaactcc agcgttatta
     1261 ttagtattat ttgttttaat agtaaagggt ataatttctc cagcaggacc tatgagtcct
     1321 gagcatatga tagataaaaa tctatttggt gaaggtatat cacaaggtta tcaaacaatg
     1381 gacgctctag gtgcagcatc tatggcaact ataatgttga tgtctataat agctaaagga
     1441 tatacatcag ataaagacca aataggaatg actattaaag ctggttttgt agcctgtgta
     1501 tttctagcag tagtatatgg tggtcttact tatttaggtg caacagtttc aactttatat
     1561 gatactagta tacctcaagc atctttatta gttgaaataa ctagtatatt gttatatggt
     1621 caagtaggaa aggtaatttt aggtgtaatt gtggcacttg cctgtctgac tacttctaca
     1681 ggtttaacag catcaatatc aacttatttt gaaggaataa gtaaggcgag ttataaacaa
     1741 ctggttatag gtatatgtgt agctagtatg cttatatcaa atttaggtgt tgattctata
     1801 atagccatat cagttccaat cctacaaact atatatcctg ttttattagc aatagttgtt
     1861 atggagttgg ctggtaaaca tataaagaat gataatgctt ttaaaggtgc agcatatgtt
     1921 acattagtga ttagtttatt atcagctata aatggaatga caggagctgt tccatttata
     1981 caaagcttgc cacttgcagg tttgggcttt aactgggtaa taccagctat attgggtgga
     2041 atagttggta actttataaa aagcaacaaa caagtagcat aataatttgt aattaattta
     2101 attttataat atattaattt ttagtactat gggtaggttt aatctaaacc taatctaccc
     2161 atagtacttt ttagattgga ttataatagc atgtatataa ttttttataa tataattttt
     2221 ctttgaagta tgataaaatt agtaatagtg ataagaatgt acaaatttag gaggttttaa
     2281 ttaatgctta gaaaaataac agcatttatg ctatccatat gtttaattat tggacttaca
     2341 ggttgtagtt tacttgtagg caagaaagat aaagagaaaa ttatagatga ttttaaagtt
     2401 gcagtggtaa ctcaaccatt atctgaaaat aaagtacaat acaatatggt ggaagagatg
     2461 gcaaaagaat atgaggaaga gaataaaatt gataaagaca aagatggtca aactaaagtt
     2521 aaacaaacta taaagcatgt tgtattgcca gagaatttta cttctaatat agacagtgct
     2581 attaataaga tagtaaagtt ggctgacgat aaagaagttc aggctattgt agtttcaact
     2641 gaccaagcag ggcttttacc tgctttacaa aaggtaaagg aaaagcgtcc agaaataata
     2701 actatttctg cccctatggg agatgataaa aaccaattaa gccaatttgt tgatgtgaat
     2761 ctaggggtta gtgcagagga aagaggaaaa gtattagctg aacgttcaaa agaaatggga
     2821 gcaaaagctt ttattcatta tgcctctaca gatgatttga aagatgttaa tatagccaaa
     2881 agattagaaa tgattaaaga gacttgcaaa aatataggat tgccatttgt acaagttaat
     2941 actccaaata taaatactga agaagacaaa aacaaagtta agcagttttt aaatgaagat
     3001 attgaaaaac aagttaaaaa atatggaaaa gatataaatg tatttggtgt aaatgagtat
     3061 atggatgaag ttatattgac taaggcttta gaattaaagt atatagttgc agagcaaagc
     3121 aatccatcac caatacaaac atatcctagt gttatgggtc taaaaatatc tgaaaaagat
     3181 gctcaaaatt atgataaaat aaacgacatg ataagtgaaa aggctaaggc ttttggcatg
     3241 tcaaatagac ttggtggata tcctatgcca atggatgctt ttctaccatc agtagcaatt
     3301 tatcttgcta ctgaaatggt gaaacaagat ttaacacaag aagatgtatg tgacccagat
     3361 tatttagagg cgtttacaga attaagattt ggaatcggtt cagaatttac tcctttaaca
     3421 gaggtacttt ataattatca atcagtaatt ttaagccaat taatttatta atattaagga
     3481 cgtgtctcaa aatgaacttt ttagtttatg aggcactctt tttctgttaa atcaaatata
     3541 tttttatctt tttaacaatt aaaaagaagc t
//
LOCUS       DAMJZE010000262         3570 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJZE010000262 DAMJZE010000000
VERSION     DAMJZE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.266"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            93..1589
                     /locus_tag="PC541_003658"
     CDS             93..1589
                     /locus_tag="PC541_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2841075.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            1723..2649
                     /locus_tag="PC541_003659"
     CDS             1723..2649
                     /locus_tag="PC541_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2841076.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            2767..2958
                     /locus_tag="PC541_003660"
     CDS             2767..2958
                     /locus_tag="PC541_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841077.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            3181..3531
                     /locus_tag="PC541_003661"
     CDS             3181..3531
                     /locus_tag="PC541_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841078.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         1427 a          347 c          677 g         1119 t
ORIGIN      
        1 tgttataata atttatagaa ataaataaaa aatacaatat acataatagt agaaatatag
       61 caaaaaggtg tgatttatag ggaggattac ttatgtttga atttgaaacg caactaaaaa
      121 ggttaaaaca tgaagtttta acagcagtag caagactatc tattgaagaa aatttaacaa
      181 aagaaaatat agaaaaaatt ccatatgaaa taattaaagg agaaaaggcg agatatagat
      241 gttgtgttta taaagagaga gaagtagttc ttgaaagggc gcaacttgca ttaagtttaa
      301 ctccaaatag taaatatgga gatataaatc cagattcagt tagtttggac acagatgagc
      361 aaattatata tatagttgaa gcagcatgtg atagatgtcc aataaatgaa tttacagtta
      421 cagagctttg tagagggtgt ttggctcata gatgtaagga agcatgtaaa tttggggcaa
      481 tttcttatat aaatgggaga gcatatataa atcatgattt atgtaaggct tgtggaatgt
      541 gcaaaagttc atgtcaatat gatgctatat cagaagttgt aagaccttgt aaaagcgttt
      601 gtccaacagg agctttggat tttaatagaa acactatgaa agcaatgata catgaagata
      661 attgtataaa ttgtggagca tgtatgtcag catgtccttt tggagcaata tctgataaga
      721 gtttgatagc acctgttgca aaaaaacttg caaagaaaga aaatatgtat gcaatagtag
      781 ctccagctat aacaggtcaa atagatagta atattacata tggtcagtta aaaaatgcta
      841 taaaatcact gggatttgta gatatgatag aagcttcttg tggagcagat gcagttacag
      901 ttcatgaaag caatgaactt attgaaagaa taaaatcagg agataattac atgacaaatt
      961 catgttgtcc gggatttttt aagtatatag agaagatgtt cccagatgaa aaagatagaa
     1021 tatcatctac agtgtctcct atggtagcaa caggaagatt agtaaaaaaa atagattctg
     1081 atgctaaagt tgtatttata ggtccatgta cagctaaaaa gagtgaggca acagagaaaa
     1141 gtttaaaaga ttcagtagat tatgtactta cttttgaaga actaatggca ttatttgaag
     1201 catttaatat agatttatca aaatgttcaa atgaagatat aaatgatggt tcgatttttg
     1261 gaaggggatt tggagcatct ggtggacttg ccaaatcagt acagaactat attgctgaaa
     1321 aggggcttag ttttgatttt gaacctataa aagtatctgg tccaagacaa attaaaaaga
     1381 caatgacaat ggctaaattg ggaagattag aaggtaattt tatagaagga atgatgtgtg
     1441 aagaaggttg tattaatgga gccggtaagt ttttatcagg tgtaaaagcc aaaaatactt
     1501 ttgatagaaa aaatggacaa tctactaaaa agagtgtttt atctaatgat aagattaaag
     1561 aatttgaaga tgtaaattta gaaagataat tgttttggta cagtcatttt tttattatgc
     1621 cacaaatatg cctaaaattt gcccttttta tatgtgtatt ttacagagtt aaggttgcta
     1681 tactataaat atgtaataaa taaaatttat aggagctttt ggatgaggaa aatagtatta
     1741 aaagatatcc tgtttttagt tactacatta tctataatag taatattatt gcttgtaaca
     1801 aatataacta ctaatagtaa gataaaatca acaagtgaat cttatttaaa aagtgacaaa
     1861 aatagtgtga ggcaaaatgg tatccagagg agcacaaaga gtagcacaaa aaatactgca
     1921 tttaagacta ctatactaaa cactggaaag tctgattgca tattggttga gataggtaac
     1981 aaagtcataa tgattgatac aggagaagat aaaaatggaa aacaaatagt ggataggtta
     2041 aaagaaaaag gaataaatac acttgactat ttgattttaa ctcatttaga taaggaccat
     2101 ataggtggag tagatagtgt gttatctagt gttaaagtaa agaatattat acaagcaaat
     2161 tataagaaag atagtaagca atatgatgaa tatattgact cacttaaaaa agctgatata
     2221 gaaccagtgc tactaaaaga taatatgaat attgtaatca atggtgcaga aataaacata
     2281 catccggctt caaaaagcaa gtacgagagt tcaaatgatt attcaataat cactaatata
     2341 agctatggag cacataagtt tttatttgca ggagatgcag aggaagaaag attgtcagag
     2401 tttataaatg gaaatacttt aaaatatgat tttgttaaaa tgcctcatca tggaagatat
     2461 gacaaattaa ctgaaacatt tttagagtct atttcacctc aatatgctgt tattacatgt
     2521 tcagagaaaa aagaaccaga ggaaggtgtt ttaaaaatat tggaaagatt gaatataaaa
     2581 acatttttga cttctaatgg agaggtagtt atcaatagtg atggtaagac attgagtgtg
     2641 aatcaataat ttttttaaaa atatctaaaa aaatttaaaa aaatcattag caaatattac
     2701 cccttaaaca tagtatattt taggagctga aactccacat acaaaatgac taggaggtaa
     2761 catattatgt tggatagatt ttttggtgaa ggcggatttg gtggatgtgc ttggtggata
     2821 ataatattat tcttcttatt cttagcattc caagattgtt gggctgaaat ggacttatgt
     2881 gcatggatac ctttcttaat attattatta atactttgtg aatgtggtga cgtttttgat
     2941 ggaggatgtg gatgctaaaa aaatcaattt agatgattta ataaagctta tggtgggtat
     3001 aaataaaata ccaaaccaat cacaatctag tgtttgaagg tagtcggaaa tcccccgcta
     3061 ccttcttttt tttattgtta aaatttattt ttgaattatt tcatttacat ataataattt
     3121 ttacgataaa gtctgatata attaaaaaga aaataaaatt tatttatagg agatgtaaaa
     3181 atggctatag tggataagat tttcaatgag ataaaactta tggttgcaag acttttattg
     3241 ggaaaaaaat acagtgaata tgaagaaaaa gatttagtgt ataatacaga agaagaagtt
     3301 atacttataa ctttaaaaag attagttttt cagggaaatg taaacgaagc agaagaaatt
     3361 ttatttgata aagctaagag tgtaaatagt gaaaatatgc agtatatagc aatagaattt
     3421 tatactatgc ttatggaaaa gactgatgaa gaattagaag ctatgaattt ttctaaacaa
     3481 gaagtttatc aaggtataga agatattaga aaagtattga atttagaata aaagatatat
     3541 gaaaatattg aaataaatta atgctaaggt
//
LOCUS       DAMJZE010000263         3536 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJZE010000263 DAMJZE010000000
VERSION     DAMJZE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.267"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(102..293)
                     /locus_tag="PC541_003662"
     CDS             complement(102..293)
                     /locus_tag="PC541_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841079.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            586..804
                     /locus_tag="PC541_003663"
     CDS             586..804
                     /locus_tag="PC541_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2841080.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            1113..2732
                     /locus_tag="PC541_003664"
     CDS             1113..2732
                     /locus_tag="PC541_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2841081.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            3106..3300
                     /locus_tag="PC541_003665"
     CDS             3106..3300
                     /locus_tag="PC541_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2841082.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT         1341 a          412 c          448 g         1335 t
ORIGIN      
        1 aaaaattaaa taaataaaaa ttttataatt ttatatttat caaaaaaaga ggcttgtatt
       61 tattttataa tcatacaaat aaatataaac ctcttttatg tttatctatg ttttaaatac
      121 acaaaatatt cttctacttc atctccattt tttactttta ctaaatattc attataatga
      181 gcattatatg gctgtgtcat tttgcattct attacatttt ctatgtcttc taattctaaa
      241 agttctacat cctttaaaaa ttttctttct atgagttcca ttaatctttc cataatatat
      301 cctcacttcc cctataatta agataaacta tatatcctct taatacagag tttatctgtt
      361 tattatatta tatccaatat attacagttt ctaacatcat tcataaatta ttttaacaaa
      421 tagagattga ttttttaata aatatgcctt gataaaatga aacatagacc aactcttagt
      481 tttatgtaaa gaaaacttaa agaaaactat agattatatt cacaaaaatt actcagaaga
      541 tataacagta agtaatatat gtaatgaatt acaacttaat aaaagttgtt tttttcttta
      601 tttaagaaaa aaagtggata tacttttact aattttttaa gtaaagttag agttgaaaaa
      661 agcaaaaagt atatattaaa aaaagacttg tctattttag acgttgctgt actaattgga
      721 ttcaatagtc aaaattacta tagtataatt tttaaaaagt ttaataactt tacaccaatt
      781 gaatataaaa atatttataa ctaaaaaata tcattctaaa aggatgttat ttttttacat
      841 attttttaca attatagtta attctttgtt aaattgattg tacataaaat actaaaaatc
      901 taagttcaag tatttaaatt gctatatatc ataaattttc taaaattaac aacaacttaa
      961 aaatttaaca ttctcttaac gtatacttaa caaaacatta atatttttat tgacaaaatt
     1021 tgttattcta tattatggaa acaataaatt ttagttaata aagtttataa ttcagataaa
     1081 aaaattatta atttcttaag ggaggaaata tcgtgaatat agtaattagc cttatcggtg
     1141 gacttggact ttttctttac ggaatgagtt taatgggaga aggtttacaa aaatcagcag
     1201 gagacaaatt aaaaaaaatt attgaactct taacaagtaa tgttgttatg ggtgtactgg
     1261 taggtacagt cgttacaggt attatacaaa gttcaagtgc tactactgtt atggttgtag
     1321 gatttgtaaa tgcaggtata atgaatctaa gtcaagctat tggtgtaata atgggggcaa
     1381 atataggtac tacagttact gctcaattag tttcatttaa tcttgaagga attgcaccaa
     1441 ttgctttagg tataggtatt ttattctatt tatttacatc aaatcaaaaa acaaagcatt
     1501 tatctgagat acttataggt ttcggtatat tatttacagg aatggagttt atgaaagatg
     1561 cagtagctcc acttgctgaa tataaagcat tcactgatgc acttttatat tttagtaaaa
     1621 atcctgtatt aggtatattg gcaggatttg ccataactgg tattgttcaa agttcaagtg
     1681 catctatggg tatgttaata gcactagctt ctcaaggaat acttcctcta tcatctgctt
     1741 tacctattct ttatggagat aatataggta cttgtgttac ttcactttta tcaagtgttg
     1801 gtgcaagtag aaatgctcga cgtgcagctg taatgcattt aagttttaat gtaattggta
     1861 caataatatt tatgcttgtt ttaaataaac ctatatcagc catagttact cattttgacc
     1921 caactgatac agctagacaa atagcaaatg cacatacttt atttaatctt acaaatgtta
     1981 taatattatt accattttct aaatacatag ttaaattagc aaatagactt atacctatta
     2041 aagaaactga atcagaaata gtaaataata ctaagtactt agatgaaagg atgtttagca
     2101 ctccatctat agcacttggt aatactgtac aagaagttgt aagaatgggt cataaagcta
     2161 caaactcttt ggaacattca attgcaggat ttttaaataa gagcaatgaa gatattaata
     2221 aagcttttga gtcagaaaaa gttgtcaata agttacagaa agatatattg aactatttgt
     2281 taaaactttc taaagaacct cttagagatg atgaacgctt tagaacagat ttgcttttta
     2341 atactgtaaa tgacattgaa agagtctctg accatgcaga aaatatagct gaattggcca
     2401 tgagtgttaa ggaaatgaac attagttttt ctgatagtgc gataagagaa atatacgaaa
     2461 tatacaataa gacaattact aattttaagg atgccttaat agttttagat gttaaagatt
     2521 ttgagttagc aaataaagtt ttagaagttg aaaatgaagt aaattattta gaaaaaactt
     2581 ttagaaattc acatatgatt agactaaata atggaagttg tacaattgat gctggtgtac
     2641 tttatcttga cttacttact aatctagaaa gaatttctga ccactcaaca aatatagtaa
     2701 aacaagtact aaaacttaaa caaaaaatat agtgattatt tttatatata atcttaatca
     2761 atgagaagta agaagctata gataatgctt atatcattta tggcttctta ttttttatgt
     2821 aatactcaaa tttttatagt attctcttat tattaaacag tttctgccta gaatattcca
     2881 tatatatatg acttctttca taaaacttga actgcccata aataaagtca caagtataca
     2941 taactatttt ttaatcaatt ataaccaata tttgttttat ttatcttcat tttaattctt
     3001 cattcaataa tgtaataata ttatttttta tttggatact attatatcat ctaaatatgt
     3061 tattatatat ttggatattt tcatattaat aggagggatt ctacaatgat aaattatatg
     3121 gtagaaatta tatatagcga aaaaataagc gaagtagaaa acatgttgaa aattgaaaat
     3181 atacaatatg aatttacttc tcgtgatggc tttatatttt ttggttttta tgatatgaca
     3241 gacgcatata gatttgagac tttatgttca gatgaaaaaa ttcgatttaa aagaatataa
     3301 tagataaaaa agtacatatt tctaaactat ttgtttttca aatttaggtt gtgttttaat
     3361 gtagtgttga tattataaac aattttaata ataaaaaagt agggacatta tattaatatg
     3421 ctcctacttt ttactcatac ttattaatat tatttatcaa aattacccta caaactggaa
     3481 tttatcattc tttcataatt ttaaactatc atatttaaaa tatataaaat tctaca
//
LOCUS       DAMJZE010000264         3514 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJZE010000264 DAMJZE010000000
VERSION     DAMJZE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.268"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(227..610)
                     /locus_tag="PC541_003666"
                     /pseudo
     CDS             complement(227..610)
                     /locus_tag="PC541_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      836..933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            988..1164
                     /locus_tag="PC541_003667"
     CDS             988..1164
                     /locus_tag="PC541_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841083.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1390..1590
                     /locus_tag="PC541_003668"
     CDS             1390..1590
                     /locus_tag="PC541_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2841084.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2342..2524
                     /locus_tag="PC541_003669"
     CDS             2342..2524
                     /locus_tag="PC541_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2841085.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            2595..3440
                     /locus_tag="PC541_003670"
     CDS             2595..3440
                     /locus_tag="PC541_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2841086.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
BASE COUNT         1341 a          417 c          427 g         1329 t
ORIGIN      
        1 tagtcaatat aaaagacagt aatttatcta tagtgttact tatattttat atatatggct
       61 atttttcaac attttacaat atataacatt tattttttta acttttgtca gtaaaatgtc
      121 agcaaaacac tttatccaca atttttatat aagttttcca caacttatca ccaatattat
      181 ccacataaaa aaggtagcac ttatattatt agctaccttt tatttattaa tctttatatt
      241 tccagctttt tatccaactt cctatttcta ttattttttc ttcagttatt tcttctccat
      301 catgtaattt tgaaattaat tctgataaag gattgttatg taaacctcta aaaactcttt
      361 ttccctctat tttgagatat tctctctctt ttattaatac tgtatagtgt acatgcctcc
      421 caattctcta tgatattaaa aaaccttttt tggttaatct agctaaaagt ataattgtag
      481 ttgtatgctt ccaaccatat gttttttcta tttcatctgc aacatctttt gatattacag
      541 ttttataacc agtatgccat atgcatttca taatttttaa ttctgtatct ggtaattttt
      601 tatttagcat aatatattcc ttctttcaat ttatgtcact tcttcaaatt tatctagctt
      661 aaattataat aggagctttt tagaacaaaa actatattta atttttacca ggtactctac
      721 gtgtttagtc tgtttttaag tgtaaaataa tgttcatatc tataaattta tttaactttc
      781 aaacaatcct gttgaataat aactaattct attgtattat tttagttgta gaataaaact
      841 aaatcggcaa aactagagaa atttagtgac gcaaagctat agggactaag acttatataa
      901 atatcttatg agttatgtca gccagttgcc aaagagatag tgttcttttt gtttttatga
      961 aagtttttta gggggataat aatttatatg tttggaaata aaatggataa atgtacacat
     1021 gtattgactg cttatattag cagttcgtat gattattgta attttttaga tacacagtta
     1081 gatgatttta tattagagta cggagaaaat gtagtagagt cttgtttaca ccaagtaatg
     1141 gtattggtaa gtaagtataa ttagatagac attgatttct agaaaaccct ttttagtttt
     1201 atctacattt atattaatat atagcaatgt aaaacgttta aatatatttt taatattaag
     1261 aataattaaa atgttaacta aaaaatataa ttaagtagac atttctatta atctatgata
     1321 caatagtatt tgtggtaata cacaattaaa tcaagtatat cttgatatag gttaatagga
     1381 gtatttttaa tgaataatgg aatagtgaaa tggtttaata atgaaaaagg attcggtttt
     1441 atatcaatgg aaggtagaga tgatgtgttt gctcatttct cagctataca aacttcagga
     1501 tttaaatcat tagaagaagg tcaacaagta agctttgata tagttaaagg tgctagaggt
     1561 cctcaagcag aaaatataac tatattataa tatatagatt tttcgggaaa gactcttttt
     1621 agagtctttt tttattgtta atatatgtaa gataatcatt ggtgaagtaa ataaggtata
     1681 ttgagtaatg ttcttgatag tttatatgct aatgaatttc aaaaaaataa gcactcctat
     1741 aaaaagagtg ctcttaatgt aaatataaac cttcattggt ccagcctaat accccgtatt
     1801 ctccaagttc taactatttt ttatatacat tatcactttc atccatatct aacacaatta
     1861 tttcttcttt agaatcaatt tgtaaaatat gaggactgtg tgttgtgaca attatttgtg
     1921 tttttaggag aattgctttt aatgtaataa aaattttaca taccacatag ttgatataaa
     1981 actataataa ttacataaga ttgtaataat aagacttatc tttacatacc acatagttga
     2041 tataaaacaa tgaaattaac atacaagaga ctaagacaat taaaatcttt acataccaca
     2101 tagttgatat aaaaccccaa aataaactta gcatctccaa tgtttacaca tatacaatgc
     2161 tcttaaaatt gcagtgaacg cgcaatagtg caattgataa tacttattat atattctcaa
     2221 tgccttatat ttcaactgct aaacctcaat ttgtcacaaa tatcgctcac tgcaaaattt
     2281 ctatattttt attatatcat aacatataat taatattttc aattaatttt tacatttaat
     2341 tatgtttaaa ttctataata atgcaacttt aatgtttctt tctggaacta atataaaaac
     2401 tatttcagaa agattaggtc atgcagatat aaaaataact atgaatagct actctcagtt
     2461 tttagaagag atggacagag aagcatttga aaatttgagc aaaatactat ttattatatt
     2521 ttaaagaaga tgacttattc aatatttgat gctagaatat aattaataaa aatttaatta
     2581 taggtgattg tattatgaat attattaaaa gcataacttt actcttaaca ttattaattg
     2641 tgatattaat gacaggctgt tccccaagag aaagtgctag tgaagttgca aaaaaatata
     2701 ttgataagat gaactctggt gactatgaaa cagcatatag tttattaagt aaagatagta
     2761 aaaaaaaaat tagtatagaa aatcttgaag aatttcaaaa tattttagtt agtacaaaaa
     2821 gaataattag ttataaaata ggtaaagaaa aaatcataaa aaaatataaa catgatggaa
     2881 aagaatataa aaatgttgtt aaattagaag aaacttttaa tataaaaaac cttctttata
     2941 aaaaagatga ttctctaaaa gtaaatagat attttgtagt tgaaaataac aaatacaagc
     3001 tattattgta tgaaagtttc aaaaaagaat ttggtattaa tagtatggac cttgctcaac
     3061 ttaagcttaa tgattttgga ctaaaatact ttaaagaagt tgaccttata ctaaaaaaag
     3121 ctattgctat taatcctact gattcaaagc tttattatgc ccaagcatgt aattatatga
     3181 attttgatgg atttcctggt tattcagata caacatataa tgctttagat tcaattaata
     3241 aatctttaaa gtatttagat aaaaatgatt ctgactacaa aaaaaatgca agtaatatat
     3301 ataacttaaa aggagttatt cttatgtttc acaaaagatt tgatgaagca caacaatatt
     3361 taaataaagc cttatacttt aacaaagata atgaagaccc aaaatataat ctaaatacaa
     3421 taaaaaaaca tctaaactag atatttaatt tttgaattta taaaatattt ttttatgtat
     3481 aattcagcac tgaatcataa tttctactaa acta
//
LOCUS       DAMJZE010000265         3512 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJZE010000265 DAMJZE010000000
VERSION     DAMJZE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.269"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(179..1195)
                     /locus_tag="PC541_003671"
     CDS             complement(179..1195)
                     /locus_tag="PC541_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2841087.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1378..1740)
                     /gene="spoVAE"
                     /locus_tag="PC541_003672"
     CDS             complement(1378..1740)
                     /gene="spoVAE"
                     /locus_tag="PC541_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2841088.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1754..2767)
                     /gene="spoVAD"
                     /locus_tag="PC541_003673"
     CDS             complement(1754..2767)
                     /gene="spoVAD"
                     /locus_tag="PC541_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2841089.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2934..3371)
                     /gene="spoVAC"
                     /locus_tag="PC541_003674"
     CDS             complement(2934..3371)
                     /gene="spoVAC"
                     /locus_tag="PC541_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2841090.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1135 a          636 c          384 g         1357 t
ORIGIN      
        1 ggattttcaa ataattgaat aaaattattc attcatatta taaaaactat ctcaataaat
       61 aaaggagtac aatgtactta tatacaccta ctccttttaa tttttattta tattatttgt
      121 taaaaaactt cacataatct ataatatttt taataaacgt ttgatactta aaagcttact
      181 actttattct tttaacgcta tttcgtatta ttaaccttgg atataatttt cttggaatgt
      241 gttctgtact ctttttattt attatattta ctaaactatc tactccattt tttataactt
      301 gctctcttgg gtcatctatt gttgataaat gcgacctaaa atatgatatt aattcaatat
      361 tattataccc tattatggaa atatcttctg gtacatttat accataagta gtttttaact
      421 cttgtattat agaaatcgct ggtacatctt gttgtacaaa tatagctgaa gcttttttta
      481 ttttatcaat gtatttttct aaaaattcaa cttgagattc aaaatccatt ttagatttaa
      541 tgacttctgt ctttaaattt gcttcttcca tagcattcaa atatcccttg gttctatctt
      601 catgcatttt tgaactctta tcatctgaag ttactgttat tatatcttta tgaccatggt
      661 caattaaatg cttggtagct atataacctc catacacgtt atcgtcccaa aagaaattat
      721 ctacttctcc tataaaagat ggtttaaaat atataaatac atgtggaaaa ttattgtctt
      781 ttaaaaaatc atactcttct ttttttattg attgagacac aattactaat ccgtcaacca
      841 tttgtccatt taccatttta tatacattac tatcaccact aatgttatta ttaggctgta
      901 ttataaaatc gtatttatta gtctcaatag aatttataga actatccatt atatcactaa
      961 aaaactttct agtatcttga tgattaaaat tatttgaaaa gattacccct atcctgttag
     1021 tttctttttt tgctaaattt cttgcattta cattaaaatg gaaccctagt ctatttgcct
     1081 cttctaccac tttctttttt gttttttcag atacattagg attattgttt aagcacctag
     1141 aaacagttga ataactgata cctagattcc ttgcaatatc tttaatagtt accatcttta
     1201 tcccctactt cacttcattt tattttgata atataatact acactatgtt gtaaataatt
     1261 gtcaacttca attatgtaaa cgcttcttta tataaaaaac aggtggaatc taaaagatat
     1321 ccacctgctt tataaaaagt tattatttca aaaacttatt attcttttaa taaagattta
     1381 tggttttgct tttggagtaa atactactga cataatatat ccaaagaata tagctgctgc
     1441 tattccacca gctgtagcag ttgtacctcc taaaaaaata cctaaaaaac catcactttg
     1501 aattgatttt attactcctg tagcaagtga atatccaaat cctattatag gaactgttgc
     1561 acctgctgct gcaaacttaa ctaatggctc ataaagtcct agaaaagtta aaactacacc
     1621 agttgttaca tacgttacca taacatatgg agtttgcatt ttgaaataat ccatcattac
     1681 ttgggctatt aaacatataa ttccacctac aataaatact cttacatagt ccattaacat
     1741 attatatcac ttcctactca tttactatta ctactgcatg tgcaacacat ggtattgtct
     1801 gcttttgtag tgtacttgtc ggtgaaagta gtgctcctgt tgatacaagc attactttgt
     1861 taaattcttt ttttctcaac ttatcatata tatatcctgc aaacactgta gctgaacacc
     1921 cacatccact tccaccacag tggacatctt gttctttaag gttaaacatc tctataccac
     1981 agtcagtata aacttttgat atatctacac ctttttcttt tagaaagtct tctgctatct
     2041 gttttccaag agtacctaaa tctccagtag caattatatc aaagctattt gggtcatcat
     2101 tagtatcttc aaagtaagag tagatagtat ctattgctgc tggagccata gctggtccca
     2161 tattattacc atcatcaata ccctcatcta ttactttacc aacagtaaca tactttactt
     2221 ttggaccgtc tccattagga gctaataata cacttcctgc tcctgtaact gtccactggg
     2281 ctgtcattgg tttttgattt ccaagttcaa gtgggaatct aaattgtctt tctgctgtac
     2341 aatagtgact tgatgtacct gacagtacta aatcagcaaa accaccatct ataagcatag
     2401 aacctataca aagaccttct gccattgtag aacaagctcc atatatacct aaaaatggaa
     2461 tagccagttc tcttgctgca aaggatgctg gaactatttg atttattaaa tcgccaccta
     2521 gcatataatt tacatctgac aattttttac ctgccttttg gatagcttgt tgagatgcag
     2581 tctctaccat tttactttca gctaattccc aagtcttttc accatataaa tcgtcagtca
     2641 taatcatatc aaaatagtcc tttagtggtc cttcaccttc cttaggacct actatagttc
     2701 cagttgatat tatggttggt ttattttcta acttgactgt tctttttcca attcttttat
     2761 ttttcatatt ttcacacctt tttaattttg cattttacta ttcaaatttt taatattaat
     2821 taaaaatatt aattaaatct gcagaattat ttttcaatta gttactttag tagctatttt
     2881 cagattttat accttcttca taattaataa aatttaaatt aaataaaaat catctaaaac
     2941 atcttaaaaa tataataaat aattccacat aaaatagaag aacctatccc ataaactaaa
     3001 actggtcctg caattttaaa taagtttgca cctacaccta atacatagcc ttctctttta
     3061 tactccattg ctggagatac tattgagtta gcaaaacctg ttattggaat tatagaacca
     3121 gctccagctc tttttcctat taggtcgtaa actcctaatc cagttaaaaa tgcccctata
     3181 aatattaatg ttattgatgt tgcagaacta gcacctagtt tatcaagacc tccaactttc
     3241 atatacaaat catttattgc ttgccctatc atacaaataa ttccacctac aataaatgca
     3301 agtgtatagt tttttaaata tgtaggtttt gggcttattt ggtctacata ttttttataa
     3361 tttttatcca tactaaacac ctctctaaag tattatttgt taattttaaa aaaataaacc
     3421 tattatttat tttgactatc taaagtttgg tcaaaaaata aaaaagatgc ctaataaaga
     3481 aaaaatctat ttttccataa agacaccttg tt
//
LOCUS       DAMJZE010000266         3468 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJZE010000266 DAMJZE010000000
VERSION     DAMJZE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.270"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(122..1689)
                     /locus_tag="PC541_003675"
                     /pseudo
     CDS             complement(122..1689)
                     /locus_tag="PC541_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1664..1807)
                     /locus_tag="PC541_003676"
     CDS             complement(1664..1807)
                     /locus_tag="PC541_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841091.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(2025..2207)
                     /locus_tag="PC541_003677"
     CDS             complement(2025..2207)
                     /locus_tag="PC541_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841092.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(2596..3342)
                     /gene="cdtR"
                     /locus_tag="PC541_003678"
     CDS             complement(2596..3342)
                     /gene="cdtR"
                     /locus_tag="PC541_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2841093.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1158 a          451 c          379 g         1480 t
ORIGIN      
        1 aaaaagaata tattagaaga agataatatt taaataaaaa ataccttata aagttaattt
       61 tttaatactt tataaggtaa atacatattg tattgtgggg ataaatttaa atcatttgat
      121 actaatcaac actaagaacc aataactctc tatcatcttg agtaattgta tatattctta
      181 atttcttgta tgtcataata ttttctccac ctgtaaaata actcctaaga ttaacacaat
      241 cagttttagg ttgatttgta tctcctaaat aattacgcaa ttctttatct ttagtttcaa
      301 tatctttaaa tccaaaatat tgtaaagtag cttcaactct atatttttgg acataatcga
      361 gagcttcttt attagtttat gttggtgtaa aatacatacc atttatataa gcctttattg
      421 tacttggatt aaatttaaaa tctgcagaat atattttatg tgtaactatt atttcttgaa
      481 cagttggaat tttaacttct ggttcatcaa gtatttcagg agtactattt agctctgtaa
      541 tagataagtt atctaagtat attgtatcac tatcaattaa tgctatattt atattagaag
      601 aatctttttc agtagtttca aattcataac taaattttgt atatccttgt tctagtacca
      661 aataatccgt tttttcttct tttactttta tatttacaat tattgaatta tatgttaaag
      721 gattttttga attttactaa aaacataacg tttataaggt tttagttcag acataggtat
      781 tttaatcttc gtctcaccat ttaatctatt tgctgagcct tttaaaccat cttgatttgt
      841 agtattaaca ttactccata tactagggta attattgtaa tcatcaaaat tagtaaagta
      901 agttggtgtt tttataagta tattcattcc tctttcaagt ttaacattac atatttgttt
      961 atcagacaaa gtttttaaac tgtctttaat cttattgcct atattatcat caaatatgag
     1021 ttcaacacaa ctttcatcta ttggtatatt attaaaatat aacaaaccat cttttttagt
     1081 agtgccaaaa gttttttcaa ttgcttctcc aattgtaagt tcaggtgttt tatcttctgg
     1141 gacctgtaaa cttttagcag taattcttct tttgtaagat tcattttctg tatctaatat
     1201 aatagataca gaaatactgt caatttgacc tatatcgtct taccaactat ttcattctgt
     1261 tactatttgt ccagaaccat tttttgtacc aaaatttcca cttacttgtg ttgtttctaa
     1321 tttaatttgc tttccatcat ctaatttttt taattaatta taatttatag gaatcagtct
     1381 agaactaaat tgatacattg tgttaagagc cataggtgaa aatccttttt tgagataagt
     1441 atttccagga gatagattat tgccaatttg attttcttgt gctttgatag ttgataatgc
     1501 atctccatct aacactaaat ttgttgttgg tgtcactttg tacataggtg cagtacctgt
     1561 gttgtagtat ctaatatttg catttatata tgcagattct cctttgttta tacttaatcc
     1621 agtatttcat gattatccat tgctattttg aacagcagtt gaattatctg ttgtatggta
     1681 ataatttgtc ttgtatagtt tctttaaact cattaaattt ttctaatgaa atttcgtttt
     1741 gattttttgt tcttattact ttattaaatg caaatttttc tggagattca aaataatata
     1801 catacatagg tttatctatt ttattttttg atatagcatt tcgaagttct ctatattctt
     1861 tttctcaaga atttgcttct atttgataat cataaaaata atttcttgtc tgagaatatt
     1921 tgcttatctc tatagaatct tttttatatg atcctaatac ttctttttca gatttttcaa
     1981 gtttttgatc ttttctttct cattctttag ccttttcttt atctttaaaa aaatccttct
     2041 gatctttcta taggagcctt gtaagtagta ttacaaactt ttccactata gctttgtaaa
     2101 tcttgtgcat aaatgttatt aggtaacaaa ctacctaaag ttagatataa tatcaacaat
     2161 atagatatac aattactaat acttttatgt ttcctaaatt ttttcattta ttctccctac
     2221 aaatattagt ttagttaact tttgaatttt aatttattta gattatataa tctaaataag
     2281 aattttttat ataaatttca taaaatatgt aggtatatat cataaaaata tgtatttata
     2341 tgatatgtca gtacaaaata acaactgaat attttaaaga gactatttcg tacttttatg
     2401 atttaatgag ctatcttatc gaatcataat ctaggaacaa ttgttataat atctaatagt
     2461 agttatatat gatatagtca gtatgtgttt tttcacaata tgattattat atagaggatt
     2521 cttagggatt actctttttg tataaaaaag agatggttca aatttttatc ttgagacatc
     2581 tcctttttct atttattatg ttttaataat attctttaaa atatttctat atgtattaga
     2641 tatacgacat atatggccat tactcatttt aatattattt ttctttaggt cgagagaaac
     2701 aatatgatat ttattaacta caaaagattt atgtacacgg ataaagcatg catctaaatc
     2761 tggtaaagac tttaaacttt tatagaattc aaatattcta gaagtagtca ctaattttat
     2821 tttatgtgga atagtgcttg tttcaaaata tataacttca tcaaatgaaa ttttccatag
     2881 tgagaaatct gaatagatag taaaaaaatt agagcgataa tttttttctt taatgtatct
     2941 tttgtgagca attttaatac aagcttttat tttactttca gcaatttcca tatcatgttt
     3001 tagtataaag tccataactt ctaacctata atcgaagatc cctaaaatca tttctatatg
     3061 atttgtaaga aatatgattg gactaatata atctgattct ctaatttttt gtgctatcca
     3121 taatccatca cataattcat tacttatctt atattttgcg tctacaacta taaaatatat
     3181 tcttacttta tttttttcag taagctcttt taataacaaa tttgcattat gatataatct
     3241 tacaacatca ttatcaaaac cttcttttat taaaatatta tttatttttt cttttagttt
     3301 cattccaaaa caaacatcat tatcaaagat taatatatcc actaaatacc ctcctataaa
     3361 aaattcaaaa gtaaaaaaac aaaagtattt tatagaaaat acataaaata acactaggag
     3421 ataaataaga ttaaggatag ttagaattaa ggtatattta tgcttgtt
//
LOCUS       DAMJZE010000267         3448 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJZE010000267 DAMJZE010000000
VERSION     DAMJZE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.271"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..1114
                     /locus_tag="PC541_003679"
     CDS             <1..1114
                     /locus_tag="PC541_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2841094.1"
                     /translation="NNISIIISDESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQ
                     YKKLINYLDKSICMGALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNG
                     TLSFTGNLNSIMSVLYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHL
                     KEPVLKVVSIFTGSQLLFEDTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKP
                     LSVGRPFPGIDIYIREGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDII
                     NIGGFKVSSTKIENEVKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKM
                     KDNLMKNEIPKKIIFLNSFPYTSSEKIDRLALLKML"
     gene            1190..2065
                     /gene="dpsA"
                     /locus_tag="PC541_003680"
     CDS             1190..2065
                     /gene="dpsA"
                     /locus_tag="PC541_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2841095.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2068..2658
                     /locus_tag="PC541_003681"
     CDS             2068..2658
                     /locus_tag="PC541_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2841096.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2865..3065)
                     /locus_tag="PC541_003682"
     CDS             complement(2865..3065)
                     /locus_tag="PC541_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841097.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1323 a          414 c          513 g         1198 t
ORIGIN      
        1 aaataatatt tcaatcatta tatctgatga atctatggac gttattgatt tatatgatga
       61 taacaataat gaagtagcaa aaaaagttaa tttttctatt tttaatccta aatttaatat
      121 atatatgtat caatataaga agcttataaa ttatttagat aaaagtattt gtatgggagc
      181 tttatcttca ggttctacaa gtgttccaaa ggtattatat agaacatatg agagttgggc
      241 tggatttttt cctatacaaa atggtatttt taatatctct ggggaatcaa tcttgtttgt
      301 taatggaaca cttagtttta cagggaatct aaattctata atgtcagtat tatatgaagg
      361 aggtagtatt gtaataagtt caggtcttaa ttgtaaatca tggataagga caataaaaca
      421 atataatgta actaacatat accttatacc tacaaaactt caacttttgg ttaaacactt
      481 aaaagaacca gtactcaaag ttgtaagtat ttttacaggg tctcaattat tatttgaaga
      541 tactgcaaga aatctaaaaa aatatatgcc aaagtctgaa attattttat actatggggc
      601 aagtgaatta aattatatta catatttatg ctatgatgaa ctaattgaaa aacctctaag
      661 tgtaggcaga ccatttccag gaatagatat atatattaga gaaggaaaaa tttttgtgaa
      721 tacagaatat gcagtatata atgcaaccaa accttattct gtcaatgata ttggatatta
      781 tgataatgat ggatacttaa tatttgaagg aagaagtgat gacattataa atattggagg
      841 atttaaagtt agttctacta agattgaaaa tgaagttaaa aaaattcctc aaattgaaga
      901 tgcaattgta ttgccgtact cagacactat aagaggaaat caaattgtat tatttgtaat
      961 aactatagac aaaattacga agaaagattt gttgattaaa atgaaagata atctaatgaa
     1021 aaatgaaatc ccaaagaaaa ttatcttttt aaattcattt ccatatacct cttctgaaaa
     1081 gatagataga ttagcattat taaaaatgtt ataagtataa tatttagtaa ttttagtgaa
     1141 ccaatgtatt gataaaaaca tattgtatat agtaaaggag gcttacaata tgtcaaatca
     1201 atacgatata actgttattg ggggagacct gcgacaagtg tacatggtaa aaaaactaat
     1261 taataaaggg tattctgtag tcacttatgg gatatcaaat gaaattataa atggaatcgt
     1321 tggaaaatca acttcactag ctgaagcact atccaaaagt tccattattt tatgtccaat
     1381 tccatttaca aagaatcaag ttaatattga aaatacggat gattgtcttg atgctactat
     1441 tgataacttg ctaattaatt tatctcctaa tcaaatatta tttggaggta acataccaac
     1501 taaagtttgt aaattttgcg agcaacactc tattcagatt tatgatttta tgaaaatgaa
     1561 tgatgttgct attttaaatg cagttgctac agctgaaggg gctatagcag aagctattaa
     1621 aagaagtata atcaatatac atcaaagtaa ctgtttagta ttaggttatg gaagatgtgc
     1681 acaaatactt gcaaaaaaat tatacgcttt ggatgcaaat gtatgtgttg gcgcaagaaa
     1741 taatgatgca agaagtacgg ctaatgcatt tggatattcc agtatttctt taagtcaact
     1801 tgatgaaaaa cttttacaat ttgattatat ttttaacacc ataccagagt gtattttaac
     1861 gaaagaaagg ttagaaaaag tatcacaaga agtaactatt atagatatag cttcttctcc
     1921 tggaggagta gattattctg ttgttaaaga agcagggatt aatgcgactc tttgccttgg
     1981 gcttcctggt aaatattcac caaagacatc tggtgaaatt cttgttaacg ctatagaaaa
     2041 tattataaat gaaagaaggg attagaaatg agacttgaag gacttacaat tggagtagga
     2101 tttactggtt cgttttgtac ttatgataaa atatttatag agttagagaa tttggttaaa
     2161 gaaggagcta atgtacacac tatattttca gatgtatcac aaaatatcga ttgtagattt
     2221 ggaaattctg aagagtttat gaaaaaagca tatgaactta caggaaataa acctatagtt
     2281 acgattgaag aggcagagcc atttggacct aaaggaattg ctgatattat aataatagct
     2341 ccttgtactg gaaatactgc tgcaaaatta gcaaatggta ttacagattc accagtatta
     2401 atggcagcaa aaggtcatct tcgtaatgat aaaccactag taatatcaat atcaacgaat
     2461 gatgctctaa gctttaattt caaaaatatt ggcattctcc ttaattctaa aaatatatat
     2521 tttgtacctt ttggtcaaga taattgcaaa gcaaagccta attctatgat tgcacatact
     2581 gagctaataa ttccaactat agaacttgct ctagagaaca aacagctaca gcctgttata
     2641 aaaagtccac atcaatagaa ttggttctta taatattatg tattagatac aatactagca
     2701 aagaggctgt ctcaaaatag agagaagcct ctttttcttg ttgaaatgat gaaaatatat
     2761 ttaatttcat acaaaaaaat gcctcatgga ctaaaaagtc tattttgagc taatctccgt
     2821 aatttttgct atattaaaat tatttactta atccaatttt taaattagaa tttatatcgt
     2881 tttgactctt caaatatcaa tctaactgtt gttgatacaa aagaagagga cataattaaa
     2941 acaaatactg atagtcctct aataaacaaa ttatctacat aataagttac tattccaata
     3001 cctccaatcg ttaaaattac acttataatc aaagaaaaaa cgctaattgt ttttaatttt
     3061 tccatgctta tcctccttct tataaacttc aaatttatct atataatatt aaatatattt
     3121 tatttcatat tatataaaaa tcatgaatat atattttttt aattatacta tatgttgtaa
     3181 atgtttgtac atttaactat atttacttta acttgttaat taagttttaa ttttcatttt
     3241 caaggatttt tatttttaaa cttgatagag tatcatatta aaaattcttt ttataaagtt
     3301 ttatttgcaa aaaatacaaa aatataaacg ttaaaaaaat aacgttatcc ttaagttttt
     3361 agatgatttc attagtgatt tgcgtaaaaa ataaagtgat atctagttaa attaagtatt
     3421 tgtaaagcta gatattatta gtagaaat
//
LOCUS       DAMJZE010000268         3363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJZE010000268 DAMJZE010000000
VERSION     DAMJZE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.273"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(89..613)
                     /locus_tag="PC541_003683"
     CDS             complement(89..613)
                     /locus_tag="PC541_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841098.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            complement(603..1022)
                     /locus_tag="PC541_003684"
     CDS             complement(603..1022)
                     /locus_tag="PC541_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841099.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            complement(1042..1767)
                     /locus_tag="PC541_003685"
     CDS             complement(1042..1767)
                     /locus_tag="PC541_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841100.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            complement(1751..2113)
                     /locus_tag="PC541_003686"
     CDS             complement(1751..2113)
                     /locus_tag="PC541_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841101.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            2680..3072
                     /locus_tag="PC541_003687"
     CDS             2680..3072
                     /locus_tag="PC541_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2841102.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
BASE COUNT         1156 a          526 c          273 g         1408 t
ORIGIN      
        1 tattgatatt atttagttat atagttctca ataattatat tttatatgac ttattgatgt
       61 cacttctttc tcttaaattt atgtatttct atatactttc actaaaattc catactttta
      121 ttttgaaatc agtctcatat ttttgactac gttttgataa tttcaattta atattaacat
      181 attgaccatt attagtaatt gatacataca tctcatctac ataatctcca atttcataac
      241 cacctgcttg actactttcg ccatttaaag aatttacaaa taacttattt cgtctttcat
      301 tcaatataat ttctttatta cttatgcctt cttcattatt attttcatct aaatatctac
      361 attttataga tgttgtcttt ttaaatgttt cttcataatt agatacattc attattccca
      421 tagaattgct aataattcct tccatagatt ttatgatttc aagactttgt tgttgtagtt
      481 ctatcttatc ttctactttt aactttaagt ttattgtaga caaaaatata gaatataaaa
      541 ctagtataat tatcaccatt gaagacaatg atacgacact ttccaaaagc aagtaaccat
      601 atttatgttt catattttcc ctctttcaga agtactcttc cacttacagg tgttattgtt
      661 atagtttttc ttaatctatt attatatatc tctattgtag acgccttttg aggcgaccca
      721 tcagatttaa actttattat ttcaatactc ctagctattt ttgcattttt aggtagcatt
      781 attgatttaa tgtttttacc atcttttctc aatacataac cttcgtgttt accttgattt
      841 atataatata tatatgtatt gtagttgcta aacatattaa ttcgcctaac ataccttata
      901 tctgaacaaa gttgacgtgc aaataaattt attttatgat tttcaagatt atcttttgga
      961 aaagataatg taactatcaa gagtattata ctgatagata ctattaattc tattacagtc
     1021 acattccttc ttttttcaaa gttatacacc cctatactta tatacctgta ctaggttttt
     1081 ttcatcaaaa ttcttcttta tattttcaat tatgttttgt ttaagttctt cttcatattc
     1141 ttcatcttct tcatcttctt ctgtaaatcc accaacctct tgattcctta tattttcact
     1201 ttcattttta aattgattta gaatttcatt attttcagat gctttataat tattgaatgt
     1261 tttgtcatca attttattgt tattagattt atccatataa tcttcaatgt caattcctat
     1321 ccatggattt ttgattttca ctcttacttt tattgctttt aaaaattctt tttctctaac
     1381 attactttct atatcaaatt caaaaaaatc tcctctgtca taaatagtat tattttctac
     1441 ttttaaagaa agttcttcta aactactata agatgtatct tttatttttt ttatacattc
     1501 aatcatatta tttcctaaga aatattctct aaattcgtct tcattattag atacatcaat
     1561 agcttcctca acacaagaaa gtacattact tactgatatt tctattccac ttaaagaaat
     1621 ttcttttagt tgaatgtcct tgtatcctaa ctcaaacata ttattatgtg tataaattaa
     1681 acctatacac ataatgcaca cagtacttat aatagaaaat attatcaatg ttattattaa
     1741 tatagagcct ttattttcta gtaacatagc tattaaacct cagtttttta aattcatttt
     1801 ttatctctat atttatttca tagcattgat aataatctaa aatttgctct attttactgt
     1861 taatttcata tccatttatg taattttttt ctatcttatc ttcaattaac tcatacttac
     1921 tattttgtat tctatacttc tcatcttcta tatatttttt tgctgtactt aacatttctc
     1981 tgtcagatat atttctactg ataaaactat aacaattata caatgatgta gaaactaaat
     2041 aaacagccac atataatata gcaatactta ttatacattc aattgatata aaaccactct
     2101 ttcttatttt caataataaa aacaacccct aaataatatt ttatatataa acatttaact
     2161 ataagttttt tcataaatat cttttgtaag ttttttcatt gtaatcaaaa tttcttccca
     2221 tacatatata aattaacaca ccaccttcac ccttaacagt attaactatg actgatactt
     2281 ttgcttgtaa tcaactttat acaaacaatt aattacattt acttttttaa gtattgtaaa
     2341 attacacctc ctatattcat atttttatag actctaatgc tttgcattaa tcttttactt
     2401 caacaccaaa acatatataa atactatttc cttttatatt tattgacacc tcaattccaa
     2461 tttatacttt ataaatcaat aatttcttcg ccttatttca tatatataca acaaaaactt
     2521 aaatttaaat tcccactctt tatgtcatat tccattcaaa agtttgcaaa tatatgtttt
     2581 aaattctaaa aatatgcata ttattttatt agataacagt caacttggat aagtctcctt
     2641 catataattt aaagtgaccg ttgaaaaggg ggtgacctat tggcagctct taaatctttt
     2701 gaaaaaccct taacccctga agaagagatt gagtatttaa ctaaatttaa aatagaaaac
     2761 gataaaagtg caaaagatac attaattgaa agaaacatga gattagtagc ttatatagca
     2821 aagaaatata ataattcaac ggaagatcag gatgatttaa tatccatagg tacaatagga
     2881 ttaattaaag ctatagaaac ttataacata gataaaggaa ctcgattagc aacatatgct
     2941 tctagatgta ttgaaaatga gattttaatg aatattagat ctaacaaaaa aaataaaact
     3001 caagtatctc tacaagaccc tatcggaaca gataaagaag gtaatgaaat aaacactact
     3061 ctttatatgt aattttctac tattatatat ttatttactc tataaaataa tccccattca
     3121 aaacactttt cttaaatcac tcaaactaaa atacatataa gattatcatt agtcagtaag
     3181 tatatatttt tctaatttct aaaaatacat ccaaatcatc aaacgttttt agcaattact
     3241 ttattattat acagtgttaa agtaaagttc ctaaataaat tgttattcat atagcaaata
     3301 aatacttggt atcaattttt taatatctat ttaaatagta ttttttattt tgtcacttta
     3361 tag
//
LOCUS       DAMJZE010000269         3262 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJZE010000269 DAMJZE010000000
VERSION     DAMJZE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.274"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..619
                     /locus_tag="PC541_003688"
     CDS             <1..619
                     /locus_tag="PC541_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841103.1"
                     /translation="GEQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYD
                     IEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNL
                     VYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFK
                     GIIDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            985..2166
                     /locus_tag="PC541_003689"
     CDS             985..2166
                     /locus_tag="PC541_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2841104.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(2308..3225)
                     /locus_tag="PC541_003690"
     CDS             complement(2308..3225)
                     /locus_tag="PC541_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841105.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         1273 a          372 c          531 g         1086 t
ORIGIN      
        1 tggagaacaa caaaatatca atgaaattaa attttctatt ggaagtaaat tatacttaga
       61 aatagtaaat aataaaagta aaaatggata tattgaagga catgatagtt actctaactt
      121 tattatatat gatatagaag ataacacttt taatataagt aataaattta tggataatct
      181 aaacaaagaa aagaatgcaa atccaaagga gattggctta ttaagtaaaa ctaagctaga
      241 ttatatggta aaaaacaata aattgaatct tatagttacg gatatgagta attccataac
      301 taatcttgtt tatgatatta attctaatga gattaaatta gaagattatg agatacttga
      361 tttaaaagta gattataatg aagcatcatc tggagttagg aaagttaagc tactggatga
      421 taagatatat tctatttcag gtattaagaa cagtatatca ggagaattaa aaggatttaa
      481 agggattatt gatgtagtcg gaactaagcc agtaaaattt caagtatttg atatgaaact
      541 taaaaagaat atttatgaag ctgagctaat aaatggagat ataagtttac aagaaaagtt
      601 attttttgtt gcaaattaag tgttagaatt aattttagaa ctttaaactt ttatatgtat
      661 cacagataag tataagtaag ggagtgtctc aaaataaact ttttagctca tgagacactt
      721 tttttatatg aaatcaaata tattttcatt gtttagattt attttgataa tgaaaaaaag
      781 attatcttta ttttaagaaa gacatttatt tataattata tacaaaaata ttttttaatg
      841 tttttgtgta taattataac aatttaattt atattaatgc acttaaattt gaaatagaat
      901 gcttatagtg gtaaaattaa ttattagaat attagaaata ataaataaat aatacattgt
      961 acttaaaagg gggagaaatt taagatgaac aaaaaatata ttaaagcagg gaatttaatt
     1021 gatggaaccg gaaatagtat tatgcaaaat aagggtataa ttattgatgg agatactata
     1081 gttgaaattg aagatataag agatgattta aatggatatg atgtgtacga ttacttagat
     1141 aaaactgtta tgccaggcat tatgaattgt catgttcact tgacaatgga gccagttgga
     1201 aatccaaaga tatattatga taatgtatca gatgtagagc ttgtagtaaa tacgataaaa
     1261 caacttgatg cttatttgga ttcaggagta acatatatta gaagcttagg ttgtcccaaa
     1321 tatgtggatg tacaacttaa aaatcttata gacaaagggt ctatagatgg tcctggtata
     1381 gttacttcag gtccagttat atgtatgaca ggagggcatg gatattattt tggtattgaa
     1441 agtgatggag tagatgaatg tagaaaatca gccagaacag ttttaaaaaa tggagtagat
     1501 tgtgtaaaga taatggcaac tggaggtgta acaactgatg gagttgagcc agggtcacca
     1561 caattaactt tggaggaaat gaaggctgct gtagatgaag ctataaaagc aggtaaaata
     1621 actgccacac atgcacaagg aagaactggt ataaaaaatg cagtgcttgc aggaattact
     1681 tctatagaac atggtgttta tttagatgat gaaataattg atttgatgct tgaaagagga
     1741 acttacttag ttccaactgt ggctgcacca tattttattc taaatgcagg aaaagaaagt
     1801 ggaataactg aagctactct tagaaagtgt gagattgttg ccattccaca caaagagtca
     1861 ttcctaaaag catataaaaa aggaattaaa attgcagttg gttctgatgc aggaacttct
     1921 tataatgaac atggaaaaac atattatgaa atgaaactaa tgtcagatta tggtatggat
     1981 aatatggata taatagttgc tgctacaaaa acagcatctg aacttttaag aattgataaa
     2041 aattatggaa ctctagaaaa aggaaagaaa gcagatgtta tagttgtaaa tggaaatcca
     2101 gtagagaata tagacgtact tgcagatgtg ttagcagtat ttaaacttgg taaaaaagtt
     2161 agataaatag tataaaaata aaaaagagtg tctaaagaaa tttttaaata attatagaca
     2221 ctctttttta ttagtaataa ttaaatcttt tatactttag aaaatagatt ctattttata
     2281 tttagatata actctttaaa atcattctta tgcaatataa tctatagaat ctacacatac
     2341 aaagtatatt cttgaatcat tagaatttcc aagtactata acatcatttt tcttacctag
     2401 aacttcaaca ccagttagaa ccaaactacc agctactaca gtaacttgat tatttatatt
     2461 tgacatataa agattatcta ggataccttt attacaacag caatcatcac aatcgcagtt
     2521 gcaattacat tctttgtaat gttttttatc aagatagtaa cctaaagatg ctatgaaatc
     2581 ttcataatct gcaggtgtaa tatcaaatgc aacagcttta agattacaca atgatgctaa
     2641 atctacgttt gctaatggag tagatgcagc aagtataaaa tcaattatag cagcaagtat
     2701 tgcatctaat atagccccaa ggtctatcgt aggaggaata actgcatcaa taagtgcaat
     2761 taattcagct actcctggta tagttcctat agtattaatt aatccctcaa gagttaaagg
     2821 gactggaata ggataagata ctctaccagc tatatctatt aaatcacagt tacaagcaga
     2881 aaatctttca aaagtaccat caagtccaga taaattatct tttggaggag ctgaaagatc
     2941 tatacctacc aaattagcac ctactataaa gaaatctgag ataaaagcaa attgattaaa
     3001 gtttacaaaa ggtcttagag catcatatct tagaagttct aatgcttctc tcatactttt
     3061 tttacaacaa cattgtttta ttttaggttc tttatattta tttggataat tatagtatct
     3121 atcagctact tgataatttt catagtatct ttcatttgta tttggataat cttcctcata
     3181 ttcttgtgta aatctaaagt cctctctaca tttattattt tccactaaat acactccttt
     3241 tatattttaa tttaaatgta ta
//
LOCUS       DAMJZE010000270         3211 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJZE010000270 DAMJZE010000000
VERSION     DAMJZE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.275"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..450
                     /locus_tag="PC541_003691"
     CDS             <1..450
                     /locus_tag="PC541_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2841106.1"
                     /translation="EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKF
                     KKAIRELLWFVCEYLKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIISDET
                     IVSNHPWVEDVNDELDRLKKQKEESIKEYEDAFPTKKKTEEGEPLDE"
     gene            443..1891
                     /locus_tag="PC541_003692"
     CDS             443..1891
                     /locus_tag="PC541_003692"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2841107.1"
                     /translation="MNNINYWIKTFNQLEEDIHNKGDNFNKELEKQYDIILYNILREI
                     NNWYMKFAKDNKISMQEAEKLLNSKELTELKLSLEEYIKYGEENVISQKWMKELESAV
                     KRVRISRLEALKLKIRHQVEVLYSKEYEDVNTLITNTYKDSYYHTAFEAQKGIGIGLM
                     IAMLSMNNINKIVSSPWTTDGITFGNRIWNKHRPRLISELNKGLKQTLINGVSPENLT
                     NKINKNFNTSKEEAKLLVKSELAFFSSLSQRDCFNSLGVEKYEIVSASDSRVCEKRCS
                     PLDGKVIEMKYYEIGITAPPFHPRCRCVVVPYFDGEESYRAAREEDSETYYVPASIKY
                     NEWYSKYVKDSFSSGAISRKRLKDLEIYLREEKHANLYYEEIRKRTSDIKAIAKNTGY
                     SEKVIQDIKSHVFMNKYNLYGGYKSFDPSYDMAISWQRLIEGKDIRKCDIILLKHERL
                     EKFLMDRYNYDYKKAHGLVVRKYDYEKALREG"
     gene            1894..2136
                     /locus_tag="PC541_003693"
     CDS             1894..2136
                     /locus_tag="PC541_003693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841108.1"
                     /translation="MVLIKKNYIKDDKVSYFYQPEEDGEFGEVILYTKDKSFDIVKIA
                     ERDSEGNHFYMSHVYCMIRKFIEENNYPDEKTSYWY"
     gene            2207..2401
                     /locus_tag="PC541_003694"
     CDS             2207..2401
                     /locus_tag="PC541_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841109.1"
                     /translation="MCILTQVIVVVCVVQIVINCIANLNVGILCNKLKKENKANIDKV
                     SDEILKRVREELNKSLDKSL"
     gene            2443..2673
                     /locus_tag="PC541_003695"
     CDS             2443..2673
                     /locus_tag="PC541_003695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841110.1"
                     /translation="MKEYIIWFKSGNCVSGITDEYVADKLMKDFMGADSDCRNLKGYL
                     DEDGTTIIDLSQIEAMSINNCNENNNIGFSKS"
     gene            2718..>3211
                     /locus_tag="PC541_003696"
     CDS             2718..>3211
                     /locus_tag="PC541_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2841111.1"
                     /translation="MDWLKELLEGIKIEDNKIDVVSLQKSIEKKIKETTVTQEEYTNI
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPG"
BASE COUNT         1378 a          287 c          594 g          952 t
ORIGIN      
        1 gaaaagaata taattatctt tggtcaagga gttaatccag aatctcaaaa tacaggtgac
       61 aaatcgggtg tggcacttaa atttttatat tcactacttg atttaaaatg ttctaagact
      121 gaaaagaagt ttaaaaaagc aattagagag cttttatggt ttgtgtgtga gtatttaaag
      181 ataagtggta gtaagagcta tgattataaa acagttcaaa tcacttttaa tcactctatg
      241 ataataaatg aagctgaaaa gatagatatg gcagctaaat caactggaat tatatcagat
      301 gaaactattg ttagtaatca cccatgggtt gaagatgtta atgacgaact tgatagactt
      361 aaaaaacaga aagaagaaag tattaaagaa tatgaagatg cttttccaac taaaaagaaa
      421 actgaagaag gtgagccttt agatgaataa tataaattac tggataaaga catttaatca
      481 gttagaagaa gatattcata acaaaggtga taactttaac aaggaactag aaaaacaata
      541 tgacataata ctttacaata tccttagaga aataaataat tggtatatga aatttgctaa
      601 ggataataaa ataagcatgc aagaagctga aaagttatta aattcaaagg aattaactga
      661 acttaaatta tcattagaag aatatataaa atatggagaa gaaaatgtaa ttagtcaaaa
      721 gtggatgaaa gaactagaaa gtgcagtcaa aagagttcgt ataagtagac tagaagcatt
      781 aaaattaaag ataaggcatc aagttgaggt attatactca aaagaatatg aggatgtaaa
      841 tacacttata acaaatacct ataaagatag ttactatcat actgcttttg aagctcaaaa
      901 aggtattgga attggattaa tgatagccat gcttagcatg aataatatta acaaaatagt
      961 tagtagtcct tggactactg atggaataac atttggtaat agaatttgga ataagcatag
     1021 accaaggtta ataagcgaac ttaacaaagg attaaaacaa actttaatta atggagtatc
     1081 tcctgaaaac ttaacaaata aaattaataa aaattttaat acaagtaaag aagaagctaa
     1141 gttattagta aaatctgagc ttgccttttt tagttcatta agtcaaaggg attgttttaa
     1201 tagtctaggt gttgagaaat atgaaatagt aagtgcatca gatagtaggg tttgtgagaa
     1261 aaggtgtagc cctcttgatg gtaaagtaat tgagatgaaa tattatgaaa taggaattac
     1321 agctccacca tttcatccta gatgtagatg tgtagtagta ccatattttg atggtgagga
     1381 aagttatagg gcagcaagag aagaagatag tgagacttat tatgttccag catcaataaa
     1441 gtataatgag tggtattcga agtatgtaaa agatagtttc tcaagtggag caataagcag
     1501 aaaaagacta aaggacctag aaatatatct aagagaagaa aaacatgcta atttatatta
     1561 tgaagaaata agaaaaagaa ccagtgatat taaagctata gctaaaaata caggctatag
     1621 tgaaaaagta atacaagata taaaaagcca tgtatttatg aataaatata atttatatgg
     1681 cggatataaa agttttgacc ctagttatga tatggctatc tcttggcaaa gattaattga
     1741 gggaaaagat ataagaaaat gtgatataat attattaaaa catgagagat tagaaaagtt
     1801 tcttatggat agatataatt atgattataa gaaagctcat ggtttagttg tgagaaaata
     1861 tgattatgaa aaagctttga gggagggata aacatggtac ttataaagaa aaattatata
     1921 aaagatgata aagtatctta tttttatcaa ccagaagaag atggagagtt tggagaagtt
     1981 atactttata ctaaagataa atcttttgat atagtaaaaa tagcagaaag agacagtgaa
     2041 gggaatcatt tttatatgtc tcatgtttac tgtatgatac gtaaatttat agaagaaaat
     2101 aattatccag atgagaaaac atcttattgg tattagaaag cacttgctaa ataatatatt
     2161 agtaggtgct tttattatgt aaaaatttat tgagagggtg attttaatgt gtatattaac
     2221 tcaagttata gttgtagttt gtgtagtaca aatcgttatt aattgtattg ctaatcttaa
     2281 tgtaggtatt ctttgtaata aattaaaaaa agaaaataaa gctaatatag ataaagtttc
     2341 tgatgaaatt ttaaagagag taagagaaga attaaataaa tcattagata aaagtctata
     2401 agaagttttt tattaaataa aaatttaagg aggcttattt tattgaaaga gtatataatt
     2461 tggtttaaga gtggaaattg tgtatctgga ataacagacg aatatgttgc tgataagcta
     2521 atgaaagatt ttatgggagc tgactcagat tgtaggaact tgaaaggata tttagatgaa
     2581 gatggaacaa caataataga tttatcgcaa atagaagcta tgtcaataaa taattgtaat
     2641 gagaataata atattggttt tagtaagtcc tagatagggc ttttttatta tgtaaaaaaa
     2701 gaaaggagac atttaaaatg gattggttaa aagaattgct agaaggaata aaaatagaag
     2761 ataacaagat tgatgtagtt tctcttcaaa aatctataga aaagaaaata aaagagacta
     2821 cagttactca agaagagtat acaaatattg aaacacaact taatacagct aatgaaacta
     2881 ttaaaaagtt tgaaggaggt atgacaaaag aagatgtaga gaatctaaaa acaacttatg
     2941 aaactgataa gaaaactttg gaagaaacct acaaaaaaga aattgaagaa aaagatttta
     3001 attactggtt aggtgatgct tttaaatctg ttaaatgtag agatgaaata gcattaaaag
     3061 ctcatttaga tatagaagca ctaagaaata gtaaagacag acaaaaagct tttgaagagc
     3121 aaataaatcc tttgaaacag gataaagatt atttgtttaa tgcaacacta gaaggtgaag
     3181 aacctaaaat agatactata acaccagggc a
//
LOCUS       DAMJZE010000271         3129 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJZE010000271 DAMJZE010000000
VERSION     DAMJZE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.276"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            81..623
                     /locus_tag="PC541_003697"
     CDS             81..623
                     /locus_tag="PC541_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2841112.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            637..3129
                     /locus_tag="PC541_003698"
     CDS             637..3129
                     /locus_tag="PC541_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2841113.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
BASE COUNT         1273 a          400 c          494 g          962 t
ORIGIN      
        1 tagcttacat attgattaat aaattttaaa ggagtataat aaacagtgtt cataataatt
       61 ttaataaaag aggtggtaat atgccaaaga ttttagaaaa tgtaaaagaa gatatattaa
      121 aagtgtctcg ggatatgatt ttagaagaag attactcgaa tattagcata aggaaaatag
      181 ctcaaaggtg tggtataagt gcaggtacag tgtataatta ctttaattca aaacaagaaa
      241 taatagaata tatcacaaaa tctgaatggg atttattgat aaggcgaatt gaatactcca
      301 ataaaaacac tgaagattac ataaaaaaac taaacattat ttttactgaa ataagaaatt
      361 ttataaacaa agttcataat atccaataca atgacttttt aaacactttt gaaacagaga
      421 gattttttga aatgaaaaaa cacaaagacg actttcatga acagctttca aataaagtat
      481 atgaagcttt agaaaaagaa agttttttta ataatgacaa attagtatgc aatatcataa
      541 taaaaatgtt tttttcatat tcaacatatc ttcatataga atttgaagat ttaaaaccct
      601 atattgaaaa attaatacat taaggtggtg tttaccttga atcataatac tagtattaag
      661 aacaaggaca gtaatccaaa aatattttta gtacttacgc tttatctatt aggtatattt
      721 atgggagcta ttgatactgg aatagtatca ccagcaagaa cgattataca aaatagcctt
      781 ggagtaaatg aaaaaacggg aatttggatg attaccatat acacattaac atacgcttct
      841 gtaattccta tctctggaaa attagcagat aaaataggaa gaaaatatgt gtatctagtt
      901 agtatatttc tatttggtag tggttcatta atatgtggat tatcatcact attttccaat
      961 ttctatattt tattaattgg tcgtgttatt caagcaattg gtggtggtgg aatcatgcca
     1021 attgctacag cagaatttgg aactaccttc ccagaaaata aaagaggcat ggctttaggt
     1081 ttagttgggg caacatatgg tattgccaat attttaggtt caagcattgg tagcacaatt
     1141 ctaagtatct ttggaactca aaattggaag tggctatttt ttgtaaatct tccaatttgt
     1201 ttaatcatac ttattggtgg agttttctgt attaaaaaca ataagtctga atctaaagaa
     1261 aaaatagata agcttggtac attgatgttg atatgtataa tcgtatcact attatatggt
     1321 cttatgaata tagacttttt taattttaaa aattctatac aagatatatc agtttatcca
     1381 tacttattga catttattgt acttatacct attttcatat ttattgaaaa tagagctaaa
     1441 gaccctattt taagctttga atatttttta aatccgagaa tattgatagt acttatttta
     1501 tctttaattg ttggtattgg aatgatgggt atggtattcg ttccacagta tgctgaaaat
     1561 gcattaaaga taaatgctgg ttctggggga tattttgtag ctatattggg attatttgca
     1621 ggtattgctg ctcctttaag cggaagactt attgacaagt ttggggcaaa aaaaatacta
     1681 ttactaggat tttctgtatc tatgataggc tctctttacc tagttttaat tgctctaaaa
     1741 accaatactg tatttagtgt atgtgtaagt ttaatattta tgggattagg aatgggattt
     1801 actatgggaa ctcctctaaa ctatatgatg ttatcaaata caagattaga agaatctaat
     1861 tcagcacttg caacattatc cttgatacgc tcaattggta cttcaatttc tccagctata
     1921 atgattggat ttatagcaca tgcaggatta tctgtgcaag ataatattat ggacttagtt
     1981 ggaaagccct caactcctaa aatagttcaa cttgaagaac ttgaaaacat gatagatgac
     2041 ttaaaatcag accctgaaat ggcaaaaaat ttaaaaaatg tctctatccc aaatatgaat
     2101 gatacttcaa atataaatat ggacatgaca ggtggcaaac ttccaaaatc cttacttaat
     2161 aaagttcagt cagcagatac aaccaacatt accaatataa caaaagaaat atcaacaaca
     2221 atgtttgatg aaaaagttcc atctataatt actaaaatac agaaaaatgt acaaaaagga
     2281 atagatggaa cacaaagtgg aataaacggt attgaagatg gtgaaaataa actaaatagt
     2341 ggtattaaag gagttcaaac tggtattgac aatatggtaa aagctagagc tggtattaat
     2401 caaggaatag atggtgtaaa aaaaggtatt gctggtgttg ataaaggtat aaaaggtatg
     2461 gaacaaggtc ttgaaaaaca agatgaggcc atcaaggaac ttacaactgc ttataataaa
     2521 atacctacta cagaaaaaac tcctactgat aacgaaaata actcaaatga acaaaaccag
     2581 agtaatacca gtaattctga atcagacgac actaagaatg attctacaga aaataagaat
     2641 aacaattcca gcgagaataa accaaatgta aatgaacaaa aagaaagttt aaatgctcaa
     2701 atacagcaac taaaaaagac aagagatgac ttaaatacaa agttacaaaa aaataaaact
     2761 caaaagaacg aattaagtaa aaaactaaga acaatggaat ctcaaaagaa agagctacaa
     2821 agcaagttaa ataattctat aagccaaaag aaagacatgg aaaaaagcct tgatacaatg
     2881 caacagcaaa aaaaatcact ccaagcagtt ttagcaaaga cacaagaggt aaaaactgaa
     2941 ataccaaagg cttttgataa atcaaaattg gattatataa attccataga aaataataga
     3001 acaaaaatag aaaacacact acaatctact ctaaactcag gatttaaaca gatgtatata
     3061 acagtattct gtgtaaatct actagcattt ataatattat tgttctataa agaaaataaa
     3121 actagataa
//
LOCUS       DAMJZE010000272         3105 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJZE010000272 DAMJZE010000000
VERSION     DAMJZE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.277"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     misc_binding    82..344
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            420..3086
                     /locus_tag="PC541_003699"
     CDS             420..3086
                     /locus_tag="PC541_003699"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2841114.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1256 a          347 c          610 g          892 t
ORIGIN      
        1 acataattaa gaaaattcag aaaaaataaa ataaaaataa ttgacaaaga gaataatatt
       61 tgtatataat aatatgtaat attaaagact gagaagaaga ggagtaagta aatactacta
      121 aaagagagga aaattcactg gctgtaagat tttctaagta taagatttac tgaatgtagc
      181 tttggagttt ctaaactgaa ctataagtaa gtttagacgg ttagaaccgt taaattcttt
      241 gagagtcaaa gttgataaaa taacattaac caataattta atgatatgtt aatgtatttt
      301 aagctttgga attaaggtgg taacgcgagc ttttcgtcct ttttaaagag gatgaagagc
      361 tcttttttat ttctaaagat atttaaaaaa tttatttata aaatacagga ggcaggacaa
      421 tggaaaatac gaatttatca aaaacttata acccaaaaga ttttgaggct aggctatata
      481 aaaaatggat ggatgaaggg tattttaaat ctaaaccaaa tccagataaa aaaccattta
      541 caataatgat gccaccacca aatataacag ggcaattgca tatgggacac gctcttgacc
      601 atacacttca agatatatta atcagatgga aacgtatgga tggatatgaa gctttttggc
      661 ttccaggaac tgaccatgct tctatagcaa cagaagttaa agttgttgaa agaataaaaa
      721 aacaagaagg caagacaaag tatgaaatag gtagagaaga atttttaaag agagcctggg
      781 aatggaaaga tgaatttgga ggaaaaatat ctaatcagct taaacaacta ggtgattctt
      841 gtgattggga taaagaaaga tttactatgg atgaagggtg taatgaagca gttatagaat
      901 tttttgtaag cctatatgaa aaaggtcaca tatacagagg aaatagaata ataaactggt
      961 gcccagattg caaaactact ttatcagatg cagaagttga acatgaagaa cacgacggaa
     1021 acttttatca tataaagtac ccactaaaag atagtgaaga ctttttagaa atagctacaa
     1081 ctagacctga gacaatgata ggagatactg gtatagcagt aaatccagaa gatgatagat
     1141 ataaacattt aataggaaaa acagcaatac ttccattagt aggaagagaa cttccaatag
     1201 ttgcagatag ttatgttgat ttagagtttg gaactggtgc agttaagatg actccagcac
     1261 atgaccctaa tgattttgaa gtaggattaa gacataatct tgagcagtta aatactatga
     1321 atgaagatgg aactatgaat gaagtttgtg gaaaatatga aggtatggat agatttgagt
     1381 gtagaaaagc tatcgtagca gacttaaaag aacaaggtta cctaattaag ataaaagagc
     1441 ataatcataa tgtaggaact tgttatagat gccatacagt agttgagcct agattatcag
     1501 agcaatggtt tgttaagatg gaagaacttg caaaaccagc tatagatatt cttaaaaaag
     1561 gagaattaga atttgttcca gataaatttg ataaaactta tttacaatgg ctagaaaata
     1621 taagagattg gtgtatatca agacaattat ggtggggtca tcaaatacca gcttattatt
     1681 gtcaagagtg tggagaaata gtagttgcta gaaaaatgcc agataagtgt cctaaatgtg
     1741 gaagcactca ttttaaacaa gatgaagatg ccttagatac ttggttcagt tctgcattat
     1801 ggcctttctc tactctagga tggccaaata agactgaagc tttagattat tattatccaa
     1861 caagtgtact tgttacagga tacgatataa tattcttctg ggtagttaga atggcatttg
     1921 caggtatgtt ctgtatgaat gaaaagccat ttgaccatgt tttggttcat ggattagtaa
     1981 gagattctca aggtagaaaa atgagtaaat ctttaggaaa tggtatcgac ccattggaga
     2041 taatcgagca atatggagct gatgccctaa gatttacatt aacaactgga aattccccag
     2101 gaaatgatat gagattctat atggaaagag tagaatttgc tagaaacttt gcaaataagt
     2161 tgtggaatgc atcaagattt gtatttatga atattgatga agatataatt aaaaacatga
     2221 ctagagaaag tgttaaagag gacttaactc tagctgataa atggataata tcaagagcaa
     2281 ataatattgt aaaagaagca actaataaca tggataagtt tgatttagga atagcacttc
     2341 aaaagatata cgattttact tggtcagaat actgtgactg gtatattgaa atggttaaac
     2401 ctagattata tggagaagat gcaaatgcta aatctgcagc attatataca ttaacttatg
     2461 ttcttgaaaa aatattaaaa ttattacatc catacatgcc atttataaca gaggaaatat
     2521 atactcatct tcctactgtt gaaggatgta taatagtatc tgaatggcca aaatataatg
     2581 aagaagataa catggctgaa gaagaagata tgatgaactt attgatggaa ggtataagaa
     2641 gtataagaaa tgtgagagca gaaatgaatg taccaccttc taagaaagct aaattgataa
     2701 taatcccaag tgaagaaaaa atagaagcta ttgagcttgg taaagactat tttataactt
     2761 tagcatctgc ttcaaatgta gagatagcaa aagataaatc taatgttcca gaagatgcag
     2821 ttggagtagt tattgatggt gttgaaatat ttatacctct taatgaatta gttgattttg
     2881 aaaaagaaat tgagagatta tctaaagaaa agaaaaagtt agaaggcgaa ataaagagag
     2941 ttaatggaaa gcttgcaaat caaggtttct tagcaaaagc tcctgaaagt ttaattgaag
     3001 aagaaaaagc aaaaaaagaa aaatttgaag agatgattaa atctgttgag gaaagattaa
     3061 caaatttaga atctaaaata aaataaatat aagaattagt aaaaa
//
LOCUS       DAMJZE010000273         3051 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJZE010000273 DAMJZE010000000
VERSION     DAMJZE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.278"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            156..950
                     /locus_tag="PC541_003700"
     CDS             156..950
                     /locus_tag="PC541_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2841115.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            951..3011
                     /locus_tag="PC541_003701"
     CDS             951..3011
                     /locus_tag="PC541_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2841116.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1169 a          316 c          502 g         1064 t
ORIGIN      
        1 gatattataa acgaataaaa aataataatt tgtcagataa acttgtaaat ttttatgaat
       61 aatccattct aaaacaaaaa actgtgtata aaaaaggttt atatgttata tttatataaa
      121 tataacatat gaacgagaaa ggagtggttt gataaatgat tgaggtagat aaattgtgta
      181 agtcttttac aagggttgta aaagatgata agaataaaag ttcaataaga aagctaaaga
      241 aagtaaaaac taaaaaagaa gaattttttg ctgtaaataa tgtttcattt aaagttatgg
      301 aaggtgaaat tgttggaata ttaggtccaa atggtgcagg aaagactaca ttacttagaa
      361 tgttaggtgg catattaaca ccaacatcag gaagtataaa tatttctgga tatgattatt
      421 caatagatag aaattcagca aaaaaagaaa taggttatct ctctggaaat acaaaactat
      481 atggaaggct ttctccgaga gaacttctaa caacttttgc aagtctatat gagatgtcaa
      541 aagaagatat agaagaatca attgaaaata ttgtaaaaat aatggatatg agtgaattta
      601 tagataatag aattgaaaat ctatcaactg gtcagactca aagaacatct atagctcgtt
      661 gtttaataca ttctccaaaa gtatatattt ttgatgagcc aacactaggt ctggatgttt
      721 taagtagtgc atctattata gattttatga agaatgaaaa actaagggga aaaactgttc
      781 tttactcaac tcattatatg gaagaagcag aaactttatg tgacaagatt tttatgattc
      841 acaatgggaa aataattgca tcaggaacac cacagagttt aaaagaggaa tctggagtaa
      901 ataatttgag agatgtattc ataaaacttg ccagaaagga ggacatttaa atgagaagta
      961 agattgtaaa atacatattt aaaaaagaaa tgttagacat attgagagat aaaaagactt
     1021 tatttatgat gattgttgtg ccacttcttt tatatccttt acttatgtta ttaatgattc
     1081 aagtaatgaa tatgtctgca agtagtatga atagtaagga cattaatata gcttttaata
     1141 ataaacctaa taaggtgtta gtatccatga ttgaagatga taatttagaa aaagatacta
     1201 aaaatacttc aaatgataaa ggcaaaataa gagtgttaga tgtagataac tataaaaagt
     1261 cattagaaga aaataaaatt gatgcttata taaagatgga agaaaaaaaa tcatctataa
     1321 attatcaaat atatataaat tcttctcaag aaaattcttc taatgcacta aaaagaattg
     1381 aaactgtttt ggataaatat aaaagattta ccatagaaaa aactttatct gaaaaaggat
     1441 tagatgtaca agctacttta gaaccaataa catatagcac tgtagatgtt gcaaagacgg
     1501 aagaagtggc aggatacttt ttaggtcaga tacttccatt tatattaata ataggagttt
     1561 tactaggttc aatatatcca gcaatagatg ttatggctgg agaaaaggaa agaggtacat
     1621 tagaaacatt atttacactg ccaatatcaa acttagaact ggttatggga aaatatatgg
     1681 cagtttctct tagtgcaata gtaacagcaa ttttaaacat tttatctatg ggactgacta
     1741 tggcatttat tttagttagt ggaggtatta gtagccaatt tggctttgga aactttaatt
     1801 atggagactt agtcttacca attataacta ctttaatttg catatgtctt ttttcaatgg
     1861 tagtttcagc aataagtatg tgtgtatgtt cattagcaaa aagttttaaa gatgctcaaa
     1921 actacataac acctcttatg ttaattatta tgataccatc atatgtttct atgataccaa
     1981 atatagtatt agatagagtg acagctgtaa tacctgtagt aaatatatct ttacttataa
     2041 aaagtgtatt atcttttaaa agcgatttaa atttaatagc cttagtttta gcatcaaatg
     2101 tagcctttgt tatattggca atcatactac tgtctaagat gttcaattct gaggaaatac
     2161 tttttggtaa tagtaagagt ttttctttct tagagaaaag aagtaatata aaaaaagggt
     2221 ctataccaac tgtaagtgat ggtgttattt tatatgcagt aggattagtt ctattgattt
     2281 atgttggttc tttagttcaa attaaatttg gtatggcagg tattataatg acacaattca
     2341 tgataatttc tttaccatta ttatttgcat actatattaa agctgatttt aaagaggtat
     2401 ttagcttgac agttcctaaa ttaaagcatg tgtttgggag cattgttctt tggattggtg
     2461 gatacatact tattatgatt ataacacagc taattttatt cttatttcct caaaatatgg
     2521 aagttgctga aagtttaaat aaggctcttt ttatcaaaga tagtgtttta ctgaatttac
     2581 tagcaatagc tttattacca gctatatgtg aagaaatgtt ttttagggga tttatatttt
     2641 cttcatttag taaatcaaaa gataaaaata agtctatcaa actcgctata atatgtagtg
     2701 gggtactatt tggtattatg catatggatt ttattagaat aattccaaca tcaatattag
     2761 gtattatatt tgcatacagt gtgtataaat ctggttcaat atttgtatca atgttattgc
     2821 attttttaaa taatagtgta gcagttgtgt taaatcatta tacaactgga aatataacca
     2881 acttatataa atttgttgaa gttgattttt cgaacttaaa tgtgcttaaa tttgccttaa
     2941 ttcttctaat aagtcttata ttaattatgt taggattaag agttttagat agaaagtctt
     3001 taagaaatta agtataaagt tattatatag attaaaatat taataagcac t
//
LOCUS       DAMJZE010000274         3025 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJZE010000274 DAMJZE010000000
VERSION     DAMJZE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.279"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(40..648)
                     /locus_tag="PC541_003702"
     CDS             complement(40..648)
                     /locus_tag="PC541_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2841117.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(757..>3025)
                     /locus_tag="PC541_003703"
     CDS             complement(757..>3025)
                     /locus_tag="PC541_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2841118.1"
                     /translation="LLYLLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDS
                     DRLIGVIYLLLCNHTYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKD
                     MIIQRTLKNYIDQRKELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYK
                     ILTFIKEDEKVAEKLKKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITN
                     EIKIDEEIRNINKDYENIILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDL
                     VDMTLEQYFETFNIDFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEH
                     VEEVRVGDYWSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLS
                     TSRVLYTNRAFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNH
                     IKEVKMISRTLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISH
                     ELKTPLNNIYSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQ
                     VKSDIYKIKAVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERI
                     ILNILSNAIKFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENS
                     GISNEFGSGIGLCLTKSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYN
                     DNIEKFEIEFFDIYK"
BASE COUNT          957 a          494 c          243 g         1331 t
ORIGIN      
        1 ataccttcct atagatacat atcttaccta tataaaaact tattctatat aaaaacgcca
       61 taaaaaatct tttgcttctt ctgcataatc tatatttata cgtttactag tttttatatc
      121 taactcatct ttactaaata ctttttttcc tttatcatca taataaaaat cacttatata
      181 taattcttca cccattatac tcttactatt tagacttcta tctattttta aagctttgca
      241 aagttttcct ggtccgttag taatattttt tacttgatat ttactcaact cagtgtaagc
      301 tttgttgtat ctattcatag atatctcatc taaagaagtt ataggctcta cccctcttat
      361 taatacacac tctggcacat tttctatatt tgcagataaa ttcaagcaat tatacatacc
      421 ataaattaaa taaacatata tatgtccacc atctaaatat aatggcttag ttctatctgt
      481 ctttttattg ccatatacat gtgctccttt atcatttaca cccatgtatg cttctgtctc
      541 tatgattttg gtaacaatta ctttattttc atatttcctt attagatatt tacctaatat
      601 tgatttggct aaatcaattc cattttttct aaaaaaatcc ttttccaaaa tttcacctca
      661 taaaatttat ttaaaacgca tcaaatacaa ctaaagtaat aaataaaatt aactcttatt
      721 aatgtgatat atataaacaa taaatttaga catttattat ttatatatat caaaaaattc
      781 tatctcaaac ttttcaatat tatcattgta atttgaatta tcatatactt cttcaacaac
      841 ctctttaatt gggaatataa cttttacatt agttccttct cctaccttac tatctatgta
      901 aatcttccca ttttgaattt caatcaatga ttttgtaagg cataaaccta taccacttcc
      961 aaattcattt gatataccac tgttttcaat ttgtttaaat cgattaaata tatcctttaa
     1021 tttttctttg tctatgccca ctccagtatc ttttataatt atctctactg tttcgtcttt
     1081 tttatatata cctacaagga tttctcctcc ttctaaagta aattttatag catttgataa
     1141 tatatttagt attattcttt ctatcgattc tgggtcaagc cctaccataa cagtttcttc
     1201 atctgtatca aaaattaaat ctattccttt agacttagca taactactaa tagatgtaac
     1261 tattctttct gttatgtcaa taatatcaaa attaactgct ttaatcttat atatgtctga
     1321 cttaacttga gaaatattta ttatattatc tattaatctc aataatctaa acatattttg
     1381 tttagttatt tttatatgtt cttttagtat atcctgaaaa tcaattattt ttcctttttt
     1441 ataaaggttt tcaataagtt gaactgaact atatatatta tttaaaggtg tcttaagttc
     1501 atgagatata ttagcataaa actccatctt catttggtca tactgtcttt gttttattaa
     1561 tgctttttta agtctttcag actctctttg cacacttaaa tctttaataa ttctcactct
     1621 acttcttttc tgtccaaagt ataatgttct tgatatcatt tttacttctt taatgtgatt
     1681 attttcattt actatatgga tactcttctc tctttctcct atatttaaat tgtttatgta
     1741 tttttttact aatatatttc tatatttttg tgttgtaact cctaatacat cactagaaat
     1801 tccgaacaac tctctaaagg ctctatttgt atatagtact cttgatgtac ttaaatcctc
     1861 aagaaaaata gcctctggta taacatccag taatttttca tatattattt tttgttcatt
     1921 tactgttcta agaatattta tctctttagt tatatctcta aatgtaagca atttaaagag
     1981 tttattatct gaccaatagt ctccaactct aacttcttca acatgttctt caccttctga
     2041 gtcagacatc acaaaattaa ctatacagtt tgctgtgtca ttgtaactta ctatttcagc
     2101 tttcttttct tgtgttagat ttaatctttt aaattcccta ttgtcaaaga aatctatgtt
     2161 aaaagtttca aaatattgtt ctaatgtcat atcgactaaa tcacttgaaa cacatttaaa
     2221 actcttttca tattttttat ttatacttat taccttattc ttctttatgc caccttcagc
     2281 acttcttaca agtataggag atggaatatt tagtataatg ttttcatagt ctttatttat
     2341 atttcttatt tcttcatcta ttttgatttc atttgtaata tcatttaaac ttattaagta
     2401 attgctttcg tctttattat tacttaaact taacaaataa attttatcat ttaactctac
     2461 cagtttatca attatactta tatcgttatc aaatttcttc aatttttcag caactttttc
     2521 atcttccttt ataaaagtta atattttata ataattattc atatcctttg aaaattcatc
     2581 tttaaattta ttatttgtgt aacttatatt aaaatcatag tctgttacta tccatacatt
     2641 tggtattgta tccagagctt gtctaagtaa ttcttttctc tggtctatgt agttttttaa
     2701 tgttctttgt ataatcatat ccttttctat tgctaaattt atctttccta attgctctga
     2761 ctttttactt aattttttat tgattgaatc aagttcataa tatggataca ctactctatt
     2821 attaagactg tatacatata cataataagt atggttacat aatagtaaat atattactcc
     2881 tattaatcta tctgaatcta ctatattaat cttagaaatt gctacaaaac atataatagt
     2941 tgtaaaaaaa acaaaatatg ttaaatcttt tattaactta tagtgctcaa tgttactatc
     3001 aagtttattc aacaaatata gtaaa
//
LOCUS       DAMJZE010000275         3017 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJZE010000275 DAMJZE010000000
VERSION     DAMJZE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.280"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(56..1003)
                     /locus_tag="PC541_003704"
     CDS             complement(56..1003)
                     /locus_tag="PC541_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841119.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(1031..1522)
                     /locus_tag="PC541_003705"
     CDS             complement(1031..1522)
                     /locus_tag="PC541_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841120.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(1965..2858)
                     /locus_tag="PC541_003706"
     CDS             complement(1965..2858)
                     /locus_tag="PC541_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2841121.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         1008 a          427 c          266 g         1316 t
ORIGIN      
        1 attttccttt aaccaaatgc tattttaata agtttaagtt ttagctttaa aatacttaat
       61 aatacaataa aagtgatgta tttacaaata attcttctgg atttaattca tttattgatg
      121 tatgttcaat ttttatattt atatttttca tatcttcata aataattcca actggagaac
      181 aaatgaaaca agaaaatcca aaagtttttg ttattttttt taccttatta tcaccaaaac
      241 ttatttgctt tttacaatcg tttatatttt cattgttatt ttgtatatca tttttataat
      301 acttatcttt tttaatgtca ttagacttat ttactgtttt aaaattactc ctatttttac
      361 aactatagtc tatttcaact tctatatacc caaaaactat caagttacat aatttcaaat
      421 tactaccttt gtttataaga tttttcatag gtttaacatc aaatatttta ctatttacat
      481 ttacttttaa tatatctctt agatttttag gagcttttaa agtattactt atattcaatt
      541 gtttgaatac aattttattt acattagtat aaactctatt actttctatt gatattcttg
      601 ttgggaattt atctagatta cacttataat aatagtatat attagcacta ttctttataa
      661 tcctatgtgc agaatttggt aaaactacac tcttaaaaga tattattttt ttacaacttg
      721 catctattct ccctatgtaa aaaccttgct gtgggtctaa tccacgtttt ataatgccat
      781 taacttttac cgtattattt ataaactcta tatatttaga aatataatct ttgaaaaata
      841 tatttccagt tttgtaccta cttgtatttg taacaataat agtgtaggtc aaaatctcaa
      901 atacagatgc acatttttta tcaacatatt tttctacttt tataatactt ccatttgcat
      961 catttgaaaa tacatcatta aaagtagttt tcgttattct cattgcatcc acctttctat
     1021 aatttaaaga ttactctatc ttggaactat tttcataact tataattgca gtaacactaa
     1081 taaataaatt atcatctaaa atagatgaag taatatcttg tataagatac tttaaattaa
     1141 ttttatcttt attttctatc tttctaggca tttgtataaa tgtactaaag ggtattttta
     1201 atttaaatgt atttttttta ctagaatttc tatttctttt attgcctgca taatctaata
     1261 ttgcatcaat atctatatca ccaactaaaa tcaacttctt tcctgtaagt tgttcatctt
     1321 ctaatgatat acattctata gtatcaacaa gacttatatt gttaatattc atattgtaaa
     1381 catatgtctc taatgaattt attacagttt ttttagaagc taatttaaaa tttttatatg
     1441 tattaaattg agataaataa agagtctttt ctagtttttc acatctttca aggttgtata
     1501 ttaccttatt attttcatac ataatcgcat caatctccct gtatctatat attaatacta
     1561 ttcataaaaa ttatttcaaa ttaccatttt ttattattca ctatctagtt gttatttact
     1621 tttaagaaag aatccttttt tacttcattg atttcaattt acaagtaaaa gtctttctga
     1681 aaaaaaaata tatatatctc tattcacttt tttataagta attataattt ttatagacta
     1741 acggtcagcc ttaagggaaa aggtgttctt taagtgcaga atttagataa agtaaataat
     1801 ccttttataa ataattccaa gttttaactt aaataatttg atttacagta taaagtaaat
     1861 tttgaaagat aatgatatcg atttaataat tacaagtaaa agcatgtcaa aataaggcta
     1921 tgattttaaa aatcatagcc ttatcaataa aaatttaatc aaagttacat ttcactttga
     1981 atttcactgt ctattaattg aagattatac tgttgaatta attctattag acgagctgca
     2041 tatatactat tgccattttc atccttcgca gtactataac ctgccttttc taatgcttta
     2101 gcttgatata tatacgtttt tgcatcaaat acaccattgt ttttatacct tttatttgtg
     2161 gctaaaaatt ttgcatggtc ttttatagat tcatttttat catcatatat tctaaattta
     2221 tcatctataa ttgtatctaa atgttctttc gtttctaaag tcactgactt gcctttccaa
     2281 taagcgtcag cctttatacc aaatagattg ttatattgtt ttgccaaagt tgacttgccc
     2341 cagctagatt ctattatagc ttgtgcaata gttatagatg gaagtatttt ataatcctta
     2401 taattttgta ttgcttctgt tttaatttca tcaataaact tcatatattt tgtatcagac
     2461 ttcaatcttt ctggttttat accataatcc ttaagattgt ctatataatt atcaaccaat
     2521 cttttttgtc tattactaaa ttctaactca tccaaaatat cacttaactt atttatttta
     2581 ttatttttag aaaaattctt aacaaataaa tctgatactt cttgtatctg tgaatcctta
     2641 acgtttttta agttattttt atttaacaca gcaacaatgg aagctacata cttccagtta
     2701 acttgtgcat tatttttact tgctaaatca gcatatttta tatacttact gacatttata
     2761 tcatcctttt ttatctctct agatgaatat tccttcacta atacacctat taaaattgat
     2821 attatcagta ttataacaat gaatttttta ttcttcataa atattgtcct cccttatact
     2881 atctgctgaa tataatatac cttacttttg tgacatattc tttacgaaat tttgaaaatt
     2941 ttatgaagat ttatgaaata tatttaatta aaagcactac tattgttata atatatagtt
     3001 ttctatcaca caaaata
//
LOCUS       DAMJZE010000276         2996 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJZE010000276 DAMJZE010000000
VERSION     DAMJZE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.281"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            70..2427
                     /locus_tag="PC541_003707"
     CDS             70..2427
                     /locus_tag="PC541_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2841122.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2513..2830)
                     /locus_tag="PC541_003708"
     CDS             complement(2513..2830)
                     /locus_tag="PC541_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2841123.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1144 a          329 c          485 g         1038 t
ORIGIN      
        1 aaattgagaa ttttataata tatttgtaga tttgagtata aaaaagtgct aagttgaaag
       61 gagtaaaata tggataaggt taatttagaa aggtatagtt gtgatataaa tgttggttta
      121 aataaaaatc aacttcaaag tagaatagat gataatttaa ttaatgcatt tgataatgga
      181 aaaaccaaaa catataaaca aatttttaaa gacaatatat ttacactttt taatctcata
      241 aatattgttt tagtctgttt attagtttca gttggttctt ttaaaaatac cttatttata
      301 tttattgctg taattaatac tattatagga gtaatacaag agataagagc taaaagatta
      361 ttggacaatc tatctgttat agtttcaaat aaggtatctg tcataagaga aaaagagaaa
      421 aaagagattg atgtacaaga attagtatta gatgatatta tgattttaaa aacaggtaat
      481 caaatttgtg cagattcaaa agttttaaat ggaaaattag aggtaaatga gtctttggtt
      541 acaggtgaat ctgatattat tataaaaaat aaaggcgatt ttttatattc tggaagtttt
      601 gtagtttctg gagaagcttt tgtaagagtt gaaaatatag gaaaagataa ctatgcaaat
      661 aagatagcta atgatgcaaa gatttctaaa aaacataatt ctcaacttag aaattcatta
      721 aatgcaatat taaaaattgt aggtataata ataattcctt taggaattat attgtttgca
      781 aaacaacatt ttggaaatgg agattctata gctacatctg tagtaggtac agtggcagct
      841 gtaaatggta tgataccaga gggattaaca ttacttacga gcattgctct agctgtagga
      901 actataaaac ttgctagtca taaaacttta gtgcaagaac tgtactgtgt agagactctt
      961 gctagagttg atacactatg tcttgataag acaggtacaa ttacagaagg taaaatgcaa
     1021 gttgatgata tagtaatgtt ggaagaagtg gatattaatg aaataatggg gaatatatgt
     1081 aattctctca aagatgataa tgcaacacta aatgcaataa gagataagta taatgtttta
     1141 gatacgtata aagctaagaa tttaatacca ttttcttcag aaagaaaata cagtggggtt
     1201 acctttgaaa ataaaggtac atatttatta ggtgcatttg aatttatatt taaggaaaaa
     1261 aataaaaaac ttaggataga agttgaaaga tattctaaaa aaggaaacag agtaatagtt
     1321 ttagctcata gtgattctta tatggaagat aataatattc ctgaagatat aaagccacta
     1381 gcttttatat tgatattgga taaaattaga gatgaagcta aggaaacttt agaatttttt
     1441 tacaatgaag gtgtagatat aaaaattata tctggtgaca atcctataac agtaagtgag
     1501 gtagctagaa aagcaggtct taaaacagct agtaatttta ttgatgcaac aacacttaaa
     1561 aatgatagtg atatatacaa agcagttgat aaatacagtg tttttggaag ggtaagccca
     1621 caacagaaaa aacaaatgat tttagcatta aaaaaacaaa atcatacagt agctatgact
     1681 ggagatggtg taaatgatgt gctagcactt aaagaagcgg attgtagcat tgccatggca
     1741 tctggaagtg atgttactaa gaatgtgtct aatttagtct tattagattc aaactttgca
     1801 tcaatgccaa aggttgttat ggagggaaga aaagtaatta ataacattca aagagcatct
     1861 tctttgtttt tagtaaagac aattttttca ttcttcttgt cgttattaac attattcttc
     1921 ttcagtagac agtatccatt tgttcctata caattatcac tcattggggc tgtaacagtg
     1981 ggtataccat cattcttctt agctcttgaa gcaaacaaaa gtagaatttc aggaaatttt
     2041 ttactcaata tattaaaaaa tgctttacca ggagcactat gtgtgacagt aaatgtaatt
     2101 attgtttaca atatagttga atatttaggt tatagttcaa gtgtattttc cacaatgtgt
     2161 gctatattaa ctggtgtatg tggtctttta atattaagac aacaatgttt gccatttaat
     2221 aaagaaagat tatttttaat agtaagtatg actattgcat ttgttattgg gattctattt
     2281 ttaggtggat tattctcatt tgtagcattg agtagagaat taattctcat aactatggca
     2341 ttagcaatat taatgccaat tatgataaaa gttatgttat ttgtcttaga tgagatatta
     2401 gagacaattg ttccagattt aaattaattt taaaatgata taaataagag cagttttagt
     2461 tgaactgctc tttttagatg tgtttaattt tatgtaagaa tatgtatttt tattaatcag
     2521 ctatatatga gcaacagtag tcaacaactt cattaacctt taagtcaaaa cttgaatcac
     2581 taggaacatt aaatttttgc ccttctttat aagttacaaa ctcattgctt ccaggcagtt
     2641 ttacatccat acttccagca agcatctcca ttatttcttc ttctctagta gaaaaagtgt
     2701 attctcctgg caacattaat cctaaagttt ttctttctcc attaggcagt attattactc
     2761 tgctacttac cttaccatca aaataaacgt ttgccttctt tactgcagta acattcttaa
     2821 attcactcat tagataaaac ctccaaaatt atttgtacat atatattata taaaaataat
     2881 gtaaaaaatg ctatatattt acaaaaaaat ttagtattta ttaaaaaaat taataagaat
     2941 aaaaaagaga tgaaattgct taaattgata aaaaatataa aataaattgc aaagta
//
LOCUS       DAMJZE010000277         2918 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMJZE010000277 DAMJZE010000000
VERSION     DAMJZE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.282"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            80..1189
                     /locus_tag="PC541_003709"
     CDS             80..1189
                     /locus_tag="PC541_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2841124.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1566..2750)
                     /locus_tag="PC541_003710"
     CDS             complement(1566..2750)
                     /locus_tag="PC541_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841125.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1111 a          441 c          402 g          964 t
ORIGIN      
        1 tttactaaaa atacttacaa atatgatatt attaattggt taataaaaaa gaaaattttt
       61 catgaaaggt aagataacaa tggatttcaa aagcttaggg attagtgaaa atacaataaa
      121 tattctaaaa aaatcaggaa taacaacacc tacacctata caaaaagaaa gtattaaact
      181 aataaaagaa ggaaaagatg taatagcaga agctcaaact ggaacaggaa aaacactagc
      241 ttttttactt ccgatatttg aaaatatatc attagatata aatgatatac aagtacttat
      301 actttctcct acaagagaac tagctattca aataacagaa gaagctatga aacttaaaga
      361 atctaaagat gtaagtattc ttgcagcata tggaggaaaa gatattggtt ctcaaataaa
      421 gaaactaaaa ggaaatattc atatgattat agctacacca ggtaggcttt tagaccatct
      481 aaatagaaaa acaatagacc ttagtaagtt aaagactttt gttttagatg aagcagacca
      541 aatgttgctt atgggattta aaaatgaagt ggaagctatt ttaaaagaga cttcaaacaa
      601 aaagcaaaca ctatgctttt ctgcaactat aaattctcaa gtaaaaaaat tagcttatag
      661 atacacaaaa aatcctgttg tagtttctat tcaaaaagaa gaaatcactc ttaataatat
      721 aaaacaagaa gtagtagaaa ctacagatag aaaaaaatta gatgccttat gtaaggtact
      781 agatgaagat aatcctttta tggcaataat attttgtaga actaagagaa gggttgacaa
      841 tcttgaagaa gctcttgcta taagaggata taattgtcaa aaacttcata gtgatattgc
      901 tcaatcaaaa cgagaaagaa taatgaaatc atttagaaat ttagatatac aatacttaat
      961 agctacagat gtagcttctc gtggtcttga tatatctgga gtaagtcata tatataatta
     1021 cgatttacca gaaacaccag aagactacat ccaccgtatt ggtagaactg gtagagctgg
     1081 tgaagaaggc tatacatgtg cttttattga ccctaaaaat gaacgaatgt taagtgaaat
     1141 agagacggct atagaatcta aaattagtag aagaattata gaattataat ttatagattg
     1201 cttaaaatac caatgccaca caaacactgt aatatataga aagctacaga aaacatgtat
     1261 caatctatac tttgctgtta tactatttta tattaaagaa aaaagaggct atccaaagta
     1321 ggattagtct cttttattgt caaaataatg aaaatatatt tgacttcata taaaaaagag
     1381 tgccttataa actaaaagtc cattttgaga taccctcttt ttatctttta ttcttagttt
     1441 tatattgctt gtttatagtt gtttaatatt agattttgca aataatctta ataaacatgc
     1501 agatatatta tctatagtat tcttatttat agtattctct ttagcaatat tttttgctac
     1561 tacatctaat ttacattttc atttaattcc gctggccatt tagcttctgg attttccttt
     1621 gcaaacttct tattcttata atgtcctagt gtaagaaatg gaattactat agtgaataga
     1681 ccaagataac cacaatatcc atatccatat ttaacaatat tacttaatcc tgtaagtgat
     1741 ataagcattg acactaccat tattgcacaa gctgtaaata cccttctttt cattcttgaa
     1801 gaaaggaaag aaagtgcttt atggtcttca aatctattta caagaccaaa tatagtagtt
     1861 actcctgtag acattaaaca aaggaataat gcaacattat aaattatgta tatagtatga
     1921 tttccagatt gacctgcaac ataaagagta gggagtgttg tttgcccagc agcaataatt
     1981 tctctatacc atcccattag catagttaca gcaagtccta atgcaattgc attcataaca
     2041 aatgaaatta tcattgcctt agtagcttct tttgagttct taagaagtgg tcttgaacat
     2101 cctgcaagtg taggtataac aaccgattga aaacctgcat aaataaaagt attaagtatt
     2161 ggcttgatat agccaggtgt gaaactcgtt ctatttgata atatttcagt tatttctggt
     2221 actttagctt ttataccaac aataaaaata gtaagagtac atactagaat agctattgtc
     2281 attgctgtag ctgctttaga tacaagcata gcaccaaata tagtaaagac aagaagtaca
     2341 actccaataa taaatacagc tacaaaataa tttactccta tactttgaaa tactgctgct
     2401 gcacctgcta taacagcact tacagctatt aaaaccatta gatagtaata tatctcccat
     2461 attatttcaa gttttggata tggtctccaa agctcacaaa ataaatcctt ataattccta
     2521 caattgttat tattatacat tatgataact tctctcatgg tagcagtaag tataatcatt
     2581 gctaaaattg ccataagtgg tgctgtccat ccatactgca catagtactg agtttcttga
     2641 ttccctgtag caaatcctcc tcctgcatgt gaactaaaaa gaacgcttgc aacaccaaaa
     2701 atagctaaca tagagcccgt tacttttgct ttctttacac ttttttgcat gaaatcatcc
     2761 tccctaaaat gatattgaaa gtttttattt taacacttta ttgataaaac aataacattt
     2821 ttgctcatta aattcaacta tatttttcca atgactttac attttcaact tatttccacc
     2881 tttatattaa atatttttta tatatttatt ttattttt
//
LOCUS       DAMJZE010000278         2822 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJZE010000278 DAMJZE010000000
VERSION     DAMJZE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.283"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            296..694
                     /locus_tag="PC541_003711"
     CDS             296..694
                     /locus_tag="PC541_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2841126.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(911..1120)
                     /locus_tag="PC541_003712"
     CDS             complement(911..1120)
                     /locus_tag="PC541_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841127.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1152..1361)
                     /locus_tag="PC541_003713"
     CDS             complement(1152..1361)
                     /locus_tag="PC541_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841128.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1660..2079
                     /locus_tag="PC541_003714"
     CDS             1660..2079
                     /locus_tag="PC541_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841129.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2307..2762
                     /locus_tag="PC541_003715"
     CDS             2307..2762
                     /locus_tag="PC541_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841130.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
BASE COUNT         1075 a          350 c          350 g         1047 t
ORIGIN      
        1 tctttttctc ttttacagac aatcaaaaag caatcttagt ttatcgaaat aaatttatac
       61 aatctattca tacacccctt tacatttata taacaattaa acctattgtt atatatacaa
      121 atcaaaataa accatatatt ttcaactata atatgttaaa atcatattat gagcatatta
      181 atttaagcaa aatgtcattt agactttaat ttagtagttt tatattataa atttatatat
      241 ttttaatttt aaatgtcatt tagacattac tcataaaaag aaaggagtgt attttgtgga
      301 tactttagga aaaagaattg cttatttgag aaactccaaa aaacttactc agcgcaaact
      361 tatggatatt ttaaaatttg aaaacttagg caagtatgaa acaggagata gaaaacctaa
      421 ttgtgatata ctaatgtcaa tagctgatta ttttaatgtt acgactgact ggcttctcta
      481 tggtaaagag aaggtaaatg tcaatagtag tgtaaaagaa gataaagaag attacttaca
      541 tgttactaat gatgaaatga cgatattaaa tctatacaga caactaaacg aacgtgataa
      601 aataaaaata gaaggaatac ttgaacttaa gatatctgaa tataaagact taaaaaagca
      661 ttcctctaat aataatgaag ataaaatggt ttaaacatac ataatttgat acgattttac
      721 atcatctatt aaccttcttt ttttgtagta tattactaaa atatggatat tggattattt
      781 aaaaacaagt atagtctaat ttcaagcaca tttataatat ttagattatc ttatccatat
      841 tttagtttaa gtactttgaa aaatataata catctctatg tataacttat attcctaaga
      901 aatgcattac tcatgttttt aattcttcca acatctttct atacacaatt gtacatcctt
      961 cataattccc ctctttaaga aggattgaac atgtactaga atatttagtt aaagtattta
     1021 atctttccat atttaagctt tttatattac tgagtgctgt aataaaatca tcaattgctc
     1081 tacaacactc tataacccca tctgaatcca atttacacac aagaatattc acctacttta
     1141 tatactattt attaatcatc ttctgaatat tctatattaa gtaattgata aattgatgat
     1201 ttatattttt tacctgttct tcttccattt aaaatagctg acaaatagtt ttcattaaca
     1261 cctattttct tcgctaattc cctttgagtc atatttaatt caataagtct ttgcttgact
     1321 tgcactccca aaaaaggtat ccattttttt cgagcatcca tttatcaact tcctttctaa
     1381 gtgatttata tttactttta tattatttta gcttacacac cccctctatt tagtatatat
     1441 ttttaatatg tacacatatt aagtatataa atttatttaa tactaaataa gattttttga
     1501 ttactttgtg ctaaaattag gatgtaaatt cgtatcatta agttaattat aaagtcattt
     1561 tgaccttaag tcaatgcttt ttatgtcatt ttgaccttaa atttgaaact atttacatct
     1621 taaaaagtct ttttgacatt aattgaatgg agagtgttta tggatacttt aggagaaaga
     1681 attgtttatt tgagaaaagc aaaaaacctt aaacaatatg aactagaaga aatgttgggc
     1741 tgtgacaact taagtaaatt tgagagaaac attagaaaac caaattatga aatattaaaa
     1801 tctatagctg agatatttaa tgtttcagta gactggctat taaatggaga taatctatca
     1861 cataaatcag atttaatttg cgattcctcc tcaaactatc cacttaatag cataaactct
     1921 aatgaaataa agcttttaaa caattttaga aagttaaatg attatgataa agccaaaata
     1981 gaaggtatga ttgaattaaa acttcatgaa tatgaaaaag agaaggattt aggaaaaatt
     2041 gagtataaca aaaataaaga tgagaaaata gataaataat ttttatgttg cttttgatac
     2101 gattttacat catttctttg ttctaatatt tttatttcat ttattttctt ttttataatt
     2161 ttatgataaa ataattagta tattttgctt tacttgaatg taattattta tacaaaagtc
     2221 aaacataata taaatgtagt aaatttttag tttcaagtaa tatctcgtta ttataactaa
     2281 atatttatta aaattaggag gataaaatgg gcttaagaga caaattcgcg caatcatttg
     2341 ctagatcaaa aactatgagt ggccctgaga aaaaggctaa tgaaataatg ggtaaattgc
     2401 ttcttaagaa agctatatta cctatcgttt taatgttcgt gataataatc gctggagcta
     2461 tgcttaaaat caattcttgg gttacacttg gcataaattt agttattgct gttggtgctt
     2521 tcttctacat aagaaattct agtaaaaagt atcagaactt taaaccttat gttggcaatt
     2581 tgattagttt agagaaaaaa ggtaaaaaag agtacgttgc tattataaaa caaggtaagt
     2641 taccagttaa gttacaaata gcttatggtg gtgaagacct tgaacatgtt aagaaaaatc
     2701 aaatggttca aataagttat aatccagatg ctaaaatagc tattttggtt aatagacaat
     2761 aatttaataa aaaaaaggag ttgtcttaaa attgattttt tctaatcaaa tttaagctca
     2821 ac
//
LOCUS       DAMJZE010000279         2816 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMJZE010000279 DAMJZE010000000
VERSION     DAMJZE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.284"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            215..1036
                     /locus_tag="PC541_003716"
     CDS             215..1036
                     /locus_tag="PC541_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2841131.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1115..1326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1413..2729
                     /gene="argH"
                     /locus_tag="PC541_003717"
     CDS             1413..2729
                     /gene="argH"
                     /locus_tag="PC541_003717"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2841132.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         1152 a          250 c          550 g          864 t
ORIGIN      
        1 aatttctagc attataagac tataaattga aaatattcag aaaaatacta caagaaacga
       61 tttattttgc tcttaaaata agttaaatga atgttattaa ttttgtaaat tgaaatagtt
      121 taagtataaa gttatgatat aatctaataa gggtaattct ttagattata ttaaggaaat
      181 ataggtatac aataaaaagt tatggagttg gattatggga tataaattaa ttgtaacaga
      241 tatggatgga actttattag gcaataatca taaagttaca gatgaaaata aaactgcact
      301 acaaaaagtt ataaaaagcg gtattaatgt aactttagca acaggaagag ccttcgattc
      361 agctaaatgc aatgtagatt ttcttaaaga ggacatgcca ataatagctt gtaatggttc
      421 attaataaga gagcaaaatg gaaatataat atattcaaat aagattgata caagaacatg
      481 tttaaacatt ttagatgtat tagataaata cgatatttac taccaatgta atagtataga
      541 ttctatgctt tcgaaaaaaa tagaaggacg tgaagataga ttaagtgtat ttttaggctc
      601 tgaaacagaa gtaattgtaa aagatgattt gagagaagaa atctttaaaa aagatatact
      661 aaaatttgta atcatagaag aaaaaaatcc atctattttg gatgaaataa gaaaagaatt
      721 gagaaaagtt caaggaataa agataacctc ttcatggcca aacaatattg aagtcatgaa
      781 tgaaggtgtt gataaaggta atgcagttaa aattttagct gaaaaaatga atatagacag
      841 agaagatata attgcatttg gagacaatta caatgacata gaaatgataa agtttgctgg
      901 tcttggggta gctatgggaa atgcagaaga attgattaaa caagaagctg attatgttac
      961 agatacaaat caagacagtg gagttgcaaa ggcaatttat aaattcctag agttaaaaga
     1021 atcacttagc tcttaaaaca gtatataata ggaaaaaata ggttgactat tttgtagata
     1081 gtaattataa tacaaataga taaaatataa aaatttagat tcagtgaaaa agagagtaga
     1141 tatagagaaa atctcagcga attgggggat ggtgtgagcc taatgataac actttatatt
     1201 gaaaagaact ttggagaata ctacttgaaa ttcagtatgg tagtaggtaa tctcgcctta
     1261 agagaataga gtgggtaaac attgtttacc aactagagtg gtaacacgga atatttcgtc
     1321 tcttggtctt tttgaccaag agatttttta gtttcattga gttacaagac ttataattaa
     1381 aatacaaaaa attaaaatat gaggtgaaag ttatgaaatt atggggtgga cgctttagaa
     1441 aagcggaaaa tcaacttatg gaagaattta ataaatcttt tggctatgat tgtgtactgt
     1501 acaaaaaaga tatcgaagga agtgtagcac atgttcatat gcaagtaaaa tgtggacttt
     1561 taacagaaga agaaggaaag tctataacag aaggtttaaa agggatactt gaagatgtag
     1621 aaaatggaaa gttagtattg gatggagagt atgaagatat acatagtttt actgagataa
     1681 atttaattca aagaattgga gatgttggca agaagcttca tacagcacgt agtagaaatg
     1741 accaagttgc agttgatatg agattgtatg ctaaagaaaa agcaaatgat ttggtggatt
     1801 taatatctga atttaaagct actataaaag atgttgctga caaaaaccca gtgatgatgc
     1861 caggctatac tcatcttcaa agggcacaag tagtgacatt taagcatcac ttaatggcat
     1921 attatagtat gtttgataga gatgagaaaa gaataaaaaa tgctatagaa atattagatg
     1981 aatcaccttt aggctgtgga gctttagcag gaactacgca tgatatagat agaagtataa
     2041 cttgtgaaca attaggattt aaaaaagtag tagataattt tatggatgga gtaagtgata
     2101 gagattattt actagagcta atgagtgatt tttcaattat aatgatgcac ttaagtagat
     2161 taagtgaaga gttgatactt tggagttctc aagaatttgg atttgttgag atagatgatt
     2221 tatatacaac tggaagtagt ataatgcctc agaagaaaaa tccagatggt gcagagctta
     2281 taagaggtaa gactggtaga gtatatggaa acttatttgg tctttttaca gtaatgaaag
     2341 gaataccatt agcatataat aaagacatgc aagaagataa agaaggtttt ttcgatagtg
     2401 tacatactct tgaaatgtgt atacaaataa tggatagaat gatagccaca ctaaaggtaa
     2461 atgaggataa aatgaaacaa gcagtaaaaa atggtttttt aaatgcaaca gaagttgcgg
     2521 attatttggt taaaaataat gttgctttta gagatgcaca tggtatagta ggaagtatcg
     2581 ttatatattg tgaagataat aaaaaagcaa tagaagattt aacattagaa gaattacaaa
     2641 agttttcaga tgcttttaag gaagatattt atgattttat cgactatgag agtatattaa
     2701 ataagggaat aaaaaagaat ttaaaataat gtatttattt ttaaaagctg taaatttgaa
     2761 tgttatcatt taaatttaca gctttttatt gtgtaagatt ttattatata aaagta
//
LOCUS       DAMJZE010000280         2808 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMJZE010000280 DAMJZE010000000
VERSION     DAMJZE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.285"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            1174..2445
                     /locus_tag="PC541_003718"
     CDS             1174..2445
                     /locus_tag="PC541_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2841133.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1096 a          349 c          349 g         1014 t
ORIGIN      
        1 aatttttcaa tactttttat aattattcta cttttatatt ttaatcatta aaactaaagt
       61 caatgttatc tggtaattat ttactacaaa tgataaaaaa taattatatt ttaaaggata
      121 aaatgctgta taaattattg gggtatttta cctatacttg ttacaaattt acataccact
      181 tagttaatat aaaacaaatt tcaaagagat tggacaacga ataaaaattg caatttatat
      241 accatatagt taatataaag catatttgtg tcgatatata gttctaacta agtataagat
      301 ttacatacct catagttaat ataaaacaca tattttacat gtttgaagat tttatcagaa
      361 accctattta cataccgcat agttaatata aaactattaa tagtgctatg ttgtttattt
      421 gcattaacaa gatttacata ccacatagtt aatataaaac atgtttagca ggagttatga
      481 tgataaaaca cattatttat ttacatacca cttagttaat ataaaacccc aaaataaact
      541 aagtattttc agtacttaca cacatacaac tctctcaaat ttgcagtgaa ccatgagtag
      601 tgcaattgat aacatttatc atacaccctc aatcccttac attgcaactg ttaaacccta
      661 ctttatcaaa aatatcgctc actgcaaaat ttatatattt ttattatatc ataaaattca
      721 aactataaaa ttaaaatgga ataatttatc aatttaagaa cacctacata tttgtaaata
      781 cttactatat tatctattct aatagttctt cttctagcta gaactaatat aaaaatcatc
      841 tctgaaagat taggacatac taatataaaa ataactatga atagatattt tcttgtttta
      901 aacgatatgg ataaaaaaac ttctgaaaat ataatcaaag ttctctttaa ataacattcc
      961 attttatgta agtaggcatt atatttttct taaatgtcac ttaaatgtca gttatatata
     1021 ataatagtaa acaactgtat ttcactaata tttgctatta tctaatagta tttatagttt
     1081 agatttgata taatacatgt ggtaaaattt tgatttgtaa aacaaaattt tacctacata
     1141 tattaaaaca aatttaaaac taaggagacc tatatgttaa aagtactaaa aggaaatata
     1201 atttttacaa aaacttcaga aactttcaca gtatttgaaa atagttatat tatgctggaa
     1261 aaaggtaaag taaagggaat atttaaagag atacctaagg aatacaaaaa cctaaaagta
     1321 gtagattaca ctgataagct tataatacct ggtatgaatg acctccatgc ccatgcctct
     1381 caatttaaaa atcttggaat ggctatggat aaagagcttt tgccatggct tgaaacttat
     1441 acatttccag aagaatcaaa ttataaagat atagaatatg caacaaaaat gtataaaaaa
     1501 tttgtaaaag aactttggaa atctggaact acaagaatag cagtgtttgc aacagtacat
     1561 aaagaagcct ctatgaaact tatggatata ttcaaagaag ttggtcttgg agcatacgtt
     1621 ggtaaagtaa atatgaatat gaattgccct gatactttat tagaaaacac tcaggattct
     1681 attactgata cagaagaaat tataaataag tatagtgata cagattctat tgtaaaacca
     1741 ataataacac ctagatttat accaagctgt acaagtgaac ttttaaaagg tttaggagat
     1801 ttatgtttaa aatataatat tcctattcaa tcacacttat ctgaaaatta tgatgagatt
     1861 gaatgggtta gaagtctaga acctgaatct aaattctatg gagatgcata taatagatat
     1921 aatttatttg gtcaaactcc tactttaatg gctcattgtg ttcattgttc tcaggatgaa
     1981 atagatttaa tgagagaaaa taatgttatg gcagttcact gtccagcttc taactttaat
     2041 gttggtagtg gtgctatgcc tatcagaaaa ttaatcgata ataatattag attagcactt
     2101 ggatcagata taagtggtgg acatacattg tcaatattta aagcaatggt atctgcaata
     2161 caattatcta aattatattg ggttaactct ggtaagaagt ataatttctt atctttatct
     2221 gaggcttttt atattgcaac caaaagtggt ggaagtttct ttggaaaagt tggtagcttt
     2281 gaagaaggtt atgattttga tgcattgatt attgatgatt caaatttaaa tcatgacaat
     2341 tattctatac ttgaaagact tgaaagattt atatatgttg gtgatgatag aaatatcatt
     2401 catagatatg tatgtggaaa actaattgaa gaacctaata tataattata ataaaaattt
     2461 taattatata ttatggttaa tttatacaac tataattaat aaaagagtat attttaaatc
     2521 taaaagctat aggttgaaac atactctttt tattttgttt atttatagct ttagttatta
     2581 atcatatata aatctacaaa acgtattcct agtaacttac attttttgat atctacaatc
     2641 ataagactat attattattg gattcagacc tcaaaaatat tgccttctga acattattat
     2701 attcaaagct atgttaattt acaatgatat atctaacatg tactgtaatt atcaaacact
     2761 tctaatctcc tatattttca tattaatgta atatcttttt tattaata
//
LOCUS       DAMJZE010000281         2794 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMJZE010000281 DAMJZE010000000
VERSION     DAMJZE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.286"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..254)
                     /locus_tag="PC541_003719"
     CDS             complement(<1..254)
                     /locus_tag="PC541_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2841134.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
     gene            complement(1064..2101)
                     /locus_tag="PC541_003720"
     CDS             complement(1064..2101)
                     /locus_tag="PC541_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2841135.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            complement(2198..>2794)
                     /locus_tag="PC541_003721"
     CDS             complement(2198..>2794)
                     /locus_tag="PC541_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2841136.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
BASE COUNT          943 a          426 c          361 g         1064 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtgta gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacactcctt tgaaaatatt tttataattt taattatttg
      301 ctaattttgt gtataaaaaa accacatagt gtacaaaact atgtggtaaa aacacgaata
      361 agccacatag tttaatctat gtggctctta tatagttaga tgaaaacttt ataaacaata
      421 tagccaacat agatacaatc ccaaaatttt agtagtctta aggggtctgt atctatgtta
      481 aaacgtaggt acaaacccta aaaatagtgg ctataataat aataatattt tctattgttt
      541 aataaagtat tcataattat ctctcccttt taatatttta attagtcaga aaattatatg
      601 tattctcgac ttgttataaa ttataaatta tacaataata aaagtcaact gctaaattta
      661 attaaaatac taaataatgt atactcagaa tacttttaaa ttaatgtatt gtttgaaaaa
      721 aaacgttatc ctaagttata agtgaatgtg gaaattttat caaaatattt actaaatatg
      781 gaataactag acatgtatat caatcaaata tcataaaaaa aagctgtctg gagaatatta
      841 aaattctttt ggacagctca agtagtgatt agaaagttat ataactttca taatcagtcg
      901 tcaatataga cgacttttat ggaaaatgaa taactttata tgcaatattc aaattgctac
      961 aataagcaca attttcaaag ggttagggtg gataagagta cttattatat aaagttttca
     1021 tattacctac ttcatattac ctattttttg aaattaataa gttttatttt gtagattttg
     1081 cattttcatc tgactttata tactttatat tatctgctga taacttttgc atgtcaagtg
     1141 cttttagtgt atcttcgtta attattattt cagtttcatc taatgtagta actggtatat
     1201 cgccaacaga ttttccttct aatacttcaa tagcaagttc tccagcctta taacctagtt
     1261 tttcataatc tattccacaa caagctaaag caccagaaga tacagaacct tcttcagagg
     1321 ctattatagg aatcttgttt tcattagcaa ctttagaaac tattggcata gaagaaacta
     1381 ttaaattgtc agtagggaca tataaaacat ctattttacc aactaaacta gaaatagctt
     1441 ggttaacttc acttgaagag ctgattcctt tttcaactac ttcataatta tttttcttag
     1501 cataatcatg tagagaatca acttggattt ttgaattaac ttcactagta ttgtatataa
     1561 cccctatttt ctttgctttt ggagtcaatg ttttaaccaa ttctaatgtt ttatcaattg
     1621 aaagataatc agatgtacca gaaacatttc cacctggttt ttcaagagat ttaacaagtc
     1681 cagcttctac agggtctgta acagcagtca ttattattgg aatatcttta gtagcattat
     1741 aagctgcttg tgcagatgga gtagatatag catatatcaa atcttttttg tcggatacaa
     1801 acttactagc aatactttgt gtagtaggca tatcattttg tgcattttgg aaatctattt
     1861 ttatattatc tccatcttta tagcctttat cttcaagtgc tttgatgaat cctttctttg
     1921 ctttatccag agatggatgt tccactaatt gagtaatacc tatatttttt tgttttttgc
     1981 tatctgtttc tttattttca tttgaagcat tggagccatc attttgagaa catccagtaa
     2041 gcattgatac accaagtact cctgctataa ttaaacttaa tattttttta ctttttatca
     2101 ttttattttc ctcccttaaa atttaataat tatatttgtt catatattca tatgatagat
     2161 tataaatatt tctgattaaa actaagattt taatttctta tactgcatat tcaaattttt
     2221 ctaagatttc ttcaactgtt aaatcttttt tttctttatc ttttacatct aatacaactt
     2281 cacccttatg aagcattatc aacctatctc catattgaag agcgtgtttc aagttgtgag
     2341 taaccataag tgtagttata tttttctctc ttacgatttt atctgttaac tctattactt
     2401 catttgatgt ttttgggtca agtgctgctg tatgttcgtc tagtaataag accttaggac
     2461 ttgctaagct agacataatt agtgatagtg attgtctttg accccctgaa agatattgta
     2521 ctttaatatc taaatatttt tttaaatcca aagaaattcc ttcaagtaag tgttccaagt
     2581 cattcgtttt atgacgaaga cattttttta agttcaataa tttcccttta ttaagtgcta
     2641 gagataaatt ttctctaacc gtcatagaag gacaagttcc aaggcttggg tcttgaaaaa
     2701 ctctacttac tatttgagtt cttttatact cagctaaatt tgttatagta gtactatcta
     2761 aagtaatttg accagaagat tcttttataa gccc
//
LOCUS       DAMJZE010000282         2778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMJZE010000282 DAMJZE010000000
VERSION     DAMJZE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.287"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            160..1083
                     /locus_tag="PC541_003722"
     CDS             160..1083
                     /locus_tag="PC541_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2841137.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1058..1885
                     /gene="panB"
                     /locus_tag="PC541_003723"
     CDS             1058..1885
                     /gene="panB"
                     /locus_tag="PC541_003723"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2841138.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1904..2752
                     /locus_tag="PC541_003724"
     CDS             1904..2752
                     /locus_tag="PC541_003724"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2841139.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1106 a          335 c          457 g          880 t
ORIGIN      
        1 tgaaaatggt aagatattta tggatggtat cttgttataa gaactatact ttaaatgttt
       61 ttaattattt tgtcttatgt aactcatatc ctcaggaatg agattagaaa ttaagatgtt
      121 gtatcacaaa gtggaggtga tactattaaa ataggcttta ttggttctgg aaaagttgga
      181 acatctttgg caaattattt tttatcaaaa ggttattgtg tttcaggctt ttatggaagg
      241 aatcaattat ctttattaga atcaacaaat ttaacaaaaa caaagattta cagcaatttg
      301 aaagatataa tttatgaaaa cgatatatta tttattacaa catcagatga ttctattgaa
      361 attatagaca aaaaactttc caaatttaat ttaaaacata aatacatttg ccacacaagt
      421 ggctctttaa aatctagtat attgttctgt tcaaaaaaag caggtgcatt aatttattct
      481 atccatccta tatttgcatt ttccaataaa aatactgaca taaaaaaaat gaaagacatt
      541 tgtttttcca tagaaggtga taatgaacaa gatgatttag ttattcaaaa tttcatagat
      601 gatttgggaa accaattttt tataagagat aaggatactt catccactta tcatttagct
      661 aatgttttag tttcaaattt agtattatct ttactagata taggagtaag ttactttatt
      721 aaattaggtt tatctgaaga ggaatcttta aaagctatca gtcctttagt aaaaaaaaac
      781 atagaaaata tacttgataa tggctttact aaagctctta ctggtcctgt ttctcgtgga
      841 gatataacac caataaggaa acatctatct gtattggata aaaaagatga ggatatttac
      901 aaaatactat ccttaaacct actaaaaata attgccttag gaaatgacaa ttcattaaaa
      961 ggtataaaaa atattactat agaaaatgca atcgaaaatt taataagtaa atctgaaaaa
     1021 tacaaagaaa tttacaaact tttaggagga aaaaacgatg aaaaatacca tacagacttt
     1081 taagaatgct aaatatgaag gaaaaaaact atctatgctt actgcttatg actactcaat
     1141 tgcaaaaata atggatgagt gtgatataaa tggtatttta ataggagatt cattaggaat
     1201 ggtcataaaa ggtgaagaaa acactctatc tgttaccata gacgaaatta tatatcatac
     1261 aaaagctgta aaaaatggcg taaaaaatgc cttaatcgta agtgatatgc cttttctatc
     1321 ttaccatgtt tctatagaag atgcagttaa aaacgctggt agacttataa aagaaggagg
     1381 agctcatgct gtaaagttag aaggtggctc aaacgttata aagcaaatag aaagcattgt
     1441 aaatgctcaa atacctgtaa tggggcattt aggattaact cctcaatcag taaattcatt
     1501 tggtgggttt aaggttcaag gaaataccag tgaaacagca agacaactta ttgaagatgc
     1561 taaattaata gaaaaagcag gtgcattctc tattgtcctt gaaggagtgc ctacgaaaat
     1621 agctgagatg gtaactaatt ctatttcaat tcctacaata ggtatagggg caggaattaa
     1681 ctgtgatgga caaattttag tttatcaaga tatgttggga atgtttggag attttgttcc
     1741 taagtttgta aagcagtatg caaatatagg agatataata aaagattcaa ttaaaaatta
     1801 catcctagaa gttaatacag gtgcatttcc acaagaaaaa cacagctttt caataaatga
     1861 atctgagtta gaaaaattat actaatatta aggatggtac aaaatgcttg taaaagaaat
     1921 taaattacta agaaatataa tcaaagattg gagaaaacat ggctattcta taggccttgt
     1981 tacaacaatg ggatttttac atgaaggaca ccaaagtctt ataaaaaaag ctgtaaaaga
     2041 aaatgataaa gtagtagtga gtatatttgt aaatccaact caatttggac caaatgaaga
     2101 ttttaatagc tatccaagag atatagataa agattttaaa tattgtatgg atagtggagc
     2161 aacagtagtc tttaacccat caccagaaga aatgtattta aaaggtaatt gtactaccat
     2221 taacgtttct ggacttacag attttctttg tggagctaaa agacctgttc attttggagg
     2281 tgtctgccta gttgtttcaa agtttctaaa tatagtaaca cctgataaag cttattttgg
     2341 agagaaggat gcccaacaat tagctgtaat taaaagaatg gtcaaggatt taaacataga
     2401 tacagaaata ataggctgtc ctataattcg tgaaaatgat ggattagcaa aaagttcgag
     2461 aaatacatat ctctctgagg aagaacgaaa atctgctcta atattaaata agagcttaag
     2521 tttagcaaaa gaagaattag taaaaggcaa tctaaatcca gagaatataa aagaattaat
     2581 aacagctaaa ataaattctg aacacttagc aaaaatagac tatgttgaaa ttgtagatag
     2641 tgagacatta caacctgtta agcaaataga acattctata cttgtggcaa ttgctgtgtt
     2701 tataggaaaa accagactaa ttgataattt tacatttgag ttaaatatat agtctataat
     2761 tttatataat atatctaa
//
LOCUS       DAMJZE010000283         2742 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMJZE010000283 DAMJZE010000000
VERSION     DAMJZE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.288"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            103..2238
                     /gene="rnr"
                     /locus_tag="PC541_003725"
     CDS             103..2238
                     /gene="rnr"
                     /locus_tag="PC541_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2841140.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1209 a          261 c          507 g          765 t
ORIGIN      
        1 aaaatataga aaattatgtg ataattaaaa aatgttttgt ctgaagaaat ataaaagaca
       61 atataaataa taatataatg aaaagtaaaa caggagggaa aaatgatacc aggacttaaa
      121 gaaagtttgc taggattaat aaatgaaagt gcatataatc ctctaaaaaa agaagaatta
      181 gcagaaatat ttgatataca ttcatcagaa atgccaatgt tttataattt tttaaatgaa
      241 ttagaagaag atggttatat ctgctttact aaaaaaggaa aaatagtatc tcccaatcaa
      301 atgggatact ttgttggaaa atttgtatca cacaaaaagg gttttggatt tgtagaatct
      361 gatgttgaat atacacagga tttatttatc tcaagtgaca atataaatgg tgctatgcat
      421 aatgatagag ttatggcaga aatagtagta cctgcaactg aagaaaagag agcagaagga
      481 agaatagtaa aagtaataaa gagagaaata actagaatag taggtacttt ccaatcaagt
      541 aaaacttttg gatttgtaac accagacaac aagaaattta ctaaagatat atatatacct
      601 aaaaaacatt ttagtggagc agtagataat gataaggtag tatgtgaaat tacagtatgg
      661 ccacaagagg atagaaaacc agaaggcaaa attatagaga tactaggtca aaaaggtgaa
      721 agaggcgtag aaatagattc tataataaga gaacatggtc ttccagaaga atttccaaag
      781 aaagtattgc aagaagcaga agctgtagct gttgagattc ctcaagaaga gataaaaaga
      841 agaagagatt taagagattt aaacatattt actatagatg gagatgacgc aaaagactta
      901 gatgatgcca tatcaataga agtattacct aatggaaact tcaaactagg agttcacata
      961 gctgatgtta cacattatgt aagagagaaa aataaacttg acaaggaagc cttaaaaaga
     1021 gctacatcag tttatttagt agataaagta attccaatgt tgccaaaaac tctttcaaat
     1081 ggagtatgta gcttaaatcc atttgaagat aaattaactc tttctatatt tatggaaata
     1141 gaccataaag gaaatgtggt taaacatgaa atatgtgaat ctgtaataaa ttcaaaagct
     1201 agaatgactt atacagaagt atctgatata ttagaaaagg atgatgaaaa acttaaaaag
     1261 acttttgaac atgttgttga agattttaaa aatgctgaaa ttttagctag gattcttatg
     1321 tcaagaaggg aaaaaagagg agctatagac ttcaatttcc cagaggcaaa gataattcta
     1381 aatggaaaag gcgaagttgt agatataaag ccatatgaaa gaagaatatc taataagata
     1441 atagaagaat ttatgcttat aagtaatgag acaattgccg aacattttta ctggatggga
     1501 ataccgtttg tgtatagaat acatgagaca ccatctttgg aaaaaatgga agaattgagt
     1561 aagtttatat ctacatttgg atatataata aaaggtgata aagaggaagt acatccaaaa
     1621 gcattacaag gtataataga gaaaataaag ggcaaaaaag aagaaggagc tatcagtact
     1681 ataatgcttc gttctttaaa acaagctaag tactcaccag aatgtagtgg acattttgga
     1741 ttagcagcta aattctactg tcactttact tcaccaataa gaagatatcc tgaccttcaa
     1801 attcatagaa taataaaaga atctttaaat aataaaataa gtggaaaaag acaagaacag
     1861 cttactacta tagtggatta tgcatctgtt caatcatccg agaaagaaag aaaggcagag
     1921 ttagctgaaa gagatgtaca tgatttctat aaggctcttt atatgaagga taaagttggt
     1981 caagaatttg agggtgttgt atctagcgta acttcatttg gaatatttgt tgagttggat
     2041 aataccatag aaggattgat aagacttgca aatatgaatg atgattacta tatttatgat
     2101 gagagttctt atagtgtttt aggagaaaga actaagaagt catataagat aggagacctt
     2161 gttaagataa aggttgaaag tgttaatgta gattttaaag aaattgactt tgagatagtt
     2221 gaaaaaatag aggagtagat aaaacagcta aaataagaga taacaagttg tagtatttat
     2281 gatatgtata aatatatagt gcacaatact ctataaaata aatgataaaa ataggtaatt
     2341 gagaaatatc taatggaaat agttaactga aagataaata attaagaaat atttaatgaa
     2401 agtagctaat tgaaaaataa gtaattgaga aatgtttaat agaaagagct aactgaaaga
     2461 taagtaatta agaaacattt aatagaaaaa gccaactgaa aaataaataa ttaagaaatg
     2521 tttaatagaa agagctaact gaaagataag taattaagaa acatttaata gaaaaagcca
     2581 actgaaaaat aaataattaa gaaatgttta atataagtgg ctaattgaaa aataagtaat
     2641 taagaaatgc ttaatataag tggctaattg aaaaataagt aattgagaaa catttaatag
     2701 aaaaagctag ctgaaagata agtaattaag aaatatttaa tg
//
LOCUS       DAMJZE010000284         2721 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMJZE010000284 DAMJZE010000000
VERSION     DAMJZE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.290"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(246..1058)
                     /locus_tag="PC541_003726"
     CDS             complement(246..1058)
                     /locus_tag="PC541_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2841141.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(1134..2552)
                     /locus_tag="PC541_003727"
     CDS             complement(1134..2552)
                     /locus_tag="PC541_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2841142.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT          954 a          485 c          277 g         1005 t
ORIGIN      
        1 tatatatttt gtatattaaa tacaattatt tataaagtct ttaataaaaa tttataaata
       61 gagcatcttt agaaaactat tataataaaa aaataagagg ctgtctaaaa atatagataa
      121 gccttttttc ataatcgtaa ttataaaaat atacttgatt tcatatgaaa aagagtgtct
      181 cataaactaa aatgtttact ttgagacacc acctttttta ttcttattta tactttgtag
      241 acatattata acatgcgttt cactggtatc tgaacttctg tcaaccagtc ttcttcactc
      301 tctttattcc atataccatc tatataagat tctctaggat ttccaaccac tttatattta
      361 ttatcctcta tccatttaaa tgcatctgcg taagtttttc ctaatgttga atatggacca
      421 ttgtgtaata tacaaactgc attttcaact gcatctaatt ctttaaattt taatatatcc
      481 gtatcttcct ttaaatcatt tatagcttga cacatttcaa catccatatc agtctctcta
      541 tattcaccat catgatatat attaaaacaa taatcaggaa ctgcacatgt acaccctgtt
      601 ttcatcattt cttcagccat tacatttgga aatagattaa aaagttcatc ataccccttt
      661 aacacttttc tcatagaagc aactttaact tttggtaaag acttaataat tacatcattc
      721 atattgactt ctcctttcaa attcataagg taattacgta gtttaattat tttatattct
      781 tcacttttaa ttacatccaa catttctttt tctttattca ttaaaatatt ttcaacttct
      841 tcaggatttt ctacagcttt ttttatctca gctaatgata aacccatttg ttttaaagtt
      901 attatctgat gtaatttagg taattgctct gttgtataat acctatattt tgtaaattca
      961 tctacatatt ctggttttaa caaccctata tcttcataat accttaatgt ctttgtagtt
     1021 attttattta ttttagaaaa cattcctata gaaaacatct catcacctgc gcattagatt
     1081 tgattaattt aagtttaaac tttcccctta ggttaaagtc aatacaaaat aaattataat
     1141 tgtattaatt cttcaattct atctccactc aataccaaaa tatcccctgt ttttataatg
     1201 gttgaacctt taggtattaa aacctgttcc tttcttttta tcataactat taatatatcg
     1261 tctggtatat tagaatctat tatactctta ttagcccatt tattaccctc atcaacattt
     1321 acttcgataa gtttagagct aatttctcca tcataatcat taaatgtttt taatacaggt
     1381 gtattatcat caactaaatc caactttctg gctacagttg gtatcaatga accttgtatt
     1441 cctactgaaa gcaacgctat aaaaaatatt atatggaata tgtcattctc tatggctact
     1501 ccttgagtaa cagcataaat tgcaaaaact actgaagccg cacctctaag acctacccac
     1561 gatatgaata atttttcttt atttgagaat ttaaattttg ctagaactat aaatgttgct
     1621 aatggtcttg caataactat catgaatata gatatggcca ttgctatacc aaatacattt
     1681 ggtaacttag aagggaaaga taacaatcct aacataaaga ataacattat ttgcataatc
     1741 catgaaattc catcaaagaa atgaaccaaa ctttttttaa gaggtatcct actatttcct
     1801 ataataatac ctgaaatgta aacacttaaa tatccatttc ctccaagata ctcgctaaat
     1861 gcataagaaa gtattgcaat agctgttata aatatagtat aaaatccttc tatctcgaag
     1921 tttgaatgtc ttaaaatata aacagataat tttgctagta taaatcctat agctaaccca
     1981 aaagctattt gttttattac cattggtgca ataagttcta ccccagaatt attcatcaat
     2041 gtcaaaagaa ttagtgtcag catatacgct attgggtcat tacttccact ttcaacttct
     2101 aataatgatg caagtgaacc ctttaagttt agtttttgag aacgcaacac agcaaacaca
     2161 gatgcagcat ctgttgaagc tgcaattgcc ccaattaata aaccttcaag taaactagtt
     2221 cctattacca tgtggcaaaa catacctgta accccagccg taattattac acccagtgaa
     2281 gacattaata tagaaggtat agcaacaggt ttagccatat tccagtttgt tccaaaccct
     2341 ccaaaaaaca ttataaatat tagtccaata gaacataatt gttttgtcag ttcatagtta
     2401 tcaaaataga ttcctaccat cccatcagac ccaaataaca tccctagtaa gataaaaatc
     2461 aataatattg gtactccaaa tttatacaat accttgctag aagttataca aatcaatagt
     2521 actaacccac ttataatcat taattctgtc atagcatcaa ctccatttca tataaatttt
     2581 gtagattaat ttacatctac aattttatta cttattcaaa tataacatga cataaatttt
     2641 ataagttatg gtacataaag ttcaacaaaa atttcacaat acattaaaat gtgagtgtta
     2701 tacttaaatt tatatatttg t
//
LOCUS       DAMJZE010000285         2714 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMJZE010000285 DAMJZE010000000
VERSION     DAMJZE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.291"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            137..1018
                     /locus_tag="PC541_003728"
     CDS             137..1018
                     /locus_tag="PC541_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2841143.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1079..2287)
                     /locus_tag="PC541_003729"
     CDS             complement(1079..2287)
                     /locus_tag="PC541_003729"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2841144.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2360..2612)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          903 a          460 c          338 g         1013 t
ORIGIN      
        1 attatgcaaa taatgtcata taagttacac tttagttatc ttagttacaa ctttgtcggt
       61 ttactttttc tactttcttc gatataatta taatatattt ttgatacaat tcgacaaaag
      121 ggggaatgct gtaattatgg taaaaaaaag aaaaaagaaa actgttacac ttactatact
      181 tgttgtaata gttattggta ttttttctat ggcatgggcg gacatgtcta gaaaaaaaga
      241 aatgaaagaa actcaagaaa gacttaaagc tgaaagatta gaaaaagaaa gaattgaaaa
      301 agaaaaaaat gaacctatta ttggtgcaaa aaaagaagct gtaaaagaat atggatatga
      361 cgcaaaaata gtttgggaca aacttaacaa atacgactat tcaaacaatg gagaaaagat
      421 agtctttctt acatttgatg atggaccatc tactactaat actccacaag ttctagatat
      481 cttgaaaaga catggtgtta gaggaacttt ctttattaag ggtgattctc ttgaaagaaa
      541 aggagctaat gaaattttaa aaagaacatt tgacgaagga aatgcaatag cccatcactc
      601 ttatactcat gactataaaa aactatatcc taacagaagc ctaaatctcg atacattcgt
      661 aaatgaactt aacaaaactg atgaagctat gaaaaaagtc ttaggaaaga atttttcctc
      721 aaatgtagtt agatgcccag gcgggtatat gtcatggaaa aatatggaac ctctaggaaa
      781 ctacttaaaa gagaaaaaca tggcatcaat tgattggaac gctttaaatg ctgatgcaga
      841 aggtaaaaag aaaaatgcac aagaattatt tgaacatgca aagaaatctt ctgaaggcaa
      901 agaaatggtt gttcttttaa tgcatgatac ttatggaaaa caagaaacag taaatgcatt
      961 ggaccaaatt ataacatatt ttaaagacaa tggttatcaa tttaaaattt taatataata
     1021 aaaaagcaag tttattgaat gtacatttta tactcaatag acttgctttt actttactct
     1081 attttactag ctcgattttt acaaattttt tctttcctat ttgaacaact aattcactct
     1141 cttttacaat agtgcttaaa tcctctactt tttcaccatt tacctttact ccaccttgag
     1201 aagctaatct tctaacttca ctcttactct taacaatctc attagaaact agtacttcga
     1261 ttaagtcaaa tccatcttcc ttaacttgaa ttgtttgtat atcttcaggt atttgacctt
     1321 tttggaatac agatttaaat ctttcttcag cttctttagc agattcttct ccatggtata
     1381 atgttactat ttctcttgct aagttcatct ttatatctct tggatttact gaaccctctt
     1441 tcaactgtga ttctatttta ttaacttcat caggatgaac atctgtcact aaattgtagt
     1501 atttaattat aagttcatca ggaatttcca tagatttttg gtacattatt cccgcttctt
     1561 catctattcc aatatagtta cctaaactct tactcatttt ttctttacca tctaatcctt
     1621 ctattaaagg catcattatt actatttgag actccatacc aaattctctt tgtaatgatc
     1681 ttcccattag taaattgaat ctttggtcag ttccaccaag ttctatatca gcttctaatg
     1741 ctatagagtc atatgcttgc attaatgggt agaagaactc atgtactgat attggcattt
     1801 gtccttcata tctcttttta aaatcttctc tttctaacat tctcgcaaca gtaatagttg
     1861 ctgctaattt tattacatct tcaaaattta acttagctaa ccactcgcta ttaaatctta
     1921 caatagtttt ttctttatct aaaactttga aaatttgttc ttcataagtt tttgcatttg
     1981 caagaacttg ctctgttgtt aaagcttttc tagctttaga ttttccagtt gggtctccaa
     2041 tttttcctgt aaaatctcca attattatta ctatttgatg acctaaatct tgtaattgtt
     2101 tcatctttct aagaactact gtatgcccta aatgtatatc tggagcactt gggtctagac
     2161 ccaattttac tatcataggc tttccttctt tttctgattt aataagcttt tcccttaact
     2221 ctttctcatc tattaaatca tctacacctt tcataattat tcgcatttgc tcgtctatcg
     2281 atttcatttt tcattcctcc taaaatattt atataatcta taatttttat ttttataata
     2341 aaaaaagctt tcatcctctt aaaaggacga aagcattgct tacgtgttac caccttagtt
     2401 catcaatatc tcacgatatt aatctcttag agtacaatta catatactcg tgtactataa
     2461 cgtgtactaa tccgatatct cttaatggtt gttatctttc agagatagtg ctctgagatg
     2521 tattcaaact aaatcaattt attccatttc accaaactgg aactctctgt taaataaaat
     2581 ctagccctac ttcttctctt catagcattt atatattcag attatttcat caattacaag
     2641 aaatttttct taacttactc ataatatatt attattttta tattttgtca acctttttta
     2701 ttattatata catg
//
LOCUS       DAMJZE010000286         2662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMJZE010000286 DAMJZE010000000
VERSION     DAMJZE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.292"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            262..2280
                     /locus_tag="PC541_003730"
     CDS             262..2280
                     /locus_tag="PC541_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2841145.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1096 a          306 c          437 g          823 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tataatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgcgaa cttaaaatct ccaatactta taacaccaaa gacatatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcaa ataaggtata taaaataggt
     1621 ggaggattga cttctaaggt aatgagctct atagcatcat cattatctaa acacaataca
     1681 actccaacag aaccaggaaa tagtgggggc aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttagagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2461 atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg agtattcatt
     2521 tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt gttttttgtg
     2581 gatttttatg tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt
     2641 tttttatttt ttgagtttta ta
//
LOCUS       DAMJZE010000287         2560 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMJZE010000287 DAMJZE010000000
VERSION     DAMJZE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.293"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(226..1476)
                     /locus_tag="PC541_003731"
     CDS             complement(226..1476)
                     /locus_tag="PC541_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2841146.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1508..2449)
                     /locus_tag="PC541_003732"
     CDS             complement(1508..2449)
                     /locus_tag="PC541_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2841147.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          769 a          494 c          240 g         1057 t
ORIGIN      
        1 aatacacatt taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat
       61 tatagcatag aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac
      121 tattattaag aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa
      181 acaagggcta aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc
      241 atgtaagaat agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat
      301 cttatttgca tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc
      361 tactttcata tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat
      421 aaaatcaatt cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata
      481 gtcttgtaat atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt
      541 atttgctttc attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc
      601 aggtttatat ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt
      661 tataaattct tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac
      721 cattaaattc tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt
      781 ataatttgga tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg
      841 atgaccatct gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat
      901 tatatcatca ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc
      961 atataaaggt tcattattat ccataaaatc attgattata tcatctatca ccctttgatg
     1021 tttgtaagtt acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct
     1081 atcaatttcc ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc
     1141 atcaactgat atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata
     1201 tatatatata cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa
     1261 attttctcgc gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc
     1321 aatttctgat ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc
     1381 ttctttacta ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt
     1441 tggtctaatc gccttaaatg gtttaatcgt tgccattact aatttcctcc taattataca
     1501 aaatatttta taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca
     1561 catttactat ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag
     1621 gtgtaggaga aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat
     1681 aaacatctat agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt
     1741 catcaactaa accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta
     1801 tttctttttc agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga
     1861 aatctgcatt ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg
     1921 cctcacccat tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa
     1981 cttcttttgc aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac
     2041 ctttatactt tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga
     2101 atcttgcaag aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat
     2161 ttggagtatt cataactttt atgccattag cttgagcata tttaacatct atattatcca
     2221 gacctactcc acctcttact atcaacttca actttttacc ctctaatgct ttatctatta
     2281 catctttagt aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat
     2341 ctcctaaaac atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt
     2401 ttctcgctgc ttctttctct attccatcag taactaatat attatacata acaaactcct
     2461 ccaattttta ttcacttaat ttttccaatt tttatttttt aaagtatttt catcaataaa
     2521 aaactttact taatttaact ttctgtttat ataattttca
//
LOCUS       DAMJZE010000288         2554 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMJZE010000288 DAMJZE010000000
VERSION     DAMJZE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.294"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..1133
                     /locus_tag="PC541_003733"
     CDS             <1..1133
                     /locus_tag="PC541_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841148.1"
                     /translation="LLNNEDNLKIKTIKGDSNELDHININFGYALNGYINSYYTINGS
                     NKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVGFEMNEESKDINFVIDEKKEDLEK
                     GVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDDYYAKILASKRYKKYLYVSVLYAT
                     SDSSENNKEYSSTIDIIKINPENKKIKTIKSINLSNYFSEKYQNIENIGVSYASKYED
                     KMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESIMELKTDSKMALAKAKLEANKLYM
                     VMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKIVAYTSRL
                     NSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNSMLEFEIK"
     gene            1142..1954
                     /locus_tag="PC541_003734"
     CDS             1142..1954
                     /locus_tag="PC541_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841149.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            1965..>2554
                     /locus_tag="PC541_003735"
     CDS             1965..>2554
                     /locus_tag="PC541_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841150.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICIL"
BASE COUNT         1098 a          202 c          383 g          871 t
ORIGIN      
        1 gtttattaaa taatgaagat aatttaaaga taaaaactat aaaaggtgac tctaatgaat
       61 tagaccatat aaacataaat tttggatatg ctttgaatgg atatataaat tcatattata
      121 caataaatgg aagcaacaaa accaaaaaga ctgaattaat aaaactacaa aaggatagta
      181 agaacacaaa taatttaatt gatgtatcta gtggttcaga tttaattgct catgttggat
      241 ttgaaatgaa tgaggagtca aaagatataa actttgtgat agatgagaaa aaagaagatt
      301 tagaaaaagg tgtatttcca tcagaaggta gtgataaaga aattgaatat gaaaaattag
      361 atatgaataa ttttacaagg acaagatata aagtagataa aagtacagat gattattatg
      421 caaagatact agcatcaaaa agatataaaa aatacttata tgtatctgta ctttatgcta
      481 caagtgattc atcagaaaat aacaaagaat attcaagtac tatagatata ataaaaatta
      541 atccagaaaa taaaaaaatc aagactataa aaagtataaa tttaagtaat tatttttccg
      601 aaaaatacca aaacatagag aacataggtg taagttatgc tagcaaatat gaggataaga
      661 tgtatatttt agtaaatgta ctaaatacaa acataggtga taatagtaat tctaaatttc
      721 atttacttga gtataatctt gaagaaaata aggaaagcat aatggaatta aaaactgata
      781 gcaaaatggc tttagcaaaa gcaaagttag aagctaataa attatatatg gtaatgtcga
      841 atgattatga taatagttca attacagagt taaatttact tacctataat atattagata
      901 aaagcttaga tgaagataaa tatgttttag ataatagaga aaaaagagga attagaagta
      961 ttgaaattga ttcaaataaa atcgttgcat atacatcaag gctaaactct atattatctt
     1021 gggatgataa atttatgcta tatataataa ataaaaattc taaggaaatt gtatatgaag
     1081 gagaattaca atttaacagt tcaaatagta tgttagaatt tgaaataaag taggtgagtt
     1141 gatgaaagat ttaataagat tattcgaatt tgaattaaaa agaaatataa agaattatat
     1201 atttataata atttgttgtt gttcttttgt aattttaaac atagtaaaaa atttaaatga
     1261 ctataattac ataatagaaa atgcagttaa aagtgaacag ttaacaaaaa tcggaaatac
     1321 gatagaaacg gtaaatgtta caggtttctc aagctttaga aatataatgg gttctacaga
     1381 atcgtggttt atgtttggta taattgcatg tatttgctat gcttttttta tttggtatag
     1441 agactttaat ggtagaagta aatctatata tacacttata atgctaccta aaaatagaat
     1501 caatatttat atatctaaat tattaaatat attatttcta gtgtatagct atacagtagt
     1561 acttacaata tctttgttta ttgcaagtaa gttgttgcct agacatatgc ttggtaatgt
     1621 cacaaactat ggttttgtac aagaaacaat atatgagtta aaaatgttac ttccatatag
     1681 ctttgaaata ctatttgtag agtatatatt tttattgata ggatttgtat ctgtagtatt
     1741 tacatcaatt cttataaata aatctattta taaagtaagc actttaataa gttttctatt
     1801 tttagttata gaaattttag tgtttataat ttctatagaa tttattatac cttacgaata
     1861 cagtgatgta tttactgttc tatattcaag tataaacata tttgtatgta gtcttatatc
     1921 taataaactt ttgaagcaaa agattgactt ttaggaggga tattatgaaa gcaaaaaata
     1981 taggaaaata tacaatatgt tttataatag tatgtttttt aatgattact atgaccttaa
     2041 tattgagtta ttttaataag gataaactat ctgtaaaaac tttaaaaggc aatgtaaaag
     2101 atatagggag agtaagtgta gtatatacac ctaatataag caaattttct tatgaggagg
     2161 taattataag taaaaatgga gtaagaaata atacaaaccc aaaatcaaga attagaaatg
     2221 attatttaaa tattgacaaa aaatacagtg actttgttaa tgataaagga aaagaattta
     2281 tatataaaga tgatttgaat ataggagaag cgtatacctc tgttaagtct gatttagtac
     2341 aaggaatgaa tgctttaaat gattgtataa aggtttcaaa taaaaattta aaaagtgggg
     2401 tagaagaaaa ttttgatatt aatttaaata agtctgattt taaagaaaat agttggaaaa
     2461 ccttatttaa caaaatatat aaaggtaatt tatatatggt aattagtgaa actaatattt
     2521 caggttctaa agtcaataat atttgtatat taaa
//
LOCUS       DAMJZE010000289         2473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMJZE010000289 DAMJZE010000000
VERSION     DAMJZE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.296"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(397..1131)
                     /gene="erm(C)"
                     /locus_tag="PC541_003736"
     CDS             complement(397..1131)
                     /gene="erm(C)"
                     /locus_tag="PC541_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="HDF2841151.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
                     PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
     gene            complement(1192..1251)
                     /gene="ermCL"
                     /locus_tag="PC541_003737"
     CDS             complement(1192..1251)
                     /gene="ermCL"
                     /locus_tag="PC541_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDF2841152.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
     gene            complement(1946..2422)
                     /locus_tag="PC541_003738"
     CDS             complement(1946..2422)
                     /locus_tag="PC541_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2841153.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
                     QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
                     GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
BASE COUNT          751 a          387 c          377 g          958 t
ORIGIN      
        1 aaaaaaaaaa gaacatttgt taaaagaaat tagaacaaaa tgagtgaaaa attagaacaa
       61 acaaattcct tataaacctt atcatctcaa cctatattaa gattttacct agttgaatct
      121 tcttttctat ataaagcgtc ggagcatatc agggggttat ctaacgtaaa tgctaccctt
      181 cggctcgctt tcgctcggca ttgacgtcag atactgcacc ccctgaaccc ccatgctcca
      241 acagcaaaaa ggaaactttt tgctgctttt ccgacgctta ttcgcttcgc tcatatttat
      301 atagaaaaga agcgaatgcg caaaagacat aatcgattca caaaaaatag gtacacgaaa
      361 aacaagttaa gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct
      421 attgaaaaga gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg
      481 ttttaaggaa ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca
      541 tttcataacg aaataattat acttctgttt atctttgtgt gatattcttg atttttttct
      601 atttaatctg ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac
      661 catacttaat atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt
      721 atttaataat cttttagcaa acccgtattc cacgattaaa taaatctcat cagctatact
      781 atcaaaaaca attttgcgta ttatatctgt acttatgtta taaggtatat taccaaatat
      841 tttataggat tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa
      901 attatcgtga tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat
      961 ggcagttacg aaattacacc tctgtactaa ttcaagggta aaatggcctt ttcctgagcc
     1021 gatttcaaag atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt
     1081 atgttttgaa gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct
     1141 ctttaatttg gttataatga attttgctta ttaacgattc attataacca cttatttttt
     1201 gtttggttga taatgaactg tgctgattac aaaaatacta aaaatgccca tattttttcc
     1261 tccttataaa attagtataa ttatagcacg agctctgata aatatgaaca tgatgagtga
     1321 tcgttaaatt tatactgcaa tctgatgcga ttattgaata aaagatatga gagatttatc
     1381 tagtttcttt ttttacaaga aaaaagaaag ttcttaaagg ttttatagtt ttggtcgtag
     1441 agcacacggt ttaacgactt aattacgaat taaataagtc tagtgtgtta gactttaatg
     1501 ttttttaaag gcattagtgc acttaagcgt cagagcatgg ctttatgccg agaaaactat
     1561 tggttggaat ggcgtgtgtg ttagccaaag ctttggcgag ttggttgggg gtttcatggg
     1621 attaatccca tgaaagtacc aactcaacaa cacactaacg cctgttggtt ccaaccaata
     1681 ggaaattgga ataagcaatt agtataatga gagtataatg ttggtataac gttagtataa
     1741 tgatgctttt tttcattata ttttttatgt actttaaacc tgcacgctta tgcgaattag
     1801 aaaaagctta atcgcatttc atagattgac ctcccaataa ctacgtggtg ttattgggag
     1861 gtcaatctat ttcatttgcg ttaagctttt gatctacatt aagcgtgcag gttttttgtc
     1921 tatttgtaga cgattttatg ttatactagt ccttgaaaga ataataatca gataatgcat
     1981 tttcttgttt ttcatttgcc tcttgctcaa agttcccaaa ttcgagtaag aggtattttt
     2041 gtttttggtc gtcgcctctc attagtagtt cagggtttaa cattaatact ccagtttttc
     2101 tttttataat atttccttct tctaagattt taagtgttgt tattactgtt tgtagacttg
     2161 ttcctgtagc ttttgctatt tctcttgttg tagctatcat tgtattgtta cttaagtgga
     2221 cattatctag gatatagtta acgattttaa gtttttttcc gccaatcata tctaacatac
     2281 ttattaattg cactatatat gcctttacga agttaccaga cgtttgttta cggtataact
     2341 tgtctacctc tatgacttct ccactttctt cgtctatgag cctctgagag cctttataga
     2401 ctgttccata tctttctttc atctttttct cactccttat tttaaactat tctaactata
     2461 tcataactgt tct
//
LOCUS       DAMJZE010000290         2473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMJZE010000290 DAMJZE010000000
VERSION     DAMJZE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.297"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            59..793
                     /locus_tag="PC541_003739"
     CDS             59..793
                     /locus_tag="PC541_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2841154.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(936..1427)
                     /locus_tag="PC541_003740"
     CDS             complement(936..1427)
                     /locus_tag="PC541_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2841155.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1776..2159
                     /locus_tag="PC541_003741"
     CDS             1776..2159
                     /locus_tag="PC541_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841156.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
BASE COUNT          962 a          277 c          363 g          871 t
ORIGIN      
        1 atattttgta tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat
       61 gaaacaaaat aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc
      121 tacaaatgga ttagcaggtg ctggagagtg gcatgtttta aaaaaaatgt tacctgattt
      181 caaaggaaaa agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt
      241 tgagaatgga gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc
      301 tagaaacaaa acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa
      361 ttttccaaaa gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc
      421 atttgaagat atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt
      481 ttctgttgaa catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga
      541 tggcactaat ctacactggc cagtagatgg atattttgaa gaaggagtac gtaagtcaaa
      601 ttttctagga gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt
      661 aatgaaaaca ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt
      721 agatacaatt cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc
      781 aaaaaaaaga taagtaaata taaataatca ttattgtaga aaaaaagttg tagaaaataa
      841 ttacactaat ggagatgatt ataccagtgg tattaattac accagtagta aggaggcaaa
      901 agcattcgtt gcttttgcca ttttttaagt tgcaattata cttctacgta gtatatttcg
      961 tttccatttt cttctataag ctttttcata tcttcaaatt ttaaaaatac agtagcagta
     1021 ttatcatttg gatgtatacc tattttatcc atttttaata aatctttatc aaatacaact
     1081 tttacaacag cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt
     1141 ttcaagtatt tttgaagtct ttcttccgat gcaaaactaa gaggtttact atcaatttgt
     1201 tgacgaatat cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct
     1261 tttcctttag catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt
     1321 aattcttcca tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg
     1381 cttaactcat ctaatttttt atatacttcc tgttgttgat tgttcatgat ttcctcccat
     1441 tagaaattta ttttatatat aaataatatt atcacagaaa actttaaaag aaaacgtttt
     1501 atataatata gacaatagat tatattttta taaagaagca agaatactca ataaaactta
     1561 aagaaattaa acaatttcta aaaatacctt gaaaatagaa ccttatttgc catttataaa
     1621 ttatataaaa agggattata taaagtcata tatgaaaaaa ctatattata ctatcttttc
     1681 tttttggaat aaactatact ttaaatatag atatttaatt taaataaatt ttacagtatt
     1741 aatagtaaat tatcttagaa aggaatgtgc tttatatgtc tacagacaaa gttgttggaa
     1801 tcatagatga agaaacggca gaacttgcag gaattgaata tactggtaaa atttatgctt
     1861 cttctggtgt tataaaacat ataaagaaaa aacatagatg tcaactgagt aaggatattt
     1921 ttaatgatat aatagacact ataaaaatgg tattaaagtc acctgaatat ataggaagtc
     1981 atcctaaaaa acctggaaaa agtgtggagt ttataaaaaa aattgatgat tatattttag
     2041 tagctacaga attagataca aaaaaaggat atttatatgt atcttcaatg tatgctataa
     2101 aagaagcaaa attagaaagt agagtaaaca gtggaagagt aaaacaatat aatggttaac
     2161 ataatattat taattttccc taaaatattg aaaaatatta ttatataagt tataatatta
     2221 atatacacgt agatatttat tagttagatt tgaggttgga aaaggcaccc aatacgctac
     2281 tatagagtag ttaatagaga tgcaggatac gccgccctgc caaatctaat taaattaaaa
     2341 agagaggatt aattcctctc ttattgtttt tttgagatac taatttaatc tataatagaa
     2401 agattgaagg tcgtttatac aaaaaaattt tcagttatat acattaaact aattaaataa
     2461 gatatttata atc
//
LOCUS       DAMJZE010000291         2452 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMJZE010000291 DAMJZE010000000
VERSION     DAMJZE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.298"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..924
                     /locus_tag="PC541_003742"
     CDS             <1..924
                     /locus_tag="PC541_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2841157.1"
                     /translation="TKFDVVTLLDNKKVDTQIAKVITDLQDNDYITWKKDATLEASAG
                     LVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLFVEFTKRMRDKVGA
                     KFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGCDINKSNTNKKYDG
                     EFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTDDKNDDFSSNQSVR
                     VLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENIRAIEDFKTDDVSV
                     ELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            941..1411
                     /locus_tag="PC541_003743"
     CDS             941..1411
                     /locus_tag="PC541_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2841158.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            1483..1923
                     /locus_tag="PC541_003744"
     CDS             1483..1923
                     /locus_tag="PC541_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2841159.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            2264..2425
                     /locus_tag="PC541_003745"
     CDS             2264..2425
                     /locus_tag="PC541_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841160.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
BASE COUNT          980 a          241 c          507 g          724 t
ORIGIN      
        1 actaaatttg atgttgtaac acttttagat aataagaagg tagatactca aatagcaaag
       61 gttattacag acttacaaga caatgactat atcacttgga agaaggatgc aacactagaa
      121 gcaagtgcag gacttgtatt tactggtgga actaatggcg aagctgtgac aggagcagag
      181 taccaagctt tcttggataa aatagaaagc tatagcttta atgctttagg atgtttggct
      241 acaacaacag aaattaaaag tttatttgta gaatttacaa agagaatgag agataaggta
      301 ggagctaagt ttcaaacagt actatataag aaaagtgatg cagattatga aggtgtagtg
      361 tctgtagaaa ataagattaa agataaagat ttagttgaat ctagtttaat ttattgggcg
      421 gctggagcta tagcaggatg cgatataaat aaatctaata ctaataaaaa gtatgatggt
      481 gagtttgatg ttgatgttaa ttatacacaa atacaacttg aagaagcttt aaaaactggt
      541 aaatttatat tccacaaggt gggagatgaa gttcatgtgt tagaggatat aaatactttt
      601 gtatcattta cagatgataa aaatgacgat ttttcaagta accaaagtgt tagagtactt
      661 gaccaaattg ctaatgatat tgcaacttta tttaatgaaa agtatttagg taaagttccg
      721 aatgataagg caggaagaat aagtttctgg aatgatgttg ttaaacacca taaagaatta
      781 gagaatataa gggcaataga agattttaaa actgatgatg ttagtgtaga gcttggaaat
      841 gataagaaaa ctgtcatagt atctgatgct gttaaggtta taaatgctat gagtaagctt
      901 tatatgacag tttcagttag ttagagaggg gagtgataat atggctcaaa caataaatgc
      961 taaagataca gttagtgcaa agaaagctga atgttttata actatagaag gcaaaagata
     1021 taattttatg caagctatag atttagaggc taaaatggaa aaaaataaaa gtgaagttcc
     1081 aattctagga agaacaacaa agggaaataa aacaactggg agtacaaata ctggaagtgc
     1141 aacatttcat tataatactt ctatttttag agaattactt tacagatata aagaaactgg
     1201 tgaggatatt tattttgaca tacaagttac aaatgaagac cctacatctg ctgtaggaag
     1261 acagacagta gtacttaaag attgtaatat ggacagtgga ataattacta aatttgatgc
     1321 tgatggtgag tatttagatg aagatatgga tttcactttt gaggattggg aattagtaga
     1381 aaaatttaat ttattggcag gaatggagta aaatacacat ttataaatta tatatgtgta
     1441 ttttttatat gaaaaattaa aataaaagga gattagaata atatgagtaa tttaagtgct
     1501 tttttaagtc aaaatgcaat aaaggttgat aatgtaaaat atgtagcgag taacagattt
     1561 ttagataaag aagggaaacc agttgaatgg gaattaaaag ttttatcatc tgaagaagac
     1621 gaagcactaa gaagaaagtg tactaaaaga gtaaaagtga ttggtaacaa tggtaagcat
     1681 actggacaat atacaagtga aattgactac aatagttatg tagctgaatt atgtgtagca
     1741 tctacagtat ttccagattt aaaggatgcc gaactccaaa atagttatgg agtaatggga
     1801 gaagctcagt tattaaagac aatgcttaca gcaggtgagt atgtcaatta tacagtaaaa
     1861 gtgaatgaag tcaatggatt tgatacatct tttgaggata aagtagaaga agcaaaaaac
     1921 taatcagagg tggcgatttt gatgctagca tcactcatta ttgtattcaa aaattaaagt
     1981 ggaagccaag tgaatatatg aatttagaag ttaatgagag agcgttagca gccgcctcaa
     2041 tacttataaa gatagaagat gaagaggaag caatgaaaga agctgaaaga gagagaaaga
     2101 ggggacgaag aagatagcaa aataaaaaaa taaatataga ataggtaaaa tatgtaataa
     2161 ttatatgtta taatattttt agcaagaaga tgtaatctac aatttataga gtggagttca
     2221 tactgagata aaacctactt cctaatgaaa ggaggtggga agtatgaata actttttact
     2281 taatgtaata gctggcgtta ttgctagttt aatattttgc ttaatttgta aagtatttct
     2341 aaaagtaaaa agccactcaa ctcgtggcaa gagtaaaagt ggctgggaat ttgattttaa
     2401 aatcaagttc cataagttca aatagattca tttaattatg aacttcactc ta
//
LOCUS       DAMJZE010000292         2430 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMJZE010000292 DAMJZE010000000
VERSION     DAMJZE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.299"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            329..2224
                     /locus_tag="PC541_003746"
     CDS             329..2224
                     /locus_tag="PC541_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2841161.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1061 a          244 c          448 g          677 t
ORIGIN      
        1 agatagatat taatttagtg atatatattt aagtaataag agtaaagcaa aaattagtgc
       61 atagtagtat ataaaaatgg agtttgaata cttttgttag tatatgattg tttagtagaa
      121 aatcactata aaataggatt tctatctttg agcataatta tagagagcta agcaggggga
      181 aggggttcaa gaagatatta ttagtgtttg tgttagtaaa ttatgtaaac acataaaata
      241 tattgaaaaa taatattata tataatatta tttatgtgtg ggaaatacga atataaaaaa
      301 taaaaaataa tattcggagg acataaatat gttaagcaac aaaaagagaa gtatggcaat
      361 agtaatggca ggtgctacag ttatgagtgc agcagcacca atattcgcag acaatactgt
      421 gacagaaaat gttgataaaa attacacagt aagtgcgaaa gattctgcta aactaatcga
      481 agaggttaga aaagcattag aagttaagtt tgaagataca aaagcaggag caaatgtaaa
      541 tgatagagtt tacgatataa aagtagacaa tgtaaactta actaatgcaa ctcaattaca
      601 aaataagata aactctttaa cagaagggca aagtttaaaa gttactattc aagataaagg
      661 acatcaagtg ttaggtggaa aagtagttga ctataaaatt gaaaactaca aaactgctca
      721 agaaatagta gatgcagtta atgcttacaa tgcaacttta gcagaagatt ctgataacaa
      781 gttaacagca actataaaat ctacaaatac agttgaagta aaaagagcaa aagattcagc
      841 aaatgttatt acattaaatg taggagacca acatttagat ttctcaaaag tgataacatc
      901 agaagaaggt acttttgaag gttatgaaaa gagttatagt gacatagatt caaaagaatt
      961 acatacgata acagttaaaa acgcagattt acaagacata tcagcagaag aattatttga
     1021 tggaattaga ttaactacat taggaagaga aatagttaat aaagttaaaa atggatatgc
     1081 tttaacattt gaaaatgaag caatccttac tcaagaacaa gaagatagtg atgataaaga
     1141 taaaccagaa aaatcaagct ttgatatagt tttaagcaag gcaaatgaaa agccagaaac
     1201 aataagtgtt tcaagtaaaa accataagtt agttagagac ttacacaaag tattaactga
     1261 tgtaaaaggt ggaaaagaat taaaagtaga agttttatct ggtgattcaa gatttacaac
     1321 agcagtagaa gttagtaaag agagattcaa agatggagaa gcagaagcta taattttagt
     1381 tggagaagat gctatagttg atggattagc atcagcacca cttgcctctc aaaaaaatgc
     1441 accaatatta ttatctaaaa aagattcact accatcagaa atagaagctg aaatattaag
     1501 agtacttgga agtaatctat cttctaagaa aatatatata gtaggtggag aatctaaagt
     1561 atcaaaagaa actgaagaaa aactttctaa attaggtgta agtaaagttg agagagtttc
     1621 tggagaagat agatttgaaa cttctttaga aatagcaaaa caattaaaag atacatttaa
     1681 gactgctttt gtagtaggtg gaaatggaga agctgatgct atgagtatat cagctagagc
     1741 tgctcaattt ggtgctccaa taatagttac aggtaacgaa ttagatgcaa atgctgaaaa
     1801 attattaaaa ggaaaagaat tagaaatagt gggtggagaa aattctgtat caaaagaagt
     1861 tgaagacaaa ttagttgata tagatttaaa taataaagtt gaaagattag ctggagaaaa
     1921 tagaaaagat actaatgcta aagtaatcaa taaatactat gcaggtgcaa ctaaagcata
     1981 tgtagcaaaa gatggttatg taggtggaaa tggacaatta gttgatgcac ttacagcagc
     2041 accacttgca gctagttcaa aggctccaat agtattaact acagaagaac tttctaaatc
     2101 acaagaagaa gtagttgagt taagacttaa gaatgctact aagttagtac aaataggtga
     2161 aggaatagct aaaaacgcta ttgaaaaaat agcagaaaaa ataaacttat ttactaagaa
     2221 ctaataagat ataggaatta atattaaaag tttgtttaga taatatatga ttatacaatg
     2281 cgttaatatg taagacatag tgtagatatt ataaaatata caaataacaa gataaaataa
     2341 taaaaataag gtgtctcatt tagaattaga ttgagatacc ttattttatt ttttgattat
     2401 aatgttgaga aattcatatt gcaaaaatta
//
LOCUS       DAMJZE010000293         2373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMJZE010000293 DAMJZE010000000
VERSION     DAMJZE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.300"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            204..2276
                     /locus_tag="PC541_003747"
     CDS             204..2276
                     /locus_tag="PC541_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2841162.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT          873 a          256 c          455 g          789 t
ORIGIN      
        1 aagattgtta ctgataatgc aggcaagact tatgtttata aaaaaattat aatagtttat
       61 ttttaattct gaaattaggt taatataggt ttgtttatat taacataagt ttaattttag
      121 aattaagtat actaatgtaa ctttttataa acttaagtct tttttattta aaaaaatata
      181 aaataataag gagagaaaag taaatgaaaa aagtatttgg tgttttacaa aaagtaggaa
      241 aatctctaat gctaccagtt gcattacttc ctgcagcagg tatattactt ggcgtgagta
      301 atgctttagc aggagacgca ttgttggctc attttccagc tttggcgaat cctacatttc
      361 aacttataat aagtataatg gaaagttcag gtcaaattat atttaataac ttaccactta
      421 tatttgcagt tggggtagct gttggtttaa ctgaaggaga aggtgttgca gcattggcag
      481 ccatagtagg ttttttaatt ctcaatacat ctatgggtgt tatggcaggt gttacagaag
      541 atatggtaga caattctatg tatgcaaata ttgttggaat accaacaatt caaacagggg
      601 tatttggtgg tattattata gggatagtgg ctgcttatct ttataaaaag ttttataaaa
      661 ttgaattacc tcagtatcta ggtttttttg caggtaaaag atttgtacca atcgttactt
      721 cattagttgc tatagcaata ggaattatat tgtcttttat ttggcctcca atacaagagg
      781 gattacttag tttctcaaaa aatgttatag aagcaaatca aacactagca gcatttatgg
      841 aaggttcttt ggaaagacta ttaataccat ttggtttgca tcatgttttc cataatccat
      901 tttggtatca atttggagaa tatgtaaata aagctggtga gcttgtaatg ggtgaccaaa
      961 agatattctt tgctcaactt aaagatggag taaatttcac agcaggtact tttatgacag
     1021 gtgcatttcc atttatgatg tttggtcttc cagcagcagc tcttgctatg gtgcatgaag
     1081 caaagccaga aaagaaaaaa tttgtagcag gtataatggc atcagcagca ttaacctctt
     1141 ttttaacagg aataaccgag ccaatagagt ttgcgttttt atttgttgca ccagtattat
     1201 ttgcaataca ttgtttattt tcaggtttat catacgcaat tatgcaatta ttaggagtta
     1261 aaattggatt cacattttca ggaggattaa tagatttctt attatttggt gttcttccta
     1321 atagaactcc atggtacttg gctatcttgg taggtattgg ttttgcagta ttatactata
     1381 gtgtatttag atttatgata agaaaattta atttaaagac accaggaaga gaagatgagt
     1441 cagaaactaa cgctgaagtc attgaaatgt ctaagaatga attagcatca gagattttga
     1501 ttgctttagg aaataaagac aacttaaaga gtctagatgc gtgtataaca agacttagat
     1561 tggttgtaaa tgatataaat aaagtagata aagaaaaatt gaaatcactt ggagcagcag
     1621 gtgttatggt tattggcaac aatgttcaag ctatatttgg accaaaatca gattcaataa
     1681 agtctgatat aaaagatata atagagggaa aaacaaaggt tgaaagtact gttactaaaa
     1741 atgttgataa aaataaagtg aaaaaaaata tggaagttgc aaatatggaa aatactttag
     1801 tagaaaagat tgattcatta acaaatggag agttattaga tatagaagaa gtgccagatt
     1861 ctgtattttc aagtaaactg atgggggatg gatttgcaat taaatctagt gatggattga
     1921 tttattcacc agttgatgga actattggag ttatatttcc aactaagcat gcaataataa
     1981 taaaatccca aaaaagtgga gtagaaatac ttatacattt agggatagaa actgtaaact
     2041 tagaaggaaa tggatttgaa gtatttgtga atgtaggaga tgaagtaaaa gcgggagaaa
     2101 aattagttaa aatggactta gaatatattg aaaaaaatgg tctatcaaca ataagtcctg
     2161 tagtatttac gaatttagaa caaaatcaga aattaagcat taaaaaagga gtaaccacag
     2221 caaaagagga gaatagagta agtatattaa gagatgatat tgaaaaaata ggatagctat
     2281 atgatagtgt aactaaaagt tatgtgagat ttaataaata tataaatagg ttcagttaac
     2341 atgcaattta tttaatatat attttaagta ata
//
LOCUS       DAMJZE010000294         2335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMJZE010000294 DAMJZE010000000
VERSION     DAMJZE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.301"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            49..495
                     /locus_tag="PC541_003748"
     CDS             49..495
                     /locus_tag="PC541_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2841163.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            497..1591
                     /locus_tag="PC541_003749"
     CDS             497..1591
                     /locus_tag="PC541_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2841164.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1679..2209
                     /locus_tag="PC541_003750"
     CDS             1679..2209
                     /locus_tag="PC541_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2841165.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
BASE COUNT          971 a          232 c          415 g          717 t
ORIGIN      
        1 atagttgata tagtcaaaca tattataatt atagaaggga aatttaagat ggattttaaa
       61 aatttacagt atgaaagttt gtcatatatt atttgtaact tgcaaaaaaa ctttaaactt
      121 tattgtgaaa aatgtttaaa accatataaa cttacgaatg gtctatattt ctatttaatt
      181 tatattaata aaaataggaa ttgttctttg aatgatgttt ctacagaatt tgaagttgat
      241 aaagctcata caacaagaac tatatctagg ttagaacaag atggatacat agaaaaaatc
      301 caaaacccta atgatagtag agcttttcag ttgagagtaa cagataaggg tgaggaagta
      361 ttaggggata taaaaaacat attttcaaag tgggataatc acataaaaaa agagttctcg
      421 gatacagaat ataaagaact ggtaaagaat ttacatgtgg taaaggatat aaaaacagct
      481 gtggaggaag aataggatgt attgtaattt attaaaacca attaaaattg gaaatttgga
      541 attaaaaaat agagtatctt ttgcacctac aagcatgggt cttaaattgg aagaaaaaat
      601 taaaaagttt tctgatattg ctaagtcagg agtagctctg attactctag gagatgtatc
      661 tattagacct agttttcata aggtagctat aagcctatcg gatgaagatg gagtcatgaa
      721 atacaagaaa attgtagatg aaatacacaa ttctggtgca aaggtatcag cacaactatt
      781 ttgttcagat tatgatgtta atttaattaa agatactatg aatatgggaa ttacttcaca
      841 tgatgagata aaaaaaataa tgaatgacgg agtaaaagat tacataacaa atatgcccaa
      901 agaagaaatt aaaaatatta taaatttatt taaagtaaca gcattgaatg caaaaaaagc
      961 aggttttgac atgattcaaa ttcatggtga taggcttgta ggaagttttt catcaagtat
     1021 atttaataat agaaatgatg aatatggtag aacgtgtgat aatagaagta gatttgcaag
     1081 cgaaataatt agtagtatcc gagatgaagt taaggacatt ccaatagact ataaatttgc
     1141 aataagacaa gaaaatcctc attatggaaa tgcaggagtt ttactttcgg aagttgagta
     1201 ttttgttaaa aaatttgaat ccctaggtgt aaacagtttt catgtaacac ttgcaaatca
     1261 ttcaaaatta gaagatacta taccaaccaa taatcatcct tactttaaag aagaaggctg
     1321 ttttttatat ttagctgatg aagtaaagaa acacacaaat cttccagtat gtggggttgg
     1381 aaaattgtct agcccagatt ttatagaaag tattatttca aataatagag tagatatggt
     1441 ttctatgtca agacaattac tagcagatag taattggtta cagaaagtaa aagatggaag
     1501 agtagatgag attaaaaagt gctgttattg taataagaag tgtgcagatg ctttacaaac
     1561 acgctctcag tttggatgta tactagatta gaatatcctt ataaaaataa ataatttgtt
     1621 aaggttaata tatttttggt gatttagata aatatttaga taaagaggag gagacatcat
     1681 gaaaagttta gttttatatt caagtttaac tggaaatact aaaaagattg cttatactat
     1741 atatgatgaa attcaagaag aaaaagacat taaagatgta aatgaattag ttgattatgg
     1801 tattgactat gaaaattatg atatagtatt cttaggctat tgggtggaca agggaatttg
     1861 tgataaaaat tcaaaacaag tgcttgaaaa tattcacaat aaaaagatag cactatttgg
     1921 aacaatggga gcaagtaaaa aaggaagtta tggagctagt ataatagaaa agatagaatc
     1981 aattatccca aaagataatg aaatactagg ttcattcata tgtcaaggta agatagcaga
     2041 aggattgaaa gcaaaatata aagagatgtt aaaactttct cctgacaacg aacatattcg
     2101 ccaacaatta aataaccatg aggaatctca aagtcatcca gatgaacaag aaatatatga
     2161 agccagtatg tttgcaaaaa atatgatgat taaagctagt attgtgtagt agttaaacaa
     2221 taagttcata taagatatag aataaataat ttagtaaaaa agtatagcaa ataatttgct
     2281 atgctttttt tatgaaatca aaaaaataat aaaaatactt gactgtaaac cttgt
//
LOCUS       DAMJZE010000295         2335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMJZE010000295 DAMJZE010000000
VERSION     DAMJZE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.302"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(164..2257)
                     /locus_tag="PC541_003751"
     CDS             complement(164..2257)
                     /locus_tag="PC541_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2841166.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT          778 a          394 c          233 g          930 t
ORIGIN      
        1 ttatcccttt ttaaaatact ttaatcatat actattccca atttaacatc cattgaaatg
       61 ctattttctt atattgcaat ttataattga tatatcttca atataataat ttaatgtatt
      121 tttgatttta aagcttttca gtgatattat tgataaatta gaattaatta tctagcttta
      181 aaaggtcaaa gatttctgtt gctgtctgcg gttttccaaa taaatatcct tgtatgtaat
      241 ctggctttat ttgtttaact aaatcatact cttcttgtgt ttctacacct tctaaacata
      301 cttttattcc tacatcatga caaattgcaa caataaaatg tataaatgta gcatcaaact
      361 tactctttaa tatatcttta acaaatactc tatcaatttt cactatatct attggagaaa
      421 attttaaaac ttctagagac gagtatccag taccaaaatc atccatagca atataaattc
      481 ctaaatcttg taaagcctta aatttaaact gtaaaatact catattttgt actgtatgac
      541 tttctgtcaa ttctaaaaca agattttgat aaggaaaatt ttcatcatct ataattttta
      601 ctatatcttc aataaatgtg tcttctaata tttgaacagc tgaaacgttt acactcactt
      661 taaaaaaagg atagtaatca atccatttcc taaaagtacg taatgccatt cgaagcaccc
      721 aagcccctac aatatttatc atatcatttt cttctaatat tggaataaat tccaaaggag
      781 aaattgcttt gcatttgtca ttagtccacc ttaaaagaac ttctacacct attattttat
      841 gactttctgt atctacttgt ggctggaatc gaagactaaa ccccctaaaa tcatcattaa
      901 ttgatgcctt taaatcccgc atcatttcta atgaatacat tttatttttt aatatttctt
      961 gggagaaaaa aacaagcctg tttttaccat gctctttcgc atattgtaaa gagtaactag
     1021 cacacttata aagttctttt actgtatctc catgttttgg atatattaca caaccagcag
     1081 aaattgttat atttaatcca tattttctcc atagttgaag gtgtataatc atattctgta
     1141 tttgattata tagagttaga atttcatttt cttcaacatt atcaacaaga atgcccattt
     1201 cgtccccatc aagtttataa agttcagcat tatctggtaa aatagattgt acagactgtg
     1261 ctagtgtttt tattatatta tctccaaaat ccctatcata catctcatta acatttttaa
     1321 aatcgtcaat atctaataat actatgcata atttttcaat cataggattt gaaattttat
     1381 cttcaaatac actcataaat tcatgataat tcagtaactg tgtcaatgaa tcaatcttat
     1441 tttgtttacc taattgcgtc attattcctg caaaaatact tggctctcca aattcatcac
     1501 gcattagttg acctctacat cttaaccata tatattctcc actacgattt ttagccctaa
     1561 attccactgt atgataatcc atctggtttt tacctatttc agtgtttgac ttataaaaac
     1621 gattccagtc ttctggatgt acaatttttt tccagtaaac aagcggattt ttaacaattt
     1681 ctcctggtaa gtcaaataat tcaacttgtg atggtgaata tcgaaatacg cctgttttca
     1741 tattgcaaat gtatatatat tcatctgtac ctctaatcaa agcatcgtaa agcaatgtag
     1801 aattataatc aaaattgtct cttgaaaaat taaaaactcc tttttcttct ttattatttg
     1861 atatttcatg aaattcatta atcttaattg gtttatttaa gcttgttgag tttgcaatat
     1921 ctattgattg atgcatatga acaactgtgc catcttgcca tgtaattaaa ctatcataat
     1981 tttcaaaaac acgatttaac ttattacatt tttcatacca ctttattaat actccttttt
     2041 tatcattctt taataattct aaaactttac aaaaactaca agtagaattt ttttcaggat
     2101 ataatacttg ccaacaaact tttccttctg gatctaaaat attatattct tctttcattt
     2161 ttttgttcat aaaaataatt tcattagtat gaatatttgt aacatatata ttaatctgta
     2221 gttgatttaa tataacttca aaattatgtt tgttcatata cttatctcct ccatagttac
     2281 tgctctttat aacaacattg ttaaaattat tctttttaga agatttactt ataac
//
LOCUS       DAMJZE010000296         2289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMJZE010000296 DAMJZE010000000
VERSION     DAMJZE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.303"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            448..1062
                     /locus_tag="PC541_003752"
     CDS             448..1062
                     /locus_tag="PC541_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2841167.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            1080..1442
                     /locus_tag="PC541_003753"
     CDS             1080..1442
                     /locus_tag="PC541_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841168.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1548..2045
                     /locus_tag="PC541_003754"
     CDS             1548..2045
                     /locus_tag="PC541_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2841169.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          874 a          255 c          351 g          809 t
ORIGIN      
        1 aatgtaagct ttgtcatact aacttcaaac aaattatgat gttttataaa ttatttgtaa
       61 atatcattat aattacttaa gttattcttt atcataattg cttaaattac tctttatcac
      121 ctaattatac accatcaaat aggattggta aaatattcat ctcatataag tattactata
      181 taataattta ttctattagt atactcaaat catgtaaaat ttatataact caattaattt
      241 tttaaataca actttataat ataaaactta taataaaagt aaatttatat gttaaatgag
      301 gttgattttt tattattttt tatttatgct agtactaaag acttgcaaac gttttttgac
      361 aagtctttat tttgtctaaa ttaaacaaac ttttttacaa ataaatacat aaaatttaaa
      421 ctaatctatt tattggagga gataaaattg gaagaagtaa ttgcaaaaat tattactata
      481 gaaaatggat tcaaagaaat tgaaaacatt gctaaaaaaa ttgtaaaaaa taatgactca
      541 aaaaagtgtt attatctatc aatctactta tataggtcag aatattatca agtaagagaa
      601 ttggctgtgt ttatacttgg ttttatatca gtaactattt ctgaagcttt gttattttta
      661 aaagataatg tgagtaaaga tgaaaactgg agagtacaag aaatactagc taaatcattt
      721 gattattatt gcagtgaagt aggatatgaa aaggctcttc ctgttataaa agaatggctt
      781 aattctgata gtccaaacat aaaaagggct gtgacagaag gtcttcgaat ttggactagt
      841 agagactatt ttaaatctaa tcctgacgtt gctatatcac tactttctag tttaaaagaa
      901 gatgatagtg aatacttgag aaagtctgta ggtaatgctc ttagagatat aagtaaaaaa
      961 tatccagatt tagtaaaaaa agaattagat tcatgggata tatcaaataa aaaagttgag
     1021 tttgtatata agcttgcttc aaaatgtatc ttggcaaagt aaaatggagg tgaacttatt
     1081 tgaaacttcg tgatgagtat acttgcccat tagaactaac acatgatatg ataaaaggca
     1141 aatggaaatc tattatatta tggagattac gcataggacc aacttcctta tcaaaacttg
     1201 aaaaggaaat caatggtatt aatcaaaaga tgttacttga acaacttaaa gaattaattg
     1261 aatttggtct tgtagataaa aagacatatg aaggttaccc acttaaagtt gaatactcac
     1321 ttacacctat tcgagggaaa caaatattgg atgctttggc tataatgcaa gatataggaa
     1381 ttagctttat gttggataat ggtatggaag atattcttaa ggaaaaaggt gttttaaaat
     1441 aaatagatac ccacaaaaaa gtaagtaatt taccattatt ttcaactggt atataattaa
     1501 attatagata tattctatta atttattata ataggaggta aattattatg aaagtaacta
     1561 gtacaggtat agtaaatggt gttatagaag ataaatacgg taaaagagga actcagttca
     1621 atgaaggtgg tatgcctaca tattcactac ctctaaagtt tgaagatgct ccaaagaata
     1681 cagtatcctt tgctgtattt ttagaagata aagactctgt tcctgtatgt gggtttgcat
     1741 ggatacattg gttagctgta aatataacaa aagatgagtt aaatgaaaat gaaagtgtaa
     1801 cagctactga ttttgtacaa gggactacta gttggcatgg caaactagga ggtcttgaca
     1861 gattagaggc cagtgtatat ggtggaatgg ctcctccaga cagacctcat gtctatgaaa
     1921 tacatgtatt tgcacttgat actatccttg accttgaaaa aggattctat atgaatgaat
     1981 ttttcaaagc tattgaaggg catgttttag atacatttac actaaaagga agttattcta
     2041 attaattagg ttgactatag ctaatcatta attaaatgct acatttaatt gatgattagc
     2101 ttaattgtta ttagaatatt ttctgcactt tatcttaata tatattttag ttgactggta
     2161 ttttaatttc tattagatta ttatcaattt atataaattg attttagctt aaaacttact
     2221 tatctatttc agtttctatt agaattatta ttagactcat taaaataaaa gcactaatat
     2281 aactaataa
//
LOCUS       DAMJZE010000297         2260 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMJZE010000297 DAMJZE010000000
VERSION     DAMJZE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.304"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            110..1561
                     /locus_tag="PC541_003755"
     CDS             110..1561
                     /locus_tag="PC541_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2841170.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1780..2241)
                     /locus_tag="PC541_003756"
     CDS             complement(1780..2241)
                     /locus_tag="PC541_003756"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2841171.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          765 a          265 c          395 g          835 t
ORIGIN      
        1 tttataaata ttataattaa attaatgaat ttttaacaag gaattattca tttttttgat
       61 tttaagattt attgagaaaa caaaactaaa taggggggta attaatataa tggatttttt
      121 caatggtatt ttaacgagta ttaacaatgc agtagtatat gtaaacaatt acttatggtc
      181 gtatatctta attataatgt tagttgtaat tggtatttac tttactatta aaacaaattt
      241 tgtacaattc agatatttta tagaaatgtt tagacttctg ggtgatggta cagcgaataa
      301 aaatgcaaaa aaggaaggaa aaatctcttc atttcaagca ttttgtataa gtacatcttc
      361 aagagttgga actggaaata tagctggaat agcaattgca gtggtagcag gaggacctgg
      421 tgctgtattt tggatgtggc ttatagcatt aattggttct gcatcaagct ttgttgagag
      481 tacattagct cagatttaca aagtgaaaga tggtcaagct tttagaggtg gaccagcgta
      541 ttatatggaa caaggtctaa ataaaaaatg gatgggtatt ttattctcaa tacttataac
      601 tatatgttat ggttttgtat ttaatgcagt tcaagcaaat actgtttcac ttgcatttaa
      661 taatgcattt ggaataagta aaatggctat gggaataggt cttggaattt taactgcaat
      721 agttattttt ggtggagtgc atagagttgc aaaagtttct gaaataatag ttcctgtatt
      781 tgcaggatta tatatattag ttgctttggc tatagttgta atgaatataa cagagatacc
      841 aagtgttata gcattgatag ttgaaagtgc atttgatttt aaaggaatgg cgattggtac
      901 atttatggga gttgtaatga ctggagtgaa gagaggtcta ttttctaatg aagcaggtat
      961 gggtagtgct cctaatgctg ctgcaacagc acatgtaact catcctgtaa aacaaggttt
     1021 aattcaatct ttaggtgttt ttacagatac tataataata tgtagttgta cagcattcat
     1081 ggtattatta tattcaggtt atagtagtag tggagcaact ggaattgaat tagctcaaga
     1141 agctttaact catcatatag gaccagttgg aaatatattt atagcagttt gtatattctt
     1201 gtttgcattt agttctatag ttggtaatta ctattatggt gaatcaaata tggagtttat
     1261 gagtggaagt aagactaaac ttaatgtatt tagagttttt gttgtaggta tggttttatt
     1321 tggttcactt actcaagtgg atgtagtttg gaatcttgca gacttattta tggcattgat
     1381 ggcgataata aacttaatag caatagcact tttaggaaaa tatgcattta ttgctttaaa
     1441 agattataca agtcaaaaaa aatcaggaat aaaagaccct gtgtttattg cagatgaaat
     1501 agaaggccta gaaaaggtat cagagtggca tagggaatta tctgaggaac atttaggata
     1561 aaaaatagta taaaattata aaaccatttt gaaagactag agtatgtata tactttatat
     1621 gaatataata ttgaattaat ttatattact ggaattttta attccagtaa tattttttta
     1681 ttatataatt tagctttatt aaaagttttt attattaaaa atagcttatt tatcgtaaag
     1741 tgaatgatat tgggttttaa ttattatgaa tgttagtatt taatttaaag acttaaataa
     1801 atttgccatt tctagcgcat tcatagcaca atcataacct ttatttccag cttttgtacc
     1861 tgctctttca atagcttgtt caatattttc tgtagttacg attccaaata taacaggtaa
     1921 ttcactgtct aaagatactt tggctatccc tttagagact tcactacata cataatcata
     1981 atgagaagta gcccctctta taacacagcc taaacagatt atagcatcat acttaccact
     2041 tttagccatt ttttgagcaa caagaggaat ctcaaaagct ccaggtaccc aaactatttc
     2101 tatattttct ggagaaacgt catgtcttaa aagacagtct tcagctcctg ataaaagttt
     2161 tgatgttata aattcattaa aacgagaatt tattatacct atttttaaat cttttccaat
     2221 aagttttcct tcatatatca tttttacttc ctccaatttt
//
LOCUS       DAMJZE010000298         2230 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMJZE010000298 DAMJZE010000000
VERSION     DAMJZE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.305"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(134..1426)
                     /gene="brnQ"
                     /locus_tag="PC541_003757"
     CDS             complement(134..1426)
                     /gene="brnQ"
                     /locus_tag="PC541_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2841172.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          745 a          373 c          246 g          866 t
ORIGIN      
        1 aaatttttaa atttatcaaa caggattaat aatgttcttt tatctacaat aatttaataa
       61 ttcttttaaa tataaaaagg aattgcctat ataatttaaa ggcaattcca taattatcaa
      121 ttagttttta ttattatgca gttttactgc ttttagatgg aataaagttt cctattattc
      181 ctgctattac aactggaact atccagttaa atcctaatga agcaaatggt aagctattaa
      241 ctagaggaat ttttacacca aaacttgttg caactgttaa tacacttact aataatgcca
      301 tgtatgcagc acatttgaac acgttgtcat ttttaatttt attactaaat aatgctagta
      361 taactagagt tatagttgct ggatatacca tacttagtat aggagaagag aattgtatta
      421 ttgtacttac tccaaagttt gatatgattg cactaaatac acaaactact ataactatat
      481 gctcatactt taattttcca tctgtaagtg ttgagaaata ttgaccagct gctgaagaaa
      541 gacctatagc tgttgttaaa catgctaatg caactataat agccaataaa attttacctg
      601 tagttccaag taaagctgct gttattgcaa ctattaaagc tgtttgttga acatcttgtc
      661 catattgttg agacacagtt gctccaagat aacataatcc accataaact aatgccattc
      721 ctattactgc aattacacct gcacttattg tggctttcat cttttctttc tcacctgtat
      781 aacctttagc tattactgac atgattataa ttgatgcaaa tacactagct gccaatgcat
      841 ccattgtttg ataaccttga gttatacctt ctgcaaatac attttgtatt aaagaactgt
      901 ctcttatttc tcctaatgga gatacgatac ctttaactat taatactgct aaagctataa
      961 gtaaagctgg agttaatatt gacccaacta tatctacaac ttttgatggt cttattgtta
     1021 aaaccaatac tagagcaaag aatactatag agaatattac tgggcttatt ccagaaccta
     1081 ttgtaggtaa tatacccatt tcataagttg ttgctgctgt tctaggtatt gctaaaagtg
     1141 gccctataca gattaccatt gcacatccta atgcaatact taatcctttg cctgcccttg
     1201 agaacatctt cgatgtgtct ccattgaact tagttgctgc tataactgct aataatgcca
     1261 aaccaccatc tgcaaaaagg aatcctatga aagctactag ccaactactt ccagataaaa
     1321 ctcctagata tggagggaag attaagttcc ctgcaccaaa aaacattgca aatagtgcaa
     1381 atccaattac aattacatct ttaaaattga tttgtttctc acccatttaa agcaccccca
     1441 tttgaatttt actacctttt actaaaaaat aagtccaatc tcttaattaa atgaaaacct
     1501 tttgacgaga ttttatttaa ttatacattt ttgacttaaa tttgtcaatt atgtttcata
     1561 atttataaaa tcttcacaat atttaataca actttcttaa ttaaacaaaa tcaacctgta
     1621 tactttaaac atagttggtt ttttgttttt tctctaattt ttcttgataa aattaactat
     1681 tccgaataat taaaatatta agacttttta gattattcga tttattatta agttgtattg
     1741 ctttatatac tgtatatatc actctccttt tttacattca tataatcttt ttaattcaat
     1801 ttaaatttta aatattttgt gtttattata taatctgaaa aaaaagcagt caatagcatt
     1861 tttaattttt ttaattttca aaattatgat tcttaaaaaa tcgttttcct tttatcataa
     1921 cacctattta cgttaattct tgtagtattt tgtatttatt ttgctaaatt gacagttaac
     1981 tttattcaat ttttattcta cttgcgtaga tttgcctctt gttttttact cttaaaacca
     2041 ttttttctga aaattcctta ttatgattta tcaacttatt tttcttcata acaattactc
     2101 tctaacttcc aacttactta aactttgcag ttgctagtat tcacgtttac atacttatat
     2161 gtcataatta actaaaaatt cttaatatat ctctcaatga aaacaattta ctcatttatt
     2221 taatacattt
//
LOCUS       DAMJZE010000299         2139 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMJZE010000299 DAMJZE010000000
VERSION     DAMJZE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.306"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            95..1807
                     /gene="ptsP"
                     /locus_tag="PC541_003758"
     CDS             95..1807
                     /gene="ptsP"
                     /locus_tag="PC541_003758"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2841173.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT          841 a          217 c          411 g          670 t
ORIGIN      
        1 aaaattataa gtaaataaaa ctataaaaat gtaaattatt tatataaatt tataaagttt
       61 atgtgctaga tgatattttt taggaggtag ggttatggct tacaaaggaa taggtgcttc
      121 tcctggtgtt gctttaggga aagcgttagt agttgaacat agtgaattag taattgaaaa
      181 aaaatctata gataatgttg aagctgaaat tgctaaatta gaagatgcag ttgcagtttc
      241 taaagaagaa ttagtaaaag ttaaggaaaa ggcttctgaa gaattaggag cagaggaagc
      301 agaaatattt gaagcacatt tattagtttt agaagaccca gagttaattg gttctgcaat
      361 agataaaatt aaaactgaaa gtgtaaatgc agagtatgct ttaaatgaaa taaaagagat
      421 gtttgttagc atgtttgagt ctatggacaa tgagtacatg aaagaaagag cggcagatat
      481 aaaagatgtt acaaatagaa tacttagaca tatattagga attaaagttg ttgatttatc
      541 tgctttaagc gaagaagttg tattaatagc acatgattta actccatcag acacagcaac
      601 tatgaataaa aaaatggttt taggattttt aactgatata ggtggaagaa cttctcatac
      661 tgctatcatg tctagaactt tagaaatagc tgcaatcgtt ggattaaatg atataacttc
      721 aaaagttaag gatggagatt ttgtggtttt caatggagac acaggtgaag taatcgtgaa
      781 tcctgatgaa gagactataa ataaatatac tgaattaaaa gctaagtatg aagatgagag
      841 aaaagcatta cagctattaa aaggaaaacc atctgtaact ttagacggaa aacatgtgga
      901 attagctggt aacataggaa cacctaacga tatagaaggt ctaataaaga atgatgctga
      961 aggtgttggt ctttatagaa cagaattttt atacatggat agagattctt tcccaacaga
     1021 agaaatacaa tatgaagcat ataaagctgt attagaagga atggatggaa agcctatagt
     1081 tataagaaca ttggatatag gtggagataa agaattaagt tatttaagta tggaacctga
     1141 aatgaatcca ttcttaggtt acagagcaat aagactttgc ctagatagaa aagatatatt
     1201 taagactcaa ttgagagctt tatatagagc tagtgttcat ggaagattaa gaatcatgtt
     1261 cccaatgatt tcatctttag aagagttgtt acaagctaaa gaagttgtaa aagaagtttt
     1321 agcagaactt gattctgaag gtgtggctta tgctaaagat gtagaaattg gtatgatgat
     1381 agaagttcca tctgctgctg tgatatctga tgtattggct aaacacgtag atttcttctc
     1441 tataggaact aatgatttaa ttcaatacac ttgtgcagtt gatagaatga atcaaaagat
     1501 aagctatctt tacaatcaat ttaatccagc tgttttaaga cttataaaga ctgttataga
     1561 caatgcacat aaagaaggca aatgggcagg tatgtgtgga gaatctgctg gagaccaaaa
     1621 aatgatacca atacttttag gtatgggatt agacgagttc tctatgtctc caatatcaat
     1681 acttcctgct agaaagttaa taacttcagt taaagagtca gatatgaaaa aattagctga
     1741 tgatgtactt aatatgggta ctgctgaaga aataaaaagc tatatagaaa agacttttaa
     1801 tatataatta atgttatata gttactaaaa ttcatttttc taaataacaa aattatattt
     1861 tacgaattaa aaaggagatg tttcaaaata aaaatttgaa gcatctcttt tttataaaag
     1921 attactaaga attacttaat ctttgtaata gtcattttgt gaaaaagcaa aactgcattg
     1981 tggctatagt atatatggat gttattatca ataaaagtac tattaaaatg aagattcatt
     2041 ctcaaatgat atcaaaagta gttgtgatgg agtaagataa tattttgtga tttaatagta
     2101 aaaattaaaa aaatatggta ttgaaaaata attaaaagt
//
LOCUS       DAMJZE010000300         2120 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMJZE010000300 DAMJZE010000000
VERSION     DAMJZE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.307"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            219..1916
                     /gene="ade"
                     /locus_tag="PC541_003759"
     CDS             219..1916
                     /gene="ade"
                     /locus_tag="PC541_003759"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2841174.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT          830 a          221 c          388 g          681 t
ORIGIN      
        1 aaaattcaat atgaattaaa ttattgctta aatattaatg agaacatggg agataatatt
       61 attcctcatg ttctctctat ttaattaaag gaattatgga acaaatttca agtgatactt
      121 tctatcaaaa ctcatatata atatatatta ataaattaaa ctataatacg taatacagtc
      181 agtatattga tataaaaaac ttaggaggag ttgtaaaaat gggtaattta aaaagacagt
      241 gtgatgtttc aagtggaaga gaaaaagctg acattgtact aaaaaatggg acaatcataa
      301 atgtatttac agaagagtta ataacaggag atgtagcaat agtaggagac actattgttg
      361 gaattggcga ttacaagggc aatgtagaaa ttgattgtag taacaaatat atatcacctg
      421 gctttattga tgcacatatg catattgaat ctacaatggt aatgccaatt gaattatcga
      481 aaaaattatt aaaatcaggt acgacaacta tcatagccga cccacatgag ttggtcaatg
      541 taaaaggagt tagtgcaata gattttttat tagaatcgac aaaagatata ccacttaatg
      601 tatatattat ggtgccttca tccgtacctg caacaagttt tgaaactaat ggagttggta
      661 aatttagtgc aaaagacatg gagagttatg taaataatcc tagaatttta ggtttaggag
      721 aggtaatgtg ttttaatgat gttataaatt ctgaaaatga aatcttggat aaactagagc
      781 tttttaaaaa taaagttgta gatggacatg cacctaatat aaatggtaaa tcacttcaga
      841 catatgtatg tgcaggaata gaaaatgacc atgaatgtat aacttttgat gaagtatatg
      901 aaaaattgag agcaggatta aaaattttaa taagagaagg aagcgcagca aaaaatttaa
      961 agtctatagt aagtggtatg ctaaagcata acttgccaat tgaagaattt atgttttgca
     1021 cagatgataa gcacttagat gatatagaaa aacaaggtca cataagatgg aatataaaat
     1081 gtgcgattga tttaggtatg gaacctgtta gggctataaa agttgcaact tataattctg
     1141 caaaagcata tgggttaagg aaaattggag caataggtgc gggttataag gcagatattg
     1201 ttgtattgaa tgatttagac aaaatggaag tggatagtgt atataaagat ggtaatttgg
     1261 taaacgaaga gatgttttct aattacaatt atgagattaa agataaagaa ttgcttaata
     1321 cagtcaagtt taaatatatt aataaagaaa aaatacagtt aaaagtaagt gaaaaaaatt
     1381 atgtaatgga gattgtacct tatcaaattt tgacaaataa ggtatatgaa tctttaccat
     1441 gtgcagatgg atattttgtt ccaaataaag agtattcaaa actttgtgtt gttgagagac
     1501 atagaatgac tggaaatgta gcaattgctc cactaaaagg atttggcata aaaaatggag
     1561 caattgctac tactgtggcc catgattctc ataatattat agttgcaggg gataatgatg
     1621 atgatattct agtagctata aattacttaa aggaaataca aggtggttat gtaattgtat
     1681 caaatggaaa agttcttgca cacctaagtc ttcaagtagc tggattaatt agtactttta
     1741 cagctgaaga agtacaagaa attactgata atatgttgga gatagcaaga aaaatggaag
     1801 ttccagagta tgtagaccct tttattacac tttcttttat ggcacttcca gttgttccac
     1861 aaataagact tacagattta ggtctatttg atgttgagga atttaaattt atatagatag
     1921 tataaaagta ataataaatg aggctttctc aaaatagaga taagtctctt tttattgttg
     1981 aaatgatgaa aatatatttg atttcataca aaaaagagca tctcatgaac taaaaatttt
     2041 atttttagat atccttattt taattaatct atcatttaac attgttttaa gcatagatat
     2101 cttaataaaa ctattttcag
//
LOCUS       DAMJZE010000301         2096 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMJZE010000301 DAMJZE010000000
VERSION     DAMJZE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.308"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            335..1195
                     /locus_tag="PC541_003760"
     CDS             335..1195
                     /locus_tag="PC541_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2841175.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(1434..1991)
                     /locus_tag="PC541_003761"
     CDS             complement(1434..1991)
                     /locus_tag="PC541_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2841176.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
BASE COUNT          765 a          272 c          272 g          787 t
ORIGIN      
        1 attttgataa catataaaag taagataaat ttttccatta aataccttat aattatacac
       61 aaaatctcta tatacgttaa acaatataga gaaaattttc atagaaccat ctttataggg
      121 gatactttat aaaagaataa aaaaattata atatataaaa aacttcatag gagtgtaaca
      181 acatcagtaa agatatgaaa acactatata aaaattcaaa aatattaaga ataatgaaat
      241 acttgaaatt gcttatatca agaaatttta tataatataa actggtcaaa tatgttataa
      301 tattaaattg tgacaagttc atggaggtgt ttatatgaaa aaacaaaagt cgccaatttt
      361 acttgaaatg cttggactat tttttggttg tatatcaatg agtattggta taaatatgtt
      421 tttaaaacca catacaattg ctcctggagg acttagtggt ctatcattag tactaaacaa
      481 ggttacagga cttccagtat cagctatcat gttaattata ggtgtcccat tagtaatact
      541 tgcttttaga attatgggta caaaaaattc attaaaaact ttatttggaa cagttgtatt
      601 ttcagctata gttcaattaa cagacccatt atcaaaacta agatttacta atgacttatt
      661 attgtcttct attgcaggag gaatcctagt tggtataggt ttaggaataa tgtttaaatc
      721 agatgcttct actggaggaa cagacttaat tgcactcata cttagtaaga aattcccagg
      781 aataaaagct acaaaattca tgtcatgcct agatggaatg gttgttatat cttctggtat
      841 agtaaatcgt agtttagaaa ctgctcttta ctctggtata gcactttgtg tattaataaa
      901 aatagcagat atgataatgg aaggatttga ccattcaaaa gcatttttta ttatctctga
      961 tgagcctgaa agtttaagac aagctataac tgaagaactt gatagaggtc ttactattct
     1021 tgatggtaaa ggtggataca ctagagaaaa taaagaagtt ctattagtcg tagtttctaa
     1081 aaaacaagaa ctttatctaa agagattagt taaaagaact gaccctactg catttgttat
     1141 tgttacagat gtacatgagg ttcttggaga aggatttaaa aatttagaga actaataaaa
     1201 attaatattt taagtatata tactatttta attaaaatac gatttattca aaaagagtgt
     1261 ctcataaact ttttagttca tgagacactc ttttttgtat aaaatcaaat gcattttcag
     1321 tattttaaca ataaaaacag gattatctat attttgaggc aaccacttta ttgaaatttt
     1381 atttagaaaa caatcattac taaataacca gcatagatat taaatgatta tatctattgt
     1441 tttttcaatt catattgata acaaggaaca ttttttacag tatttccagt atgaattttt
     1501 ttgatatact ttcctgtaca tttaaatcct aataaatcta atgattttgt agaattttgg
     1561 ttttcttcat aggttattgc aattatttta tttaagttta gttccctaaa tccatactgt
     1621 attactcttt ttgttatctc agtagcaaaa tcatgtcccc aatagcttgg atttatacat
     1681 atacctattt ctgcttctaa tgctttagga tttatattat taaaccccat attcccaata
     1741 actttattgt ctcttttata tactatagca aagtggccaa ttttcccttt tttatatgga
     1801 tttagaaaaa atgattttat aaactttctt gtatcttcta aagatttatg ctcactaaat
     1861 ggtaaatgtt ttactactat tttattttta taacagtcaa aaaaatcttc aacatcatct
     1921 aatgatgcag gtcttagtat acaacttcta gtttctaagg ttttcatttg aggatattca
     1981 gaatcagaca tttctaatcc ccctttatac attaatctaa atatactgtt catatttaaa
     2041 atacgatatt aattatataa atgttctcaa attaacctta tttttatttt taataa
//
LOCUS       DAMJZE010000302         2028 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMJZE010000302 DAMJZE010000000
VERSION     DAMJZE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.309"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..159)
                     /locus_tag="PC541_003762"
     CDS             complement(<1..159)
                     /locus_tag="PC541_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDF2841177.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITKEDYTNL
                     ETQLNTANE"
     gene            complement(204..434)
                     /locus_tag="PC541_003763"
     CDS             complement(204..434)
                     /locus_tag="PC541_003763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841178.1"
                     /translation="MKEYMIWFKSGNSISGIVDEDVADKLMQDFMEADSDCKDLKVYL
                     DEDGTTVIDLSQIEAISINNCNENNNIGFSKS"
     gene            complement(477..674)
                     /locus_tag="PC541_003764"
     CDS             complement(477..674)
                     /locus_tag="PC541_003764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841179.1"
                     /translation="MFYLLLQVISICLSITVFMYVTGNIVFKIYLKSLRKEYESNIHE
                     VSDEILKRIRKELNKSLDESL"
     gene            complement(675..1067)
                     /locus_tag="PC541_003765"
     CDS             complement(675..1067)
                     /locus_tag="PC541_003765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841180.1"
                     /translation="MAKFKKKSEEVEAFKWILGSPNTPIWFYQAFEKGTIWLDESLNS
                     VNRKGEVKKTICIKYKNGVIRATNGDWIIKDSEGKIYSCTCDEFEKYYKDLDNETKIS
                     NGGLECLREIALKDVNFKFELTGIKEEK"
     gene            complement(1399..1560)
                     /locus_tag="PC541_003766"
     CDS             complement(1399..1560)
                     /locus_tag="PC541_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841181.1"
                     /translation="MNCPVCGKDVDVFDICDNCNWQNSGPKETNYKGPNKMSLKEAKE
                     AYEKGKKVI"
     gene            complement(1563..>2028)
                     /locus_tag="PC541_003767"
     CDS             complement(1563..>2028)
                     /locus_tag="PC541_003767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDF2841182.1"
                     /translation="CRTTTAPWFEDEEGYRAARDENGKTYYVPSGMKYKEWYEKTFIT
                     DKKDDILSNKSWLKADFPTEKKFKKHVEKHLNEYGNITAEEYLSTARNLLSEQLSNDV
                     EGFLSKDNFLFKYRKSTNDFAVGRADGKISTLFKPKEGYNYWLEQIKEYKEV"
BASE COUNT          639 a          382 c          166 g          841 t
ORIGIN      
        1 ttcattagct gtattaagtt gtgtttcaag atttgtataa tcttctttag taattgtagt
       61 ctcttttatt ttcttttcta tagatttttg aagagaagct acatcaattt tgttatcctc
      121 tacttttatt ccttctagca attcttttaa ccaatccatt ttaaatatct cctttcattt
      181 tttacaaaat aaaaaagccc tatctaggac ttactaaaac caatattatt attctcatta
      241 caattattta ttgatatagc ttctatttgt gataaatcta ttactgttgt tccatcttca
      301 tctaaatata ctttcaagtc cttacaatct gagtcagctt ccataaaatc ttgcattaac
      361 ttatcagcaa catcttcgtc tactattcca gatatgctat tgccgctttt aaaccaaatc
      421 atatattctt tcaataacag aaacctcctt ttgttttcta cctaataaaa aacttcttaa
      481 agactttcat ctaatgattt atttaattct tttcttattc tctttaaaat ttcatcagaa
      541 acttcgtgta tattagactc atattctttt cttaaacttt taagatagat tttaaagact
      601 atattacctg taacatacat aaatactgta atacttaaac atatacttat tacttgtaac
      661 aacagataaa acatttattt ttcctcctta attcctgtga gttcaaattt aaaattcaca
      721 tcttttaatg ctatctctct taaacattcc aatcctccat tagaaatctt tgtttcatta
      781 tccaaatcct tatagtactt ttcaaattca tcacatgtac aagaataaat tttaccttca
      841 ctatctttta taatccaatc tccattagtt gccctaataa ctccattttt atattttata
      901 cagatagttt tctttacttc acctttacga ttcacagagt ttaaagattc atcaagccat
      961 atagttccct tctcaaaagc ttgataaaac catataggag tattaggact tcctaatatc
     1021 catttaaaag cttctacttc ctctgatttc tttttaaatt tagccatatt atttatcccc
     1081 ctttaaattt ttaatcatat cttcattgct aactagtaaa aaaaatagta tcagtatcac
     1141 acctaaaata aaattaagca gtggaaataa cgctgggaaa ataaaattat atttttttct
     1201 tcttctgata ttcttacttt taagtaattc aattaattcc tcattactat caaccttcat
     1261 gttgaataaa taaagccctg tacaaaacac aattattgat aaaataagta tagatataat
     1321 ttaagcattt caaatcaccc tcgcaataaa tttttacata ataaaagcac ctactaattt
     1381 ataacttagc aagtgctttt atattacttt tttacctttt tcataagcct ctttagcttc
     1441 ttttaatgac attttatttg gtcctttgta attagtttct tttggtccac tgttttgcca
     1501 attacaatta tcacaaatat caaatacatc tacatcttta ccacatactg gacaattcat
     1561 gtctaaacct ccttatattc ttttatttgt tctagccagt aattataacc ttcttttggc
     1621 ttaaataagg ttgatatttt accatcagct cttccaacag caaaatcatt agtactcttt
     1681 ctatatttaa ataaaaagtt atctttactt aaaaatcctt ctacatcatt acttaattgc
     1741 tctgataaca aatttcttgc agtagataaa tattcttcag cagttatatt tccatattca
     1801 tttaagtgtt tttctacatg ctttttaaat ttcttttcag ttgggaaatc tgcttttaac
     1861 caactcttat tacttagtat atcatctttt ttatcagtta taaatgtctt ttcataccat
     1921 tctttatatt tcataccaga tggcacataa tatgttttcc cattttcatc tcttgctgct
     1981 ctataacctt cttcatcctc gaaccaagga gctgttgttg tcctgcaa
//
LOCUS       DAMJZE010000303         2017 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMJZE010000303 DAMJZE010000000
VERSION     DAMJZE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.310"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            86..262
                     /locus_tag="PC541_003768"
     CDS             86..262
                     /locus_tag="PC541_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841183.1"
                     /translation="MKTLMFILVIAYYITIVIKSITTIVKSITRLLDSISRLKESLHK
                     LRNRPDGNQDDFNE"
     gene            complement(481..657)
                     /locus_tag="PC541_003769"
     CDS             complement(481..657)
                     /locus_tag="PC541_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841184.1"
                     /translation="MFKNKMDKCTHMLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(712..809)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1125..1406
                     /locus_tag="PC541_003770"
     CDS             1125..1406
                     /locus_tag="PC541_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2841185.1"
                     /translation="MKEKYKWRKSTTEILLGRLVKMRILKKKRVGFQLNYEVLVTKKE
                     YLDVIKEEKDVSKYDNFFTQVFTTIHKKEEKTEKQIKVFIESMQKLGKK"
     gene            1446..1832
                     /locus_tag="PC541_003771"
     CDS             1446..1832
                     /locus_tag="PC541_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2841186.1"
                     /translation="MLNKKLPDSEFKIMKYIWNTGYKTVISKDVADEMEKIYKWKQTT
                     TLTLLLRLTKRGFLASQKIGKHTHYTILIKEKEYLKSETKKLFGGLHNNPLSQLISKL
                     HDKEEVSIDKIDALEEWLRSLQEDEE"
BASE COUNT          864 a          223 c          291 g          639 t
ORIGIN      
        1 aaatgcaaga atatatcttg caagaggcta caatctaagg gcgaaagttt tcggaaatca
       61 ccttcgaacg aaagggggtg agtatatgaa aacacttatg tttatacttg ttatagcgta
      121 ttacattact attgtaataa aatctattac aacaatagta aagtctataa caagattatt
      181 agattctata agtagattaa aagaatcttt acataaacta agaaatcgtc ctgacggcaa
      241 tcaagacgat ttcaatgaat aaatcattac ttaaattatt ttgaatttga aacttcgctc
      301 taggctaata gattgtagtt tctttttttg tttttgatac gaatttacat ctttattata
      361 tcgcatttta agaaaaaata aaactatgaa tattataaat ctattttaat taatttttta
      421 tcatatcatt atttaaaagt agataaaact aaaaagggtt ctctagaaat caatgtctat
      481 ctaattatac ttacttacca ataccatcac ttggtgtaaa caagactcta ctacattttc
      541 tccgtactct aatataaaat catctaactg tgtatctaaa aaattacaat aatcatacga
      601 actgctaata taagcagtca acatgtgtgt acatttatcc attttatttt taaacatata
      661 aattattatc cccctaaaaa actttcataa aaacaaaaag aacactatct ttttggcaac
      721 tggctgacat aactcataag atatttatat aagtcttagt ccctatagct ttgcgtcact
      781 aaatttctct agttttgccg atttagtttt attctacaac taaaataata caatagaatt
      841 agttattgtt caacaagatt gtttgaaaat taaataaatt tgtagatgtg aataatattt
      901 tttatatata aaaacaaact aaaacagtag aatatatggt aaaaattacc tatagttttt
      961 gttctaaaaa gctcctatta ttaatttaag ttagacgaat taaggaagtg acataaatgg
     1021 agcgacaaga ggtagaaagt aaattacgca ggggcgaatt aatgttgatg gagtacatgt
     1081 gaagggaaaa gtctactcta tctaaaaaag aaatagaagt agcaatgaaa gaaaagtata
     1141 agtggagaaa aagcactaca gagattttac ttggaagatt agtaaaaatg agaatattaa
     1201 aaaagaagag agttggtttt caattaaact atgaagtatt agtaactaaa aaagaatatt
     1261 tagatgtaat aaaagaagaa aaagatgtaa gtaaatatga taattttttt acacaagtat
     1321 ttacaactat acataaaaaa gaagaaaaga cagagaaaca aataaaagta tttatagaaa
     1381 gtatgcagaa actaggaaaa aaataatttt atatttttat atgttaatag aaagaagggg
     1441 atattatgct aaataaaaaa ttaccagatt cagagttcaa gattatgaaa tacatatgga
     1501 atactggtta taaaactgta atatcaaaag atgtggcaga tgaaatggaa aaaatatata
     1561 aatggaaaca aactacaaca ctaacacttt tattaaggtt aactaaaaga ggttttttag
     1621 cttctcaaaa aataggtaaa cacacacatt atacaatatt aataaaagaa aaagaatact
     1681 taaaatcaga aactaaaaaa ctatttggag gtttacataa taatccttta tcacagttaa
     1741 tatcaaaatt acatgataaa gaagaggtaa gtattgataa aatagatgcg cttgaagaat
     1801 ggttaagaag tttgcaagaa gatgaagaat aagaatatgc caaaaggtag cactcaatag
     1861 tagctacctt tttgtggata aagctgggga taaactgtgg aaaagttata taaaaattgt
     1921 ggataaaatg ttttgctgac atttaactga cataaatatt aaaacttgat tttaaagtat
     1981 tgaaatttca attattaatc atataaaaat agaaaag
//
LOCUS       DAMJZE010000304         2005 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMJZE010000304 DAMJZE010000000
VERSION     DAMJZE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.311"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            93..>2005
                     /locus_tag="PC541_003772"
     rRNA            93..>2005
                     /locus_tag="PC541_003772"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          589 a          387 c          577 g          452 t
ORIGIN      
        1 taatcgctag tgttcgaaag aacactcaca agattaataa ctagttttag ctatttattt
       61 tgaataacaa ttcaaaatat ggtggaaaca tagattaagt tattaagggc gcacggtgga
      121 tgccttggca ctagaagccg atgaaggacg ttactaacga cgatatgctt tgggtagctg
      181 taagtaagcg ttgatccaga gatttccgaa tgggggaacc cagcatgagt tatgtcatgt
      241 tatcgacatg tgaatttata gcatgtcaga aggcagaccc ggagaactga aacatcttag
      301 tacccggagg aagagaaaga aaaatcgatt ccctgagtag cggcgagcga aacgggaaga
      361 gcccaaacca acaagcttgc ttgttggggt tgtaggacac tctatacgga gttacaaaag
      421 aacatgttag acgaatcatc tggaaagatg aatcaaagaa ggtaataatc ctgtagtcga
      481 aaacatattc tctcttgagt ggatcctgag tacgacggag cacgtgaaat tccgtcggaa
      541 tctgggagga ccatctccta aggctaaata ctctctagtg accgatagtg aaccagtacc
      601 gtgagggaaa ggtgaaaagt accccggaag gggagtgaaa gagaacttga aaccgtgtgc
      661 ttacaagtag tcagagcccg ttaatgggtg atggcgtgcc ttttgtagaa tgaaccggcg
      721 agttacgatc tgatgcaagg ttaagcagca aatgtggagc cgcagcgaaa gcgagtctga
      781 atagggcgtt gagtatttgg tcgtagaccc gaaaccaggt gatctaccct tggtcaggtt
      841 gaagttcagg taacactgaa tggaggaccg aaccgactta cgttgaaaag tgagcggatg
      901 aactgagggt agcggagaaa ttccaatcga acttggagat agctggttct ctccgaaata
      961 gctttagggc tagcctcaag tgatgattat tggaggtaga gcactgtttg gacgaggggc
     1021 ccctctcggg ttaccgaatt cagacaaact ccgaatgcca attaatttaa cttgggagtc
     1081 agaacatggg tgataaggtc cgtgttcgaa agggaaacag cccagaccac cagctaaggt
     1141 cccaaaatat atgttaagtg gaaaaggatg tggcgttgcc cagacaacta ggatgttggc
     1201 ttagaagcag ccatcattta aagagtgcgt aatagctcac tagtcgagtg acactgcgcc
     1261 gaaaatgtac cggggctaaa catattaccg aagctgtgga ttgtcctttg gacaatggta
     1321 ggagagcgtt ctaagggcgt cgaagcatga tcgcaaggac atgtggagcg cttagaagtg
     1381 agaatgccgg tgtgagtagc gaaagacggg tgagaatccc gtccaccgat tgactaaggt
     1441 ttccagagga aggctcgtcc gctctgggtt agtcgggtcc taagctgagg ccgacaggcg
     1501 taggcgatgg ataacaggtt gatattcctg taccacctag tatcgtttta atcgatgggg
     1561 ggacgcagta ggataggcga agcgtgctgt tggagtgcac gtccaagcag taaggctgag
     1621 tgttaggcaa atccggcact cataaggctg agctgtgatg gggagaggaa attgtttcct
     1681 cgagtcgttg atttcacact gccgagaaaa gcctctagat agataacagg tgcccgtacc
     1741 gcaaaccgac acaggtagtc aagatgagaa ttctaaggtg agcgagcgaa ctctcgttaa
     1801 ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctctttag ggttcacgcc
     1861 cagaagagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcta
     1921 aaccgtaagg tgatgtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
     1981 gttagcttct gcgaagctac gaatc
//
LOCUS       DAMJZE010000305         1977 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMJZE010000305 DAMJZE010000000
VERSION     DAMJZE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.312"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            75..473
                     /locus_tag="PC541_003773"
     CDS             75..473
                     /locus_tag="PC541_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841187.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    909..1172
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1323..1874
                     /locus_tag="PC541_003774"
     CDS             1323..1874
                     /locus_tag="PC541_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2841188.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          831 a          186 c          298 g          662 t
ORIGIN      
        1 ttgaaaaaat ttgtaatttt tagttatata tatattataa tgaactagtt aaaaattttt
       61 atgaggggga aaatatgaat aagaagaaaa aattgattgg gatagctata tcttttctac
      121 ttataatcag cattataaat ccattacaag tagaagcaga agctattatt gagtctagtg
      181 tatacatttc ttcaataaaa gagaattata catatgataa tactaaattt aaaactatta
      241 aatctaaaag tagacataaa agtaggaaat ataataaaag taaagtaaag ttgaaaaaac
      301 gaagtataag aactaaaaag tatggatatc aaactataaa taaatataaa agacaaaacg
      361 caagaagaca taatatccta acaaaattaa tattgtccat aatagtcata tttttggtta
      421 tagttttagt agtgctaata tttattaaaa gaaagaagaa aaaattttac taaaaataat
      481 taaaagaata tcattttttt aatcattggt atctatttaa taatatgatg taattaaaat
      541 ggagtaatat tctgtttcaa aggaggcata taaatacagg aatgagtatt aaatgatggt
      601 taattaagta aatttattga taaatgtact taattaactt tgaagaattt aattcctcat
      661 gaatcaaaat ataaaattac atcaacagtt attcacaaaa ttaatataag aagaattaga
      721 gccaaagagc agactattag accattaaag taattattca tagaattttg tatatgaaaa
      781 gaaatgtata gtataattta ctttttttat aaaaaataga ttacaaaata tattttttat
      841 aaaaaggtat tgacattttt tagaaaaaag aatatcatta ttttaacaac aaaattcaat
      901 acaataaatt aaatcctatg acaaggaaga gtagttaaat ttaggttcag tatagagagc
      961 tgaggatggt ggaatcttag caagaaaaaa tttgatgaat ggcccttaga ggaataaatt
     1021 gaaatcatag ttttgagagt agactttatc gtcagtcgca cgttatagcg aataaggtat
     1081 gtaagtacct gataatgaga tgtatgaaaa ttgaatttta tattaatttt tatataattt
     1141 aggtggcaac gcggataatc tccgtcctat tagtttagga cggagttttt tattttactg
     1201 aaaatttaaa agatagaagg tggtgagaaa gatgttatga atttcaaaag ctaaagatga
     1261 tgatatagta ggttggcaaa aaaataaatt gaaaattaaa aaaataggag gaagtacaaa
     1321 aaatgaaaac aaatacaaaa aaattaactt taaatgcaat acttttagcg atgggattat
     1381 taatacatca aatcacacca gctataggat taccaatgaa gccagatgta ccactagcaa
     1441 tgttatttgt aatattggtt ttaaatagag atgattataa aacttgtttg atagcgggta
     1501 tagtaacagg aatatttaca gcacttacat caagttttcc gggaggtcaa attcctaatg
     1561 taatagataa aactcttaca gcaaatatag tatttttatt aatgaatatt agttataata
     1621 tgccttttat aaaaaaatta gctaagaaaa cacaagatac tgtagtagtt gcaataataa
     1681 tgccaatagg aactcttgta agtggaacta tctttttatt agcagctcaa gctatagttg
     1741 gtcttccagg ggcttcattt acagctttat tcttagcagt agtattacca gctgttttaa
     1801 taaatctagt tgcaggaata tttttattca agattgttag tctatcaata agaagagtta
     1861 gttatcaagc ttaaacacga aataatattt tactttataa agcattataa taactaaata
     1921 acataagaat aaagtagatt ataactattt tattcttatg ttgtttaaag atttggt
//
LOCUS       DAMJZE010000306         1964 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMJZE010000306 DAMJZE010000000
VERSION     DAMJZE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.313"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            84..458
                     /locus_tag="PC541_003775"
     CDS             84..458
                     /locus_tag="PC541_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2841189.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            455..1318
                     /locus_tag="PC541_003776"
     CDS             455..1318
                     /locus_tag="PC541_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2841190.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1318..1956
                     /locus_tag="PC541_003777"
     CDS             1318..1956
                     /locus_tag="PC541_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2841191.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          750 a          206 c          301 g          707 t
ORIGIN      
        1 ttttttctaa aatcagttga catattatat atatatttat ataatgtgta tatactatat
       61 atacaattta aggaggtgtc ttattgaata tagttattag taattcgagt gggaagccaa
      121 tatatgaaca gattactaca caaattaaaa gtatgattat aagtggagag ttaccagaag
      181 gctcagctct tccaagtatg agactattgg caaaggaact tagaataagt gtaattacta
      241 caaaacgagc atatagcgat ttagaacgag acggatttat tgagacagtt actggtaaag
      301 gcagttttgt atctagtaaa aacatggatt ttgtcaaaga agaacaattt cgtttaattg
      361 aagaatacct acaaaaagca gttgatacag caaaaagtag tgatattaca tatgacgaat
      421 taaaagatat tttattttta ttatataaag gagaatgata agtatggatg caataactat
      481 aaaaaattta aataaaactt ataaagattt tagcctacaa gatatatctt tttcagtacc
      541 aaaagggagt gtaatgggat ttgtaggtga aaatggtgca ggaaaaacaa ctacattaaa
      601 agcaatttta aatttaatat cttatgatag tggaaatata gaaatctttg gtcttgatag
      661 taaaaaaaat gaaaaagaaa ttaaagagca aattggggtt gtatttgagg gaagcaactt
      721 tcatgaaaat ttaaacactg accatgtttc aaaaatcatg agtaaaatct ataaaaattg
      781 gaatgacaca ttatttaaag actacttaaa aaaattgaga gtaccagaca ataaattaat
      841 taaggagttt tcaaagggga ataagatgaa acttagtatt gctgtagcct tatctcataa
      901 accaaaatta ttaatactag atgaggcaac tagttcactt gaccctatcg ttcgtgaaga
      961 aatactagat atatttttag attttataca agatgaagaa cactctatta tcttatcttc
     1021 acatattaca agtgatttag ataagatagc agactatatt acatttatac ataaaggaaa
     1081 aattgtattt agtgaaaata aggatgaatt aattgatact atgggagttt taaaatgtaa
     1141 acctagtgat tttgataacc tttcaagaga ggattattca tattatcgta aaagccaatt
     1201 tggatatgaa gtacttctta aagataagca tagatttatc tcaagacatc caaactgtat
     1261 tgttgataat acgtctatag aagaaattat gttattttat gtgagaggtg ataaataatg
     1321 aaaggtctaa ttttaaaaga tttacttaat ttgaaaggaa atataaagtt tatactttta
     1381 ttcatcatta tgtttggctt tatgtcttct ttaggagatg gaaatgttaa taactttata
     1441 ggtgttatta tagttttatg tactacaatg attgtttcaa cattttcata tgatgatttg
     1501 aataaatggg atagttatgt acttacaatg cctattaatc gcaatgatat tgtattaagt
     1561 aaatatttaa ctatgttaat atttagcttt ataggtgttc ttgtttcact tattgttagt
     1621 gtaactattg gttattttaa aaatacactt atattaaatg aaacactact tattaatgca
     1681 ctaatcttat caatttcagt atgttttggt agccttattc taccacttat ttataaattt
     1741 ggtacagaaa gagctcgttt attaatgatt ttatgctttt tagttccaac actagcttta
     1801 ttagttttta aaagtatttt agaaaatatt agttcaccta tttctataga aattatactt
     1861 aatacactag tatattctct tccatttgtt gctattttac tatttgtgat ttcatatttt
     1921 atatcatcaa aaatctatag taaaaaagaa gtataaaata tact
//
LOCUS       DAMJZE010000307         1936 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMJZE010000307 DAMJZE010000000
VERSION     DAMJZE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.314"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            125..1336
                     /locus_tag="PC541_003778"
     CDS             125..1336
                     /locus_tag="PC541_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2841192.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1529..1789
                     /locus_tag="PC541_003779"
     CDS             1529..1789
                     /locus_tag="PC541_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2841193.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT          814 a          173 c          344 g          605 t
ORIGIN      
        1 ttttgataaa ataagaatga atttgtgcaa ttttattttt ctccaataac taaaaatagt
       61 tttttgaatt aatatttgct taagaataaa aatcagaaac gtttttgata tagtaggtga
      121 taatatgaaa ggattagttt tagaaggtgg aggaacaaaa ggagcctacc aaataggtgc
      181 atataaagct ttaagagagc ttggtataga gtttcaagga gtaactggca catcaattgg
      241 tgctttaaat ggagcatata tagttcaaaa tgatattgaa gtaatggaag atatttggtt
      301 aaattatgac tatactcatt ttatgaatat tgatagagaa acctatgaga ggtttaaaaa
      361 tgtagatttc actactaaaa atctcaatgc agtgtttgag cttataaata aagctagaaa
      421 aaatgaaggt atagatataa gcccattaag acaactaatg gagacaacat taaatgaaga
      481 cataataagg aaatcaaaaa aagactttgg acttgttacg gtctggtggg ataaaaaaat
      541 taatccatat ccattgtata tcgaagatat acctaaagga agattgattg attatttaat
      601 tgctagttcc agtttgccta tttttcaaac gaacatgatg gatgataagt tgtatcttga
      661 tggaatgttt tgtgataaca tgccaatagg actcttggaa gacaaaggtt atgaagatgt
      721 tgttgtaatt aggttgattg atgattttat tgggaaaatg aatctaaatc gacatcaaaa
      781 tataaatata aaaactataa taccatcaga atatctagga ggttctctta ataaagatag
      841 agataatgta gaaaaaaaca tccgactagg ttacttagat actatgaaag catatgaaag
      901 atgcactgga attaagtatt attttgatgt agagtataaa tataatgaag aatattgttt
      961 tgataaattt agaagtatga gcaatgaaac aatacaatat atctgtaatt tattgaatat
     1021 aaaaagagat gcttcactta gaaccgttat ggaaagtgta ataccaaagc ttggtgaggt
     1081 gcttggattg ccaaaggatt tttcatataa agatttgttt tactgtatat atgaaaagaa
     1141 acttgaagaa aataatataa acaggataaa attatatgat tttaacaagg ttatagatgt
     1201 tgtaaataca aatattgatt ctaatagaaa tttagcttct aattttgaag ttagacctaa
     1261 aattagtatt aaaaagaaca aaaacacaaa attattgaca aattgtatta taaaagacat
     1321 aaaaaatcaa agttaaataa aaaacaaaaa aaaatttgta attatactcg aaaaaagtgt
     1381 atattagtag tataatattt ttgtagcact agtgggaaca aaaaatgacc ataggacaaa
     1441 aaagtaccac ttagtaccat taaagtaaaa aacaggaaaa caattttaaa aaatataaaa
     1501 tgtaaagttt ataggaggat ttaaaaaaat ggaaaaagta gttagtatca aaaatgcaag
     1561 tggattacat gctagaccag caggaatgtt tgttaagaag gcagctgaat ttaaatcaac
     1621 agtagaggtt gtagcaaaag acaaaactgt aaatgcaaaa tcaataatgg gaataatgag
     1681 tttaggttta gctcaaggag aagaattaaa gttagttgta aatggagaag atgaagaagt
     1741 agctttagct gcaatggttg aattagttga aagtggattt ggagaataga tataataatt
     1801 ttttaaaaat ttgatattta gaatctaata ttacttaaag taaagtaaca gagaagtatt
     1861 aaacatatac agaccaacat ttgtagatat ttaatttctt tgttactttc atgtttattt
     1921 aatcagaaga ttaaga
//
LOCUS       DAMJZE010000308         1934 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMJZE010000308 DAMJZE010000000
VERSION     DAMJZE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.315"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            153..347
                     /locus_tag="PC541_003780"
     CDS             153..347
                     /locus_tag="PC541_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2841194.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(449..958)
                     /gene="vanZ1"
                     /locus_tag="PC541_003781"
     CDS             complement(449..958)
                     /gene="vanZ1"
                     /locus_tag="PC541_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2841195.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1278..1667)
                     /locus_tag="PC541_003782"
     CDS             complement(1278..1667)
                     /locus_tag="PC541_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2841196.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
BASE COUNT          713 a          263 c          195 g          763 t
ORIGIN      
        1 ataaatattt tacccataac ctttattttt ttattaaaat gcattataat aaaaagtaaa
       61 tctaaatttg atacagtttt aacaatcatt attttcagaa aagtgatact attgaatata
      121 ttaagaataa ttagagatgg aggaactaag atatgagcgt aattgtaatg tgtagtcgtt
      181 gtggaaaaga aatagataaa tatagaaata aatatataac ttattatgag aaaaatacca
      241 atgcagaaat taaagtttgt ataaattgtg cgttagattt attagaaaaa acaagagaaa
      301 atcaagaatt gaacatacaa gagtttcaag atacaataat tctttaataa tttaggaaag
      361 cacctgtata ggtgcttttt atgtgtaaat aattttatat gtaactgtat cttacgttct
      421 attttaagct aaacatatat acttatttct ataagtttgc aaaaataact aatcctaaaa
      481 ataatacaac aataattgtc acaatcaatg ccaatacatt gataattttt atggtattat
      541 tcttaaatat tttcgataat agcataaaaa cagctattcc tatgattcct cctagtgtgt
      601 ttcctatcaa atccgttata tcacttgcac ctagtgcaaa tatatattga agtgtttcaa
      661 ataccaaact agttataaaa ataggtataa ctttttttat aaagctccat tcttctttta
      721 gcatacacac atatatccca aatggtacaa atatagccac atttaatata atctctgata
      781 tatctactct attattaatt ataagtgacc cagcaaaagg tattaagttt atgcttctaa
      841 gattcatggt ttcaagagat gagatatcaa attgcaactt aaatagtatt atccatgtta
      901 aaataattat gtaaactata aataatcctt ttgtaatatt atgttttcta gatttcattt
      961 attacctcct tactatatta ttgtattatt ttattaatac aataatatag taaggtaatt
     1021 tatctgtcaa ctatttcttc taaaattata ttccttttat aatttcaata tctttatcat
     1081 atttacatga tttaagctat tgtagtattt catatatctt taaattttaa ttggtattat
     1141 tctctatatt ttttactatc aaacactaag gaaataatca cttttcttta tataatgttc
     1201 taccatcaca taaaactaac attaaatcca aacgctactc tcaattgtct caaactaaaa
     1261 agatactgat agctttatta gtcatcaata tcttcttctt tcaaattttt aaagtattct
     1321 tctaatttgt ccaagctctt actatctaaa acttcatcat catgtagtgc tgaaataaaa
     1381 ctcttaaatg aattgttatg cataaaacta aaaaaactct ttgtctcaac ttttaagtaa
     1441 tctttctctt ttatagctat agtataatgc gtacatctat ctaccttatc agtagttaaa
     1501 aagcccttat ctactaaatt ttttaaaaat gtcaatgttg tgctcttttt ccaatcgtat
     1561 ttcaacttca tgtcttctat gatatctctt gaggctaata catcatctac tccccaaata
     1621 tacttcatta gttttagttc tgcatgtggt aatttattaa tcttcattag tttacacctc
     1681 ttaccaattt atgtataact aaactttata acaataagtg tatgtaatca attagattgg
     1741 aataaatata aattccaatt tattctatat aaaaataatt aataaaaaag aagtactcta
     1801 ctttagagag tacttttgga ttatatattc aaaaatttat ctaacatgat tagagtatgt
     1861 tttatgcttt agtgtgataa ttttagttcg ttttattatt gcaacatgta ttatatttca
     1921 ttataatcta taag
//
LOCUS       DAMJZE010000309         1925 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMJZE010000309 DAMJZE010000000
VERSION     DAMJZE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.316"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="PC541_003783"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="PC541_003783"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="PC541_003784"
     tRNA            242..316
                     /locus_tag="PC541_003784"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="PC541_003785"
     tRNA            321..395
                     /locus_tag="PC541_003785"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="PC541_003786"
     tRNA            401..476
                     /locus_tag="PC541_003786"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="PC541_003787"
     tRNA            482..558
                     /locus_tag="PC541_003787"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="PC541_003788"
     tRNA            569..643
                     /locus_tag="PC541_003788"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="PC541_003789"
     tRNA            658..742
                     /locus_tag="PC541_003789"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="PC541_003790"
     tRNA            752..825
                     /locus_tag="PC541_003790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="PC541_003791"
     tRNA            836..912
                     /locus_tag="PC541_003791"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="PC541_003792"
     tRNA            922..997
                     /locus_tag="PC541_003792"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="PC541_003793"
     tRNA            1009..1084
                     /locus_tag="PC541_003793"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="PC541_003794"
     tRNA            1087..1175
                     /locus_tag="PC541_003794"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="PC541_003795"
     tRNA            1193..1283
                     /locus_tag="PC541_003795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="PC541_003796"
     tRNA            1292..1368
                     /locus_tag="PC541_003796"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="PC541_003797"
     tRNA            1454..1529
                     /locus_tag="PC541_003797"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="PC541_003798"
     tRNA            1590..1666
                     /locus_tag="PC541_003798"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="PC541_003799"
     tRNA            1673..1749
                     /locus_tag="PC541_003799"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="PC541_003800"
     tRNA            1753..1828
                     /locus_tag="PC541_003800"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="PC541_003801"
     tRNA            1836..1912
                     /locus_tag="PC541_003801"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          484 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatca
//
LOCUS       DAMJZE010000310         1892 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMJZE010000310 DAMJZE010000000
VERSION     DAMJZE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.317"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            132..416
                     /locus_tag="PC541_003802"
     CDS             132..416
                     /locus_tag="PC541_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841197.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(573..746)
                     /locus_tag="PC541_003803"
     CDS             complement(573..746)
                     /locus_tag="PC541_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HDF2841198.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1187..1402)
                     /locus_tag="PC541_003804"
     CDS             complement(1187..1402)
                     /locus_tag="PC541_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841199.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1413..1880)
                     /locus_tag="PC541_003805"
     CDS             complement(1413..1880)
                     /locus_tag="PC541_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2841200.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          769 a          201 c          236 g          686 t
ORIGIN      
        1 tttaaagcat taaaaaataa atttgtattt tgttttatat aaagtaattt gaagaatatg
       61 gaaaaacttg attagtacat tattaattag ggtataaaat ggtgtaaata gaatgataag
      121 gggtggatga aatgcttgta tataataaaa gtttttatcc taatgacata tttccaagat
      181 tagatttttc aaaaataaaa aaacagttaa aattgataga taatgacctg tcagattttg
      241 gaagcatatg tataatagaa aaagaacatt atacaataag tgtaaacagt ataggtgaaa
      301 taaatgtgta ttatgattta gagtacgaaa ataaggtgta tagaatagtt tatgagattg
      361 aaaagttatt taaatctcaa gttggaaggt ttagcatatc tacatacaga aattgataat
      421 taaaaaagta gaaattaaaa aacttaatac taaagatata gataaatata tgaggtaaca
      481 gctagagaga gttgttaccc tttttaaata ctaagtatat attattctta ttaatttttt
      541 gtaaattcac taaaagacct ttttatagaa tattattttc tttttttatt aaataggtaa
      601 atcagtaaaa atgcaattcc agttccaatt attattccaa tgatattttc tataattaat
      661 ttttttaata taaaagtaaa taataaaaat gctatacata aacctaaaat atatccttta
      721 aaataggttg aaagttttct tttcatataa tcaccaactt gtatcagatt tagtttactt
      781 aattagagta taacagtgtt gtaacttcaa agagtatcta aaagattaca aatgttagat
      841 aaaatcatca ataatgtaat ttataacacg tagctataaa ctatgcaata atataaatgg
      901 aggtataagt aaatgaaaaa gttccatgtt ttgctaacag tttaaataaa tattaggtag
      961 aaaagaatgt atataggaat ctgtggtaaa attatcaatt taaaagtatt atttagtgtg
     1021 atacagttct tataggtaat ggataacaaa tagataaagg aacagtttct ttcttagaac
     1081 atattttgtt atgactttaa acaaagttaa tattaatgtc aataatatag taaaagaata
     1141 aaaatcatat agtataataa aaaagagtgt ttctaaacac tcttttttaa tcattttcat
     1201 cttctacata ttcaagtata tcacctggct gacagtctaa agctttgcaa atttcgttta
     1261 gagtggtaaa tctaatagct tttgcttttc cagtttttaa aattgataaa tttgcatttg
     1321 taatatctat tttttctgcc aaatctttaa gtgatatttt cctttttgcc atcattacat
     1381 ctaggttaac tactattgcc ataacatcac ctctatatcg ttaaatcatt atcttctttt
     1441 atttttatag ctttatcaaa tgtttctgca attacaaagc acattagtgc aaatataaaa
     1501 gagataatca taagtggtga gataccgcca ctatcagttc caaaaattct tatattatta
     1561 gtatttaaat tataaccatt gatacattca attatagtgt ttatcaataa acaaaatccc
     1621 attaccttaa acctttttac attttcaagt acgaatgggg aatacttagt actattaatt
     1681 ataaatatca agaataagat gactataaag tagtcagaaa aatatagtat tcttgcaatt
     1741 aaagcgatta ctttgtcaga actaaaatca tataaaaaca taggtatcat acataagaat
     1801 attataccag caactcctaa aagtgataat agaaaatata gaaatactga taatatatta
     1861 ctaaaaaatt ttgtaatcat aaaatatcat ct
//
LOCUS       DAMJZE010000311         1830 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMJZE010000311 DAMJZE010000000
VERSION     DAMJZE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.320"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            94..663
                     /gene="mocA"
                     /locus_tag="PC541_003806"
     CDS             94..663
                     /gene="mocA"
                     /locus_tag="PC541_003806"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2841201.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            660..1679
                     /locus_tag="PC541_003807"
     CDS             660..1679
                     /locus_tag="PC541_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2841202.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          733 a          165 c          348 g          584 t
ORIGIN      
        1 tttatatgat attatatata tagataatat caatagagat attataaaaa tcatagaact
       61 atcaatatat tataaatacc aaggatgata tatatgataa atgcagtgat aatggcatca
      121 ggctttgcca gaagaatggg agcaaataaa cttttattaa attataaagg ctgttctata
      181 atagaacatg tatttagaga agttagcaag attgattttc atcaagttgt agtagtaagt
      241 caatatgaag aggttttgtt attgtgtaat aaatatggtt ttgaatatat agataataaa
      301 aatgcaaata taggtcaaag tgaatctata aaattaggag ttttaaatag ctcaaaatgt
      361 gatggttata tgttctttgt aggagaccag ccatttattg ataacttata tatagataaa
      421 attataaata cttttaaatt agatagagat tttatagtaa ttccaagata caaagatatg
      481 tgtggaaatc cagtcatatt cccatatagc aagaaagaag aattgttggc acttaaagaa
      541 gatgaaaaag gtaaaaccat tttaaaaaat tcatcaaaaa taaagtatgt agaagttcct
      601 aaagaaatgt tatttgatat cgatacaaaa atggactatg agagaattgg gggtaaatta
      661 tgaagctaat aaaaactata gatgctgttg gacaagtttt atgtcacgac ataactcaaa
      721 taatacctgg agaatttaaa ggaagaaaat ttaaaaaggg tcacatagta aaagaagaag
      781 atattccagt gttattatct cttggtaaag ataatttgta tgtatgggaa aagtcagaag
      841 gaatggttca tgaaaatgaa ggcgcattgt ttttaaaaga actaactgct ggagaaaatt
      901 tagattttag tgaaataaaa gaagggaaaa tagattttat agcagcatgt gatggtctat
      961 taaagataga tgttgatgca ctttttgatt tgaattgtgt tggagaaata atgatggcta
     1021 ctcttcataa taattttata gtaaataaag gtttaaaagt agctggaaca agagtgatac
     1081 ctttagttat agatgagaaa aaattagaag atgcaaaaaa agtagttggt aatagaaaaa
     1141 tagtaaatgt agtacctttt aaacctaaga aagtaggaat tgttactaca ggaaatgaag
     1201 tgttttatag cagaattgtt gataaatttg gaccagtaat agaagaaaaa gtaaaaggct
     1261 ttggttgtga agttatagga cagactatat gcccagatga taaagaaatt ataaagagtg
     1321 caataaaaga atttataagt caaggagcag aacttatctg ttgtactggt gggatgagtg
     1381 tagacccaga tgatgttacg ccaacagcta taaaagaaac tggagctgat ttagtgacat
     1441 atggttcacc aatattacca ggagctatgt ttttacttgc ttattacgga gaagttccaa
     1501 ttatgggtat accaggatgc gcaatgtatc acaaaactac agtatttgat atagttttat
     1561 ctagggtttt aattgatgaa aagcttgata agtatgatat agctagatat ggtcatggtg
     1621 gcctttgtat gaactgtgat gtatgtactt atcctgcatg taattttgct aaaatataaa
     1681 gttattaaaa aagagtattt acaaattaag tactcttttt aaatatgaaa caaaacagct
     1741 agaatgatgt tttaaaaaaa gcatatttat ttagaaatta taataactta ttttcaatgt
     1801 gtaaaagaat gaataaagtt gcaatttgat
//
LOCUS       DAMJZE010000312         1807 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMJZE010000312 DAMJZE010000000
VERSION     DAMJZE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.321"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(297..1625)
                     /locus_tag="PC541_003808"
     CDS             complement(297..1625)
                     /locus_tag="PC541_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2841203.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          658 a          328 c          207 g          614 t
ORIGIN      
        1 agatttaatt ttcataaaag ctatgtataa tatttgttgt acagatacca cttagtttga
       61 taaaaaacac tttaaaataa agtattttaa aataaataat tttttagatg tttaatttta
      121 taatcaaaaa actaaaaata aaatgaatac cttaaaataa actttttagt tcatggaacg
      181 ctcttttttt gtaaaattaa acatatcttt accattttaa caataaaaaa gagacttatc
      241 tctatttgag acagtctctt ttattttaat tatgaagcat ttgtaaacta cttggtttat
      301 ctttttactc ctctagtcat ataagcatac actggtaagc ctatagctgt tattacaaga
      361 cctcctatag atatcatagt agttttcata cctgcaaaac acaattgatt aactacaaca
      421 aatataccac ttgctatagc tattatagga acaataggat ataatggaac tttatactct
      481 cttggtatat ttggatgagt ctttctaagt ttcataaccc ctataaaagt taatacatag
      541 aatatccaag tagcaaatac agctaagtca gttaataaat taaattgacc tgacaaagca
      601 tatatagatg caataacaga tactaatgct attgcatttg ctggaacttg agcagaattt
      661 aatttagaaa atataccata ctttggtaaa gtcttatctg tagctaaagt atatgccacc
      721 cttgaaccag ttagtaaaaa tccatttaat gctccaaaca ctgaaattaa tatacctaca
      781 gatattattt ttccacccat tgaaccaaat ataacttcag caactgctga tgcaggagaa
      841 gcataatttg ctaattcatt agctggtaat acccaaagat acgctaaatt tattataaaa
      901 taaacagcca taacaactga caatccacct attatagctt ttggaaggtc ttttccagga
      961 ttcttcattt ctccagctaa agtaccaaca ttcatccatc catcaaaagc aaataatata
     1021 gcaattaaaa cttgacctag tacactaccc aggcttaatc cttcaccaac cataggtgta
     1081 agtattggat tatttccacc acctttaata aatccaacaa ccattattaa aataagtggt
     1141 attaacttac aaattgtaga aacagtttgt atgactcctc cagtttttga acctaacata
     1201 ttaagccctg caactattat tataattcca attgctattg gaagtaaaag tgatggacta
     1261 ccaattagtg ccgatgcttg ttctccaaat ataacaccta atgctgccat catccctgga
     1321 taaaataata caatttgcat ccatccagtt aaaaaaccta atttttctcc ataaatttct
     1381 tttatatata ccatcattcc accagttttt ggtatcgcaa ctgatacctc tgcagctgta
     1441 agaccagctg ttatagttat gattccagct attaaccatg ccaatatacc aagacctgga
     1501 gctcctccag ttaatgtata gactgcttgt ggtttaaaaa atacgcccgc tcctataacg
     1561 gaaccaacaa cagttgataa tgctgctgat acgccaagag tctttttcag ttcattactt
     1621 cccatttttt tcctcctcat taatcctatt taattttaat ttcatagtta aataagcata
     1681 tattatgcca atatatagaa aaatagcttt attatttatt aaaataaaat aaactctact
     1741 tagctttagt aatcttttat ttttagttat gtttaaatat aaaaaaatta taaaaaccaa
     1801 aaatcca
//
LOCUS       DAMJZE010000313         1763 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMJZE010000313 DAMJZE010000000
VERSION     DAMJZE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.322"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(762..1079)
                     /locus_tag="PC541_003809"
     CDS             complement(762..1079)
                     /locus_tag="PC541_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841204.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(1389..1757)
                     /locus_tag="PC541_003810"
                     /pseudo
     CDS             complement(1389..1757)
                     /locus_tag="PC541_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          630 a          225 c          178 g          730 t
ORIGIN      
        1 atagcaaatt tttagggtat aatttttaaa attatattct actatcaaaa gatatgaata
       61 aatgattgta tatattatat caatttttat atttcatagt ccattttttc atcacatttt
      121 cgcttgtcaa gaataatatc tttaagagtg aagcgtttga tgtaaatctg gactttgttt
      181 ttgagacatt tttgaaatat ttattaaatc tttcgattat acttgtaata tatattatag
      241 atattactta ttttagtaaa actaaaggat gatgctacaa cattccagtt atttcccaaa
      301 attttaattt ctgaagtata ctgtttacat tatttttaat ctattttatc taaagtcgat
      361 aaggcttctt attcattaac tgacctatat acagatttaa aattacttat taattctttt
      421 gagtccttgt atgaaacata tttaaagtat ttcttatcta atgtattaaa catttttgta
      481 ttacagaatg aggataactt gtagctattg tctctttgaa ataagtcatc tgtgtttact
      541 cctaaaacta cttatatctg tagattttat aagttctcca tcttatataa tttctttgat
      601 taattgtttt cttaaattat tcataaaaaa atctccgtta caatatttga ttatatttaa
      661 atcattgcta taaagaagac ttttattaac tttgaaaaac aaattatttt aattgctggt
      721 gattagttgc taccttcttt tgatattaat tctattagat tttatatagt tatatatctc
      781 tttctaacta agtaatataa tattactgtt atagtagcta ctaatattat tgctattatt
      841 gtaatatcta atctttttgt aaataatcct attgctatat attcaaaacc tgttacatat
      901 cgataaagta cttctagttt aatagttctt tttctatcta tatttccata ttttgattct
      961 aatttattaa aatatttttc attacaaaaa ccaattttaa tttcaattaa agatattatt
     1021 gtagttaata ttataattcc caatcccata agtataagat aaatgttcct aaaattcata
     1081 gaagatacct cctttataat ataatcatca actcatgttg tattaatttt cctaatcaaa
     1141 gtataacagt aacacaagta taaagagtat ctaaaatatt acaaatatta aatgagaaaa
     1201 agatggtaag ttaaccccac catcttattt tttatctact aatgaatgat gctacaaaag
     1261 ttagtataaa aaatgaaaca aacaatataa tgaacttgtg taatttactt agttggaatg
     1321 ctttattaca aaataataaa attaatgctg aaacaattgc aaaaagtaga tattttataa
     1381 tcattttatc acctattgat ttctacagat tgtaagtagt atttggttac acaagcacct
     1441 cctttattat aacgtatttt ttaaatttaa agatttttcc agatgttata tttgactcaa
     1501 ggtacattgt aattaatcta actataagtg gcaagtactg tcctatacca ggaatagcac
     1561 cagccaaaag tcctgaaatc cctataccag catcagttaa agttaatgca actcctttag
     1621 aaagatataa attaaaacct attctagccc aaactatttt atttatttca gcttttctat
     1681 aatataattc aagtagtgtc aatatcgttc ttcttaaggt ttttaattag cttatatatt
     1741 gttattgtat aatataatta tta
//
LOCUS       DAMJZE010000314         1736 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMJZE010000314 DAMJZE010000000
VERSION     DAMJZE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.323"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            123..1613
                     /locus_tag="PC541_003811"
     CDS             123..1613
                     /locus_tag="PC541_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2841205.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT          669 a          184 c          351 g          532 t
ORIGIN      
        1 aaacactata tatattcgat aaaataaaat ttaaacatat aatattttat gtatatatta
       61 ataaaaggac atattgtgtt ataatggtat cgtataaaat catttacggg ggtggtatta
      121 aaatgtttca atttgaaaca caaatacaca aattaagaca tgatgtttta actgcaatag
      181 ctagacttgg aaaagaagat aaccttacca gagaagcaat ggataatatt cagtatgaaa
      241 taataaaagg tgagaaacct acatacagat gttgtgttta taaagaaaga gctataatat
      301 ctgagagagt aaatgtaagt atggggttac aaccaggtca agataagata gatttagata
      361 caatggatat agaagaagat gaacaaatta tatatatttt ggaagctgca tgtgacagtt
      421 gtccaataaa tgaatttaca gtaacagata tttgtagagg atgtctagca catagatgta
      481 aagaagcttg taaatttgga gctataacac atgtcggagg aatggcctat ataaatcatg
      541 aattatgtaa ggcttgtgga atgtgtaaaa aagcttgtca atatgatgcc atctcagagg
      601 ttgttagacc atgtaagagt gtttgtccaa ctaatgcctt aggatttgat agagaaaata
      661 tgaaagctat gatacatgag gataaatgtt taaactgtgg agcatgtatg tcagcatgtc
      721 catttggagc aatatctgat aagagtttga tagctcctgt agctagaaag ttagttcaaa
      781 aagagaaaat gtatgcggtg gtagctccag ctataacagg tcaagttgaa gcaaatgtta
      841 catatggtca aataaaaaat gctataaaat cactaggatt tgttgatgta ttagaagcag
      901 cttgtggagc agatgcagtt acagttcatg aaagcttaga atttgctgaa agaatggaaa
      961 agggagaagc ttttatgaca aattcttgtt gtccaggatt tgttggttat atagaaaaaa
     1021 catacccaaa tcatgtaggg aaaatatctt ctacagtatc tcctatgata gctagtggta
     1081 gatttataaa aagtcatgaa ccagatgcta aagttgtatt cataggacca tgtacagcca
     1141 aaaagagtga ggcagctaag aaagaattaa aaggagctgt tgattatgta cttacttttg
     1201 aagaaataat ggcattattt gaggcatttg atatagactt ggctaagtgt tcaaatgaag
     1261 atatagatga tgcatcaatt tttggaagag gttttggagc tgctggtggt cttacaaaag
     1321 ctatagaaaa ttatcttgct gaaaaaggta ttggtgttaa ttttgaacct gtaaagattt
     1381 ctggttcaag agaaatcaag aaaactatga ctatggctaa tttaggaaag ctagaaggta
     1441 attttataga aggaatgatg tgtgaaggtg gttgtataaa tggttctggt aaattcttat
     1501 caggtgcaaa ggttaaagcc acttttgata gaaaaaattc tcaatcaact aagaaaagtg
     1561 tcttagcaaa tacaaagata aaagatttta gtaatataaa tttagaaaaa taaagataat
     1621 acaaagcaac aaaaatacat attgttaata gagttgttct aagttgaggt ttaacttttg
     1681 gacaactcta tttttattta tatatttaat tattgaacaa ctaaaataaa aattat
//
LOCUS       DAMJZE010000315         1712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMJZE010000315 DAMJZE010000000
VERSION     DAMJZE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.324"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            120..1358
                     /locus_tag="PC541_003812"
     CDS             120..1358
                     /locus_tag="PC541_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2841206.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          730 a          147 c          284 g          551 t
ORIGIN      
        1 agttgttaaa ttaggaaata ttaaaaaaaa tatatattta tatttaaact attttgttat
       61 ttatataaaa atacttatct agttactagc aattattagt agaaatgggg gaaatagaga
      121 tgaataaggg attttacaaa ttaaattcag tggtattgaa ctttgatatt aagaatgatg
      181 ttaacaagat aagatttaat cattatataa tgagaaaaga aaacatacaa agacttcaag
      241 gaactctgcc aaacggacaa ttttatatga cagtgagaag agctgctaca gatttacaac
      301 tttctatttc tactataagt aggcttgtaa aagagttttt ggatttaggg ataataagat
      361 tagtgtctaa gggtgtaaaa ggtaatcgtt ctgtatatag ttacgtaagt atagagttta
      421 atggaagttt atttgatgat agtaaatata tggaacttga ctgtgtatgt aatgatgaat
      481 ttattgataa tagtggcata gagtttgatt gtgatagcaa agataattct tttaatggag
      541 atataaatgt agaaactgtg cattgcatta atgagtattt ctctgatgat gatgtagagc
      601 ataattataa caatttccat aacaaagata ctataaagga taaagccatt tctaatatta
      661 gcactatcaa aaaatatatt gatgaatctt ttgatacaaa taaatcagaa aattctaact
      721 tacaacagga gtcaataaat ttcaacttta atgatacatg caatgaaata gataaaagaa
      781 cgaataatag tatagaaaat aatcaaaata aaaggcctat gagattgagt gacatcagtc
      841 ttttaagtga taaaggtatg acaaataaaa agaattactt taatcataat catggtatac
      901 aaaaagggac taaatctgta acaaagaaaa aagaatcctt aaataaattt aataaaaaaa
      961 atatacagca aagcattata aatatgctaa ataaaaaatc aggtaagaat tttaaaatcg
     1021 attctccaat aagcataaaa cttatcaatg aaaggctaaa ggaaggctat gtcttagaag
     1081 atttctacaa agttattgag gtaaaagtgg ctaagtggaa aaataccata atggaaatgt
     1141 atataagacc agaaactttg tttagctata agtttgaatc ctatgtaaat gaaaacatat
     1201 ctatgaaaag tcaaagtaat gttcacccat gtaaagaagg caatagtaat gatagcagta
     1261 attatataca ttctgattat ataacatatg atggcagtgt gcgtgagaga aaaaaatatg
     1321 agaatgtaag atactggaat cctatatgtg aaatttaatt agtttaaaaa tgtatttatg
     1381 ataaataaga tattaataga gaatagtgag ctatagcaaa tgatatatca atagttggta
     1441 gtaagttata ttaagtaaga tattactatc aggtagtgaa ttatattgaa cgatatatta
     1501 ataattggca gtaagttata tcaaataaga tattactata gagtaatgaa ttatattgaa
     1561 cgatatatta ataattggca gtaaattata ttaaataaga tattactata gagtaatgaa
     1621 ttatattgga cgatatatca atagttggta gtaagttata ttaagcaaga tattactatc
     1681 aggtagtgaa ttatattgaa cgatatatca at
//
LOCUS       DAMJZE010000316         1682 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMJZE010000316 DAMJZE010000000
VERSION     DAMJZE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.325"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(84..1634)
                     /locus_tag="PC541_003813"
     rRNA            complement(84..1634)
                     /locus_tag="PC541_003813"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          388 a          475 c          359 g          460 t
ORIGIN      
        1 aatgttaata aacattcaaa actgaataca atatgtcacg ttatccctca tcttcgtaga
       61 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
      121 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct ccaaatggtt
      181 actccaccgg cttcgggtgt tacaaactct cgtggtgtga cgggcggtgt gtacaagacc
      241 cgggaacgta ttcaccgtag catgctgatc tacgattact agcgattcca gcttcatata
      301 gtcgagttgc agactacaat ccgaactgag aacaacttta tgggatttgc ttgacctcgc
      361 ggtttcgctg ccctttgtat tgtccattgt agcacgtgtg tagcccaaat cataaggggc
      421 atgatgattt gacgtcatcc ccaccttcct ccggtttgtc accggcagtc aacttagagt
      481 gcccaactta atgatggcaa ctaagcttaa gggttgcgct cgttgcggga cttaacccaa
      541 catctcacga cacgagctga cgacaaccat gcaccacctg tcactctgtc ccccgaaggg
      601 gaaaactcta tctctagagg ggtcagagga tgtcaagatt tggtaaggtt cttcgcgttg
      661 cttcgaatta aaccacatgc tccaccgctt gtgcgggtcc ccgtcaattc ctttgagttt
      721 caaccttgcg gtcgtactcc ccaggcggag tgcttaatgc gttagctgca gcactaaggg
      781 gcggaaaccc cctaacactt agcactcatc gtttacggcg tggactacca gggtatctaa
      841 tcctgtttga tccccacgct ttcgcacatc agcgtcagtt acagaccaga aagtcgcctt
      901 cgccactggt gttcctccat atctctgcgc atttcaccgc tacacatgga attccacttt
      961 cctcttctgc actcaagttt tccagtttcc aatgaccctc cacggttgag ccgtgggctt
     1021 tcacatcaga cttaaaaaac cgcctacgcg cgctttacgc ccaataattc cggataacgc
     1081 ttgccaccta cgtattaccg cggctgctgg cacgtagtta gccgtggctt tctgattagg
     1141 taccgtcaag acgtgcatag ttacttacac atttgttctt ccctaataac agagttttac
     1201 gatccgaaga ccttcatcac tcacgcggcg ttgctccgtc aggctttcgc ccattgcgga
     1261 agattcccta ctgctgcctc ccgtaggagt ctggaccgtg tctcagttcc agtgtggccg
     1321 atcaccctct caggtcggct acgcatcgtt gccttggtaa gccgttacct taccaactag
     1381 ctaatgcggc gcggatccat ctataagtga cagcaaaacc gtctttcact attgaaccat
     1441 gcggttcaat atattatccg gtattagctc cggtttcccg aagttatccc agtcttatag
     1501 gtaggttatc cacgtgttac tcacccgtcc gccgctaacg tcagaggagc aagctcctcg
     1561 tctgttcgct cgacttgcat gtattaggca cgccgccagc gttcatcctg agccaggatc
     1621 aaactctcca taaaaattat gatgtttgat tagctcataa atactaaata gtttgtaacg
     1681 tt
//
LOCUS       DAMJZE010000317         1679 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMJZE010000317 DAMJZE010000000
VERSION     DAMJZE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.326"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            109..1545
                     /gene="miaB"
                     /locus_tag="PC541_003814"
     CDS             109..1545
                     /gene="miaB"
                     /locus_tag="PC541_003814"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2841207.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          715 a          171 c          303 g          490 t
ORIGIN      
        1 ttactattaa aacacaacta gaaaaataat aatatcttta aaagattttt cttatttgat
       61 ataattaagg gtatgtgtaa tataagaaat taactaggag gaatagacgt gagcaaaaga
      121 aaacaagtaa caatacctat agagaaaata caagaacaag ataaatatat agaacaaata
      181 cacagacaaa atgaagaata cttcaacaga actggaaaga gaaaactcgt gtttactcaa
      241 acctttgggt gtcaaatgaa tgaacatgat tcagaaaaat tatgttcaat gttagaagag
      301 atgggatacc aaatgtcaat gatggttgaa gaaagtgatt taataatata taacacctgt
      361 gcagttagag aaaatgcaga gctaaaagta tatggtaact taggacaatt aaaacatcta
      421 aaaggaaaaa atccagatat gaaaatagct gtatgtggat gtatgatgca acaaccacat
      481 gttgtagagg aattaagaaa aaaatataaa catgtagacc ttatatttgg aacacataat
      541 ctatataaat ttccgcaatt acttacagaa tcaataaatt ctgacaaaat gttagtggat
      601 gtatgggatg tggatggaga agtaatagaa ggtcttagaa gtaatagaaa atttgaatta
      661 aaagcatttg taaatataat gtatggatgc aataatttct gcacatattg tatagtccca
      721 tacacaagag gaagagaaag aagtagaact cctgaagata taattaacga gataaaagag
      781 cttgttgcaa atggaacgaa agaaatcact ttacttggac aaaatgtaga ttcttatggt
      841 aagacacttg aaaatccagt ttcattctca gaacttttaa gaaaagtgaa tgatatagaa
      901 ggaattgaaa gggttcgatt tatgacatct catccaaaag atatatctga tgaagtaata
      961 tacgcaatta gagattgtga taaggtgtgt gagtttttac atctaccaat acaatgcgga
     1021 agcagtagcc ttttgaaaaa aatgaataga cattatacaa aggaatatta tttagaaata
     1081 atagaaaaag ctaaaaagga agttccaggt attgcttttt ctacagactt aatgattggt
     1141 ttcccaggag aaacagaaga agatttattg gacactttag atgtggttga aaaagttaga
     1201 tatgattcag cttttacatt tatttattct aaaagacagg gaactcctgc tgctaaaatg
     1261 gaaaatcaaa taccagaaga tataaagcat gatagattta atagggtgtt agaagctgta
     1321 aataggattt ctgcagaaat aaatgatgga tataaagata gaatagttga agttctagtt
     1381 gaaggaagaa gtaaaaataa tgaaaataag tttgctggta gaactagaca aaataaactc
     1441 gtaaatttcg aaggtggaaa tgatgattta attggtaaat tagttatggt caaaattact
     1501 gaacctagaa ctttttcatt aaatgggata ttagtaaata attaatattg tacaaaataa
     1561 taaaattatg taaactaatc aaaatagatg tactatagaa caaattatat taaaaatttt
     1621 tattttaagc gaatttgtca tatgttatgc taatgtaagt ttttgctatt aataaaaat
//
LOCUS       DAMJZE010000318         1666 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMJZE010000318 DAMJZE010000000
VERSION     DAMJZE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.327"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            205..1608
                     /locus_tag="PC541_003815"
     CDS             205..1608
                     /locus_tag="PC541_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2841208.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT          701 a          184 c          313 g          468 t
ORIGIN      
        1 ttttgaactt ataacttaat taaataaaat aaattataat gttttgaatt tatagagaat
       61 aaaaacttta ggtatattgt ttgagttgta tggataacta tggtatcata tataaaatat
      121 ggtaacaaaa ttgtaacttt tgattaaaaa taagtatgag tactaataaa aaatatgaaa
      181 agtacattgg gggtagaaaa taatatgaag aatagtaaaa agatattagc tataggactt
      241 acactatttt tagtaatggt aaatactcct atggtaaatg cgttgacatc agttgagcaa
      301 ataaaaggga atgacagata tgagacagca gcaaaaatag cagataagca aaactacaat
      361 acagcaatac taatcaactc agataatagc ttagcagatg gtctaagtgc aagtggttta
      421 gctggagcct taaatgcgcc tattttgatg acaaaacaaa atcagattcc aaacacaact
      481 atggaaagat taaacaaagc aaagactgtg tatataatag gctcagaatc aacaataagt
      541 aaaaatgttg agaatcagtt actatccaaa aagaaggttg tacaaagaat ctttggtgaa
      601 aatagatttg atacaagtat aaaaatagct gaaaaaatca aagaaattaa gccaatagat
      661 aaagtaatta tagccaatgg atttacagga gaagcagatg cgataagtgc atcaccagta
      721 gctgctagag atggagtacc tataatactt acagatggaa atagtgtggg atttgacacg
      781 acaggcttaa agagttatgc gcttggttct agtgaaataa tcagcgacga attagttaag
      841 agcacaaatt ctattagatt gggtggcaca gatagatttg aaactaataa gatagttata
      901 caagagtttt ataaaaatag taaagaattt tatctaagta aaggcttaca attaacagac
      961 gctttggctg cctctacaat agcaaaaaat gctcctgtag ttttagttga aaatggcagt
     1021 aacaagagta ttttaagtgg tgcagacaaa ttgactgtac taggaggcat aaatcagaat
     1081 gttatcaagc aatgtataaa tcaagcttca ccaaatcaac aaggtttata ttacaatcca
     1141 aatgatagag catttaaaga gagaataaag ggtaaagtat atgctcttac aaagcagtat
     1201 agaaaagaga atggagtaag agcgttatct gtagcaagca gattagaggg tttagcaaat
     1261 gattggtcta atttaatggc aaataagaag actttatctc atacgataaa tggaaaaaat
     1321 tcatattcta cttttttgaa atacttagat tggagtgaga ttaaacctgg atatatagca
     1381 gttcaaggtg aaaatataat taaatataaa attccagata aacctgtata tacaaataga
     1441 gatgcagatg atataggaaa ctttattttt aatgagtgga agacaaatcc agaagaagga
     1501 actaacatgt tgcataaagg gtatgaaata atgggatttg gtatagctat aacaggagat
     1561 aagaatctat atgctacaca tgaattttat ggaagatata aagaataaga aaatatacac
     1621 aaaattatgc ttgtaaggaa gctatataaa taattaaatt ataatg
//
LOCUS       DAMJZE010000319         1649 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMJZE010000319 DAMJZE010000000
VERSION     DAMJZE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.328"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            184..1524
                     /locus_tag="PC541_003816"
     CDS             184..1524
                     /locus_tag="PC541_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2841209.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          653 a          123 c          266 g          607 t
ORIGIN      
        1 aaaaagcaat aaaaagttta attcaattta gcaaatttag tgatataatt aaaaaagaaa
       61 agaaatatgg acttatttgg aataattttg acaatctatt tactacatgt gtagagtcgt
      121 atataattaa attatacata aaatataaaa aaataattta gtaatacgtt aggagaaagt
      181 attatgataa agtcaagctt attttggaat attttcattg cgatatcctt tatgatagaa
      241 tggtttcttt gtaagaaaat gctagattat acaagtgaga aaaggatttc taacaaaaaa
      301 ataaactttt gtatggtagg tattattgtt tttgctgaca taatatattt ttttgatgta
      361 cctccaaata taagagtaat tatatctatg ataattacta ttgcatttta taaaatctca
      421 tatgatgtgg ggtttataaa gtctgtatta atatccttaa tttattggat gttactaatc
      481 gccacagatg ctttgagtat atctttaaca gtttggctta attctataaa tgacatgagt
      541 agtatgggga cgggagatat atacagaatt gagtctataa tattgggaaa gatacttttg
      601 attgtgtctt tgcttttata tagagcctta aagataaaac ttaatataga aataaataaa
      661 aggacattat tttatatagt tattcctata gtcgctaata tagcatcttt ttttgtaatt
      721 tttgaataca tatttaatcc agggtctggt agcttaatac ataatttcca attcgttatg
      781 atttctgttg tattgcttat atcaagtatc actttagttt taattgctaa aaaattacag
      841 caatataata ctttagttgt agaaaacaat atgattaaaa atactttgaa gtatgaaaat
      901 gagtattatg aagatataga aaagaaatat ttaaaaacta gattactaag tcatgatatg
      961 aaaaatcaca tattgtgtat aaatgctatg attaaaaaag gtattgatgt tacttcttat
     1021 ttaaataatt tgcaggtaga aattcagagt aatgacttgt tatttaatac tggtaatttt
     1081 atattagatg ctatattaag tgaaaagaaa gaggtttgtg ataagaatga tatagaacta
     1141 aaaataggaa taaattttag ttgttgtgaa gctgttgaat tagtggatat atgtcatata
     1201 ttttcaaata ttttagacaa tgcaattgag gcatgtaata aaattgatag tggaaataga
     1261 gttatttgtg ttaaaggtga tgttatgcaa aatttttatt taataagagt tgaaaatact
     1321 aagataaata agattattac taaaaatgga gatattttga cagacaaaaa aggttctctt
     1381 catggacttg gaataaaaag tgtaaaaaac tctgtaaata agtatggtgg taatgttgta
     1441 attgaatatg atgataaaaa atttgtagtt aaaattgcta ttccacttag aaatgtgaag
     1501 aaagcagtta atcatgtgat ttaaatgtat ataaatttga tgtgtagaaa tttaatatat
     1561 aagaattaat gtataaaagt gatgcataga agtttaatat ataagaatta atgtataaaa
     1621 gtgatgcata gaagtttaat atataagaa
//
LOCUS       DAMJZE010000320         1590 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMJZE010000320 DAMJZE010000000
VERSION     DAMJZE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.329"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            43..1479
                     /locus_tag="PC541_003817"
     CDS             43..1479
                     /locus_tag="PC541_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2841210.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          647 a          162 c          269 g          512 t
ORIGIN      
        1 atttatatat taaaaattta ttatgagggg gacaatttta ttatgaaatt ctataaaaga
       61 atattaacat tgactttaag tatttctttt gtatttgcaa atattcaatt agtagatgca
      121 atatcatctg tagaaaaaat acaaggaaaa aataagtatg aaatcgcagg taaaatagca
      181 gataaaaatg catataaaac tgctatatta attaatacta gtaactctat tgcagatgga
      241 ttaagtgcaa gtggactagc aggagcttta aatgccccta tactattaac tgaaaaaaat
      301 actataccaa ctgagacatc tgcaagatta aaaaatgtat caaaagttta tataattgga
      361 ggaacatatt ctattagtac atctgtcgaa aattcattaa aatctaagaa aatgaaagtt
      421 gtaagaatta agggaaatga cagaataaaa acaagttata atgttgcaaa agaaataaat
      481 tcaataaaaa aagtcaatac tgttatgcta accaatgcat ataaaggaga agcagatgct
      541 ataagtattg catctgtagc agcaagagat aaagcaccaa taattcttac taatggacaa
      601 agtatacctt tttcaactag tggtttaaag tcttatgtaa taggaggaac agcttcaatg
      661 agtaccactt tagtaaacaa tactaaatct actagactag gggggagtac tcgttttgaa
      721 actaataaag caataataaa taagttttat aaggatgcta gagagtttta tatagctgga
      781 gcttatgaat taacaaatgc acttgtagga tcttctttat caaaacatgg tccaatggtt
      841 ttagttaatg atggtagcaa taaaagcatt ttaaaaaatg ctaagaagat aacttctata
      901 ggatatatag attcaaatat agtacaacag tgtttaaata taacaaatgg aattggagat
      961 attaatactg gggttgtaaa aaatgttaaa cctactacaa agactataaa agatggaatg
     1021 tataaggttg gaaaagatat ctcagcaggt gaatacttaa taacttcaaa ctcaggaagt
     1081 tatgcttctt attatgaagt tacaagtgat agtacaggta atgctgatag tatactttca
     1141 aatgatattt ttagtgggac tagatatatc accttaaaga atggtcaata tattaaaatt
     1201 gaggattcaa caatgacatt agcaaagtat gctaaagccc agaaggccaa gaatggtaag
     1261 tttggaaatg gtatgtataa aattggatta gaaattcctg ctggagaata tattataatg
     1321 tcaaattctt ctgatgcata ttatgaagtt agaaatgata gcttaggtaa tgcagaggga
     1381 atagttacta atgatacttt ttcaggaaga agatatataa cggttgagga aggtcaatat
     1441 ttaatattaa atgattgtta tttaattgaa aatgaataga tgtaattgta tttaatagta
     1501 aatcatatat tttatttaag gaaaggtgtt ttaagtttta atttaaaaca tctttttatt
     1561 attataataa ttatatgatt ttatatttta
//
LOCUS       DAMJZE010000321         1573 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMJZE010000321 DAMJZE010000000
VERSION     DAMJZE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.330"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            12..410
                     /gene="tnpA"
                     /locus_tag="PC541_003818"
     CDS             12..410
                     /gene="tnpA"
                     /locus_tag="PC541_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2841211.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            407..1570
                     /locus_tag="PC541_003819"
     CDS             407..1570
                     /locus_tag="PC541_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2841212.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
BASE COUNT          673 a          166 c          279 g          455 t
ORIGIN      
        1 tgttagtttt tatgtataaa tctaacaaaa atatagttta tgattgcaaa tatcatattg
       61 tattttgccc taagtatcgt aaacctgttt tagttggcga agttgaaaag atgttaaaag
      121 aaatactacc atataaagca gatgaacttg gagcagaaat aatagaaatg gaaactgata
      181 aagaacatgt gcacttatta atatcttgtg accctcaata tggaattcat aaagtagtaa
      241 aaggtttaaa aggctttagt tcgagagtat taagagagaa ctttccacat ttaaagtcta
      301 gtatgcctag catgtggact aatagctatt ttgtaggtac tgttggttct gtgagtttag
      361 aagtagttaa acaatatata gaaaatcaac aagttaggac aaagaaatga taaaaactca
      421 tagagtgaaa ttaaacctaa ctagaacaca atttgaatta gtaagggaaa agcaaatgga
      481 aagtgctaac tgttggaact acatagttaa cttatctaaa gaatactatt ttgaacataa
      541 acaatggatt aaaaagaatg atatacaaaa gttaatcaaa ggtaagtaca atcttcatag
      601 tcaaactatt caagctatat cagataaatt tgatgcaaat agaaagacta tatcagaatt
      661 aagaaaaaaa ggtaatacta aagcaaaata tccatacaag actaagaaat tttatataat
      721 accttttaaa gctagtgcta ttaatagaaa tgctaaaggc aacttaaaat tatcaatgtc
      781 aaaaggcagg tatttagaat tagattttaa tgttgagaat ataaaaactg ctgaaatagt
      841 atggcgaaat ggatattatt tatactacac ttttgacaat gaattaaatg gagttgtagc
      901 caaaggcact aataccgtag gtgtagactt aggggaaata cattcgatag ctagtgtaac
      961 aaataaaggt gtagggctta ttctatctaa tagagaaggc agaagtatta aacaatttag
     1021 aaataagatg tatgcttata tatctaaaag attaagtaaa tgtaaaaaag gtagtagaca
     1081 atcaaagaag ttatggagat taaaaaataa gataagaagt aaaacagata atcagttaat
     1141 gaatttatat catcaaacaa ctagaaagtt tatagacttt tgtgtagagc agaaggtatg
     1201 tgaaatagtt cttggggata taaaaggggt tgaaaaagat actaagaaaa agaaaagatt
     1261 aaacagagtt aatagacaga aaatatctca aatggagtat ggtcgaataa aggactatat
     1321 aaaatataaa gcaaaagagc aaggtattga agttaagtta gttaaagaaa actacacttc
     1381 tcaaacttgc ccaaagtgtt ctaaaaaaca caaaccaact ggtagaactt atagttgtac
     1441 ttgtggttat gaaactcata gagatatagt tggtgcttgg aatattttaa ataaaaagca
     1501 taaatatggt ttagtagatt ttagaataaa tcataagcaa ccaataaatt taaaagtatc
     1561 taccgtttga gcc
//
LOCUS       DAMJZE010000322         1526 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMJZE010000322 DAMJZE010000000
VERSION     DAMJZE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.331"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            11..484
                     /locus_tag="PC541_003820"
     CDS             11..484
                     /locus_tag="PC541_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2841213.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(853..1410)
                     /locus_tag="PC541_003821"
     CDS             complement(853..1410)
                     /locus_tag="PC541_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2841214.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          545 a          210 c          167 g          604 t
ORIGIN      
        1 gttgtttata atgttcaaag aatacttaat aagagaatta aaagaagatg atattaaaaa
       61 tgcattagac ttaatctgga acgtatttga agaatttgaa tctccagatt attgtgatga
      121 aggaattacg acatttcatc aatttataga atataactct attattgaaa aaatcaaaga
      181 aaaaactctc ttcttttggg gttgttttga ctctaaaaaa cttattggag tcttagctat
      241 tagaaattta aatcatatat gtatgttatt tgtagataaa aactatcata aacatggtgt
      301 tgcaaaaaaa ttaatgacta caggcataca atactgtatt tgtaataaat ttaatttaac
      361 tgaaataact gtaaattcat caccttatgc tgttgaagtt tatcataaat taggatttaa
      421 agatactgct gttgagcaaa ttgttgacgg tatacgtttt acacctatga aattgttaat
      481 ataataaatt aaataaaaag caaagtctct taaatatttt atagttactt tgcttttaaa
      541 ccaagattta aaataatact atcttttttg ttttttaata atatacttta aaatccttac
      601 taatatgaac tataatttat tttttgatat cctttttgat taacatataa attaattcaa
      661 ttattaaaca taaactatgt tgtttctctt tatatctctt ctttgtaaac aatattgggc
      721 atttgaaaat aaatattctt aaaatatact gttctcctgt tgattttcct ttggtacaaa
      781 tgataaaaat ttaatttaca tgccccataa tgaagtgttc aataaataag ttaaaataat
      841 ttatttacct ccttaatcat attttttatg aatacctaat aacacagtat caatagaatc
      901 ggctaaacct tttatagctt cttctttact caaaagtgat ttcccaagtt gcaaagtcga
      961 ataaccatga agtacactca ttattaagtt taatacttca cctgtattct ttgtatttag
     1021 attacaagaa agaataagtt tttctaataa ggatttatat tcatcaaaga tttctaatgt
     1081 ttcattattt ccatgccaag tagcccactg aattgtttca taaacaccag gattagaaat
     1141 tataaaatta aagtatgcta tacttataca ctttatagct gtatctccaa aatttccaat
     1201 agtcgccttt atcatttgac tattcatctc tctcattcct ttatgagcta cttcaagtaa
     1261 taaatcatct agactttcta tatgattgta caaagatggt gtacgtattc ctagcttttc
     1321 tgcaataact tttaaagaaa ctttgttaag cccatcttta ttaactatat ctgatgtagc
     1381 ttgtataact atttctctac taacacgcat gattttccct ctttcattta aaattaatta
     1441 ctttattagt ttagctaatc taattagttt cgtcaattat tgtaatttat aaaattcttt
     1501 atactattat aaaatcttga cagttt
//
LOCUS       DAMJZE010000323         1526 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMJZE010000323 DAMJZE010000000
VERSION     DAMJZE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.332"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..642)
                     /locus_tag="PC541_003822"
     CDS             complement(<1..642)
                     /locus_tag="PC541_003822"
                     /inference="COORDINATES: protein motif:HMM:NF023069.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2841215.1"
                     /translation="MSEKEIWDKVLEIAQERISNTSYQTFIKDTQLYSLKNDEAIILV
                     SLPFNASWLNQRYSEIMQAIIYDVIGYEVKPHFISEDELASYNNVNTQEVQEPQVQHS
                     SIDDKTWGKEQFNMHNTFDTFVIGPGNRFPHAASLAVAEAPAEAYNPLFIYGGVGLGK
                     THLMHAIGHHVLSNKPNAKVIYTSSEKFTNEFIKSIRDNETEAFREKYRKIDVL"
     gene            1364..1501
                     /gene="rpmH"
                     /locus_tag="PC541_003823"
     CDS             1364..1501
                     /gene="rpmH"
                     /locus_tag="PC541_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2841216.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
BASE COUNT          511 a          229 c          243 g          543 t
ORIGIN      
        1 taaaacatca attttacgat acttttcacg aaatgcttca gtttcattat cacgtattga
       61 tttaataaat tcgtttgtga atttctcact agaagtgtaa atgactttag cattaggttt
      121 gttgctaaga acatgatgcc caattgcatg cattaaatgt gttttaccta gacctacgcc
      181 tccatatata aataatggat tataagcttc tgccggtgct tctgcaacag ctaaacttgc
      241 agcatgtggg aaacggttac caggtccaat gacaaatgta tcgaatgtat tgtgcatatt
      301 aaattgttct tttccccaag tcttatcatc tatagaagaa tgttgtactt ggggttcttg
      361 aacttcttgt gtatttacat tgttgtagga tgcaagttca tcttctgaaa taaaatgtgg
      421 tttcacttca taaccgatga catcataaat aatagcctgc ataatttctg aatatcgctg
      481 attaagccaa ctcgcattga aaggcagact tactaatata atggcttcgt catttttaag
      541 tgagtagagt tgcgtatctt ttatgaacgt ttgataacta gtgtttgaaa ttctttcctg
      601 ggcaatttct aaaactttat cccaaatttc tttctctgac ataaataact ccatctttct
      661 taaaatttta gacttataca cagaaataac ataggtggaa aaaataatca cagcatgtta
      721 ataattatcc acaagttaat cacaatcagt ggataactta aacataacta tgatttatgc
      781 acaccattta tgtatataaa tttaataaat aaatccttgt aaaatcataa tttgaacgac
      841 ttatccacat aattatactt tttaactcca agtgatgtca aatggctgtg aatatgttgt
      901 taaattttga ataactgtgt gtatagcgca tgtgttatcc acaatttttg cgagaaatta
      961 tgtgtataag tggataactt caaataagcg tcacaatttc ttctttttta ctttgggaaa
     1021 taatttatat ggattgcatg aactatgtat atgacaattt attattaaaa ttgcttatat
     1081 taaatcacaa caagtcattt tcaatcgtta tattcgtaaa ttcatcttgc tttataaaag
     1141 gtattttgtt atattgtagt agttgcttga attatgacta gataataata agctatagaa
     1201 caaatttctt aatagcagac acaataagag agcaattgct cagatgaagt atgttagtaa
     1261 ttgaagctaa taaatacttc gttaatttat ctatttatat gaaccaattt ctaggtagga
     1321 agttggaaat attggtaatt gaattcagat ggaggtgttt tgcatggtaa aacgtactta
     1381 tcaaccaaat aaacgtaaac atagtaaagt tcatggtttc agaaaacgca tgagcactaa
     1441 aaatggccgc aaagttttag cgcgtcgtcg tcgtaaaggc cgtaaagttt tatctgcata
     1501 agatcactga atcatcagtg gtcttt
//
LOCUS       DAMJZE010000324         1514 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMJZE010000324 DAMJZE010000000
VERSION     DAMJZE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.333"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            100..507
                     /gene="tnpA"
                     /locus_tag="PC541_003824"
     CDS             100..507
                     /gene="tnpA"
                     /locus_tag="PC541_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2841217.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            509..>1514
                     /locus_tag="PC541_003825"
     CDS             509..>1514
                     /locus_tag="PC541_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2841218.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKVRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTNVDIEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKD
                     L"
BASE COUNT          654 a          156 c          244 g          460 t
ORIGIN      
        1 ttgcgtttct tacgtatgtt atttaaaaat aaatataaaa accttaagaa tacttgtttt
       61 aagtaattac tatgttaata ttatgttggg tgatgaaaaa tggaagtgac tcatggtaga
      121 gggtatgtgt atttagtaca ataccatata gtatggtgtg taaaatatag acatccagta
      181 ttaacaaatg aaattgaaga agatttaatt aatatattaa acaaaatagc tgaagataat
      241 aaatttcaaa tattagaatg taatagtgat aaagatcaca tacatttact aataaattgt
      301 tcaccacaac actatatacc agatataata aaagcattaa aaggtgtatc agcaaggctg
      361 ttaatgaaaa aacatggaga acagttgaaa aagaaattat ggggaggaca tctttggaat
      421 cctagttatt tcgttgctac tgtatcagaa aatacagaag aacagataag aaaatatatt
      481 aataatcaga aaaaacaagt aaagtgaggt ggcagtagtg gaaaaagctt ataagtttag
      541 aatgtatcca aataaaaaac aacaagagtt aattaacaaa acttttggat gttgtagatt
      601 tgtatataat aagtatcttg ctaaaagaat agaagtttat aaaaacaata aagaaacttt
      661 tacttataaa cagtgtagtt ctgatttaac caatttaaaa aaagaattaa aatggcttaa
      721 ggaacctgac aaattttcac tacaaaacgc cttaaaagat ttagataatg catataaaaa
      781 attctttaaa gaaaaagcag gatttcctaa atttaaatca aagaaaatta atagattttc
      841 atataaaact aactttacaa atggaaacat tatgtattgt ggtcaatata taaaactacc
      901 taaacttggt atggttaagg taagggataa acaagtacct caaggtagaa tacttaatgc
      961 tactatatct aaagaaccaa gtggtagata ttatgtatca ttatgctgta ctaatgtaga
     1021 tattgaagta tttgaaaata ctaataatca gataggttta gatttaggaa ttaaagaatt
     1081 ttgtatctca agttgtggag actttataga aaatcctaag tatcttaaga agtcattaaa
     1141 caaacttgct aaattacaaa gagaattatc gagaaagaca atcggtagtt taaatagaaa
     1201 taaagcaaga ttaaaagttg caagacttca agaacatata gctaatcaaa gaaatgattt
     1261 tctacaaaaa ctatctacta aattaataaa agagaatgat attatttgca tagaagattt
     1321 acaagtaaag aatatgatta gaaatcgtaa actatctcgc ttaatttctg atgtgtcatg
     1381 gtcagaattt gttcgtcaat taaaatacaa ggctaattgg catggtagac aaattgtaaa
     1441 ggtaggtaaa ttctttgcaa gttcacaaat atgcaataaa tgtgggtata aaaacgagga
     1501 aatgaaggac ttaa
//
LOCUS       DAMJZE010000325         1484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMJZE010000325 DAMJZE010000000
VERSION     DAMJZE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.335"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            3..611
                     /locus_tag="PC541_003826"
     CDS             3..611
                     /locus_tag="PC541_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2841219.1"
                     /translation="MNDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEVR
                     SKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVKF
                     IIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGSI
                     PNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            909..1247
                     /locus_tag="PC541_003827"
     CDS             909..1247
                     /locus_tag="PC541_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2841220.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          592 a          166 c          191 g          535 t
ORIGIN      
        1 tgataaacga tattaaaaat aaattcaaaa attacaaacc atatataaat ggatacaaga
       61 atatgaagag agcatctgtt ttaattccaa tagtagaaat aaataacaca cactatatat
      121 tatttgaagt tcgctcaaaa aatttgaagc atcaacctag cgaaatttct tttccaggtg
      181 gtaaaataga atcaggtgag actccatatg aagctgttat tagagaaact tgtgaggaac
      241 ttggaacttt tagtgacaat atagaagtaa tatcaagtct tgacctcctt ataacaccag
      301 taaagtttat catacatcca tacttaggat atataaaaaa tattaataat ttaaatataa
      361 acaaggatga agttgaccat acttttttgg ttcctataaa atatctttta gagaatcctc
      421 caaatactta tagtaatact gtaaaagttt ttccagatga aaatctacca tatggttcaa
      481 ttccaaacca aaaagattat aaatttgaaa gtggaagtta tcctattctg ttttataaat
      541 ataaagatta tgtaatttgg ggaatgacag caaaaatact agaaaacttt ttaaattttt
      601 tatcagaata acactcataa taaattatca aaatttttac ataagaaatt tataaaagtt
      661 tctaaattta acaattctaa aatatcaata actatatttt gtagcacttt tttattttat
      721 tattgtttga atatttgatg gttttcaatg caatttcaac ataaatacta tatattagtg
      781 tatactgttg atgaattaaa gtatgataat ctatttaata aagtatgaag taaatgattt
      841 taatgtgcct ttatggtaaa attactaata agtaataaaa ttaattaaaa tatatggagg
      901 tccttataat gtttgctgta ggatttgttg ttataattgc acttgccttt ttcattttaa
      961 gtgtactatt atttaatgga aaagccagta tgctactagc aggttataac tctatgagta
     1021 agaaagaaaa agcacgatac gataaaaaaa aattatgtag aagttctagc attgttactt
     1081 ttattgtctc tataatgcta tttattatgg cgtttttggg atatagaata gagagtggtc
     1141 aaatggacga aaaaaaaatg gttccctttt ctataatatt tataatagtc atactttcca
     1201 gtgtattttt taatatcctc tactctaaca aaaaatgtaa aaaataaata gttgtaagtt
     1261 gagcagttaa ttataaaaga ttgactgctt tttcatgtat attgactact cttattaaat
     1321 attttaacat ttaaaaatct atgtaaaaat aatgaatata gtacaaactt ctaataatga
     1381 ttttaccttt gtggttacag tccactatat ataatttaat atgataacag ggaattatta
     1441 atgacaacta agtaaacttt tataaaatat aataaaacaa aaat
//
LOCUS       DAMJZE010000326         1374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMJZE010000326 DAMJZE010000000
VERSION     DAMJZE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.337"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            207..917
                     /gene="rgaR"
                     /locus_tag="PC541_003828"
     CDS             207..917
                     /gene="rgaR"
                     /locus_tag="PC541_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2841221.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          545 a          112 c          212 g          505 t
ORIGIN      
        1 tattttacta tattttttta cataatatat ctagaaaagt ataggaattt atcgtaattt
       61 aagtttaaat attaaagaga atttctataa gtagtttaat cgacaattta aatttataaa
      121 gaaatttaaa tttgaaagct agtgagtaca gtttggaaat tgaaataaag taaactaaat
      181 gatattttag atttggaggt gatattatgt tcagggtagt tatatgtgat gacgaaaaaa
      241 tacaaagaag catactaaaa gagtttacag aaaaactttt gtctgaaaga tgtctaaatt
      301 atcaaatact ggaattttcc tgtggagaag atttaatatc caaataccca gaaaaaattg
      361 atattatatt tttagatatc caaatgaaag atatcaatgg aattgaggtg gctaaaaaaa
      421 taagaaagtt tgatgaaaaa gtagaaatta tatttacaac ggctttttca gagtatgccc
      481 cccaaggtta tgaggttcgt gcttatagat atttagtaaa gcctatagag tatagtaact
      541 ttgcaatggg tgtaaatctt tgtattgata accttcaaag aaagaaggag cgttatatag
      601 ttttaaatag taaaaaagga tttaatagaa tactgataga ttctatttta tatgttgaaa
      661 ctgtaaagag agatttagtc gtacatacaa taggtagaga ttataaagct aatatgagca
      721 tgaaacaagc agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat acaagtttta
      781 ttgttaattt aagaagaata gaagatctta gggataatat gattactatt aatgaacaat
      841 ttatacctat aagtaagtat agacttaaag atttaaaagt agcattaaca agcttgttat
      901 gtgatataat gtattaattt taaaaaggta attgaaaata tcgtaaaaaa gatttaatgt
      961 catcacaggg ctattgattg aaactgtctt aatatataaa atgttattta tattaagaca
     1021 gttttattat gcaaaatagc agtttataga tttttaatat ggtttaacat gtatatgtag
     1081 aggttccatt atagtagcat gattattaat tagtagcttg attatcaata tactattatt
     1141 gatttttata tgcattatat gtatttttag aatatgatta cctttttaaa ataatgatta
     1201 tttataaaac tctatagata tttgtttcat ttagctacgc atactttaat taattatatt
     1261 aatatttaat ggagtacaat ataaatatta aagcgacatc ttatagttta ttgaaactat
     1321 agtaatgaat taataaatag tttaattcta tgaaaagatt tccttttatg caat
//
LOCUS       DAMJZE010000327         1348 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMJZE010000327 DAMJZE010000000
VERSION     DAMJZE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.338"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(282..1163)
                     /locus_tag="PC541_003829"
     CDS             complement(282..1163)
                     /locus_tag="PC541_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2841222.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
BASE COUNT          464 a          248 c          138 g          498 t
ORIGIN      
        1 aatcaaattt aaacagaata attataccaa tatataaaaa aatttctaaa aaacaataaa
       61 attaagttat aaacaccaat atatttctat tatttcaata agtatacaat tagattagaa
      121 tctaacgcta gtaataaaaa attactttcc caaaatagat ataagcctca ttttatcgtt
      181 caaataatga aaatatattt gatttcatgc aaaaagagtg cctaatgaac taaaaagttc
      241 attttgagac actcattttt ttatataaat ttatatagtt tttaatcgtt cgcattcaac
      301 cactcagtta agacttttat atatttctca ttttcttcaa cgaatggcat atgtcttgaa
      361 tactcaaata attcccactt actattaggt attctatcat acatagtctt tgcaatatat
      421 ggtgaacata aatctattgc tccactagtt actagacaag gctcttttat ttcatgtaat
      481 ctatctgtaa attcatatcc agaaagagtt cctgtaggag aaaattcatt ttgtccccat
      541 cctacaatat atgcttcaga acctgatttt ttaggtcttc tcaggcactc tggagaatct
      601 tctgtcactt cacctgcaca atacatttcc ataaatcttt ctaatgcatc attatattct
      661 ttgctagagt aatctccagt atttactgca tcaagtaatg ctttttggtc aacctcactc
      721 atatatgata ttcttctttt ttgttctttc tcccaaagtt ttgcagaaga aagagtagat
      781 gaaaggatgt aagacttaat accttttggt ttatattcaa tagcatacca aatagcctgc
      841 atacctcccc aagattgacc taacagatga atttcatcaa gtcctagatg ttttcttagt
      901 tcaataagct cctctatcca agtatctgca ttaaatagct caggatgccc ttctacaaat
      961 gaatttccac aaccaatttg gtcatacatt attacttgtc taccactttc agctatctta
     1021 tccaatacct caaaatagtt gtgtgtagaa cctggacctc catgaagtaa tactaatggc
     1081 ttcttccctt ccgtattttc tcctactata cggtaatacg ttttaaaacc tttaaaaggc
     1141 atatatcctt cagtaatttt catgtttatc tcctccaatt tgtaattata aacaataatc
     1201 aagttcatta acaatactat ttaaaacctc ttatacatat ttgattatgt attttaatct
     1261 ttatacctac ttttataaat agtataacat aaaagttaaa aatctctata ctaaaccaat
     1321 tataaaatta tattaactat ataatttt
//
LOCUS       DAMJZE010000328         1343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMJZE010000328 DAMJZE010000000
VERSION     DAMJZE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.339"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            201..1298
                     /gene="ychF"
                     /locus_tag="PC541_003830"
     CDS             201..1298
                     /gene="ychF"
                     /locus_tag="PC541_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2841223.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          524 a          147 c          241 g          431 t
ORIGIN      
        1 tctaaaagca atatttttat ttaaattaaa tacaaatatt atttaaatca atatgcttta
       61 aagtctagtt tacaagcttg atatatatga tgtataatca atatgtatgt ctataattaa
      121 aacaattttg caatcctata gattgattgt ttaaagttat ataaagaata taaaaaagaa
      181 taagagagag gacgattaat atgaaattag gtatagttgg tttaccaaat gtaggtaaaa
      241 gcacactatt taacgcaata actcaagcag gtgcagaatc tgcaaattac cctttttgta
      301 caatagaccc aaatgtggga gttgtatctg ttccagatga aagattaaac aaactacaag
      361 aattatataa ttctgaaaaa atagttccta ctgctataga attttgtgat atagcaggtt
      421 tagttagagg agcttctaaa ggagaaggtt taggaaataa attcttatca catataagag
      481 aagttgatgc aatagttcat gttgttagat gttttgagga tgaaaatgtt gtacatgtag
      541 atggttctgt agacccactt cgtgatatag aaactataaa cttagaatta attttctcag
      601 atatagagat tcttgaaaga agaattaata aaactcaaaa agctgctaag gctgataaaa
      661 ctttaggttc tgaacttgat ttattaaaat caataatgtc tactttagaa gaaagtaaat
      721 gtgtaagaac tatggaattt acagaagatg aacaaacatt cgtaaattca ttggatttac
      781 ttacatctaa gcctgtaata tatgcttcaa atgtatcaga agatgattta gctgataatg
      841 gagaaaataa taaatatgtt caacaagtta aagcatttgc agaaacagaa gatgccgaag
      901 ttgttgtagt atgtgcacaa atagaagcag aaatttctga acttgattct gctgaagaaa
      961 agaaagagtt tttagaaaca ttaggtcttg aacaatcagg gcttgataag cttataaaat
     1021 cttcttatgc tttacttggt ttaatttcat tcttaactgc tggtccaaaa gaagttagag
     1081 catggactat aaaagttgga agtaaagctc ctcaggcagg aggaaaaata cattctgata
     1141 tagaaagagg atttatcaga gcagaaacaa tagcctttaa tgatttggtt gagcatggta
     1201 ctatggctgc tgctaaagaa aaagggttgg ttaaattaga aggtaaagaa tacatagtta
     1261 aagatggaga tgtaatatta tttagattta atgtttaaaa tgattaatcg tatacacttt
     1321 gactattata tataataact aaa
//
LOCUS       DAMJZE010000329         1341 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMJZE010000329 DAMJZE010000000
VERSION     DAMJZE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.340"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(131..1171)
                     /locus_tag="PC541_003831"
     CDS             complement(131..1171)
                     /locus_tag="PC541_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2841224.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          414 a          246 c          134 g          547 t
ORIGIN      
        1 tttaaatttt ttttattttt ggataataaa atgctattac acaaaaaaac acttagaaaa
       61 tagttttcca agtgtttttt atcaatttta taatttcaaa ttttagaatt gagaataaaa
      121 agcctaatta ttatacatct atttttctgt acaatgtagc tagtgatatt ccaagttttt
      181 tggcagctag ttttttagac ttagtatcat ttccgtaaaa ctttaacact ttctttatat
      241 aggctctttc aaactcttca agtgtaagta tgtcttgttc catacttatc tcatagtcac
      301 tttcctcaag taactttata tttatattat ttaacttaaa atattcaagt atctttctat
      361 ttattattcc tttatgtatt attccatctt cctcaagaag gtttaatgcc agttctatag
      421 tattttccaa ctctctaata tttcctggcc aaggatactt aagcaacatt tgttccacat
      481 ctggctctat atggtacacg tttttcttca tctttctcga atatttgtct ataaaattgt
      541 ttattaatat ttgtatatcg tctcctcttt ctctaagtgg aggcacttct aaaggtatta
      601 cattcaatct atagtataag tcttctctaa atttattttc cttaaccaac tcaaataaat
      661 ctctattagt agctgatatg attctaacat ctatatcaat tagttcattt gaacctattc
      721 tcgtaaactt cttttcctgt aataccctca ataactttga ttgtagttga attggcatat
      781 ctcctatctc atctaaaaat acaacccctc cattggccaa ttcaaactta ccaattttac
      841 cttttgaatc tgctccacta aaagctcctt ttgtatatcc aaaaagttca ctttctagta
      901 aaggttctgg tatagcagca cagtttatag caacgaatgt tttattccct ctgtttcctg
      961 cgagatgaat ggctcttgct attaattctt ttccagtgcc actttctcca gttatagcta
     1021 cagtagaatc actatttcca attttacaaa ttctattctt aagagcacgc attacatcag
     1081 actctccaat tatggagtct actgttgttg ttccccaaat attatatact tccattccac
     1141 catacattac tgaatcactg tgtttgtcca taaaatatca actccaaata agtattctat
     1201 ataataatca ttcacattat ctatttaatt atatcataac agaaaagctt agttataatt
     1261 taataatatt attttaaaac aaaacttaat aaaaagtata tctacttagt tttaatttga
     1321 tagatatact ttcttcattt t
//
LOCUS       DAMJZE010000330         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMJZE010000330 DAMJZE010000000
VERSION     DAMJZE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.341"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            27..101
                     /locus_tag="PC541_003832"
     tRNA            27..101
                     /locus_tag="PC541_003832"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC541_003833"
     tRNA            116..200
                     /locus_tag="PC541_003833"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC541_003834"
     tRNA            210..293
                     /locus_tag="PC541_003834"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC541_003835"
     tRNA            323..399
                     /locus_tag="PC541_003835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC541_003836"
     tRNA            407..482
                     /locus_tag="PC541_003836"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC541_003837"
     tRNA            571..659
                     /locus_tag="PC541_003837"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC541_003838"
     tRNA            663..738
                     /locus_tag="PC541_003838"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC541_003839"
     tRNA            745..818
                     /locus_tag="PC541_003839"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC541_003840"
     tRNA            833..909
                     /locus_tag="PC541_003840"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC541_003841"
     tRNA            933..1009
                     /locus_tag="PC541_003841"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC541_003842"
     tRNA            1017..1093
                     /locus_tag="PC541_003842"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC541_003843"
     tRNA            1102..1177
                     /locus_tag="PC541_003843"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC541_003844"
     tRNA            1185..1258
                     /locus_tag="PC541_003844"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC541_003845"
     tRNA            1265..1336
                     /locus_tag="PC541_003845"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMJZE010000331         1301 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMJZE010000331 DAMJZE010000000
VERSION     DAMJZE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.342"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..438
                     /locus_tag="PC541_003846"
     CDS             <1..438
                     /locus_tag="PC541_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2841225.1"
                     /translation="EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKF
                     KKAIRELLWFVCEYLKISGSKSYDYKSVQITFNHSMIINESEKIDMAAKSIGIISDET
                     IVSNHPWVEDVNDELERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            428..>1301
                     /locus_tag="PC541_003847"
     CDS             428..>1301
                     /locus_tag="PC541_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2841226.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGI"
BASE COUNT          551 a          134 c          232 g          384 t
ORIGIN      
        1 gaaaagaata taattatctt tggtcaagga gttaatccag aatctcaaaa cacaggtgac
       61 aaatctggtg tagcacttaa atttttatat tcactacttg atttaaaatg ctccaaaact
      121 gaaaagaagt ttaaaaaagc aattagagaa cttttgtggt ttgtatgtga gtatttaaag
      181 ataagtggta gtaaaagtta tgattataaa tcagttcaaa ttacttttaa ccactcaatg
      241 ataataaatg agtctgaaaa aatagatatg gcagctaaat caattggaat catatcagat
      301 gaaactattg tttccaatca tccttgggtt gaggatgtta atgacgaact tgagagactt
      361 aaaaaacagg aagatactca aaaagagtat gatgatttaa ttcctaataa tcaagatggt
      421 gttatagatg aaacataaag attattggag aaagagattt gaacaattag aagaagctca
      481 aaataacaaa agtgtaaaat attatcttga attagaaaag caatataaac tagctataaa
      541 taatatagaa aaagatatat tagcatggta caacagattt gccaaaaatg aaggaatatc
      601 tttattagaa gctaagaaac tactaaatac aagagaacta gaagagttta aatggagtgt
      661 agaagaatat attaaatatg gtaaagaaaa tgctataaat caaaagtgga tgaaagagtt
      721 agaaaatgct agtgcaagag ttcatataac aaggcttgaa gctttaaagt tacaaataca
      781 gcaacaagta gaagttttat atggaaatga acttgatggt attgataaac taatgagaga
      841 tatttataca agtggatact atcatacagc ttttaatgtt caacaaggag taaacgttgg
      901 ttggagttta atgagtcttg atactaatag aataaataaa attatctcta aaccatgggc
      961 aacagatgga ttaaacttta gtgaaagaat ttggggtaag catagacatg ctttagtaaa
     1021 tgaactacat actaagctaa ctcaatcaat tattagaggt gaaaatccaa agaatctagt
     1081 aaatgacttt gctaaaagat ttaatgtatc taaatctcaa gctaagaact taataatgac
     1141 tgaatcagct ttctttgcat cagcaagtag aaaagattgt tttaatgatt tagatgtaga
     1201 gaaatatgag ataattgcta cactagattt aagaacttca aatatatgca gagaattaga
     1261 tggtaaagta tttgatatga aagattatca agttggaata a
//
LOCUS       DAMJZE010000332         1291 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMJZE010000332 DAMJZE010000000
VERSION     DAMJZE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.343"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>1291
                     /locus_tag="PC541_003848"
     CDS             <1..>1291
                     /locus_tag="PC541_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate acetyltransferase"
                     /protein_id="HDF2841227.1"
                     /translation="RGRIIGDYRRVALYGVDFLMEQKLKDFNTMSTEMSEDVIRLREE
                     LSEQYRSLQDLKELGQKYGFDISRPATNFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLQNGDITEQEVQEIIDHFIMKLRIVKFARTPEYNELFSGDPTWVTESI
                     GGVGIDGRPMVTKNSFRFLHSLDNLGPAPEPNLTVLWSTRLPENFKIYCAKMSIKTSS
                     IQYENDDLMRESYGDDYGIACCVSAMKIGKQMQFFGARANLAKALLYAINGGKDEKSG
                     KQVGPSYEGIKSDVLDYDEVFERYEKMMDWLAGVYINSLNIIHYMHDKYSYERLEMAL
                     HDTEIIRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVTDFEIEGDFPKYGNNDS
                     RVDEIAVDLVERFMTKLRSHKTYRNSEHTMSVLTITS"
BASE COUNT          441 a          218 c          246 g          386 t
ORIGIN      
        1 gacgtggacg tattatcgga gactatcgtc gtgttgcttt atacggtgta gacttcttaa
       61 tggaacaaaa acttaaagac tttaacacaa tgtccactga aatgtctgaa gatgtgattc
      121 gtttacgtga agaattatca gaacaatatc gctcacttca agatttaaaa gaattaggac
      181 aaaaatatgg atttgatatt agccgtcctg ctactaactt caaagaagct gtgcaatggt
      241 tatacttagc atatttagct gctattaaag aacaaaatgg tgcagcaatg agtttaggac
      301 gtacttcaac attcttagat atttatgctg aacgtgattt acaaaatggt gacatcactg
      361 aacaagaagt tcaagaaatc attgaccact tcattatgaa attacgtatc gttaaattcg
      421 cacgtacgcc tgaatataat gaattattct ctggagatcc aacttgggta actgaatcta
      481 tcggtggtgt aggtattgac ggccgtccga tggtaactaa aaactcattc cgtttcttac
      541 actcattaga taatttaggt ccagcaccag aaccaaactt aacagtatta tggtctacac
      601 gcttacctga aaacttcaaa atctattgtg ctaaaatgag tattaaaacg agctcaatcc
      661 aatatgaaaa tgatgactta atgcgtgaaa gctatggcga tgattatggt atcgcttgct
      721 gtgtatctgc catgaagatt ggtaaacaaa tgcaattctt cggtgcacgt gctaacttag
      781 ctaaagcatt actttacgct atcaatggtg gtaaagatga aaaatctggc aaacaagttg
      841 gaccaagtta tgaaggtatt aaatcagacg tactagatta tgatgaagtt ttcgaaagat
      901 atgaaaaaat gatggactgg ttagctggcg tatacatcaa ctcattaaat atcattcact
      961 atatgcatga taaatacagc tatgaacgtc ttgaaatggc tttacatgat acagaaatta
     1021 ttcgcacaat ggcaactggt atcgctggat tgtctgtggc agctgactct ttatcagcga
     1081 ttaaatatgc acaagttaaa cctatccgta acgaagaagg tcttgtaact gactttgaaa
     1141 tcgaaggcga cttccctaaa tatggtaata atgacagtcg tgttgatgaa attgcagtag
     1201 atttagttga acgtttcatg actaaattac gtagccataa aacataccgt aattctgaac
     1261 acacaatgag tgtattaaca attacttcaa a
//
LOCUS       DAMJZE010000333         1283 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMJZE010000333 DAMJZE010000000
VERSION     DAMJZE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.344"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(163..429)
                     /locus_tag="PC541_003849"
     CDS             complement(163..429)
                     /locus_tag="PC541_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2841228.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(471..1280)
                     /locus_tag="PC541_003850"
     CDS             complement(471..1280)
                     /locus_tag="PC541_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841229.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          444 a          185 c          149 g          505 t
ORIGIN      
        1 tttatatttc caattttagt aatcagattt tatcaataaa ttactattct attaatttaa
       61 ttaaataacc tttaaattta attataaaaa ctgttgcaga aatttctaca acagtttaaa
      121 aaatttacct tacaaaaact tattttataa caagagagta tattataagg ctagtacagc
      181 ccattttttg cactgagaaa ttcttttgcc atcatcacct tgttctttgt catatgcaaa
      241 ttgattaagc atttcacatg gaaatgtatc acatagacca caatgtgtaa ggtttttatc
      301 ttcacaacaa gattttacag gacattgttc accccaaaat ggtttctcaa tctctgtaca
      361 acctttacaa ttgacttttt ccttgtatcc acattcataa cataaaattc cacatcttga
      421 ttctatcatt ttaagtcact ccttaaatat aaattttata tgattgtata tcaaaattta
      481 tacttttcat tgtaaaaatc ggacaaattt tttatatagg ttgttggatt aacaccacaa
      541 atagatttaa aatcatttat aaaatgagat tggtcgtaat aaccaatatc ttgtgctaaa
      601 gttgcaaatg gtattttatt attttgtatt tcttgtaata caaggtttat acgaagtaat
      661 ctaaggtatg atttaacact cataccaaga gaattattaa aaattctatt taaatgtcgt
      721 tcactataac aactaatttg tgaaatgttt ttaacagaca taatagagtt ttgttttcta
      781 gcattttcaa gaattggtat agtcatatct actatatttg atttcaataa gtatgataag
      841 aataacatat ctaattgttc tactaattcc tttatagtac ttgttctttc aaaaatggaa
      901 ttaacctcta agctaaataa tgtgttaaaa tcttcaagtg aaatttttaa atctgtagat
      961 tctctttgag aaagacctgt caaataatat actccacctg ggcgaaactc aacgaatact
     1021 ctaaaaagta cattttctat atcatttttt acaatagtag tttttgttgt ggctccccag
     1081 aatgcgcttt ctataccttt tttgtcaaat ttaaatatca tacatccact agcatcaggt
     1141 attaatgtaa gattttcttt aatacttgta tctggaatag atattgtata gtgtgctata
     1201 tactgtctta acaatttatg tggcaaaata tatgtatact gtttgcaggt atttaaaatg
     1261 ttattgatta ttttattcat aag
//
LOCUS       DAMJZE010000334         1210 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMJZE010000334 DAMJZE010000000
VERSION     DAMJZE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.346"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            61..756
                     /gene="cas6"
                     /locus_tag="PC541_003851"
     CDS             61..756
                     /gene="cas6"
                     /locus_tag="PC541_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2841230.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            747..>1210
                     /locus_tag="PC541_003852"
     CDS             747..>1210
                     /locus_tag="PC541_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2841231.1"
                     /translation="MVIGGENLLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKN
                     FSNIDILEVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKK
                     ENLRPDKNNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKV"
BASE COUNT          524 a           93 c          189 g          404 t
ORIGIN      
        1 tgttattaaa tagatgaaaa aatcctaata tggattatca gaaaatgagg aggttgttaa
       61 ttgataaaat gtcttgaaat gtctgtaaaa gtgtatcttc ttgaaaatat ttctttagat
      121 aatacatcaa aagaaatacg taaattaata gatgatacat taattataga agaaaaatat
      181 aaggaatttc ataataaagc tggatttaag aattattctt ttaatttatt atacccatta
      241 gaaaaagata aggtttataa gaaggattgt atatatactt ttattattag aactgtagat
      301 agtgaattag gaatgtattt tatgaataat ttaaaaaatc agcattctga taaaataaag
      361 gttattacaa tgagtaaaaa agtgatccct aaaaaacata tagaaagtat ttactctgtc
      421 actccaataa tagctaaatt tgaggatggg tattggagaa gtaataaaac agtagaagat
      481 ttagaaaaaa gaatcagaga aaatttaata aaaaaatata atgaatttac taacagtaaa
      541 atagatgaag attttgaatt atttcaagtt attaaattta taaataggaa acctatttct
      601 actaaatata agaatatatc tttacttggt gataaaataa gttttaaagt atcagaaaat
      661 gatatggctc aggaaattgc aaattttgca cttggagttg gagtcggaga aattaattct
      721 cgtggatttg gttttgtaaa ctatagatgg ttataggagg tgaaaacttg ttaaaggatt
      781 gtttagagat ttttgaaaaa gaatacaaat ctttaggaaa tagattgata actgatagct
      841 atgtcctatc ctatggaaca tatatattgg tgaaaaattt ttcgaatata gatatattag
      901 aagtttctaa aaataatgtg gatagtacag aagatatata ccataaattt tgtgaactgg
      961 attatttatc aaaactggtg tctatagata aatcagttga ttccaaaaaa ataatacata
     1021 gtaataatta tctttcattt tttgtaaaaa aggaaaactt aagacctgat aaaaataata
     1081 atgtaaaact tacagaagaa attattgata attattatga tgctttgtta aacccaagaa
     1141 ttaaatataa atctgataaa agaaaaaaag aaatctatga ttcaatagaa aataaatatg
     1201 gtaaagtgga
//
LOCUS       DAMJZE010000335         1198 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMJZE010000335 DAMJZE010000000
VERSION     DAMJZE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.347"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(111..923)
                     /locus_tag="PC541_003853"
     CDS             complement(111..923)
                     /locus_tag="PC541_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2841232.1"
                     /translation="MYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
BASE COUNT          428 a          195 c           98 g          477 t
ORIGIN      
        1 aatgtactaa aaataacata aaagtttcca tttttcaata tgtaagcttt tcttatgtta
       61 ttttattgtt aaaggtttca agtttttatt tattcatttt aaaaaaatta ttatttttgt
      121 aaaactaatc ccatagtcat actatcgtca gaatatgctt cacccttaac attactaaaa
      181 aactcaacag acttaaatcc aatattttca acttctaata agaaactttc ttttgaaaaa
      241 tagtgattcc aaacataaaa tacattttca tctttttcgg ttattacaag agtttgttct
      301 aatgtattat aatcattgta tttacaatta tcttgtagac acatatattt ttcattactc
      361 caaaatcctc catcttcatt tatttcccaa tatttaattt cctcaaaatt attgtattta
      421 tttattgtaa atacatctaa tattaatttt cctccaggtt ttaaactatc ataaattttt
      481 tccattaata accttctatt ttcagttgac aaagctccat aatcacaata aattaaggta
      541 gccaaatcaa attcctcatt atagttcata tttaaataac tttgaaacaa gtaatttata
      601 tttaaattag tctccttatt tctattttgt gcatagttaa tagaacgttt tgagaaatca
      661 attccagtta ccttataacc tttttgtgca aatttctctg catataaacc tggaccacaa
      721 cctaaatcaa gtaatttagg ataattaact ggatttgcaa ctgtaactat ccaatttaca
      781 gaatcctcga taaagttaaa atttctactt gctccttcaa aattagtgtc taagtgtgct
      841 tttaataatt gctctgaaat atactcatca tcccaaaaat taatttcact ttgtttataa
      901 agctctggtc tttctaaata catacataat ttgtttaaca aaacatacca ctccttaaaa
      961 tttattttta aaataaaaaa accacagagt ttactctgtg gtttaacttc aaaaagatga
     1021 gtttaaataa gagtaaaata gaaccaaata tttttaagca caacaataaa actatagctt
     1081 tactttgttt gcttattaaa tttgatttta ttcactcttt tccagcaata cttatatcaa
     1141 cgattttatg atatcaaata tcaccgaaca acatcatcct ttcatatttt tattttat
//
LOCUS       DAMJZE010000336         1186 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMJZE010000336 DAMJZE010000000
VERSION     DAMJZE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.348"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            64..1104
                     /locus_tag="PC541_003854"
     CDS             64..1104
                     /locus_tag="PC541_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2841233.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          462 a          141 c          194 g          389 t
ORIGIN      
        1 ttttcagaac atttgtattt atgttataat ttgtataaat atatttattt aggaggaaac
       61 aatatgaatt taaatttaac taatactata aatttaatga aaaaatattt ggacattcca
      121 agcccagctg gatatactga aaatgcagtt ttagaaatca aaaaagattt tgaaaattta
      181 agtcttgaca ctattcttac aaaaaaaggt gcacttatag ctactcttcc tggtgaagat
      241 gattcaaatc aaataacaat atctgcacat atggatacac ttggagctat agtaaaagaa
      301 atatcatctg atggtactct taaataccat aaagttggtg gtggatgttg ggcggctata
      361 gagggagaaa actgtactgt tataactaga aaaggtaaaa aaatcagagg aagtgttgta
      421 tttaagtatg cttctactca tatatatggt caaacaaaag ctgccactga aagaaatgaa
      481 gaaacaatga ttattagact tgatgaaaaa gtatttacaa agcaagatgt attagattta
      541 ggaataagtg ttggggattt tgtatgccta gaccatagat ttgaggctac agaatctggt
      601 tttataaaat ctagatatat agataataaa tcagctgtag caatggttct tgaaatatgt
      661 agatatttta aagaaaacaa tttaacacct aagcatacta ctaactttta tataagtaat
      721 tatgaagaaa ttggacatgg tgtttctaaa tgtttacctg aaaaaacaaa agaatttgta
      781 gcaatagata tagcacctac tggaccaagt caaacatcta gtgaacatgg tgtaactata
      841 gttgcaaaag ataatttatc attatatgat tttaatttaa gaaacaagct tgtcgatgtt
      901 gcagaagaca ataatttaga ctacgctgtc gatgtattta ctttctatgg ttcagatgct
      961 tcagaagcta ttcgttgggg agaagatgtt caaatagcat gtgttggtcc tggaacaaac
     1021 aatagtcacc attatgaaag aacacatata gaagctattg aaaatacact aaaattactt
     1081 ataaattata tgcttaaaga ataattaatt atatatttta ccaaagtcaa ttcttaacaa
     1141 ataattaact ttaattttta tttttataca aaaattggtt tttcta
//
LOCUS       DAMJZE010000337         1183 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMJZE010000337 DAMJZE010000000
VERSION     DAMJZE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.349"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            73..1149
                     /locus_tag="PC541_003855"
     CDS             73..1149
                     /locus_tag="PC541_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2841234.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          459 a          170 c          212 g          342 t
ORIGIN      
        1 atattatagt tattaaaaat aaggacagta aaaacgctat ccatattaca aactaaaaag
       61 gggggaatta taatgagtaa gaaattattt attcctggtc ctattgatgt taaagaagaa
      121 gtcttgcaaa agatggctac accaatgata ggacatagag gaaaagatgc atctatgctc
      181 caaaagagta taagtgaaaa gatgcaaaaa ctattctata caaacaacac gatactttta
      241 tccacgtcat ccggaactgg tttaatggaa ggctccattc gttcctgtac atccaaaaag
      301 gcagcagtat tttcctgtgg atcctttggt gatagatggt acaaaatggc agtggctaat
      361 aatgtcccag cggatatatt taaagttgaa ttaggagagg caacaacgcc agaaatggtt
      421 gataaggtgc tttctaccgg agaatacgat ttaataactg taactcacaa tgaaacttca
      481 actggaataa gaaatccaat agaagaaatt ggagaagttg ttaaaaagta tgaggatgta
      541 atctactgtg tagatactgt aagttcagcc ggtggaataa aagtcgaggt tgataagatt
      601 ggaatagata tttgtataac atcagtacaa aaagcactgg gattaccacc aggaatgtct
      661 atttgcacat tttctcaaaa agcaatcgat agagctaagc aagtaccttt tagaggagta
      721 tatcttgatt tgttagcaat gtatgaatac ttaatcaaga aaaactacca atacccttca
      781 acaccatctc tttctcacat gttcgcttta gatttccaat tagataatat attagatgaa
      841 ggcctagata acagattcaa cagacatgaa gacatggcaa acttagttag aaactgggct
      901 aaaaaacact tccaaatttt taccaatgaa aatcacttat ccaatacact tactgttata
      961 gaaaatactc aaggtattag tgtatcaaat ttaaattcaa aattacaaga acgaggattc
     1021 caaatagcaa atggctatgg agatttaaaa gaaaaaactt ttagaatttc acatatgggt
     1081 gactatactg tcgaagatgt acaagaatta ttagataata ttgatgatat attaggtttt
     1141 aataaataat attgtaaaaa tatgtatgtt ttaagataaa ttt
//
LOCUS       DAMJZE010000338         1172 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMJZE010000338 DAMJZE010000000
VERSION     DAMJZE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.350"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..464)
                     /locus_tag="PC541_003856"
     CDS             complement(<1..464)
                     /locus_tag="PC541_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2841235.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDY"
     gene            complement(464..895)
                     /locus_tag="PC541_003857"
     CDS             complement(464..895)
                     /locus_tag="PC541_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2841236.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQNWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKKLKDRIGNLLDTMTDELNIKGGSK"
     gene            complement(957..1139)
                     /locus_tag="PC541_003858"
     CDS             complement(957..1139)
                     /locus_tag="PC541_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841237.1"
                     /translation="MNINNIVVRILAERILSGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIEHSGVVEGTEPTK"
BASE COUNT          370 a          216 c          114 g          472 t
ORIGIN      
        1 tcataatctt ctttattatc acagaagaaa ctttcaatta atactgctgt aggctttgaa
       61 ctatttaaga tatataatct tttatctaat ttagcacctc tatttttaaa tactgtacct
      121 agtttatcac atattctagt tgcatactct aagcctttat tactataata taggacttct
      181 gaacctttac cttgaccgtt acttgcattt aaatgtaact ctataagtaa atcatatcct
      241 ccactattaa ctctaggtat tttataagac ttttcttcat tcttagtttt aaactgcttt
      301 tctgggcata ttattacatc taccttatgc ccttcttttc taaatgtatc tgctaatact
      361 ggtgcaagag atttgttgta ttgatactcg ttaattactc catcagcaga agtgcatgct
      421 ccacttttta aaatactgtg tcctactgta atacatattt tcattattta ctacctcctt
      481 taatatttaa ctcatctgtc atagtatcta ataagttacc tattctatct tttaattttt
      541 taggcactgg taacccacac aaatacatgt tttttaatat acttacactt tcatatagaa
      601 taaataaaat agagaaaaat tcagatattc caagatgatt taatcgtaaa aaatcaaccc
      661 aattctgcgg taacataaat aaaaaattaa actttgtaag tatgtcaact actgctagaa
      721 aaaatataca tgctatcatt gcaacttttc ttattcctcc atttattcca aaagagctat
      781 taaactgatg tgtttttatt gcccttaagc aacctagtaa tgtatcaaat gctattgcta
      841 atattactaa ttttataaat atatttgttg ctaaaaaaac tattgttacg ttcatatttc
      901 ctccttattt tgcattaaaa taagacttag aattatctaa gcctataaaa aagaacctat
      961 tttgtaggtt ctgttccttc tactactcca ctatgctcta taatataatc ctctactgct
     1021 tttctatact ctgtgttagt tacgtcatct aattcaaatt ctcgattttt tagagggttt
     1081 aaacctccac ttaaaatcct ctctgctaat attcttacta caatattatt tatgttcatt
     1141 ataaaattcc tcctactttt tcgttttctg ca
//
LOCUS       DAMJZE010000339         1167 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMJZE010000339 DAMJZE010000000
VERSION     DAMJZE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.351"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..203)
                     /locus_tag="PC541_003859"
     CDS             complement(<1..203)
                     /locus_tag="PC541_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2841238.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKY"
     gene            complement(204..380)
                     /locus_tag="PC541_003860"
     CDS             complement(204..380)
                     /locus_tag="PC541_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841239.1"
                     /translation="MNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEEIINKFMKGVS"
     gene            complement(373..810)
                     /locus_tag="PC541_003861"
     CDS             complement(373..810)
                     /locus_tag="PC541_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841240.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(803..>1167)
                     /locus_tag="PC541_003862"
     CDS             complement(803..>1167)
                     /locus_tag="PC541_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2841241.1"
                     /translation="LTKIDLDKFCKDAARELAARLLGKVIRRTPVDTGFLRQGWNGVA
                     YARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDGKGWVKGQHFLTISEMELQSQV
                     DKIIEKKLLILLKGVFDA"
BASE COUNT          356 a          242 c          119 g          450 t
ORIGIN      
        1 aaatacttca ctgaatactt ctcaaaatca tcactggtta cttgaaatac gtcttcatct
       61 atgccccaat ctaactctaa aggtattgca acaatacctc tatccgataa tgaactggtt
      121 gccctcttag ctgagataaa atttatatag ctacctggta atattttatt ctgtgttaca
      181 aatgttcctc cacctaacgc catctagctc actcctttca taaatttatt tattatttcc
      241 tctacctctg agaaggaata taactcattt tcttttaaaa ttgcatttaa taagtctttt
      301 ctatttacat acttcttaga attaactatt tgctccttag taaacttgta gtcatcttct
      361 ttgcttaatg ttttattcaa aattatcacc tctcttcaaa ccaccgaata actctactgt
      421 atccatctta ttggtatcat tattttttat agtaaaatag ttataatcaa caaagaagtg
      481 aagaacattg tctataattt caaaattcat atttgtacct ctgactaaat ctccatcaat
      541 ttctatatac tctaattcct ccagtagcat ctcagctatc tcatttattt caaaattctt
      601 agcttctgaa cgagggaaat aatgtacatc aaaagaattt ttctttaatt ctctcccgct
      661 tggatatggt gtcttgcttg gatttaaagg aacaataaaa aaacaaggtt cattaatacc
      721 ttgctctaca tcctcactat aaattgtata tttttctcca aatgatttat ctaactttac
      781 tgatattcca tctataatat tattaagcat caaatactcc tttaagtaat attaataact
      841 ttttctctat aatcttatca acttggcttt gtagttccat ctctgaaatt gttaagaaat
      901 gttgtccttt aacccaacct tttccatctt tagttctatg cccgaaattt acataacttg
      961 catattcagt cggattaaca acctctataa tataattatt tccttgttta tacacaggaa
     1021 gcgacctagc ataagccact ccattccatc cttgtcttaa gaatcctgta tcaactggtg
     1081 tccttctaat tacttttcct aagagtcttg ctgctaattc tcttgctgca tctttgcaaa
     1141 acttatctaa atcaatcttt gtaagct
//
LOCUS       DAMJZE010000340         1167 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMJZE010000340 DAMJZE010000000
VERSION     DAMJZE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.352"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..203)
                     /locus_tag="PC541_003863"
     CDS             complement(<1..203)
                     /locus_tag="PC541_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2841242.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDKDVFQVTSDDFEKYSVKY"
     gene            complement(204..380)
                     /locus_tag="PC541_003864"
     CDS             complement(204..380)
                     /locus_tag="PC541_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841243.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            complement(373..810)
                     /locus_tag="PC541_003865"
     CDS             complement(373..810)
                     /locus_tag="PC541_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841244.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(803..>1167)
                     /locus_tag="PC541_003866"
     CDS             complement(803..>1167)
                     /locus_tag="PC541_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2841245.1"
                     /translation="LTKIDLDKFCKDAARELAARLLGKVIRRTPVDTGFLRQGWNGVA
                     YARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDGKGWVKGQHFLTISEMELQSQV
                     DKIIEKKLLILLKGVFDA"
BASE COUNT          362 a          242 c          120 g          443 t
ORIGIN      
        1 aaatacttca ctgaatactt ctcaaaatca tcactggtta cttgaaatac gtctttatct
       61 atgccccaat ctaactctaa aggtattgca acaatacctc tatccgataa tgaactggtt
      121 gcccttgtag cacttacaaa atttatatat gcaccaggta ggaccttatt ttgtgttaca
      181 aatgttcctc cacctaaagc catctaactc actcctttca taaatttatt tattctatcc
      241 tctacctctg agaaggaata taaatcattt tcttttaaaa ttgcatttaa taaatctttt
      301 ctatttacat acttcttaga gttaactatc tgctccttag taaacttgta gttatcttct
      361 ttgcttaatg ttttactcaa aattatcacc tctcttcaaa ccaccgaata actctactgt
      421 atccatctta tctgtgtcat tactttttat agtaaagtag ttataatcaa caaagaaatg
      481 aagaacatta tctataattt caaagttcat atttgtacct ctgactaagt ctccatcaat
      541 ttctatatac tctaattcct ccagtaacat ctcagctatc tcatttattt caaatgattt
      601 atcattactt ttaggaaaat aatgtacatc aaaagagttc ttttttaatg tcctgccact
      661 tagataggat actttgcttg gatttaaagg aacaataaaa aaacaaggtt catttatacc
      721 ttgctccaca tcttcactat aaattgtata actctctcca aatgttttat ctaatttaat
      781 agatattcca tcaattatat tattaagcat caaatactcc tttaagcaat attaatagtt
      841 ttttctctat aatcttatca acttggcttt gtagttccat ctctgaaatt gttaagaaat
      901 gttgtccttt aacccaacct tttccatctt tagttctatg gccatattca acataacttg
      961 catatgtggt cggattaaca acctctataa tataattatt tccttgttta tacacaggaa
     1021 gcgacctagc ataagccact ccattccatc cttgtcgtaa gaatcctgta tcaactggtg
     1081 ttcttctaat tactttccca agtaatcgtg ctgctaattc tcttgctgca tccttgcaaa
     1141 acttatctaa atcaatcttt gtaagct
//
LOCUS       DAMJZE010000341         1150 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMJZE010000341 DAMJZE010000000
VERSION     DAMJZE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.353"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            37..294
                     /locus_tag="PC541_003867"
     CDS             37..294
                     /locus_tag="PC541_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841246.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(404..1084)
                     /locus_tag="PC541_003868"
     CDS             complement(404..1084)
                     /locus_tag="PC541_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2841247.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          470 a          145 c          132 g          403 t
ORIGIN      
        1 gaaattaaaa tgaattaagt aaatggagga attgatatga taataattag aagtcaagat
       61 aaaactaatt taatgcagat aaaccaaatt aaaatagatg gtagtcaagt ttatgcagta
      121 tttgaaagta aaattgatac tgtaaaaata ggagattatg aaaataatac aagagctata
      181 caagtgttgg acaatataca aaacttcatt gaaaatggaa caaaatatga ttatataaca
      241 tcaaataaag tcagatacaa tgttaataaa atatttcaga tgccagctaa atagtgagtg
      301 tgcattttaa tttaataact aaagcccact ttcttttgta gtatgaaaga gggctttaca
      361 tatttgtaaa tatcgaagta cacttatttt atcaataaat tatttaataa ggtataatgg
      421 accaattaaa caataaaaat tctcctaaaa cagcaagtat taaatatata atagcttcat
      481 ttttgtaaag agaggtaatt tttttgctta tgtcatcatc aattgtaata gcaatctcat
      541 cttgaataat atttttatat atagatggga aaatatcttt tttctttata agattaaata
      601 aattatttac acctacccat ataaaatatc ctaaaacagc acctaatgtg ttcatcatta
      661 aatcatcaat atctgtagct cttaagttaa acaattgaca aagttcaatt gctaatgaaa
      721 aacctaatcc agttaatgaa acattgataa tatttctaaa atttttccaa attaaaggta
      781 gtaaaaatcc taaaggcata aacataatta tatttaaaat atatgttaag atgccaactg
      841 agtcaaaagg tattagattt atttcatcaa ttctaaaaag cccactatac cttcctatat
      901 cccatatgct accaattcca gctttactaa gtgctagata gatatataat aaaaaaatat
      961 atacccatac taaatgtatc attacattct tttcaacttt caaatttttt cgttttataa
     1021 cgactatttg gtatattaag catggtaata taacgcaaat aaatgaataa aatgtccata
     1081 tcattttatt ttgtcccctt tcaaatttat gtttttagtt ttataagtac tatctttata
     1141 ttaaactgta
//
LOCUS       DAMJZE010000342         1137 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMJZE010000342 DAMJZE010000000
VERSION     DAMJZE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.354"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            55..720
                     /locus_tag="PC541_003869"
     CDS             55..720
                     /locus_tag="PC541_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841248.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT          465 a          126 c          146 g          400 t
ORIGIN      
        1 atataagttt tataaagtat attaaatata ttataagatg gaggtcgagt aattttgaaa
       61 ggaaacatag tacaatataa ttttgcagat atagaggaag aagtatattc gttggattat
      121 gctatagcat ggaatacaga cgaggaaaac gtaaacataa taccatttac aaataaattc
      181 tgtaaagaat ctatagaatc tttttgccta ggtaaaatta ataactttgt tgaaatatta
      241 aatgaaggtt ttgttgaaaa tcatcattat gtacatttag ataaaatgat tagtgttcct
      301 aagaaaaagg ttaatttagt atatcaacaa gacacacatg gttatcttct aagagatgac
      361 aatgacaacc ttattcctgc aaaaataact tcagagcaat ctaaatctat atcaagtaag
      421 atggagttat tctgtgctgg tgaagaaaaa tgtttaatta atatattact aaaggctgac
      481 ccatcatata ttttagatgt agatagtata aaagacaaaa acattctaaa tctaggttat
      541 gagtctattg acagatataa agaatacaat tttgatgatg ataagatttt aatatttttt
      601 ataaataaaa aacgttattc agtgattatg aaaaaaacaa acaactctga taacgattta
      661 gtttccagaa acaatgctat taaagaatta tttactaata aagcaggaaa tcttaactaa
      721 taatatagtt ttaaattatg ttattgatta aattaataat agtatacaaa gaggctgtct
      781 caaaatagag ataagcttct ttttcattgt tgaaataata aaatatattt aattctatat
      841 aaaagagagt gcctcataaa ctaaaaagtt cattttgaga caccctccta tttataataa
      901 acaaataaat ccttaaacct attgagtgat tataattttt agattgggtt atcttgcatt
      961 tttaaattta aggtaacttt ctactctaaa ctttaatctc taatcaatat catcaactgt
     1021 aacaacttac aattaaatct ttaataatca aactttagac tatctatatt aaaactcaaa
     1081 ttagatttag gatttatttg gatatcacat ttattttatt aactagtaat caattat
//
LOCUS       DAMJZE010000343         1133 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMJZE010000343 DAMJZE010000000
VERSION     DAMJZE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.355"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            231..371
                     /locus_tag="PC541_003870"
     CDS             231..371
                     /locus_tag="PC541_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841249.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(368..739)
                     /locus_tag="PC541_003871"
     CDS             complement(368..739)
                     /locus_tag="PC541_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841250.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          440 a          116 c          135 g          442 t
ORIGIN      
        1 aaaacataca agattctaca ataatagatt tttttgttat tattacgagt tttttattgc
       61 aactgtagaa taaaagtatt attatataat taagataatt atctagggaa attttggata
      121 atcaaaatat attttataaa atcaaaatac aattacataa actttattaa aaatttgttg
      181 ggaatttctg actaacatta agaacgtaag tttgttgggg gatggtatat gtggaaaagt
      241 caaacactac tgttacaaaa aaagaaaaaa ttgaagatat aggaatgaaa ttagaacatt
      301 tattgagtga agatattact ttttatgaat tattacttga gattttgtat aaagaaaaag
      361 aagagaatta attctcttct ttaaggaatc tttttaaata ggcattatca atcatttttt
      421 ctatagcttc tctatcttct ttttcaagag aatatatttt ttctaagagc ttttttgttt
      481 tttcacttac tgataagttc tctatgatta atttatcttc tttgcttatt tttgtattat
      541 catttacgcc taataaataa tctgttgatg tttctaatgc ttttgctaat ctaactatta
      601 tttcactttt aggttctctt aaattatttt cataacgtga caagctagcc tcagttatac
      661 cgactttagt agcaagttct ttttgattta tatctaattc ttttcgtaga tttgctattc
      721 tatctcctaa actttccata tgtacatcac tccttaccga aattataagt tattttgata
      781 aaaagtaaaa taaaattacc aaaaatataa aaaaaaaagg ttaaaataat aaaatatatt
      841 gaaaattacc atttgtatat gtataatatg aattatacaa atggtaattt gatgagaggt
      901 ggtatgttga aataagaaac tgaattaatt aataaataca tagaaagttt tgaaaacctt
      961 taattgagat ataaacaata tgtacaaaat tttttgcaag taaaattacc aaatgtataa
     1021 aataaatcag tagcatagat aattttatgt tttaatacat tagattattt aatttagtta
     1081 taatggtcaa aaatgcaatc tacaaataaa acaaagtatt atcaaaaaag tat
//
LOCUS       DAMJZE010000344         1118 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMJZE010000344 DAMJZE010000000
VERSION     DAMJZE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.356"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..640
                     /locus_tag="PC541_003872"
     CDS             <1..640
                     /locus_tag="PC541_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDF2841251.1"
                     /translation="CRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYNEWYEKHIEG
                     RLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYNDTIRYEEAKK
                     LYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGNTGKPRLDLD
                     LTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDILKGE"
     gene            642..890
                     /locus_tag="PC541_003873"
     CDS             642..890
                     /locus_tag="PC541_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841252.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            960..>1118
                     /locus_tag="PC541_003874"
     CDS             960..>1118
                     /locus_tag="PC541_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDF2841253.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANE"
BASE COUNT          501 a          107 c          201 g          309 t
ORIGIN      
        1 ttgtaggaca acaacagctc cttggtttga agatgaagaa ggttacagag cagcaaaagg
       61 agaagatgga aaaacatatt atgtaccatc taatatgaag tataatgagt ggtatgagaa
      121 acatatagag ggtagacttg gaaaagaaaa agctgatacc cttagaaaaa tgcaactaaa
      181 tgagagtaaa gatagaaaac aatttgaaga atataaaaag gtattaggaa atgaaatacc
      241 ttctaaattt gatgaatatc aacatatgaa gtataatgat actataaggt atgaggaagc
      301 taaaaagctt tataaggatg taaattggca agttaagaat cagagaaatt taactagtgg
      361 aagtgttcat tcagtgccat ttgaatcaaa accaaatagt gtatttgata attacaaaga
      421 tggtaaatta attcaaagaa gatactatgg taatacagga aaacctagat tagatttaga
      481 cttaactgac catagaaacc caaagcaaca taaaattgta cctcataaac atgattggtt
      541 agctgatgaa aataaccatg ataaggtaaa aagagagaag gatatggagc ttacaaaagc
      601 tcataaaata gctaacaaag atattttgaa aggagagtaa aatggataat aaatttaaga
      661 atttacaaca tttaattgat tctattgatt taggattaga tatagaattt gacttatata
      721 ataaaccata taatatatct attggagatg atgatacaag gtttattact ctttgcccta
      781 atggagatac aaagtattat aaaaatggta aggatatggt tgataattac aacattgatg
      841 gtaaattact aaaggattta tggaaagata tacaaatagt aaatatgtaa aagcacttgc
      901 taaatgataa attagtaggt gcttttatta tgtaaaaaat gaaaggagat atttaaaaga
      961 tggattggtt aaaagaattg ctagaaggaa taaaaataga aaataacaaa attgatgtag
     1021 tttctcttca aaaatctata gaaaagaaaa taaaagagac tacaattact caagaagatt
     1081 atacaaatct tgaaacacaa cttaatacag ctaatgaa
//
LOCUS       DAMJZE010000345         1106 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMJZE010000345 DAMJZE010000000
VERSION     DAMJZE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.357"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(221..1090)
                     /locus_tag="PC541_003875"
     CDS             complement(221..1090)
                     /locus_tag="PC541_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2841254.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          418 a          171 c          104 g          413 t
ORIGIN      
        1 aaataataat ctaaaataca tcaaaaaata aactaaaaaa tatcgaaaaa taatttaaaa
       61 gacatcaaaa ctaaatcttc taatcagcaa ttaaaagtct attttcttag tttaaaaaat
      121 aaagatagat tttttcaaaa cactggataa ataggtaaaa aaataaacag ttagtaaaat
      181 aaatttttac taactgttta tttttattgt ttaatatgtc ctataaatcc tctaagtaat
      241 attttccttg caaaaattct gattctaaac ctagattttt aaaataatca accatgccca
      301 tacacgcaaa tttttctgta gaataatgag tagcacctat aacattaatt aaattatctt
      361 ttgctatttg atggaactct aatactggtt caaaatgttt taaaggctta gtaaacccag
      421 taatatatag atttatacca agttcagcaa cttctcttgc tacaaatgga taacttcctc
      481 cccctgctgc aatagcaact aaaccatctt ttaatacaga atctccataa tcatacaatt
      541 tcacatcatg accaacagat tttttaatta attccttaat ctcttctatt gattttagtg
      601 atgttttaca tataactcct acctcaattt tatcgtcata tttacaaaat ggtttgacta
      661 tttctaaacc catagctttt gcaaaactaa cagaagttga atagtcacta tagttgtcta
      721 gtggagaatg taatacataa aaagaaattc tacgattttt catctcttcc atataagata
      781 gtggtatgtc gtgaaatgga aaaccttcat ctgatgctat gtatcccatt gcatgatgtg
      841 aaaaaattaa aatatctttt tcatctttat ctataacttg cttaattatt tctttatcag
      901 gaaaagtagt tgtgtatact ttattaatat tttgagcatt atctaatact aatcctatgt
      961 atttttttct aaattctgga gtaataaaat cattttcaaa attcataaaa ctccaatcat
     1021 cattaatatt tttaatatta aattctaaat ctaatttttt ataaagttca attgccttaa
     1081 taataaccac tcctttataa taatat
//
LOCUS       DAMJZE010000346         1094 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMJZE010000346 DAMJZE010000000
VERSION     DAMJZE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.358"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(212..922)
                     /locus_tag="PC541_003876"
     CDS             complement(212..922)
                     /locus_tag="PC541_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2841255.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          388 a          145 c          126 g          435 t
ORIGIN      
        1 ttttgaaaga ttgaaattct agaaactgag ctttaaaaaa ttaagtttga aaaaaactct
       61 aaaaactaag cttcaaaaaa ttaaattttg aaagattgaa attctagaaa ttaagcttca
      121 aaaaattaaa tttgaaaaaa caaaactcta aaaactaagc tttaaaaagt taagtttcaa
      181 aaataaaatt ctagagattg aacttcaaaa attaagattc taacatatac cttgtaaatt
      241 ttcctacaac ctcttgatat tttttacggc ttataggaat tttttcattc tgaactttgg
      301 cataagtttg agttatacta tctacatggt ttagattgac aatatacctt tgatgcgtac
      361 gtataaaatt agagggaaga acagtttcca tatcatttaa ttttccgtag tattctaatg
      421 ttttatcaaa ggtaactaca gttaattttc ttagctcact tttaaagtag aggatatctt
      481 ttgtattaat tttacaaaga ttagttccaa tctgaactgt aataaagttt tctttccatt
      541 gttctaattt ttttagtgct tcttttaata ctttacatat tttttcagtt ttataaggtt
      601 ttaagatgta gttcaaagct cctacatcat atccatgaaa tacatagtca gaaaaacctg
      661 tgacaaagat gattatagta tcttttgaat gttttcgtat agtttttgct atgtcaacgc
      721 cattactttt tccaagttca atatctaaaa aaataatatc tatgtaataa ttttcgtgtt
      781 ctagattgta tactagactt tctccatttg aatattcttt tatttggtag gggtagcccc
      841 acaaatccat ttctcttgta atgattttat tcagcatgtt tctttgttgt ttttcatcat
      901 cacaaataca aatcgaagtc aattttttat ctcctttcaa gttttatatt tatatagtta
      961 agtatataat atattttgct acctagatat ataaaatgaa ataacaatta tatccagata
     1021 gcaaaataaa attttttata ttagtatact tacacttaaa actattctat taaacttatt
     1081 ctgaagtgct aatg
//
LOCUS       DAMJZE010000347         1091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMJZE010000347 DAMJZE010000000
VERSION     DAMJZE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.359"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..146
                     /locus_tag="PC541_003877"
     CDS             <1..146
                     /locus_tag="PC541_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841256.1"
                     /translation="DTDNLFESDNVEGALKELATKQNLLDTEVNGQREKGINIANNLI
                     DMI"
     gene            160..843
                     /locus_tag="PC541_003878"
     CDS             160..843
                     /locus_tag="PC541_003878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841257.1"
                     /translation="MTTKLTDNASLGELMTALQSVQTDFQTGKNNLSSALGNPFVGTD
                     KLETTKTKIETLKNSLAVNLTNKNVSANETESIQSLINKVGNIKISNYAAGTTSVVKN
                     TSLYAKTFYLNSTSNPSYWISITNLNFLPNVFIAECEYTTSDKWNKYYVIATSYFPSS
                     TVILTDFVLNVSFNCEFSASTLNSGASKLYENNKRDVYITGGSIRVPAFTESSSTLSS
                     YKWYALKIQ"
     gene            864..>1091
                     /locus_tag="PC541_003879"
     CDS             864..>1091
                     /locus_tag="PC541_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841258.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDIITQLHCIDVPYGS
                     IDYEKYRIIGINIETKEPISEEIPVYVNEEEK"
BASE COUNT          462 a          131 c          177 g          321 t
ORIGIN      
        1 aagatacaga taacttattt gaaagtgaca atgtagaagg agcattaaaa gaattagcaa
       61 caaaacaaaa cttgttagat acagaagtaa atggacaaag ggaaaaagga attaatatag
      121 caaataatct aatagatatg atataagtga ggtgaaaata tgacaacaaa attaactgat
      181 aatgctagtt taggggagct tatgacagca ttgcaaagtg tacaaactga ttttcaaact
      241 ggtaaaaata acctatctag tgcattaggt aatccttttg ttggtacaga taaattagag
      301 acaactaaaa caaaaataga aacattaaag aattctttag cagtaaattt aacaaataaa
      361 aatgtttctg caaatgaaac tgagagcata caaagtctaa taaataaagt tggaaatata
      421 aaaatatcta attatgctgc tggaacaaca tctgttgtaa aaaatactag tttatatgcc
      481 aaaacattct acttaaacag cacaagtaat cctagctatt ggattagtat cactaatcta
      541 aactttcttc ctaatgtttt tattgctgag tgtgaataca caacaagtga taaatggaat
      601 aaatattatg ttattgccac ttcttatttt ccttcatcca cagttatact aactgatttt
      661 gtattaaatg tatcatttaa ttgtgagttt agtgctagta ctctaaacag tggtgcttca
      721 aagttatatg aaaataataa aagagatgtt tacattactg gaggaagtat tagagtacca
      781 gcctttacag agtctagttc tactctatct tcttataaat ggtatgctct taaaatacaa
      841 taaattaaaa atgaggtgat aaaatgaata gagcaaatag aataatttat gaccaaacag
      901 gtaaaatact cttgcaaaca ggagaagcaa caggggatat attggagcat gatataataa
      961 cacagttgca ttgtattgat gttccatatg gaagtataga ctatgaaaaa tatagaataa
     1021 taggtataaa tatagaaaca aaagaaccaa tttcagaaga aataccagta tatgtaaatg
     1081 aagaagaaaa a
//
LOCUS       DAMJZE010000348         1077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMJZE010000348 DAMJZE010000000
VERSION     DAMJZE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.360"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(205..936)
                     /locus_tag="PC541_003880"
     CDS             complement(205..936)
                     /locus_tag="PC541_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2841259.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT          410 a          153 c           89 g          425 t
ORIGIN      
        1 ttatataaat taaaatatgt atttataatg attaaaaaaa ttgaaattat cgaatataaa
       61 ataaaacacc aaaattttga attaaaattt gtaaaattga ttagaaaata gtattgcact
      121 attaccatta aaattctata tacaataaaa gagaagaaaa aactgtactt taaaaataaa
      181 tcggaagaaa ataaattaat catcttatat cctcctattt ttcatgaact caagcataac
      241 ttttgaaaat atttgttgac gtagtctaga aacaggtatt ctatctccac tttccataac
      301 tgcattacca ttttcatatc catatacata ttgtaaattg attatataac tacgatgaca
      361 cctaaaaaaa tttgttggta aacttctctc taaattttca atagtctcat aataatctac
      421 agtctctttc tttaaatgaa cataaatctt tctttttata acttcacaat ataaaatatc
      481 agcaagtttt aatttccaat atgaataacc tttttgtatt gtgatataat tactttcatt
      541 atcatcaagt tttgtaacta ttttatccaa agttttatat agtttctcat tagatagagg
      601 ttttatcaga taatttatag catctacttc aaaagcatca aatacaaatt catttaaaac
      661 tgtaacgaaa attataaagc actcattacc atatgacctt aattttctag ctgcctgcat
      721 tccgttaatc tcttccatct gtatatctaa aaatacaata ttaaactttt catgattgca
      781 tagtaaatct tctactttat taaaaagctc tatattatat aataaattct tctgcttcat
      841 gtataattct atattttcct ttaaatgatt tagcatatag gattcatcat cacaaatggc
      901 aattgaaatc aatatataca ccttctttct tatcattatt ctaattatta tataaagtta
      961 attttttctg ttcaattaat atgatctaat atgttgaata tttgttccaa aatgtaataa
     1021 tctcgctttt catagtatat ctaatttgaa tttgtgtatc aatattataa taaacta
//
LOCUS       DAMJZE010000349         1076 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMJZE010000349 DAMJZE010000000
VERSION     DAMJZE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.361"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(221..712)
                     /locus_tag="PC541_003881"
     CDS             complement(221..712)
                     /locus_tag="PC541_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2841260.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          413 a          158 c           92 g          413 t
ORIGIN      
        1 aaataaaaat atctacaact atatattgca aaaatttaaa actaatttat atatgctaat
       61 ataaatattt ataaagacat aagacaaatt tacctacaga tttaaattta acttatatat
      121 tttaaaaggc taatataata tgtattttct tctatacaat caaaaggctt catatctaag
      181 atatgtgatg acactaatat tcacaatttt ttagtaatta tcacattata aaaaatatta
      241 cagctagtcc atcatcagat gcttcttgta taaacttttt aagctcaatt agatttgccc
      301 atacatattc aaaaaaatca tcttcactct catcattcat aacaggataa atattatttt
      361 ctaacatctc attaatatta aatctttttc taaatgcttg ttcttccaaa ctgttcattg
      421 cttttgatag tttttttaca tcatttgctg taatcaacat gacaggaaat tctccatcaa
      481 tttccataag tatgtttcca cttagtacta atttgctaaa tatattatca tcatctccac
      541 caaaaggaca acctgtcaaa gtaaagtgaa tggcatgcca tgctttatca atattgatag
      601 tactattttc gttggcatct tcaaaaacaa aatcttgtaa gttttcttct gcttgctgta
      661 atttaaaaac attctcttca cttattttag tataacatcc tatcatcccc ataattacat
      721 tcctcctaag tagcgttttt ttaattgtaa cttataatta aacttagtta caataaaata
      781 tattgagaaa agtattattt catcttaaat ttaaatacct atattatcaa ctaaaattct
      841 gtgtttatag cgtaagtatt attatttttt cctaaaaatt aaatgaatca gaagttctat
      901 aggcaatatt gactataaat aagaactatt acttttaaaa aaaaatttaa gaaattatac
      961 tgtagaatca tgctctactt attagcttta aataaaaaat aaatcatgtt ctcaaattaa
     1021 ataataaata acaataaatt taaattttaa actataagag tttctgaagt gttaat
//
LOCUS       DAMJZE010000350         1063 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMJZE010000350 DAMJZE010000000
VERSION     DAMJZE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.362"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(214..897)
                     /locus_tag="PC541_003882"
     CDS             complement(214..897)
                     /locus_tag="PC541_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2841261.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          413 a          165 c           81 g          404 t
ORIGIN      
        1 tactatactt gaaatacttc tattaatcta tatattgcct actatactta agacatttta
       61 attaatttat acactgctta ctatacttaa aatacttcta ttaacttata cattgcttac
      121 tatattttaa attctttaat taatctatac aatatttgct atatataaag tatatcaaat
      181 aacagcttca tttttttgta aatttacaga aagttaattt tcattaaatg aatcatcttt
      241 attgataaac ataattccaa agaccactgc caaaataaaa aaacatatta gtattgattt
      301 ttccacgaaa atttttgtaa aaaaagttcc taaaatagct ccagatataa agaatataat
      361 aatagcataa tactgtaaac tcttttttat cataaaaata ttcttagtat gtatataatg
      421 aaataaagtt tctgtagcac ttcttaaatt tccagtacac attgtcgttg caaaactatt
      481 cccatttact tttctaaaac tctctacttg cattgaacaa ataaaagaaa tagcaacatt
      541 tacaaacata ttaaatcttc ctctaggaat aaatgaaaca ataagtattg taatcatttc
      601 aattataatg ataacctgac gccaatgaat ctttgtctta ttatttgtaa aatactttct
      661 tatcttctcg gctataaata cacctaacat aaatgccaaa ataggaatca aataataaaa
      721 tgattccttt acattacctt ctgctatatt aagcccaaat aaaactatat ttccagtttg
      781 tgcattagca aagactttac ctcttaccac ataagtataa atatccaaaa atcctcctgt
      841 tattgccaac aaagaacata aaaagaatga ttcagacatc tgactcttgc ttgtcatata
      901 taaacctctc gctttctaat gatagataca aaatcaaaat cattttagat tctatttttc
      961 attgatataa aaaaattatt tatatttaaa tattgtctat atttatacat tatttgtatt
     1021 tatatagcat tcatatttat ttatatttaa acattatcta tat
//
LOCUS       DAMJZE010000351         1057 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMJZE010000351 DAMJZE010000000
VERSION     DAMJZE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.363"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            41..997
                     /locus_tag="PC541_003883"
     CDS             41..997
                     /locus_tag="PC541_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2841262.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          431 a           88 c          161 g          377 t
ORIGIN      
        1 aaaattttta gaatgtaaat tgaaatttag gagtagatgt atgtatatac tgaatttaaa
       61 tacaagagag actattgaag attttagaga taaattttat gtagcagaaa attcttatct
      121 tatattaagt gctccaaaaa atttaaaatt gcttaaggaa actttggata tagatgaaat
      181 aacttttaat gattgtctta aatttgatga aataaccaaa cttgatttat ttgataatta
      241 cgatttttta agcctaaata catttgaact aagagatgga gaagctgtta tagaagaggt
      301 aaatatgtat ttatcagata attttatatt ggttgtagtg aatgaagaac actttttatt
      361 tgaatttgta aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc cagttattaa
      421 tctatttaag ataaactact tgattcttag agaagttata aaaaatggtt ttgagtcttt
      481 ggaaaaagtt gaagagttaa ttcttcaaat agaagatgaa atgatggaca acattaataa
      541 gaatcatgta tctagaataa gtgatgtaag aggattaaca agaattatag ttaagaatac
      601 tagacctctt ttgtatattg gggatagaat agttaaggaa aatataagat atttaaaata
      661 ttcaaatgta aaaaaatata atttagaaaa ttttcaaggt atagattttg gaattgacaa
      721 attatacaat ttcgcattgt caacacgaga gttagcagat aaattattag atatttattc
      781 ttccagagta ggtgagaaaa caaataactt aattacaaaa ttaaccttgt taacagccat
      841 atcagcacca cttacaatta taactggtat atatggaatg aactttagat atatgccaga
      901 attaaactgg atttatggat atccagcaac tttatttttt atgctatgta taatttttgt
      961 tggaattata atatttaaaa taaaaaaatt attgtaaatt acagatgatg tttaattgat
     1021 tttataaaaa tacatcaatt gagaagctga tttaagt
//
LOCUS       DAMJZE010000352         1054 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.364, whole genome shotgun sequence.
ACCESSION   DAMJZE010000352 DAMJZE010000000
VERSION     DAMJZE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.364"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            241..921
                     /locus_tag="PC541_003884"
     CDS             241..921
                     /locus_tag="PC541_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2841263.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          466 a           64 c          168 g          356 t
ORIGIN      
        1 taaaaaatca aatgaaatac caaataaagt gtatatctta aaataactta ctagagatac
       61 aaggatgcta tgtgaatttt gaagtgtgca gtttagataa taaatatata taaataataa
      121 agaaaatgga tttatagtat ttaaaatttc ttataagggt aaaaaatgct attagaaaat
      181 gtactaaaaa tatttttcca attctttata gaaaaataat gatatttagg agagattgta
      241 atgcaaattg atgtattgat aattgatgga gataagaata gttctcaaga gctgaaaaat
      301 tttttagaag gaaaggggat aaatgtctat ttagcatata attgtaaaga tgctattata
      361 aaaatattta gcaagaaatt tgacttaata tttttagaga taattttagt tgatggtgat
      421 ggttgggatt tttgtaaaaa aattagaaat ataactactt gcccaattat tcatataact
      481 tatataaatg aaaatcaaag tattttaaat gctttaaatt ctggaggaga tgattattta
      541 ataaagcctt taaatttaga aatattatat gcgaaagtta aagctatttt aagaagaaga
      601 gatttatttt taaataataa taaaaataat ataaaactag aagattataa tagagctagt
      661 ggagtaataa agttagaaaa taaaatgata aagttaactc caacagaaaa tagattatta
      721 aattacttta tagagaatgc tgaaaagact ctaactatta aggagatata tgaacatgta
      781 tggatgaatg agtatttaga tgataattat agtgtagtgg ttgctgtgag tggattaaga
      841 aagaaaatag agaaagatta taaaaatcct caaaaaatta taacgataag agaagtaggg
      901 tattacttta acaagatata aagctttata aacagaatga atgataaggc atagataata
      961 tgttagtata ttataagagt ttttgttcta acgatatatt tgtaaaatta gatttaattt
     1021 acttaattaa attatagcag tagaataaat ataa
//
LOCUS       DAMJZE010000353         1045 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.365, whole genome shotgun sequence.
ACCESSION   DAMJZE010000353 DAMJZE010000000
VERSION     DAMJZE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.365"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            7..81
                     /locus_tag="PC541_003885"
     tRNA            7..81
                     /locus_tag="PC541_003885"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            87..172
                     /locus_tag="PC541_003886"
     tRNA            87..172
                     /locus_tag="PC541_003886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Leu,seq:taa)
     gene            188..263
                     /locus_tag="PC541_003887"
     tRNA            188..263
                     /locus_tag="PC541_003887"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Met,seq:cat)
     gene            271..345
                     /locus_tag="PC541_003888"
     tRNA            271..345
                     /locus_tag="PC541_003888"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Glu,seq:ttc)
     gene            360..433
                     /locus_tag="PC541_003889"
     tRNA            360..433
                     /locus_tag="PC541_003889"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:392..394,aa:Gly,seq:tcc)
     gene            439..514
                     /locus_tag="PC541_003890"
     tRNA            439..514
                     /locus_tag="PC541_003890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:472..474,aa:Val,seq:tac)
     gene            520..596
                     /locus_tag="PC541_003891"
     tRNA            520..596
                     /locus_tag="PC541_003891"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:554..556,aa:Asp,seq:gtc)
     gene            607..681
                     /locus_tag="PC541_003892"
     tRNA            607..681
                     /locus_tag="PC541_003892"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:639..641,aa:Gly,seq:gcc)
     gene            691..764
                     /locus_tag="PC541_003893"
     tRNA            691..764
                     /locus_tag="PC541_003893"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:725..727,aa:Arg,seq:tct)
BASE COUNT          289 a          228 c          248 g          280 t
ORIGIN      
        1 aaatgttcca aggtagctca acggtggagc aaccggctgt taaccggtag gctgtgggtt
       61 cgagccccac ccttggagcc aaaaaagccg aagtggcgga actggcagac gcacaggact
      121 taaaatcctg cgggacttac ctctcgtacc ggttcgattc cggtcttcgg cacttataaa
      181 acaatatcgc ggggtggagc agttggcagc tcgtcgggct cataacccga aggtcgtagg
      241 ttcaagtcct gcctccgcaa ccattaaaac ggctcgttgg tcaagaggtt aagacaccgc
      301 cctttcacgg cggtaacagg ggttcaattc ccctacgagt caccaattca attaaatatg
      361 cgggtgtagc tcaatggtag agttctggcc ttccaagcca gctgtgaggg ttcgatcccc
      421 ttcacccgct ccaaaaatgg gaccatagct cagctgggag agcacctgcc ttacaagcag
      481 ggggtcacag gttcgagccc tgttggtccc accattcgcg gcctggtagt tcagttggtt
      541 agaatgccag cctgtcacgc tggaggtcga gggttcgagc cccttccagg tcgccaaatt
      601 aaataagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa gtcgcgagtt
      661 cgaatctcgt ttcccgctcc ataataactt gggtgcatag ctcagctgga tagagcaacg
      721 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcattaaga aaagtttact
      781 ataaattata gtaaactttt atattgttta aaaagacaca ggaaacatgt atattacctg
      841 tgtcttttta tttataaact ttaaactatg taaaaattac agtatattct aatagagtta
      901 cagtatattc attaatattg taaacatacg gagggtaatg tataatagtc tacaagatgt
      961 ttatgaaggc atcagttaaa tataataaat ataactaatt agtaagagtt atatacatta
     1021 tattgtatac tttatggtat ttata
//
LOCUS       DAMJZE010000354         1003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMJZE010000354 DAMJZE010000000
VERSION     DAMJZE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.366"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            69..821
                     /gene="cwlD"
                     /locus_tag="PC541_003894"
     CDS             69..821
                     /gene="cwlD"
                     /locus_tag="PC541_003894"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2841264.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          444 a          105 c          153 g          301 t
ORIGIN      
        1 ataatatatt aataccaata aataaagata tatcaatatt aaaaaaattt aaatgtttgt
       61 actaatatat ggaatattat agtgttttta taagtataca tatacagggt gatgttgtga
      121 gaaagtacat aaaacatata atttttagtt ttgcaatgat atgtctagta gtagtatcca
      181 tatttgaaat taaaaatatt tctgaagatg ttatcaagta tatgccagta acaaacaaaa
      241 ctataatttt agatgctggt catggaggca ttgatccagg tgcattaaat aaggataaga
      301 gtacatctga aaaggatatt aacctagcaa taacacttaa gcttagagag cttatagaat
      361 caagtggtgg ccttgtaata ttaactcgag aagatgacag tagcctttat aaagaagaaa
      421 ataataaaac tacaagacaa aaatataatg agaatttgaa aaatagaaaa gaaataatat
      481 ctaattctaa tgccaatatg tttgtctcta tacatcttaa tgcatttgaa caatcaaaat
      541 actatggagc tcaaactttt tatcctaaag acaaacaaga cagtaaagaa ttatcgaagt
      601 gtattcaaga agaacttaaa agagtagtag ataagacaaa caacagagaa gtcaaaccaa
      661 gggatgatat atatcttttg aaagaaaata atattccatc agtactgata gaatgtggtt
      721 ttttatcaaa tgaaaaagag tgtaaactct taactgatga aacatatcaa gaaaaaatag
      781 catgggcaat ctacatagga atacaaaaat atttaagttg aaataactat aaaaataggt
      841 ctaaatatgt aaatagcttg tattaaagca gttaaaatgc attaatatag gctattttta
      901 ttttgacaaa aaaatattta aaataaaagt taaaaagttg ttgacttaga ataatataga
      961 tgatattata tatgagtgcc caaaaggagc accaaaataa gac
//
LOCUS       DAMJZE010000355          993 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.367, whole genome shotgun sequence.
ACCESSION   DAMJZE010000355 DAMJZE010000000
VERSION     DAMJZE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.367"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            196..>993
                     /locus_tag="PC541_003895"
     CDS             196..>993
                     /locus_tag="PC541_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2841265.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVV"
BASE COUNT          357 a          108 c          178 g          350 t
ORIGIN      
        1 actaaattga aataatcact ttttcaagaa tgttataatt tttttattgt attctttttg
       61 ctatagtcta tgaataaatc aatagaaaat cttctaagtt gcttttaaat aatgacttta
      121 tggttcaatt gtactaaaat agtatatgta ggttacaaat aatctaataa atattaaagt
      181 ttggaggtaa tagatatggc agattgtaat tcatgtccat caaagggaaa ttgtaatagt
      241 caatcaaatt gttctattga aaataaccct aacaataaat ttggaaaaat aattggtgtt
      301 atgagtggga agggtggagt tggaaaatca actgtaactg ctttacttgc taataagcta
      361 aacaaaatgg gatataaggt tggtatatta gactcagata taactggacc aagtattcct
      421 agacttatgg gtgtaaaaaa tgtaaaagct tattctgatg gctcttatat ttaccctgta
      481 gaaaactcaa ataacataaa agtaatgtct ataaacctta tgatagatga tgaaaatgaa
      541 cctgttgttt ggagaggacc tttacttgga ggggttgtaa agcaatttta tactgatgtt
      601 ctttgggaag aattagacta tttattaata gatatgcctc ctggtactgg agatgttgcc
      661 ttaacagtta tgcaatctat tcctataagt ggaatagtta tggtttcagt acctcaagat
      721 ttggtatcta tgattgtatc aaaagctgtt aatatggcta aaaaaatgaa cataaatgtc
      781 cttggagtta ttgaaaatat gagttatatc caatgtcctg attgttctaa aaaaatcaag
      841 ttatttgaag gagaatctac tgaaaagttt ttagatgatt tagacttaga gcttttagga
      901 gaacttccta tgactaaaga aattatagat attactcata atggagttac agaaattagt
      961 gatgatttag attcaatatt gactaatgtt gtt
//
LOCUS       DAMJZE010000356          961 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMJZE010000356 DAMJZE010000000
VERSION     DAMJZE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.368"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..896
                     /locus_tag="PC541_003896"
     rRNA            <1..896
                     /locus_tag="PC541_003896"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          243 a          208 c          283 g          227 t
ORIGIN      
        1 agagactcgg tgaaatcata gtacctgtga agatgcaggt tacccgcgac aggacggaaa
       61 gaccccgtgg agctttactg tagcctgata ttgaaattcg gcacagcttg tacaggatag
      121 gtaggagcct ttgaaacgtg agcgctagct tacgtggagg cgttggtggg atactaccct
      181 agctgtgttg gctttctaac ccgcaccact tatcgtggtg ggagacagtg tcaggcgggc
      241 agtttgactg gggcggtcgc ctcctaaaag gtaacggagg cgctcaaagg ttccctcaga
      301 atggttggaa atcattcata gagtgtaaag gcataaggga gcttgactgc gagacctaca
      361 agtcgagcag ggtcgaaaga cggacttagt gatccggtgg ttccgcatgg aagggccatc
      421 gctcaacgga taaaagctac cccggggata acaggcttat ctcccccaag agttcacatc
      481 gacggggagg tttggcacct cgatgtcggc tcatcgcatc ctggggctgt agtcggtccc
      541 aagggttggg ctgttcgccc attaaagcgg tacgcgagct gggttcagaa cgtcgtgaga
      601 cagttcggtc cctatccgtc gtgggcgtag gaaatttgag aggagctgtc cttagtacga
      661 gaggaccggg atggacatac ctctggtgta ccagttgtcg tgccaacggc atagctgggt
      721 agctatgtat ggacgggata agtgctgaaa gcatctaagc atgaagcccc cctcaagatg
      781 agatttccca acttcggtta taagatccct cgaagatgac gaggttaata ggttcgaggt
      841 ggaagcgtgg tgacacgtgg agctgacgaa tactaatcga tcgaagactt aatcaatttt
      901 taaaaaacgt tttgcgacgc aaaatcttac ttactatcta gttttgaatg tataatcatt
      961 c
//
LOCUS       DAMJZE010000357          951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMJZE010000357 DAMJZE010000000
VERSION     DAMJZE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.369"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            289..756
                     /locus_tag="PC541_003897"
     CDS             289..756
                     /locus_tag="PC541_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDF2841266.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          368 a           64 c          159 g          360 t
ORIGIN      
        1 attaatattc ccttgaaatt aaaaaaacat agatatttca gtaatctaaa atagtctaca
       61 aaaataacag ttttagcaaa attaataaga atacatatag atttaaacct aaaagtatga
      121 acatgtctgt tttttacaag attaaaattt atattatatg ataaaattaa cttaactatt
      181 tcaataagac ttataatttt tttctttata ctagacctta ttgaaacctt tatagtttaa
      241 ttctaattat atattataaa taaagtatgt gtatattaag gaggtaatat gaagttttta
      301 aatgttttag tagtagttga agatattgag aaatcaaaaa agttttatta tgatgttttg
      361 gggttaaagg taatctgtga ttttggagaa aatgttgtat tagaaggtaa tatatcatta
      421 caagaaaaaa agctttggtt agaatttatt aataagagtg acagtgaagt taagttcaat
      481 ggaaatgatg ctgagttata ttttgaggaa gataattttg atacttttgt tgaaagattg
      541 agtacaatga aagatataga ttatgttcat ctagctatag agcataggtg gggtcaaaga
      601 gcaattagat tttatgattt agatgggcat attattgagg ttggagaaac tatgtcatct
      661 gtgtgtagac gttttttaga tagtggtctt tcaatagatg aggtagctaa aagaatggat
      721 gtaactgtcg aatatattga atcagtttta gaatagatgg attatattaa agtaaaattg
      781 aggtgaaggc tgtaagtaaa cttgtgtgaa aacaaatttg cttacagttt ttatttttct
      841 aaaataaaga atataatact tgtttagctt tatttgagtt tttaaaaata ttatgatatt
      901 tcttatatat ttatactaaa gtttaaattt agaaaaaata tataaaagat a
//
LOCUS       DAMJZE010000358          904 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMJZE010000358 DAMJZE010000000
VERSION     DAMJZE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.370"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            103..357
                     /locus_tag="PC541_003898"
     CDS             103..357
                     /locus_tag="PC541_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841267.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            402..725
                     /locus_tag="PC541_003899"
     CDS             402..725
                     /locus_tag="PC541_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841268.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          369 a           73 c          150 g          312 t
ORIGIN      
        1 aattttggaa atagatgtaa atatatgttg tattgtataa taagagtaat ataaaatcta
       61 gtaaaaataa tcaaataaat ttagcttatg agagggataa ctatggaaaa taaggatttt
      121 aaattaacaa tatgtatatt tgcttttgta attggagtag tagtattatt ctgttgtata
      181 ggtttaggtg aatttagtgc agagcatatt attcaacaaa atggtggaat catggatact
      241 gaccaatata atatttatct agaccaatct atttctcaat atagaaattt tggttctata
      301 ttagctctgt taggaggaat agggatattg tttgataaat atatcagaaa atactagaga
      361 ttatgttttg tctaaaataa attcttatga ggagataaaa aatggcaaca gaaatattta
      421 ctgtaaaaat tacaccctta gaggcacaaa aaatgataaa agaaaatctt gatgcagatt
      481 tagtttttag tgattcttat aatttaggag atgataaatt tatactaatt acaacttttg
      541 agaagtatta tgcaagaaat aatagtgatg caggattaat agtagtttat gaaaatacca
      601 caggaaaaac agttattaaa gctacttcaa caggcagtgc aattggaatt ttaaaaattg
      661 attggggtgc aggtggaaat ttacttaaaa gagtaaaaaa catacttgct gattatatta
      721 tttaattatt ttatctaact gataatatgt caagaaaatc tcgtaaaaga ggttaagaac
      781 atttttataa tatatagata aataagaatt tgaaggtaaa gtttttaagg tataaagaaa
      841 aataatataa taaaacgaat tggctttata attctttata aggcaaagag cacatttctt
      901 tata
//
LOCUS       DAMJZE010000359          895 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMJZE010000359 DAMJZE010000000
VERSION     DAMJZE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.371"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            53..751
                     /gene="tcdC"
                     /locus_tag="PC541_003900"
     CDS             53..751
                     /gene="tcdC"
                     /locus_tag="PC541_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2841269.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT          358 a           95 c          164 g          278 t
ORIGIN      
        1 taagttttta acttttaaaa taaaatatat ctaataaaag ggagattgta ttatgttttc
       61 taaaaaaaat gagggtaacg aatttagtaa tgaaggaaaa ggaagctcta agaaaataat
      121 taaattcttt aagagcacaa agggtattgc tctactggca tttattttag gcgtgttttt
      181 tggcaatata tcctcaccag cttgttctga agaccatgag gaggtcattt ctaaccaaac
      241 atcagttata gattctcaaa aaacagaaat agaaacttta aatagcaaat tgtctgatgc
      301 tgaaccatgg ttcaaaatga aagacgacga aaagaaagct attgaagctg aaaatcaacg
      361 taaagctgaa gaagctaaaa aagctgaaga agctaaaaag gctgaagaac aacgcaaaaa
      421 agaagaagag gagaagaaag gatatgatac tggtattact tatgaccaat tagctagaac
      481 acctgatgat tataagtaca aaaaggtaaa atttgaaggt aaggttattc aagttattga
      541 agatggtgat gaggtgcaaa taagattagc tgtgtctgga aattatgata aggttgtact
      601 atgtagttat aaaaaatcaa taactccttc aagagtatta gaggatgatt acataactat
      661 aagaggtata agtgctggaa ctataactta tgaatcaact atgggtggaa atataactat
      721 accagggata gctgtagaga aaattaatta atattgtttt gtattatagt taatatttta
      781 tattatagtc aatatgttta aagatgtttt tataattgca aataaacagt tacaaggctc
      841 taaattagtt tttgctttta gcatattatc tattttttat caactattaa ttatt
//
LOCUS       DAMJZE010000360          893 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMJZE010000360 DAMJZE010000000
VERSION     DAMJZE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.372"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            130..672
                     /locus_tag="PC541_003901"
     CDS             130..672
                     /locus_tag="PC541_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2841270.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          374 a           74 c          150 g          295 t
ORIGIN      
        1 aatatgttaa aaaaatgata atcacggaga acttttacct tcttcaatta atatattatt
       61 attagtaata actttaaaat tatattacta ttatataaaa attggcttaa aagaaggtga
      121 aaaaagtaaa tgaaaatatg cataacagta ggtcatagta tactaaaagg tggtaaaagt
      181 acaggtgtaa atggtatagt tgatgaatac aattataata aaaaattagc tcctatgtta
      241 gctgagatgc ttataagtca aggtaataca gtagatgtaa taatttgtcc agaaaggcac
      301 tttatgtcag aaagagaaga atttttctat agagttccaa aagtaaacag tggtaaatac
      361 gatttattag tagagctaca tttaaataaa gctgatggaa cacaatgtgg tacagaagtg
      421 ctttactatg gcactgaagg attagaatat gctaaaagag tatctaatag acttggagaa
      481 ttatttgaaa atagaggagc taaaaaaaga gaaaatttat atatattgag aaatacaaat
      541 ccagttgcaa tacagattga aagttttttc tgtgataatg tgaatgattg taacaaagca
      601 aatgaatcag gttatgatta tatagcacga ttgattacag aaggtatatt gaataaagat
      661 ataaatatgt gagattatgg tgatttaggt attgattaat accttttaat attagatata
      721 aataaaaata caatgtatta gtataattta aactttcaaa agatagatta gtttgattat
      781 tgacaagaga tacctccttg aaaatattga tttatcagta tatttaagga ggtatttaaa
      841 tataaaaatt taattaattt gctactaaac tataggttta tagagtatat act
//
LOCUS       DAMJZE010000361          873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMJZE010000361 DAMJZE010000000
VERSION     DAMJZE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.373"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            109..807
                     /locus_tag="PC541_003902"
     CDS             109..807
                     /locus_tag="PC541_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2841271.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          331 a          109 c          131 g          302 t
ORIGIN      
        1 ttttaatact aatataatta gtattatatt aaattctata ttacaacatt attttatagt
       61 ttgattaatt taacaatcac taatatatca aactgaaagg agatttttat ggaaaaaaaa
      121 tatttcgaaa ttcatggagt tccttcaatc ctttggggaa atccttcaag taaattatat
      181 atttacatac atggacaatg tggcaataaa gaggaggctg aagcttttgc taatattgca
      241 gttccacatg agtgtcaagt tttaagtgtt gatttgcctg aacatggaga acgtaaaaga
      301 gaaaaaaatt catttaaccc atggcatgtt gttccagaat tagtatctat tatggaatat
      361 gcaaagtgcc actggaatca aatttcgtta tatgcaaata gcattggagc atggtttagt
      421 atgttaagct ttgaaaaaga gaacttaaga gagtgtattt ttgtatctcc aattctagat
      481 atgcaacagc tcatttcaaa tatgatgctt tgggcaaatg tatcagaaga acagcttaaa
      541 catgaactaa ttatcccaac ttcatttggg catactcttt catgggaata tctacaatat
      601 gtaaaacaac atcctatcac tcaatggaat gtaccaacaa aaatattata tggtaaacat
      661 gatgaactta ctgaaatcgg tgttgtagag aaatttgtag aaaagtttaa ttgcaacctt
      721 actattatag aaaatggcaa acattggttt aacacaccac aagaattaaa ttcattatat
      781 gaatggttaa atactttagt taaataaaaa ataattatat tattaatatg attaaattat
      841 ttataaatgt tatttaatta attttatttt agc
//
LOCUS       DAMJZE010000362          822 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMJZE010000362 DAMJZE010000000
VERSION     DAMJZE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.375"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            37..270
                     /locus_tag="PC541_003903"
     CDS             37..270
                     /locus_tag="PC541_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841272.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            280..681
                     /locus_tag="PC541_003904"
     CDS             280..681
                     /locus_tag="PC541_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841273.1"
                     /translation="MENNMIDDIERRLQGFGYVLKDGDKWLIDFVREKIENIIKLDCN
                     ISEIPKELHNIEIDMIAGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNNILTFRCLRW"
     gene            675..>822
                     /locus_tag="PC541_003905"
     CDS             675..>822
                     /locus_tag="PC541_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841274.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLS"
BASE COUNT          354 a           74 c          161 g          233 t
ORIGIN      
        1 tgcaatacaa tttgttgttg aaggagctgg agcataatgg cacaagtaag aaaattaaat
       61 agaatattaa ctatagaaga atgtaaaata gatgatttct tagagatggg atatgatttg
      121 atagatgaaa ctggtaaggc agtaaagtat ggcaagtcat taaatgtaaa agatttaata
      181 gctgaaaata atattttaag gtcaaaagtc gagtctttag aagaagaaaa taagcagctt
      241 aaagagaaaa ataagcttac taaaaagtag gtgaaaattg tggaaaataa tatgattgat
      301 gatatagaaa gaagacttca aggatttgga tatgttctaa aggatggaga taaatggtta
      361 atagattttg taagagaaaa aatagaaaat attattaaac tagattgtaa tataagtgaa
      421 attccaaaag agttacacaa tatagaaata gatatgatag ctggagagtt cttatttacc
      481 aagaaaaata tggggcaatt agatatagaa agtattaact ttgaagctgt agaaaagtct
      541 atatcagaag gtgatacaaa ggtagatttt gctataggaa gtggctctca aacaccagaa
      601 caacgctttg atagcttagt agcttatctt actacttatg gtaagaataa tatattaacc
      661 tttaggtgct taagatggta agtaaaacta gaaaagcaat agaaatgtta tatagagata
      721 aatgtactat agttgagtat cagccaatta aagaccctgt aacaaaacga actaacaata
      781 aagaagtgat tgtattagaa aatcaaccat gtaaactttc at
//
LOCUS       DAMJZE010000363          810 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMJZE010000363 DAMJZE010000000
VERSION     DAMJZE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.377"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>810
                     /locus_tag="PC541_003906"
     CDS             <1..>810
                     /locus_tag="PC541_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2841275.1"
                     /translation="TLNPLEIKSKITGNSIIFRGVNDAKQREKLKSINFSKGKLTWVW
                     CEEATELMESDIDILDDRLRGILTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIF
                     THHSTYLQNRFIDEAYYRRMQMRKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTE
                     FEYFDNMRLSQDFGFNHANVVLRIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEK
                     NLFMYCDSAEPDRIKMWKSAGYKAKGVKKGPGSVKAQIDYLKQLRIHVHPSC"
BASE COUNT          325 a           92 c          155 g          238 t
ORIGIN      
        1 actttaaatc ctttagaaat caaaagtaaa attactggta attctataat tttcagaggg
       61 gttaatgatg ctaagcaaag agaaaaatta aaatcaatta acttctcaaa aggaaaatta
      121 acatgggttt ggtgtgaaga agctacagaa cttatggaaa gtgatataga catactagac
      181 gaccgtttaa gaggtatttt aactaatcct aacttgtatt atcaaatgac gtttacattt
      241 aatccagtct cagctaccca ttggataaaa agaaagtatt ttgactataa aaatgatgat
      301 atatttactc atcatagtac ttatctacaa aatagattta tagatgaagc ttactataga
      361 agaatgcaaa tgagaaaaga gcaagaccca gaaggatata aagtctatgg acttggagaa
      421 tggggagaaa ctggtggagc aatacttaaa aactatgtta tacatgagtt tcctacagaa
      481 tttgagtatt ttgataatat gaggttatca caagactttg gatttaacca tgcaaatgta
      541 gtacttagaa ttggctttaa ggatggagag ttatatatat gtaatgaaat atatgtacat
      601 gaaatggata cctcagaaat cataaagatt gcaaatagta aaggtttaga aaagaatcta
      661 tttatgtact gtgatagtgc tgaaccagat agaattaaga tgtggaagag tgcaggatat
      721 aaagctaaag gagttaaaaa aggaccaggg agtgttaaag ctcaaataga ttatttgaaa
      781 caattaagaa tacatgtaca tcctagttgc
//
LOCUS       DAMJZE010000364          787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMJZE010000364 DAMJZE010000000
VERSION     DAMJZE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.378"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..>787)
                     /locus_tag="PC541_003907"
     rRNA            complement(<1..>787)
                     /locus_tag="PC541_003907"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAMJZE010000365          785 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMJZE010000365 DAMJZE010000000
VERSION     DAMJZE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.379"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     regulatory      <1..81
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            164..649
                     /locus_tag="PC541_003908"
     CDS             164..649
                     /locus_tag="PC541_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2841276.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          349 a           66 c          123 g          247 t
ORIGIN      
        1 aatattagat aaatctaatg acgcaaaact atagggacta aaatctttat gtaataaagg
       61 tcatgtcagc cagttgccaa aaagataata attctttttg ttaaaagtat ttttagagtt
      121 atatatttta ataacttaat atttggcaag aggagggctt aagatgatat ctgaattaat
      181 gttaaaaaat tttcgcaaat ctagcatcaa ttttaagact aaaattaaat tgaatttaag
      241 agaaaaacca gacataaata gtttgaaatt aaaaagtata ccagaaggta aaattgttaa
      301 gttaaagtgt gtagatggaa tatgggcaga agttgagagt aattatgaca aaggttggtt
      361 actttataaa tatttagaaa gattaagtaa tgctaaaaac aatagtaatg atacaataaa
      421 aaatagaaaa aagaattaca taggaaatat aagaacaaat ggattaagtt tagaacttag
      481 aaatgataga acattagaat ctaaagttat aactacaatc cctgatggtt ttaaagttga
      541 agtatgttat agcgttggaa aatgggcaag agttaatatc aataaaaatg aaaaacgata
      601 ttctggatat gtatataatc aatacataga agagcattta agtttataat acgatactag
      661 caagctttaa atatattaat aaatcgcaaa aaagaatccc attaatggga ttcttttttg
      721 tagaaacatt ataacagaac taaagagaat aaattaacta atttattttt attataaagt
      781 aatta
//
LOCUS       DAMJZE010000366          781 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMJZE010000366 DAMJZE010000000
VERSION     DAMJZE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.380"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            183..689
                     /locus_tag="PC541_003909"
     CDS             183..689
                     /locus_tag="PC541_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2841277.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          313 a           78 c          134 g          256 t
ORIGIN      
        1 ttttatctat attttacact tataaaatat gaaaactact tattgcgaaa gtaattgatt
       61 tgttgtatta taatttacat gaattatgta ggacaatcaa aagtcagtat tataagagta
      121 catatattca aatttttaac aattaattta tataattaaa aaatgaaggg taggatgaag
      181 tcatgggttt tagagaagta aaaattgagg aattacagtt taatccattt actaagattg
      241 gcaaagaatg gttacttata acagctggtg attcagaaaa gtttaacaca atgactgcta
      301 gctggggtgg tgttggagtc tattggggta aaaatgttgt tacaacttac ataagaccac
      361 agagatatac aaaagaattt gtagatagta atgatacatt tacagttgcc ttctttgatg
      421 aaacgtatag agaggcttta aatatttgtg gtactatttc tggtagagat ataaataaaa
      481 tagaaaaagc aggacttaca ccatattttg tggatgatac agtagctttt gaagaagcta
      541 atatgattat agtatgtaaa aagctatatc atggtaatat gccaccagaa aactttgatg
      601 ctaaagaaaa tgataaaaaa tggtatccag agaaagacta ccatactatg tacatttcag
      661 aaattataaa agttcttgta aaagaataat tataataaaa aatatatgaa aagaacttat
      721 tataagtaaa tgactgtgct ttcttatctg atatctaagt agatgctagt aactaaataa
      781 a
//
LOCUS       DAMJZE010000367          776 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMJZE010000367 DAMJZE010000000
VERSION     DAMJZE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.381"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            46..612
                     /locus_tag="PC541_003910"
     CDS             46..612
                     /locus_tag="PC541_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2841278.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          297 a           68 c          146 g          265 t
ORIGIN      
        1 agtgatataa taataaaaaa atttttaaag agggtgatat tacatatgag aaaacttgaa
       61 gagatattag aatataataa atcatttgta gagaacaaag aatatgaaca atatgtgaca
      121 tcaaaacatc caaataaaaa aatagtagtc ttatcttgta tggatacaag attaactgag
      181 cttttgccta aggctatgaa tttgaaaaat ggagatgtga aactaataaa aaatgcagga
      241 gctactataa tgcatccttt tggaagtatt atgagaagtg ttttagttgc aatttacgaa
      301 tttgatgtag atgaggttat ggttgtaggg catcatggat gtggtatgtg taatgttgat
      361 acggataagt tacttggtaa aattttagat agaggaatat ctaatgatgt aatacttaca
      421 cttcgtagtg ctggaattga tacaaaacaa tggttacatg gattcgattc tgcagaagaa
      481 tctgtaaaag atagtattga tttaataaaa gggcatcctt taattccaga tggtattatt
      541 gtacatggtc taattatatc acctgaaact gggaagttag acgttgttgt aaatggatat
      601 gaagataagt agagtatcat aatgttatat aatcaagata ctgttgaata tgatgttgtt
      661 tcaaaattta ttttgggaca acttttttat ttgaaaatat atgaaaaggt ttattttaag
      721 ctttacaata aaaaatataa gtagctgact aattaaaaat cttactatta tttatt
//
LOCUS       DAMJZE010000368          753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMJZE010000368 DAMJZE010000000
VERSION     DAMJZE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.382"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>753
                     /locus_tag="PC541_003911"
     rRNA            <1..>753
                     /locus_tag="PC541_003911"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAMJZE010000369          740 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMJZE010000369 DAMJZE010000000
VERSION     DAMJZE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.383"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..254)
                     /locus_tag="PC541_003912"
     CDS             complement(<1..254)
                     /locus_tag="PC541_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2841279.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          302 a          110 c           87 g          241 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtata gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacacccctt tgaaaatatt ttaaatggtt tttaaaattt
      301 ggttttaaaa aatgattttg atataaaaaa accacacagt atatataact gtatggcaaa
      361 aattcgaata agccacacag tataatccat gtggctctat attatgatga taaaataaaa
      421 actttaacaa caattactat agccaacata gatacaatcc ttaaaaatca taatcctaag
      481 gggcttgtat ctatgttatg acaatttgtc ttaacttagg tacaaaccct aactaaattg
      541 gctactaaaa aatcgtactt gtttgtttaa taaagtcttc atatttacct ctccttttaa
      601 tttaataatt ttgaaaattt tgttttctgt tttgtatagt aattagttta atacagtaaa
      661 aaataaaagt caactataaa ttttgaaaaa ttatcaaatt atcagaaaat tgagtttaaa
      721 taaaaaaatt tttacatgta
//
LOCUS       DAMJZE010000370          698 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMJZE010000370 DAMJZE010000000
VERSION     DAMJZE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.385"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT          284 a          108 c           57 g          249 t
ORIGIN      
        1 aatgtaacta gataatcata agataacaca ttaaaagaaa attatgaaaa cttacattct
       61 gtatagttaa tataaaacat aagtaatata aatttatgta aagcaacaaa agatagattt
      121 acattccata tagttaatat aaaaccttag agaaagcgaa aacgtaccat taaaatcaaa
      181 tatttacatt ccatatagtt aatataaaac gttgtaatca tagttccaat tagccacttt
      241 aacatgcatt tacattccat atagttaata taaaacacca aaataaactt agtattttca
      301 acacttacac gtatacaact ctcctaaatt tgcagtgaac gagcaatagt gcaattgata
      361 acgcttatca tgcactctca acgccttata tttcaactgt tacgccaaat tttttcacaa
      421 atatcgctca ctgcaaaatt cctataattt tattatatca taaatatatt acttttgaat
      481 attgatacca atttatacca taataaaagc aaaaaaacgt aatctatcta cattagattt
      541 aaattctata acttaaagtt ttttacataa tatttatgct tgtttaatca atctaatata
      601 tcattcttgt atattgcttt taatagtctt ttttcttaaa taaatatcta tctgaacaag
      661 catagtttaa ttaaaaactt ttgtattccc ttaaaact
//
LOCUS       DAMJZE010000371          677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMJZE010000371 DAMJZE010000000
VERSION     DAMJZE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.386"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..>677)
                     /gene="dcm"
                     /locus_tag="PC541_003913"
     CDS             complement(<1..>677)
                     /gene="dcm"
                     /locus_tag="PC541_003913"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDF2841280.1"
                     /translation="FLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHKPKE
                     DEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLIREL
                     KEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQNRER
                     IFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGA
                     KTGLYFI"
BASE COUNT          193 a          149 c           89 g          246 t
ORIGIN      
        1 tataaagtat agacctgttt tagcacctcc acctcctgcc tgacttctaa tacatctagc
       61 aattccaact gcatcataaa ttctatttgt actatgagtt ggattattta gttgctcaag
      121 attttttcca ctttttcttt cgataggaaa tacttttcgt gtacttcgtc ctctaaaatg
      181 tccaacaatg aatattcgtt ctctattttg gggtactccg aagtttttag aattaagaac
      241 ttgccactct gcatcatagc cgatttcatc cagttcaacg agaactttga ggaaatcaaa
      301 tcctccatta acactaagta gatttttaac gttttcaata agtaaatact tgggtctatc
      361 ttcttctttg agttctctaa taagttttgt aactgtaaaa aataaacttg aacgttctcc
      421 tctgaatcca aattgtttcc ctgcaacaga aatgtcttga catgggaatc caaaacacca
      481 gacatctgct cttgggatat tttctgttct aatttctcta atatctcttt caaaccattc
      541 atcctccttc ggtttgtgca tggcattata acttaaattt gcgaatttat catattcgca
      601 atgtcccaaa catttatgtc ctgctttttc catccctagc ctaaagccac ctatccctgc
      661 gaataaatct aaaaatg
//
LOCUS       DAMJZE010000372          664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMJZE010000372 DAMJZE010000000
VERSION     DAMJZE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.387"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..>664)
                     /locus_tag="PC541_003914"
     rRNA            complement(<1..>664)
                     /locus_tag="PC541_003914"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAMJZE010000373          660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMJZE010000373 DAMJZE010000000
VERSION     DAMJZE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.388"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..634)
                     /locus_tag="PC541_003915"
     rRNA            complement(<1..634)
                     /locus_tag="PC541_003915"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          151 a          175 c          122 g          212 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccagttaa aaaggtttga tagattagaa
//
LOCUS       DAMJZE010000374          656 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMJZE010000374 DAMJZE010000000
VERSION     DAMJZE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.390"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            161..301
                     /locus_tag="PC541_003916"
     CDS             161..301
                     /locus_tag="PC541_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841281.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT          280 a           63 c           91 g          222 t
ORIGIN      
        1 attaattata aaaaataata ataaagacga taagtattaa aattttatgt ttccaatatt
       61 tatgatataa ttaatatggc aaggaataaa tatttgaaaa agctatgagt ggtgtttcca
      121 tggaagtttc ttcactttct tatgagagga ggtgaaaact atggaaaatc taatgatgag
      181 tattataact ggtgttatag ccagttacat ttacgataaa ttaaagagcc actctgagcg
      241 accaactaag agtggctggg aacttaatat aaagttccag aaaagtaaat cttcaaaata
      301 atttgttgaa ggaaacatca ctcaaagtta agtaaaatat gtttccttgc ttttattata
      361 tcacaaatta aaaatattaa cacaaaattt tgtgaatata tacttattat tgttctaaaa
      421 aaatatgtaa ataattgatt tgattgcatg gaattattga tgtaatattt aagttaaata
      481 tacacaatct caattaaaga agataagtac tataaattca tatcaaaaaa atacataaac
      541 ttttaagtta taattttata aaaagtcttt tagtatcatt atttcaagag gaattatcat
      601 atgaaaaaat aacttcatta gaaaagtgga taaaaaactg gtaagaagat gactaa
//
LOCUS       DAMJZE010000375          653 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMJZE010000375 DAMJZE010000000
VERSION     DAMJZE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.391"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            44..>653
                     /locus_tag="PC541_003917"
     CDS             44..>653
                     /locus_tag="PC541_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2841282.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISI"
BASE COUNT          230 a           57 c           84 g          282 t
ORIGIN      
        1 attttataaa aaatataatt taatcatttt tgaggatatt attatgttag atgcatttat
       61 aacaattgtt tcttctatta tagaaatatg gtcatgtaaa aaagtttttg attatactag
      121 tcagtcaaag accgacttaa aaaaactaaa tatgtatttt ctcttttctg tttttctcat
      181 tatttcttct ttatatataa atataggagc taatagtaga atcttgatat gtatatttgt
      241 aatgttcgtt ttctataagt ctaactataa ggttaatata tataaatgtc tggtaatctc
      301 atttttttat tggttaatat taataggtat aaatggatta agtatgttat tagtagcaac
      361 agtaagcaat ttggataata tgaatgtttt acttagtggc agtttatata gatatgaagc
      421 aatattcttg ggaaagataa tgttaggtat attactttat gtttataagg cttcttttaa
      481 aaattttaaa ataagtaaaa aagagatata catatgtatt cctatgatag caaatttatt
      541 gtcatttttt gtactttatg attatattat taatcttgta aaaatctctc ctgaaagaag
      601 aactgctttt ttagtaatag ttttactatt aatattatct aacatttcaa tct
//
LOCUS       DAMJZE010000376          604 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMJZE010000376 DAMJZE010000000
VERSION     DAMJZE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.393"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(103..582)
                     /locus_tag="PC541_003918"
     CDS             complement(103..582)
                     /locus_tag="PC541_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2841283.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          239 a          101 c           50 g          214 t
ORIGIN      
        1 agcattatag tttatgtatt ataccctaat atattttatt ttaaattact agttagtcac
       61 tcttttcggg ttatttatct cctatatatt aaagtgtcac atctaaatag ttaaatcatt
      121 atcctctttt atctttattg cctttacaaa tccatctgca attacaaaac tcataagaga
      181 agttataaaa tatacaaata ctggaacagt aaaataaaat ccaaatccta aatctagaaa
      241 tctcattcct tttccagtag taccaaaagt tgaaataaac tcaattagag agtttaaaag
      301 caaaagatat ccaataatcc taaatctttt tacattctct tttacaaata ggctatattc
      361 agaactacct aatacttcta tcaatgattt tataatgata aaatatgata cacctaaaat
      421 aaacattaat attccataaa tagtaccttg tagagttgct tctccactaa aacagaggta
      481 cataaaactt ttaaatcctc ctgcaacaat tactaatcct aaaagaaaaa ataaaatttg
      541 taatatacca cttagtaaat tttttataag tttattagac atcatgccaa ctcctttaaa
      601 atat
//
LOCUS       DAMJZE010000377          602 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMJZE010000377 DAMJZE010000000
VERSION     DAMJZE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.394"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..479
                     /locus_tag="PC541_003919"
     CDS             <1..479
                     /locus_tag="PC541_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2841284.1"
                     /translation="LSKLELLKTDCKPFQFIVSRQLGNKVLFNTNIKVSLEEYNIVED
                     AENGSDTKVAIKLKQYRDYSTKKLVPATPKTTSDAGRPNVKIEKKRVDSVNEPNTKTY
                     TVKAEDSLWAICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKYTIYKGQVLRLV"
BASE COUNT          244 a           75 c          122 g          161 t
ORIGIN      
        1 ttttaagtaa actagaacta ctaaagacag attgtaagcc atttcaattt attgtatctc
       61 gacaacttgg gaataaagta ctatttaaca ctaatattaa agtatcatta gaggaatata
      121 acatagtaga agatgcagag aatggctcag atacaaaagt tgcaataaag ttaaagcaat
      181 atagagatta ctcaactaaa aaattagttc ctgctacacc taaaacgact agtgatgctg
      241 gtagaccaaa cgtaaagata gagaaaaaac gtgttgattc agttaatgaa ccaaacacta
      301 aaacatatac agtaaaagca gaggattctc tttgggcaat ttgtcaaaag caattaggaa
      361 atggttcact atataagaaa gtatatgaac taaataaatc aatgatggat aaggcaaata
      421 aggggaagaa tttaagtaaa tatactattt acaaagggca ggtgttgagg cttgtctgat
      481 gatttagtgt tagcaaatga tagagatgta agattagtca tagcacattg ggaagatttc
      541 tatgagcctg tcgttttaga tggaatcaca tgggagatag aaagacgagg agcgccaagt
      601 aa
//
LOCUS       DAMJZE010000378          593 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMJZE010000378 DAMJZE010000000
VERSION     DAMJZE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.395"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT          235 a           43 c           73 g          242 t
ORIGIN      
        1 ataagaatgt ataaggtggt aaatttatat aaaaaagaat atgagttgta aaatattaaa
       61 taatattgta ctaattggaa taattttaac tttattatag attattttaa taccattagt
      121 actgatggtg tattttaaaa ttggtataag cattacaaat acgaatatgc caactgtaat
      181 atgtaatata agtagctata tacatatgtg ctgtcccata tattgtatca ttgtttatat
      241 taaaaagatt atgtaaattg gtatcaatca ataaatcatt ttcagaagaa atacctaaaa
      301 acttaaaaaa tacctatatg tgtatttagt tagatttgag atttttacat ttaattttat
      361 agtgacaata ttatattatt tttatgatgt ctatttatat gcaattacta taatatcaga
      421 acttatagta tcatttgttt cattatcaat aaggtttttt actttagttt tgtcaatttt
      481 attaaaaaaa gttattgata taaaagataa gaaggataaa actatatgac ggatttcaaa
      541 cataagagtg gatatttata taatagatat tttatagtta attttattag att
//
LOCUS       DAMJZE010000379          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMJZE010000379 DAMJZE010000000
VERSION     DAMJZE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.396"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>577
                     /locus_tag="PC541_003920"
     rRNA            <1..>577
                     /locus_tag="PC541_003920"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJZE010000380          541 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMJZE010000380 DAMJZE010000000
VERSION     DAMJZE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.397"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            293..457
                     /locus_tag="PC541_003921"
     CDS             293..457
                     /locus_tag="PC541_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841285.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT          209 a           39 c           84 g          209 t
ORIGIN      
        1 catatttgta tttttatgaa ctagaatatt ctttataaat tcatagcagt tttaatagat
       61 aactttttca gatagattaa acaaagtttt atttactgta ttctattatc agtttcctac
      121 aaattaaatt gtctattttt atttatagag tagaataaac tgtttttgtt aagaattttg
      181 aaggtaaagc tagtttaaaa aaataaaggg actaatttgt ctaacgttta ttggtgaaaa
      241 ataagtctat agaagttata ataaatatat aaaaatgtat gagaggtgtt atatgtttgg
      301 taaaccaaaa gaaagtgtta gatttttaga ctttaatgat gtagcagaaa agtttggaga
      361 gaaaaaggct gttgcaattt ttgatgccat agctggaaaa aaatctaaag aacatattat
      421 caaagatata gaaaaattag taaaaaatga agtttagata tttataaggt atattgagct
      481 tgagggtagc tctttatacc ttctttttta tgatttttag ataatatttt aaaatttaaa
      541 t
//
LOCUS       DAMJZE010000381          529 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.398, whole genome shotgun sequence.
ACCESSION   DAMJZE010000381 DAMJZE010000000
VERSION     DAMJZE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.398"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(153..452)
                     /locus_tag="PC541_003922"
     CDS             complement(153..452)
                     /locus_tag="PC541_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841286.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          200 a           70 c           54 g          205 t
ORIGIN      
        1 atattttata aactaatata ttttacaaat tgttgagtaa tattttatga tataaatcag
       61 aagaggcact tctataatag aaagtgctca actatgaata agattaataa tatatttgcc
      121 ttagatatat agacttttat aaatattaaa gattaatcaa atctgatgtt tgaaacttta
      181 tcccaattaa tcttccttga atagtcttct ggatgtgata atctaactat atgagttgaa
      241 ttatcatagc tgtaataaat tgtagtagta ccatcaactg gattcttttt gtatccttga
      301 tacacagcac tatttggtac tttcttttca acaaagctat atgcagatga agtatcaata
      361 gtttctattt tattaggaaa tataaacata taaaaagcta ctactataaa aatacatatg
      421 catgtaatac tataccattt aatccatttc ataaaattcc tcctacttta ccaaaatata
      481 ctattattat ttccatatat ttataatatt tatgcatacg ataaaattt
//
LOCUS       DAMJZE010000382          496 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.399, whole genome shotgun sequence.
ACCESSION   DAMJZE010000382 DAMJZE010000000
VERSION     DAMJZE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.399"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>496
                     /gene="tuf"
                     /locus_tag="PC541_003923"
     CDS             <1..>496
                     /gene="tuf"
                     /locus_tag="PC541_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2841287.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAMJZE010000383          493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.400, whole genome shotgun sequence.
ACCESSION   DAMJZE010000383 DAMJZE010000000
VERSION     DAMJZE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.400"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="PC541_003924"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="PC541_003924"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="PC541_003925"
     tRNA            310..398
                     /locus_tag="PC541_003925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="PC541_003926"
     tRNA            410..486
                     /locus_tag="PC541_003926"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAMJZE010000384          455 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.401, whole genome shotgun sequence.
ACCESSION   DAMJZE010000384 DAMJZE010000000
VERSION     DAMJZE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.401"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(134..421)
                     /locus_tag="PC541_003927"
     CDS             complement(134..421)
                     /locus_tag="PC541_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2841288.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
BASE COUNT          150 a           81 c           32 g          192 t
ORIGIN      
        1 cataaatttt ttggtatttt taataaaaaa caaaaaaaca gatatatact ataaatgctt
       61 ttacaattaa taatgtttaa ttaaagcaaa tataataaat atctgatttt tatatacaaa
      121 cttaattact tatctagtta agtccatttg attcatcttc gtcgtaattt aaaccttctt
      181 cttccattat agcagtatac acagctacaa cagtttcata ctcttcatca ctttccaaag
      241 gtattaagat ttcaccttct tcatcctcat caattctaaa gattaaagct tcttcagctt
      301 cttcatcatc taaaggcatt aatattgcat actcttttcc ttctgcttct aatgttaata
      361 ttatttcaaa ttggctttca acgccatttt cgtctattaa atttattata ttttcttcca
      421 taatgtcatt cacctcaaat ttatttttat tttta
//
LOCUS       DAMJZE010000385          425 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.402, whole genome shotgun sequence.
ACCESSION   DAMJZE010000385 DAMJZE010000000
VERSION     DAMJZE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.402"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..295)
                     /locus_tag="PC541_003928"
     CDS             complement(<1..295)
                     /locus_tag="PC541_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2841289.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACL"
BASE COUNT          142 a           79 c           53 g          151 t
ORIGIN      
        1 ctaaacaagc aagcttatga gcatctgcct gatcagtttt ccatgatctt agagcgctcg
       61 ttttaaattt ggcttctaac ggattcattt gaatatagtt aatttgattt acacaacaaa
      121 atcgttccat acctcttgaa tagatacctg tagattcaaa aatgagttgt gggtggtcta
      181 agtcattcaa atacttgagt aaataattgt aaccattttt attattctgg atgaaaaact
      241 ctttttggaa ttttccattt ttataatgtg caactacact acttctttta ctaatatcaa
      301 cacctaagta atcgataaaa aaacctcctt tgaataattg agaagctaaa aactttactt
      361 aacctttctc atttcatttt cctatacacg gtttcaagaa cccaacatac tacaaacgaa
      421 tttca
//
LOCUS       DAMJZE010000386          405 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.403, whole genome shotgun sequence.
ACCESSION   DAMJZE010000386 DAMJZE010000000
VERSION     DAMJZE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.403"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            1..405
                     /locus_tag="PC541_003929"
                     /pseudo
     CDS             1..405
                     /locus_tag="PC541_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          166 a           60 c           64 g          115 t
ORIGIN      
        1 aatcaacata ttaccgatgg aaaaacaaaa cccataaaaa tgataccgta acacaaaaag
       61 ttattgaatt atgtaaagct aaccactata cctacggtta tcgtaagatt acagcattga
      121 ttaatcaatg ttatacatca ccaattaatc ataagagagt acagagaatg atgcagaagc
      181 atcatttgaa ctgccgagtt agacctaaaa agacgacaag aataggtaaa ccgtattata
      241 aaacggacaa tttattacaa agacaattta aagcgagttg tccaatggag gtatggtatt
      301 aacaaccgat attacttatt taccatttgg tcattctatg ttgtatttat cttcgataat
      361 ggatatttat aacggagaaa ttgtgacata taaaatagat gataa
//
LOCUS       DAMJZE010000387          400 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.404, whole genome shotgun sequence.
ACCESSION   DAMJZE010000387 DAMJZE010000000
VERSION     DAMJZE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.404"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>400
                     /locus_tag="PC541_003930"
     CDS             <1..>400
                     /locus_tag="PC541_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2841290.1"
                     /translation="LNIYISEIDLIKAQLKKESTFNLVKVIEHYILKHRPEKYKQGEE
                     YYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAVNNYHKLLVDQKVGYSVGNPIVFNAD
                     DNDFTKLLNDLLGEEFDDTITELYLNASNKG"
BASE COUNT          167 a           45 c           70 g          118 t
ORIGIN      
        1 gttaaatatt tatataagtg aaatagattt aataaaagct cagttaaaaa aagagagtac
       61 ctttaaccta gtaaaagtca tagaacacta catcttaaag catagaccag agaaatacaa
      121 acaaggagaa gaatactatt atggtaatac tgatgtaaac aataagagaa ggtattatct
      181 cttagatgga gctaaggttg atgattttac taaggttaat aataaagcag ttaataatta
      241 tcataagctt ttagttgacc aaaaagtggg ctatagcgtt ggaaatccaa tcgtatttaa
      301 tgcagatgat aatgatttta ctaagctttt aaatgattta ctaggagaag aatttgacga
      361 tacaataaca gaactctatc tcaatgctag taataaaggg
//
LOCUS       DAMJZE010000388          394 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.405, whole genome shotgun sequence.
ACCESSION   DAMJZE010000388 DAMJZE010000000
VERSION     DAMJZE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.405"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT          147 a           55 c           24 g          168 t
ORIGIN      
        1 tagtatcatt ttagtatcat catttattta atacattatg tattccgttt agatttagtt
       61 atccgaaaac ttagataact agcttgaaat ttctacagat tattacatac tttattgtaa
      121 ttatatcata atgttataat tacatattat caaattacac actatctttt attcatatag
      181 atattatcat ccttgtgtat atattgtcaa agaaaacatt taatttacaa catattttat
      241 atcatatcat ctaaactatt atatacacct tatctaattc acacattaac actattgtac
      301 tttttctaaa aatttttaaa taaaaatgac agctttaatt tattatttta aaactgccat
      361 taaactgtaa atattccctt ttagtataca aaat
//
LOCUS       DAMJZE010000389          393 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.406, whole genome shotgun sequence.
ACCESSION   DAMJZE010000389 DAMJZE010000000
VERSION     DAMJZE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.406"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            64..345
                     /locus_tag="PC541_003931"
     CDS             64..345
                     /locus_tag="PC541_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841291.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          173 a           26 c           65 g          129 t
ORIGIN      
        1 aaagatattt ttaagaataa aatttattat taaataaaaa ataaaggaga gtgatttaat
       61 agcatggcat caaaacaaat accaagaaac aatttagaaa agtttttaag aataagagtc
      121 ttatcacata caggcgataa aatacatata aagttacctg taaattttgc aaagagaatg
      181 attgagaata atgctttgga tttatttaat ggaaaagatg atgtagtaga tggtaaaaaa
      241 gttactgaaa tattagttaa ggcatttgac tatgatttaa ctggagaagt agttcacttg
      301 gagagaaaaa atggagacat tattaaagtt tcaattgtac aatagaatta ttttatatga
      361 attttatttt tgaatagtta taaataatta gaa
//
LOCUS       DAMJZE010000390          389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.407, whole genome shotgun sequence.
ACCESSION   DAMJZE010000390 DAMJZE010000000
VERSION     DAMJZE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.407"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT          167 a           34 c           38 g          150 t
ORIGIN      
        1 gtcattaaat atgccaatag ttttatatat tattgatttt attaaaaaaa gagatataca
       61 aactaataag ttgtaatctc tcaaatataa aaaaacaata aattagctca ttggattttt
      121 tagtccataa tataattata gattattcac caaacaatct atagaaattg tggtattaat
      181 gtttaaataa ccaacttttt ctaaaataac ctttatagga atatatgagt tcataagtaa
      241 agatagcgaa ataagaaacc agtttggtat gtaaaatcta gatttttaat taaattatca
      301 agattatata ttatagtatc aaaaatagaa aattaaaatt aaatttttta taataaatct
      361 tatttttggc taacaagatt tattttttt
//
LOCUS       DAMJZE010000391          384 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.408, whole genome shotgun sequence.
ACCESSION   DAMJZE010000391 DAMJZE010000000
VERSION     DAMJZE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.408"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            144..332
                     /locus_tag="PC541_003932"
     CDS             144..332
                     /locus_tag="PC541_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841292.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
BASE COUNT          160 a           27 c           62 g          135 t
ORIGIN      
        1 ttattaaaat atgtaatgtc actgatagtt agaatattta agtgctaaaa gatgtgtata
       61 aataataatg atgtcagttt ataatgatat taaattgaga tatactgttt ctctaaatta
      121 aaaaataaag gagtaaatgt gttatgaatt atcatacaaa agaagaattg gtggaagcac
      181 tgagagtagt atcttcaagt attattaatt gtggaaaagg acagaagaaa ttttctgagg
      241 agacatctca tcatacacgt tttaagaata ttatagaagt aatgtatatt tcaaaatcat
      301 taataatgga tgaaattagt aaaagagatt gaagcttatt ttgcaattta acatgtttta
      361 atatacattt tattatataa gaaa
//
LOCUS       DAMJZE010000392          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.409, whole genome shotgun sequence.
ACCESSION   DAMJZE010000392 DAMJZE010000000
VERSION     DAMJZE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.409"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            155..>364
                     /locus_tag="PC541_003933"
     CDS             155..>364
                     /locus_tag="PC541_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841293.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAG"
BASE COUNT          148 a           36 c           80 g          100 t
ORIGIN      
        1 acagtatatt cattgacaaa tataagtttt aagtatatat taaaaattat tagagtgtgc
       61 attagaatgt atacagcgta tattaagaat tattagagtg tatataagga tgtatacagt
      121 gtatattaaa aattattaag gggaggggga gtttatgcaa caaaaatgta aaaagaaagc
      181 actatcaatg attttaagta tctcaatggg acttggacca gtagcatcat ttaatgcgtc
      241 aaatactttc gcagatacta tagaagaaag tactaatgca ggaaatgcag aaggaggtac
      301 taatgcagga aatgcagaag gagatactaa tgcaggagat gcagaaggag atactaatgc
      361 agga
//
LOCUS       DAMJZE010000393          363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.410, whole genome shotgun sequence.
ACCESSION   DAMJZE010000393 DAMJZE010000000
VERSION     DAMJZE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.410"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="PC541_003934"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="PC541_003934"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="PC541_003935"
     tRNA            290..361
                     /locus_tag="PC541_003935"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAMJZE010000394          349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.411, whole genome shotgun sequence.
ACCESSION   DAMJZE010000394 DAMJZE010000000
VERSION     DAMJZE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.411"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT          138 a           36 c           31 g          144 t
ORIGIN      
        1 acctttttaa ataactgtcg atttattaaa gttatctatg tactatatta aacaattaag
       61 tagttattaa ttgaacttat tagtttatta aaaaactatg tatattactt tgcagtaaga
      121 ctttggaact aaatctataa tagcaaattt aaatttatta aatagattac cttataacat
      181 ttcatataaa atatattgtg aatctgagaa aattactatt tttctttgag tattttttgt
      241 aagcagttct aaaagattca atttcatacc tattacttga atcattcatt aaaataattc
      301 taatttaaaa aataattcta tccaaataat agatatatag gactatgtt
//
LOCUS       DAMJZE010000395          330 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.412, whole genome shotgun sequence.
ACCESSION   DAMJZE010000395 DAMJZE010000000
VERSION     DAMJZE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.412"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>330
                     /locus_tag="PC541_003936"
     CDS             <1..>330
                     /locus_tag="PC541_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2841294.1"
                     /translation="EGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFG
                     FNHANVVLRIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRI
                     KMWKSAGY"
BASE COUNT          126 a           32 c           73 g           99 t
ORIGIN      
        1 gaagggtaca aagtctatgg tcttggagaa tggggagaaa ctggtggagc aatacttaaa
       61 aattatgtta tacatgaatt tcctacagaa tttgagtatt ttgacaatat gaggttatca
      121 caagactttg gatttaacca tgcaaatgta gtacttagaa ttggctttaa ggatggagaa
      181 ttatatatat gtaacgaaat atatgtacat gaaatggata cctcagaaat cataaagatt
      241 gcaaataata aaggtttaga aaagaatcta tttatgtact gtgatagtgc tgaaccagat
      301 agaattaaga tgtggaagag tgcaggatat
//
LOCUS       DAMJZE010000396          315 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.413, whole genome shotgun sequence.
ACCESSION   DAMJZE010000396 DAMJZE010000000
VERSION     DAMJZE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.413"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>315
                     /locus_tag="PC541_003937"
     CDS             <1..>315
                     /locus_tag="PC541_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2841295.1"
                     /translation="EGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFG
                     FNHANVVLRIGFKDGELYICNEIYVHEMDTSEIIKIANSKGLEKNIFMYCDSAEPDRI
                     KMW"
BASE COUNT          119 a           30 c           70 g           96 t
ORIGIN      
        1 gaagggtaca aagtctatgg tcttggagaa tggggagaaa ctggtggagc aatacttaaa
       61 aactatgtta tacatgaatt tcctacggaa tttgaatact ttgataatat gagactgtca
      121 caagactttg gctttaatca tgcaaatgta gtactcagaa ttggttttaa ggatggagag
      181 ttatatatat gtaatgaaat atatgtacat gaaatggata catctgaaat aataaagatt
      241 gcaaacagta aaggtttaga aaagaatata tttatgtact gtgatagtgc tgaaccagat
      301 agaattaaga tgtgg
//
LOCUS       DAMJZE010000397          302 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.414, whole genome shotgun sequence.
ACCESSION   DAMJZE010000397 DAMJZE010000000
VERSION     DAMJZE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.414"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT          102 a           32 c           28 g          140 t
ORIGIN      
        1 atttatataa atattttttg tttttaattt tttctatctt cttatgtact tacatttaat
       61 ttattctttt cagttaataa aataggtaca tttttagtac atgaaaatag agttgcttac
      121 agggtatata ggattgatgt actatttact aatattacat tttcaactga ttcaacccct
      181 cttttactta cataatttaa gtatattttt ctggattttt aaatttatgt actttaaaat
      241 ggatatactt attttcattg attttgtaag aattaataaa aaatacgatt acactacaaa
      301 at
//
LOCUS       DAMJZE010000398          269 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.415, whole genome shotgun sequence.
ACCESSION   DAMJZE010000398 DAMJZE010000000
VERSION     DAMJZE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.415"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT           96 a           35 c           22 g          116 t
ORIGIN      
        1 tatgtaattt tttaatctta ttttattcca tctatactct tatgctaagt aataactttg
       61 ataatatttc cctaacttat gtttcaactg gtttatattg caatttattt aattacagct
      121 tatttgcata ataattcgtc catatcataa gttgtgtatt tatataaata cacaattaaa
      181 aataaatcac tatggtatac taattattac gaagactata cttatatgca aagacaaata
      241 taaggcatct tatatctttt attaattat
//
LOCUS       DAMJZE010000399          248 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.416, whole genome shotgun sequence.
ACCESSION   DAMJZE010000399 DAMJZE010000000
VERSION     DAMJZE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.416"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>248
                     /locus_tag="PC541_003938"
     CDS             <1..>248
                     /locus_tag="PC541_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2841296.1"
                     /translation="EHYILKHRPEKYKQGEEYYYGNADINNKRRYYLLDGAKVDDFTK
                     VNNKAINNYHKLLVDQKVGYSVGNPIVFNADDDNLTKL"
BASE COUNT          101 a           31 c           43 g           73 t
ORIGIN      
        1 gagcattata ttttaaaaca tagaccagag aaatataaac aaggtgaaga atactattat
       61 ggcaatgcag atataaacaa taagagaaga tattatctct tagatggagc taaggttgat
      121 gattttacta aagttaataa taaagcaatt aacaactacc ataagctttt agttgaccaa
      181 aaggtaggct atagtgtcgg aaatcccata gtatttaatg cagatgatga taatctcact
      241 aagctttt
//
LOCUS       DAMJZE010000400          234 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.417, whole genome shotgun sequence.
ACCESSION   DAMJZE010000400 DAMJZE010000000
VERSION     DAMJZE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.417"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>234
                     /locus_tag="PC541_003939"
     CDS             <1..>234
                     /locus_tag="PC541_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2841297.1"
                     /translation="NITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNV
                     LEVFDKLMEKMTEARVPENGRILYVTPVVNTLI"
BASE COUNT           97 a           32 c           42 g           63 t
ORIGIN      
        1 gcaatataac aaaagtatat aatgaggaac aaaagtttcc agagatggat gcttactgta
       61 tatctaaaat atatgctgat tggaccgcat taggtaacac agcagataca actgttctta
      121 caacaacaaa cgtattagaa gtatttgata agttaatgga aaaaatgaca gaagctagag
      181 tacctgaaaa tggaagaata ttgtatgtta ctccagtagt aaatacactt atca
//
LOCUS       DAMJZE010000401          232 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.418, whole genome shotgun sequence.
ACCESSION   DAMJZE010000401 DAMJZE010000000
VERSION     DAMJZE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.418"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..>232)
                     /locus_tag="PC541_003940"
     CDS             complement(<1..>232)
                     /locus_tag="PC541_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2841298.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAMJZE010000402          213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.419, whole genome shotgun sequence.
ACCESSION   DAMJZE010000402 DAMJZE010000000
VERSION     DAMJZE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.419"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
BASE COUNT           91 a            7 c           32 g           83 t
ORIGIN      
        1 attataaatt atgtattgat tatatattga gtaaagtatt ataaagcatg tattgattaa
       61 gtatttatta agtattgaat aaaatattac aaatttagtt gaaatgaaaa tgataaaaaa
      121 tgttgtacta tattttacaa atcatataaa tagagaaatt gttgtagcgc aaagtagtag
      181 gtagtatatt agaatataat tataggattt gtt
//
LOCUS       DAMJZE010000403          213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.420, whole genome shotgun sequence.
ACCESSION   DAMJZE010000403 DAMJZE010000000
VERSION     DAMJZE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.420"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(<1..>213)
                     /locus_tag="PC541_003941"
     CDS             complement(<1..>213)
                     /locus_tag="PC541_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2841299.1"
                     /translation="SLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTA
                     IIVGSFIYQFTIYFAMSLGMLSTDLKL"
BASE COUNT           83 a           33 c           31 g           66 t
ORIGIN      
        1 tagttttaag tcagttgata acattcctaa actcattgca aagtatatag tgaattggta
       61 tataaaagag ccaacaatta ttgcagtagt atctttgata aaagtaaatt tcttaaatag
      121 tgtaatacca attatgatag aggcaattcc aagaactaaa gttcctatac ccatatttac
      181 atctgagaat cccaagaact gagccataag act
//
LOCUS       DAMJZE010000404          212 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.421, whole genome shotgun sequence.
ACCESSION   DAMJZE010000404 DAMJZE010000000
VERSION     DAMJZE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.421"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            <1..>212
                     /locus_tag="PC541_003942"
     CDS             <1..>212
                     /locus_tag="PC541_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2841300.1"
                     /translation="PNVDRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYF
                     ENIHSIPGIGKLSTAMIIGEIGDIKR"
BASE COUNT           73 a           37 c           32 g           70 t
ORIGIN      
        1 cctaatgtcg atagacattc ctttctagtc gaaaaattac gcttacttat tcaacaatta
       61 aaacaatcta ttcatcatct caaacaatta gatgatgcca tgattcaatt agcacaacaa
      121 ctcgattatt ttgaaaatat tcattcgata cctggtattg gtaagctaag cacagctatg
      181 attattgggg agattggtga tattaagcga tt
//
LOCUS       DAMJZE010000405          209 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.denovo.422, whole genome shotgun sequence.
ACCESSION   DAMJZE010000405 DAMJZE010000000
VERSION     DAMJZE010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.denovo.422"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            73..>209
                     /locus_tag="PC541_003943"
     CDS             73..>209
                     /locus_tag="PC541_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2841301.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT           91 a           25 c           32 g           61 t
ORIGIN      
        1 taaatatttc actaaaaaaa aacaaatgca aagctagtac caaaaataaa tcagaaaaaa
       61 gaggtgttgt agatgttaca aattaaaaat ctttcaaaga gtttccctaa tccatatggt
      121 gaacctaata ctatctttga aaatttatca atcgatatag aagatggtga atttgtaagt
      181 atcataggta gtaatggaac tggtaaatc
//
LOCUS       DAMJZE010000406        17169 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.guided.082, whole genome shotgun sequence.
ACCESSION   DAMJZE010000406 DAMJZE010000000
VERSION     DAMJZE010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.guided.082"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            complement(230..754)
                     /locus_tag="PC541_003944"
     CDS             complement(230..754)
                     /locus_tag="PC541_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2841302.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(819..1571)
                     /locus_tag="PC541_003945"
     CDS             complement(819..1571)
                     /locus_tag="PC541_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2841303.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1602..2900)
                     /locus_tag="PC541_003946"
     CDS             complement(1602..2900)
                     /locus_tag="PC541_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2841304.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2917..5181)
                     /locus_tag="PC541_003947"
     CDS             complement(2917..5181)
                     /locus_tag="PC541_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2841305.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5436..6254)
                     /locus_tag="PC541_003948"
     CDS             complement(5436..6254)
                     /locus_tag="PC541_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2841306.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6247..6945)
                     /gene="cbiQ"
                     /locus_tag="PC541_003949"
     CDS             complement(6247..6945)
                     /gene="cbiQ"
                     /locus_tag="PC541_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2841307.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6948..7232)
                     /locus_tag="PC541_003950"
     CDS             complement(6948..7232)
                     /locus_tag="PC541_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2841308.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7222..7974)
                     /locus_tag="PC541_003951"
     CDS             complement(7222..7974)
                     /locus_tag="PC541_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2841309.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8156..8348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8480..10252)
                     /locus_tag="PC541_003952"
     CDS             complement(8480..10252)
                     /locus_tag="PC541_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2841310.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10266..11195)
                     /locus_tag="PC541_003953"
     CDS             complement(10266..11195)
                     /locus_tag="PC541_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2841311.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11416..11601
                     /locus_tag="PC541_003954"
     CDS             11416..11601
                     /locus_tag="PC541_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2841312.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11594..12295
                     /locus_tag="PC541_003955"
     CDS             11594..12295
                     /locus_tag="PC541_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2841313.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12297..13220
                     /locus_tag="PC541_003956"
     CDS             12297..13220
                     /locus_tag="PC541_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2841314.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13377..14300
                     /locus_tag="PC541_003957"
     CDS             13377..14300
                     /locus_tag="PC541_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2841315.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14293..15030
                     /locus_tag="PC541_003958"
     CDS             14293..15030
                     /locus_tag="PC541_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2841316.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            15041..15814
                     /locus_tag="PC541_003959"
     CDS             15041..15814
                     /locus_tag="PC541_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2841317.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16355..16963)
                     /locus_tag="PC541_003960"
     CDS             complement(16355..16963)
                     /locus_tag="PC541_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2841318.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5545 a         2971 c         2164 g         6489 t
ORIGIN      
        1 atacattatt atttaaaaat aaatatataa acaatcataa taaaattttt ttaataaaac
       61 tttatttttt agttaaaatc atacaatata tagatttcca caatacaaat aaattaaaaa
      121 gtgaaatgta tttgtctaaa aacataataa catttcactt tatcctcatt tttaagtaat
      181 atatattttt aatttttatt tataactaaa tctagttttt atataaattt tagttttttt
      241 ctacatctga gtaatctatt ccaaaagttt ctacagtaac agatttcata acttgtggag
      301 tttgtggctt atcagctgaa tctgttctaa ctgatgctat ttcatcaact gtatccatac
      361 cttctatcac tctaccaaaa cctgcatact gaccatctaa gtgtggtgag tttttatgca
      421 ttatgaaaaa ttgagaacca gctgaattag gtgccatagt tctagccatt gatatgactc
      481 ctctttcatg ttttaaattg ttagtaaatc cattaccact aaattcacct tttattgagt
      541 gacctggacc acccattcct gtaccatttg ggcatcctcc ttgtatcata aatcctggta
      601 ttactctatg aaatattatt ccattgtaat atccttcatt tactaatgat acaaagtttt
      661 taactgtatt tggagctata tttggatata actcaatttt gatttctttt ccattttcca
      721 tttctatagt tactatagga tttttatttt ccataattta ttaccttcct tttcattagt
      781 taaattacat aattacattt attatattat actctaaatt aaagtctatc aagtatattt
      841 tttcctattt catatccttc ttgataaata gttttacaaa actcatcatc aaagttaagc
      901 gtgtctttag gagcaaattg agtattaggt ctaactacta ttattttatc ttcatcatat
      961 acttttttaa tatcttctgg caactcataa ttatattttg tggatattaa aataatttta
     1021 tccacattat ccccaattaa tggttcaatt aatgcactcc taattaaacc tccatctaat
     1081 ttgaaaccat cataaattcc ttcttgcaca tctttcacaa attgttcttt aaatccaaga
     1141 gttgcgtttg gatttgatgg taacaaccca ctgtatgaaa tgctgataat ggcttcatta
     1201 cgaggcattt ttgatacgtc aacaatcttt tctgatacta ctttattatc aacttcaaca
     1261 taattaacat aaaagttcat ttcttctata ttactatcat ctagttccct taggttgtct
     1321 atagcaggag aattatccac agtattattt actattttta catttccatt ctggatatct
     1381 tttatatttg tccacatttt ctccaagtta tcaacattcc ctgtgtacaa ataatatcca
     1441 tttattgctc ctattgatgt ccctgaaatc gaataaaatt tatttattcc tccatcatac
     1501 aaggctttaa ctactcctgc ttgataagcc ccttttgctc ctccaccttc taaacataat
     1561 cctaatttca tagtctcatc tccaaattgt ttatcttata tttatatttc aaaaaactta
     1621 ctaaatgttt tatatatact atagtggtcc tcaacatttc ctaattctct tatagaatgc
     1681 attgctaaaa ttggacttcc cacatccaca gatggtatat ctatatgagt agaagatatt
     1741 ggacctatag tacttcctcc tctttcatct gacctattaa caaattcttg atactcaaca
     1801 ttagcttctc tacatatatt tttataaata gctatagaat atccatcact agtatatgcc
     1861 tgactagcat ttatctttat aaccggacct tttcccatta ttggtttatt agttgggtca
     1921 tgcttttctg gaatgtttgg atgaatagca tgagctaaat ctgatgatat cataaaagat
     1981 gaataaatag actcaaagaa tccctctcta tccttaccca ttgatatgca aattctctcc
     2041 aatatattta acaacatgtt tgaatctgca ccttgtttag tagagctacc aacttcttca
     2101 ttgtcaaata cagccattac atttacacca tgttgacctt tagtattaac aagagcattc
     2161 aaactagcat gtaccattgc aagattatct tgtctcccaa tagatataaa ttcatcatta
     2221 gcccctatta aacttccttt ttcaaattca tataaaaata aatcaaaatc tataatatcg
     2281 tccatattta catctaagtt agatgctatt tgctttataa gaaaattatc tttttctaat
     2341 gtatcattta acatcccaac taaaggtaac atatcttttt gcttattata tgagtgacca
     2401 tcatttatac ttctatttaa atgtattgct agatttggta ttatacaaat tggcttttta
     2461 aagtctataa gcctttcatt tggttttaat atactatcac cttttaaaac tactctacca
     2521 gctatcccaa gaggtctatc caaccatgta cttaatattg ctcctccata gcattctgta
     2581 tttaacttca aataagattt ttcagcttct atttctgcat ttggtttaat actaaatgta
     2641 ggagaatctg aatgagagcc tattattcta aatccatctc tttctatacc ttctgaattt
     2701 actacaaaag ctactatagc tgataaattc ttagtaacat aatactttcc accaactctt
     2761 agattccatc tttgattcaa aactaattct tcaaatccat ttttatctaa gatttcctta
     2821 gtactctcga ctgcatgaaa agacgacgga ctatcatata tgaagtctat taagttttta
     2881 gcaaagttta tactatccat tcctcattta cctccattat aaaatattta gtgttgtata
     2941 ttctttattt atttttttaa ttccctgttg gtcaaaagct atagtaacat ctgtaccaat
     3001 tatagaaacc actgtcccaa ccccaaattt aggatgatgt accttagaac caagttttat
     3061 atcatctgga ttagtttctt tattagaatc ttttattgta gcattaactt tatctgcaac
     3121 tttagatttg cttatagtac tcatatactt tttagtatat ttatctaaca tattataact
     3181 tgctttagaa tagcttagtt ctttttttac tttgtacaat ctttctatac attcttctgg
     3241 cagttcttcc ataaatcttg aagctattgc aacatttgtt ttaccatata atgttctctt
     3301 ctctgtcaat gacatatata gctcttcttt agctctagtt ataccaacat aacaaagtct
     3361 tctctcttct tctatttgtg aatcactcat agattttata gctcttgata taggaaataa
     3421 cccttcttcc atacctgtta aaaacacaac agggaactct aaacctttgg atgtatgaat
     3481 tgtcataagc gaaaccctat cattgtcttc ttcatcattt gattcagatg taagtgctat
     3541 acttgttagg aatgtctcta agtctttatt ttcataagta tcatcattac tactttcaaa
     3601 ctccaaagct atagaaataa attctctcaa attatcaatt ctgtcttgag cttcttcacc
     3661 tttgcctgtt aaatcttttt catttttatt tcttatctct acaagctcgt ccatatatcc
     3721 tgtagtatcc aagacttttt ctataagttt gctaacagga taaacttctt ttatagttct
     3781 aagagtccct ataatatcaa caaatccact tatacttgcc cttgccttag tagatatatc
     3841 tgaatttgtt tctatatcaa ttaatactga gtatatactt tcctgtttta agttagctct
     3901 atcttcaatt ttttctatag ttctaagccc tatacctctt ctaggaacat ttataatcct
     3961 ttttatagat atatcatctt gtggattttg aataactctt aaatacgcca acaaatcctt
     4021 tatttctttt ctctcataga attttgtacc accatatata ttgtatggaa tttgacttct
     4081 atttaaagcg tcctctacag gtcttgcctg tgcatttgct ctataaagta ctgcaaaatc
     4141 cttataaggt ctattttctt ttctagctat ttgtgcaatt gaatcagcaa tgaaatcagc
     4201 ctcttctatt tcactaccag ttaattgtat ctttataagt tctccttctt ttttttcact
     4261 ccaaagcttt tttctttttc tttctatatt attagatata actttattag ctgcatctaa
     4321 tataacttgt gtagacctat aattttgttc caactttaca acatgtacat tgtcataatc
     4381 tttctcaaat tcaagtatgt ttcttatatc tgcacctctc catccataaa tactttggtc
     4441 atcatctcca acaacacaaa tattttgatg ttctcttgct aataatttta tcaattcata
     4501 ttgagcttta cttgtatctt ggtactcatc taccattatg tatctaaatc tacttctgta
     4561 atatgctaac acttcatcat tagccttgaa aagttctaca gttttaagta ttaaatcatc
     4621 aaagtcaagt gcactatttc tttttaaacg gtcttgatat agcgcataaa tatctgcaat
     4681 cttaaccatt ctattatctg ccatattgat atctttaaac tgctttggag tataaagttt
     4741 gtcttttgct cctgaaattg cacttataac tgcttttggt tcaaacactt tatcacttaa
     4801 atttaattct tttagacagt ctttaaccaa tgtaacttgg tctgctgaat cataaattac
     4861 aaaacttcta ttgtaaccaa tcttatttat atcttttctt agtatacgaa cacagcaaga
     4921 atggaatgta cttatccaca tatccttagt ttcaggacct atattttgtt caactctctc
     4981 tctcatttca tttgcagctt tgtttgtaaa tgttatagct aatatatttg gtgcttgtac
     5041 acctttatct tctataagat atgctattct tgttgttaat accttagttt tacctgaacc
     5101 agcaccagcc agtataagta ctggaccttc agtcttttct actgcttctc tttgagcagg
     5161 atttaatgta tctaaattca tatttctcct cctgttaaca gccataaata tattttaatc
     5221 taaaatatga attacttcaa attataatta tgtattatat taatagatta tttccaatat
     5281 attacaatta aataacattt ttccatataa tttatttcat tgtttaataa aaaaaataac
     5341 cttatgattt ggggaatcat aaggcacctt taaggggtct aggttaaggg ttattatata
     5401 taaatctatt tttttaaaat aggtttaggg cttaattatt tatttgtatt aagttctctt
     5461 tccttccaat acttatacaa atcttcttct ttcttaaata agggtaaatt catatttcta
     5521 tgtactttta ctaaccatgg ggattctagg ccagcttgta taatattatt ttcatttata
     5581 aacacatttt ctgcttttcc ttcatcaatc aaaatcccct tatccaatac atatatgtaa
     5641 tcacatattt catacattaa attcatatca tgactggata ttactatttt tatattattt
     5701 ttatttaatc cctttattat atctacaata gacctagtac ttactgggtc taaaccagct
     5761 gtaggttcat caagtaatac aatttcattt tccattgcta taactgatgc tattgccact
     5821 ctttttttct gtccatagct aagaaaatgt actggcctgt ctataaaatc tattccatta
     5881 acagctacta gagctttgtt tattctctct ttaattgttt tttcatccat accaatgttt
     5941 ctcattgcaa aggctatatc atcatatact tttgaataaa aaatttgttt ttctgggtct
     6001 tggaagacaa tacctacatt ttttctaagg ttgtataaac ttctcttatc atattttaac
     6061 ttttcttctt taaaaagaat ttctccagat gtaggcttta atatacccat taaattcata
     6121 aataatgttg attttcctga accatttgaa cctattatac ctataacatt tccctttgaa
     6181 aaatccatat taatgtttaa tagtgcattg gtattttttt catattgata cgttaaatta
     6241 tttattttaa acatcattat cccctactat gtgaaattta ccatcataca attttatgtc
     6301 taatgatata cacatatcat cataccttgt cataagtctt ttgtacagca tacttccaag
     6361 aatacctaag gatttatatg aagtttttaa atttatatat ccaaatctta gctcttgtga
     6421 cttgtatatt tctgaaaatt cttctaaaaa tatgaatata aacctatata ttaacattga
     6481 taactcgaca aaagtgtcag gtaaatgaag ttttttaaaa aagaatatta attgattaaa
     6541 aggtgttgta agtatacaaa aatacacaca agtcaaacaa gacatcgctc taaataacac
     6601 atgaatagac atgttaatag acgcctttga tattcctaca ttaaaactaa aaaattcaaa
     6661 actatataat aaatctgctt tattaaaaga tatatttaca agagttattc ctatactaag
     6721 aaataaaaag tacattggta ttctaagtaa ttttaaataa ttttttaaat ctattccagc
     6781 cacacaaaca accaaaatac tcattaaaat cataattcca cttaaaataa atatattttg
     6841 tattaccata gaagcaatca gaaaaattac tcctatggct acttttttgt ttggatttac
     6901 tttactcaat ctatttgtat atgcatattt atcaattata agcatgatta acttctcttt
     6961 cctttttgaa gacctaatat gtatcctatt acaccagccc ctaaagctgc ttgtgcagag
     7021 aataataaac tttctatttc tccacttggt ggctcatatg gactgctaaa ccatggtttg
     7081 taatttggat ttatcttagt aatttcactt tcagcttgac catctgctcc tccatattca
     7141 gcacctgagt tgacaagtaa tgggaatata ataagagcta ctactaataa aagtaataat
     7201 atgttagttt ttgtttttgt tttagcactc attagcttta actcctttat cattattttc
     7261 tagtatgctt ttttctccgt tttctgtaat taaattatat acaattacag taagtatacc
     7321 ttctgctatt gctattggta tttgagtcat aaagaaaatt cctaaaaatt taattgctga
     7381 agccattacc ccacctgatg ggtctgggaa agccagtgct aattgtattg aagttattgt
     7441 atatgtggct aagtctccta ttgctgctgc aagaaatact ggaatagcat tatttccatc
     7501 ttttttcttt aaagccttaa atattaggaa tgatacgatt ggccctatta tcgccattga
     7561 aaaagcatta gctcctaaag tagttattcc tccatgtgct aataacaatg cttggaatat
     7621 taaaactata gttccaagaa caaacattac gcttggtcca aacattattg ctccaagacc
     7681 tacaccagta ggatgtgaac aacttcctgt tactgaaggt atttttagtg ctgataatac
     7741 aaacacaaat gctccacata aagctaataa tactttcttt ttagggtctt gctttactat
     7801 tttacctatt gattttaatc cgaataagaa aaatggtata aatataactc cccatgctat
     7861 agaccatttt actggtaagt aaccttccat tatatgcatt gcatttgatg taactggagt
     7921 taaaatgatt aacataagtg cagctataac ccctaattta atattctgtt tcatcttagt
     7981 atccctctct ccctttctaa taattaaaac ataataatac atgtctgatt tatttaataa
     8041 aataaaaaag cctaactcga tggttaggct tctaattttc cacaatctaa atacaaacac
     8101 ataaaacaaa catatctgta taattattat aagaaacttt ctccatcccc gaagaaatta
     8161 ctttaatatt ttaggcaggt ctcctgactt agcttcatcc tactcctttt ccttcccatg
     8221 agatataaaa aatatcatca tagtggctaa caaaagtttc gtctgcatca cagtagcggg
     8281 ggctgtaaag gaatttaacc tttttcccta ttaatcttgc ttaaagaacc tataatatta
     8341 tatatttaat ttttaaatta ttcttaaatc attttctata taaatcattg tatacctttt
     8401 ctgtgaaaat ttctacattt tttataaatt ttattcattt ttatattttt atgtatataa
     8461 attctttttt gtaagtttat taatttctaa ttatcttctt taaatacatt gaaactacat
     8521 cagaaaatct agttatatct cttgaatata taaaatcatt tccatatatt aatctttcag
     8581 cttcaagttc tgattctttt cctgtaaata ctcctaatac taatatattt tgctttctag
     8641 cttttctcac ttcattggct gtatctctca aaccaactat acccctataa gccttttctc
     8701 ctcttaatgt tctctcacta ctttttccta tctttacatc atttggtctt ccatcgctga
     8761 gaactatcag gattttattt tcttcatctc tatttattag accactacat atagatttta
     8821 ttgccaaacc atctctatta ttaccagcac aaaaatattc aaatatattt tcactatttt
     8881 ttatactatc atcataatct ttaaatcttt ttaatattgt atagtctaaa aaactagaaa
     8941 atcccatcac tctatttggt atgccaactt ctgttagagc atttgcaatt atgtaagctt
     9001 gaattgccac atttccctga tttctacttt gagagccact tgcatccaat aatatatcaa
     9061 taacgtattt ccctttttca ttttctatat ctttataaaa tattttatta ttattacttc
     9121 taccaactct ccaagctcta ttggctaata tagtaccaca atcagaatat actctacttt
     9181 tagcattttc ttctattaaa attcttgaca gactttcttt taatctcttt atttgctgtc
     9241 tatacattct agcatgttct ctaaattttc ctaaattatt ttctttttgt cttgttacat
     9301 attttagttg aaatacatta ttacactcac ttctaagaac tccatctgta aagtgtattc
     9361 tacaaccttc atgaacattt ctacaatttt gtgtctcaat ttttttaatt tcacctttac
     9421 tcaaataagt ttttccataa taatgttcta tttttgaata tatcttattt gcagtgtctt
     9481 catcaacata tataactcgg tctgctgaat tgctacattt agaatcatca aaatccccta
     9541 caccttctac aagcatggat gaagatattg aatctatatc actttcaata atattttctt
     9601 cttcatcact ataaagttct tcatacatga aatcagcaaa tgtatcaaaa tttacttcta
     9661 cactttctac attattttca tcaataagac tattttcttc tccatttaat atgtattcaa
     9721 aataagttaa atatataaca tctaatgctt ttaaaatctc cataatatct tgattggtat
     9781 ctatatttct tatatcatta actattcttc tagtcatact atctaatgct gggtgcttgt
     9841 ccatactata aagtacaaat gtgtatttaa ttttatccat aatattatca ttgtgtattt
     9901 tggtaaaacg tgaaacaata tctttataag cattccttct tatgtcttct atccctggcc
     9961 tgtctttaat gaccttttct tcaactattg tatttaatat aacttgaatt agacctagta
    10021 taacatcttt gttgtatcct tttcttattc tatttgtaag atatttttta actatatccc
    10081 aatctacata ctttcttcta gcacctgcaa ttattgcgaa atatagacct atttcttttg
    10141 aagagtaatc tttttctgat atatctatac tttcattgta atcaccagct attgtccatg
    10201 ataggttatt agctctattt tcaaattcat aatcatcata tatccatttc ataatctcac
    10261 ctatattaaa tatgacttat tggaaacaca tctatactct cccatttatt aggtattcta
    10321 gttttaatta catctccaac catttctttc tcatatacat caaaagtctt tcctgtaagt
    10381 cccatattaa ttgcaagagt tggttttaat cctcttctta tagtcttaag agatgccata
    10441 agaccacgta aatcaatagc tttactagat atctctccat tttgagactt caattgtaaa
    10501 tctaaaaata ttcctgcaaa gtgtattaat ttttcttcat cagcatctga aaattcattt
    10561 tttaaaatca tcattaactt atcttcttct ataggaggaa tatctattac cataaatcta
    10621 gataccagtg cttcattaag ttcttttgtt cctgcatatt cataattcat agtcccaata
    10681 aatcttgttg ctggatgtag attgactctc tcatacccag gtacatcaat tattcttcta
    10741 tagtctaaag ctgaatgtac cacaactata gcatcatttt ttgccatatt aatttcatca
    10801 aacaccccaa aacctccatt aatagcacac tcataaactg agcctcttct aagtttaact
    10861 tcattgtcta taaatgtatc tgtacctata agtgttgaac tgtctgtatt tatatgaaat
    10921 gatgtattcc actgaggtct attaaatatt tcccctagat tatcagcaag taagtttttt
    10981 cctgttgcct ttggtcccga taataatata ttttcttctt ctaaaatagc tgctatacac
    11041 atagacaaga tatctttacc ataaaaataa gttttagact ttgttactct gtattctaaa
    11101 tctttttcta aaccataata atttctaaaa tacagtacat cttcaataag tttttcatct
    11161 accccttgca cccttagatt atctattata tccatatttt tacccctttt atataaatag
    11221 tatttgtttc atttcttatc accattttag cataaaagga tataagctaa aattgatttt
    11281 ttaatctccc cattttaagt aaaaccatgc accattttat cactacaaaa ttaagagtac
    11341 tacttactga tatttcttga ttttgttcgt ctatacgtat ataatatatg ctgataaatt
    11401 atgaggaaag gatgattgaa atatggatgc ccagaagtag caaaaaaatg gggtatttct
    11461 gaaagaagag tacaaaaact atgtgaagaa agtcgcatac caggagtttc aaaactcggg
    11521 tatatgtggc tgataccaaa gaacgcagaa aagcctgttg atggtagaac aaaaagaaga
    11581 aaggaagtac aatatgaata gcgttttgat tatagatgat gacaaagaac tttgtgctct
    11641 tatgaaaaaa tgtgttgagc aagaaaattt atctgctatt actgcacatt gtggtgcaga
    11701 aggtctacat ttggtcgata aaaatagaca cacactctct cttgtaattt tggacataat
    11761 gatgccagat atagacggtt ttcaagttct gaaaaaaatt agagaaacaa gtaatattcc
    11821 agtaattatg cttactgcta aaaatgacga ggatgataaa gtttccggac tacgtttagg
    11881 tgctgacgat tacctaacaa aaccgttcag cataaacgaa cttatggctc gtgtcaattc
    11941 tcttattcgt cggtatacta tgttaaaccc tgcttttata acagacacag attgcataat
    12001 aataaatggc atgattattg acaagacaaa ccgaattgtt tctgtcaata atattcctgt
    12061 tcaacttaca agcaaagaat ttgatttact tgcttttctg gcttctaata agggacgggt
    12121 gtttaccaaa aaacagattt atacacaagt atgggaagaa gaatatgctt ttgacgatag
    12181 taacatcatg tctttcatca gtaaattgag aaaaaagatt gaaccagacc ccaaccatcc
    12241 attttatatt ttgacagttc gtggtgtcgg ttatcgtttt aataaggagg cttaacatga
    12301 atacaagtgt ctatctgctt ttgatattgg ttcttagcct gattattata gcgtaccttg
    12361 tttctgtcct gctacgtgtc cgaatacaac ttacacttat aaaagatgct ttagaggata
    12421 taaaaaatgg aaaccttaat cgacgtattt taacagatga aaacgacatt acaaaacaaa
    12481 tttgctatga tattaacgaa atagcaatca atagccaatt tcaacttatt cagcaaaaac
    12541 aatcagaaca agcctacaaa cagctaatga caagtctctc ccatgatgta aaaactccac
    12601 ttgcttcttt ggtcggttat ttggaagcag tagaaagtaa aattgttgta ggagaggaaa
    12661 aagacgagta tatccatgtt gcgtctaata aggctcatta cttaaagaat tttgtagaaa
    12721 atctatttga atgggtaaaa ttggattcta aggaacaagt ttttcatttt gatatttttg
    12781 acttgaatga attatcccgt aatattatat ctgattggat acctgtttta gaaagcagtc
    12841 attttgaata tgaatttgat ataccagaaa tagagtactt cttacgtata gacgcaaatg
    12901 catatacccg tatcattaat aacctattac aaaatattat tacccatagt agtggcaaca
    12961 aaatgacatt gagaattttt gaaaacaaag agcaagctca aattataata acagataatg
    13021 ggaaaggtat atcttcagac aatctcccac atatttttga aaggttgtac caatgcgacc
    13081 attcacgagc ttcaaaggga aatggtttag gcttggcgat tgcaaaggaa cttattaacg
    13141 ctcataaggg aaatattacc gccaacagca ctcccggtat gggaacagaa tttacaatat
    13201 tgcttcctaa agccctgtaa aagtacgagg ctttcttact ctaaaacaag ataaaagcaa
    13261 gattatggca aggttttggc aaggttcatg ttttataatt ctaattgtag ttgattttga
    13321 cgttgtatca actatctttt gtagaaacaa atttattaaa agtgaggaaa ttatatatga
    13381 atgattttgt gattgaaaca aagcaactta caaaaattta tgggaatcaa acagtagttg
    13441 acaaagtcaa tctccatgca aaaaaaggcc gaatatatgg tctgttagga cgcaacggag
    13501 ctgggaaaac cacaattatg aaaatgattt taggattgac ttcaatcacg tatggcgagg
    13561 tagatgtatt tggagagaat attaagggac gtgaaaaaca agtatatccc cgtatcggtg
    13621 caattatcga aacgccagga ttttatccta atctgacagg aacggagaac ttggagattt
    13681 ttgcaaaact gcgtggtaca gccgccccta attctgtaaa aagagcattg gaaattgtag
    13741 gcttacctta taaaaataaa aagctgttta gtgaatattc acttggcatg aaacagcgac
    13801 ttggtattgc caacgctatc ctgcatgacc cagaactgtt agttttagat gagccaacca
    13861 atggtcttga ccctattggt attgcggaga tgagaaagtt tattagggac ttgagcgttg
    13921 agcatggaaa gacaattctt atttccagtc atattctttc agaaatctca ttgttggctg
    13981 atgatattgg aattatcgac cacggtgttc ttttggaaga aagtagtatg agtgaactag
    14041 aaaagaaaaa cagtaaatat atcctgctac aagtttcaga tattccgaag acatcactta
    14101 ttttagagcg tcaatttaac acgaaggatt attccgttca agatgactac acgttacacc
    14161 tttataacac taatctggat atgggtgaaa tcaataaagc acttgtaatg cagaatgtaa
    14221 ctgttattag ttctcaactt tgtaatgata ctttagagga ctactttaaa aaaatcacag
    14281 gaggagaggg cattgcttaa acttattcaa acagaatttc ttaaactgcg tcgtaggaaa
    14341 cttatttggc tgatgttgtt gtctgctctc attatgccgt ttttcgcact actatacttc
    14401 aattattttg gtaaaacagg tgttgagcca ataaagtttt ataaatggtc tgcatttggt
    14461 tatacacttt ggattatttt gcctgttgtt ttgggtatat tttgcaccat gcttatacac
    14521 gaagaaaacc agtatgatat gctcaaacag ctttggattg tacctattag taaaatggga
    14581 tactttttta gtaaattttt cgttgttttg atttactcta tttgttttat gctgattaca
    14641 gccgttgctt ctgtgttatt tagtgtactt tctggatatg tcgtatttag ctgggcaagc
    14701 attttatatt tgttgaaaaa atgtttagaa attggtgtaa tcactgcttt tgtaatgttg
    14761 ccgattttgg caatcgctac atctcaaaaa ggatacattc tcccaatttg tatcacattg
    14821 gtatatgcct tttccggttt tgttataatg acaataaata tgtatttaca cccattatcc
    14881 agtatgggag ttattatcat gagaaataaa gacattccag gtcttatatt ttcacaggaa
    14941 attaatgtcc cattagcatt tctttgtatt tgtatttgga gtattgtatc agtattgttt
    15001 gcaaacactg cactaggaag aagaaagtga ggtgtagata atgtggaaat tactttgggc
    15061 tgaatgtcaa aaaattcgtc gttcaaaaat tgtttggatt acagtttttg catccattat
    15121 gatagcagtc atagtttttg caggagggca ggatgtatat aatgacccag atttacatta
    15181 tggcttaaag agcgtttatg acggttcacg gtatatagac aatgcaggtt ggtatatgga
    15241 cgcagtacaa ccttggtcaa ctttttttgt tcttcctgct gtcattgccc tattaggaag
    15301 ctatattatt tgtcgtgaag aagaggagga cactattaaa tccctgcgtc ttattcccat
    15361 aaatgaaaca aaattaacca tagcaaaaat gattattact tttatattca gtatacttct
    15421 ttatttgtta ctctttacta taacattttt gactgaatcc gttttacatt tttccgattt
    15481 atcgacaaaa ctggttttaa gttgcatgaa agagtatttt cttgatggca taggtgtatt
    15541 ctttgcaatt tctcccatta ttgccttggt atcgcgcatg aaaaaaggat attggcttgc
    15601 attagtattc acagaagttt attctattgc tggactattt gcgggtatgt caaatgtctt
    15661 acaaaccttt tttcctattg cggcaatatt taatatttct ggatactata taactactgg
    15721 agaaaaaatt atggggagta tcataagttt attactttgt gcctgccttt ctgcatttat
    15781 acttaaaggg ctaaaacaca atgaaaaaaa ttgaaatagg tatatcaaaa tgaaaaagat
    15841 aaaaaaactg attattctaa ttgtattctt gtttcttttt ggcatgttac actgttatac
    15901 tgaatggaaa ttcaacaata acccacctaa aggggtaata gaaagaataa atgaatggac
    15961 tgataagatt ggactggatt agtaacttat gttaattata gtgaatgtaa tattagcaag
    16021 ttattgtttc gtattttaat actataactt taaatatttg gttttaatct tccaaacgaa
    16081 tataaaaaaa ctgtcacaga ggcaacatat ttttgaatat ttataaagtg gtagttttga
    16141 ttttcaaagc tgccgccttt tcttctatat aagcaaactt atcctcaaca aagtgtaaaa
    16201 catggtacat tttaagaaaa aagtaccaca gacacaagca ataaaagaaa cgccgagaac
    16261 ctacaaaaac gtagtgctta tgcagattta cgacgccttc tattttctca accgtcttaa
    16321 tgaccttgta caaaagaaat ttttctaata ttttttataa tcttatcttg tgatgttttt
    16381 tatgtataaa tgacttaata acaaatccaa taaaaattat ggatattggt ataattagaa
    16441 cgaggaattt ataatcatta taatgtgaca ttattcttac ccaactctca gaaaaaatat
    16501 atcctatact gattaatgaa gtattccata tggttattcc cacgatagat gatattacaa
    16561 aagttataat attttgacct gctatacctg ctataaaaga tatgtatgtt ctacataaag
    16621 ggattaatct acatacacaa acagaaatag aagagtattt gttaaaataa tcttgagatg
    16681 caaatattcc tttctttgcc tttggacata tctctattat tttatttatg atagatttac
    16741 ctccccaatt tcccactgta taacaaaata tcgaaccaag tatacctgca attatactta
    16801 aaattaaaat tgtcagaaat ccaaaatgat ttcttgcagc aattgctcca cataaaggta
    16861 aaacaacttc gctaggtaat ggaaaacaag catactcaat cataattaat acaaatacag
    16921 agattaatcc atattgtaaa ataaaactat tgattacaga cacttactta tctatctcct
    16981 ttcttattta aaggttttaa gggtacatgt tctaaggatt ctcaccctat atttaaagaa
    17041 tcaatctctt taaactatta tatgtattta cacaaagcat ttataacata ctttaaaaat
    17101 agtaccttaa aataagaaac ttaataaata aaaaaagatt acgtggctac gtcctactct
    17161 cccaggcag
//
LOCUS       DAMJZE010000407        12105 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.guided.120, whole genome shotgun sequence.
ACCESSION   DAMJZE010000407 DAMJZE010000000
VERSION     DAMJZE010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.guided.120"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            158..1564
                     /locus_tag="PC541_003961"
     CDS             158..1564
                     /locus_tag="PC541_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2841319.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1592..2440
                     /locus_tag="PC541_003962"
     CDS             1592..2440
                     /locus_tag="PC541_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2841320.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3101..3793
                     /locus_tag="PC541_003963"
     CDS             3101..3793
                     /locus_tag="PC541_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2841321.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3790..4725
                     /locus_tag="PC541_003964"
     CDS             3790..4725
                     /locus_tag="PC541_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2841322.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            4873..5796
                     /locus_tag="PC541_003965"
     CDS             4873..5796
                     /locus_tag="PC541_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2841323.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5780..6550
                     /locus_tag="PC541_003966"
     CDS             5780..6550
                     /locus_tag="PC541_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2841324.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6560..7342
                     /locus_tag="PC541_003967"
     CDS             6560..7342
                     /locus_tag="PC541_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2841325.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7719..8255
                     /locus_tag="PC541_003968"
     CDS             7719..8255
                     /locus_tag="PC541_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2841326.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8287..8676
                     /locus_tag="PC541_003969"
     CDS             8287..8676
                     /locus_tag="PC541_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2841327.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8713..9099
                     /locus_tag="PC541_003970"
     CDS             8713..9099
                     /locus_tag="PC541_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2841328.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9226..10668
                     /locus_tag="PC541_003971"
     CDS             9226..10668
                     /locus_tag="PC541_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2841329.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10965..11780
                     /locus_tag="PC541_003972"
     CDS             10965..11780
                     /locus_tag="PC541_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2841330.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4362 a         1495 c         2280 g         3968 t
ORIGIN      
        1 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
       61 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
      121 acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
      181 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
      241 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
      301 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
      361 agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg
      421 agagataaac cagaaaggtt tagaatttta taataagata atagacaaac tgatagagtg
      481 tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa
      541 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
      601 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
      661 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
      721 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
      781 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
      841 tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta
      901 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
      961 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
     1021 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
     1081 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
     1141 taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc
     1201 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
     1261 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
     1321 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
     1381 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
     1441 taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag
     1501 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
     1561 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
     1621 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
     1681 ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct
     1741 tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag atgcagaata
     1801 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
     1861 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
     1921 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
     1981 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
     2041 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
     2101 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
     2161 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
     2221 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
     2281 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
     2341 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
     2401 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
     2461 atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg
     2521 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
     2581 ttatatcatt acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta
     2641 catttctcaa catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga
     2701 gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt
     2761 tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct
     2821 tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata
     2881 cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca
     2941 ttacacttga agaagtaagt gataaatgag gtataaatcc tcatatgata aactactatt
     3001 tcattgagga acatattcta agtgatgtca agatactaca atatgactaa attcctagtg
     3061 ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat
     3121 agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga
     3181 agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca
     3241 gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat
     3301 acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa
     3361 agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga
     3421 atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga
     3481 ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac
     3541 taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc
     3601 aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc
     3661 atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga
     3721 actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa
     3781 cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt
     3841 ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta
     3901 aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta
     3961 attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat
     4021 tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca
     4081 agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta
     4141 cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga
     4201 gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat
     4261 gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa
     4321 gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat
     4381 cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa
     4441 aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga
     4501 atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat
     4561 atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt
     4621 ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag
     4681 attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc
     4741 atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa
     4801 ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg
     4861 cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt
     4921 gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg
     4981 ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa
     5041 ccaactactg gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt
     5101 cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca
     5161 gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca
     5221 gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt
     5281 ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt
     5341 ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag aacgtttatt
     5401 cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat tctttctgaa
     5461 attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa
     5521 agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca
     5581 gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat
     5641 aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc
     5701 attctaaaca acattgaggt atcagaatct catttatgcg aagatactct tgaagattac
     5761 ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa
     5821 actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc
     5881 cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca aattagttgt
     5941 caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt
     6001 ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg
     6061 aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc
     6121 agggttagga gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt
     6181 aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt
     6241 ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt cttttgtata
     6301 tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat
     6361 attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc
     6421 cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg
     6481 tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca
     6541 ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag
     6601 atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt
     6661 tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt
     6721 tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct
     6781 tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt
     6841 acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct
     6901 tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca atatatctgt
     6961 tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt gttatgttgc
     7021 tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg
     7081 gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt
     7141 ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta
     7201 tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact
     7261 tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca
     7321 taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt
     7381 gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa gctacagttt
     7441 tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt
     7501 gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt
     7561 atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa
     7621 aataatatta tatatagttg acaaaaattg gcaaatgaga taatatataa atatagaaat
     7681 agtaatcatt actattatta aaaagggagg aattaattat gaacttaaaa ggaactaaaa
     7741 cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat
     7801 actacgcttc aaaagcaaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa
     7861 ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc
     7921 cttcaacaga ggaaaattta aaggatgcag cagcaggtga aaactatgaa tggactgata
     7981 tgtatgcaaa atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat
     8041 ttgaagctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa
     8101 acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact
     8161 gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg cccagtatgt gaccatccaa
     8221 aagcgtattt caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg
     8281 ttaactatga aattttctaa acaacgagaa ctgattttaa atgaaatatt aaataatccg
     8341 gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatttaagc
     8401 ttaggaactg tgtatagaaa tttagctcag ctaacggaac atggctttat aagaaaggtt
     8461 agtattccag ggtatccaga tagatttgat ggtcgaatag ataatcacta tcatatcata
     8521 tgtgaagtat gtggagaggt atacgattta gaatccgaag ttcttaataa tttacaagaa
     8581 ttaatatctg aggagacaga cataaaaata acatcttata acataagttt taaagggatt
     8641 tgtaataatt gtaaaaggtg tagccaagta ggttagtttt caaattctaa aaataattaa
     8701 tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg tggaaacctt
     8761 gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggaa
     8821 cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtaat tgaagtggat
     8881 ggaaataatg ttactgttaa agtaagtagc acaactcatc caatgactaa agaacatcat
     8941 atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat
     9001 ggagaacctg tagttaaatt tgctttgaat gatgatgata aagtgatttc tgcctatgct
     9061 tactgtaatt tacatggatt atggaaggct gaactgtaat atttaaataa attgagtcaa
     9121 aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcaa
     9181 gttttaattt tgtttaatgt caatataata taaggagaaa ataatatgtc aatttataaa
     9241 tgtagtgttt gtggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta
     9301 agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt
     9361 actgtttact atgaagaaga tgaaaagata gcagaagaca tagttgaaga tgaatctaaa
     9421 ttaaatactg aaaaagaggg agatttaaat tacttaagta catatttaag aagagatgat
     9481 gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata
     9541 gaacctatga gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct
     9601 caattaaatc cattaccttt aaatgagcat gatgaagtaa atacaacaac aataatagga
     9661 aaaaaagcaa agaaacctat gattatagaa aatccagttt atatttccca catgtcattt
     9721 ggagctttgt ctaaagaact aaaaatagct ttagcaaagg gtgcagccca aaataagaca
     9781 gctatgtgta gtggtgaagg aggtatatta ccagaagaga aggaagcttc atacaagtat
     9841 atttttgagt atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat
     9901 gctatagaga taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggc
     9961 gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc ctgtaggtca agatgtaata
    10021 agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa
    10081 cttagagaag tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg tcatattgaa
    10141 aaagatatgg aatttatagc ttatgctaaa cctgattttg taactattga tggtcgtggt
    10201 ggagctactg gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc aacaatattt
    10261 gctctttatc gtgctagaaa atacatagat acacatggat tagatataga tttggttata
    10321 acaggtgggc ttcgtatatc tactgatttt gctaaggcaa ttgctatggg tgctgatgca
    10381 gttgctattg caagttctgc tcttatggca gcggcgtgtc aacaatatcg tatatgtggt
    10441 tctggtaagt gtcctgtagg agtagctact caggatgagg aattgagaaa aagattacat
    10501 atagaaaaca gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag
    10561 acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat
    10621 actgttaact ctgaaatatc aaattataca aacatacagc atgtatagat tgaagtttga
    10681 aactattaga ttttagatag ctataaaagt aaatgataaa tatattttat aaaagagagt
    10741 gtcttaaaat gaacttttta gttcataagt cactcttttt tttatgaagc caaatatatt
    10801 tatcatttta gcaatgaaaa agaatcttat ctctttattt tgagatagac tccttctatt
    10861 tatgaaaatt aaagatatta aattgtaaaa tattctgtga tgtcgtacta ttaaactaaa
    10921 gattctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa agattgaaga
    10981 tacaagtttt agttgacaat aatacttata ttgatagata ctttgttgga gaaccagcag
    11041 taagctatta tattgagatt gatggaaata ggattttgtt tgactcaggg tattcagatg
    11101 tattcatatc aaacgctgaa aaattaaata tagatttagg aaatttaact catgttgttt
    11161 tttctcatgg gcataatgac catacaagag gtatccagtt tctagaaaat aggtatgatt
    11221 tatctacagt tgaacttatt tcacatccta actgttttat acctagaaaa catggagaga
    11281 aaagcatagg agctccattt tctgctgagg aaattaaaaa tatttttatg tataatccta
    11341 aggataagcc atttaatcta agtaagaatt gtgtgttttt aggtgaaata ccttctataa
    11401 atgattttga aaaaagagct aaaattggta aatgtaaaat aggagatttg tgggaagatg
    11461 attatgtttt agatgattct gctatagttt gtaaaactga taaaggtatt tttattgtta
    11521 caggatgttc tcatagtgga atttgtaata tagtagagta tgctaaaaag gtatgtggag
    11581 atgatagagt tattggtata ctaggtggtt ttcatctttt tgaattgaat aataggttag
    11641 atagtacaat acaatatttg gataaagaag aaatgagaat gttgtaccct tgtcactgtg
    11701 tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa gttggagtag
    11761 ggctgaccat aagtatatag tatttgaaag tatataagat ttaagttgaa ggttaaaaag
    11821 atattttcta tagaaaatat cttttttgtt acgtttattt taagagaaat caatatactt
    11881 ttatatgaaa tattgtaaaa aaatttattt ttaatgagaa tatattttca ttaataaaca
    11941 attttattat gtattaataa tgttagaata aatataaata aatcaaagtt acttacatta
    12001 cagtatattt tttcacaaaa tatattgatt tattctttta aagtttgata attccaatga
    12061 atgtaataaa agaaggaatt ttatactata cgattattat tatat
//
LOCUS       DAMJZE010000408        11096 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.guided.128, whole genome shotgun sequence.
ACCESSION   DAMJZE010000408 DAMJZE010000000
VERSION     DAMJZE010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.guided.128"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            232..570
                     /locus_tag="PC541_003973"
     CDS             232..570
                     /locus_tag="PC541_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841331.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            592..909
                     /locus_tag="PC541_003974"
     CDS             592..909
                     /locus_tag="PC541_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2841332.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1059..1736
                     /locus_tag="PC541_003975"
     CDS             1059..1736
                     /locus_tag="PC541_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841333.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2064..2831)
                     /locus_tag="PC541_003976"
     CDS             complement(2064..2831)
                     /locus_tag="PC541_003976"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2841334.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3503..4318)
                     /locus_tag="PC541_003977"
                     /pseudo
     CDS             complement(3503..4318)
                     /locus_tag="PC541_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4419..5117
                     /locus_tag="PC541_003978"
     CDS             4419..5117
                     /locus_tag="PC541_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2841335.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            5205..5825
                     /locus_tag="PC541_003979"
     CDS             5205..5825
                     /locus_tag="PC541_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2841336.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            5981..6763
                     /locus_tag="PC541_003980"
     CDS             5981..6763
                     /locus_tag="PC541_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2841337.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            6888..7814
                     /locus_tag="PC541_003981"
     CDS             6888..7814
                     /locus_tag="PC541_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2841338.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            7828..8277
                     /locus_tag="PC541_003982"
                     /pseudo
     CDS             7828..8277
                     /locus_tag="PC541_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8637..9044
                     /locus_tag="PC541_003983"
     CDS             8637..9044
                     /locus_tag="PC541_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2841339.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            9046..9276
                     /locus_tag="PC541_003984"
     CDS             9046..9276
                     /locus_tag="PC541_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2841340.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9666..9869
                     /locus_tag="PC541_003985"
     CDS             9666..9869
                     /locus_tag="PC541_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2841341.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            9947..10300
                     /locus_tag="PC541_003986"
     CDS             9947..10300
                     /locus_tag="PC541_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2841342.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10372..11004
                     /locus_tag="PC541_003987"
     CDS             10372..11004
                     /locus_tag="PC541_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2841343.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4084 a         1570 c         1745 g         3697 t
ORIGIN      
        1 ttattaattc gttttattat tgtagtatgg ctatgaaatt atgaaaatat ttctctaatt
       61 aaaaatatga tttatccata tttgtttatt gtagatacag actatacaaa aataaatagc
      121 acttatttca gtataaaaac atatatagtc tatatttaga ctaaaaaagt ataaatagtc
      181 tatataaaac aacttaaact atgaatataa tctaaagtgg agggataata attggcaaat
      241 ataggaaaaa taataggaga tagaataaaa gtctatagaa caaaattggg atatagtcaa
      301 gagtttctag cagaaaaggc aggtttacat ccaacatata ttggtcagat tgaaagagct
      361 gagacaaata catctatcaa tattataatg aaaattgcta ctgctttaga cataccactt
      421 gaattgttat ttgcaaatat tattacaaca gaaggcgttg ataactctgt acctttagaa
      481 tgttatgaaa tgattaataa attaactgaa aaagaacaaa ttgcaatgct agagttaata
      541 aagtgcatca ttaaatacaa agaaatataa gtgagggaaa tagtaacaat aatgaattat
      601 ataggtaaaa gacttaaaga agaacggaaa aaagcaaatt taacttcaaa agaatttgca
      661 aatatggttg gagtatcgcc ttggtatata actcaaatcg aaagtggtaa aaagaatcca
      721 agtcttaaaa cattcataaa atttgtaaat atattaaata tttctgcaga tgttcttata
      781 aaagatatta cttccactgg aaaaacatat ttagaaaatg atataaatga agagttaaaa
      841 gacttaaatt caagagaact aaatctgata tcacagatta tagacatcat aaaaaagaat
      901 aaaatataat tctttatatt ttatataaaa aaaaaggtta tttgaaataa ataacctttt
      961 tttgtctttt tgtgttaaca ttgtcttata gtgctaatga gtactattag accatgttct
     1021 atggccttat tttaatataa aacgattgga gataggctat gattgtgatt gaaggtagcg
     1081 ataaatttaa gattgcaaaa gaatatattg atgtagaata tactcttttt agcaaagtaa
     1141 cttttaggta tgaaaagttg aaatttaaag ataatgctga attggaaaaa attaagatgt
     1201 ttaaatataa aaatggctac atccctaata aattaaacct ttcttttgga tatggattct
     1261 cttcttataa aaagcaaata attagagaaa ctgtagatac tttaagattg acagaaattt
     1321 tttcaagcga gaacatagaa gatattaaat ttataaaaga tggtacaaaa aaattagaaa
     1381 ttagcataga gaaagttgtg aaatttaaac gtcgaaaaaa aagaaattat gtttgttgct
     1441 attgccctga tatgtataga gacataaaac tcgacaaaga atctatcaat aagatataca
     1501 acagaaaaat aaaaatagaa agagaagtta atatttttga agatgaggat gttataataa
     1561 acaagaaagt attgaagttt ccaaagtctt ggacaaagaa tatgcaaaaa tattggttaa
     1621 gtgaaaataa gtatcccata cattctactg taattgatga tgatagatat aaatgttgta
     1681 atgtacaata tacaaaaaat agagtgataa tattatatta catatataac cattaatata
     1741 cataattaca aaaaaataat gacaaatata cataatacaa atatatcttc tactttagat
     1801 aaaacagtta ttatgaaaac ttactatcta taaataatag aaatgctagt ataccatcta
     1861 taatctaatg tctagtaggt tttaatggca tattttaaat atgccatagt tgatttataa
     1921 gattttctat atcactttca aaatacacac taaatagttg cttttcagat ataattgccc
     1981 aatttaaatt ttactatctg aaaagttttt tatatttaat ttatatcttc aaaaataccg
     2041 tctaatattt ctatataatt aatttaatta atactcttat gtaatagatt aagtactgaa
     2101 tttaactctt taaatgatat ttgccctggt gctgaaacac tttttgcagc tccaaaagtc
     2161 aatgcagaac caaatatttc accacacaat ctacttataa cacccattcc tgacattgac
     2221 attgttatta ttggtctatc tgcataaatt ttaaacattt catttgtagc ttcaagtagc
     2281 actaacacat ctttttcatt ttgcggcata acagctattt tgggtaaatc tgcgcccaat
     2341 tcctgcattc tacataaacg agatacaatc tcttcttttt ttggagtttt attaaaatca
     2401 tgattagata taattacttt cacttctttt ttatgagcaa aattaactac ttcatctatg
     2461 acctcatctc ccataaataa ctctacatct attaaatcta ctaaacctgt attagaaatt
     2521 tctttattca aagtagtata ataatctctt gatattaatt tttctccccc ttctactaca
     2581 cttctaaatg tgaataataa agggatatca tgtatataac ttcttaattc atagagaact
     2641 tccttaactt ctttaatatt ttctacattc tcaaaaaaat caactcgcca ctctataata
     2701 tccaaacatg catcttttaa ctctttagcc tcttttataa tatctttttt atttttccca
     2761 ataattggta cacaaatttt aggtctacct tctccaatag taatattttt tacttgaact
     2821 tttcttttca tattctcctc cataatcaga aattctatag gactttaaat tctattttct
     2881 gcacaattta attatacatt aaaaatacta tacattataa aaatactaat ttaaaaataa
     2941 tttttattct aattcttaaa acaatcccac atattttaga aaaggtactc tataatatac
     3001 aaacacttct tttttaccta aatttatttt aacatcctat aattttcaca atattcttta
     3061 aattttactc atataaacaa tattattctg gatgctaata gaattaatct ataaatcata
     3121 gtataattat gcagaaataa ctcgaatttc actacttatg aagagattga atattaatga
     3181 atctaaatca tttgcattac ttaagagttt tgactaaagt tgaacaccat acaaaagtag
     3241 ctactcaatt gtttataccc caaccaagct taagtaatgt tatatagatt ctcgaaaaag
     3301 aatttggtat ctttttattt gaaaaactta tattaaattt gattatcttt tcataagttg
     3361 gtaaatgtcg atatatcctg tatctacaag gagtatcggc attttctttt tggaagatac
     3421 tttgcataag ttggcattta accgtataag aatgccatca aaagtagtaa ataagtagta
     3481 aactctagtc atactactag gattataacc gttccatttc agctctcgaa gaactaaatg
     3541 aaccatgtgc ataatagcca agcgtcatag taatgttttt atgccccata atatattgca
     3601 atatattagg actcattcct ttgttagcca tatttgtaca gagtgtatgc ctaaaggtat
     3661 gtggcgtaat ctttggtagc ttgtcctcat gggtcttatt atacttcttg actaacgctc
     3721 ttaacatgct ttgataatga gttgcttgtt ttggtaatcc atcttgttta agaaatagga
     3781 agttctggta accatcaaca atgattggtt ctgctttagc gttcttacgc cgttccacga
     3841 tacgttcaaa tgattgataa gctctttcag tcattggaat ttctcgctta ccctgtgtgg
     3901 tctttggaac ttctacataa tagccaatct tagtatcact caacaattga tggtctacca
     3961 taatggcttt atttttcata tccaattcta aagtcaaacc gcataattca gaaatgcgta
     4021 gtcctgtttc taagaggata acaacatcat cataatattt acaataagtc ttatcgctcc
     4081 ccataaagga taataagccc atttcctgtt ctggtgttag agcgattttt ggttctgttg
     4141 tatcttccaa aacatcactt aactaaaaat taaatggatt ctttctaata cagtcgtctt
     4201 gaattgccat gaaaaacgac gctttcagtg agcgcttata attgtcaatg gatttataag
     4261 cacacccatc ttcactcatt cggatagccc agcttttagc gtctgataat ttaaccatat
     4321 caatgctttt tgcacctagt gggtcattga tagtatctat attttgattt ataatttata
     4381 cattttttta aaaacagact tttaactgta aagaatctat gtataaactt agctatgttg
     4441 tccgtgtatc tacaaagaat cttgacgagc taaaaaggcg ttgtgatgaa gtcaaagact
     4501 tttacgacga cttatcaatc aagctcgtgc gtccctttgg ggatatgttg ggattgcatg
     4561 gcgaatttat tcctgtcagc aaacgttaca tcaatgatta tattcaatat gtcacttcgg
     4621 attttttggc tggacttggt tttggagcga cacaaaccct tggcgaacat gaggatatct
     4681 atcttggcta caatctggac actggaaaga atgtctatct aaaacctgcc cttgctagtc
     4741 aaggggtaaa gggttctatt accaatgcgt tagcgtctgc gtttatcggt tcattgggtg
     4801 gtggtaaatt attcagtaac aatatgcttg tttactatgc ggtactattc ggcggacagg
     4861 cggtcatagt tgaccctaaa gcagaacgcg gcaaatggaa agaaacgcta ccagaaatag
     4921 cccatgagat aaacattgtc aatttaacca gtaatgatga aaacaaagga ttgcttgacc
     4981 cttacgtcat tttgagccgt aaaaaggatt ctgaaagttt agcgattgat atcctaacat
     5041 tccttactgg tatctccagt cgtgacagtg ataagttttt gcagaaaaat gacaactgga
     5101 aaataataat ggaatagttt ctatgtaaat atatttttac tatcttacct aactaattga
     5161 aagatgatat aatttaatca aagatttggt aaggagggat gaatatgcat ttaattgatt
     5221 ttatgaatga agatattaaa aagaagcttg ttaaaaaaac gtataaaaag aaagagacta
     5281 ttctttttgc tgagcaagaa aatgaatatg taattcttat gattgacggt attgcagaag
     5341 cattcatctt aaatgtgaaa ggaaacatct caacgattca tctttataga agtggtagtt
     5401 tttttggaga aatggaacaa tttaatgatg ggaaaaaacc cgttgaaatt gtcgctttca
     5461 cagattgtaa tgtatataaa cttcatagag atgatttttt aaactggtta aggggagatt
     5521 ttgaagctac aaaatttctg attcgagaaa tatctagtaa attagttgta aatgctgaat
     5581 taattgaaga attatcatct ttaagtgtta aagaacgact tctgaggtgt atatccttac
     5641 actattatag aagtgaatta aatctgctta ccaaaaaaca gcttgctagc gaagtaaacg
     5701 ctccaattag aagcattaat cgtgctataa atgaatgtgt acaagaacat ctaattgctt
     5761 atgaaaacaa aagatttagt gtaattaatc agcgagaggt attaaagaat cttcctacaa
     5821 cataagtata atttcaaaaa agtattgaat aataattatt caatgctttt ttatttcggg
     5881 tcaagtgacc tatttttttt tatttcttca ttatataata ctcctatgca taattatgtt
     5941 ggattgatag ctaataaaag acagtgagag gtgttatatt atgactaaaa cacaacagaa
     6001 aatgatacaa aacgtattag aattttcaaa aagagatagt aggataatag gacttgcaat
     6061 tagcagttct tatatcacaa aatcgttaga tgaatattct gatttggatt ttctgattgt
     6121 agcagatgat aaatcctatg aagaagttat cgaagagaga ttacttattg tggaacagtt
     6181 tggaacatta gtttctgctt ttactggcga acacgttgga gaacctcgat tgataatttc
     6241 cctctatgat tcccctgttc ttcatgttga ttttaagttt acaaccttag atggtttact
     6301 agatagggtt gaagattcag ctattctcta tgaagaaaac aactgcataa cagaaattta
     6361 ttctaaaaaa gaagcatact tcccaactcc agacttacag tggattgaag atagattttg
     6421 gatttgggtt cattacgctt gcactaagat tggacgtaga gaactatttg aaacaattga
     6481 ttttttatct tttttaagac aatcagtact tgccccatta atacagcttc tcttaggtaa
     6541 attacctcgt ggagttagaa aaattgaatt ggacgcaccc caatatattg aagcattaga
     6601 aaagacggtt gctaaccatg aatataaaag ctgtgttaat gcattgcagg ctactatcaa
     6661 cctgtatctt gagttaagag aggaattatc acaaagtcct attgtccgaa aggttgaagc
     6721 tgaaagggtt gcattacagt attttcaaga tttaatcaaa taaacccaaa gtaaaaaaat
     6781 attgaatagt aaatattcag tgttttttca tttttggtca aatgacctag attagttgtt
     6841 gtaacaattc tataattatt ttgaaagtaa cagtatggag gataactatg agtgatattt
     6901 taagtagaat tacaaataaa attggggata agaatatagt tgataagtta tcagctctat
     6961 caaaatcaga tttgaactca cttctattag aagtgtttga tagacaagca aatactttaa
     7021 ctgcaactga tattctaaag tcataccaac taaatagatt tactatccct agcggtatag
     7081 atccagaaga aattcatgct ttggaatcaa aattattgag aaaagcattc aatatggata
     7141 tcaagacgat tatgctttct ccctctgccc cattaggtag ctgtagcgtt tttggctccg
     7201 tagatcaata taacgtagta agtgctctac gagggacaga aacacttgca gacccctcaa
     7261 atatgttggc aataattatt gctgataaat taaaaagaaa agaagaaaat aataccatac
     7321 ctatccacat ggctactact gctagggtaa taagagcaca aaattttgta ggtaaaggtt
     7381 tatattcaca ttttggctta tattgtatgg tttcatctgg tatagataca gggtcttaca
     7441 cttgtgaaaa aatgttgtta gaaaagcact taaactatta taaagatata ttaagtgaaa
     7501 tagaaaatgt tcgtttctct attattctac gcaaaagaaa cggatacaaa gataatgacg
     7561 gatttttcga cagaatggtt gaaactatca aacttatttt tcctaatata gagttatcaa
     7621 ttcataatga tgatgttgat aataattatt ataaaggtat caatttcaaa ttatcggtca
     7681 aacaaggaaa tgaaaccgtt gaaatagttg acggtggatt tgttgactgg acttatcaaa
     7741 tgcttggaaa taaaagagaa cgctgtttga taagtggtat tggcttagaa agatttcttg
     7801 tagctgtttc ataaaaggag aagaaaaatg gaacttatag agtggaatta tgcttatatt
     7861 accgatttag ttgagtttgc gaatgataaa gaaattgcaa agaatcttcg tgacaacttc
     7921 ccccaccctt atagggagca aaacgctaga gattttgtta gtttttgttt ggcaacttca
     7981 gatgttaaac aacttagcta tgctattttt tatcaagaaa gggctattgg aagtatcagc
     8041 ctaacatttg aagaagatac ctctattaat aaaaatgctg aattaggtta ctggcttgct
     8101 aggaactatt gaggcaaagg gatagcaaca aatgcagtaa aagaaatatg caaaattgcc
     8161 tttgaaaaat acaaccttaa caaaattcat gctaatgtat attcctacaa tagagagtca
     8221 agtaaagtat tggaaaaatg tggctttaag attgaagaat actgtcacaa ttcataataa
     8281 agaatagaaa catgattgat tgccatgtat atagtttaat tcgaccgcaa tagcatttat
     8341 tagaattaac ctacttttta ataacagaat aagtttttcc cctctattaa tttcttatgc
     8401 atctttgtat ttttatgaaa agctagccaa tgcatatttc catacatcaa tatggtggtg
     8461 gcttacaacc aatataaatt aaatacgttt taaagaatac acatttttgc cctataggca
     8521 gagtgtgtat ttttttctct tttttttgaa atttatcgaa gatttaacgc cctcccaccg
     8581 tgataggagt aagcaaggtt atttaactcc ttgcgatgga aagagggtga aatcttatgg
     8641 ttgatgaaaa gacaattgaa catcaattcg acagtttctg taaaacagtt ttgcgtaact
     8701 atgctagaga tatttatgac gaaaataaac gcagaaatga ttatttggta tctctcgaat
     8761 ctttgtcact tgcagaatta agtaaattga gtattttaga tgattatgat tctaactaca
     8821 tctgtatggt ttcttacgac tacaacatac gaattgaaga tgtattaatg gcgcaagcaa
     8881 tcggaaaact tacaaaaaga aaacaagata taattctact ttcattcttt ttcaacatga
     8941 caaatgctga catagcaaca ttgatggatt tagcagaaaa taccatacat tatcacaaaa
     9001 caaatgcact aaaagagttg aaagaactca tgcaggaaca ttgaaatgaa acctactaaa
     9061 gaagtattac cttttacaat tattttactt gctgtacatg gcgatgtaaa tgcaataaac
     9121 gtaatactta aacattttga acactatatc attcgtcttt cacagaaaac tttatttgat
     9181 gaatacggaa atccctatat tcatgtagaa gaagaaacaa agcgaatgct aggaacaaaa
     9241 ctaatcactg ctattttgag atttaatttg tcttaatctc gccctctctc catgtatcaa
     9301 gctatttggt acatggagaa tcactaatta tttgtctata aaagcccaca agctattgtg
     9361 attttttata ggcaaatcaa tgccgactgt tctttgacaa ctgaataaag tagatagata
     9421 cgtttgtgat acagatagcc aacaaatact atacgccatg accttaatca agagagctac
     9481 tatatagtga tttgtgaata acctttggaa cggttgtttt gccgtaacct gtaccacagt
     9541 ataatgatac acctgtatga cacggttctg tccataagaa tgggattggt tgaaatacca
     9601 atggagctta tcaaagccat ctatctactt ctgaactacg aacatagaaa ggagtgatac
     9661 attatatgaa caatactgat gtacctattt gggaaaaata taccttgacg attgaagaag
     9721 cgtcaaaata ctttcgtatt ggagagaaaa aattacgtaa acgtgctgat gaaaaccttg
     9781 attctggctg ggtcattatg aatagcaatc gcattcaaat aaagagaaaa caatttgaga
     9841 aaatcgttga tacacttgac gttatctaat gaaattgagc cttatatgtt ataatattca
     9901 tatgacgtat caagctcaat tcggttattg agaggagctt cttatcatgt caactgaaaa
     9961 aagacgagat aataaagata gaattttaag aactggagag agccagagaa aagatggaag
    10021 atacgcatat aagtatgtat attcatttgg taaaccacaa tttgtttact cttggaaact
    10081 tgtacctact gataaacccc caaagggaaa acgtgatgat atatcattaa gggataagga
    10141 aaaagcaata caaaatgacc ttgatgatag cattgacact attggtaaga aaatgacagt
    10201 ttgtcagtta tacgctaaaa agaatggact aagaaagaat atccgactta acactaaaaa
    10261 aggacgccat tatcttatga agattcttaa caaggagtga ttctgtgaag agatgtattg
    10321 tagtagtgga atagtcaaaa acataatata tattaaagga gagatttaaa tatgactatt
    10381 ccaagttcaa ataaggtata tccaagaagt aatgattatc aaactgtata tcttaaaaat
    10441 gtaataacaa gggaaaatat agaagttgga gattacacaa tatataatga cttttataat
    10501 gacccaagag attttgagaa aaacaatgta ttgtatcatt accctataaa caatgataaa
    10561 ttaataattg gtaagttttg ctcaatagca tgtaatgcaa aatttctaat gactagtgga
    10621 aatcactcaa tgaaatcaaa atccacatat acttttccca tcttttatga agaatggaaa
    10681 ctcgatatca accatataac ggacgcttgg gacaataagg gtaacattat aattggaaat
    10741 gatgtgtgga taggttatga ttctataatt atgtcaggtg taaatattgg tgatggtgca
    10801 attattggaa caagagcagt agttactaat gacgttccac cgtattctat tgttggagga
    10861 gtacctgcaa aaattataaa aaagagattt aataatgata caatttcaaa attattagaa
    10921 attaaatggt gggattggcc atataaaaaa attcaatcaa acattaagca tattcaatca
    10981 ggaaatattg ataaattagt ttaatatttt ataatttatt tatattaaac tttactttaa
    11041 taatcatttt ataagagctt tttcaaaatt tgtatattat tttaatatat ataaat
//
LOCUS       DAMJZE010000409        10292 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.guided.148, whole genome shotgun sequence.
ACCESSION   DAMJZE010000409 DAMJZE010000000
VERSION     DAMJZE010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.guided.148"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            246..863
                     /locus_tag="PC541_003988"
     CDS             246..863
                     /locus_tag="PC541_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2841344.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="PC541_003989"
     CDS             971..3502
                     /locus_tag="PC541_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2841345.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="PC541_003990"
     CDS             3936..4637
                     /locus_tag="PC541_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2841346.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5769
                     /locus_tag="PC541_003991"
     CDS             4687..5769
                     /locus_tag="PC541_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2841347.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="PC541_003992"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="PC541_003992"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2841348.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="PC541_003993"
     CDS             7127..7933
                     /locus_tag="PC541_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2841349.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="PC541_003994"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="PC541_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2841350.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         3911 a         1076 c         1852 g         3453 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt ataaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt atctttaagg ttattattcc gattgtagga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tataggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaga ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
    10261 atataataaa ataattatac cttaactgta aa
//
LOCUS       DAMJZE010000410         2483 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.guided.295, whole genome shotgun sequence.
ACCESSION   DAMJZE010000410 DAMJZE010000000
VERSION     DAMJZE010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.guided.295"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            62..538
                     /locus_tag="PC541_003995"
     CDS             62..538
                     /locus_tag="PC541_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2841351.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
                     QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
                     GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
     gene            1233..1292
                     /gene="ermCL"
                     /locus_tag="PC541_003996"
     CDS             1233..1292
                     /gene="ermCL"
                     /locus_tag="PC541_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDF2841352.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
     gene            1353..2087
                     /gene="erm(C)"
                     /locus_tag="PC541_003997"
     CDS             1353..2087
                     /gene="erm(C)"
                     /locus_tag="PC541_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="HDF2841353.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
                     PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
BASE COUNT          958 a          377 c          387 g          761 t
ORIGIN      
        1 tttttttttt agaacagtta tgatatagtt agaatagttt aaaataagga gtgagaaaaa
       61 gatgaaagaa agatatggaa cagtctataa aggctctcag aggctcatag acgaagaaag
      121 tggagaagtc atagaggtag acaagttata ccgtaaacaa acgtctggta acttcgtaaa
      181 ggcatatata gtgcaattaa taagtatgtt agatatgatt ggcggaaaaa aacttaaaat
      241 cgttaactat atcctagata atgtccactt aagtaacaat acaatgatag ctacaacaag
      301 agaaatagca aaagctacag gaacaagtct acaaacagta ataacaacac ttaaaatctt
      361 agaagaagga aatattataa aaagaaaaac tggagtatta atgttaaacc ctgaactact
      421 aatgagaggc gacgaccaaa aacaaaaata cctcttactc gaatttggga actttgagca
      481 agaggcaaat gaaaaacaag aaaatgcatt atctgattat tattctttca aggactagta
      541 taacataaaa tcgtctacaa atagacaaaa aacctgcacg cttaatgtag atcaaaagct
      601 taacgcaaat gaaatagatt gacctcccaa taacaccacg tagttattgg gaggtcaatc
      661 tatgaaatgc gattaagctt tttctaattc gcataagcgt gcaggtttaa agtacataaa
      721 aaatataatg aaaaaaagca tcattatact aacgttatac caacattata ctctcattat
      781 actaattgct tattccaatt tcctattggt tggaaccaac aggcgttagt gtgttgttga
      841 gttggtactt tcatgggatt aatcccatga aacccccaac caactcgcca aagctttggc
      901 taacacacac gccattccaa ccaatagttt tctcggcata aagccatgct ctgacgctta
      961 agtgcactaa tgcctttaaa aaacattaaa gtctaacaca ctagacttat ttaattcgta
     1021 attaagtcgt taaaccgtgt gctctacgac caaaactata aaacctttaa gaactttctt
     1081 ttttcttgta aaaaaagaaa ctagataaat ctctcatatc ttttattcaa taatcgcatc
     1141 agattgcagt ataaatttaa cgatcactca tcatgttcat atttatcaga gctcgtgcta
     1201 taattatact aattttataa ggaggaaaaa atatgggcat ttttagtatt tttgtaatca
     1261 gcacagttca ttatcaacca aacaaaaaat aagtggttat aatgaatcgt taataagcaa
     1321 aattcattat aaccaaatta aagagggtta taatgaacga gaaaaatata aaacacagtc
     1381 aaaactttat tacttcaaaa cataatatag ataaaataat gacaaatata agattaaatg
     1441 aacatgataa tatctttgaa atcggctcag gaaaaggcca ttttaccctt gaattagtac
     1501 agaggtgtaa tttcgtaact gccattgaaa tagaccataa attatgcaaa actacagaaa
     1561 ataaacttgt tgatcacgat aatttccaag ttttaaacaa ggatatattg cagtttaaat
     1621 ttcctaaaaa ccaatcctat aaaatatttg gtaatatacc ttataacata agtacagata
     1681 taatacgcaa aattgttttt gatagtatag ctgatgagat ttatttaatc gtggaatacg
     1741 ggtttgctaa aagattatta aatacaaaac gctcattggc attactttta atggcagaag
     1801 ttgatatttc tatattaagt atggttccaa gagaatattt tcatcctaaa cctaaagtga
     1861 atagctcact tatcagatta aatagaaaaa aatcaagaat atcacacaaa gataaacaga
     1921 agtataatta tttcgttatg aaatgggtta acaaagaata caagaaaata tttacaaaaa
     1981 atcaatttaa caattcctta aaacatgcag gaattgacga tttaaacaat attagctttg
     2041 aacaattctt atctcttttc aatagctata aattatttaa taagtaagtt aagggatgca
     2101 taaactgcat cccttaactt gtttttcgtg tacctatttt ttgtgaatcg attatgtctt
     2161 ttgcgcattc gcttcttttc tatataaata tgagcgaagc gaataagcgt cggaaaagca
     2221 gcaaaaagtt tcctttttgc tgttggagca tgggggttca gggggtgcag tatctgacgt
     2281 caatgccgag cgaaagcgag ccgaagggta gcatttacgt tagataaccc cctgatatgc
     2341 tccgacgctt tatatagaaa agaagattca actaggtaaa atcttaatat aggttgagat
     2401 gataaggttt ataaggaatt tgtttgttct aatttttcac tcattttgtt ctaatttctt
     2461 ttaacaaatg ttcttttttt ttt
//
LOCUS       DAMJZE010000411         1448 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,14.7142552 isolate JH390
            SAMN32366204-rid16589573.guided.336, whole genome shotgun sequence.
ACCESSION   DAMJZE010000411 DAMJZE010000000
VERSION     DAMJZE010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366204
            Sequence Read Archive: SRR22862918
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560774.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560774.1
            Genome Coverage       :: 249x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:45:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,998
            CDSs (total)                      :: 3,912
            Genes (coding)                    :: 3,863
            CDSs (with protein)               :: 3,863
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 22 of 49
            Pseudo Genes (internal stop)      :: 19 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366204-rid16589573.guided.336"
                     /strain="L,14.7142552"
                     /isolate="JH390"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2013"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC541_003998"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC541_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2841354.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          615 a          115 c          237 g          481 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgtttt
//
